Welcome to yet another year of operation of the Microscopy Listserver. It was another productive year for all of you, during 2008, the listserver delivered 2238 messages (} 230 Gb of Email) to ~ 3000 subscribers around the world, with only minimal hassels (that I know about).
The complete Microscopy Listserver Archives for 2008-1993 are on-line at http://www.microscopy.com. Those of you that use the archive facility will notice I have decided to no longer produce monthly snap shots of the posting for the search engines. Instead you will only be able to search a full year at a time. I no longer believe these smaller monthly snapshots are important with the ubiquitous access to high speed network connections.
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Thu Jan 1 08:37:35 2009 6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n01EbYjw021867 6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2009 08:37:34 -0600 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240800c582825929fb-at-[206.69.208.22]} 6, 11 -- Date: Thu, 1 Jan 2009 08:37:32 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 6, 11 -- Subject: Administrivia: Listserver Archives for 2008 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
One stain is tannic acid/ferric chloride, it's a good alternative to osmium. I've usually used this for membranes, but it does stain things like cytoskeletal proteins and ribosomes pretty well too - it probably binds to certain charged surfaces or groups, like most fixatives.
You can do this a number of ways, the two commonest ones are to either fix first in aldehyde (glutaraldehyde or formaldehyde) in appropriate buffer, rinse well, then stain in 1% tannic acid, rinse well, then stain in 1% ferric chloride. The tissue will go black, just as if it has been osmicated. Then continue as usual - dehydrate, embed.
The alternative is to add the tannic acid in with the aldehyde fixative, which is the way I've usually used it for plant tissues.
The ferric chloride is just made up in distilled water, and you can vary the concentration of either TA or FeCl3, and vary the time, etc.
I imagine you are fixing animal/human tissue (I work on plants only), so just go with whatever is the standard TEM protocol and add the TA/FeCl3 as above. It used to be the case that for reliable results you had to use Mallinkrodt tannic acid, but the manufacturing process used by other companies may be better now.
There are a couple of older references to this which I can dig out - the paper we cite them in is pre-web.....
cheers, from 2009 already downunder... Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 1/01/09 7:46 AM, "twigg-at-estd.nrl.navy.mil" {twigg-at-estd.nrl.navy.mil} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both twigg-at-estd.nrl.navy.mil as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: twigg-at-estd.nrl.navy.mil } Name: Mark Twigg } } Organization: Naval Research Laboratory } } Title-Subject: [Filtered] Stains for proteins } } Question: I have a new project which may entail staining proteins so } that there is more contrast for TEM imaging. One collaborator } recommended osmium tetroxide, but I read on the web that it is rather } poisonous and requires special handling. I have not done this sort } of biological TEM before and I would appreciate any tips or } references. } } Thanks, } } Mark } } Login Host: 132.250.134.121 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Wed Dec 31 14:38:46 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mBVKcjqv024150 } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 31 Dec 2008 14:38:45 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c58188c4a985-at-[206.69.208.22]} } 8, 11 -- Date: Wed, 31 Dec 2008 14:38:45 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Stains for proteins } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
Developing a new class is always difficult but more so when you are still in the process of setting up the lab. Regarding your digital question, we are a major research facility that still uses primarily film. Our users come from all areas of the university and have a very wide range of sample types requiring an equally wide range of preparation techniques and original magnifications. I have two equivalent TEMs with one having a 4k digital camera. The camera is rarely used. Why?
For a number of reasons. One is that TEM is a very poor sampling technique. We look at extremely small samples and then extrapolate to the whole. A digital camera, especially a high-resolution bottom mounted one, captures a very small area of the total screen. This makes sampling worse. Yes you can reduce primary magnification to capture a larger area but then the camera is doing the magnification rather than the TEM lenses. This is fine for very low magnification but is not as desirable for higher mag. You can take more digital images but this is time consuming and may not be as informative as a single larger area.
Also, especially when dealing with samples that have a very wide grayscale range, such as negatively stained samples where you often have intense dark areas along with quite light ones, it is often much more difficult to prevent oversaturation with a digital camera due to the enhanced contrast. It often takes longer to get a good digital image as you try to find correct camera settings than to take a negative. You have a hardcopy of your data with a negative that then allows you to scan the negative at whatever resolution value is desirable to get the enlargement you need without being limited to the pixel size of the initial digital image.
Yes there are advantaged to going totally digital. Not having to deal with expense of film and the time for the developing (~20min for 30-40negatives plus some wash time) is preferable to some. You can check focus on the screen and toss any image that is not just right. You need to be able to accurately focus when using film to minimize loss (but I consider this a part of learning to be a good microscopist). You need less beam exposure so this can help with fragile samples. It is easier to work with low contrast samples due to increased camera-provided contrast. I am sure there are many other advantages as well, providing you can afford the initial significant expense of purchasing the camera in the first place.
Our compromise is to teach students to recognize focus and learn to stigmate at high magnification on the screen. Once they "get" this, they can then focus and stigmate whenever and where ever needed with minimal time and effort and get a very high percentage of usable negatives. Time on the scope is normally less than when taking digital images so actual cost (scope time + negatives) is about equal. Students then scan their negatives at resolution desired and have their digital images. We haven't printed at all in about 4 years and only rarely for about 4 years previously to that. Consumer scanners with transmitted light (under $700) do fine for this purpose as do inkjet printers although students prefer to look at images on screen and rarely print them.
There is certainly a place for digital cameras, especially when you are taking low mag images and need to get results in a hurry such as in a diagnostic situation. It is very helpful for the occasional user who has problems focusing well and can be quite adequate/desirable for other needs, such as electron diffraction, as you can get a good dynamic range. Just do not feel that this is a must purchase for an undergraduate course when the $$ can perhaps better go to other preparation equipment or supplies.
Regarding use of nitrogen...I admit that I am old school. Lots of samples are very stable under the beam and contamination is not a hugh problem with modern pumping systems...especially at low magnifications where reductions in resolution due to contamination are not easily visible. But as soon as we say that, someone will come along with a sample that is not stable and you end up with a lot of contamination released into the column and adverse effects as a result. Part of keeping a common facility up and running is to train all users to do things in the same methodical manner. This greatly reduces user error. I would rather err on the side of caution and require everyone to use nitrogen than hope that students will recognize when it is desirable. THAT WON"T HAPPEN!
My recommendation is to get two 25L dewars so that one can be off getting filled while the other is available as needed. You will probably only have to fill once every few weeks or so and hopefully there is a larger tank some where on campus to make this possible.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
On 12/31/08 7:32 PM, "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } It's been a few years since I've been on the list server - doing those } recommended postdocs to "broaden my horizons" beyond microscopy (I'm wondering } if I should have followed that advice!). } } I'm gearing up to teach an EM class for biologists to undergrads. The class } hasn't been taught at my university in eons, so I'm basically starting from } scratch with a great set of brand new JEOL scopes and a lab full of virgin } equipment. It would be a dream come true if not for the fact that I've got to } learn how to use all of this brand new equipment in short order! } } I'm sure that I'll be in touch throughout the upcoming semester, but for now, } I have a few questions on which I'd like your learned advice: } } 1. Does anyone have experience with a JEOL JEM1011 TEM? Thoughts on it? Do } you have an abbreviated user's protocol that you would be willing to share? No } one here has ever used this brand new (though 5 year old scope), and, though } JEOL will graciously come and do a training session with me, I'd love any } input you may have. } } 2. Going digital. We are looking at getting a digital image capture system. } I'm old enough to have been trained in the darkroom long ago. Should I teach } these students darkroom technique or just assume that they're in the digital } age and go with either digital capture or digital image manipulation (scanning } in EM negatives and printing)? Vote and let me know. And, if you've got a } camera system, scanner or printer that you would recommend, that would be } great. } } 3. Lastly, and this may show the old workhorse microscopes I was weaned on - } LN2. I've been advised to just forget about using LN2 with this TEM for } biological applications. Really? Granted, I'll talk to the JEOL rep about } this, but if you've got thoughts on it, please share. I hear that getting LN2 } into our lab may be a problem, but my gut tells me that I should make ripples } with this one. } } 4. Okay, one more, but it's an easy one. Does anyone have a recommendation for } a quick and easy animal tissue to use for teaching? I'm a die hard plant } person (perfusion - ahh!), but I think that not having experience handling and } looking at animal tissue has been a handicap for me. I don't want that for my } students. Can I just go pick up some chicken livers or something and not have } to find something to sacrifice? } } That's it for now. I know that there will be more. } } Many thanks, } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } k.lennon-at-frostburg.edu } } } } } ==============================Original Headers============================== } 13, 20 -- From kamlennon-at-yahoo.com Wed Dec 31 18:30:31 2008 } 13, 20 -- Received: from web84007.mail.mud.yahoo.com } (web84007.mail.mud.yahoo.com [68.142.206.177]) } 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } n010UVQW011058 } 13, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 31 Dec 2008 18:30:31 -0600 } 13, 20 -- Received: (qmail 23609 invoked by uid 60001); 1 Jan 2009 00:30:30 } -0000 } 13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 13, 20 -- s=s1024; d=yahoo.com; } 13, 20 -- } h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Con } tent-Type:Message-ID; } 13, 20 -- } b=CqDVTUJAqH9OmwKIT05TcFhilN8W5b4TH4VVVw3XGiIuPorbXVxCisHm0it7iPSLaS9sjsY/Lhme } JNa3JpJo/lOiIKMqBLserDqmQ1+3yFel3/IfzViKtTyyx4AUo6roq5dZ4iDp7nUrjnWr7aoXqkXozP } M4YrBTri8ScUff9eU=; } 13, 20 -- X-YMail-OSG: } KuV9JQgVM1kqFtyeJQ5xhd81MO9MDtVbiXwLAuz7_j.epu5Np3DdPvW8kKX1n_x9ZA-- } 13, 20 -- Received: from [96.239.149.81] by web84007.mail.mud.yahoo.com via } HTTP; Wed, 31 Dec 2008 16:30:30 PST } 13, 20 -- X-Mailer: YahooMailWebService/0.7.247.3 } 13, 20 -- Date: Wed, 31 Dec 2008 16:30:30 -0800 (PST) } 13, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } 13, 20 -- Reply-To: kamlennon-at-yahoo.com } 13, 20 -- Subject: Developing EM class for undergrads + going digital advice? } 13, 20 -- To: Microscopy-at-Microscopy.com } 13, 20 -- MIME-Version: 1.0 } 13, 20 -- Content-Type: text/plain; charset=us-ascii } 13, 20 -- Message-ID: {295847.22686.qm-at-web84007.mail.mud.yahoo.com} } ==============================End of - Headers==============================
==============================Original Headers============================== 18, 32 -- From dsherman-at-purdue.edu Thu Jan 1 10:23:12 2009 18, 32 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n01GNBma021014 18, 32 -- for {microscopy-at-microscopy.com} ; Thu, 1 Jan 2009 10:23:11 -0600 18, 32 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 18, 32 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n01GNAEQ002896; 18, 32 -- Thu, 1 Jan 2009 11:23:10 -0500 18, 32 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 18, 32 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n01GNASW018749; 18, 32 -- Thu, 1 Jan 2009 11:23:10 -0500 18, 32 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 18, 32 -- Thu, 1 Jan 2009 11:23:09 -0500 18, 32 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 18, 32 -- Thu, 1 Jan 2009 16:22:35 +0000 18, 32 -- User-Agent: Microsoft-Entourage/12.15.0.081119 18, 32 -- Date: Thu, 01 Jan 2009 11:22:34 -0500 18, 32 -- Subject: Re: [Microscopy] Developing EM class for undergrads + going digital 18, 32 -- advice? 18, 32 -- From: Debby Sherman {dsherman-at-purdue.edu} 18, 32 -- To: {kamlennon-at-yahoo.com} , "message to: MSA list" {microscopy-at-microscopy.com} 18, 32 -- Message-ID: {C58257FA.2535C%dsherman-at-purdue.edu} 18, 32 -- Thread-Topic: [Microscopy] Developing EM class for undergrads + going digital 18, 32 -- advice? 18, 32 -- Thread-Index: AclsLSb5DjLGwKQiCUGlT29ZAf6ywQ== 18, 32 -- In-Reply-To: {200901010032.n010WP7K014214-at-ns.microscopy.com} 18, 32 -- Mime-version: 1.0 18, 32 -- Content-type: text/plain; 18, 32 -- charset="US-ASCII" 18, 32 -- Content-transfer-encoding: 7bit 18, 32 -- X-OriginalArrivalTime: 01 Jan 2009 16:23:09.0837 (UTC) FILETIME=[3C560BD0:01C96C2D] 18, 32 -- X-PMX-Version: 5.4.0.320885 18, 32 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
I'm sure the government was regulations and requirements which make using osmium tetroxide difficult, but like most chemical, osmium tetroxide can be safely used. At rubber company in Akron we used a water dilution of osmium tetroxide both as vapor phase to stain thin sections of unsaturated rubber and added it to latex rubber has a hardener. We worked in a chemical hood, wore thin nitrile rubber gloves used normal chemical practices and never experienced a problem.
The vials crystal osmium were scored, broken and dropped into distilled water (we used a taped wrapped bottle to protect the solution from light) and small amounts were removed from the bottle with clean pasture pipets. The used solutions were decanted into a open wide mouth bottle stored in the back of the hood. The water evaporated, the osmium reacted with dust and organic material in the air and once a year we properly discarded the sludge. We also placed the used TEM grids, used vials and latex solutions in that bottle.
If you get the results you want from other stains that's great. But don't let scary internet precautions and many of the warnings we read persuade you from using chemicals.
Too often, people with limited chemical experience and education make difficult rules not to protect you, but to protect the organization from imagined legal complications. These people are not charged with solving the problems your are responsible for and have limited if any sympathy if you are unable to achieve these goals due to their restrictions.
twigg-at-estd.nrl.na vy.mil To 12/31/2008 03:48 frank_karl-at-lincolnelectric.com PM cc
Subject Please respond to [Microscopy] viaWWW: Stains for twigg-at-estd.nrl.na proteins vy.mil
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] Stains for proteins
Question: I have a new project which may entail staining proteins so that there is more contrast for TEM imaging. One collaborator recommended osmium tetroxide, but I read on the web that it is rather poisonous and requires special handling. I have not done this sort of biological TEM before and I would appreciate any tips or references.
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Wed Dec 31 14:38:46 2008 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBVKcjqv024150 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 31 Dec 2008 14:38:45 -0600 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p06240800c58188c4a985-at-[206.69.208.22]} 8, 11 -- Date: Wed, 31 Dec 2008 14:38:45 -0600 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: Stains for proteins 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 27, 22 -- From frank_karl-at-lincolnelectric.com Fri Jan 2 06:12:27 2009 27, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 27, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02CCRJv027990 27, 22 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2009 06:12:27 -0600 27, 22 -- In-Reply-To: {200812312048.mBVKmOhZ004457-at-ns.microscopy.com} 27, 22 -- Subject: Re: [Microscopy] viaWWW: Stains for proteins 27, 22 -- To: twigg-at-estd.nrl.navy.mil, Microscopy-at-microscopy.com 27, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 27, 22 -- Message-ID: {OF639C253E.DA709F59-ON85257532.0040B9C6-85257532.00430824-at-lincolnelectric.com} 27, 22 -- Date: Fri, 2 Jan 2009 07:12:12 -0500 27, 22 -- From: Frank_Karl-at-lincolnelectric.com 27, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 27, 22 -- 07, 2008) at 01/02/2009 07:12:13 AM, 27, 22 -- CD-MIME complete at 01/02/2009 07:12:13 AM, 27, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 27, 22 -- 07, 2008) at 01/02/2009 07:12:13 AM, 27, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 27, 22 -- 07, 2008) at 01/02/2009 07:12:13 AM, 27, 22 -- Serialize complete at 01/02/2009 07:12:13 AM 27, 22 -- MIME-Version: 1.0 27, 22 -- Content-Type: text/plain; 27, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
2. Going digital. We are looking at getting a digital image capture system. I'm old enough to have been trained in the darkroom long ago. Should I teach these students darkroom technique or just assume that they're in the digital age and go with either digital capture or digital image manipulation (scanning in EM negatives and printing)? Vote and let me know. And, if you've got a camera system, scanner or printer that you would recommend, that would be great.
Hi Kristen, I've used glass plates, cut film and digital imaging. I love the darkroom work as well as the incredible images one gets from printing negatives with the right "hardness" of paper, developer time and chemistry. With true dedication you could take 20 images with the TEM, drop the vacuum, remove the cassette, replace the cassette with a pre-pumped one (to lower the water content and make your TEM pump down faster). If the negative chemistry was at the proper temperature and not used up, you could develop, dry the negative, then calibrate the enlarger, make sure the paper chemistry was hot and not used up, determine the correct exposure, (it always seemed to vary from negative to negative), print, develop, then repeat with dodging or burning the image to bring out the details you need, dry the paper, (used RC paper so you don't need a print dryer) and in eight hours you could have two or three exceptional copies of each of the 20 negatives. (OH! I forgot make sure you record and identify the negatives and store them so in twenty years someone will have several 100 pounds of polyester film to dispose of.)
Or you could focus the microscope, move a lever or two, capture the image, print it on a quality printer, decide if you want the image, correct the exposure and composition and take another images. Yes, they are not as nice as "photographs". But with the right paper, and a quality camera (that means lots of bucks, pounds or yen) you can get an images 99% as good and all in less then 3 minutes.
Digital, I dislike it, but it is the way to go........,
Stay safe........ Frank Karl....... microscopist and former darkroom junkie
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==============================Original Headers============================== 8, 23 -- From frank_karl-at-lincolnelectric.com Fri Jan 2 06:37:14 2009 8, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02CbCiG015828 8, 23 -- for {microscopy-at-microscopy.com} ; Fri, 2 Jan 2009 06:37:13 -0600 8, 23 -- In-Reply-To: {200901010037.n010ba2u023805-at-ns.microscopy.com} 8, 23 -- Subject: Re: [Microscopy] Developing EM class for undergrads + going digital advice? 8, 23 -- Sensitivity: 8, 23 -- To: kamlennon-at-yahoo.com, Microscopy-at-microscopy.com 8, 23 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 8, 23 -- Message-ID: {OF1BAFA938.E4D83FCB-ON85257532.00432A06-85257532.004548CB-at-lincolnelectric.com} 8, 23 -- Date: Fri, 2 Jan 2009 07:36:48 -0500 8, 23 -- From: Frank_Karl-at-lincolnelectric.com 8, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 23 -- 07, 2008) at 01/02/2009 07:36:49 AM, 8, 23 -- CD-MIME complete at 01/02/2009 07:36:49 AM, 8, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 23 -- 07, 2008) at 01/02/2009 07:36:49 AM, 8, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 23 -- 07, 2008) at 01/02/2009 07:36:49 AM, 8, 23 -- Serialize complete at 01/02/2009 07:36:49 AM 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="US-ASCII" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Rod-at-RJAndA.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Rod-at-RJAndA.com Name: Rod Johnson
Organization: Rod Johnson & Associates, Inc.
Title-Subject: [Filtered] JEOL top reference holders
Question: Happy New Year!
We purchased a used JEOL 840 this year. We are looking for 1 1/4 inch top reference holders. If you have holders you are no longer using we would be pleased to pay for them and their shipping.
Here is the January 2009 Microscopy Today table of contents. We will close the subscription list for this issue on Wednesday, January 7, 2009. Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Technical Editor ===================== Tiny Bubbles Stephen W. Carmichael, Mayo Clinic
New Large Area Silicon Drift Detectors - Fast Analysis without Compromise Clair Collins, Neil Rowlands, Peter Statham, and James Holland, Oxford Instruments, High Wycombe, Bucks, England
Microscopy Today New Publication Directions Ron Anderson and Charles Lyman,*Microscopy Today, Largo, FL and *Lehigh University Bethlehem, PA
Manufacturer Training of Electron Microscopy and Analysis Techniques Neil Rowlands, Oxford Instruments, Concord, MA
Remote Microscopy for Education and Outreach S. Seraphin, S. Hernandez, G. Chandler, D. Bentley*, K. Dorame, M. Sellers,** Univ. of Arizona, Tucson, AZ, * ** N. Arizona Univ., Flagstaff, AZ
The Electron Microscopy Database: an Online Resource for Teaching and Learning Quantitative Transmission Electron Microscopy Paul M. Voyles, Department of Materials Science and Engineering, University of Wisconsin, Madison, Madison, WI
SEM Short Courses for Industry: the Lehigh Microscopy School as an example Charles E. Lyman, Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA
Direct Visualisation, Sizing and Counting of Virus and Phage Particles in Liquids Bob Carr, and Duncan Griffiths,* NanoSight Ltd., Salisbury, UK, *NanoSight USA, Costa Mesa, CA
Pioneers in Optics: Ernst Abbe (1840-1905) Michael W. Davidson, The Florida State Univ., Tallahassee, FL
Single-Molecule DNA Stretching Using Optical Tweezers Joost van Mameren, Anna Wozniak, and Sid Ragona,* JPK Instruments, Berlin, Germany, *Ragona Scientific, Pittsford, NY
Event Streamed Spectrum Imaging using Programmed Beam Acquisition in Biological Microprobe Analysis P. Ingram,* S. D. Davilla,** & A. LeFurgey*, *Duke Univ. and Veterans Affairs Med. Ctr, Durham, NC, **4pi Analysis Inc., Durham, NC
RGB-Splitting and Multi-Shot Techniques in Digital Photomicrography–Utilization of Astronomic RGB-Filters in True Color Imaging Jörg Piper, Clinic “Meduna,” Bad Bertrich, Germany
Preventing the Sale of Fraudulent Gemstones using Non-Destructive X-Ray Fluoresence Spectroscopy Mary S. Goldman, Dan L. Davis, Robert H. Clifford, Shimadzu Scientific Instruments Inc., Columbia, MD
Industry News
NetNotes SPECIMEN PREPARATION - glutaraldehyde shelf life SPECIMEN PREPARATION – Spurr’s resin SPECIMEN PREPARATION – processing paraffin specimens for TEM MICROTOMY – flattening sections MICROTOMY – wetting the knife MICROTOMY - coated grids IMMUNOCYTOCHEMISTRY - fluorescence quenching IMAGE PROCESSING - reference image subtraction TEM – image distortion TEM – comparison with STEM SEM – backscattering detector image formation SEM – backscatter detector SEM - Coating for focused ion beam (FIB) SEM SEM – active and passive acquisition EM - SF6 detector EM - CTF function EM – pump speed vs. ultimate pressure EM - plasma cleaner SEM – oil shale rock sample preparation SEM - of paper
Dear Abbe
Advertiser's Index
==============================Original Headers============================== 20, 17 -- From randerson20-at-tampabay.rr.com Fri Jan 2 16:20:44 2009 20, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 20, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n02MKiqJ029455 20, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 2 Jan 2009 16:20:44 -0600 20, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 20, 17 -- by hrndva-omta01.mail.rr.com with ESMTP 20, 17 -- id {20090102222042.RAZV6232.hrndva-omta01.mail.rr.com-at-[127.0.0.1]} 20, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 2 Jan 2009 22:20:42 +0000 20, 17 -- Message-ID: {495E9335.8050806-at-tampabay.rr.com} 20, 17 -- Date: Fri, 02 Jan 2009 17:20:37 -0500 20, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 20, 17 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 20, 17 -- MIME-Version: 1.0 20, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 20, 17 -- Subject: January 2009 Microscopy Today Table of Contents 20, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We have the following equipment that we would like to find new homes for: Lynx EL Tissue Processor and supplies Digital Dryer (brand new, table top) Arkay CD 20 dryer 2-Codonics NP 1600 printers (brand new with supplies)
Photos are available upon request.
Priced as a donation to Valley Catholic High School EM Lab and shipping costs, take one or all!
Thank you,
Hobie
Hobie Richards Partner, and COO Technical Sales Solutions, LLC Portland, OR USA www.TechnicalSalesSolutions.com 503 781 0428
Skype Hobie-TSS
==============================Original Headers============================== 11, 21 -- From Hobie-at-technicalsalessolutions.com Sat Jan 3 13:21:10 2009 11, 21 -- Received: from host203.com (host203.com [203.194.159.243]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n03JL8xC008261 11, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 13:21:09 -0600 11, 21 -- Received: (qmail 8983 invoked by uid 503); 3 Jan 2009 19:21:05 -0000 11, 21 -- Received: from unknown (HELO ?10.0.1.195?) (Hobie-at-76.115.10.232) 11, 21 -- by host203.com with ESMTPA; 3 Jan 2009 19:21:05 -0000 11, 21 -- User-Agent: Microsoft-Entourage/12.0.0.071130 11, 21 -- Date: Sat, 03 Jan 2009 11:20:58 -0800 11, 21 -- Subject: Extra Lab Items 11, 21 -- From: Hobie Richards {Hobie-at-technicalsalessolutions.com} 11, 21 -- To: {Microscopy-at-microscopy.com} 11, 21 -- Message-ID: {C584FA9A.16024%Hobie-at-technicalsalessolutions.com} 11, 21 -- Thread-Topic: Extra Lab Items 11, 21 -- Thread-Index: AcltJOhHkhtkCvXFM0ae5WxF7iwr5wAAVa5wACyKOCs= 11, 21 -- In-Reply-To: {C583CFB3.15FF3%Hobie-at-technicalsalessolutions.com} 11, 21 -- Mime-version: 1.0 11, 21 -- Content-type: text/plain; 11, 21 -- charset="ISO-8859-1" 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n03JL8xC008261 ==============================End of - Headers==============================
Tracor Northern: PAC Control Console Joystick/Keypad MicroScan Trackball/Slide Controller 2) Programmable Auto Controllers (steel boxes with boards) Connector Box
We came across the above surplus items we acquired and never used. Not wanting to throw these out if anyone can use them, we will happily box them up for you. They will be yours for the cost of shipping.
Alan Stone ASTON Metallurgical Services Co., Inc. Wheeling, IL
==============================Original Headers============================== 6, 19 -- From as-at-astonmet.com Sat Jan 3 14:52:49 2009 6, 19 -- Received: from outbound1.mail.tds.net (outbound1.mail.tds.net [216.170.230.91]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n03Kqm16023434 6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 14:52:49 -0600 6, 19 -- Received: from outaamta01.mail.tds.net (outaamta01.mail.tds.net [216.170.230.31]) 6, 19 -- by outbound1.mail.tds.net (8.13.6/8.13.4) with ESMTP id n03KqlMP023000 6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 14:52:47 -0600 6, 19 -- Received: from OFFICE.astonmet.com ([69.128.246.226]) 6, 19 -- by outaamta01.mail.tds.net with ESMTP 6, 19 -- id {20090103205247.VXVJ26415.outaamta01.mail.tds.net-at-OFFICE.astonmet.com} 6, 19 -- for {microscopy-at-microscopy.com} ; Sat, 3 Jan 2009 14:52:47 -0600 6, 19 -- Message-Id: {6.2.0.14.2.20090103144053.02423dd0-at-pop.tds.net} 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 6, 19 -- Date: Sat, 03 Jan 2009 14:51:33 -0600 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- From: Alan Stone {as-at-astonmet.com} 6, 19 -- Subject: Cleaning House......Tracor Northern Parts 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Technological University
Title-Subject: [Filtered] wanted - 4pi SE II electronics
Question: We'd like to buy (a donation would not hurt my feelings :} ) a 4pi SE II x-ray system. I really only need the control box, PCI slot card and cables since we have a working detector and HV power supply. Please contact me off-line at opmills-at-mtu.edu.
International Conference on PROCESSING & MANUFACTURING OF ADVANCED MATERIALS Processing, Fabrication, Properties, Applications August 25-29, 2009, Technical University-Berlin, Germany www.thermec.uow.edu.au
best regards KJ Hübner
____________ Virus checked by G DATA AntiVirus Version: AVF 19.204 from 29.12.2008 Virus news: www.gdata.pl
==============================Original Headers============================== 5, 26 -- From hubner-at-iod.krakow.pl Mon Jan 5 05:48:55 2009 5, 26 -- Received: from sowa.iod.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n05BmsD2031012 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 05:48:55 -0600 5, 26 -- Received: from SERWER_SQL (serwer_sql [149.156.29.202]) 5, 26 -- by sowa.iod.krakow.pl (8.13.6+Sun/8.13.6) with SMTP id n05BRhvs015278 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 12:27:43 +0100 (CET) 5, 26 -- Received: from [149.156.29.4] (helo=iodkh007) 5, 26 -- by SERWER_SQL with AVK MailGateway; 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 05 Jan 2009 12:50:21 +0100 5, 26 -- Date: Mon, 5 Jan 2009 12:48:53 +0100 5, 26 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-iod.krakow.pl} 5, 26 -- To: "Microscopy list" {microscopy-at-microscopy.com} 5, 26 -- MIME-Version: 1.0 5, 26 -- Message-ID: {8378428096D94ACBA990809C5149F148-at-iodkh007} 5, 26 -- Subject: conference Thermec 2009 Berlin 5, 26 -- Content-Type: text/plain; 5, 26 -- charset="iso-8859-2"; 5, 26 -- format="flowed"; 5, 26 -- reply-type="original" 5, 26 -- Content-Transfer-Encoding: 8bit 5, 26 -- X-Priority: 3 5, 26 -- X-MSMail-Priority: Normal 5, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 5, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 5, 26 -- X-AVK-Virus-Check: AVF 19.204;29.12.2008 ==============================End of - Headers==============================
Hope this isn't too late - CMU shuts down over Christmas.
Good luck with the new class. Undergrads can be much better at things like EM than people give them credit for (from experience with our EM classes). I can't help you with the JEOL, we have a Philips, but more generally:
2. Do both. Shoot film negatives and digital. Debby Sherman has already provided excellent reasons for keeping the film negatives, so I won't repeat those. I think it's worthwhile doing digital imaging in addition because first, many labs are going that way, and you have an opportunity to train students in the proper use of a digital system, second, as Debby mentioned, it's cheaper to operate (no chemicals, etc.) and so a useful system for teaching focus and stigmation, and for quick checks of sections. If this is an either-or decision, keep the film. The digital system can be learned whereever the students go.
4. Crickets. We use cricket tissues in our TEM class. The femur provides plenty of muscle, which has lots of interesting and easily identifiable structures. Plus, it allows some basic study of structure/ function, something that is missing from many microscopy classes and shouldn't be. Fix the tissue in contracted and stretched conditions, for example. Further, there are lots of other interesting tissues: midgut, ventral nerve cord, brain, Malphigian tubules, and so on. Also, since many sections will contain tracheoles, there is another chance to discuss physiology and microanatomy.
Phil
} Hi All, } } It's been a few years since I've been on the list server - doing } those recommended postdocs to "broaden my horizons" beyond } microscopy (I'm wondering if I should have followed that advice!). } } I'm gearing up to teach an EM class for biologists to undergrads. } The class hasn't been taught at my university in eons, so I'm } basically starting from scratch with a great set of brand new JEOL } scopes and a lab full of virgin equipment. It would be a dream come } true if not for the fact that I've got to learn how to use all of } this brand new equipment in short order! } } I'm sure that I'll be in touch throughout the upcoming semester, but } for now, I have a few questions on which I'd like your learned } advice: } } 1. Does anyone have experience with a JEOL JEM1011 TEM? Thoughts on } it? Do you have an abbreviated user's protocol that you would be } willing to share? No one here has ever used this brand new (though 5 } year old scope), and, though JEOL will graciously come and do a } training session with me, I'd love any input you may have. } } 2. Going digital. We are looking at getting a digital image capture } system. I'm old enough to have been trained in the darkroom long } ago. Should I teach these students darkroom technique or just assume } that they're in the digital age and go with either digital capture } or digital image manipulation (scanning in EM negatives and } printing)? Vote and let me know. And, if you've got a camera system, } scanner or printer that you would recommend, that would be great. } } 3. Lastly, and this may show the old workhorse microscopes I was } weaned on - LN2. I've been advised to just forget about using LN2 } with this TEM for biological applications. Really? Granted, I'll } talk to the JEOL rep about this, but if you've got thoughts on it, } please share. I hear that getting LN2 into our lab may be a problem, } but my gut tells me that I should make ripples with this one. } } 4. Okay, one more, but it's an easy one. Does anyone have a } recommendation for a quick and easy animal tissue to use for } teaching? I'm a die hard plant person (perfusion - ahh!), but I } think that not having experience handling and looking at animal } tissue has been a handicap for me. I don't want that for my } students. Can I just go pick up some chicken livers or something and } not have to find something to sacrifice? } } That's it for now. I know that there will be more. } } Many thanks, } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } k.lennon-at-frostburg.edu
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 8, 26 -- From oshel1pe-at-cmich.edu Mon Jan 5 10:12:43 2009 8, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n05GCh4d019121 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 10:12:43 -0600 8, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 8, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n05GCa96007385 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jan 2009 11:12:42 -0500 8, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Mon, 5 Jan 2009 11:12:19 -0500 8, 26 -- Mime-Version: 1.0 8, 26 -- Message-Id: {f06240808c587debf7f93-at-[141.209.160.249]} 8, 26 -- In-Reply-To: {200901010034.n010Y3AT016917-at-ns.microscopy.com} 8, 26 -- References: {200901010034.n010Y3AT016917-at-ns.microscopy.com} 8, 26 -- Date: Mon, 5 Jan 2009 11:12:17 -0500 8, 26 -- To: Microscopy-at-microscopy.com 8, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 8, 26 -- Subject: Re: [Microscopy] Developing EM class for undergrads + going 8, 26 -- digital advice? 8, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 26 -- X-OriginalArrivalTime: 05 Jan 2009 16:12:19.0226 (UTC) FILETIME=[6231AFA0:01C96F50] 8, 26 -- X-Canit-CHI2: 0.00 8, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 8, 26 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 8, 26 -- X-CanItPRO-Stream: default 8, 26 -- X-Canit-Stats-ID: 7064876 - 02ca6d00f197 8, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
We are doing BiFC to study interaction of two nucleoporins in vivo first and then at the em level using immunogold labeling. Does anyone know, if there is a GFP antibody available that would only recognize the two halves when assembled? Thanks for your help.
Tea -- *************************************** Tea Meulia, PhD Research Scientists and Director Molecular and Cellular Imaging Center Ohio State University/OARDC 1680 Madison Ave. Wooster OH 44691
tel.: 330-263-3836 or -3828 fax: 330-202-3563
http://www.oardc.ohio-state.edu/mcic
*****************************************
==============================Original Headers============================== 5, 21 -- From meulia.1-at-osu.edu Tue Jan 6 09:54:23 2009 5, 21 -- Received: from defang19.it.ohio-state.edu (defang19.it.ohio-state.edu [128.146.216.133]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n06FsNR8008224 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Jan 2009 09:54:23 -0600 5, 21 -- Received: from defang9.it.ohio-state.edu (defang9.it.ohio-state.edu [128.146.216.78]) 5, 21 -- by defang19.it.ohio-state.edu (8.13.7/8.13.1) with ESMTP id n06FsMaT007775 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Jan 2009 10:54:22 -0500 5, 21 -- Received: from [140.254.186.120] (dhcp-140-254-186-120.osuwireless.ohio-state.edu [140.254.186.120]) 5, 21 -- by defang9.it.ohio-state.edu (8.13.7/8.13.1) with ESMTP id n06FsM4c027286 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 6 Jan 2009 10:54:22 -0500 5, 21 -- Mime-Version: 1.0 5, 21 -- Message-Id: {p06240807c5892d4e19f3-at-[140.254.186.120]} 5, 21 -- Date: Tue, 6 Jan 2009 10:57:16 -0500 5, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 5, 21 -- From: Tea Meulia {meulia.1-at-osu.edu} 5, 21 -- Subject: antibodies for BiFC 5, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 21 -- X-Spam-Score: 0.00 () [Tag at 4.50] 5, 21 -- X-CanItPRO-Stream: outbound 5, 21 -- X-Canit-Stats-ID: Bayes signature not available 5, 21 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.146.216.133 ==============================End of - Headers==============================
We have just received this message from Ash Prabala, President and CEO of DVC company:
"It is with profound regret that I inform you that Doug Benson, a dear friend and colleague, passed away early this morning at the age of 56. It is very difficult to find a silver lining in this news, but given the tough odds that he faced, at least he was spared a prolonged battle with cancer. Also, several generations of his family were close by and I know that he was greatly comforted by their proximity, and by their warmth and love.
Prior to his pivotal role as Chief Scientist and Director at DVC, Doug was the founder of Inovision Corporation - a highly regarded industry leader in the development of image analysis software. Before that, he was a National Cancer Institute Fellow at the Baylor College of Medicine in Houston, Texas. Douglas obtained a Ph.D. in Biochemistry from North Carolina State University.
I know that there are many in the Life Sciences, Microscopy & Imaging communities who knew Doug well, either personally or through his academic, research and/or business affiliations. Please keep Doug, his wife Louise, and their family members in your thoughts. I feel a deep sense of personal loss and sorrow, but will always remember Doug as a warm, generous, funny, brilliant, caring friend and colleague. I will miss his sense of humor and the twinkle in his eyes when he would recount a joke or a personal anecdote.
Rest In Peace, Doug. You will be missed by all those who had the privilege of knowing you."
We have just received this message from Ash Prabala, President and CEO of DVC company:
"It is with profound regret that I inform you that Doug Benson, a dear friend and colleague, passed away early this morning at the age of 56. It is very difficult to find a silver lining in this news, but given the tough odds that he faced, at least he was spared a prolonged battle with cancer. Also, several generations of his family were close by and I know that he was greatly comforted by their proximity, and by their warmth and love.
Prior to his pivotal role as Chief Scientist and Director at DVC, Doug was the founder of Inovision Corporation - a highly regarded industry leader in the development of image analysis software. Before that, he was a National Cancer Institute Fellow at the Baylor College of Medicine in Houston, Texas. Douglas obtained a Ph.D. in Biochemistry from North Carolina State University.
I know that there are many in the Life Sciences, Microscopy & Imaging communities who knew Doug well, either personally or through his academic, research and/or business affiliations. Please keep Doug, his wife Louise, and their family members in your thoughts. I feel a deep sense of personal loss and sorrow, but will always remember Doug as a warm, generous, funny, brilliant, caring friend and colleague. I will miss his sense of humor and the twinkle in his eyes when he would recount a joke or a personal anecdote.
Rest In Peace, Doug. You will be missed by all those who had the privilege of knowing you."
NOTE: DO NOT REPLY TO THIS EMAIL. SEE BELOW FOR APPLICATION INFORMATION.
Electron Microscopy Position Available Official Title: Molecular Technologist II, III, or IV--depending on qualifications and work experience
Location: Duke University Medical Center, Durham, NC
Requirements: Training and experience in running and maintaining electron microscopes, proficiency in cutting ultrathin sections and performing negative staining. Knowledge of scientific laboratory operation (making solutions, ordering, typing results, keeping records, etc.). Clinical laboratory and research experience are a plus.
Laboratory description: The work force consists of the director and 6 EM technologists who perform pathology (~500 samples/year), virology (~1000 samples/year), and research work; 3 TEMs; 1 SEM; 7 ultramicrotomes?2 with cryo attachments; plus ancillary specimen preparation equipment.
Job descriptions available at: https://www.hr.duke.edu/ Click ?Jobs? Under ?Job Descriptions? click ?Duke University Health System? Under ?Browse by Job Title? click ?M? At the bottom click ?Molecular Tech II, III, or IV? (Alternatively, to get to here, copy and paste: https://www.hr.duke.edu/jobs/descr_duhs/job_title.php?ID=M
These descriptions are generic, and not all jobs described within (e.g., histology) are required of the EM position.
EM Laboratory web site: http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain
Send resume to: Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710
A relatively new person in our EHS dept has informed us that UA is a strong gamma emitter and should be stored and disposed of in a stainless steel containersAND used in a stainless steel hood (or with other proper protection measures). This was a surprise to us as all former EHS staff have told us that though it DOES need to be disposed of with other radioactive waste, it can be used in a normal hood. We obviously want to be as safe as possible.
Can you advise on any special handling procedures used for UA?
Many thanks in advance!
danielle
Buck Institute for Age Research
==============================Original Headers============================== 3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 15:31:35 2009 3, 22 -- Received: from inverness.buckcenter.org (webmail.buckinstitute.org [64.84.58.24]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07LVZR8002117 3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan 2009 15:31:35 -0600 3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 22 -- Content-class: urn:content-classes:message 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: text/plain; 3, 22 -- charset="US-ASCII" 3, 22 -- Subject: RE: Uranyl Acetate handling, storage, and disposal 3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800 3, 22 -- Message-ID: {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org} 3, 22 -- In-Reply-To: {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} 3, 22 -- X-MS-Has-Attach: 3, 22 -- X-MS-TNEF-Correlator: 3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, and disposal 3, 22 -- Thread-Index: AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA== 3, 22 -- References: {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} 3, 22 -- From: "Danielle Crippen" {dcrippen-at-buckinstitute.org} 3, 22 -- To: {Microscopy-at-Microscopy.Com} 3, 22 -- Content-Transfer-Encoding: 8bit 3, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n07LVZR8002117 ==============================End of - Headers==============================
I'm curious - has this new EHS person done actual measurements (comparing distance from source, shielding, etc.) on the UA that is commonly used in EM labs, or are they assuming that you have undepleted UA? Maybe I'm mistaken, but I thought the UA we buy from the EM supply companies is not as radioactive as "plain" UA. I know I've tried to get counts off of it (with a gamma detector) & it isn't very hot.
Tamara
On Wed, 7 Jan 2009 15:32:23 -0600 dcrippen-at-buckinstitute.org wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear List, } } A relatively new person in our EHS dept has informed us } that UA is a } strong gamma emitter and should be stored and disposed } of in a stainless } steel containersAND used in a stainless steel hood (or } with other proper } protection measures). This was a surprise to us as all } former EHS staff } have told us that though it DOES need to be disposed of } with other } radioactive waste, it can be used in a normal hood. We } obviously want } to be as safe as possible. } } Can you advise on any special handling procedures used } for UA? } } Many thanks in advance! } } danielle } } Buck Institute for Age Research } } } } ==============================Original } Headers============================== } 3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 } 15:31:35 2009 } 3, 22 -- Received: from inverness.buckcenter.org } (webmail.buckinstitute.org [64.84.58.24]) } 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n07LVZR8002117 } 3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan } 2009 15:31:35 -0600 } 3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 3, 22 -- Content-class: urn:content-classes:message } 3, 22 -- MIME-Version: 1.0 } 3, 22 -- Content-Type: text/plain; } 3, 22 -- charset="US-ASCII" } 3, 22 -- Subject: RE: Uranyl Acetate handling, storage, } and disposal } 3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800 } 3, 22 -- Message-ID: } {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org} } 3, 22 -- In-Reply-To: } {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} } 3, 22 -- X-MS-Has-Attach: } 3, 22 -- X-MS-TNEF-Correlator: } 3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, } and disposal } 3, 22 -- Thread-Index: } AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA== } 3, 22 -- References: } {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org} } {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org} } {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} } 3, 22 -- From: "Danielle Crippen" } {dcrippen-at-buckinstitute.org} } 3, 22 -- To: {Microscopy-at-Microscopy.Com} } 3, 22 -- Content-Transfer-Encoding: 8bit } 3, 22 -- X-MIME-Autoconverted: from quoted-printable to } 8bit by ns.microscopy.com id n07LVZR8002117 } ==============================End of - } Headers==============================
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Email: reganhll-at-gmail.com Name: JOhnson
Organization: URI
Title-Subject: [Filtered] EM TOMOGRAPHY
Question: I am looking for a book on basics of EM tomograpyh. Though i am into EM since last 5yrs ...Need to know about EM tomography.
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Email: abesenyo-at-ibilabs.com Name: Alex Besenyo PhD
Organization: President
Title-Subject: [Filtered] Uranyl Acetate a Alpha Emitter
Question: There is a posting that needs clarification.
As the sole world wide manufacturer of Uranyl Acetate and other uranium compounds let me assure everyone that Uranyl Acetate is a alpha emitter and not a gamma emmiter.
As for storage good house keeping rules apply.
If any one has any direct questions regarding this they can post or contact me directly.
I worked with Uranium at ICN Pharmaceuticals and Isotope Products Labs making standards. Uranium is an alpha emitter, which is far more deadly internally. Also, Uranium is chemically toxic. So, keep it safe.
I doubt you'll find much dose coming off a small vial of U salt. Secondary x-rays may be produced from the alpha-particles fluorescing the surrounding matrix. Minimal shielding should suffice.
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: dcrippen-at-buckinstitute.org [mailto:dcrippen-at-buckinstitute.org] Sent: Wednesday, January 07, 2009 1:37 PM To: dkloos-at-parallaxray.com
Dear List,
A relatively new person in our EHS dept has informed us that UA is a strong gamma emitter and should be stored and disposed of in a stainless steel containersAND used in a stainless steel hood (or with other proper protection measures). This was a surprise to us as all former EHS staff have told us that though it DOES need to be disposed of with other radioactive waste, it can be used in a normal hood. We obviously want to be as safe as possible.
Can you advise on any special handling procedures used for UA?
Many thanks in advance!
danielle
Buck Institute for Age Research
==============================Original Headers============================== 3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 15:31:35 2009 3, 22 -- Received: from inverness.buckcenter.org (webmail.buckinstitute.org [64.84.58.24]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07LVZR8002117 3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan 2009 15:31:35 -0600 3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 22 -- Content-class: urn:content-classes:message 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: text/plain; 3, 22 -- charset="US-ASCII" 3, 22 -- Subject: RE: Uranyl Acetate handling, storage, and disposal 3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800 3, 22 -- Message-ID: {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org} 3, 22 -- In-Reply-To: {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} 3, 22 -- X-MS-Has-Attach: 3, 22 -- X-MS-TNEF-Correlator: 3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, and disposal 3, 22 -- Thread-Index: AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA== 3, 22 -- References: {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} 3, 22 -- From: "Danielle Crippen" {dcrippen-at-buckinstitute.org} 3, 22 -- To: {Microscopy-at-Microscopy.Com} 3, 22 -- Content-Transfer-Encoding: 8bit 3, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n07LVZR8002117 ==============================End of - Headers==============================
==============================Original Headers============================== 14, 30 -- From dkloos-at-parallaxray.com Wed Jan 7 17:51:53 2009 14, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) 14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07Nprki026992 14, 30 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 17:51:53 -0600 14, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) 14, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) 14, 30 -- (envelope-from {dkloos-at-parallaxray.com} ) 14, 30 -- id 1LKiBm-0000gH-5m; Wed, 07 Jan 2009 16:51:54 -0700 14, 30 -- Reply-To: {dkloos-at-parallaxray.com} 14, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com} 14, 30 -- To: {dcrippen-at-buckinstitute.org} 14, 30 -- Cc: {microscopy-at-microscopy.com} 14, 30 -- References: {200901072136.n07Laq35015255-at-ns.microscopy.com} 14, 30 -- Subject: RE: [Microscopy] RE: Uranyl Acetate handling, storage, and disposal 14, 30 -- Date: Wed, 7 Jan 2009 15:51:48 -0800 14, 30 -- Organization: Parallax Research 14, 30 -- Message-ID: {6735FBA29934490C8F3B6EB1137FF376-at-donl} 14, 30 -- MIME-Version: 1.0 14, 30 -- Content-Type: text/plain; 14, 30 -- charset="us-ascii" 14, 30 -- Content-Transfer-Encoding: 7bit 14, 30 -- X-Mailer: Microsoft Office Outlook 11 14, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 14, 30 -- In-Reply-To: {200901072136.n07Laq35015255-at-ns.microscopy.com} 14, 30 -- thread-index: AclxEA7jOgj41WzYT5aTp4V8xWeubQAEkOvQ 14, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 14, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com 14, 30 -- X-AntiAbuse: Original Domain - microscopy.com 14, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] 14, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com ==============================End of - Headers==============================
Dear Listers, Today we have encountered what appears to be defective Kodak SO-163 electron image film, 3 1/4 x 4 inch, catalog #8010100. Those of you who use this film will know that when loading the film into cassettes, if the notch in the film is at the upper right-hand corner, then the emulsion side of the film is up. The suspected defective film has the emulsion side down with the notch at the upper right.
In addition, normally these packets of film have a slight upward curl (concave) with the emulsion side up and notch to the upper right. This potentially defective film has a downward curl (convex) with the notch to the upper right.
On the box of film which is a multipak of 250 sheets, the following numbers are found on the label:
Emul No. 239 002 07 2010-08 00277396
We do not know how widespread this problem is but we recommend not to use film of this lot number until more information from Kodak is available.
Mr. Donald Gantz Research Histologist/Electron Microscopist Dept. Physiology and Biophysics Center for Advanced Biomedical Research Boston University School of Medicine 700 Albany Street Boston, MA 02118 email: gantz-at-bu.edu phone: 617-638-4017
==============================Original Headers============================== 7, 25 -- From gantz-at-bu.edu Wed Jan 7 18:10:14 2009 7, 25 -- Received: from relay15.bu.edu (relay15.bu.edu [128.197.27.66]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n080AECu008276 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 18:10:14 -0600 7, 25 -- X-Envelope-From: gantz-at-bu.edu 7, 25 -- Received: from biophysics1.bumc.bu.edu (biophysics1.bumc.bu.edu [155.41.208.134]) 7, 25 -- by relay15.bu.edu (8.13.1/8.13.1) with ESMTP id n0809mVA013635; 7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500 7, 25 -- Received: from gantz (cabr3-dhcp-208-153.bumc.bu.edu [155.41.208.153]) 7, 25 -- by biophysics1.bumc.bu.edu (8.13.8/8.13.8) with SMTP id n0809mF4015021; 7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500 7, 25 -- Message-ID: {000b01c97125$69b0c190$99d0299b-at-gantz} 7, 25 -- From: "Don Gantz" {gantz-at-bu.edu} 7, 25 -- To: {Microscopy-at-microscopy.com} 7, 25 -- Cc: {bullitt-at-bu.edu} 7, 25 -- Subject: Defective Electron Image Film 7, 25 -- Date: Wed, 7 Jan 2009 19:09:45 -0500 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="iso-8859-1" 7, 25 -- Content-Transfer-Encoding: 7bit 7, 25 -- X-Priority: 3 7, 25 -- X-MSMail-Priority: Normal 7, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807 7, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
Uranium is a natural radioactive element which is "mainly" alpha emitter. Uranium isotopes also have a very small probablity of "spontaneous fission", as well (this is not the main concern here of course).
Since, the decay product of Uranium is also radioactive, you will need to follow the decay tree to get a better feeling of what kinds of other elements/particles might be present in Uranyl Acetate.
You may be able to construct the decay tree using the information from
http://atom.kaeri.re.kr/ton/nuc7.html
It will be a tedious calculation to assess the health risk from handling Uranyl Acetate. It is always best to opt on the side of more shielding. Go with ALARA (As Low As Reasonably Achievable) principles.
Hope this helps, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Wed, Jan 7, 2009 at 11:36 PM, {dcrippen-at-buckinstitute.org} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear List, } } A relatively new person in our EHS dept has informed us that UA is a } strong gamma emitter and should be stored and disposed of in a stainless } steel containersAND used in a stainless steel hood (or with other proper } protection measures). This was a surprise to us as all former EHS staff } have told us that though it DOES need to be disposed of with other } radioactive waste, it can be used in a normal hood. We obviously want } to be as safe as possible. } } Can you advise on any special handling procedures used for UA? } } Many thanks in advance! } } danielle } } Buck Institute for Age Research } } } } ==============================Original Headers============================== } 3, 22 -- From dcrippen-at-buckinstitute.org Wed Jan 7 15:31:35 2009 } 3, 22 -- Received: from inverness.buckcenter.org (webmail.buckinstitute.org [64.84.58.24]) } 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n07LVZR8002117 } 3, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Jan 2009 15:31:35 -0600 } 3, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 3, 22 -- Content-class: urn:content-classes:message } 3, 22 -- MIME-Version: 1.0 } 3, 22 -- Content-Type: text/plain; } 3, 22 -- charset="US-ASCII" } 3, 22 -- Subject: RE: Uranyl Acetate handling, storage, and disposal } 3, 22 -- Date: Wed, 7 Jan 2009 13:31:34 -0800 } 3, 22 -- Message-ID: {69142A827D247F45877B305E991DB61F18DD61-at-inverness.buckcenter.org} } 3, 22 -- In-Reply-To: {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} } 3, 22 -- X-MS-Has-Attach: } 3, 22 -- X-MS-TNEF-Correlator: } 3, 22 -- Thread-Topic: Uranyl Acetate handling, storage, and disposal } 3, 22 -- Thread-Index: AclxDrE0qGF/HDu+TY2cyGuAAHfcOgAABlUgAAAIS2AAABVbgA== } 3, 22 -- References: {69142A827D247F45877B305E991DB61F18DD5E-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD5F-at-inverness.buckcenter.org} {69142A827D247F45877B305E991DB61F18DD60-at-inverness.buckcenter.org} } 3, 22 -- From: "Danielle Crippen" {dcrippen-at-buckinstitute.org} } 3, 22 -- To: {Microscopy-at-Microscopy.Com} } 3, 22 -- Content-Transfer-Encoding: 8bit } 3, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n07LVZR8002117 } ==============================End of - Headers============================== }
I've included a group of emails (not all) from September, 1998 on this same discussion. We did measure beta and gamma emissions greater than background levels from the uranyl acetate stock reagent bottles, not from solutions.
It's still wise to use proper PPE methods especially when weighing and preparing solutions. Store the reagent bottles inside/behind shielding in a relatively secluded area and treat old solutions as heavy metal waste. Don't pour down sinks!
Bruce F. Ingber USDA-ARS, SRRC Biologist/Electron Microscopy 1100 Robert E. Lee Blvd. New Orleans, LA 70124 Bruce.Ingber-at-ars.usda.gov
ph. 504-286-4270 fax 504-286-4217 cel 504-782-6323
-----Original Message----- X-from: thoward-at-unm.edu [mailto:thoward-at-unm.edu] Sent: Wednesday, January 07, 2009 5:21 PM To: Ingber, Bruce
I'm curious - has this new EHS person done actual measurements (comparing distance from source, shielding, etc.) on the UA that is commonly used in EM labs, or are they assuming that you have undepleted UA? Maybe I'm mistaken, but I thought the UA we buy from the EM supply companies is not as radioactive as "plain" UA. I know I've tried to get counts off of it (with a gamma detector) & it isn't very hot.
Tamara
On Wed, 7 Jan 2009 15:32:23 -0600 dcrippen-at-buckinstitute.org wrote: } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Dear List, } } A relatively new person in our EHS dept has informed us } that UA is a } strong gamma emitter and should be stored and disposed } of in a stainless } steel containersAND used in a stainless steel hood (or } with other proper } protection measures). This was a surprise to us as all } former EHS staff } have told us that though it DOES need to be disposed of } with other } radioactive waste, it can be used in a normal hood. We } obviously want } to be as safe as possible. } } Can you advise on any special handling procedures used } for UA? } } Many thanks in advance! } } danielle } } Buck Institute for Age Research -----Original Message----- X-from: Warren E Straszheim [mailto:bingber.BAYOU.SRRCDOM] Sent: Wednesday, September 23, 1998 4:13 PM To: bingber.BAYOU.SRRCDOM
FWIW
Some years ago I heard an account of a radiation safety inspection as part of a larger safety review at a large lab. The lab was involved in coal research, as were we, and had a fair amount of coal samples stored in glass jars. An inspection team came thru and happened to check the jars for radiation, found some, and instructed the researchers that they would need to start following radiation safety procedures.
Now coal can contain minute amounts of uranium in its mineral matter, but not enough that should set off a detector. Besides the detectors were setup to measure alpha particles, and there was no way that alpha particles emitted from the contents of a glass jar should be detected on the outside.
A little digging revealed that there was in fact a little radiation present, but it was a slight residue left on the jar surface after washing. Apparently the jars went through the same washer as did other jars which had held radioactive materials. I think the radiation safety folks may have received additional training after the incident.
} Anyway, while gathering all uranium compounds, our radiation safety officer checked the compounds using a dosimeter that recorded alpha, beta, and gamma radiation. Levels of radiation were found that were just below OSHA guidelines for maximum daily exposure when the powder was checked from several inches away from the dosimeter. Thus, we decided to store the compounds in an acrylic box behind a beta shield in an isolated location. Whenever the actual Uranyl acetate is weighed to prepare dilute solutions proper safety precautions are recommended, i.e. use a beta shield, wear gloves and dust mask, weigh in a low occupancy room, etc. } } We repeated our dosimeter readings last month with two different alpha, beta, and gamma dosimeters taking readings several feet from the bottles, removing the two acrylic shields one by one, and then with the cover of the UAc exposed (always moving closer to the chemical source). I won't enter the millirem/rad discussion; suffice to say it's an interesting little experiment for your support staff especially with the dosimeters left on audio signal.
{snip}
---------------------------------------------------- Warren E. Straszheim 23 Town Engineering Iowa State University Ames IA, 50011-3232
electron microscopy, x-ray analysis, image analysis, computer applications
-----Original Message----- X-from: "Woody.N.White-at-mcdermott.com"-at-Sparc5.Microscopy.Com [mailto:bingber.BAYOU.SRRCDOM] Sent: Thursday, September 17, 1998 9:28 PM To: bingber.BAYOU.SRRCDOM
This bounced once, I shall try again... (Nestor ignore the (not spam) email if this makes it to the listserver ok) ----------------------------------------------------------------
Well....
The Nuclear Regulatory Commission limits of skin & extremity (hands, feet) is 50 Rem per year. ...Not something to "shoot" for,
since the dose is also limited to "As Low As Reasonably Achievable".
The (damage) conversion coefficient from mR from this source (no alpha if not ingested) to mRem is ~1. 50 Rem = 50,000 mRem. At a
dose rate of 0.6 mR/hr, one would have to hold the container for many years to receive a one year maximum dose (50,000 / 0.6 per hr = max hours exposure). At 5 mR/hr, it would be 50,000 / 5. At that
one would have to hold the container for 10,000 hours before exceeding NRC dose limits. Exposure will also decrease as a function of the square of the distance from the source.
For medical tests to discover any changes in body chemistry, it would take about 50 Rem acute whole body exposure.
Less dose is always better, but in realistic terms the dose from the UA should not be of any concern. If this level is of concern, do not fly in airplanes, live at high elevations, avoid all medical
radiation, avoid certain beaches, beware of granite buildings, run from radium dial watches, etc. :)
The real danger is if the UA enters the body where the alpha source
is in direct contact with livings tissue. Radiological bio-assay (urine/fecal) would be required to detect this.
Woody White McDermott Technology
-----Original Message----- X-from: William Tivol [mailto:bingber.BAYOU.SRRCDOM] Sent: Thursday, September 17, 1998 9:58 PM To: bingber.BAYOU.SRRCDOM
Dear David,
} Using Bill's number's, you would } still be well under the limits for occupational exposure if you were in } constant contact with .6 millirem/hr for a 2000 hr work year, (correct me, } but my references place the limits at 1.25 rem/quarter, 5 rem/year whole } body
These limits are for radiation workers. Because we get paid, we can be exposed to a greater risk. The limit for the general population is 0.5 rem/year whole body, and I think this limit also applies to women who are or may be pregnant. I do not know the status of graduate students; I'd be inclined to err on the side of caution--especially since it is fairly easy to keep exposure to UA low. Yours, Bill Tivol -----Original Message----- X-from: David Bentley [mailto:bingber.BAYOU.SRRCDOM] Sent: Thursday, September 17, 1998 8:09 PM To: bingber.BAYOU.SRRCDOM
Responding with tidbits regarding this thread.
We make up a saturated stock bottle which we draw from, and replenish with UA and water (8-10g UA/100 ml) from time to time. The insoluble material is described in the Merck Index as being due to insoluble basic salts. It describes Uranyl Acetate as being "freely soluble in water acidulated with acetic acid" For years, we have followed a modification of a procedure from Millonig's 1976 book Laboratory Manual of Biological Electron Microscopy (pg 53) and added a few drops of acetic acid per 100mls of stock saturated UA (stored in a brown bottle). This seems to push the ppt reaction the other way and give a clear solution. There seems to be little difference in staining as long as only a few drops of acetic acid are used. Changing the pH of the stain by much, is risky though as there are numerous papers and procedures which modify the effects of UA stain by doing so. We have raised the pH to the 4.5-5.5 (any higher and the UA will ppt) and gotten improved staining but with unacceptable amounts of ppt on the sections.
When compared with the other chemicals in the EM lab, UA would seem to be relatively safe when used carefully. Ingestion and inhalation (exposure to dust) are our major concern due to heavy metal toxicity as well as the radiation hazards. Making sure that surfaces are not contaminated, and cleaning any spillage immediately from bottles and tables before it dries are important steps. Wearing gloves, and hand washing after glove removal are also important safeguards.
The radiation exposure hazard under most operating conditions seems minimal. The least exposure possible is desirable (ALARA), when you don't need to handle it, don't be near it. Using Bill's number's, you would still be well under the limits for occupational exposure if you were in constant contact with .6 millirem/hr for a 2000 hr work year, (correct me, but my references place the limits at 1.25 rem/quarter, 5 rem/year whole body and 18.75 rem/quarter, 75 rem/year for extremities (Rayburn)) The other factor to keep in mind is that we are not talking about a whole body exposure, but just exposure to the hands. All in all, the amount of exposure while making up and staining grids seems miniscule. -----Original Message----- X-from: ROBIN CROSS [mailto:bingber.BAYOU.SRRCDOM] Sent: Wednesday, September 16, 1998 7:10 AM To: bingber.BAYOU.SRRCDOM
The concentration quoted is far higher (btw at what concentration in water does UA become a saturated solution?) than normally would be used for EM staining.
} A solution of 10g UA in 15mls H2O was measured with a Geiger counter.
I have been using UA for many years and I recall that whenever I questioned and investigated its possible radiation implications I have been assured that it is not dangerous at the concentrations and quantities we use, provided that it is not ingested. Robin H Cross Director : EM Unit, Rhodes University, Grahamstown, South Africa eurc-at-giraffe.ru.ac.za - tel: +27 46 603 8168 - fax: +27 46 622 4377 http://www.ru.ac.za/affiliates/emu/em.htm
As an aside, the pretty flowered dinnerware from the 50's, the vivid oranges and yellows are from uranium. If you have any, run a Geiger counter over them, you'll be surprised the number of counts. Also the mantles from gas and propane lanterns contain radioactive thorium. In the past health physicist have suggested using them(sealed in their bags) for check sources for counters.
Regardless, because of the toxicity, radiation hazard, as well as expenses to purchase(well over $1.00/gm) and dispose of UA, minimizing the amount needed to be discarded and wasted seems desirable. To the extents possible, use of minimal amounts, and if considerable staining is done, making stock saturated solutions which can be diluted to the desired concentration as needed, are good ways to conserve UA, minimize radiation exposure, and inhalation and ingestion hazards.
Now, if we are starting a poll for the chemicals in the EM Lab that make us the most anxious, my vote is for cacodylate. -----Original Message----- X-from: William Tivol [mailto:bingber.BAYOU.SRRCDOM] Sent: Wednesday, September 16, 1998 4:34 PM To: bingber.BAYOU.SRRCDOM
Dear Josephine, } } A solution of 10g UA in 15mls H2O was measured with a Geiger counter. } 500 } counts/sec was generated. } A supplier had measured 100g UA :- } 1 Alpha - {2 counts/sec, using a 540 scintillation meter with AP-2 Probe } 2 Beta - } 500 counts/sec, using a 540 E1 probe coupled to a GM Meter (this } determines beta events and some low energy gamma events) } 3 Gamma dose Rate (energy field) - two measurements done: } using Mini monitor type R with GM Probe - 0.6mR/hr (mainly gamma) } and Ionization chamber DMM 95/0500 - 5 mR/hr (Beta and Gamma energy } field). } 4 Specific Activity (U approx. 55%) = 1.04 x 10 { {...} } Bq { {...} } gm
} { {...} } . } I calculated approximately the expected activity from 30 g UA (about 0.1 mole, or 6*10^22 atoms). T_1/2 is 4.4*10^9 y and there are 3.1*10^7 s/y, so the decay rate is 5*10^-18 s^-1, and the activity is 3*10^5 Bq. The build-up of daughter products with shorter half-lives will reach steady state at which point the activities of the daughters will be the same as that of the parent. Pa 234 has a gamma transition, and there are several betas in the chain. The longer-lived isotopes in the chain have lives of 10^4 to 10^5 y, and these will not be at steady state (unless your UA is *very* old ;-) ). 0.6 mR/hr is a significant amount of exposure, and, if one were to hold the jars for some minutes, a sizable fraction of the allowed annual dose would be attained.
} Can UA be used openly without protection in laboratory? } Small amounts can be used, but be sure to wash hands before eating. One area of the lab should be used for UA. A quiet area with little traffic is best. UA, while not nearly the most dangerous EM reagent, should still be treated with respect. Yours, Bill Tivol
==============================Original Headers============================== 63, 31 -- From Bruce.Ingber-at-ARS.USDA.GOV Thu Jan 8 09:50:59 2009 63, 31 -- Received: from messagescreen3.ars.usda.gov (messagescreen3.ars.usda.gov [199.133.180.150]) 63, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n08FowRF024837 63, 31 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 09:50:58 -0600 63, 31 -- Received: from CO-MAILBH-02.ARSNET.ARS.USDA.GOV (ars.usda.gov [199.133.183.227] (may be forged)) 63, 31 -- by messagescreen3.ars.usda.gov (8.13.8/8.13.8) with ESMTP id n08FobJw024001 63, 31 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 09:50:58 -0600 63, 31 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 63, 31 -- Thu, 8 Jan 2009 08:48:55 -0700 63, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 63, 31 -- Content-class: urn:content-classes:message 63, 31 -- MIME-Version: 1.0 63, 31 -- Content-Type: text/plain; 63, 31 -- charset="us-ascii" 63, 31 -- Subject: RE: [Microscopy] Uranyl Acetate handling, storage, and 63, 31 -- Date: Thu, 8 Jan 2009 08:48:55 -0700 63, 31 -- Message-ID: {8017F94146BF634DA9414E4B9088525B03E8D4D4-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 63, 31 -- X-MS-Has-Attach: 63, 31 -- X-MS-TNEF-Correlator: 63, 31 -- Thread-Topic: [Microscopy] Uranyl Acetate handling, storage, and 63, 31 -- Thread-Index: AclxHrQEWtl0gXUIQCyvDFYeWl60ygAg4LogAAGC4gA= 63, 31 -- References: {200901072321.n07NLLKA030038-at-ns.microscopy.com} 63, 31 -- From: "Ingber, Bruce" {Bruce.Ingber-at-ARS.USDA.GOV} 63, 31 -- To: {microscopy-at-microscopy.com} 63, 31 -- X-OriginalArrivalTime: 08 Jan 2009 15:48:55.0477 (UTC) FILETIME=[9CBBD650:01C971A8] 63, 31 -- X-MessageScreenMessageID: 1231429858.626943.1253.994197512 63, 31 -- X-MessageScreenContentScore: Score of 5 assigned to Content 63, 31 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 63, 31 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 63, 31 -- Content-Transfer-Encoding: 8bit 63, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n08FowRF024837 ==============================End of - Headers==============================
Dear Mr. Gantz, I have received boxes of Kodak 4489 film in the past that were marked wrong. It is obvious in the darkroom when you are loading the film in the holders that the emulsion was on the wrong side (or, alternatively, that they put the notch in the wrong place). The emulsion is the brighter side, the back is the dark side. This happened with two or three boxes, several years ago, and I informed the Kodak company with their reply card that comes with the film. I received no reply. I used the film, with the emulsion side up, as usual and it was just fine. The emulsion makes the plastic curl slightly towards it, but it usually flattens out after processing. I don't think the film is faulty, just marked wrong. Maybe test a few sheets first, then use as usual. Regards,
-----Original Message----- X-from: gantz-at-bu.edu [mailto:gantz-at-bu.edu] Sent: January 7, 2009 4:14 PM To: maryflet-at-interchange.ubc.ca
Dear Listers, Today we have encountered what appears to be defective Kodak SO-163 electron image film, 3 1/4 x 4 inch, catalog #8010100. Those of you who use this film will know that when loading the film into cassettes, if the notch in the film is at the upper right-hand corner, then the emulsion side of the film is up. The suspected defective film has the emulsion side down with the notch at the upper right.
In addition, normally these packets of film have a slight upward curl (concave) with the emulsion side up and notch to the upper right. This potentially defective film has a downward curl (convex) with the notch to the upper right.
On the box of film which is a multipak of 250 sheets, the following numbers are found on the label:
Emul No. 239 002 07 2010-08 00277396
We do not know how widespread this problem is but we recommend not to use film of this lot number until more information from Kodak is available.
Mr. Donald Gantz Research Histologist/Electron Microscopist Dept. Physiology and Biophysics Center for Advanced Biomedical Research Boston University School of Medicine 700 Albany Street Boston, MA 02118 email: gantz-at-bu.edu phone: 617-638-4017
==============================Original Headers============================== 7, 25 -- From gantz-at-bu.edu Wed Jan 7 18:10:14 2009 7, 25 -- Received: from relay15.bu.edu (relay15.bu.edu [128.197.27.66]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n080AECu008276 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Jan 2009 18:10:14 -0600 7, 25 -- X-Envelope-From: gantz-at-bu.edu 7, 25 -- Received: from biophysics1.bumc.bu.edu (biophysics1.bumc.bu.edu [155.41.208.134]) 7, 25 -- by relay15.bu.edu (8.13.1/8.13.1) with ESMTP id n0809mVA013635; 7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500 7, 25 -- Received: from gantz (cabr3-dhcp-208-153.bumc.bu.edu [155.41.208.153]) 7, 25 -- by biophysics1.bumc.bu.edu (8.13.8/8.13.8) with SMTP id n0809mF4015021; 7, 25 -- Wed, 7 Jan 2009 19:09:48 -0500 7, 25 -- Message-ID: {000b01c97125$69b0c190$99d0299b-at-gantz} 7, 25 -- From: "Don Gantz" {gantz-at-bu.edu} 7, 25 -- To: {Microscopy-at-microscopy.com} 7, 25 -- Cc: {bullitt-at-bu.edu} 7, 25 -- Subject: Defective Electron Image Film 7, 25 -- Date: Wed, 7 Jan 2009 19:09:45 -0500 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="iso-8859-1" 7, 25 -- Content-Transfer-Encoding: 7bit 7, 25 -- X-Priority: 3 7, 25 -- X-MSMail-Priority: Normal 7, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807 7, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 32 -- From maryflet-at-interchange.ubc.ca Thu Jan 8 12:41:25 2009 16, 32 -- Received: from mr4.mail-relay.ubc.ca (mr4.mail-relay.ubc.ca [137.82.45.7]) 16, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n08IfOq3010949 16, 32 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 12:41:25 -0600 16, 32 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 16, 32 -- by mr4.mail-relay.ubc.ca (Postfix) with ESMTP id E6A591B276 16, 32 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 10:41:23 -0800 (PST) 16, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 16, 32 -- by smtp.interchange.ubc.ca 16, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 16, 32 -- with ESMTP id {0KD60098018Y29-at-smtp.interchange.ubc.ca} for 16, 32 -- microscopy-at-microscopy.com; Thu, 08 Jan 2009 10:41:23 -0800 (PST) 16, 32 -- Date: Thu, 08 Jan 2009 10:40:41 -0800 16, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 16, 32 -- Subject: RE: [Microscopy] Defective Electron Image Film 16, 32 -- In-reply-to: {200901080013.n080DvWV017600-at-ns.microscopy.com} 16, 32 -- To: gantz-at-bu.edu 16, 32 -- Cc: microscopy-at-microscopy.com 16, 32 -- Reply-to: maryflet-at-interchange.ubc.ca 16, 32 -- Message-id: {0KD60098118Y29-at-smtp.interchange.ubc.ca} 16, 32 -- Organization: Materials Eng. 16, 32 -- MIME-version: 1.0 16, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 16, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 32 -- Content-type: text/plain; charset=us-ascii 16, 32 -- Content-transfer-encoding: 7bit 16, 32 -- Thread-index: AclxJgCge6cB0wedSUSS/HPlI4ibvQAlN+xg 16, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.1.8.182828 16, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 16, 32 -- X-PerlMx-Spam: Probability=8%, Report=BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, ECARD_WORD 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 16, 32 -- X-Spam-Level: 16, 32 -- X-Spam-Flag: No ==============================End of - Headers==============================
We are doing a very quick survey about preferences for computer platforms used for digital imaging microscopy. There are only 4 questions (none of them nasty multi-part!) so will take less than a minute to click on your choices.
Your input will help us provide the camera systems you need to take your work to the next level, so thank you!!
http://www.zoomerang.com/Survey/?p=WEB228P5UWUTCB
--David Hitrys
==============================Original Headers============================== 7, 25 -- From dhitrys-at-qimaging.com Thu Jan 8 16:44:06 2009 7, 25 -- Received: from smtp02.lnh.mail.rcn.net (smtp02.lnh.mail.rcn.net [207.172.157.102]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n08Mi6XH008842 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 16:44:06 -0600 7, 25 -- Received: from mr08.lnh.mail.rcn.net ([207.172.157.28]) 7, 25 -- by smtp02.lnh.mail.rcn.net with ESMTP; 08 Jan 2009 17:44:06 -0500 7, 25 -- Received: from smtp01.lnh.mail.rcn.net (smtp01.lnh.mail.rcn.net [207.172.4.11]) 7, 25 -- by mr08.lnh.mail.rcn.net (MOS 3.10.4-GA) 7, 25 -- with ESMTP id KOD15676; 7, 25 -- Thu, 8 Jan 2009 17:43:44 -0500 (EST) 7, 25 -- Received: from 209-6-255-165.c3-0.frm-ubr2.sbo-frm.ma.cable.rcn.com (HELO DavidM1330) ([209.6.255.165]) 7, 25 -- by smtp01.lnh.mail.rcn.net with ESMTP; 08 Jan 2009 17:43:44 -0500 7, 25 -- From: "David Hitrys" {dhitrys-at-qimaging.com} 7, 25 -- To: {microscopy-at-microscopy.com} 7, 25 -- Subject: PC or Mac preference for Digital Microscopy (4-question survey) 7, 25 -- Date: Thu, 8 Jan 2009 17:43:29 -0500 7, 25 -- Message-ID: {013401c971e2$872f6e70$958e4b50$-at-com} 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="iso-8859-1" 7, 25 -- X-Mailer: Microsoft Office Outlook 12.0 7, 25 -- Thread-Index: Aclx4n3tSxv5TJkiSJePgnsd+7BkFA== 7, 25 -- Content-Language: en-us 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n08Mi6XH008842 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abesenyo-at-ibilabs.com Name: Alex Besenyo PhD
Organization: ibilabs
Title-Subject: [Filtered] uranyl compounds are alpha emitters only
Question: Question:
Is it true that the stuff we use has been somehow depleted, so that it isn't as radioactive as "real" uranyl salts? Or is this yet another old wive's tale of EM?!
Reply:
When we manufacture these compounds we purchase the raw uranium in a depleted state from the government. There is no chance for error here. We do not use natural uranium.
This means that the enrichable uranium U-235 has been removed. The then U-238 which only emitts alpha radiation is procesed.
The term "depleted" means that U-235 has been removed.
If even by the slightest chance that U235 were present then every alarm would go off in our facility because Beta and Gamma radiation is detected.
I hope this answers everybodies concerns.
Our products are sold exclusively through a distributor network and all of them have been instructed on this information.
I only responded when I saw the original post and I had to respond before it got out of control.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alerch-at-mcw.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: alerch-at-mcw.edu Name: Alexandra Lerch-Gaggl
Organization: Medical College of Wisconsin
Title-Subject: [Filtered] How to find companies that buy/salvage older equipment
Question: We have a Biorad MRC 600 confocal microscope, where we had the galvanos replaced a while ago. Now it seems that the scanner has the same problem again. Is there a way that I could find companies or institutions that salvage such equipment? Thank you.
Did you have a chance to check the information from
http://atom.kaeri.re.kr/ton/nuc7.html
Let me construct the decay tree. I'm sure everybody in this list can do it but, you keep insisting that "uranyl compounds are alpha emitters only" so, I will take the time to do the job and post in to the list.
Let's start with U-238 which is the starting element in your compound.
1) U-238 decays into Th-234 by Alpha decay
2) Th-234 decays into Pa-234 by Beta decay
3) Pa-234 decays into U-234 by Beta decay
4) U-234 decays into Th-230 by Alpha decay
5) Th-230 decays into Ra-226 by Alpha decay
6) Ra-226 decays into Rn-222 by Alpha decay
7) Rn-222 decays into Po-218 by Alpha decay
8) Po-218 decays into Pb-214 by Alpha decay
9) Pb-214 decays into Bi-214 by Beta decay
10) Bi-214 decays into Po-214 by Beta decay
11) Po-214 decays into Pb-210 by Alpha decay
12) Pb-210 decays into Bi-210 by Beta decay
13) Bi-210 decays into Po-210 by Beta decay
14) Po-210 decays into Pb-206 by Alpha decay
Pb-206 is STABLE so, it is the last element to be produced as a result of U-238 radioactive decay.
I have constructed the above decay tree using the information from http://atom.kaeri.re.kr/ton/nuc7.html While constructing the above decay tree I have used the branch which has the highest branch ratio (above 99% in each case).
I do not understand why you are trying to keep things "under control"?
By the way, I'm a Nuclear Engineer.
One does not even need to be nuclear engineer to understand this. Even in high school science classes people learn about radioactive decay series e.g. A decays into B and B decays into C...
Best, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Fri, Jan 9, 2009 at 2:27 AM, {abesenyo-at-ibilabs.com} wrote: } } Email: abesenyo-at-ibilabs.com } Name: Alex Besenyo PhD } } Organization: ibilabs } } Title-Subject: [Filtered] uranyl compounds are alpha emitters only } } Question: Question: } } Is it true that the stuff we use has been somehow } depleted, so that it isn't as radioactive as "real" uranyl } salts? Or is this yet another old wive's tale of EM?! } } Reply: } } When we manufacture these compounds we purchase the raw uranium in a } depleted state from the government. There is no chance for error } here. We do not use natural uranium. } } This means that the enrichable uranium U-235 has been removed. } The then U-238 which only emitts alpha radiation is procesed. } } The term "depleted" means that U-235 has been removed. } } If even by the slightest chance that U235 were present then every } alarm would go off in our facility because Beta and Gamma radiation } is detected. } } I hope this answers everybodies concerns. } } Our products are sold exclusively through a distributor network and } all of them have been instructed on this information. } } I only responded when I saw the original post and I had to respond } before it got out of control. } } Sincerely } Alex Besenyo PhD } } }
Your comments are informative but, to my personal taste, definitely a bit negative in tone for this list. Your "schooling" of Alex isn't really necessary and I think we could get your information in a more positive and collegial way.
Regarding the decay tree, I note from the provided link information that the half-life of U-238 is { {4.468E+9 years} } . It has been a while since my high school chemistry but I'm wondering how much Th-234 and associated beta emission danger we are really dealing with here; seems like there must be a very small amount of Th-234 produced with such a long half-life of the original U-238. Maybe you could comment on the danger of this.
Thanks,
Dale
celikaktas-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Alex, } } Did you have a chance to check the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } Let me construct the decay tree. I'm sure everybody in this list can } do it but, you keep insisting that "uranyl compounds are alpha } emitters only" so, I will take the time to do the job and post in to } the list. } } Let's start with U-238 which is the starting element in your compound. } } 1) U-238 decays into Th-234 by Alpha decay } } 2) Th-234 decays into Pa-234 by Beta decay } } 3) Pa-234 decays into U-234 by Beta decay } } 4) U-234 decays into Th-230 by Alpha decay } } 5) Th-230 decays into Ra-226 by Alpha decay } } 6) Ra-226 decays into Rn-222 by Alpha decay } } 7) Rn-222 decays into Po-218 by Alpha decay } } 8) Po-218 decays into Pb-214 by Alpha decay } } 9) Pb-214 decays into Bi-214 by Beta decay } } 10) Bi-214 decays into Po-214 by Beta decay } } 11) Po-214 decays into Pb-210 by Alpha decay } } 12) Pb-210 decays into Bi-210 by Beta decay } } 13) Bi-210 decays into Po-210 by Beta decay } } 14) Po-210 decays into Pb-206 by Alpha decay } } Pb-206 is STABLE so, it is the last element to be produced as a result } of U-238 radioactive decay. } } I have constructed the above decay tree using the information from } http://atom.kaeri.re.kr/ton/nuc7.html } While constructing the above decay tree I have used the branch which } has the highest branch ratio (above 99% in each case). } } I do not understand why you are trying to keep things "under control"? } } By the way, I'm a Nuclear Engineer. } } One does not even need to be nuclear engineer to understand this. Even } in high school science classes people learn about radioactive decay } series e.g. A decays into B and B decays into C... } } Best, } Ayten. }
==============================Original Headers============================== 7, 23 -- From dac-at-research.umass.edu Fri Jan 9 08:36:22 2009 7, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09EaMPb032435 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 08:36:22 -0600 7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 23 -- (authenticated bits=0) 7, 23 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n09EaLpI010451 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 09:36:22 -0500 7, 23 -- Message-ID: {49676117.7030802-at-research.umass.edu} 7, 23 -- Date: Fri, 09 Jan 2009 09:37:11 -0500 7, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 23 -- Reply-To: dac-at-research.umass.edu 7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 7, 23 -- MIME-Version: 1.0 7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 23 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters 7, 23 -- only 7, 23 -- References: {200901090743.n097ht8p015453-at-ns.microscopy.com} 7, 23 -- In-Reply-To: {200901090743.n097ht8p015453-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
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Title-Subject: [Filtered] cryo-TEM positions at Northwestern Univ.
Question: Two Open Positions Research Associate or Research Faculty Cryo-Electron Microscopy of Soft and Biological Structures Northwestern University, Evanston, IL Two research associate/research faculty positions are immediately available at Northwestern University in the area of analytical cryo- electron microscopy of soft and biological structures. Supported by the Keck Foundation, Chicago Biomedical Consortium and other federally funded research programs, the two positions are created to advance specimen preparation of biological structures for electron microscopy, especially oocytes, and the use of analytical TEM/STEM techniques to monitor bioelemental distribution across sub-cellular compartments at nanoscale spatial resolution. The research will principally employ a unique dual-EDS dedicated Hitachi cryo- STEM (based on HD-2300A platform) to be installed at Northwestern in summer 2009. In addition, the Northwestern University Atomic and Nanoscale Characterization Experimental (NUANCE) Center (www.nuance.northwestern.edu) and Quantitative Bioelement Imaging Center (QBIC) in the in the Chemistry of Life Processes Institute (http://www.clp.northwestern.edu/) have several SEMs/S/TEMs and complementary confocal, optical, scanning probe and laser-ablation mass spectrometry capabilities. The candidates are expected to develop cryo-specimen preparation protocols for oocytes and conduct analytical studies of elemental distribution in complex biological structures using STEM imaging and EDS analysis. The project is a part of extensive interdisciplinary collaborative initiatives among Professors Vinayak P. Dravid (www.northwestern.edu/vpdgroup), Tom OíHalloran (http://chemgroups.northwestern.edu/ohalloran/), Teresa Woodruff (http://www.northwestern.edu/neurobiology/faculty/Woodruff2/) and Jonathan Silverstein (http://home.uchicago.edu/~jcs/) to unravel the mysteries of nanoscale chemical architecture of Oocyte at various stages of fertilization. As a result, there are ample opportunities for personal and professional growth at the intersection of life and physical sciences, medicine and advanced instrumentation. The positions require a PhD in physical/biological sciences/engineering. Considerable experience in analytical and cryo-S/TEM is required and hands-on training in cryo-preparation techniques is highly desirable. Prior knowledge of imaging filter/spectral imaging and EELS is desirable but not mandatory. The positions are available immediately for at least two years, with the possibility for extension for additional period upon mutual agreement. Salary and compensation would commensurate with experience. Please forward curriculum vitae with three references to: Mr. Kim McCumber Email: ohalloran-ofc-at-northwestern.edu
I work in a place that does not permit the use of Uranyl salts for EM use because of the radiation dangers (and a healthy respect for ALARA), and am always trying to educate people about what these dangers are with facts. Thus I was very interested in this thread.
I did have a question on the post by Ayten that I hope someone will answer: I am not a nuclear engineer, so I readily acknowledge that I am far from an expert on the subject. But the website Ayten provided gives the branch ratio for decay of U-238 to Th-234 as 0.00005% (along with the half-life given in Dale's response). Would that not also make the amount of beta radiation small?
It was interesting to read in Alex's post about how the presence of U-235 causes alarms to go off. Commercially available (United States, Electron Microscopy Sciences, technical data sheet for Uranyl Acetate, available at www.emsdiasum.com) is listed as 0.1% U235, so there is *some* U235 present, at least for this supplier (Ted Pella lists the composition as 0.3-0.4% U235). EMS also gives a reading for Alpha and Beta radiation for a 100 g sample, and gives a value of .51 uCi/g for the specific activity (they state a material with a value of } 0.002 uCi/g is considered radioactive).
I would also like to agree with Dale on his remarks on the tone of Ayten's response. This should be a place that, even though we may disagree, our mutual respect for one another allows us to be civil and polite, and fosters healthy debates in cases of contention.
Thank you, Jessica
____________________ Jessica Cervantes, MS
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Friday, January 09, 2009 6:42 AM To: Cervantes, Jessica
Dear Ayten,
Your comments are informative but, to my personal taste, definitely a bit negative in tone for this list. Your "schooling" of Alex isn't really necessary and I think we could get your information in a more positive and collegial way.
Regarding the decay tree, I note from the provided link information that
the half-life of U-238 is { {4.468E+9 years} } . It has been a while since
my high school chemistry but I'm wondering how much Th-234 and associated beta emission danger we are really dealing with here; seems like there must be a very small amount of Th-234 produced with such a long half-life of the original U-238. Maybe you could comment on the danger of this.
Thanks,
Dale
celikaktas-at-gmail.com wrote: } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Dear Alex, } } Did you have a chance to check the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } Let me construct the decay tree. I'm sure everybody in this list can } do it but, you keep insisting that "uranyl compounds are alpha } emitters only" so, I will take the time to do the job and post in to } the list. } } Let's start with U-238 which is the starting element in your compound. } } 1) U-238 decays into Th-234 by Alpha decay } } 2) Th-234 decays into Pa-234 by Beta decay } } 3) Pa-234 decays into U-234 by Beta decay } } 4) U-234 decays into Th-230 by Alpha decay } } 5) Th-230 decays into Ra-226 by Alpha decay } } 6) Ra-226 decays into Rn-222 by Alpha decay } } 7) Rn-222 decays into Po-218 by Alpha decay } } 8) Po-218 decays into Pb-214 by Alpha decay } } 9) Pb-214 decays into Bi-214 by Beta decay } } 10) Bi-214 decays into Po-214 by Beta decay } } 11) Po-214 decays into Pb-210 by Alpha decay } } 12) Pb-210 decays into Bi-210 by Beta decay } } 13) Bi-210 decays into Po-210 by Beta decay } } 14) Po-210 decays into Pb-206 by Alpha decay } } Pb-206 is STABLE so, it is the last element to be produced as a result } of U-238 radioactive decay. } } I have constructed the above decay tree using the information from } http://atom.kaeri.re.kr/ton/nuc7.html } While constructing the above decay tree I have used the branch which } has the highest branch ratio (above 99% in each case). } } I do not understand why you are trying to keep things "under control"? } } By the way, I'm a Nuclear Engineer. } } One does not even need to be nuclear engineer to understand this. Even } in high school science classes people learn about radioactive decay } series e.g. A decays into B and B decays into C... } } Best, } Ayten. }
==============================Original Headers============================== 7, 23 -- From dac-at-research.umass.edu Fri Jan 9 08:36:22 2009 7, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09EaMPb032435 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 08:36:22 -0600 7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 23 -- (authenticated bits=0) 7, 23 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n09EaLpI010451 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 09:36:22 -0500 7, 23 -- Message-ID: {49676117.7030802-at-research.umass.edu} 7, 23 -- Date: Fri, 09 Jan 2009 09:37:11 -0500 7, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 23 -- Reply-To: dac-at-research.umass.edu 7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 7, 23 -- MIME-Version: 1.0 7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 23 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters 7, 23 -- only 7, 23 -- References: {200901090743.n097ht8p015453-at-ns.microscopy.com} 7, 23 -- In-Reply-To: {200901090743.n097ht8p015453-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 24, 22 -- From cervantes-at-bendres.com Fri Jan 9 12:37:17 2009 24, 22 -- Received: from smtp.bendres.com (smtp.bendres.com [67.59.84.113]) 24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09IbGAs002132 24, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 12:37:17 -0600 24, 22 -- Content-class: urn:content-classes:message 24, 22 -- MIME-Version: 1.0 24, 22 -- Content-Type: text/plain; 24, 22 -- charset="us-ascii" 24, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 22 -- Subject: RE: [Microscopy] viaWWW: uranyl compounds are alpha emitters 24, 22 -- Date: Fri, 9 Jan 2009 10:37:16 -0800 24, 22 -- Message-ID: {82C755170EE5C44BA26E35A7F6B3864A040C1F-at-dixie.bri.local} 24, 22 -- In-Reply-To: {200901091442.n09Eg4GH008217-at-ns.microscopy.com} 24, 22 -- X-MS-Has-Attach: 24, 22 -- X-MS-TNEF-Correlator: 24, 22 -- Thread-Topic: [Microscopy] viaWWW: uranyl compounds are alpha emitters 24, 22 -- Thread-Index: AclyaHEzMn7sIVgATIeELTj+MUIUqwAFW70Q 24, 22 -- References: {200901091442.n09Eg4GH008217-at-ns.microscopy.com} 24, 22 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 24, 22 -- To: {Microscopy-at-microscopy.com} 24, 22 -- Content-Transfer-Encoding: 8bit 24, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n09IbGAs002132 ==============================End of - Headers==============================
On Jan 9, 2009, at 10:37 AM, cervantes-at-bendres.com wrote:
} I did have a question on the post by Ayten that I hope someone will } answer: I am not a nuclear engineer, so I readily acknowledge that I } am } far from an expert on the subject. But the website Ayten provided } gives } the branch ratio for decay of U-238 to Th-234 as 0.00005% (along with } the half-life given in Dale's response). Would that not also make the } amount of beta radiation small?
Dear Jessica, The amount of Th234 is, indeed, small, but the branching ratio for U238 -} Th234 is close to 100%--the Handbook of Chemistry and Physics also lists SF (spontaneous fission), but this is very rare. Assuming that the process of depleting the U will also get rid of any Th originally mixed in with the natural U, the long half life of U238 means that the amount of Th234 will be small for the first few billion years or so. In any case, the ranges of both alpha and beta particles in glass are smaller than the thickness of the bottle, so a jar of UA will not have either alpha or beta particles traveling to the outside world. The Handbook does also list 3 particle energies for U238 alpha particles, which arise due to the possibility of decay into 3 states of Th234--the ground state and two excited states. These excited states will decay to the ground state, usually by emitting a gamma ray, which will penetrate the jar, a good distance through the air, and your hand (if you are holding the jar). It is these gammas that pose the greatest risk from a sealed jar of UA. For the nit-pickers, the gammas can scatter off atoms in the jar to produce secondary electrons, some of which may be produced close enough to the outer surface of the jar to penetrate into the air, so it is not entirely accurate to say no beta radiation could ever be detected outside the jar. As previously stated by several listers, the major danger from UA is ingestion or inhalation. The same properties of alpha radiation that prevent it from penetrating the dead layer of the skin also dictate that the entire energy of the alpha decay will be deposited in a small volume either on the inner surface of the lung or in the digestive tract and other parts of the body that the U can be transported to or deposited in. This large amount of energy will produce many ion pairs, since one ion pair is produced for every ~30 eV of energy deposited, and the decay energy is 4.268 MeV. The damage done to a cell in which these ions are produced is usually fatal to the cell, but can also be severe without killing the cell, in which case, the cell can become cancerous. Therefore, such activities as weighing UA to make solutions, pipetting UA, which can produce very small droplets that evaporate leaving a small particle of UA, and other handling of UA, especially the solid from the jar, should always be performed with the knowledge that there is risk. ALARA dictates that these procedures be performed in a hood (into which there is a substantial flow of air), and that they are only performed in a limited area, which should be frequently monitored for spilled UA (and any other radioactive materials that might be used) by measuring with a detector and by taking swabs in and around the area that will also be tested for the presence of radiation. Different states and countries have different laws that determine the minimum amount of monitoring, and different institutions have more restrictive policies, so there is no single procedure that is universally followed, but it is very important that each person follow the procedures EHS sets up in one's lab, and even more important that everyone takes safety seriously. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Jan 9 13:38:14 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09JcDnK017471 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 13:38:13 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 592CC32A786 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:38:13 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id E2E4A32A703 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:38:11 -0800 (PST) 6, 22 -- Message-Id: {B018E32F-06FA-4F4B-BE9C-6D4B5EB5C94E-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200901091837.n09IbP7p002260-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] RE: viaWWW: uranyl compounds are alpha emitters 6, 22 -- Date: Fri, 9 Jan 2009 11:38:11 -0800 6, 22 -- References: {200901091837.n09IbP7p002260-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I used to work as a 'radiochemist' making radioactive isotope products and standards, including Uranium. FYI, you can calculate the specific activity, A, from the data you cited:
A = yN, (y should be a 'lambda', sorry!), and A is disintigrations per time (usually sec or min)
y = ln2 / T, where T is half life of isotope. (Convert half-life to seconds to give disintegrations per second.)
N = number of atoms of that isotope (per gram for specific activity).
There are daughter products from the decay, and as you mentioned, there might be U-235 as well, though the manufacturer stated it was clean of this.
By the way, after working as a radio / nuclear chemist, I don't subscribe totally to the ALARA principle. We take about 360mrem/yr background just living in USA. Also, a long roundtrip plane flight will give you up to 10mrem. The risks are negligible.
Hope that helps.
Don
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: cervantes-at-bendres.com [mailto:cervantes-at-bendres.com] Sent: Friday, January 09, 2009 10:47 AM To: dkloos-at-parallaxray.com
I work in a place that does not permit the use of Uranyl salts for EM use because of the radiation dangers (and a healthy respect for ALARA), and am always trying to educate people about what these dangers are with facts. Thus I was very interested in this thread.
I did have a question on the post by Ayten that I hope someone will answer: I am not a nuclear engineer, so I readily acknowledge that I am far from an expert on the subject. But the website Ayten provided gives the branch ratio for decay of U-238 to Th-234 as 0.00005% (along with the half-life given in Dale's response). Would that not also make the amount of beta radiation small?
It was interesting to read in Alex's post about how the presence of U-235 causes alarms to go off. Commercially available (United States, Electron Microscopy Sciences, technical data sheet for Uranyl Acetate, available at www.emsdiasum.com) is listed as 0.1% U235, so there is *some* U235 present, at least for this supplier (Ted Pella lists the composition as 0.3-0.4% U235). EMS also gives a reading for Alpha and Beta radiation for a 100 g sample, and gives a value of .51 uCi/g for the specific activity (they state a material with a value of } 0.002 uCi/g is considered radioactive).
I would also like to agree with Dale on his remarks on the tone of Ayten's response. This should be a place that, even though we may disagree, our mutual respect for one another allows us to be civil and polite, and fosters healthy debates in cases of contention.
Thank you, Jessica
____________________ Jessica Cervantes, MS
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Friday, January 09, 2009 6:42 AM To: Cervantes, Jessica
Dear Ayten,
Your comments are informative but, to my personal taste, definitely a bit negative in tone for this list. Your "schooling" of Alex isn't really necessary and I think we could get your information in a more positive and collegial way.
Regarding the decay tree, I note from the provided link information that
the half-life of U-238 is { {4.468E+9 years} } . It has been a while since
my high school chemistry but I'm wondering how much Th-234 and associated beta emission danger we are really dealing with here; seems like there must be a very small amount of Th-234 produced with such a long half-life of the original U-238. Maybe you could comment on the danger of this.
Thanks,
Dale
celikaktas-at-gmail.com wrote: } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Dear Alex, } } Did you have a chance to check the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } Let me construct the decay tree. I'm sure everybody in this list can } do it but, you keep insisting that "uranyl compounds are alpha } emitters only" so, I will take the time to do the job and post in to } the list. } } Let's start with U-238 which is the starting element in your compound. } } 1) U-238 decays into Th-234 by Alpha decay } } 2) Th-234 decays into Pa-234 by Beta decay } } 3) Pa-234 decays into U-234 by Beta decay } } 4) U-234 decays into Th-230 by Alpha decay } } 5) Th-230 decays into Ra-226 by Alpha decay } } 6) Ra-226 decays into Rn-222 by Alpha decay } } 7) Rn-222 decays into Po-218 by Alpha decay } } 8) Po-218 decays into Pb-214 by Alpha decay } } 9) Pb-214 decays into Bi-214 by Beta decay } } 10) Bi-214 decays into Po-214 by Beta decay } } 11) Po-214 decays into Pb-210 by Alpha decay } } 12) Pb-210 decays into Bi-210 by Beta decay } } 13) Bi-210 decays into Po-210 by Beta decay } } 14) Po-210 decays into Pb-206 by Alpha decay } } Pb-206 is STABLE so, it is the last element to be produced as a result } of U-238 radioactive decay. } } I have constructed the above decay tree using the information from } http://atom.kaeri.re.kr/ton/nuc7.html } While constructing the above decay tree I have used the branch which } has the highest branch ratio (above 99% in each case). } } I do not understand why you are trying to keep things "under control"? } } By the way, I'm a Nuclear Engineer. } } One does not even need to be nuclear engineer to understand this. Even } in high school science classes people learn about radioactive decay } series e.g. A decays into B and B decays into C... } } Best, } Ayten. }
==============================Original Headers============================== 7, 23 -- From dac-at-research.umass.edu Fri Jan 9 08:36:22 2009 7, 23 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09EaMPb032435 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 08:36:22 -0600 7, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 23 -- (authenticated bits=0) 7, 23 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n09EaLpI010451 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 09:36:22 -0500 7, 23 -- Message-ID: {49676117.7030802-at-research.umass.edu} 7, 23 -- Date: Fri, 09 Jan 2009 09:37:11 -0500 7, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 23 -- Reply-To: dac-at-research.umass.edu 7, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 7, 23 -- MIME-Version: 1.0 7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 23 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters 7, 23 -- only 7, 23 -- References: {200901090743.n097ht8p015453-at-ns.microscopy.com} 7, 23 -- In-Reply-To: {200901090743.n097ht8p015453-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 24, 22 -- From cervantes-at-bendres.com Fri Jan 9 12:37:17 2009 24, 22 -- Received: from smtp.bendres.com (smtp.bendres.com [67.59.84.113]) 24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09IbGAs002132 24, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 12:37:17 -0600 24, 22 -- Content-class: urn:content-classes:message 24, 22 -- MIME-Version: 1.0 24, 22 -- Content-Type: text/plain; 24, 22 -- charset="us-ascii" 24, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 22 -- Subject: RE: [Microscopy] viaWWW: uranyl compounds are alpha emitters 24, 22 -- Date: Fri, 9 Jan 2009 10:37:16 -0800 24, 22 -- Message-ID: {82C755170EE5C44BA26E35A7F6B3864A040C1F-at-dixie.bri.local} 24, 22 -- In-Reply-To: {200901091442.n09Eg4GH008217-at-ns.microscopy.com} 24, 22 -- X-MS-Has-Attach: 24, 22 -- X-MS-TNEF-Correlator: 24, 22 -- Thread-Topic: [Microscopy] viaWWW: uranyl compounds are alpha emitters 24, 22 -- Thread-Index: AclyaHEzMn7sIVgATIeELTj+MUIUqwAFW70Q 24, 22 -- References: {200901091442.n09Eg4GH008217-at-ns.microscopy.com} 24, 22 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 24, 22 -- To: {Microscopy-at-microscopy.com} 24, 22 -- Content-Transfer-Encoding: 8bit 24, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n09IbGAs002132 ==============================End of - Headers==============================
==============================Original Headers============================== 41, 30 -- From dkloos-at-parallaxray.com Fri Jan 9 13:44:23 2009 41, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) 41, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09JiLtE026720 41, 30 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 13:44:22 -0600 41, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) 41, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) 41, 30 -- (envelope-from {dkloos-at-parallaxray.com} ) 41, 30 -- id 1LLNHN-000AxF-NI; Fri, 09 Jan 2009 12:44:25 -0700 41, 30 -- Reply-To: {dkloos-at-parallaxray.com} 41, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com} 41, 30 -- To: {cervantes-at-bendres.com} 41, 30 -- Cc: {microscopy-at-microscopy.com} 41, 30 -- References: {200901091847.n09IlB3t015536-at-ns.microscopy.com} 41, 30 -- Subject: RE: [Microscopy] RE: viaWWW: uranyl compounds are alpha emitters 41, 30 -- Date: Fri, 9 Jan 2009 11:44:13 -0800 41, 30 -- Organization: Parallax Research 41, 30 -- Message-ID: {E956386CB52E4F8488CB5A61C09C99D8-at-donl} 41, 30 -- MIME-Version: 1.0 41, 30 -- Content-Type: text/plain; 41, 30 -- charset="us-ascii" 41, 30 -- Content-Transfer-Encoding: 7bit 41, 30 -- X-Mailer: Microsoft Office Outlook 11 41, 30 -- Thread-Index: AclyirXsDrkzft58SYy5CgCv2E93aQABANEw 41, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 41, 30 -- In-Reply-To: {200901091847.n09IlB3t015536-at-ns.microscopy.com} 41, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 41, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com 41, 30 -- X-AntiAbuse: Original Domain - microscopy.com 41, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] 41, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com ==============================End of - Headers==============================
On Jan 9, 2009, at 11:44 AM, dkloos-at-parallaxray.com wrote:
} By the way, after working as a radio / nuclear chemist, I don't } subscribe } totally to the ALARA principle. We take about 360mrem/yr background } just } living in USA. Also, a long roundtrip plane flight will give you up } to } 10mrem. The risks are negligible.
Dear Don, It is very true that there is background radiation, and it is still not known whether any increases over background cause a proportional increase in risk, but since the R stands for "reasonably", ALARA should be followed. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Jan 9 13:56:58 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09JuwMj012369 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 13:56:58 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id DBC3C328E87 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:56:57 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id DA21532A846 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 11:56:56 -0800 (PST) 6, 22 -- Message-Id: {D4A7F8CA-339B-441B-ADF1-3F617A2A4FD4-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200901091944.n09JiUPN026950-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters 6, 22 -- Date: Fri, 9 Jan 2009 11:56:56 -0800 6, 22 -- References: {200901091944.n09JiUPN026950-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Thanks to all of you who have responded to my recent query. I'm sure that I will have more as time passes, and I appreciate the fantastic response that I've had from the list.
Since we are on the topic of the dangers of EM work (the uranyl acetate thread), I have a question about cacodylate buffer. I'm getting set to teach an introductory EM course for biologists to undergraduates. Having interviewed two of my three students during the previous semester, I know that I will be starting very much at zero. They had no to little knowledge of what "EM" is or can be used for before I spoke with them - they are exploring this new class. My plan is to take them through the preparation of plant, animal, and some sort of micro sample via traditional chemical fixation methods and keep it as simple as possible. I am inclined to steer clear of cacodylate buffer due to its toxicity and because they have enough to deal with already, and stick with phosphate buffer. However, I have noticed that most if not all of the animal tissue protocols I've been perusing use cac buffer. Is there any reason why I should keep it in the protocol?
Thanks for your advice. Kristen
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology 202 Compton Science Center Frostburg State University 101 Braddock Road Frostburg, MD 21532
k.lennon-at-frostburg.edu
==============================Original Headers============================== 7, 20 -- From kamlennon-at-yahoo.com Fri Jan 9 13:57:15 2009 7, 20 -- Received: from web84004.mail.mud.yahoo.com (web84004.mail.mud.yahoo.com [68.142.206.174]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09JvEx0012745 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 13:57:14 -0600 7, 20 -- Received: (qmail 76571 invoked by uid 60001); 9 Jan 2009 19:57:14 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 20 -- b=xeLr+fOkc5vJ3EXto1Y6+o7SUjRsZmyWr021p/AR8+eZSkJdh5uAKRIqv7XOXlDD/DxqNcT7zKQfKkLZ8xb+qim3FguZ95OneP3PcPPuzZm7XdHTVOeaRk/Onkfmz9gH67KG3edVJa6kzqjAXn/Wmi6J6hcnTSKOM2Iqc98V+Tg=; 7, 20 -- X-YMail-OSG: LFU8udYVM1kiLkk70s8A_YJn70xTwWxw7CGSMeW3AL1T.rnd4LZsOXO_lJUYOJekMg-- 7, 20 -- Received: from [96.239.149.81] by web84004.mail.mud.yahoo.com via HTTP; Fri, 09 Jan 2009 11:57:13 PST 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 7, 20 -- Date: Fri, 9 Jan 2009 11:57:13 -0800 (PST) 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} 7, 20 -- Reply-To: kamlennon-at-yahoo.com 7, 20 -- Subject: Cac buffer and undergrads - chancy? 7, 20 -- To: Microscopy-at-Microscopy.Com 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Message-ID: {982974.76126.qm-at-web84004.mail.mud.yahoo.com} ==============================End of - Headers==============================
There are 2 ways of looking at this: first, phosphate and other buffers work as well as cacodylate, the difference between cac and PO4, e.g., is mostly preference and what one is used to seeing. So why use a toxin that can be avoided? Especially since some people (like me) are sensitive to the arsenic. The other way is: sometimes cacodylate is needed, and the students will have to be using other toxic materials in microscopy and chemistry and biotechnology and ... in other words, they need to learn how to properly handle such materials, and the sooner they learn the better, so use it.
There, microambiguity. In our EM courses, I avoid cacodylate (because I'm sensitive), but we do make it available if needed, or if a student wants to use it for a project.
Generally, though, when you're teaching about buffers, there are a lot more to discuss than just PO4 or cacodylate. If the class is doing aquatic critters (algae, protistans, tiny inverts, and suchlike), then the best buffer is 0.02 micron filtered water from where the crittere were collected.
Phil
} Hello Again Listers, } } Thanks to all of you who have responded to my recent query. I'm sure } that I will have more as time passes, and I appreciate the fantastic } response that I've had from the list. } } Since we are on the topic of the dangers of EM work (the uranyl } acetate thread), I have a question about cacodylate buffer. I'm } getting set to teach an introductory EM course for biologists to } undergraduates. Having interviewed two of my three students during } the previous semester, I know that I will be starting very much at } zero. They had no to little knowledge of what "EM" is or can be used } for before I spoke with them - they are exploring this new class. My } plan is to take them through the preparation of plant, animal, and } some sort of micro sample via traditional chemical fixation methods } and keep it as simple as possible. I am inclined to steer clear of } cacodylate buffer due to its toxicity and because they have enough } to deal with already, and stick with phosphate buffer. However, I } have noticed that most if not all of the animal tissue protocols } I've been perusing use cac buffer. Is there any reason why I should } keep it in the protocol? } } Thanks for your advice. } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } } k.lennon-at-frostburg.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 25 -- From oshel1pe-at-cmich.edu Fri Jan 9 14:13:13 2009 6, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KDCn7007223 6, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:13:13 -0600 6, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n09KDBgb017674 6, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:13:11 -0500 6, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 6, 25 -- Fri, 9 Jan 2009 15:13:02 -0500 6, 25 -- Mime-Version: 1.0 6, 25 -- Message-Id: {f06240804c58d5e6fc0f6-at-[141.209.160.249]} 6, 25 -- In-Reply-To: {200901092000.n09K0PVw024065-at-ns.microscopy.com} 6, 25 -- References: {200901092000.n09K0PVw024065-at-ns.microscopy.com} 6, 25 -- Date: Fri, 9 Jan 2009 15:13:00 -0500 6, 25 -- To: Microscopy-at-microscopy.com 6, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 25 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy? 6, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 25 -- X-OriginalArrivalTime: 09 Jan 2009 20:13:02.0896 (UTC) FILETIME=[ACF1D300:01C97296] 6, 25 -- X-Canit-CHI2: 0.00 6, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 25 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 6, 25 -- X-CanItPRO-Stream: default 6, 25 -- X-Canit-Stats-ID: 7251385 - 9ad74a6e6749 6, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
As a nuclear engineer you should know better about dangers of radioactivity (by the way, by training I am a physicist.)
Decay tree says us nothing about these dangers. We should know real levels of radiation. What can you say about them? Is level of radiation at 5 inches from open bottle with 25 g of uranil acetate much greater then the one from granite countertop in someone kitchen? I do not know. Since I do not know, I will be cautious, but just cautious, not paranoid.
Let’s see what World Health Association says: http://www.who.int/mediacentre/factsheets/fs257/en/
“DEPLETED URANIUM • On average, approximately 90 µg (micrograms) of uranium exists in the human body from normal intakes of water, food and air. About 66% is found in the skeleton, 16% in the liver, 8% in the kidneys and 10% in other tissues. {90 micrograms of uranium, not depleted uranium} • The uranium remaining after removal of the enriched fraction contains about 99.8% 238U, 0.2% 235U and 0.001% 234U by mass; this is referred to as depleted uranium or DU. • Under most circumstances, use of DU will make a negligible contribution to the overall natural background levels of uranium in the environment. Probably the greatest potential for DU exposure will follow conflict where DU munitions are used. • Average annual intakes of uranium by adults are estimated to be about 0.5mg (500 μg) from ingestion of food and water and 0.6 μg from breathing air.â€
So, uranium is everywhere. We should not bother ourselves with decay tree. If in doubt, we should measure radiation. And, of course, we should remember about toxicity of uranium. Gloves, fume hoods, proper treatment of spillage, etc. And when required, we should collect and dispose used chemicals in proper way.
I beg your pardon if you find my reply a bit harsh. I just followed your style.
Regards,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] } Sent: Friday, January 09, 2009 1:39 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha } emitters only } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Dear Alex, } } Did you have a chance to check the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } Let me construct the decay tree. I'm sure everybody in this } list can do it but, you keep insisting that "uranyl compounds } are alpha emitters only" so, I will take the time to do the } job and post in to the list. } } Let's start with U-238 which is the starting element in your compound. } } 1) U-238 decays into Th-234 by Alpha decay } } 2) Th-234 decays into Pa-234 by Beta decay } } 3) Pa-234 decays into U-234 by Beta decay } } 4) U-234 decays into Th-230 by Alpha decay } } 5) Th-230 decays into Ra-226 by Alpha decay } } 6) Ra-226 decays into Rn-222 by Alpha decay } } 7) Rn-222 decays into Po-218 by Alpha decay } } 8) Po-218 decays into Pb-214 by Alpha decay } } 9) Pb-214 decays into Bi-214 by Beta decay } } 10) Bi-214 decays into Po-214 by Beta decay } } 11) Po-214 decays into Pb-210 by Alpha decay } } 12) Pb-210 decays into Bi-210 by Beta decay } } 13) Bi-210 decays into Po-210 by Beta decay } } 14) Po-210 decays into Pb-206 by Alpha decay } } Pb-206 is STABLE so, it is the last element to be produced as } a result of U-238 radioactive decay. } } I have constructed the above decay tree using the information } from http://atom.kaeri.re.kr/ton/nuc7.html } While constructing the above decay tree I have used the } branch which has the highest branch ratio (above 99% in each case). } } I do not understand why you are trying to keep things "under control"? } } By the way, I'm a Nuclear Engineer. } } One does not even need to be nuclear engineer to understand } this. Even in high school science classes people learn about } radioactive decay series e.g. A decays into B and B decays into C... } } Best, } Ayten. } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } =========================== } } } On Fri, Jan 9, 2009 at 2:27 AM, {abesenyo-at-ibilabs.com} wrote: } } } } Email: abesenyo-at-ibilabs.com } } Name: Alex Besenyo PhD } } } } Organization: ibilabs } } } } Title-Subject: [Filtered] uranyl compounds are alpha emitters only } } } } Question: Question: } } } } Is it true that the stuff we use has been somehow } } depleted, so that it isn't as radioactive as "real" uranyl } } salts? Or is this yet another old wive's tale of EM?! } } } } Reply: } } } } When we manufacture these compounds we purchase the raw uranium in a } } depleted state from the government. There is no chance for error } } here. We do not use natural uranium. } } } } This means that the enrichable uranium U-235 has been removed. } } The then U-238 which only emitts alpha radiation is procesed. } } } } The term "depleted" means that U-235 has been removed. } } } } If even by the slightest chance that U235 were present then every } } alarm would go off in our facility because Beta and Gamma radiation } } is detected. } } } } I hope this answers everybodies concerns. } } } } Our products are sold exclusively through a distributor network and } } all of them have been instructed on this information. } } } } I only responded when I saw the original post and I had to respond } } before it got out of control. } } } } Sincerely } } Alex Besenyo PhD } } } } } } } } ==============================Original } Headers============================== } 28, 37 -- From celikaktas-at-gmail.com Fri Jan 9 01:38:48 2009 } 28, 37 -- Received: from mail-bw0-f12.google.com } (mail-bw0-f12.google.com [209.85.218.12]) } 28, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n097cllZ007406 } 28, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan } 2009 01:38:48 -0600 } 28, 37 -- Received: by bwz5 with SMTP id 5so20323150bwz.18 } 28, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 08 } Jan 2009 23:38:46 -0800 (PST) } 28, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 28, 37 -- d=gmail.com; s=gamma; } 28, 37 -- } h=domainkey-signature:received:received:message-id:date:from:to } 28, 37 -- :subject:in-reply-to:mime-version:content-type } 28, 37 -- } :content-transfer-encoding:content-disposition:references; } 28, 37 -- bh=x3VBln30706ul3mo8rzkKk8tdVV0mnTtpGId5uaYeEM=; } 28, 37 -- } b=qBVYz0biX1/wFlDLibyks38NncsgFjqElzGSuPXOiX+ZCB902M5i3lsMmCryurouqZ } 28, 37 -- } q2vvqo4qq+LQCiFwGfBQltrqfo1jiWYiWnPMwvN3V4b9MDoZd/yjgF76rgbzKDtibrjg } 28, 37 -- Ob/1zXIpi/2mHaPBWSu4mzAG29Fqx67ZL1YFg= } 28, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 28, 37 -- d=gmail.com; s=gamma; } 28, 37 -- } h=message-id:date:from:to:subject:in-reply-to:mime-version } 28, 37 -- } :content-type:content-transfer-encoding:content-disposition } 28, 37 -- :references; } 28, 37 -- } b=DNSaPkpBjjjni4T+G+pPUuxo3M1nntLysfMKUnfOWQUEElcsWGJ3PmM4uVFQBbtTpT } 28, 37 -- } g+IO1lpZUOd3KJn43j3ed/pgCrIp1p0oTRjteC1sfQN2njKF2xkzfFZtTt2rNMbo4jBE } 28, 37 -- /h849iT1rgdv5cvNLmKbW91LhMbKuR486Rzbg= } 28, 37 -- Received: by 10.103.217.7 with SMTP id } u7mr1380019muq.125.1231486725631; } 28, 37 -- Thu, 08 Jan 2009 23:38:45 -0800 (PST) } 28, 37 -- Received: by 10.102.247.16 with HTTP; Thu, 8 Jan } 2009 23:38:45 -0800 (PST) } 28, 37 -- Message-ID: } {1075c5c10901082338y14d2b189m26ca5f198f5a9454-at-mail.gmail.com} } 28, 37 -- Date: Fri, 9 Jan 2009 09:38:45 +0200 } 28, 37 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} } 28, 37 -- To: abesenyo-at-ibilabs.com, microscopy } {Microscopy-at-microscopy.com} } 28, 37 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds } are alpha emitters only } 28, 37 -- In-Reply-To: {200901090027.n090RYH1022494-at-ns.microscopy.com} } 28, 37 -- MIME-Version: 1.0 } 28, 37 -- Content-Type: text/plain; charset=UTF-8 } 28, 37 -- Content-Transfer-Encoding: 7bit } 28, 37 -- Content-Disposition: inline } 28, 37 -- References: {200901090027.n090RYH1022494-at-ns.microscopy.com} } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 14, 25 -- From DusevichV-at-umkc.edu Fri Jan 9 14:13:53 2009 14, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KDquJ008557 14, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:13:52 -0600 14, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 25 -- Fri, 9 Jan 2009 14:13:44 -0600 14, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 25 -- Content-class: urn:content-classes:message 14, 25 -- MIME-Version: 1.0 14, 25 -- Content-Type: text/plain; 14, 25 -- charset="UTF-8" 14, 25 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only 14, 25 -- Date: Fri, 9 Jan 2009 14:13:44 -0600 14, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AA-at-KC-MSX1.kc.umkc.edu} 14, 25 -- In-Reply-To: {200901090739.n097dQZk008263-at-ns.microscopy.com} 14, 25 -- X-MS-Has-Attach: 14, 25 -- X-MS-TNEF-Correlator: 14, 25 -- Thread-Topic: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only 14, 25 -- thread-index: AclyLWbfA0iBUCGrQXeRBZzilk1+5QAaS2kQ 14, 25 -- References: {200901090739.n097dQZk008263-at-ns.microscopy.com} 14, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 14, 25 -- To: {Microscopy-at-microscopy.com} 14, 25 -- X-OriginalArrivalTime: 09 Jan 2009 20:13:44.0274 (UTC) FILETIME=[C59B9B20:01C97296] 14, 25 -- Content-Transfer-Encoding: 8bit 14, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n09KDquJ008557 ==============================End of - Headers==============================
I have found this conversation both enlightening and interesting. One of the things I found interesting is to guess at the location of the submitter. This gives me a measure of my bias. I will say that we have flogged this horse to death, but I want to share a bit of my bias.
The total elimination of risk, no matter if you perceive it as reasonable or not, seems to be a major pre-occupation of our society. This seems to be driven by people whose livelihood is derived from telling us about these dangers. One might expect a bias from them. After all if they are not making you safer, they are not doing their job.
It is difficult to argue against safety precautions and still seem rational, it appears to me that we are taking safety to unreasonable heights. We need to be reminded that nobody gets out of this world alive. You can stay hidden under your bed covers your entire life and bad things will still happen to good people.
At one company I am familiar with, the chemists are not allowed to use toluene. It seems the company believes it just too dangerous to be used by trained professionals.
I suggest you weight the potential dangers from UA against all the dangers from that glass of red wine. I suspect the alcohol is more dangerous.
Having said my 2cents, I’m heading home for grilled meat and a beer.
Living on the edge…… Frank Karl
DusevichV-at-umkc.ed u To 01/09/2009 03:21 frank_karl-at-lincolnelectric.com PM cc
Subject Please respond to [Microscopy] viaWWW: uranyl DusevichV-at-umkc.ed compounds are alpha emitters only u
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Ayten,
As a nuclear engineer you should know better about dangers of radioactivity (by the way, by training I am a physicist.)
Decay tree says us nothing about these dangers. We should know real levels of radiation. What can you say about them? Is level of radiation at 5 inches from open bottle with 25 g of uranil acetate much greater then the one from granite countertop in someone kitchen? I do not know. Since I do not know, I will be cautious, but just cautious, not paranoid.
Let’s see what World Health Association says: http://www.who.int/mediacentre/factsheets/fs257/en/
“DEPLETED URANIUM • On average, approximately 90 µg (micrograms) of uranium exists in the human body from normal intakes of water, food and air. About 66% is found in the skeleton, 16% in the liver, 8% in the kidneys and 10% in other tissues. {90 micrograms of uranium, not depleted uranium} • The uranium remaining after removal of the enriched fraction contains about 99.8% 238U, 0.2% 235U and 0.001% 234U by mass; this is referred to as depleted uranium or DU. • Under most circumstances, use of DU will make a negligible contribution to the overall natural background levels of uranium in the environment. Probably the greatest potential for DU exposure will follow conflict where DU munitions are used. • Average annual intakes of uranium by adults are estimated to be about 0.5mg (500 μg) from ingestion of food and water and 0.6 μg from breathing air.â€Â
So, uranium is everywhere. We should not bother ourselves with decay tree. If in doubt, we should measure radiation. And, of course, we should remember about toxicity of uranium. Gloves, fume hoods, proper treatment of spillage, etc. And when required, we should collect and dispose used chemicals in proper way.
I beg your pardon if you find my reply a bit harsh. I just followed your style.
Regards,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] } Sent: Friday, January 09, 2009 1:39 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha } emitters only } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Dear Alex, } } Did you have a chance to check the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } Let me construct the decay tree. I'm sure everybody in this } list can do it but, you keep insisting that "uranyl compounds } are alpha emitters only" so, I will take the time to do the } job and post in to the list. } } Let's start with U-238 which is the starting element in your compound. } } 1) U-238 decays into Th-234 by Alpha decay } } 2) Th-234 decays into Pa-234 by Beta decay } } 3) Pa-234 decays into U-234 by Beta decay } } 4) U-234 decays into Th-230 by Alpha decay } } 5) Th-230 decays into Ra-226 by Alpha decay } } 6) Ra-226 decays into Rn-222 by Alpha decay } } 7) Rn-222 decays into Po-218 by Alpha decay } } 8) Po-218 decays into Pb-214 by Alpha decay } } 9) Pb-214 decays into Bi-214 by Beta decay } } 10) Bi-214 decays into Po-214 by Beta decay } } 11) Po-214 decays into Pb-210 by Alpha decay } } 12) Pb-210 decays into Bi-210 by Beta decay } } 13) Bi-210 decays into Po-210 by Beta decay } } 14) Po-210 decays into Pb-206 by Alpha decay } } Pb-206 is STABLE so, it is the last element to be produced as } a result of U-238 radioactive decay. } } I have constructed the above decay tree using the information } from http://atom.kaeri.re.kr/ton/nuc7.html. } While constructing the above decay tree I have used the } branch which has the highest branch ratio (above 99% in each case). } } I do not understand why you are trying to keep things "under control"? } } By the way, I'm a Nuclear Engineer. } } One does not even need to be nuclear engineer to understand } this. Even in high school science classes people learn about } radioactive decay series e.g. A decays into B and B decays into C... } } Best, } Ayten. } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } =========================== } } } On Fri, Jan 9, 2009 at 2:27 AM, {abesenyo-at-ibilabs.com} wrote: } } } } Email: abesenyo-at-ibilabs.com } } Name: Alex Besenyo PhD } } } } Organization: ibilabs } } } } Title-Subject: [Filtered] uranyl compounds are alpha emitters only } } } } Question: Question: } } } } Is it true that the stuff we use has been somehow } } depleted, so that it isn't as radioactive as "real" uranyl } } salts? Or is this yet another old wive's tale of EM?! } } } } Reply: } } } } When we manufacture these compounds we purchase the raw uranium in a } } depleted state from the government. There is no chance for error } } here. We do not use natural uranium. } } } } This means that the enrichable uranium U-235 has been removed. } } The then U-238 which only emitts alpha radiation is procesed. } } } } The term "depleted" means that U-235 has been removed. } } } } If even by the slightest chance that U235 were present then every } } alarm would go off in our facility because Beta and Gamma radiation } } is detected. } } } } I hope this answers everybodies concerns. } } } } Our products are sold exclusively through a distributor network and } } all of them have been instructed on this information. } } } } I only responded when I saw the original post and I had to respond } } before it got out of control. } } } } Sincerely } } Alex Besenyo PhD } } } } } } } } ==============================Original } Headers============================== } 28, 37 -- From celikaktas-at-gmail.com Fri Jan 9 01:38:48 2009 } 28, 37 -- Received: from mail-bw0-f12.google.com } (mail-bw0-f12.google.com [209.85.218.12]) } 28, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n097cllZ007406 } 28, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan } 2009 01:38:48 -0600 } 28, 37 -- Received: by bwz5 with SMTP id 5so20323150bwz.18 } 28, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 08 } Jan 2009 23:38:46 -0800 (PST) } 28, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 28, 37 -- d=gmail.com; s=gamma; } 28, 37 -- } h=domainkey-signature:received:received:message-id:date:from:to } 28, 37 -- :subject:in-reply-to:mime-version:content-type } 28, 37 -- } :content-transfer-encoding:content-disposition:references; } 28, 37 -- bh=x3VBln30706ul3mo8rzkKk8tdVV0mnTtpGId5uaYeEM=; } 28, 37 -- } b=qBVYz0biX1/wFlDLibyks38NncsgFjqElzGSuPXOiX+ZCB902M5i3lsMmCryurouqZ } 28, 37 -- } q2vvqo4qq+LQCiFwGfBQltrqfo1jiWYiWnPMwvN3V4b9MDoZd/yjgF76rgbzKDtibrjg } 28, 37 -- Ob/1zXIpi/2mHaPBWSu4mzAG29Fqx67ZL1YFg= } 28, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 28, 37 -- d=gmail.com; s=gamma; } 28, 37 -- } h=message-id:date:from:to:subject:in-reply-to:mime-version } 28, 37 -- } :content-type:content-transfer-encoding:content-disposition } 28, 37 -- :references; } 28, 37 -- } b=DNSaPkpBjjjni4T+G+pPUuxo3M1nntLysfMKUnfOWQUEElcsWGJ3PmM4uVFQBbtTpT } 28, 37 -- } g+IO1lpZUOd3KJn43j3ed/pgCrIp1p0oTRjteC1sfQN2njKF2xkzfFZtTt2rNMbo4jBE } 28, 37 -- /h849iT1rgdv5cvNLmKbW91LhMbKuR486Rzbg= } 28, 37 -- Received: by 10.103.217.7 with SMTP id } u7mr1380019muq.125.1231486725631; } 28, 37 -- Thu, 08 Jan 2009 23:38:45 -0800 (PST) } 28, 37 -- Received: by 10.102.247.16 with HTTP; Thu, 8 Jan } 2009 23:38:45 -0800 (PST) } 28, 37 -- Message-ID: } {1075c5c10901082338y14d2b189m26ca5f198f5a9454-at-mail.gmail.com} } 28, 37 -- Date: Fri, 9 Jan 2009 09:38:45 +0200 } 28, 37 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} } 28, 37 -- To: abesenyo-at-ibilabs.com, microscopy } {Microscopy-at-microscopy.com} } 28, 37 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds } are alpha emitters only } 28, 37 -- In-Reply-To: {200901090027.n090RYH1022494-at-ns.microscopy.com} } 28, 37 -- MIME-Version: 1.0 } 28, 37 -- Content-Type: text/plain; charset=UTF-8 } 28, 37 -- Content-Transfer-Encoding: 7bit } 28, 37 -- Content-Disposition: inline } 28, 37 -- References: {200901090027.n090RYH1022494-at-ns.microscopy.com} } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 14, 25 -- From DusevichV-at-umkc.edu Fri Jan 9 14:13:53 2009 14, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KDquJ008557 14, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:13:52 -0600 14, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 25 -- Fri, 9 Jan 2009 14:13:44 -0600 14, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 25 -- Content-class: urn:content-classes:message 14, 25 -- MIME-Version: 1.0 14, 25 -- Content-Type: text/plain; 14, 25 -- charset="UTF-8" 14, 25 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only 14, 25 -- Date: Fri, 9 Jan 2009 14:13:44 -0600 14, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AA-at-KC-MSX1.kc.umkc.edu} 14, 25 -- In-Reply-To: {200901090739.n097dQZk008263-at-ns.microscopy.com} 14, 25 -- X-MS-Has-Attach: 14, 25 -- X-MS-TNEF-Correlator: 14, 25 -- Thread-Topic: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters only 14, 25 -- thread-index: AclyLWbfA0iBUCGrQXeRBZzilk1+5QAaS2kQ 14, 25 -- References: {200901090739.n097dQZk008263-at-ns.microscopy.com} 14, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 14, 25 -- To: {Microscopy-at-microscopy.com} 14, 25 -- X-OriginalArrivalTime: 09 Jan 2009 20:13:44.0274 (UTC) FILETIME= [C59B9B20:01C97296] 14, 25 -- Content-Transfer-Encoding: 8bit 14, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n09KDquJ008557 ==============================End of - Headers==============================
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==============================Original Headers============================== 38, 24 -- From frank_karl-at-lincolnelectric.com Fri Jan 9 14:31:19 2009 38, 24 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 38, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KVJT7000814 38, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:31:19 -0600 38, 24 -- In-Reply-To: {200901092021.n09KLUr1031349-at-ns.microscopy.com} 38, 24 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters only 38, 24 -- To: DusevichV-at-umkc.edu, Microscopy-at-microscopy.com 38, 24 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 38, 24 -- Message-ID: {OFE5145C76.CBE47B69-ON85257539.00708B12-85257539.0070B254-at-lincolnelectric.com} 38, 24 -- Date: Fri, 9 Jan 2009 15:31:02 -0500 38, 24 -- From: Frank_Karl-at-lincolnelectric.com 38, 24 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 38, 24 -- 07, 2008) at 01/09/2009 03:31:04 PM, 38, 24 -- CD-MIME complete at 01/09/2009 03:31:04 PM, 38, 24 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 38, 24 -- 07, 2008) at 01/09/2009 03:31:04 PM, 38, 24 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 38, 24 -- 07, 2008) at 01/09/2009 03:31:04 PM, 38, 24 -- Serialize complete at 01/09/2009 03:31:04 PM 38, 24 -- MIME-Version: 1.0 38, 24 -- Content-Type: text/plain; 38, 24 -- charset="UTF-8" 38, 24 -- Content-Transfer-Encoding: 8bit 38, 24 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n09KVJT7000814 ==============================End of - Headers==============================
There is no compelling reason to use cacodylate buffers for an introductory class. If you need to add calcium to the fix you can use HEPES or PIPES, plenty of literature on these. Cacodylate was very convenient because it was a one salt buffer and Ca ions did not precipitate. Hazardous waste disposal concerns have made its use difficult to justify.
Geoff
kamlennon-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Again Listers, } } Thanks to all of you who have responded to my recent query. I'm sure that I will have more as time passes, and I appreciate the fantastic response that I've had from the list. } } Since we are on the topic of the dangers of EM work (the uranyl acetate thread), I have a question about cacodylate buffer. I'm getting set to teach an introductory EM course for biologists to undergraduates. Having interviewed two of my three students during the previous semester, I know that I will be starting very much at zero. They had no to little knowledge of what "EM" is or can be used for before I spoke with them - they are exploring this new class. My plan is to take them through the preparation of plant, animal, and some sort of micro sample via traditional chemical fixation methods and keep it as simple as possible. I am inclined to steer clear of cacodylate buffer due to its toxicity and because they have enough to deal with already, and stick with phosphate buffer. However, I have noticed that most if not all of the animal tissue protocols I've been perusing use cac buffer. Is there any reason why I should keep it in the protocol? } } Thanks for your advice. } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } } k.lennon-at-frostburg.edu } } } } } ==============================Original Headers============================== } 7, 20 -- From kamlennon-at-yahoo.com Fri Jan 9 13:57:15 2009 } 7, 20 -- Received: from web84004.mail.mud.yahoo.com (web84004.mail.mud.yahoo.com [68.142.206.174]) } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09JvEx0012745 } 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 13:57:14 -0600 } 7, 20 -- Received: (qmail 76571 invoked by uid 60001); 9 Jan 2009 19:57:14 -0000 } 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 7, 20 -- s=s1024; d=yahoo.com; } 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; } 7, 20 -- b=xeLr+fOkc5vJ3EXto1Y6+o7SUjRsZmyWr021p/AR8+eZSkJdh5uAKRIqv7XOXlDD/DxqNcT7zKQfKkLZ8xb+qim3FguZ95OneP3PcPPuzZm7XdHTVOeaRk/Onkfmz9gH67KG3edVJa6kzqjAXn/Wmi6J6hcnTSKOM2Iqc98V+Tg=; } 7, 20 -- X-YMail-OSG: LFU8udYVM1kiLkk70s8A_YJn70xTwWxw7CGSMeW3AL1T.rnd4LZsOXO_lJUYOJekMg-- } 7, 20 -- Received: from [96.239.149.81] by web84004.mail.mud.yahoo.com via HTTP; Fri, 09 Jan 2009 11:57:13 PST } 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 } 7, 20 -- Date: Fri, 9 Jan 2009 11:57:13 -0800 (PST) } 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } 7, 20 -- Reply-To: kamlennon-at-yahoo.com } 7, 20 -- Subject: Cac buffer and undergrads - chancy? } 7, 20 -- To: Microscopy-at-Microscopy.Com } 7, 20 -- MIME-Version: 1.0 } 7, 20 -- Content-Type: text/plain; charset=us-ascii } 7, 20 -- Message-ID: {982974.76126.qm-at-web84004.mail.mud.yahoo.com} } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 28 -- From mcauliff-at-umdnj.edu Fri Jan 9 14:32:36 2009 9, 28 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09KWaoa003266 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:32:36 -0600 9, 28 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 9, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 80F2F4C05F 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:32:35 -0500 (EST) 9, 28 -- Received: from umdnj.edu (unknown [10.32.15.102]) 9, 28 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 1757550C155 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:20:18 -0500 (EST) 9, 28 -- Received: from ([10.32.15.167]) 9, 28 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.4223090; 9, 28 -- Fri, 09 Jan 2009 15:20:15 -0500 9, 28 -- MIME-version: 1.0 9, 28 -- Content-transfer-encoding: 7BIT 9, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 9, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built 9, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KD800F280HR8880-at-umduwc01.umdnj.edu} for 9, 28 -- microscopy-at-microscopy.com; Fri, 09 Jan 2009 15:20:15 -0500 (EST) 9, 28 -- Message-id: {4967B1C9.50300-at-umdnj.edu} 9, 28 -- Date: Fri, 09 Jan 2009 15:21:29 -0500 9, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 9, 28 -- To: kamlennon-at-yahoo.com, microscopy-at-microscopy.com 9, 28 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy? 9, 28 -- References: {200901091958.n09Jw4H3015451-at-ns.microscopy.com} 9, 28 -- In-reply-to: {200901091958.n09Jw4H3015451-at-ns.microscopy.com} ==============================End of - Headers==============================
I have always found it strange that there is so much concern about the use of cacodylate in the EM laboratory. I concede that it is dangerous when handled incorrectly. However, I would think that phosphate buffer containing 2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution of osmium tetroxide. At what point should students start taking responsibility for handling chemicals safely and when do the trainers bite the bullet and make sure the training is adequate?
During my training I remember very clearly how I was taught how to handle pathogenic bacteria and viruses. The instructions were very clear and very strict, and followed the same basic rules of how I was trained to handle radioactive material. Basically the instructions were that these agents can make you sick and even kill you if you don't follow my instructions, so you have to handle them as follows....
When I moved into electron microscopy, the training I was given to prepare biological specimens consisted of being given access to a fridge full of chemicals and a written protocol. The brown spots on my hands appeared after a couple of hours but my corneas clouded over in about 30 min of my using the osmium tetroxide. I had been left completely unsupervised to handle these chemicals without any prior warning of their dangers. Interestingly, when my supervisor found out about my use of the osmium tetroxide, and what it had done to me, he blamed me!
With proper training, the chemicals we use in the EM lab are basically very safe. We use small amounts of them and store them in closed cabinets so they should not affect our health in any way.
Part of a good laboratory training course is teaching users how to handle toxic chemicals and how to pipette solutions without creating aerosols. Making sure that protective gloves are used correctly is another important aspect of this training.
If we train correctly, then it should be sufficient to warn students that they are handling materials designed to chemically alter biological material. At this point, I usually remind them that they are made of biological material too.
Happy New Year (My New Years Resolution is very high!)
Best regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009 16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KjYwS028110 16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 14:45:35 -0600 16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000 16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119 16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800 16, 18 -- Subject: Cac buffer and undergrads - chancy? 16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 16, 18 -- To: {Microscopy-at-Microscopy.Com} 16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org} 16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy? 16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw== 16, 18 -- Mime-version: 1.0 16, 18 -- Content-type: text/plain; 16, 18 -- charset="US-ASCII" 16, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
I naturally agree with Paul that with proper training that all the chemicals used in an EM are safe but that doesn't make it a good idea to use them indiscriminately. The comparison to bacteria and viruses is a red herring since those are typically the subject of interest as opposed to the selection of a buffer which is discretionary. Microscopists and other scientists inevitably generate hazardous waste but it is incumbent on us not to do so unless there is a scientific reason that it is the only way to do the experiment.
It is true that glutaraldehyde in phosphate buffer is dangerous. But glutaraldehyde in cacodylate buffer is more dangerous. Accidental exposure would mean exposure to two dangerous chemicals. In addition, adding acid to glutaraldehyde in phosphate will change the pH but not release arsenic gas. Many studies have shown the Good buffers to be suitable for LM and EM studies. I see no reason for any microscopist to cling to the use of cacodylate.
Paul's own horror story of poor training and supervision is further evidence of why one should minimize all unnecessary risks. I doubt any trained scientist would any chemical they don't fully understand outside of the hood and without gloves - that's a beginner's mistake. Unless one can guarantee being present at every step of a new student's processing, it would only be prudent to use the least toxic formulations. With experience, those students will be able to judge the risks they wish to take.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org] Sent: Friday, January 09, 2009 2:46 PM To: Phillips, Thomas E.
Dear All,
I have always found it strange that there is so much concern about the use of cacodylate in the EM laboratory. I concede that it is dangerous when handled incorrectly. However, I would think that phosphate buffer containing 2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution of osmium tetroxide. At what point should students start taking responsibility for handling chemicals safely and when do the trainers bite the bullet and make sure the training is adequate?
During my training I remember very clearly how I was taught how to handle pathogenic bacteria and viruses. The instructions were very clear and very strict, and followed the same basic rules of how I was trained to handle radioactive material. Basically the instructions were that these agents can make you sick and even kill you if you don't follow my instructions, so you have to handle them as follows....
When I moved into electron microscopy, the training I was given to prepare biological specimens consisted of being given access to a fridge full of chemicals and a written protocol. The brown spots on my hands appeared after a couple of hours but my corneas clouded over in about 30 min of my using the osmium tetroxide. I had been left completely unsupervised to handle these chemicals without any prior warning of their dangers. Interestingly, when my supervisor found out about my use of the osmium tetroxide, and what it had done to me, he blamed me!
With proper training, the chemicals we use in the EM lab are basically very safe. We use small amounts of them and store them in closed cabinets so they should not affect our health in any way.
Part of a good laboratory training course is teaching users how to handle toxic chemicals and how to pipette solutions without creating aerosols. Making sure that protective gloves are used correctly is another important aspect of this training.
If we train correctly, then it should be sufficient to warn students that they are handling materials designed to chemically alter biological material. At this point, I usually remind them that they are made of biological material too.
Happy New Year (My New Years Resolution is very high!)
Best regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
==============================Original Headers============================== 16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009 16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09KjYwS028110 16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 14:45:35 -0600 16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000 16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119 16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800 16, 18 -- Subject: Cac buffer and undergrads - chancy? 16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} 16, 18 -- To: {Microscopy-at-Microscopy.Com} 16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org} 16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy? 16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw== 16, 18 -- Mime-version: 1.0 16, 18 -- Content-type: text/plain; 16, 18 -- charset="US-ASCII" 16, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 29, 29 -- From PhillipsT-at-missouri.edu Fri Jan 9 15:18:40 2009 29, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 29, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09LIdVT010691 29, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:18:39 -0600 29, 29 -- X-IronPort-Anti-Spam-Filtered: true 29, 29 -- X-IronPort-Anti-Spam-Result: ApoEAGhNZ0nRauUp/2dsb2JhbADFJQEJhTmFVQGEP4Ev 29, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 29, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Jan 2009 15:18:38 -0600 29, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 29, 29 -- Fri, 9 Jan 2009 15:18:38 -0600 29, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 29, 29 -- Content-class: urn:content-classes:message 29, 29 -- MIME-Version: 1.0 29, 29 -- Content-Type: text/plain; 29, 29 -- charset="us-ascii" 29, 29 -- Subject: RE: [Microscopy] Cac buffer and undergrads - chancy? 29, 29 -- Date: Fri, 9 Jan 2009 15:18:37 -0600 29, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD640-at-UM-XMAIL06.um.umsystem.edu} 29, 29 -- In-Reply-To: {200901092046.n09KkNk7029228-at-ns.microscopy.com} 29, 29 -- X-MS-Has-Attach: 29, 29 -- X-MS-TNEF-Correlator: 29, 29 -- Thread-Topic: [Microscopy] Cac buffer and undergrads - chancy? 29, 29 -- thread-index: Aclym1aIby1FKakIQ163EmL7rOr/aAAArzOQ 29, 29 -- References: {200901092046.n09KkNk7029228-at-ns.microscopy.com} 29, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 29, 29 -- To: {PWebster-at-hei.org} , {Microscopy-at-microscopy.com} 29, 29 -- X-OriginalArrivalTime: 09 Jan 2009 21:18:38.0694 (UTC) FILETIME=[D6DCF060:01C9729F] 29, 29 -- Content-Transfer-Encoding: 8bit 29, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n09LIdVT010691 ==============================End of - Headers==============================
I disagree with the assumption that depleted uranium (DU) is not radioactive and the implication by the word "depleted" that all the radioactivity has been removed. Here's why.
I had 12 pounds of depleted UAc and a calibrated and certified "pancake" Geiger counter detector. It had no problem detecting background cosmic radiation and it had an up to date certification sticker on it. That amount of DUAc in those containers pegged my Geiger counter from three feet away. The one pound bottles were brown glass bottles, inside a plastic bag, inside a "tin" shipping can, and had labels that said, "(depleted uranium)" on them. So the counted radiation had to be gamma but U does not emit gamma radiation.
It is the impurities from the decay of U that generate the gamma emitters. I have no doubt that some of the posters think their materials are not radioactive and their supplier's material may not be. So we now have two schools of thought. It's not radioactive and there is radioactivity present. The only way to know for sure what you have and get a hint of the history of the manufacturing, is to take a reading on the purchased DUAc salt with a good quality Geiger counter and see what you get for a reading. My EH&S and safety people were totally shocked at the DUAc readings and said, "But this was made from depleted uranium. It's not radioactive." I relied, "Looks pretty radioactive to me." Like me, they believed the Geiger counter readings and not the MSDS sheets that didn't address the gamma emitting impurities, i.e. the amount of impurities from decay.
IMO, the phrase 'depleted uranium' is misleading. There is a shipping exemption on this radioactive material, I was told. If the amount is one ounce or less, you don't have to label it or ship it as radioactive. My shipping clerk refused to ship any amount. So just because the bottle or packaging you received does not say "radioactive material", that does not mean small amounts are not radioactive. I think that's where the EM myth of not being radioactive might comes in. How do you know every last U-235 atom was removed and the uranium was zone refined and/or chemically purified? You don't. It doesn't say any of that on the bottle(s). Just measure the gamma radiation.
Paul Beauregard Senior Research Associate
At 06:20 PM 1/8/09 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 28 -- From beaurega-at-westol.com Fri Jan 9 15:27:19 2009 8, 28 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.96.16]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09LRJTJ024332 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 15:27:19 -0600 8, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 8, 28 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id n09LREO4007130 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 16:27:15 -0500 8, 28 -- Received: (qmail 9427 invoked by uid 89); 9 Jan 2009 21:27:08 -0000 8, 28 -- Received: from pitts-69-72-21-71.dynamic-dialup.coretel.net (HELO running) (69.72.21.71) 8, 28 -- by mail.winbeam.com with SMTP; 9 Jan 2009 21:27:08 -0000 8, 28 -- Message-Id: {3.0.6.32.20090109162749.00893c10-at-pop3.norton.antivirus} 8, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 8, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 8, 28 -- Date: Fri, 09 Jan 2009 16:27:49 -0500 8, 28 -- To: microscopy-at-microscopy.com 8, 28 -- From: Beaurega {beaurega-at-westol.com} 8, 28 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters 8, 28 -- only 8, 28 -- Mime-Version: 1.0 8, 28 -- Content-Type: text/plain; charset="us-ascii" 8, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 8, 28 -- X-Winbeam-MailScanner-ID: n09LREO4007130 8, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 8, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 8, 28 -- SpamAssassin (not cached, score=-1.9, required 4, autolearn=not spam, 8, 28 -- AWL 0.10, BAYES_00 -2.00) 8, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com 8, 28 -- X-Winbeam-MailScanner-Watermark: 1232141236.05045-at-/cuIVPDK8713eRRY8mXR4w ==============================End of - Headers==============================
In our introductory and advanced courses we used phosphate buffers for animal tissue. My classmates and I all got knock-you-dead micrographs, better than the ones in our textbooks, of liver, kidney, muscle, brain, gills, lung, and etc., using phosphate buffers, so I think they can be used with animal tissue to obtain good results.
We're required to read the MSDS before using a chemical, and we're tested throughout both semesters on the safe handling of all EM chemicals, on ALL tests, even chemicals we don't used, from day one in lab.
On our first project, first semester, advanced students handled the OsO4 for us, and we made only the buffers and resins. Later in that semester we were handling and even preparing OsO4, and other chemicals, when we needed more or different concentrations, and if our teacher thought we could do so safely.
For our individual projects, later on, we can use the chemical fixation protocol of our choice--we write these up and submit them weeks before the project.
I used cacodylate buffer for my final project for my advanced biological EM course, and it had to be made from scratch. I worked with a partner, and we used a lab area when other students were absent, and had proper changes of gloves, fume hoods and scale arrangements that would not aerosolize anything, clean tools ready to limit handling time, etc., etc. We figured out the logistics and safe handling by ourselves from experience, questions, and the MSDS's.
We also had a great teacher who watched us like hawks before allowing us to mix our own chemicals and told us right away if we handled something incorrectly. Also, we watch and correct each other before our teacher gets the chance. Lab accidents caused by carelessness are a huge waste of time.
Kleo Pullin
--- On Fri, 1/9/09, kamlennon-at-yahoo.com {kamlennon-at-yahoo.com} wrote:
} From: kamlennon-at-yahoo.com {kamlennon-at-yahoo.com} } Subject: [Microscopy] Cac buffer and undergrads - chancy? } To: KLeoPullin-at-pacbell.net } Date: Friday, January 9, 2009, 12:01 PM } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Again Listers, } } Thanks to all of you who have responded to my recent query. } I'm sure that I will have more as time passes, and I } appreciate the fantastic response that I've had from the } list. } } Since we are on the topic of the dangers of EM work (the } uranyl acetate thread), I have a question about cacodylate } buffer. I'm getting set to teach an introductory EM } course for biologists to undergraduates. Having interviewed } two of my three students during the previous semester, I } know that I will be starting very much at zero. They had no } to little knowledge of what "EM" is or can be used } for before I spoke with them - they are exploring this new } class. My plan is to take them through the preparation of } plant, animal, and some sort of micro sample via traditional } chemical fixation methods and keep it as simple as possible. } I am inclined to steer clear of cacodylate buffer due to its } toxicity and because they have enough to deal with already, } and stick with phosphate buffer. However, I have noticed } that most if not all of the animal tissue protocols I've } been perusing use cac buffer. Is there any reason why I } should keep it in the protocol? } } Thanks for your advice. } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } } k.lennon-at-frostburg.edu } } } } } ==============================Original } Headers============================== } 7, 20 -- From kamlennon-at-yahoo.com Fri Jan 9 13:57:15 2009 } 7, 20 -- Received: from web84004.mail.mud.yahoo.com } (web84004.mail.mud.yahoo.com [68.142.206.174]) } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id n09JvEx0012745 } 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan } 2009 13:57:14 -0600 } 7, 20 -- Received: (qmail 76571 invoked by uid 60001); 9 } Jan 2009 19:57:14 -0000 } 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 7, 20 -- s=s1024; d=yahoo.com; } 7, 20 -- } h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; } 7, 20 -- } b=xeLr+fOkc5vJ3EXto1Y6+o7SUjRsZmyWr021p/AR8+eZSkJdh5uAKRIqv7XOXlDD/DxqNcT7zKQfKkLZ8xb+qim3FguZ95OneP3PcPPuzZm7XdHTVOeaRk/Onkfmz9gH67KG3edVJa6kzqjAXn/Wmi6J6hcnTSKOM2Iqc98V+Tg=; } 7, 20 -- X-YMail-OSG: } LFU8udYVM1kiLkk70s8A_YJn70xTwWxw7CGSMeW3AL1T.rnd4LZsOXO_lJUYOJekMg-- } 7, 20 -- Received: from [96.239.149.81] by } web84004.mail.mud.yahoo.com via HTTP; Fri, 09 Jan 2009 } 11:57:13 PST } 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 } 7, 20 -- Date: Fri, 9 Jan 2009 11:57:13 -0800 (PST) } 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } 7, 20 -- Reply-To: kamlennon-at-yahoo.com } 7, 20 -- Subject: Cac buffer and undergrads - chancy? } 7, 20 -- To: Microscopy-at-Microscopy.Com } 7, 20 -- MIME-Version: 1.0 } 7, 20 -- Content-Type: text/plain; charset=us-ascii } 7, 20 -- Message-ID: } {982974.76126.qm-at-web84004.mail.mud.yahoo.com} } ==============================End of - } Headers==============================
==============================Original Headers============================== 14, 20 -- From kleopullin-at-pacbell.net Fri Jan 9 15:52:21 2009 14, 20 -- Received: from web83405.mail.sp1.yahoo.com (web83405.mail.sp1.yahoo.com [69.147.64.53]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n09LqKsh006024 14, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 15:52:21 -0600 14, 20 -- Received: (qmail 85987 invoked by uid 60001); 9 Jan 2009 21:52:20 -0000 14, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 20 -- s=s1024; d=pacbell.net; 14, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 14, 20 -- b=6BGoEGZRiX+t35SsP/ZxdZwTKRcM/eFcrmMo1JyjALCkBsHqApT/5EZqJzjEZ5bCkyUsAPnUQiqztd2oHD+tITdKvXJOC5qBOOgAtzmogoO98XYNlm/vllX758+/6+v2picHm9h4SmFHDJVat4W9mbouXg6062oJnZOz2XHrmdI=; 14, 20 -- X-YMail-OSG: NdCCuD0VM1nODQ9p9gMVoVEdgF8YuA4Qjrm0TyceSJkdBxPoCeIqdeKIThmPxEhjBwfDH6WqSjV1gUcfyJ8GELMxiyC5xnNnEEKy.Oi_ywwTH5Kv92APyUsfSttvFbaPUeoaEqeZqghE8nvhJ0MxbZz9LbFZ4UuQsP_Kmh1pJOQq4F3zj28o_vhDTlka 14, 20 -- Received: from [69.225.11.246] by web83405.mail.sp1.yahoo.com via HTTP; Fri, 09 Jan 2009 13:52:20 PST 14, 20 -- X-Mailer: YahooMailWebService/0.7.218.2 14, 20 -- Date: Fri, 9 Jan 2009 13:52:20 -0800 (PST) 14, 20 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 14, 20 -- Reply-To: kleopullin-at-pacbell.net 14, 20 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy? 14, 20 -- To: Microscopy-at-Microscopy.Com 14, 20 -- MIME-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=us-ascii 14, 20 -- Message-ID: {479577.85874.qm-at-web83405.mail.sp1.yahoo.com} ==============================End of - Headers==============================
On Jan 9, 2009, at 1:27 PM, beaurega-at-westol.com wrote:
} I disagree with the assumption that depleted uranium (DU) is not } radioactive and the implication by the word "depleted" that all the } radioactivity has been removed.
Dear Paul, Depleted U is natural U from which almost all U235 has been removed. Just because U238 does not sustain a fission chain reaction does not mean that it is not radioactive. I would refer anyone who believes that U238 is not radioactive to any book or web site where radioactivity is discussed, or, as you say "Just measure the gamma radiation." Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Jan 9 16:14:35 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09MEZVe021473 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 16:14:35 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 36AD9328B97 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:14:35 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 40540328DC7 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 14:14:34 -0800 (PST) 6, 22 -- Message-Id: {ADAF5D19-031F-46B8-AA19-007568467A9C-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200901092127.n09LRPfo024462-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters 6, 22 -- Date: Fri, 9 Jan 2009 14:14:33 -0800 6, 22 -- References: {200901092127.n09LRPfo024462-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
My message about cacodylate was aimed at stimulating a continuation of this discussion, not to advocate the use of cacodylate buffer. However, the toxicity of the compound should not be the only reason for not choosing to use it. The choice of buffer will also depend on the end result needed.
My point in the first message was to point out that with proper training it is possible to handle almost any of the chemicals we encounter in a safe way.
One important caveat that I would add about using phosphate buffer in the primary fixative is that when mixed with glutaraldehyde and osmium tetroxide it can form a fine precipitate in the cells.
If using phosphate buffer, and there are plenty of historical references where it was used successfully for TEM, make sure the cells or tissues are washed well after glutaraldehyde fixation and before immersion in osmium tetroxide.
The choice of buffers for EM is another subject that deserves a long discussion thread. Tom Phillips suggests the use of Good buffers as an alternative to cacodylate. However, PIPES and HEPES will preserve tissues and cells in very different ways when compared with each other, as well as with cacodylate or phosphate buffers. This is partially due to the amount of extraction that occurs. In addition, PIPES buffer is often used at a pH where it has almost no buffering capacity, HEPES can preserve tissues so well that there is almost no extraction of cellular material, which results in virtually no specimen contrast and TRIS buffer may not work at all due to its ability to react with the aldehyde. In the end, fixation recipe changes should be approached with some caution.
Best regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } Date: Fri, 9 Jan 2009 15:18:37 -0600 } To: Paul Webster {PWebster-at-hei.org} , {Microscopy-at-microscopy.com} } Subject: RE: [Microscopy] Cac buffer and undergrads - chancy? } } I naturally agree with Paul that with proper training that all the } chemicals used in an EM are safe but that doesn't make it a good idea to } use them indiscriminately. The comparison to bacteria and viruses is a } red herring since those are typically the subject of interest as opposed } to the selection of a buffer which is discretionary. Microscopists and } other scientists inevitably generate hazardous waste but it is incumbent } on us not to do so unless there is a scientific reason that it is the } only way to do the experiment. } } It is true that glutaraldehyde in phosphate buffer is dangerous. But } glutaraldehyde in cacodylate buffer is more dangerous. Accidental } exposure would mean exposure to two dangerous chemicals. In addition, } adding acid to glutaraldehyde in phosphate will change the pH but not } release arsenic gas. Many studies have shown the Good buffers to be } suitable for LM and EM studies. I see no reason for any microscopist to } cling to the use of cacodylate. } } Paul's own horror story of poor training and supervision is further } evidence of why one should minimize all unnecessary risks. I doubt any } trained scientist would any chemical they don't fully understand outside } of the hood and without gloves - that's a beginner's mistake. Unless one } can guarantee being present at every step of a new student's processing, } it would only be prudent to use the least toxic formulations. With } experience, those students will be able to judge the risks they wish to } take. } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Chair, MU Faculty Council } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } -----Original Message----- } From: PWebster-at-hei.org [mailto:PWebster-at-hei.org] } Sent: Friday, January 09, 2009 2:46 PM } To: Phillips, Thomas E. } Subject: [Microscopy] Cac buffer and undergrads - chancy? } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Dear All, } } I have always found it strange that there is so much concern about the } use } of cacodylate in the EM laboratory. I concede that it is dangerous when } handled incorrectly. However, I would think that phosphate buffer } containing } 2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution of } osmium tetroxide. At what point should students start taking } responsibility } for handling chemicals safely and when do the trainers bite the bullet } and } make sure the training is adequate? } } During my training I remember very clearly how I was taught how to } handle } pathogenic bacteria and viruses. The instructions were very clear and } very } strict, and followed the same basic rules of how I was trained to handle } radioactive material. Basically the instructions were that these agents } can } make you sick and even kill you if you don't follow my instructions, so } you } have to handle them as follows.... } } When I moved into electron microscopy, the training I was given to } prepare } biological specimens consisted of being given access to a fridge full of } chemicals and a written protocol. The brown spots on my hands appeared } after } a couple of hours but my corneas clouded over in about 30 min of my } using } the osmium tetroxide. I had been left completely unsupervised to handle } these chemicals without any prior warning of their dangers. } Interestingly, } when my supervisor found out about my use of the osmium tetroxide, and } what } it had done to me, he blamed me! } } With proper training, the chemicals we use in the EM lab are basically } very } safe. We use small amounts of them and store them in closed cabinets so } they } should not affect our health in any way. } } Part of a good laboratory training course is teaching users how to } handle } toxic chemicals and how to pipette solutions without creating aerosols. } Making sure that protective gloves are used correctly is another } important } aspect of this training. } } If we train correctly, then it should be sufficient to warn students } that } they are handling materials designed to chemically alter biological } material. At this point, I usually remind them that they are made of } biological material too. } } Happy New Year } (My New Years Resolution is very high!) } } Best regards, } } Paul Webster. } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } } } } } } } ==============================Original } Headers============================== } 16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009 } 16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] } (may be forged)) } 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n09KjYwS028110 } 16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 } 14:45:35 -0600 } 16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by } hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV } ; } 16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000 } 16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119 } 16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800 } 16, 18 -- Subject: Cac buffer and undergrads - chancy? } 16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} } 16, 18 -- To: {Microscopy-at-Microscopy.Com} } 16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org} } 16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy? } 16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw== } 16, 18 -- Mime-version: 1.0 } 16, 18 -- Content-type: text/plain; } 16, 18 -- charset="US-ASCII" } 16, 18 -- Content-transfer-encoding: 7bit } ==============================End of - } Headers============================== }
==============================Original Headers============================== 12, 20 -- From PWebster-at-hei.org Fri Jan 9 17:15:16 2009 12, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09NFGaA004403 12, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 17:15:16 -0600 12, 20 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 12, 20 -- Fri, 9 Jan 2009 23:15:15 +0000 12, 20 -- User-Agent: Microsoft-Entourage/12.15.0.081119 12, 20 -- Date: Fri, 09 Jan 2009 15:15:11 -0800 12, 20 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy? 12, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 12, 20 -- To: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} , 12, 20 -- {Microscopy-at-microscopy.com} 12, 20 -- Message-ID: {C58D1A7F.1FB70%PWebster-at-hei.org} 12, 20 -- Thread-Topic: [Microscopy] Cac buffer and undergrads - chancy? 12, 20 -- Thread-Index: Aclym1aIby1FKakIQ163EmL7rOr/aAAArzOQAASC0Ww= 12, 20 -- In-Reply-To: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD640-at-UM-XMAIL06.um.umsystem.edu} 12, 20 -- Mime-version: 1.0 12, 20 -- Content-type: text/plain; 12, 20 -- charset="US-ASCII" 12, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
PIPES has a pKa of 6.8 which would make it a suitable buffer for 6.3 - 7.3 (and since aldehydes fixation tends to acidify the solution, it would be good at 7.4 also!). Cacodylate has a pKa of 6.2. I guess it depends on what pH you are using for your aldehydes fix but most people use something closer to 7 so I don't see how PIPES pKa is inferior to cacodylate.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: Webster, Paul [mailto:PWebster-at-hei.org] Sent: Friday, January 09, 2009 5:15 PM To: Phillips, Thomas E.; Microscopy-at-microscopy.com
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Email: rp_seifi-at-yahoo.com Name: Reza Pourseify
Organization: Mehr Inst.
Title-Subject: [Filtered] FISH
Question: Hello everybody I have a problem in my microscope setting. The line of light is biased to lefthand side in it for some zoom degrees. How can i correct it. It is a Nikon, E600 (fluorescence microscope with visible light harware and a condenser) Thank you for attention...
One last time - I am not advocating the use of cacodylate and I do agree that cacodylate as generally used in EM has less buffering capacity than PIPES. I don't think I ever said that one buffer was inferior to another, just that choices should be made on the end result, not on whether a compound was toxic or not.
Regards,
Paul Webster.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } Date: Fri, 9 Jan 2009 17:22:17 -0600 } To: Paul Webster {PWebster-at-hei.org} , {Microscopy-at-microscopy.com} } Subject: RE: [Microscopy] Cac buffer and undergrads - chancy? } } PIPES has a pKa of 6.8 which would make it a suitable buffer for 6.3 - } 7.3 (and since aldehydes fixation tends to acidify the solution, it } would be good at 7.4 also!). Cacodylate has a pKa of 6.2. I guess it } depends on what pH you are using for your aldehydes fix but most people } use something closer to 7 so I don't see how PIPES pKa is inferior to } cacodylate. } } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Chair, MU Faculty Council } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } -----Original Message----- } From: Webster, Paul [mailto:PWebster-at-hei.org] } Sent: Friday, January 09, 2009 5:15 PM } To: Phillips, Thomas E.; Microscopy-at-microscopy.com } Subject: Re: [Microscopy] Cac buffer and undergrads - chancy? } } Hi All, } } My message about cacodylate was aimed at stimulating a continuation of } this } discussion, not to advocate the use of cacodylate buffer. However, the } toxicity of the compound should not be the only reason for not choosing } to } use it. The choice of buffer will also depend on the end result needed. } } My point in the first message was to point out that with proper training } it } is possible to handle almost any of the chemicals we encounter in a safe } way. } } One important caveat that I would add about using phosphate buffer in } the } primary fixative is that when mixed with glutaraldehyde and osmium } tetroxide } it can form a fine precipitate in the cells. } } If using phosphate buffer, and there are plenty of historical references } where it was used successfully for TEM, make sure the cells or tissues } are } washed well after glutaraldehyde fixation and before immersion in osmium } tetroxide. } } The choice of buffers for EM is another subject that deserves a long } discussion thread. Tom Phillips suggests the use of Good buffers as an } alternative to cacodylate. However, PIPES and HEPES will preserve } tissues } and cells in very different ways when compared with each other, as well } as } with cacodylate or phosphate buffers. This is partially due to the } amount of } extraction that occurs. In addition, PIPES buffer is often used at a pH } where it has almost no buffering capacity, HEPES can preserve tissues so } well that there is almost no extraction of cellular material, which } results } in virtually no specimen contrast and TRIS buffer may not work at all } due to } its ability to react with the aldehyde. In the end, fixation recipe } changes } should be approached with some caution. } } Best regards, } } Paul. } } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } } } From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } } Date: Fri, 9 Jan 2009 15:18:37 -0600 } } To: Paul Webster {PWebster-at-hei.org} , {Microscopy-at-microscopy.com} } } Subject: RE: [Microscopy] Cac buffer and undergrads - chancy? } } } } I naturally agree with Paul that with proper training that all the } } chemicals used in an EM are safe but that doesn't make it a good idea } to } } use them indiscriminately. The comparison to bacteria and viruses is a } } red herring since those are typically the subject of interest as } opposed } } to the selection of a buffer which is discretionary. Microscopists and } } other scientists inevitably generate hazardous waste but it is } incumbent } } on us not to do so unless there is a scientific reason that it is the } } only way to do the experiment. } } } } It is true that glutaraldehyde in phosphate buffer is dangerous. But } } glutaraldehyde in cacodylate buffer is more dangerous. Accidental } } exposure would mean exposure to two dangerous chemicals. In addition, } } adding acid to glutaraldehyde in phosphate will change the pH but not } } release arsenic gas. Many studies have shown the Good buffers to be } } suitable for LM and EM studies. I see no reason for any microscopist } to } } cling to the use of cacodylate. } } } } Paul's own horror story of poor training and supervision is further } } evidence of why one should minimize all unnecessary risks. I doubt any } } trained scientist would any chemical they don't fully understand } outside } } of the hood and without gloves - that's a beginner's mistake. Unless } one } } can guarantee being present at every step of a new student's } processing, } } it would only be prudent to use the least toxic formulations. With } } experience, those students will be able to judge the risks they wish } to } } take. } } } } } } Thomas E. Phillips, Ph.D } } Professor of Biological Sciences } } Chair, MU Faculty Council } } Director, Molecular Cytology Core } } 2 Tucker Hall } } University of Missouri } } Columbia, MO 65211-7400 } } 573-882-4712 (office) } } 573-882-0123 (fax) } } phillipst-at-missouri.edu } } } } http://www.biology.missouri.edu/faculty/phillips.html } } http://www.biotech.missouri.edu/mcc/ } } } } -----Original Message----- } } From: PWebster-at-hei.org [mailto:PWebster-at-hei.org] } } Sent: Friday, January 09, 2009 2:46 PM } } To: Phillips, Thomas E. } } Subject: [Microscopy] Cac buffer and undergrads - chancy? } } } } } } } } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } } ---- } } } } Dear All, } } } } I have always found it strange that there is so much concern about the } } use } } of cacodylate in the EM laboratory. I concede that it is dangerous } when } } handled incorrectly. However, I would think that phosphate buffer } } containing } } 2.5% glutaraldehyde is more dangerous, or even a 2% aqueous solution } of } } osmium tetroxide. At what point should students start taking } } responsibility } } for handling chemicals safely and when do the trainers bite the bullet } } and } } make sure the training is adequate? } } } } During my training I remember very clearly how I was taught how to } } handle } } pathogenic bacteria and viruses. The instructions were very clear and } } very } } strict, and followed the same basic rules of how I was trained to } handle } } radioactive material. Basically the instructions were that these } agents } } can } } make you sick and even kill you if you don't follow my instructions, } so } } you } } have to handle them as follows.... } } } } When I moved into electron microscopy, the training I was given to } } prepare } } biological specimens consisted of being given access to a fridge full } of } } chemicals and a written protocol. The brown spots on my hands appeared } } after } } a couple of hours but my corneas clouded over in about 30 min of my } } using } } the osmium tetroxide. I had been left completely unsupervised to } handle } } these chemicals without any prior warning of their dangers. } } Interestingly, } } when my supervisor found out about my use of the osmium tetroxide, and } } what } } it had done to me, he blamed me! } } } } With proper training, the chemicals we use in the EM lab are basically } } very } } safe. We use small amounts of them and store them in closed cabinets } so } } they } } should not affect our health in any way. } } } } Part of a good laboratory training course is teaching users how to } } handle } } toxic chemicals and how to pipette solutions without creating } aerosols. } } Making sure that protective gloves are used correctly is another } } important } } aspect of this training. } } } } If we train correctly, then it should be sufficient to warn students } } that } } they are handling materials designed to chemically alter biological } } material. At this point, I usually remind them that they are made of } } biological material too. } } } } Happy New Year } } (My New Years Resolution is very high!) } } } } Best regards, } } } } Paul Webster. } } } } Paul Webster, Ph.D } } House Ear Institute } } 2100 West Third Street } } Los Angeles, CA 90057 } } (213) 273 8026 } } pwebster-at-hei.org } } } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } 16, 18 -- From PWebster-at-hei.org Fri Jan 9 14:45:35 2009 } } 16, 18 -- Received: from hi0sml1.hei.org (heimail.hei.org } [12.88.48.54] } } (may be forged)) } } 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id n09KjYwS028110 } } 16, 18 -- for {Microscopy-at-Microscopy.Com} ; Fri, 9 Jan 2009 } } 14:45:35 -0600 } } 16, 18 -- Received: from 10.10.42.117 ([10.10.42.117]) by } } hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server } HTTP-DAV } } ; } } 16, 18 -- Fri, 9 Jan 2009 20:45:34 +0000 } } 16, 18 -- User-Agent: Microsoft-Entourage/12.15.0.081119 } } 16, 18 -- Date: Fri, 09 Jan 2009 12:45:30 -0800 } } 16, 18 -- Subject: Cac buffer and undergrads - chancy? } } 16, 18 -- From: "Webster, Paul" {PWebster-at-hei.org} } } 16, 18 -- To: {Microscopy-at-Microscopy.Com} } } 16, 18 -- Message-ID: {C58CF76A.1FB48%PWebster-at-hei.org} } } 16, 18 -- Thread-Topic: Cac buffer and undergrads - chancy? } } 16, 18 -- Thread-Index: AclymzWCIWUJgAPwQOO74u5GyMglfw== } } 16, 18 -- Mime-version: 1.0 } } 16, 18 -- Content-type: text/plain; } } 16, 18 -- charset="US-ASCII" } } 16, 18 -- Content-transfer-encoding: 7bit } } ==============================End of - } } Headers============================== } } }
==============================Original Headers============================== 9, 19 -- From PWebster-at-hei.org Fri Jan 9 17:38:47 2009 9, 19 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n09NckbP012473 9, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 17:38:47 -0600 9, 19 -- Received: from 10.10.42.117 ([10.10.42.117]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 9, 19 -- Fri, 9 Jan 2009 23:38:46 +0000 9, 19 -- User-Agent: Microsoft-Entourage/12.15.0.081119 9, 19 -- Date: Fri, 09 Jan 2009 15:38:44 -0800 9, 19 -- Subject: Re: [Microscopy] Cac buffer and undergrads - chancy? 9, 19 -- From: "Webster, Paul" {PWebster-at-hei.org} 9, 19 -- To: {Microscopy-at-microscopy.com} 9, 19 -- Message-ID: {C58D2004.1FB7C%PWebster-at-hei.org} 9, 19 -- Thread-Topic: [Microscopy] Cac buffer and undergrads - chancy? 9, 19 -- Thread-Index: Aclym1aIby1FKakIQ163EmL7rOr/aAAArzOQAASC0WwAACdVkAAAqzm5 9, 19 -- In-Reply-To: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD675-at-UM-XMAIL06.um.umsystem.edu} 9, 19 -- Mime-version: 1.0 9, 19 -- Content-type: text/plain; 9, 19 -- charset="US-ASCII" 9, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Yes, I remember all this being discussed by a few of us, including you, back a few years ago. One guy essentially called my certified Geiger counter a liar. Like I saw in the past, I keep seeing web pages (EPA and Health Canada, for example) that say that DU is still 0.2-0.4 % U-235 versus the original 0.7%. I always remember that it is roughly only half to 70% depleted. My observation of the phenomena.
Someone strongly pointed out that U is an alpha emitter, not a gamma emitter, and I was not reading gamma radiation (but I was). You pointed out that it was the decay impurities that were the gamma emitters and that was what I was reading on my counter. I agree that U-238 and DU are still radioactive.
http://www.hc-sc.gc.ca/ewh-semt/pubs/radiation/uranium-eng.php "It (uranium) consists of three isotopes: uranium-234, uranium-235 and uranium-238 which are present in amounts of 0.005%, 0.7%, and 99.3%, by weight, respectively." "The amounts of uranium-234 and uranium-235 remaining in depleted uranium metal are about 0.002% and 0.2%, respectively." Here's my favorite. "depleted uranium is 40% less radioactive than natural uranium." That means 60% of the radioactivity is still there. I am not sure that includes gamma but probably.
My main point this time around was that 'whatever was in all my bottles of DUAc', it was also a gamma emitter. Also, it is impossible to know the history of the manufacturing of the material and so one should take their own Geiger counter readings to see what gamma radiation is actually there. That's the final referee on the raw salt, IMO. It's also a measure of the impurities, I guess. Of course the dilution level makes the gamma levels less in the final solutions used in EM.
JMO,
Paul
At 04:14 PM 1/9/09 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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One more consideration is the logistics of it all: are the students early college and new to science or advanced and already well on a science path? I say, save the hazardous stuff for the latter group, generally. Let them get hooked before you trot out the dangers! ;-) Also: class size matters a lot, and how much other stuff you have to fit into the class session (hazardous stuff needs a bit of time). I've found that most students will be fine, but there's always someone who wasn't paying attention and isn't careful, so you need a small class and a good lab setup (dangerous stuff not too close to other stuff). In one class (intro level) we were doing blood draws (for blood typing) and I was very surprised at how careless they were (especially when they were distracted by results), so I'd call them up in small groups, but this took a while and some of them still managed to get blood where it shouldn't have been. (Nothing serious, but it kept me on my toes a bit too much for comfort.)
In training my students in microscopy, I try to impart good habits, figuring, better they should start at the "taking extreme care level" then when their habits inevitably decay, they'll still have decent enough habits. It's funny when I forget myself to follow the rules (always koehler, no food near scopes, start on low power). Just the other day my own tech glared at me when I walked into the scope room, and it took me a sec, but i realized that i was holding (and eating) very crumbly food.
On the other extreme, we once had a stockroom tech here who was very afraid of all chemicals, despite her degree in biology. She told me (I have this in email, so I wasn't hallucinating it) that she couldn't prepare a NaCl solution for me because she hadn't had safety training yet. Really!
Gisele
==============================Original Headers============================== 6, 27 -- From tiger3g3-at-yahoo.com Fri Jan 9 22:27:25 2009 6, 27 -- Received: from n17.bullet.mail.mud.yahoo.com (n17.bullet.mail.mud.yahoo.com [68.142.206.144]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0A4RPM1014323 6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 22:27:25 -0600 6, 27 -- Received: from [68.142.194.244] by n17.bullet.mail.mud.yahoo.com with NNFMP; 10 Jan 2009 04:27:25 -0000 6, 27 -- Received: from [68.142.201.66] by t2.bullet.mud.yahoo.com with NNFMP; 10 Jan 2009 04:27:25 -0000 6, 27 -- Received: from [127.0.0.1] by omp418.mail.mud.yahoo.com with NNFMP; 10 Jan 2009 04:27:25 -0000 6, 27 -- X-Yahoo-Newman-Id: 144280.94259.bm-at-omp418.mail.mud.yahoo.com 6, 27 -- Received: (qmail 10754 invoked from network); 10 Jan 2009 04:27:24 -0000 6, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 27 -- s=s1024; d=yahoo.com; 6, 27 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:Mime-Version:X-Sender:Message-Id:In-Reply-To:References:Date:To:From:Subject:Content-Type; 6, 27 -- b=GASZ7cSffEmx323Qb16TrqtdbcP/SnEoR0vEf8//n0B03XAdsDlaKb1HXQ89y9cHb9XRhu8tpYLNUtp1AESktNO8NmwSNyzIkQGyZJGlo7D4TtB5Qk52/jh344F4Au7M1TuRWnIfVkFK2eN0/eNbBqeoXRvLbYubV5NZSV+cKn4= ; 6, 27 -- Received: from unknown (HELO ?98.207.93.115?) (tiger3g3-at-98.207.93.115 with plain) 6, 27 -- by smtp105.plus.mail.sp1.yahoo.com with SMTP; 10 Jan 2009 04:27:24 -0000 6, 27 -- X-YMail-OSG: e7IKwhcVM1l3xBi7aKLaR11lkO5AIcUMmtp5Ce6HYsv2vUvu50c1nRr4X.4sh_tcEIe_bIfMmsW_CxDFCpdpTGN0KwsWDLNEGGPChjfh476nlZsYepenCBKGQzGosezMyVCtpuoGQ8d5N9JFOL_rV.2XvycnRs91p4HPpEQGVrEt45Gi1T6yjLA4oZo- 6, 27 -- X-Yahoo-Newman-Property: ymail-3 6, 27 -- Mime-Version: 1.0 6, 27 -- X-Sender: tiger3g3-at-pop.mail.yahoo.com 6, 27 -- Message-Id: {a05111b44c58dd02ea52e-at-[98.207.93.115]} 6, 27 -- In-Reply-To: {200901092123.n09LNTVK021406-at-ns.microscopy.com} 6, 27 -- References: {200901092123.n09LNTVK021406-at-ns.microscopy.com} 6, 27 -- Date: Fri, 9 Jan 2009 20:27:23 -0800 6, 27 -- To: microscopy-at-microscopy.com 6, 27 -- From: Gisele Eliane Giorgi {tiger3g3-at-yahoo.com} 6, 27 -- Subject: [Microscopy] RE: Cac buffer and undergrads - chancy? 6, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Listers - I have samples to prepare for inspection by SEM, and the client wants to know about the grain size. We have never polished metals before. Is there anyone who can advise me what to do, or does this take a metallurgist? Carol Heckman, Bowling Green State University
==============================Original Headers============================== 1, 19 -- From heckman-at-buckeye-express.com Sat Jan 10 00:50:38 2009 1, 19 -- Received: from omta0106.mta.everyone.net (imta-38.everyone.net [216.200.145.38]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0A6ocXh001891 1, 19 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 00:50:38 -0600 1, 19 -- Received: from dm24.mta.everyone.net (sj1-slb03-gw2 [172.16.1.96]) 1, 19 -- by omta0106.mta.everyone.net (Postfix) with ESMTP id 14B4072C3DD 1, 19 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 22:51:05 -0800 (PST) 1, 19 -- X-Eon-Dm: dm24 1, 19 -- Received: by resin15.mta.everyone.net (EON-PICKUP) 1, 19 -- id resin15.4962afe0.2912b; Fri, 9 Jan 2009 22:50:37 -0800 1, 19 -- MIME-Version: 1.0 1, 19 -- Content-Type: text/plain; charset="UTF-8" 1, 19 -- Message-Id: {20090109225037.A97AB905-at-resin15.mta.everyone.net} 1, 19 -- Date: Fri, 9 Jan 2009 22:50:37 -0800 1, 19 -- From: {heckman-at-buckeye-express.com} 1, 19 -- To: {microscopy-at-microscopy.com} 1, 19 -- Subject: polishing stainless steel and silver metal surfaces 1, 19 -- X-Eon-Sig: AQK8SgtJaEU99JcqNgEAAAAB,8565da5615434e3c9ae0f09b36273f8f 1, 19 -- X-Originating-Ip: [72.241.42.208] ==============================End of - Headers==============================
You might be better off delegating this one to somebody in the materials science department, but if you want to try it yourself, I can help you with the silver. You didn't mention what silver alloy you're attempting to prepare, or it's form, but here's a method that works well for preparing fine and sterling silver, yellow and white gold alloys, nickel, copper alloys, and silver solders on rotating wheels at 400 rpm. The type of lubricant, cloth selection, and pressure are important.
1) Grind through a succession of SiC papers from 240 through 600 grit using moderate pressure with running water lubricant. Rinse or ultrasonically clean after each paper. Dry. 2) Coarse polish on a napless cotton cloth with 6 micron diamond (I use a suspension), and moderate pressure with a commercial diamond extender. Ultrasonically clean and dry. 3) Fine polish on a short-napped synthetic velvet cloth with 1 micron diamond (I use a suspension) and deionized water lubricant using moderate to light pressure. Ultrasonically clean and dry.
You'll have to etch the silver to reveal the grain size after polishing. Without knowing what silver alloy you're working with, try a 50/50 mixture of ammonium hydroxide and hydrogen peroxide (30% concentration). The etch works very fast. It can be diluted with DI water to slow the attack. Apply by swabbing. Do not store.
Good luck,
Jeff Stewart Metallographic Lab Manager Stern-Leach Company Cookson Precious Metals 49 Pearl Street Attleboro, MA 02703 508-222-7400 x1329
-----Original Message----- X-from: heckman-at-buckeye-express.com [mailto:heckman-at-buckeye-express.com] Sent: Saturday, January 10, 2009 1:58 AM To: jeff-at-metallography.com
Listers - I have samples to prepare for inspection by SEM, and the client wants to know about the grain size. We have never polished metals before. Is there anyone who can advise me what to do, or does this take a metallurgist? Carol Heckman, Bowling Green State University
==============================Original Headers============================== 1, 19 -- From heckman-at-buckeye-express.com Sat Jan 10 00:50:38 2009 1, 19 -- Received: from omta0106.mta.everyone.net (imta-38.everyone.net [216.200.145.38]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0A6ocXh001891 1, 19 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 00:50:38 -0600 1, 19 -- Received: from dm24.mta.everyone.net (sj1-slb03-gw2 [172.16.1.96]) 1, 19 -- by omta0106.mta.everyone.net (Postfix) with ESMTP id 14B4072C3DD 1, 19 -- for {microscopy-at-microscopy.com} ; Fri, 9 Jan 2009 22:51:05 -0800 (PST) 1, 19 -- X-Eon-Dm: dm24 1, 19 -- Received: by resin15.mta.everyone.net (EON-PICKUP) 1, 19 -- id resin15.4962afe0.2912b; Fri, 9 Jan 2009 22:50:37 -0800 1, 19 -- MIME-Version: 1.0 1, 19 -- Content-Type: text/plain; charset="UTF-8" 1, 19 -- Message-Id: {20090109225037.A97AB905-at-resin15.mta.everyone.net} 1, 19 -- Date: Fri, 9 Jan 2009 22:50:37 -0800 1, 19 -- From: {heckman-at-buckeye-express.com} 1, 19 -- To: {microscopy-at-microscopy.com} 1, 19 -- Subject: polishing stainless steel and silver metal surfaces 1, 19 -- X-Eon-Sig: AQK8SgtJaEU99JcqNgEAAAAB,8565da5615434e3c9ae0f09b36273f8f 1, 19 -- X-Originating-Ip: [72.241.42.208] ==============================End of - Headers============================== Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.176 / Virus Database: 270.9.13/1825 - Release Date: 12/2/2008 8:44 PM
==============================Original Headers============================== 12, 39 -- From SRS0=BqBZVq=5O=metallography.com=jeff-at-eigbox.net Sat Jan 10 07:13:48 2009 12, 39 -- Received: from bosmailout13.eigbox.net (bosmailout13.eigbox.net [66.96.190.13]) 12, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0ADDm4T030867 12, 39 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 07:13:48 -0600 12, 39 -- Message-Id: {200901101313.n0ADDm4T030867-at-ns.microscopy.com} 12, 39 -- Received: from bosmailscan16.eigbox.net ([10.20.15.16]) 12, 39 -- by bosmailout13.eigbox.net with esmtp (Exim) 12, 39 -- id 1LLdeu-0005st-5e 12, 39 -- for microscopy-at-microscopy.com; Sat, 10 Jan 2009 08:13:48 -0500 12, 39 -- Received: from bosimpout01.eigbox.net ([10.20.55.1]) 12, 39 -- by bosmailscan16.eigbox.net with esmtp (Exim) 12, 39 -- id 1LLdeu-0001p7-3n 12, 39 -- for microscopy-at-microscopy.com; Sat, 10 Jan 2009 08:13:48 -0500 12, 39 -- Received: from bosauthsmtp04.eigbox.net ([10.20.18.4]) 12, 39 -- by bosimpout01.eigbox.net with NO UCE 12, 39 -- id 1kzR1b00205GATN0000000; Sat, 10 Jan 2009 03:59:25 -0500 12, 39 -- X-EN-OrigOutIP: 10.20.18.4 12, 39 -- X-EN-IMPSID: 1kzR1b00205GATN0000000 12, 39 -- Received: from pool-71-184-39-246.bstnma.east.verizon.net ([71.184.39.246] helo=Jeff) 12, 39 -- by bosauthsmtp04.eigbox.net with esmtpa (Exim) 12, 39 -- id 1LLdei-0004z3-GC 12, 39 -- for Microscopy-at-microscopy.com; Sat, 10 Jan 2009 08:13:37 -0500 12, 39 -- From: "Jeff Stewart" {jeff-at-metallography.com} 12, 39 -- To: {Microscopy-at-microscopy.com} 12, 39 -- Subject: RE: [Microscopy] polishing stainless steel and silver metal surfaces 12, 39 -- Date: Sat, 10 Jan 2009 08:13:26 -0500 12, 39 -- MIME-Version: 1.0 12, 39 -- Content-Type: text/plain; 12, 39 -- charset="us-ascii" 12, 39 -- Content-Transfer-Encoding: 7bit 12, 39 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 12, 39 -- Thread-Index: Acly8LYf6PtvJgQ4TkOQ3DxLbWpvMgAL34gg 12, 39 -- In-Reply-To: {200901100657.n0A6vW0N015262-at-ns.microscopy.com} 12, 39 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 12, 39 -- X-EN-UserInfo: e86f17d72db2b073c5072c937c9a2a3f:f6ee1f837e2a1583e399b28f5cf1cc41 12, 39 -- X-EN-AuthUser: jeff-at-metallography.com 12, 39 -- Sender: "Jeff Stewart" {jeff-at-metallography.com} 12, 39 -- X-EN-OrigIP: 71.184.39.246 12, 39 -- X-EN-OrigHost: pool-71-184-39-246.bstnma.east.verizon.net ==============================End of - Headers==============================
If you are looking to measure the grain size of the stainless steel, refer to ASTM E112 for the measurement procedure. You will need to properly polish and etch first. The alloy will dictate the proper etchant to use. Try Viella's for 400 series. For 300 series, try Kalling's II, Glyceregia or electrolytic 10% oxalic.
Do not store glyceregia!
Alan Stone ASTON
At 12:51 AM 1/10/2009, heckman-at-buckeye-express.com wrote:
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Alan Stone ASTON Metallurgical Services Co., Inc.
This confidential message is for the private use of the recipient as shown. If this has been delivered to you in error, then please return the message and delete from your files. Thank you.
==============================Original Headers============================== 12, 22 -- From as-at-astonmet.com Sat Jan 10 09:26:00 2009 12, 22 -- Received: from outbound2.mail.tds.net (outbound2.mail.tds.net [216.170.230.92]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AFQ07a015853 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 09:26:00 -0600 12, 22 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32]) 12, 22 -- by outbound2.mail.tds.net (8.13.6/8.13.4) with ESMTP id n0AFQ0PY000412 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 09:26:00 -0600 12, 22 -- Received: from mozart.astonmet.com ([69.11.219.4]) 12, 22 -- by outaamta02.mail.tds.net with ESMTP 12, 22 -- id {20090110152559.DTZC4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 09:25:59 -0600 12, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 22 -- Date: Sat, 10 Jan 2009 09:25:58 -0600 12, 22 -- To: microscopy-at-microscopy.com 12, 22 -- From: Alan Stone {as-at-astonmet.com} 12, 22 -- Subject: Re: [Microscopy] polishing stainless steel and silver metal 12, 22 -- surfaces 12, 22 -- In-Reply-To: {200901100651.n0A6pis2003576-at-ns.microscopy.com} 12, 22 -- References: {200901100651.n0A6pis2003576-at-ns.microscopy.com} 12, 22 -- Mime-Version: 1.0 12, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 12, 22 -- Message-Id: {20090110152559.DTZC4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} ==============================End of - Headers==============================
I'm sorry about sounding a bit negative in my previous post. I did not mean to school anybody.
I just wanted to clarify that the radioactive decay process of U-238 continues via further decay of daughter nuclei with different modes of decay. And people can do their own calculations, as well.
Regards, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Fri, Jan 9, 2009 at 4:44 PM, {dac-at-research.umass.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Ayten, } } Your comments are informative but, to my personal taste, definitely a } bit negative in tone for this list. Your "schooling" of Alex isn't } really necessary and I think we could get your information in a more } positive and collegial way. } } Regarding the decay tree, I note from the provided link information that } the half-life of U-238 is { {4.468E+9 years} } . It has been a while since } my high school chemistry but I'm wondering how much Th-234 and } associated beta emission danger we are really dealing with here; seems } like there must be a very small amount of Th-234 produced with such a } long half-life of the original U-238. Maybe you could comment on the } danger of this. } } Thanks, } } Dale } } } celikaktas-at-gmail.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Alex, } } } } Did you have a chance to check the information from } } } } http://atom.kaeri.re.kr/ton/nuc7.html } } } } Let me construct the decay tree. I'm sure everybody in this list can } } do it but, you keep insisting that "uranyl compounds are alpha } } emitters only" so, I will take the time to do the job and post in to } } the list. } } } } Let's start with U-238 which is the starting element in your compound. } } } } 1) U-238 decays into Th-234 by Alpha decay } } } } 2) Th-234 decays into Pa-234 by Beta decay } } } } 3) Pa-234 decays into U-234 by Beta decay } } } } 4) U-234 decays into Th-230 by Alpha decay } } } } 5) Th-230 decays into Ra-226 by Alpha decay } } } } 6) Ra-226 decays into Rn-222 by Alpha decay } } } } 7) Rn-222 decays into Po-218 by Alpha decay } } } } 8) Po-218 decays into Pb-214 by Alpha decay } } } } 9) Pb-214 decays into Bi-214 by Beta decay } } } } 10) Bi-214 decays into Po-214 by Beta decay } } } } 11) Po-214 decays into Pb-210 by Alpha decay } } } } 12) Pb-210 decays into Bi-210 by Beta decay } } } } 13) Bi-210 decays into Po-210 by Beta decay } } } } 14) Po-210 decays into Pb-206 by Alpha decay } } } } Pb-206 is STABLE so, it is the last element to be produced as a result } } of U-238 radioactive decay. } } } } I have constructed the above decay tree using the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } While constructing the above decay tree I have used the branch which } } has the highest branch ratio (above 99% in each case). } } } } I do not understand why you are trying to keep things "under control"? } } } } By the way, I'm a Nuclear Engineer. } } } } One does not even need to be nuclear engineer to understand this. Even } } in high school science classes people learn about radioactive decay } } series e.g. A decays into B and B decays into C... } } } } Best, } } Ayten. } } } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } ===========================
Based on Dale's reported half life for U238, the specific activity for U238 would be 1.2x10exp4 DPS, or about 0.3 uCi/g. This is very small activity and they're alpha particles, which can be shielded best by plastic to avoid Bremstrahlung and secondary x-ray production. The daughter products will accumulate and decay according to their schemes and half-lives, which makes it more difficult to calculate. (See "Nuclear and Radiochemistry", by Friedlander, Kennedy, Miller, for a treatise on the differential equations to calculate activity from multiple decay pathways.)
The best is to just measure it with a survey meter and see what the external total dose rate in mr/hr is. Don't be afraid if your meter 'pegs' on the lowest (most sensitive setting). A sample with a field over several mr/hr over background should be handled according to practices used for handling radioactive materials.
Hope that helps a bit.
Don Kloos VP Sales, Marketing, Business Development Parallax Research
-----Original Message----- X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] Sent: Saturday, January 10, 2009 8:59 AM To: dkloos-at-parallaxray.com
Dear Listers,
I'm sorry about sounding a bit negative in my previous post. I did not mean to school anybody.
I just wanted to clarify that the radioactive decay process of U-238 continues via further decay of daughter nuclei with different modes of decay. And people can do their own calculations, as well.
Regards, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Fri, Jan 9, 2009 at 4:44 PM, {dac-at-research.umass.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Ayten, } } Your comments are informative but, to my personal taste, definitely a } bit negative in tone for this list. Your "schooling" of Alex isn't } really necessary and I think we could get your information in a more } positive and collegial way. } } Regarding the decay tree, I note from the provided link information that } the half-life of U-238 is { {4.468E+9 years} } . It has been a while since } my high school chemistry but I'm wondering how much Th-234 and } associated beta emission danger we are really dealing with here; seems } like there must be a very small amount of Th-234 produced with such a } long half-life of the original U-238. Maybe you could comment on the } danger of this. } } Thanks, } } Dale } } } celikaktas-at-gmail.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Alex, } } } } Did you have a chance to check the information from } } } } http://atom.kaeri.re.kr/ton/nuc7.html } } } } Let me construct the decay tree. I'm sure everybody in this list can } } do it but, you keep insisting that "uranyl compounds are alpha } } emitters only" so, I will take the time to do the job and post in to } } the list. } } } } Let's start with U-238 which is the starting element in your compound. } } } } 1) U-238 decays into Th-234 by Alpha decay } } } } 2) Th-234 decays into Pa-234 by Beta decay } } } } 3) Pa-234 decays into U-234 by Beta decay } } } } 4) U-234 decays into Th-230 by Alpha decay } } } } 5) Th-230 decays into Ra-226 by Alpha decay } } } } 6) Ra-226 decays into Rn-222 by Alpha decay } } } } 7) Rn-222 decays into Po-218 by Alpha decay } } } } 8) Po-218 decays into Pb-214 by Alpha decay } } } } 9) Pb-214 decays into Bi-214 by Beta decay } } } } 10) Bi-214 decays into Po-214 by Beta decay } } } } 11) Po-214 decays into Pb-210 by Alpha decay } } } } 12) Pb-210 decays into Bi-210 by Beta decay } } } } 13) Bi-210 decays into Po-210 by Beta decay } } } } 14) Po-210 decays into Pb-206 by Alpha decay } } } } Pb-206 is STABLE so, it is the last element to be produced as a result } } of U-238 radioactive decay. } } } } I have constructed the above decay tree using the information from } } http://atom.kaeri.re.kr/ton/nuc7.html } } While constructing the above decay tree I have used the branch which } } has the highest branch ratio (above 99% in each case). } } } } I do not understand why you are trying to keep things "under control"? } } } } By the way, I'm a Nuclear Engineer. } } } } One does not even need to be nuclear engineer to understand this. Even } } in high school science classes people learn about radioactive decay } } series e.g. A decays into B and B decays into C... } } } } Best, } } Ayten. } } } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } ===========================
I'm definitely not an expert in this field but from a bit of reading it seems that it is anything but a clear and simple story and measurement is clearly the way to go. In my poking around and reading I did run into a mention of U-238 giving off gamma rays as a result of alpha emission. Maybe this explains the gamma ray emission that Paul Beauregard reported. For what it's worth.......
Alpha particles (named after and denoted by the first letter in the Greek alphabet, α) consist of two protons and two neutrons bound together into a particle identical to a helium nucleus; hence, it can be written as He2+ or 42He2+. They are a highly ionizing form of particle radiation, and have low penetration.
Alpha particles are emitted by radioactive nuclei such as uranium, thorium, actinium, or radium in a process known as alpha decay. This sometimes leaves the nucleus in an excited state, with the emission of a gamma ray removing the excess energy.
dkloos-at-parallaxray.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Based on Dale's reported half life for U238, the specific activity for U238 } would be 1.2x10exp4 DPS, or about 0.3 uCi/g. This is very small activity } and they're alpha particles, which can be shielded best by plastic to avoid } Bremstrahlung and secondary x-ray production. The daughter products will } accumulate and decay according to their schemes and half-lives, which makes } it more difficult to calculate. (See "Nuclear and Radiochemistry", by } Friedlander, Kennedy, Miller, for a treatise on the differential equations } to calculate activity from multiple decay pathways.) } } The best is to just measure it with a survey meter and see what the external } total dose rate in mr/hr is. Don't be afraid if your meter 'pegs' on the } lowest (most sensitive setting). A sample with a field over several mr/hr } over background should be handled according to practices used for handling } radioactive materials. } } Hope that helps a bit. } } Don Kloos } VP Sales, Marketing, Business Development } Parallax Research } } (Ex-Radiochemist) } } } Sales & Marketing } 16478 Beach Blvd. #330 } Westminster, California, 92683-7860 USA } } TOLL FREE 1 866 581-XRAY (9729) } Telephone 1 714 897-9779 } Fax 1 714 897-1421 } Email: dkloos-at-parallaxray.com } SKYPE: don.kloos } Website: http://www.parallaxray.com } } } -----Original Message----- } X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] } Sent: Saturday, January 10, 2009 8:59 AM } To: dkloos-at-parallaxray.com } Subject: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm sorry about sounding a bit negative in my previous post. I did not } mean to school anybody. } } I just wanted to clarify that the radioactive decay process of U-238 } continues via further decay of daughter nuclei with different modes of } decay. And people can do their own calculations, as well. } } Regards, } Ayten. } } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } =========================== } } On Fri, Jan 9, 2009 at 4:44 PM, {dac-at-research.umass.edu} wrote: } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } Dear Ayten, } } } } Your comments are informative but, to my personal taste, definitely a } } bit negative in tone for this list. Your "schooling" of Alex isn't } } really necessary and I think we could get your information in a more } } positive and collegial way. } } } } Regarding the decay tree, I note from the provided link information that } } the half-life of U-238 is { {4.468E+9 years} } . It has been a while since } } my high school chemistry but I'm wondering how much Th-234 and } } associated beta emission danger we are really dealing with here; seems } } like there must be a very small amount of Th-234 produced with such a } } long half-life of the original U-238. Maybe you could comment on the } } danger of this. } } } } Thanks, } } } } Dale } } } } } } celikaktas-at-gmail.com wrote: } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } Dear Alex, } } } } } } Did you have a chance to check the information from } } } } } } http://atom.kaeri.re.kr/ton/nuc7.html } } } } } } Let me construct the decay tree. I'm sure everybody in this list can } } } do it but, you keep insisting that "uranyl compounds are alpha } } } emitters only" so, I will take the time to do the job and post in to } } } the list. } } } } } } Let's start with U-238 which is the starting element in your compound. } } } } } } 1) U-238 decays into Th-234 by Alpha decay } } } } } } 2) Th-234 decays into Pa-234 by Beta decay } } } } } } 3) Pa-234 decays into U-234 by Beta decay } } } } } } 4) U-234 decays into Th-230 by Alpha decay } } } } } } 5) Th-230 decays into Ra-226 by Alpha decay } } } } } } 6) Ra-226 decays into Rn-222 by Alpha decay } } } } } } 7) Rn-222 decays into Po-218 by Alpha decay } } } } } } 8) Po-218 decays into Pb-214 by Alpha decay } } } } } } 9) Pb-214 decays into Bi-214 by Beta decay } } } } } } 10) Bi-214 decays into Po-214 by Beta decay } } } } } } 11) Po-214 decays into Pb-210 by Alpha decay } } } } } } 12) Pb-210 decays into Bi-210 by Beta decay } } } } } } 13) Bi-210 decays into Po-210 by Beta decay } } } } } } 14) Po-210 decays into Pb-206 by Alpha decay } } } } } } Pb-206 is STABLE so, it is the last element to be produced as a result } } } of U-238 radioactive decay. } } } } } } I have constructed the above decay tree using the information from } } } http://atom.kaeri.re.kr/ton/nuc7.html } } } While constructing the above decay tree I have used the branch which } } } has the highest branch ratio (above 99% in each case). } } } } } } I do not understand why you are trying to keep things "under control"? } } } } } } By the way, I'm a Nuclear Engineer. } } } } } } One does not even need to be nuclear engineer to understand this. Even } } } in high school science classes people learn about radioactive decay } } } series e.g. A decays into B and B decays into C... } } } } } } Best, } } } Ayten. } } } } } -- } } =========================== } } Ayten Celik-Aktas, PhD } } Ankara University } } Electron Microscopy Laboratory } } Ankara, Turkey } } =========================== } } ==============================Original Headers============================== } 7, 34 -- From celikaktas-at-gmail.com Sat Jan 10 10:50:09 2009 } 7, 34 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com } [72.14.220.157]) } 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n0AGo8B5031858 } 7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 10:50:09 } -0600 } 7, 34 -- Received: by fg-out-1718.google.com with SMTP id 13so3751505fge.4 } 7, 34 -- for {Microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 08:50:08 } -0800 (PST) } 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 34 -- d=gmail.com; s=gamma; } 7, 34 -- } h=domainkey-signature:mime-version:received:in-reply-to:references } 7, 34 -- :date:message-id:subject:from:to:content-type } 7, 34 -- :content-transfer-encoding; } 7, 34 -- bh=b7SWPFZhMaWulbg1hGRPoUW6eYkvHtar8baNSsG43Z4=; } 7, 34 -- } b=m6xReT0PDvt+APSQ6xOAxowj5D8DRGNg/bb+JG79oNc2HYhEhuTh9MtHO4oxtZFgiJ } 7, 34 -- } 6gUlLRLqq6iO9QomoUY2ApzZvVy5ibE25mjMdYIw36ykLMNyGPxvd0geHyrIE/HcbJTw } 7, 34 -- sy3ySqK30W1KHQSrpz3P2BccaH10kqzXJON3w= } 7, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 7, 34 -- d=gmail.com; s=gamma; } 7, 34 -- } h=mime-version:in-reply-to:references:date:message-id:subject:from:to } 7, 34 -- :content-type:content-transfer-encoding; } 7, 34 -- } b=x6wLqhBvlIB7ko+B5XBu53zczgEI647iuiv9yDkFq+Xm3ov7YKVjwqQ11peUA1lb9N } 7, 34 -- } k31yM6C+OV/4dQcHT4SGtgbvtbt2wRrDGp4CJpXUZ+sNM91L9SEMCvmkc7tIQJutoUYR } 7, 34 -- BjkHZIK2Q5wsp3wzSv9YsQXZX0KASiwGb5WwE= } 7, 34 -- MIME-Version: 1.0 } 7, 34 -- Received: by 10.103.217.5 with SMTP id } u5mr9668029muq.42.1231606207534; Sat, } 7, 34 -- 10 Jan 2009 08:50:07 -0800 (PST) } 7, 34 -- In-Reply-To: {200901091444.n09Ei6HG011125-at-ns.microscopy.com} } 7, 34 -- References: {200901091444.n09Ei6HG011125-at-ns.microscopy.com} } 7, 34 -- Date: Sat, 10 Jan 2009 18:50:07 +0200 } 7, 34 -- Message-ID: } {1075c5c10901100850u20dcb242nd050b7702a79e92d-at-mail.gmail.com} } 7, 34 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha } emitters } 7, 34 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} } 7, 34 -- To: microscopy {Microscopy-at-microscopy.com} } 7, 34 -- Content-Type: text/plain; charset=UTF-8 } 7, 34 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 18, 30 -- From dkloos-at-parallaxray.com Sat Jan 10 14:35:05 2009 } 18, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) } 18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AKZ5fk021450 } 18, 30 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 14:35:05 -0600 } 18, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) } 18, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) } 18, 30 -- (envelope-from {dkloos-at-parallaxray.com} ) } 18, 30 -- id 1LLkY3-0009W7-On; Sat, 10 Jan 2009 13:35:11 -0700 } 18, 30 -- Reply-To: {dkloos-at-parallaxray.com} } 18, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com} } 18, 30 -- To: {celikaktas-at-gmail.com} } 18, 30 -- Cc: {microscopy-at-microscopy.com} } 18, 30 -- References: {200901101659.n0AGx43H012719-at-ns.microscopy.com} } 18, 30 -- Subject: RE: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters } 18, 30 -- Date: Sat, 10 Jan 2009 12:34:58 -0800 } 18, 30 -- Organization: Parallax Research } 18, 30 -- Message-ID: {E3F6FCD7F3614856949442747BC989B8-at-donl} } 18, 30 -- MIME-Version: 1.0 } 18, 30 -- Content-Type: text/plain; } 18, 30 -- charset="us-ascii" } 18, 30 -- Content-Transfer-Encoding: 7bit } 18, 30 -- X-Mailer: Microsoft Office Outlook 11 } 18, 30 -- Thread-Index: AclzRMI1owznMu3ZRCWFEukvLFTfPAAHKnng } 18, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 } 18, 30 -- In-Reply-To: {200901101659.n0AGx43H012719-at-ns.microscopy.com} } 18, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report } 18, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com } 18, 30 -- X-AntiAbuse: Original Domain - microscopy.com } 18, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] } 18, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 20 -- From dac-at-research.umass.edu Sat Jan 10 17:06:10 2009 9, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0AN69aV006061 9, 20 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 17:06:09 -0600 9, 20 -- Received: from [192.168.1.100] (static.unknown.charter.com [96.39.6.64] (may be forged)) 9, 20 -- (authenticated bits=0) 9, 20 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0AN68eV026533 9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 20 -- for {microscopy-at-microscopy.com} ; Sat, 10 Jan 2009 18:06:08 -0500 9, 20 -- Message-ID: {49692A12.7080904-at-research.umass.edu} 9, 20 -- Date: Sat, 10 Jan 2009 18:06:58 -0500 9, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 9, 20 -- MIME-Version: 1.0 9, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters 9, 20 -- References: {200901102040.n0AKekds029574-at-ns.microscopy.com} 9, 20 -- In-Reply-To: {200901102040.n0AKekds029574-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=UTF-8; format=flowed 9, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both przybylowicz-at-tlabs.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: przybylowicz-at-tlabs.ac.za Name: Wojciech Przybylowicz
Organization: iThemba LABS, South Africa
Title-Subject: [Filtered] Post-doctoral fellow (NMP) - two years contract with extension possibility
Question: Post-doctoral fellow (NMP) - two years contract with extension possibility
Position: Nuclear microprobe analysis of thin frozen-hydrated biological specimens
Applications are invited for this position in our Materials Research Group (MRG) of iThemba LABS, situated ca. 30 km from Cape Town, South Africa.
Responsibilities will include: - Adapting the cryo-stage coupled to the MRG nuclear microprobe for measurements of thin specimens in frozen-hydrated state - Active involvement in research projects related to biological applications of ion beam techniques and generating new applications.
Minimum requirements:
- PhD in Biology/Chemistry/Physics with strong emphasis on cryo-preparation of biological specimens and low temperature electron microscopy - Experience in operating SEM/TEM and knowledge of EDS technique as well as cryo-ultramicrotomy - Experience in operating a nuclear microprobe and familiarity with PIXE as an advantage - Relevant conference presentations and publications as well as international exposure - Knowledge of statistical methods - Computer literacy (Word, Excel, Corel, etc.)
We offer a competitive remuneration package, which includes normal company benefits.
Forward your detailed CV, accompanied by a covering letter and supporting documents, to the Human Resource Department; iThemba LABS, P.O. Box 722, Somerset West 7129, or fax (+27-21-8433756), or via e-mail to: mirencia-at-tlabs.ac.za with copy to przybylowicz-at-tlabs.ac.za For some information of the laboratory, visit our website: www.tlabs.ac.za
I suggest that you contact me for any details of this position. E-mail: przybylowicz-at-tlabs.ac.za You may want to read the following publication on earlier work done by our team in this direction:
Grzegorz Tylko, Jolanta Mesjasz-Przybyłowicz and Wojciech J. Przybyłowicz. X-ray microanalysis of biological material in the frozen-hydrated state by PIXE. Microscopy Research and Technique (2007) 70: 55-68.
I have a TEM project, where the researcher wants to observe their monolayer cell culture and the calcium crystals produced by those cells. The cells produce extracellular calcium crystals which dissolve readily in water. The usual fixation, post-fix, dehydration steps won't work . This situation is unlike anything I have dealt with before, including all my years of working with marine invertebrates. I am speculating that cryo methods may be the only answer. As always thanks in advance for any help possible.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CGw3WY025453 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:03 -0600 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C8AFDA0076 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:02 -0600 (CST) 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:00 -0600 (CST) 5, 20 -- Subject: TEM:cell culture with extracellular calcium 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- Message-ID: {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09 5, 20 -- AM 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
The obvious question would seem to be "How do the extracellular calcium crystals survive in aqueous tissue culture medium?" Are you sure they are dissolving or simply being washed away.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Monday, January 12, 2009 10:59 AM To: Phillips, Thomas E.
Listers,
I have a TEM project, where the researcher wants to observe their monolayer cell culture and the calcium crystals produced by those cells. The cells produce extracellular calcium crystals which dissolve readily in water. The usual fixation, post-fix, dehydration steps won't work . This situation is unlike anything I have dealt with before, including all my years of working with marine invertebrates. I am speculating that cryo methods may be the only answer. As always thanks in advance for any help possible.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CGw3WY025453 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:03 -0600 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C8AFDA0076 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:02 -0600 (CST) 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:00 -0600 (CST) 5, 20 -- Subject: TEM:cell culture with extracellular calcium 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- Message-ID: {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09 5, 20 -- AM 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 14, 29 -- From PhillipsT-at-missouri.edu Mon Jan 12 11:06:11 2009 14, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CH6BPt006584 14, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 11:06:11 -0600 14, 29 -- X-IronPort-Anti-Spam-Filtered: true 14, 29 -- X-IronPort-Anti-Spam-Result: ApoEAM8Ga0nRauUp/2dsb2JhbADITQ0BCYUihUsBhD+BLw 14, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 14, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 12 Jan 2009 11:06:11 -0600 14, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 29 -- Mon, 12 Jan 2009 11:06:11 -0600 14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 29 -- Content-class: urn:content-classes:message 14, 29 -- MIME-Version: 1.0 14, 29 -- Content-Type: text/plain; 14, 29 -- charset="us-ascii" 14, 29 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium 14, 29 -- Date: Mon, 12 Jan 2009 11:06:10 -0600 14, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05BBD766-at-UM-XMAIL06.um.umsystem.edu} 14, 29 -- In-Reply-To: {200901121659.n0CGx0wY026584-at-ns.microscopy.com} 14, 29 -- X-MS-Has-Attach: 14, 29 -- X-MS-TNEF-Correlator: 14, 29 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium 14, 29 -- thread-index: Acl01xIfWxQoeHbdTM+DDiefgUiB8QAANJ1w 14, 29 -- References: {200901121659.n0CGx0wY026584-at-ns.microscopy.com} 14, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 29 -- To: {Microscopy-at-microscopy.com} 14, 29 -- X-OriginalArrivalTime: 12 Jan 2009 17:06:11.0130 (UTC) FILETIME=[117359A0:01C974D8] 14, 29 -- Content-Transfer-Encoding: 8bit 14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CH6BPt006584 ==============================End of - Headers==============================
On Jan 9, 2009, at 4:07 PM, beaurega-at-westol.com wrote:
} Someone strongly pointed out that U is an alpha emitter, not a gamma } emitter, and I was not reading gamma radiation (but I was). You } pointed } out that it was the decay impurities that were the gamma emitters } and that } was what I was reading on my counter. I agree that U-238 and DU are } still } radioactive.
} Here's my favorite. "depleted uranium is 40% less radioactive than } natural } uranium." That means 60% of the radioactivity is still there. I am } not } sure that includes gamma but probably.
Dear Paul, Since the activities of the U isotopes are inversely proportional to their half-lives, and since the half lives of U235 and U234 are about 7 and 20,000 times shorter respectively than U238's, the amount of radiation from U234 is about equal to that from U238, and that from U235 is about 5% of that from the others, so your quote that DU has only 60% the activity of natural U checks out. I pointed out that ~1/4 of the U238 decays are to an excited state of Th234, which is ~50 keV above the ground state, so pure U238 will produce some low-energy gammas, as will many of the daughters. The bottom line is that direct measurements performed correctly don't lie, so they are the best way to settle this issue once and for all. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 7, 22 -- From tivol-at-caltech.edu Mon Jan 12 11:20:46 2009 7, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHKj7C020231 7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 11:20:46 -0600 7, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 7, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 95B5766E1E4F 7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 09:20:45 -0800 (PST) 7, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 7, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 7, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 7E59866E2F4D 7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 09:20:44 -0800 (PST) 7, 22 -- Message-Id: {C463CBB1-CCFF-43DE-A64B-E5D1522D3FA8-at-caltech.edu} 7, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 7, 22 -- To: microscopy-at-microscopy.com 7, 22 -- In-Reply-To: {200901100007.n0A07fpE026798-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 7, 22 -- Subject: Re: [Microscopy] Re: viaWWW: uranyl compounds are alpha emitters 7, 22 -- Date: Mon, 12 Jan 2009 09:20:42 -0800 7, 22 -- References: {200901100007.n0A07fpE026798-at-ns.microscopy.com} 7, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I have been searching around for some time and I have not been able to find a supplier with any Nanoplast (melamine) resin. Does anyone know what company actually produced Nanoplast, if they are still operating, or if there is a supplier who still has some?
Thanks,
Kasim Sader
---------------------- Dr Kasim Sader SuperSTEM Daresbury Laboratory Warrington WA4 4AD
==============================Original Headers============================== 10, 30 -- From K.Sader-at-leeds.ac.uk Mon Jan 12 11:56:48 2009 10, 30 -- Received: from mhost03c.leeds.ac.uk (mhost03c.leeds.ac.uk [129.11.76.167]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHulM7002354 10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 11:56:48 -0600 10, 30 -- Received: from APOLLO1.ds.leeds.ac.uk (apollo1.leeds.ac.uk [129.11.5.4]) 10, 30 -- by mhost03c.leeds.ac.uk (8.14.3/8.14.3) with ESMTP id n0CHukS2019389 10, 30 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 17:56:46 GMT 10, 30 -- Received: from dexbr1.ds.leeds.ac.uk (129.11.76.19) by APOLLO1.ds.leeds.ac.uk 10, 30 -- (129.11.5.4) with Microsoft SMTP Server id 8.1.311.2; Mon, 12 Jan 2009 10, 30 -- 17:56:45 +0000 10, 30 -- Received: from HERMES4.ds.leeds.ac.uk ([129.11.5.130]) by 10, 30 -- dexbr1.ds.leeds.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); Mon, 12 Jan 10, 30 -- 2009 17:56:45 +0000 10, 30 -- Received: from 129.11.40.48 ([129.11.40.48]) by HERMES4.ds.leeds.ac.uk 10, 30 -- ([129.11.5.131]) via Exchange Front-End Server outlook.leeds.ac.uk 10, 30 -- ([129.11.5.3]) with Microsoft Exchange Server HTTP-DAV ; Mon, 12 Jan 2009 10, 30 -- 17:56:45 +0000 10, 30 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 30 -- Date: Mon, 12 Jan 2009 17:56:44 +0000 10, 30 -- Subject: Nanoplast resin 10, 30 -- From: Kasim Sader {k.sader-at-leeds.ac.uk} 10, 30 -- To: {Microscopy-at-Microscopy.Com} 10, 30 -- Message-ID: {C59134DC.619C%k.sader-at-leeds.ac.uk} 10, 30 -- Thread-Topic: Nanoplast resin 10, 30 -- Thread-Index: Acl03yEuX9NSRODSEd218gAZ4zduOA== 10, 30 -- MIME-Version: 1.0 10, 30 -- Content-Type: text/plain; charset="US-ASCII" 10, 30 -- Content-Transfer-Encoding: 7bit 10, 30 -- X-OriginalArrivalTime: 12 Jan 2009 17:56:45.0245 (UTC) FILETIME=[21EC8ED0:01C974DF] ==============================End of - Headers==============================
Ca products will either dissolve or wash away, but will disappear from the external and internal areas of the cells during aqueous sample preparation.You really can't avoid this happening. The only way to minimize the amount of dissolving (but not necessarily washing away if external) of the Ca product is using HPF and FS.
I say this based on personal experience with internal calcium carbonate inclusions in cells.
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: {tbargar-at-unmc.edu} } Reply-To: {tbargar-at-unmc.edu} } Date: Mon, 12 Jan 2009 11:00:03 -0600 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] TEM:cell culture with extracellular calcium } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Listers, } } I have a TEM project, where the researcher wants to observe their monolayer } cell culture and the calcium crystals produced by those cells. The cells } produce extracellular calcium crystals which dissolve readily in water. } The usual fixation, post-fix, dehydration steps won't work . This situation } is unlike anything I have dealt with before, including all my years of } working with marine invertebrates. I am speculating that cryo methods may } be the only answer. As always thanks in advance for any help possible. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original Headers============================== } 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 } 5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) } 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n0CGw3WY025453 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:03 -0600 } 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) } 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C8AFDA0076 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:02 -0600 } (CST) } 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu } [137.197.103.35]) } 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:00 -0600 } (CST) } 5, 20 -- Subject: TEM:cell culture with extracellular calcium } 5, 20 -- To: Microscopy-at-microscopy.com } 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 } 5, 20 -- Message-ID: } {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu} } 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} } 5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600 } 5, 20 -- X-MIMETrack: Serialize by Router on } UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09 } 5, 20 -- AM } 5, 20 -- MIME-Version: 1.0 } 5, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 30 -- From dsherman-at-purdue.edu Mon Jan 12 11:57:57 2009 8, 30 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHvvYQ004818 8, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 11:57:57 -0600 8, 30 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 8, 30 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n0CHvvA5020838 8, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 12:57:57 -0500 8, 30 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 8, 30 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n0CHvvne000488 8, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 12:57:57 -0500 8, 30 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 30 -- Mon, 12 Jan 2009 12:57:56 -0500 8, 30 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ; 8, 30 -- Mon, 12 Jan 2009 17:57:08 +0000 8, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119 8, 30 -- Date: Mon, 12 Jan 2009 12:57:07 -0500 8, 30 -- Subject: Re: [Microscopy] TEM:cell culture with extracellular calcium 8, 30 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 30 -- To: {tbargar-at-unmc.edu} , "message to: MSA list" {microscopy-at-microscopy.com} 8, 30 -- Message-ID: {C590EEA3.38C43%dsherman-at-exchange.purdue.edu} 8, 30 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium 8, 30 -- Thread-Index: Acl03y7ktOL8Rky8Tk6hZdzF0JB74Q== 8, 30 -- In-Reply-To: {200901121700.n0CH03iD028637-at-ns.microscopy.com} 8, 30 -- Mime-version: 1.0 8, 30 -- Content-type: text/plain; 8, 30 -- charset="US-ASCII" 8, 30 -- Content-transfer-encoding: 7bit 8, 30 -- X-OriginalArrivalTime: 12 Jan 2009 17:57:56.0527 (UTC) FILETIME=[4C6953F0:01C974DF] 8, 30 -- X-PMX-Version: 5.4.0.320885 8, 30 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
It's a long shot, but there are non-aqueous fixation techniques that were originally developed to preserve biofilms that are usually removed during conventional processing methods. These use a perfluorocarbon solvent, such as the 3M company's FC-72, with osmium tetroxide to do the fixation. If the calcium crystals will survive ethanol dehydration, it may work. If not, it may be possible to experiment with ways of getting the cells into resin directly from the solvent. Depends on how much fuss you want to go through on a long-shot protocol!
One reference is: "Heterogeneity of the Composition and Thickness of Tracheal Mucus in Rats", 1997, David Sims and Margaret Horne, Am J Phys--Lung Cell & Mol Phsiol 17:L1036-L1041.
I can come up with more if you want to pursue this. 3M used to sell this solvent in small quantities, although it's normally sold in drums for electrical transformers, etc. Don't know if they still have the smaller units.
For what it's worth...
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Monday, January 12, 2009 10:59 AM To: Tindall, Randy D.
Listers,
I have a TEM project, where the researcher wants to observe their monolayer cell culture and the calcium crystals produced by those cells. The cells produce extracellular calcium crystals which dissolve readily in water. The usual fixation, post-fix, dehydration steps won't work . This situation is unlike anything I have dealt with before, including all my years of working with marine invertebrates. I am speculating that cryo methods may be the only answer. As always thanks in advance for any help possible.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CGw3WY025453 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:03 -0600 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id C8AFDA0076 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:02 -0600 (CST) 5, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id A012539804F 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 10:58:00 -0600 (CST) 5, 20 -- Subject: TEM:cell culture with extracellular calcium 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- Message-ID: {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu} 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/12/2009 10:59:09 5, 20 -- AM 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
==============================Original Headers============================== 24, 30 -- From TindallR-at-missouri.edu Mon Jan 12 12:52:48 2009 24, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 24, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CIqmZF030867 24, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 12:52:48 -0600 24, 30 -- X-IronPort-Anti-Spam-Filtered: true 24, 30 -- X-IronPort-Anti-Spam-Result: ApoEANMga0nRauUo/2dsb2JhbADIdg0BCYU9hXCEQIEvhDI 24, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 24, 30 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 12 Jan 2009 12:52:45 -0600 24, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 24, 30 -- Mon, 12 Jan 2009 12:52:45 -0600 24, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 30 -- Content-class: urn:content-classes:message 24, 30 -- MIME-Version: 1.0 24, 30 -- Content-Type: text/plain; 24, 30 -- charset="us-ascii" 24, 30 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium 24, 30 -- Date: Mon, 12 Jan 2009 12:52:45 -0600 24, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7CE0-at-UM-XMAIL08.um.umsystem.edu} 24, 30 -- In-Reply-To: {200901121659.n0CGxESU027070-at-ns.microscopy.com} 24, 30 -- X-MS-Has-Attach: 24, 30 -- X-MS-TNEF-Correlator: 24, 30 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium 24, 30 -- thread-index: Acl01xlVhTneargmT7eRSno8qDklvwADa9Ug 24, 30 -- References: {200901121659.n0CGxESU027070-at-ns.microscopy.com} 24, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 24, 30 -- To: {tbargar-at-unmc.edu} 24, 30 -- Cc: {microscopy-at-microscopy.com} 24, 30 -- X-OriginalArrivalTime: 12 Jan 2009 18:52:45.0431 (UTC) FILETIME=[F4C04070:01C974E6] 24, 30 -- Content-Transfer-Encoding: 8bit 24, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CIqmZF030867 ==============================End of - Headers==============================
Sounds like rapid freezing followed by freeze substitution is probably the way to go (as Debby Sherman suggested).
If you do not have access to such equipment, another possibility would be to grow the cells directly on a TEM grid (with a Formvar film, of course). Cu grids may be toxic to some cells but Ni should be OK. We've grown cells on grids in the past and it works well once you work out the concentration of cells (so as not to obliterate the view).
In our situation, we rinsed to remove culture fluids but you could plunge freeze the grid and freeze dry it. We made a freeze dryer (of sorts) by having a copper block machined to accommodate specimens. It had a lid (to prevent condensation). We loaded the specimens into the LN2-chilled block and then put it in a vacuum evaporator. In your case, 8 hr should dry the grid.
JB
} I have a TEM project, where the researcher wants to observe their monolayer } cell culture and the calcium crystals produced by those cells. The cells } produce extracellular calcium crystals which dissolve readily in water. } The usual fixation, post-fix, dehydration steps won't work . This situation } is unlike anything I have dealt with before, including all my years of } working with marine invertebrates. I am speculating that cryo methods may } be the only answer. As always thanks in advance for any help possible. } } Tom Bargar } University of Nebraska Medical Center }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Tom - Just FYI: I used the non-aqueous method mentioned by Randy several years ago, and it worked well for preserving the mucous layer on murine intestinal tissue. The reference he suggested is a good start. Also, if you call 3M, they may send you a sample bottle of Fluorinert FC-72, which may be enough for your needs (and was enough for me to do a trial run to ensure that the fixation worked).
Jessica
Jessica Cervantes Bend Research, Inc Bend, OR
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Tom,
It's a long shot, but there are non-aqueous fixation techniques that were originally developed to preserve biofilms that are usually removed during conventional processing methods. These use a perfluorocarbon solvent, such as the 3M company's FC-72, with osmium tetroxide to do the fixation. If the calcium crystals will survive ethanol dehydration, it may work. If not, it may be possible to experiment with ways of getting the cells into resin directly from the solvent. Depends on how much fuss you want to go through on a long-shot protocol!
One reference is: "Heterogeneity of the Composition and Thickness of Tracheal Mucus in Rats", 1997, David Sims and Margaret Horne, Am J Phys--Lung Cell & Mol Phsiol 17:L1036-L1041.
I can come up with more if you want to pursue this. 3M used to sell this solvent in small quantities, although it's normally sold in drums for electrical transformers, etc. Don't know if they still have the smaller units.
For what it's worth...
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Monday, January 12, 2009 10:59 AM To: Tindall, Randy D.
Listers,
I have a TEM project, where the researcher wants to observe their monolayer cell culture and the calcium crystals produced by those cells. The cells produce extracellular calcium crystals which dissolve readily in water. The usual fixation, post-fix, dehydration steps won't work . This situation is unlike anything I have dealt with before, including all my years of working with marine invertebrates. I am speculating that cryo methods may be the only answer. As always thanks in advance for any help possible.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 32, 22 -- From cervantes-at-bendres.com Mon Jan 12 13:35:22 2009 32, 22 -- Received: from smtp.bendres.com (smtp.bendres.com [67.59.84.113]) 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CJZLnf026911 32, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:35:22 -0600 32, 22 -- Content-class: urn:content-classes:message 32, 22 -- MIME-Version: 1.0 32, 22 -- Content-Type: text/plain; 32, 22 -- charset="us-ascii" 32, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 32, 22 -- Subject: RE: [Microscopy] RE: TEM:cell culture with extracellular calcium - non-aqueous fixation 32, 22 -- Date: Mon, 12 Jan 2009 11:35:19 -0800 32, 22 -- Message-ID: {82C755170EE5C44BA26E35A7F6B3864A040C29-at-dixie.bri.local} 32, 22 -- In-Reply-To: {200901121856.n0CIuwoH006559-at-ns.microscopy.com} 32, 22 -- X-MS-Has-Attach: 32, 22 -- X-MS-TNEF-Correlator: 32, 22 -- Thread-Topic: [Microscopy] RE: TEM:cell culture with extracellular calcium - non-aqueous fixation 32, 22 -- Thread-Index: Acl054ygcHZ11egoRkyDVWjg+NhE+wAA3RtA 32, 22 -- References: {200901121856.n0CIuwoH006559-at-ns.microscopy.com} 32, 22 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 32, 22 -- To: {Microscopy-at-microscopy.com} 32, 22 -- Content-Transfer-Encoding: 8bit 32, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CJZLnf026911 ==============================End of - Headers==============================
There was an old paper about anhydrous specimen preparation for TEM: http://www.ncbi.nlm.nih.gov/pubmed/66323
Method used ethylene glycol, cellosolve and propylene oxide. Embedded in Epon specimens were cut with knife filled with ethylene glycol. A while ago I used this method and got some results.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] } Sent: Monday, January 12, 2009 10:59 AM } To: Dusevich, Vladimir } Subject: [Microscopy] TEM:cell culture with extracellular calcium } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } } Listers, } } I have a TEM project, where the researcher wants to observe } their monolayer cell culture and the calcium crystals } produced by those cells. The cells produce extracellular } calcium crystals which dissolve readily in water. } The usual fixation, post-fix, dehydration steps won't work . } This situation is unlike anything I have dealt with before, } including all my years of working with marine invertebrates. } I am speculating that cryo methods may be the only answer. } As always thanks in advance for any help possible. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original } Headers============================== } 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20 } -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu } [192.198.54.127]) } 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n0CGw3WY025453 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 10:58:03 -0600 } 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) } 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP } id C8AFDA0076 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 10:58:02 -0600 (CST) } 5, 20 -- Received: from unmcnotes01.unmc.edu } (host-137-197-103-35.unmc.edu [137.197.103.35]) } 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP } id A012539804F } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 10:58:00 -0600 (CST) } 5, 20 -- Subject: TEM:cell culture with extracellular calcium } 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer: } Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- } Message-ID: } {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu} } 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: } Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack: } Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at } 01/12/2009 10:59:09 5, 20 -- AM 5, 20 -- MIME-Version: 1.0 } 5, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 26 -- From DusevichV-at-umkc.edu Mon Jan 12 14:15:43 2009 8, 26 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CKFhDZ009316 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 14:15:43 -0600 8, 26 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Mon, 12 Jan 2009 14:15:42 -0600 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium 8, 26 -- Date: Mon, 12 Jan 2009 14:15:42 -0600 8, 26 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AC-at-KC-MSX1.kc.umkc.edu} 8, 26 -- In-Reply-To: {200901121658.n0CGwj2i026350-at-ns.microscopy.com} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium 8, 26 -- thread-index: Acl01wj0f9lD3FrtTi+bEOThxFeWogAGlcKA 8, 26 -- References: {200901121658.n0CGwj2i026350-at-ns.microscopy.com} 8, 26 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 26 -- To: {tbargar-at-unmc.edu} , {microscopy-at-msa.microscopy.com} , 8, 26 -- {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 12 Jan 2009 20:15:42.0772 (UTC) FILETIME=[8B7A3740:01C974F2] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CKFhDZ009316 ==============================End of - Headers==============================
My ancient files have yielded a manufacturer for NANOPLAST FB 101. Google Rolf Bachhuber and a 2002 paper comes up with manufacturer in Germany. I'd type it out but my typing isn't to be trusted.
A publication from 'Agar Scientific' in May 1990 has references about melamine resins. That company was in the U.K. at the time.
I have an old Nanoplast kit from Polyscience in the U.S. I know they don't do any manufacturing and I have no idea if they still carry it.
Good luck,
Grete Adamson EM Med Path U.C. Davis
-----Original Message----- X-from: k.sader-at-leeds.ac.uk [mailto:k.sader-at-leeds.ac.uk] Sent: Monday, January 12, 2009 10:05 AM To: Adamson, Grete
Hi,
I have been searching around for some time and I have not been able to find a supplier with any Nanoplast (melamine) resin. Does anyone know what company actually produced Nanoplast, if they are still operating, or if there is a supplier who still has some?
Thanks,
Kasim Sader
---------------------- Dr Kasim Sader SuperSTEM Daresbury Laboratory Warrington WA4 4AD
==============================Original Headers============================== 10, 30 -- From K.Sader-at-leeds.ac.uk Mon Jan 12 11:56:48 2009 10, 30 -- Received: from mhost03c.leeds.ac.uk (mhost03c.leeds.ac.uk [129.11.76.167]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CHulM7002354 10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 11:56:48 -0600 10, 30 -- Received: from APOLLO1.ds.leeds.ac.uk (apollo1.leeds.ac.uk [129.11.5.4]) 10, 30 -- by mhost03c.leeds.ac.uk (8.14.3/8.14.3) with ESMTP id n0CHukS2019389 10, 30 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 10, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 Jan 2009 17:56:46 GMT 10, 30 -- Received: from dexbr1.ds.leeds.ac.uk (129.11.76.19) by APOLLO1.ds.leeds.ac.uk 10, 30 -- (129.11.5.4) with Microsoft SMTP Server id 8.1.311.2; Mon, 12 Jan 2009 10, 30 -- 17:56:45 +0000 10, 30 -- Received: from HERMES4.ds.leeds.ac.uk ([129.11.5.130]) by 10, 30 -- dexbr1.ds.leeds.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); Mon, 12 Jan 10, 30 -- 2009 17:56:45 +0000 10, 30 -- Received: from 129.11.40.48 ([129.11.40.48]) by HERMES4.ds.leeds.ac.uk 10, 30 -- ([129.11.5.131]) via Exchange Front-End Server outlook.leeds.ac.uk 10, 30 -- ([129.11.5.3]) with Microsoft Exchange Server HTTP-DAV ; Mon, 12 Jan 2009 10, 30 -- 17:56:45 +0000 10, 30 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 30 -- Date: Mon, 12 Jan 2009 17:56:44 +0000 10, 30 -- Subject: Nanoplast resin 10, 30 -- From: Kasim Sader {k.sader-at-leeds.ac.uk} 10, 30 -- To: {Microscopy-at-Microscopy.Com} 10, 30 -- Message-ID: {C59134DC.619C%k.sader-at-leeds.ac.uk} 10, 30 -- Thread-Topic: Nanoplast resin 10, 30 -- Thread-Index: Acl03yEuX9NSRODSEd218gAZ4zduOA== 10, 30 -- MIME-Version: 1.0 10, 30 -- Content-Type: text/plain; charset="US-ASCII" 10, 30 -- Content-Transfer-Encoding: 7bit 10, 30 -- X-OriginalArrivalTime: 12 Jan 2009 17:56:45.0245 (UTC) FILETIME=[21EC8ED0:01C974DF] ==============================End of - Headers==============================
==============================Original Headers============================== 23, 25 -- From gnadamson-at-ucdavis.edu Mon Jan 12 15:26:03 2009 23, 25 -- Received: from Mail.ucdsom.ucdavis.edu (mail.ucdsom.ucdavis.edu [169.237.87.32]) 23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CLQ3pf024857 23, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 15:26:03 -0600 23, 25 -- Received: from Mail.ucdsom.ucdavis.edu ([169.237.87.32]) by 23, 25 -- Mail.ucdsom.ucdavis.edu ([169.237.87.32]) with mapi; Mon, 12 Jan 2009 23, 25 -- 13:26:29 -0800 23, 25 -- From: "Adamson, Grete" {gnadamson-at-ucdavis.edu} 23, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 23, 25 -- CC: "'k.sader-at-leeds.ac.uk'" {k.sader-at-leeds.ac.uk} 23, 25 -- Date: Mon, 12 Jan 2009 13:26:29 -0800 23, 25 -- Subject: RE: [Microscopy] Nanoplast resin 23, 25 -- Thread-Topic: [Microscopy] Nanoplast resin 23, 25 -- Thread-Index: Acl04FtAL+NoPsEYRYC3hsNvOUTuBQAGnZoA 23, 25 -- Message-ID: {FAB730F779268A468497EFF961DBBE11BA914134AD-at-Mail.ucdsom.ucdavis.edu} 23, 25 -- In-Reply-To: {200901121804.n0CI4qU1028997-at-ns.microscopy.com} 23, 25 -- Accept-Language: en-US 23, 25 -- Content-Language: en-US 23, 25 -- X-MS-Has-Attach: 23, 25 -- X-MS-TNEF-Correlator: 23, 25 -- acceptlanguage: en-US 23, 25 -- Content-Type: text/plain; charset="us-ascii" 23, 25 -- MIME-Version: 1.0 23, 25 -- Content-Transfer-Encoding: 8bit 23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CLQ3pf024857 ==============================End of - Headers==============================
This is a message I never thought I would be sending.
We are getting out of the film and paper photo business, and have a lot of 'stuff' to give away or send to the landfill. Anyone with questions about whether digital imaging for EM is for real, let this be your wake-up call.
Most of what we have is old, probably not worth the cost to ship, unless you are desperate, or curious.
We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572. I don't even know what some of this was used for, if it rings your bell, let me know and I can tell you more.
We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4 x5 sizes.
A little 4463 and a ton of SO-163. The SO-163 is old.
Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan.
Most of this junk has been in a freezer. Some is pretty old and past its expiration date, but if you are interested, let me know and we can try to work something out. If I don't hear anything in the next week or so, its outa here.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 14, 42 -- From jkrupp-at-deltacollege.edu Mon Jan 12 15:59:23 2009 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0CLxMKe006917 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 15:59:23 -0600 14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 14, 42 -- by localhost (Email Security Appliance) with SMTP id 7D4C918E05E 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 21:34:33 +0000 (GMT) 14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 14, 42 -- by mailin.deltacollege.edu (Email Security Appliance) with ESMTP id 726D520CAFD 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 21:34:33 +0000 (GMT) 14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 14, 42 -- with ESMTP id 44930245 for Microscopy-at-microscopy.com; Mon, 12 Jan 2009 13:59:19 -0800 14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 14, 42 -- ESMTP id KDDOD300.CM0 for {Microscopy-at-microscopy.com} ; Mon, 12 14, 42 -- Jan 2009 13:43:51 -0800 14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 13E398F74D38 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:59:19 -0800 (PST) 14, 42 -- X-Virus-Scanned: amavisd-new at 14, 42 -- X-Spam-Flag: NO 14, 42 -- X-Spam-Score: -2.324 14, 42 -- X-Spam-Level: 14, 42 -- X-Spam-Status: No, score=-2.324 tagged_above=-10 required=6 tests=[AWL=0.175, 14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] 14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 14, 42 -- with ESMTP id sAJT+MZ3mFOx for {Microscopy-at-microscopy.com} ; 14, 42 -- Mon, 12 Jan 2009 13:59:18 -0800 (PST) 14, 42 -- Received: from [172.20.4.106] (unknown [172.20.4.106]) 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id AEBE88F74D32 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:59:18 -0800 (PST) 14, 42 -- Message-Id: {7C9F6B4C-B8AF-43D7-86A6-495112B71078-at-deltacollege.edu} 14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 14, 42 -- To: Microscopy-at-microscopy.com 14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 42 -- Content-Transfer-Encoding: 7bit 14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 14, 42 -- Subject: Old film etc 14, 42 -- Date: Mon, 12 Jan 2009 13:59:18 -0800 14, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
It turned out that we have a material which emits alpha, beta and gamma rays. I think the original labeling for uranyl compounds which said "alpha emitter" should be changed. Especially, for the reason that there might be microscopists who are using uranyl compounds in their labs but, do not follow the Microscopy List.
Is there anybody in this group who is in a position and willing to contact Nuclear Regulatory Commission on this subject?
Regards, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Mon, Jan 12, 2009 at 8:26 PM, {tivol-at-caltech.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Jan 9, 2009, at 4:07 PM, beaurega-at-westol.com wrote: } } } Someone strongly pointed out that U is an alpha emitter, not a gamma } } emitter, and I was not reading gamma radiation (but I was). You } } pointed } } out that it was the decay impurities that were the gamma emitters } } and that } } was what I was reading on my counter. I agree that U-238 and DU are } } still } } radioactive. } } } Here's my favorite. "depleted uranium is 40% less radioactive than } } natural } } uranium." That means 60% of the radioactivity is still there. I am } } not } } sure that includes gamma but probably. } } } Dear Paul, } Since the activities of the U isotopes are inversely proportional to } their half-lives, and since the half lives of U235 and U234 are about } 7 and 20,000 times shorter respectively than U238's, the amount of } radiation from U234 is about equal to that from U238, and that from } U235 is about 5% of that from the others, so your quote that DU has } only 60% the activity of natural U checks out. I pointed out that } ~1/4 of the U238 decays are to an excited state of Th234, which is ~50 } keV above the ground state, so pure U238 will produce some low-energy } gammas, as will many of the daughters. The bottom line is that direct } measurements performed correctly don't lie, so they are the best way } to settle this issue once and for all. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } }
How did you then dry your grids without leaving crystals?
Stéphane
----- Original Message ---- X-from: "DusevichV-at-umkc.edu" {DusevichV-at-umkc.edu} To: nizets2-at-yahoo.com Sent: Monday, January 12, 2009 9:19:38 PM
There was an old paper about anhydrous specimen preparation for TEM: http://www.ncbi.nlm.nih.gov/pubmed/66323
Method used ethylene glycol, cellosolve and propylene oxide. Embedded in Epon specimens were cut with knife filled with ethylene glycol. A while ago I used this method and got some results.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] } Sent: Monday, January 12, 2009 10:59 AM } To: Dusevich, Vladimir } Subject: [Microscopy] TEM:cell culture with extracellular calcium } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } } Listers, } } I have a TEM project, where the researcher wants to observe } their monolayer cell culture and the calcium crystals } produced by those cells. The cells produce extracellular } calcium crystals which dissolve readily in water. } The usual fixation, post-fix, dehydration steps won't work . } This situation is unlike anything I have dealt with before, } including all my years of working with marine invertebrates. } I am speculating that cryo methods may be the only answer. } As always thanks in advance for any help possible. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original } Headers============================== } 5, 20 -- From tbargar-at-unmc.edu Mon Jan 12 10:58:03 2009 5, 20 } -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu } [192.198.54.127]) } 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n0CGw3WY025453 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 10:58:03 -0600 } 5, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) } 5, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP } id C8AFDA0076 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 10:58:02 -0600 (CST) } 5, 20 -- Received: from unmcnotes01.unmc.edu } (host-137-197-103-35.unmc.edu [137.197.103.35]) } 5, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP } id A012539804F } 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 10:58:00 -0600 (CST) } 5, 20 -- Subject: TEM:cell culture with extracellular calcium } 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- X-Mailer: } Lotus Notes Release 7.0.1 January 17, 2006 5, 20 -- } Message-ID: } {OF54FCB24C.7A37982B-ON8625753C.005CDA02-8625753C.005D334C-at-unmc.edu} } 5, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 5, 20 -- Date: } Mon, 12 Jan 2009 10:59:08 -0600 5, 20 -- X-MIMETrack: } Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at } 01/12/2009 10:59:09 5, 20 -- AM 5, 20 -- MIME-Version: 1.0 } 5, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 26 -- From DusevichV-at-umkc.edu Mon Jan 12 14:15:43 2009 8, 26 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0CKFhDZ009316 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 14:15:43 -0600 8, 26 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Mon, 12 Jan 2009 14:15:42 -0600 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: RE: [Microscopy] TEM:cell culture with extracellular calcium 8, 26 -- Date: Mon, 12 Jan 2009 14:15:42 -0600 8, 26 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AC-at-KC-MSX1.kc.umkc.edu} 8, 26 -- In-Reply-To: {200901121658.n0CGwj2i026350-at-ns.microscopy.com} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: [Microscopy] TEM:cell culture with extracellular calcium 8, 26 -- thread-index: Acl01wj0f9lD3FrtTi+bEOThxFeWogAGlcKA 8, 26 -- References: {200901121658.n0CGwj2i026350-at-ns.microscopy.com} 8, 26 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 26 -- To: {tbargar-at-unmc.edu} , {microscopy-at-msa.microscopy.com} , 8, 26 -- {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 12 Jan 2009 20:15:42.0772 (UTC) FILETIME=[8B7A3740:01C974F2] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0CKFhDZ009316 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 23 -- From nizets2-at-yahoo.com Tue Jan 13 03:38:41 2009 24, 23 -- Received: from web110811.mail.gq1.yahoo.com (web110811.mail.gq1.yahoo.com [67.195.13.234]) 24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0D9ceIv028716 24, 23 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 03:38:40 -0600 24, 23 -- Received: (qmail 51735 invoked by uid 60001); 13 Jan 2009 09:38:39 -0000 24, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 24, 23 -- s=s1024; d=yahoo.com; 24, 23 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 24, 23 -- b=WZ/fstX2SL7fNwcND9HJOkb+HHnMQWPrIRGFtWIxC3ramQWnjPyaWoF1w0ycmEk/3l6dZqh3ozcBl9MFqDznUQbSVUIamugOS36Tkka07luWlPVRYShYPngi6gAwpvtnsXzzzgSWE4HeT2tmRL1v86xp3sC6nfcF17Pv7Fo8tgI=; 24, 23 -- X-YMail-OSG: o9XbG70VM1mt1OYnAXQRD6U2ubiXED.mno5vQWXKjf16cV.8ucVWjlSr4Z5_DRY7yb6F.tDBPyUc56zh0k9JddAjj8TS9eS2NeEaf9HOCcnSYA2B.HMxdx2tbQKgIdTdKXJdSHsYgz3duwt_WPOiOKuuU.69n1eUA6drW.RSeiccF2lXK_qbPcFGhOUXjVxbMk8ZS4BcF8P2eELKCdY58ID7WbgOGHKK 24, 23 -- Received: from [80.122.101.100] by web110811.mail.gq1.yahoo.com via HTTP; Tue, 13 Jan 2009 01:38:39 PST 24, 23 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1 24, 23 -- References: {200901122019.n0CKJcVt015884-at-ns.microscopy.com} 24, 23 -- Date: Tue, 13 Jan 2009 01:38:39 -0800 (PST) 24, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com} 24, 23 -- Subject: Re: [Microscopy] RE: TEM:cell culture with extracellular calcium 24, 23 -- To: DusevichV-at-umkc.edu 24, 23 -- Cc: microscopy-at-microscopy.com 24, 23 -- MIME-Version: 1.0 24, 23 -- Content-Type: text/plain; charset=iso-8859-1 24, 23 -- Message-ID: {849685.51720.qm-at-web110811.mail.gq1.yahoo.com} 24, 23 -- Content-Transfer-Encoding: 8bit 24, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0D9ceIv028716 ==============================End of - Headers==============================
Uranium isotopes emit both alpha particles, beta-rays and gamma-rays, the former being the most significant in terms of biological hazard [assuming it's internalised]. However even natural uranium is weakly radioactive: fissile Uranium-235 has a half life of 704 million years, U-238 4.5 billion years, and 'nasty' U-234 has a modest one of 240,000 years. When uranium is enriched for bomb production or for use as reactor fuel, it's the fissile U-235* that’s wanted. However during enrichment U-234, being somewhat similar in atomic mass, gets in with the U-235 fraction, so the left over depleted uranium [DU, used in munitions casing and EM stains] is less radioactive than even natural uranium. Uranyl salts used for EM staining are now made from depleted uranium [which offers the lowest radioactivity], but compared to enriched uranium both are relatively low in radioactivity in any case, as natural uranium is 99.3% U-238 and only 0.0055% U-234.
Depleted Uranium is about 0.6 to 0.7 times as radioactive as natural uranium as it has even more U-238 and even less of the more radioactive isotopes [i.e. the small U-234 fraction accounts for about half the radioactivity of natural uranium]. U decay emits both alpha particles and beta+gamma rays. The gamma dose will be quite small [depending on the mass of the uranyl salts in your keep], but you should be able to detect it above background with a crackle-crackle type Geiger counter. As gamma dose is a function of mass & distance, keeping the small uranyl stock bottle well away from where you sit will probably suffice [perhaps with a ubiquitous 'do not eat' label, as internal exposure after ingestion/inhalation is it's main toxicological hazard]. The critical mass of U-233/U-235 [before a runaway nuclear fission reaction occurs and things get scary] is apparently around 15 to 52kg [10 to 17cm diameter volume] - never tried it personally, but that’s a lot of U and not something a microscopist need be concerned with.
In comparison plutonium-239 has a half-life of 24,000 years [ten times faster than U-234 and 187,500 faster than U238]. Thus 1g of Pu-239 is 187,500 more radioactive than 1g of U-238. There are long range gamma-rays emitted from uranium as well as alpha-particles [the latter are only of biological concern if the U is ingested]. Weapons grade enriched uranium has an alpha-particle activity of 1.91 Bq ug-1 whereas natural uranium is 100 times lower at 0.02 Bq ug-1 [and depleted uranium will be below even this]. The gamma-ray activity will be about 40% of this [for enriched uranium]. Out of interest, enriched uranium is around 93% U-235 and 0.8% U-234 [well it was in the stuff I used], thus it is significantly more radioactive compared to natural [and depleted] uranium.
However for all practical purposes the radiation effects of depleted [and even natural] uranium can generally be ignored when compared to background radiation. This is largely the case for uranium, as being a heavy metal analogue of lead, it is very toxic to life simply as a chemical. Like lead, mercury and arsenic, uranium serves 'no useful biological function' and all life-time studies find that kidney damage from uranium ingestion probably occurs long before any radiation mutagenic effects would be seen [you only die once, and as the natural U body burden increases the heavy metal toxicity is likely to get you first].
It is generally considered that lead is far more chemically cyto-toxic than uranium [largely due to U's far lower solubility and it's quick excretion rate - although this leads to deposition in the kidney tubules that can cause kidney damage]. Like lead, it is also excreted via the hair. Lead can severely affect the nervous system and other biological pathways, but this isn't seen with uranium [it's damage to kidneys being it's main toxic effect, although authors of recent DU studies have suggested links to birth defects and there's the long running controversy over gulf war syndrome]. It seems that humans have to ingest 10s of grams of natural uranium before adverse effects are seen in the short term. Animal studies suggest far lower limits are a wise precaution though with this toxic heavy metal, as damage has been seen in other organs [e.g. in the lung after inhalation of 'insoluble' enriched UO2 particles]. Natural uranium can deposit on the bone, but the weak alpha-radiation dose is far too low to induce things like leukaemia [in all probability]. So it is probably safer to be shot with a depleted uranium bullet than a lead bullet [but both are best avoided]. Likewise lead shielding is probably potentially more toxic than the uranyl salts it would be shielding. That's not to say uranium isn't very toxic, just that soluble lead is even more toxic [hence it's ability to destroy the Roman Empire from within].
Pelco [who supply the uranyl EM stain] state in there safety sheet that a material must have a specific activity greater than 74 Becquerel per gram (Bq/g) in order to be regarded as a radioactive material. One bequeral is one disintegration per gram [a very low rate]. Uranyl Acetate sold by Ted Pella, Inc. has an activity of 10,400 disintegrations sec-1 g. Thus uranyl acetate stain it is clearly also radioactive [at 0.20 - 0.51 µCi/g], although the biological hazard from far lower gamma/beta emission rate can often be considered negligible compared to background or the alpha particle emission should it become internalised in someone.
So I'd treat uranium with caution as a toxic heavy metal chemical rather than a radioactive one [as that’s probably its greatest hazard]. Either way it's hazard is by ingestion or inhalation, so handle with care as outlined on the supplied safety sheet. External irradiation by the gamma & beta rays if fairly insignificant from depleted uranium EM stains, and generally most recommend simply storing in a metal can. It's daughter decay product: Radon gas, can cause problems in enclosed areas where uranium is abundant in the soil though.
Regards
Keith J Morris
*U-235 is an interesting isotope and as well as being fissile it can be measured in extremely minute [trace] quantities using a technique known as delayed neutron analysis.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: abesenyo-at-ibilabs.com [mailto:abesenyo-at-ibilabs.com] Sent: 09 January 2009 00:27 To: kjmorris-at-well.ox.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abesenyo-at-ibilabs.com Name: Alex Besenyo PhD
Organization: ibilabs
Title-Subject: [Filtered] uranyl compounds are alpha emitters only
Question: Question:
Is it true that the stuff we use has been somehow depleted, so that it isn't as radioactive as "real" uranyl salts? Or is this yet another old wive's tale of EM?!
Reply:
When we manufacture these compounds we purchase the raw uranium in a depleted state from the government. There is no chance for error here. We do not use natural uranium.
This means that the enrichable uranium U-235 has been removed. The then U-238 which only emitts alpha radiation is procesed.
The term "depleted" means that U-235 has been removed.
If even by the slightest chance that U235 were present then every alarm would go off in our facility because Beta and Gamma radiation is detected.
I hope this answers everybodies concerns.
Our products are sold exclusively through a distributor network and all of them have been instructed on this information.
I only responded when I saw the original post and I had to respond before it got out of control.
Really, I have the feeling that we are making an elephant out of a mouse. How many times do you have to handle how much of depleted U? 2 times a year? 10 milligrams? How do you weight your uranium salt? Do you pour all the content of the box on the bench, then take what you need? :-) As someone said, I am more concerned about the chemical toxicity following ingestion (although even in this case it is probably fewer than few) than the radiations, except if you leave the box of U salt in one pocket of your blue jeans, which I wouldn't recommend (especially if you want children later).
Alternatively, I was infinitely more concerned about the election of Bush as a president than about the radiation of depleted U. Obviously I was right :-D And there is no Bush-Hazard-Security-Agency, no rules to dispose of Bush in an environment-friendly way and no ALARE rules (as long as reasonably eligible). Well it is never too late to start. Oh, is it? :-D
----- Original Message ---- X-from: "kjmorris-at-well.ox.ac.uk" {kjmorris-at-well.ox.ac.uk} To: nizets2-at-yahoo.com Sent: Tuesday, January 13, 2009 11:12:29 AM
Hi all,
Uranium isotopes emit both alpha particles, beta-rays and gamma-rays, the former being the most significant in terms of biological hazard [assuming it's internalised]. However even natural uranium is weakly radioactive: fissile Uranium-235 has a half life of 704 million years, U-238 4.5 billion years, and 'nasty' U-234 has a modest one of 240,000 years. When uranium is enriched for bomb production or for use as reactor fuel, it's the fissile U-235* that’s wanted. However during enrichment U-234, being somewhat similar in atomic mass, gets in with the U-235 fraction, so the left over depleted uranium [DU, used in munitions casing and EM stains] is less radioactive than even natural uranium. Uranyl salts used for EM staining are now made from depleted uranium [which offers the lowest radioactivity], but compared to enriched uranium both are relatively low in radioactivity in any case, as natural uranium is 99.3% U-238 and only 0.0055% U-234.
Depleted Uranium is about 0.6 to 0.7 times as radioactive as natural uranium as it has even more U-238 and even less of the more radioactive isotopes [i.e. the small U-234 fraction accounts for about half the radioactivity of natural uranium]. U decay emits both alpha particles and beta+gamma rays. The gamma dose will be quite small [depending on the mass of the uranyl salts in your keep], but you should be able to detect it above background with a crackle-crackle type Geiger counter. As gamma dose is a function of mass & distance, keeping the small uranyl stock bottle well away from where you sit will probably suffice [perhaps with a ubiquitous 'do not eat' label, as internal exposure after ingestion/inhalation is it's main toxicological hazard]. The critical mass of U-233/U-235 [before a runaway nuclear fission reaction occurs and things get scary] is apparently around 15 to 52kg [10 to 17cm diameter volume] - never tried it personally, but that’s a lot of U and not something a microscopist need be concerned with.Â
In comparison plutonium-239 has a half-life of 24,000 years [ten times faster than U-234 and 187,500 faster than U238]. Thus 1g of Pu-239 is 187,500 more radioactive than 1g of U-238. There are long range gamma-rays emitted from uranium as well as alpha-particles [the latter are only of biological concern if the U is ingested]. Weapons grade enriched uranium has an alpha-particle activity of 1.91 Bq ug-1 whereas natural uranium is 100 times lower at 0.02 Bq ug-1 [and depleted uranium will be below even this]. The gamma-ray activity will be about 40% of this [for enriched uranium]. Out of interest, enriched uranium is around 93% U-235 and 0.8% U-234 [well it was in the stuff I used], thus it is significantly more radioactive compared to natural [and depleted] uranium.
However for all practical purposes the radiation effects of depleted [and even natural] uranium can generally be ignored when compared to background radiation. This is largely the case for uranium, as being a heavy metal analogue of lead, it is very toxic to life simply as a chemical. Like lead, mercury and arsenic, uranium serves 'no useful biological function' and all life-time studies find that kidney damage from uranium ingestion probably occurs long before any radiation mutagenic effects would be seen [you only die once, and as the natural U body burden increases the heavy metal toxicity is likely to get you first].
It is generally considered that lead is far more chemically cyto-toxic than uranium [largely due to U's far lower solubility and it's quick excretion rate - although this leads to deposition in the kidney tubules that can cause kidney damage]. Like lead, it is also excreted via the hair. Lead can severely affect the nervous system and other biological pathways, but this isn't seen with uranium [it's damage to kidneys being it's main toxic effect, although authors of recent DU studies have suggested links to birth defects and there's the long running controversy over gulf war syndrome]. It seems that humans have to ingest 10s of grams of natural uranium before adverse effects are seen in the short term. Animal studies suggest far lower limits are a wise precaution though with this toxic heavy metal, as damage has been seen in other organs [e.g. in the lung after inhalation of 'insoluble' enriched UO2 particles]. Natural uranium can deposit on the bone, but the weak alpha-radiation dose is far too low to induce things like leukaemia [in all probability]. So it is probably safer to be shot with a depleted uranium bullet than a lead bullet [but both are best avoided]. Likewise lead shielding is probably potentially more toxic than the uranyl salts it would be shielding. That's not to say uranium isn't very toxic, just that soluble lead is even more toxic [hence it's ability to destroy the Roman Empire from within].
Pelco [who supply the uranyl EM stain] state in there safety sheet that a material must have a specific activity greater than 74 Becquerel per gram (Bq/g) in order to be regarded as a radioactive material. One bequeral is one disintegration per gram [a very low rate]. Uranyl Acetate sold by Ted Pella, Inc. has an activity of 10,400 disintegrations sec-1 g. Thus uranyl acetate stain it is clearly also radioactive [at 0.20 - 0.51 µCi/g], although the biological hazard from far lower gamma/beta emission rate can often be considered negligible compared to background or the alpha particle emission should it become internalised in someone.
So I'd treat uranium with caution as a toxic heavy metal chemical rather than a radioactive one [as that’s probably its greatest hazard]. Either way it's hazard is by ingestion or inhalation, so handle with care as outlined on the supplied safety sheet. External irradiation by the gamma & beta rays if fairly insignificant from depleted uranium EM stains, and generally most recommend simply storing in a metal can. It's daughter decay product: Radon gas, can cause problems in enclosed areas where uranium is abundant in the soil though.
Regards
Keith J Morris
*U-235 is an interesting isotope and as well as being fissile it can be measured in extremely minute [trace] quantities using a technique known as delayed neutron analysis.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: abesenyo-at-ibilabs.com [mailto:abesenyo-at-ibilabs.com] Sent: 09 January 2009 00:27 To: kjmorris-at-well.ox.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abesenyo-at-ibilabs.com Name: Alex Besenyo PhD
Organization: ibilabs
Title-Subject: [Filtered] uranyl compounds are alpha emitters only
Question: Question:
Is it true that the stuff we use has been somehow depleted, so that it isn't as radioactive as "real" uranyl salts? Or is this yet another old wive's tale of EM?!
Reply:
When we manufacture these compounds we purchase the raw uranium in a depleted state from the government. There is no chance for error here. We do not use natural uranium.
This means that the enrichable uranium U-235 has been removed. The then U-238 which only emitts alpha radiation is procesed.
The term "depleted" means that U-235 has been removed.
If even by the slightest chance that U235 were present then every alarm would go off in our facility because Beta and Gamma radiation is detected.
I hope this answers everybodies concerns.
Our products are sold exclusively through a distributor network and all of them have been instructed on this information.
I only responded when I saw the original post and I had to respond before it got out of control.
Sincerely Alex Besenyo PhD
 Login Host: 74.173.69.139 ---------------------------------------------------------------------------
==============================Original Headers============================== 17, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:19:52 2009 17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) 17, 11 --    by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n090Jp6S032218 17, 11 --    for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52 -0600 17, 11 -- Mime-Version: 1.0 17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]} 17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600 17, 11 -- To: microscopy-at-microscopy.com 17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver) 17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only 17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 39, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jan 13 04:05:07 2009 39, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 39, 23 --    by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DA56tP012959 39, 23 --    for {Microscopy-at-Microscopy.Com} ; Tue, 13 Jan 2009 04:05:06 -0600 39, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 39, 23 --    by morse.well.ox.ac.uk with esmtp (Exim 4.52) 39, 23 --    id 1LMg8v-0006zz-ST 39, 23 --    for Microscopy-at-Microscopy.Com; Tue, 13 Jan 2009 10:05:06 +0000 39, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 39, 23 -- To: {Microscopy-at-Microscopy.Com} 39, 23 -- References: {200901090027.n090RFFt021207-at-ns.microscopy.com} 39, 23 -- Subject: RE: [Microscopy] viaWWW:Yes uranyl compounds do emit few gammas, betas as well 39, 23 -- Date: Tue, 13 Jan 2009 10:05:06 -0000 39, 23 -- Message-ID: {08E534FB0C6245979400D75365C7BAAF-at-CytoWhizz} 39, 23 -- MIME-Version: 1.0 39, 23 -- Content-Type: text/plain; 39, 23 --    charset="iso-8859-1" 39, 23 -- X-Mailer: Microsoft Office Outlook 11 39, 23 -- Thread-Index: Aclx8QYXX4MpJfSGTamN32m5ifY3bwAVUa0g 39, 23 -- In-Reply-To: {200901090027.n090RFFt021207-at-ns.microscopy.com} 39, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 39, 23 -- Content-Transfer-Encoding: 8bit 39, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DA56tP012959 ==============================End of - Headers==============================
==============================Original Headers============================== 57, 22 -- From nizets2-at-yahoo.com Tue Jan 13 07:11:23 2009 57, 22 -- Received: from web110816.mail.gq1.yahoo.com (web110816.mail.gq1.yahoo.com [67.195.13.239]) 57, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0DDBMqm009455 57, 22 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 07:11:22 -0600 57, 22 -- Received: (qmail 20923 invoked by uid 60001); 13 Jan 2009 13:11:22 -0000 57, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 57, 22 -- s=s1024; d=yahoo.com; 57, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 57, 22 -- b=AkrMz6GOq46CXKK5pOahDO6ZAfPPx48th9zUS4KULg0103NI1ifS/Zu4WQcIq9wOWLKYF3OO1YZ0dt85zbmE8FcB9bLkv6pP9Hlqm4LU3TxczJUrh6vJ831tTsMFJTsVcPu3d3VnEVudGcCV46iJvZ4S2VZXnhaGLhsxvsG0Tb0=; 57, 22 -- X-YMail-OSG: Zk.PogUVM1kVvQOYpqeXXnlFegUuBHQSs4XK0E87Nd8rz9zPBGBDGggYS18nRO3I5qe6z1y4aJX8L.3g7JMsH0hfefy8cMoX5gdFUcEdg_IpPdXzZl8e9M0hRcD65TsRPyAVZGRvqSOU_m7no6fnPXvwOLR1U7o578vTTjlpHMGglKoYzYIdJ7UAMwm7jQChIxQJCS8dDKLBbKv4ru.Vo4qr7pLEJ.jK 57, 22 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Tue, 13 Jan 2009 05:11:22 PST 57, 22 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1 57, 22 -- References: {200901131012.n0DACTSC020042-at-ns.microscopy.com} 57, 22 -- Date: Tue, 13 Jan 2009 05:11:22 -0800 (PST) 57, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 57, 22 -- Subject: uranyl compounds do emit few gammas, betas as well - Who cares? 57, 22 -- To: microscopy-at-microscopy.com 57, 22 -- MIME-Version: 1.0 57, 22 -- Content-Type: text/plain; charset=utf-8 57, 22 -- Message-ID: {380151.20813.qm-at-web110816.mail.gq1.yahoo.com} 57, 22 -- Content-Transfer-Encoding: 8bit 57, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DDBMqm009455 ==============================End of - Headers==============================
Folks: Old photo/darkroom supplies, etc., may find a happy home in art schools. I have a young artist friend who is taking a photography class and the instructor is insisting that she start with film in order to understand the fundamentals of photography - particularly for B/W. Without getting into arguments about log/linear behavior, benefits of one over the other, etc., the point is that these folks may be able to utilize film supplies and are likely to be very appreciative of a cheap/free source. They may not have much use for the Type xxx film, but some of the other supplies could be valuable. Best Regards, Bill William A. Heeschen Microscopy, Digital Imaging 1897 Bldg, E-84 Dow Chemical Midland, MI 48667 mailto:waheeschen-at-dow.com
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Monday, January 12, 2009 5:04 PM To: Heeschen, Bill (WA)
Greetings
This is a message I never thought I would be sending.
We are getting out of the film and paper photo business, and have a lot of 'stuff' to give away or send to the landfill. Anyone with questions about whether digital imaging for EM is for real, let this be your wake-up call.
Most of what we have is old, probably not worth the cost to ship, unless you are desperate, or curious.
We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572. I don't even know what some of this was used for, if it rings your bell, let me know and I can tell you more.
We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4 x5 sizes.
A little 4463 and a ton of SO-163. The SO-163 is old.
Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan.
Most of this junk has been in a freezer. Some is pretty old and past its expiration date, but if you are interested, let me know and we can try to work something out. If I don't hear anything in the next week or so, its outa here.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 14, 42 -- From jkrupp-at-deltacollege.edu Mon Jan 12 15:59:23 2009 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0CLxMKe006917 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 15:59:23 -0600 14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 14, 42 -- by localhost (Email Security Appliance) with SMTP id 7D4C918E05E 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 21:34:33 +0000 (GMT) 14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 14, 42 -- by mailin.deltacollege.edu (Email Security Appliance) with ESMTP id 726D520CAFD 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 21:34:33 +0000 (GMT) 14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 14, 42 -- with ESMTP id 44930245 for Microscopy-at-microscopy.com; Mon, 12 Jan 2009 13:59:19 -0800 14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 14, 42 -- ESMTP id KDDOD300.CM0 for {Microscopy-at-microscopy.com} ; Mon, 12 14, 42 -- Jan 2009 13:43:51 -0800 14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 13E398F74D38 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:59:19 -0800 (PST) 14, 42 -- X-Virus-Scanned: amavisd-new at 14, 42 -- X-Spam-Flag: NO 14, 42 -- X-Spam-Score: -2.324 14, 42 -- X-Spam-Level: 14, 42 -- X-Spam-Status: No, score=-2.324 tagged_above=-10 required=6 tests=[AWL=0.175, 14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] 14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 14, 42 -- with ESMTP id sAJT+MZ3mFOx for {Microscopy-at-microscopy.com} ; 14, 42 -- Mon, 12 Jan 2009 13:59:18 -0800 (PST) 14, 42 -- Received: from [172.20.4.106] (unknown [172.20.4.106]) 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id AEBE88F74D32 14, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan 2009 13:59:18 -0800 (PST) 14, 42 -- Message-Id: {7C9F6B4C-B8AF-43D7-86A6-495112B71078-at-deltacollege.edu} 14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 14, 42 -- To: Microscopy-at-microscopy.com 14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 42 -- Content-Transfer-Encoding: 7bit 14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 14, 42 -- Subject: Old film etc 14, 42 -- Date: Mon, 12 Jan 2009 13:59:18 -0800 14, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 21, 34 -- From WAHeeschen-at-dow.com Tue Jan 13 08:36:46 2009 21, 34 -- Received: from mail85.messagelabs.com (mail85.messagelabs.com [216.82.241.211]) 21, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DEaks3030832 21, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 08:36:46 -0600 21, 34 -- X-VirusChecked: Checked 21, 34 -- X-Env-Sender: WAHeeschen-at-dow.com 21, 34 -- X-Msg-Ref: server-3.tower-85.messagelabs.com!1231857396!33256808!9 21, 34 -- X-StarScan-Version: 6.0.0; banners=-,-,- 21, 34 -- X-Originating-IP: [216.99.65.22] 21, 34 -- Received: (qmail 24830 invoked from network); 13 Jan 2009 14:36:45 -0000 21, 34 -- Received: from mail1.dow.com (HELO USMDLMDOWS001.dow.com) (216.99.65.22) 21, 34 -- by server-3.tower-85.messagelabs.com with RC4-SHA encrypted SMTP; 13 Jan 2009 14:36:45 -0000 21, 34 -- Received: from USMDLMDOWX026.dow.com ([163.198.215.57]) by USMDLMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830); 21, 34 -- Tue, 13 Jan 2009 09:36:37 -0500 21, 34 -- Content-class: urn:content-classes:message 21, 34 -- MIME-Version: 1.0 21, 34 -- Content-Type: text/plain; 21, 34 -- charset="US-ASCII" 21, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 34 -- Subject: RE: [Microscopy] Old film etc 21, 34 -- Date: Tue, 13 Jan 2009 09:36:37 -0500 21, 34 -- Message-ID: {9FAC91DDE67EF3448238E5E33D3E5AEF01C6B129-at-USMDLMDOWX026.dow.com} 21, 34 -- In-Reply-To: {200901122204.n0CM47gR016422-at-ns.microscopy.com} 21, 34 -- X-MS-Has-Attach: 21, 34 -- X-MS-TNEF-Correlator: 21, 34 -- Thread-Topic: [Microscopy] Old film etc 21, 34 -- Thread-Index: Acl1AcbMkH3Log7TSe6nWCmmqyEhZgAiFHUg 21, 34 -- References: {200901122204.n0CM47gR016422-at-ns.microscopy.com} 21, 34 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com} 21, 34 -- To: {jkrupp-at-deltacollege.edu} 21, 34 -- Cc: {Microscopy-at-microscopy.com} 21, 34 -- X-OriginalArrivalTime: 13 Jan 2009 14:36:37.0775 (UTC) FILETIME=[5753C9F0:01C9758C] 21, 34 -- Content-Transfer-Encoding: 8bit 21, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DEaks3030832 ==============================End of - Headers==============================
Good point, although not necessarily the case. The Art and journalism deparments here have gone completely digital. Even strictly art photography, the kind that uses high silver content paper and so forth. They didn't even want a Durst enlarger. Anybody on the list looking for a Durst Laborator enlarger in excellent condition? With printing easel, extra lenses, etc.?
Phil
} } Folks: } Old photo/darkroom supplies, etc., may find a happy home in art schools. } I have a young artist friend who is taking a photography class and the } instructor is insisting that she start with film in order to understand } the fundamentals of photography - particularly for B/W. Without getting } into arguments about log/linear behavior, benefits of one over the } other, etc., the point is that these folks may be able to utilize film } supplies and are likely to be very appreciative of a cheap/free source. } They may not have much use for the Type xxx film, but some of the other } supplies could be valuable. } Best Regards, } Bill } William A. Heeschen } Microscopy, Digital Imaging } 1897 Bldg, E-84 } Dow Chemical } Midland, MI 48667 } mailto:waheeschen-at-dow.com } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: Monday, January 12, 2009 5:04 PM } To: Heeschen, Bill (WA) } Subject: [Microscopy] Old film etc } --- } } Greetings } } This is a message I never thought I would be sending. } } We are getting out of the film and paper photo business, and have a } lot of 'stuff' to give away or send to the landfill. Anyone with } questions about whether digital imaging for EM is for real, let this } be your wake-up call. } } Most of what we have is old, probably not worth the cost to ship, } unless you are desperate, or curious. } } We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572. } I don't even know what some of this was used for, if it rings your } bell, let me know and I can tell you more. } } We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4 } x5 sizes. } } A little 4463 and a ton of SO-163. The SO-163 is old. } } Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan. } } Most of this junk has been in a freezer. Some is pretty old and past } its expiration date, but if you are interested, let me know and we can } try to work something out. If I don't hear anything in the next week } or so, its outa here. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 25 -- From oshel1pe-at-cmich.edu Tue Jan 13 08:59:55 2009 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DExs4e026270 4, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 08:59:55 -0600 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n0DExhQX000442 4, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 09:59:53 -0500 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 25 -- Tue, 13 Jan 2009 09:58:51 -0500 4, 25 -- Mime-Version: 1.0 4, 25 -- Message-Id: {f06240805c5925b610675-at-[141.209.160.249]} 4, 25 -- In-Reply-To: {200901131442.n0DEgQst004798-at-ns.microscopy.com} 4, 25 -- References: {200901131442.n0DEgQst004798-at-ns.microscopy.com} 4, 25 -- Date: Tue, 13 Jan 2009 09:58:49 -0500 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 25 -- Subject: [Microscopy] RE: Old film etc 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 25 -- X-OriginalArrivalTime: 13 Jan 2009 14:58:51.0969 (UTC) FILETIME=[7291A310:01C9758F] 4, 25 -- X-Canit-CHI2: 0.00 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 25 -- X-CanItPRO-Stream: default 4, 25 -- X-Canit-Stats-ID: 7380497 - 047461f10392 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
No, it does not. Can you read other, more meaningful postings?
Vladimir
that we have a material which emits alpha, beta } and gamma rays. I think the original labeling for uranyl } compounds which said "alpha emitter" should be changed. } Especially, for the reason that there might be microscopists } who are using uranyl compounds in their labs but, do not } follow the Microscopy List. } } Is there anybody in this group who is in a position and } willing to contact Nuclear Regulatory Commission on this subject? } } Regards, } Ayten. } } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } =========================== } } } On Mon, Jan 12, 2009 at 8:26 PM, {tivol-at-caltech.edu} wrote: } } } } } } } } } -------------------------------------------------------------- } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } -------------------------------------------------------------- } -------------- } } } } } } On Jan 9, 2009, at 4:07 PM, beaurega-at-westol.com wrote: } } } } } Someone strongly pointed out that U is an alpha emitter, } not a gamma } } } emitter, and I was not reading gamma radiation (but I was). You } } } pointed } } } out that it was the decay impurities that were the gamma emitters } } } and that } } } was what I was reading on my counter. I agree that U-238 } and DU are } } } still } } } radioactive. } } } } } Here's my favorite. "depleted uranium is 40% less radioactive than } } } natural } } } uranium." That means 60% of the radioactivity is still } there. I am } } } not } } } sure that includes gamma but probably. } } } } } } Dear Paul, } } Since the activities of the U isotopes are inversely } proportional to } } their half-lives, and since the half lives of U235 and U234 } are about } } 7 and 20,000 times shorter respectively than U238's, the amount of } } radiation from U234 is about equal to that from U238, and that from } } U235 is about 5% of that from the others, so your quote that DU has } } only 60% the activity of natural U checks out. I pointed out that } } ~1/4 of the U238 decays are to an excited state of Th234, } which is ~50 } } keV above the ground state, so pure U238 will produce some } low-energy } } gammas, as will many of the daughters. The bottom line is } that direct } } measurements performed correctly don't lie, so they are the best way } } to settle this issue once and for all. } } Yours, } } Bill Tivol, PhD } } EM Scientist } } Ultrafast EM Facility } } Noyes Laboratory, MC 127-72 } } California Institute of Technology } } Pasadena CA 91125 } } (626) 395-8833 } } tivol-at-caltech.edu } } } } } } ==============================Original } Headers============================== } 8, 32 -- From celikaktas-at-gmail.com Tue Jan 13 01:21:11 2009 } 8, 32 -- Received: from mu-out-0910.google.com } (mu-out-0910.google.com [209.85.134.189]) } 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n0D7LAJI000663 } 8, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan } 2009 01:21:10 -0600 } 8, 32 -- Received: by mu-out-0910.google.com with SMTP id } w9so6630873mue.0 } 8, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Jan } 2009 23:21:09 -0800 (PST) } 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 32 -- d=gmail.com; s=gamma; } 8, 32 -- } h=domainkey-signature:mime-version:received:date:message-id:subject } 8, 32 -- :from:to:content-type:content-transfer-encoding; } 8, 32 -- bh=wjfEMkzAxlZHP279oRoyEi2BHmNZ5qmvW9E+/LODEuc=; } 8, 32 -- } b=E1VS580eiZKvpEWh5Swi392D4XXRNLvDCVuFEuSp9UqhBV1JvfuOvMIDnVoR1vNznc } 8, 32 -- } r5L1Z0TtXqt7mJXYCYcuaRGapCY8uHGnXv6W+VlNuOkBmNIfp97CLppTrQ9q9ppekELu } 8, 32 -- kwwJ7gZg97W5DDFnjeF/OxGaHUrQ6UD82ohLY= } 8, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 8, 32 -- d=gmail.com; s=gamma; } 8, 32 -- } h=mime-version:date:message-id:subject:from:to:content-type } 8, 32 -- :content-transfer-encoding; } 8, 32 -- } b=ntMPorKNV/TSgRwuNzbJnEnwEgRXpiOCZr8l7jnSDPeacm7aCKVfux+bNhJKw49bri } 8, 32 -- } JcAVl8XZn7UmecRQPwL7o5bltCdYS+8v59joM0sIvDoKUmY8PGV2YorAmmiQ6/M6j/ll } 8, 32 -- JGVoOwQH4/WN2HEF0ny5gehH0tcdQgk08a7+Q= } 8, 32 -- MIME-Version: 1.0 } 8, 32 -- Received: by 10.103.217.7 with SMTP id } u7mr3452314muq.125.1231831269725; Mon, } 8, 32 -- 12 Jan 2009 23:21:09 -0800 (PST) } 8, 32 -- Date: Tue, 13 Jan 2009 10:21:09 +0300 } 8, 32 -- Message-ID: } {1075c5c10901122321l5c772174r4fedda4b0656a5-at-mail.gmail.com} } 8, 32 -- Subject: Re: [Microscopy] Updating Label?... viaWWW: } uranyl compounds are } 8, 32 -- alpha emitters } 8, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} } 8, 32 -- To: microscopy {Microscopy-at-microscopy.com} } 8, 32 -- Content-Type: text/plain; charset=UTF-8 } 8, 32 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 25 -- From DusevichV-at-umkc.edu Tue Jan 13 09:12:59 2009 7, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.155]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DFCxeO009341 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Jan 2009 09:12:59 -0600 7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 25 -- Tue, 13 Jan 2009 09:12:59 -0600 7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 25 -- Content-class: urn:content-classes:message 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="us-ascii" 7, 25 -- Subject: RE: [Microscopy] Re: Updating Label?... viaWWW: uranyl compounds are 7, 25 -- Date: Tue, 13 Jan 2009 09:12:59 -0600 7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7AF-at-KC-MSX1.kc.umkc.edu} 7, 25 -- In-Reply-To: {200901130722.n0D7M7G5001697-at-ns.microscopy.com} 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- Thread-Topic: [Microscopy] Re: Updating Label?... viaWWW: uranyl compounds are 7, 25 -- thread-index: Acl1T6VuVvBucRIWSfWbcChvYMJnGgAQY0fQ 7, 25 -- References: {200901130722.n0D7M7G5001697-at-ns.microscopy.com} 7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 25 -- To: {Microscopy-at-microscopy.com} 7, 25 -- X-OriginalArrivalTime: 13 Jan 2009 15:12:59.0426 (UTC) FILETIME=[6BB15020:01C97591] 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0DFCxeO009341 ==============================End of - Headers==============================
From mathewokon-at-sify.com Tue Jan 13 10:44:28 2009 Return-Path: {mathewokon-at-sify.com} Received: from vetbrands.com.br (smtp-gw.vetbrands.com.br [201.63.46.162]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0DGiRNe028666; Tue, 13 Jan 2009 10:44:28 -0600 BrmaOutput: [41.222.67.140] Received: from User ([41.222.67.140]) (authenticated bits=0) by vetbrands.com.br (8.12.11.20060308/8.12.11) with ESMTP id n0DC3hlv024740; Tue, 13 Jan 2009 10:03:47 -0200 Message-Id: {200901131203.n0DC3hlv024740-at-vetbrands.com.br} Reply-To: {mathew_okon1-at-yahoo.es}
Indeed, from Pelco's measurements of their solution I estimate that if you held a 25g bottle of their uranyl acetate against your skin for an entire year you would receive about 3.5 times the annual dose* you would get from background radiation sources [over the same year] - about 1,300 mRem. This would largely be from the gamma radiation [as you can assume the beta-rays and alpha particles are blocked by the glass jar, i.e. you kept the lid on, and your skin surface].
Keith
*Annual dose assumed to be 360 mRem [18% man made + 82% natural]. A radiation worker is allowed 5,000 mRem maximum occupational exposure.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 13 January 2009 13:19 To: kjmorris-at-well.ox.ac.uk
Dear all!
Really, I have the feeling that we are making an elephant out of a mouse. How many times do you have to handle how much of depleted U? 2 times a year? 10 milligrams? How do you weight your uranium salt? Do you pour all the content of the box on the bench, then take what you need? :-) As someone said, I am more concerned about the chemical toxicity following ingestion (although even in this case it is probably fewer than few) than the radiations, except if you leave the box of U salt in one pocket of your blue jeans, which I wouldn't recommend (especially if you want children later).
Alternatively, I was infinitely more concerned about the election of Bush as a president than about the radiation of depleted U. Obviously I was right :-D And there is no Bush-Hazard-Security-Agency, no rules to dispose of Bush in an environment-friendly way and no ALARE rules (as long as reasonably eligible). Well it is never too late to start. Oh, is it? :-D
----- Original Message ---- X-from: "kjmorris-at-well.ox.ac.uk" {kjmorris-at-well.ox.ac.uk} To: nizets2-at-yahoo.com Sent: Tuesday, January 13, 2009 11:12:29 AM
Hi all,
Uranium isotopes emit both alpha particles, beta-rays and gamma-rays, the former being the most significant in terms of biological hazard [assuming it's internalised]. However even natural uranium is weakly radioactive: fissile Uranium-235 has a half life of 704 million years, U-238 4.5 billion years, and 'nasty' U-234 has a modest one of 240,000 years. When uranium is enriched for bomb production or for use as reactor fuel, it's the fissile U-235* that’s wanted. However during enrichment U-234, being somewhat similar in atomic mass, gets in with the U-235 fraction, so the left over depleted uranium [DU, used in munitions casing and EM stains] is less radioactive than even natural uranium. Uranyl salts used for EM staining are now made from depleted uranium [which offers the lowest radioactivity], but compared to enriched uranium both are relatively low in radioactivity in any case, as natural uranium is 99.3% U-238 and only 0.0055% U-234.
Depleted Uranium is about 0.6 to 0.7 times as radioactive as natural uranium as it has even more U-238 and even less of the more radioactive isotopes [i.e. the small U-234 fraction accounts for about half the radioactivity of natural uranium]. U decay emits both alpha particles and beta+gamma rays. The gamma dose will be quite small [depending on the mass of the uranyl salts in your keep], but you should be able to detect it above background with a crackle-crackle type Geiger counter. As gamma dose is a function of mass & distance, keeping the small uranyl stock bottle well away from where you sit will probably suffice [perhaps with a ubiquitous 'do not eat' label, as internal exposure after ingestion/inhalation is it's main toxicological hazard]. The critical mass of U-233/U-235 [before a runaway nuclear fission reaction occurs and things get scary] is apparently around 15 to 52kg [10 to 17cm diameter volume] - never tried it personally, but that’s a lot of U and not something a microscopist need be concerned with.Â
In comparison plutonium-239 has a half-life of 24,000 years [ten times faster than U-234 and 187,500 faster than U238]. Thus 1g of Pu-239 is 187,500 more radioactive than 1g of U-238. There are long range gamma-rays emitted from uranium as well as alpha-particles [the latter are only of biological concern if the U is ingested]. Weapons grade enriched uranium has an alpha-particle activity of 1.91 Bq ug-1 whereas natural uranium is 100 times lower at 0.02 Bq ug-1 [and depleted uranium will be below even this]. The gamma-ray activity will be about 40% of this [for enriched uranium]. Out of interest, enriched uranium is around 93% U-235 and 0.8% U-234 [well it was in the stuff I used], thus it is significantly more radioactive compared to natural [and depleted] uranium.
However for all practical purposes the radiation effects of depleted [and even natural] uranium can generally be ignored when compared to background radiation. This is largely the case for uranium, as being a heavy metal analogue of lead, it is very toxic to life simply as a chemical. Like lead, mercury and arsenic, uranium serves 'no useful biological function' and all life-time studies find that kidney damage from uranium ingestion probably occurs long before any radiation mutagenic effects would be seen [you only die once, and as the natural U body burden increases the heavy metal toxicity is likely to get you first].
It is generally considered that lead is far more chemically cyto-toxic than uranium [largely due to U's far lower solubility and it's quick excretion rate - although this leads to deposition in the kidney tubules that can cause kidney damage]. Like lead, it is also excreted via the hair. Lead can severely affect the nervous system and other biological pathways, but this isn't seen with uranium [it's damage to kidneys being it's main toxic effect, although authors of recent DU studies have suggested links to birth defects and there's the long running controversy over gulf war syndrome]. It seems that humans have to ingest 10s of grams of natural uranium before adverse effects are seen in the short term. Animal studies suggest far lower limits are a wise precaution though with this toxic heavy metal, as damage has been seen in other organs [e.g. in the lung after inhalation of 'insoluble' enriched UO2 particles]. Natural uranium can deposit on the bone, but the weak alpha-radiation dose is far too low to induce things like leukaemia [in all probability]. So it is probably safer to be shot with a depleted uranium bullet than a lead bullet [but both are best avoided]. Likewise lead shielding is probably potentially more toxic than the uranyl salts it would be shielding. That's not to say uranium isn't very toxic, just that soluble lead is even more toxic [hence it's ability to destroy the Roman Empire from within].
Pelco [who supply the uranyl EM stain] state in there safety sheet that a material must have a specific activity greater than 74 Becquerel per gram (Bq/g) in order to be regarded as a radioactive material. One bequeral is one disintegration per gram [a very low rate]. Uranyl Acetate sold by Ted Pella, Inc. has an activity of 10,400 disintegrations sec-1 g. Thus uranyl acetate stain it is clearly also radioactive [at 0.20 - 0.51 µCi/g], although the biological hazard from far lower gamma/beta emission rate can often be considered negligible compared to background or the alpha particle emission should it become internalised in someone.
So I'd treat uranium with caution as a toxic heavy metal chemical rather than a radioactive one [as that’s probably its greatest hazard]. Either way it's hazard is by ingestion or inhalation, so handle with care as outlined on the supplied safety sheet. External irradiation by the gamma & beta rays if fairly insignificant from depleted uranium EM stains, and generally most recommend simply storing in a metal can. It's daughter decay product: Radon gas, can cause problems in enclosed areas where uranium is abundant in the soil though.
Regards
Keith J Morris
*U-235 is an interesting isotope and as well as being fissile it can be measured in extremely minute [trace] quantities using a technique known as delayed neutron analysis.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: abesenyo-at-ibilabs.com [mailto:abesenyo-at-ibilabs.com] Sent: 09 January 2009 00:27 To: kjmorris-at-well.ox.ac.uk
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Email: abesenyo-at-ibilabs.com Name: Alex Besenyo PhD
Organization: ibilabs
Title-Subject: [Filtered] uranyl compounds are alpha emitters only
Question: Question:
Is it true that the stuff we use has been somehow depleted, so that it isn't as radioactive as "real" uranyl salts? Or is this yet another old wive's tale of EM?!
Reply:
When we manufacture these compounds we purchase the raw uranium in a depleted state from the government. There is no chance for error here. We do not use natural uranium.
This means that the enrichable uranium U-235 has been removed. The then U-238 which only emitts alpha radiation is procesed.
The term "depleted" means that U-235 has been removed.
If even by the slightest chance that U235 were present then every alarm would go off in our facility because Beta and Gamma radiation is detected.
I hope this answers everybodies concerns.
Our products are sold exclusively through a distributor network and all of them have been instructed on this information.
I only responded when I saw the original post and I had to respond before it got out of control.
They tried examination by SEM but needed better resolution than we could achieve with our conventional, older SEMs. I considered using HF to digest the sand grain but they nixed the idea since they wanted to show orientation of tubules relative to the surface. Oh, well.
We did get sections using a diamond knife but the nanotubes appeared to be round globes rather than tubes. I'm uncertain if the embedding somehow messed them up or, more probably, nanotubes had not formed and we were looking at sphreoidal materials instead.
John
} Hi John! Sorry for the very late reply but I was } in well-earned holidays (and yes this year they } were long). I often cut hard particles under 1 } µm in size with little problem to the knife. It } is true though that the particles move in the } resin, leaving holes. I suppose that from a } given size the damage to the knife becomes a } real problem. Your particles are probably much } bigger, which may be a big deal to cut. Now my } personal opinion: I wonder why TEM would be more } appropriate than SEM to study the distribution } of nanotubes at the surface of sand particles. } And finally, my usual crazy idea: why not try to } "digest" the sand, leaving only the nanotubes? } You cannot analyse the interface between } nanotubes and sand anymore, but if the nanotubes } are dense enough their organization may be } conserved. It is better than nothing! Let's } suppose that the nanotubes are made of carbon, } they are probably inert to any treatment. } Following my fellow colleagues (chemists and } geologists), digesting sand is not an easy task } though. They suggest something like concentrated } NaOH. Just my 2 cents, not a lot worth. Stéphane } ----- Original Message ---- From: } "bozzola-at-siu.edu" {bozzola-at-siu.edu} To: } nizets2-at-yahoo.com Sent: Saturday, December 20, } 2008 12:22:53 AM Subject: [Microscopy] TEM: } sectioning sand } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America To } Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } I've cut some hard specimens over the years but } never sand. We have a researcher who wishes to } look at a section of a sand grain to study the } distribution of nanotubes on the surface. Any } suggestions on sectioning a grain of sand? } Here's what I am planning: embedding in hard } Spurr resin old, 50 degree diamond knife 2 } mm/sec cutting speed Thanks, JB -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } John J. Bozzola, Ph.D., Director Integrated } Microscopy & Graphics Expertise (IMAGE) Southern } Illinois University 750 Communications Drive - } MC 4402 Carbondale, IL 62901 Telephone: } 618-453-3730 } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } ==============================Original } Headers============================== 8, 17 -- } From bozzola-at-siu.edu Fri Dec 19 17:19:43 2008 8, } 17 -- Received: from cstmta3.siu.edu } (cstmta3.siu.edu [131.230.1.3]) 8, 17 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id mBJNJhJX008553 8, 17 -- for } {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 } 17:19:43 -0600 8, 17 -- Received: from } [131.230.177.136] (ws177136.microscope.siu.edu } [131.230.177.136]) 8, 17 -- by } cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with } ESMTP id mBJNJe5s027174 8, 17 -- for } {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 } 17:19:42 -0600 (CST) 8, 17 -- Mime-Version: 1.0 } 8, 17 -- Message-Id: } {a06240812c571db4cd407-at-[131.230.177.136]} 8, 17 } -- Date: Fri, 19 Dec 2008 17:19:36 -0600 8, 17 } -- To: Microscopy-at-microscopy.com 8, 17 -- From: } "John J. Bozzola" {bozzola-at-siu.edu} 8, 17 -- } Subject: TEM: sectioning sand 8, 17 -- } Content-Type: text/plain; charset="us-ascii" ; } format="flowed" 8, 17 -- X-Spam-Score: 0.00% 8, } 17 -- X-MASF: 0.00% 8, 17 -- X-Whitelist: 0.00% } ==============================End of - } Headers==============================
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
As already said, we have the possibility to use different buffers, and it is a chance! Because each one has its advantages and drawbacks and is more suited for one application or the other. I think that for a class this fact is important to teach. Cacodylate is dangerous and must be manipulated accordingly, but in the end it must be manipulated just like any other hazardous substance! There is no specific manipulation just for cacodylate alone! In my opinion (just my opinion), cacodylate is mostly used because of inertia force ;-) In some labs, there is not way, no argumentation which can change the opinion of the boss: he always used cacodylate and always will. In my opinion (just my opinion), it would be good if we could forget about cacodylate as a "classical" buffer, especially for students. This way the next generation will be more prompt to use other buffers and perhaps keeps the cacodylate buffer just for special application, but not as part of a "classical" protocol. In the end, teaching is less about perpetuating the same things forever than about learning the basics to allow improvements (I hope this sentence is grammatically correct).
Stéphane
==============================Original Headers============================== 5, 22 -- From nizets2-at-yahoo.com Wed Jan 14 03:23:26 2009 5, 22 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0E9NQ0F027906 5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 14 Jan 2009 03:23:26 -0600 5, 22 -- Received: (qmail 45666 invoked by uid 60001); 14 Jan 2009 09:23:25 -0000 5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 22 -- s=s1024; d=yahoo.com; 5, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 22 -- b=V+D0yzi+QYhmvS7FRIWZPMCwbMurltyT/jqfNFjg79+PojhpWJ5J8fMb6atmCBM3yaMZKl6tiXP3cVhJrs6uka7nleXFyGk4FgygfWDSVLrdm0Ad1XEgNHVzGjM9msx6U219KoXYI39lTHGnFiYOyc/rRqJQHoJrcPtsEV4sBLA=; 5, 22 -- X-YMail-OSG: G.AZbNAVM1nB2XGiCf81LO69HKNHF8FBuKsLYkciMpQMnFcPqdmW9xWRTw9HyG5zaBMfjik1kP49iBfeGhTsai1ir0p2.JMEuW3cjDByKwwJWNU6pWqdQdeqwE.hjFS3G9kvPP4U.EJIf9R88s8TQa5V2j7AK3lji_iBQvoebTF0DoPo2mp2pP1D65OIDQ-- 5, 22 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Wed, 14 Jan 2009 01:23:25 PST 5, 22 -- X-Mailer: YahooMailRC/1156.77 YahooMailWebService/0.7.260.1 5, 22 -- References: {200901100431.n0A4Ve2v020832-at-ns.microscopy.com} 5, 22 -- Date: Wed, 14 Jan 2009 01:23:25 -0800 (PST) 5, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 22 -- Subject: Re: [Microscopy] RE: Cac buffer and undergrads - chancy? 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=iso-8859-1 5, 22 -- Message-ID: {819009.45662.qm-at-web110805.mail.gq1.yahoo.com} 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0E9NQ0F027906 ==============================End of - Headers==============================
I have a user who needs to use an SEM with a cryo-stage. Should be a short project needing say a single afternoon (freezing, prep, imaging). Unfortunately we do not have a cryo-stage on our SEM's.
Does anyone have or know of one for use - hopefully within a day's drive of Oxford Ohio (Think Cincinnati).
Thanks Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 4, 29 -- From edelmare-at-muohio.edu Wed Jan 14 08:39:42 2009 4, 29 -- Received: from spamfirewall.muohio.edu (walrus.mcs.muohio.edu [134.53.6.27]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0EEdgml021011 4, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 08:39:42 -0600 4, 29 -- X-ASG-Debug-ID: 1231943557-235a00b10000-Dem1zR 4, 29 -- X-Barracuda-URL: http://spamfirewall.muohio.edu:80/cgi-bin/mark.cgi 4, 29 -- Received: from mulnx23.mcs.muohio.edu (localhost [127.0.0.1]) 4, 29 -- by spamfirewall.muohio.edu (Spam Firewall) with ESMTP id 3590FEEB8536 4, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 09:32:37 -0500 (EST) 4, 29 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) by spamfirewall.muohio.edu with ESMTP id uomx3cGMe9BoB8aM for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 09:32:37 -0500 (EST) 4, 29 -- X-ASG-Whitelist: Client 4, 29 -- Received: from [192.168.1.23] ([134.53.14.105]) 4, 29 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0EEWZE8005130 4, 29 -- for {microscopy-at-Microscopy.com} ; Wed, 14 Jan 2009 09:32:37 -0500 4, 29 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 4, 29 -- To: microscopy-at-Microscopy.com 4, 29 -- Date: Wed, 14 Jan 2009 09:32:35 -0500 4, 29 -- MIME-Version: 1.0 4, 29 -- X-ASG-Orig-Subj: Looking for SEM With Cryo-stage 4, 29 -- Subject: Looking for SEM With Cryo-stage 4, 29 -- Message-ID: {496DB133.30031.2EB3BA04-at-edelmare.muohio.edu} 4, 29 -- Priority: normal 4, 29 -- X-mailer: Pegasus Mail for Windows (4.41) 4, 29 -- Content-type: text/plain; charset=US-ASCII 4, 29 -- Content-transfer-encoding: 7BIT 4, 29 -- Content-description: Mail message body 4, 29 -- X-Barracuda-Connect: mulnx23.mcs.muohio.edu[134.53.6.10] 4, 29 -- X-Barracuda-Start-Time: 1231943558 4, 29 -- X-Barracuda-Virus-Scanned: by Barracuda Spam Firewall at muohio.edu ==============================End of - Headers==============================
Phil, There are still some analog ancients in our world. I found buyers for a Durst 1200 4 X 5 but not for a floor model !38. Post the equipment on Craigslist or equivalent. Larry
oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good point, although not necessarily the case. The Art and journalism } deparments here have gone completely digital. Even strictly art } photography, the kind that uses high silver content paper and so } forth. They didn't even want a Durst enlarger. } Anybody on the list looking for a Durst Laborator enlarger in } excellent condition? With printing easel, extra lenses, etc.? } } Phil } } } Folks: } } Old photo/darkroom supplies, etc., may find a happy home in art schools. } } I have a young artist friend who is taking a photography class and the } } instructor is insisting that she start with film in order to understand } } the fundamentals of photography - particularly for B/W. Without getting } } into arguments about log/linear behavior, benefits of one over the } } other, etc., the point is that these folks may be able to utilize film } } supplies and are likely to be very appreciative of a cheap/free source. } } They may not have much use for the Type xxx film, but some of the other } } supplies could be valuable. } } Best Regards, } } Bill } } William A. Heeschen } } Microscopy, Digital Imaging } } 1897 Bldg, E-84 } } Dow Chemical } } Midland, MI 48667 } } mailto:waheeschen-at-dow.com } } } } -----Original Message----- } } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } } Sent: Monday, January 12, 2009 5:04 PM } } To: Heeschen, Bill (WA) } } Subject: [Microscopy] Old film etc } } } --- } } Greetings } } } } This is a message I never thought I would be sending. } } } } We are getting out of the film and paper photo business, and have a } } lot of 'stuff' to give away or send to the landfill. Anyone with } } questions about whether digital imaging for EM is for real, let this } } be your wake-up call. } } } } Most of what we have is old, probably not worth the cost to ship, } } unless you are desperate, or curious. } } } } We have a bunch of old Polaroid stuff - 52, 53, 55, 331, 72, 554, 572. } } I don't even know what some of this was used for, if it rings your } } bell, let me know and I can tell you more. } } } } We have lots of old photo paper. Polycontrast, Velox, etc. 8 x10 and 4 } } x5 sizes. } } } } A little 4463 and a ton of SO-163. The SO-163 is old. } } } } Bunch of misc. 35 mm rolls, B&W and color. Some 120 Tech Pan. } } } } Most of this junk has been in a freezer. Some is pretty old and past } } its expiration date, but if you are interested, let me know and we can } } try to work something out. If I don't hear anything in the next week } } or so, its outa here. } } } } Jon } } } } Jonathan Krupp } } Delta College } } 5151Pacific Ave. } } Stockton, CA 95207 } } 209-954-5284 } } jkrupp-at-deltacollege.edu }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 6, 39 -- From Larry.Ackerman-at-ucsf.edu Wed Jan 14 16:58:50 2009 6, 39 -- Received: from emfmcb02.ucsfmedicalcenter.org (emfmcb02.ucsfmedicalcenter.org [64.54.46.98]) 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0EMwn25022667 6, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2009 16:58:49 -0600 6, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with 6, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2)); 6, 39 -- Wed, 14 Jan 2009 14:58:39 -0800 6, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B 6, 39 -- X-AuditID: 403623d2-a5611bb000007fb9-ed-496e8a56654a 6, 39 -- Received: from exbhmcb01.ucsfmedicalcenter.org ( 6, 39 -- exbhmcb01.ucsfmedicalcenter.org [64.54.46.222]) by 6, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id 6, 39 -- C975430C for {Microscopy-at-microscopy.com} ; Wed, 14 Jan 2009 16:59:02 6, 39 -- -0800 (PST) 6, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by 6, 39 -- exbhmcb01.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830); 6, 39 -- Wed, 14 Jan 2009 14:58:38 -0800 6, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 6, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Wed, 14 Jan 6, 39 -- 2009 14:58:38 -0800 6, 39 -- Message-ID: {496E6E1E.8060502-at-ucsf.edu} 6, 39 -- Date: Wed, 14 Jan 2009 14:58:38 -0800 6, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 6, 39 -- Reply-to: larry.ackerman-at-ucsf.edu 6, 39 -- Organization: UCSF, NeuroAnatomy 6, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 6, 39 -- MIME-Version: 1.0 6, 39 -- To: Microscopy-at-microscopy.com 6, 39 -- Subject: Re: [Microscopy] RE: Old film etc 6, 39 -- References: {200901131505.n0DF53Nl005349-at-ns.microscopy.com} 6, 39 -- In-Reply-To: {200901131505.n0DF53Nl005349-at-ns.microscopy.com} 6, 39 -- X-OriginalArrivalTime: 14 Jan 2009 22:58:38.0895 (UTC) 6, 39 -- FILETIME=[A353B3F0:01C9769B] 6, 39 -- X-Brightmail-Tracker: AAAAAQ05Q1k= 6, 39 -- X-WSS-ID: 6570B1951OO2346768-01-01 6, 39 -- Content-Type: text/plain; 6, 39 -- charset=iso-8859-1; 6, 39 -- format=flowed 6, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: sam.telford-at-tufts.edu Name: sam telford
Organization: tufts university
Title-Subject: [Filtered] infinity dk series digital camera
Question: Does anyone have any experience with the Infinity DK Series -- Meiji makes this, I think -- digital setup (particularly the new 5.1 MP camera) for capturing images from a compound scope? I am particularly interested in reliability, ease of use, and quality of images. Are there other setups I should think about for the same price ($3400)? This would be used for publication quality documentation of blood smears (X250-X1000) or of histopathology material.
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Title-Subject: [Filtered] regarding scanning electron microscopic imaging of extracted human teeth
Question: how do i differentiate between the cementum and dentine of extracted human teeth in the micrographs obtained from scanning electron microscope? is the cementodentinal structure clearly demarcated? does cementum have any characteristic apperance when seen under scanning electro microscope?
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Question: We're currently using a CDEM detector on an FEI FIB820. We use this detector for both our Ion and SEM images. It's one of the older FEI systems and still uses Windows 3.1 with an outdated PC.
Does anyone know of a company that has a SE detector that would be compatible with our system or possibly be a standalone detector that would only need a support PC to run it?
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Hi All, We have mostly Spot cameras and Olympus cameras in our setups. Our Pathologists really like the easy to use Spot cameras. Very intuitive software and wonderful images.
-----Original Message----- X-from: sam.telford-at-tufts.edu [mailto:sam.telford-at-tufts.edu] Sent: Thursday, January 15, 2009 6:50 PM To: Joachim Siegmund
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both sam.telford-at-tufts.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: sam.telford-at-tufts.edu Name: sam telford
Organization: tufts university
Title-Subject: [Filtered] infinity dk series digital camera
Question: Does anyone have any experience with the Infinity DK Series -- Meiji makes this, I think -- digital setup (particularly the new 5.1 MP camera) for capturing images from a compound scope? I am particularly interested in reliability, ease of use, and quality of images. Are there other setups I should think about for the same price ($3400)? This would be used for publication quality documentation of blood smears (X250-X1000) or of histopathology material.
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Thu Jan 15 18:32:42 2009 6, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0G0WfMx004943 6, 11 -- for {microscopy-at-microscopy.com} ; Thu, 15 Jan 2009 18:32:42 -0600 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240800c595861916d0-at-[206.69.208.22]} 6, 11 -- Date: Thu, 15 Jan 2009 18:32:40 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: sam.telford-at-tufts.edu (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: infinity dk series digital camera 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 19, 29 -- From jsiegmund-at-7thwavelabs.com Fri Jan 16 08:50:21 2009 19, 29 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136]) 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GEoLlH024425 19, 29 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 08:50:21 -0600 19, 29 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1]) 19, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id C7A2839800A; 19, 29 -- Fri, 16 Jan 2009 08:50:20 -0600 (CST) 19, 29 -- X-AuditID: c0a80218-a7843bb000000bbe-8f-49709eac5030 19, 29 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29]) 19, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 5C363424013; 19, 29 -- Fri, 16 Jan 2009 08:50:20 -0600 (CST) 19, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 29 -- Content-class: urn:content-classes:message 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Type: text/plain; 19, 29 -- charset="us-ascii" 19, 29 -- Subject: RE: [Microscopy] viaWWW: infinity dk series digital camera 19, 29 -- Date: Fri, 16 Jan 2009 08:50:19 -0600 19, 29 -- Message-ID: {62A8156F8071C8439080D626DF8C33A658B5B4-at-wave-mail.7thwave.local} 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- Thread-Topic: [Microscopy] viaWWW: infinity dk series digital camera 19, 29 -- Thread-Index: Acl3dExx2JOtrkwNQluVccJslXOnVQAdITwg 19, 29 -- References: {200901160049.n0G0nWLF013465-at-ns.microscopy.com} 19, 29 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com} 19, 29 -- To: {sam.telford-at-tufts.edu} , {microscopy-at-microscopy.com} 19, 29 -- X-Brightmail-Tracker: AAAAAg04csgNOUNZ 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0GEoLlH024425 ==============================End of - Headers==============================
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Email: waldenzz-at-gmail.com Name: Jonathan Zhang
Organization: University of Washington
Title-Subject: [Filtered] Mount a dslr on BX51
Question:
We are users of an Olympus BX51 Microscope and trying to find a simple solution to take microscopic pictures. Since the microscope has a C mount, could we just mount a C-mount adapter and connect it to a 35 mm SLR camera (like a Nikon D40)?
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Email: qxing-at-ameslab.gov Name: Qingfeng Xing
Organization: Ames Laboratory
Title-Subject: [Filtered] X-ray Back Laue collimation system
Question: Dear colleagues:
Does anyone know the requirements for designing a collimation system for higher accuracy in back Laue X-ray diffraction?
It seems that the geometry is simple. Are there any tricks?
I am looking for a suggestion for a video camera to attach to a C mount on a disecting microscope, that can be used to make AVI or MPEG movies. Also for some simple movie making software, along the lines of iMovie but for a PC. The idea being to create Avi movies of lab protocols Thanks in advance Lloyd Williams
Sent from my iPhone
==============================Original Headers============================== 3, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 16 16:48:33 2009 3, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GMmVfW018101 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 16:48:33 -0600 3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 3, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id 3, 26 -- 8.1.336.0; Fri, 16 Jan 2009 17:48:34 -0500 3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 3, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 16 Jan 2009 3, 26 -- 17:48:50 -0500 3, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} 3, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 26 -- Date: Fri, 16 Jan 2009 17:48:23 -0500 3, 26 -- Subject: Camera and Software Suggestion 3, 26 -- Thread-Topic: Camera and Software Suggestion 3, 26 -- Thread-Index: Acl4LJljE7YkFcmIRsW1CZo7AaB86g== 3, 26 -- Message-ID: {65E3C657-D966-4305-97B1-A794B38835B4-at-genectr..hunter.cuny.edu} 3, 26 -- Accept-Language: en-US 3, 26 -- Content-Language: en-US 3, 26 -- X-MS-Has-Attach: 3, 26 -- X-MS-TNEF-Correlator: 3, 26 -- acceptlanguage: en-US 3, 26 -- Content-Type: text/plain; charset="us-ascii" 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0GMmVfW018101 ==============================End of - Headers==============================
Previous advice on this topic from Alan and Jeff was excellent. I have a couple of other caveats that you may want to consider.
One other note is that grain size measurement for these materials in most forms will be much easier with light microscopy than with SEM. Subtle topography created by the etching is often difficult to image by SEM, but readily observed by LM. You will probably not need magnification greater than about 500X unless you have something like very fine wire or thin sheet materials. We do grain size with SEM on stainless steels with very fine grains, but sample preparation and imaging are critical for accurate results.
Secondly, if the material is in a cold worked condition, it will be very difficult to see the individual grains. If you are not familiar with the techniques and structures, you could spend days working on cold-worked stainless steel thinking that your technique was bad when the actual microstructure just does not have distinctly delineated grains. Likewise, some stainless steels have a martensitic structure that may not exhibit distinct grain boundaries or the grain boundaries may be difficult to recognize without experience.
Sorry to chime in late on this topic, but thought that this might be helpful if you haven't got it all figured out already.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
} Listers - I have samples to prepare for inspection by SEM, and the client } wants to know about the grain size. We have never polished metals before. } Is there anyone who can advise me what to do, or does this take a } metallurgist? Carol Heckman, Bowling Green State University
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For a really inexpensive approach, I used a Qsee digital surveillance camera, model QSPSC, that I bought from Fry's electronics that cost about $70. I took the lens off and it was a C-mount and hooked it up to the microscope. I took the video out and put it into my video camera and recorded the image. I then could make a DVD from it. I did this for essentially the same reason that you are trying to do. The quality good enough for showing people what you are trying to do under the microscope. I've hooked this up to a DVD player and a monitor with surprising results.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: Williams-at-GENECTR.HUNTER.CUNY.EDU [mailto:Williams-at-GENECTR.HUNTER.CUNY.EDU] Sent: Friday, January 16, 2009 2:52 PM To: Walck-at-SouthBayTech.com
I am looking for a suggestion for a video camera to attach to a C mount on a disecting microscope, that can be used to make AVI or MPEG movies. Also for some simple movie making software, along the lines of iMovie but for a PC. The idea being to create Avi movies of lab protocols Thanks in advance Lloyd Williams
Sent from my iPhone
==============================Original Headers============================== 3, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 16 16:48:33 2009 3, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0GMmVfW018101 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 16:48:33 -0600 3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 3, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id 3, 26 -- 8.1.336.0; Fri, 16 Jan 2009 17:48:34 -0500 3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 3, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 16 Jan 2009 3, 26 -- 17:48:50 -0500 3, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} 3, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 26 -- Date: Fri, 16 Jan 2009 17:48:23 -0500 3, 26 -- Subject: Camera and Software Suggestion 3, 26 -- Thread-Topic: Camera and Software Suggestion 3, 26 -- Thread-Index: Acl4LJljE7YkFcmIRsW1CZo7AaB86g== 3, 26 -- Message-ID: {65E3C657-D966-4305-97B1-A794B38835B4-at-genectr..hunter.cuny.edu} 3, 26 -- Accept-Language: en-US 3, 26 -- Content-Language: en-US 3, 26 -- X-MS-Has-Attach: 3, 26 -- X-MS-TNEF-Correlator: 3, 26 -- acceptlanguage: en-US 3, 26 -- Content-Type: text/plain; charset="us-ascii" 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0GMmVfW018101 ==============================End of - Headers==============================
Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008 5:29 PM
==============================Original Headers============================== 13, 22 -- From walck-at-southbaytech.com Fri Jan 16 21:18:14 2009 13, 22 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82]) 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0H3IEqQ019522 13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 21:18:14 -0600 13, 22 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 13, 22 -- (authenticated bits=0) 13, 22 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n0H3IAmH029444; 13, 22 -- Fri, 16 Jan 2009 21:18:11 -0600 13, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 13, 22 -- To: {Microscopy-at-microscopy.com} 13, 22 -- Cc: {Williams-at-GENECTR.HUNTER.CUNY.EDU} 13, 22 -- Subject: RE: [Microscopy] Camera and Software Suggestion 13, 22 -- Date: Fri, 16 Jan 2009 19:18:30 -0800 13, 22 -- Message-ID: {8F9BA85574E9478F8728000632C5E4B8-at-dynamicbl8uno3} 13, 22 -- MIME-Version: 1.0 13, 22 -- Content-Type: text/plain; 13, 22 -- charset="us-ascii" 13, 22 -- Content-Transfer-Encoding: 7bit 13, 22 -- X-Mailer: Microsoft Office Outlook 11 13, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 13, 22 -- Thread-Index: Acl4LQY4wMt32pgzQAeGPPImp8S+DQAI7dKg 13, 22 -- In-Reply-To: {200901162251.n0GMplGZ023119-at-ns.microscopy.com} ==============================End of - Headers==============================
From nicgugliucci-at-hotmail.com Sat Jan 17 04:22:13 2009 Return-Path: {nicgugliucci-at-hotmail.com} Received: from google.com (122.2.169.154.pldt.net [122.2.169.154] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0HAMB50019014 for {microscopylistserverarchive-at-microscopy.com} ; Sat, 17 Jan 2009 04:22:12 -0600 Received: from [148.134.79.59] (HELO google.com) by gladlay.com; Sat, 17 Jan 2009 18:33:57 -0800 Message-ID: {0000000653A18321278805761} Reply-To: Allyn Losey {kimgigbank-at-yahoo.com.sg}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arnec-at-bio.umass.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arnec-at-bio.umass.edu Name: Arne
Title-Subject: [Filtered] Methods to deglycosylate fixed tissue sections
Question: I've been having trouble with an antibody directed against an epitope with a putative gycosylation site. I'm wondering if anybody can recommend a preferred method for deglycosylating (N-linked) proteins in fixed tissue sections. I'm particularly interested in enzymatic deglycosylation with PNGase F.
We still have a Boekel table top digital incubator that needs to find a home. ~ It is a brand new unit in its original shipping box.
Please contact me for pictures and specs. It will only cost you the shipping. If you want to make a donation for it to Valley Catholic HS EM Lab that's ok too.
Thank you,
Hobie
Hobie Richards Partner, and COO Technical Sales Solutions, LLC Portland, OR USA www.TechnicalSalesSolutions.com 503 781 0428
Skype Hobie-TSS
==============================Original Headers============================== 10, 19 -- From Hobie-at-technicalsalessolutions.com Sun Jan 18 20:23:47 2009 10, 19 -- Received: from host203.com (host203.com [203.194.159.243]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0J2Nh1E031231 10, 19 -- for {microscopy-at-microscopy.com} ; Sun, 18 Jan 2009 20:23:45 -0600 10, 19 -- Received: (qmail 1883 invoked by uid 503); 19 Jan 2009 02:23:40 -0000 10, 19 -- Received: from unknown (HELO ?10.0.1.195?) (Hobie-at-76.115.10.232) 10, 19 -- by host203.com with ESMTPA; 19 Jan 2009 02:23:40 -0000 10, 19 -- User-Agent: Microsoft-Entourage/12.0.0.071130 10, 19 -- Date: Sun, 18 Jan 2009 18:23:34 -0800 10, 19 -- Subject: Boekel Digital Incubator ~ Free! 10, 19 -- From: Hobie Richards {Hobie-at-technicalsalessolutions.com} 10, 19 -- To: {microscopy-at-microscopy.com} 10, 19 -- Message-ID: {C5992426.16576%Hobie-at-technicalsalessolutions.com} 10, 19 -- Thread-Topic: Boekel Digital Incubator ~ Free! 10, 19 -- Thread-Index: Acl53O1uKv/DV2wDY06DKy6vVaHJXQ== 10, 19 -- Mime-version: 1.0 10, 19 -- Content-type: text/plain; 10, 19 -- charset="US-ASCII" 10, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
The College of Microscopy, located in Westmont, IL, is offering the following electron microscopy short courses:
March 16 to 20, 2009 - Scanning Electron Microscopy
March 24 to 26, 2009 - Transmission Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Regards,
Elaine
********************************************************************* Elaine F. Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 12, 23 -- From eschumacher-at-mccrone.com Mon Jan 19 10:24:36 2009 12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0JGOZfR022953 12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 10:24:36 -0600 12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 12, 23 -- Mon, 19 Jan 2009 10:24:06 -0600 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="iso-8859-1" 12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 23 -- Subject: Short Course Announcement: SEM and TEM 12, 23 -- Date: Mon, 19 Jan 2009 10:24:05 -0600 12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A701EBB792-at-MCCRONEMSG.tmg.mccrone.com} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: Short Course Announcement: SEM and TEM 12, 23 -- Thread-Index: Acl6Ulj6k6nl7JoySoeTuoktHBriPQ== 12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 23 -- To: {microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 19 Jan 2009 16:24:06.0285 (UTC) FILETIME=[596AD3D0:01C97A52] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0JGOZfR022953 ==============================End of - Headers==============================
Scott's camera suggestion may be a good one. But rather than the runnin through another video camera, I would suggest one of the simple USB video capture devices. I got a "fancy" Diamond multimedia VC500 a couple of months ago to do similar (Already had older c-mount video cameras), and it works great. "Fancy" means it does both composite and s-video, NTSC, PAL, Etc. It cost $35 at amazon.
Since your at CUNY you might just hit some of the camera stores in The City and see what they may have in c-mount video camera's.
On 16 Jan 2009 at 22:19, walck-at-southbaytech.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } For a really inexpensive approach, I used a Qsee digital surveillance } camera, model QSPSC, that I bought from Fry's electronics that cost about } $70. I took the lens off and it was a C-mount and hooked it up to the } microscope. I took the video out and put it into my video camera and } recorded the image. I then could make a DVD from it. I did this for } essentially the same reason that you are trying to do. The quality good } enough for showing people what you are trying to do under the microscope. } I've hooked this up to a DVD player and a monitor with surprising results. } } } -Scott } } Scott D. Walck, Ph.D. } Technical Director } South Bay Technology, Inc. } 1120 Via Callejon } San Clemente, CA 92673 } } US Toll Free: 1-800-728-2233 } Tel: (949) 492-2600 } Fax: (949) 492-1499 } } www.southbaytech.com } walck-at-southbaytech.com } } -----Original Message----- } X-from: Williams-at-GENECTR.HUNTER.CUNY.EDU } [mailto:Williams-at-GENECTR.HUNTER.CUNY.EDU] } Sent: Friday, January 16, 2009 2:52 PM } To: Walck-at-SouthBayTech.com } Subject: [Microscopy] Camera and Software Suggestion } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am looking for a suggestion for a video camera to attach to a C mount on a } disecting microscope, that can be used to make AVI or MPEG movies. Also for } some simple movie making software, along the lines of iMovie but for a PC. } The idea being to create Avi movies of lab protocols Thanks in advance Lloyd } Williams } } Sent from my iPhone } } } ==============================Original Headers============================== } 3, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 16 16:48:33 2009 3, } 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu } [146.95.150.34]) } 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n0GMmVfW018101 } 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 16:48:33 } -0600 } 3, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 3, } 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server } (TLS) id 3, 26 -- 8.1.336.0; Fri, 16 Jan 2009 17:48:34 -0500 3, 26 -- } Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 3, 26 -- } Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 16 Jan 2009 } 3, 26 -- 17:48:50 -0500 3, 26 -- From: Lloyd Williams } {Williams-at-GENECTR.HUNTER.CUNY.EDU} } 3, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 26 } -- Date: Fri, 16 Jan 2009 17:48:23 -0500 3, 26 -- Subject: Camera and } Software Suggestion 3, 26 -- Thread-Topic: Camera and Software Suggestion 3, } 26 -- Thread-Index: Acl4LJljE7YkFcmIRsW1CZo7AaB86g== 3, 26 -- Message-ID: } {65E3C657-D966-4305-97B1-A794B38835B4-at-genectr..hunter.cuny.edu} } 3, 26 -- Accept-Language: en-US } 3, 26 -- Content-Language: en-US } 3, 26 -- X-MS-Has-Attach: } 3, 26 -- X-MS-TNEF-Correlator: } 3, 26 -- acceptlanguage: en-US } 3, 26 -- Content-Type: text/plain; charset="us-ascii" } 3, 26 -- MIME-Version: 1.0 } 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id n0GMmVfW018101 } ==============================End of - Headers============================== } } Internal Virus Database is out of date. } Checked by AVG - http://www.avg.com } Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008 } 5:29 PM } } } ==============================Original Headers============================== } 13, 22 -- From walck-at-southbaytech.com Fri Jan 16 21:18:14 2009 } 13, 22 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82]) } 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0H3IEqQ019522 } 13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Jan 2009 21:18:14 -0600 } 13, 22 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) } 13, 22 -- (authenticated bits=0) } 13, 22 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n0H3IAmH029444; } 13, 22 -- Fri, 16 Jan 2009 21:18:11 -0600 } 13, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} } 13, 22 -- To: {Microscopy-at-microscopy.com} } 13, 22 -- Cc: {Williams-at-GENECTR.HUNTER.CUNY.EDU} } 13, 22 -- Subject: RE: [Microscopy] Camera and Software Suggestion } 13, 22 -- Date: Fri, 16 Jan 2009 19:18:30 -0800 } 13, 22 -- Message-ID: {8F9BA85574E9478F8728000632C5E4B8-at-dynamicbl8uno3} } 13, 22 -- MIME-Version: 1.0 } 13, 22 -- Content-Type: text/plain; } 13, 22 -- charset="us-ascii" } 13, 22 -- Content-Transfer-Encoding: 7bit } 13, 22 -- X-Mailer: Microsoft Office Outlook 11 } 13, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 } 13, 22 -- Thread-Index: Acl4LQY4wMt32pgzQAeGPPImp8S+DQAI7dKg } 13, 22 -- In-Reply-To: {200901162251.n0GMplGZ023119-at-ns.microscopy.com} } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 13, 24 -- From edelmare-at-muohio.edu Mon Jan 19 13:42:28 2009 13, 24 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0JJgSxd011204 13, 24 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Jan 2009 13:42:28 -0600 13, 24 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 13, 24 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0JJgU6v018846 13, 24 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Jan 2009 14:42:30 -0500 13, 24 -- Received: from [192.168.1.23] ([134.53.14.105]) 13, 24 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0JJgRHq029772 13, 24 -- for {microscopy-at-Microscopy.com} ; Mon, 19 Jan 2009 14:42:28 -0500 13, 24 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 13, 24 -- To: microscopy-at-Microscopy.com 13, 24 -- Date: Mon, 19 Jan 2009 14:42:27 -0500 13, 24 -- MIME-Version: 1.0 13, 24 -- Subject: [Microscopy] RE: Camera and Software Suggestion 13, 24 -- Message-ID: {49749153.27429.498F08FE-at-edelmare.muohio.edu} 13, 24 -- Priority: normal 13, 24 -- In-reply-to: {200901170319.n0H3JWKH020545-at-ns.microscopy.com} 13, 24 -- References: {200901170319.n0H3JWKH020545-at-ns.microscopy.com} 13, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 13, 24 -- Content-type: text/plain; charset=US-ASCII 13, 24 -- Content-transfer-encoding: 7BIT 13, 24 -- Content-description: Mail message body 13, 24 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70 ==============================End of - Headers==============================
I am a new instructor at Delta College in Stockton, California.
I would like to make contact with anyone reading the list who has some affiliation with Delta.
If you attended Delta in the past, graduated or not, or know someone who did, could you send me some contact info. I want to make a list of folks who have left here and are now in the work force. Lots of the current students are curious about your experience, the kinds of jobs available, even specifics like hours and pay.
Even if you're not a Delta type, any info about realistic expectations for our students thinking about jobs, their plans and their future would be great. What are the skills and qualities that we need to instill in our students?
As I collect info, I'll put it together in a simple directory for us to share.
Thanks
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 42 -- From jkrupp-at-deltacollege.edu Mon Jan 19 18:13:05 2009 13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0K0CpGT005180 13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 18:13:04 -0600 13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 42 -- by localhost (Email Security Appliance) with SMTP id 5DECD1BA741 13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 23:47:43 +0000 (GMT) 13, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 13, 42 -- by mailin.deltacollege.edu (Email Security Appliance) with ESMTP id 552141689F9 13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 23:47:43 +0000 (GMT) 13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 13, 42 -- with ESMTP id 45104006 for microscopy-at-microscopy.com; Mon, 19 Jan 2009 16:12:45 -0800 13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 13, 42 -- ESMTP id KDQT7A00.UGS for {microscopy-at-microscopy.com} ; Mon, 19 13, 42 -- Jan 2009 15:57:10 -0800 13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 96FF08F74BA5 13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 16:12:45 -0800 (PST) 13, 42 -- X-Virus-Scanned: amavisd-new at 13, 42 -- X-Spam-Flag: NO 13, 42 -- X-Spam-Score: -2.499 13, 42 -- X-Spam-Level: 13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 13, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] 13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 13, 42 -- with ESMTP id Y1ixOY7peTUu for {microscopy-at-microscopy.com} ; 13, 42 -- Mon, 19 Jan 2009 16:12:41 -0800 (PST) 13, 42 -- Received: from [172.20.8.17] (unknown [172.20.8.17]) 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D59C58F74B26 13, 42 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jan 2009 16:12:41 -0800 (PST) 13, 42 -- Message-Id: {4AAB3392-6686-4E7C-B11B-2019FC8A8513-at-deltacollege.edu} 13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 42 -- To: microscopy-at-microscopy.com 13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 42 -- Content-Transfer-Encoding: 7bit 13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 13, 42 -- Subject: Calling all former Delta College students 13, 42 -- Date: Mon, 19 Jan 2009 16:12:41 -0800 13, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I'm searching for a good chemical fixation protocol for bacteria/microorganisms for an EM class that I'm developing for undergrads. Since the class hasn't been taught at my university for several years (8), it seems that most/all of the microscopy journals have been dropped from the libary. So, if you would share your protocol, I would be ever-grateful.
Many thanks, Kristen
Kristen A. Lennon, Ph.D.
Lecturer, Department of Biology
202 Compton Science Center
Frostburg State University
101 Braddock Road
Frostburg, MD 21532
301-687-4697
k.lennon-at-frostburg.edu
==============================Original Headers============================== 14, 20 -- From kamlennon-at-yahoo.com Tue Jan 20 13:35:38 2009 14, 20 -- Received: from web84003.mail.mud.yahoo.com (web84003.mail.mud.yahoo.com [68.142.206.173]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0KJZab4024990 14, 20 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2009 13:35:37 -0600 14, 20 -- Received: (qmail 15715 invoked by uid 60001); 20 Jan 2009 19:35:35 -0000 14, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 20 -- s=s1024; d=yahoo.com; 14, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 14, 20 -- b=3X+spjmYxBHstMM4Bf4f/vJFFwKPOd8u0thFOtWIh0U8jGTzdCIwfJO6e+W8cgypiQ1DZvfRtExVKg3GPrCGfYeSK9qvy2TF21oFvBBRtLwyX6GdFQc3QXykNIVWt2oSqjX/pxu6rn12DSq/AtAgSoAd0R2/Vnf85S4lco7xufI=; 14, 20 -- X-YMail-OSG: x6vDz9AVM1lQIKrJVRdey1aVkpZQbfjUZm0QZ_tNhwf59PsB2ao- 14, 20 -- Received: from [96.239.149.81] by web84003.mail.mud.yahoo.com via HTTP; Tue, 20 Jan 2009 11:35:35 PST 14, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 14, 20 -- Date: Tue, 20 Jan 2009 11:35:35 -0800 (PST) 14, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} 14, 20 -- Reply-To: kamlennon-at-yahoo.com 14, 20 -- Subject: Good fix for bacteria/microorganisms? 14, 20 -- To: microscopy-at-microscopy.com 14, 20 -- MIME-Version: 1.0 14, 20 -- Content-Type: text/plain; charset=us-ascii 14, 20 -- Message-ID: {649576.15497.qm-at-web84003.mail.mud.yahoo.com} ==============================End of - Headers==============================
Will your bacteria be negatively stained or will they be fixed inside cells, like macrophages?
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov ====== } From: {kamlennon-at-yahoo.com} } Reply-To: {kamlennon-at-yahoo.com} } Date: Tue, 20 Jan 2009 13:55:56 -0600 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] Good fix for bacteria/microorganisms? } } Hi Listers, } } I'm searching for a good chemical fixation protocol for } bacteria/microorganisms for an EM class that I'm developing for undergrads. } Since the class hasn't been taught at my university for several years (8), it } seems that most/all of the microscopy journals have been dropped from the } libary. So, if you would share your protocol, I would be ever-grateful. } } Many thanks, } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } } 301-687-4697 } } k.lennon-at-frostburg.edu
==============================Original Headers============================== 6, 27 -- From connellyps-at-nhlbi.nih.gov Tue Jan 20 14:03:53 2009 6, 27 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0KK3qpQ004833 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 20 Jan 2009 14:03:52 -0600 6, 27 -- X-IronPortListener: Outbound_SMTP 6, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) 6, 27 -- by nihxwayout.hub.nih.gov with ESMTP; 20 Jan 2009 15:03:51 -0500 6, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 6, 27 -- Tue, 20 Jan 2009 15:03:46 -0500 6, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.161]) with Microsoft Exchange Server HTTP-DAV ; 6, 27 -- Tue, 20 Jan 2009 20:03:46 +0000 6, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 6, 27 -- Date: Tue, 20 Jan 2009 15:02:57 -0500 6, 27 -- Subject: Re: [Microscopy] Good fix for bacteria/microorganisms? 6, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 6, 27 -- To: {kamlennon-at-yahoo.com} , 6, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 27 -- Message-ID: {C59B9821.2D80%connellyps-at-nhlbi.nih.gov} 6, 27 -- Thread-Topic: [Microscopy] Good fix for bacteria/microorganisms? 6, 27 -- Thread-Index: Acl7OhZYVLjdlOctEd2phwANk2Yv1A== 6, 27 -- In-Reply-To: {200901201955.n0KJtuem000581-at-ns.microscopy.com} 6, 27 -- Mime-version: 1.0 6, 27 -- Content-type: text/plain; 6, 27 -- charset="ISO-8859-1" 6, 27 -- X-OriginalArrivalTime: 20 Jan 2009 20:03:46.0826 (UTC) FILETIME=[340B86A0:01C97B3A] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0KK3qpQ004833 ==============================End of - Headers==============================
Dear listers, I am asking the following on behalf of a colleague: He wants to label bacteria in order to better see them in microscopy (both fluorescence and SEM). Apparently the morphology is not sufficient to clearly identify them. In fluorescence, labeling with DAPI worked really well, although the fluorescence bleaches pretty fast.
For SEM I thought about staining them with either Osmium, lead or uranyle, which are easy to find in a EM lab. This way there is a good chance to recognize them immediately based on the BSE contrast. If needed an EDX analysis would clear all doubts. Of course there is a risk that the labeling modifies the interaction with the substrate/material (I thought about labeling them before the binding, otherwise the support may be stained too). Labeling with antibodies is not an option because it would require to order the antibodies just for this purpose
May I ask your opinion on the question? Best regards, Stephane
==============================Original Headers============================== 5, 22 -- From nizets2-at-yahoo.com Wed Jan 21 07:03:14 2009 5, 22 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0LD3Evo030473 5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 07:03:14 -0600 5, 22 -- Received: (qmail 98891 invoked by uid 60001); 21 Jan 2009 13:03:13 -0000 5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 22 -- s=s1024; d=yahoo.com; 5, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 22 -- b=dt1hEvJb7DlA8cPy1hwvS6oTOi3Ml9/m50sQC8vcFlpJe9yrcoaTd4wiV76cgFZSytL4m+450g8/MTmwxRJGtJpXq4Yn7QdyD36uJu61sj8n61dKPTBOMs5azsFW178Nr58M68+Q0cUBzH3pmclUHmT2V/XKEHt5wgEUNqQ60i8=; 5, 22 -- X-YMail-OSG: aeKjWUIVM1kI.3GYLfakhaUPAK_nb3G3Ril86bXREmgjDvtNYm3LWrntKcPNbO7sK8.D9TOlgwjwIaZrZR0kil0ezWsBBEF9jLzAw_OgnSMX.pfSefhYqUl9Y6Dz8xnmZjA0QOLZ_xy6T7NlNr2FSsELaof4gnQE0ByMD0wg4gVD7k316lAMwlkSy4AvTw-- 5, 22 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Wed, 21 Jan 2009 05:03:13 PST 5, 22 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1 5, 22 -- References: {200901202008.n0KK8a6c013590-at-ns.microscopy.com} 5, 22 -- Date: Wed, 21 Jan 2009 05:03:13 -0800 (PST) 5, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 22 -- Subject: Labeling Bacteria for SEM 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=iso-8859-1 5, 22 -- Message-ID: {778183.98312.qm-at-web110808.mail.gq1.yahoo.com} 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0LD3Evo030473 ==============================End of - Headers==============================
We use Bacillus subtilus or E. coli in our classes, and fix with standard Karnovsky's in pH 7.2 buffer, 5 min. steps in EtOH, 30%, 50, 70, 80, 90, 95, 3x100 then CPD or embed. No Prop Ox. (Or air dry or HMDS for SEM). Note, for negative stains, we don't fix, except for what fixing the stain itself does. If you're not planning on doing negative stain in the class, I would suggest you add that. It's important for clinical work, and is a good way to get students started on the TEM. Bozzola and Dysktra both have good negative stain sections, and Maunsbach & Aufzelius has good comparisons for most TEM methods. If you don't have that last book, you want it.
Phil
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==============================Original Headers============================== 4, 25 -- From oshel1pe-at-cmich.edu Wed Jan 21 07:36:29 2009 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0LDaTAK012838 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 07:36:29 -0600 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n0LDaRY8022532 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 08:36:28 -0500 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 25 -- Wed, 21 Jan 2009 08:36:11 -0500 4, 25 -- Mime-Version: 1.0 4, 25 -- Message-Id: {f06240809c59cd40ae063-at-[141.209.160.249]} 4, 25 -- In-Reply-To: {200901201952.n0KJqc08031364-at-ns.microscopy.com} 4, 25 -- References: {200901201952.n0KJqc08031364-at-ns.microscopy.com} 4, 25 -- Date: Wed, 21 Jan 2009 08:36:10 -0500 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 25 -- Subject: Re: [Microscopy] Good fix for bacteria/microorganisms? 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 25 -- X-OriginalArrivalTime: 21 Jan 2009 13:36:11.0969 (UTC) FILETIME=[397BB310:01C97BCD] 4, 25 -- X-Canit-CHI2: 0.00 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 25 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 4, 25 -- X-CanItPRO-Stream: default 4, 25 -- X-Canit-Stats-ID: 7724980 - 95d8e9dab535 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Actually, your question is potentially loaded. What does the colleague mean by identify microorganisms by microscopy, using fluorescence and SEM. Simply visualizing by SEM is not a problem. Standard procedures should work. Striking micrographs of different m.o.s have been published over the years. I’m not sure what has been done with the projects of mine over the years - the SEM was all previous to going back to school and the EM unit was treated in a very cavalier fashion when it came to recognition. However, at least one resulted in a scanning image of a Chlamydia trachomatis inclusion body releasing and bursting being placed on the cover of a book (Sexually transmitted diseases : methods and protocols, Peeling, RW & Sparling, PF (eds.)). The key in all of this is not visualizing the m.o., it is identifying it. How do you tell it is what you see.
Specific antisera should work quite effectively for both immunofluorescent microscopy, and immunogold labeling with both negative stain TEM and for SEM. The keys are the quality of the antibody and accessibility of the target epitope. The epitopes must be on the external surface if you wish to label them for SEM or NS-TEM and they will have the greatest chance of success with immunofluorescent microscopy. I have never done this for SEM, but have for NS-TEM. It has been over 20 years ago since I did this with N. gonorrhea for negative stain processing, so I don’t have the notes handy, however, all I did was give a light fixation (0.1% Glutaraldehyde in PBS), and then react with gold labeled with an antibody to a major outer protein epitope using standard protocols, with heavy blocking!!. My memory is that the target was a porin structure. Also, this was before readily available commercial gold tagged antibodies, and so I had to make the gold and label it myself, so it was a very involved process from start to end. In this case, labeling was not wildly successful, but there was sufficient specific labeling to clearly identify the external location of the epitope. At the same time, a monoclonal Ab to C. trachomatis failed totally using the same protocol. It should be noted that the investigator never accepted that just because the monoclonal antibody worked in western blotting of transblotted gels did not mean it would work in identify targets in a ‘natural’ situation. There was never a straight answer on whether it worked in IF or EIA of whole m.o.s. The key here is that you really must stress that success is dependent upon the ability of the antibodies present to identify the target in its native state. While that may appear to be a statement of the obvious, it must be reiterated to all colleagues, collaborators, clients etc that come in the door, and frequently to ourselves so that we don’t forget it.
Saponin permeablization has worked well for pre-embedding DAB immuno electron microscopic visualization in my hands. The antibody was good, and worked well in both IF and TEM. That allowed targeting epitopes of m.o.s inside cells. At the same time, neither Triton X nor saponin permeablization allowed immunogold labeling of nucleocapsid proteins of Lassavirus. Because the antisera to the Z protein was oligopeptide monospecific (to a 9aa oligopeptide), and I could never get antisera to the whole protein from that group of collaborators, I do not know if the failure was due to an ineffective permeablization protocol or inappropriate antibody. I won’t re-state the obvious from above.
Immunofluorescence is not really the same as pre-embedding immunogold TEM, even though they may seem to be identical, and my immuno DAB protocol for labeling was essentially the same as the IF protocol used in that particular study. This is especially true if your colleague wants to try targeting internal proteins by IF. It may be a bit more difficult to access the epitopes. Penetrating the dense, hydrophobic outer structures of mycobacteria, or of mycoplasm would be a challenge. Having said that, I have used standard TEM preparative procedures to fix and embed both for sectioning, and have done immunogold labelling for internal proteins successfully with other m.o.s (although we did not realize the protein was internal when they collaborator gave me the material, and it took a little work to define what was happening when we saw the results. They were crystal clear once it was established that the epitope was on the internal face of the membrane.) Because many others have also had good success with both pre and post embedding immunogold labeling of m.o.s for internal epitopes, I would suggest that standard IF fixation and labeling would work. Again, to restate the obvious, provided the antibodies will recognize the target.
I have to restate the obvious to many people who walk in the door wanting to discuss either EM or gastroenteric virology. Sorry about repeating it, but it seems to be an occupational hazard here.
I can dig through the archives and find protocols if you want. Most of those in the lit should work, that’s where mine came from.
Paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 21 09:16:52 2009 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0LFGq16030890 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 09:16:52 -0600 11, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 11, 20 -- (authenticated bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n0LFGoPM021281; 11, 20 -- Wed, 21 Jan 2009 09:16:51 -0600 (CST) 11, 20 -- Message-ID: {49773C61.3030306-at-umanitoba.ca} 11, 20 -- Date: Wed, 21 Jan 2009 09:16:49 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 11, 20 -- MIME-Version: 1.0 11, 20 -- To: nizets2-at-yahoo.com, Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Labeling Bacteria for SEM 11, 20 -- References: {200901211305.n0LD5Q58000922-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {200901211305.n0LD5Q58000922-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
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Title-Subject: [Filtered] Quantitating virus particles in cells following TEM
Question: Hello, I have to compare the number of intracellular virus particles in infected cells using TEM. I would like to know if there is precedent for such a calculation and if so, what is an unbiased method to obtain this data.
We have been contemplating about purchasing a 8.0 mm Histo diamond knif= e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper= ience comment on how long (or how many blocks) I should expect a Histo diam= ond knife to last? I have no problem producing high quality semi-thin sect= ions with glass knives, but am hoping a diamond knife would save us some ti= me. Thank you in advance.
Hong Emory EM
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==============================Original Headers============================== 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0M5U5Rv023498 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 Jan 2009 7, 32 -- 00:29:58 -0500 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500 7, 32 -- Subject: Histo diamond knife 7, 32 -- Thread-Topic: Histo diamond knife 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ== 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- acceptlanguage: en-US 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" 7, 32 -- MIME-Version: 1.0 7, 32 -- X-emory.edu-MailScanner: Found to be clean 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu 7, 32 -- X-Spam-Status: No 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M5U5Rv023498 ==============================End of - Headers==============================
We have a histo knife, however we don't cut thicker than 500nm and not on a routine basis. I have been said that like an ultraknife, its lifetime mainly depends on what you cut. Cut butter and it will survive you. Cut nanoparticles and quantum dots and it will probably not survive your grant. Cutting soft tissue in resin does probably not significantly affect it. Personally I couldn't imagine regularly semi-thin sectionning without histoknife, it is so comfortable. Maybe I am a luxus freak :-)
Regards, Stephane
----- Original Message ---- X-from: "hyi-at-emory.edu" {hyi-at-emory.edu} To: nizets2-at-yahoo.com Sent: Thursday, January 22, 2009 6:34:37 AM
Dear All:
We have been contemplating about purchasing a 8.0 mm Histo diamond knif= e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper= ience comment on how long (or how many blocks) I should expect a Histo diam= ond knife to last? I have no problem producing high quality semi-thin sect= ions with glass knives, but am hoping a diamond knife would save us some ti= me. Thank you in advance.
Hong Emory EM
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If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
==============================Original Headers============================== 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0M5U5Rv023498 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 Jan 2009 7, 32 -- 00:29:58 -0500 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500 7, 32 -- Subject: Histo diamond knife 7, 32 -- Thread-Topic: Histo diamond knife 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ== 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- acceptlanguage: en-US 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" 7, 32 -- MIME-Version: 1.0 7, 32 -- X-emory.edu-MailScanner: Found to be clean 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu 7, 32 -- X-Spam-Status: No 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M5U5Rv023498 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 22 -- From nizets2-at-yahoo.com Thu Jan 22 02:41:24 2009 22, 22 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228]) 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0M8fO8J012980 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 02:41:24 -0600 22, 22 -- Received: (qmail 62862 invoked by uid 60001); 22 Jan 2009 08:41:23 -0000 22, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 22, 22 -- s=s1024; d=yahoo.com; 22, 22 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 22, 22 -- b=arER5TCHEvR9cLCGy/Stts2255pSPF+6wxsQAw5DCTUcTxQvNs4cibc6EFv+GwnvxHoCBYwRbkyn7DxspB5qSxEjMf5rV+boK90JSFsUlFw9b3w/LGbsrLdyy0goNk4HGLfs9mH0CixEVfqBLjPlFDmafbqbAdhEYQ8pH5avEvQ=; 22, 22 -- X-YMail-OSG: 98.knsQVM1n7xyKC0afwsbtGUfk4_PDhvvFu_Xs.c_vWUIPV.VfRtFQjDBQRBez96A4u5S.icydUpBr.GBpx.UeMFtxK1j6KAIaPJSSQcvAc17ghYIcQp3Z5bRsOW03oeiZYAKQ8arb1moDp5RosesU1lpvifrHF_FbFIsBu7f.TTn0Z4O_uC6VdNHvNjGkl27vz1kMLBxUV4c9jIzVtAgja4CoF1Qh7 22, 22 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 22 Jan 2009 00:41:23 PST 22, 22 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1 22, 22 -- References: {200901220534.n0M5Ybmx030096-at-ns.microscopy.com} 22, 22 -- Date: Thu, 22 Jan 2009 00:41:23 -0800 (PST) 22, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 22, 22 -- Subject: Re: [Microscopy] Histo diamond knife 22, 22 -- To: microscopy-at-microscopy.com 22, 22 -- MIME-Version: 1.0 22, 22 -- Content-Type: text/plain; charset=iso-8859-1 22, 22 -- Message-ID: {897590.62667.qm-at-web110805.mail.gq1.yahoo.com} 22, 22 -- Content-Transfer-Encoding: 8bit 22, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M8fO8J012980 ==============================End of - Headers==============================
I use an old diamond knife to face the block, then switch out to the histo knife. Sectioning is done at 0.33 µm.
Life span varies with usage, type of sample ( ie bone or cell culture phosphate crystals, etc are harder on the knife)
I once had a histo knife that I had cut about , say 500-600 blocks / year, and I cut big blocks often, it lasted for 7 years!
However , get glass, silicone, bone etc, and you could ruin a knife in a day.
The time saved is really huge, the quality is very good, especially if you have to section some to get to "just the right depth".
Well worth the money, and yes when I switched I did have a little bit of prideful.... I can do glass well and it's an art... thing, that goes to the wayside quick after the pleasure of working with a diamond histo knife.
So save old knives, use them to rough cut the already trimmed block, and you will get even longer life out of your knife.
Lou Ann
{ { { { { { { { {} } } } } } } } } } } } } } Lou Ann Miller, Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine University of Illinois MC=002
Room 1204 VMBSBld 2001 S Lincoln Ave Urbana, IL 61821
217-244-1567
On Jan 21, 2009, at 11:37 PM, hyi-at-emory.edu wrote:
} } } Dear All: } } We have been contemplating about purchasing a 8.0 mm Histo } diamond knif= } e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there } with exper= } ience comment on how long (or how many blocks) I should expect a } Histo diam= } ond knife to last? I have no problem producing high quality semi- } thin sect= } ions with glass knives, but am hoping a diamond knife would save us } some ti= } me. Thank you in advance. } } Hong } Emory EM }
==============================Original Headers============================== 22, 19 -- From lamiller-at-illinois.edu Thu Jan 22 07:54:05 2009 22, 19 -- Received: from expredir6.cites.uiuc.edu (expredir6.cites.uiuc.edu [128.174.5.97]) 22, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MDs4hX012649 22, 19 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 07:54:05 -0600 22, 19 -- Received: from beowulf.cvm.uiuc.edu (beowulf.cvm.uiuc.edu [130.126.16.163]) 22, 19 -- by expredir6.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id n0MDs4tP005337; 22, 19 -- Thu, 22 Jan 2009 07:54:04 -0600 (CST) 22, 19 -- Message-Id: {976ED5E6-1783-4E20-9F13-2EC599CE1B3B-at-illinois.edu} 22, 19 -- From: Lou Ann Miller {lamiller-at-illinois.edu} 22, 19 -- To: microscopy-at-microscopy.com, hyi-at-emory.edu 22, 19 -- In-Reply-To: {200901220537.n0M5bKFP002839-at-ns.microscopy.com} 22, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 22, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) 22, 19 -- Subject: Re: [Microscopy] Histo diamond knife 22, 19 -- Date: Thu, 22 Jan 2009 07:54:04 -0600 22, 19 -- References: {200901220537.n0M5bKFP002839-at-ns.microscopy.com} 22, 19 -- X-Mailer: Apple Mail (2.930.3) 22, 19 -- Content-Transfer-Encoding: 8bit 22, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0MDs4hX012649 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both allan.mitchell-at-stonebow.otago.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: allan.mitchell-at-stonebow.otago.ac.nz Name: Allan Mitchell
Organization: University of otago
Title-Subject: [Filtered] TEM of TiO2 nanotubes
Question: Hi all
I have been working with a researcher here looking at some TiO2 Nanotubes in the TEM. The researcher wants to see if his nanotubes are hollow or not. We are looking for a core of around 5 nm.
Being a biology lab we do not have any experience with such samples. I have tried dusting the fragments (provided in powder form from scrappings off a Ti plate) onto carbon/formvar grids and I have tried drying them onto a grid from a suspension in ethanol. The solvent method proved more successful at getting particles onto the film than the dusting method however in both cases they tend to be clumped.
The problem I have is the clumps must be heating up in the beam. I as soon as I start to increase the magnification to explore the edges the sample disappears and I am left with a hole in the film.
I am pushing a 100kV instrument so I suspect this may have something to with it also.
A search of the literature indicates that a lot of people are investigating TiO2 nanotubes in the TEM but nothing I have found so far talks about how the nanotubes are got onto a grid in a usable form to image. lots of info about making nanotubes.
Any thoughts or suggestions would be appreciated.
Regards
Allan
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
Hi Hong, Don't contemplate - spend the money, get the knife - make yourself happy. You will not regret it nor will you go back to using glass. Consider it a necessary luxury item - you will feel so spoiled every time you use it. Work productivity will increase tenfold. Everyone here uses them for 1um thick sections (plant material).
It's the best investment you will make in 2009;-) Beth
On Jan 22, 2009, at 12:30 AM, hyi-at-emory.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All: } } We have been contemplating about purchasing a 8.0 mm Histo } diamond knif= } e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there } with exper= } ience comment on how long (or how many blocks) I should expect a } Histo diam= } ond knife to last? I have no problem producing high quality semi- } thin sect= } ions with glass knives, but am hoping a diamond knife would save us } some ti= } me. Thank you in advance. } } Hong } Emory EM } } } This e-mail message (including any attachments) is for the sole use of } the intended recipient(s) and may contain confidential and privileged } information. If the reader of this message is not the intended } recipient, you are hereby notified that any dissemination, } distribution } or copying of this message (including any attachments) is strictly } prohibited. } } If you have received this message in error, please contact } the sender by reply e-mail message and destroy all copies of the } original message (including attachments). } } } ==============================Original } Headers============================== } 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 } 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu } [170.140.52.94]) } 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n0M5U5Rv023498 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 } -0600 } 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net } (emoryfloatdmz.cc.emory.edu [170.140.52.254]) } 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id } n0M5U0qO021756 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 } -0500 } 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net } (170.140.30.56) by } 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with } Microsoft SMTP Server } 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500 } 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net } ([10.128.11.12]) by } 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; } Thu, 22 Jan 2009 } 7, 32 -- 00:29:58 -0500 } 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500 } 7, 32 -- Subject: Histo diamond knife } 7, 32 -- Thread-Topic: Histo diamond knife } 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ== } 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- acceptlanguage: en-US } 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- X-emory.edu-MailScanner: Found to be clean } 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu } 7, 32 -- X-Spam-Status: No } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n0M5U5Rv023498 } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 20 -- From beth-at-plantbio.uga.edu Thu Jan 22 09:14:12 2009 7, 20 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MFECik010739 7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 09:14:12 -0600 7, 20 -- Received: from [128.192.26.46] ([128.192.26.46]) 7, 20 -- (authenticated user beth-at-plantbio.uga.edu) 7, 20 -- by dogwood.plantbio.uga.edu 7, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 7, 20 -- Thu, 22 Jan 2009 10:14:06 -0500 7, 20 -- Message-Id: {5A3544FC-664B-4CD8-98A4-7BBE186A69AA-at-plantbio.uga.edu} 7, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 7, 20 -- To: Yi Hong {hyi-at-emory.edu} , microscopy microscopy {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200901220530.n0M5UwAq024392-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 7, 20 -- Subject: Re: [Microscopy] Histo diamond knife 7, 20 -- Date: Thu, 22 Jan 2009 10:14:09 -0500 7, 20 -- References: {200901220530.n0M5UwAq024392-at-ns.microscopy.com} 7, 20 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Hi Hong, I agree with Beth. Do yourself a favor, get the knife! It will save you alot of time. We use diamond knives for all of our thicks. The only time we have to make glass knives is when we cut something that may have bone or hard material and then we also use glass to cut thins. If you take care of the knife as you probably do the ultra-knives, you will get alot of sections off of it. Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {beth-at-plantbio.uga.edu} To: {pekysar-at-ucdavis.edu} Sent: Thursday, January 22, 2009 7:21 AM
We purchased two for serial sectioning of fish embryos at 2 microns and are very pleased with them. For serial sections re-aligning for each fresh glass knife is out of the question. When one knife develops nicks (after many thousands of sections) and is being resharpened we use the second knife.
Geoff
hyi-at-emory.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All: } } We have been contemplating about purchasing a 8.0 mm Histo diamond knif= } e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper= } ience comment on how long (or how many blocks) I should expect a Histo diam= } ond knife to last? I have no problem producing high quality semi-thin sect= } ions with glass knives, but am hoping a diamond knife would save us some ti= } me. Thank you in advance. } } Hong } Emory EM } } } This e-mail message (including any attachments) is for the sole use of } the intended recipient(s) and may contain confidential and privileged } information. If the reader of this message is not the intended } recipient, you are hereby notified that any dissemination, distribution } or copying of this message (including any attachments) is strictly } prohibited. } } If you have received this message in error, please contact } the sender by reply e-mail message and destroy all copies of the } original message (including attachments). } } } ==============================Original Headers============================== } 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 } 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) } 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0M5U5Rv023498 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600 } 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) } 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500 } 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by } 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server } 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500 } 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by } 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 Jan 2009 } 7, 32 -- 00:29:58 -0500 } 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500 } 7, 32 -- Subject: Histo diamond knife } 7, 32 -- Thread-Topic: Histo diamond knife } 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ== } 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- acceptlanguage: en-US } 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- X-emory.edu-MailScanner: Found to be clean } 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu } 7, 32 -- X-Spam-Status: No } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0M5U5Rv023498 } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 7, 28 -- From mcauliff-at-umdnj.edu Thu Jan 22 09:59:20 2009 7, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0MFxJsL030907 7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 09:59:19 -0600 7, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 7, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 48A4EA7C2C 7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 10:59:16 -0500 (EST) 7, 28 -- Received: from umdnj.edu (unknown [10.32.15.102]) 7, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 1A32BA7C2F 7, 28 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 10:32:21 -0500 (EST) 7, 28 -- Received: from ([10.32.15.171]) 7, 28 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.4981654; 7, 28 -- Thu, 22 Jan 2009 10:30:34 -0500 7, 28 -- MIME-version: 1.0 7, 28 -- Content-transfer-encoding: 7BIT 7, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 7, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 7, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built 7, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KDV00ICJPQX82C0-at-umduwc02.umdnj.edu} for 7, 28 -- microscopy-at-microscopy.com; Thu, 22 Jan 2009 10:30:34 -0500 (EST) 7, 28 -- Message-id: {49789165.3080400-at-umdnj.edu} 7, 28 -- Date: Thu, 22 Jan 2009 10:31:49 -0500 7, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 7, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 7, 28 -- To: hyi-at-emory.edu, microscopy-at-microscopy.com 7, 28 -- Subject: Re: [Microscopy] Histo diamond knife 7, 28 -- References: {200901220531.n0M5VPPb024975-at-ns.microscopy.com} 7, 28 -- In-reply-to: {200901220531.n0M5VPPb024975-at-ns.microscopy.com} ==============================End of - Headers==============================
From stimulus-at-vodafone.net Thu Jan 22 11:41:54 2009 Return-Path: {stimulus-at-vodafone.net} Received: from inpac.com.cn ([220.194.209.44]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MHfqjI031697 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 22 Jan 2009 11:41:53 -0600 Received: from User [88.40.105.35] by inpac.com.cn with ESMTP (SMTPD-10.01) id AF5F03B8; Fri, 23 Jan 2009 01:39:43 +0800
I recommend the Histo diamond knife. At my previous job, we didn't have any complaints sectioning tissues and cell pellets at a half micron thick. By removing the chance of glass dust getting on the ultrathin diamond knife, I believe it lasted longer without nicks as well. When we used glass knives, we would face the block between glass and ultrathin diamind knife work (to remove any rare glass bits) using an old sapphire knife. I was glad to skip that step after switching to the diamond histo knife.
For one project, I sectioned a 4-5mm wide tissue section with the histo knife. I wouldn't have wanted to try that with glass!
~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu] Sent: Thursday, January 22, 2009 12:38 AM To: Sobocinski, Gregg
Dear All:
We have been contemplating about purchasing a 8.0 mm Histo diamond knif= e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper= ience comment on how long (or how many blocks) I should expect a Histo diam= ond knife to last? I have no problem producing high quality semi-thin sect= ions with glass knives, but am hoping a diamond knife would save us some ti= me. Thank you in advance.
Hong Emory EM
==============================Original Headers============================== 15, 27 -- From greggps-at-umich.edu Thu Jan 22 12:28:12 2009 15, 27 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MISCcv002582 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 12:28:12 -0600 15, 27 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by 15, 27 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Thu, 22 Jan 15, 27 -- 2009 13:28:09 -0500 15, 27 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 15, 27 -- To: "hyi-at-emory.edu" {hyi-at-emory.edu} , 15, 27 -- "microscopy-at-microscopy.com" 15, 27 -- {microscopy-at-microscopy.com} 15, 27 -- Date: Thu, 22 Jan 2009 13:28:08 -0500 15, 27 -- Subject: RE: [Microscopy] Histo diamond knife 15, 27 -- Thread-Topic: [Microscopy] Histo diamond knife 15, 27 -- Thread-Index: Acl8U4r949TpifdHQNKnQ2lv7VkOUAAadtUw 15, 27 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA3934016D4652-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} 15, 27 -- References: {200901220537.n0M5bcNu003401-at-ns.microscopy.com} 15, 27 -- In-Reply-To: {200901220537.n0M5bcNu003401-at-ns.microscopy.com} 15, 27 -- Accept-Language: en-US 15, 27 -- Content-Language: en-US 15, 27 -- X-MS-Has-Attach: 15, 27 -- X-MS-TNEF-Correlator: 15, 27 -- acceptlanguage: en-US 15, 27 -- Content-Type: text/plain; charset="us-ascii" 15, 27 -- MIME-Version: 1.0 15, 27 -- Content-Transfer-Encoding: 8bit 15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0MISCcv002582 ==============================End of - Headers==============================
I also recommend a histo diamond. I even used one to cut thin sections of intact squid tentacle tip which were several mm wide.
Does any one out there have any experience with the Pella histo diamond? I have a user who is interested in it because it comes in a 10mm edge. I've only used an 8mm Diatome.
Any comments or suggestions would be appreciated.
You can reply offline if you prefer.
Paula :-)
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
--- On Thu, 1/22/09, greggps-at-umich.edu {greggps-at-umich.edu} wrote: X-from: greggps-at-umich.edu {greggps-at-umich.edu}
I recommend the Histo diamond knife. At my previous job, we didn't have any complaints sectioning tissues and cell pellets at a half micron thick. By removing the chance of glass dust getting on the ultrathin diamond knife, I believe it lasted longer without nicks as well. When we used glass knives, we would face the block between glass and ultrathin diamind knife work (to remove any rare glass bits) using an old sapphire knife. I was glad to skip that step after switching to the diamond histo knife.
For one project, I sectioned a 4-5mm wide tissue section with the histo knife. I wouldn't have wanted to try that with glass!
~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu] Sent: Thursday, January 22, 2009 12:38 AM To: Sobocinski, Gregg
Dear All:
We have been contemplating about purchasing a 8.0 mm Histo diamond knif= e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper= ience comment on how long (or how many blocks) I should expect a Histo diam= ond knife to last? I have no problem producing high quality semi-thin sect= ions with glass knives, but am hoping a diamond knife would save us some ti= me. Thank you in advance.
Hong Emory EM
==============================Original Headers============================== 15, 27 -- From greggps-at-umich.edu Thu Jan 22 12:28:12 2009 15, 27 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MISCcv002582 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 12:28:12 -0600 15, 27 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by 15, 27 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Thu, 22 Jan 15, 27 -- 2009 13:28:09 -0500 15, 27 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 15, 27 -- To: "hyi-at-emory.edu" {hyi-at-emory.edu} , 15, 27 -- "microscopy-at-microscopy.com" 15, 27 -- {microscopy-at-microscopy.com} 15, 27 -- Date: Thu, 22 Jan 2009 13:28:08 -0500 15, 27 -- Subject: RE: [Microscopy] Histo diamond knife 15, 27 -- Thread-Topic: [Microscopy] Histo diamond knife 15, 27 -- Thread-Index: Acl8U4r949TpifdHQNKnQ2lv7VkOUAAadtUw 15, 27 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA3934016D4652-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} 15, 27 -- References: {200901220537.n0M5bcNu003401-at-ns.microscopy.com} 15, 27 -- In-Reply-To: {200901220537.n0M5bcNu003401-at-ns.microscopy.com} 15, 27 -- Accept-Language: en-US 15, 27 -- Content-Language: en-US 15, 27 -- X-MS-Has-Attach: 15, 27 -- X-MS-TNEF-Correlator: 15, 27 -- acceptlanguage: en-US 15, 27 -- Content-Type: text/plain; charset="us-ascii" 15, 27 -- MIME-Version: 1.0 15, 27 -- Content-Transfer-Encoding: 8bit 15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0MISCcv002582 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 25 -- From vapatpxs-at-yahoo.com Thu Jan 22 12:42:13 2009 35, 25 -- Received: from n26.bullet.mail.mud.yahoo.com (n26.bullet.mail.mud.yahoo.com [68.142.206.221]) 35, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0MIgDRB016839 35, 25 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 12:42:13 -0600 35, 25 -- Received: from [68.142.200.226] by n26.bullet.mail.mud.yahoo.com with NNFMP; 22 Jan 2009 18:42:12 -0000 35, 25 -- Received: from [68.142.201.244] by t7.bullet.mud.yahoo.com with NNFMP; 22 Jan 2009 18:42:12 -0000 35, 25 -- Received: from [127.0.0.1] by omp405.mail.mud.yahoo.com with NNFMP; 22 Jan 2009 18:42:12 -0000 35, 25 -- X-Yahoo-Newman-Property: ymail-3 35, 25 -- X-Yahoo-Newman-Id: 962966.68788.bm-at-omp405.mail.mud.yahoo.com 35, 25 -- Received: (qmail 40504 invoked by uid 60001); 22 Jan 2009 18:42:12 -0000 35, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 35, 25 -- s=s1024; d=yahoo.com; 35, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Message-ID; 35, 25 -- b=Nyn5x5r38szoBwO80QCw0QfYvcicKMbcv+lXHHwA8LPsA514QXgTbOJcP/TkbcVe7QMrH+E87nUfVVCSGzzjIMTL9YA0ZPFzUVQD0galZi2R0d7XP1olhvmDZp7XAQlxSy+R6kRm807TfGfWHEs93XEZBkP6uPupndSPG1YUJfw=; 35, 25 -- X-YMail-OSG: zFz2rw8VM1nNWh.LXWRMhojFaMDWDSoWIXLfqWd5.V5WjNPCefuyMwTpxTeWqU3KrU3wFcjeeVqErsliP2gOjlIwRQg.JuByP7hBoCDmg7QCk9MTubUpOaZ28dXbMXL8xVWytNcmBfx1.VkKz7Xrj5Kj5WEJKfZR1BHYv0ueKP7ye.A3hVmCV7pFRHnkdgk- 35, 25 -- Received: from [132.239.85.200] by web46115.mail.sp1.yahoo.com via HTTP; Thu, 22 Jan 2009 10:42:12 PST 35, 25 -- X-Mailer: YahooMailWebService/0.7.260.1 35, 25 -- Date: Thu, 22 Jan 2009 10:42:12 -0800 (PST) 35, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 35, 25 -- Subject: Re: [Microscopy] RE: Histo diamond knife 35, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 35, 25 -- In-Reply-To: {200901221836.n0MIaBBj014721-at-ns.microscopy.com} 35, 25 -- MIME-Version: 1.0 35, 25 -- Content-Type: text/plain; charset=us-ascii 35, 25 -- Message-ID: {282390.38574.qm-at-web46115.mail.sp1.yahoo.com} ==============================End of - Headers==============================
Dear All, since some weeks I am trying to bring a new Kimball LaB6 cathode type 423E90 up to performance. I am using the cathode in a Philips 525 SEM. My experience with the cathodes before had been that the heating current had been with the old cathodes at 11 to 13 max. The new cathode needs to have 14 to 16 max. I am using the LaB6 wehnelt cap and set the height (with a 0.5mm wehnelt aperture) at ca. 0,25mm down from the surface of the aperture disc.
Please have a look at the images: www.elektronenmikroskopie.info/lab6
With heating current at position 11 I am still getting double contours in the image (LaB6_3.jpg), which is getting better when I am using a smaller spotsize (LaB6_2.jpg). With heating current at position 14 I still have some "feeling" of double contours in the image (see LaB6_4.jpg), which disapears at smaller spotsize (LaB6_5.jpg). Best image so far is LaB6_8.jpg at 10nm spotsize and heating current position of 16 (which seems for me to be too far up the scale).
...I put the cathode in my EM420 TEM and looked at the cathode image (I am not able to do this in the SEM...). At high beam current and at heating current position ca. 12 (which is 2 steps more than on older cathodes) the flat tip of the cathode showed up nicely, resembling a "cross" and comes to an even illumination at position 14.
My question is: Is anybody out there giving me some tips if heating values might be correct? Is there a problem with the wehnelt distance? I never experienced such a behavior before... Is there a chance of cathode charging or an inappropriate value of the wehnelt voltage? I changed wehnelt values, with no better imaging.
On Jan 22, 2009, at 11:26 AM, stefan.diller-at-t-online.de wrote:
} since some weeks I am trying to bring a new Kimball LaB6 cathode type } 423E90 up to performance. } I am using the cathode in a Philips 525 SEM. } My experience with the cathodes before had been that the heating } current } had been with the old cathodes at 11 to 13 max. The new cathode } needs to } have 14 to 16 max. } I am using the LaB6 wehnelt cap and set the height (with a 0.5mm } wehnelt } aperture) at ca. 0,25mm down from the surface of the aperture disc. } } Please have a look at the images: } www.elektronenmikroskopie.info/lab6 } } With heating current at position 11 I am still getting double contours } in the image (LaB6_3.jpg), which is getting better when I am using a } smaller spotsize (LaB6_2.jpg). } With heating current at position 14 I still have some "feeling" of } double contours in the image (see LaB6_4.jpg), which disapears at } smaller spotsize (LaB6_5.jpg). } Best image so far is LaB6_8.jpg at 10nm spotsize and heating current } position of 16 (which seems for me to be too far up the scale). } } ...I put the cathode in my EM420 TEM and looked at the cathode image } (I } am not able to do this in the SEM...). } At high beam current and at heating current position ca. 12 (which } is 2 } steps more than on older cathodes) the flat tip of the cathode } showed up } nicely, resembling a "cross" and comes to an even illumination at } position 14. } } My question is: } Is anybody out there giving me some tips if heating values might be } correct? Is there a problem with the wehnelt distance? } I never experienced such a behavior before... } Is there a chance of cathode charging or an inappropriate value of the } wehnelt voltage? I changed wehnelt values, with no better imaging.
Dear Stefan, If you are comparing the Kimball LaB6 to another brand, then it is not too surprising that the heating currents are different. Kimball mounts the LaB6 crystal in a cup, which is heated and transfers the heat to the filament; whereas, some other brands allow the current to go through the LaB6 directly. There may well be differences in heat transfer that require a higher current for the Kimball. In any event, it is best to operate with the filament saturated (assuming that you are not interested in operating in tip mode, where only the flat of the filament emits electrons). If the higher current required makes the life of the tip too short, then you may want to use a different brand of tip; however, our experience with Kimball LaB6 tips has been quite good. Our experience may not be too good a guide, since we have only TEMs, but I think the requirements for good performance are pretty much the same--high brightness and good coherence. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Jan 22 13:54:12 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MJsBV2014495 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 13:54:11 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 2E911329E5E 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:11 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id CEC88328F6E 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:09 -0800 (PST) 6, 22 -- Message-Id: {6705092A-D812-4998-B81C-0596D24E8071-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] LaB6 problems 6, 22 -- Date: Thu, 22 Jan 2009 11:54:09 -0800 6, 22 -- References: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Hi Hong, We have four large histoknives, usually one for trimming, two for everyday use, and one in perfect shape for when one of the others has to go away for resharpening. They are used to cut sections up to 2 microns thick, of quite large blockfaces - up to 3 mm across. They get resharpened at least once a year. Like Stephane, I'll never go back to routine glass knife use, the diamond knives save so much time. We only go back to glass for training and if the tissue might damage the diamond (chunks of rock in soil around roots, for example...).
cheers, Roseamry
On 22/01/09 7:47 PM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Hong! } } We have a histo knife, however we don't cut thicker than 500nm and not on a } routine basis. } I have been said that like an ultraknife, its lifetime mainly depends on what } you cut. Cut butter and it will survive you. } Cut nanoparticles and quantum dots and it will probably not survive your } grant. Cutting soft tissue in resin does probably not significantly affect it. } Personally I couldn't imagine regularly semi-thin sectionning without } histoknife, it is so comfortable. Maybe I am a luxus freak :-) } } Regards, } Stephane } } } } ----- Original Message ---- } X-from: "hyi-at-emory.edu" {hyi-at-emory.edu} } To: nizets2-at-yahoo.com } Sent: Thursday, January 22, 2009 6:34:37 AM } Subject: [Microscopy] Histo diamond knife } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All: } } We have been contemplating about purchasing a 8.0 mm Histo diamond knif= } e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there with exper= } ience comment on how long (or how many blocks) I should expect a Histo diam= } ond knife to last? I have no problem producing high quality semi-thin sect= } ions with glass knives, but am hoping a diamond knife would save us some ti= } me. Thank you in advance. } } Hong } Emory EM } } } This e-mail message (including any attachments) is for the sole use of } the intended recipient(s) and may contain confidential and privileged } information. If the reader of this message is not the intended } recipient, you are hereby notified that any dissemination, distribution } or copying of this message (including any attachments) is strictly } prohibited. } } If you have received this message in error, please contact } the sender by reply e-mail message and destroy all copies of the } original message (including attachments). } } } ==============================Original Headers============================== } 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 } 7, 32 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) } 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n0M5U5Rv023498 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 23:30:05 -0600 } 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net } (emoryfloatdmz.cc.emory.edu [170.140.52.254]) } 7, 32 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500 } 7, 32 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by } 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP } Server } 7, 32 -- (TLS) id 8.1.336.0; Thu, 22 Jan 2009 00:29:53 -0500 } 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by } 7, 32 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Thu, 22 } Jan 2009 } 7, 32 -- 00:29:58 -0500 } 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Date: Thu, 22 Jan 2009 00:29:57 -0500 } 7, 32 -- Subject: Histo diamond knife } 7, 32 -- Thread-Topic: Histo diamond knife } 7, 32 -- Thread-Index: Acl8UnZCC6wF823SlkShPDngGXzfdQ== } 7, 32 -- Message-ID: {C59D6E85.13E5%hyi-at-emory.edu} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- acceptlanguage: en-US } 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- X-emory.edu-MailScanner: Found to be clean } 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu } 7, 32 -- X-Spam-Status: No } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n0M5U5Rv023498 } ==============================End of - Headers============================== } } } } } } } ==============================Original Headers============================== } 22, 22 -- From nizets2-at-yahoo.com Thu Jan 22 02:41:24 2009 } 22, 22 -- Received: from web110805.mail.gq1.yahoo.com } (web110805.mail.gq1.yahoo.com [67.195.13.228]) } 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } n0M8fO8J012980 } 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 02:41:24 -0600 } 22, 22 -- Received: (qmail 62862 invoked by uid 60001); 22 Jan 2009 08:41:23 } -0000 } 22, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 22, 22 -- s=s1024; d=yahoo.com; } 22, 22 -- } h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:C } ontent-Type:Content-Transfer-Encoding:Message-ID; } 22, 22 -- } b=arER5TCHEvR9cLCGy/Stts2255pSPF+6wxsQAw5DCTUcTxQvNs4cibc6EFv+GwnvxHoCBYwRbkyn } 7DxspB5qSxEjMf5rV+boK90JSFsUlFw9b3w/LGbsrLdyy0goNk4HGLfs9mH0CixEVfqBLjPlFDmafb } qbAdhEYQ8pH5avEvQ=; } 22, 22 -- X-YMail-OSG: } 98.knsQVM1n7xyKC0afwsbtGUfk4_PDhvvFu_Xs.c_vWUIPV.VfRtFQjDBQRBez96A4u5S.icydUpB } r.GBpx.UeMFtxK1j6KAIaPJSSQcvAc17ghYIcQp3Z5bRsOW03oeiZYAKQ8arb1moDp5RosesU1lpvi } frHF_FbFIsBu7f.TTn0Z4O_uC6VdNHvNjGkl27vz1kMLBxUV4c9jIzVtAgja4CoF1Qh7 } 22, 22 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via } HTTP; Thu, 22 Jan 2009 00:41:23 PST } 22, 22 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1 } 22, 22 -- References: {200901220534.n0M5Ybmx030096-at-ns.microscopy.com} } 22, 22 -- Date: Thu, 22 Jan 2009 00:41:23 -0800 (PST) } 22, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 22, 22 -- Subject: Re: [Microscopy] Histo diamond knife } 22, 22 -- To: microscopy-at-microscopy.com } 22, 22 -- MIME-Version: 1.0 } 22, 22 -- Content-Type: text/plain; charset=iso-8859-1 } 22, 22 -- Message-ID: {897590.62667.qm-at-web110805.mail.gq1.yahoo.com} } 22, 22 -- Content-Transfer-Encoding: 8bit } 22, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n0M8fO8J012980 } ==============================End of - Headers==============================
On Jan 22, 2009, at 6:11 AM, allan.mitchell-at-stonebow.otago.ac.nz wrote:
} I have been working with a researcher here looking at some TiO2 } Nanotubes in the TEM. The researcher wants to see if his nanotubes } are hollow or not. We are looking for a core of around 5 nm. } } Being a biology lab we do not have any experience with such samples. } I have tried dusting the fragments (provided in powder form from } scrappings off a Ti plate) onto carbon/formvar grids and I have } tried drying them onto a grid from a suspension in ethanol. The } solvent method proved more successful at getting particles onto the } film than the dusting method however in both cases they tend to be } clumped. } } The problem I have is the clumps must be heating up in the beam. I } as soon as I start to increase the magnification to explore the edges } the sample disappears and I am left with a hole in the film. } } I am pushing a 100kV instrument so I suspect this may have something } to with it also. } } A search of the literature indicates that a lot of people are } investigating TiO2 nanotubes in the TEM but nothing I have found so } far talks about how the nanotubes are got onto a grid in a usable } form to image. lots of info about making nanotubes. } } Any thoughts or suggestions would be appreciated.
Dear Allan, I have not had experience with TiO2 nanotubes, but I do have a couple of suggestions. Since the EtOH suspension method seems to work better than dusting, but still results in clumping, you might try a more volatile solvent, or applying the suspension in a higher-temperature environment, such as a warm room. Faster evaporation of the solvent should reduce clumping. I suspect that it is charging of the nanotubes, rather than heating, that is causing problems. Especially if you are using a slot grid, charge buildup on the film can cause it to break. I suggest evaporating a layer of carbon onto the grid before trying to look it, and be sure that the objective aperture is inserted--backscattering from the aperture can neutralize some of the built-up charge. Good luck. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 23 -- From tivol-at-caltech.edu Thu Jan 22 17:00:31 2009 6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MN0VAe022170 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 17:00:31 -0600 6, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id E11E966E20AC; 6, 23 -- Thu, 22 Jan 2009 15:00:30 -0800 (PST) 6, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 23 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 58D4166E334F; 6, 23 -- Thu, 22 Jan 2009 15:00:29 -0800 (PST) 6, 23 -- Cc: microscopy-at-microscopy.com 6, 23 -- Message-Id: {BDB8ECB8-AA2C-4F8F-930B-D813C1CD8878-at-caltech.edu} 6, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz 6, 23 -- In-Reply-To: {200901221411.n0MEBaVO026977-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 23 -- Subject: Re: [Microscopy] viaWWW: TEM of TiO2 nanotubes 6, 23 -- Date: Thu, 22 Jan 2009 15:00:28 -0800 6, 23 -- References: {200901221411.n0MEBaVO026977-at-ns.microscopy.com} 6, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Stefan: Bill is correct. It's been years since I ran a Kimball LaB6 in an SEM (or any other thermionic tip having gone the FE route) but Kimball tips need higher saturation currents than other manufacturers. I don't remember if I even had numbers on my saturation knob, but as Bill says, run it up to saturation and use it there.
tivol-at-caltech.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Jan 22, 2009, at 11:26 AM, stefan.diller-at-t-online.de wrote: } } } } since some weeks I am trying to bring a new Kimball LaB6 cathode type } } 423E90 up to performance. } } I am using the cathode in a Philips 525 SEM. } } My experience with the cathodes before had been that the heating } } current } } had been with the old cathodes at 11 to 13 max. The new cathode } } needs to } } have 14 to 16 max. } } I am using the LaB6 wehnelt cap and set the height (with a 0.5mm } } wehnelt } } aperture) at ca. 0,25mm down from the surface of the aperture disc. } } } } Please have a look at the images: } } www.elektronenmikroskopie.info/lab6 } } } } With heating current at position 11 I am still getting double contours } } in the image (LaB6_3.jpg), which is getting better when I am using a } } smaller spotsize (LaB6_2.jpg). } } With heating current at position 14 I still have some "feeling" of } } double contours in the image (see LaB6_4.jpg), which disapears at } } smaller spotsize (LaB6_5.jpg). } } Best image so far is LaB6_8.jpg at 10nm spotsize and heating current } } position of 16 (which seems for me to be too far up the scale). } } } } ...I put the cathode in my EM420 TEM and looked at the cathode image } } (I } } am not able to do this in the SEM...). } } At high beam current and at heating current position ca. 12 (which } } is 2 } } steps more than on older cathodes) the flat tip of the cathode } } showed up } } nicely, resembling a "cross" and comes to an even illumination at } } position 14. } } } } My question is: } } Is anybody out there giving me some tips if heating values might be } } correct? Is there a problem with the wehnelt distance? } } I never experienced such a behavior before... } } Is there a chance of cathode charging or an inappropriate value of the } } wehnelt voltage? I changed wehnelt values, with no better imaging. } } } } } Dear Stefan, } If you are comparing the Kimball LaB6 to another brand, then it is } not too surprising that the heating currents are different. Kimball } mounts the LaB6 crystal in a cup, which is heated and transfers the } heat to the filament; whereas, some other brands allow the current to } go through the LaB6 directly. There may well be differences in heat } transfer that require a higher current for the Kimball. In any event, } it is best to operate with the filament saturated (assuming that you } are not interested in operating in tip mode, where only the flat of } the filament emits electrons). If the higher current required makes } the life of the tip too short, then you may want to use a different } brand of tip; however, our experience with Kimball LaB6 tips has been } quite good. Our experience may not be too good a guide, since we have } only TEMs, but I think the requirements for good performance are } pretty much the same--high brightness and good coherence. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 6, 22 -- From tivol-at-caltech.edu Thu Jan 22 13:54:12 2009 } 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MJsBV2014495 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 13:54:11 -0600 } 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) } 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 2E911329E5E } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:11 -0800 (PST) } 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new } 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) } 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id CEC88328F6E } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 11:54:09 -0800 (PST) } 6, 22 -- Message-Id: {6705092A-D812-4998-B81C-0596D24E8071-at-caltech.edu} } 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- In-Reply-To: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com} } 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 6, 22 -- Content-Transfer-Encoding: 7bit } 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 6, 22 -- Subject: Re: [Microscopy] LaB6 problems } 6, 22 -- Date: Thu, 22 Jan 2009 11:54:09 -0800 } 6, 22 -- References: {200901221926.n0MJQ7kp032688-at-ns.microscopy.com} } 6, 22 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers============================== } } }
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==============================Original Headers============================== 4, 22 -- From r-holdford-at-ti.com Thu Jan 22 18:03:05 2009 4, 22 -- Received: from comal.ext.ti.com (comal.ext.ti.com [198.47.26.152]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0N034TR004877 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 18:03:05 -0600 4, 22 -- Received: from dlep33.itg.ti.com ([157.170.170.112]) 4, 22 -- by comal.ext.ti.com (8.13.7/8.13.7) with ESMTP id n0N02vp3015082; 4, 22 -- Thu, 22 Jan 2009 18:03:02 -0600 4, 22 -- Received: from [156.117.248.174] (localhost [127.0.0.1]) 4, 22 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id n0N02vIS001360; 4, 22 -- Thu, 22 Jan 2009 18:02:57 -0600 (CST) 4, 22 -- Message-ID: {49790931.4090402-at-ti.com} 4, 22 -- Date: Thu, 22 Jan 2009 18:02:57 -0600 4, 22 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 22 -- Organization: SC Packaging Development -- FA Development 4, 22 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 4, 22 -- MIME-Version: 1.0 4, 22 -- To: stefan.diller-at-t-online.de, MSA Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] Re: LaB6 problems 4, 22 -- References: {200901221954.n0MJsOfh014754-at-ns.microscopy.com} 4, 22 -- In-Reply-To: {200901221954.n0MJsOfh014754-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The method below was sent to the list as a method to solve the same type of problem in the context of SEM, but it should also work for TEM samples on a carbon film. Echoes of the ethernet - old friends come back to visit.
Dale
Sent by } Henk Colijn } colijn.1-at-osu.edu } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] -- } } Oldrich Benada wrote: } ===================================================== } I need some advice. I was asked to do some analysis of silica particles } (size distribution) for chemist in our institute. Particle size should be in } the range of 3 to 6 um. I do not have any experiences with such sample. } Could someone give me a tip how to prepare sample for TEM (or SEM)? } ====================================================== } } The problem is that those pesky silica particles don't know that they are } supposed to separate and stay away from each other when dispersed in a } liquid followed by a droplet of this liquid suspension being placed on a } solid surface. They tend to agglomerate very quickly leading to a difficult- } to-analyze situation, especially using automated means of analysis. You are } correct in that the size range expected could be on the order of 3-6 nm. } } This is the ideal application for the camphor/naphthalene method which I } described several years ago. Credit for the technique, or at least the one } who taught it to me was an innovative microscopist then working at the } DuPont Experimental Station in Wilmington, DE by the name of Robert P. } Schatz, in about 1968, now deceased. Take a 60% camphor/40% naphthalene } mixture and heat it to twenty or so degrees above room temperature on a hot } plate in a small beaker or flask, the two organics are miscible in each } other and this is the eutectic composition. } } Once a clear liquid, add a small amount of the silica (not more than 0.1%), } which disperses quite readily. Then, using a pipette, take out some liquid } and put a drop onto a carbon coated glass slide, at which time the drop is } instantly frozen solid (it is at room temperature). Put the slide into your } vacuum evaporator to pump out all night, and the "magic" is that the solid } eutectic sublimes at room temperature at a rate that by morning, it is } completely gone, leaving the silica particles uniformly dispersed on the } carbon film! } } The rest is obvious. You can pick this up on a grid, as is, or in order to } bring out more contrast, Pt/C shadow, probably using an angle not more than } 30 degrees. You can float the "replica" off of the slide directly onto a } grid and viola! you have particles completely dispersed, virtually no } doublets or triplets, and a field quite amenable for automated image } analysis (as a bonus). } } One important further suggestion: Some times these silica particles tend to } fuse together as little "chains". If you suspect this is happening, be sure } to take the micrographs as stereo pairs because you can in fact capture this } three dimensional spatial information. } } Disclaimer: We do not sell either the camphor or naphthalene so have no } vested interest in whether people use this method or not. It is just a } really neat method for the preparation of fine particle samples in this size } range. We are obviously set up to use this method as a service for others, } however. } } Chuck
tivol-at-caltech.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Jan 22, 2009, at 6:11 AM, allan.mitchell-at-stonebow.otago.ac.nz wrote: } } } I have been working with a researcher here looking at some TiO2 } } Nanotubes in the TEM. The researcher wants to see if his nanotubes } } are hollow or not. We are looking for a core of around 5 nm. } } } } Being a biology lab we do not have any experience with such samples. } } I have tried dusting the fragments (provided in powder form from } } scrappings off a Ti plate) onto carbon/formvar grids and I have } } tried drying them onto a grid from a suspension in ethanol. The } } solvent method proved more successful at getting particles onto the } } film than the dusting method however in both cases they tend to be } } clumped. } } } } The problem I have is the clumps must be heating up in the beam. I } } as soon as I start to increase the magnification to explore the edges } } the sample disappears and I am left with a hole in the film. } } } } I am pushing a 100kV instrument so I suspect this may have something } } to with it also. } } } } A search of the literature indicates that a lot of people are } } investigating TiO2 nanotubes in the TEM but nothing I have found so } } far talks about how the nanotubes are got onto a grid in a usable } } form to image. lots of info about making nanotubes. } } } } Any thoughts or suggestions would be appreciated. } } } Dear Allan, } I have not had experience with TiO2 nanotubes, but I do have a couple } of suggestions. Since the EtOH suspension method seems to work better } than dusting, but still results in clumping, you might try a more } volatile solvent, or applying the suspension in a higher-temperature } environment, such as a warm room. Faster evaporation of the solvent } should reduce clumping. I suspect that it is charging of the } nanotubes, rather than heating, that is causing problems. Especially } if you are using a slot grid, charge buildup on the film can cause it } to break. I suggest evaporating a layer of carbon onto the grid } before trying to look it, and be sure that the objective aperture is } inserted--backscattering from the aperture can neutralize some of the } built-up charge. Good luck. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 6, 23 -- From tivol-at-caltech.edu Thu Jan 22 17:00:31 2009 } 6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) } 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MN0VAe022170 } 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 17:00:31 -0600 } 6, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) } 6, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id E11E966E20AC; } 6, 23 -- Thu, 22 Jan 2009 15:00:30 -0800 (PST) } 6, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new } 6, 23 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) } 6, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 58D4166E334F; } 6, 23 -- Thu, 22 Jan 2009 15:00:29 -0800 (PST) } 6, 23 -- Cc: microscopy-at-microscopy.com } 6, 23 -- Message-Id: {BDB8ECB8-AA2C-4F8F-930B-D813C1CD8878-at-caltech.edu} } 6, 23 -- From: Bill Tivol {tivol-at-caltech.edu} } 6, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz } 6, 23 -- In-Reply-To: {200901221411.n0MEBaVO026977-at-ns.microscopy.com} } 6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 6, 23 -- Content-Transfer-Encoding: 7bit } 6, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 6, 23 -- Subject: Re: [Microscopy] viaWWW: TEM of TiO2 nanotubes } 6, 23 -- Date: Thu, 22 Jan 2009 15:00:28 -0800 } 6, 23 -- References: {200901221411.n0MEBaVO026977-at-ns.microscopy.com} } 6, 23 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 20 -- From dac-at-research.umass.edu Thu Jan 22 18:51:17 2009 7, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0N0pHJ5019356 7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 18:51:17 -0600 7, 20 -- Received: from [192.168.1.103] (static.unknown.charter.com [96.39.6.64] (may be forged)) 7, 20 -- (authenticated bits=0) 7, 20 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0N0pGdN004141 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 19:51:16 -0500 7, 20 -- Message-ID: {497914BD.4020505-at-research.umass.edu} 7, 20 -- Date: Thu, 22 Jan 2009 19:52:13 -0500 7, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 7, 20 -- MIME-Version: 1.0 7, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 7, 20 -- Subject: Re: [Microscopy] Re: viaWWW: TEM of TiO2 nanotubes 7, 20 -- References: {200901222307.n0MN7LOl030357-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200901222307.n0MN7LOl030357-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I second all the suggestions of Bill, which you really should try. I would like to add some suggestions. Take carbon-coated grids (without formvar, or dissolve formvar from your grids). Heat the grids themselves and deposit a drop of your suspension (in methanol f.ex) on the grid, the solvent will evaporate immediately. Now one possility is that the nanotubes are already clumped in solution. In this case perhaps you can ultrasonicate them. I don't know if the treatment may be deleterious to the structure, but if try you'll know. Perhaps you start with agglomerated nanotubes. If you just want to see the internal core, I suppose you don't mind about breaking the tubes. In this case you may want to crush them in a mill or in a mortar to obtain a fine powder. And, last but not least: just sort the powder yourself! By centrifuging the suspension, you'll pellet the bigger agglomerates and keep the finest powder in suspension. You can do it in ethanol. You'll just have to adjust the parameters to reach the optimal sorting for your needs. I suspect that centrifuging at 200g for 30 min would keep particulates of approx. 500 nm in suspension.
Best regards,
Stephane
PS: not sure about it, but in case post-coating with carbon does not suffice, I wouldn't be surprised if thin coating with gold would prevent charging without much disturbing the primary electrons.
----- Original Message ---- X-from: "tivol-at-caltech.edu" {tivol-at-caltech.edu} To: nizets2-at-yahoo.com Sent: Friday, January 23, 2009 12:06:18 AM
On Jan 22, 2009, at 6:11 AM, allan.mitchell-at-stonebow.otago.ac.nz wrote:
} I have been working with a researcher here looking at some TiO2 } Nanotubes in the TEM. The researcher wants to see if his nanotubes } are hollow or not. We are looking for a core of around 5 nm. } } Being a biology lab we do not have any experience with such samples. } I have tried dusting the fragments (provided in powder form from } scrappings off a Ti plate) onto carbon/formvar grids and I have } tried drying them onto a grid from a suspension in ethanol. The } solvent method proved more successful at getting particles onto the } film than the dusting method however in both cases they tend to be } clumped. } } The problem I have is the clumps must be heating up in the beam. I } as soon as I start to increase the magnification to explore the edges } the sample disappears and I am left with a hole in the film. } } I am pushing a 100kV instrument so I suspect this may have something } to with it also. } } A search of the literature indicates that a lot of people are } investigating TiO2 nanotubes in the TEM but nothing I have found so } far talks about how the nanotubes are got onto a grid in a usable } form to image. lots of info about making nanotubes. } } Any thoughts or suggestions would be appreciated.
Dear Allan, I have not had experience with TiO2 nanotubes, but I do have a couple of suggestions. Since the EtOH suspension method seems to work better than dusting, but still results in clumping, you might try a more volatile solvent, or applying the suspension in a higher-temperature environment, such as a warm room. Faster evaporation of the solvent should reduce clumping. I suspect that it is charging of the nanotubes, rather than heating, that is causing problems. Especially if you are using a slot grid, charge buildup on the film can cause it to break. I suggest evaporating a layer of carbon onto the grid before trying to look it, and be sure that the objective aperture is inserted--backscattering from the aperture can neutralize some of the built-up charge. Good luck. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 23 -- From tivol-at-caltech.edu Thu Jan 22 17:00:31 2009 6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0MN0VAe022170 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 17:00:31 -0600 6, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id E11E966E20AC; 6, 23 -- Thu, 22 Jan 2009 15:00:30 -0800 (PST) 6, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 23 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 58D4166E334F; 6, 23 -- Thu, 22 Jan 2009 15:00:29 -0800 (PST) 6, 23 -- Cc: microscopy-at-microscopy.com 6, 23 -- Message-Id: {BDB8ECB8-AA2C-4F8F-930B-D813C1CD8878-at-caltech.edu} 6, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz 6, 23 -- In-Reply-To: {200901221411.n0MEBaVO026977-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 23 -- Subject: Re: [Microscopy] viaWWW: TEM of TiO2 nanotubes 6, 23 -- Date: Thu, 22 Jan 2009 15:00:28 -0800 6, 23 -- References: {200901221411.n0MEBaVO026977-at-ns.microscopy.com} 6, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 23, 23 -- From nizets2-at-yahoo.com Fri Jan 23 02:51:14 2009 23, 23 -- Received: from web110803.mail.gq1.yahoo.com (web110803.mail.gq1.yahoo.com [67.195.13.226]) 23, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0N8pDoE015170 23, 23 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 02:51:14 -0600 23, 23 -- Received: (qmail 1088 invoked by uid 60001); 23 Jan 2009 08:51:13 -0000 23, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 23 -- s=s1024; d=yahoo.com; 23, 23 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 23, 23 -- b=d7Yg7HjoJDFJDDnuPeBZ0EWxSIekKoXRF7kvph17C9cxKji57Ol7UeNO5wX0lNkZw/Q8hsXQLwT1vheBcJ/0euB76RKWWqPM12zN8CVuOwroq4r47J583aBlRuBZtW1CoQSL+6hQINvM+Z/3HcKfbLnCSk+rjWOGEtCsd8EZDlg=; 23, 23 -- X-YMail-OSG: S2IVPL4VM1mcckkpPaikPtjfhOR6JNgirF8DNcROOHE2Hzwgnj.WWTwApHKOtDAmGgMU3vNvlEsVsazHnEmIWSiCes8fFm6KdsycqOlRcPxCCdPqvjfRN5QQ9_F.C6FIfg555X52zMuBuLg3bH9KiJDoK9uwIhjb39VzgvOR.M8ZNJXJ1yLYMMPhmZMytHq8vy3V3CXIjo0VO2e_OaRLNovA.LQ6I8ql 23, 23 -- Received: from [80.122.101.100] by web110803.mail.gq1.yahoo.com via HTTP; Fri, 23 Jan 2009 00:51:13 PST 23, 23 -- X-Mailer: YahooMailRC/1156.82 YahooMailWebService/0.7.260.1 23, 23 -- References: {200901222306.n0MN6IeD028777-at-ns.microscopy.com} 23, 23 -- Date: Fri, 23 Jan 2009 00:51:13 -0800 (PST) 23, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com} 23, 23 -- Subject: Re: [Microscopy] Re: viaWWW: TEM of TiO2 nanotubes 23, 23 -- To: allan.mitchell-at-stonebow.otago.ac.nz 23, 23 -- Cc: microscopy-at-microscopy.com 23, 23 -- MIME-Version: 1.0 23, 23 -- Content-Type: text/plain; charset=iso-8859-1 23, 23 -- Message-ID: {461322.852.qm-at-web110803.mail.gq1.yahoo.com} 23, 23 -- Content-Transfer-Encoding: 8bit 23, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0N8pDoE015170 ==============================End of - Headers==============================
We are pleased to invite you to submit an abstract to session HE1 at the 14th International Clay Conference scheduled in June 14th-20th 2009 in Castellaneta Marina, near Bari, in Southeast Italy = (http://www.14icc.org/ ).
The abstract deadline has been extended to January 31.
Please note that contributions to 14th International Clay Conference may = be submitted for publication in a special issue of Applied Clay Science. = They will pass the usual peer review process and the issue is expected to be published before the end of 2010.
With ours best wishes for the New Year, the convenors Elena Belluso (elena.belluso-at-unito.it) and Mickey Gunter(mgunter-at-uidaho.edu)
Monitoring, identification, and quantification of asbestos are essential aspects of dealing with these minerals. These investigations are very important to the regulatory community because special precautions must = be taken when asbestos is found in significant amounts.
Different techniques of monitoring and analysis are necessary depending = on where the asbestos occurs: air, water, soils, rocks, biological = materials, asbestos-containing materials and their transformation products.
Besides, for asbestos use in health-based studies it is important to = apply several complementary analytical methods.
This session will: 1) present the state of the art in monitoring and techniques actually considered the most suitable for the different = asbestos containing materials; 2) compare various investigation protocols; 3) exchange information about the advances in this topic; and 4) develop interdisciplinary collaborations.
Convenors: Elena Belluso (elena.belluso-at-unito.it) and Mickey Gunter (mgunter-at-uidaho.edu)
----------------------------------------------------------------------------------- Prof. Elena BELLUSO - Ph.D. Mineralogy and Crystallography Dipartimento di Scienze Mineralogiche e Petrologiche Universita' degli Studi di Torino Via Valperga Caluso, 35 I-10125 TORINO - ITALIA tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 e-mail: elena.belluso-at-unito.it http://www.dsmp.unito.it ----------------------------------------------------------------------------------- "I've... seen things you people wouldn't believe. Attack ships on fire off the shoulder of Orion. I watched C-beams... glitter in the dark near the Tanhauser Gate. All those... moments will be lost... in time..., like... tears... in... rain." Blade Runner
==============================Original Headers============================== 18, 29 -- From elena.belluso-at-unito.it Fri Jan 23 03:58:54 2009 18, 29 -- Received: from mail-out.unito.it (opterone.unito.it [130.192.119.88]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0N9wrhx030275 18, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 03:58:54 -0600 18, 29 -- Received: (from root-at-localhost) 18, 29 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) id n0N9wrKt026227 18, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 10:58:53 +0100 18, 29 -- Received: from mail.unito.it (giove.unito.it [130.192.119.45]) 18, 29 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) with SMTP id n0N9wpOK026202 18, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 10:58:51 +0100 18, 29 -- X-Icontrol: Sent by Inrete Icontrol 18, 29 -- Received: from 130.192.111.68 18, 29 -- (SquirrelMail authenticated user ebelluso-at-unito.it) 18, 29 -- by mail.unito.it with HTTP; 18, 29 -- Fri, 23 Jan 2009 10:58:51 +0100 (CET) 18, 29 -- Message-ID: {1973.130.192.111.68.1232704731.squirrel-at-mail.unito.it} 18, 29 -- Date: Fri, 23 Jan 2009 10:58:51 +0100 (CET) 18, 29 -- Subject: ICC asbestos session 18, 29 -- From: elena.belluso-at-unito.it 18, 29 -- To: Microscopy-at-microscopy.com 18, 29 -- User-Agent: SquirrelMail/1.4.2 18, 29 -- MIME-Version: 1.0 18, 29 -- Content-Type: text/plain;charset=iso-8859-1 18, 29 -- Content-Transfer-Encoding: 8bit 18, 29 -- X-Priority: 3 18, 29 -- Importance: Normal 18, 29 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv 18, 29 -- X-Inrete-Amavisjob-Service-Runned: 6 (n0N9wpOK026202) 18, 29 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (n0N9wpOK026202) ==============================End of - Headers==============================
Hi Allan, I've had a number of users in the past looking at nanotubes here in my lab, and one of them was interested in the internal microstructure. We ended up embedding the nanotubes in resin and thin-sectioning them. It worked pretty well, but the structure they were looking at was a little grosser than your's. If you think it's worth a shot, let me know and I'll dig out my files and find out exactly how we did it. Cheers, Jules
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
Dear Allan (and All), Apologies, but I should have clarified that the nanotubes that I embedded previously for thin-sectioning were carbon, not TiO2 (it's Friday and am a bit tired - should have read the subject header more carefully!). I have no experience of dealing with the latter, so could not say whether the embedding route would work or not. Cheers, Jules
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
Dear All, I've recently been using TAAB Low Viscosity (TLV) resin (since the sad demise of Spurr resin!) for embedding standard double-fixed cells and tissues and sometimes have problems achieving good contrast with routine uranyl acetate (UA) and Pb citrate post-staining. Has anyone else experienced the same problem and found a way to get over it? I guess alcoholic UA might be the way to go, but not sure how you rinse the grids after this. Thanks in advance for any advice, Julian
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
Dear All, I've recently been using TAAB Low Viscosity (TLV) resin (since the sad demise of Spurr resin!) for embedding standard double-fixed cells and tissues and sometimes have problems achieving good contrast with routine uranyl acetate (UA) and Pb citrate post-staining. Has anyone else experienced the same problem and found a way to get over it? I guess alcoholic UA might be the way to go, but not sure how you rinse the grids after this. Thanks in advance for any advice, Julian
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
Thank you! Thank you very very much! For explaining where the terminology "Depleted" comes from - I know that a number of us have been wondering that for a long time.
As well as taking the time to explain the general process for the manufacture of our Uranyl acetate.
On 8 Jan 2009 at 19:20, abesenyo-at-ibilabs.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both abesenyo-at-ibilabs.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: abesenyo-at-ibilabs.com } Name: Alex Besenyo PhD } } Organization: ibilabs } } Title-Subject: [Filtered] uranyl compounds are alpha emitters only } } Question: Question: } } Is it true that the stuff we use has been somehow } depleted, so that it isn't as radioactive as "real" uranyl } salts? Or is this yet another old wive's tale of EM?! } } Reply: } } When we manufacture these compounds we purchase the raw uranium in a } depleted state from the government. There is no chance for error } here. We do not use natural uranium. } } This means that the enrichable uranium U-235 has been removed. } The then U-238 which only emitts alpha radiation is procesed. } } The term "depleted" means that U-235 has been removed. } } If even by the slightest chance that U235 were present then every } alarm would go off in our facility because Beta and Gamma radiation } is detected. } } I hope this answers everybodies concerns. } } Our products are sold exclusively through a distributor network and } all of them have been instructed on this information. } } I only responded when I saw the original post and I had to respond } before it got out of control. } } Sincerely } Alex Besenyo PhD } } } Login Host: 74.173.69.139 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 17, 11 -- From zaluzec-at-microscopy.com Thu Jan 8 18:19:52 2009 } 17, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) } 17, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n090Jp6S032218 } 17, 11 -- for {microscopy-at-microscopy.com} ; Thu, 8 Jan 2009 18:19:52 -0600 } 17, 11 -- Mime-Version: 1.0 } 17, 11 -- Message-Id: {p06240809c58c4893ab36-at-[206.69.208.22]} } 17, 11 -- Date: Thu, 8 Jan 2009 18:19:48 -0600 } 17, 11 -- To: microscopy-at-microscopy.com } 17, 11 -- From: abesenyo-at-ibilabs.com (by way of MicroscopyListserver) } 17, 11 -- Subject: viaWWW: uranyl compounds are alpha emitters only } 17, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 23 10:06:56 2009 10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.69]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NG6tDK014674 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 23 Jan 2009 10:06:55 -0600 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0NG6v7U000367; 10, 25 -- Fri, 23 Jan 2009 11:06:57 -0500 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n0NG6j0H031024; 10, 25 -- Fri, 23 Jan 2009 11:06:45 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: "abesenyo-at-ibilabs.com" {abesenyo-at-ibilabs.com} 10, 25 -- Date: Fri, 23 Jan 2009 11:06:44 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] viaWWW: uranyl compounds are alpha emitters only 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {4979A4C4.10991.5D62DA3C-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200901090020.n090KkVO001188-at-ns.microscopy.com} 10, 25 -- References: {200901090020.n090KkVO001188-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69 ==============================End of - Headers==============================
I haven't used TAAB but have experience with Spurr and the recent low viscosity epoxy sold in the US by Electron Microscopy Sciences. Both are harder to stain than either "normal", Epon/Araldite like epoxies or acrylics. The reason is the higher degree of cross-linking.
(Not something you are asking, but do you really need to use a low viscosity resin? Besides the staining issues, they are also more of a health hazard.)
Yes, alcoholic UA will stain better, and you are correct, some caution is needed when rinsing afterwards. My favorite UA procedure for such cases is to use the stock of saturated UA in water and dilute it before each staining 1:1 with methanol. This way you don't need to worry about precipitate and still have high enough concentration of UA in 50% methanol, fresh each time. Stain for 5-10 minutes. I rinse first on a drop of 50% methanol, then 25% methanol, then a few droplets of water gently flowing from a disposable 3 ml pipette. It is a good general practice to avoid overwashing, both with UA and Pb staining. Too much washing won't get rid of the precipitate formed on sections but will destain.
Whenever I have such contrast issues, I also use Venable-Coggeshall [spelling?] recipe for the lead stain. I keep pre-weighed amounts in 15 ml Falcon tubes and make it fresh every such time (1-2 hours ahead of staining). Making it fresh is important - a stronger stain than "average" Reynolds, it does not store well. It really helps.
Finally, it helps to include en bloc UA treatment before embedding - either 2% UA in water before dehydration or 1.5% at the 70% ethanol step.
This topic has been covered quite a few times on this list. A good place to search the archives is http://www.biotech.ufl.edu/EM/tips/ You'll find more discussion there.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and not his employer. These opinions and experiences do not represent a consensus of the NIH scientific community. On the good side, this message is not confidential and can be freely shared and reproduced.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } I've recently been using TAAB Low Viscosity (TLV) resin } (since the } sad demise of Spurr resin!) for embedding standard double-fixed } cells and } tissues and sometimes have problems achieving good contrast with } routine } uranyl acetate (UA) and Pb citrate post-staining. Has anyone else } experienced the same problem and found a way to get over it? I guess } alcoholic UA might be the way to go, but not sure how you rinse the } grids } after this. } Thanks in advance for any advice, } Julian } } Dr. Julian R. Thorpe } (Office 2C9/Lab 2C11-13) } Electron Microscope Division, } The Sussex Centre for Advanced Microscopy, } John Maynard-Smith Building, } School of Life Sciences, } University of Sussex, } Falmer, } Brighton BN1 9QG } Tel.: ext +44 (0)1273 877585 } int 7585 } } URLs: } (home) } http://www.sussex.ac.uk/biology/profile2686.html } (lab) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm } (research) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm } } ==============================Original } Headers============================== } 3, 24 -- From bafg3-at-sussex.ac.uk Fri Jan 23 09:57:50 2009 } 3, 24 -- Received: from sivits.uscs.susx.ac.uk } (sivits.uscs.susx.ac.uk [139.184.14.88]) } 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n0NFvnrr023009 } 3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 } 09:57:49 -0600 } 3, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]: } 2132) } 3, 24 -- by sivits.uscs.susx.ac.uk with esmtp (Exim 4.64) } 3, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} ) } 3, 24 -- id KDXLQ4-000E70-GX } 3, 24 -- for microscopy-at-microscopy.com; Fri, 23 Jan 2009 } 15:58:52 +0000 } 3, 24 -- Date: Fri, 23 Jan 2009 15:57:47 -0000 } 3, 24 -- To: microscopy-at-microscopy.com } 3, 24 -- Subject: Biological TEM: TAAB Low Viscosity (TLV) resin- } embedded specimen } 3, 24 -- section post-staining } 3, 24 -- Message-ID: {70653603.1232726267-at-ls0130.lifesci.susx.ac.uk} } 3, 24 -- Originator-Info: login-token=Mulberry:01R } +B5y6lB3zJ7INklsER1U+4+u0Xk8qg7dVXk; } 3, 24 -- token_authority=postmaster-at-central.susx.ac.uk } 3, 24 -- X-Mailer: Mulberry/2.0.8 (Win32) } 3, 24 -- MIME-Version: 1.0 } 3, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed } 3, 24 -- Content-Transfer-Encoding: 7bit } 3, 24 -- Content-Disposition: inline } 3, 24 -- X-Sussex: true } 3, 24 -- X-Sussex-transport: remote_smtp_rew } 3, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk} } ==============================End of - } Headers==============================
==============================Original Headers============================== 13, 23 -- From vladislav_speransky-at-nih.gov Fri Jan 23 11:51:55 2009 13, 23 -- Received: from out3.smtp.messagingengine.com (out3.smtp.messagingengine.com [66.111.4.27]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NHptkR032232 13, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 11:51:55 -0600 13, 23 -- Received: from compute2.internal (compute2.internal [10.202.2.42]) 13, 23 -- by out1.fastmail.fm (Postfix) with ESMTP id 1639125590A 13, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:51:55 -0500 (EST) 13, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161]) 13, 23 -- by compute2.internal (MEProxy); Fri, 23 Jan 2009 12:51:55 -0500 13, 23 -- X-Sasl-enc: FXVmF70t0dxZCDCFl1RNa5IvlWm6KzyeCgDcCBcn7gbo 1232733114 13, 23 -- Received: from [192.168.0.5] (unknown [96.241.28.40]) 13, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id B493720495 13, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:51:54 -0500 (EST) 13, 23 -- Message-Id: {0291FDBD-A5DB-44E1-9AF8-7DA14EB03EFC-at-nih.gov} 13, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 13, 23 -- To: Microscopy-at-microscopy.com 13, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 23 -- Content-Transfer-Encoding: 7bit 13, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 13, 23 -- Subject: Fwd: [Microscopy] Biological TEM: TAAB Low Viscosity (TLV) resin-embedded specimen 13, 23 -- Date: Fri, 23 Jan 2009 12:51:53 -0500 13, 23 -- References: {200901231558.n0NFwfqe025306-at-ns.microscopy.com} 13, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I am hoping someone on the list can help me I need to record video from a microscope plus a simultaneous audio commentary, and output both to a AVI/MPG/MOV file. The camera must fit on a microscope C-mount. I have posted on this before and got recommendations of cameras that produce AVI files via USB connection, but incorporating live audio so far, is an unresolved problem. It would seem there is a lot of software that will add a sound track later but not live.
I can think of two approaches 1 software: Take a fairly inexpensive camera (Lumenera for instance) interface to a computer, + a standard microphone, and have some software create the AVI file from both inputs. - So far I haven't found software that will do this at least in real time.
2 Hardware: Take a camera that has built in sound, basically a camcorder, and mount it on a microscope via a C-mount. - I don't know of any camcorders that have c-mount adapters, also I'm not sure if they can be connected to a computer via USB and produce AVI files.
I would rather not go the route of outputting a TV signal to the computer and the adding a digital frame grabbing card to convert the video signal to digital.
Suggestions would be really appreciated
Thanks Lloyd
Dr. Lloyd Williams Dept of Biology Hunter College 695 Park Ave New York, NY 10021 ph (212) 650 3872 fx (212) 650 3656
==============================Original Headers============================== 14, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 23 12:04:35 2009 14, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34]) 14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NI4Y5A013756 14, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:04:34 -0600 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 14, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id 14, 26 -- 8.1.336.0; Fri, 23 Jan 2009 13:04:28 -0500 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 14, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 23 Jan 2009 14, 26 -- 13:04:55 -0500 14, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} 14, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 26 -- Date: Fri, 23 Jan 2009 13:04:54 -0500 14, 26 -- Subject: Realtime Audio and Vieo For a C-mount Microscope 14, 26 -- Thread-Topic: Realtime Audio and Vieo For a C-mount Microscope 14, 26 -- Thread-Index: Acl9gio1II/B79NXTe+PQqYZ6YQzbA== 14, 26 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE3E305B1893-at-Xchange5.bio.hunter.cuny.edu} 14, 26 -- Accept-Language: en-US 14, 26 -- Content-Language: en-US 14, 26 -- X-MS-Has-Attach: 14, 26 -- X-MS-TNEF-Correlator: 14, 26 -- acceptlanguage: en-US 14, 26 -- Content-Type: text/plain; charset="iso-8859-1" 14, 26 -- MIME-Version: 1.0 14, 26 -- Content-Transfer-Encoding: 8bit 14, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NI4Y5A013756 ==============================End of - Headers==============================
I have been thinking.....I have a jvc digital movie camera/recorder with a firewire output that I can connect directly to my computer, and record the results with Windows Media Maker (I think). This includes both the video and the audio.
As another, inexpensive alternative, what about the "Flip Camera"? It has a usb connector, and might work. The trick is to interface it with the microscope, but I would imagine that you could mount it on the eyepiece and adjust focus to match.
Joel
On 23 Jan 2009 at 12:11, Williams-at-GENECTR.HUNTER.CUNY.EDU wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am hoping someone on the list can help me } I need to record video from a microscope plus a simultaneous audio commentary, and output both to a AVI/MPG/MOV file. The camera must fit on a microscope C-mount. } I have posted on this before and got recommendations of cameras that produce AVI files via USB connection, but incorporating live audio so far, is an unresolved problem. } It would seem there is a lot of software that will add a sound track later but not live. } } I can think of two approaches } 1 software: Take a fairly inexpensive camera (Lumenera for instance) interface to a computer, + a standard microphone, and have some software create the AVI file from both inputs. - So far I haven't found software that will do this at least in real time. } } 2 Hardware: Take a camera that has built in sound, basically a camcorder, and mount it on a microscope via a C-mount. - I don't know of any camcorders that have c-mount adapters, also I'm not sure if they can be connected to a computer via USB and produce AVI files. } } I would rather not go the route of outputting a TV signal to the computer and the adding a digital frame grabbing card to convert the video signal to digital. } } Suggestions would be really appreciated } } Thanks } Lloyd } } } } } } } Dr. Lloyd Williams } Dept of Biology } Hunter College } 695 Park Ave } New York, NY 10021 } ph (212) 650 3872 } fx (212) 650 3656 } } } } ==============================Original Headers============================== } 14, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 23 12:04:35 2009 } 14, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34]) } 14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NI4Y5A013756 } 14, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:04:34 -0600 } 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by } 14, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id } 14, 26 -- 8.1.336.0; Fri, 23 Jan 2009 13:04:28 -0500 } 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by } 14, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 23 Jan 2009 } 14, 26 -- 13:04:55 -0500 } 14, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} } 14, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 14, 26 -- Date: Fri, 23 Jan 2009 13:04:54 -0500 } 14, 26 -- Subject: Realtime Audio and Vieo For a C-mount Microscope } 14, 26 -- Thread-Topic: Realtime Audio and Vieo For a C-mount Microscope } 14, 26 -- Thread-Index: Acl9gio1II/B79NXTe+PQqYZ6YQzbA== } 14, 26 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE3E305B1893-at-Xchange5.bio.hunter.cuny.edu} } 14, 26 -- Accept-Language: en-US } 14, 26 -- Content-Language: en-US } 14, 26 -- X-MS-Has-Attach: } 14, 26 -- X-MS-TNEF-Correlator: } 14, 26 -- acceptlanguage: en-US } 14, 26 -- Content-Type: text/plain; charset="iso-8859-1" } 14, 26 -- MIME-Version: 1.0 } 14, 26 -- Content-Transfer-Encoding: 8bit } 14, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NI4Y5A013756 } ==============================End of - Headers==============================
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 13, 43 -- From joelsheffield-at-gmail.com Fri Jan 23 13:35:52 2009 13, 43 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.144]) 13, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NJZpjk031195 13, 43 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 13:35:51 -0600 13, 43 -- Received: by qw-out-1920.google.com with SMTP id 4so1045808qwk.54 13, 43 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 11:35:51 -0800 (PST) 13, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 43 -- d=gmail.com; s=gamma; 13, 43 -- h=domainkey-signature:received:received:from:to:date:mime-version 13, 43 -- :subject:message-id:priority:in-reply-to:references:x-mailer 13, 43 -- :content-type:content-transfer-encoding:content-description; 13, 43 -- bh=2n1YesDXumMSS28mxhX9nRmsuMeXJg5Z/y/aM39mq1k=; 13, 43 -- b=hABYOBBq6VdId/oWPsrlu7zYdId9BtgmsHUdAapFB/qWY/wQ+iNLP6XYchbX6WaElP 13, 43 -- DsQB1nA1xr5URxhqcTFZq2w/4nkrZ80komcuOwBPh/HkQlMCNMGkXZnUYqUpuqU1+itE 13, 43 -- l0/gU/o5L2ToHyjD56iejIMZjcvUXbJbkqXo8= 13, 43 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 43 -- d=gmail.com; s=gamma; 13, 43 -- h=from:to:date:mime-version:subject:message-id:priority:in-reply-to 13, 43 -- :references:x-mailer:content-type:content-transfer-encoding 13, 43 -- :content-description; 13, 43 -- b=b3O4txafhkHQFmnlsRh+OyCsdtcJLEHZHNWmRm2T8iwRRtA9eWIQe75jQL8cgPy0eV 13, 43 -- ZV4hEnccIKk+IqEiI0FWbCnS4zcYB/qLmQH3MH88Z/OpBI+UGjSnPQeeUoDloDhyHml9 13, 43 -- RJbKzfjie/LBOJPJXInuN+MR+FfbV6u9vSdDc= 13, 43 -- Received: by 10.229.73.209 with SMTP id r17mr2177478qcj.30.1232739351146; 13, 43 -- Fri, 23 Jan 2009 11:35:51 -0800 (PST) 13, 43 -- Received: from ?155.247.98.40? (jbs.bio.temple.edu [155.247.98.40]) 13, 43 -- by mx.google.com with ESMTPS id 30sm11136473yxk.47.2009.01.23.11.35.49 13, 43 -- (version=SSLv3 cipher=RC4-MD5); 13, 43 -- Fri, 23 Jan 2009 11:35:50 -0800 (PST) 13, 43 -- From: joelsheffield-at-gmail.com 13, 43 -- To: Williams-at-GENECTR.HUNTER.CUNY.EDU, microscopy-at-microscopy.com 13, 43 -- Date: Fri, 23 Jan 2009 14:35:52 -0500 13, 43 -- MIME-Version: 1.0 13, 43 -- Subject: Re: [Microscopy] Realtime Audio and Vieo For a C-mount Microscope 13, 43 -- Message-ID: {4979D5C8.9197.2BA61D9B-at-joelsheffield.gmail.com} 13, 43 -- Priority: normal 13, 43 -- In-reply-to: {200901231811.n0NIB664027188-at-ns.microscopy.com} 13, 43 -- References: {200901231811.n0NIB664027188-at-ns.microscopy.com} 13, 43 -- X-mailer: Pegasus Mail for Windows (4.41) 13, 43 -- Content-type: text/plain; charset=ISO-8859-1 13, 43 -- Content-description: Mail message body 13, 43 -- Content-Transfer-Encoding: 8bit 13, 43 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id n0NJZpjk031195 ==============================End of - Headers==============================
Hi Hong, We have four large histoknives, usually one for trimming, two for everyday use, and one in perfect shape for when one of the others has to go away for resharpening. They are used to cut sections up to 2 microns thick, of quite large blockfaces - up to 3 mm across. They get resharpened at least once a year. Like Stephane, I'll never go back to routine glass knife use, the diamond knives save so much time. We only go back to glass for training and if the tissue might damage the diamond (chunks of rock in soil around roots, for example...).
cheers, Roseamry
On 22/01/09 7:47 PM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } ---------------------------------------------------------------------- } ------ } } Hi Hong! } } We have a histo knife, however we don't cut thicker than 500nm and not } on a routine basis. } I have been said that like an ultraknife, its lifetime mainly depends } on what you cut. Cut butter and it will survive you. } Cut nanoparticles and quantum dots and it will probably not survive } your grant. Cutting soft tissue in resin does probably not significantly affect it. } Personally I couldn't imagine regularly semi-thin sectionning without } histoknife, it is so comfortable. Maybe I am a luxus freak :-) } } Regards, } Stephane } } } } ----- Original Message ---- } X-from: "hyi-at-emory.edu" {hyi-at-emory.edu} } To: nizets2-at-yahoo.com } Sent: Thursday, January 22, 2009 6:34:37 AM } Subject: [Microscopy] Histo diamond knife } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } ---------------------------------------------------------------------- } ------ } } Dear All: } } We have been contemplating about purchasing a 8.0 mm Histo diamond } knif= e for semi-thin (0.5-0.7=B5m) sectioning. Can someone out there } with exper= ience comment on how long (or how many blocks) I should } expect a Histo diam= ond knife to last? I have no problem producing } high quality semi-thin sect= ions with glass knives, but am hoping a } diamond knife would save us some ti= me. Thank you in advance. } } Hong } Emory EM } } } This e-mail message (including any attachments) is for the sole use of } the intended recipient(s) and may contain confidential and privileged } information. If the reader of this message is not the intended } recipient, you are hereby notified that any dissemination, } distribution or copying of this message (including any attachments) is } strictly prohibited. } } If you have received this message in error, please contact the sender } by reply e-mail message and destroy all copies of the original message } (including attachments). } } } ==============================Original } Headers============================== } 7, 32 -- From hyi-at-emory.edu Wed Jan 21 23:30:05 2009 7, 32 -- } Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) 7, } 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n0M5U5Rv023498 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jan 2009 } 23:30:05 -0600 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net } (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 32 -- by } mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n0M5U0qO021756 7, 32 -- } for {Microscopy-at-microscopy.com} ; Thu, 22 Jan 2009 00:30:00 -0500 7, 32 } -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by 7, } 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft
I replied to a similar question last week where I used a Q-see camera that I hooked up to my video camera. I can then either record it on the video camera or route it to the computer or a digital recorder. What I didn't say is that I also hooked up audio with it. The camera that I am using is a Canon Elura 85. I use the same cord that came with the camera to hook to the camera and audio with RCA plugs on one end and the three connector mini-plug that goes into the camera. I set the camera on VCR mode and there's no problem. I can talk in my gravelly voice and can be heard while viewing the recorded show. That is a problem.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: Williams-at-GENECTR.HUNTER.CUNY.EDU [mailto:Williams-at-GENECTR.HUNTER.CUNY.EDU] Sent: Friday, January 23, 2009 10:07 AM To: Walck-at-SouthBayTech.com
I am hoping someone on the list can help me I need to record video from a microscope plus a simultaneous audio commentary, and output both to a AVI/MPG/MOV file. The camera must fit on a microscope C-mount. I have posted on this before and got recommendations of cameras that produce AVI files via USB connection, but incorporating live audio so far, is an unresolved problem. It would seem there is a lot of software that will add a sound track later but not live.
I can think of two approaches 1 software: Take a fairly inexpensive camera (Lumenera for instance) interface to a computer, + a standard microphone, and have some software create the AVI file from both inputs. - So far I haven't found software that will do this at least in real time.
2 Hardware: Take a camera that has built in sound, basically a camcorder, and mount it on a microscope via a C-mount. - I don't know of any camcorders that have c-mount adapters, also I'm not sure if they can be connected to a computer via USB and produce AVI files.
I would rather not go the route of outputting a TV signal to the computer and the adding a digital frame grabbing card to convert the video signal to digital.
Suggestions would be really appreciated
Thanks Lloyd
Dr. Lloyd Williams Dept of Biology Hunter College 695 Park Ave New York, NY 10021 ph (212) 650 3872 fx (212) 650 3656
==============================Original Headers============================== 14, 26 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Fri Jan 23 12:04:35 2009 14, 26 -- Received: from genectr.hunter.cuny.edu (xchange6.hunter.cuny.edu [146.95.150.34]) 14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NI4Y5A013756 14, 26 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 12:04:34 -0600 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu (146.95.150.45) by 14, 26 -- genectr.hunter.cuny.edu (146.95.150.34) with Microsoft SMTP Server (TLS) id 14, 26 -- 8.1.336.0; Fri, 23 Jan 2009 13:04:28 -0500 14, 26 -- Received: from Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) by 14, 26 -- Xchange5.bio.hunter.cuny.edu ([146.95.150.45]) with mapi; Fri, 23 Jan 2009 14, 26 -- 13:04:55 -0500 14, 26 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} 14, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 26 -- Date: Fri, 23 Jan 2009 13:04:54 -0500 14, 26 -- Subject: Realtime Audio and Vieo For a C-mount Microscope 14, 26 -- Thread-Topic: Realtime Audio and Vieo For a C-mount Microscope 14, 26 -- Thread-Index: Acl9gio1II/B79NXTe+PQqYZ6YQzbA== 14, 26 -- Message-ID: {A5D6F41FED41B74894FDDFBA77633BCE3E305B1893-at-Xchange5.bio.hunter.cuny.edu} 14, 26 -- Accept-Language: en-US 14, 26 -- Content-Language: en-US 14, 26 -- X-MS-Has-Attach: 14, 26 -- X-MS-TNEF-Correlator: 14, 26 -- acceptlanguage: en-US 14, 26 -- Content-Type: text/plain; charset="iso-8859-1" 14, 26 -- MIME-Version: 1.0 14, 26 -- Content-Transfer-Encoding: 8bit 14, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NI4Y5A013756 ==============================End of - Headers==============================
Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008 5:29 PM
==============================Original Headers============================== 25, 23 -- From walck-at-southbaytech.com Fri Jan 23 14:16:39 2009 25, 23 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82]) 25, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKGb3C027111 25, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:16:38 -0600 25, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 25, 23 -- (authenticated bits=0) 25, 23 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n0NKGXue004584; 25, 23 -- Fri, 23 Jan 2009 14:16:34 -0600 25, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 25, 23 -- To: {Microscopy-at-microscopy.com} 25, 23 -- Cc: {Williams-at-GENECTR.HUNTER.CUNY.EDU} 25, 23 -- Subject: RE: [Microscopy] Realtime Audio and Vieo For a C-mount Microscope 25, 23 -- Date: Fri, 23 Jan 2009 12:16:50 -0800 25, 23 -- Message-ID: {ADF988ACE1504B72A4AD2742ABEC7803-at-dynamicbl8uno3} 25, 23 -- MIME-Version: 1.0 25, 23 -- Content-Type: text/plain; 25, 23 -- charset="iso-8859-1" 25, 23 -- X-Mailer: Microsoft Office Outlook 11 25, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 25, 23 -- Thread-Index: Acl9hXQtpzSUhkiNSKydCvZDh9oUZAAEP+QA 25, 23 -- In-Reply-To: {200901231807.n0NI7OFa018790-at-ns.microscopy.com} 25, 23 -- Content-Transfer-Encoding: 8bit 25, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NKGb3C027111 ==============================End of - Headers==============================
I have seen some mentions here of methods to enhance fixation and embedding of nematodes. I just watched my vinegar eels (T. acetii) swim happily for } 1hr in the [2% glutaraldehyde + 4% paraformaldhyde + 0.2M cacodylate, pH 7.4] fixative that was reportedly used in some work with beautiful results - that work reported a 1hr fixation; surely something is wrong here. I have seen mention of slicing with a razor blade (can it really be done? How? These vinegar eels are tiny and very wiggly); penetrating with a pulled micropipette; cryo fixation; enzymatic digestion of the cuticle (is this done after fixation?); and finally, laser beams - what type of laser is required - can this be done with a confocal or does it need a higher power or other wavelengths? I have some early/mid-1970's papers that have beautiful ultrastructure with no special methods mentioned at all - maybe not complete disclosure of the details?
I am wondering if electroporation might be any use to allow quicker permeabilization of a bulk sample. Does anyone know if electroporation has ever been used - is this a terrible idea?
I would greatly appreciate any protocols, or references to books or papers dealing with the nitty-gritty details of such tiny impermeable preparations.
Thanks!
Dale Callaham
==============================Original Headers============================== 6, 20 -- From dac-at-research.umass.edu Fri Jan 23 14:21:30 2009 6, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKLUjK004106 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:21:30 -0600 6, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 20 -- (authenticated bits=0) 6, 20 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0NKLT93017629 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 15:21:30 -0500 6, 20 -- Message-ID: {497A2712.6060305-at-research.umass.edu} 6, 20 -- Date: Fri, 23 Jan 2009 15:22:42 -0500 6, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 20 -- Reply-To: dac-at-research.umass.edu 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 6, 20 -- MIME-Version: 1.0 6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 20 -- Subject: Nematode permeabilization for fixation and embedding 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
I've been working with a polished copper bar and found some inclusions. EDS shows nothing but copper and of course it can't detect beryllium. It occurred to me that beryllium has such a low backscatter coefficient as compared to copper and areas enriched in berllium should appear dark in compo mode.
Would it be reasonable to say that any dark areas in BS imaging what show only copper could have beryllium enrichment? And while I'm on the subject, does any one have a copy of the condensed micro-chemical test for beryllium that Dr. McCone published years ago in the Microscope? If so could I get a copy?
Thanks, Frank Karl Lincoln Electric
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==============================Original Headers============================== 6, 21 -- From frank_karl-at-lincolnelectric.com Fri Jan 23 14:51:11 2009 6, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKpA31022903 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:51:10 -0600 6, 21 -- Subject: Searching for beryllium in all the wrong places..... 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 6, 21 -- Message-ID: {OF758F0713.7A6F5B73-ON85257547.007151A5-85257547.007285EB-at-lincolnelectric.com} 6, 21 -- Date: Fri, 23 Jan 2009 15:51:00 -0500 6, 21 -- From: Frank_Karl-at-lincolnelectric.com 6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- CD-MIME complete at 01/23/2009 03:50:55 PM, 6, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- Serialize complete at 01/23/2009 03:50:55 PM 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
I would suggest using alcohol (better adhesion) for deposition of nanotubes on carbon film (only carbon, to decrease charging). Usually you can find a lot of nanotubes poking out of edges of clamps, so good dispersion of specimens in not a mandatory thing. You can also try to deposit (with alcohol) clamps of nanotubes directly on Cu grid without any film. Sometimes it can give better results (if you can find these clamps tracing outline of grid) - contrast and resolution are better without film.
Good luck,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} } Title-Subject: [Filtered] TEM of TiO2 nanotubes } } Question: Hi all } } I have been working with a researcher here looking at some } TiO2 Nanotubes in the TEM. The researcher wants to see if } his nanotubes are hollow or not. We are looking for a core } of around 5 nm. } } Being a biology lab we do not have any experience with such samples. } I have tried dusting the fragments (provided in powder form } from scrappings off a Ti plate) onto carbon/formvar grids } and I have tried drying them onto a grid from a suspension in } ethanol. The solvent method proved more successful at } getting particles onto the film than the dusting method } however in both cases they tend to be clumped. } } The problem I have is the clumps must be heating up in the } beam. I as soon as I start to increase the magnification to } explore the edges the sample disappears and I am left with a } hole in the film. } } I am pushing a 100kV instrument so I suspect this may have } something to with it also. } } A search of the literature indicates that a lot of people are } investigating TiO2 nanotubes in the TEM but nothing I have } found so far talks about how the nanotubes are got onto a } grid in a usable form to image. lots of info about making nanotubes. } } Any thoughts or suggestions would be appreciated. } } Regards } } Allan } } } Allan Mitchell } Otago Centre for Electron Microscopy } Department of Anatomy and Structural Biology School of } Medical Sciences P.O. Box 913 Dunedin New Zealand } } Phone (03) 479 5642 or 479 7301 } Fax (03) 479 5086 or 479 7254 } } EM Centre: http://ocem.otago.ac.nz/ } Confocal Centre: http://occm.otago.ac.nz/ } Department: http://anatomy.otago.ac.nz/ } } Login Host: 139.80.40.92 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 18, 11 -- From zaluzec-at-microscopy.com Thu Jan 22 08:11:29 } 2009 18, 11 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } 18, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n0MEBTKc026853 } 18, 11 -- for {microscopy-at-microscopy.com} ; Thu, 22 Jan } 2009 08:11:29 -0600 } 18, 11 -- Mime-Version: 1.0 } 18, 11 -- Message-Id: {p06240801c59e2edb6749-at-[206.69.208.22]} } 18, 11 -- Date: Thu, 22 Jan 2009 08:11:28 -0600 18, 11 -- To: } microscopy-at-microscopy.com 18, 11 -- From: } allan.mitchell-at-stonebow.otago.ac.nz (by way of } MicroscopyListserver) 18, 11 -- Subject: viaWWW: TEM of TiO2 } nanotubes 18, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 25 -- From DusevichV-at-umkc.edu Fri Jan 23 14:56:05 2009 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (smtp1.exchange.umkc.edu [134.193.143.155]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKu4SQ031946 8, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:56:05 -0600 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 25 -- Fri, 23 Jan 2009 14:56:04 -0600 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="us-ascii" 8, 25 -- Subject: RE: [Microscopy] viaWWW: TEM of TiO2 nanotubes 8, 25 -- Date: Fri, 23 Jan 2009 14:56:03 -0600 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7BD-at-KC-MSX1.kc.umkc.edu} 8, 25 -- In-Reply-To: {200901221411.n0MEBvx8027718-at-ns.microscopy.com} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: [Microscopy] viaWWW: TEM of TiO2 nanotubes 8, 25 -- thread-index: Acl8m2YmfonpwTiFSwivxJg30W7fNQA/8LLg 8, 25 -- References: {200901221411.n0MEBvx8027718-at-ns.microscopy.com} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: {allan.mitchell-at-stonebow.otago.ac.nz} , {Microscopy-at-microscopy.com} 8, 25 -- X-OriginalArrivalTime: 23 Jan 2009 20:56:04.0271 (UTC) FILETIME=[0158E3F0:01C97D9D] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NKu4SQ031946 ==============================End of - Headers==============================
Dear Dale- I've never worked with your beasties, but have been doing both EM and confocal on sea squirt (Ciona intestinalis) larvae with one of the PIs here. She uses electroporation to get her GFP constructs into the very young larvae then lets them mature to the stage she is interested in studying...so that method along does not kill them (usually), but does "open them up" to things. For the EM studies, we used 2.5% glut, 4% pfa in buffer at 4 degrees overnight, then proceeded pretty much as normal, except that we did 2 changes at each alcohol level and prolonged the infiltration (over 2 days). We used Spurr's at the time. The C. elegans people love high pressure freezing followed by freeze substitution. When it works, it is gorgeous. Lee
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-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Yes, beryllium rich phases should exhibit lower atomic number contrast via BSe. If you have a beryllium-copper alloy that has been precipitation hardened, the CuBe phase should be dispersed evenly throughout the matrix as numerous spherical precipitates. They should also appear gray in optical brightfield and full wave polarized light if I recall.
If you are seeing isolated inclusions, they may be oxides, which will appear ruby red in full wave polarized light.
Care to provide a link to an image?
Joseph M. Oparowski Research Engineer Transducer Research The Mountain M/S 470 Framingham, MA 01701-9168
-----Original Message----- X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com] Sent: Friday, January 23, 2009 3:59 PM To: Oparowski, Joseph
I've been working with a polished copper bar and found some inclusions. EDS shows nothing but copper and of course it can't detect beryllium. It occurred to me that beryllium has such a low backscatter coefficient as compared to copper and areas enriched in berllium should appear dark in compo mode.
Would it be reasonable to say that any dark areas in BS imaging what show only copper could have beryllium enrichment? And while I'm on the subject, does any one have a copy of the condensed micro-chemical test for beryllium that Dr. McCone published years ago in the Microscope? If so could I get a copy?
Thanks, Frank Karl Lincoln Electric
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==============================Original Headers============================== 6, 21 -- From frank_karl-at-lincolnelectric.com Fri Jan 23 14:51:11 2009 6, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKpA31022903 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:51:10 -0600 6, 21 -- Subject: Searching for beryllium in all the wrong places..... 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 6, 21 -- Message-ID: {OF758F0713.7A6F5B73-ON85257547.007151A5-85257547.007285EB-at-lincolnelectric.com} 6, 21 -- Date: Fri, 23 Jan 2009 15:51:00 -0500 6, 21 -- From: Frank_Karl-at-lincolnelectric.com 6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- CD-MIME complete at 01/23/2009 03:50:55 PM, 6, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- Serialize complete at 01/23/2009 03:50:55 PM 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From Joseph_Oparowski-at-bose.com Fri Jan 23 15:13:12 2009 20, 26 -- Received: from usmamx02.bose.com (usmamx02.bose.com [139.68.146.59]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NLDBYE031580 20, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 23 Jan 2009 15:13:11 -0600 20, 26 -- X-PMWin-Version: 3.0.1.0, Antivirus-Engine: 2.80.0, Antivirus-Data: 4.35E 20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 20, 26 -- Received: from usmaexht02.bose.com ([10.100.10.8]) by usmamx02.bose.com with Microsoft SMTPSVC(6.0.3790.3959); Fri, 23 Jan 2009 16:13:11 -0500 20, 26 -- Received: from usmaexmb01.bose.com ([10.101.20.21]) by usmaexht02.bose.com ([10.102.20.24]) with mapi; Fri, 23 Jan 2009 16:13:11 -0500 20, 26 -- From: "Oparowski, Joseph" {Joseph_Oparowski-at-bose.com} 20, 26 -- To: {Microscopy-at-Microscopy.Com} 20, 26 -- Date: Fri, 23 Jan 2009 16:13:09 -0500 20, 26 -- Subject: FW: [Microscopy] Searching for beryllium in all the wrong places..... 20, 26 -- Thread-Topic: [Microscopy] Searching for beryllium in all the wrong places..... 20, 26 -- thread-index: Acl9nX62f6VSMWucTuS9daFPGU57owAAIKxQ 20, 26 -- Message-ID: {85D771575E0D11479A44687ED1A2F6006372E8-at-usmaexmb01.bose.com} 20, 26 -- Accept-Language: en-US 20, 26 -- Content-Language: en-US 20, 26 -- X-MS-Has-Attach: 20, 26 -- X-MS-TNEF-Correlator: 20, 26 -- acceptlanguage: en-US 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- MIME-Version: 1.0 20, 26 -- X-OriginalArrivalTime: 23 Jan 2009 21:13:11.0545 (UTC) FILETIME=[65A67690:01C97D9F] 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NLDBYE031580 ==============================End of - Headers==============================
Most assuredly it is possible to detect Be with EDS - which obviously must have a UTW detector. Although we were using biological materials that are mostly carbon, it is absolutely no problem with higher Z materials such as Al. I haven't tried Cu but as long as there are no other overlapping peaks around 0.11 Kev it should not be a problem. One caveat: you do have to be careful to tweak the "threshold" for the pulse processor to minimize the potential detector "noise" that can creep in! Here is a reference of ours from a few years ago:
Butnor KJ, Sporn TA, Ingram P, Gunasegaram S, Pinto JF, Roggli VL. Beryllium detection in human lung tissue using electron probe X-ray microanalysis, Mod Pathol. 2003 Nov;16(11):1171-7
Feel free to contact me off-line if you would like to discuss further.
Peter
} } } I've been working with a polished copper bar and found some inclusions. } EDS shows nothing but copper and of course it can't detect beryllium. It } occurred to me that beryllium has such a low backscatter coefficient as } compared to copper and areas enriched in berllium should appear dark in } compo mode. } } Would it be reasonable to say that any dark areas in BS imaging what show } only copper could have beryllium enrichment? And while I'm on the subject, } does any one have a copy of the condensed micro-chemical test for beryllium } that Dr. McCone published years ago in the Microscope? If so could I get a } copy? } } Thanks, } Frank Karl } Lincoln Electric } }
-- Peter Ingram Sr. Physicist Adj. Professor of Pathology, Duke University Medical Center Box 90319 LaSalle Street Extension DURHAM NC USA 27708-0319
Frank; It would be a big surprise to me to see beryllium in a bar, as it is normally used to precipitation harden copper for springs. Also, the precipitates would be expected to be very small and well dispersed, not in inclusions.
John Mardinly, Numonyx
-----Original Message----- X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com] Sent: Friday, January 23, 2009 12:58 PM To: MARDINLY, A
I've been working with a polished copper bar and found some inclusions. EDS shows nothing but copper and of course it can't detect beryllium. It occurred to me that beryllium has such a low backscatter coefficient as compared to copper and areas enriched in berllium should appear dark in compo mode.
Would it be reasonable to say that any dark areas in BS imaging what show only copper could have beryllium enrichment? And while I'm on the subject, does any one have a copy of the condensed micro-chemical test for beryllium that Dr. McCone published years ago in the Microscope? If so could I get a copy?
Thanks, Frank Karl Lincoln Electric
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 6, 21 -- From frank_karl-at-lincolnelectric.com Fri Jan 23 14:51:11 2009 6, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKpA31022903 6, 21 -- for {microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:51:10 -0600 6, 21 -- Subject: Searching for beryllium in all the wrong places..... 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 6, 21 -- Message-ID: {OF758F0713.7A6F5B73-ON85257547.007151A5-85257547.007285EB-at-lincolnelectr ic.com} 6, 21 -- Date: Fri, 23 Jan 2009 15:51:00 -0500 6, 21 -- From: Frank_Karl-at-lincolnelectric.com 6, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- CD-MIME complete at 01/23/2009 03:50:55 PM, 6, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 21 -- 07, 2008) at 01/23/2009 03:50:55 PM, 6, 21 -- Serialize complete at 01/23/2009 03:50:55 PM 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 14, 29 -- From A.MARDINLY-at-numonyx.com Fri Jan 23 15:59:43 2009 14, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NLxgN0024318 14, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 23 Jan 2009 15:59:43 -0600 14, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23]) 14, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 0300C414460; 14, 29 -- Fri, 23 Jan 2009 16:26:52 -0500 (EST) 14, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 14, 29 -- Fri, 23 Jan 2009 16:59:41 -0500 14, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 29 -- Content-class: urn:content-classes:message 14, 29 -- MIME-Version: 1.0 14, 29 -- Content-Type: text/plain; 14, 29 -- charset="us-ascii" 14, 29 -- Subject: RE: [Microscopy] Searching for beryllium in all the wrong places..... 14, 29 -- Date: Fri, 23 Jan 2009 16:59:40 -0500 14, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9D60A71-at-EXDRESBENMX012.numonyx.local} 14, 29 -- In-Reply-To: {200901232057.n0NKvqmA005293-at-ns.microscopy.com} 14, 29 -- X-MS-Has-Attach: 14, 29 -- X-MS-TNEF-Correlator: 14, 29 -- Thread-Topic: [Microscopy] Searching for beryllium in all the wrong places..... 14, 29 -- Thread-Index: Acl9nVd8ZnX5e4STQCiwd7fzN8KeHAACBpBA 14, 29 -- References: {200901232057.n0NKvqmA005293-at-ns.microscopy.com} 14, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 14, 29 -- To: {Frank_Karl-at-lincolnelectric.com} 14, 29 -- Cc: {Microscopy-at-Microscopy.com} 14, 29 -- X-OriginalArrivalTime: 23 Jan 2009 21:59:41.0752 (UTC) FILETIME=[E4BE4380:01C97DA5] 14, 29 -- Content-Transfer-Encoding: 8bit 14, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0NLxgN0024318 ==============================End of - Headers==============================
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Question: Dear All, I've recently been using TAAB Low Viscosity (TLV) resin (since Spurr resin became unavailable, sadly!). Anyway, I've had some problems with some specimens in achieving good contrast with routine uranyl acetate (UA) and Pb citrate post-staining. Has anyone else found this to be the case? If so, and you've managed to get around this problem, I'd be happy to hear from you. I guess alcoholic UA might be the way to go, but I've often had problems rinsing this off properly. Thanks for any advice in advance, Julian
Dr. Julian R. Thorpe Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
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Title-Subject: [Filtered] First announcement: Workshop on Structural & Computational Biomedical Informatics and Cryo-EM
Question: Professor Wah Chiu Baylor College of Medicine, JEOL (UK) Ltd and The National Institute for Biological Standards and Control are holding the Workshop on Structural & Computational Biomedical Informatics and Cryo-EM. The workshop is a 5 day residential course on June 8th ñJune 12th 2009. Topics to be covered include: Specimen preparation, electron optics involved, configuration of the TEM and understanding specimen beam interaction, Data size ñ Assessment and Data Processing, Automated Data Collection for Single Particle, Auto-data collection for Tomography, JADAS and EMAN 2 with hands on practical experience in the microscopy suite and the computer lab.
Further details and registration forms can be found at http://ncmi.bcm.edu/ncmi/events/workshops/workshops_97. Early registration is essential as places are limited.
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Email: chyun-at-uark.edu Name: Changbae Hyun
Organization: University of Arkansas
Title-Subject: [Filtered] Convert EELS data from text file format to 'Gatan DM imageDocument3í
Question: Is there a way to convert a EELS data from text file to Gatan DM imageDocument3 file format? I have electron counts vs. energy and I want to use Gatan's Digital Micrograph program to analyze EELS data. If anyone knows the way to convert, please let me know.
Thanks, Changbae Hyun PostDoc fellow University of Arkansas 800 W Dickson St, RM226 Fayetteville, AR 72701 e-mail:chyun-at-uark.edu tel:479-575-8764
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I am looking for a Digital Micrograph script file, where we can acquire Multiple EELS spectrum with regular intervals of time. I am interested to study radiation damage by Electron beam on different materials which requires collections of EELS spectra with regular intervals of time.
I truly appreciate your response
Thanks Rao Karavadi Rutgers: The State University of New Jersey 607 Taylor Road Piscataway, NJ 08854 USA
Dale, The standard method for fixing drosophila embryos which have an impermeable cuticle is to use fix with heptane, a partition method. Briefly, put 5ml of your fix and 5ml of heptane in a 20ml vial. Add your sample, screw the cap on very well and agitate vigorously for 20--30 minutes. As the embryos fix they swell and usually break the cuticle. The cuticle free embryos will sink to the bottom in the fix once you stop shaking the vial. Those fixed embryos can be pipetted into another clean vial and fixed longer or rinsed in buffer followed by the standard EM preparation protocols. If this doesn't work--it doesn't work for drosophila larvae, then cool the samples on ice until they stop wiggling and then slice off one end with a razor blade. I like to use a piece of dental wax or polyethylene plastic for the slicing surface. The scoop the samples into fix usually for overnight. Best wishes Larry
dac-at-research.umass.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } I have seen some mentions here of methods to enhance fixation and } embedding of nematodes. I just watched my vinegar eels (T. acetii) swim } happily for } 1hr in the [2% glutaraldehyde + 4% paraformaldhyde + 0.2M } cacodylate, pH 7.4] fixative that was reportedly used in some work with } beautiful results - that work reported a 1hr fixation; surely something } is wrong here. I have seen mention of slicing with a razor blade (can it } really be done? How? These vinegar eels are tiny and very wiggly); } penetrating with a pulled micropipette; cryo fixation; enzymatic } digestion of the cuticle (is this done after fixation?); and finally, } laser beams - what type of laser is required - can this be done with a } confocal or does it need a higher power or other wavelengths? I have } some early/mid-1970's papers that have beautiful ultrastructure with no } special methods mentioned at all - maybe not complete disclosure of the } details? } } I am wondering if electroporation might be any use to allow quicker } permeabilization of a bulk sample. Does anyone know if electroporation } has ever been used - is this a terrible idea? } } I would greatly appreciate any protocols, or references to books or } papers dealing with the nitty-gritty details of such tiny impermeable } preparations. } } Thanks! } } Dale Callaham } } ==============================Original Headers============================== } 6, 20 -- From dac-at-research.umass.edu Fri Jan 23 14:21:30 2009 } 6, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) } 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0NKLUjK004106 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 14:21:30 -0600 } 6, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) } 6, 20 -- (authenticated bits=0) } 6, 20 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0NKLT93017629 } 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 15:21:30 -0500 } 6, 20 -- Message-ID: {497A2712.6060305-at-research.umass.edu} } 6, 20 -- Date: Fri, 23 Jan 2009 15:22:42 -0500 } 6, 20 -- From: Dale Callaham {dac-at-research.umass.edu} } 6, 20 -- Reply-To: dac-at-research.umass.edu } 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 6, 20 -- Subject: Nematode permeabilization for fixation and embedding } 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 6, 20 -- Content-Transfer-Encoding: 7bit } 6, 20 -- X-Whitelist: TRUE } ==============================End of - Headers============================== }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 6, 39 -- From Larry.Ackerman-at-ucsf.edu Fri Jan 23 18:10:37 2009 6, 39 -- Received: from emfmcb02.ucsfmedicalcenter.org (emfmcb02.ucsfmedicalcenter.org [64.54.46.98]) 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0O0Aaw9001773 6, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 23 Jan 2009 18:10:37 -0600 6, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with 6, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2)); 6, 39 -- Fri, 23 Jan 2009 16:10:25 -0800 6, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B 6, 39 -- X-AuditID: 403623d2-ac61fbb000007fb9-63-497a78ab53a6 6, 39 -- Received: from exbhmcb01.ucsfmedicalcenter.org ( 6, 39 -- exbhmcb01.ucsfmedicalcenter.org [64.54.46.222]) by 6, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id 6, 39 -- 4F016129D; Fri, 23 Jan 2009 18:10:51 -0800 (PST) 6, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by 6, 39 -- exbhmcb01.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830); 6, 39 -- Fri, 23 Jan 2009 16:10:24 -0800 6, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 6, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Fri, 23 Jan 6, 39 -- 2009 16:10:24 -0800 6, 39 -- Message-ID: {497A5C6F.5010206-at-ucsf.edu} 6, 39 -- Date: Fri, 23 Jan 2009 16:10:23 -0800 6, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 6, 39 -- Reply-to: larry.ackerman-at-ucsf.edu 6, 39 -- Organization: UCSF, NeuroAnatomy 6, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 6, 39 -- MIME-Version: 1.0 6, 39 -- To: dac-at-research.umass.edu, Microscopy-at-microscopy.com 6, 39 -- Subject: Re: [Microscopy] Nematode permeabilization for fixation and 6, 39 -- embedding 6, 39 -- References: {200901232026.n0NKQfjr018546-at-ns.microscopy.com} 6, 39 -- In-Reply-To: {200901232026.n0NKQfjr018546-at-ns.microscopy.com} 6, 39 -- X-OriginalArrivalTime: 24 Jan 2009 00:10:24.0214 (UTC) 6, 39 -- FILETIME=[2736FF60:01C97DB8] 6, 39 -- X-Brightmail-Tracker: AAAAAQ1Okks= 6, 39 -- X-WSS-ID: 656483FB1SS902737-01-01 6, 39 -- Content-Type: text/plain; 6, 39 -- charset=iso-8859-1; 6, 39 -- format=flowed 6, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are using TAAB LV for several years since Spurr's became unavailble. We had never any problems with staining or infiltraton if propylen oxide is used as intermediate. Only once 2-3 years ago there was a batch that was not possible to cut with glass knives but only with diamond ones. Just another happy customer - no any commercial interest. Both aqueous and alcohol solution of UA work well. Reynolds Pb citrate gives very good contrast.
Sincerely, Alex
-- Dr. Aleksandr Mironov MD, PhD Experimental Officer D.1527, M.Smith Building EM Core Facility, Faculty of Life Sciences University of Manchester Oxford Road Manchester M13 9PT UK
Thank you all very much for so many advices on Histo diamond knife. I must have become one of those people I used to laugh at who live on the old time glory. ;-)
Hong Emory EM (404) 712-8491
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The DVD recordings of the Tutorials presented at M&M 2008 are now available. They are $15.00 plus shipping. I can accept checks, credit cards and institutional PO's. I prefer orders by email or snail mail. Telephone orders are also accepted, but they make me cranky and prone to errors. The full instructions for ordering are at this web address http://microscopy.org/MSAUnits/Education/VideoCatalogue.html.
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The following are the new titles:
307 - Cryo-Fluorescence: A Tool for Correlative Cryo-Light and Cryo-Electron Microscopy, Presented by Cindi L. Schwartz
308 - Live Cell Imaging Limitations. Presented by Simon Watkins
309 - Electron Backscatter Diffraction: Operation and Applications. Presented by: David Field
310 Electron-Probe Microanalysis (EPMA): An Overview for Beginners and a Status Report for Experts. Presented by: Paul Carpenter
311- Lorentz Microscopy -- A Versatile Technique for Studying Magnetic Multilayers, Elements and Nanowires Presented by : John Chapman
312-Stereological Characterization of the Geometry of Three Dimensional Microstructures. Presented by: Robert. T. DeHoff
313 ???Image J, A Useful Tool for Image Processing and Analysis. Presented by : Joel B. Sheffield
314 Job Hunting for Scientific Professionals. Presented by : Bev Maleeff
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Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT DUBLIN
Title-Subject: [Filtered] Looking for carbon
Question: Hello all,
I have been given a sample to analyse. The sample is non conducting and are sputtered with gold. I've run an EDX and found various elements,nothing too interesting, but the spectrum includes carbon. I have explained to the requester that although carbon is present, I cannot discount that the EPMA (Jeol JXA 8600 Superprobe) is depositing carbon during analysis. I note that folk are growing carbon whiskers on AFM tips to produce sharper tips but putting the tip in a SEM for a few minutes; the older the SEM, the better i.e. the SEM deposits carbon..... What is the general opinion regarding detecting carbon using EDX? Appreciated, Emer.
==============================Original Headers============================== 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 2009 10, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QDvfm8009249 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 07:57:42 -0600 10, 11 -- Mime-Version: 1.0 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]} 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600 10, 11 -- To: microscopy-at-microscopy.com 10, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver) 10, 11 -- Subject: viaWWW: Looking for carbon 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
From itas-at-jumpy.it Mon Jan 26 08:27:12 2009 Return-Path: {itas-at-jumpy.it} Received: from google.com ([89.251.42.166]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QERBWK023851 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 26 Jan 2009 08:27:11 -0600 Received: from [101.62.91.135] (HELO google.com) by misty-jakeloo.co.uk; Mon, 26 Jan 2009 15:28:52 +0100 Message-ID: {00000003CE00E892016095919} Reply-To: Tansy Keilbach {1239boler.reene-at-gmail.com}
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: emer.ryan-at-dit.ie [mailto:emer.ryan-at-dit.ie] } Sent: Monday, January 26, 2009 7:59 AM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW: Looking for carbon } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } This Question/Comment was submitted to the Microscopy } Listserver using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } -------------------------------------------------------------- } ------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both emer.ryan-at-dit.ie as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } ------------- } } Email: emer.ryan-at-dit.ie } Name: Emer Ryan } } Organization: CREST DIT DUBLIN } } Title-Subject: [Filtered] Looking for carbon } } Question: Hello all, } } I have been given a sample to analyse. The sample is non } conducting and are sputtered with gold. I've run an EDX and } found various elements,nothing too interesting, but the } spectrum includes carbon. I have explained to the requester } that although carbon is present, I cannot discount that the } EPMA (Jeol JXA 8600 Superprobe) is depositing carbon during } analysis. I note that folk are growing carbon whiskers on AFM } tips to produce sharper tips but putting the tip in a SEM for } a few minutes; the older the SEM, the better i.e. } the SEM deposits carbon..... } What is the general opinion regarding detecting carbon using EDX? } Appreciated, } Emer. } } } } } Login Host: 147.252.66.48 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 } 2009 10, 11 -- Received: from [206.69.208.22] } (msdvpn8.msd.anl.gov [130.202.238.72]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n0QDvfm8009249 } 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan } 2009 07:57:42 -0600 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]} } 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600 10, 11 -- To: } microscopy-at-microscopy.com 10, 11 -- From: emer.ryan-at-dit.ie } (by way of MicroscopyListserver) 10, 11 -- Subject: viaWWW: } Looking for carbon 10, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 25 -- From DusevichV-at-umkc.edu Mon Jan 26 12:24:16 2009 7, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QIOFP0000862 7, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 12:24:15 -0600 7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 25 -- Mon, 26 Jan 2009 12:24:14 -0600 7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 25 -- Content-class: urn:content-classes:message 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="us-ascii" 7, 25 -- Subject: RE: [Microscopy] viaWWW: Looking for carbon 7, 25 -- Date: Mon, 26 Jan 2009 12:23:56 -0600 7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7BE-at-KC-MSX1.kc.umkc.edu} 7, 25 -- In-Reply-To: {200901261358.n0QDweMe010127-at-ns.microscopy.com} 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- Thread-Topic: [Microscopy] viaWWW: Looking for carbon 7, 25 -- thread-index: Acl/vjH5z202usteT22licQ7ReHP+QAJP/Eg 7, 25 -- References: {200901261358.n0QDweMe010127-at-ns.microscopy.com} 7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 25 -- To: {emer.ryan-at-dit.ie} , {Microscopy-at-microscopy.com} 7, 25 -- X-OriginalArrivalTime: 26 Jan 2009 18:24:14.0774 (UTC) FILETIME=[4AE73560:01C97FE3] 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QIOFP0000862 ==============================End of - Headers==============================
Why did you use EDS when you have probe, presumably with WDS? WDS is much better for detection of carbon. If you suspect you detect deposited carbon, you can see (with WDS) if carbon peak is growing with time. Besides, most probes with diffusion pumps are equipped with liquid nitrogen trap. If you have one, it can decrease rate of carbon deposition dramatically.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: emer.ryan-at-dit.ie [mailto:emer.ryan-at-dit.ie] } Sent: Monday, January 26, 2009 7:59 AM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW: Looking for carbon } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } This Question/Comment was submitted to the Microscopy } Listserver using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } -------------------------------------------------------------- } ------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both emer.ryan-at-dit.ie as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } ------------- } } Email: emer.ryan-at-dit.ie } Name: Emer Ryan } } Organization: CREST DIT DUBLIN } } Title-Subject: [Filtered] Looking for carbon } } Question: Hello all, } } I have been given a sample to analyse. The sample is non } conducting and are sputtered with gold. I've run an EDX and } found various elements,nothing too interesting, but the } spectrum includes carbon. I have explained to the requester } that although carbon is present, I cannot discount that the } EPMA (Jeol JXA 8600 Superprobe) is depositing carbon during } analysis. I note that folk are growing carbon whiskers on AFM } tips to produce sharper tips but putting the tip in a SEM for } a few minutes; the older the SEM, the better i.e. } the SEM deposits carbon..... } What is the general opinion regarding detecting carbon using EDX? } Appreciated, } Emer. } } } } } Login Host: 147.252.66.48 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 } 2009 10, 11 -- Received: from [206.69.208.22] } (msdvpn8.msd.anl.gov [130.202.238.72]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n0QDvfm8009249 } 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan } 2009 07:57:42 -0600 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]} } 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600 10, 11 -- To: } microscopy-at-microscopy.com 10, 11 -- From: emer.ryan-at-dit.ie } (by way of MicroscopyListserver) 10, 11 -- Subject: viaWWW: } Looking for carbon 10, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 25 -- From DusevichV-at-umkc.edu Mon Jan 26 12:30:43 2009 8, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QIUg7w006964 8, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 12:30:42 -0600 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 25 -- Mon, 26 Jan 2009 12:30:41 -0600 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="us-ascii" 8, 25 -- Subject: RE: [Microscopy] viaWWW: Looking for carbon 8, 25 -- Date: Mon, 26 Jan 2009 12:30:20 -0600 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7BF-at-KC-MSX1.kc.umkc.edu} 8, 25 -- In-Reply-To: {200901261358.n0QDweMe010127-at-ns.microscopy.com} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: [Microscopy] viaWWW: Looking for carbon 8, 25 -- thread-index: Acl/vjH5z202usteT22licQ7ReHP+QAJd3fw 8, 25 -- References: {200901261358.n0QDweMe010127-at-ns.microscopy.com} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: {emer.ryan-at-dit.ie} , {Microscopy-at-microscopy.com} 8, 25 -- X-OriginalArrivalTime: 26 Jan 2009 18:30:41.0740 (UTC) FILETIME=[318D88C0:01C97FE4] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QIUg7w006964 ==============================End of - Headers==============================
Is the specimen sitting on a Carbon sticky tab? If so, and your beam gets close or on it, you will get C.
C is so ubiquitous that I've rarely seen a specta that does not have some amount of C. Your coater is probably an oil diaphragm pumped system and will back flush some amount of hydrocarbon into the chamber and onto the specimen. Then, taking the specimen out of the coater and exposing it to atmosphere (again) will put more C on it. Then, into the SEM chamber. If roughing pump is oil dual vane mechanical, then likely some back streaming there.
You can try some experiments to baseline your inherent C.
1. Take a clean, unused Al stub and put in SEM. Run at 5KV and collect a spectra. Save it. Then do this at 10KV and save, 15KV and save 20KV and save. At this point, you ought not see polymerization squares/rectangles from the beam.
2. Take another clean, used Al stub and coat it normally. Do the same runs as in #1.
3. Note if you get polymerization patterns.
4. The C from #1 is arguably your background C and can be subtracted from specimen spectra readings. Be sure to collect for the same time, same WD, same DT and same probe conditions. For simple situations, I collect for 60 seconds. For more critical applications, I use 120 seconds. At the standard 10eV per bin, the longer time fills in the peaks and I think improves overall results. Plus it eliminates the collection time variable and standardizes the collection by at least that one variable.
5. Get a stainless set of standards and check them at different KV and see what C you get for each. subtract the background and check correlation for the standard.
C is all over the place so it is hard to make disappear. Based on some communications with an MSA lister, I also noted that O is a joker.
Light element analysis is tricky. Depending on the specimen, I would analyze at 5KV and collect spectra. Then move up the KV. As more of the heavier elements' main peaks show up, the quant will change.
A suggestion. See if it works for you. Sample storage is also a big issue (though it did not occur to me until a couple of years ago, sigh). Store samples under vacuum or purge with N2. A Sample Saver is a great way to do this since you can purge it or pump it with the stubs in place and then close it down. Or you can store specimens in a vacuum oven. Sometimes I don't particularly like the ovens when heated since oxides tend to form immediately upon removal. This is a killer for EBSD.
Let us know how it goes. C is a constant battle for me. Glad I'm not alone. I Probably could write more but then it takes on a short novel!
gary g.
At 05:59 AM 1/26/2009, you wrote:
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Dear Rao, There is a script that will do what you need in the DM Script Database maintained by TU-Graz. The URL is: http://www.felmi-zfe.tugraz.at/dm_scripts/dm_scripts/freeware/programs/Multi ple-EELS-Acquisition.htm
This script was written by David Mitchell. I have not tried it personally, but David's scripts tend to be of the highest quality, work out of the box, and are typically easy to follow and modify if needed. The description of the script follows:
description / instruction This script will capture multiple EELS spectra and store them in a 3D EELS data cube. The EELS data cube is then processed to sum the spectra. Alignment and summation of spectra can be done with or without energy shift correction (to compensate for any energy drift). EELS data cubes can also be processed offline using the script 'EELS Cube Data Extraction', available at this site. Offline processing permits individual spectra to be extracted and spectra to be omitted from the summation. To use this script set up the spectrometer to display an EELS spectrum on the CCD - then run the script - enter exposure time, number of frames and any delay between frame capture. To remove X-ray spikes from the data, use the script 'EELS Cube Data Editor'.
The URL for the TU-Graz database is: http://www.felmi-zfe.tugraz.at/dm_scripts/welcome.html
This database is not supported or maintained by Gatan. It is provided as a community service by the kind folks at FELMI-ZFE
Best regards, Ray
Ray D. Twesten, Ph.D. Product Manager – Analytical Instruments Gatan, Inc. Tel. +1 (925) 224-7392
-----Original Message----- X-from: rao.karavadi-at-rutgers.edu [mailto:rao.karavadi-at-rutgers.edu] Sent: Friday, January 23, 2009 3:32 PM To: ray.twesten-at-sbcglobal.net
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I am looking for a Digital Micrograph script file, where we can acquire Multiple EELS spectrum with regular intervals of time. I am interested to study radiation damage by Electron beam on different materials which requires collections of EELS spectra with regular intervals of time.
I truly appreciate your response
Thanks Rao Karavadi Rutgers: The State University of New Jersey 607 Taylor Road Piscataway, NJ 08854 USA
I would run comparative samples to verify or disprove your findings. I would try to find samples as similar in nature as possible, but even if you can't match textures you could compare the chemistry.
You could examine graphite as one extreme, polymers such as polyethylene or polystyrene, and glass or metals as the other extreme.
The peak-to-background ratios are important. I would encourage scaling the spectra to match the background intensities. You could use the spectrum from graphite to see what 100% carbon should look like. The polyethylene might show a little less carbon since it has hydrogen which you cannot see. Glass or pure metals should not have any carbon, so the only carbon you see there should be that due to contamination build up. And build-up will grow with time. You should be able to compare successive spectra and see growth in the carbon peak.
I would offer those results to your client and see what most resembles their sample. If they have only low levels of carbon in their spectra, it may be hard to say much about the source of that carbon. Maybe it was really present. Maybe it was contamination from the scope. Maybe it was contamination from the sample. Give it a try and see what happens.
Warren S.
-----Original Message----- X-from: emer.ryan-at-dit.ie [mailto:emer.ryan-at-dit.ie] Sent: Monday, January 26, 2009 7:59 AM To: wesaia-at-iastate.edu
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Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT DUBLIN
Title-Subject: [Filtered] Looking for carbon
Question: Hello all,
I have been given a sample to analyse. The sample is non conducting and are sputtered with gold. I've run an EDX and found various elements,nothing too interesting, but the spectrum includes carbon. I have explained to the requester that although carbon is present, I cannot discount that the EPMA (Jeol JXA 8600 Superprobe) is depositing carbon during analysis. I note that folk are growing carbon whiskers on AFM tips to produce sharper tips but putting the tip in a SEM for a few minutes; the older the SEM, the better i.e. the SEM deposits carbon..... What is the general opinion regarding detecting carbon using EDX? Appreciated, Emer.
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------------------------------------------------------------------------------------ Don Becker U.S. Sales Manager - Microanalysis Bruker AXS Inc. 1239 Parkway Avenue, Suite 203 Ewing, NJ 08628 Tel: +1 (609) 771-4400 Fax: +1 (609) 771-4411 don.becker-at-bruker-axs.com www.bruker-axs.com ------------------------------------------------------------------------------------
==============================Original Headers============================== 11, 28 -- From Don.Becker-at-bruker-axs.com Mon Jan 26 15:19:11 2009 11, 28 -- Received: from mail1.bruker-axs.com (mail2.bruker-axs.com [12.18.13.126]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QLJAen017452 11, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 15:19:11 -0600 11, 28 -- Received: from mail1.bruker-axs.com ([172.16.0.32]) by mail1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.3959); 11, 28 -- Mon, 26 Jan 2009 15:19:29 -0600 11, 28 -- X-Ninja-PIM: Scanned by Ninja 11, 28 -- X-Ninja-AttachmentFiltering: (no action) 11, 28 -- Received: from msnmail1.bruker-axs.com ([172.16.0.53]) by mail1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.3959); 11, 28 -- Mon, 26 Jan 2009 15:19:07 -0600 11, 28 -- Received: from msnmail1.bruker-axs.com ([172.16.0.53]) by 11, 28 -- msnmail1.bruker-axs.com ([172.16.0.53]) with mapi; Mon, 26 Jan 2009 15:19:06 11, 28 -- -0600 11, 28 -- From: "Becker, Don" {Don.Becker-at-bruker-axs.com} 11, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 28 -- Date: Mon, 26 Jan 2009 15:19:05 -0600 11, 28 -- Subject: Immediate Sales Opening at Bruker AXS Microanalysis 11, 28 -- Thread-Topic: Immediate Sales Opening at Bruker AXS Microanalysis 11, 28 -- Thread-Index: Acl/+7glyZxdzV9LSri0uywtf0YPAA== 11, 28 -- Message-ID: {6CBB09E530A398488E07976781F692030FB0A14909-at-msnmail1.bruker-axs.com} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-OriginalArrivalTime: 26 Jan 2009 21:19:07.0254 (UTC) FILETIME=[B8E89960:01C97FFB] 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0QLJAen017452 ==============================End of - Headers==============================
************************************************* * MICROBEAM ANALYSIS SOCIETY * * * * Microanalysis of Particles 2009 * * Topical Conference * * * * April 21-23, 2009 * * Westmont, Il * * * ************************************************
This conference takes a pragmatic approach to a subtle subject - the microanalysis of particles. Techniques covered include EDS, WDS, AEM, SIMS, ESCA, CL, XRD, synchrotron XRF. We have invited some of the most well known names in the industry to present extended talks in the morning. In the afternoon, attendees will be able to select among various hands-on modules. Both novices and experienced analysts will benefit.
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==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Mon Jan 26 17:05:48 2009 7, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0QN5lAg019325 7, 20 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 17:05:47 -0600 7, 20 -- Message-Id: {200901262305.n0QN5lAg019325-at-ns.microscopy.com} 7, 20 -- Received: (qmail 28562 invoked from network); 26 Jan 2009 15:00:51 -0800 7, 20 -- Received: by simscan 1.1.0 ppid: 28555, pid: 28556, t: 0.1671s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by smtp1 with SMTP; 26 Jan 2009 15:00:51 -0800 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Mon, 26 Jan 2009 15:05:44 -0800 7, 20 -- To: gary-at-gaugler.com 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] Re: viaWWW: Looking for carbon 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200901261945.n0QJjcZB001291-at-ns.microscopy.com} 7, 20 -- References: {200901261945.n0QJjcZB001291-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Email: y.han-at-sheffield.ac.uk Name: Yisong Han
Organization: University of Sheffield
Title-Subject: [Filtered] preparation of plan-view TEM samples
Question: Dear All,
Does anybody know how to protect plan-view TEM samples, which are thinned from one side only, from contamination during ion beam milling? Thanks very much in advance.
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Email: aochalsk-at-uottawa.ca Name: Andrew Ochalski
Organization: University of Ottawa
Title-Subject: [Filtered] Hardware/Software for Olympus Fluoview FVX confocal
Question: Hi all,
Four years ago, we were given an older confocal microscope for educational purposes, specifically an Olympus Fluoview FVX. The software, Fluoview 2.1 as well as the scan/stage controller and scan head all work together in a Win NT environment. We have none of the original software disks and the (older) PC on which the whole lot is running is constantly crashing (blue screen of death)and is probably not long for this world. Olympus used to sell an upgrade package compatible with WinXP, but Olympus is out of them and they are not being made any more. If anyone has an upgraded system that they are no longer using or, for any reason have a version of the controller, card and updated software that they would consider selling (or giving away), we would be thrilled to hear from you.
Andrew Ochalski, Technical Supervisor, Carsen Advanced Educational Microscopy Resource Centre Biology Department University of Ottawa 30 Marie Curie Ottawa, ON CANADA K1N 6N5
Evaporate salt (NaCl) on the side you want to protect. Dissolve the salt in water when you are finished ion milling and the contamination will flush away.
Ron Anderson
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==============================Original Headers============================== 4, 19 -- From randerson20-at-tampabay.rr.com Tue Jan 27 16:26:54 2009 4, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RMQr5q014197 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 27 Jan 2009 16:26:54 -0600 4, 19 -- Received: from [127.0.0.1] (really [24.73.73.214]) 4, 19 -- by hrndva-omta06.mail.rr.com with ESMTP 4, 19 -- id {20090127222653.TIOS10307.hrndva-omta06.mail.rr.com-at-[127.0.0.1]} ; 4, 19 -- Tue, 27 Jan 2009 22:26:53 +0000 4, 19 -- Message-ID: {497F8A24.9020105-at-tampabay.rr.com} 4, 19 -- Date: Tue, 27 Jan 2009 17:26:44 -0500 4, 19 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 4, 19 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 4, 19 -- MIME-Version: 1.0 4, 19 -- To: y.han-at-sheffield.ac.uk, Listserver {Microscopy-at-Microscopy.Com} 4, 19 -- Subject: Re: [Microscopy] viaWWW: preparation of plan-view TEM samples 4, 19 -- References: {200901272149.n0RLnXJs018374-at-ns.microscopy.com} 4, 19 -- In-Reply-To: {200901272149.n0RLnXJs018374-at-ns.microscopy.com} 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Email: jsiegmund-at-7thwavelabs.com Name: Joachim Siegmund Organization: SeventhWave Labs
On the software side, ImageJ with the UCSD plugin might work for you: http://rsb.info.nih.gov/ij/plugins/ucsd.html
On the driver side, you can try whether or not the win2000 drivers work with winXP ... sometimes they do. There is also a compatibility mode in XP for old software, I think.
Joachim
-----Original Message----- X-from: aochalsk-at-uottawa.ca [mailto:aochalsk-at-uottawa.ca] Sent: Tuesday, January 27, 2009 4:02 PM To: Joachim Siegmund
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Email: aochalsk-at-uottawa.ca Name: Andrew Ochalski
Organization: University of Ottawa
Title-Subject: [Filtered] Hardware/Software for Olympus Fluoview FVX confocal
Question: Hi all,
Four years ago, we were given an older confocal microscope for educational purposes, specifically an Olympus Fluoview FVX. The software, Fluoview 2.1 as well as the scan/stage controller and scan head all work together in a Win NT environment. We have none of the original software disks and the (older) PC on which the whole lot is running is constantly crashing (blue screen of death)and is probably not long for this world. Olympus used to sell an upgrade package compatible with WinXP, but Olympus is out of them and they are not being made any more. If anyone has an upgraded system that they are no longer using or, for any reason have a version of the controller, card and updated software that they would consider selling (or giving away), we would be thrilled to hear from you.
Andrew Ochalski, Technical Supervisor, Carsen Advanced Educational Microscopy Resource Centre Biology Department University of Ottawa 30 Marie Curie Ottawa, ON CANADA K1N 6N5
==============================Original Headers============================== 10, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:53 2009 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnq5V018607 10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:53 -0600 10, 11 -- Mime-Version: 1.0 10, 11 -- Message-Id: {p06240801c5a531eb8978-at-[206.69.208.22]} 10, 11 -- Date: Tue, 27 Jan 2009 15:49:52 -0600 10, 11 -- To: microscopy-at-microscopy.com 10, 11 -- From: aochalsk-at-uottawa.ca (by way of MicroscopyListserver) 10, 11 -- Subject: viaWWW: Hardware/Software for Olympus Fluoview FVX confocal 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 29 -- From jsiegmund-at-7thwavelabs.com Tue Jan 27 16:52:57 2009 22, 29 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136]) 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RMqvJ3028688 22, 29 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 16:52:57 -0600 22, 29 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1]) 22, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id EF4AD39800A; 22, 29 -- Tue, 27 Jan 2009 16:52:56 -0600 (CST) 22, 29 -- X-AuditID: c0a80218-a6841bb000000bbe-63-497f9048ba21 22, 29 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29]) 22, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 86013424013; 22, 29 -- Tue, 27 Jan 2009 16:52:56 -0600 (CST) 22, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 29 -- Content-class: urn:content-classes:message 22, 29 -- MIME-Version: 1.0 22, 29 -- Content-Type: text/plain; 22, 29 -- charset="us-ascii" 22, 29 -- Subject: RE: [Microscopy] viaWWW: Hardware/Software for Olympus Fluoview FVX confocal 22, 29 -- Date: Tue, 27 Jan 2009 16:52:56 -0600 22, 29 -- Message-ID: {62A8156F8071C8439080D626DF8C33A660AC86-at-wave-mail.7thwave.local} 22, 29 -- X-MS-Has-Attach: 22, 29 -- X-MS-TNEF-Correlator: 22, 29 -- Thread-Topic: [Microscopy] viaWWW: Hardware/Software for Olympus Fluoview FVX confocal 22, 29 -- Thread-Index: AcmAyvBuSyjctnk4REaRT06YHHto6QABbsAg 22, 29 -- References: {200901272202.n0RM2O5x012850-at-ns.microscopy.com} 22, 29 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com} 22, 29 -- To: {aochalsk-at-uottawa.ca} , {microscopy-at-microscopy.com} 22, 29 -- X-Brightmail-Tracker: AAAAAQ1k0lE= 22, 29 -- Content-Transfer-Encoding: 8bit 22, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0RMqvJ3028688 ==============================End of - Headers==============================
1. Paint the side you want to protect with a layer of clear fingernail polish and dissolve in acetone after ion milling is finished.
2. I used to cut out a 3mm disk of a very thin sheet of mica and place that under the specimen. Any redeposited material will stick to the mica disk.
Craig.
------------------------------- Craig L. Johnson TEM Scientist Centralized Research Facility Drexel University College of Engineering -------------------------------
} } On Tue, Jan 27, 2009 at 5:31 PM, {randerson20-at-tampabay.rr.com} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Evaporate salt (NaCl) on the side you want to protect. Dissolve the salt } } in water when you are finished ion milling and the contamination will } } flush away. } } } } Ron Anderson } } } } y.han-at-sheffield.ac.uk wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at } } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so when replying } } } please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver } } } --------------------------------------------------------------------------- } } } } } } Email: y.han-at-sheffield.ac.uk } } } Name: Yisong Han } } } } } } Organization: University of Sheffield } } } } } } Title-Subject: [Filtered] preparation of plan-view TEM samples } } } } } } Question: Dear All, } } } } } } Does anybody know how to protect plan-view TEM samples, which are } } } thinned from one side only, from contamination during ion beam } } } milling? Thanks very much in advance. } } } } } } Yisong } } } } } } Login Host: 143.167.204.169 } } } --------------------------------------------------------------------------- } } } } } } ==============================Original Headers============================== } } } 8, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:22 2009 } } } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnLba018216 } } } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:22 -0600 } } } 8, 11 -- Mime-Version: 1.0 } } } 8, 11 -- Message-Id: {p06240800c5a531d0832d-at-[206.69.208.22]} } } } 8, 11 -- Date: Tue, 27 Jan 2009 15:49:20 -0600 } } } 8, 11 -- To: microscopy-at-microscopy.com } } } 8, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver) } } } 8, 11 -- Subject: viaWWW: preparation of plan-view TEM samples } } } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } } ==============================End of - Headers============================== } } } } } } } } } } } } } } } ==============================Original Headers============================== } } 4, 19 -- From randerson20-at-tampabay.rr.com Tue Jan 27 16:26:54 2009 } } 4, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) } } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RMQr5q014197 } } 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 27 Jan 2009 16:26:54 -0600 } } 4, 19 -- Received: from [127.0.0.1] (really [24.73.73.214]) } } 4, 19 -- by hrndva-omta06.mail.rr.com with ESMTP } } 4, 19 -- id {20090127222653.TIOS10307.hrndva-omta06.mail.rr.com-at-[127.0.0.1]} ; } } 4, 19 -- Tue, 27 Jan 2009 22:26:53 +0000 } } 4, 19 -- Message-ID: {497F8A24.9020105-at-tampabay.rr.com} } } 4, 19 -- Date: Tue, 27 Jan 2009 17:26:44 -0500 } } 4, 19 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} } } 4, 19 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) } } 4, 19 -- MIME-Version: 1.0 } } 4, 19 -- To: y.han-at-sheffield.ac.uk, Listserver {Microscopy-at-Microscopy.Com} } } 4, 19 -- Subject: Re: [Microscopy] viaWWW: preparation of plan-view TEM samples } } 4, 19 -- References: {200901272149.n0RLnXJs018374-at-ns.microscopy.com} } } 4, 19 -- In-Reply-To: {200901272149.n0RLnXJs018374-at-ns.microscopy.com} } } 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 4, 19 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 6, 35 -- From cljohnson33-at-gmail.com Tue Jan 27 17:23:42 2009 6, 35 -- Received: from yx-out-1718.google.com (yx-out-1718.google.com [74.125.44.153]) 6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RNNfZJ010259 6, 35 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 17:23:42 -0600 6, 35 -- Received: by yx-out-1718.google.com with SMTP id 36so2657613yxh.0 6, 35 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:23:41 -0800 (PST) 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 35 -- d=gmail.com; s=gamma; 6, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references 6, 35 -- :date:message-id:subject:from:to:content-type 6, 35 -- :content-transfer-encoding; 6, 35 -- bh=6VxQLoCgeWnQS8yLUFCYyTw7jp0qu6v+g0cCcnOLvA0=; 6, 35 -- b=k9rnm3IgtR60LMtKTyTljOQHdKV3K0xJsBwn0NswiGFfOS6+fN500bewkQpY3AWWGK 6, 35 -- +PZQIURVw8oS276GHrNyojShHZANz8w+T7VFUqZKcMKj0We9AyqWby1DcI7abGmA2FOo 6, 35 -- z3+TcrZFON497Alyh8UyAnN/xb1DG3Dp4e0g0= 6, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 35 -- d=gmail.com; s=gamma; 6, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 6, 35 -- :content-type:content-transfer-encoding; 6, 35 -- b=npdd4MHtTjbBlBHfN/GbYFG2AvOLID1KX44HgAsWSh0me/wy7fT4OOiUSOZxiM2G+1 6, 35 -- uCWLbxFS1e7mPq+CuhNOEMmU4Y3uf3fTe7Ii56sKmHKO8OP13Jx5DEJ4ZwPIwn2aNy2x 6, 35 -- y9WzcFo5mGsmWWnui1GPLx7+nLfse8VKvUaNQ= 6, 35 -- MIME-Version: 1.0 6, 35 -- Received: by 10.150.11.14 with SMTP id 14mr347575ybk.101.1233098621512; Tue, 6, 35 -- 27 Jan 2009 15:23:41 -0800 (PST) 6, 35 -- In-Reply-To: {611c9b6f0901271522o7998ab43p2aed2e4e16cd429a-at-mail.gmail.com} 6, 35 -- References: {200901272231.n0RMVQkI024095-at-ns.microscopy.com} 6, 35 -- {611c9b6f0901271522o7998ab43p2aed2e4e16cd429a-at-mail.gmail.com} 6, 35 -- Date: Tue, 27 Jan 2009 18:23:41 -0500 6, 35 -- Message-ID: {611c9b6f0901271523t5b603428iad45ff9f8cc0e361-at-mail.gmail.com} 6, 35 -- Subject: Re: [Microscopy] Re: viaWWW: preparation of plan-view TEM samples 6, 35 -- From: Craig Johnson {cljohnson33-at-gmail.com} 6, 35 -- To: microscopy-at-microscopy.com 6, 35 -- Content-Type: text/plain; charset=ISO-8859-1 6, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Just to add to Ron Anderson's and Craig Johnson's posts - if you use glycopthalate wax (which is often sold under a trade name of QuickStick or something like that), you can put a small amount into a few ml of acetone and paint it on the surface you want to protect. The wax is totally soluble in acetone and should leave no residue (okay, I guess a small amount of amorphous carbon on the atomic scale). I'm not sure that nail varnish would do the same.
Richard Beanland
y.han-at-sheffield.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: y.han-at-sheffield.ac.uk } Name: Yisong Han } } Organization: University of Sheffield } } Title-Subject: [Filtered] preparation of plan-view TEM samples } } Question: Dear All, } } Does anybody know how to protect plan-view TEM samples, which are } thinned from one side only, from contamination during ion beam } milling? Thanks very much in advance. } } Yisong } } Login Host: 143.167.204.169 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Jan 27 15:49:22 2009 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0RLnLba018216 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 Jan 2009 15:49:22 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c5a531d0832d-at-[206.69.208.22]} } 8, 11 -- Date: Tue, 27 Jan 2009 15:49:20 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: preparation of plan-view TEM samples } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 4, 29 -- From contact-at-integrityscientific.com Wed Jan 28 03:47:07 2009 4, 29 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.csv.warwick.ac.uk [137.205.128.7]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0S9l7Y4015263 4, 29 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 03:47:07 -0600 4, 29 -- Received: from localhost (localhost [127.0.0.1]) 4, 29 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n0S9l48e017243; 4, 29 -- Wed, 28 Jan 2009 09:47:04 GMT 4, 29 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 4, 29 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 4, 29 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 4, 29 -- with LMTP id Y+Lij45W7ERS; Wed, 28 Jan 2009 09:46:59 +0000 (GMT) 4, 29 -- Received: from [137.205.164.101] (hosts-137-205-164-101 [137.205.164.101]) 4, 29 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n0S9kies017068; 4, 29 -- Wed, 28 Jan 2009 09:46:44 GMT 4, 29 -- X-Envelope-From: contact-at-integrityscientific.com 4, 29 -- Message-ID: {4980297E.2010504-at-integrityscientific.com} 4, 29 -- Date: Wed, 28 Jan 2009 09:46:38 +0000 4, 29 -- From: Richard Beanland {contact-at-integrityscientific.com} 4, 29 -- Reply-To: contact-at-integrityscientific.com 4, 29 -- Organization: Integrity Scientific Ltd 4, 29 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 4, 29 -- MIME-Version: 1.0 4, 29 -- To: y.han-at-sheffield.ac.uk, microscopy-at-microscopy.com 4, 29 -- Subject: Re: [Microscopy] viaWWW: preparation of plan-view TEM samples 4, 29 -- References: {200901272159.n0RLxEV4005110-at-ns.microscopy.com} 4, 29 -- In-Reply-To: {200901272159.n0RLxEV4005110-at-ns.microscopy.com} 4, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- X-DCC-Warwick-Metrics: bluebell; whitelist ==============================End of - Headers==============================
You wrote "The sample is non conducting and are sputtered with gold. ". Depending of how thick and how grainy your gold coating is, the C signal you see will only partially reflect the C present in the sample. Absorbtion of the carbon line by the gold layer may be strong. A little peak could reflect a low surface contamiantion as welle as a high carbon presence in the sample.
An other way to test your sample, would be to do EDS at low voltage (less then 5 kev, better 3 keV) without coating. It is possible (more or less easy) to find conditions where one have no or low charging, without coating. At low energy, one have too a much better emission yield on low energy x-ray lines.
If contamination occurs, you should see it well at low voltage too, and better with an in lens SE detector (of coarse not on the JXA).
As other have said, low energy lines are difficulte, C and N in particular (but Be or B too !).
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
emer.ryan-at-dit.ie a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: emer.ryan-at-dit.ie } Name: Emer Ryan } } Organization: CREST DIT DUBLIN } } Title-Subject: [Filtered] Looking for carbon } } Question: Hello all, } } I have been given a sample to analyse. The sample is non conducting } and are sputtered with gold. I've run an EDX and found various } elements,nothing too interesting, but the spectrum includes carbon. I } have explained to the requester that although carbon is present, I } cannot discount that the EPMA (Jeol JXA 8600 Superprobe) is } depositing carbon during analysis. I note that folk are growing } carbon whiskers on AFM tips to produce sharper tips but putting the } tip in a SEM for a few minutes; the older the SEM, the better i.e. } the SEM deposits carbon..... } What is the general opinion regarding detecting carbon using EDX? } Appreciated, } Emer. } } } } } Login Host: 147.252.66.48 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 11 -- From zaluzec-at-microscopy.com Mon Jan 26 07:57:43 2009 } 10, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0QDvfm8009249 } 10, 11 -- for {microscopy-at-microscopy.com} ; Mon, 26 Jan 2009 07:57:42 -0600 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240801c5a3719b126d-at-[206.69.208.22]} } 10, 11 -- Date: Mon, 26 Jan 2009 07:57:40 -0600 } 10, 11 -- To: microscopy-at-microscopy.com } 10, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver) } 10, 11 -- Subject: viaWWW: Looking for carbon } 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 11, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Jan 28 06:44:33 2009 11, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156]) 11, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SCiWJd002805 11, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 06:44:33 -0600 11, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 11, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n0SCiRAt016098 11, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 13:44:27 +0100 (CET) 11, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 11, 32 -- (No client certificate requested) 11, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 378263EC005 11, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 28 Jan 2009 13:44:15 +0100 (CET) 11, 32 -- Message-ID: {4980531E.4010005-at-ipcms.u-strasbg.fr} 11, 32 -- Date: Wed, 28 Jan 2009 13:44:14 +0100 11, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 32 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 11, 32 -- MIME-Version: 1.0 11, 32 -- To: Microscopy-at-microscopy.com 11, 32 -- Subject: Re: [Microscopy] viaWWW: Looking for carbon 11, 32 -- References: {200901261404.n0QE4e50017078-at-ns.microscopy.com} 11, 32 -- In-Reply-To: {200901261404.n0QE4e50017078-at-ns.microscopy.com} 11, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 32 -- Content-Transfer-Encoding: 8bit 11, 32 -- X-IPCMS-MailScanner: Found to be clean 11, 32 -- X-IPCMS-MailScanner-SpamScore: s 11, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 11, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.156]); Wed, 28 Jan 2009 13:44:27 +0100 (CET) 11, 32 -- X-Virus-Scanned: ClamAV 0.94.2/8914/Wed Jan 28 07:40:00 2009 on mr6.u-strasbg.fr 11, 32 -- X-Virus-Status: Clean 11, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST 11, 32 -- autolearn=disabled version=3.2.5 11, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr6.u-strasbg.fr ==============================End of - Headers==============================
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Email: gary-at-perfendo.com Name: Gary B. Carr
Organization: Pacific Endodontic Research Foundation
Title-Subject: [Filtered] book request
Question: Does anyone have a copy of: Biomedical Electron Microscopy: Illustrated Methods and Interpretations by Maunsbach that they would like to sell me?
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Email: jrcastel-at-quim.ucm.es Name: Julio Ramirez-Castellanos
Organization: Complutense Univ. Madrid
Title-Subject: [Filtered] Sample sensitivity under de e-beam
Question: I am trying to examine a GeO2 sample by HRTEM, however, the sample is very unstable under de electron beam .... what can I do .... thanks.
There was a recent string on the listserv concerning substitutes for the late, lamented Spurr's resin. Really? Is Spurr's gone? We just ordered some from our usual suppliers and I haven't heard anything about its being discontinued. Have I missed something (not impossible or even unlikely some days)?
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 6, 27 -- From TindallR-at-missouri.edu Wed Jan 28 09:42:27 2009 6, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SFgRA9017634 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 09:42:27 -0600 6, 27 -- X-IronPort-Anti-Spam-Filtered: true 6, 27 -- X-IronPort-Anti-Spam-Result: ApoEANQLgEnRauUo/2dsb2JhbAC+SgEJhTyIR4QagS0G 6, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 6, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 28 Jan 2009 09:42:27 -0600 6, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Wed, 28 Jan 2009 09:42:27 -0600 6, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="us-ascii" 6, 27 -- Subject: Demise of Spurr's? 6, 27 -- Date: Wed, 28 Jan 2009 09:42:26 -0600 6, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7D3D-at-UM-XMAIL08.um.umsystem.edu} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Demise of Spurr's? 6, 27 -- Thread-Index: AcmBXwTN8cOZv1rDTk25PFQ4yTYXJg== 6, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 28 Jan 2009 15:42:27.0274 (UTC) FILETIME=[059BC2A0:01C9815F] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SFgRA9017634 ==============================End of - Headers==============================
Kraft Foods, Global, Inc. is currently seeking a Scientist/Microscopist to work in our R&D facility in Glenview,IL.
The candidate will thrive on the opportunity to resolve problems/build knowledge in the Food R&D arena by the application of advanced microscopy. The candidate will work in a team with other structural scientists (light microscopy, Confocal, SEM,TEM) and chemical and physical scientists.
Utilize expertise in spatial resolution ranging from light microscopy to electron microscopy combined with spectral imaging in order to resolve problems and build knowledge in Food/Packaging. PhD or equivalent in biology/biochemicstry with a proficiency in advanced microscopy techniques including Energy Filtering TEM, SEM x-ray microanalysis, and histochemistry are expected along with both solo and team work capablities. The position requires the ability to work on several projects simultaneously and communicate finding to other scientists, product developers and managers.
PhD in Bio/Chem or Equivalent Minimum 1-3 years of experience in Biology/Life Sciences Minimum 1-3 years of experience in Chemistry High skill level in EFTEM & SEM Experience in EM sample preparation methods for diverse foods Computer skills in image analysis and digital imaging Food Sciences
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Kraft Foods offers a competitive compensation and benefits package including health care coverage, generous 401k match, annual incentive bonus and paid time off.
Candidates who are interested in applying for this position should do so via the Kraft Foods applicant tracking system, by cutting and pasting the following link into their web browser:
Matt LaFramboise Kraft Foods R&D Recruiter 847.646.9241 Matt.laframboise-at-kraft.com
==============================Original Headers============================== 11, 26 -- From Matt.Laframboise-at-Kraft.com Wed Jan 28 10:06:33 2009 11, 26 -- Received: from mail5.kraft.com (mail5.kraft.com [206.228.222.60]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SG6VPE032117 11, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:06:32 -0600 11, 26 -- X-IronPort-AV: E=McAfee;i="5300,2777,5508"; a="95208484" 11, 26 -- Received: from unknown (HELO kftusoktulxbh03.KRFT.Net) ([10.53.184.16]) 11, 26 -- by mail5.kraft.com with ESMTP; 28 Jan 2009 11:02:36 -0500 11, 26 -- Received: from KFTUSPAWBAXMB31.KRFT.Net ([10.53.184.64]) by kftusoktulxbh03.KRFT.Net with Microsoft SMTPSVC(6.0.3790.3959); 11, 26 -- Wed, 28 Jan 2009 10:02:36 -0600 11, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 26 -- Content-class: urn:content-classes:message 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Type: text/plain; 11, 26 -- charset="us-ascii" 11, 26 -- Subject: Job Opportunity: Scientist/Microscopist 11, 26 -- Date: Wed, 28 Jan 2009 11:02:35 -0500 11, 26 -- Message-ID: {0A7A37452676694E97DBE0232CF6FA3A4DE425-at-KFTUSPAWBAXMB31.KRFT.Net} 11, 26 -- X-MS-Has-Attach: 11, 26 -- X-MS-TNEF-Correlator: 11, 26 -- Thread-Topic: Job Opportunity: Scientist/Microscopist 11, 26 -- Thread-Index: AcmBYdWz/H5+Ay93SNm8OLAmrxTSVA== 11, 26 -- From: {Matt.Laframboise-at-Kraft.com} 11, 26 -- To: {Microscopy-at-microscopy.com} 11, 26 -- X-OriginalArrivalTime: 28 Jan 2009 16:02:36.0271 (UTC) FILETIME=[D63A0FF0:01C98161] 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SG6VPE032117 ==============================End of - Headers==============================
In accordance with "List" protocol, I am copying this to the List. I sent it to Randy offline with some attachments that can't go to the list. The essential attachment, the new formulation is included at the bottom of this message.
Dale -----------------------
Hi Randy,
I think I was responsible for starting the most recent thread on Spurr's resin. At the time I had bought a Spurr's Kit from EMS and had problems with it. I noticed that one of the components had been changed and was far more viscous than the original component. I checked the included docs and it gave the same mixture as the original and made no mention of the viscosity - that was from the bottle. ------------------------------------------------ Note that one of the components of the Spurr's resin (VCD = ERL-4206) is no longer made and is replaced by a new component (ERL-4221) that is much more viscous and has a different WPE value. E. Ann Ellis has published new formulations to use with the new resin components. --------------------------------------------------- Several people told me of Ann Ellis' work/publications on this, and the corrected formulation. See attached documents. ---------------------------- Corrected Formulation for Spurr Low Viscosity Embedding Medium Using the Replacement Epoxide ERL 4221. E. Ann Ellis Microsc Microanal 12(Supp 2), 2006 Microscopy and Imaging Center, BSBW 119/MS 2257, Texas A&M University, College Station, TX 77843-2257 ------------------------------- E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006 Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations" ---------------------------------------------
Eventually Stacie at EMS chimed in and said they had been aware of it and she produced some data.. that had not been present in the catalog, nor included with the kits they sold as Spurr's resin.....
Hope this helps.
Dale
------------------------------------------------ Spurr-replacement "New Formulation"
E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006 Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations"
All formulas are for a "10g batch" - with reference to (epoxy + acid anhydride)
4.10 5.90 0.95 0.10 Hard 4.10 5.90 1.43 0.10 Standard 4.10 5.90 1.90 0.10 Soft
Low Viscosity Formula (Half the viscosity of the "Refomulated Spurr's") ------------------------- ERL 4221 2.22g Quetol-651 1.40g NSA 6.38g DER-736 1.43g BDMA 0.2g Some think BDMA should be less, 0.1g
I (DAC) used 0.09g DMAE (5 drops from a Samco #241 pipet) and it set nicely in 16h -at- 70C
TindallR-at-missouri.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } There was a recent string on the listserv concerning substitutes for the } late, lamented Spurr's resin. Really? Is Spurr's gone? We just } ordered some from our usual suppliers and I haven't heard anything about } its being discontinued. Have I missed something (not impossible or } even unlikely some days)? } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } } ==============================Original Headers============================== } 6, 27 -- From TindallR-at-missouri.edu Wed Jan 28 09:42:27 2009 } 6, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SFgRA9017634 } 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 09:42:27 -0600 } 6, 27 -- X-IronPort-Anti-Spam-Filtered: true } 6, 27 -- X-IronPort-Anti-Spam-Result: ApoEANQLgEnRauUo/2dsb2JhbAC+SgEJhTyIR4QagS0G } 6, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) } 6, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 28 Jan 2009 09:42:27 -0600 } 6, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 6, 27 -- Wed, 28 Jan 2009 09:42:27 -0600 } 6, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 6, 27 -- Content-class: urn:content-classes:message } 6, 27 -- MIME-Version: 1.0 } 6, 27 -- Content-Type: text/plain; } 6, 27 -- charset="us-ascii" } 6, 27 -- Subject: Demise of Spurr's? } 6, 27 -- Date: Wed, 28 Jan 2009 09:42:26 -0600 } 6, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7D3D-at-UM-XMAIL08.um.umsystem.edu} } 6, 27 -- X-MS-Has-Attach: } 6, 27 -- X-MS-TNEF-Correlator: } 6, 27 -- Thread-Topic: Demise of Spurr's? } 6, 27 -- Thread-Index: AcmBXwTN8cOZv1rDTk25PFQ4yTYXJg== } 6, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 6, 27 -- To: {microscopy-at-microscopy.com} } 6, 27 -- X-OriginalArrivalTime: 28 Jan 2009 15:42:27.0274 (UTC) FILETIME=[059BC2A0:01C9815F] } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n0SFgRA9017634 } ==============================End of - Headers==============================
==============================Original Headers============================== 20, 20 -- From dac-at-research.umass.edu Wed Jan 28 10:45:12 2009 20, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SGjABR015008 20, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:45:11 -0600 20, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged)) 20, 20 -- (authenticated bits=0) 20, 20 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0SGj8Gh003537 20, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 20, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 11:45:08 -0500 20, 20 -- Message-ID: {49808BD1.20005-at-research.umass.edu} 20, 20 -- Date: Wed, 28 Jan 2009 11:46:09 -0500 20, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 20, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 20, 20 -- MIME-Version: 1.0 20, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 20, 20 -- Subject: Re: [Microscopy] Demise of Spurr's? 20, 20 -- References: {200901281548.n0SFmUpb025875-at-ns.microscopy.com} 20, 20 -- In-Reply-To: {200901281548.n0SFmUpb025875-at-ns.microscopy.com} 20, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 20, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
--On 28 January 2009 09:53 -0600 TindallR-at-missouri.edu wrote: } ------------------------------------------------------------------------- } --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------
} There was a recent string on the listserv concerning substitutes for the } late, lamented Spurr's resin. Really? Is Spurr's gone? We just } ordered some from our usual suppliers and I haven't heard anything about } its being discontinued. Have I missed something (not impossible or } even unlikely some days)? } } Cheers, } Randy
Hi Randy, I'd be immensely grateful if you can provide me with a source. It's not available (it being the VCD - vinylcyclohexene dioxide - component of the mixture) here in the UK for sure, and the low viscosity resin substitutes I've tried are nowhere near as good for ease of sectioning and staining compared with Spurr resin. I'm almost tempted to ask you to let me know the source individually and not via the server, because I reckon the stocks will disappear rapidly....but I won't be that mean! Regards, Jules
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG
==============================Original Headers============================== 4, 24 -- From bafg3-at-sussex.ac.uk Wed Jan 28 10:48:56 2009 4, 24 -- Received: from karpinski.uscs.susx.ac.uk (karpinski.uscs.susx.ac.uk [139.184.14.85]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SGmtnW017822 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 10:48:55 -0600 4, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:4865) 4, 24 -- by karpinski.uscs.susx.ac.uk with esmtp (Exim 4.64) 4, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} ) 4, 24 -- id KE6XIJ-000JRA-B2 4, 24 -- for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 16:51:55 +0000 4, 24 -- Date: Wed, 28 Jan 2009 16:48:53 -0000 4, 24 -- To: Microscopy-at-microscopy.com 4, 24 -- Subject: Re: [Microscopy] Demise of Spurr's? 4, 24 -- Message-ID: {505719039.1233161332-at-ls0130.lifesci.susx.ac.uk} 4, 24 -- In-Reply-To: {200901281553.n0SFr91A031204-at-ns.microscopy.com} 4, 24 -- Originator-Info: login-token=Mulberry:01jMl4vu4q3iXACdgY9S26PO0xAC7j5e0kuj7j; 4, 24 -- token_authority=postmaster-at-central.susx.ac.uk 4, 24 -- X-Mailer: Mulberry/2.0.8 (Win32) 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- Content-Disposition: inline 4, 24 -- X-Sussex: true 4, 24 -- X-Sussex-transport: remote_smtp_rew 4, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk} ==============================End of - Headers==============================
Hi Randy, Yes, I'd love the attachments if they give the corrected formula for the new resin mix. I did try it before and it wasn't good for me! Appreciate your (and Dale's) help, Jules
--On 28 January 2009 10:52 -0600 "Tindall, Randy D." {TindallR-at-missouri.edu} wrote:
} Hi Jules, } } Hopefully the Dale Callahan post clears this up. I think we get the } same formulation as you, but they still call it "Spurr's". I can send } you Dale's attachments, if you like. } } Good luck, } Randy } } -----Original Message----- } From: j.r.thorpe-at-sussex.ac.uk [mailto:j.r.thorpe-at-sussex.ac.uk] } Sent: Wednesday, January 28, 2009 10:50 AM } To: Tindall, Randy D. } Subject: [Microscopy] Re: Demise of Spurr's? } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } --On 28 January 2009 09:53 -0600 TindallR-at-missouri.edu wrote: } } } ------------------------------------------------------------------------ } - } } --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } - } } } There was a recent string on the listserv concerning substitutes for } the } } late, lamented Spurr's resin. Really? Is Spurr's gone? We just } } ordered some from our usual suppliers and I haven't heard anything } about } } its being discontinued. Have I missed something (not impossible or } } even unlikely some days)? } } } } Cheers, } } Randy } } Hi Randy, } I'd be immensely grateful if you can provide me with a source. } It's } not available (it being the VCD - vinylcyclohexene dioxide - component } of } the mixture) here in the UK for sure, and the low viscosity resin } substitutes I've tried are nowhere near as good for ease of sectioning } and } staining compared with Spurr resin. } I'm almost tempted to ask you to let me know the source } individually and not via the server, because I reckon the stocks will } disappear rapidly....but I won't be that mean! } Regards, } Jules } } Dr. Julian R. Thorpe } (Office 2C9/Lab 2C11-13) } Electron Microscope Division, } The Sussex Centre for Advanced Microscopy, } John Maynard-Smith Building, } School of Life Sciences, } University of Sussex, } Falmer, } Brighton BN1 9QG } } ==============================Original } Headers============================== } 4, 24 -- From bafg3-at-sussex.ac.uk Wed Jan 28 10:48:56 2009 } 4, 24 -- Received: from karpinski.uscs.susx.ac.uk } (karpinski.uscs.susx.ac.uk [139.184.14.85]) } 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n0SGmtnW017822 } 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 } 10:48:55 -0600 } 4, 24 -- Received: from ls0130.lifesci.susx.ac.uk } ([139.184.162.69]:4865) } 4, 24 -- by karpinski.uscs.susx.ac.uk with esmtp (Exim 4.64) } 4, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} ) } 4, 24 -- id KE6XIJ-000JRA-B2 } 4, 24 -- for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 16:51:55 } +0000 } 4, 24 -- Date: Wed, 28 Jan 2009 16:48:53 -0000 } 4, 24 -- To: Microscopy-at-microscopy.com } 4, 24 -- Subject: Re: [Microscopy] Demise of Spurr's? } 4, 24 -- Message-ID: {505719039.1233161332-at-ls0130.lifesci.susx.ac.uk} } 4, 24 -- In-Reply-To: {200901281553.n0SFr91A031204-at-ns.microscopy.com} } 4, 24 -- Originator-Info: } login-token=Mulberry:01jMl4vu4q3iXACdgY9S26PO0xAC7j5e0kuj7j; } 4, 24 -- token_authority=postmaster-at-central.susx.ac.uk } 4, 24 -- X-Mailer: Mulberry/2.0.8 (Win32) } 4, 24 -- MIME-Version: 1.0 } 4, 24 -- Content-Type: text/plain; charset=us-ascii; format=flowed } 4, 24 -- Content-Transfer-Encoding: 7bit } 4, 24 -- Content-Disposition: inline } 4, 24 -- X-Sussex: true } 4, 24 -- X-Sussex-transport: remote_smtp_rew } 4, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk} } ==============================End of - } Headers==============================
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
At 12:04 PM 1/28/2009, j.r.thorpe-at-sussex.ac.uk wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 16, 27 -- From jd-at-laddresearch.com Wed Jan 28 12:45:41 2009 16, 27 -- Received: from bean.electric.net (bean.electric.net [72.35.23.29]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SIjfdG028389 16, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:45:41 -0600 16, 27 -- Received: from 1LSFPu-0001wV-WC by bean.electric.net with emc1-ok (Exim 4.69) 16, 27 -- (envelope-from {jd-at-laddresearch.com} ) 16, 27 -- id 1LSFPv-0001y1-Uh; Wed, 28 Jan 2009 10:45:39 -0800 16, 27 -- Received: by emcmailer; Wed, 28 Jan 2009 10:45:39 -0800 16, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 16, 27 -- by bean.electric.net with esmtps (TLSv1:AES256-SHA:256) 16, 27 -- (Exim 4.69) 16, 27 -- (envelope-from {jd-at-laddresearch.com} ) 16, 27 -- id 1LSFPu-0001wV-WC; Wed, 28 Jan 2009 10:45:39 -0800 16, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 27 -- Date: Wed, 28 Jan 2009 13:45:29 -0500 16, 27 -- To: j.r.thorpe-at-sussex.ac.uk 16, 27 -- From: jd {jd-at-laddresearch.com} 16, 27 -- Subject: Re: [Microscopy] Demise of Spurr's? 16, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 16, 27 -- In-Reply-To: {200901281704.n0SH4QP2019055-at-ns.microscopy.com} 16, 27 -- References: {200901281704.n0SH4QP2019055-at-ns.microscopy.com} 16, 27 -- Mime-Version: 1.0 16, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 16, 27 -- X-Outbound-IP: 216.204.198.170 16, 27 -- X-Env-From: jd-at-laddresearch.com 16, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 16, 27 -- Message-Id: {E1LSFPv-0001y1-Uh-at-bean.electric.net} ==============================End of - Headers==============================
I have a question regarding "pre-embedding staining" of 812 or Spurr's resin.
TEM is a useful tool for study of interaction of dental adhesives with dentin (which is mostly mineral). Unfortunately for me embedding resin and dental adhesive resin have about the same electron density, making it difficult, if not impossible, to tell them apart. It makes difficult to observe depth of infiltration, porosity, etc. So far for studies like these I have cut sections from not embedded teeth with mixed results.
I would greatly appreciate any suggestions about changing electron density of embedding media.
Many thanks in advance,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Years ago (1980s), I followed someone else's lead to dope epoxy resin with iodoform to increase its average atomic number.
I was studying minerals in coal and standard epoxy resins provided practically no contrast with coal in backscattered electron images. We ended up dissolving about 15 wt.% iodoform in epoxy resin. We later added the hardener and the epoxy behaved in much the same way as the original two-part epoxy. That is, hardness and polymerization were similar.
The resulting epoxy offered significant contrast with coal and allowed us to proceed with automated image analyses.
Be advised that iodoform is rather nasty and needs to be handled with care.
For what it's worth, I began working with iodoform shortly after the Right-to-know laws were passed. Suppliers had recently started including MSDSs with all their chemicals. I was still bemused that my chemical-grade calcium carbonate "should be disposed of in an approved, chemical-waste landfill" when my iodoform arrived. I had to do some more of my own research to determine if iodoform was really as nasty as the MSDS said or not. (It is.) Someone was crying wolf about the calcium carbonate while a contractor was laying tons of the stuff just outside our building as a base for the parking lot.
Warren S.
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Wednesday, January 28, 2009 1:03 PM To: wesaia-at-iastate.edu
Dear friends:
This is a message asking for help adjusting our LKB Knifemaker(s).
I am teaching an ultramicrotomy class to 20 community college students and we use glass knives. My job is to make sure the instruments are adjusted so they can make good knives.
We have 6, yes that's six, LKB Knifemakers in various states of (dis)repair. I need to build at least one good one from all of these.
Most of them were donated, some are better than others, I can probably figure out what to do, but just wanted to check with any experts on the list.
In another life, I used an LKB Knifemaker that worked pretty well. That one was serviced by an LKB tech once and a while and, as usual, had all the adjustments taped down with notes saying things like 'Do not adjust'. Since I could at least follow directions, I never tried to change the adjustments.
Now in my new life, I have 6 things that look like Knifemakers sitting on the bench and 20 sets of eyes looking at me expecting these things to work perfectly every time. I have done the expected Googling and found a few remarks about adjusting Knifemakers, but before I start tinkering, I wanted to check with the list to see if someone has the magic formula to insure success. Experience trumps google every time.
Thanks
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 42 -- From jkrupp-at-deltacollege.edu Wed Jan 28 14:30:26 2009 13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0SKUP5N000937 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 14:30:26 -0600 13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 42 -- by localhost (Email Security Appliance) with SMTP id EDFCB213D72_980BB12B 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT) 13, 42 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 13, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id E496A1870F2_980BB12F 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT) 13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 13, 42 -- with ESMTP id 45280969 for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 12:30:23 -0800 13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 13, 42 -- ESMTP id KE76WG00.4MS for {Microscopy-at-microscopy.com} ; Wed, 28 13, 42 -- Jan 2009 12:14:40 -0800 13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 9F5C78F74D04 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST) 13, 42 -- X-Virus-Scanned: amavisd-new at 13, 42 -- X-Spam-Flag: NO 13, 42 -- X-Spam-Score: -2.499 13, 42 -- X-Spam-Level: 13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 13, 42 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1] 13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 13, 42 -- with ESMTP id R34HTgsbNAlK for {Microscopy-at-microscopy.com} ; 13, 42 -- Wed, 28 Jan 2009 12:30:23 -0800 (PST) 13, 42 -- Received: from [172.20.2.74] (unknown [172.20.2.74]) 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 34B828F74CE5 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST) 13, 42 -- Message-Id: {615B1267-3305-4D49-90D8-39AB0E5E334C-at-deltacollege.edu} 13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 42 -- To: Microscopy-at-microscopy.com 13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 42 -- Content-Transfer-Encoding: 7bit 13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 13, 42 -- Subject: LKB Knifemaker 7800 13, 42 -- Date: Wed, 28 Jan 2009 12:30:22 -0800 13, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Another idea, especially since you have 6 of these miserable things. Rebuild one or two to make knives by the balanced-break method. How to do this is detailed by Herbert Hagler, Chap. 5, "Ultramicrotomy for Biological Electron Microscopy" pp67-96 in Electron MIcroscopy: Methods and Protocols, John Kuo, ed., 2nd edition. Specifically, Note 1 pg 91 ff. I only have one knife-maker and it works well enough to torment students, so I haven't done this. If I had 2, I would've by now.
Phil
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==============================Original Headers============================== 5, 26 -- From oshel1pe-at-cmich.edu Wed Jan 28 15:38:42 2009 5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SLcgmJ023273 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 15:38:42 -0600 5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n0SLccFS005101; 5, 26 -- Wed, 28 Jan 2009 16:38:38 -0500 5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 5, 26 -- Wed, 28 Jan 2009 16:38:15 -0500 5, 26 -- Mime-Version: 1.0 5, 26 -- Message-Id: {f06240812c5a67f74468f-at-[141.209.160.249]} 5, 26 -- In-Reply-To: {200901282033.n0SKXsOq010961-at-ns.microscopy.com} 5, 26 -- References: {200901282033.n0SKXsOq010961-at-ns.microscopy.com} 5, 26 -- Date: Wed, 28 Jan 2009 16:38:12 -0500 5, 26 -- To: jkrupp-at-deltacollege.edu 5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 26 -- Subject: Re: [Microscopy] LKB Knifemaker 7800 5, 26 -- Cc: Microscopy-at-microscopy.com 5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 26 -- X-OriginalArrivalTime: 28 Jan 2009 21:38:15.0225 (UTC) FILETIME=[B9FA9290:01C98190] 5, 26 -- X-Canit-CHI2: 0.00 5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 5, 26 -- X-CanItPRO-Stream: default 5, 26 -- X-Canit-Stats-ID: 8097891 - 412a4c8ab3d3 5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The magic formula is the manual. You didn't mention having one. It goes over all the features of the knives and adjustments to get good ones. It is really very simple once you get into it - only about 4 or 5 things to tweek.
These things are fairly rugged. There is a trick to getting the movable overhead clamping part off; You position the clamping lever at ~45o to the front (or back??) and lift and it slides up and off. You may need to clean the mating faces well with solvent to remove the sticky grease you may find there. These parts are brass and only need to be clean. The scoring part that you pull out is the same (clean) but I do use some silicone lube lightly on it; never anything that would get gummy, and some very light grease on the cam that the scoring wheel follows (inside the silver outer tube; again here start with it clean, and use sparingly a light grease only on the cam face, not the scoring wheel.
Note that the score wheel can be set for cutting squares from strips (the parallel lines) or scoring squares for knives - the square with a line symbol; these settings determine where the scoring wheel drops onto the glass and where it lifts off, very important.
The twiddle knobs at the top and bottom of the glass-position clamping forks position the glass square so that the { {fixed} } score falls on it just so. At the top and bottom there are 2 adjustments one is side-side and one clamps the metal fork that contacts the knife corner to set the position of the glass for and aft; the front one sets the position and the rear fork only applies pressure. You can change the angle of the score relative to the corners, and control where the score lifts off the square at the near corner by adjusting the clamping forks side-to-side and fore and aft; the side-side can be done at both top and bottom to accentuate your frustration but eventually place the score in the correct position. Adjustment for a score to a position very close to the near corner is critical for a controlled break; the break should come out on the right-hand side of the near corner and within 0.5mm of the corner - and this will be the cutting edge of the good knife. In good adjustment you will get 2 knives that are fairly symmetrical but not completely. The other opposite cutting edge will be less controlled and that knife is rarely as good, but usually just fine for facing up blocks or semi-thins.
Scoring wheel must be sharp. Easily replaced. I have a source for them if you need new ones. They are a standard size still available. Google works too.
Also the scoring pressure must be light - thus the need for the sharp wheel; too much pressure, too deep and the score is broad and knives will not break smoothly and be erratic in shape. A gentle breaking pressure and slow break is preferred for the best knives.
And all glass is not created equal. If you don't have a stock see if someone can make a recommendation. I have a lot of old LKB glass and it is still very good. People have brought newer glass and some of it does not break so well.
Let me know if you need a copy of the instructions w/pictures - the originals with drawings of the score marks, angles, etc. are very helpful.
Dale
jkrupp-at-deltacollege.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear friends: } } This is a message asking for help adjusting our LKB Knifemaker(s). } } I am teaching an ultramicrotomy class to 20 community college students } and we use glass knives. My job is to make sure the instruments are } adjusted so they can make good knives. } } We have 6, yes that's six, LKB Knifemakers in various states of } (dis)repair. I need to build at least one good one from all of these. } } Most of them were donated, some are better than others, I can probably } figure out what to do, but just wanted to check with any experts on } the list. } } In another life, I used an LKB Knifemaker that worked pretty well. } That one was serviced by an LKB tech once and a while and, as usual, } had all the adjustments taped down with notes saying things like 'Do } not adjust'. Since I could at least follow directions, I never tried } to change the adjustments. } } Now in my new life, I have 6 things that look like Knifemakers sitting } on the bench and 20 sets of eyes looking at me expecting these things } to work perfectly every time. I have done the expected Googling and } found a few remarks about adjusting Knifemakers, but before I start } tinkering, I wanted to check with the list to see if someone has the } magic formula to insure success. Experience trumps google every time. } } Thanks } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original Headers============================== } 13, 42 -- From jkrupp-at-deltacollege.edu Wed Jan 28 14:30:26 2009 } 13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0SKUP5N000937 } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 14:30:26 -0600 } 13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 42 -- by localhost (Email Security Appliance) with SMTP id EDFCB213D72_980BB12B } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT) } 13, 42 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) } 13, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id E496A1870F2_980BB12F } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT) } 13, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) } 13, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) } 13, 42 -- with ESMTP id 45280969 for Microscopy-at-microscopy.com; Wed, 28 Jan 2009 12:30:23 -0800 } 13, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by } 13, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 42 -- ESMTP id KE76WG00.4MS for {Microscopy-at-microscopy.com} ; Wed, 28 } 13, 42 -- Jan 2009 12:14:40 -0800 } 13, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 9F5C78F74D04 } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST) } 13, 42 -- X-Virus-Scanned: amavisd-new at } 13, 42 -- X-Spam-Flag: NO } 13, 42 -- X-Spam-Score: -2.499 } 13, 42 -- X-Spam-Level: } 13, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 } 13, 42 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1] } 13, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 42 -- with ESMTP id R34HTgsbNAlK for {Microscopy-at-microscopy.com} ; } 13, 42 -- Wed, 28 Jan 2009 12:30:23 -0800 (PST) } 13, 42 -- Received: from [172.20.2.74] (unknown [172.20.2.74]) } 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 34B828F74CE5 } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST) } 13, 42 -- Message-Id: {615B1267-3305-4D49-90D8-39AB0E5E334C-at-deltacollege.edu} } 13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 42 -- To: Microscopy-at-microscopy.com } 13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 13, 42 -- Content-Transfer-Encoding: 7bit } 13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 13, 42 -- Subject: LKB Knifemaker 7800 } 13, 42 -- Date: Wed, 28 Jan 2009 12:30:22 -0800 } 13, 42 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 20 -- From dac-at-research.umass.edu Wed Jan 28 15:44:31 2009 12, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SLiUnn000740 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 15:44:31 -0600 12, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged)) 12, 20 -- (authenticated bits=0) 12, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0SLiTJx031617 12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 16:44:30 -0500 12, 20 -- Message-ID: {4980D1FA.6080608-at-research.umass.edu} 12, 20 -- Date: Wed, 28 Jan 2009 16:45:30 -0500 12, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 12, 20 -- MIME-Version: 1.0 12, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 12, 20 -- Subject: Re: [Microscopy] LKB Knifemaker 7800 12, 20 -- References: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com} 12, 20 -- In-Reply-To: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com} 12, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both guosheng.liu-at-usask.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: guosheng.liu-at-usask.ca Name: Guosheng Liu
Organization: Biology Dept, Univ of Saskatchewan
Title-Subject: [Filtered] looking for "free" parts for Philips 505 SEM
Question: Dear All:
Our old Philips 505 SEM stopped working for a while and I still want to re-energize it at a low cost if possible.
Due to no immediate budget available for ordering new ones, I am wondering if there is a similar model SEM sitting around but we can salvage some parts (see following) from it before it is disposed. Detailed salvaging /shipping fees could be discussed later.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Cooperative Oxford Laboratory
Title-Subject: [Filtered] Paragon stain TEM
Question: I have checked the listserver for Paragon staining of epoxy resin and found several very helpful comments. I tried using Martin's procedure without success. I came across another comment about etching the slides first... Does anyone have experience with this?
I have since tried Richardson's stain and it just doesn't give enough differentiation. I am staining coral tissues that have been decalcified in 10% EDTA and fixed in 2% Gluteraldehyde, postfixed in 1% Osmium and embedded in Spurr's.
I can't find my (antique) notes on etching at the moment, but I can sort of remember, and it might get you started while someone else comes up with something. I was etching epoxy resins with saturated ethanolic NaOH to do PAS.
Make a solution of saturated ethanolic NaOH by dumping a lot of NaOH pellets in a bottle of absolute ethanol, like an inch and a half in a pint bottle. Put it aside for a couple of weeks until it looks like cognac. Soak slides in this solution in a coplin jar for (and this is where I can't remember - Two hours? Two days?). Sections used to easily come off those old, plain slides, so I was careful not to agitate. I think they would stay on better with Superfrost Plus or treated slides. Go ahead and start making your cognac .. er.. etching solution and I'll look for my PAS protocol.
Aloha from chilly Hawaii (OK, so it's 67F) Tina
} Organization: Cooperative Oxford Laboratory } } Title-Subject: [Filtered] Paragon stain TEM } } Question: I have checked the listserver for Paragon staining of epoxy } resin and found several very helpful comments. I tried using } Martin's procedure without success. I came across another comment } about etching the slides first... Does anyone have experience with } this? } } I have since tried Richardson's stain and it just doesn't give enough } differentiation. I am staining coral tissues that have been } decalcified in 10% EDTA and fixed in 2% Gluteraldehyde, postfixed in } 1% Osmium and embedded in Spurr's. } } Hope you can help. } Sue } } Login Host: 205.156.36.37 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 18:23:26 2009 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T0NPRl022677 } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 18:23:26 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c5a6a764137a-at-[206.69.208.22]} } 8, 11 -- Date: Wed, 28 Jan 2009 18:23:25 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Paragon stain TEM } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 28 22:01:19 2009 8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T41IS7022534 8, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 22:01:18 -0600 8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n0T41Eox020137 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 21 -- Wed, 28 Jan 2009 18:01:14 -1000 (HST) 8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n0T41BjA020134; 8, 21 -- Wed, 28 Jan 2009 18:01:13 -1000 (HST) 8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 8, 21 -- Date: Wed, 28 Jan 2009 18:01:10 -1000 (HST) 8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 21 -- X-Sender: tina-at-halia 8, 21 -- To: sue.tyler-at-noaa.gov 8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 21 -- Subject: Re: [Microscopy] viaWWW: Paragon stain TEM 8, 21 -- In-Reply-To: {200901290024.n0T0OCU6025080-at-ns.microscopy.com} 8, 21 -- Message-ID: {Pine.GSO.4.21.0901281752000.20054-100000-at-halia} 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I prefer KOH in MeOH. We use 5' etch, followed by 2 changes of absolute MeOH. We published a protocol for Iron Hematoxylin/Eosin/Alcian blue (which gives pretty differentiated staining on marine inverts) a while back in Microskopie; I can send a .doc file to anyone interested. If you use PAS after etching, watch for staining artifact--epoxy embedding generates a bunch of 'em, at least with our invert material. Julian
tina-at-pbrc.hawaii.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, Sue- } } I can't find my (antique) notes on etching at the moment, but I can sort } of remember, and it might get you started while someone else comes up with } something. I was etching epoxy resins with saturated ethanolic NaOH to do } PAS. } } Make a solution of saturated ethanolic NaOH by dumping a lot of NaOH } pellets in a bottle of absolute ethanol, like an inch and a half in a pint } bottle. Put it aside for a couple of weeks until it looks like cognac. } Soak slides in this solution in a coplin jar for (and this is where I } can't remember - Two hours? Two days?). Sections used to easily come off } those old, plain slides, so I was careful not to agitate. I think they } would stay on better with Superfrost Plus or treated slides. Go ahead and } start making your cognac .. er.. etching solution and I'll look for my } PAS protocol. } } Aloha from chilly Hawaii (OK, so it's 67F) } Tina } } } } } Organization: Cooperative Oxford Laboratory } } } } Title-Subject: [Filtered] Paragon stain TEM } } } } Question: I have checked the listserver for Paragon staining of epoxy } } resin and found several very helpful comments. I tried using } } Martin's procedure without success. I came across another comment } } about etching the slides first... Does anyone have experience with } } this? } } } } I have since tried Richardson's stain and it just doesn't give enough } } differentiation. I am staining coral tissues that have been } } decalcified in 10% EDTA and fixed in 2% Gluteraldehyde, postfixed in } } 1% Osmium and embedded in Spurr's. } } } } Hope you can help. } } Sue } } } } Login Host: 205.156.36.37 } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 8, 11 -- From zaluzec-at-microscopy.com Wed Jan 28 18:23:26 2009 } } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T0NPRl022677 } } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 18:23:26 -0600 } } 8, 11 -- Mime-Version: 1.0 } } 8, 11 -- Message-Id: {p06240801c5a6a764137a-at-[206.69.208.22]} } } 8, 11 -- Date: Wed, 28 Jan 2009 18:23:25 -0600 } } 8, 11 -- To: microscopy-at-microscopy.com } } 8, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver) } } 8, 11 -- Subject: viaWWW: Paragon stain TEM } } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } ==============================End of - Headers============================== } } } } } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } } ==============================Original Headers============================== } 8, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 28 22:01:19 2009 } 8, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0T41IS7022534 } 8, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 22:01:18 -0600 } 8, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } 8, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n0T41Eox020137 } 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); } 8, 21 -- Wed, 28 Jan 2009 18:01:14 -1000 (HST) } 8, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n0T41BjA020134; } 8, 21 -- Wed, 28 Jan 2009 18:01:13 -1000 (HST) } 8, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs } 8, 21 -- Date: Wed, 28 Jan 2009 18:01:10 -1000 (HST) } 8, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 8, 21 -- X-Sender: tina-at-halia } 8, 21 -- To: sue.tyler-at-noaa.gov } 8, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} } 8, 21 -- Subject: Re: [Microscopy] viaWWW: Paragon stain TEM } 8, 21 -- In-Reply-To: {200901290024.n0T0OCU6025080-at-ns.microscopy.com} } 8, 21 -- Message-ID: {Pine.GSO.4.21.0901281752000.20054-100000-at-halia} } 8, 21 -- MIME-Version: 1.0 } 8, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } ==============================End of - Headers============================== } }
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
Several people have now asked about the scoring wheel source. I have not bought from this company, but I had found this when I searched a while back. http://www.glasscuttingwheels.com/cw.html (what else would you expect?)
I have measured the LKB wheels and they are 5.0mm dia, with a 1.4mm hole and 1.1mm thick, so the wheels are correct. The edge angle this company recommends for ~6mm thick glass is 144degrees and this is what the LKB wheels look like; I can photograph and measure the angle with ImageJ if anyone requests it. The axles may be more of an issue - the original ones are 6.5mm long and that size is not avaialble. The mechanism can only accommodate a 6.5mm length (it is precisely 7.0mm wide); they offer a 4mm and a 9.25mmlength, but a machinist could easily grind the longer one to length. The 4mm would just hold the wheel but not well. The old axles in my box show significant wear so they probably should be replaced as a pair, the way they were sold by LKB.
I still have a number of original, new, wheels and axles, if we need them to make measurements.
Hope this helps.
Dale
Taylor, Jeannette wrote: } Dear Dale, would you please send me your source for new scoring wheels? We have an LKB Knifemaker 7800 and it works fine but the scoring wheel needs to be replaced as it is getting dull. } } Thanks, Jeannette } } Jeannette Taylor } Technologist II } Robert P. Apkarian Integrated Electron Microscopy Core } Emory University } 1515 Dickey Drive } Atlanta, Georgia 30322 } } jvtaylo-at-emory.edu } 404-712-8674 } } } This e-mail message (including any attachments) is for the sole use of } the intended recipient(s) and may contain confidential and privileged } information. If the reader of this message is not the intended } recipient, you are hereby notified that any dissemination, distribution } or copying of this message (including any attachments) is strictly } prohibited. } } If you have received this message in error, please contact } the sender by reply e-mail message and destroy all copies of the } original message (including attachments).
==============================Original Headers============================== 6, 22 -- From dac-at-research.umass.edu Thu Jan 29 09:54:27 2009 6, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0TFsPFn008406 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 09:54:27 -0600 6, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 22 -- (authenticated bits=0) 6, 22 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0TFsNJB005632 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 10:54:23 -0500 6, 22 -- Message-ID: {4981D181.7040201-at-research.umass.edu} 6, 22 -- Date: Thu, 29 Jan 2009 10:55:45 -0500 6, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 22 -- Reply-To: dac-at-research.umass.edu 6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 6, 22 -- MIME-Version: 1.0 6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] Re: LKB Knifemaker 7800 scoring wheels 6, 22 -- References: {200901282144.n0SLiteV001833-at-ns.microscopy.com} {99BAF15EB8A4F348949A061CACFD22B90119F3E75AFF-at-EXCHANGE20.Enterprise.emory.net} 6, 22 -- In-Reply-To: {99BAF15EB8A4F348949A061CACFD22B90119F3E75AFF-at-EXCHANGE20.Enterprise.emory.net} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Ah, I found my old protocol, although I'll bet Julian's is more current, and may give you the differentiation you're looking for.
Add 100-150g anhydrous NaOH pellets to 250 ml freshly opened bottle of absolute ethanol. Allow to stand until cognac or deep rust-brown color, shaking occasionally, for about a week. Keeps 4-5 weeks. Store in plastic bottle, if possible. To remove resin from sections, immerse slides in solution for an hour or more, checking to see when resin is etched away (I remember this being about two hours for 0.5 micrometer thick sections). Drain well, but don't blot. Immerse slides in 4 changes of absolute ethanol, the proceed with staining, clearing, and mounting. This was originally for PAS (Periodic Acid Schiff) on gecko reproductive tissue. Now I want to try Julian's recipe on coral reproductive tissue...
Aloha, Tina
} I prefer KOH in MeOH. We use 5' etch, followed by 2 changes of absolute } MeOH. We published a protocol for Iron Hematoxylin/Eosin/Alcian blue } (which gives pretty differentiated staining on marine inverts) a while } back in Microskopie; I can send a .doc file to anyone interested. } If you use PAS after etching, watch for staining artifact--epoxy } embedding generates a bunch of 'em, at least with our invert material. } Julian } } -- } Julian P.S. Smith III } Director, Winthrop Microscopy Facility } Dept. of Biology } Winthrop University } 520 Cherry Rd. } Rock Hill, SC 29733 } } 803-323-2111 x6427 (vox) } 803-323-3448 (fax) } 803-524-2347 (cell)
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 21 -- From tina-at-pbrc.hawaii.edu Thu Jan 29 16:56:05 2009 6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0TMu4NB029533 6, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 29 Jan 2009 16:56:05 -0600 6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n0TMu1EQ023345 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 21 -- Thu, 29 Jan 2009 12:56:02 -1000 (HST) 6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n0TMu0tE023342; 6, 21 -- Thu, 29 Jan 2009 12:56:01 -1000 (HST) 6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 21 -- Date: Thu, 29 Jan 2009 12:56:00 -1000 (HST) 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 21 -- X-Sender: tina-at-halia 6, 21 -- To: smithj-at-winthrop.edu 6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] viaWWW: etching epoxy sections 6, 21 -- In-Reply-To: {200901291354.n0TDs0P9024319-at-ns.microscopy.com} 6, 21 -- Message-ID: {Pine.GSO.4.21.0901291246440.23318-100000-at-halia} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
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Email: eric-at-unquantum.net Name: Eric Reiter
Organization: Unqunatum
Title-Subject: [Filtered] Genie software
Question: I have a Aptec series 5000 x-ray spectroscopy card: Looking for
Aptec spectroscopy software, or Canberra Genie software for older 486-like computers in DOS or up to windows 2000.
Would like to buy this pulse height analysis software.
As there is again some demand here for observations of TEM grids by SEM, I took out of the drawer the home made TEM grids holders I had machined a while ago. And non of them is really practical to use. The first was made with an EM300 tip, and works right, but it's a one shot and for limited to the jeol 840 serie stage. The second is delicate to use, and I fear to bent the grids each time I take them away from the holder.
So what kind of holder do other use for TEM grids (only SEM observations, no STEM), which allows very short WD and an easy way to mount/umount 5-6 grids in a batch ? I've soon looked in some catalogues, but certainly not at all sources. And users advices are very usfull !
I'm interested in any ideas and/or squetches for home manufacturing in our workshop, and/or for documentions, users advices and manufacturer doc and quoting etc.
Thanks in advance, and have a good WE !
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
As there is again some demand here for observations of TEM grids by SEM, I took out of the drawer the home made TEM grids holders I had machined a while ago. And non of them is really practical to use. The first was made with an EM300 tip, and works right, but it's a one shot and for limited to the jeol 840 serie stage. The second is delicate to use, and I fear to bent the grids each time I take them away from the holder.
So what kind of holder do other use for TEM grids (only SEM observations, no STEM), which allows very short WD and an easy way to mount/umount 5-6 grids in a batch ? I've soon looked in some catalogues, but certainly not at all sources. And users advices are very usfull !
I'm interested in any ideas and/or squetches for home manufacturing in our workshop, and/or for documentions, users advices and manufacturer doc and quoting etc.
Thanks in advance, and have a good WE !
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hello listers, I was asked recently about accuracy of measuring electron diffraction patterns, and seemed to remember something in the MSA listserver from about 10 years ago, forgetting that I was the one who asked the question in the first place!
I'm not going for the record of longest running thread on the list, but I do think it's worth asking the question again, particularly now there are much better digital cameras (and aberration corrected machines), as well as about 2^6 times more processing power in the standard computer - which means 32-bit image processing is feasible, for example.
I don't expect any corrections to previous postings (Jan 1999) on the basic physics (I hope) but it would be interesting to see how things have moved on. Also I received 21 answers in 3 days back then, it will be interesting to see if it's still as lively now.
And are there any other questions which were asked a decade ago which should be re-visited?
Thanks
Richard Beanland
*Date: Tue, 05 Jan 1999 10:31:53 +0000 (GMT)
On Jan 30, 2009, at 9:06 AM, contact-at-integrityscientific.com wrote:
} I would like to get some information on TEM diffraction pattern } analysis. Specifically; } } 1) What software is available for analysis of diffraction patterns } (both } ring patterns and spot patterns)? What kind of accuracy can be } obtained } - are we getting close to the accuracy of X-ray diffractometry yet, or } are there fundamental reasons such as lens aberrations, smaller Bragg } angles, and accuracy of measurement which mean that we'll never get } there? } } 2) What are the typical procedures people use for, say, measuring } camera } length or identifying unknown phases using diffraction? } Dear Richard, I certainly do not know all the existing software, but I do know that there is the SP operation in SPIDER that can identify lattice points, find the centers and radii of rings, and determine intensity values. When I was doing SAED to determine the structure of phthalocyanines, I wrote a script that performed a background subtraction in two steps. The first step put boxes around all the spots, replaced the pixels inside the boxes with values that were the average of the edges of the boxes, then took a radial average (the center of which was the center of the lattice). This was then subtracted from the ED pattern, which got rid of the non-linear background and didn't have to be exact, since the second step was a bilinear background subtraction. The resulting intensities were sufficiently accurate to give reliable structure determinations. I published several articles with Doug Dorset and Jim Turner about this in the early '90s. Neither lens aberrations, nor small Bragg angles present practical problems for structure determinations, but dynamical scattering is a serious issue, which was overcome in my work by operating at 1200 kV, which reduced dynamical effects to a manageable level. Although I have never done any CBED, I have heard reports at M&M detailing the information that can be obtained, and these said that the low-order spots gave the most accurate data on such features as the distribution of electrons in chemical bonds. these data were more accurate than could be obtained by any other method, including X-ray diffraction. John Spence is the expert on this, so you may want to contact him. I evaporated gold onto the phthalocyanines and took SAED patterns from which the lattice constants of the phthalocyanines were determined. (This also determines the camera length.) Having both the gold and the specimen on the same grid controls for changes in camera length that may occur with variations in specimen height or other scope parameters. The phases were found by direct methods--in the case of the phthalocyanines, the Sayre equation and triplet formulas were sufficient, but either the tangent formula or maximum entropy methods should work also. I authored a paper in Acta Cryst. showing that these methods will work for electron diffraction, since they are a consequence of the unitary nature of scattering processes. The references in that paper are to work done by several people to which I added a small amount. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Jan 30 12:04:56 2009 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0UI4uCN025190 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 12:04:56 -0600 4, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 4, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 600B432AD0C 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 10:04:55 -0800 (PST) 4, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 4, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 4, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 15B1232AD40 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 30 Jan 2009 10:04:53 -0800 (PST) 4, 22 -- Message-Id: {1AD75A53-9FBE-4391-B672-7666D51C636D-at-caltech.edu} 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- In-Reply-To: {200901301706.n0UH6XUF003345-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 4, 22 -- Subject: Re: [Microscopy] TEM; diffraction pattern analysis 4, 22 -- Date: Fri, 30 Jan 2009 10:04:53 -0800 4, 22 -- References: {200901301706.n0UH6XUF003345-at-ns.microscopy.com} 4, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
You probably already saw these in your search, but how about:
1. Grid holders, used in rotary shadowing might be useful. Edwards, for example, makes a nice holder that keeps 11 grids in place on a circular holder. The holder could be inserted into the SEM and much like a conventional stub. You can see this device (E0857 1000 = 3 mm grid holder used with Rotatilt 3) on page 2 and 3 of the following brochure:
www.edwards.co.il/catalog/14/140200.pdf
2. The Grid Stick holder. It's a metal strip designed to hold grids in place for TEM staining. It does use a glue to tack the grids in place, however. Otherwise, it looks reasonable. You need to modify it to suit your needs. You can pursue this at:
3. Something like the Leica cryo-grid holder might work. This is a clamp that holds onto the edge of the grid and is held in place by a tightened screw. You may be able to make one using a single-edge razor blade. If the blade is attached to a large stub by means of a screw (such that the blade is flat against the stub surface), the edge of the blade could be used to hold the grids onto the stub. You can see the cryo-grid holder here:
René Haas, Gilbert De Murcia . 1985. A simple device for accurate and large scale rotary shadowing of spread biological specimens. Journal of Electron Microscopy Technique. Volume 2, Issue 5 , Pages 519 - 520.
Good luck.
John Bozzola
} As there is again some demand here for observations of TEM grids by SEM, } I took out of the drawer the home made TEM grids holders I had machined } a while ago. And non of them is really practical to use. The first was } made with an EM300 tip, and works right, but it's a one shot and for } limited to the jeol 840 serie stage. The second is delicate to use, and } I fear to bent the grids each time I take them away from the holder.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: blotocka-at-gmail.com Name: Barbara £otocka
Question: Question: Is there any procedure that allows to remove (dissolve) calcium oxalate crystals from plant samples prior to embedding? I have to cut semi- and ultrathin sections of a plant organ that is virtually studded with druses. The hand sections look absolutely stunning in polarization, but both paraffin and hard-grade epoxy sections are scratched to shreds. I considered soaking the osmium-contrasted samples in some acidic buffer prior to dehydration - but perhaps someone solved this problem already?
Jacques, I had a holder made in our shop some years back out of a 10mm dia cylindrical carbon SEM stub which would accomodates 4 TEM grids. One could start with a 25mm dia carbon stub and accomodate many more. Fabrication involved milling 4 equallly spaced 3.1mm dia depressions 0.5-1.0mm deep in the top of the stubso that the edge of each depression was about 1mm from the edge of the stub.Then, a smaller diameter (~2.5mm dia) depression was bored about 10mm deep inthe center of each original depression to provide a small flange to support the TEM grid and to act as as a beam "sink." A small radial notch about 0.5 mm wide was cut from the outer perimeter of the stub into the depression down to a bit below the flange to allow tweezers to be used to place and remove each grid. In my JSM-35 the evacuation of the airlock was gentle enough that no retainers were needed to hold the grids in place. Later, we bored vents from the side of the stub into the bottom of each inner depression to allow an alternate path for the air to escape during pumpdown, and some small split rings (similar to the old Philips circlip which can't be used here because it is too stiff) were used to retain each grid.The rings were made by tightly wrapping many turns of #32 tinned copper wire around a 3/32" drill bit and then slitting the helix while still on the bit with a sharp razor blade along the bit's length. Some adjustment and flattening was necessary to make the clip fit snugly into the depression. These latter modifications were necessary because when the holder was used in microscopes with a more vigorous pump- down, the grids were sometimes blown out of the holder. If you don't need the "sink" under the grid depression, you can omit it and possibly avoid the blowout problem in a much simpler way. Perhaps this is more trouble than you want to go to, but if you're interested contact me off list, and I can take a picture of the holder to send to you.
On 30 Jan 2009 at 11:02, jacques.faerber-at-ipcms.u-strasbg.fr wrote:
} } Hi all } } As there is again some demand here for observations of TEM grids by } SEM, I took out of the drawer the home made TEM grids holders I had } machined a while ago. And non of them is really practical to use. The } first was made with an EM300 tip, and works right, but it's a one } shot and for limited to the jeol 840 serie stage. The second is } delicate to use, and I fear to bent the grids each time I take them } away from the holder. } } So what kind of holder do other use for TEM grids (only SEM } observations, no STEM), which allows very short WD and an easy way to } mount/umount 5-6 grids in a batch ? I've soon looked in some } catalogues, but certainly not at all sources. And users advices are } very usfull ! } } I'm interested in any ideas and/or squetches for home manufacturing in } our workshop, and/or for documentions, users advices and manufacturer } doc and quoting etc. } } Thanks in advance, and have a good WE ! } } -- } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr Sincerely yours, Andy Buechele Andrew C. Buechele, Ph.D. The Catholic University of America - VSL 409 Hannan Hall Washington, D.C. 20064 Phone: 202-319-4995 Fax: 202-319-4469
==============================Original Headers============================== 7, 30 -- From andrewb-at-vsl.cua.edu Fri Jan 30 20:23:11 2009 7, 30 -- Received: from mail.vsl.cua.edu (interface.vsl.cua.edu [136.242.188.2]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0V2N9gX021493 7, 30 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 20:23:10 -0600 7, 30 -- Received: from localhost (localhost [127.0.0.1]) 7, 30 -- by mail.vsl.cua.edu (Postfix) with ESMTP id 4C6459C1D09 7, 30 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 21:23:09 -0500 (EST) 7, 30 -- X-Virus-Scanned: amavisd-new at vsl.cua.edu 7, 30 -- Received: from mail.vsl.cua.edu ([127.0.0.1]) 7, 30 -- by localhost (pandora.vsl.cua.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 30 -- with ESMTP id sWSt5SgzHNzj for {Microscopy-at-Microscopy.Com} ; 7, 30 -- Fri, 30 Jan 2009 21:23:07 -0500 (EST) 7, 30 -- Received: from [136.242.189.103] (unknown [136.242.189.103]) 7, 30 -- by mail.vsl.cua.edu (Postfix) with ESMTP id 217349C1CFD 7, 30 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 Jan 2009 21:23:07 -0500 (EST) 7, 30 -- From: "Andrew Buechele" {andrewb-at-vsl.cua.edu} 7, 30 -- To: Microscopy-at-Microscopy.Com 7, 30 -- Date: Fri, 30 Jan 2009 21:23:06 -0500 7, 30 -- MIME-Version: 1.0 7, 30 -- Subject: Re: [Microscopy] SEM holder for TEM grids 7, 30 -- Reply-to: andrewb-at-vsl.cua.edu 7, 30 -- Message-ID: {49836FBA.10981.1F562EB-at-andrewb.vsl.cua.edu} 7, 30 -- Priority: normal 7, 30 -- In-reply-to: {200901301702.n0UH2vuU027567-at-ns.microscopy.com} 7, 30 -- References: {200901301702.n0UH2vuU027567-at-ns.microscopy.com} 7, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 7, 30 -- Content-type: text/plain; charset=ISO-8859-1 7, 30 -- Content-description: Mail message body 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id n0V2N9gX021493 ==============================End of - Headers==============================
EBSA 2009 is the 7th Congress of the European Biophysical Societies' Association (EBSA), formed in 1984, with the objectives to advance and disseminate knowledge of the principles, recent developments and applications of biophysics, and to foster the exchange of scientific information among biophysicists. EBSA2009 provides special incentives for young investigators. The Congress will also celebrate 25 years of EBSA.
Have a look to the Pleanry speakers list and Scientific program. Bursaries for students will be available and Accommodations at 17 Euros per night including breakfast, too.
Visit www.ebsa2009.org.
All the best Alby ---------------------------------------------------- "Water slowly flowed under the sky" (Cesare Pavese) ----------------------------------------------------- Alberto Diaspro, LAMBS IFOM IEO -MicroSCoBio, NBT-IIT, IBF-CNR Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it;
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==============================Original Headers============================== 5, 20 -- From sekkio-at-mac.com Sun Feb 1 03:01:58 2009 5, 20 -- Received: from asmtpout018.mac.com (asmtpout018.mac.com [17.148.16.93]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1191wfY011645 5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 1 Feb 2009 03:01:58 -0600 5, 20 -- MIME-version: 1.0 5, 20 -- Content-transfer-encoding: 7BIT 5, 20 -- Content-type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 20 -- Received: from [10.0.1.3] 5, 20 -- (host49-140-dynamic.50-82-r.retail.telecomitalia.it [82.50.140.49]) 5, 20 -- by asmtp018.mac.com 5, 20 -- (Sun Java(tm) System Messaging Server 6.3-7.03 (built Aug 7 2008; 32bit)) 5, 20 -- with ESMTPSA id {0KED00BR2QF6R510-at-asmtp018.mac.com} for 5, 20 -- microscopy-at-microscopy.com; Sun, 01 Feb 2009 01:01:58 -0800 (PST) 5, 20 -- Message-id: {6AC04C2B-5B53-43C8-B8C4-0473D2A36F73-at-mac.com} 5, 20 -- From: Alberto Diaspro {sekkio-at-mac.com} 5, 20 -- To: microscopy-at-microscopy.com, by MicroscopyListServer {venu-at-purdue.edu} 5, 20 -- Reply-to: Alberto Diaspro {diaspro-at-fisica.unige.it} , staff-at-ebsa2009.org 5, 20 -- Subject: July 11-15 2009 - Win in Science come to Genoa 5, 20 -- Date: Sun, 01 Feb 2009 10:01:53 +0100 5, 20 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
MSA's Project MICRO (Microscopy In Curriculum - Research Outreach) supports education about the microworld for middle school students. That means a curriculum manual for teachers, volunteers to help in classrooms, and advice and support in an extensive web page on MSA's site: http://www.microscopy.org/ProjectMICRO. That page includes a reviewed listing of over 150 children's books, other media, and websites about microscopy and the microworld. Nanotechnology isn't microscopy, but it certainly is part of the microworld. So MICRO has just added a new database of all the books for the same age group that MICRO has been able to find. Please take a look! The same information will appear in Microscopy Today later this year. -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
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From reginarus-at-shaw.ca.an Mon Feb 2 01:33:17 2009 Return-Path: {reginarus-at-shaw.ca.an} Received: from google.com (p54A26945.dip.t-dialin.net [84.162.105.69]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n127XFm7003465 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 2 Feb 2009 01:33:16 -0600 Received: from [175.131.115.172] (HELO google.com) by scentedmeat.de; Mon, 2 Feb 2009 08:24:49 +0100
Hi All,
Regarding sources for the scoring wheel for LKB knifebreakers, I received information that Leica Microsystems can also supply these parts...................
the usual disclaimer, I have no connection with Leica...... Dale
} You can buy them from Leica. } The order number is 16 70 52 27, 'scoring wheels for LKB knifemaker' } Yes the part number is for wheel and axle set, 3 parts each per pack. ............... } The rubber cushion we suggest not to use as it is not necessry. } I did not get my email onto the Listserver as it bounced back ! } } Pleased to be of assistance. } Best Wishes, } Ian } } Ian Lamswood } Marketing Manager } Hernalser Hauptstrasse 219 } 1170 Wien (Austria) } From: Dale Callaham {dac-at-research.umass.edu} } To: Ian Lamswood {ianlamswood44-at-yahoo.co.uk} } Sent: Friday, 30 January, 2009 17:42:51 } Subject: Re: [Microscopy] LKB Knifemaker 7800 scoring wheels } } Hi Ian, } } Thanks! That solves a lot of problems. Is this part number for a wheel+axle set? Are other components also avaialble? The rubber shock cushion is another part people will likely want to replace regularly since they get stiff/dead over time. Has Leica taken over the LKB Knifebreaker service also? } } Did your message go to the Microscopy List? You should echo it there so people know that Leica is a source for parts. } } Dale
Kim Davidson wrote: } Hi Dale, } } Would you share your source for the scoring wheel please? } } Thanks, } } kim } } ---------------------------------- } Kim Davidson } Department of Biomedical Sciences } Neurosciences Division } 1617 Campus Delivery } Colorado State University } Fort Collins, CO 80523-1617 } } 970-491-7389 (Phone) } 970-491-7907 (FAX) } } kim.davidson-at-colostate.edu } ---------------------------------- } } } } dac-at-research.umass.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Jon, } } } } The magic formula is the manual. You didn't mention having one. It goes } } over all the features of the knives and adjustments to get good ones. It } } is really very simple once you get into it - only about 4 or 5 things to } } tweek. } } } } These things are fairly rugged. There is a trick to getting the movable } } overhead clamping part off; You position the clamping lever at ~45o to } } the front (or back??) and lift and it slides up and off. You may need to } } clean the mating faces well with solvent to remove the sticky grease you } } may find there. These parts are brass and only need to be clean. The } } scoring part that you pull out is the same (clean) but I do use some } } silicone lube lightly on it; never anything that would get gummy, and } } some very light grease on the cam that the scoring wheel follows (inside } } the silver outer tube; again here start with it clean, and use sparingly } } a light grease only on the cam face, not the scoring wheel. } } } } Note that the score wheel can be set for cutting squares from strips } } (the parallel lines) or scoring squares for knives - the square with a } } line symbol; these settings determine where the scoring wheel drops onto } } the glass and where it lifts off, very important. } } } } The twiddle knobs at the top and bottom of the glass-position clamping } } forks position the glass square so that the { {fixed} } score falls on it } } just so. At the top and bottom there are 2 adjustments one is side-side } } and one clamps the metal fork that contacts the knife corner to set the } } position of the glass for and aft; the front one sets the position and } } the rear fork only applies pressure. You can change the angle of the } } score relative to the corners, and control where the score lifts off the } } square at the near corner by adjusting the clamping forks side-to-side } } and fore and aft; the side-side can be done at both top and bottom to } } accentuate your frustration but eventually place the score in the } } correct position. Adjustment for a score to a position very close to the } } near corner is critical for a controlled break; the break should come } } out on the right-hand side of the near corner and within 0.5mm of the } } corner - and this will be the cutting edge of the good knife. In good } } adjustment you will get 2 knives that are fairly symmetrical but not } } completely. The other opposite cutting edge will be less controlled and } } that knife is rarely as good, but usually just fine for facing up blocks } } or semi-thins. } } } } Scoring wheel must be sharp. Easily replaced. I have a source for them } } if you need new ones. They are a standard size still available. Google } } works too. } } } } Also the scoring pressure must be light - thus the need for the sharp } } wheel; too much pressure, too deep and the score is broad and knives } } will not break smoothly and be erratic in shape. A gentle breaking } } pressure and slow break is preferred for the best knives. } } } } And all glass is not created equal. If you don't have a stock see if } } someone can make a recommendation. I have a lot of old LKB glass and it } } is still very good. People have brought newer glass and some of it does } } not break so well. } } } } Let me know if you need a copy of the instructions w/pictures - the } } originals with drawings of the score marks, angles, etc. are very helpful. } } } } } } Dale } } } } jkrupp-at-deltacollege.edu wrote: } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Dear friends: } } } } } } This is a message asking for help adjusting our LKB Knifemaker(s). } } } } } } I am teaching an ultramicrotomy class to 20 community college students } } } and we use glass knives. My job is to make sure the instruments are } } } adjusted so they can make good knives. } } } } } } We have 6, yes that's six, LKB Knifemakers in various states of } } } (dis)repair. I need to build at least one good one from all of these. } } } } } } Most of them were donated, some are better than others, I can probably } } } figure out what to do, but just wanted to check with any experts on } } } the list. } } } } } } In another life, I used an LKB Knifemaker that worked pretty well. } } } That one was serviced by an LKB tech once and a while and, as usual, } } } had all the adjustments taped down with notes saying things like 'Do } } } not adjust'. Since I could at least follow directions, I never tried } } } to change the adjustments. } } } } } } Now in my new life, I have 6 things that look like Knifemakers sitting } } } on the bench and 20 sets of eyes looking at me expecting these things } } } to work perfectly every time. I have done the expected Googling and } } } found a few remarks about adjusting Knifemakers, but before I start } } } tinkering, I wanted to check with the list to see if someone has the } } } magic formula to insure success. Experience trumps google every time. } } } } } } Thanks } } } } } } Jon } } } } } } Jonathan Krupp } } } Delta College } } } 5151Pacific Ave. } } } Stockton, CA 95207 } } } 209-954-5284 } } } jkrupp-at-deltacollege.edu } } } } } } } } } } } } } } } ==============================Original Headers============================== } } } 13, 42 -- From jkrupp-at-deltacollege.edu Wed Jan 28 14:30:26 2009 } } } 13, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } } } 13, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n0SKUP5N000937 } } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 14:30:26 -0600 } } } 13, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } } } 13, 42 -- by localhost (Email Security Appliance) with SMTP id EDFCB213D72_980BB12B } } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 20:07:46 +0000 (GMT) } } } 13, 42 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) } } } 13, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id E496A1870F2_980BB12F } } } 13, 42 -- for {Microscopy-at-microscopy.com} ; 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} } } 13, 42 -- Wed, 28 Jan 2009 12:30:23 -0800 (PST) } } } 13, 42 -- Received: from [172.20.2.74] (unknown [172.20.2.74]) } } } 13, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 34B828F74CE5 } } } 13, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 12:30:23 -0800 (PST) } } } 13, 42 -- Message-Id: {615B1267-3305-4D49-90D8-39AB0E5E334C-at-deltacollege.edu} } } } 13, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } } } 13, 42 -- To: Microscopy-at-microscopy.com } } } 13, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } } } 13, 42 -- Content-Transfer-Encoding: 7bit } } } 13, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) } } } 13, 42 -- Subject: LKB Knifemaker 7800 } } } 13, 42 -- Date: Wed, 28 Jan 2009 12:30:22 -0800 } } } 13, 42 -- X-Mailer: Apple Mail (2.930.3) } } } ==============================End of - Headers============================== } } } } } } } ==============================Original Headers============================== } } 12, 20 -- From dac-at-research.umass.edu Wed Jan 28 15:44:31 2009 } } 12, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) } } 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n0SLiUnn000740 } } 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 15:44:31 -0600 } } 12, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged)) } } 12, 20 -- (authenticated bits=0) } } 12, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n0SLiTJx031617 } } 12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } } 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 28 Jan 2009 16:44:30 -0500 } } 12, 20 -- Message-ID: {4980D1FA.6080608-at-research.umass.edu} } } 12, 20 -- Date: Wed, 28 Jan 2009 16:45:30 -0500 } } 12, 20 -- From: Dale Callaham {dac-at-research.umass.edu} } } 12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 } } 12, 20 -- MIME-Version: 1.0 } } 12, 20 -- To: Microscopy List {microscopy-at-microscopy.com} } } 12, 20 -- Subject: Re: [Microscopy] LKB Knifemaker 7800 } } 12, 20 -- References: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com} } } 12, 20 -- In-Reply-To: {200901282035.n0SKZ1Bs014726-at-ns.microscopy.com} } } 12, 20 -- Content-Type: text/plain; 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==============================Original Headers============================== 9, 23 -- From dac-at-research.umass.edu Mon Feb 2 10:53:02 2009 9, 23 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12Gr0pe031194 9, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 10:53:01 -0600 9, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 9, 23 -- (authenticated bits=0) 9, 23 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n12GquFU010769 9, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 11:52:56 -0500 9, 23 -- Message-ID: {49872541.5070403-at-research.umass.edu} 9, 23 -- Date: Mon, 02 Feb 2009 11:54:25 -0500 9, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 23 -- Reply-To: dac-at-research.umass.edu 9, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 9, 23 -- MIME-Version: 1.0 9, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 23 -- Subject: Re: [Microscopy] Re: LKB Knifemaker 7800 - another source for scoring 9, 23 -- wheels. 9, 23 -- References: {200901282145.n0SLjs5Y005102-at-ns.microscopy.com} {4980D3F3.3040804-at-lamar.colostate.edu} 9, 23 -- In-Reply-To: {4980D3F3.3040804-at-lamar.colostate.edu} 9, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
From vicodin-at-hotmail.com Mon Feb 2 12:23:53 2009 Return-Path: {vicodin-at-hotmail.com} Received: from google.com (adsl190-28-129-18.epm.net.co [190.28.129.18]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12INqNX018858 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 2 Feb 2009 12:23:52 -0600 Received: from [151.196.113.82] (HELO google.com) by soggy-putdown.co.uk; Mon, 2 Feb 2009 13:23:53 -0500
Hi listers, We're having an issue with our EM grade glutaraldehyde. The last three batches of 2ml ampoules I've ordered have been very viscous and then form a sticky ppt when mixed with buffer. We have some 10ml ampoules that are fine. They have different lot numbers which makes me think it's batch dependent but before I return yet another box of glut, I was wondering if anyone else has had these issues. I've also noticed that the glut that isn't so thick becomes cloudy when first mixed and never has been before. If anyone has any ideas or has experienced the same things I'd like to hear from you. Thank you, Mary Gail Engle
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 Ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 FAX (859) 257-1581 University of KY Lexington, KY 40536
==============================Original Headers============================== 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 2009 5, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12KGgDV025817 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 14:16:42 -0600 5, 24 -- Received: from ex7hb02.ad.uky.edu ([128.163.187.52]) 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 15:16:41 -0500 5, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB02.ad.uky.edu 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 15:16:40 -0500 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 5, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500 5, 24 -- Subject: glutaraldehyde 5, 24 -- Thread-Topic: glutaraldehyde 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw== 5, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu} 5, 24 -- Accept-Language: en-US 5, 24 -- Content-Language: en-US 5, 24 -- X-MS-Has-Attach: 5, 24 -- X-MS-TNEF-Correlator: 5, 24 -- acceptlanguage: en-US 5, 24 -- Content-Type: text/plain; charset="us-ascii" 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12KGgDV025817 ==============================End of - Headers==============================
I've noticed the same thing with a recent box of 2-ml ampoules, but I ran a test batch of cells up and they appear to be fine by TEM. However, I put that lot away to be used for X-gal & SEM (no TEM), just in case there is something horribly wrong with it. I'm currently working off of an older box that we had hidden away.
It will be interesting to see the responses on this one!
Tamara
On Mon, 2 Feb 2009 14:18:04 -0600 mgengle-at-email.uky.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi listers, } We're having an issue with our EM grade glutaraldehyde. } The last three batches of 2ml ampoules I've ordered have } been very viscous and then form a sticky ppt when mixed } with buffer. We have some 10ml ampoules that are fine. } They have different lot numbers which makes me think } it's batch dependent but before I return yet another box } of glut, I was wondering if anyone else has had these } issues. I've also noticed that the glut that isn't so } thick becomes cloudy when first mixed and never has been } before. If anyone has any ideas or has experienced the } same things I'd like to hear from you. } Thank you, } Mary Gail Engle } } } Mary Gail Engle } Sr Research Facility Manager } Electron Microscopy & Imaging Facility } HSRB rm 001 } Ph (859) 323-6108 } FAX (859) 323-8089 } BBSRB rm o74 } Ph (859)323-2701 } FAX (859) 257-1581 } University of KY } Lexington, KY 40536 } } } } } ==============================Original } Headers============================== } 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 } 2009 } 5, 24 -- Received: from ironporta.uky.edu } (ironporta.uky.edu [128.163.184.75]) } 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n12KGgDV025817 } 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb } 2009 14:16:42 -0600 } 5, 24 -- Received: from ex7hb02.ad.uky.edu } ([128.163.187.52]) } 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 } 15:16:41 -0500 } 5, 24 -- Received: from EX7FM03.ad.uky.edu } ([128.163.187.12]) by EX7HB02.ad.uky.edu } 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 } 15:16:40 -0500 } 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} } 5, 24 -- To: "'microscopy-at-microscopy.com'" } {microscopy-at-microscopy.com} } 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500 } 5, 24 -- Subject: glutaraldehyde } 5, 24 -- Thread-Topic: glutaraldehyde } 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw== } 5, 24 -- Message-ID: } {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu} } 5, 24 -- Accept-Language: en-US } 5, 24 -- Content-Language: en-US } 5, 24 -- X-MS-Has-Attach: } 5, 24 -- X-MS-TNEF-Correlator: } 5, 24 -- acceptlanguage: en-US } 5, 24 -- Content-Type: text/plain; charset="us-ascii" } 5, 24 -- MIME-Version: 1.0 } 5, 24 -- Content-Transfer-Encoding: 8bit } 5, 24 -- X-MIME-Autoconverted: from quoted-printable to } 8bit by ns.microscopy.com id n12KGgDV025817 } ==============================End of - } Headers==============================
Have you contacted the vendor? I always found the vendors to be very responsive when I had an issue with a product.
Roger Moretz, Ph.D., ret.
On Mon, Feb 2, 2009 at 3:21 PM, {mgengle-at-email.uky.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi listers, } We're having an issue with our EM grade glutaraldehyde. The last three batches of 2ml ampoules I've ordered have been very viscous and then form a sticky ppt when mixed with buffer. We have some 10ml ampoules that are fine. They have different lot numbers which makes me think it's batch dependent but before I return yet another box of glut, I was wondering if anyone else has had these issues. I've also noticed that the glut that isn't so thick becomes cloudy when first mixed and never has been before. If anyone has any ideas or has experienced the same things I'd like to hear from you. } Thank you, } Mary Gail Engle } } } Mary Gail Engle } Sr Research Facility Manager } Electron Microscopy & Imaging Facility } HSRB rm 001 } Ph (859) 323-6108 } FAX (859) 323-8089 } BBSRB rm o74 } Ph (859)323-2701 } FAX (859) 257-1581 } University of KY } Lexington, KY 40536 } } } } } ==============================Original Headers============================== } 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 2009 } 5, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75]) } 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12KGgDV025817 } 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 14:16:42 -0600 } 5, 24 -- Received: from ex7hb02.ad.uky.edu ([128.163.187.52]) } 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 15:16:41 -0500 } 5, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB02.ad.uky.edu } 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 15:16:40 -0500 } 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} } 5, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500 } 5, 24 -- Subject: glutaraldehyde } 5, 24 -- Thread-Topic: glutaraldehyde } 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw== } 5, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu} } 5, 24 -- Accept-Language: en-US } 5, 24 -- Content-Language: en-US } 5, 24 -- X-MS-Has-Attach: } 5, 24 -- X-MS-TNEF-Correlator: } 5, 24 -- acceptlanguage: en-US } 5, 24 -- Content-Type: text/plain; charset="us-ascii" } 5, 24 -- MIME-Version: 1.0 } 5, 24 -- Content-Transfer-Encoding: 8bit } 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12KGgDV025817 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 35 -- From rcmoretz-at-gmail.com Mon Feb 2 15:02:56 2009 5, 35 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.170]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12L2tBo012298 5, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:02:56 -0600 5, 35 -- Received: by wf-out-1314.google.com with SMTP id 27so1629475wfd.21 5, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 02 Feb 2009 13:02:55 -0800 (PST) 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 35 -- d=gmail.com; s=gamma; 5, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references 5, 35 -- :date:message-id:subject:from:to:content-type 5, 35 -- :content-transfer-encoding; 5, 35 -- bh=WSd1ILRNs0JUKk3BGbDoGZbPQg73jTmj5+G1wpvyER8=; 5, 35 -- b=goXedM+MxU3qv66rPAh4B80DqqjEGWNBG6+tMWeSUs0pFAnvruheILpHxfrUXBB99Q 5, 35 -- MYetJ1iKG4UB5jClAAX49cRW4lo+kMU+Sh8Hprw3szdduERt/8b/MygVRf7edD25cgk7 5, 35 -- 4ccZ6j2i5s/6C0kniNATy6EEow3KPDRaMmngY= 5, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 35 -- d=gmail.com; s=gamma; 5, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 5, 35 -- :content-type:content-transfer-encoding; 5, 35 -- b=jwlmard5D3hDXrPoonRaFJL7JbnnoYpwZQ0INW1lp0pNPLYxKWqpCkuwoHt1JLr5nJ 5, 35 -- pHWhxeOWl+nG7nT5dSRYE4Kz6Jeis2pX9nz3/qdvfPrksv4OeWjpiS5azzQ+5mx03B2G 5, 35 -- trjCE4y+FqKFIKJhhVqxPAAvpqJ/gcXZ6ONvI= 5, 35 -- MIME-Version: 1.0 5, 35 -- Received: by 10.143.160.17 with SMTP id m17mr1972586wfo.298.1233608575351; 5, 35 -- Mon, 02 Feb 2009 13:02:55 -0800 (PST) 5, 35 -- In-Reply-To: {200902022021.n12KLkho032711-at-ns.microscopy.com} 5, 35 -- References: {200902022021.n12KLkho032711-at-ns.microscopy.com} 5, 35 -- Date: Mon, 2 Feb 2009 16:02:55 -0500 5, 35 -- Message-ID: {950e3cfd0902021302x2987fb2ai7ad92b26047598ae-at-mail.gmail.com} 5, 35 -- Subject: Re: [Microscopy] glutaraldehyde 5, 35 -- From: Roger Moretz {rcmoretz-at-gmail.com} 5, 35 -- To: mgengle-at-email.uky.edu, Microscopy Listserv {Microscopy-at-microscopy.com} 5, 35 -- Content-Type: text/plain; charset=ISO-8859-1 5, 35 -- Content-Transfer-Encoding: 8bit 5, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12L2tBo012298 ==============================End of - Headers==============================
In response to some of your comments, these vials are 70% glut, in the sealed ampoules, not opened until ready to use. I've been doing EM since 1971 (oops, telling my age) and have always prepared fix the same way and have never had a problem until now. We keep it in the refrigerator and these last three orders are from 2 weeks ago and maybe 6 months ago so they're fairly new. The vendor was kind enough to replace them but the latest batches are doing the same thing. Like one of the people who wrote, we're hoarding our last good box.
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 Ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 FAX (859) 257-1581 University of KY Lexington, KY 40536
==============================Original Headers============================== 4, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 15:14:07 2009 4, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12LE641004052 4, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:14:07 -0600 4, 24 -- Received: from ex7hb01.ad.uky.edu ([128.163.187.50]) 4, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 16:14:06 -0500 4, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB01.ad.uky.edu 4, 24 -- ([128.163.187.50]) with mapi; Mon, 2 Feb 2009 16:14:03 -0500 4, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 4, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 4, 24 -- Date: Mon, 2 Feb 2009 16:14:03 -0500 4, 24 -- Subject: glutaraldehyde 4, 24 -- Thread-Topic: glutaraldehyde 4, 24 -- Thread-Index: AcmFeysaDIX0dKIGT+6ZNyQVZTbDMg== 4, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D5726-at-EX7FM03.ad.uky.edu} 4, 24 -- Accept-Language: en-US 4, 24 -- Content-Language: en-US 4, 24 -- X-MS-Has-Attach: 4, 24 -- X-MS-TNEF-Correlator: 4, 24 -- acceptlanguage: en-US 4, 24 -- Content-Type: text/plain; charset="us-ascii" 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Transfer-Encoding: 8bit 4, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n12LE641004052 ==============================End of - Headers==============================
From hotdak.netpionavaro2001-at-hotmail.com Mon Feb 2 15:43:05 2009 Return-Path: {hotdak.netpionavaro2001-at-hotmail.com} Received: from google.com (5ad569a1.bb.sky.com [90.213.105.161]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12Lh3sk018553 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 2 Feb 2009 15:43:04 -0600 Received: from [181.56.37.82] (HELO google.com) by busy-gee.cn; Mon, 2 Feb 2009 21:43:03 +0000 Message-ID: {00000007F4216231691959478} Reply-To: Richie Whitehead {wiborg.ken17148-at-gmail.com}
Hi Mary Gail,
We've heard of similar problems with EM grade glutaraldehyde. Based on our experience with glutaraldehyde we think it could be temperature issues during production.
When we started doing glutaraldehyde over 50 years ago we hit a number of snags. It took us several years to get our production down pat.
It's not always easy providing assistance to one's competitor but, because of the importance of this issue, if you have whoever supplied the product call us we'll do what we can.
John Arnott
Disclaimer: Ladd Research supplies EM products including glutaraldehyde
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 15, 25 -- From jd-at-laddresearch.com Mon Feb 2 15:46:02 2009 15, 25 -- Received: from cernan.electric.net (cernan.electric.net [72.35.23.19]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n12Lk2bS018685 15, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:46:02 -0600 15, 25 -- Received: from 1LU6cD-0000S1-VZ by cernan.electric.net with emc1-ok (Exim 4.69) 15, 25 -- (envelope-from {jd-at-laddresearch.com} ) 15, 25 -- id 1LU6cE-0000TP-Td; Mon, 02 Feb 2009 13:46:02 -0800 15, 25 -- Received: by emcmailer; Mon, 02 Feb 2009 13:46:02 -0800 15, 25 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 15, 25 -- by cernan.electric.net with esmtps (TLSv1:AES256-SHA:256) 15, 25 -- (Exim 4.69) 15, 25 -- (envelope-from {jd-at-laddresearch.com} ) 15, 25 -- id 1LU6cD-0000S1-VZ; Mon, 02 Feb 2009 13:46:02 -0800 15, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 25 -- Date: Mon, 02 Feb 2009 16:45:58 -0500 15, 25 -- To: mgengle-at-email.uky.edu 15, 25 -- From: jd {jd-at-laddresearch.com} 15, 25 -- Subject: Re: [Microscopy] glutaraldehyde 15, 25 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 15, 25 -- Mime-Version: 1.0 15, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 25 -- X-Outbound-IP: 216.204.198.170 15, 25 -- X-Env-From: jd-at-laddresearch.com 15, 25 -- X-Virus-Status: Scanned by VirusSMART (c) 15, 25 -- Message-Id: {E1LU6cE-0000TP-Td-at-cernan.electric.net} ==============================End of - Headers==============================
We had this problem too, but solved it by making sure both the concentrated glut. and the buffer it's going into are warmed a little, then no problems mixing them and no precipitate forms when the dilute fixative is put back in the fridge. Not sure of the chemistry behind this, but it is worse when going into cold phosphate buffer rather than cold PIPES buffer. And the viscous stuff in 2 ml ampoules is 70% glut., as mentioned by someone else. We mainly do light microscopy and have not noticed that this fixative does a different job to earlier batches (usually something else is the problem, not the fixative....). cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 3/02/09 8:50 AM, "jd-at-laddresearch.com" {jd-at-laddresearch.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Mary Gail, } } We've heard of similar problems with EM grade glutaraldehyde. Based } on our experience with glutaraldehyde we think it could be } temperature issues during production. } } When we started doing glutaraldehyde over 50 years ago we hit a } number of snags. It took us several years to get our production down pat. } } It's not always easy providing assistance to one's competitor but, } because of the importance of this issue, if you have whoever supplied } the product call us we'll do what we can. } } John Arnott } } Disclaimer: Ladd Research supplies EM products including glutaraldehyde } } Ladd Research } 83 Holly Court } Williston, VT 05495 } } On-line Catalog: www.laddresearch.com } } Telephone: 1-802-658-4961 (anywhere) } Toll Free 1-800-451-3406 (US) } Fax: 1-802-660-8859 } } e-mail: sales-at-laddresearch.com } } At 03:22 PM 2/2/2009, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi listers, } } We're having an issue with our EM grade glutaraldehyde. The last } } three batches of 2ml ampoules I've ordered have been very viscous } } and then form a sticky ppt when mixed with buffer. We have some } } 10ml ampoules that are fine. They have different lot numbers which } } makes me think it's batch dependent but before I return yet another } } box of glut, I was wondering if anyone else has had these } } issues. I've also noticed that the glut that isn't so thick } } becomes cloudy when first mixed and never has been before. If } } anyone has any ideas or has experienced the same things I'd like to } } hear from you. } } Thank you, } } Mary Gail Engle } } } } } } Mary Gail Engle } } Sr Research Facility Manager } } Electron Microscopy & Imaging Facility } } HSRB rm 001 } } Ph (859) 323-6108 } } FAX (859) 323-8089 } } BBSRB rm o74 } } Ph (859)323-2701 } } FAX (859) 257-1581 } } University of KY } } Lexington, KY 40536 } } } } } } } } } } ==============================Original Headers============================== } } 5, 24 -- From mgengle-at-email.uky.edu Mon Feb 2 14:16:42 2009 } } 5, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu } } [128.163.184.75]) } } 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id n12KGgDV025817 } } 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 } } 14:16:42 -0600 } } 5, 24 -- Received: from ex7hb02.ad.uky.edu ([128.163.187.52]) } } 5, 24 -- by ironporta.uky.edu with ESMTP; 02 Feb 2009 15:16:41 -0500 } } 5, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by } } EX7HB02.ad.uky.edu } } 5, 24 -- ([128.163.187.52]) with mapi; Mon, 2 Feb 2009 15:16:40 -0500 } } 5, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} } } 5, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } } 5, 24 -- Date: Mon, 2 Feb 2009 15:16:40 -0500 } } 5, 24 -- Subject: glutaraldehyde } } 5, 24 -- Thread-Topic: glutaraldehyde } } 5, 24 -- Thread-Index: AcmFcyeMqoo+99JuRaeVg183rdKkIw== } } 5, 24 -- Message-ID: } } {DADA8E000C493F4BB3357F27DBF0A85709461D5720-at-EX7FM03.ad.uky.edu} } } 5, 24 -- Accept-Language: en-US } } 5, 24 -- Content-Language: en-US } } 5, 24 -- X-MS-Has-Attach: } } 5, 24 -- X-MS-TNEF-Correlator: } } 5, 24 -- acceptlanguage: en-US } } 5, 24 -- Content-Type: text/plain; charset="us-ascii" } } 5, 24 -- MIME-Version: 1.0 } } 5, 24 -- Content-Transfer-Encoding: 8bit } } 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id n12KGgDV025817 } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 15, 25 -- From jd-at-laddresearch.com Mon Feb 2 15:46:02 2009 } 15, 25 -- Received: from cernan.electric.net (cernan.electric.net } [72.35.23.19]) } 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n12Lk2bS018685 } 15, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 Feb 2009 15:46:02 -0600 } 15, 25 -- Received: from 1LU6cD-0000S1-VZ by cernan.electric.net with emc1-ok } (Exim 4.69) } 15, 25 -- (envelope-from {jd-at-laddresearch.com} ) } 15, 25 -- id 1LU6cE-0000TP-Td; Mon, 02 Feb 2009 13:46:02 -0800 } 15, 25 -- Received: by emcmailer; Mon, 02 Feb 2009 13:46:02 -0800 } 15, 25 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) } 15, 25 -- by cernan.electric.net with esmtps (TLSv1:AES256-SHA:256) } 15, 25 -- (Exim 4.69) } 15, 25 -- (envelope-from {jd-at-laddresearch.com} ) } 15, 25 -- id 1LU6cD-0000S1-VZ; Mon, 02 Feb 2009 13:46:02 -0800 } 15, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 15, 25 -- Date: Mon, 02 Feb 2009 16:45:58 -0500 } 15, 25 -- To: mgengle-at-email.uky.edu } 15, 25 -- From: jd {jd-at-laddresearch.com} } 15, 25 -- Subject: Re: [Microscopy] glutaraldehyde } 15, 25 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} } 15, 25 -- Mime-Version: 1.0 } 15, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 15, 25 -- X-Outbound-IP: 216.204.198.170 } 15, 25 -- X-Env-From: jd-at-laddresearch.com } 15, 25 -- X-Virus-Status: Scanned by VirusSMART (c) } 15, 25 -- Message-Id: {E1LU6cE-0000TP-Td-at-cernan.electric.net} } ==============================End of - Headers==============================
Have a look at a recent paper signed by W. Vanderline in the journal "Microscopy Today" (vol. 16, n° 6, Nov. 2008, p 28-35). A holder for doing STEM-in-SEM is described in details. It is actually sold under license by Ernest Fullam Inc. (items 18300 or 18301) (http://www.fullam.com/Semacces.htm#holders). Your machine shop would be probably inspirited by this holder if you choose a home-made approach which is probably less costly.
I've been having trouble with 3mm/1/8"carbon rods for my Edwards 306 coater since running out of my stash of Union Carbide National Carbon Company "Spectroscopic Electrodes" a while ago.
I don't have a reliably calibrated vacuum gauge in the 306, but I suspect that the vacuum achieved isn't that great, however, it's as good as it was when I was getting great coating from the previous rods.
I bought some "graphite" rods from one supplier, but they needed a higher current and temperature than the 306 could achieve. I then bought some "amorphous carbon" rods, but the success rate isn't very high, there just doesn't seem to be much coating produced.
As I understand it, all rods contain a greater or lesser ratio of graphite and amorphous carbon, and the greater the graphite content, the higher the temperature required for coating.
Does anyone know of a supplier of rods identical or similar to the old Union Carbide National Carbon Company ones?
cheers Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 9, 26 -- From r.sims-at-auckland.ac.nz Tue Feb 3 13:20:40 2009 9, 26 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13JKZ8w012509 9, 26 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Feb 2009 13:20:39 -0600 9, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 385979E9C4 9, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 4 Feb 2009 08:20:34 +1300 (NZDT) 9, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 9, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 9, 26 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 9, 26 -- with ESMTP id YtGqwpgaEG92 for {Microscopy-at-Microscopy.Com} ; 9, 26 -- Wed, 4 Feb 2009 08:20:34 +1300 (NZDT) 9, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1D0AD9E9BE 9, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 4 Feb 2009 08:20:33 +1300 (NZDT) 9, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 9, 26 -- To: Microscopy-at-Microscopy.Com 9, 26 -- Date: Wed, 04 Feb 2009 08:20:33 +1300 9, 26 -- MIME-Version: 1.0 9, 26 -- Subject: Carbon Rods 9, 26 -- Message-ID: {49894FD1.10600.48DDDE-at-r.sims.auckland.ac.nz} 9, 26 -- Priority: normal 9, 26 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 26 -- Content-type: text/plain; charset=US-ASCII 9, 26 -- Content-transfer-encoding: 7BIT 9, 26 -- Content-description: Mail message body ==============================End of - Headers==============================
I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before.
Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems.
So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal.
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 Ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 FAX (859) 257-1581 University of KY Lexington, KY 40536
==============================Original Headers============================== 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55]) 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500 8, 24 -- Subject: glutaraldehyde issue 8, 24 -- Thread-Topic: glutaraldehyde issue 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ== 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu} 8, 24 -- Accept-Language: en-US 8, 24 -- Content-Language: en-US 8, 24 -- X-MS-Has-Attach: 8, 24 -- X-MS-TNEF-Correlator: 8, 24 -- acceptlanguage: en-US 8, 24 -- Content-Type: text/plain; charset="us-ascii" 8, 24 -- MIME-Version: 1.0 8, 24 -- Content-Transfer-Encoding: 8bit 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089 ==============================End of - Headers==============================
I don't know who the vendor is, but I be very reluctant to ever order from them again. It is a terrible thing to ship product when it is known that production issues exist. The time that people waste on compromised fixations and troubleshooting the problem is a huge loss and sometimes the loss is not recoverable. To {not} disclose the vendor leaves many others at risk of wasted time and lost experiments. We pay dearly for what should be trusted chemicals to do our work and vendors shipping bad product really should not be protected from exposure. They know the lot numbers and who received their product and yet I haven't heard that anyone got a notice of recall, or an email to this list to get the word out. We owe it to each other as fellow microscopists to keep the shades open and let in the light.
Dale Callaham
mgengle-at-email.uky.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } } I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before. } } Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems. } } So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal. } } } Mary Gail Engle } Sr Research Facility Manager } Electron Microscopy & Imaging Facility } HSRB rm 001 } Ph (859) 323-6108 } FAX (859) 323-8089 } BBSRB rm o74 } Ph (859)323-2701 } FAX (859) 257-1581 } University of KY } Lexington, KY 40536 } } } } } ==============================Original Headers============================== } 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009 } 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76]) } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089 } 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600 } 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55]) } 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500 } 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu } 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500 } 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} } 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500 } 8, 24 -- Subject: glutaraldehyde issue } 8, 24 -- Thread-Topic: glutaraldehyde issue } 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ== } 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu} } 8, 24 -- Accept-Language: en-US } 8, 24 -- Content-Language: en-US } 8, 24 -- X-MS-Has-Attach: } 8, 24 -- X-MS-TNEF-Correlator: } 8, 24 -- acceptlanguage: en-US } 8, 24 -- Content-Type: text/plain; charset="us-ascii" } 8, 24 -- MIME-Version: 1.0 } 8, 24 -- Content-Transfer-Encoding: 8bit } 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 22 -- From dac-at-research.umass.edu Tue Feb 3 15:02:47 2009 5, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13L2l5q012913 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 15:02:47 -0600 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 5, 22 -- (authenticated bits=0) 5, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13L2kYL022347 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:02:46 -0500 5, 22 -- Message-ID: {4988B151.9070807-at-research.umass.edu} 5, 22 -- Date: Tue, 03 Feb 2009 16:04:17 -0500 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 22 -- Reply-To: dac-at-research.umass.edu 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 5, 22 -- MIME-Version: 1.0 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] glutaraldehyde issue - vendor responsibility 5, 22 -- References: {200902032032.n13KWQXV005914-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200902032032.n13KWQXV005914-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Most times the vendors don't realize there's a problem unless a client lets them know. I have always felt that communication with the vendor is an essential part working in a lab. Alerting the vendor to a potential problem helps them assist you and the rest of their customer base. A simple or single event should not preclude your doing business with them again, nor should it be cause to poison the rest of the microscopy community against them.
Roger Moretz, Ph.D., retired
On Tue, Feb 3, 2009 at 4:07 PM, {dac-at-research.umass.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I don't know who the vendor is, but I be very reluctant to ever order } from them again. It is a terrible thing to ship product when it is known } that production issues exist. The time that people waste on compromised } fixations and troubleshooting the problem is a huge loss and sometimes } the loss is not recoverable. To {not} disclose the vendor leaves many } others at risk of wasted time and lost experiments. We pay dearly for } what should be trusted chemicals to do our work and vendors shipping bad } product really should not be protected from exposure. They know the lot } numbers and who received their product and yet I haven't heard that } anyone got a notice of recall, or an email to this list to get the word } out. We owe it to each other as fellow microscopists to keep the shades } open and let in the light. } } Dale Callaham } } } } mgengle-at-email.uky.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear listers, } } } } I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before. } } } } Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems. } } } } So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal. } } } } } } Mary Gail Engle } } Sr Research Facility Manager } } Electron Microscopy & Imaging Facility } } HSRB rm 001 } } Ph (859) 323-6108 } } FAX (859) 323-8089 } } BBSRB rm o74 } } Ph (859)323-2701 } } FAX (859) 257-1581 } } University of KY } } Lexington, KY 40536 } } } } } } } } } } ==============================Original Headers============================== } } 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009 } } 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76]) } } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089 } } 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600 } } 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55]) } } 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500 } } 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu } } 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500 } } 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} } } 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } } 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500 } } 8, 24 -- Subject: glutaraldehyde issue } } 8, 24 -- Thread-Topic: glutaraldehyde issue } } 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ== } } 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu} } } 8, 24 -- Accept-Language: en-US } } 8, 24 -- Content-Language: en-US } } 8, 24 -- X-MS-Has-Attach: } } 8, 24 -- X-MS-TNEF-Correlator: } } 8, 24 -- acceptlanguage: en-US } } 8, 24 -- Content-Type: text/plain; charset="us-ascii" } } 8, 24 -- MIME-Version: 1.0 } } 8, 24 -- Content-Transfer-Encoding: 8bit } } 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 5, 22 -- From dac-at-research.umass.edu Tue Feb 3 15:02:47 2009 } 5, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13L2l5q012913 } 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 15:02:47 -0600 } 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) } 5, 22 -- (authenticated bits=0) } 5, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13L2kYL022347 } 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:02:46 -0500 } 5, 22 -- Message-ID: {4988B151.9070807-at-research.umass.edu} } 5, 22 -- Date: Tue, 03 Feb 2009 16:04:17 -0500 } 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu} } 5, 22 -- Reply-To: dac-at-research.umass.edu } 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 } 5, 22 -- MIME-Version: 1.0 } 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 5, 22 -- Subject: Re: [Microscopy] glutaraldehyde issue - vendor responsibility } 5, 22 -- References: {200902032032.n13KWQXV005914-at-ns.microscopy.com} } 5, 22 -- In-Reply-To: {200902032032.n13KWQXV005914-at-ns.microscopy.com} } 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 22 -- Content-Transfer-Encoding: 7bit } 5, 22 -- X-Whitelist: TRUE } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 35 -- From rcmoretz-at-gmail.com Tue Feb 3 16:13:38 2009 4, 35 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.175]) 4, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13MDZU2029130 4, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:13:36 -0600 4, 35 -- Received: by wf-out-1314.google.com with SMTP id 27so2169806wfd.21 4, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 03 Feb 2009 14:13:33 -0800 (PST) 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 35 -- d=gmail.com; s=gamma; 4, 35 -- h=domainkey-signature:mime-version:received:in-reply-to:references 4, 35 -- :date:message-id:subject:from:to:content-type 4, 35 -- :content-transfer-encoding; 4, 35 -- bh=/Fowp56ZgMcuY8RW3JjhMlAi6C3YiR4+XopmyD1uzaE=; 4, 35 -- b=RcW80n4jgzhBUD3s0C93fhw4ipc02xDD0ugzcRK4+qxsNTJrb242hrg5+SJxhnGTXT 4, 35 -- BOmph5SmeYelC4zm0XC2emNxIkLwbb6vCccf4iWhDlk9fBN/kWzEdjU8zy3KAfajlzwo 4, 35 -- axQTuw3y4uKkL4J+WmGibrYWZ9vEMmBl1Vzog= 4, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 35 -- d=gmail.com; s=gamma; 4, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 4, 35 -- :content-type:content-transfer-encoding; 4, 35 -- b=MMbSN8pi4bcY6hjjBYfKPSeRm+h/wj32GpjAO2/sw803q2S2BziYUAqxu92ESAbKt2 4, 35 -- uAI8gxDG47WaMqkaNCdLOo4Swjj8eH/4Ts10DQlw3EAQIJ4uuECRGioKX1/kQno9Enoz 4, 35 -- gmR7AqiLwHAJglgSZdxRbMC4tJEHcobHAHZgQ= 4, 35 -- MIME-Version: 1.0 4, 35 -- Received: by 10.142.230.9 with SMTP id c9mr2555530wfh.101.1233699213853; Tue, 4, 35 -- 03 Feb 2009 14:13:33 -0800 (PST) 4, 35 -- In-Reply-To: {200902032107.n13L7LXP019781-at-ns.microscopy.com} 4, 35 -- References: {200902032107.n13L7LXP019781-at-ns.microscopy.com} 4, 35 -- Date: Tue, 3 Feb 2009 17:13:33 -0500 4, 35 -- Message-ID: {950e3cfd0902031413x503535d9h91e0ddf2241cd81d-at-mail.gmail.com} 4, 35 -- Subject: Re: [Microscopy] Re: glutaraldehyde issue - vendor responsibility 4, 35 -- From: Roger Moretz {rcmoretz-at-gmail.com} 4, 35 -- To: dac-at-research.umass.edu, Microscopy Listserv {Microscopy-at-microscopy.com} 4, 35 -- Content-Type: text/plain; charset=ISO-8859-1 4, 35 -- Content-Transfer-Encoding: 8bit 4, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13MDZU2029130 ==============================End of - Headers==============================
I agree with you completely. I think the vendor should be the first one contacted in the event of a problem, before the list. But where Mary Gail said they were aware they had production problems and had still shipped product with no warnings - hadn't let her know, or anyone else most likely, this is a breach of trust to their consumers and clearly has led to some compromised work and wasted time. I would lose trust in such a vendor. I don't think it is good enough to ship product and hope it works out and wait for the "field testers" to complain; glutaraldehyde is a chemical and can be tested and in the case that anything seems wrong it shouldn't be shipped to unsuspecting users. That isn't the way to build trust.
Dale
Roger Moretz wrote: } Most times the vendors don't realize there's a problem unless a client } lets them know. I have always felt that communication with the vendor } is an essential part working in a lab. Alerting the vendor to a } potential problem helps them assist you and the rest of their customer } base. A simple or single event should not preclude your doing } business with them again, nor should it be cause to poison the rest of } the microscopy community against them. } } Roger Moretz, Ph.D., retired } } On Tue, Feb 3, 2009 at 4:07 PM, {dac-at-research.umass.edu} wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } I don't know who the vendor is, but I be very reluctant to ever order } } from them again. It is a terrible thing to ship product when it is known } } that production issues exist. The time that people waste on compromised } } fixations and troubleshooting the problem is a huge loss and sometimes } } the loss is not recoverable. To {not} disclose the vendor leaves many } } others at risk of wasted time and lost experiments. We pay dearly for } } what should be trusted chemicals to do our work and vendors shipping bad } } product really should not be protected from exposure. They know the lot } } numbers and who received their product and yet I haven't heard that } } anyone got a notice of recall, or an email to this list to get the word } } out. We owe it to each other as fellow microscopists to keep the shades } } open and let in the light. } } } } Dale Callaham } } } } } } } } mgengle-at-email.uky.edu wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Dear listers, } } } } } } I just wanted to thank all of you for your input regarding the glutaraldehyde problem. The vendor said they'd been having problems with the 70% in particular which is what I've always used, and she sent me some newly made glut. Evidently it was a manufacturing problem. It's the correct semi-viscous consistency now and mixes very well with the cold buffer with no clouds or gummy ppt as before. } } } } } } Someone suggested leaving it out for a few days before using it but I'm not sure that's a good idea. I have always used freshly opened vials the day of fixation for many years with no problems. } } } } } } So I would suggest if any of you have new developments with your glut while employing your usual protocols, you send it back and get new vials. I didn't realize that the vendors package their own glutaraldehyde but evidently they do, so the problems aren't necessarily universal. } } } } } } } } } Mary Gail Engle } } } Sr Research Facility Manager } } } Electron Microscopy & Imaging Facility } } } HSRB rm 001 } } } Ph (859) 323-6108 } } } FAX (859) 323-8089 } } } BBSRB rm o74 } } } Ph (859)323-2701 } } } FAX (859) 257-1581 } } } University of KY } } } Lexington, KY 40536 } } } } } } } } } } } } } } } ==============================Original Headers============================== } } } 8, 24 -- From mgengle-at-email.uky.edu Tue Feb 3 14:26:50 2009 } } } 8, 24 -- Received: from ironportb.uky.edu (ironportb.uky.edu [128.163.184.76]) } } } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13KQnFV030089 } } } 8, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 14:26:50 -0600 } } } 8, 24 -- Received: from ex7hb03.ad.uky.edu ([128.163.187.55]) } } } 8, 24 -- by ironportb.uky.edu with ESMTP; 03 Feb 2009 15:26:49 -0500 } } } 8, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu } } } 8, 24 -- ([128.163.187.55]) with mapi; Tue, 3 Feb 2009 15:26:48 -0500 } } } 8, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} } } } 8, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } } } 8, 24 -- Date: Tue, 3 Feb 2009 15:26:47 -0500 } } } 8, 24 -- Subject: glutaraldehyde issue } } } 8, 24 -- Thread-Topic: glutaraldehyde issue } } } 8, 24 -- Thread-Index: AcmGPbzas4WjkGzHR4KirgFOyFdHRQ== } } } 8, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A85709461D573C-at-EX7FM03.ad.uky.edu} } } } 8, 24 -- Accept-Language: en-US } } } 8, 24 -- Content-Language: en-US } } } 8, 24 -- X-MS-Has-Attach: } } } 8, 24 -- X-MS-TNEF-Correlator: } } } 8, 24 -- acceptlanguage: en-US } } } 8, 24 -- Content-Type: text/plain; charset="us-ascii" } } } 8, 24 -- MIME-Version: 1.0 } } } 8, 24 -- Content-Transfer-Encoding: 8bit } } } 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n13KQnFV030089 } } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } } 5, 22 -- From dac-at-research.umass.edu Tue Feb 3 15:02:47 2009 } } 5, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) } } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13L2l5q012913 } } 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 15:02:47 -0600 } } 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) } } 5, 22 -- (authenticated bits=0) } } 5, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13L2kYL022347 } } 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } } 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:02:46 -0500 } } 5, 22 -- Message-ID: {4988B151.9070807-at-research.umass.edu} } } 5, 22 -- Date: Tue, 03 Feb 2009 16:04:17 -0500 } } 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu} } } 5, 22 -- Reply-To: dac-at-research.umass.edu } } 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 } } 5, 22 -- MIME-Version: 1.0 } } 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } 5, 22 -- Subject: Re: [Microscopy] glutaraldehyde issue - vendor responsibility } } 5, 22 -- References: {200902032032.n13KWQXV005914-at-ns.microscopy.com} } } 5, 22 -- In-Reply-To: {200902032032.n13KWQXV005914-at-ns.microscopy.com} } } 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 5, 22 -- Content-Transfer-Encoding: 7bit } } 5, 22 -- X-Whitelist: TRUE } } ==============================End of - Headers============================== } }
==============================Original Headers============================== 4, 22 -- From dac-at-research.umass.edu Tue Feb 3 16:56:52 2009 4, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n13MuqBY011702 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 16:56:52 -0600 4, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 4, 22 -- (authenticated bits=0) 4, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n13Mup43030634 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 17:56:51 -0500 4, 22 -- Message-ID: {4988CC0E.3060201-at-research.umass.edu} 4, 22 -- Date: Tue, 03 Feb 2009 17:58:22 -0500 4, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 22 -- Reply-To: dac-at-research.umass.edu 4, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 4, 22 -- MIME-Version: 1.0 4, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] Re: glutaraldehyde issue - vendor responsibility 4, 22 -- References: {200902032107.n13L7LXP019781-at-ns.microscopy.com} {950e3cfd0902031413x503535d9h91e0ddf2241cd81d-at-mail.gmail.com} 4, 22 -- In-Reply-To: {950e3cfd0902031413x503535d9h91e0ddf2241cd81d-at-mail.gmail.com} 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
I'd like to buy a broken vertical engage scanner for the NanoScope Multimode AFM: --Model EV or JV scanner, broken or working, whether or not it is labeled with a "V".
A vertical engage scanner has a single screw at its base (and built-in springs on its sides), as shown in this photo. http://www.asmicro.com/images/4576-EV-Vertical%20Engage%20AFM%20Scanner%20LR.JPG
In contrast, an ordinary scanner has 3 screws at its base (2 adjusted manually, one coupled to the stepper motor) and uses separate springs to secure the optical head.
Please contact me offline for further details. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 5, 24 -- From donc-at-asmicro.com Tue Feb 3 22:27:59 2009 5, 24 -- Received: from smtp108.sbc.mail.re2.yahoo.com (smtp108.sbc.mail.re2.yahoo.com [68.142.229.97]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n144RvHP005290 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 3 Feb 2009 22:27:58 -0600 5, 24 -- Received: (qmail 17032 invoked from network); 4 Feb 2009 04:27:56 -0000 5, 24 -- Received: from unknown (HELO asm15) (donc-at-68.51.122.238 with login) 5, 24 -- by smtp108.sbc.mail.re2.yahoo.com with SMTP; 4 Feb 2009 04:27:56 -0000 5, 24 -- X-YMail-OSG: ApE4jYkVM1mmv2YSjBsMmiJ04u74fsSfLl_N4bbhSin8xjYYAUQqyow_.Our4D0R8vR4KZr6qdNFCveiwnh4Vjn4hheoIYm_ENHCTBLb7CXT02fD0AmBqp1VdXfzavlpjhAaqz.gmRVqHT56Qa8gdQyQxjvM3RnTULuS08jVvZBqD8kQc2e1waxRUGo1QBoRAcxlsxsRENsEoLgZjU5m53NA4PqrgPaoL2_oe4ZUh4zHwA-- 5, 24 -- X-Yahoo-Newman-Property: ymail-3 5, 24 -- Message-ID: {A8754912B42A434BB5B7245AE2A34716-at-asm15} 5, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 5, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 5, 24 -- Subject: AFM - Broken Multimode AFM Scanner wanted 5, 24 -- Date: Tue, 3 Feb 2009 23:27:43 -0500 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- format=flowed; 5, 24 -- charset="iso-8859-1"; 5, 24 -- reply-type=original 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Priority: 3 5, 24 -- X-MSMail-Priority: Normal 5, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
I really have to join Roger in this. I started in EM about 2 years before Mary Gail Engle. While most of my work is now gastroenteric virology, EM is still part of the job description. In all this time I have yet to find a vendor who did not view their responsibility to the community very seriously. In fact, the only problem I've ever seen has to do with Kodak's decision to continue providing paper and chemistry, but that is a corporate decision I can at least understand, if not endorse.
Don't tar and feather the vendor for one event.
Paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Feb 4 08:10:58 2009 6, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n14EAwTs021341 6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 08:10:58 -0600 6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 6, 20 -- (authenticated bits=0) 6, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n14EAuAw006486; 6, 20 -- Wed, 4 Feb 2009 08:10:56 -0600 (CST) 6, 20 -- Message-ID: {4989A1F0.5030208-at-umanitoba.ca} 6, 20 -- Date: Wed, 04 Feb 2009 08:10:56 -0600 6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: dac-at-research.umass.edu 6, 20 -- Subject: Re: [Microscopy] Re: glutaraldehyde issue - vendor responsibility 6, 20 -- References: {200902032104.n13L4dRO015834-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200902032104.n13L4dRO015834-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
} 2. Neither one does single side blotting (nicely, if possible).
Dear Chen Xu, Blotting one face of the grid is not easy on the Vitrobot, but blotting from the lower edge can be done by putting a piece of filter paper in a reverse-action tweezer and inserting it through the side port. This method can be used whenever one-sided blotting is necessary, and it is even better than one-sided blotting if the specimen binds to filter paper better than it does to the surface of the grid. I am pretty sure this technique can be used with the CP3 as well--I only saw one at M&M, so I don't have any personal experience with it. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
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Email: dkloos-at-parallaxray.com Name: Don Kloos
Organization: Parallax Research, Inc.
Title-Subject: [Filtered] Parallax Research is looking for reps to hire
Question: Hello All:
I need your help. Parallax Research is seeking to hire manufacturer's reps and distributors to handle its new EDS and WDS x-ray microanalysis products in North America, Europe, Asia, and other regions of the world. If you know of an individual or company in your area that you like to deal with and can recommend them, please let me know.
Thank you for your time.
Don Kloos Parallax Research. Email: dkloos-at-parallaxray.com. Phone: 1 866 581-XRAY, or +1 714 897-9779. www.parallaxray.com
We were analyzing some samples of tin solder with varying but small amounts of lead. The solder was electroplated as a film (~7-10µm) on copper. We need to know % of lead in the samples with reasonable accuracy. The amounts of Pb varied from about 0.5 to 3% based on information from another quantifying technique.
We collected a spectrum for 120sec using 30kV (3000-5000cps) in an area and then repeated this on the same area two other times under identical conditions and immediately following the previous spectrum. Each time the ratio of Sn to Pb varied significantly. We are at a loss to explain why there was such a difference in the ratios from the identical area. Could this be due to something going on due to the repeated sampling of the same area?
Any idea as to why we had such poor reproducibility?
Thanks, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 12, 30 -- From dsherman-at-purdue.edu Wed Feb 4 21:07:58 2009 12, 30 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1537wD4026640 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:07:58 -0600 12, 30 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 12, 30 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n1537wpL022381 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 12, 30 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n1537woL011609 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 12, 30 -- Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 12, 30 -- Thu, 5 Feb 2009 03:07:50 +0000 12, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119 12, 30 -- Date: Wed, 04 Feb 2009 22:07:49 -0500 12, 30 -- Subject: Puzzling EDX results 12, 30 -- From: Debby Sherman {dsherman-at-purdue.edu} 12, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 12, 30 -- Message-ID: {C5AFC235.2672D%dsherman-at-purdue.edu} 12, 30 -- Thread-Topic: Puzzling EDX results 12, 30 -- Thread-Index: AcmHPuz1HKZ2V9XNDUeJmziVuCJ6lA== 12, 30 -- Mime-version: 1.0 12, 30 -- Content-type: text/plain; 12, 30 -- charset="ISO-8859-1" 12, 30 -- X-OriginalArrivalTime: 05 Feb 2009 03:07:58.0683 (UTC) FILETIME=[F2BAF2B0:01C9873E] 12, 30 -- X-PMX-Version: 5.4.0.320885 12, 30 -- X-PerlMx-Virus-Scanned: Yes 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1537wD4026640 ==============================End of - Headers==============================
I had many years experience analyzing electroplated SnPb solders on Cu using table-top micro-xrf instruments (Seiko, Fischer, CMI, Thermo, MXRF units). It's obviously not quite the same as SEM/EDS in terms of excitation, but here's some ideas that may help:
1) Sn Ka and Pb La intensities vary with solder thickness AND composition. If your sample is not infinite with respect to these energies, you will get varying results depending on where you measure (or what angle). In your case, the layer is almost all Sn, so the film infinite thickness is about 50um or more with respect to Sn Ka. 2) If you're using Sn La and Pb Ma (or even Pb La to a lesser extent) then the film infinite thickness is much lower. I'm not sure what lines you are using, but you are accelerating at 30KV, enough to excite Sn Ka. 3) Solder is notoriously non-uniform in plated and hot-flow processes. I found in studies that the composition varied with depth as well. In addition, an intermetallic Sn-Cu layer begins forming at the Sn-Cu layer interface which can affect results, especially for thinner layers. Are you measuring EXACTLY the same area and getting drastically different results? 4) If it is just reproducibility, what are your net countrates for Sn and Pb? Peak/background? 5) In SnPb/Cu on circuit boards, the presence of Br in the FR4 / G10 substrates interfered with Pb La considerably and somewhat with Pb Ma. Consider the interference from Br. 6) How are you quantifying the composition and thickness? (They're related unless you're measuring an 'infinitely thick' layer and/or using the lower energy lines.)
Call me if you want to talk.
Regards,
Don
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, February 04, 2009 7:17 PM To: dkloos-at-parallaxray.com
Hi all,
We were analyzing some samples of tin solder with varying but small amounts of lead. The solder was electroplated as a film (~7-10µm) on copper. We need to know % of lead in the samples with reasonable accuracy. The amounts of Pb varied from about 0.5 to 3% based on information from another quantifying technique.
We collected a spectrum for 120sec using 30kV (3000-5000cps) in an area and then repeated this on the same area two other times under identical conditions and immediately following the previous spectrum. Each time the ratio of Sn to Pb varied significantly. We are at a loss to explain why there was such a difference in the ratios from the identical area. Could this be due to something going on due to the repeated sampling of the same area?
Any idea as to why we had such poor reproducibility?
Thanks, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 12, 30 -- From dsherman-at-purdue.edu Wed Feb 4 21:07:58 2009 12, 30 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1537wD4026640 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:07:58 -0600 12, 30 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 12, 30 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n1537wpL022381 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 12, 30 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n1537woL011609 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 12, 30 -- Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 12, 30 -- Thu, 5 Feb 2009 03:07:50 +0000 12, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119 12, 30 -- Date: Wed, 04 Feb 2009 22:07:49 -0500 12, 30 -- Subject: Puzzling EDX results 12, 30 -- From: Debby Sherman {dsherman-at-purdue.edu} 12, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 12, 30 -- Message-ID: {C5AFC235.2672D%dsherman-at-purdue.edu} 12, 30 -- Thread-Topic: Puzzling EDX results 12, 30 -- Thread-Index: AcmHPuz1HKZ2V9XNDUeJmziVuCJ6lA== 12, 30 -- Mime-version: 1.0 12, 30 -- Content-type: text/plain; 12, 30 -- charset="ISO-8859-1" 12, 30 -- X-OriginalArrivalTime: 05 Feb 2009 03:07:58.0683 (UTC) FILETIME=[F2BAF2B0:01C9873E] 12, 30 -- X-PMX-Version: 5.4.0.320885 12, 30 -- X-PerlMx-Virus-Scanned: Yes 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1537wD4026640 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 31 -- From dkloos-at-parallaxray.com Wed Feb 4 22:05:22 2009 26, 31 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) 26, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1545LaG009350 26, 31 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:05:21 -0600 26, 31 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) 26, 31 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) 26, 31 -- (envelope-from {dkloos-at-parallaxray.com} ) 26, 31 -- id 1LUvUa-0005QY-JR; Wed, 04 Feb 2009 21:05:32 -0700 26, 31 -- Reply-To: {dkloos-at-parallaxray.com} 26, 31 -- From: "Don Kloos" {dkloos-at-parallaxray.com} 26, 31 -- To: {dsherman-at-purdue.edu} 26, 31 -- Cc: {microscopy-at-microscopy.com} 26, 31 -- References: {200902050316.n153GpuM007423-at-ns.microscopy.com} 26, 31 -- Subject: RE: [Microscopy] Puzzling EDX results 26, 31 -- Date: Wed, 4 Feb 2009 20:05:16 -0800 26, 31 -- Organization: Parallax Research 26, 31 -- Message-ID: {4EE6E5D005D146CF99708D3736DE3B3E-at-donl} 26, 31 -- MIME-Version: 1.0 26, 31 -- Content-Type: text/plain; 26, 31 -- charset="iso-8859-1" 26, 31 -- X-Mailer: Microsoft Office Outlook 11 26, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 26, 31 -- Thread-Index: AcmHQDcr3qoMYnyrTO2bXd1E+DCaogAA1A+g 26, 31 -- In-Reply-To: {200902050316.n153GpuM007423-at-ns.microscopy.com} 26, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 26, 31 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com 26, 31 -- X-AntiAbuse: Original Domain - microscopy.com 26, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] 26, 31 -- X-AntiAbuse: Sender Address Domain - parallaxray.com 26, 31 -- Content-Transfer-Encoding: 8bit 26, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1545LaG009350 ==============================End of - Headers==============================
What was the morphology of the analyzed area? Spikes of Sn will change after being whacked by a 30 KV beam. Why did you use 30KV? For all Ma peaks, 5-6KV out to do the job. You could double check this at 20KV for La peaks.
The geometry of the specimen will make a difference and so will the effects of being scanned. Did the scanned areas show polymerization rectangles? If so, that pushes up H, C and O. So all other elements degrade in %. Of course not due to EDS non-detected H.
I would suggest trying 6KV, then 10KV and finally 15KV. See what you get. Did you detect any Cu? Is the sample surely grounded? Is the probe current near the same from one collection to another? If not, why not? That will make a big difference.
If I did not say so here, I have to build a spectra for special specimens that start at 3KV and wind up at 20KV. This also assumes that your specimen is at the EDS analytical WD.
Can you supply a sample for off-site comparison? I'm working on Pb and Pb-free components (mostly ICs). The RHOS thing is somewhat nebulous. IMO, definitely not precise.
gary g.
At 07:10 PM 2/4/2009, you wrote:
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So, I thought I sent the following message to the list, but it never showed up. I looked at again and wondered if I should resend it, at first I wasn't going to do it, but then I decided, why not. After reviewing it I thought it might be a little naive, but I am curious about what you think.
Here it is:
Greetings
At the risk of being labeled a heretic, I'd like to know the current state of biological SEM.
Here's the deal, I am teaching a class on bio SEM and just sat down to prepare a lecture on modern biological SEM. I have lots of nice books with cool SEM pictures, but I noticed they are all about 20 years old. Heck, I even did some nice bio SEM, but that was, well, 20 years ago.
I tried to cruise the web to see if I could get up to date on what's happening, but web access to journals from here is pretty lame. So is the offering of ultrastructural type journals at our library, like, there aren't any.
I do get a few journals and I can access a few on the web, but most of the papers in the searchable index from JCB etc. that have SEM are, well, pretty old. Is there much modern research using SEM being done in biology these days? Mostly I see the SEM being applied to materials type research. Not that the biology work that has been done is not elegant, it just seems like a lot of it is finding its way on to journal covers or 'coffee table' picture books after being colorized in Photoshop.
I am OK with that, and if the kind of SEM that I know, simple fix, dehydr, and CPD is the state of the art, then I will feel better getting my students to do these things. But if there are sources for something new, I would like to be able to tell them about it. I mean, after all,how many fly eyes, bee's knees, and pollen grains can you look at?
Out on a limb, and with my asbestos suit fully zipped,
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n155A6nj005730 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07 -0600 14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 14, 42 -- by localhost (Email Security Appliance) with SMTP id 221DF1BA6DD_98A6F4BB 14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000 (GMT) 14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 14, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 177F01BA6D5_98A6F4BF 14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000 (GMT) 14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 14, 42 -- with ESMTP id 45413886 for microscopy-at-microscopy.com; Wed, 04 Feb 2009 21:10:01 -0800 14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 14, 42 -- ESMTP id KEKTM900.61V for {microscopy-at-microscopy.com} ; Wed, 4 14, 42 -- Feb 2009 20:54:09 -0800 14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 648B68F74E48 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:01 -0800 (PST) 14, 42 -- X-Virus-Scanned: amavisd-new at 14, 42 -- X-Spam-Flag: NO 14, 42 -- X-Spam-Score: -2.499 14, 42 -- X-Spam-Level: 14, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] 14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 14, 42 -- with ESMTP id VKwKzh5c2Zca for {microscopy-at-microscopy.com} ; 14, 42 -- Wed, 4 Feb 2009 21:10:01 -0800 (PST) 14, 42 -- Received: from [172.20.3.16] (unknown [172.20.3.16]) 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 007508F74E1E 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:00 -0800 (PST) 14, 42 -- Message-Id: {A7C0E8F7-7669-4A7A-A038-0F6A54694846-at-deltacollege.edu} 14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 14, 42 -- To: microscopy-at-microscopy.com 14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 42 -- Content-Transfer-Encoding: 7bit 14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 14, 42 -- Subject: Current biological SEM 14, 42 -- Date: Wed, 4 Feb 2009 21:10:00 -0800 14, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Jonathan, You ask a relevant question. As a biologist who has gotten deeply into SEM in the last decade, I am surprised that there is not more use on our side of the fence. I suspect that most of the recent advances in SEM tech have been driven my materials folks and that the biologists simply don't know about them. Let me give you my perspective on where I think the advances are.
1. Environmental SEM. It is now possible to examine a fully hydrated specimen in the SEM. The sample needs to be frozen or at least cold to minimize evaporation but otherwise not processed at all. I am aware of people who use ESEM to study root hairs and also floral meristems. I suspect animal scientists use also. The point is that fixation crtical point dry and coating are all avoided and instead the sample is directly viewed. The drawback is that once the sample is out of the machine it cannot be viewed again later. And the resolution of an ESEM is no better than a conventional tungsten filament model.
2. Wet chambers. A rather recent (5 years or so?) development are thin membranes that the beam can pass but gas cannot. This allows fully hydrated samples to be viewed at ambient temp and pressure in chambers built with the top surface being that special membrane. Thus living cells can be grown on the membrane (inside the membrane) and viewed in the SEM while they are alive. It is of course a question how much radiation damage the beam will induce. Still the cells are certanly viable for a while. I have seen papers using this tech on animal tissue culture cells. There was quite a lot of excitement when these chambers hit the market but I don't know how successful they have proven in the "real" world.
The above points are geared for eliminating fixation and looking at samples as close to living as possible. This is clearly difficult given the fundamental high-vacuum nature of the SEM beast. The other direction where advances lay is in the high vac, high resolution end of things. Here the advances in SEM design have been phenomenal. The field emission gun allows resolution to be obtained that is almost as good as the best TEMs and certainly far beyond what the good old fly eye SEM was capable of seeing.
3. High resolution. With the field emission gun, macromolecules can be resolved. I among others have taken advantage of this to study the ultrastructure of the plant cell wall. Cellulose microfibrils are on order of 10 nm and these can be readily imaged with FESEM. What is particularly useful for me is that I can put my plant stem 1 mm by 10 mm in to instrument, get good low mag survey views and crank up the magnficiation to the ultrastructual level in any cell on the sample that is appropriate. The easy moving between low and high magnifications is extremely helpful where extensive sampling is needed or when rare features need to be found. Other biological systems that have been profitably studied with FESEM include the cytoskeleton, bacterial biofilms, biomineralization, and nuclear pore complexes. All of these cases involve complex three dimensional structures and the FESEM lets you image them at high resolution in the intact (or semi-intact) state.
4. Low voltage. Along with the sharper probe of the FESEM comes the ability to work at really low accelerating voltage. In the recent vintage FESEM's it is easy to image at 1 kV and possible to go down even as low as a tenth of that. This has several advantages. First, many samples are destroyed or damaged at 30 kV or even at 10 kV. Second, the lower the beam voltage the more exclusively the image comes from the surface. This increases the resolution in the depth direction.
Both low voltage and the smaller probe size allow much thinner metal coats to be applied, and in some cases no coat at all. For the work I have been doing on the cell wall, I use ca. 1 to 2 nm of Pt. This is an order of magnitude less than typical gold coats for conventional tungsten SEM work.
5. Backscattered imaging. Of particular importance to biologists is being able to image backscattered electrons. This allows the localization of gold probes, as in gold conjugated secondary antibodies. With the latest instrumentation, one can colect electrons from selected energy (or angle) levels and mix signals from several detectors. Thus, the SE detector can show the topography of the sample while the BSE detector can pick up the signal from the gold and the two are overlain seamlessly. The sensitivity of the detectors is such that the gold can be imaged at high contrast even with a sample lightly covered with platinum. I took advantage of this (with slightly older not quite so performant technology) to detect gamma tubulin in the plant cell cortex. Others have localized antigens on the plasma membrane and cell wall, to name a few.
There have been parallel developments in elemental analysis (EDS and related modes) and I expect biologists have taken advantage of these too but it is not my area so I don't know. Perhaps a helpful netizen will chime in.
Summarizing, you are right, recent use of SEM in biology is a little bit burried. However these uses will be well worth your digging up. Good luck with your course, Tobias
} } } Greetings } } At the risk of being labeled a heretic, I'd like to know the current } state of biological SEM. } } Here's the deal, I am teaching a class on bio SEM and just sat down to } prepare a lecture on modern biological SEM. I have lots of nice books } with cool SEM pictures, but I noticed they are all about 20 years old. } Heck, I even did some nice bio SEM, but that was, well, 20 years ago. } } I tried to cruise the web to see if I could get up to date on what's } happening, but web access to journals from here is pretty lame. So is } the offering of ultrastructural type journals at our library, like, } there aren't any. } } I do get a few journals and I can access a few on the web, but most of } the papers in the searchable index from JCB etc. that have SEM are, } well, pretty old. Is there much modern research using SEM being done } in biology these days? Mostly I see the SEM being applied to materials } type research. Not that the biology work that has been done is not } elegant, it just seems like a lot of it is finding its way on to } journal covers or 'coffee table' picture books after being colorized } in Photoshop. } } I am OK with that, and if the kind of SEM that I know, simple fix, } dehydr, and CPD is the state of the art, then I will feel better } getting my students to do these things. But if there are sources for } something new, I would like to be able to tell them about it. I mean, } after all,how many fly eyes, bee's knees, and pollen grains can you } look at? } } Out on a limb, and with my asbestos suit fully zipped, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } }
==============================Original Headers============================== 12, 21 -- From baskin-at-bio.umass.edu Thu Feb 5 02:05:58 2009 12, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1585wa3024378 12, 21 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 02:05:58 -0600 12, 21 -- Received: from [133.48.48.239] (dh48-239.nibb.ac.jp [133.48.48.239]) 12, 21 -- (authenticated bits=0) 12, 21 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id n1585pIE020660 12, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 12, 21 -- Thu, 5 Feb 2009 03:05:54 -0500 (EST) 12, 21 -- Mime-Version: 1.0 12, 21 -- Message-Id: {p06240538c5b0451e1d83-at-[133.48.48.239]} 12, 21 -- In-Reply-To: {200902050510.n155AuFl006549-at-ns.microscopy.com} 12, 21 -- References: {200902050510.n155AuFl006549-at-ns.microscopy.com} 12, 21 -- Date: Thu, 5 Feb 2009 17:05:50 +0900 12, 21 -- To: jkrupp-at-deltacollege.edu 12, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 12, 21 -- Subject: Re: [Microscopy] Current biological SEM 12, 21 -- Cc: microscopy-at-microscopy.com 12, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 12, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Thu, 05 Feb 2009 03:05:56 -0500 (EST) 12, 21 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19 ==============================End of - Headers==============================
From felo-at-chello.pl Thu Feb 5 05:22:56 2009 Return-Path: {felo-at-chello.pl} Received: from google.com (dhcp-077-250-106-149.chello.nl [77.250.106.149]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15BMtEa030198 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 5 Feb 2009 05:22:55 -0600 Received: from [118.162.44.132] (HELO google.com) by luckybuyadrink.com; Thu, 5 Feb 2009 12:20:53 +0530
Jon,
A quick answer: "Biological Low-Voltage Scanning Electron Microscopy", H. Schatten and j. Pawley, eds. Springer, 2008. Lots of good information in there. The major advances in Biological SEM have been in cryo methods and high resolution (which pretty much means low voltage) methds. SEM using gold-conjugated antibodies and other ligands are being done. There are also environmental SEM and fluid-chamber SEM using backscattered detectors. Correlation studies of e.g. gold-conjugated primary antibodies followed by fluorescently labeled secondaries should (I hope) become more common. I suspect most of the advances are in the primary literature, but not necessarily in the microscopy journals. Rather, in journals like "Immunity", etc. Then again, one reason old texts are still good is that there isn't necessarily a need for new methods, "just" new studies.
Phil
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==============================Original Headers============================== 5, 25 -- From oshel1pe-at-cmich.edu Thu Feb 5 07:17:29 2009 5, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15DHTsk001733 5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 07:17:29 -0600 5, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n15DHNNo013319 5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:17:25 -0500 5, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 5, 25 -- Thu, 5 Feb 2009 08:16:54 -0500 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {f06240800c5b0957767e7-at-[141.209.160.249]} 5, 25 -- In-Reply-To: {200902050513.n155DH5n011616-at-ns.microscopy.com} 5, 25 -- References: {200902050513.n155DH5n011616-at-ns.microscopy.com} 5, 25 -- Date: Thu, 5 Feb 2009 08:16:51 -0500 5, 25 -- To: Microscopy-at-microscopy.com 5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 25 -- Subject: Re: [Microscopy] Current biological SEM 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-OriginalArrivalTime: 05 Feb 2009 13:16:54.0390 (UTC) FILETIME=[03B56960:01C98794] 5, 25 -- X-Canit-CHI2: 0.00 5, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 25 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 5, 25 -- X-CanItPRO-Stream: default 5, 25 -- X-Canit-Stats-ID: 8474343 - 8f4ddedec40c 5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Listservers, What concentration of Hepes or Pipes would you use for buffering a standard TEM fixation (and subsequent washes), 0.1M, 50mM or 20 mM? Thanks for all responses.
Michael Delannoy
==============================Original Headers============================== 2, 27 -- From delannoy-at-jhmi.edu Thu Feb 5 08:19:50 2009 2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EJmkX017907 2, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:19:50 -0600 2, 27 -- X-IronPort-AV: E=Sophos;i="4.37,385,1231131600"; 2, 27 -- d="scan'208";a="186662157" 2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 05 Feb 2009 09:20:03 -0500 2, 27 -- Received: from johnshopkins.edu ([10.181.31.211]) by jesmail.johnshopkins.edu 2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 2, 27 -- with ESMTP id {0KEL00JV0JTEKC10-at-jesmail.johnshopkins.edu} for 2, 27 -- microscopy-at-microscopy.com; Thu, 05 Feb 2009 09:20:02 -0500 (EST) 2, 27 -- Received: from [10.181.192.191] (Forwarded-For: [10.16.66.59]) 2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Thu, 05 Feb 2009 09:20:02 -0500 2, 27 -- Date: Thu, 05 Feb 2009 09:20:02 -0500 2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 27 -- Subject: Hepes OR Pipes 2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 27 -- Message-id: {fc16d6674f2c.498aaf42-at-johnshopkins.edu} 2, 27 -- MIME-version: 1.0 2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 2, 27 -- Content-type: text/plain; charset=us-ascii 2, 27 -- Content-language: en 2, 27 -- Content-transfer-encoding: 7BIT 2, 27 -- Content-disposition: inline 2, 27 -- X-Accept-Language: en 2, 27 -- Priority: normal ==============================End of - Headers==============================
Our lab deals with Dental biomaterials and we have an SEM. The biological part comes into play for tissue engineering, e.g. seeing if cells are in scaffolds, are they happy with the surface they are on, etc. We've also looked at bacteria in microleakage studies. Some of these problems are unsolved but improved upon in 20 years. From what I've seen of research, the more diverse the background you have, the more apt you are to come up with a better solution to a problem. Remember Edison, the inventor of the phonograph and practical light bulb? He had to come up with a complete system to make the light bulb work outside of the lab. He studied how the gas company distributed gas, an already tried and true system. And the woman who invented the circular saw blade did so because she was using a spinning wheel while the males of her family were pushing and pulling a saw through wood. It's connections of old and new.
Ron L
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Thursday, February 05, 2009 12:13 AM To: lherault-at-bu.edu
So, I thought I sent the following message to the list, but it never showed up. I looked at again and wondered if I should resend it, at first I wasn't going to do it, but then I decided, why not. After reviewing it I thought it might be a little naive, but I am curious about what you think.
Here it is:
Greetings
At the risk of being labeled a heretic, I'd like to know the current state of biological SEM.
Here's the deal, I am teaching a class on bio SEM and just sat down to prepare a lecture on modern biological SEM. I have lots of nice books with cool SEM pictures, but I noticed they are all about 20 years old. Heck, I even did some nice bio SEM, but that was, well, 20 years ago.
I tried to cruise the web to see if I could get up to date on what's happening, but web access to journals from here is pretty lame. So is the offering of ultrastructural type journals at our library, like, there aren't any.
I do get a few journals and I can access a few on the web, but most of the papers in the searchable index from JCB etc. that have SEM are, well, pretty old. Is there much modern research using SEM being done in biology these days? Mostly I see the SEM being applied to materials type research. Not that the biology work that has been done is not elegant, it just seems like a lot of it is finding its way on to journal covers or 'coffee table' picture books after being colorized in Photoshop.
I am OK with that, and if the kind of SEM that I know, simple fix, dehydr, and CPD is the state of the art, then I will feel better getting my students to do these things. But if there are sources for something new, I would like to be able to tell them about it. I mean, after all,how many fly eyes, bee's knees, and pollen grains can you look at?
Out on a limb, and with my asbestos suit fully zipped,
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n155A6nj005730 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07 -0600
==============================Original Headers============================== 22, 22 -- From lherault-at-bu.edu Thu Feb 5 08:42:34 2009 22, 22 -- Received: from relay10.bu.edu (relay10.bu.edu [128.197.27.62]) 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EgX5d032184 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:42:34 -0600 22, 22 -- X-Envelope-From: lherault-at-bu.edu 22, 22 -- Received: from BUSDM801203 ([155.41.214.83]) 22, 22 -- by relay10.bu.edu (8.13.1/8.13.1) with ESMTP id n15EgOoE002292 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 09:42:24 -0500 22, 22 -- From: "Ron L" {lherault-at-bu.edu} 22, 22 -- To: {microscopy-at-microscopy.com} 22, 22 -- References: {200902050512.n155Cjea010347-at-ns.microscopy.com} 22, 22 -- Subject: RE: [Microscopy] Current biological SEM 22, 22 -- Date: Thu, 5 Feb 2009 09:42:22 -0500 22, 22 -- Message-ID: {002401c9879f$f49d5030$53d6299b-at-ad.bu.edu} 22, 22 -- MIME-Version: 1.0 22, 22 -- Content-Type: text/plain; 22, 22 -- charset="us-ascii" 22, 22 -- Content-Transfer-Encoding: 7bit 22, 22 -- X-Mailer: Microsoft Office Outlook 11 22, 22 -- In-Reply-To: {200902050512.n155Cjea010347-at-ns.microscopy.com} 22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 22, 22 -- Thread-index: AcmHUGgTbUE/6JKoSnSCkEhcCgwrDgATlNQg ==============================End of - Headers==============================
I use 30 mM HEPES, 70 mM NaCl, 1 mM CaCl2, pH 7.4 which is roughly the same osmolarity as half-strength Karnovsky's. I have checked the pH after fixation and it seems stable. Tom
-----Original Message----- X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] Sent: Thursday, February 05, 2009 8:22 AM To: Phillips, Thomas E.
Listservers, What concentration of Hepes or Pipes would you use for buffering a standard TEM fixation (and subsequent washes), 0.1M, 50mM or 20 mM? Thanks for all responses.
Michael Delannoy
==============================Original Headers============================== 2, 27 -- From delannoy-at-jhmi.edu Thu Feb 5 08:19:50 2009 2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EJmkX017907 2, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:19:50 -0600 2, 27 -- X-IronPort-AV: E=Sophos;i="4.37,385,1231131600"; 2, 27 -- d="scan'208";a="186662157" 2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 05 Feb 2009 09:20:03 -0500 2, 27 -- Received: from johnshopkins.edu ([10.181.31.211]) by jesmail.johnshopkins.edu 2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 2, 27 -- with ESMTP id {0KEL00JV0JTEKC10-at-jesmail.johnshopkins.edu} for 2, 27 -- microscopy-at-microscopy.com; Thu, 05 Feb 2009 09:20:02 -0500 (EST) 2, 27 -- Received: from [10.181.192.191] (Forwarded-For: [10.16.66.59]) 2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Thu, 05 Feb 2009 09:20:02 -0500 2, 27 -- Date: Thu, 05 Feb 2009 09:20:02 -0500 2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 27 -- Subject: Hepes OR Pipes 2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 27 -- Message-id: {fc16d6674f2c.498aaf42-at-johnshopkins.edu} 2, 27 -- MIME-version: 1.0 2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 2, 27 -- Content-type: text/plain; charset=us-ascii 2, 27 -- Content-language: en 2, 27 -- Content-transfer-encoding: 7BIT 2, 27 -- Content-disposition: inline 2, 27 -- X-Accept-Language: en 2, 27 -- Priority: normal ==============================End of - Headers==============================
==============================Original Headers============================== 11, 29 -- From PhillipsT-at-missouri.edu Thu Feb 5 08:47:42 2009 11, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EldYo004448 11, 29 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:47:40 -0600 11, 29 -- X-IronPort-Anti-Spam-Filtered: true 11, 29 -- X-IronPort-Anti-Spam-Result: ApoEAO6KiknRauUp/2dsb2JhbADDP4UziEeEFgaCM4Mi 11, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 11, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Feb 2009 08:48:30 -0600 11, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 29 -- Thu, 5 Feb 2009 08:48:30 -0600 11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 29 -- Content-class: urn:content-classes:message 11, 29 -- MIME-Version: 1.0 11, 29 -- Content-Type: text/plain; 11, 29 -- charset="US-ASCII" 11, 29 -- Subject: RE: [Microscopy] Hepes OR Pipes 11, 29 -- Date: Thu, 5 Feb 2009 08:48:32 -0600 11, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05DF21EA-at-UM-XMAIL06.um.umsystem.edu} 11, 29 -- In-Reply-To: {200902051422.n15EMQqQ019086-at-ns.microscopy.com} 11, 29 -- X-MS-Has-Attach: 11, 29 -- X-MS-TNEF-Correlator: 11, 29 -- Thread-Topic: [Microscopy] Hepes OR Pipes 11, 29 -- Thread-Index: AcmHnTq5/3MiWONmQma9rYEPvbHj0AAA3WQA 11, 29 -- References: {200902051422.n15EMQqQ019086-at-ns.microscopy.com} 11, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 11, 29 -- To: {delannoy-at-jhmi.edu} , {microscopy-at-microscopy.com} 11, 29 -- X-OriginalArrivalTime: 05 Feb 2009 14:48:30.0235 (UTC) FILETIME=[CF7CCEB0:01C987A0] 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15EldYo004448 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gas19-at-chrysler.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gas19-at-chrysler.com Name: Gerald Shulke
Organization: Chrysler LLC
Title-Subject: [Microscopy] Puzzling EDX results
Question: Debby,
Was there any drift to the SEM image during the analysis? My assumption is that there was a slight drift in the signal over the 120 second collection time, and you moved to a slightly different chemistry in the sample. The solder probably does not have a very homogenous microstructure, so your image shifted to a different microstructural feature. Check to make sure your sample is adequately grounded and maybe select a larger area to collect the spectrum from, so there is more of an averaging of all of the microstructural constituents. Most likely there are small globules of lead, so you're drifting over these regions during the analysis. BSE should show the segregations.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] How to get rid of a magnetic field
Question: We want to move our EMís to a building close to tram tracks. At pre-installation measurements by FEI a distorting field of 10 mGauss (4.4 mGauss is the limit for a Tecnai12) was found. This building was a former MRI location; in the walls are steel (2cm thick) plating and a copper Faraday cage both with large holes in the ceiling. Can you give me advice on preventing this magnetic field? Is restoring the Faraday cage and/or the steel cage enough? Or do we need a mu-ferro or a Helmholtz cage? What will be the approximate costs of these?
==============================Original Headers============================== 9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G17MS011832 9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09 -0600 9, 13 -- Mime-Version: 1.0 9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]} 9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600 9, 13 -- To: microscopy-at-microscopy.com 9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver) 9, 13 -- Subject: viaWWW: How to get rid of a magnetic field 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 9, 13 -- Content-Transfer-Encoding: 8bit 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15G17MS011832 ==============================End of - Headers==============================
Hello, Does anyone have a manual for a Philips CM-200 FEG-TEM? We recently moved this TEM to our facility and it is now operational again, but the service rep says he cannot find a manual or any documentation and that the company just recently threw out all of the copies they had. If you have a manual could we get a copy? Sincerly, Valerie -- Valerie Lynch-Holm, Ph.D. Franceschi Microscopy & Imaging Center 133 Abelson Hall Washington State University Pullman, Wa 99164-4210 509-335-3025
==============================Original Headers============================== 1, 20 -- From vlynch-at-mail.wsu.edu Thu Feb 5 10:05:32 2009 1, 20 -- Received: from mx.wsu.edu.pph5a.pphosted.com (mx.wsu.edu.pph5a.pphosted.com [208.86.201.34]) 1, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G5VxN015328 1, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:05:32 -0600 1, 20 -- Received: from jaguar.it.wsu.edu (jaguar.it.wsu.edu [134.121.0.73]) 1, 20 -- by mx.wsu.edu.pph5a.pphosted.com (8.14.1/8.14.1) with ESMTP id n15G5gkN026596 1, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:05:43 -0800 1, 20 -- Received: from [134.121.42.104] (vrf.emc.wsu.edu [134.121.42.104]) 1, 20 -- by jaguar.it.wsu.edu (8.12.11.20060308/8.12.11) with ESMTP id n15G5glJ010027 1, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:05:42 -0800 1, 20 -- Mime-Version: 1.0 1, 20 -- Message-Id: {a06200700c5b0bd9eaa7e-at-[134.121.42.104]} 1, 20 -- Date: Thu, 5 Feb 2009 08:05:41 -0800 1, 20 -- To: Microscopy-at-microscopy.com 1, 20 -- From: Valerie Lynch-Holm {vlynch-at-mail.wsu.edu} 1, 20 -- Subject: Philips TEM CM-200 Manual? 1, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 20 -- X-Virus-Scanned: by amavisd-milter (http://amavis.org/) 1, 20 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-02-05_03:2009-02-05,2009-02-05,2009-02-05 signatures=0 1, 20 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0902050088 ==============================End of - Headers==============================
Jon, I agree. Biological SEM has changed and materials applications are more plentiful. However, in my opinion and experience, there is still room for the "old ways". Delta has a wonderful library of old SEM proceedings and there are many good papers in them. (Let's hope that "old" doesn't mean "obsolete" or we'd all be in trouble.). It does seems that environmental, low vacuum and high resolution SEM are most relevant nowadays, however, many biological labs don't have the luxury of owning them. As a technician who is responsible for SEM processing and training on a standard SEM, there is still a call for different biological preps. Your students would benefit from learning how to process cells (suspensions or grown on filters), OTO, cryo fracture, maceration, backscatter, replicas etc. Basic theory and hands on are crucial. When I was a student, we used the book "Preparation of Biological Specimens for Scanning Electron Microscopy" published by Scanning Electron Microscopy, Inc. and compiled by Judy Murphy and Godfried M. Roomans. It's from 1984 but it still has allot of ideas for your class. I'm not sure if it's still in print but there used to be one there at Delta. The more protocols they learn, the better equipped they will be when they graduate. Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {baskin-at-bio.umass.edu} To: {pekysar-at-ucdavis.edu} Sent: Thursday, February 05, 2009 12:15 AM
Jon,
The type of microscopy has to fit the question. SEM can be a very valuable biological tool if the question is appropriate. For instance, we do a lot of cryoSEM so that we do not have the artifacts (shrinkage, etc) that go with critical point drying.
I have one investigator who is interested in chemical crystal formation in plants. She does cryo and fractures the plant tissue to reveal the crystals in their native state without any possibility of extraction during aqueous fixation, etc.
Other uses revolve around food processing, biomedical products, and pharmaceutical applications with samples such as starch, collagen, and synthetic hydrogels. Documenting bacteria growth on food products before and after treatments to eliminate the bacteria would be meaningless if you had to process the sample as that would likely also remove bacteria. CryoSEM would preserve them on the sample.
Some samples can be done with traditional critical point drying even though there may be some distortion. An example would be to monitor cell growth on various substrates. Cells would be expected to shrink but their overall distribution would stay the same. Many plant tissues do well with CPD but others (very young plants) require cryoSEM.
And the list goes on for applications to biological (or hydrated samples in general...)
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: {jkrupp-at-deltacollege.edu} } Reply-To: {jkrupp-at-deltacollege.edu} } Date: Wed, 4 Feb 2009 23:12:05 -0600 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Current biological SEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } So, I thought I sent the following message to the list, but it never } showed up. I looked at again and wondered if I should resend it, at } first I wasn't going to do it, but then I decided, why not. After } reviewing it I thought it might be a little naive, but I am curious } about what you think. } } Here it is: } } Greetings } } At the risk of being labeled a heretic, I'd like to know the current } state of biological SEM. } } Here's the deal, I am teaching a class on bio SEM and just sat down to } prepare a lecture on modern biological SEM. I have lots of nice books } with cool SEM pictures, but I noticed they are all about 20 years old. } Heck, I even did some nice bio SEM, but that was, well, 20 years ago. } } I tried to cruise the web to see if I could get up to date on what's } happening, but web access to journals from here is pretty lame. So is } the offering of ultrastructural type journals at our library, like, } there aren't any. } } I do get a few journals and I can access a few on the web, but most of } the papers in the searchable index from JCB etc. that have SEM are, } well, pretty old. Is there much modern research using SEM being done } in biology these days? Mostly I see the SEM being applied to materials } type research. Not that the biology work that has been done is not } elegant, it just seems like a lot of it is finding its way on to } journal covers or 'coffee table' picture books after being colorized } in Photoshop. } } I am OK with that, and if the kind of SEM that I know, simple fix, } dehydr, and CPD is the state of the art, then I will feel better } getting my students to do these things. But if there are sources for } something new, I would like to be able to tell them about it. I mean, } after all,how many fly eyes, bee's knees, and pollen grains can you } look at? } } Out on a limb, and with my asbestos suit fully zipped, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original Headers============================== } 14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009 } 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu } [207.62.178.150]) } 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } n155A6nj005730 } 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07 -0600 } 14, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain } [127.0.0.1]) } 14, 42 -- by localhost (Email Security Appliance) with SMTP id } 221DF1BA6DD_98A6F4BB } 14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000 } (GMT) } 14, 42 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us } [207.62.178.236]) } 14, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id } 177F01BA6D5_98A6F4BF } 14, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 04:47:07 +0000 } (GMT) } 14, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) } 14, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) } 14, 42 -- with ESMTP id 45413886 for microscopy-at-microscopy.com; Wed, 04 Feb } 2009 21:10:01 -0800 } 14, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by } 14, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) } with } 14, 42 -- ESMTP id KEKTM900.61V for {microscopy-at-microscopy.com} ; } Wed, 4 } 14, 42 -- Feb 2009 20:54:09 -0800 } 14, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 648B68F74E48 } 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:01 -0800 } (PST) } 14, 42 -- X-Virus-Scanned: amavisd-new at } 14, 42 -- X-Spam-Flag: NO } 14, 42 -- X-Spam-Score: -2.499 } 14, 42 -- X-Spam-Level: } 14, 42 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 } tests=[AWL=0.000, } 14, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] } 14, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 14, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, } port 10024) } 14, 42 -- with ESMTP id VKwKzh5c2Zca for {microscopy-at-microscopy.com} ; } 14, 42 -- Wed, 4 Feb 2009 21:10:01 -0800 (PST) } 14, 42 -- Received: from [172.20.3.16] (unknown [172.20.3.16]) } 14, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 007508F74E1E } 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:10:00 -0800 } (PST) } 14, 42 -- Message-Id: {A7C0E8F7-7669-4A7A-A038-0F6A54694846-at-deltacollege.edu} } 14, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 14, 42 -- To: microscopy-at-microscopy.com } 14, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 14, 42 -- Content-Transfer-Encoding: 7bit } 14, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 14, 42 -- Subject: Current biological SEM } 14, 42 -- Date: Wed, 4 Feb 2009 21:10:00 -0800 } 14, 42 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 31 -- From dsherman-at-purdue.edu Thu Feb 5 10:30:49 2009 11, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15GUj2v008005 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:30:48 -0600 11, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 11, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n15GVLJB009976 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:31:21 -0500 11, 31 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 11, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n15GVLGp025477 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:31:21 -0500 11, 31 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 31 -- Thu, 5 Feb 2009 11:31:21 -0500 11, 31 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 11, 31 -- Thu, 5 Feb 2009 16:30:26 +0000 11, 31 -- User-Agent: Microsoft-Entourage/12.15.0.081119 11, 31 -- Date: Thu, 05 Feb 2009 11:30:20 -0500 11, 31 -- Subject: Re: [Microscopy] Current biological SEM 11, 31 -- From: Debby Sherman {dsherman-at-purdue.edu} 11, 31 -- To: {jkrupp-at-deltacollege.edu} , 11, 31 -- "message to: MSA list" {microscopy-at-microscopy.com} 11, 31 -- Message-ID: {C5B07E4C.3A89D%dsherman-at-exchange.purdue.edu} 11, 31 -- Thread-Topic: [Microscopy] Current biological SEM 11, 31 -- Thread-Index: AcmHrwkwaSMlGsvNS0+x3tSPJp4weg== 11, 31 -- In-Reply-To: {200902050512.n155C5DG008911-at-ns.microscopy.com} 11, 31 -- Mime-version: 1.0 11, 31 -- Content-type: text/plain; 11, 31 -- charset="US-ASCII" 11, 31 -- Content-transfer-encoding: 7bit 11, 31 -- X-OriginalArrivalTime: 05 Feb 2009 16:31:21.0084 (UTC) FILETIME=[2D99A3C0:01C987AF] 11, 31 -- X-PMX-Version: 5.4.0.320885 11, 31 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Hans, I can't answer your questions about the field and shielding, but it strikes me that you're also going to have a serious problem with vibration from the trams and it may trump the field problem.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl] Sent: Thursday, February 05, 2009 11:07 AM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] How to get rid of a magnetic field
Question: We want to move our EMís to a building close to tram tracks. At pre-installation measurements by FEI a distorting field of 10 mGauss (4.4 mGauss is the limit for a Tecnai12) was found. This building was a former MRI location; in the walls are steel (2cm thick) plating and a copper Faraday cage both with large holes in the ceiling. Can you give me advice on preventing this magnetic field? Is restoring the Faraday cage and/or the steel cage enough? Or do we need a mu-ferro or a Helmholtz cage? What will be the approximate costs of these?
==============================Original Headers============================== 9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G17MS011832 9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09 -0600 9, 13 -- Mime-Version: 1.0 9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]} 9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600 9, 13 -- To: microscopy-at-microscopy.com 9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver) 9, 13 -- Subject: viaWWW: How to get rid of a magnetic field 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 9, 13 -- Content-Transfer-Encoding: 8bit 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15G17MS011832 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From kenconverse-at-qualityimages.biz Thu Feb 5 10:35:30 2009 21, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15GZPeJ011759 21, 25 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:35:27 -0600 21, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com 21, 25 -- with ESMTP 21, 25 -- id {20090205163620.HKZI23506.cdptpa-omta04.mail.rr.com-at-Ken} ; 21, 25 -- Thu, 5 Feb 2009 16:36:20 +0000 21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 25 -- To: {j.janssen-at-nki.nl} , "MSA Listserver" {microscopy-at-microscopy.com} 21, 25 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field 21, 25 -- Date: Thu, 5 Feb 2009 10:36:14 -0500 21, 25 -- Message-ID: {9A8F23892CB340BA8B03AB7048159FEE-at-Ken} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="iso-8859-1" 21, 25 -- X-Priority: 3 (Normal) 21, 25 -- X-MSMail-Priority: Normal 21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 21, 25 -- Importance: Normal 21, 25 -- Thread-Index: AcmHq8m0BcyacC7XQ2iypTQc+cCrEAABL/Eg 21, 25 -- In-Reply-To: {200902051606.n15G6mV9017413-at-ns.microscopy.com} 21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15GZPeJ011759 ==============================End of - Headers==============================
In regards to the thread on Bio SEM and also the past thread from Frank Karl on trying to “see†Beryllium with EDS I would like to ask the community if the concept of the EDS optic to improve light element analysis would benefit applications in Bio SEM?
Dr Peter Ingram mentioned past use of EDS to find Be in human lung tissues, I believe that an application such as this would have benefited from the ability of acquiring up to 10 times the Be signal by the addition of this device.
I also believe that other applications exist and the optic qualifies as a new tool to increase the capabilities in many applications across the board including many in Bio SEM analysis.
We are in the midst of preparing a paper for the M&M conference and are looking for applications and samples that may benefit from this product. If you have any applications, samples, questions, or would like to talk about this please contact me offline.
---- lherault-at-bu.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our lab deals with Dental biomaterials and we have an SEM. The biological } part comes into play for tissue engineering, e.g. seeing if cells are in } scaffolds, are they happy with the surface they are on, etc. We've also } looked at bacteria in microleakage studies. Some of these problems are } unsolved but improved upon in 20 years. From what I've seen of research, } the more diverse the background you have, the more apt you are to come up } with a better solution to a problem. Remember Edison, the inventor of the } phonograph and practical light bulb? He had to come up with a complete } system to make the light bulb work outside of the lab. He studied how the } gas company distributed gas, an already tried and true system. And the } woman who invented the circular saw blade did so because she was using a } spinning wheel while the males of her family were pushing and pulling a saw } through wood. It's connections of old and new. } } Ron L } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: Thursday, February 05, 2009 12:13 AM } To: lherault-at-bu.edu } Subject: [Microscopy] Current biological SEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } So, I thought I sent the following message to the list, but it never } showed up. I looked at again and wondered if I should resend it, at } first I wasn't going to do it, but then I decided, why not. After } reviewing it I thought it might be a little naive, but I am curious } about what you think. } } Here it is: } } Greetings } } At the risk of being labeled a heretic, I'd like to know the current } state of biological SEM. } } Here's the deal, I am teaching a class on bio SEM and just sat down to } prepare a lecture on modern biological SEM. I have lots of nice books } with cool SEM pictures, but I noticed they are all about 20 years old. } Heck, I even did some nice bio SEM, but that was, well, 20 years ago. } } I tried to cruise the web to see if I could get up to date on what's } happening, but web access to journals from here is pretty lame. So is } the offering of ultrastructural type journals at our library, like, } there aren't any. } } I do get a few journals and I can access a few on the web, but most of } the papers in the searchable index from JCB etc. that have SEM are, } well, pretty old. Is there much modern research using SEM being done } in biology these days? Mostly I see the SEM being applied to materials } type research. Not that the biology work that has been done is not } elegant, it just seems like a lot of it is finding its way on to } journal covers or 'coffee table' picture books after being colorized } in Photoshop. } } I am OK with that, and if the kind of SEM that I know, simple fix, } dehydr, and CPD is the state of the art, then I will feel better } getting my students to do these things. But if there are sources for } something new, I would like to be able to tell them about it. I mean, } after all,how many fly eyes, bee's knees, and pollen grains can you } look at? } } Out on a limb, and with my asbestos suit fully zipped, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original Headers============================== } 14, 42 -- From jkrupp-at-deltacollege.edu Wed Feb 4 23:10:08 2009 } 14, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu } [207.62.178.150]) } 14, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } n155A6nj005730 } 14, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 23:10:07 } -0600 } } } ==============================Original Headers============================== } 22, 22 -- From lherault-at-bu.edu Thu Feb 5 08:42:34 2009 } 22, 22 -- Received: from relay10.bu.edu (relay10.bu.edu [128.197.27.62]) } 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15EgX5d032184 } 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 08:42:34 -0600 } 22, 22 -- X-Envelope-From: lherault-at-bu.edu } 22, 22 -- Received: from BUSDM801203 ([155.41.214.83]) } 22, 22 -- by relay10.bu.edu (8.13.1/8.13.1) with ESMTP id n15EgOoE002292 } 22, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 09:42:24 -0500 } 22, 22 -- From: "Ron L" {lherault-at-bu.edu} } 22, 22 -- To: {microscopy-at-microscopy.com} } 22, 22 -- References: {200902050512.n155Cjea010347-at-ns.microscopy.com} } 22, 22 -- Subject: RE: [Microscopy] Current biological SEM } 22, 22 -- Date: Thu, 5 Feb 2009 09:42:22 -0500 } 22, 22 -- Message-ID: {002401c9879f$f49d5030$53d6299b-at-ad.bu.edu} } 22, 22 -- MIME-Version: 1.0 } 22, 22 -- Content-Type: text/plain; } 22, 22 -- charset="us-ascii" } 22, 22 -- Content-Transfer-Encoding: 7bit } 22, 22 -- X-Mailer: Microsoft Office Outlook 11 } 22, 22 -- In-Reply-To: {200902050512.n155Cjea010347-at-ns.microscopy.com} } 22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 } 22, 22 -- Thread-index: AcmHUGgTbUE/6JKoSnSCkEhcCgwrDgATlNQg } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 21 -- From joexray-at-cinci.rr.com Thu Feb 5 10:37:41 2009 11, 21 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Gbdx6013092 11, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:37:40 -0600 11, 21 -- Received: from hrndva-web17-z02 ([10.128.132.168]) 11, 21 -- by hrndva-smta01.mail.rr.com with ESMTP 11, 21 -- id {20090205163836.RXHD4991.hrndva-smta01.mail.rr.com-at-hrndva-web17-z02} ; 11, 21 -- Thu, 5 Feb 2009 16:38:36 +0000 11, 21 -- Message-ID: {20090205163836.DOBTT.16831.root-at-hrndva-web17-z02} 11, 21 -- Date: Thu, 5 Feb 2009 11:38:36 -0500 11, 21 -- From: {joexray-at-cinci.rr.com} 11, 21 -- To: lherault-at-bu.edu 11, 21 -- Subject: Re: [Microscopy] RE: Current biological SEM 11, 21 -- Cc: Microscopy-at-microscopy.com 11, 21 -- In-Reply-To: {200902051458.n15Ewr8S022318-at-ns.microscopy.com} 11, 21 -- MIME-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=utf-8 11, 21 -- X-Priority: 3 (Normal) 11, 21 -- Sensitivity: Normal 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15Gbdx6013092 ==============================End of - Headers==============================
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Email: bob.price-at-uscmed.sc.edu Name: Bob Price
Organization: University of South Carolina School of Medicine
Question: The University of South Carolina School of Medicine will again be hosting a Basic Confocal Microscopy Workshop. This yearís workshop will be from June 15-19, 2009 and will include a series of lectures on the theory and applications of confocal microscopy, specimen preparation, processing confocal images in Photoshop, and 3D reconstructions using AMIRA. Students will be able to process triple labeled samples (cell cultures and sections) on site or bring their own samples to the workshop.
Faculty will include Drs. Jay Jerome (Vanderbilt), Ralph Albrecht (Univ Wisconsin-Madison), John Mackenzie (North Carolina State Univ), Tom TrusK (Medical Univ South Carolina) and myself. Instruments and applications experts from Leica, Nikon, Olympus, Perkin Elmer, Photometrics, and Zeiss are expected to be available for hands on training and imaging of samples.
Please register early as the last 4 workshops have been overbooked. Deadline for registration is June 1 as long as slots are available.
For further information and registration go to: http://dba.med.sc.edu/irf/price/irf/irf.htm or contact Anna McNeal (Anna.McNeal-at-uscmed.sc.edu)
Bob
Bob Price Research Professor Dept Cell Biol and Anat USC School of Medicine 6439 Garner's Ferry Road Columbia, SC 29208
Note: EDX map not attached to Listserver copy) Dear Debbie, There are a number of ways that this analysis may be compromised. I have attached a low magnification EDX map of the surface of standard solder. The lead is green and the tin is red. You can see how the phases segregate and the composition would vary greatly, depending on where and how large an area you analysed. The size of the various phases and grains will depend on the cooling rate of the solder. The other consideration is the state of the surface. EDX can only give reasonable analysis if the sample surface is flat and normal to the beam, so the x-ray take-off angle and solid angle are well-known and accurately entered into the EDX software. I always say that if you lie to the computer, he will give you the wrong answer. Everyone wants to get the numbers out, but they can be wildly inaccurate or imprecise if the geometry is wrong or unknown. If you are looking at a round wire, make sure that you are looking at the top, where it approximates a flat, normal-to-the-beam surface. In your case, I would take five to ten readings in different spots along the wire and average them, to get the overall solder content. I have the same problem when I look at leaded brass, where the lead is not alloyed with the brass, but remains in discreet little balls to act as a lubricant in machining. The analysis shows either no lead or 10% lead, when you hit a ball. Good luck. Explaining the pitfalls of the magic EDX machine is the hardest part of the job. Regards,
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: February 4, 2009 7:15 PM To: maryflet-at-interchange.ubc.ca
Hi all,
We were analyzing some samples of tin solder with varying but small amounts of lead. The solder was electroplated as a film (~7-10µm) on copper. We need to know % of lead in the samples with reasonable accuracy. The amounts of Pb varied from about 0.5 to 3% based on information from another quantifying technique.
We collected a spectrum for 120sec using 30kV (3000-5000cps) in an area and then repeated this on the same area two other times under identical conditions and immediately following the previous spectrum. Each time the ratio of Sn to Pb varied significantly. We are at a loss to explain why there was such a difference in the ratios from the identical area. Could this be due to something going on due to the repeated sampling of the same area?
Any idea as to why we had such poor reproducibility?
Thanks, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 12, 30 -- From dsherman-at-purdue.edu Wed Feb 4 21:07:58 2009 12, 30 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1537wD4026640 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 21:07:58 -0600 12, 30 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 12, 30 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n1537wpL022381 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 12, 30 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n1537woL011609 12, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 12, 30 -- Wed, 4 Feb 2009 22:07:58 -0500 12, 30 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 12, 30 -- Thu, 5 Feb 2009 03:07:50 +0000 12, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119 12, 30 -- Date: Wed, 04 Feb 2009 22:07:49 -0500 12, 30 -- Subject: Puzzling EDX results 12, 30 -- From: Debby Sherman {dsherman-at-purdue.edu} 12, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 12, 30 -- Message-ID: {C5AFC235.2672D%dsherman-at-purdue.edu} 12, 30 -- Thread-Topic: Puzzling EDX results 12, 30 -- Thread-Index: AcmHPuz1HKZ2V9XNDUeJmziVuCJ6lA== 12, 30 -- Mime-version: 1.0 12, 30 -- Content-type: text/plain; 12, 30 -- charset="ISO-8859-1" 12, 30 -- X-OriginalArrivalTime: 05 Feb 2009 03:07:58.0683 (UTC) FILETIME=[F2BAF2B0:01C9873E] 12, 30 -- X-PMX-Version: 5.4.0.320885 12, 30 -- X-PerlMx-Virus-Scanned: Yes 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1537wD4026640 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 31 -- From maryflet-at-interchange.ubc.ca Thu Feb 5 11:25:25 2009 23, 31 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5]) 23, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15HPNNF008069 23, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:25:24 -0600 23, 31 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 23, 31 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id 7332B1820E 23, 31 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 09:27:32 -0800 (PST) 23, 31 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 23, 31 -- by smtp.interchange.ubc.ca 23, 31 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 23, 31 -- with ESMTP id {0KEL00FYDSHV75-at-smtp.interchange.ubc.ca} for 23, 31 -- microscopy-at-microscopy.com; Thu, 05 Feb 2009 09:27:32 -0800 (PST) 23, 31 -- Date: Thu, 05 Feb 2009 09:27:21 -0800 23, 31 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 23, 31 -- Subject: FW: [Microscopy] Puzzling EDX results 23, 31 -- To: microscopy-at-microscopy.com 23, 31 -- Reply-to: maryflet-at-interchange.ubc.ca 23, 31 -- Message-id: {0KEL00FYESHV75-at-smtp.interchange.ubc.ca} 23, 31 -- Organization: Materials Eng. 23, 31 -- MIME-version: 1.0 23, 31 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 23, 31 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 23, 31 -- Content-type: text/plain; charset=iso-8859-1 23, 31 -- Thread-index: AcmHP+h99ywAcSieSmaj/M7083feXwAdA7tAAAC6EoA= 23, 31 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.2.5.171326 23, 31 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 23, 31 -- X-PerlMx-Spam: Probability=8%, Report=__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0 23, 31 -- X-Spam-Level: 23, 31 -- X-Spam-Flag: No 23, 31 -- Content-Transfer-Encoding: 8bit 23, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15HPNNF008069 ==============================End of - Headers==============================
I need to clarify my request yesterday regarding hiring reps and distributors, based partly on the response I received.
Parallax is looking to hire INDEPENDENT manufacturer's reps and distributors for our new x-ray microanalysis products (LoMAX EDS optic, WDS, EDS, x-ray components). If you know such a person, or company, in your area (or others) that you are familiar with and like, I would greatly appreciate a recommendation. Please give them my contact info, or provide me their contact info. We are interested in talking to anyone who is interested.
Thank you again for your time and attention.
Regards,
Don
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: dkloos-at-parallaxray.com [mailto:dkloos-at-parallaxray.com] Sent: Wednesday, February 04, 2009 12:32 PM To: dkloos-at-parallaxray.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dkloos-at-parallaxray.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dkloos-at-parallaxray.com Name: Don Kloos
Organization: Parallax Research, Inc.
Title-Subject: [Filtered] Parallax Research is looking for reps to hire
Question: Hello All:
I need your help. Parallax Research is seeking to hire manufacturer's reps and distributors to handle its new EDS and WDS x-ray microanalysis products in North America, Europe, Asia, and other regions of the world. If you know of an individual or company in your area that you like to deal with and can recommend them, please let me know.
Thank you for your time.
Don Kloos Parallax Research. Email: dkloos-at-parallaxray.com. Phone: 1 866 581-XRAY, or +1 714 897-9779. www.parallaxray.com
Situation with EMI strongly depends on what kind of field are you dealing with, what is the frequency of magnetic interference. DC field, low-frequency (like 50Hz from power line) and high frequency (RF and HF portion of spurious fields from sparks) all behave differently. To get better idea about the nature of the problem you need to do EMI survey and obtain a plot of field vs. frequency. If this was already done then posting a link to the plot of the results could be helpful.
Without going too deep into the details, restoring faraday cage will help with RF, but would do nothing for 50Hz or DC field. Because of substantial thickness of the steel walls (2cm) restoring continuity of magnetic cage will measurably weaken your 50Hz and higher frequency magnetic noise. By the way - is the floor also shielded by the same 2cm steel? If your field is DC then it may actually be coming from magnetized steel cage itself (Curious: is it really steel and not a magnetically soft iron?) In this case you can try to de-magnetize it.
About four years ago I had great experience with magnetic field cancellation system from http://www.spicerconsulting.com/ - talk to them about your particular case and try to see if they can arrange a demo setup at your site to see how much improvement would you get in your specific case. No interest here, just a (former) but very satisfied customer.
Keep in mind that magnetic cancellation systems have certain response time. They great in canceling steady-state or slow-changing AC fields, but it takes some time to adjust cancellation in response to rapid change of the interference. Therefore when and if you get occasional strong spark from the tram or arc, welder some of it will get through the cancellation and may potentially ruin that particular image you were taking.
Hope this helps, if you have more questions please feel free to contact on or off-list.
Cheers, Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl] Sent: Thursday, February 05, 2009 11:03 AM To: vray-at-partbeamsystech.com
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Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] How to get rid of a magnetic field
Question: We want to move our EMís to a building close to tram tracks. At pre-installation measurements by FEI a distorting field of 10 mGauss (4.4 mGauss is the limit for a Tecnai12) was found. This building was a former MRI location; in the walls are steel (2cm thick) plating and a copper Faraday cage both with large holes in the ceiling. Can you give me advice on preventing this magnetic field? Is restoring the Faraday cage and/or the steel cage enough? Or do we need a mu-ferro or a Helmholtz cage? What will be the approximate costs of these?
==============================Original Headers============================== 9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G17MS011832 9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09 -0600 9, 13 -- Mime-Version: 1.0 9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]} 9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600 9, 13 -- To: microscopy-at-microscopy.com 9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver) 9, 13 -- Subject: viaWWW: How to get rid of a magnetic field 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 9, 13 -- Content-Transfer-Encoding: 8bit 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15G17MS011832 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 26 -- From vray-at-partbeamsystech.com Thu Feb 5 11:42:50 2009 24, 26 -- Received: from smtp100.biz.mail.re2.yahoo.com (smtp100.biz.mail.re2.yahoo.com [206.190.52.46]) 24, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n15Hgmjd024807 24, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 11:42:49 -0600 24, 26 -- Message-Id: {200902051742.n15Hgmjd024807-at-ns.microscopy.com} 24, 26 -- Received: (qmail 74115 invoked from network); 5 Feb 2009 17:45:29 -0000 24, 26 -- Received: from unknown (HELO cp1198275a) (vray-at-75.67.14.43 with login) 24, 26 -- by smtp100.biz.mail.re2.yahoo.com with SMTP; 5 Feb 2009 17:45:29 -0000 24, 26 -- X-YMail-OSG: 322IYPAVM1nxyVFrJLWbhPO4NGejQsrDZyW24Wx_SNz0fFzkvshuxXy6chafZLvbiACdkNH9fJhdnE0wcPH0z4Jmh6hEc2e5b9cWuPGLFW7nZ4I_GImp3hM3qZefrkTWCSu6LHVYf_J43dLdrDUixVz9x8IhZjQwhOEbI56KoH2ZChoF9TjKGPSVUyOvBmR_GB2v3A4nqZ8oQykTpp3BnXXicb7TnCZE 24, 26 -- X-Yahoo-Newman-Property: ymail-3 24, 26 -- Reply-To: {vray-at-partbeamsystech.com} 24, 26 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 24, 26 -- To: {j.janssen-at-nki.nl} 24, 26 -- Cc: {microscopy-at-microscopy.com} 24, 26 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field 24, 26 -- Date: Thu, 5 Feb 2009 12:48:22 -0500 24, 26 -- Organization: PBST / MEO Engineering 24, 26 -- MIME-Version: 1.0 24, 26 -- Content-Type: text/plain; 24, 26 -- charset="iso-8859-1" 24, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 24, 26 -- Thread-Index: AcmHqz47KwN3pd6URkidOnTRaHrKGAACSVQg 24, 26 -- In-Reply-To: {200902051602.n15G2vR4013419-at-ns.microscopy.com} 24, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 24, 26 -- Content-Transfer-Encoding: 8bit 24, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15Hgmjd024807 ==============================End of - Headers==============================
Regarding the 'biosem' and 'envirosem' topic, can somebody explain how light element analysis is (or isn't) done with EDS and WDS in these circumstances?
Can light elements be analyzed in these environments and is this even done?
Thanks,
Don
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Thursday, February 05, 2009 12:15 AM To: dkloos-at-parallaxray.com
Jonathan, You ask a relevant question. As a biologist who has gotten deeply into SEM in the last decade, I am surprised that there is not more use on our side of the fence. I suspect that most of the recent advances in SEM tech have been driven my materials folks and that the biologists simply don't know about them. Let me give you my perspective on where I think the advances are.
1. Environmental SEM. It is now possible to examine a fully hydrated specimen in the SEM. The sample needs to be frozen or at least cold to minimize evaporation but otherwise not processed at all. I am aware of people who use ESEM to study root hairs and also floral meristems. I suspect animal scientists use also. The point is that fixation crtical point dry and coating are all avoided and instead the sample is directly viewed. The drawback is that once the sample is out of the machine it cannot be viewed again later. And the resolution of an ESEM is no better than a conventional tungsten filament model.
2. Wet chambers. A rather recent (5 years or so?) development are thin membranes that the beam can pass but gas cannot. This allows fully hydrated samples to be viewed at ambient temp and pressure in chambers built with the top surface being that special membrane. Thus living cells can be grown on the membrane (inside the membrane) and viewed in the SEM while they are alive. It is of course a question how much radiation damage the beam will induce. Still the cells are certanly viable for a while. I have seen papers using this tech on animal tissue culture cells. There was quite a lot of excitement when these chambers hit the market but I don't know how successful they have proven in the "real" world.
The above points are geared for eliminating fixation and looking at samples as close to living as possible. This is clearly difficult given the fundamental high-vacuum nature of the SEM beast. The other direction where advances lay is in the high vac, high resolution end of things. Here the advances in SEM design have been phenomenal. The field emission gun allows resolution to be obtained that is almost as good as the best TEMs and certainly far beyond what the good old fly eye SEM was capable of seeing.
3. High resolution. With the field emission gun, macromolecules can be resolved. I among others have taken advantage of this to study the ultrastructure of the plant cell wall. Cellulose microfibrils are on order of 10 nm and these can be readily imaged with FESEM. What is particularly useful for me is that I can put my plant stem 1 mm by 10 mm in to instrument, get good low mag survey views and crank up the magnficiation to the ultrastructual level in any cell on the sample that is appropriate. The easy moving between low and high magnifications is extremely helpful where extensive sampling is needed or when rare features need to be found. Other biological systems that have been profitably studied with FESEM include the cytoskeleton, bacterial biofilms, biomineralization, and nuclear pore complexes. All of these cases involve complex three dimensional structures and the FESEM lets you image them at high resolution in the intact (or semi-intact) state.
4. Low voltage. Along with the sharper probe of the FESEM comes the ability to work at really low accelerating voltage. In the recent vintage FESEM's it is easy to image at 1 kV and possible to go down even as low as a tenth of that. This has several advantages. First, many samples are destroyed or damaged at 30 kV or even at 10 kV. Second, the lower the beam voltage the more exclusively the image comes from the surface. This increases the resolution in the depth direction.
Both low voltage and the smaller probe size allow much thinner metal coats to be applied, and in some cases no coat at all. For the work I have been doing on the cell wall, I use ca. 1 to 2 nm of Pt. This is an order of magnitude less than typical gold coats for conventional tungsten SEM work.
5. Backscattered imaging. Of particular importance to biologists is being able to image backscattered electrons. This allows the localization of gold probes, as in gold conjugated secondary antibodies. With the latest instrumentation, one can colect electrons from selected energy (or angle) levels and mix signals from several detectors. Thus, the SE detector can show the topography of the sample while the BSE detector can pick up the signal from the gold and the two are overlain seamlessly. The sensitivity of the detectors is such that the gold can be imaged at high contrast even with a sample lightly covered with platinum. I took advantage of this (with slightly older not quite so performant technology) to detect gamma tubulin in the plant cell cortex. Others have localized antigens on the plasma membrane and cell wall, to name a few.
There have been parallel developments in elemental analysis (EDS and related modes) and I expect biologists have taken advantage of these too but it is not my area so I don't know. Perhaps a helpful netizen will chime in.
Summarizing, you are right, recent use of SEM in biology is a little bit burried. However these uses will be well worth your digging up. Good luck with your course, Tobias
} } } Greetings } } At the risk of being labeled a heretic, I'd like to know the current } state of biological SEM. } } Here's the deal, I am teaching a class on bio SEM and just sat down to } prepare a lecture on modern biological SEM. I have lots of nice books } with cool SEM pictures, but I noticed they are all about 20 years old. } Heck, I even did some nice bio SEM, but that was, well, 20 years ago. } } I tried to cruise the web to see if I could get up to date on what's } happening, but web access to journals from here is pretty lame. So is } the offering of ultrastructural type journals at our library, like, } there aren't any. } } I do get a few journals and I can access a few on the web, but most of } the papers in the searchable index from JCB etc. that have SEM are, } well, pretty old. Is there much modern research using SEM being done } in biology these days? Mostly I see the SEM being applied to materials } type research. Not that the biology work that has been done is not } elegant, it just seems like a lot of it is finding its way on to } journal covers or 'coffee table' picture books after being colorized } in Photoshop. } } I am OK with that, and if the kind of SEM that I know, simple fix, } dehydr, and CPD is the state of the art, then I will feel better } getting my students to do these things. But if there are sources for } something new, I would like to be able to tell them about it. I mean, } after all,how many fly eyes, bee's knees, and pollen grains can you } look at? } } Out on a limb, and with my asbestos suit fully zipped, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } }
Hello Jerzy, Thank you for your response. We are in contact with our local FEI service group they are the ones who told us that Philips recently destroyed all it's manual copies for the Philips CM200. The service engineer we are working with says he does not have acess to or cannot find any form of publication about our scope. We are trying to write operating protocols for general use, x-ray diffraction and EDAX and we are trying to learn how to operate it so we can teach/help our students, faculty and clients. If you have any step by step instruction for x-ray diffraction and EDAX that would be very helpful. Valerie
} Valerie, } } There are multiple large-volumes (300page and up) manuals for the } CM-series instruments: Mechanical, Electronics, and couple of shorter } publications from Philips electron Optics on operations. I suggest you } contact your local FEI service group; they have CDs with those manuals } in PDF format issued to service engineers, perhaps you can get a copy of } them. I have Cm300FEG and many of the features between these instruments } are similar, if you need specific stuff I might be able to scan few } pages and e-mail them to you. } } I think the FEI call number for service is 1-888-ION-MILL. } } Jerzy } *************************************************** } Jerzy Gazda Ph.D. } Section Manager - TEM, FIB, SEM } Cerium Laboratories LLC } 5204 E. Ben White Blvd. MS-512 } Austin, TX 78741 } } (512) 934-5185 vm } (512) 622-6600 pgr } } www.ceriumlabs.com } } -----Original Message----- } From: vlynch-at-mail.wsu.edu [mailto:vlynch-at-mail.wsu.edu] } Sent: Thursday, February 05, 2009 10:19 AM } To: Gazda, Jerzy } Subject: [Microscopy] Philips TEM CM-200 Manual? } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America
==============================Original Headers============================== 3, 24 -- From vlynch-at-mail.wsu.edu Thu Feb 5 15:11:03 2009 3, 24 -- Received: from mx.wsu.edu.pph5a.pphosted.com (mx.wsu.edu.pph5a.pphosted.com [208.86.201.34]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15LB0ih006200 3, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 15:11:02 -0600 3, 24 -- Received: from jaguar.it.wsu.edu (jaguar.it.wsu.edu [134.121.0.73]) 3, 24 -- by mx.wsu.edu.pph5a.pphosted.com (8.14.1/8.14.1) with ESMTP id n15LAt26003077 3, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 13:10:56 -0800 3, 24 -- Received: from [134.121.42.104] (vrf.emc.wsu.edu [134.121.42.104]) 3, 24 -- by jaguar.it.wsu.edu (8.12.11.20060308/8.12.11) with ESMTP id n15LAtCY011566 3, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 13:10:55 -0800 3, 24 -- Mime-Version: 1.0 3, 24 -- Message-Id: {a06200708c5b104172f0d-at-[134.121.42.104]} 3, 24 -- In-Reply-To: 3, 24 -- {B8013C1F41F45F4886A4F71F775DF1587383BF-at-USAUSEXMBPF1.spansion.com} 3, 24 -- References: {200902051618.n15GIswj003085-at-ns.microscopy.com} 3, 24 -- {B8013C1F41F45F4886A4F71F775DF1587383BF-at-USAUSEXMBPF1.spansion.com} 3, 24 -- Date: Thu, 5 Feb 2009 13:10:53 -0800 3, 24 -- To: Microscopy-at-microscopy.com 3, 24 -- From: Valerie Lynch-Holm {vlynch-at-mail.wsu.edu} 3, 24 -- Subject: RE: [Microscopy] Philips TEM CM-200 Manual? 3, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 24 -- X-Virus-Scanned: by amavisd-milter (http://amavis.org/) 3, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-02-05_03:2009-02-05,2009-02-05,2009-02-05 signatures=0 3, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0811170000 definitions=main-0902050126 ==============================End of - Headers==============================
I prefer the Epson Perfection V750-M Pro Scanner. It costs around $700, but the extra money is worth it for the extra software and calibration standard that is included. It makes a pretty decent stand-in for a stereoscopic microscope, too.
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
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} I like to scan plate film (Philips EM300) , can you please suggest whic
} one is suitable for scaaning the EM films. } } Thank you very much for your help. } } V.Kabilan } UMDNJ-NJDS } Newark, USA
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==============================Original Headers============================== 11, 34 -- From Robert.Zonis-at-Sanford.com Thu Feb 5 16:09:50 2009 11, 34 -- Received: from mail142.messagelabs.com (mail142.messagelabs.com [216.82.249.99]) 11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15M9nuW004441 11, 34 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:09:50 -0600 11, 34 -- X-VirusChecked: Checked 11, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 11, 34 -- X-Msg-Ref: server-8.tower-142.messagelabs.com!1233871787!65085875!1 11, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 11, 34 -- X-Originating-IP: [198.176.16.25] 11, 34 -- Received: (qmail 12669 invoked from network); 5 Feb 2009 22:09:47 -0000 11, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25) 11, 34 -- by server-8.tower-142.messagelabs.com with RC4-SHA encrypted SMTP; 5 Feb 2009 22:09:47 -0000 11, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 11, 34 -- Thu, 5 Feb 2009 16:09:46 -0600 11, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 34 -- Content-class: urn:content-classes:message 11, 34 -- MIME-Version: 1.0 11, 34 -- Content-Type: text/plain; 11, 34 -- charset="us-ascii" 11, 34 -- Subject: RE: [Microscopy] Scanner for Plate film 11, 34 -- Date: Thu, 5 Feb 2009 16:07:49 -0600 11, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401950A46-at-naoaksasebe02.nr.ad.newellco.com} 11, 34 -- In-Reply-To: {200902052159.n15LxEks031584-at-ns.microscopy.com} 11, 34 -- X-MS-Has-Attach: 11, 34 -- X-MS-TNEF-Correlator: 11, 34 -- Thread-Topic: [Microscopy] Scanner for Plate film 11, 34 -- Thread-Index: AcmH3P7d8bcKCLdLQA+8rheHYN/2EQAAGMCA 11, 34 -- References: {200902052159.n15LxEks031584-at-ns.microscopy.com} 11, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 11, 34 -- To: {velliyka-at-umdnj.edu} 11, 34 -- Cc: {microscopy-at-microscopy.com} 11, 34 -- X-OriginalArrivalTime: 05 Feb 2009 22:09:46.0856 (UTC) FILETIME=[74C88E80:01C987DE] 11, 34 -- Content-Transfer-Encoding: 8bit 11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15M9nuW004441 ==============================End of - Headers==============================
Dear All, I am new here and this is my first response after reading Netnotes in Microscopy Today for years. Thanks for all the great information. Please pardon any initial unintended errors in protocol. I am an old dog learning new tricks so I would like members of this discussion to consider reconciling a couple of concepts. 1. Materials and Methods – complete reporting. 2. Significant figures (mathematically speaking). I was trained for EM and photography back in the days of glass plates and drum dryers with Pacasol. I now work in an age where we have discarded all our film photography materials and work with digital cameras and software. In the old days, dogging and burning were standard techniques in the dark room to get your EM picture to look perfect. No white spots from dust were allowed, and bits of stain in the lumen of an open perfused blood vessel were eliminated with a conveniently placed label and arrow. That was never considered as altering the significance of the data which the image conveyed and that was by an instructor who always stressed complete descriptions in the materials and methods section of everything that was done, all equipment used, etc. No one ever reported that the left corner of Fig.1 was dogged for 3 seconds while printing on grade 3 paper with the developer at 22 degrees centigrade. I have done enough EM to know that sometimes the picture you end up with has aesthetic defects. I am equally sure that most in not all the published EM (or LM, SEM, etc) images have that conveniently placed arrow or label somewhere. Should that have been reported then and or now? How is it any different if you remove what you know to be dirt or a stain particle from an image by any digital subtraction or addition or the rubber stamps method then by placing that convenient label or arrow over it and then merging the layers? You still have changed pixel values. I personally do not see the difference nor do I think it necessary to state in the figure legend or an appendix all the aesthetic alterations to an image. Also, I can not image a journal allowing you to. Even with online publishing I am constantly being told by the journals to make things shorter.
As for the data contained in the digital image, the amount contained in individual pixel values and pixels per image goes way beyond the number of significant figures relevant to the data being presented by the image. And before you even get to Photoshop, the camera software lets you alter the image intensity, number of bits in an image (8, 12, 16) etc. To me it makes perfect sense to have a dark and light background image (as previously mentioned) and apply them to the image in question to eliminate defects in the optical path and report that in the M&M. However, if the point of the picture in question depends on the number of an individual pixel or the numbers of pixels in an image, then it makes sense to include any alterations in the M&M section. However, I suspect that for a very large percentage of the images published, a rubber stamped alteration of a capillary lumen for aesthetic purposes would be immaterial even though it changed the original values of the pixels in question. I would never consider that scientific fraud. Even when the pixels in question might have significance, when does the issue of reporting significant figures come in to the issue? I can accept that there are images produced where the data from the image are the pixel values. But, as I am mostly biologically oriented that does not happen very often to me. There are lots of papers out there in the early days of immunohistochemistry which tried to equate stain intensity (the number of a given pixel) with amount of pathology (numbers of pixels equal to or greater than a given pixel value per image with the right RGB or CMY or HIS, etc values) only to discover that the errors derived from trying to mix those two parameters yielded only false results. As my EM instructor always pointed out, everything we report is an “artifact”. So, if pixel values are that important, when do we apply the test of significant figures to the pixel data. If I publish 1 picture from the skin of 1 test animal showing “representative” damage to the basal lamina in an experimental group (lets say 15 control and 15 experimental animals) then anyone who accepts my picture might further question the M&M section, for number of images taken per section, per sample, per animal and further ask if the data were derived from a sterologic process of sampling or whether I just got lucky. I rarely see any of this data reported. I realize as was said earlier that the day is coming when there will be enough space on the journal’s server for them to require that the original unaltered image be placed there along with the published one (in which you placed that convenient arrow) but then how do we know that an unaltered image was placed there in the first place? We do not -- we trust in the integrity within our community, and although fraud does occur is it at such a rate that it is really an issue? I suppose the well trained individual could download the original of a “questionable” published image and subject it to an analysis which would show pixel alterations and that would be good if one suspected deliberate fraud. My guess is that it would be a very rare thing. To close, I am a firm believer in complete M&M reporting and that would include how I took my image – camera, microscope, software, and techniques used, but I can not see burdening readers with the morally appropriate mea culpa that I typed a figure label or placed an arrow over an aesthetic defect and merged the layers to make a picture more aesthetically pleasing, or that I “rubberstamped” a piece of dirt. Thanks for considering these issues. Steve
Steven P. Tinling Ph.D. Director of Research Otolaryngology Research Lab University of California, Davis 1515 Newton Ct., Rm. 209 Davis, CA. 95616-4859 Phone: 530-754-5045 Fax: 530-754-5046
==============================Original Headers============================== 5, 22 -- From sptinling-at-ucdavis.edu Thu Feb 5 16:14:10 2009 5, 22 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15ME9O8011215 5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb 2009 16:14:10 -0600 5, 22 -- Received: from MOTO-2951.ucdavis.edu ([128.120.223.66]) 5, 22 -- (authenticated bits=0) 5, 22 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n15ME3sK026356 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb 2009 14:14:04 -0800 (PST) 5, 22 -- Message-Id: {200902052214.n15ME3sK026356-at-warsaw.ucdavis.edu} 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 5, 22 -- Date: Thu, 05 Feb 2009 14:14:28 -0800 5, 22 -- To: Microscopy-at-Microscopy.Com 5, 22 -- From: Steve Tinling {sptinling-at-ucdavis.edu} 5, 22 -- Subject: Image Processing -- Ethics and Validity 5, 22 -- Mime-Version: 1.0 5, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 5, 22 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av5 5, 22 -- X-Virus-Status: Clean 5, 22 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15ME9O8011215 ==============================End of - Headers==============================
The perspective from an institution focused almost entirely on human medical and related biological research studies puts SEM in the far background. Whereas, there was once several SEM instruments on campus there is now only one old, mostly inaccesible instrument in the Dental School. I might hear an inquiry about SEM resources once a year and I have to direct those investigators to a lab at another institution that enjoys a full spectrum of departments and science fields. The vast majority of funding and interest primarily focuses on genetic and molecular questions. The SEM does not provide much useful data for these studies though it is invaluable for descriptive illustrations. I think this is supported by the dearth of SEM data in mamalian biology studies in high profile publications such as Science, Nature and Cell. In contrast light microscopy, particularly laser scanning confocal microscopy, is booming. I estimate that there are over 30 heavily used confocal systems at this university and light micrographs are commonly found in research publications. So as much as I pine for the good old days the reality that I experience is that SEM is a minor methodology in current biomedical research. -- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 4, 39 -- From Larry.Ackerman-at-ucsf.edu Thu Feb 5 16:21:43 2009 4, 39 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 4, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15MLgMi029033 4, 39 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:21:43 -0600 4, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with 4, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2)); 4, 39 -- Thu, 05 Feb 2009 14:21:31 -0800 4, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B 4, 39 -- X-AuditID: 403623d2-abe1ebb000007fb9-fb-498b82a9d657 4, 39 -- Received: from exbhmcb02.ucsfmedicalcenter.org ( 4, 39 -- exbhmcb02.ucsfmedicalcenter.org [64.54.46.223]) by 4, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id 4, 39 -- B18DC1426 for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:22:01 4, 39 -- -0800 (PST) 4, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by 4, 39 -- exbhmcb02.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830); 4, 39 -- Thu, 5 Feb 2009 14:21:31 -0800 4, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 4, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 5 Feb 4, 39 -- 2009 14:21:31 -0800 4, 39 -- Message-ID: {498B666B.3080508-at-ucsf.edu} 4, 39 -- Date: Thu, 05 Feb 2009 14:21:31 -0800 4, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 4, 39 -- Reply-to: larry.ackerman-at-ucsf.edu 4, 39 -- Organization: UCSF, NeuroAnatomy 4, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 4, 39 -- MIME-Version: 1.0 4, 39 -- To: Microscopy-at-microscopy.com 4, 39 -- Subject: Re: [Microscopy] Current biological SEM 4, 39 -- References: {200902051657.n15GvPdW014913-at-ns.microscopy.com} 4, 39 -- In-Reply-To: {200902051657.n15GvPdW014913-at-ns.microscopy.com} 4, 39 -- X-OriginalArrivalTime: 05 Feb 2009 22:21:31.0459 (UTC) 4, 39 -- FILETIME=[18C27130:01C987E0] 4, 39 -- X-Brightmail-Tracker: AAAAAQNEgKM= 4, 39 -- X-WSS-ID: 6595B9E11SS1928881-01-01 4, 39 -- Content-Type: text/plain; 4, 39 -- charset=iso-8859-1; 4, 39 -- format=flowed 4, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a concern regarding the separation of the iodoform and epoxy prior to and following curing. Iodoform, being a small molecule, conceivable could diffuse out of the epoxy resin into small voids faster than the curing epoxy resin. If variable diffusion of iodoform and epoxy did occur, might it not lead to poor infiltration of the sample? In your work, have you seen any indication or are you aware of any instance in which iodoform phase separates from the epoxy resin?
Thanks,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
wesaia-at-iastate .edu To gary.m.brown-at-exxonmobil.com 01/28/09 02:27 cc PM Subject [Microscopy] RE: Pre-embedding Please respond staining of embedding media to wesaia-at-iastate .edu
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Years ago (1980s), I followed someone else's lead to dope epoxy resin with iodoform to increase its average atomic number.
I was studying minerals in coal and standard epoxy resins provided practically no contrast with coal in backscattered electron images. We ended up dissolving about 15 wt.% iodoform in epoxy resin. We later added the hardener and the epoxy behaved in much the same way as the original two-part epoxy. That is, hardness and polymerization were similar.
The resulting epoxy offered significant contrast with coal and allowed us to proceed with automated image analyses.
Be advised that iodoform is rather nasty and needs to be handled with care.
For what it's worth, I began working with iodoform shortly after the Right-to-know laws were passed. Suppliers had recently started including MSDSs with all their chemicals. I was still bemused that my chemical-grade calcium carbonate "should be disposed of in an approved, chemical-waste landfill" when my iodoform arrived. I had to do some more of my own research to determine if iodoform was really as nasty as the MSDS said or not. (It is.) Someone was crying wolf about the calcium carbonate while a contractor was laying tons of the stuff just outside our building as a base for the parking lot.
Warren S.
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Wednesday, January 28, 2009 1:03 PM To: wesaia-at-iastate.edu
Hi Listers, } } I have a lot of well aged photographic paper. I don't } know how old paper can be and still work, but I have one } vintage box from 1975 and several from the 80's } } If you miss the disco era and think you could use this } paper let me know. } } My lab is being closed down for repairs starting Tuesday. } So it you act fast and pay to have it shipped I can send it } you on Monday. Otherwise you have to wait until the A/C } repairs are completed (84 degree confocal rooms anyone) some } time in March.
Paula
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 } }
==============================Original Headers============================== 7, 27 -- From vapatpxs-at-yahoo.com Thu Feb 5 16:46:57 2009 7, 27 -- Received: from n77.bullet.mail.sp1.yahoo.com (n77.bullet.mail.sp1.yahoo.com [98.136.44.45]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n15MktgF028489 7, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:46:56 -0600 7, 27 -- Received: from [69.147.84.145] by n77.bullet.mail.sp1.yahoo.com with NNFMP; 05 Feb 2009 22:46:53 -0000 7, 27 -- Received: from [69.147.84.116] by t8.bullet.mail.sp1.yahoo.com with NNFMP; 05 Feb 2009 22:46:53 -0000 7, 27 -- Received: from [127.0.0.1] by omp208.mail.sp1.yahoo.com with NNFMP; 05 Feb 2009 22:46:53 -0000 7, 27 -- X-Yahoo-Newman-Property: ymail-3 7, 27 -- X-Yahoo-Newman-Id: 697000.91640.bm-at-omp208.mail.sp1.yahoo.com 7, 27 -- Received: (qmail 40266 invoked by uid 60001); 5 Feb 2009 22:46:53 -0000 7, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 27 -- s=s1024; d=yahoo.com; 7, 27 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 27 -- b=AZy5v26uwobEylQ/9SdM2Pit5pwdjQ/7PMpvQMoyKnRhPDMi05YUniHKstAiHy3UlzpQteQgUSrNGlRK9BEjSQEdai+lvG8acfLl4upUK8xK7VerCKzaP+zffMiT/vh0+OItFcIafvh+2cJNjiGRZLkSdlCjqDEDf65kMZzdii8=; 7, 27 -- X-YMail-OSG: xrWb3hQVM1kYUJj5Q.7IrtF9aUt3d7WYuSGPcUUcc6saJnf5piUHk5buHwDJPRS8Cl7Abzd87ITXkpJ.nStPftObYsbyReeJ2MbzJGBA9f9H2Btlr3p0IgWa3gmTziX0KSdfocWljHuL3sgsO1B_ESyjTD8- 7, 27 -- Received: from [152.132.10.194] by web46102.mail.sp1.yahoo.com via HTTP; Thu, 05 Feb 2009 14:46:53 PST 7, 27 -- X-Mailer: YahooMailWebService/0.7.260.1 7, 27 -- Date: Thu, 5 Feb 2009 14:46:53 -0800 (PST) 7, 27 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 7, 27 -- Reply-To: vapatpxs-at-yahoo.com 7, 27 -- Subject: Kodak, Ilford and Agfa Paper 7, 27 -- To: MSA BB {Microscopy-at-microscopy.com} 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; charset=iso-8859-1 7, 27 -- Message-ID: {369567.39596.qm-at-web46102.mail.sp1.yahoo.com} 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15MktgF028489 ==============================End of - Headers==============================
I did not notice any separation for my work. I was use fairly high beam currents on an old W-gun SEM. I don't think I had the resolution to see any separation. Maybe someone could detect that with these new SEMs. I have not had occasion to go back and check.
If someone is interested in checking the stuff out, I still have some polymerized pellets or could prepare more. I still have iodoform on hand.
Warren S.
-----Original Message----- X-from: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com] Sent: Thursday, February 05, 2009 4:28 PM To: wesaia-at-iastate.edu
Years ago (1980s), I followed someone else's lead to dope epoxy resin with iodoform to increase its average atomic number.
I was studying minerals in coal and standard epoxy resins provided practically no contrast with coal in backscattered electron images. We ended up dissolving about 15 wt.% iodoform in epoxy resin. We later added the hardener and the epoxy behaved in much the same way as the original two-part epoxy. That is, hardness and polymerization were similar.
The resulting epoxy offered significant contrast with coal and allowed us to proceed with automated image analyses.
Be advised that iodoform is rather nasty and needs to be handled with care.
For what it's worth, I began working with iodoform shortly after the Right-to-know laws were passed. Suppliers had recently started including MSDSs with all their chemicals. I was still bemused that my chemical-grade calcium carbonate "should be disposed of in an approved, chemical-waste landfill" when my iodoform arrived. I had to do some more of my own research to determine if iodoform was really as nasty as the MSDS said or not. (It is.) Someone was crying wolf about the calcium carbonate while a contractor was laying tons of the stuff just outside our building as a base for the parking lot.
Warren S.
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Wednesday, January 28, 2009 1:03 PM To: wesaia-at-iastate.edu
Hi Jon,
We have an old Hitachi S-520 SEM still operational. Once or twice a year we are asked to look at paediatric hair specimens for defects. Of course, babies can't tell what's wrong with them so looking at hair samples can sometimes give you a clue as to what is the problem. Ie, the hair defect may be one part of a wider syndrome.
Regards,
John Brealey
Senior Medical Scientist, Electron Microscopy Unit
T 08 8222 6612 F 08 8222 6425
www.sapathology.sa.gov.au
SA Pathology (TQEH) Quality Pathology supporting Training and Research
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==============================Original Headers============================== 11, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 5 16:59:38 2009 11, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Mxapu015166 11, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:59:37 -0600 11, 27 -- X-IronPort-AV: E=Sophos;i="4.37,388,1231075800"; 11, 27 -- d="scan'208";a="9016862" 11, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88]) 11, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 06 Feb 2009 09:29:34 +1030 11, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 11, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id 11, 27 -- 8.1.263.0; Fri, 6 Feb 2009 09:30:20 +1030 11, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au 11, 27 -- (Postfix) with ESMTP id 2872534C13 for {Microscopy-at-microscopy.com} ; Fri, 6 11, 27 -- Feb 2009 09:29:34 +1030 (CST) 11, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 11, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 11, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 11, 27 -- Subject: Current Biological SEM 11, 27 -- Date: Fri, 6 Feb 2009 09:29:33 +1030 11, 27 -- Organization: IMVS 11, 27 -- Message-ID: {008d01c987e5$68ea35d0$5954150a-at-41347i} 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; charset="us-ascii" 11, 27 -- Content-Transfer-Encoding: 7bit 11, 27 -- X-Mailer: Microsoft Office Outlook 11 11, 27 -- Thread-Index: AcmH5WjGJuYRPqLnSJqOKySzQVOteA== 11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
As I replied to Jon (forgot to change the address to the server), we do a fair bit of SEM, quite a bit of that is cryoSEM, and mostly of plants, with a few insects now and then. The reason is to avoid extraction and other artefacts - dehydration causes irreversible extraction of lots of soluble material from tissues, esp. cell walls but also from plant vacuoles. We also do analysis of mainly light element distribution and quantity in specific tissues and cells. See recent review by McCully et al. (2009) Functional Plant Biology 36:97 for more details. CryoSEM is also very good for very soft material - for example, rotting tissues in which you want to look at the disease organism in situ - which would fall apart with any other preparation method.
As Tobias outlined, another couple of techniques we use much more these days are environmental SEM, now that this technology is more mature and reproducible, though still fiddly to get right at very low vacuum; and FESEM.
The entomologists still look at bee's knees and fly eyes, though they tend to look at details of the rear ends for taxonomic analysis.....
But I agree that with improvements to confocal imaging, and with superresolution imaging almost consumer-ready - STED and cousins, light microscopy will continue to explode, mainly because of the ability to differentially tag cell components and cell functions in a huge variety of ways.
cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 6/02/09 9:28 AM, "larry.ackerman-at-ucsf.edu" {larry.ackerman-at-ucsf.edu} wrote:
The perspective from an institution focused almost entirely on human medical and related biological research studies puts SEM in the far background. Whereas, there was once several SEM instruments on campus there is now only one old, mostly inaccesible instrument in the Dental School. I might hear an inquiry about SEM resources once a year and I have to direct those investigators to a lab at another institution that enjoys a full spectrum of departments and science fields. The vast majority of funding and interest primarily focuses on genetic and molecular questions. The SEM does not provide much useful data for these studies though it is invaluable for descriptive illustrations. I think this is supported by the dearth of SEM data in mamalian biology studies in high profile publications such as Science, Nature and Cell. In contrast light microscopy, particularly laser scanning confocal microscopy, is booming. I estimate that there are over 30 heavily used confocal systems at this university and light micrographs are commonly found in research publications. So as much as I pine for the good old days the reality that I experience is that SEM is a minor methodology in current biomedical research. -- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
We're in a similar situation to John. Most of our Diagnostic Pathology requests are for abnormalities associated with hair. Occasionally we also get some really interesting specimens for EDS for metals. E.g. Patients who self-prescribe homeopathic Silver solutions and end up with Agyria (the skin turns a silver blue colour...permanently!)
Regards
Naomi McCallum Supervising Scientist Electron Microscope Unit Anatomical Pathology & Cytopathology PATHOLOGY QUEENSLAND Central Laboratory RBWH
} } } {john.brealey-at-imvs.sa.gov.au} 6/02/2009 9:09 am } } }
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Hi Jon,
We have an old Hitachi S-520 SEM still operational. Once or twice a year we are asked to look at paediatric hair specimens for defects. Of course, babies can't tell what's wrong with them so looking at hair samples can sometimes give you a clue as to what is the problem. Ie, the hair defect may be one part of a wider syndrome.
Regards,
John Brealey
Senior Medical Scientist, Electron Microscopy Unit
T 08 8222 6612 F 08 8222 6425
www.sapathology.sa.gov.au
SA Pathology (TQEH) Quality Pathology supporting Training and Research
This email may contain confidential information, which also may be legally privileged. Only the intended recipient(s) may access, use, distribute or copy this e-mail. If this e-mail is received in error, please inform the sender by return e-mail and delete the original. If there are doubts about the validity of this message, please contact the sender by telephone. It is the recipient's responsibility to check the e-mail and any attached files for viruses.
==============================Original Headers============================== 11, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 5 16:59:38 2009 11, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15Mxapu015166 11, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:59:37 -0600 11, 27 -- X-IronPort-AV: E=Sophos;i="4.37,388,1231075800"; 11, 27 -- d="scan'208";a="9016862" 11, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88]) 11, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 06 Feb 2009 09:29:34 +1030 11, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 11, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id 11, 27 -- 8.1.263.0; Fri, 6 Feb 2009 09:30:20 +1030 11, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au 11, 27 -- (Postfix) with ESMTP id 2872534C13 for {Microscopy-at-microscopy.com} ; Fri, 6 11, 27 -- Feb 2009 09:29:34 +1030 (CST) 11, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 11, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 11, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 11, 27 -- Subject: Current Biological SEM 11, 27 -- Date: Fri, 6 Feb 2009 09:29:33 +1030 11, 27 -- Organization: IMVS 11, 27 -- Message-ID: {008d01c987e5$68ea35d0$5954150a-at-41347i} 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; charset="us-ascii" 11, 27 -- Content-Transfer-Encoding: 7bit 11, 27 -- X-Mailer: Microsoft Office Outlook 11 11, 27 -- Thread-Index: AcmH5WjGJuYRPqLnSJqOKySzQVOteA== 11, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 27 -- From prvs=1288c7ae5c=naomi_mccallum-at-health.qld.gov.au Fri Feb 6 00:05:21 2009 25, 27 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1665IPt018101 25, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 00:05:20 -0600 25, 27 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 25, 27 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id n16653s9005548 25, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 16:05:03 +1000 25, 27 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 25, 27 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 25, 27 -- with ESMTP id 25, 27 -- {T8c58be87fdc0a803351874-at-gwd-mail06.remote.health.qld.gov.au} for 25, 27 -- {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 16:05:03 +1000 25, 27 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 25, 27 -- Novell_GroupWise; Fri, 06 Feb 2009 16:05:03 +1000 25, 27 -- Message-Id: {498C5EC0.88BE.00AA.0-at-health.qld.gov.au} 25, 27 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 25, 27 -- Date: Fri, 06 Feb 2009 16:04:49 +1000 25, 27 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au} 25, 27 -- To: {Microscopy-at-microscopy.com} 25, 27 -- Subject: Re: [Microscopy] Current Biological SEM 25, 27 -- References: {200902052309.n15N9dP0015150-at-ns.microscopy.com} 25, 27 -- In-Reply-To: {200902052309.n15N9dP0015150-at-ns.microscopy.com} 25, 27 -- Mime-Version: 1.0 25, 27 -- Content-Type: text/plain; charset="us-ascii" 25, 27 -- Content-Disposition: inline 25, 27 -- Content-Transfer-Encoding: 8bit 25, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1665IPt018101 ==============================End of - Headers==============================
I believe we had similar discussion on this topic some years back, as it related to evidence in litigation. I believe the concensus is that it is okay to adjust images for presentation or publication purposes as long as the data that is represented isn't significantly altered to show something that it should not be. This is why we adjust the contrast and brightness of digital images, and even back in the old days of print photography we "burned and dogged" the print. We did this to compensate for shortcomings of the equipment and technique used to gather documentation. In the case of legal matters, weight is placed on testimony of the expert witness, not on the picture that was taken.
The real value of the image is in the research, viewing, and interpretation of what the investigator saw before taking the image. The investigator (hopefully) knows the limitations of the equipment, tweaked the instrument to look at the image in different ways, and came up with his conclusion long before taking the picture.
I don't think any reasonable person would chastise somebody's photographic evidence or scientific documentation because the contrast was altered or an errant blemish was covered up in the name of producing an eye-pleasing presentation.
Stu Smalinskas, P.E. Metallurgist Plymouth, Michigan SKF USA
--- On Thu, 2/5/09, sptinling-at-ucdavis.edu {sptinling-at-ucdavis.edu} wrote:
} From: sptinling-at-ucdavis.edu {sptinling-at-ucdavis.edu} } Subject: [Microscopy] Image Processing -- Ethics and Validity } To: smalinskas-at-yahoo.com } Date: Thursday, February 5, 2009, 5:15 PM } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } I am new here and this is my first response after } reading Netnotes in Microscopy Today for } years. Thanks for all the great } information. Please pardon any initial unintended errors } in protocol. } I am an old dog learning new tricks so I would } like members of this discussion to consider } reconciling a couple of concepts. 1. Materials } and Methods – complete reporting. 2. Significant } figures (mathematically speaking). } I was trained for EM and photography back in the } days of glass plates and drum dryers with } Pacasol. I now work in an age where we have } discarded all our film photography materials and } work with digital cameras and software. In the } old days, dogging and burning were standard } techniques in the dark room to get your EM } picture to look perfect. No white spots from } dust were allowed, and bits of stain in the lumen } of an open perfused blood vessel were eliminated } with a conveniently placed label and arrow. That } was never considered as altering the significance } of the data which the image conveyed and that was } by an instructor who always stressed complete } descriptions in the materials and methods section } of everything that was done, all equipment used, } etc. No one ever reported that the left corner } of Fig.1 was dogged for 3 seconds while printing } on grade 3 paper with the developer at 22 degrees } centigrade. I have done enough EM to know that } sometimes the picture you end up with has } aesthetic defects. I am equally sure that most } in not all the published EM (or LM, SEM, etc) } images have that conveniently placed arrow or } label somewhere. Should that have been reported } then and or now? How is it any different if you } remove what you know to be dirt or a stain } particle from an image by any digital subtraction } or addition or the rubber stamps method then by } placing that convenient label or arrow over it } and then merging the layers? You still have } changed pixel values. I personally do not see } the difference nor do I think it necessary to } state in the figure legend or an appendix all the } aesthetic alterations to an image. Also, I can } not image a journal allowing you to. Even with } online publishing I am constantly being told by } the journals to make things shorter. } } As for the data contained in the digital image, } the amount contained in individual pixel values } and pixels per image goes way beyond the number } of significant figures relevant to the data being } presented by the image. And before you even get } to Photoshop, the camera software lets you alter } the image intensity, number of bits in an image } (8, 12, 16) etc. To me it makes perfect sense to } have a dark and light background image (as } previously mentioned) and apply them to the image } in question to eliminate defects in the optical } path and report that in the M&M. However, if } the point of the picture in question depends on } the number of an individual pixel or the numbers } of pixels in an image, then it makes sense to } include any alterations in the M&M } section. However, I suspect that for a very } large percentage of the images published, a } rubber stamped alteration of a capillary lumen } for aesthetic purposes would be immaterial even } though it changed the original values of the } pixels in question. I would never consider that } scientific fraud. Even when the pixels in } question might have significance, when does the } issue of reporting significant figures come in to } the issue? I can accept that there are images } produced where the data from the image are the } pixel values. But, as I am mostly biologically } oriented that does not happen very often to } me. There are lots of papers out there in the } early days of immunohistochemistry which tried to } equate stain intensity (the number of a given } pixel) with amount of pathology (numbers of } pixels equal to or greater than a given pixel } value per image with the right RGB or CMY or HIS, } etc values) only to discover that the errors } derived from trying to mix those two parameters } yielded only false results. As my EM instructor } always pointed out, everything we report is an } “artifactâ€. So, if pixel values are that } important, when do we apply the test of } significant figures to the pixel data. If I } publish 1 picture from the skin of 1 test animal } showing “representative†damage to the basal } lamina in an experimental group (lets say 15 } control and 15 experimental animals) then anyone } who accepts my picture might further question the } M&M section, for number of images taken per } section, per sample, per animal and further ask } if the data were derived from a sterologic } process of sampling or whether I just got } lucky. I rarely see any of this data } reported. I realize as was said earlier that the } day is coming when there will be enough space on } the journal’s server for them to require that the } original unaltered image be placed there along } with the published one (in which you placed that } convenient arrow) but then how do we know that an } unaltered image was placed there in the first } place? We do not -- we trust in the integrity } within our community, and although fraud does } occur is it at such a rate that it is really an } issue? I suppose the well trained individual } could download the original of a “questionable†} published image and subject it to an analysis } which would show pixel alterations and that would } be good if one suspected deliberate fraud. My } guess is that it would be a very rare thing. To } close, I am a firm believer in complete M&M } reporting and that would include how I took my } image – camera, microscope, software, and } techniques used, but I can not see burdening } readers with the morally appropriate mea culpa } that I typed a figure label or placed an arrow } over an aesthetic defect and merged the layers to } make a picture more aesthetically pleasing, or } that I “rubberstamped†a piece of dirt. Thanks } for considering these issues. Steve } } Steven P. Tinling Ph.D. } Director of Research } Otolaryngology Research Lab } University of California, Davis } 1515 Newton Ct., Rm. 209 } Davis, CA. 95616-4859 } Phone: 530-754-5045 } Fax: 530-754-5046 } } } } ==============================Original } Headers============================== } 5, 22 -- From sptinling-at-ucdavis.edu Thu Feb 5 16:14:10 } 2009 } 5, 22 -- Received: from warsaw.ucdavis.edu } (warsaw.ucdavis.edu [128.120.32.41]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n15ME9O8011215 } 5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb } 2009 16:14:10 -0600 } 5, 22 -- Received: from MOTO-2951.ucdavis.edu } ([128.120.223.66]) } 5, 22 -- (authenticated bits=0) } 5, 22 -- by warsaw.ucdavis.edu } (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n15ME3sK026356 } 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } bits=256 verify=NO) } 5, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 Feb } 2009 14:14:04 -0800 (PST) } 5, 22 -- Message-Id: } {200902052214.n15ME3sK026356-at-warsaw.ucdavis.edu} } 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 5, 22 -- Date: Thu, 05 Feb 2009 14:14:28 -0800 } 5, 22 -- To: Microscopy-at-Microscopy.Com } 5, 22 -- From: Steve Tinling {sptinling-at-ucdavis.edu} } 5, 22 -- Subject: Image Processing -- Ethics and Validity } 5, 22 -- Mime-Version: 1.0 } 5, 22 -- Content-Type: text/plain; } charset="iso-8859-1"; format=flowed } 5, 22 -- X-Virus-Scanned: ClamAV version 0.93.3, } clamav-milter version 0.93.3 on av5 } 5, 22 -- X-Virus-Status: Clean } 5, 22 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 } 5, 22 -- Content-Transfer-Encoding: 8bit } 5, 22 -- X-MIME-Autoconverted: from quoted-printable to } 8bit by ns.microscopy.com id n15ME9O8011215 } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 23 -- From smalinskas-at-yahoo.com Fri Feb 6 07:50:19 2009 9, 23 -- Received: from web34405.mail.mud.yahoo.com (web34405.mail.mud.yahoo.com [66.163.178.154]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n16DoJ7D005023 9, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Fri, 6 Feb 2009 07:50:19 -0600 9, 23 -- Received: (qmail 32083 invoked by uid 60001); 6 Feb 2009 13:50:18 -0000 9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 23 -- s=s1024; d=yahoo.com; 9, 23 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 23 -- b=jQMlXOx1tr350MM3DeHYqfMh4xyxzHhsHYJHkn8472pz5jm2azP+6NIcq98D+VQkm8IGtWRB3SPrxy7Y1G1vUcxkDpyhtHzrmFCpP7+MUireuFEqkhcp5GI3B2NtzFl7rhW59LS4/xlm81dOGwVR343EgZTgV6rcSdmeQonsWEE=; 9, 23 -- X-YMail-OSG: M69shaoVM1ko3RemqbTIgpXlrpu2CHDz88FYhvYt.jTt_XrOUnsdTrSgX4jbFHCKqIrLxSvF6AsqMpFMqsNUcR09itz.p3kbCSFps0lkAS9Md0BmbpwQqzyJ28boR3aEfI3t4fsZv75hJ4x32F4lvLike59T8HyuCxokbQ_Me.QlG9jQtdgiGT0PBxWgLNwXbC4dOcT5_SXH3Om6iGKuqH0ug4Kzl3s- 9, 23 -- Received: from [141.151.33.213] by web34405.mail.mud.yahoo.com via HTTP; Fri, 06 Feb 2009 05:50:18 PST 9, 23 -- X-Mailer: YahooMailWebService/0.7.260.1 9, 23 -- Date: Fri, 6 Feb 2009 05:50:18 -0800 (PST) 9, 23 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 9, 23 -- Reply-To: smalinskas-at-yahoo.com 9, 23 -- Subject: Re: [Microscopy] Image Processing -- Ethics and Validity 9, 23 -- To: sptinling-at-ucdavis.edu, microscopy-at-ns.microscopy.com 9, 23 -- In-Reply-To: {200902052215.n15MFm6M015702-at-ns.microscopy.com} 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=utf-8 9, 23 -- Message-ID: {559072.30236.qm-at-web34405.mail.mud.yahoo.com} 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16DoJ7D005023 ==============================End of - Headers==============================
Hi, Good morning, I like to thank every one who respond to me and It would be a great help. I am new to this forum and it is wonderful and supportive forum for microscopy . I would like to introduce myself as my name is Kabilan V.Gounder working in UMDNJ-NJDS in New Jersey and i am running an EM facility in our school. I am happy to join in this group. Have nice day.
Sincerely V.Kabilan
==============================Original Headers============================== 3, 26 -- From velliyka-at-umdnj.edu Fri Feb 6 07:54:21 2009 3, 26 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n16DsLG2010495 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 07:54:21 -0600 3, 26 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 3, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 1AEDB4BE60 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 08:54:21 -0500 (EST) 3, 26 -- Received: from umdnj.edu (unknown [10.32.15.102]) 3, 26 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 6D47A4BEA0 3, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 08:54:06 -0500 (EST) 3, 26 -- Received: from ([10.32.15.171]) 3, 26 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.6261191; 3, 26 -- Fri, 06 Feb 2009 08:54:01 -0500 3, 26 -- MIME-version: 1.0 3, 26 -- Content-transfer-encoding: 7BIT 3, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed 3, 26 -- Received: from [10.4.62.229] ([10.32.15.102]) 3, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built 3, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KEN00I1TDA1VT70-at-umduwc02.umdnj.edu} for 3, 26 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 08:54:01 -0500 (EST) 3, 26 -- Message-id: {c2866f9cc5838564f89ef176565b8432-at-umdnj.edu} 3, 26 -- To: microscopy-at-microscopy.com 3, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu} 3, 26 -- Subject: Re:Scanner for plate film: Thanks 3, 26 -- Date: Thu, 05 Feb 2009 20:57:06 -0500 3, 26 -- X-Mailer: Apple Mail (2.624) ==============================End of - Headers==============================
I followed this thread on the list with great interest as I was setting up our new integrated imaging facility. I also chose the Epson perfection 750 and we're very satisfied with the results
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: Robert.Zonis-at-Sanford.com [mailto:Robert.Zonis-at-Sanford.com] Sent: Thursday, February 05, 2009 5:18 PM To: rjharris-at-uwo.ca
I prefer the Epson Perfection V750-M Pro Scanner. It costs around $700, but the extra money is worth it for the extra software and calibration standard that is included. It makes a pretty decent stand-in for a stereoscopic microscope, too.
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
-----Original Message-----
} I like to scan plate film (Philips EM300) , can you please suggest whic
} one is suitable for scaaning the EM films. } } Thank you very much for your help. } } V.Kabilan } UMDNJ-NJDS } Newark, USA
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==============================Original Headers============================== 11, 34 -- From Robert.Zonis-at-Sanford.com Thu Feb 5 16:09:50 2009 11, 34 -- Received: from mail142.messagelabs.com (mail142.messagelabs.com [216.82.249.99]) 11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15M9nuW004441 11, 34 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 16:09:50 -0600 11, 34 -- X-VirusChecked: Checked 11, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 11, 34 -- X-Msg-Ref: server-8.tower-142.messagelabs.com!1233871787!65085875!1 11, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 11, 34 -- X-Originating-IP: [198.176.16.25] 11, 34 -- Received: (qmail 12669 invoked from network); 5 Feb 2009 22:09:47 -0000 11, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25) 11, 34 -- by server-8.tower-142.messagelabs.com with RC4-SHA encrypted SMTP; 5 Feb 2009 22:09:47 -0000 11, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 11, 34 -- Thu, 5 Feb 2009 16:09:46 -0600 11, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 34 -- Content-class: urn:content-classes:message 11, 34 -- MIME-Version: 1.0 11, 34 -- Content-Type: text/plain; 11, 34 -- charset="us-ascii" 11, 34 -- Subject: RE: [Microscopy] Scanner for Plate film 11, 34 -- Date: Thu, 5 Feb 2009 16:07:49 -0600 11, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401950A46-at-naoaksasebe02.nr.ad.newellco.com} 11, 34 -- In-Reply-To: {200902052159.n15LxEks031584-at-ns.microscopy.com} 11, 34 -- X-MS-Has-Attach: 11, 34 -- X-MS-TNEF-Correlator: 11, 34 -- Thread-Topic: [Microscopy] Scanner for Plate film 11, 34 -- Thread-Index: AcmH3P7d8bcKCLdLQA+8rheHYN/2EQAAGMCA 11, 34 -- References: {200902052159.n15LxEks031584-at-ns.microscopy.com} 11, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 11, 34 -- To: {velliyka-at-umdnj.edu} 11, 34 -- Cc: {microscopy-at-microscopy.com} 11, 34 -- X-OriginalArrivalTime: 05 Feb 2009 22:09:46.0856 (UTC) FILETIME=[74C88E80:01C987DE] 11, 34 -- Content-Transfer-Encoding: 8bit 11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15M9nuW004441 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 28 -- From rjharris-at-uwo.ca Fri Feb 6 08:39:53 2009 21, 28 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16Edpwg001996 21, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 08:39:52 -0600 21, 28 -- MIME-version: 1.0 21, 28 -- Content-type: text/plain; charset=iso-8859-1 21, 28 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 21, 28 -- by zeppo.mail.uwo.pri 21, 28 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 21, 28 -- with ESMTP id {0KEN00B6AFEEBIH0-at-zeppo.mail.uwo.pri} for 21, 28 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 09:39:50 -0500 (EST) 21, 28 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 21, 28 -- by zeppo.mail.uwo.pri 21, 28 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 21, 28 -- with ESMTPSA id {0KEN001DEFEEB640-at-zeppo.mail.uwo.pri} for 21, 28 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 09:39:50 -0500 (EST) 21, 28 -- From: Richard Harris {rjharris-at-uwo.ca} 21, 28 -- To: Microscopy List {microscopy-at-microscopy.com} 21, 28 -- References: {200902052217.n15MHrsT021497-at-ns.microscopy.com} 21, 28 -- In-reply-to: {200902052217.n15MHrsT021497-at-ns.microscopy.com} 21, 28 -- Subject: RE: [Microscopy] RE: Scanner for Plate film 21, 28 -- Date: Fri, 06 Feb 2009 09:39:47 -0500 21, 28 -- Message-id: {003001c98868$c4d72460$4e856d20$-at-ca} 21, 28 -- X-Mailer: Microsoft Office Outlook 12.0 21, 28 -- Thread-index: AcmH35tQiGhrYILiR8KQNmcC3uzwYgAiNdzQ 21, 28 -- Content-language: en-us 21, 28 -- Content-Transfer-Encoding: 8bit 21, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16Edpwg001996 ==============================End of - Headers==============================
Weellll... I have to agree that SEM isn't used much in biomedical research, but disagree about why. SEM is not used much in biological work, biomedical in particular, but I think it is used more than indicated here, and that it is under used. I worked on many SEM biomedical/bioengineering projects at UW-Madison involving cultured cells, biofllms in catheters, implanted medical devices, bone fractures, and others. And using gold-conjugated antibodies to study cell-surface receptors. SEM is a much better method for this than TEM or confocal, as the entire cell surface can be sampled at high resolution, instead of just very thin cross-sectional slices that could easily miss the receptors, or instead of at light microscope resolution. Studies like this can raise and answer questions like: are the receptors distributed at random on the cell surface or in patterns? Is there any relation between receptor distribution and cell surface structures like ruffles, etc.? And so on. There are many biomedical molecular/genetic questions that could be addressed with SEM, there just are few people thinking of them. The problem isn't "how useful is the technique", but how people are thinking and how much they know about what techniques are available. Most molecular/genetic people know little or nothing of microscopy, or EM, much less SEM, so they never consider them useful techniques. That just means they don't know much about the technique, not that it's not useful.
Phl
} The perspective from an institution focused almost entirely on human } medical and related biological research studies puts SEM in the far } background. Whereas, there was once several SEM instruments on campus } there is now only one old, mostly inaccesible instrument in the Dental } School. I might hear an inquiry about SEM resources once a year and I } have to direct those investigators to a lab at another institution that } enjoys a full spectrum of departments and science fields. The vast } majority of funding and interest primarily focuses on genetic and } molecular questions. The SEM does not provide much useful data for these } studies though it is invaluable for descriptive illustrations. I think } this is supported by the dearth of SEM data in mamalian biology studies } in high profile publications such as Science, Nature and Cell. In } contrast light microscopy, particularly laser scanning confocal } microscopy, is booming. I estimate that there are over 30 heavily used } confocal systems at this university and light micrographs are commonly } found in research publications. So as much as I pine for the good old } days the reality that I experience is that SEM is a minor methodology in } current biomedical research. } -- } Larry Ackerman, Specialist } UCSF, Dept. of Anatomy, Rm S1347 } 513 Parnassus Ave., Box 0452 } San Francisco, CA 94143 } } larry.ackerman-at-ucsf.edu } } 415-476-4400 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 25 -- From oshel1pe-at-cmich.edu Fri Feb 6 09:28:37 2009 3, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FSatC017446 3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:28:36 -0600 3, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n16FSY48026412 3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 10:28:35 -0500 3, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 25 -- Fri, 6 Feb 2009 10:28:03 -0500 3, 25 -- Mime-Version: 1.0 3, 25 -- Message-Id: {f0624080dc5b204816b90-at-[141.209.160.249]} 3, 25 -- In-Reply-To: {200902052225.n15MPXlK005895-at-ns.microscopy.com} 3, 25 -- References: {200902052225.n15MPXlK005895-at-ns.microscopy.com} 3, 25 -- Date: Fri, 6 Feb 2009 10:28:00 -0500 3, 25 -- To: Microscopy-at-microscopy.com 3, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 25 -- Subject: [Microscopy] Re: Current biological SEM 3, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 25 -- X-OriginalArrivalTime: 06 Feb 2009 15:28:03.0112 (UTC) FILETIME=[803EDA80:01C9886F] 3, 25 -- X-Canit-CHI2: 0.00 3, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 3, 25 -- X-CanItPRO-Stream: default 3, 25 -- X-Canit-Stats-ID: 8545392 - d52e9df7ecec 3, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I agree with Phil on this, and it may be partially due to the EM community not reaching out to researchers with information about the capabilities of our techniques.
It's easy, for us at least, to fall into the routine of just reacting to the researchers coming through the door with their projects and a preconceived idea of the best way to approach them. We often have clients come in with completely inappropriate protocols that they want us to follow, simply because they have seen them in a journal article and believe that they are universally applicable. Quite often they may be completely uninformed about other, more appropriate, techniques.
Being a bit more proactive in getting information out in the form of workshops, brown bag lectures, newsletters, etc., might do wonders in renewing interest in things like use of SEM technologies in biological research.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Friday, February 06, 2009 9:30 AM To: Tindall, Randy D.
Weellll... I have to agree that SEM isn't used much in biomedical research, but disagree about why. SEM is not used much in biological work, biomedical in particular, but I think it is used more than indicated here, and that it is under used. I worked on many SEM biomedical/bioengineering projects at UW-Madison involving cultured cells, biofllms in catheters, implanted medical devices, bone fractures, and others. And using gold-conjugated antibodies to study cell-surface receptors. SEM is a much better method for this than TEM or confocal, as the entire cell surface can be sampled at high resolution, instead of just very thin cross-sectional slices that could easily miss the receptors, or instead of at light microscope resolution. Studies like this can raise and answer questions like: are the receptors distributed at random on the cell surface or in patterns? Is there any relation between receptor distribution and cell surface structures like ruffles, etc.? And so on. There are many biomedical molecular/genetic questions that could be addressed with SEM, there just are few people thinking of them. The problem isn't "how useful is the technique", but how people are thinking and how much they know about what techniques are available. Most molecular/genetic people know little or nothing of microscopy, or EM, much less SEM, so they never consider them useful techniques. That just means they don't know much about the technique, not that it's not useful.
Phl
} The perspective from an institution focused almost entirely on human } medical and related biological research studies puts SEM in the far } background. Whereas, there was once several SEM instruments on campus } there is now only one old, mostly inaccesible instrument in the Dental } School. I might hear an inquiry about SEM resources once a year and I } have to direct those investigators to a lab at another institution that } enjoys a full spectrum of departments and science fields. The vast } majority of funding and interest primarily focuses on genetic and } molecular questions. The SEM does not provide much useful data for these } studies though it is invaluable for descriptive illustrations. I think } this is supported by the dearth of SEM data in mamalian biology studies } in high profile publications such as Science, Nature and Cell. In } contrast light microscopy, particularly laser scanning confocal } microscopy, is booming. I estimate that there are over 30 heavily used } confocal systems at this university and light micrographs are commonly } found in research publications. So as much as I pine for the good old } days the reality that I experience is that SEM is a minor methodology in } current biomedical research. } -- } Larry Ackerman, Specialist } UCSF, Dept. of Anatomy, Rm S1347 } 513 Parnassus Ave., Box 0452 } San Francisco, CA 94143 } } larry.ackerman-at-ucsf.edu } } 415-476-4400 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 25 -- From oshel1pe-at-cmich.edu Fri Feb 6 09:28:37 2009 3, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FSatC017446 3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:28:36 -0600 3, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n16FSY48026412 3, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 10:28:35 -0500 3, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 25 -- Fri, 6 Feb 2009 10:28:03 -0500 3, 25 -- Mime-Version: 1.0 3, 25 -- Message-Id: {f0624080dc5b204816b90-at-[141.209.160.249]} 3, 25 -- In-Reply-To: {200902052225.n15MPXlK005895-at-ns.microscopy.com} 3, 25 -- References: {200902052225.n15MPXlK005895-at-ns.microscopy.com} 3, 25 -- Date: Fri, 6 Feb 2009 10:28:00 -0500 3, 25 -- To: Microscopy-at-microscopy.com 3, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 25 -- Subject: [Microscopy] Re: Current biological SEM 3, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 25 -- X-OriginalArrivalTime: 06 Feb 2009 15:28:03.0112 (UTC) FILETIME=[803EDA80:01C9886F] 3, 25 -- X-Canit-CHI2: 0.00 3, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 3, 25 -- X-CanItPRO-Stream: default 3, 25 -- X-Canit-Stats-ID: 8545392 - d52e9df7ecec 3, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 30 -- From TindallR-at-missouri.edu Fri Feb 6 09:43:05 2009 17, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 17, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16Fh4E7031692 17, 30 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:43:04 -0600 17, 30 -- X-IronPort-Anti-Spam-Filtered: true 17, 30 -- X-IronPort-Anti-Spam-Result: ApoEAFPpi0nRauUo/2dsb2JhbADBGgEJhSGIR4JhgTUGgjODIg 17, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 17, 30 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 06 Feb 2009 09:43:04 -0600 17, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 30 -- Fri, 6 Feb 2009 09:43:04 -0600 17, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 30 -- Content-class: urn:content-classes:message 17, 30 -- MIME-Version: 1.0 17, 30 -- Content-Type: text/plain; 17, 30 -- charset="us-ascii" 17, 30 -- Subject: RE: [Microscopy] Re: Current biological SEM 17, 30 -- Date: Fri, 6 Feb 2009 09:43:02 -0600 17, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7D9D-at-UM-XMAIL08.um.umsystem.edu} 17, 30 -- In-Reply-To: {200902061529.n16FTl07019090-at-ns.microscopy.com} 17, 30 -- X-MS-Has-Attach: 17, 30 -- X-MS-TNEF-Correlator: 17, 30 -- Thread-Topic: [Microscopy] Re: Current biological SEM 17, 30 -- Thread-Index: AcmIb79qbAkuuFpFRZuZFqNg7e4BJwAAElvg 17, 30 -- References: {200902061529.n16FTl07019090-at-ns.microscopy.com} 17, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 17, 30 -- To: {oshel1pe-at-cmich.edu} 17, 30 -- Cc: {microscopy-at-microscopy.com} 17, 30 -- X-OriginalArrivalTime: 06 Feb 2009 15:43:04.0472 (UTC) FILETIME=[997F7980:01C98871] 17, 30 -- Content-Transfer-Encoding: 8bit 17, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16Fh4E7031692 ==============================End of - Headers==============================
While brightness, contrast and gamma are fine to adjust. I would not feel comfortable in modifying the image to "cover up" a blemish. Assuming you are using a rubber stamp tool or something similar, you are modifying the information in the original image. You are then changing the information content of the picture. In my (not so humble) opinion, this is a dangerous step to take.
Cheers, Henk
At 08:52 AM 02/06/09, you wrote:
} {...snip...} } I don't think any reasonable person would chastise somebody's } photographic evidence or scientific documentation because the } contrast was altered or an errant blemish was covered up in the name } of producing an eye-pleasing presentation. } } Stu Smalinskas, P.E. } Metallurgist } Plymouth, Michigan } SKF USA
{...snip...}
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 12, 26 -- From colijn.1-at-osu.edu Fri Feb 6 09:47:49 2009 12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FlnoU009705 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:47:49 -0600 12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 12, 26 -- id {01N56C78QW7K8XXAO4-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 12, 26 -- Fri, 06 Feb 2009 10:47:48 -0500 (EST) 12, 26 -- Received: from HOC1.ecr6.ohio-state.edu 12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 12, 26 -- (PMDF V6.3-x18 #31556) 12, 26 -- with ESMTPA id {01N56C77W4LO8XWYNV-at-ecr6.ohio-state.edu} ; Fri, 12, 26 -- 06 Feb 2009 10:47:47 -0500 (EST) 12, 26 -- Date: Fri, 06 Feb 2009 10:48:34 -0500 12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 12, 26 -- Subject: Re: [Microscopy] Re: Image Processing -- Ethics and Validity 12, 26 -- In-reply-to: {200902061352.n16DqFVg007329-at-ns.microscopy.com} 12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 12, 26 -- To: smalinskas-at-yahoo.com 12, 26 -- Cc: Microscopy-at-microscopy.com 12, 26 -- Message-id: {01N56C77YSNY8XWYNV-at-ecr6.ohio-state.edu} 12, 26 -- MIME-version: 1.0 12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 12, 26 -- References: {200902061352.n16DqFVg007329-at-ns.microscopy.com} ==============================End of - Headers==============================
From primasa-at-silosprima.com.ar Fri Feb 6 11:27:25 2009 Return-Path: {primasa-at-silosprima.com.ar} Received: from google.com (78-141-65-141.minet.sk [78.141.65.141] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16HROAE000333 for {microscopylistserverarchive-at-microscopy.com} ; Fri, 6 Feb 2009 11:27:25 -0600 Received: from [80.99.105.175] (HELO google.com) by striped-sap.cn; Fri, 6 Feb 2009 18:27:06 +0100
Dear Listers Has anyone used Image J and a Nikon Coolpix to acquire images to a computer??? I am looking for an inexpensive image capture solution for my Microscopy, Imaging and Analysis course and this combo (if it works) looks like it would fit the bill...
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
==============================Original Headers============================== 7, 27 -- From rjharris-at-uwo.ca Fri Feb 6 14:43:00 2009 7, 27 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16KgxXN020552 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 14:42:59 -0600 7, 27 -- MIME-version: 1.0 7, 27 -- Content-type: text/plain; charset=iso-8859-1 7, 27 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39]) 7, 27 -- by harpo.mail.uwo.pri 7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 27 -- with ESMTP id {0KEN00JJ3W7NJNE0-at-harpo.mail.uwo.pri} for 7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST) 7, 27 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 7, 27 -- by harpo.mail.uwo.pri 7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 27 -- with ESMTPSA id {0KEN00FUMW7NCN00-at-harpo.mail.uwo.pri} for 7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST) 7, 27 -- From: Richard Harris {rjharris-at-uwo.ca} 7, 27 -- To: Microscopy List Server {microscopy-at-microscopy.com} , 7, 27 -- MSA Listserver {Microscopy-at-MSA.Microscopy.Com} 7, 27 -- Subject: Nikon coolpix and Image J 7, 27 -- Date: Fri, 06 Feb 2009 15:43:01 -0500 7, 27 -- Message-id: {003401c9889b$80b14760$8213d620$-at-ca} 7, 27 -- X-Mailer: Microsoft Office Outlook 12.0 7, 27 -- Thread-index: AcmIm4CIq+YuzxOzQbugKKhlmR1E0g== 7, 27 -- Content-language: en-us 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16KgxXN020552 ==============================End of - Headers==============================
I just changed the filament for Philips EM300 and saturated, the illumination looks good and I observe the samples. But the problem is when I develop the negatives, it looks empty. There was no image, Please let me know what is the problem and why the film is empty. Thanks for your help.
Kabilan UMDNJ-NJDS Newark, NJ, USA
==============================Original Headers============================== 4, 28 -- From velliyka-at-umdnj.edu Fri Feb 6 14:53:08 2009 4, 28 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n16Kr7Yo001511 4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 14:53:08 -0600 4, 28 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 4, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id DE1A24BE74 4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:53:06 -0500 (EST) 4, 28 -- Received: from umdnj.edu (unknown [10.32.15.102]) 4, 28 -- by zix02.umdnj.edu (Proprietary) with ESMTP id E3D1C4BE7C 4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:51:10 -0500 (EST) 4, 28 -- Received: from ([10.32.15.171]) 4, 28 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.6590949; 4, 28 -- Fri, 06 Feb 2009 15:50:41 -0500 4, 28 -- MIME-version: 1.0 4, 28 -- Content-transfer-encoding: 7BIT 4, 28 -- Content-type: text/plain; charset=US-ASCII; format=flowed 4, 28 -- Received: from [10.4.62.229] ([10.32.15.102]) 4, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built 4, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KEN00KD5WKHMN20-at-umduwc02.umdnj.edu} for 4, 28 -- Microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:50:41 -0500 (EST) 4, 28 -- In-reply-to: {20050228.151138.2728.0.fgask99200-at-juno.com} 4, 28 -- References: {20050228.151138.2728.0.fgask99200-at-juno.com} 4, 28 -- Message-id: {a49997ced5731f407b847097f4ccecf1-at-umdnj.edu} 4, 28 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu} 4, 28 -- Subject: RE: Philips EM300 plate film: no image on negatives 4, 28 -- Date: Fri, 06 Feb 2009 03:53:48 -0500 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- X-Mailer: Apple Mail (2.624) ==============================End of - Headers==============================
Rick, There is a Twain plugin for ImageJ, that should work with the most common interfaces (...is there a Nikon Twain Driver?). Also, if you save your images to the harddrive, like with Nikon Transfer or your camera software, you can import and batch import the images into ImageJ.
Joachim
-----Original Message----- X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca] Sent: Friday, February 06, 2009 2:53 PM To: Joachim Siegmund
Dear Listers Has anyone used Image J and a Nikon Coolpix to acquire images to a computer??? I am looking for an inexpensive image capture solution for my Microscopy, Imaging and Analysis course and this combo (if it works) looks like it would fit the bill...
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
==============================Original Headers============================== 7, 27 -- From rjharris-at-uwo.ca Fri Feb 6 14:43:00 2009 7, 27 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16KgxXN020552 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 14:42:59 -0600 7, 27 -- MIME-version: 1.0 7, 27 -- Content-type: text/plain; charset=iso-8859-1 7, 27 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39]) 7, 27 -- by harpo.mail.uwo.pri 7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 27 -- with ESMTP id {0KEN00JJ3W7NJNE0-at-harpo.mail.uwo.pri} for 7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST) 7, 27 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 7, 27 -- by harpo.mail.uwo.pri 7, 27 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 27 -- with ESMTPSA id {0KEN00FUMW7NCN00-at-harpo.mail.uwo.pri} for 7, 27 -- microscopy-at-microscopy.com; Fri, 06 Feb 2009 15:42:59 -0500 (EST) 7, 27 -- From: Richard Harris {rjharris-at-uwo.ca} 7, 27 -- To: Microscopy List Server {microscopy-at-microscopy.com} , 7, 27 -- MSA Listserver {Microscopy-at-MSA.Microscopy.Com} 7, 27 -- Subject: Nikon coolpix and Image J 7, 27 -- Date: Fri, 06 Feb 2009 15:43:01 -0500 7, 27 -- Message-id: {003401c9889b$80b14760$8213d620$-at-ca} 7, 27 -- X-Mailer: Microsoft Office Outlook 12.0 7, 27 -- Thread-index: AcmIm4CIq+YuzxOzQbugKKhlmR1E0g== 7, 27 -- Content-language: en-us 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16KgxXN020552 ==============================End of - Headers==============================
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==============================Original Headers============================== 20, 29 -- From jsiegmund-at-7thwavelabs.com Fri Feb 6 15:10:08 2009 20, 29 -- Received: from mail2.7thwavelabs.com (mail.7thwavelabs.com [66.49.5.136]) 20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16LA8c3016319 20, 29 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:10:08 -0600 20, 29 -- Received: from mail2.7thwavelabs.com (unknown [127.0.0.1]) 20, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 0480839800A; 20, 29 -- Fri, 6 Feb 2009 15:10:08 -0600 (CST) 20, 29 -- X-AuditID: c0a80218-a303abb000000bbe-fc-498ca72f4a8b 20, 29 -- Received: from wave-mail.7thwave.local (wave-mail.7thwave.local [192.168.2.29]) 20, 29 -- by mail2.7thwavelabs.com (Symantec Mail Security) with ESMTP id 8E5C3424013; 20, 29 -- Fri, 6 Feb 2009 15:10:07 -0600 (CST) 20, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 29 -- Content-class: urn:content-classes:message 20, 29 -- MIME-Version: 1.0 20, 29 -- Content-Type: text/plain; 20, 29 -- charset="iso-8859-1" 20, 29 -- Subject: RE: [Microscopy] Nikon coolpix and Image J 20, 29 -- Date: Fri, 6 Feb 2009 15:10:07 -0600 20, 29 -- Message-ID: {62A8156F8071C8439080D626DF8C33A660B294-at-wave-mail.7thwave.local} 20, 29 -- X-MS-Has-Attach: 20, 29 -- X-MS-TNEF-Correlator: 20, 29 -- Thread-Topic: [Microscopy] Nikon coolpix and Image J 20, 29 -- Thread-Index: AcmInPMN3IKszekdQmOcIyZ8l0IoRwAAUX8g 20, 29 -- References: {200902062053.n16KrLgG002039-at-ns.microscopy.com} 20, 29 -- From: "Joachim Siegmund" {jsiegmund-at-7thwavelabs.com} 20, 29 -- To: {rjharris-at-uwo.ca} , {microscopy-at-microscopy.com} 20, 29 -- X-Brightmail-Tracker: AAAAAA== 20, 29 -- Content-Transfer-Encoding: 8bit 20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16LA8c3016319 ==============================End of - Headers==============================
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==============================Original Headers============================== 14, 27 -- From jd-at-laddresearch.com Fri Feb 6 15:23:42 2009 14, 27 -- Received: from bean.electric.net (bean.electric.net [72.35.23.29]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16LNgaH030503 14, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 15:23:42 -0600 14, 27 -- Received: from 1LVYAm-0001jM-WB by bean.electric.net with emc1-ok (Exim 4.69) 14, 27 -- (envelope-from {jd-at-laddresearch.com} ) 14, 27 -- id 1LVYAn-0001kG-U3; Fri, 06 Feb 2009 13:23:41 -0800 14, 27 -- Received: by emcmailer; Fri, 06 Feb 2009 13:23:41 -0800 14, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 14, 27 -- by bean.electric.net with esmtps (TLSv1:AES256-SHA:256) 14, 27 -- (Exim 4.69) 14, 27 -- (envelope-from {jd-at-laddresearch.com} ) 14, 27 -- id 1LVYAm-0001jM-WB; Fri, 06 Feb 2009 13:23:41 -0800 14, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 27 -- Date: Fri, 06 Feb 2009 16:23:38 -0500 14, 27 -- To: r.sims-at-auckland.ac.nz 14, 27 -- From: jd {jd-at-laddresearch.com} 14, 27 -- Subject: Re: [Microscopy] Carbon Rods 14, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 14, 27 -- In-Reply-To: {200902031925.n13JPuFe021262-at-ns.microscopy.com} 14, 27 -- References: {200902031925.n13JPuFe021262-at-ns.microscopy.com} 14, 27 -- Mime-Version: 1.0 14, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 14, 27 -- X-Outbound-IP: 216.204.198.170 14, 27 -- X-Env-From: jd-at-laddresearch.com 14, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 14, 27 -- Message-Id: {E1LVYAn-0001kG-U3-at-bean.electric.net} ==============================End of - Headers==============================
} } I just changed the filament for Philips EM300 and saturated, the } illumination looks good and I observe the samples. But the problem is } when I develop the negatives, it looks empty. There was no image, } Please let me know what is the problem and why the film is empty. } Thanks for your help.
Hello,
I'm sure that more than a few of us have had a similar experience. Many different situations may cause this. Here are some things to check:
1. Were the negatives put into the holder facing in the correct direction (emulsion side up)? Recentley, someone remarked that the negative sheets have been incorrectly notched so that (if you use the notch for orientation), that may be the problem. Best way to check is to take one holder into the light and see if the film was put in properly.
2. Are the developer and fixer still active (not exhausted)? If in doubt, prepare new solutions of everything.
3. Is the shutter working in the TEM? This is either a mechanical or magnetic deflector. Best way to check is to keep the viewing screen down and press the exposure button to see if illumination is projected onto the viewing screen for the proper amount of time.
4. Is the exposure meter set properly in the TEM? If unsure, try different sensitivity settings.
I'm sure others will have even more ideas, but this will get you started.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I too, am looking for a new supply of 1/8" carbon rods that don't require such high currents, and therefore produce such high heat loads, compared to the "older" ones that I have left. These older ones only need about 16-18 amps to evaporate from a 3mm long x 0.8mm diameter tip that I sharpen on it. These low-current rods are a very dark black, and I think a bit softer than the lighter gray silvery ones that I also have and which take the high currents to evaporate. This later type seems to be all that's available out there now.
In fact, I contacted John at Ladd about a year ago and he told me those black ones, which were originally purchased from Ladd years ago (Ladd part # 30267), are no longer available. I would ask him again now what kind of carbon rods is he talking about that only take 18-20 amps as he mentioned?
I have a small stash of the black ones left, so I hope to find some more before I run out. I did try to contact the McMaster-Carr company about their carbon rods. The smallest ones they have are too big in diameter for our EM applications, but I never got a response back from them.
Gib
-- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
jd-at-laddresearch.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Richie, } } Based on our experience graphite rods do require about 18 to 20 amps } of current and a good vacuum. } } We also think that the stub and the carbon point must be of the same } material for optimum results. } } Regards, } John Arnott } } Disclaimer: Ladd Research distributes EM supplies including carbon rods } } Ladd Research } 83 Holly Court } Williston, VT 05495 } } On-line Catalog: www.laddresearch.com } } Telephone: 1-802-658-4961 (anywhere) } Toll Free 1-800-451-3406 (US) } Fax: 1-802-660-8859 } } e-mail: sales-at-laddresearch.com } } At 02:25 PM 2/3/2009, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi } } } } I've been having trouble with 3mm/1/8"carbon rods for my Edwards 306 } } coater since running } } out of my stash of Union Carbide National Carbon Company } } "Spectroscopic Electrodes" a } } while ago. } } } } I don't have a reliably calibrated vacuum gauge in the 306, but I } } suspect that the vacuum } } achieved isn't that great, however, it's as good as it was when I } } was getting great coating } } from the previous rods. } } I bought some "graphite" rods from one supplier, but they needed a } } higher current and } } temperature than the 306 could achieve. I then bought some } } "amorphous carbon" rods, but } } the success rate isn't very high, there just doesn't seem to be much } } coating produced. } } } } As I understand it, all rods contain a greater or lesser ratio of } } graphite and amorphous } } carbon, and the greater the graphite content, the higher the } } temperature required for coating. } } } } Does anyone know of a supplier of rods identical or similar to the } } old Union Carbide National } } Carbon Company ones? } } } } cheers } } Ritchie } } } } -- } } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } } Microanalyst Fax : 64 9 3737435 } } Department of Geology email : r.sims-at-auckland.ac.nz } } The University of Auckland } } Private Bag 92019 } } Auckland } } New Zealand
==============================Original Headers============================== 8, 22 -- From ahlst007-at-umn.edu Fri Feb 6 16:37:24 2009 8, 22 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16MbNV3028220 8, 22 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Feb 2009 16:37:23 -0600 8, 22 -- Received: from x-160-94-173-220.cbs.umn.edu (x-160-94-173-220.cbs.umn.edu [160.94.173.220]) 8, 22 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP 8, 22 -- Fri, 6 Feb 2009 16:37:17 -0600 (CST) 8, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-220.cbs.umn.edu [160.94.173.220] #+LO+TS+AU 8, 22 -- X-Umn-Classification: local 8, 22 -- Message-ID: {498CBBE3.9080603-at-umn.edu} 8, 22 -- Date: Fri, 06 Feb 2009 16:38:27 -0600 8, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 8, 22 -- Reply-To: ahlst007-at-umn.edu 8, 22 -- Organization: Imaging Center UM 8, 22 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 8, 22 -- MIME-Version: 1.0 8, 22 -- To: Microscopy-at-Microscopy.com 8, 22 -- Subject: Re: [Microscopy] Re: Carbon Rods 8, 22 -- References: {200902062125.n16LPxGR002965-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {200902062125.n16LPxGR002965-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi all, I'm sure Henk is absolutely right. The line between science and advertising or entertainment may be more accessible at a computer console than in a darkroom, but it is no less clear.
I think we should be able to debate when it is permissible to cross that line, recognising that sometimes it is almost impossible not to, rather than attempting to re-position it to a more convenient location.
A label always obscures information, but after all it has to be somewhere. Its placement may in some circumstances be dependent upon the conscience of the author. Placing a lable over gold particles in an immunostained "negative control" section would be clearly misleading, covering a small "irrelevant" blemish would be seen as allowable by almost everyone.
Rubberstamping, however is intrinsically, actively, misleading.
cheers Sally
Sally Stowe Centre for Visual Sciences, ANU
On Sat, February 7, 2009 2:47 am, colijn.1-at-osu.edu wrote: }
} } } ------------------------------------------------------------------------- } --- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } } Hi all, } } } While brightness, contrast and gamma are fine to adjust. I would not } feel comfortable in modifying the image to "cover up" a blemish. Assuming } you are using a rubber stamp tool or something similar, you are modifying } the information in the original image. You are then changing the } information content of the picture. In my (not so humble) opinion, this } is a dangerous step to take. } } Cheers, } Henk } } } } } At 08:52 AM 02/06/09, you wrote: } } } } } {...snip...} } } I don't think any reasonable person would chastise somebody's } } photographic evidence or scientific documentation because the contrast } } was altered or an errant blemish was covered up in the name of producing } } an eye-pleasing presentation. } } } } Stu Smalinskas, P.E. } } Metallurgist } } Plymouth, Michigan } } SKF USA } } } } {...snip...} } } } } Hendrik O. Colijn colijn.1-at-osu.edu } Campus Electron Optics Facility Ohio State University } (614) 292-0674 http://www.ceof.ohio-state.edu } Time is that quality of nature which keeps events from happening all } at once. Lately it doesn't seem to be working. } } } ==============================Original } Headers============================== } 12, 26 -- From colijn.1-at-osu.edu Fri Feb 6 09:47:49 2009 } 12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU } (er6s1.ecr6.ohio-state.edu [164.107.76.2]) } 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n16FlnoU009705 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 } 09:47:49 -0600 } 12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by } 12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) } 12, 26 -- id {01N56C78QW7K8XXAO4-at-ecr6.ohio-state.edu} for } Microscopy-at-microscopy.com; } 12, 26 -- Fri, 06 Feb 2009 10:47:48 -0500 (EST) } 12, 26 -- Received: from HOC1.ecr6.ohio-state.edu } 12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by } er6s1.ecr6.ohio-state.edu 12, 26 -- (PMDF V6.3-x18 #31556) } 12, 26 -- with ESMTPA id {01N56C77W4LO8XWYNV-at-ecr6.ohio-state.edu} ; Fri, } 12, 26 -- 06 Feb 2009 10:47:47 -0500 (EST) } 12, 26 -- Date: Fri, 06 Feb 2009 10:48:34 -0500 } 12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} } 12, 26 -- Subject: Re: [Microscopy] Re: Image Processing -- Ethics and } Validity } 12, 26 -- In-reply-to: {200902061352.n16DqFVg007329-at-ns.microscopy.com} } 12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu } 12, 26 -- To: smalinskas-at-yahoo.com } 12, 26 -- Cc: Microscopy-at-microscopy.com } 12, 26 -- Message-id: {01N56C77YSNY8XWYNV-at-ecr6.ohio-state.edu} } 12, 26 -- MIME-version: 1.0 } 12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed } 12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu } 12, 26 -- References: {200902061352.n16DqFVg007329-at-ns.microscopy.com} } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 13, 25 -- From sally.stowe-at-anu.edu.au Fri Feb 6 16:42:26 2009 13, 25 -- Received: from mail.rsbs.anu.edu.au (mail.rsbs.anu.edu.au [150.203.72.70]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16MgPSV007505 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 16:42:26 -0600 13, 25 -- Received: from mail.rsbs.anu.edu.au (localhost.localdomain [127.0.0.1]) 13, 25 -- by mail.rsbs.anu.edu.au (Postfix) with ESMTP id 1F1C935C20C 13, 25 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 09:42:16 +1100 (EST) 13, 25 -- Received: from 202.45.99.119 13, 25 -- (SquirrelMail authenticated user a274988) 13, 25 -- by mail.rsbs.anu.edu.au with HTTP; 13, 25 -- Sat, 7 Feb 2009 09:42:16 +1100 (EST) 13, 25 -- Message-ID: {3050.202.45.99.119.1233960136.squirrel-at-mail.rsbs.anu.edu.au} 13, 25 -- In-Reply-To: {200902061547.n16FlvsM009979-at-ns.microscopy.com} 13, 25 -- References: {200902061547.n16FlvsM009979-at-ns.microscopy.com} 13, 25 -- Date: Sat, 7 Feb 2009 09:42:16 +1100 (EST) 13, 25 -- Subject: Re: Image Processing -- Ethics and Validity 13, 25 -- From: Sally.Stowe-at-anu.edu.au 13, 25 -- To: microscopy-at-microscopy.com 13, 25 -- User-Agent: SquirrelMail/1.5.1 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-Type: text/plain;charset=iso-8859-1 13, 25 -- Content-Transfer-Encoding: 8bit 13, 25 -- X-RSBS-MailScanner-Information: Please contact the ISP for more information 13, 25 -- X-RSBS-MailScanner: Found to be clean 13, 25 -- X-MailScanner-From: sally.stowe-at-anu.edu.au ==============================End of - Headers==============================
Interesting discussion. I remember, quite well, the dark/black carbon rods from Union Carbide. In fact, I checked out our stock and we have several of the dark/black rods, which are softer and have the appearence of being "dusted" with a black coating. Maybe they are more crumbly and this represents surface material being shed. The majority of our stock, UltraCarbon, is very hard, silver/gray in appearance and has a slick surface. I know these require a lot of current to evaporate.
Have you evaluated the braided carbon strands for use in your application? We have been using them for some time now to produce carbon coatings to support plastic substrates, to strengthen sections and for conductivity coatings in X-ray analytical studies.They seem to be OK but I have not compared the quality of the coatings to the dark/black carbon. Something to add to my to-do list.
JB
} I too, am looking for a new supply of 1/8" } carbon rods that don't require such high } currents, and therefore produce such high heat } loads, compared to the "older" ones that I have } left. These older ones only need about 16-18 } amps to evaporate from a 3mm long x 0.8mm } diameter tip that I sharpen on it. These } low-current rods are a very dark black, and I } think a bit softer than the lighter gray silvery } ones that I also have and which take the high } currents to evaporate. This later type seems to } be all that's available out there now. } } In fact, I contacted John at Ladd about a year } ago and he told me those black ones, which were } originally purchased from Ladd years ago (Ladd } part # 30267), are no longer available. I would } ask him again now what kind of carbon rods is he } talking about that only take 18-20 amps as he } mentioned? } } I have a small stash of the black ones left, so } I hope to find some more before I run out. I did } try to contact the McMaster-Carr company about } their carbon rods. The smallest ones they have } are too big in diameter for our EM applications, } but I never got a response back from them. }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Hans; What is the frequency of the field? 10mGauss should strike fear in your heart. Around trains, the high field can accompany the arrival of the train, and the effect it has on the image is that the whole microscope goes out of alignment. Best to look for a different building! If you get bludgeoned by management, try a Spicer field cancellation system:
http://www.spicerconsulting.com/index.htm
they are represented in the Netherlands by
Elektronen-Optik-Service GmbH Zum Lonnenhohl 46 44319 Dortmund GERMANY
Tel: +49 (0)231 722 11 22
http://www.eos-do.de/
I know that our FEI FIBs have Spicer systems built in, so FEI may also be able to supply a system. Remember that these systems do not eliminate varying magnetic fields; they attenuate them. They are also frequency dependent.
John Mardinly, Numonyx
-----Original Message----- X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl] Sent: Thursday, February 05, 2009 8:19 AM To: MARDINLY, A
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] How to get rid of a magnetic field
Question: We want to move our EMís to a building close to tram tracks. At pre-installation measurements by FEI a distorting field of 10 mGauss (4.4 mGauss is the limit for a Tecnai12) was found. This building was a former MRI location; in the walls are steel (2cm thick) plating and a copper Faraday cage both with large holes in the ceiling. Can you give me advice on preventing this magnetic field? Is restoring the Faraday cage and/or the steel cage enough? Or do we need a mu-ferro or a Helmholtz cage? What will be the approximate costs of these?
==============================Original Headers============================== 9, 13 -- From zaluzec-at-microscopy.com Thu Feb 5 10:01:09 2009 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n15G17MS011832 9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 5 Feb 2009 10:01:09 -0600 9, 13 -- Mime-Version: 1.0 9, 13 -- Message-Id: {p06240800c5b0bdaf9cbc-at-[206.69.208.22]} 9, 13 -- Date: Thu, 5 Feb 2009 10:01:15 -0600 9, 13 -- To: microscopy-at-microscopy.com 9, 13 -- From: j.janssen-at-nki.nl (by way of MicroscopyListserver) 9, 13 -- Subject: viaWWW: How to get rid of a magnetic field 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 9, 13 -- Content-Transfer-Encoding: 8bit 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n15G17MS011832 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 29 -- From A.MARDINLY-at-numonyx.com Fri Feb 6 17:54:34 2009 24, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16NsYBM006872 24, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Feb 2009 17:54:34 -0600 24, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 24, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id BEFE21E8422; 24, 29 -- Fri, 6 Feb 2009 17:03:53 -0500 (EST) 24, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 24, 29 -- Fri, 6 Feb 2009 18:54:33 -0500 24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 29 -- Content-class: urn:content-classes:message 24, 29 -- MIME-Version: 1.0 24, 29 -- Content-Type: text/plain; 24, 29 -- charset="iso-8859-1" 24, 29 -- Subject: RE: [Microscopy] viaWWW: How to get rid of a magnetic field 24, 29 -- Date: Fri, 6 Feb 2009 18:53:37 -0500 24, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9DD9D3A-at-EXDRESBENMX012.numonyx.local} 24, 29 -- In-Reply-To: {200902051618.n15GIrfQ003072-at-ns.microscopy.com} 24, 29 -- X-MS-Has-Attach: 24, 29 -- X-MS-TNEF-Correlator: 24, 29 -- Thread-Topic: [Microscopy] viaWWW: How to get rid of a magnetic field 24, 29 -- Thread-Index: AcmHrZuX9Xe3jDGQQTSzo2BWfJRoQwBB0zAQ 24, 29 -- References: {200902051618.n15GIrfQ003072-at-ns.microscopy.com} 24, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 24, 29 -- To: {j.janssen-at-nki.nl} 24, 29 -- Cc: {Microscopy-at-Microscopy.com} 24, 29 -- X-OriginalArrivalTime: 06 Feb 2009 23:54:33.0786 (UTC) FILETIME=[427FB1A0:01C988B6] 24, 29 -- Content-Transfer-Encoding: 8bit 24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n16NsYBM006872 ==============================End of - Headers==============================
Hello to all, I have been through the Carbon Rod problems with an old Denton which only had a 10 Amp power supply. We ended up changing the power supply for a 20 Amp unit and using Carbon Rods from
Carbone of America 900 Harrison Street Bay City, MI 48708
Ask for SPK type carbon rods they are a little softer than some of the other types of compound rod.
Attention Jamie 989 894 2911 I found her to be very helpful and knowledgeable in solving this problem. My customer hasn't called me back complaining about poor coating or sputtering since the changes.
Good luck Peter Earl Electrovac Technologies
==============================Original Headers============================== 6, 27 -- From pwje-at-sympatico.ca Fri Feb 6 20:02:34 2009 6, 27 -- Received: from blu0-omc3-s6.blu0.hotmail.com (blu0-omc3-s6.blu0.hotmail.com [65.55.116.81]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1722XMu025315 6, 27 -- for {microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 20:02:34 -0600 6, 27 -- Received: from BLU0-SMTP75 ([65.55.116.74]) by blu0-omc3-s6.blu0.hotmail.com with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Fri, 6 Feb 2009 18:02:33 -0800 6, 27 -- X-Originating-IP: [76.65.17.200] 6, 27 -- X-Originating-Email: [pwje-at-sympatico.ca] 6, 27 -- Message-ID: {BLU0-SMTP75CEE35B43BEAF798EDFC6B9BE0-at-phx.gbl} 6, 27 -- Received: from peterf0x4h0jpn ([76.65.17.200]) by BLU0-SMTP75.blu0.hotmail.com over TLS secured channel with Microsoft SMTPSVC(6.0.3790.2668); 6, 27 -- Fri, 6 Feb 2009 18:02:32 -0800 6, 27 -- From: "Peter Earl" {pwje-at-sympatico.ca} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- Subject: Carbon Rods 6, 27 -- Date: Fri, 6 Feb 2009 21:02:32 -0500 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- format=flowed; 6, 27 -- charset="Windows-1252"; 6, 27 -- reply-type=original 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- X-Priority: 3 6, 27 -- X-MSMail-Priority: Normal 6, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 6, 27 -- Disposition-Notification-To: "Peter Earl" {pwje-at-sympatico.ca} 6, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 6, 27 -- X-OriginalArrivalTime: 07 Feb 2009 02:02:32.0538 (UTC) FILETIME=[236443A0:01C988C8] ==============================End of - Headers==============================
There exist some software packages that document every manipulation ever done on an image. In some contexts this might be useful for a lab or organization to implement. This is possible to do this with notebooks as well. In spite of any such systems I think it will always be possible for a worker to be intentionally deceptive with digital images like any other media.
Brightness, contrast and gamma have been listed as legitimate adjustments. Rossner and Yamada (The Journal of Cell Biology, Volume 166, Number 1, July 5, 2004 11–15; http://www.jcb.org/cgi/doi/10.1083/jcb.200406019 (an excellent reference paper for all persons starting with imaging work)) list gamma as altering the image in a way that requires disclosure. In the film and paper era we altered gamma freely, since films almost absolutely had gamma curves not equal to zero and by choice of paper you could compensate this characteristic to your taste and most people could tell if the print looked normal, or hard - with lost tones, but this was all considered fair play. Worse was the reciprocity failure of silver-based films, especially color ones, at exposures that might be used for fluorescence photos - at exposures greater than one second and worse for longer exposures. With reciprocity failure, 2 objects differing only slightly in brightness, would be greatly different in brightness in the captured image, thus automatically cleaning up slightly dimmer backgrounds.
The human eye has the capacity for tremendous dynamic range so it sees into the shadows. Most media used today to present images do not have more than 8-bit range, and monitors where people work with the images have no more typically. So a sample/specimen with details in the dark areas that the eye can adjust to separate, if recorded with even a 12-bit camera, the final image presented on the monitor or printed will have to be adjusted to lose some shades, or have the gamma adjusted to present brightnesses in a non-linear way to separate the darker areas.
At the bright end, the human eye is not so good and saturates at high intensities and can't separate close values well, where a CCD properly exposed can capture these values linearly and they can be displayed unaltered in contrast or gamma and make close, bright values discernable in a way that the eye has difficulty to perceive. So here it is not unethical to present what we CAN'T directly/easily observe.
Most people using a digital camera should know that almost no chip has a perfect pixel array, and most have maps stored to "correct defective pixels", and this may or may not be obvious or accessible to the user. This operation absolutely fabricates data to fill in the bad pixels. The Diagnostic Imaging Spot cameras I have used have a checkbox so that this can be disabled by choice; most people want a nice picture and leave it checked. Most people know not to make the essence of a publication about the value of one pixel.
What I tell people working at our facilities is that the captured and presented image should substantially represent what another researcher would see if they were to observe the same slide through the microscope; anything to change this simple rule should be explained in the presentation of the micrograph. If this is the case there will never be any error of falsification.
It really does, in the end, come down to the integrity of the researcher. Original raw data always needs to be saved. Manipulations need to be documented so the operations could be repeated exactly, if challenged, and the methods to create any image that would not be directly observable by another should disclosed to the public as processing since it does contribute to what is presented as "data".
Dale
colijn.1-at-osu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } While brightness, contrast and gamma are fine to adjust. I would not } feel comfortable in modifying the image to "cover up" a } blemish. Assuming you are using a rubber stamp tool or something } similar, you are modifying the information in the original } image. You are then changing the information content of the } picture. In my (not so humble) opinion, this is a dangerous step to take. } } Cheers, } Henk } } } } At 08:52 AM 02/06/09, you wrote: } } } } {...snip...} } } I don't think any reasonable person would chastise somebody's } } photographic evidence or scientific documentation because the } } contrast was altered or an errant blemish was covered up in the name } } of producing an eye-pleasing presentation. } } } } Stu Smalinskas, P.E. } } Metallurgist } } Plymouth, Michigan } } SKF USA } } {...snip...} } } } Hendrik O. Colijn colijn.1-at-osu.edu } Campus Electron Optics Facility Ohio State University } (614) 292-0674 http://www.ceof.ohio-state.edu } Time is that quality of nature which keeps events from happening all } at once. Lately it doesn't seem to be working. } } } ==============================Original Headers============================== } 12, 26 -- From colijn.1-at-osu.edu Fri Feb 6 09:47:49 2009 } 12, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) } 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n16FlnoU009705 } 12, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Feb 2009 09:47:49 -0600 } 12, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by } 12, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) } 12, 26 -- id {01N56C78QW7K8XXAO4-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; } 12, 26 -- Fri, 06 Feb 2009 10:47:48 -0500 (EST) } 12, 26 -- Received: from HOC1.ecr6.ohio-state.edu } 12, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu } 12, 26 -- (PMDF V6.3-x18 #31556) } 12, 26 -- with ESMTPA id {01N56C77W4LO8XWYNV-at-ecr6.ohio-state.edu} ; Fri, } 12, 26 -- 06 Feb 2009 10:47:47 -0500 (EST) } 12, 26 -- Date: Fri, 06 Feb 2009 10:48:34 -0500 } 12, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} } 12, 26 -- Subject: Re: [Microscopy] Re: Image Processing -- Ethics and Validity } 12, 26 -- In-reply-to: {200902061352.n16DqFVg007329-at-ns.microscopy.com} } 12, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu } 12, 26 -- To: smalinskas-at-yahoo.com } 12, 26 -- Cc: Microscopy-at-microscopy.com } 12, 26 -- Message-id: {01N56C77YSNY8XWYNV-at-ecr6.ohio-state.edu} } 12, 26 -- MIME-version: 1.0 } 12, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 12, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed } 12, 26 -- X-Env-From: auth/colijn.1-at-osu.edu } 12, 26 -- References: {200902061352.n16DqFVg007329-at-ns.microscopy.com} } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 20 -- From dac-at-research.umass.edu Sat Feb 7 07:02:28 2009 10, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n17D2QWe032390 10, 20 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 07:02:27 -0600 10, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged)) 10, 20 -- (authenticated bits=0) 10, 20 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n17D2NlT020950 10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 20 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 08:02:24 -0500 10, 20 -- Message-ID: {498D86A2.90006-at-research.umass.edu} 10, 20 -- Date: Sat, 07 Feb 2009 08:03:30 -0500 10, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 10, 20 -- MIME-Version: 1.0 10, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 10, 20 -- Subject: Re: [Microscopy] Image Processing -- Ethics and Validity 10, 20 -- References: {200902061551.n16FpwSc021122-at-ns.microscopy.com} 10, 20 -- In-Reply-To: {200902061551.n16FpwSc021122-at-ns.microscopy.com} 10, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Along this thread many years ago the Microscopy Society of America issued a position on image manipulation processing and storage. It is documented here:
"Ethical digital imaging requires that the original uncompressed image file be stored on archival media (e.g., CD-R) without any image manipulation or processing operation. All parameters of the production and acquisition of this file, as well as any subsequent processing steps, must be documented and reported to ensure reproducibility.
Generally, acceptable (non-reportable) imaging operations include gamma correction, histogram stretching, and brightness and contrast adjustments. All other operations (such as Unsharp-Masking, Gaussian Blur, etc.) must be directly identified by the author as part of the experimental methodology. However, for diffraction data or any other image data that is used for subsequent quantification, all imaging operations must be reported."
This policy was formulated by the Digital Image Processing & Ethics Group of the MSA Education Committee and was adopted as MSA policy at the Summer Council meeting August 2-3, 2003.
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 9, 11 -- From zaluzec-at-microscopy.com Sat Feb 7 15:34:10 2009 9, 11 -- Received: from [10.5.0.80] (msdvpn8.msd.anl.gov [130.202.238.72]) 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n17LY2dO001998 9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 7 Feb 2009 15:34:08 -0600 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p0624080ac5b3adc1d3a8-at-[10.5.0.80]} 9, 11 -- Date: Sun, 8 Feb 2009 08:03:59 +1030 9, 11 -- To: microscopy-at-microscopy.com 9, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 9, 11 -- Subject: MSA Policy on Digital Image Processing 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Question: Dear all, I have been the director of a new EM Core facility for just more than a year now. Besides dealing with the wide variety of samples and EM projects brought into our facility, I found myself confronted with a huge amount of image data, specimen blocks and grids, much more than I am used to. After keeping all the blocks and grids of each project for a year, we are running out of grid boxes and have not figured out a best storage and tracking system for grids. I know there are many EM facility directors regularly contribute to the List, I would like to find out what are the policies of other EM Core Facilities concerning keeping user/clientís embedded blocks, grids and image data? Do you keep all the blocks and grids after each project or do you give everything back to your clients after the completion of the project? If you keep the grids and blocks, how long do you keep them? What is the best way to store and index the grids from different projects? We find it easier to keep all the grids from one project in one grid box but that means we need more than 50 grid boxes a year. How about the images? Do you keep them all? Is there any particular software that you use to catalogue all the image files? Before I sign off, I would like to thank everyone in the list who generously shares his/her invaluable knowledge here. I was not trained as an electron microscopist, but was landed this job by a total twist of fate. The list and the archive have been a great resource for me. Thank you all. Sincerely, Ru-ching
I'm wading through my first semester of teaching EM to undergrads and am in need of some advice regarding preparing animal tissue for TEM. Given the set-up of my class (that was not designed by me, mind you), we are forced sometimes to stop part-way through a protocol and store the tissue for a day or so. In my experience with plant tissue, I've done this at various steps, however, I need your advice on stopping points for animal tissue. Any advice on when tissue can be stored (and when it cannot), would be much appreciated. For reference, we are doing a "standard" primary fixation in glut/phosphate buffer, secondary in OsO4/phosphate buffer, dehydration in either EtOH or acetone and embedding in Spurr's.
Thanks in advance for sharing your wisdom, Kristen Lennon
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology 202 Compton Science Center Frostburg State University 101 Braddock Road Frostburg, MD 21532
301-687-4697 k.lennon-at-frostburg.edu
==============================Original Headers============================== 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009 7, 20 -- Received: from web84004.mail.mud.yahoo.com (web84004.mail.mud.yahoo.com [68.142.206.174]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n18Mi9uL003740 7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 16:44:09 -0600 7, 20 -- Received: (qmail 89133 invoked by uid 60001); 8 Feb 2009 22:44:08 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 20 -- b=Q/nvtsNH4vIclnMSFk8HKzn89tSpHP+uPS3v9pqayDcp7e2unovlolRJ38hLGqSGzMjqonKNWCCWqdLQUlFfVfWe+WvX0p0bLd7yHYyyFmYwHVBJ0gHFuyDwdoAMhMCOcXkAycrFx7xVC8x5vFUuGz04l5C+TdSbza5Vd8gWep0=; 7, 20 -- X-YMail-OSG: Mrb7abkVM1nbSZ1kLFQvRXfaSKhXrYcO2H9I29ijeTEs9Kd9MrICv3AnvJxZhZ7f3TFi337dCvHBJEt7pKZpBg4Sk2pQMakt8kn.Ov5UewnFkrpuISmbycMqL2PoTJdK007lQ4yloiuC_z0OQvclV6CHeZOPiJ06Y17O3JwR7pjarx560Pj94rLDXCdlqvKS_4I6Ho5K3WVkuwXJA6SRWqAFwIcHyUrRUQ-- 7, 20 -- Received: from [96.239.128.209] by web84004.mail.mud.yahoo.com via HTTP; Sun, 08 Feb 2009 14:44:08 PST 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 7, 20 -- Date: Sun, 8 Feb 2009 14:44:08 -0800 (PST) 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} 7, 20 -- Reply-To: kamlennon-at-yahoo.com 7, 20 -- Subject: teaching: stopping points for preparing tissue? 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Message-ID: {558194.87385.qm-at-web84004.mail.mud.yahoo.com} ==============================End of - Headers==============================
I have had best luck stopping at 70% EtOH. It is the only stopping point where I don't see problems. That said, my samples are not your samples..... David
On Feb 8, 2009, at 3:47 PM, kamlennon-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Listers, } } I'm wading through my first semester of teaching EM to undergrads } and am in need of some advice regarding preparing animal tissue for } TEM. Given the set-up of my class (that was not designed by me, } mind you), we are forced sometimes to stop part-way through a } protocol and store the tissue for a day or so. In my experience } with plant tissue, I've done this at various steps, however, I need } your advice on stopping points for animal tissue. Any advice on } when tissue can be stored (and when it cannot), would be much } appreciated. For reference, we are doing a "standard" primary } fixation in glut/phosphate buffer, secondary in OsO4/phosphate } buffer, dehydration in either EtOH or acetone and embedding in } Spurr's. } } Thanks in advance for sharing your wisdom, } Kristen Lennon } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } } 301-687-4697 } k.lennon-at-frostburg.edu } } } } } } ==============================Original } Headers============================== } 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009 } 7, 20 -- Received: from web84004.mail.mud.yahoo.com } (web84004.mail.mud.yahoo.com [68.142.206.174]) } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id n18Mi9uL003740 } 7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 16:44:09 } -0600 } 7, 20 -- Received: (qmail 89133 invoked by uid 60001); 8 Feb 2009 } 22:44:08 -0000 } 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 7, 20 -- s=s1024; d=yahoo.com; } 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply- } To:Subject:To:MIME-Version:Content-Type:Message-ID; } 7, 20 -- b=Q/nvtsNH4vIclnMSFk8HKzn89tSpHP } +uPS3v9pqayDcp7e2unovlolRJ38hLGqSGzMjqonKNWCCWqdLQUlFfVfWe } +WvX0p0bLd7yHYyyFmYwHVBJ0gHFuyDwdoAMhMCOcXkAycrFx7xVC8x5vFUuGz04l5C } +TdSbza5Vd8gWep0=; } 7, 20 -- X-YMail-OSG: } Mrb7abkVM1nbSZ1kLFQvRXfaSKhXrYcO2H9I29ijeTEs9Kd9MrICv3AnvJxZhZ7f3TFi33 } 7dCvHBJEt7pKZpBg4Sk2pQMakt8kn.Ov5UewnFkrpuISmbycMqL2PoTJdK007lQ4yloiuC } _z0OQvclV6CHeZOPiJ06Y17O3JwR7pjarx560Pj94rLDXCdlqvKS_4I6Ho5K3WVkuwXJA6 } SRWqAFwIcHyUrRUQ-- } 7, 20 -- Received: from [96.239.128.209] by } web84004.mail.mud.yahoo.com via HTTP; Sun, 08 Feb 2009 14:44:08 PST } 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 } 7, 20 -- Date: Sun, 8 Feb 2009 14:44:08 -0800 (PST) } 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } 7, 20 -- Reply-To: kamlennon-at-yahoo.com } 7, 20 -- Subject: teaching: stopping points for preparing tissue? } 7, 20 -- To: microscopy-at-microscopy.com } 7, 20 -- MIME-Version: 1.0 } 7, 20 -- Content-Type: text/plain; charset=us-ascii } 7, 20 -- Message-ID: {558194.87385.qm-at-web84004.mail.mud.yahoo.com} } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Sun Feb 8 18:05:33 2009 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1905X2x019149 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 18:05:33 -0600 5, 22 -- Received: from gandalfs_amavis (amavis10.email.arizona.edu [10.0.0.238]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 18A819B97D0 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:33 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7513E9B97B9 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:32 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 5, 22 -- In-Reply-To: {200902082247.n18MlA4R008086-at-ns.microscopy.com} 5, 22 -- References: {200902082247.n18MlA4R008086-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {A8761809-081C-4B60-A8A9-24CFE82D3397-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] teaching: stopping points for preparing tissue? 5, 22 -- Date: Sun, 8 Feb 2009 17:05:30 -0700 5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com} 5, 22 -- X-Mailer: Apple Mail (2.753.1) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Our SEM studies of hair are purely morphological. We look for things like twists, kinks, knots, longitudinal impressions, or even invaginations of the hair shaft (so-called "bamboo hair"). We also look at the cuticle for signs of loss or fracture. Hair shaft abnormalities may be associated with skin disorders or inborn errors of metabolism. Bear in mind that exogenous factors can also affect hair morphology.
We also look at hair using polarised light which may produce lovely banding patterns, eg, the disease called trichothiodystrophy produces alternating light and dark bands under polarised light (so-called "tiger-tail" pattern). It's thought to be the result of low sulphur content within the hair.
Regards,
John
Hi John,
I am curious about the baby hair you look at. Are you looking for morphological abnormalities, elemental composition, or both? I have never heard of this before.
Thanks, Andrea
Andrea Blake Brothers Senior Scientist, MMCC Microscopy & Microanalysis Characterization Center andrea.brothers-at-biovail.com
(703) 480-5879 office (703) 480-5943 fax
BIOVAIL 3701 Concorde Parkway Chantilly, VA 20151
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-----Original Message----- X-from: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au] Sent: Thursday, February 05, 2009 6:01 PM To: Andrea Brothers
Hi Jon,
We have an old Hitachi S-520 SEM still operational. Once or twice a year we are asked to look at paediatric hair specimens for defects. Of course, babies can't tell what's wrong with them so looking at hair samples can sometimes give you a clue as to what is the problem. Ie, the hair defect may be one part of a wider syndrome.
Regards,
John Brealey
Senior Medical Scientist, Electron Microscopy Unit
T 08 8222 6612 F 08 8222 6425
www.sapathology.sa.gov.au
SA Pathology (TQEH) Quality Pathology supporting Training and Research
==============================Original Headers============================== 29, 27 -- From john.brealey-at-imvs.sa.gov.au Sun Feb 8 23:12:42 2009 29, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 29, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n195CdcQ013965 29, 27 -- for {Microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 23:12:41 -0600 29, 27 -- X-IronPort-AV: E=Sophos;i="4.37,403,1231075800"; 29, 27 -- d="scan'208";a="9491193" 29, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 29, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 09 Feb 2009 15:42:36 +1030 29, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 29, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 29, 27 -- 8.1.263.0; Mon, 9 Feb 2009 15:41:45 +1030 29, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au 29, 27 -- (Postfix) with ESMTP id 6FE2734BA4 for {Microscopy-at-microscopy.com} ; Mon, 9 29, 27 -- Feb 2009 15:42:36 +1030 (CST) 29, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 29, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 29, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 29, 27 -- Subject: SEM of Hair 29, 27 -- Date: Mon, 9 Feb 2009 15:42:35 +1030 29, 27 -- Organization: IMVS 29, 27 -- Message-ID: {000001c98a75$05166410$5954150a-at-41347i} 29, 27 -- MIME-Version: 1.0 29, 27 -- Content-Type: text/plain; charset="us-ascii" 29, 27 -- Content-Transfer-Encoding: 7bit 29, 27 -- X-Mailer: Microsoft Office Outlook 11 29, 27 -- Thread-Index: AcmKdQTt+EepJkF0Qx23XNV1d8kjjw== 29, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
The point after GA fixation and 2-3 rinses is a good early point. After OsO4 and rinses is not bad too. Lower concentration alcohols is a big NO. 70% is OK to leave overnight in the fridge and convenient to combine with UA staining, making it 1.5% UA in 70% ethanol.
In general, any excessive time in alcohol or acetone causes extraction, so samples should not be left there for days. Same for diluted epoxy. Acetone has been reported to be less extractive during dehydration than ethanol, but it is harder to handle.
There is one more point worth mentioning. I've never seen it described anywhere, but once had to fix an important sample on the eve of vacation. I reasoned that undiluted ethanol in the freezer (-20C) should minimize any extraction and will also not protect from freezing damage. A week later (yes, US vacations...) I embedded the samples, and all was perfect.
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
} Hi Listers, } } I'm wading through my first semester of teaching EM to undergrads } and am in need of some advice regarding preparing animal tissue for } TEM. Given the set-up of my class (that was not designed by me, mind } you), we are forced sometimes to stop part-way through a protocol } and store the tissue for a day or so. In my experience with plant } tissue, I've done this at various steps, however, I need your advice } on stopping points for animal tissue. Any advice on when tissue can } be stored (and when it cannot), would be much appreciated. For } reference, we are doing a "standard" primary fixation in glut/ } phosphate buffer, secondary in OsO4/phosphate buffer, dehydration in } either EtOH or acetone and embedding in Spurr's. } } Thanks in advance for sharing your wisdom, } Kristen Lennon } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } 202 Compton Science Center } Frostburg State University } 101 Braddock Road } Frostburg, MD 21532 } } 301-687-4697 } k.lennon-at-frostburg.edu } } } } } } ==============================Original } Headers============================== } 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009 } 7, 20 -- Received: from web84004.mail.mud.yahoo.com } (web84004.mail.mud.yahoo.com [68.142.206.174]) } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
If there are no wonders, there are nevertheless some interesting (and simple) idees to follow up.
Jacques
-------- Message original -------- Sujet: [Microscopy] SEM holder for TEM grids
Hi all
As there is again some demand here for observations of TEM grids by SEM, I took out of the drawer the home made TEM grids holders I had machined a while ago. And non of them is really practical to use. The first was made with an EM300 tip, and works right, but it's a one shot and for limited to the jeol 840 serie stage. The second is delicate to use, and I fear to bent the grids each time I take them away from the holder.
So what kind of holder do other use for TEM grids (only SEM observations, no STEM), which allows very short WD and an easy way to mount/umount 5-6 grids in a batch ? I've soon looked in some catalogues, but certainly not at all sources. And users advices are very usfull !
I'm interested in any ideas and/or squetches for home manufacturing in our workshop, and/or for documentions, users advices and manufacturer doc and quoting etc.
Thanks in advance, and have a good WE !
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
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-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
I would strongly recommend having your users take and store their own samples themselves, and the earlier you start having them do it, the better.
For years, we had been keeping samples in storage for our clients, until the volume became overwhelming. Samples, grids,blocks, etc. were piled everywhere. We are now in the very slow process of getting the samples back to those researchers who still want them. The problem is that in some cases, especially high-volume users, the resistance to taking their own samples to their own labs is, well, pretty intense. They got too used to having us do it for them, but then what happens is they might come in and want to review a project from years ago. If we can't find the exact set of specimens, blocks, or grids on short notice, it can get dicey.
We are considering instituting a storage fee to provide a little nudge to get people to take their own stuff back. Fees are very effective behavior modifiers and can be used not only for this, but for discouraging late-shows or no-shows on scopes, messes being left behind by sloppy users, failure to fill out forms and logbooks, etc. We generally find we only have to charge a behavior-mod fee (BMF) once or twice and that behavior has been modded.
Regarding images, we generally archive them here in digital form, which is not a huge problem with large, cheap hard-drives readily available. We used to back up images on CD's and DVD's, but now we generally leave on big hard drives backing up our primary hard drives on the scope computers. That said, we have very rarely been asked to dig up old images, so it may not really be necessary. It would not be unreasonable, in my opinion, to make the user responsible for this aspect of their work, too.
Every lab is different and we all operate under different sets of instructions, but this is how we go about things in our facility.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: rhsia-at-umaryland.edu [mailto:rhsia-at-umaryland.edu] Sent: Sunday, February 08, 2009 3:02 PM To: Tindall, Randy D.
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Question: Dear all, I have been the director of a new EM Core facility for just more than a year now. Besides dealing with the wide variety of samples and EM projects brought into our facility, I found myself confronted with a huge amount of image data, specimen blocks and grids, much more than I am used to. After keeping all the blocks and grids of each project for a year, we are running out of grid boxes and have not figured out a best storage and tracking system for grids. I know there are many EM facility directors regularly contribute to the List, I would like to find out what are the policies of other EM Core Facilities concerning keeping user/clientís embedded blocks, grids and image data? Do you keep all the blocks and grids after each project or do you give everything back to your clients after the completion of the project? If you keep the grids and blocks, how long do you keep them? What is the best way to store and index the grids from different projects? We find it easier to keep all the grids from one project in one grid box but that means we need more than 50 grid boxes a year. How about the images? Do you keep them all? Is there any particular software that you use to catalogue all the image files? Before I sign off, I would like to thank everyone in the list who generously shares his/her invaluable knowledge here. I was not trained as an electron microscopist, but was landed this job by a total twist of fate. The list and the archive have been a great resource for me. Thank you all. Sincerely, Ru-ching
Dear Ru-ching- I've run a core facility for 20 years and I have almost every sample I've ever prepared...blocks and grids. The exceptions have been when investigators have left this institution and were planning on continuing their work at their new home. I have given them their materials and noted the transfer in my records. My record system is a little redundant because it has, over the years, gone from paper only, to Excel to our on-line information management system (LIMS). Briefly: Each request that comes in is logged. Our log numbers consist of the last 2-digits of the current calendar year, followed by next number in sequence eg: 09-003 is followed by 09-004, etc.. This number is entered on the protocol sheet that is created for each request, as well as on a "Log'in" sheet that is kept on a clip board. Also noted are the date, requester's name, head of lab, type of sample (TEM, SEM, paraffin, cryo, etc). There are columns for checking off when the work is completed and when it has been billed. We prepare a protocol sheet for each request on which we detail how the sample(s) has been handled. Here's the redundancy: the samples are logged into our LIMS, and I still enter it into the Excel sheet, because it is the easiest to search. As far as storage: Now that everyone is going to digital imaging I will have to come up with a new system. For years, I have stored my old grid boxes and the white slider "match boxes" in which I store my blocks, in the 500-sheet boxes from 8x10 photographic paper. They work out to hold about 3-4 years worth of grid boxes (of the style I use) and about 2 years worth of slide boxes. Those are stashed on the top shelves of the wall cabinets in my lab. (since I am a bit "vertically challenged" I don't keep anything I need ready-access to that high up!). So yes, I have hundreds of grid boxes sitting in storage, with samples going back to my predecessor (back to 1982). the boxes are labelled by month and year. I can always find old grids when they are requested. I have always given the negatives (& in the old days, prints) back to the investigator. I now keep copies of the scanned negatives or digital images on a hard drive in the lab. The investigator gets them on CD. If you'd like more details, just write. Good luck, Lee } --------------------------------------------------------------------------- } } Email: rhsia-at-umaryland.edu } Name: Ru-ching Hsia } } Organization: U Maryland } } Title-Subject: [Filtered] Core Facility data/sample storage } } Question: Dear all, } I have been the director of a new EM Core } facility for just more than a year now. Besides } dealing with the wide variety of samples and EM } projects brought into our facility, I found } myself confronted with a huge amount of image } data, specimen blocks and grids, much more than } I am used to. After keeping all the blocks and } grids of each project for a year, we are running } out of grid boxes and have not figured out a best } storage and tracking system for grids. I know } there are many EM facility directors regularly } contribute to the List, I would like to find out } what are the policies of other EM Core Facilities } concerning keeping user/clientís embedded blocks, } grids and image data? } Do you keep all the blocks and grids after each } project or do you give everything back to your } clients after the completion of the project? } If you keep the grids and blocks, how long do you } keep them? What is the best way to store and } index the grids from different projects? We find } it easier to keep all the grids from one project } in one grid box but that means we need more than } 50 grid boxes a year. } How about the images? Do you keep them all? Is } there any particular software that you use to } catalogue all the image files? } Before I sign off, I would like to thank everyone } in the list who generously shares his/her } invaluable knowledge here. I was not trained as } an electron microscopist, but was landed this job } by a total twist of fate. The list and the } archive have been a great resource for me. Thank } you all. } Sincerely, } Ru-ching
-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
I agree totally with Randy. Once the project has been completed we expect the users to take and store their own specimens. We also expect all users to archive and store their own data. We started, 11 years ago, by also storing them in digital form before taking them off instrument computers, but after a few years the number of CD-R disks was becoming impossible. There were only a very few occasions that users asked for the archived images so we no longer do it.
Regards
Alan
At 08:35 AM 2/9/2009, TindallR-at-missouri.edu wrote:
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Alan W Nicholls, PhD Interim Associate Director - RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
As John Bozzola mentioned, there can be many reasons for a blank negative, but one very valuable tool in diagnosing the cause of imaging problems on negatives is the scope data that the instrument itself flashes onto the negative. This would be the mag marker, kV information, magnification, or whatever your particular microscope records on the film.
For example, if the entire negative is blank, including the scope information, then either the scope is completely failing to record anything, the film was loaded emulsion side down, your chemicals are bad, or you accidentally put the film in fixer before the developer (been there, done that).
If the scope information looks normal, but the sample image is missing or really light, then the problem is with the recording of the sample in the scope, not with the developing side of things. Your exposure time may be too brief or the sample exposure isn't happening at all.
If both the scope data and sample image are very light, then the problem is almost always exhausted chemicals, overly diluted developer, very cold developer, or developing times that are too short. Sometimes this can also indicate improper exposure settings for the microscope, but I think the mag marker, etc., exposure is generally set at the factory and checked by the service engineers during set-up. I have personally never seen it go wrong. Another possible cause is way outdated film, but this would normally be accompanied by an overall gray fog and lousy contrast.
Hope some of this is useful.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Friday, February 06, 2009 3:57 PM To: Tindall, Randy D.
} } I just changed the filament for Philips EM300 and saturated, the } illumination looks good and I observe the samples. But the problem is } when I develop the negatives, it looks empty. There was no image, } Please let me know what is the problem and why the film is empty. } Thanks for your help.
=============
==============================Original Headers============================== 18, 29 -- From TindallR-at-missouri.edu Mon Feb 9 09:32:15 2009 18, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19FWD3Q017928 18, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:32:14 -0600 18, 29 -- X-IronPort-Anti-Spam-Filtered: true 18, 29 -- X-IronPort-Anti-Spam-Result: ApoEAIvaj0nRauUo/2dsb2JhbADCfgEJhBSISoJYC4E3Bg 18, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 18, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Feb 2009 09:32:10 -0600 18, 29 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 18, 29 -- Mon, 9 Feb 2009 09:32:10 -0600 18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 29 -- Content-class: urn:content-classes:message 18, 29 -- MIME-Version: 1.0 18, 29 -- Content-Type: text/plain; 18, 29 -- charset="us-ascii" 18, 29 -- Subject: RE: [Microscopy] RE: Philips EM300 plate film: no image on negatives 18, 29 -- Date: Mon, 9 Feb 2009 09:32:10 -0600 18, 29 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DA8-at-UM-XMAIL08.um.umsystem.edu} 18, 29 -- In-Reply-To: {200902062156.n16LulTb014586-at-ns.microscopy.com} 18, 29 -- X-MS-Has-Attach: 18, 29 -- X-MS-TNEF-Correlator: 18, 29 -- Thread-Topic: [Microscopy] RE: Philips EM300 plate film: no image on negatives 18, 29 -- Thread-Index: AcmIpc9Jb7kqJsWoTCiTowXM0NJ9zACIwEgA 18, 29 -- References: {200902062156.n16LulTb014586-at-ns.microscopy.com} 18, 29 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 18, 29 -- To: {microscopy-at-microscopy.com} 18, 29 -- X-OriginalArrivalTime: 09 Feb 2009 15:32:10.0904 (UTC) FILETIME=[932E2980:01C98ACB] 18, 29 -- Content-Transfer-Encoding: 8bit 18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19FWD3Q017928 ==============================End of - Headers==============================
Hi, I would like to thank every one for your valuable suggestions. I tried all of them and find the following results.
As I told, I Lift the large screen and without transporting a film cassette I pressed the exposure button.
I did not see an image on the small screen and I did not even see light beam also. So please let me know if any one know the solution for this problem or should I need service engineer to do this.
Sincerely Kabilan UMDNJ-NJDS Newark, NJ, USA
==============================Original Headers============================== 5, 26 -- From velliyka-at-umdnj.edu Mon Feb 9 09:33:52 2009 5, 26 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n19FXojn019721 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:33:51 -0600 5, 26 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 5, 26 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 7E07E4C087 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:33:46 -0500 (EST) 5, 26 -- Received: from umdnj.edu (unknown [10.32.15.102]) 5, 26 -- by zix04.umdnj.edu (Proprietary) with ESMTP id BECD650C023 5, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:32:05 -0500 (EST) 5, 26 -- Received: from ([10.32.15.171]) 5, 26 -- by imail2.umdnj.edu with ESMTP id CVSJWG1.6716359; 5, 26 -- Mon, 09 Feb 2009 10:27:25 -0500 5, 26 -- MIME-version: 1.0 5, 26 -- Content-transfer-encoding: 7BIT 5, 26 -- Content-type: text/plain; charset=US-ASCII; format=flowed 5, 26 -- Received: from [10.4.62.229] ([10.32.15.102]) 5, 26 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built 5, 26 -- Mar 14 2008; 32bit)) with ESMTP id {0KET00BY41LPNSA0-at-umduwc02.umdnj.edu} for 5, 26 -- Microscopy-at-microscopy.com; Mon, 09 Feb 2009 10:27:25 -0500 (EST) 5, 26 -- Message-id: {1fac03542de90fc883d302d713f7ebe0-at-umdnj.edu} 5, 26 -- To: Microscopy-at-microscopy.com 5, 26 -- From: Kabilan Velliyagounder {velliyka-at-umdnj.edu} 5, 26 -- Subject: RE: Philips EM300 plate film: no image on negatives 5, 26 -- Date: Sun, 08 Feb 2009 22:30:38 -0500 5, 26 -- X-Mailer: Apple Mail (2.624) ==============================End of - Headers==============================
You do not say if the problem is ac field or a dc field. The existing Faraday cage would help attenuate constant dc fields and would also significantly reduce high frequency ac fields but would probably not significantly attenuate 50/60Hz fields.
The chances are that if it is due to the trams it is a varying dc field that occurs as each tram enters the section and starts to draw power. Field cancelation systems work best when the field to be cancelled does not vary, that is it is always the same frequency with always the same amplitude and shape - we have an IDE Helmholtz coil system that allowed our field emission TEM/STEM to be used while facilities spent several months looking for the source of a 40mG pure, constant 60Hz field which suddenly appeared one winter! This system cost ~$40K ten years ago.
In a previous life I was involved with a dedicated STEM being installed in the former East Germany. The lab there had tram tracks on one side of the building with a power feeder for the trams running along another wall. In this case the installation engineer used a Hall effect probe to detect the dc field shift and fed the opposite offset into a set of alignment coils. This worked and stopped the image annoyingly jumping side to side as the trams entered the section. The cost in this case was a few dollars in parts and a few hours of labor!
The instrument you want to move is a Tecnai12? If this is used for life science work at relatively low magnifications ( {100K) then the effect of the field may not be noticeable. The microscope would not meet performance specifications but you would not be using it anywhere near that limit. This would not be ideal, but may be cheaper!
In general the best approach to fields, wether dc or ac is to avoid them!
Good luck
Alan
} } -----Original Message----- } X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl] } Sent: Thursday, February 05, 2009 8:19 AM } Subject: [Microscopy] viaWWW: How to get rid of a magnetic field } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Alan W Nicholls, PhD Interim Associate Director - RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Buffer after glutaraldehyde or buffer after osmium are the best places to stop and store tissue. Storage in any concentration of alcohol (or acetone or prop.oxide) is NOT a good idea, cytoplasm will be extracted. This is well documented in the literature. If you can get to pure resin put the vials in the refrigerator overnight. Just remember to let things warm to room temp. before opening to avoid condensation.
Geoff
Elliott-at-arizona.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have had best luck stopping at 70% EtOH. It is the only stopping } point where I don't see problems. That said, my samples are not your } samples..... } David } } } On Feb 8, 2009, at 3:47 PM, kamlennon-at-yahoo.com wrote: } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Hi Listers, } } } } I'm wading through my first semester of teaching EM to undergrads } } and am in need of some advice regarding preparing animal tissue for } } TEM. Given the set-up of my class (that was not designed by me, } } mind you), we are forced sometimes to stop part-way through a } } protocol and store the tissue for a day or so. In my experience } } with plant tissue, I've done this at various steps, however, I need } } your advice on stopping points for animal tissue. Any advice on } } when tissue can be stored (and when it cannot), would be much } } appreciated. For reference, we are doing a "standard" primary } } fixation in glut/phosphate buffer, secondary in OsO4/phosphate } } buffer, dehydration in either EtOH or acetone and embedding in } } Spurr's. } } } } Thanks in advance for sharing your wisdom, } } Kristen Lennon } } } } Kristen A. Lennon, Ph.D. } } Lecturer, Department of Biology } } 202 Compton Science Center } } Frostburg State University } } 101 Braddock Road } } Frostburg, MD 21532 } } } } 301-687-4697 } } k.lennon-at-frostburg.edu } } } } } } } } } } } } ==============================Original } } Headers============================== } } 7, 20 -- From kamlennon-at-yahoo.com Sun Feb 8 16:44:09 2009 } } 7, 20 -- Received: from web84004.mail.mud.yahoo.com } } (web84004.mail.mud.yahoo.com [68.142.206.174]) } } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } id n18Mi9uL003740 } } 7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 16:44:09 } } -0600 } } 7, 20 -- Received: (qmail 89133 invoked by uid 60001); 8 Feb 2009 } } 22:44:08 -0000 } } 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 7, 20 -- s=s1024; d=yahoo.com; } } 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply- } } To:Subject:To:MIME-Version:Content-Type:Message-ID; } } 7, 20 -- b=Q/nvtsNH4vIclnMSFk8HKzn89tSpHP } } +uPS3v9pqayDcp7e2unovlolRJ38hLGqSGzMjqonKNWCCWqdLQUlFfVfWe } } +WvX0p0bLd7yHYyyFmYwHVBJ0gHFuyDwdoAMhMCOcXkAycrFx7xVC8x5vFUuGz04l5C } } +TdSbza5Vd8gWep0=; } } 7, 20 -- X-YMail-OSG: } } Mrb7abkVM1nbSZ1kLFQvRXfaSKhXrYcO2H9I29ijeTEs9Kd9MrICv3AnvJxZhZ7f3TFi33 } } 7dCvHBJEt7pKZpBg4Sk2pQMakt8kn.Ov5UewnFkrpuISmbycMqL2PoTJdK007lQ4yloiuC } } _z0OQvclV6CHeZOPiJ06Y17O3JwR7pjarx560Pj94rLDXCdlqvKS_4I6Ho5K3WVkuwXJA6 } } SRWqAFwIcHyUrRUQ-- } } 7, 20 -- Received: from [96.239.128.209] by } } web84004.mail.mud.yahoo.com via HTTP; Sun, 08 Feb 2009 14:44:08 PST } } 7, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 } } 7, 20 -- Date: Sun, 8 Feb 2009 14:44:08 -0800 (PST) } } 7, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } } 7, 20 -- Reply-To: kamlennon-at-yahoo.com } } 7, 20 -- Subject: teaching: stopping points for preparing tissue? } } 7, 20 -- To: microscopy-at-microscopy.com } } 7, 20 -- MIME-Version: 1.0 } } 7, 20 -- Content-Type: text/plain; charset=us-ascii } } 7, 20 -- Message-ID: {558194.87385.qm-at-web84004.mail.mud.yahoo.com} } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original Headers============================== } 5, 22 -- From Elliott-at-arizona.edu Sun Feb 8 18:05:33 2009 } 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1905X2x019149 } 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 18:05:33 -0600 } 5, 22 -- Received: from gandalfs_amavis (amavis10.email.arizona.edu [10.0.0.238]) } 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 18A819B97D0 } 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:33 -0700 (MST) } 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7513E9B97B9 } 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 8 Feb 2009 17:05:32 -0700 (MST) } 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) } 5, 22 -- In-Reply-To: {200902082247.n18MlA4R008086-at-ns.microscopy.com} } 5, 22 -- References: {200902082247.n18MlA4R008086-at-ns.microscopy.com} } 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 5, 22 -- Message-Id: {A8761809-081C-4B60-A8A9-24CFE82D3397-at-arizona.edu} } 5, 22 -- Content-Transfer-Encoding: 7bit } 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} } 5, 22 -- Subject: Re: [Microscopy] teaching: stopping points for preparing tissue? } 5, 22 -- Date: Sun, 8 Feb 2009 17:05:30 -0700 } 5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com} } 5, 22 -- X-Mailer: Apple Mail (2.753.1) } 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 7, 28 -- From mcauliff-at-umdnj.edu Mon Feb 9 09:56:18 2009 7, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n19FuGMx027287 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 09:56:17 -0600 7, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 7, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 8EDA2A7B5B 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:56:15 -0500 (EST) 7, 28 -- Received: from umdnj.edu (unknown [10.32.15.102]) 7, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 1FD61A7B6B 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:53:55 -0500 (EST) 7, 28 -- Received: from ([10.32.15.171]) 7, 28 -- by imail.umdnj.edu with ESMTP id 8XSJWG1.6442831; 7, 28 -- Mon, 09 Feb 2009 10:49:24 -0500 7, 28 -- MIME-version: 1.0 7, 28 -- Content-transfer-encoding: 7BIT 7, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 7, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 7, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-6.03 (built 7, 28 -- Mar 14 2008; 32bit)) with ESMTP id {0KET00BCN2MCNSD0-at-umduwc02.umdnj.edu} for 7, 28 -- microscopy-at-microscopy.com; Mon, 09 Feb 2009 10:49:24 -0500 (EST) 7, 28 -- Message-id: {499050D4.2010900-at-umdnj.edu} 7, 28 -- Date: Mon, 09 Feb 2009 10:50:44 -0500 7, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 7, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 7, 28 -- To: Elliott-at-arizona.edu, microscopy-at-microscopy.com 7, 28 -- Subject: Re: [Microscopy] Re: teaching: stopping points for preparing tissue? 7, 28 -- References: {200902090006.n1906fhk020682-at-ns.microscopy.com} 7, 28 -- In-reply-to: {200902090006.n1906fhk020682-at-ns.microscopy.com} ==============================End of - Headers==============================
Alan; Did they find the source of the 40mG field? Can you reveal what it was and the why and how of how it suddenly appeared?
John Mardinly, Numonyx
-----Original Message----- X-from: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu] Sent: Monday, February 09, 2009 7:51 AM To: MARDINLY, A
Hans
You do not say if the problem is ac field or a dc field. The existing Faraday cage would help attenuate constant dc fields and would also significantly reduce high frequency ac fields but would probably not significantly attenuate 50/60Hz fields.
The chances are that if it is due to the trams it is a varying dc field that occurs as each tram enters the section and starts to draw power. Field cancelation systems work best when the field to be cancelled does not vary, that is it is always the same frequency with always the same amplitude and shape - we have an IDE Helmholtz coil system that allowed our field emission TEM/STEM to be used while facilities spent several months looking for the source of a 40mG pure, constant 60Hz field which suddenly appeared one winter! This system cost ~$40K ten years ago.
In a previous life I was involved with a dedicated STEM being installed in the former East Germany. The lab there had tram tracks on one side of the building with a power feeder for the trams running along another wall. In this case the installation engineer used a Hall effect probe to detect the dc field shift and fed the opposite offset into a set of alignment coils. This worked and stopped the image annoyingly jumping side to side as the trams entered the section. The cost in this case was a few dollars in parts and a few hours of labor!
The instrument you want to move is a Tecnai12? If this is used for life science work at relatively low magnifications ( {100K) then the effect of the field may not be noticeable. The microscope would not meet performance specifications but you would not be using it anywhere near that limit. This would not be ideal, but may be cheaper!
In general the best approach to fields, wether dc or ac is to avoid them!
Good luck
Alan
} } -----Original Message----- } X-from: j.janssen-at-nki.nl [mailto:j.janssen-at-nki.nl] } Sent: Thursday, February 05, 2009 8:19 AM } Subject: [Microscopy] viaWWW: How to get rid of a magnetic field } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Alan W Nicholls, PhD Interim Associate Director - RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
It's beginning to sound as if the mechanical shutter which closes, opens, then closes again for the exposure has failed. It's a long time since I've used a 300 but I'm sure I had a similar problem with an even older AEI 801 and if I remember rightly a lead had become dislodged from the vicinity of the shutter mechanism at the back of the microscope column.
A lot of the older shutters have a characteristic noise as the mechanism works, this may give a clue if it's stopped happening.
If you have an image that is visible before photography and nothing happens during the exposure time, then the only other thing worth trying is perhaps experimenting with exposure times to see if it's just one setting that has failed.
You may well need to contact an engineer so unless you already know one who services Philips EM300s, this might be a good time to find someone because you may need him/her again.
Good luck
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences Fleming Building University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: velliyka-at-umdnj.edu
I agree with giving sample blocks, grids, data, etc to the investigators immediately after completing the project. We cannot/should not use them and we should not be responsible for their safe keeping. That is the responsibility of the investigators.
We do backup service project digital images and hold them for a few months. Then I will write DVDs and store those. We also backup independent user digital images for a while but periodically ask them to clear all images off our computers. They are told that they should copy images onto portable media immediately after each session. We hold no responsibility for loss if a hard drive fails, etc and will not go to extraordinary effort to reclaim lost data.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: Alan Nicholls {nicholls-at-uic.edu} } Reply-To: Alan Nicholls {nicholls-at-uic.edu} } Date: Mon, 9 Feb 2009 09:13:26 -0600 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] viaWWW: Core Facility data/sample storage } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ru-ching } } I agree totally with Randy. Once the project has been completed we expect } the users to take and store their own specimens. We also expect all users } to archive and store their own data. We started, 11 years ago, by also } storing them in digital form before taking them off instrument computers, } but after a few years the number of CD-R disks was becoming impossible. } There were only a very few occasions that users asked for the archived } images so we no longer do it. } } Regards } } Alan } } At 08:35 AM 2/9/2009, TindallR-at-missouri.edu wrote: } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Ru-ching, } } } } I would strongly recommend having your users take and store their own } } samples themselves, and the earlier you start having them do it, the better. } } } } For years, we had been keeping samples in storage for our clients, until } } the volume became overwhelming. Samples, grids,blocks, etc. were piled } } everywhere. We are now in the very slow process of getting the samples } } back to those researchers who still want them. The problem is that in } } some cases, especially high-volume users, the resistance to taking their } } own samples to their own labs is, well, pretty intense. They got too used } } to having us do it for them, but then what happens is they might come in } } and want to review a project from years ago. If we can't find the exact } } set of specimens, blocks, or grids on short notice, it can get dicey. } } } } We are considering instituting a storage fee to provide a little nudge to } } get people to take their own stuff back. Fees are very effective behavior } } modifiers and can be used not only for this, but for discouraging } } late-shows or no-shows on scopes, messes being left behind by sloppy } } users, failure to fill out forms and logbooks, etc. We generally find we } } only have to charge a behavior-mod fee (BMF) once or twice and that } } behavior has been modded. } } } } Regarding images, we generally archive them here in digital form, which is } } not a huge problem with large, cheap hard-drives readily available. We } } used to back up images on CD's and DVD's, but now we generally leave on } } big hard drives backing up our primary hard drives on the scope } } computers. That said, we have very rarely been asked to dig up old } } images, so it may not really be necessary. It would not be unreasonable, } } in my opinion, to make the user responsible for this aspect of their work, } } too. } } } } Every lab is different and we all operate under different sets of } } instructions, but this is how we go about things in our facility. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week& } } NavType=Both&Type=TimePlan } } Sons of Norway: http://www.sofn.com } } } } } } } } } } -----Original Message----- } } X-from: rhsia-at-umaryland.edu [mailto:rhsia-at-umaryland.edu] } } Sent: Sunday, February 08, 2009 3:02 PM } } To: Tindall, Randy D. } } Subject: [Microscopy] viaWWW: Core Facility data/sample storage } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both rhsia-at-umaryland.edu as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: rhsia-at-umaryland.edu } } Name: Ru-ching Hsia } } } } Organization: U Maryland } } } } Title-Subject: [Filtered] Core Facility data/sample storage } } } } Question: Dear all, } } I have been the director of a new EM Core } } facility for just more than a year now. Besides } } dealing with the wide variety of samples and EM } } projects brought into our facility, I found } } myself confronted with a huge amount of image } } data, specimen blocks and grids, much more than } } I am used to. After keeping all the blocks and } } grids of each project for a year, we are running } } out of grid boxes and have not figured out a best } } storage and tracking system for grids. I know } } there are many EM facility directors regularly } } contribute to the List, I would like to find out } } what are the policies of other EM Core Facilities } } concerning keeping user/clientís embedded blocks, } } grids and image data? } } Do you keep all the blocks and grids after each } } project or do you give everything back to your } } clients after the completion of the project? } } If you keep the grids and blocks, how long do you } } keep them? What is the best way to store and } } index the grids from different projects? We find } } it easier to keep all the grids from one project } } in one grid box but that means we need more than } } 50 grid boxes a year. } } How about the images? Do you keep them all? Is } } there any particular software that you use to } } catalogue all the image files? } } Before I sign off, I would like to thank everyone } } in the list who generously shares his/her } } invaluable knowledge here. I was not trained as } } an electron microscopist, but was landed this job } } by a total twist of fate. The list and the } } archive have been a great resource for me. Thank } } you all. } } Sincerely, } } Ru-ching } } } } } } Login Host: 173.64.120.125 } } --------------------------------------------------------------------------- } } } } } } ==============================Original Headers============================== } } 8, 13 -- From zaluzec-at-microscopy.com Sun Feb 8 15:01:02 2009 } } 8, 13 -- Received: from [192.168.43.136] (msdvpn8.msd.anl.gov } } [130.202.238.72]) } } 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n18L0v6e017516 } } 8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 15:01:00 } } -0600 } } 8, 13 -- Mime-Version: 1.0 } } 8, 13 -- Message-Id: {p06240800c5b4f8766447-at-[10.5.0.80]} } } 8, 13 -- Date: Mon, 9 Feb 2009 07:30:54 +1030 } } 8, 13 -- To: microscopy-at-microscopy.com } } 8, 13 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver) } } 8, 13 -- Subject: viaWWW: Core Facility data/sample storage } } 8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } } 8, 13 -- Content-Transfer-Encoding: 8bit } } 8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id n18L0v6e017516 } } ==============================End of - Headers============================== } } } } } } ==============================Original Headers============================== } } 25, 30 -- From TindallR-at-missouri.edu Mon Feb 9 08:34:08 2009 } } 25, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu } } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } } 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n19EY7ef007304 } } 25, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 08:34:07 } } -0600 } } 25, 30 -- X-IronPort-Anti-Spam-Filtered: true } } 25, 30 -- X-IronPort-Anti-Spam-Result: } } ApoEAOnNj0nRauUp/2dsb2JhbADCKiMBAQEHhAWISoJjAQGBNQaFdA } } 25, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) } } ([209.106.229.41]) } } 25, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 09 Feb } } 2009 08:34:06 -0600 } } 25, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by } } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } 25, 30 -- Mon, 9 Feb 2009 08:34:06 -0600 } } 25, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 25, 30 -- Content-class: urn:content-classes:message } } 25, 30 -- MIME-Version: 1.0 } } 25, 30 -- Content-Type: text/plain; } } 25, 30 -- charset="iso-8859-1" } } 25, 30 -- Subject: RE: [Microscopy] viaWWW: Core Facility data/sample storage } } 25, 30 -- Date: Mon, 9 Feb 2009 08:34:05 -0600 } } 25, 30 -- Message-ID: } } {91108EF9255B394CBF8B7E3789814A4103CD7DA6-at-UM-XMAIL08.um.umsystem.edu} } } 25, 30 -- In-Reply-To: {200902082102.n18L2RAF019107-at-ns.microscopy.com} } } 25, 30 -- X-MS-Has-Attach: } } 25, 30 -- X-MS-TNEF-Correlator: } } 25, 30 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample } } storage } } 25, 30 -- Thread-Index: AcmKMI0d/imrqTxSTNe2aPrAmz2cEQAkPpmw } } 25, 30 -- References: {200902082102.n18L2RAF019107-at-ns.microscopy.com} } } 25, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } 25, 30 -- To: {rhsia-at-umaryland.edu} } } 25, 30 -- Cc: {microscopy-at-microscopy.com} } } 25, 30 -- X-OriginalArrivalTime: 09 Feb 2009 14:34:06.0042 (UTC) } } FILETIME=[760A6BA0:01C98AC3] } } 25, 30 -- Content-Transfer-Encoding: 8bit } } 25, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id n19EY7ef007304 } } ==============================End of - Headers============================== } } Alan W Nicholls, PhD } Interim Associate Director - RRC } Director of Research Service Facility (Electron Microscopy) } Research Resources Center - East (M/C 337) } Room 110 Science and Engineering South Building } The University of Illinois at Chicago } 845 West Taylor St } Chicago, IL 60607-7058 } } Tel: 312 996 1227 } Fax: 312 996 8091 } Office: Room 110 } } Web site www.rrc.uic.edu } } } ==============================Original Headers============================== } 10, 20 -- From nicholls-at-uic.edu Mon Feb 9 09:10:33 2009 } 10, 20 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu } [128.248.155.184]) } 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n19FAWmG003455 } 10, 20 -- for {Microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 7, 32 -- From dsherman-at-purdue.edu Mon Feb 9 12:03:51 2009 7, 32 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19I3peM009333 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 12:03:51 -0600 7, 32 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 7, 32 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n19I3pNl009425 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51 -0500 7, 32 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 7, 32 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n19I3ol2002766 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51 -0500 7, 32 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 32 -- Mon, 9 Feb 2009 13:03:51 -0500 7, 32 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ; 7, 32 -- Mon, 9 Feb 2009 18:03:50 +0000 7, 32 -- User-Agent: Microsoft-Entourage/12.15.0.081119 7, 32 -- Date: Mon, 09 Feb 2009 13:03:49 -0500 7, 32 -- Subject: Re: [Microscopy] viaWWW: Core Facility data/sample storage 7, 32 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 32 -- To: Alan Nicholls {nicholls-at-uic.edu} , 7, 32 -- "message to: MSA list" {microscopy-at-microscopy.com} 7, 32 -- Message-ID: {C5B5DA35.3AA90%dsherman-at-exchange.purdue.edu} 7, 32 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample storage 7, 32 -- Thread-Index: AcmK4MIRSkmQsvSMRgyyuluGmbg8tw== 7, 32 -- In-Reply-To: {200902091513.n19FDQRU006632-at-ns.microscopy.com} 7, 32 -- Mime-version: 1.0 7, 32 -- Content-type: text/plain; 7, 32 -- charset="ISO-8859-1" 7, 32 -- X-OriginalArrivalTime: 09 Feb 2009 18:03:51.0185 (UTC) FILETIME=[C35ED810:01C98AE0] 7, 32 -- X-PMX-Version: 5.4.0.320885 7, 32 -- X-PerlMx-Virus-Scanned: Yes 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19I3peM009333 ==============================End of - Headers==============================
On Feb 9, 2009, at 9:43 AM, malcolm.haswell-at-sunderland.ac.uk wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It's beginning to sound as if the mechanical shutter which closes, } opens, then closes again for the exposure has failed. It's a long time } since I've used a 300 but I'm sure I had a similar problem with an } even older AEI 801 and if I remember rightly a lead had become } dislodged from the vicinity of the shutter mechanism at the back of } the microscope column. } } A lot of the older shutters have a characteristic noise as the } mechanism works, this may give a clue if it's stopped happening. } } If you have an image that is visible before photography and nothing } happens during the exposure time, then the only other thing worth } trying is perhaps experimenting with exposure times to see if it's } just one setting that has failed. } } You may well need to contact an engineer so unless you already know } one who services Philips EM300s, this might be a good time to find } someone because you may need him/her again. }
Right, this sounds a lot like a problem I had on a TEM once. Eventually I learned to look at the edge of the viewing screen to see if the shutter was opening. Dumb me didn't really get how the shutter was working during the exposure. As Malcolm describes, you should be able to see the shutter close (dark screen) before the screen goes up, a brief blink of light when the shutter opens, followed by another dark screen before the screen goes down.
If the scope is also equipped with a digital camera, there is always the chance that someone has diddled the shutter circuit and there is a mistake someplace. It happened to me when a camera was removed and the wire to the shutter was not taken away.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 10, 44 -- From jkrupp-at-deltacollege.edu Mon Feb 9 12:04:00 2009 10, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 10, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n19I40hB009462 10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 12:04:00 -0600 10, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 10, 44 -- by localhost (Email Security Appliance) with SMTP id EC9F0213F8C_9906AA5B 10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 17:40:53 +0000 (GMT) 10, 44 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 10, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id BE1EC16E67F_9906AA5F 10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 17:40:53 +0000 (GMT) 10, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 10, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 10, 44 -- with ESMTP id 45484822 for Microscopy-at-microscopy.com; Mon, 09 Feb 2009 10:03:58 -0800 10, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 10, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 10, 44 -- ESMTP id KET84200.QE7 for {Microscopy-at-microscopy.com} ; Mon, 9 10, 44 -- Feb 2009 09:48:02 -0800 10, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 7EBAA8F744D3 10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:03:58 -0800 (PST) 10, 44 -- X-Virus-Scanned: amavisd-new at 10, 44 -- X-Spam-Flag: NO 10, 44 -- X-Spam-Score: -2.48 10, 44 -- X-Spam-Level: 10, 44 -- X-Spam-Status: No, score=-2.48 tagged_above=-10 required=6 tests=[AWL=0.019, 10, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1] 10, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 44 -- with ESMTP id wCI-evphjeCg for {Microscopy-at-microscopy.com} ; 10, 44 -- Mon, 9 Feb 2009 10:03:57 -0800 (PST) 10, 44 -- Received: from [172.20.2.193] (unknown [172.20.2.193]) 10, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C16978F73E7F 10, 44 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 10:03:57 -0800 (PST) 10, 44 -- Message-Id: {5A413F68-AC4C-43F0-9A4B-0BE408EAB0BF-at-deltacollege.edu} 10, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 10, 44 -- To: Microscopy-at-microscopy.com 10, 44 -- In-Reply-To: {200902091743.n19HhJ3b006840-at-ns.microscopy.com} 10, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 10, 44 -- Content-Transfer-Encoding: 7bit 10, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3) 10, 44 -- Subject: Re: [Microscopy] Philips EM300 plate film: no image on negatives 10, 44 -- Date: Mon, 9 Feb 2009 10:03:57 -0800 10, 44 -- References: {200902091743.n19HhJ3b006840-at-ns.microscopy.com} 10, 44 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
We need to make some distinctions here. One might hope to extract from an electron diffraction pattern: (a) The positions of each diffraction peak. This gives a measure of the d-spacings (lattice constants) in the crystal (b) The intensity of each diffraction peak. This may be used through some form of refinement technique or direct methods to work out the atomic positions within the lattice.
Within case (a), we should further distinguish absolute from relative measurements. Absolute measurements occur when we try to determine the lattice constants of an unknown crystal based on its pattern. Relative measurements occur when we try to compare measurements within a single specimen.
It seems to me that the absolute error in measuring a crystal d- spacing from a transmission electron diffraction pattern in a TEM has not changed much in the last decade. The major sources of error are still the same: (i) The position of the specimen varies slightly from specimen to specimen. This change results in a change in objective lens strength for focussed electron diffraction patterns. The change in focus changes the effective camera length. (ii) Hysteresis in the ferromagnetic pole pieces of the intermediate and projector lenses means that the precise camera length is a function of each of the intermediate lens settings for the last several changes of image magnification, and image/diffraction modes. (iii) Distortions associated with off-axis aberrations in the intermediate and projector lenses are likely to cause errors when large g's are used to decrease relative error. For modern microscopes, reasonably well tuned up, this absolute error is about 1%.
Relative errors measured under the most favorable conditions are also pretty much what they were ten years ago. In my opinion, the best measurement of relative error is done using CBED HOLZ lines from a single crystal of optimum thickness. Changes of lattice constant within such a crystal (as might occur from alloying) can be detected at the 10^-5 level. The HOLZ lines are very narrow, so that slight shifts can be measured very accurately. The dark HOLZ lines in the forward disc come down the column near the optic axis so that they are not much affected by off-axis aberrations. One pitfall with CBED is that dynamical effects may cause HOLZ line shifts for reasons other than lattice parameter changes.
For less-than optimal conditions, relative error can be minimized by making the beam very parallel and going to large g. We tend to see splitting at higher orders (large g) because (i) the peaks are intrinsically narrower, (ii) the small shifts accumulate, and (iii) there is less diffuse background. Use of large g reduces relative errors, but only up to a point. At very high scattering angle the signal becomes too weak, and off-axis aberrations start to become important.
In any case, for parallel-beam diffraction, there will ultimately be a need to separate two closely spaced diffraction maxima. Detectors with good spatial discrimination are essential. However pixel size in CCD cameras has not diminished much over the last decade. Manufacturers assume that you will be able to use your intermediate and projector lenses to produce adequate magnifications or camera lengths. Software analysis of diffraction patterns recorded with digital cameras might give some modest improvement in background subtraction or peak separation provided that the fundamental signal is adequate. Using the best available intermediate lenses, digital cameras, and image analysis software might allow you to achieve relative errors as low as 1 X 10^-4, but normally one should expect something somewhat larger, say 5 X 10^-4.
b) Intensity measurements for structure determinations have to deal with (i) data collection, (ii) dynamical effects, and (iii) the phase problem in crystallography. By data collection, I refer to (i) the difficulties encountered in trying to extract the elastically scattered electrons from the diffuse background, and (ii) issues of deviation from Bragg angle. One can energy-filter the diffraction pattern, but that will not eliminate those electrons that create phonons in the specimen and lose tenths of eV's. Low T and high KV help but do not totally solve this problem. Because electron diffraction requires thin specimens, the diffraction equations are relaxed such that intensity occurs off the Bragg angle. Measuring the total intensity of a beam requires some form of integration over a volume of reciprocal space. Off the top of my head, I'm not aware of anyone trying to do that successfully. Electrons undergo multiple diffractions (phase shifts greater than 2*pi) within thicker specimens. This means that measured intensity is not simply related to the square of the structure factor. The thickness and precise orientation of the specimen have to be factored in, and they are generally not sufficiently well known. Thus, many of the techniques developed for weak scatterers, like X-rays, do not work. This is fundamental physics; technology has not touched it. Finally, even if we can measure intensity and assume that it is proportional to the structure factor times its complex conjugate, we still do not measure the phase of the structure factor. X-ray people make up for this lack of phase information using a variety of techniques that, ultimately, rely on the redundancy of diffraction data. They either create model structures and simulate the diffraction patterns or (ii) use direct methods. As Bill mentioned, Dorset and others have managed to overcome these difficulties and solve some organic structures. In effect they work under conditions that make electrons behave more like X-rays. At very high energies, with very-low-atomic-number specimens, electrons are not likely to be so dynamic, diffuse scatter will be low, and the deviations from Bragg are not very different among the beams used for analysis. Under these conditions, the approximations and calculational algorithms developed for X-rays can work.
For those of us who work with thicker, small-unit-call, higher-atomic- number, specimens at 200keV, our best hope for structure determination is to use high resolution images. Aberration correctors and/or focal series reconstructions may be helpful. But your question was directed toward electron diffraction...
This has gotten rather long. Perhaps it might compete for the longest single contribution to the microscopy list server.
(In fact, I'm not sure that I know how to upload this to the list server. It's well past 5 pm on a Friday; I'm disinclined to remind myself how to do it. If either of you think this might be of more general interest, feel free to put it up for me.)
Lew Rabenberg University of Texas
On Jan 30, 2009, at 12:06 PM, tivol-at-caltech.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Jan 30, 2009, at 9:06 AM, contact-at-integrityscientific.com wrote: } } } I would like to get some information on TEM diffraction pattern } } analysis. Specifically; } } } } 1) What software is available for analysis of diffraction patterns } } (both } } ring patterns and spot patterns)? What kind of accuracy can be } } obtained } } - are we getting close to the accuracy of X-ray diffractometry yet, } } or } } are there fundamental reasons such as lens aberrations, smaller Bragg } } angles, and accuracy of measurement which mean that we'll never get } } there? } } } } 2) What are the typical procedures people use for, say, measuring } } camera } } length or identifying unknown phases using diffraction? } } } Dear Richard, } I certainly do not know all the existing software, but I do know that } there is the SP operation in SPIDER that can identify lattice points, } find the centers and radii of rings, and determine intensity values. } When I was doing SAED to determine the structure of phthalocyanines, I } wrote a script that performed a background subtraction in two steps. } The first step put boxes around all the spots, replaced the pixels } inside the boxes with values that were the average of the edges of the } boxes, then took a radial average (the center of which was the center } of the lattice). This was then subtracted from the ED pattern, which } got rid of the non-linear background and didn't have to be exact, } since the second step was a bilinear background subtraction. The } resulting intensities were sufficiently accurate to give reliable } structure determinations. I published several articles with Doug } Dorset and Jim Turner about this in the early '90s. Neither lens } aberrations, nor small Bragg angles present practical problems for } structure determinations, but dynamical scattering is a serious issue, } which was overcome in my work by operating at 1200 kV, which reduced } dynamical effects to a manageable level. Although I have never done } any CBED, I have heard reports at M&M detailing the information that } can be obtained, and these said that the low-order spots gave the most } accurate data on such features as the distribution of electrons in } chemical bonds. these data were more accurate than could be obtained } by any other method, including X-ray diffraction. John Spence is the } expert on this, so you may want to contact him. } I evaporated gold onto the phthalocyanines and took SAED patterns } from which the lattice constants of the phthalocyanines were } determined. (This also determines the camera length.) Having both } the gold and the specimen on the same grid controls for changes in } camera length that may occur with variations in specimen height or } other scope parameters. The phases were found by direct methods--in } the case of the phthalocyanines, the Sayre equation and triplet } formulas were sufficient, but either the tangent formula or maximum } entropy methods should work also. I authored a paper in Acta Cryst. } showing that these methods will work for electron diffraction, since } they are a consequence of the unitary nature of scattering processes. } The references in that paper are to work done by several people to } which I added a small amount. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu }
==============================Original Headers============================== 17, 22 -- From tivol-at-caltech.edu Mon Feb 9 13:56:52 2009 17, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19Juqlc013114 17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:56:52 -0600 17, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 17, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id D47CE32A288 17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 11:56:51 -0800 (PST) 17, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 17, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 17, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 46B5E329F96 17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 11:56:50 -0800 (PST) 17, 22 -- Message-Id: {E2EDD153-8281-4558-A305-39155A41037B-at-caltech.edu} 17, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 17, 22 -- To: microscopy-at-microscopy.com 17, 22 -- In-Reply-To: {200901301806.n0UI6rlk028308-at-ns.microscopy.com} 17, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 17, 22 -- Content-Transfer-Encoding: 7bit 17, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 17, 22 -- Subject: Re: [Microscopy] Re: TEM; diffraction pattern analysis 17, 22 -- Date: Mon, 9 Feb 2009 11:56:49 -0800 17, 22 -- References: {200901301806.n0UI6rlk028308-at-ns.microscopy.com} 17, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Eons ago, when I worked in a clinical TEM lab, we stored everything forever. To save space and the cost of expensive grid boxes, we would archive the grids by putting them in labeled small beem capsules, that were stored in the same white slide box as the tissue blocks. That allowed us to reuse the grid boxes, which we labeled by writing on scotch tape attached to the sliding lid.
The research TEM lab here has investigators take their blocks/grids/slides/negatives back and people are responsible for the long-term archiving of their digital images.
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Monday, February 09, 2009 11:05 AM To: dcromey-at-email.arizona.edu
I agree with giving sample blocks, grids, data, etc to the investigators immediately after completing the project. We cannot/should not use them and we should not be responsible for their safe keeping. That is the responsibility of the investigators.
We do backup service project digital images and hold them for a few months. Then I will write DVDs and store those. We also backup independent user digital images for a while but periodically ask them to clear all images off our computers. They are told that they should copy images onto portable media immediately after each session. We hold no responsibility for loss if a hard drive fails, etc and will not go to extraordinary effort to reclaim lost data.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: Alan Nicholls {nicholls-at-uic.edu} } Reply-To: Alan Nicholls {nicholls-at-uic.edu} } Date: Mon, 9 Feb 2009 09:13:26 -0600 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] viaWWW: Core Facility data/sample storage } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ru-ching } } I agree totally with Randy. Once the project has been completed we expect } the users to take and store their own specimens. We also expect all users } to archive and store their own data. We started, 11 years ago, by also } storing them in digital form before taking them off instrument computers, } but after a few years the number of CD-R disks was becoming impossible. } There were only a very few occasions that users asked for the archived } images so we no longer do it. } } Regards } } Alan } } At 08:35 AM 2/9/2009, TindallR-at-missouri.edu wrote: } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Ru-ching, } } } } I would strongly recommend having your users take and store their own } } samples themselves, and the earlier you start having them do it, the better. } } } } For years, we had been keeping samples in storage for our clients, until } } the volume became overwhelming. Samples, grids,blocks, etc. were piled } } everywhere. We are now in the very slow process of getting the samples } } back to those researchers who still want them. The problem is that in } } some cases, especially high-volume users, the resistance to taking their } } own samples to their own labs is, well, pretty intense. They got too used } } to having us do it for them, but then what happens is they might come in } } and want to review a project from years ago. If we can't find the exact } } set of specimens, blocks, or grids on short notice, it can get dicey. } } } } We are considering instituting a storage fee to provide a little nudge to } } get people to take their own stuff back. Fees are very effective behavior } } modifiers and can be used not only for this, but for discouraging } } late-shows or no-shows on scopes, messes being left behind by sloppy } } users, failure to fill out forms and logbooks, etc. We generally find we } } only have to charge a behavior-mod fee (BMF) once or twice and that } } behavior has been modded. } } } } Regarding images, we generally archive them here in digital form, which is } } not a huge problem with large, cheap hard-drives readily available. We } } used to back up images on CD's and DVD's, but now we generally leave on } } big hard drives backing up our primary hard drives on the scope } } computers. That said, we have very rarely been asked to dig up old } } images, so it may not really be necessary. It would not be unreasonable, } } in my opinion, to make the user responsible for this aspect of their work, } } too. } } } } Every lab is different and we all operate under different sets of } } instructions, but this is how we go about things in our facility. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week & } } NavType=Both&Type=TimePlan } } Sons of Norway: http://www.sofn.com } } } } } } } } } } -----Original Message----- } } X-from: rhsia-at-umaryland.edu [mailto:rhsia-at-umaryland.edu] } } Sent: Sunday, February 08, 2009 3:02 PM } } To: Tindall, Randy D. } } Subject: [Microscopy] viaWWW: Core Facility data/sample storage } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both rhsia-at-umaryland.edu as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: rhsia-at-umaryland.edu } } Name: Ru-ching Hsia } } } } Organization: U Maryland } } } } Title-Subject: [Filtered] Core Facility data/sample storage } } } } Question: Dear all, } } I have been the director of a new EM Core } } facility for just more than a year now. Besides } } dealing with the wide variety of samples and EM } } projects brought into our facility, I found } } myself confronted with a huge amount of image } } data, specimen blocks and grids, much more than } } I am used to. After keeping all the blocks and } } grids of each project for a year, we are running } } out of grid boxes and have not figured out a best } } storage and tracking system for grids. I know } } there are many EM facility directors regularly } } contribute to the List, I would like to find out } } what are the policies of other EM Core Facilities } } concerning keeping user/clientís embedded blocks, } } grids and image data? } } Do you keep all the blocks and grids after each } } project or do you give everything back to your } } clients after the completion of the project? } } If you keep the grids and blocks, how long do you } } keep them? What is the best way to store and } } index the grids from different projects? We find } } it easier to keep all the grids from one project } } in one grid box but that means we need more than } } 50 grid boxes a year. } } How about the images? Do you keep them all? Is } } there any particular software that you use to } } catalogue all the image files? } } Before I sign off, I would like to thank everyone } } in the list who generously shares his/her } } invaluable knowledge here. I was not trained as } } an electron microscopist, but was landed this job } } by a total twist of fate. The list and the } } archive have been a great resource for me. Thank } } you all. } } Sincerely, } } Ru-ching } } } } } } Login Host: 173.64.120.125 } } --------------------------------------------------------------------------- } } } } } } ==============================Original Headers============================== } } 8, 13 -- From zaluzec-at-microscopy.com Sun Feb 8 15:01:02 2009 } } 8, 13 -- Received: from [192.168.43.136] (msdvpn8.msd.anl.gov } } [130.202.238.72]) } } 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n18L0v6e017516 } } 8, 13 -- for {microscopy-at-microscopy.com} ; Sun, 8 Feb 2009 15:01:00 } } -0600 } } 8, 13 -- Mime-Version: 1.0 } } 8, 13 -- Message-Id: {p06240800c5b4f8766447-at-[10.5.0.80]} } } 8, 13 -- Date: Mon, 9 Feb 2009 07:30:54 +1030 } } 8, 13 -- To: microscopy-at-microscopy.com } } 8, 13 -- From: rhsia-at-umaryland.edu (by way of MicroscopyListserver) } } 8, 13 -- Subject: viaWWW: Core Facility data/sample storage } } 8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } } 8, 13 -- Content-Transfer-Encoding: 8bit } } 8, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id n18L0v6e017516 } } ==============================End of - Headers============================== } } } } } } ==============================Original Headers============================== } } 25, 30 -- From TindallR-at-missouri.edu Mon Feb 9 08:34:08 2009 } } 25, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu } } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } } 25, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n19EY7ef007304 } } 25, 30 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 7, 32 -- From dsherman-at-purdue.edu Mon Feb 9 12:03:51 2009 7, 32 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19I3peM009333 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 12:03:51 -0600 7, 32 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 7, 32 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n19I3pNl009425 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51 -0500 7, 32 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 7, 32 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n19I3ol2002766 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 13:03:51 -0500 7, 32 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 32 -- Mon, 9 Feb 2009 13:03:51 -0500 7, 32 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ; 7, 32 -- Mon, 9 Feb 2009 18:03:50 +0000 7, 32 -- User-Agent: Microsoft-Entourage/12.15.0.081119 7, 32 -- Date: Mon, 09 Feb 2009 13:03:49 -0500 7, 32 -- Subject: Re: [Microscopy] viaWWW: Core Facility data/sample storage 7, 32 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 32 -- To: Alan Nicholls {nicholls-at-uic.edu} , 7, 32 -- "message to: MSA list" {microscopy-at-microscopy.com} 7, 32 -- Message-ID: {C5B5DA35.3AA90%dsherman-at-exchange.purdue.edu} 7, 32 -- Thread-Topic: [Microscopy] viaWWW: Core Facility data/sample storage 7, 32 -- Thread-Index: AcmK4MIRSkmQsvSMRgyyuluGmbg8tw== 7, 32 -- In-Reply-To: {200902091513.n19FDQRU006632-at-ns.microscopy.com} 7, 32 -- Mime-version: 1.0 7, 32 -- Content-type: text/plain; 7, 32 -- charset="ISO-8859-1" 7, 32 -- X-OriginalArrivalTime: 09 Feb 2009 18:03:51.0185 (UTC) FILETIME=[C35ED810:01C98AE0] 7, 32 -- X-PMX-Version: 5.4.0.320885 7, 32 -- X-PerlMx-Virus-Scanned: Yes 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19I3peM009333 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 28 -- From dcromey-at-email.arizona.edu Mon Feb 9 14:18:23 2009 22, 28 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n19KIMMA027429 22, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 14:18:22 -0600 22, 28 -- Received: from gandalfs_amavis (amavis8.email.arizona.edu [10.0.0.236]) 22, 28 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 0499B9BA7BF 22, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 13:18:22 -0700 (MST) 22, 28 -- Received: from RASTER (hepatic1.cba.arizona.edu [128.196.157.6]) 22, 28 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 8FCEF9BA7C9 22, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 9 Feb 2009 13:18:19 -0700 (MST) 22, 28 -- Reply-To: {cromey-at-arizona.edu} 22, 28 -- From: "Doug Cromey" {dcromey-at-email.arizona.edu} 22, 28 -- To: "Microscopy listserv" {Microscopy-at-Microscopy.Com} 22, 28 -- References: {200902091804.n19I4edN011036-at-ns.microscopy.com} 22, 28 -- In-Reply-To: {200902091804.n19I4edN011036-at-ns.microscopy.com} 22, 28 -- Subject: RE: [Microscopy] Re: viaWWW: Core Facility data/sample storage 22, 28 -- Date: Mon, 9 Feb 2009 13:18:18 -0700 22, 28 -- Organization: University of Arizona 22, 28 -- Message-ID: {003601c98af3$8d324de0$a796e9a0$-at-arizona.edu} 22, 28 -- MIME-Version: 1.0 22, 28 -- Content-Type: text/plain; 22, 28 -- charset="iso-8859-1" 22, 28 -- X-Mailer: Microsoft Office Outlook 12.0 22, 28 -- Thread-Index: AcmK4OQKYRUd5ruSS1yj29DwEiPrLQAEqLTg 22, 28 -- Content-Language: en-us 22, 28 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 22, 28 -- Content-Transfer-Encoding: 8bit 22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n19KIMMA027429 ==============================End of - Headers==============================
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] Staining Proteins for TEM
Question: Over a month ago I sent out a message indicating that I would like to know how to stain proteins for enhanced contrast in TEM imaging. I have no prior experience with imaging biological samples, so I would appreciate any helpful comments. I received a number of helpful suggestions, including the observation that I would benefit by giving more of the details of my proposed experiment so that the people could give me advice more specific to my needs.
So, in an attempt to compose a better description of my problem, let me begin by saying that I am studying the Cytochrome c protein. 12 mg of the protein is dissolved in 1 mL of 100 mM Tris buffer. For part of this project I would just like to look at a drop of this solution on a holy carbon grid. So I would like to know how to stain the proteins that I have deposited on this carbon grid. One of my collaborators has suggested osmium tetroxide as the staining agent. I am using a 300 kV TEM to image the proteins.
In the second part of this project I will be trying to image the proteins within a carbon nanotube array. I have already succeeding in imaging this structure, as reported in our recent paper (Lebedev et al. Langmuir 24 (2008) 8871), using the gold nanoparticles attached to the protein to at least mark the presence of the proteins within the nanotubes.
For the moment, I would be happy to just image the proteins on a carbon grid. Latter on I will concern myself with imaging the proteins within the carbon nanotubes.
I'm sorry but I don't have any decent images. I can recommend the following for good pics...
1. A review in the journal Dermatology Itin, et al. Hair Shaft Abnormalities - Clues to Diagnosis and Treatment. Dermatology 2005;211:63-71
2. The skin chapter in the following book Papadimitriou, et al. Diagnostic Ultrastructure of Non-Neoplastic Diseases.
Hi John, Would it be possible to have these images. Best regards, Mansour
-----Original Message----- X-from: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au] Sent: Monday, February 09, 2009 8:17 AM To: Shafei, Mansour A
Hi Andrea,
Our SEM studies of hair are purely morphological. We look for things like twists, kinks, knots, longitudinal impressions, or even invaginations of the hair shaft (so-called "bamboo hair"). We also look at the cuticle for signs of loss or fracture. Hair shaft abnormalities may be associated with skin disorders or inborn errors of metabolism. Bear in mind that exogenous factors can also affect hair morphology.
We also look at hair using polarised light which may produce lovely banding patterns, eg, the disease called trichothiodystrophy produces alternating light and dark bands under polarised light (so-called "tiger-tail" pattern). It's thought to be the result of low sulphur content within the hair.
Regards,
John
Hi John,
I am curious about the baby hair you look at. Are you looking for morphological abnormalities, elemental composition, or both? I have never heard of this before.
Thanks, Andrea
Andrea Blake Brothers Senior Scientist, MMCC Microscopy & Microanalysis Characterization Center andrea.brothers-at-biovail.com
(703) 480-5879 office (703) 480-5943 fax
BIOVAIL 3701 Concorde Parkway Chantilly, VA 20151
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-----Original Message----- X-from: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au] Sent: Thursday, February 05, 2009 6:01 PM To: Andrea Brothers
Hi Jon,
We have an old Hitachi S-520 SEM still operational. Once or twice a year we are asked to look at paediatric hair specimens for defects. Of course, babies can't tell what's wrong with them so looking at hair samples can sometimes give you a clue as to what is the problem. Ie, the hair defect may be one part of a wider syndrome.
Regards,
John Brealey
Senior Medical Scientist, Electron Microscopy Unit
T 08 8222 6612 F 08 8222 6425
www.sapathology.sa.gov.au
SA Pathology (TQEH) Quality Pathology supporting Training and Research
==============================Original Headers============================== 47, 27 -- From john.brealey-at-imvs.sa.gov.au Mon Feb 9 18:04:05 2009 47, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13]) 47, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1A041Hw029815 47, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 18:04:04 -0600 47, 27 -- X-IronPort-AV: E=Sophos;i="4.37,407,1231075800"; 47, 27 -- d="scan'208";a="9535711" 47, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88]) 47, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 10 Feb 2009 10:33:58 +1030 47, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 47, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id 47, 27 -- 8.1.263.0; Tue, 10 Feb 2009 10:34:45 +1030 47, 27 -- Received: from 41347i (unknown [10.21.84.89]) by ablett.imvs.sa.gov.au 47, 27 -- (Postfix) with ESMTP id 6A53134DCE for {Microscopy-at-microscopy.com} ; Tue, 10 47, 27 -- Feb 2009 10:33:58 +1030 (CST) 47, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 47, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 47, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 47, 27 -- Subject: SEM of Hair 47, 27 -- Date: Tue, 10 Feb 2009 10:33:58 +1030 47, 27 -- Organization: IMVS 47, 27 -- Message-ID: {000601c98b13$122cdfa0$5954150a-at-41347i} 47, 27 -- MIME-Version: 1.0 47, 27 -- Content-Type: text/plain; charset="us-ascii" 47, 27 -- Content-Transfer-Encoding: 7bit 47, 27 -- X-Mailer: Microsoft Office Outlook 11 47, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 47, 27 -- thread-index: AcmLExIETaDV/NAJTvGug3UrtZtXQg== ==============================End of - Headers==============================
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Email: minwen-at-u.washington.edu Name: Min Spencer
Organization: UW Seattle
Title-Subject: [Filtered] Cacodylate Buffer
Question: We do the perfusion for EM of brain. We use Cacodylate Buffer. I knew that the Cacodylate Buffer is not good to the environment. Can we change to the PBS or other buffers?
Good idea, only it is not PBS but PB. The difference is no NaCl in PB and more phosphate - usually 0.05-0.1M, but use the same concentration and pH as in your cacodylate protocol. PB most commonly used for EM fixation is of both salts being Na salts, often referred to as Sorensen's. You can look the specifics up in many sources.
"Performance" difference is said to be that cacodylate tends to give a more clear view (= more extraction), and PB *sometimes* causes a precipitate, either interacting with UA or for unclear reason.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
} Email: minwen-at-u.washington.edu } Name: Min Spencer } } Organization: UW Seattle } } Title-Subject: [Filtered] Cacodylate Buffer } } Question: We do the perfusion for EM of brain. We use Cacodylate } Buffer. I knew that the Cacodylate Buffer is not good to the } environment. Can we change to the PBS or other buffers? } } Thanks } } Min Spencer } } Login Host: 128.208.64.65 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Mon Feb 9 23:07:46 2009 } 8, 11 -- Received: from [203.35.254.75] (msdvpn072.msd.anl.gov } [130.202.238.72]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n1A57XJ4019355 } 8, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 } 23:07:40 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c5b6bc04d7d3-at-[192.168.43.136]} } 8, 11 -- Date: Tue, 10 Feb 2009 15:37:29 +1030 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: minwen-at-u.washington.edu (by way of } MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Cacodylate Buffer } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From vladislav_speransky-at-nih.gov Mon Feb 9 23:30:39 2009 8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1A5Udxa001231 8, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Feb 2009 23:30:39 -0600 8, 23 -- Received: from compute1.internal (compute1.internal [10.202.2.41]) 8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id CD97B29055B 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 00:30:38 -0500 (EST) 8, 23 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160]) 8, 23 -- by compute1.internal (MEProxy); Tue, 10 Feb 2009 00:30:38 -0500 8, 23 -- X-Sasl-enc: G6PHINPA0+O9+eZxcGrNxaFAcTDZVNXkj0Holf6Uzce6 1234243838 8, 23 -- Received: from [192.168.0.5] (pool-173-66-189-48.washdc.fios.verizon.net [173.66.189.48]) 8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id 7CE3831375 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 00:30:38 -0500 (EST) 8, 23 -- Message-Id: {D8C79492-7112-4F51-BCC3-3F53BDDDC8F6-at-nih.gov} 8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 8, 23 -- Subject: Fwd: [Microscopy] viaWWW: Cacodylate Buffer 8, 23 -- Date: Tue, 10 Feb 2009 00:30:37 -0500 8, 23 -- References: {200902100508.n1A58rot020685-at-ns.microscopy.com} 8, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
We are looking to purchase some good microscopy reference books or atlases. I know this topic has come up before, but I would like to know what people recommend.
Thanks! Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
==============================Original Headers============================== 9, 26 -- From MSHERWOOD-at-PARTNERS.ORG Tue Feb 10 13:38:54 2009 9, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AJcsPA030977 9, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 13:38:54 -0600 9, 26 -- X-IronPort-AV: E=Sophos;i="4.38,187,1233550800"; 9, 26 -- d="scan'208";a="59281141" 9, 26 -- Received: from phsxcon1.mgh.harvard.edu (HELO PHSXCON1.partners.org) ([132.183.130.40]) 9, 26 -- by phsmgmx11.partners.org with ESMTP; 10 Feb 2009 14:38:54 -0500 9, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 9, 26 -- Tue, 10 Feb 2009 14:38:53 -0500 9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 26 -- Content-class: urn:content-classes:message 9, 26 -- MIME-Version: 1.0 9, 26 -- Subject: [Microscopy] Re: Microscopy Reference Books/Atlases 9, 26 -- Date: Tue, 10 Feb 2009 14:38:53 -0500 9, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9703E237F1-at-PHSXMB30.partners.org} 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- Thread-Topic: [Microscopy] Re: Microscopy Reference Books/Atlases 9, 26 -- Thread-Index: AcmLtzSGVT+XsttPShyD1IJxxj+s1w== 9, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 9, 26 -- To: {Microscopy-at-microscopy.com} 9, 26 -- X-OriginalArrivalTime: 10 Feb 2009 19:38:53.0927 (UTC) FILETIME=[34E1F770:01C98BB7] 9, 26 -- Content-Type: text/plain; charset="us-ascii" 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1AJcsPA030977 ==============================End of - Headers==============================
I need to use JB-4 embedding resin for a project. I will be embedding and cross-sectioning cells grown in a polymer. I have never used JB-4 and would like to hear from members who have used it: protocols, staining, etc.
Thanks! Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
==============================Original Headers============================== 8, 26 -- From MSHERWOOD-at-PARTNERS.ORG Tue Feb 10 13:42:41 2009 8, 26 -- Received: from phsmgmx10.partners.org (phsmgmx10.partners.org [155.52.251.101]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AJgfAq003368 8, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 13:42:41 -0600 8, 26 -- X-IronPort-AV: E=Sophos;i="4.38,187,1233550800"; 8, 26 -- d="scan'208";a="209582819" 8, 26 -- Received: from phsxcon1.mgh.harvard.edu (HELO PHSXCON1.partners.org) ([132.183.130.40]) 8, 26 -- by phsmgmx10.partners.org with ESMTP; 10 Feb 2009 14:42:41 -0500 8, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Tue, 10 Feb 2009 14:42:40 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Subject: [Microscopy] Re: JB-4 Embedding Resin 8, 26 -- Date: Tue, 10 Feb 2009 14:42:40 -0500 8, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9703E237F2-at-PHSXMB30.partners.org} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: [Microscopy] Re: JB-4 Embedding Resin 8, 26 -- Thread-Index: AcmLt7v89s6G+kLCREiQOlGR5l1tyA== 8, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 10 Feb 2009 19:42:41.0111 (UTC) FILETIME=[BC4B7E70:01C98BB7] 8, 26 -- Content-Type: text/plain; charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1AJgfAq003368 ==============================End of - Headers==============================
We have a limited number of carbon rods left. As you indicated they are dark and hard.
For our coated grids we use the highly purified graphite (soft, silvery gray). It coats in our evaporator at between 18 and 20 amps. It produces a very smooth film for our carbon and formvar coated grids.
We have some left over carbon rods. Let me know.
John Arnott
Disclaimer: Ladd Research sells EM supplies including carbon rods
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==============================Original Headers============================== 15, 27 -- From jd-at-laddresearch.com Tue Feb 10 14:53:16 2009 15, 27 -- Received: from anders.electric.net (anders.electric.net [72.35.23.15]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AKrFPe029651 15, 27 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 14:53:16 -0600 15, 27 -- Received: from 1LWzbU-00069I-T6 by anders.electric.net with emc1-ok (Exim 4.69) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1LWzbU-0006Aq-V3; Tue, 10 Feb 2009 12:53:12 -0800 15, 27 -- Received: by emcmailer; Tue, 10 Feb 2009 12:53:12 -0800 15, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 15, 27 -- by anders.electric.net with esmtps (TLSv1:AES256-SHA:256) 15, 27 -- (Exim 4.69) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1LWzbU-00069I-T6; Tue, 10 Feb 2009 12:53:12 -0800 15, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 27 -- Date: Tue, 10 Feb 2009 15:53:05 -0500 15, 27 -- To: ahlst007-at-umn.edu 15, 27 -- From: jd {jd-at-laddresearch.com} 15, 27 -- Subject: Re: [Microscopy] Carbon Rods 15, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 15, 27 -- In-Reply-To: {200902062241.n16MfBwv004470-at-ns.microscopy.com} 15, 27 -- References: {200902062241.n16MfBwv004470-at-ns.microscopy.com} 15, 27 -- Mime-Version: 1.0 15, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 27 -- X-Outbound-IP: 216.204.198.170 15, 27 -- X-Env-From: jd-at-laddresearch.com 15, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 15, 27 -- Message-Id: {E1LWzbU-0006Aq-V3-at-anders.electric.net} ==============================End of - Headers==============================
What's the difference between carbon and graphite rods? Do certain ones make better thin films than others?
Both carbon and graphite are produced in a furnace from carbon powder.
Carbon is formed at about 900 degrees C which results in a harder, amorphous structure. It has a darker appearance and is harder. Graphite is formed at about 2500 degrees C which results in a crystalline structure. It is softer (slippery feeling) and softer. It writes a bit like a pencil.
We use a highly purified graphite for our thin film. It produces a smoother film and works well in our evaporator at a current of 18 to 20 amps.
We also have carbon rods and technical grade graphite available. The technical grade is less pure but works in metal coating and other less critical applications.
John Arnott
Disclaimer: Ladd Research sells evaporators, coated grids and carbon rods
James Swift and Son (or their descendents) seem to no longer manufacture their esteemed Point Counter.
Meiji offer one as an accessory to their range of polarising microsopes, but I haven't found out yet whether the control/counting unit is electromechanical like the old Swift model, or electronic.
It seems to me that it should be easy enough to marry an the old Swift one to a PC, with suitable software and electrical interface to the stepper.
Has anyone done this, or does anyone know of a vendor of the appropriate software and/or interface?
cheers
Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 9, 26 -- From r.sims-at-auckland.ac.nz Tue Feb 10 17:13:19 2009 9, 26 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1ANDFox031924 9, 26 -- for {microscopy-at-Microscopy.Com} ; Tue, 10 Feb 2009 17:13:19 -0600 9, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id CE770198C1 9, 26 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:13:13 +1300 (NZDT) 9, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 9, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 9, 26 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 9, 26 -- with ESMTP id pASNCUXhQZt5 for {microscopy-at-Microscopy.Com} ; 9, 26 -- Wed, 11 Feb 2009 12:13:13 +1300 (NZDT) 9, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id AA1C41987B 9, 26 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:13:13 +1300 (NZDT) 9, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 9, 26 -- To: microscopy-at-Microscopy.Com 9, 26 -- Date: Wed, 11 Feb 2009 12:13:14 +1300 9, 26 -- MIME-Version: 1.0 9, 26 -- Subject: Geological Point Counter for Modal Analysis 9, 26 -- Message-ID: {4992C0DA.7961.CDCF70-at-r.sims.auckland.ac.nz} 9, 26 -- Priority: normal 9, 26 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 26 -- Content-type: text/plain; charset=US-ASCII 9, 26 -- Content-transfer-encoding: 7BIT 9, 26 -- Content-description: Mail message body ==============================End of - Headers==============================
I forgot that in 2005 I discovered that both appropriate software and a digitally-controlled stage are, in fact, available from Conwy Valley Systems Ltd, in the UK, so no-one needs to bring them to my attention.
I will, however, still appreciate hearing from anyone who has successfully interfaced one of the old Swift stages to a PC
cheers rtch
On 10 Feb 2009 at 17:14, r.sims-at-auckland.ac.nz wrote:
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Here's one for the geologists:
James Swift and Son (or their descendents) seem to no longer manufacture their esteemed Point Counter.
Meiji offer one as an accessory to their range of polarising microsopes, but I haven't found out yet whether the control/counting unit is electromechanical like the old Swift model, or electronic.
It seems to me that it should be easy enough to marry an the old Swift one to a PC, with suitable software and electrical interface to the stepper.
Has anyone done this, or does anyone know of a vendor of the appropriate software and/or interface?
cheers
Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 22, 28 -- From r.sims-at-auckland.ac.nz Tue Feb 10 17:23:15 2009 22, 28 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1ANNF5h013401 22, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 10 Feb 2009 17:23:15 -0600 22, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 22, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 60D3A48148C 22, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:23:14 +1300 (NZDT) 22, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 22, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 22, 28 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 22, 28 -- with ESMTP id HqDg9Av8c9OQ for {microscopy-at-Microscopy.Com} ; 22, 28 -- Wed, 11 Feb 2009 12:23:14 +1300 (NZDT) 22, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 22, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 4662748142D 22, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 11 Feb 2009 12:23:14 +1300 (NZDT) 22, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 22, 28 -- To: microscopy-at-Microscopy.Com 22, 28 -- Date: Wed, 11 Feb 2009 12:23:15 +1300 22, 28 -- MIME-Version: 1.0 22, 28 -- Subject: Woops! Geological Point Counter for Modal Analysis 22, 28 -- Message-ID: {4992C333.27967.D6FA3D-at-r.sims.auckland.ac.nz} 22, 28 -- Priority: normal 22, 28 -- In-reply-to: {200902102314.n1ANEMCP000813-at-ns.microscopy.com} 22, 28 -- References: {200902102314.n1ANEMCP000813-at-ns.microscopy.com} 22, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 22, 28 -- Content-type: text/plain; charset=US-ASCII 22, 28 -- Content-transfer-encoding: 7BIT 22, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
I was just tossing around a few thoughts, and I was was wondering if anyone has experience cooling two instruments with one chiller. My thinking is that you should be able to daisy-chain the diffusion pumps together and achieve proper chilling, but the trade-off would be a decrease in the required temperature coming out of the chiller. Does the flow rate have to be augmented somehow as well?
Perhaps the better solution is to put a Y connector in the chiller lines and run the two in parallel?
--Justin.
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 31 -- From kraftpiano-at-gmail.com Tue Feb 10 18:31:30 2009 4, 31 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.249]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0VTJp029396 4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:31:30 -0600 4, 31 -- Received: by rv-out-0708.google.com with SMTP id k29so86197rvb.30 4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 16:31:29 -0800 (PST) 4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 31 -- d=gmail.com; s=gamma; 4, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject 4, 31 -- :from:to:content-type:content-transfer-encoding; 4, 31 -- bh=wKBgQ589KW6IQrXye9biIk/WzhipxgGu8sfjdx0bmWY=; 4, 31 -- b=hsp0HV96GZB3nvJ7YkW+rdivO4kcOyywL4lxN44p4VeLazBNFdAx87cTMDVHMfehRj 4, 31 -- vsFAWun8ORtL6LAXG5uDET57r6zJiM3nRPT7Msk5CNT2EIQQh6u6nHhUa69Z+lL59xzG 4, 31 -- QOGf1J6ea0UWea673IAF1YRxnqFZTAddrmoTY= 4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 31 -- d=gmail.com; s=gamma; 4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type 4, 31 -- :content-transfer-encoding; 4, 31 -- b=igGn5V5s+hHIwnY353+jrgHHVWM7ppUa1nocNAkJQ4UwoQEZLQsF7CH4dJt6+qIfhe 4, 31 -- CTaRtL5U4bA/eubxrKlm80HfXukEm0I0iFzGRoUuzE1x4iRSFqt2+JClg9vOBG/ZoTBr 4, 31 -- aT+95zDnyoTP2Rl5+Zr6mTzz023V070yxKrCc= 4, 31 -- MIME-Version: 1.0 4, 31 -- Received: by 10.141.100.15 with SMTP id c15mr3519rvm.222.1234312289521; Tue, 4, 31 -- 10 Feb 2009 16:31:29 -0800 (PST) 4, 31 -- Date: Tue, 10 Feb 2009 19:31:29 -0500 4, 31 -- Message-ID: {25e2b0d20902101631y3200acd6lf0389f51ef9a2499-at-mail.gmail.com} 4, 31 -- Subject: SEM Cooling question. 4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com} 4, 31 -- To: microscopy-at-microscopy.com 4, 31 -- Content-Type: text/plain; charset=ISO-8859-1 4, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I run a few instruments off the one cooler, they are in parallel, with individual flow-control valves and water-flowmeters.
Remember not to have your cooled water below the dew-point!
cheers rtch
On 10 Feb 2009 at 18:32, kraftpiano-at-gmail.com wrote:
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I was just tossing around a few thoughts, and I was was wondering if anyone has experience cooling two instruments with one chiller. My thinking is that you should be able to daisy-chain the diffusion pumps together and achieve proper chilling, but the trade-off would be a decrease in the required temperature coming out of the chiller. Does the flow rate have to be augmented somehow as well?
Perhaps the better solution is to put a Y connector in the chiller lines and run the two in parallel?
--Justin.
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 12, 28 -- From r.sims-at-auckland.ac.nz Tue Feb 10 18:46:42 2009 12, 28 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0kfaV010910 12, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 10 Feb 2009 18:46:41 -0600 12, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 9680D481FDB; 12, 28 -- Wed, 11 Feb 2009 13:46:40 +1300 (NZDT) 12, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 12, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 12, 28 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 12, 28 -- with ESMTP id ERS0R92fPwWI; Wed, 11 Feb 2009 13:46:40 +1300 (NZDT) 12, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 12, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 7963F481FD9; 12, 28 -- Wed, 11 Feb 2009 13:46:40 +1300 (NZDT) 12, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 12, 28 -- To: kraftpiano-at-gmail.com 12, 28 -- Date: Wed, 11 Feb 2009 13:46:41 +1300 12, 28 -- MIME-Version: 1.0 12, 28 -- Subject: Re: [Microscopy] SEM Cooling question. 12, 28 -- CC: microscopy-at-Microscopy.Com 12, 28 -- Message-ID: {4992D6C1.2468.1235DA9-at-r.sims.auckland.ac.nz} 12, 28 -- Priority: normal 12, 28 -- In-reply-to: {200902110032.n1B0WR4c030716-at-ns.microscopy.com} 12, 28 -- References: {200902110032.n1B0WR4c030716-at-ns.microscopy.com} 12, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 28 -- Content-type: text/plain; charset=US-ASCII 12, 28 -- Content-transfer-encoding: 7BIT 12, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
Justin, Daisy-chaining is not a good idea. Most systems are also cooling some of the electronics and that needs to be done with the cooler water. Also, having very warm water at the top of the second DP (and its water baffle) would be very counter-productive by allowing a great deal more back-streaming.
A "Y" is fine as long as you put 2 flowmeters on the outlets so that you can be sure each instrument is getting the proper flow. If one has a higher resistance to flow (due to mineral or corrosion build up, or just a different design), it could end up with insufficient flow.
The biggest question is whether or not the chiller has the cooling capacity for 2 instruments. A pump can be fairly easy to upgrade, but the BTU capacity of the chiller is fixed (and sometimes less than advertised). If you're anywhere near the max for the chiller, in terms of BTUs, either get a second chiller or get a larger chiller. It's not that unusual to run 2 systems off one chiller, but they must run in parallel with separate controls and the chiller must have enough BTU capacity.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Tuesday, February 10, 2009 7:34 PM To: kenconverse-at-qualityimages.biz
I was just tossing around a few thoughts, and I was was wondering if anyone has experience cooling two instruments with one chiller. My thinking is that you should be able to daisy-chain the diffusion pumps together and achieve proper chilling, but the trade-off would be a decrease in the required temperature coming out of the chiller. Does the flow rate have to be augmented somehow as well?
Perhaps the better solution is to put a Y connector in the chiller lines and run the two in parallel?
--Justin.
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
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==============================Original Headers============================== 18, 25 -- From kenconverse-at-qualityimages.biz Tue Feb 10 18:57:50 2009 18, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0vogI024700 18, 25 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:57:50 -0600 18, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com 18, 25 -- with ESMTP 18, 25 -- id {20090211005749.FZEM18810.cdptpa-omta02.mail.rr.com-at-Ken} ; 18, 25 -- Wed, 11 Feb 2009 00:57:49 +0000 18, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 25 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 18, 25 -- Subject: RE: [Microscopy] SEM Cooling question. 18, 25 -- Date: Tue, 10 Feb 2009 19:57:44 -0500 18, 25 -- Message-ID: {4212AD7FF6364BA585A485CE87AE457A-at-Ken} 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="us-ascii" 18, 25 -- X-Priority: 3 (Normal) 18, 25 -- X-MSMail-Priority: Normal 18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 18, 25 -- In-Reply-To: {200902110034.n1B0Y520000916-at-ns.microscopy.com} 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 18, 25 -- Thread-Index: AcmL4HGB81kGdvShRtSDGHtlesxOaAAAXAaw 18, 25 -- Importance: Normal 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1B0vogI024700 ==============================End of - Headers==============================
Running the 2 in parallel is better since the cooling water will be at the same temperature for both systems. The flow rate through a DP should be set so that the water coming out should be slightly warm to the touch. I think ours run ~1 gal per minute. Most chillers have pumps that can supply a fairly large water flow. They then have a pressure valve which shunts the excess flow back into the tank (kind of like the fuel pump in your car). An inline flow meter is a really useful device. It is an immediate indication if you have any kind of blockage restricting flow (e.g. algae, corrosion, etc). I also put a 1 - 5um cartridge water filter in the waterline just before it enters the instrument.
Flow through the lenses and electronics is a bit more critical. We usually set the flow so that the outside temperature of the column is right at room temperature. You have to balance the flow rate and the temperature for things to work right. Too high a flow rate (warmer water) and you get vibration which can affect the image. Too low a flow rate (cooler water) and the water flow meter will shut the lenses down. For our TEMs, we fint that a water temperature around 60- 65 deg F is about right.
Cheers, Henk
kraftpiano-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was just tossing around a few thoughts, and I was was wondering if } anyone has experience cooling two instruments with one chiller. My } thinking is that you should be able to daisy-chain the diffusion pumps } together and achieve proper chilling, but the trade-off would be a } decrease in the required temperature coming out of the chiller. Does } the flow rate have to be augmented somehow as well? } } Perhaps the better solution is to put a Y connector in the chiller } lines and run the two in parallel? } } --Justin. } } -- } "America believes in education; the average professor earns more money } in a year than a professional athlete earns in a whole week." Evan } Esar } } ==============================Original Headers============================== } 4, 31 -- From kraftpiano-at-gmail.com Tue Feb 10 18:31:30 2009 } 4, 31 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.249]) } 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B0VTJp029396 } 4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 18:31:30 -0600 } 4, 31 -- Received: by rv-out-0708.google.com with SMTP id k29so86197rvb.30 } 4, 31 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 16:31:29 -0800 (PST) } 4, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 31 -- d=gmail.com; s=gamma; } 4, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject } 4, 31 -- :from:to:content-type:content-transfer-encoding; } 4, 31 -- bh=wKBgQ589KW6IQrXye9biIk/WzhipxgGu8sfjdx0bmWY=; } 4, 31 -- b=hsp0HV96GZB3nvJ7YkW+rdivO4kcOyywL4lxN44p4VeLazBNFdAx87cTMDVHMfehRj } 4, 31 -- vsFAWun8ORtL6LAXG5uDET57r6zJiM3nRPT7Msk5CNT2EIQQh6u6nHhUa69Z+lL59xzG } 4, 31 -- QOGf1J6ea0UWea673IAF1YRxnqFZTAddrmoTY= } 4, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 31 -- d=gmail.com; s=gamma; } 4, 31 -- h=mime-version:date:message-id:subject:from:to:content-type } 4, 31 -- :content-transfer-encoding; } 4, 31 -- b=igGn5V5s+hHIwnY353+jrgHHVWM7ppUa1nocNAkJQ4UwoQEZLQsF7CH4dJt6+qIfhe } 4, 31 -- CTaRtL5U4bA/eubxrKlm80HfXukEm0I0iFzGRoUuzE1x4iRSFqt2+JClg9vOBG/ZoTBr } 4, 31 -- aT+95zDnyoTP2Rl5+Zr6mTzz023V070yxKrCc= } 4, 31 -- MIME-Version: 1.0 } 4, 31 -- Received: by 10.141.100.15 with SMTP id c15mr3519rvm.222.1234312289521; Tue, } 4, 31 -- 10 Feb 2009 16:31:29 -0800 (PST) } 4, 31 -- Date: Tue, 10 Feb 2009 19:31:29 -0500 } 4, 31 -- Message-ID: {25e2b0d20902101631y3200acd6lf0389f51ef9a2499-at-mail.gmail.com} } 4, 31 -- Subject: SEM Cooling question. } 4, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com} } 4, 31 -- To: microscopy-at-microscopy.com } 4, 31 -- Content-Type: text/plain; charset=ISO-8859-1 } 4, 31 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at one. Lately it doesn't seem to be working."
==============================Original Headers============================== 7, 26 -- From colijn.1-at-osu.edu Tue Feb 10 20:03:04 2009 7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1B2343N007544 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 20:03:04 -0600 7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 7, 26 -- id {01N5CIUF1V1C8WX0KW-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 7, 26 -- Tue, 10 Feb 2009 21:03:03 -0500 (EST) 7, 26 -- Received: from [192.168.1.104] 7, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 7, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 7, 26 -- with ESMTPA id {01N5CIU8NH388WWFKH-at-ecr6.ohio-state.edu} for 7, 26 -- microscopy-at-microscopy.com; Tue, 10 Feb 2009 21:02:58 -0500 (EST) 7, 26 -- Date: Tue, 10 Feb 2009 21:02:54 -0500 7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 26 -- Subject: Re: [Microscopy] SEM Cooling question. 7, 26 -- In-reply-to: {200902110032.n1B0Wxg7031615-at-ns.microscopy.com} 7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- Message-id: {499231CE.8040208-at-osu.edu} 7, 26 -- MIME-version: 1.0 7, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-transfer-encoding: 7bit 7, 26 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 7, 26 -- References: {200902110032.n1B0Wxg7031615-at-ns.microscopy.com} ==============================End of - Headers==============================
Please note this two week course being held in Scandinavia at the end of June, beginning of July 2009. It is open to anyone with a background in TEM and/or STEM.
Leica-Microsystems is sponsoring a “Cryo Sample Preparation and Correlative Light and Electron Microscopy Techniques†workshop at the University of Maryland, Baltimore on March 16th & 17th, 2009.
The purpose of this workshop is to discuss the rapidly evolving new techniques for high pressure freezing, freeze substitution and cryo-sectioning. The use of correlative LM and EM techniques will be shown via a “live†video format. There will be a special emphasis on step by step procedures. Topics of discussion will include:
-How to optimize freezing for a wide variety of samples -Strategies for freeze substitution -How to cut cryo sections of frozen hydrated and sucrose infiltrated samples -Live cell imaging -Tomography
Individuals participating in this session will leave with a working knowledge of these cryo-techniques that they can apply immediately to their own research, whether it involves tomography, cryo-sectioning, EM immuno-labeling, or if they just want to have the best available preservation of cellular fine structure.
It is possible for registered users to try out the equipment with their own samples. Please go to our website (http://www.dental.umaryland.edu/Core-imaging/EMCF%20events/cryo-workshop/Workshop%20announcement ) for the tentative program and registration information.
Participation is FREE for all registered users. Register ASAP (http://www.dental.umaryland.edu/Core-imaging/EMCF%20events/cryo-workshop/Workshop%20announcement ).
We hope to see you there.
Best regards,
Ru-ching  Ru-ching Hsia, Ph.D å¤å¦‚è Associate Professor Director, Core Imaging Facility http://www.dental.umaryland.edu/Core-imaging Department of Microbial Pathogenesis University of Maryland Dental School Rm 9202, 650 West Baltimore Street Baltimore, MD 21201 Tel: 410-706 7992 Fax:: 410-706 0193 E-mail: rhsia-at-umaryland.edu
==============================Original Headers============================== 15, 20 -- From RHsia-at-umaryland.edu Wed Feb 11 07:20:33 2009 15, 20 -- Received: from cits-exch1.campus.umaryland.edu (cits-exch1.campus.umaryland.edu [134.192.1.123]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BDKWMn028889 15, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 07:20:33 -0600 15, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 20 -- Content-class: urn:content-classes:message 15, 20 -- MIME-Version: 1.0 15, 20 -- Content-Type: text/plain; 15, 20 -- charset="utf-8" 15, 20 -- Subject: cryo sample preparation and correlative LM & EM techniques workshop 15, 20 -- Date: Wed, 11 Feb 2009 08:20:30 -0500 15, 20 -- Message-ID: {3BBD3EDE51DB2043ABF3E819B1CD532A08B4A939-at-cits-exch1.campus.umaryland.edu} 15, 20 -- X-MS-Has-Attach: 15, 20 -- X-MS-TNEF-Correlator: 15, 20 -- Thread-Topic: cryo sample preparation and correlative LM & EM techniques workshop 15, 20 -- thread-index: AcmMS4GQlva0cVrZSKibsXyLdmQlhg== 15, 20 -- From: "Hsia, Ru-Ching" {RHsia-at-umaryland.edu} 15, 20 -- To: {Microscopy-at-microscopy.com} 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n1BDKWMn028889 ==============================End of - Headers==============================
PLEASE DO NOT REPLY TO THIS EMAIL; SEE CONTACT INFORMATION BELOW. Electron Microscopy Technician Position Available
Location: Duke University Medical Center, Durham, NC
Requirements: BS degree. US citizen or green card. Training and experience in running electron microscopes, proficiency in cutting ultrathin sections and performing negative staining. Knowledge of scientific laboratory operation (making solutions, ordering, typing results, keeping records, etc.). Clinical laboratory and research experience are a plus.
Laboratory description: The work force consists of the director and 6 EM technologists who perform pathology (500 samples/year), virology (1000 samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes—2 with cryo attachments, plus ancillary specimen preparation equipment.
EM Laboratory web site: http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain
Send resume to: Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Phone: 919 684-3452 Fax: 919 684-3265 Email: saram-at-duke.edu
==============================Original Headers============================== 9, 28 -- From hale0007-at-mc.duke.edu Wed Feb 11 09:58:07 2009 9, 28 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BFw5dt019310 9, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 09:58:07 -0600 9, 28 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54]) 9, 28 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n1BFw0Ek2584674 9, 28 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO) 9, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 10:58:00 -0500 9, 28 -- Importance: Normal 9, 28 -- X-Priority: 3 (Normal) 9, 28 -- In-Reply-To: 9, 28 -- References: 9, 28 -- Subject: EM tech position available 9, 28 -- MIME-Version: 1.0 9, 28 -- From: Michael J Hale {hale0007-at-mc.duke.edu} 9, 28 -- To: Microscopy-at-microscopy.com 9, 28 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release 9, 28 -- 8.0.2|August 07, 2008) at 02/11/2009 10:57:51, 9, 28 -- MIME-CD complete at 02/11/2009 10:57:51, 9, 28 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release 9, 28 -- 8.0.2|August 07, 2008) at 02/11/2009 10:57:59 AM 9, 28 -- Message-ID: {OF57FD88DC.E84078B3-ON8525755A.0057B1B7-8525755A.0057B1D1-at-notes.duke.edu} 9, 28 -- Date: Wed, 11 Feb 2009 10:57:51 -0500 9, 28 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008 9, 28 -- Content-type: text/plain; charset=UTF-8 9, 28 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n1BFw5dt019310 ==============================End of - Headers==============================
Dear All, first to summarise the thread on electron diffraction - although I didn't get many replies, I think Lew Rabenberg's reply is an excellent essay on the subject and summarises the current state of play very well indeed. Thank you Lew, and Bill Tivol, for your responses.
And now another question. Does anyone have an estimate for how many active TEM labs there are worldwide (covering all subject areas)? My guess is 'a few thousand'.. but perhaps someone has a better estimate?
Many thanks
Richard Beanland
==============================Original Headers============================== 5, 28 -- From contact-at-integrityscientific.com Wed Feb 11 11:12:24 2009 5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk (mail-relay-3.csv.warwick.ac.uk [137.205.128.9]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BHCLS8003869 5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 11:12:23 -0600 5, 28 -- Received: from localhost (localhost [127.0.0.1]) 5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHCELS008044 5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:14 GMT 5, 28 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk ([127.0.0.1]) 5, 28 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 5, 28 -- with LMTP id TLF9uQ5mqozL for {microscopy-at-microscopy.com} ; 5, 28 -- Wed, 11 Feb 2009 17:12:08 +0000 (GMT) 5, 28 -- Received: from [137.205.164.175] (hosts-137-205-164-175 [137.205.164.175]) 5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHC8xu007968 5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:08 GMT 5, 28 -- X-Envelope-From: contact-at-integrityscientific.com 5, 28 -- Message-ID: {499306E4.1050502-at-integrityscientific.com} 5, 28 -- Date: Wed, 11 Feb 2009 17:12:04 +0000 5, 28 -- From: Richard Beanland {contact-at-integrityscientific.com} 5, 28 -- Reply-To: contact-at-integrityscientific.com 5, 28 -- Organization: Integrity Scientific Ltd 5, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 5, 28 -- MIME-Version: 1.0 5, 28 -- To: microscopy-at-microscopy.com 5, 28 -- Subject: How many TEM labs are there worldwide? 5, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit 5, 28 -- X-DCC-Warwick-Metrics: anemone; whitelist ==============================End of - Headers==============================
Richard, I think your estimate is low. The University of Michigan of Ann Arbor has at least 4, probably more EM facilities. The TEM specification narrows it down a little, but we have one Anatomy and Cell biology teaching EM lab, a pay-per use research lab, a Biology EM lab, and a Geology EM lab. Now add all the hospitals, pharmaceutical companies, and some biotech startups and you have quite a list. I'm not even partially qualified to estimate materials EM labs.
How do you want to define "EM lab"? Would larger universities count as one lab, or several?
An EM sales rep might have some better numbers than our guesses, but I'm glad you've brought it up, since I'd like to know what you discover.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: contact-at-integrityscientific.com [mailto:contact-at-integrityscientific.com] Sent: Wednesday, February 11, 2009 12:27 PM To: Sobocinski, Gregg
Dear All, first to summarise the thread on electron diffraction - although I didn't get many replies, I think Lew Rabenberg's reply is an excellent essay on the subject and summarises the current state of play very well indeed. Thank you Lew, and Bill Tivol, for your responses.
And now another question. Does anyone have an estimate for how many active TEM labs there are worldwide (covering all subject areas)? My guess is 'a few thousand'.. but perhaps someone has a better estimate?
Many thanks
Richard Beanland
==============================Original Headers============================== 5, 28 -- From contact-at-integrityscientific.com Wed Feb 11 11:12:24 2009 5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk (mail-relay-3.csv.warwick.ac.uk [137.205.128.9]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BHCLS8003869 5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 11:12:23 -0600 5, 28 -- Received: from localhost (localhost [127.0.0.1]) 5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHCELS008044 5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:14 GMT 5, 28 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 5, 28 -- Received: from mail-relay-3.csv.warwick.ac.uk ([127.0.0.1]) 5, 28 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 5, 28 -- with LMTP id TLF9uQ5mqozL for {microscopy-at-microscopy.com} ; 5, 28 -- Wed, 11 Feb 2009 17:12:08 +0000 (GMT) 5, 28 -- Received: from [137.205.164.175] (hosts-137-205-164-175 [137.205.164.175]) 5, 28 -- by mail-relay-3.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n1BHC8xu007968 5, 28 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 17:12:08 GMT 5, 28 -- X-Envelope-From: contact-at-integrityscientific.com 5, 28 -- Message-ID: {499306E4.1050502-at-integrityscientific.com} 5, 28 -- Date: Wed, 11 Feb 2009 17:12:04 +0000 5, 28 -- From: Richard Beanland {contact-at-integrityscientific.com} 5, 28 -- Reply-To: contact-at-integrityscientific.com 5, 28 -- Organization: Integrity Scientific Ltd 5, 28 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 5, 28 -- MIME-Version: 1.0 5, 28 -- To: microscopy-at-microscopy.com 5, 28 -- Subject: How many TEM labs are there worldwide? 5, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit 5, 28 -- X-DCC-Warwick-Metrics: anemone; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 14, 25 -- From greggps-at-umich.edu Wed Feb 11 12:25:29 2009 14, 25 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BIPSHe020393 14, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 12:25:28 -0600 14, 25 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by 14, 25 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Wed, 11 Feb 14, 25 -- 2009 13:25:25 -0500 14, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 14, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 25 -- Date: Wed, 11 Feb 2009 13:25:24 -0500 14, 25 -- Subject: RE: [Microscopy] How many TEM labs are there worldwide? 14, 25 -- Thread-Topic: [Microscopy] How many TEM labs are there worldwide? 14, 25 -- Thread-Index: AcmMbf+uaB3m491ZTKiEUSK2B2IllgABpXQg 14, 25 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA3934018D7625-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} 14, 25 -- References: {200902111727.n1BHREdk016647-at-ns.microscopy.com} 14, 25 -- In-Reply-To: {200902111727.n1BHREdk016647-at-ns.microscopy.com} 14, 25 -- Accept-Language: en-US 14, 25 -- Content-Language: en-US 14, 25 -- X-MS-Has-Attach: 14, 25 -- X-MS-TNEF-Correlator: 14, 25 -- acceptlanguage: en-US 14, 25 -- Content-Type: text/plain; charset="us-ascii" 14, 25 -- MIME-Version: 1.0 14, 25 -- Content-Transfer-Encoding: 8bit 14, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1BIPSHe020393 ==============================End of - Headers==============================
This would probably give you a place to start - if you care to lay out a few thousand dollars. Or place a phone call and speak very very nicely to someone there!
PI } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Peter Ingram Duke University Medical Center Box 90319 LaSalle Street Extension DURHAM NC USA 27708-0319
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both JJasso1-at-neo.rr.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: JJasso1-at-neo.rr.com Name: Jerry Jasso
Title-Subject: [Filtered] MSA Local Affiliate Contact Information
Question: Dear All,
I am trying to reach the membership committee for Capital District Microscopy & Microanalysis Society to join this local affiliate. Unfortunately, the president listed at the MSA website cannot be reached. Does anyone have this contact information?
Asylum Research is seeking an Applications Scientist to assist current and potential customers in the use of its Atomic Force Microscopes (AFM) in the Oxford, UK office. Tasks will include performing demonstrations of the microscopes based in the Oxford laboratory, provision of technical support both in the field and office, and presentation of technical material at customer sites and conferences/ trade shows. The Applications Scientist will also be responsible for customer equipment installations and assisting with new product development and applications as dictated by customer requirements. Travel will be about 20%.
The successful candidate will have a solid academic background (PhD preferred) and will have a minimum of five years practical and theoretical AFM experience, preferably encompassing work in the fields of physics, materials, and/or life sciences. The candidate must have excellent communications skills, both written and oral, and must be able to understand customer needs, gain customer feedback and develop a strong understanding of competitive technology and products.
Asylum Research provides a competitive salary and comprehensive benefits. Interested candidates should email their resume to jobs-at-AsylumResearch.com . Please include the job title “Applications Scientist UK” in the subject line in the email along with salary requirements and references.
==============================Original Headers============================== 3, 14 -- From terry-at-AsylumResearch.com Wed Feb 11 14:49:42 2009 3, 14 -- Received: from exchange.AsylumResearch.com (exchange.asylumresearch.com [207.154.79.129]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BKnexe003079 3, 14 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 14:49:41 -0600 3, 14 -- From: Terry Mehr {terry-at-AsylumResearch.com} 3, 14 -- To: Microscopy-at-microscopy.com 3, 14 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 3, 14 -- Mime-Version: 1.0 (Apple Message framework v930.3) 3, 14 -- Subject: AFM Applications Scientist Job Opening in the UK 3, 14 -- Date: Wed, 11 Feb 2009 12:49:37 -0800 3, 14 -- X-Mailer: Apple Mail (2.930.3) 3, 14 -- Message-ID: {1LXM1a-0007H4-Iw-at-exchange.AsylumResearch.com} 3, 14 -- Content-Transfer-Encoding: 8bit 3, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1BKnexe003079 ==============================End of - Headers==============================
The Capital District Microscopy & Microanalysis Society, located in the Albany, NY area, is alive and well. We have just relocated and re-established our website (www.cdmms.org). Info about the Society, how to join, and who to contact can be found on the web page. We will get the information updated on the MSA and MAS (Microbeam Analysis Society) websites shortly. We welcome all within reach of our venues (and any beyond as well) to participate/join.
Thanks.
Jim McGee, President-elect, CDMMS
************************************ James J. McGee BMPC- KAPL Mail Bin 149 PO Box 1072 Schenectady, NY 12301-1072
-----Original Message----- X-from: JJasso1-at-neo.rr.com [mailto:JJasso1-at-neo.rr.com] Sent: Wednesday, February 11, 2009 3:17 PM To: McGee, James
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both JJasso1-at-neo.rr.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: JJasso1-at-neo.rr.com Name: Jerry Jasso
Title-Subject: [Filtered] MSA Local Affiliate Contact Information
Question: Dear All,
I am trying to reach the membership committee for Capital District Microscopy & Microanalysis Society to join this local affiliate. Unfortunately, the president listed at the MSA website cannot be reached. Does anyone have this contact information?
==============================Original Headers============================== 7, 15 -- From zaluzec-at-microscopy.com Wed Feb 11 14:05:20 2009 7, 15 -- Received: from vbn.inter-touch.net ([203.35.254.66]) 7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BK5Ijg020054 7, 15 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 14:05:19 -0600 7, 15 -- Received: from [10.5.0.10] (unknown [10.5.0.10]) 7, 15 -- by vbn.inter-touch.net (Postfix) with ESMTP id 8146D23194 7, 15 -- for {microscopy-at-microscopy.com} ; Thu, 12 Feb 2009 06:35:15 +1030 (CST) 7, 15 -- Mime-Version: 1.0 7, 15 -- Message-Id: {p06240806c5b8dfea8ed2-at-[10.5.0.10]} 7, 15 -- Date: Thu, 12 Feb 2009 06:35:13 +1030 7, 15 -- To: microscopy-at-microscopy.com 7, 15 -- From: JJasso1-at-neo.rr.com (by way of MicroscopyListserver) 7, 15 -- Subject: [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate 7, 15 -- Contact Information 7, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 17, 29 -- From mcgeejj-at-kapl.gov Wed Feb 11 15:23:22 2009 17, 29 -- Received: from ibetpms06.bias2000.bettis.gov (bettis.gov [65.170.176.212]) 17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BLNJ7O018516 17, 29 -- for {microscopy-at-microscopy.com} ; Wed, 11 Feb 2009 15:23:21 -0600 17, 29 -- Received: from atlas.bias2000.bettis.gov (unverified) by ibetpms06.bias2000.bettis.gov 17, 29 -- (Clearswift SMTPRS 5.2.9) with SMTP id {T8c728f068aac1002861dc4-at-ibetpms06.bias2000.bettis.gov} ; 17, 29 -- Wed, 11 Feb 2009 16:23:17 -0500 17, 29 -- Received: from ibetpex02.bias2000.bettis.gov ([172.16.2.129]) by atlas.bias2000.bettis.gov with Microsoft SMTPSVC(6.0.3790.1830); 17, 29 -- Wed, 11 Feb 2009 16:23:16 -0500 17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 29 -- Content-class: urn:content-classes:message 17, 29 -- MIME-Version: 1.0 17, 29 -- Content-Type: text/plain; 17, 29 -- charset="us-ascii" 17, 29 -- Subject: RE: [Microscopy] [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate 17, 29 -- Date: Wed, 11 Feb 2009 16:23:16 -0500 17, 29 -- Message-ID: {8807BA5B50FE0E40957843C1A36ACB8301E6C06C-at-ibetpex02.bias2000.bettis.gov} 17, 29 -- In-Reply-To: {200902112016.n1BKGlk3029298-at-ns.microscopy.com} 17, 29 -- X-MS-Has-Attach: 17, 29 -- X-MS-TNEF-Correlator: 17, 29 -- Thread-Topic: [Microscopy] [Filtered] MicroscopyListserverviaWWW: MSA Local Affiliate 17, 29 -- Thread-Index: AcmMhdPLzHO37QXfSIyYE5AXnbvzcAACCEcg 17, 29 -- References: {200902112016.n1BKGlk3029298-at-ns.microscopy.com} 17, 29 -- From: "McGee, James" {mcgeejj-at-kapl.gov} 17, 29 -- To: {microscopy-at-microscopy.com} 17, 29 -- Cc: {JJasso1-at-neo.rr.com} 17, 29 -- X-OriginalArrivalTime: 11 Feb 2009 21:23:16.0976 (UTC) FILETIME=[F45D2700:01C98C8E] 17, 29 -- Content-Transfer-Encoding: 8bit 17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1BLNJ7O018516 ==============================End of - Headers==============================
From mailbases-at-mail.ru Wed Feb 11 16:54:56 2009 Return-Path: {mailbases-at-mail.ru} Received: from google.com ([41.250.228.216]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1BMso0n002960 for {microscopylistserverarchive-at-microscopy.com} ; Wed, 11 Feb 2009 16:54:55 -0600 Received: from [162.61.56.83] (HELO google.com) by bright-hombre.org; Wed, 11 Feb 2009 22:54:49 +0000
Greetings,
To all students (or those of you who might have students) attending the M&M 2009 meeting in Richmond, please consider the student bursary program offered by MSA. The purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where they can meet and interact with the established microscopy community while defraying some meeting costs.
The students work for 20 hours (or up to 40 hours) during the meeting and pre-meeting events and are paid $10 an hour. The jobs involve such things as providing support in the different symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth or volunteer office, monitoring use of the Internet Café, and helping with poster set-up and take-down.
Once the final program has been established, each bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up *working* sessions they would attend anyway. There is an added bonus of a $10 cash meal allotment for each morning and/or afternoon sessions worked.
If anyone would like to participate in the bursary program, please check the *I wish to apply for a student bursary* box in section 2 of the registration form. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, and enrolled as students at a recognized educational institution. Don*t forget to check the MSA website for special discounted hotel rates especially for students as well as other scholarships to help defray even more meeting costs.
For those *non-students* we could always use volunteers to help with the above mentioned meeting activities as well. Although not paid on an hourly basis as the student bursaries, volunteers do receive some compensation along with the same cash allotment for meals. Plus they also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me.
Amanda Lawrence Electron Microscope Center Mississippi State University 662-325-3019 alawrence-at-entomology.msstate.edu
==============================Original Headers============================== 11, 23 -- From ALawrence-at-entomology.msstate.edu Fri Feb 13 07:46:02 2009 11, 23 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1DDk1gM001733 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 07:46:01 -0600 11, 23 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 11, 23 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n1DDk0Ku023002 11, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Feb 2009 07:46:00 -0600 11, 23 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 11, 23 -- with Novell_GroupWise; Fri, 13 Feb 2009 07:46:00 -0600 11, 23 -- Message-Id: {49952532.B26A.00E6.0-at-entomology.msstate.edu} 11, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 11, 23 -- Date: Fri, 13 Feb 2009 07:45:54 -0600 11, 23 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- Subject: M&M 2009 5 months and counting 11, 23 -- References: {49951C86020000E600057275-at-mailhost2.groupwise.msstate.edu} 11, 23 -- {49951C96020000E600057278-at-mailhost2.groupwise.msstate.edu} 11, 23 -- {49952532020000E60005728C-at-mailhost2.groupwise.msstate.edu} 11, 23 -- Mime-Version: 1.0 11, 23 -- Content-Type: text/plain; charset=ISO-8859-15 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- Content-Disposition: inline ==============================End of - Headers==============================
In terms of the operation of the optical microscope so much is on-line is these days, see our web-links: http://www.well.ox.ac.uk/cytogenetics/websites.shtml
Not sure what type of books you mean, but for fun coffee table reading [in a school sixth form or path lab anyway] try
Inside the body - Fantastic images from beneath the skin by Susan Greenfield (Foreword)
Unseen companions - Big views of tiny creature by Adrian Warren, David Spears, and Madeleine Spears
I use these occasionally for schools outreach programs.
Plus there's the microscope to telescope images from:
Heaven and Earth: Unseen by the Naked Eye (Photography)
Microcosmos: Discovering the World Through Microscopic Images from 40x to 100,000x Magnification
But these above two books are a bit thick with tiddly photo-sizes for the superb images contained.
Our web-site has a few 'serious' book suggestions but these are all for light microscopy [as that's what we do]. http://www.well.ox.ac.uk/cytogenetics/reading.shtml
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: MSHERWOOD-at-PARTNERS.ORG [mailto:MSHERWOOD-at-PARTNERS.ORG] Sent: 10 February 2009 19:48 To: kjmorris-at-well.ox.ac.uk
We are looking to purchase some good microscopy reference books or atlases. I know this topic has come up before, but I would like to know what people recommend.
Thanks! Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
==============================Original Headers============================== 9, 26 -- From MSHERWOOD-at-PARTNERS.ORG Tue Feb 10 13:38:54 2009 9, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1AJcsPA030977 9, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Feb 2009 13:38:54 -0600 9, 26 -- X-IronPort-AV: E=Sophos;i="4.38,187,1233550800"; 9, 26 -- d="scan'208";a="59281141" 9, 26 -- Received: from phsxcon1.mgh.harvard.edu (HELO PHSXCON1.partners.org) ([132.183.130.40]) 9, 26 -- by phsmgmx11.partners.org with ESMTP; 10 Feb 2009 14:38:54 -0500 9, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 9, 26 -- Tue, 10 Feb 2009 14:38:53 -0500 9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 26 -- Content-class: urn:content-classes:message 9, 26 -- MIME-Version: 1.0 9, 26 -- Subject: [Microscopy] Re: Microscopy Reference Books/Atlases 9, 26 -- Date: Tue, 10 Feb 2009 14:38:53 -0500 9, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9703E237F1-at-PHSXMB30.partners.org} 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- Thread-Topic: [Microscopy] Re: Microscopy Reference Books/Atlases 9, 26 -- Thread-Index: AcmLtzSGVT+XsttPShyD1IJxxj+s1w== 9, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 9, 26 -- To: {Microscopy-at-microscopy.com} 9, 26 -- X-OriginalArrivalTime: 10 Feb 2009 19:38:53.0927 (UTC) FILETIME=[34E1F770:01C98BB7] 9, 26 -- Content-Type: text/plain; charset="us-ascii" 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1AJcsPA030977 ==============================End of - Headers==============================
==============================Original Headers============================== 37, 22 -- From kjmorris-at-well.ox.ac.uk Fri Feb 13 10:43:04 2009 37, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 37, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1DGh3ZC023627 37, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Feb 2009 10:43:03 -0600 37, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 37, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 37, 22 -- id 1LY183-0001mZ-20 37, 22 -- for Microscopy-at-Microscopy.Com; Fri, 13 Feb 2009 16:43:03 +0000 37, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 37, 22 -- To: {Microscopy-at-Microscopy.Com} 37, 22 -- References: {200902101947.n1AJlorf019372-at-ns.microscopy.com} 37, 22 -- Subject: RE: [Microscopy] Re: Microscopy Reference Books/Atlases 37, 22 -- Date: Fri, 13 Feb 2009 16:43:02 -0000 37, 22 -- Message-ID: {A3C150EB9B6744019DB9AD177D8EE350-at-CytoWhizz} 37, 22 -- MIME-Version: 1.0 37, 22 -- Content-Type: text/plain; 37, 22 -- charset="us-ascii" 37, 22 -- Content-Transfer-Encoding: 7bit 37, 22 -- X-Mailer: Microsoft Office Outlook 11 37, 22 -- In-Reply-To: {200902101947.n1AJlorf019372-at-ns.microscopy.com} 37, 22 -- Thread-Index: AcmLuHUAwJOCNNBUQhqqqOk/YbZrZACMcWKw 37, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 ==============================End of - Headers==============================
Kraft Foods Global, Inc. is currently seeking Scientist-Physical Characterization, Analytical Sciences to work within our Tarrytown, NY facility.
Key responsibilities include the following: * Leverage a strong multidisciplinary scientific background and powder characterization expertise to support various R&D projects for a broad range of food systems * Apply current and develop new and innovative techniques to characterize and solve practical problems related to food powders * Develop a fundamental understanding of food powder properties to provide solutions in the areas of ingredients, formulation, processing, product quality and product performance: powder stability (e.g.: caking, sorption isotherms, glass transition), dispersion in liquids, flowability, segregation/demixing, dusting, process monitoring/control and mixing phenomena. * Develop and apply a broad physical characterization expertise * This position will be located in Tarrytown, NY and will require travel 1 day per week to our East Hanover, NJ facility. * Build strong working relationships with clients/customers and build projects and programs to serve their needs * Build, maintain and leverage strong internal and external network of technical experts and resources to speedily execute and serve project needs * Collaborate across R&D as a cross-functional team member and develop technical leadership * Communicate effectively technical findings to project team and management Qualified candidates should possess the following: * MS or PhD in Science or Engineering with a strong proven scientific and experimental background in powder characterization * 0-3 years of experience in powder characterization, including thermal analysis, powder flow and caking, powder surface chemistry and particle size & shape, with a passion to develop & apply scientific findings to solve practical problems * Demonstrated in-depth multidisciplinary scientific knowledge in two or more of the following areas: Physical Chemistry, Interfacial Colloid Science, Polymer Science & Process Engineering * Breadth and depth of scientific knowledge in physical characterization and the ability to develop new areas of expertise quickly and effectively * Ability to build and speedily invoke recent scientific advances and global network of experts and resources to rapidly advance projects * Strong and proven interpersonal skills, with the ability to build strong customer/client relationships * Ability to work together in cross-functional teams in various roles * Excellent project management, written and verbal communication skills
If you are interested in applying for this opportunity, please cut and paste the following link into your web browser to apply directly via the www.kraftfoods.com/careers web site:
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Email: wall1-at-llnl.gov Name: Mark Wall
Organization: Lawrence Livermore National Lab
Title-Subject: [Filtered] Image J pluins
Question: Is anyone willing to help me off-line on using Image plugins for making stacks and using other plugins, such as Z-stack and stack focuser. It appears that I get a Java errors when trying to us the stack focuser on a created stack.
This has been the year of challenging projects and the year is still young. Now another one has come over the transom.
One of our researchers is experimenting with photo-resist patterns on 6-inch silicon wafers. Our task is to image cross-sections of tiny little lines contained in rows of tiny little dots about the size of TEM grids. Imaging mags are around 100-200kX, so we're talking little here. Getting these wafers to break accurately is proving to be a real bear and it may be that the break is distorting the cross-sections.
I'm using a diamond scribe to score the wafers as accurately as I can, but scribing the back of the wafer to get an accurate break on the front is proving to be difficult.
There must be lots of industry folks out there who do this on a daily basis. Would someone be willing to share a few tiny little hints?
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 10, 27 -- From TindallR-at-missouri.edu Mon Feb 16 09:34:53 2009 10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GFYqGp008710 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 09:34:53 -0600 10, 27 -- X-IronPort-Anti-Spam-Filtered: true 10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAK8WmUnRauUp/2dsb2JhbADEGQEJhEOISoJngTUG 10, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Feb 2009 09:34:50 -0600 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 27 -- Mon, 16 Feb 2009 09:34:50 -0600 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 27 -- Content-class: urn:content-classes:message 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; 10, 27 -- charset="us-ascii" 10, 27 -- Subject: SEM: Breaking wafers accurately 10, 27 -- Date: Mon, 16 Feb 2009 09:34:50 -0600 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DF7-at-UM-XMAIL08.um.umsystem.edu} 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- Thread-Topic: SEM: Breaking wafers accurately 10, 27 -- Thread-Index: AcmQTBrd6/BRLzcJTrSkB7IWRcY2Ww== 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 27 -- To: {microscopy-at-microscopy.com} 10, 27 -- X-OriginalArrivalTime: 16 Feb 2009 15:34:50.0811 (UTC) FILETIME=[1B6248B0:01C9904C] 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1GFYqGp008710 ==============================End of - Headers==============================
I more than occasionally make cleaves through Si wafers, with several types of resist, with the intent of cross sectioning very small features for SEM. Typically these are scribed on the front side with a diamond scribe and cleaved with polymer glaziers-pliers. If need to hit a very small (sub-micron) feature exactly, I make a series of reductional cleaves winding up with a SELA microcleaver to get the final cross section. Resist is one of the harder samples from which to get a good, undistorted specimen. Then you need to get an undistorted image.... I don't cleave TEM samples.
Hope this helps,
John
} [Original Message] } From: {TindallR-at-missouri.edu} } To: {jwheckman-at-earthlink.net} } Date: 2/16/2009 8:41:57 AM } Subject: [Microscopy] SEM: Breaking wafers accurately } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Collective, } } This has been the year of challenging projects and the year is still } young. Now another one has come over the transom. } } One of our researchers is experimenting with photo-resist patterns on } 6-inch silicon wafers. Our task is to image cross-sections of tiny } little lines contained in rows of tiny little dots about the size of TEM } grids. Imaging mags are around 100-200kX, so we're talking little here. } Getting these wafers to break accurately is proving to be a real bear } and it may be that the break is distorting the cross-sections. } } I'm using a diamond scribe to score the wafers as accurately as I can, } but scribing the back of the wafer to get an accurate break on the front } is proving to be difficult. } } There must be lots of industry folks out there who do this on a daily } basis. Would someone be willing to share a few tiny little hints? } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } } ==============================Original Headers============================== } 10, 27 -- From TindallR-at-missouri.edu Mon Feb 16 09:34:53 2009 } 10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GFYqGp008710 } 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 09:34:53 -0600 } 10, 27 -- X-IronPort-Anti-Spam-Filtered: true } 10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAK8WmUnRauUp/2dsb2JhbADEGQEJhEOISoJngTUG } 10, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) } 10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Feb 2009 09:34:50 -0600 } 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 10, 27 -- Mon, 16 Feb 2009 09:34:50 -0600 } 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 10, 27 -- Content-class: urn:content-classes:message } 10, 27 -- MIME-Version: 1.0 } 10, 27 -- Content-Type: text/plain; } 10, 27 -- charset="us-ascii" } 10, 27 -- Subject: SEM: Breaking wafers accurately } 10, 27 -- Date: Mon, 16 Feb 2009 09:34:50 -0600 } 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DF7-at-UM-XMAIL08.um.umsystem.edu} } 10, 27 -- X-MS-Has-Attach: } 10, 27 -- X-MS-TNEF-Correlator: } 10, 27 -- Thread-Topic: SEM: Breaking wafers accurately } 10, 27 -- Thread-Index: AcmQTBrd6/BRLzcJTrSkB7IWRcY2Ww== } 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 10, 27 -- To: {microscopy-at-microscopy.com} } 10, 27 -- X-OriginalArrivalTime: 16 Feb 2009 15:34:50.0811 (UTC) FILETIME=[1B6248B0:01C9904C] } 10, 27 -- Content-Transfer-Encoding: 8bit } 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1GFYqGp008710 } ==============================End of - Headers==============================
Some of our SAD diffraction patterns are showing rings that are discontinuous. Only a small segment of the rings are sharp (say, a wedge comprising 25% of the circle) and they taper off to nothingness, so that directly opposite the sharpest rings, we see nothing at all. I was wondering what might be causing this (uneven specimen thickness, specimen tilted, illumination misalignment, astigmatism). When we diffract a gold standard, no problem, so I conclude that it must be specimen related.
Any ideas or suggestions to help us solve this SAD situation (sorry, I couldn't resist....)? -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: jtugglenator-at-gmail.com Name: Jay Tuggle
Title-Subject: [Filtered] TEM on Antibodies
Question: Wondered if anyone had a fairly simple and effective method for looking a antibodies on the TEM. Most of the sources i have are pretty old and i think out dated.
Bacteria can be difficult to infiltrate with resin. Even using Spurr's, and increased infiltration times, I often end up with holes in the center of some of the bacteria. Are there any good tricks for improving the infiltration of bacteria?
Ralph Common Dept. of Physiology Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Mon Feb 16 14:08:19 2009 4, 24 -- Received: from sys52.mail.msu.edu (sys52.mail.msu.edu [35.9.75.232]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GK8Jlo009180 4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 14:08:19 -0600 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys52.mail.msu.edu with esmtpsa (Exim 4.63 #12) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1LZ9lK-0004y0-Tg 4, 24 -- for Microscopy-at-microscopy.com; Mon, 16 Feb 2009 15:08:18 -0500 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: TEM of bacteria 4, 24 -- Date: Mon, 16 Feb 2009 15:08:18 -0500 4, 24 -- Message-ID: {001801c99072$4f829840$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- Importance: Normal 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Dear John, The SAD is showing you the crystallinity of your sample, where a single, large selected crystal will give you a regular spot pattern, a large number of selected crystals will give you sharp rings, like in your gold standard, and diffuse light with no pattern suggests an amorphous material. You would get half a ring pattern if your SAD aperture was on the edge of the sample, the other half of the pattern would be black. You might also get half a pattern if your aperture was on the thin edge of a thick particle, where the beam could not penetrate the thicker part. You also might get half a pattern if your SAD aperture was bridging a crystalline and amorphous area of your sample or sampling an amorphous oxide layer. Does the pattern change when you move about the sample? Is the part with no pattern diffuse white or black? I always go back to the image to see what I am selecting, when I get a result I don't expect. Regards,
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: February 16, 2009 11:39 AM To: maryflet-at-interchange.ubc.ca
Some of our SAD diffraction patterns are showing rings that are discontinuous. Only a small segment of the rings are sharp (say, a wedge comprising 25% of the circle) and they taper off to nothingness, so that directly opposite the sharpest rings, we see nothing at all. I was wondering what might be causing this (uneven specimen thickness, specimen tilted, illumination misalignment, astigmatism). When we diffract a gold standard, no problem, so I conclude that it must be specimen related.
Any ideas or suggestions to help us solve this SAD situation (sorry, I couldn't resist....)? -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
All of the silicon wafers I work with, and cleave into pieces, have preferred cleavage planes along the crystal lattice. The directions, and angles will depend on the type of wafer you have. The wafers I work with, which are for integrated circuit fabrication, have two very nice cleavage planes, at 90 degrees apart, which works nicely for cleaving cross sections of the circuitry. I can cleave a straight line across a 300mm (12 inch) wafer by making a little scratch, maybe 1/8 inch long, in line with the cleavage plane, on the edge of the wafer. I then lay the scribe down on the bench top, with about 1/4 inch of the approximately 1/8 inch diameter shaft, under the edge of the wafer, lined up with the scratch mark. Then I hold one side of the wafer down to the bench top, and gently press the other side down over the scribe shaft. The crack starts at the scratch, and goes across the wafer. Occasionally, it will pick up a curve along the way, but most of the time it is as straight as an arrow.
All bets are off, if you have amorphous wafers.
Hope this helped, Darrell
TindallR-at-missouri.edu wrote on 02/16/2009 10:36:41 AM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Collective, } } This has been the year of challenging projects and the year is still } young. Now another one has come over the transom. } } One of our researchers is experimenting with photo-resist patterns on } 6-inch silicon wafers. Our task is to image cross-sections of tiny } little lines contained in rows of tiny little dots about the size of TEM } grids. Imaging mags are around 100-200kX, so we're talking little here. } Getting these wafers to break accurately is proving to be a real bear } and it may be that the break is distorting the cross-sections. } } I'm using a diamond scribe to score the wafers as accurately as I can, } but scribing the back of the wafer to get an accurate break on the front } is proving to be difficult. } } There must be lots of industry folks out there who do this on a daily } basis. Would someone be willing to share a few tiny little hints? } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } } ==============================Original Headers============================== } 10, 27 -- From TindallR-at-missouri.edu Mon Feb 16 09:34:53 2009 } 10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n1GFYqGp008710 } 10, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Feb 2009 09:34:53 -0600 } 10, 27 -- X-IronPort-Anti-Spam-Filtered: true } 10, 27 -- X-IronPort-Anti-Spam-Result: } ApoEAK8WmUnRauUp/2dsb2JhbADEGQEJhEOISoJngTUG } 10, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) } ([209.106.229.41]) } 10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 16 Feb } 2009 09:34:50 -0600 } 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228. } 34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 10, 27 -- Mon, 16 Feb 2009 09:34:50 -0600 } 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 10, 27 -- Content-class: urn:content-classes:message } 10, 27 -- MIME-Version: 1.0 } 10, 27 -- Content-Type: text/plain; } 10, 27 -- charset="us-ascii" } 10, 27 -- Subject: SEM: Breaking wafers accurately } 10, 27 -- Date: Mon, 16 Feb 2009 09:34:50 -0600 } 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7DF7-at-UM- } XMAIL08.um.umsystem.edu} } 10, 27 -- X-MS-Has-Attach: } 10, 27 -- X-MS-TNEF-Correlator: } 10, 27 -- Thread-Topic: SEM: Breaking wafers accurately } 10, 27 -- Thread-Index: AcmQTBrd6/BRLzcJTrSkB7IWRcY2Ww== } 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 10, 27 -- To: {microscopy-at-microscopy.com} } 10, 27 -- X-OriginalArrivalTime: 16 Feb 2009 15:34:50.0811 (UTC) } FILETIME=[1B6248B0:01C9904C] } 10, 27 -- Content-Transfer-Encoding: 8bit } 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns. } microscopy.com id n1GFYqGp008710 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 28 -- From milesd-at-us.ibm.com Mon Feb 16 17:31:37 2009 9, 28 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1GNVbRg014117 9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 17:31:37 -0600 9, 28 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 9, 28 -- by e6.ny.us.ibm.com (8.13.1/8.13.1) with ESMTP id n1GNWMUe005942 9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:32:22 -0500 9, 28 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 9, 28 -- by d01relay02.pok.ibm.com (8.13.8/8.13.8/NCO v9.1) with ESMTP id n1GNVa9K187620 9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:31:36 -0500 9, 28 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 9, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id n1GNVanW004499 9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:31:36 -0500 9, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 9, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id n1GNVaNR004491 9, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Feb 2009 18:31:36 -0500 9, 28 -- In-Reply-To: {200902161536.n1GFafH8009749-at-ns.microscopy.com} 9, 28 -- To: Microscopy-at-Microscopy.Com 9, 28 -- MIME-Version: 1.0 9, 28 -- Subject: Re: [Microscopy] SEM: Breaking wafers accurately 9, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 9, 28 -- Message-ID: {OF70376F20.871A81D0-ON8525755F.007E7D80-8525755F.0081356C-at-us.ibm.com} 9, 28 -- From: Darrell Miles {milesd-at-us.ibm.com} 9, 28 -- Date: Mon, 16 Feb 2009 18:31:38 -0500 9, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1 HF8|December 19, 2008) at 9, 28 -- 02/16/2009 18:31:40, 9, 28 -- Serialize complete at 02/16/2009 18:31:40 9, 28 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
} Ask the researcher, or if necessary the wafer manufacturer, to tell } you which crystal orientation is used in making the original wafers } before they are etched, then scribe along a line appropriate to } that angle. It makes a big difference; scribing and then breaking } along the wrong angle shatters the wafer. I used to scribe part-way } on the front of the wafer so that I could see where I was, and the } break would continue past the score to give nice cross-sections - } same idea as making glass knives for sectioning. } } Lesley Weston. } } } On 16-Feb-09, at 7:39 AM, TindallR-at-missouri.edu wrote: } } } } } } } } } --------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } Dear Collective, } } } } This has been the year of challenging projects and the year is still } } young. Now another one has come over the transom. } } } } One of our researchers is experimenting with photo-resist patterns on } } 6-inch silicon wafers. Our task is to image cross-sections of tiny } } little lines contained in rows of tiny little dots about the size } } of TEM } } grids. Imaging mags are around 100-200kX, so we're talking little } } here. } } Getting these wafers to break accurately is proving to be a real bear } } and it may be that the break is distorting the cross-sections. } } } } I'm using a diamond scribe to score the wafers as accurately as I } } can, } } but scribing the back of the wafer to get an accurate break on the } } front } } is proving to be difficult. } } } } There must be lots of industry folks out there who do this on a daily } } basis. Would someone be willing to share a few tiny little hints? } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl? } } Op=Splash&Amount= } } Week&NavType=Both&Type=TimePlan } } Sons of Norway: http://www.sofn.com } }
==============================Original Headers============================== 8, 22 -- From leswes-at-shaw.ca Tue Feb 17 09:55:18 2009 8, 22 -- Received: from idcmail-mo1so.shaw.ca (idcmail-mo1so.shaw.ca [24.71.223.10]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HFtGd4029183 8, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 09:55:17 -0600 8, 22 -- Received: from pd3ml2so-ssvc.prod.shaw.ca ([10.0.141.138]) 8, 22 -- by pd4mo1so-svcs.prod.shaw.ca with ESMTP; 17 Feb 2009 08:55:12 -0700 8, 22 -- X-Cloudmark-SP-Filtered: true 8, 22 -- X-Cloudmark-SP-Result: v=1.0 c=0 a=Zx37jsudAAAA:8 a=KKXDUkkEAAAA:8 a=T6abDhuPAAAA:8 a=fMNaZ91lPsFT39mxYYsA:9 a=92rRVBJz9-OWWpaKKowA:7 a=SSRIeVQFFbXejdZ12ejksDrAm-UA:4 a=aDP_jk9KaZEA:10 a=aw0-s5nGupUA:10 a=NROiimMUsE4A:10 a=KEUJ_xxvRqkA:10 a=RaRL5AVhiQEA:10 a=O27iSwohtSwA:10 a=njno5oAFA7sA:10 8, 22 -- Received: from unknown (HELO [96.49.149.2]) ([96.49.149.2]) 8, 22 -- by pd3ml2so-dmz.prod.shaw.ca with ESMTP; 17 Feb 2009 08:55:12 -0700 8, 22 -- In-Reply-To: {17F1115D-BE49-4B32-8FB4-AB871E6593C5-at-shaw.ca} 8, 22 -- References: {200902161539.n1GFdnLr012682-at-ns.microscopy.com} {17F1115D-BE49-4B32-8FB4-AB871E6593C5-at-shaw.ca} 8, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 22 -- Message-Id: {D3F433C8-4F7D-4F5B-9A2D-4D2BD2BE8A66-at-shaw.ca} 8, 22 -- Cc: Microscopy-at-microscopy.com 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- From: Lesley Weston {leswes-at-shaw.ca} 8, 22 -- Subject: Re: [Microscopy] SEM: Breaking wafers accurately 8, 22 -- Date: Tue, 17 Feb 2009 07:55:05 -0800 8, 22 -- To: Lesley Weston {leswes-at-shaw.ca} 8, 22 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
We want to be able to closely examine the fibers in cross-sections of various types and grades of paper.
The first question we need to answer is what material to embed with, and which type of microtome would be best to use to make the cross-sections? The second question is how important is sputter-coating and does anyone have a recommendation regarding the material to coat with? Lastly, to avoid re-inventing the wheel, does anyone have images of this type that they'd be willing to share?
Robert Zonis Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635 robert.zonis-at-sanford.com
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==============================Original Headers============================== 7, 31 -- From Robert.Zonis-at-Sanford.com Tue Feb 17 10:16:17 2009 7, 31 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGGEOp003353 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:16:16 -0600 7, 31 -- X-VirusChecked: Checked 7, 31 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 7, 31 -- X-Msg-Ref: server-10.tower-192.messagelabs.com!1234887369!28438663!2 7, 31 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 7, 31 -- X-Originating-IP: [198.176.16.25] 7, 31 -- Received: (qmail 11161 invoked from network); 17 Feb 2009 16:16:10 -0000 7, 31 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25) 7, 31 -- by server-10.tower-192.messagelabs.com with RC4-SHA encrypted SMTP; 17 Feb 2009 16:16:10 -0000 7, 31 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 7, 31 -- Tue, 17 Feb 2009 10:16:09 -0600 7, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 31 -- Content-class: urn:content-classes:message 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; 7, 31 -- charset="iso-8859-1" 7, 31 -- Subject: EM - Cross-sections of paper grades 7, 31 -- Date: Tue, 17 Feb 2009 10:13:30 -0600 7, 31 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401B6C726-at-naoaksasebe02.nr.ad.newellco.com} 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- Thread-Topic: EM - Cross-sections of paper grades 7, 31 -- Thread-Index: AcmRGqSgtbQ6aTrPT5mR+wwnF0kfiQ== 7, 31 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 7, 31 -- To: {Microscopy-at-microscopy.com} 7, 31 -- X-OriginalArrivalTime: 17 Feb 2009 16:16:09.0357 (UTC) FILETIME=[0B2023D0:01C9911B] 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HGGEOp003353 ==============================End of - Headers==============================
I think that earlier respondents have missed the point of your query. From my reading of your question, the answer is that you have a textured sample. With a polycrystalline sample you get uniform rings only if the grains are randomly oriented. If the grains have a preferred orientation then the rings will have intensity that varies round the diameter of each ring. See Hirsch et al 1965 or 1977 pages116-117; Williams and Carter 1996 pages 273-275 or Reimer 1984 pages 406-407
Good luck, Alwyn Eades
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 6, 21 -- From jae5-at-lehigh.edu Tue Feb 17 10:35:07 2009 6, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGZ5vV023318 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:06 -0600 6, 21 -- Received: from [127.0.0.1] (r054054.mat.Lehigh.EDU [128.180.54.54]) 6, 21 -- (authenticated bits=0) 6, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n1HGYZlb031426 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:35:03 -0500 6, 21 -- Message-ID: {499AE71F.8040104-at-lehigh.edu} 6, 21 -- Date: Tue, 17 Feb 2009 11:34:39 -0500 6, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 6, 21 -- Organization: Lehigh University 6, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 6, 21 -- MIME-Version: 1.0 6, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 6, 21 -- Subject: RE: TEM: diffraction pattern question 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on rain.cc.lehigh.edu 6, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Hello, I love being a biological service facility! I have a student from electrical engineering that has 'polymer' fibers that are water soluble and sensitive to temperatures below freezing. He wants TEM cross-sections of the fibers. 0_o
The gauntlet has been thrown, any ideas? Cryo's out due to temp issue, so can one section dry at room temp (provided the fibers don't dissolve in any resin)? Sorry I don't know the constitution of the fibers, just the few physical issues with them-they apper to be thinner than, but similar in appearence to, spiderweb... (I love challenges!)
Thanks in advance!
Tracey Pepper Microscopy and NanoImaging Facility Iowa State University Ames, IA 50011-1020 (515) 294-3872
==============================Original Headers============================== 6, 21 -- From tpepper-at-iastate.edu Tue Feb 17 10:35:21 2009 6, 21 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGZKEj023698 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:20 -0600 6, 21 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 6, 21 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n1HGZI0S017865 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:18 -0600 6, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 6, 21 -- id 5bfd_76ba80d0_fd0f_11dd_b413_001372578af6; 6, 21 -- Tue, 17 Feb 2009 10:24:34 -0600 6, 21 -- Received: from Debug (webmail-12.iastate.edu [129.186.140.32]) 6, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id n1HGZGrc024171 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:16 -0600 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu} 6, 21 -- Subject: A challenge 6, 21 -- Date: Tue, 17 Feb 2009 10:35:18 -0600 (CST) 6, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU Version mp9.11 6, 21 -- Message-Id: {1835101711092470-at-webmail.iastate.edu} 6, 21 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.17.162227 6, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0, FROM_EDU_TLD 0, __C230066_P5 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Both JEOL and Gatan show cross sections of paper in some of the product ads. Check their websites for info.
Roseann Csencsits, PhD Scientist in Charge - Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Feb 17, 2009, at 8:30 AM, Robert.Zonis-at-Sanford.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, all. } } We want to be able to closely examine the fibers in cross-sections } of various types and grades of paper. } } The first question we need to answer is what material to embed with, } and which type of microtome would be best to use to make the cross- } sections? The second question is how important is sputter-coating } and does anyone have a recommendation regarding the material to coat } with? Lastly, to avoid re-inventing the wheel, does anyone have } images of this type that they'd be willing to share? } } Robert Zonis } Sanford L.P. - A Newell Rubbermaid Company } Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } robert.zonis-at-sanford.com } } This message is intended for the Microscopy Listserv. Permission is } specifically granted to the Microscopy Society of America to publish } some or all of this message in the Microscopy Today journal. } } This message may contain information that is confidential and/or } protected by law. If the reader of this message is not the intended } recipient, you are hereby notified that any dissemination, } distribution, copying or communication of this message is strictly } prohibited. If you have received this communication in error, please } contact the sender immediately and delete the message. Please note } that although we will take all commercially reasonable efforts to } prevent viruses from being transmitted from our systems, it is the } responsibility of the recipient to check for and prevent adverse } action by viruses on its own systems. } } ______________________________________________________________________ } This email has been scanned by the MessageLabs Email Security System. } For more information please visit http://www.messagelabs.com/email } ______________________________________________________________________ } } } ==============================Original } Headers============================== } 7, 31 -- From Robert.Zonis-at-Sanford.com Tue Feb 17 10:16:17 2009 } 7, 31 -- Received: from mail192.messagelabs.com } (mail192.messagelabs.com [216.82.241.243]) } 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n1HGGEOp003353 } 7, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:16:16 } -0600 } 7, 31 -- X-VirusChecked: Checked } 7, 31 -- X-Env-Sender: Robert.Zonis-at-Sanford.com } 7, 31 -- X-Msg-Ref: server-10.tower-192.messagelabs.com!1234887369! } 28438663!2 } 7, 31 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- } 7, 31 -- X-Originating-IP: [198.176.16.25] } 7, 31 -- Received: (qmail 11161 invoked from network); 17 Feb 2009 } 16:16:10 -0000 } 7, 31 -- Received: from naehub2.newellco.com (HELO } naehub2.newellco.com) (198.176.16.25) } 7, 31 -- by server-10.tower-192.messagelabs.com with RC4-SHA } encrypted SMTP; 17 Feb 2009 16:16:10 -0000 } 7, 31 -- Received: from naoaksasebe02.nr.ad.newellco.com } ([10.217.158.64]) by naehub2.newellco.com with Microsoft } SMTPSVC(6.0.3790.1830); } 7, 31 -- Tue, 17 Feb 2009 10:16:09 -0600 } 7, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 7, 31 -- Content-class: urn:content-classes:message } 7, 31 -- MIME-Version: 1.0 } 7, 31 -- Content-Type: text/plain; } 7, 31 -- charset="iso-8859-1" } 7, 31 -- Subject: EM - Cross-sections of paper grades } 7, 31 -- Date: Tue, 17 Feb 2009 10:13:30 -0600 } 7, 31 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9401B6C726-at-naoaksasebe02.nr.ad.newellco.com } } } 7, 31 -- X-MS-Has-Attach: } 7, 31 -- X-MS-TNEF-Correlator: } 7, 31 -- Thread-Topic: EM - Cross-sections of paper grades } 7, 31 -- Thread-Index: AcmRGqSgtbQ6aTrPT5mR+wwnF0kfiQ== } 7, 31 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} } 7, 31 -- To: {Microscopy-at-microscopy.com} } 7, 31 -- X-OriginalArrivalTime: 17 Feb 2009 16:16:09.0357 (UTC) } FILETIME=[0B2023D0:01C9911B] } 7, 31 -- Content-Transfer-Encoding: 8bit } 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n1HGGEOp003353 } ==============================End of - } Headers==============================
==============================Original Headers============================== 13, 26 -- From RCsencsits-at-lbl.gov Tue Feb 17 10:48:44 2009 13, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGmg21021076 13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:48:43 -0600 13, 26 -- X-Ironport-SBRS: 2.3 13, 26 -- X-IronPort-Anti-Spam-Filtered: true 13, 26 -- X-IronPort-Anti-Spam-Result: AoEGAIx5mkmAAykYe2dsb2JhbACBSZMAAQEWIgVbrksJjw+CXoE1Bg 13, 26 -- X-IronPort-AV: E=Sophos;i="4.38,224,1233561600"; 13, 26 -- d="scan'208";a="117160986" 13, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 13, 26 -- by ironport1.lbl.gov with ESMTP; 17 Feb 2009 08:48:32 -0800 13, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246]) 13, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n1HGmVpn013486; 13, 26 -- Tue, 17 Feb 2009 08:48:31 -0800 (PST) 13, 26 -- Cc: Microscopy-at-microscopy.com 13, 26 -- Message-Id: {A5B7D447-1D60-4069-A996-FC53803C7890-at-lbl.gov} 13, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 13, 26 -- To: Robert.Zonis-at-Sanford.com 13, 26 -- In-Reply-To: {200902171630.n1HGURrM018899-at-ns.microscopy.com} 13, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 26 -- Content-Transfer-Encoding: 7bit 13, 26 -- Mime-Version: 1.0 (Apple Message framework v930.3) 13, 26 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades 13, 26 -- Date: Tue, 17 Feb 2009 08:48:28 -0800 13, 26 -- References: {200902171630.n1HGURrM018899-at-ns.microscopy.com} 13, 26 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Wow, that's a tough one. Ranks right up there with our guy who wants to see the size of water droplets in diesel fuel.
Anyway, I guess the first thing I'd try is to test the fibers in some resins to see if they stay together. If they don't dissolve, I'd try embedding them and dry-cutting rather large sections with a glass knife, then using a clamshell (hinged) grid to pop them into the TEM without the need to adhere them to anything.
Good luck!
Cheers, Randy
Electron Microscopy Core Staff W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 / 4777 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
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-----Original Message----- X-from: tpepper-at-iastate.edu [mailto:tpepper-at-iastate.edu] Sent: Tuesday, February 17, 2009 10:37 AM To: Tindall, Randy D.
Hello, I love being a biological service facility! I have a student from electrical engineering that has 'polymer' fibers that are water soluble and sensitive to temperatures below freezing. He wants TEM cross-sections of the fibers. 0_o
The gauntlet has been thrown, any ideas? Cryo's out due to temp issue, so can one section dry at room temp (provided the fibers don't dissolve in any resin)? Sorry I don't know the constitution of the fibers, just the few physical
issues with them-they apper to be thinner than, but similar in appearence to, spiderweb... (I love challenges!)
Thanks in advance!
Tracey Pepper Microscopy and NanoImaging Facility Iowa State University Ames, IA 50011-1020 (515) 294-3872
==============================Original Headers============================== 6, 21 -- From tpepper-at-iastate.edu Tue Feb 17 10:35:21 2009 6, 21 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGZKEj023698 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:20 -0600 6, 21 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 6, 21 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n1HGZI0S017865 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:18 -0600 6, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 6, 21 -- id 5bfd_76ba80d0_fd0f_11dd_b413_001372578af6; 6, 21 -- Tue, 17 Feb 2009 10:24:34 -0600 6, 21 -- Received: from Debug (webmail-12.iastate.edu [129.186.140.32]) 6, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id n1HGZGrc024171 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:16 -0600 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu} 6, 21 -- Subject: A challenge 6, 21 -- Date: Tue, 17 Feb 2009 10:35:18 -0600 (CST) 6, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU Version mp9.11 6, 21 -- Message-Id: {1835101711092470-at-webmail.iastate.edu} 6, 21 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.17.162227 6, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0, FROM_EDU_TLD 0, __C230066_P5 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __SANE_MSGID 0' ==============================End of - Headers==============================
==============================Original Headers============================== 21, 30 -- From TindallR-at-missouri.edu Tue Feb 17 10:58:54 2009 21, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGwqQ3002803 21, 30 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:58:53 -0600 21, 30 -- X-IronPort-Anti-Spam-Filtered: true 21, 30 -- X-IronPort-Anti-Spam-Result: ApoEABt8mknRauUo/2dsb2JhbADCToE+AQmGRohKglIMgTUGhhA 21, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 21, 30 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 17 Feb 2009 10:58:51 -0600 21, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 21, 30 -- Tue, 17 Feb 2009 10:58:51 -0600 21, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 30 -- Content-class: urn:content-classes:message 21, 30 -- MIME-Version: 1.0 21, 30 -- Content-Type: text/plain; 21, 30 -- charset="us-ascii" 21, 30 -- Subject: RE: [Microscopy] A challenge 21, 30 -- Date: Tue, 17 Feb 2009 10:58:50 -0600 21, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7E11-at-UM-XMAIL08.um.umsystem.edu} 21, 30 -- In-Reply-To: {200902171637.n1HGb41P027702-at-ns.microscopy.com} 21, 30 -- X-MS-Has-Attach: 21, 30 -- X-MS-TNEF-Correlator: 21, 30 -- Thread-Topic: [Microscopy] A challenge 21, 30 -- Thread-Index: AcmRHfi8z7B9/rL5Tm+yTCNGPLmEKAAAqLfA 21, 30 -- References: {200902171637.n1HGb41P027702-at-ns.microscopy.com} 21, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 21, 30 -- To: {tpepper-at-iastate.edu} 21, 30 -- Cc: {microscopy-at-microscopy.com} 21, 30 -- X-OriginalArrivalTime: 17 Feb 2009 16:58:51.0223 (UTC) FILETIME=[021DDE70:01C99121] 21, 30 -- Content-Transfer-Encoding: 8bit 21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HGwqQ3002803 ==============================End of - Headers==============================
What sort of EM do you want to do? Your first question is about embedding for ultramicrotomy and transmission EM, your second question is about coating for scanning EM. If the latter, don't embed, just cryofracture the paper in liquid nitrogen, mount on edge (support the paper with silver-paint coated pieces of applicator stick or the like), or on a stub with a tilted surface, and sputter-coat with gold or better, gold-palladium. If you have a low-voltage or environmental/low-vacuum SEM, you may not need to coat. Are the fibers wood (or other plant) or polymer or ... ? This works for both coated and uncoated papers. Just be sure the samples are dry.
Phil
} Hi, all. } } We want to be able to closely examine the fibers } in cross-sections of various types and grades of } paper. } } The first question we need to answer is what } material to embed with, and which type of } microtome would be best to use to make the } cross-sections? The second question is how } important is sputter-coating and does anyone } have a recommendation regarding the material to } coat with? Lastly, to avoid re-inventing the } wheel, does anyone have images of this type that } they'd be willing to share? } } Robert Zonis } Sanford L.P.Ý- A Newell Rubbermaid Company } Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } robert.zonis-at-sanford.com -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 27 -- From oshel1pe-at-cmich.edu Tue Feb 17 11:14:42 2009 4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHEftn017299 4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:14:42 -0600 4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n1HHEaiF017173 4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:14:38 -0500 4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 27 -- Tue, 17 Feb 2009 12:14:09 -0500 4, 27 -- Mime-Version: 1.0 4, 27 -- Message-Id: {f06240800c5c09f3eda17-at-[141.209.160.249]} 4, 27 -- In-Reply-To: {200902171621.n1HGLA1D010929-at-ns.microscopy.com} 4, 27 -- References: {200902171621.n1HGLA1D010929-at-ns.microscopy.com} 4, 27 -- Date: Tue, 17 Feb 2009 12:14:06 -0500 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 27 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades 4, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 4, 27 -- X-OriginalArrivalTime: 17 Feb 2009 17:14:09.0156 (UTC) FILETIME=[253F5440:01C99123] 4, 27 -- X-Canit-CHI2: 0.00 4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5) 4, 27 -- X-CanItPRO-Stream: default 4, 27 -- X-Canit-Stats-ID: 9043614 - f63b146406df 4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 4, 27 -- Content-Transfer-Encoding: 8bit 4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHEftn017299 ==============================End of - Headers==============================
I am a biologist too. I did help a user from Chemical Engineering to section a polymer. I simply throw the polymer (pre-stained with Osmium) into the Spurr resin, let it be "infiltrated" for a couple of hours, and put it in the oven to polymerize. The sections turned pretty well.
Give it a try, it might work.
Zhaojie Zhang Director, Microscopy Core Facility University of Wyoming Laramie, WY 82071 zzhng-at-uwyo.edu
-----Original Message----- X-from: tpepper-at-iastate.edu [mailto:tpepper-at-iastate.edu] Sent: Tuesday, February 17, 2009 9:41 AM To: Z.J. Zhang
Hello, I love being a biological service facility! I have a student from electrical engineering that has 'polymer' fibers that are water soluble and sensitive to temperatures below freezing. He wants TEM cross-sections of the fibers. 0_o
The gauntlet has been thrown, any ideas? Cryo's out due to temp issue, so can one section dry at room temp (provided the fibers don't dissolve in any resin)? Sorry I don't know the constitution of the fibers, just the few physical
issues with them-they apper to be thinner than, but similar in appearence to, spiderweb... (I love challenges!)
Thanks in advance!
Tracey Pepper Microscopy and NanoImaging Facility Iowa State University Ames, IA 50011-1020 (515) 294-3872
==============================Original Headers============================== 6, 21 -- From tpepper-at-iastate.edu Tue Feb 17 10:35:21 2009 6, 21 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HGZKEj023698 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:20 -0600 6, 21 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 6, 21 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n1HGZI0S017865 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:18 -0600 6, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 6, 21 -- id 5bfd_76ba80d0_fd0f_11dd_b413_001372578af6; 6, 21 -- Tue, 17 Feb 2009 10:24:34 -0600 6, 21 -- Received: from Debug (webmail-12.iastate.edu [129.186.140.32]) 6, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id n1HGZGrc024171 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 10:35:16 -0600 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu} 6, 21 -- Subject: A challenge 6, 21 -- Date: Tue, 17 Feb 2009 10:35:18 -0600 (CST) 6, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU Version mp9.11 6, 21 -- Message-Id: {1835101711092470-at-webmail.iastate.edu} 6, 21 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2009.2.17.162227 6, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_700_799 0, FROM_EDU_TLD 0, __C230066_P5 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __SANE_MSGID 0' ==============================End of - Headers==============================
==============================Original Headers============================== 18, 32 -- From ZZhang-at-uwyo.edu Tue Feb 17 11:27:10 2009 18, 32 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHR9JQ031609 18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:27:10 -0600 18, 32 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu [10.84.60.208]) 18, 32 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id n1HHOOXN017103; 18, 32 -- Tue, 17 Feb 2009 10:27:03 -0700 (MST) 18, 32 -- (envelope-from ZZhang-at-uwyo.edu) 18, 32 -- Received: from TELEGRAPH1.uwyo.edu (10.84.60.121) by ponyexpress-ht1.uwyo.edu 18, 32 -- (10.84.60.208) with Microsoft SMTP Server id 8.1.340.0; Tue, 17 Feb 2009 18, 32 -- 10:26:50 -0700 18, 32 -- Received: from TELEGRAPH5.uwyo.edu ([10.84.60.120]) by TELEGRAPH1.uwyo.edu 18, 32 -- with Microsoft SMTPSVC(6.0.3790.3959); Tue, 17 Feb 2009 10:26:49 -0700 18, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 32 -- Content-Class: urn:content-classes:message 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Type: text/plain; charset="US-ASCII" 18, 32 -- Subject: RE: [Microscopy] A challenge 18, 32 -- Date: Tue, 17 Feb 2009 10:26:48 -0700 18, 32 -- Message-ID: {D0162B092A0F46429960E910DE8B3C4902F5476D-at-TELEGRAPH5.uwyo.edu} 18, 32 -- In-Reply-To: {200902171641.n1HGf14x006121-at-ns.microscopy.com} 18, 32 -- X-MS-Has-Attach: 18, 32 -- X-MS-TNEF-Correlator: 18, 32 -- Thread-Topic: [Microscopy] A challenge 18, 32 -- Thread-Index: AcmRHolWhQE/Vu6/SgSXWBf4/AN3DQABKkhw 18, 32 -- References: {200902171641.n1HGf14x006121-at-ns.microscopy.com} 18, 32 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 18, 32 -- To: {microscopy-at-microscopy.com} 18, 32 -- CC: {tpepper-at-iastate.edu} 18, 32 -- X-OriginalArrivalTime: 17 Feb 2009 17:26:49.0595 (UTC) FILETIME=[EA811CB0:01C99124] 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHR9JQ031609 ==============================End of - Headers==============================
You want to view a cross section of the papers, but do not necessarily need to create a thin section? If so, mount the papers in epoxy and polish to achieve a cross section. You may not need to coat if you have an SEM capable of low voltage and/or low vacuum.
What is your end objective - that would help in determining the best sample prep.
I have examined cross sections of paper before using embedding and polishing, however it might not be suitable for your purposes.
We can discuss more off-line if you wish and share images.
Jacqueline
Jacqueline Ayotte Microscopist - Advanced Materials Characterization Ticona 8040 Dixie Highway } Florence KY 41042 } 859-372-3139 } fax 859-372-3184 } jacqueline.ayotte-at-ticona.com The information contained in this e-mail, and any attachments thereto, is confidential and is intended only for use by the individual(s) and/or entity named above. If you are not the intended recipient of this e-mail, you are hereby notified that any dissemination, distribution or copying of this communication or any disclosure of the contents of this communication to others is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this e-mail. Please then delete the original including all attachments and any copy of any e-mail and printout thereof.
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What sort of EM do you want to do? Your first question is about embedding for ultramicrotomy and transmission EM, your second question is about coating for scanning EM. If the latter, don't embed, just cryofracture the paper in liquid nitrogen, mount on edge (support the paper with silver-paint coated pieces of applicator stick or the like), or on a stub with a tilted surface, and sputter-coat with gold or better, gold-palladium. If you have a low-voltage or environmental/low-vacuum SEM, you may not need to coat. Are the fibers wood (or other plant) or polymer or ... ? This works for both coated and uncoated papers. Just be sure the samples are dry.
Phil
} Hi, all. } } We want to be able to closely examine the fibers in cross-sections of } various types and grades of paper. } } The first question we need to answer is what material to embed with, } and which type of microtome would be best to use to make the } cross-sections? The second question is how important is sputter-coating } and does anyone have a recommendation regarding the material to coat } with? Lastly, to avoid re-inventing the wheel, does anyone have images } of this type that they'd be willing to share? } } Robert Zonis } Sanford L.P.Ý- A Newell Rubbermaid Company Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } robert.zonis-at-sanford.com -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 27 -- From oshel1pe-at-cmich.edu Tue Feb 17 11:14:42 2009 4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHEftn017299 4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:14:42 -0600 4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n1HHEaiF017173 4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:14:38 -0500 4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 27 -- Tue, 17 Feb 2009 12:14:09 -0500 4, 27 -- Mime-Version: 1.0 4, 27 -- Message-Id: {f06240800c5c09f3eda17-at-[141.209.160.249]} 4, 27 -- In-Reply-To: {200902171621.n1HGLA1D010929-at-ns.microscopy.com} 4, 27 -- References: {200902171621.n1HGLA1D010929-at-ns.microscopy.com} 4, 27 -- Date: Tue, 17 Feb 2009 12:14:06 -0500 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 27 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades 4, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 4, 27 -- X-OriginalArrivalTime: 17 Feb 2009 17:14:09.0156 (UTC) FILETIME=[253F5440:01C99123] 4, 27 -- X-Canit-CHI2: 0.00 4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5) 4, 27 -- X-CanItPRO-Stream: default 4, 27 -- X-Canit-Stats-ID: 9043614 - f63b146406df 4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 4, 27 -- Content-Transfer-Encoding: 8bit 4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHEftn017299 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 31 -- From Jacqueline.Ayotte-at-ticona.com Tue Feb 17 11:45:34 2009 16, 31 -- Received: from exprod7og106.obsmtp.com (exprod7og106.obsmtp.com [64.18.2.165]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1HHjUsu013517 16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:45:32 -0600 16, 31 -- Received: from source ([148.163.79.14]) by exprod7ob106.postini.com ([64.18.6.12]) with SMTP 16, 31 -- ID DSNKSZr3sTgJhZC9gGIrSbv0Ov60aofupNoK-at-postini.com; Tue, 17 Feb 2009 09:45:33 PST 16, 31 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by smtp1.celanese.com with Microsoft SMTPSVC(6.0.3790.3959); 16, 31 -- Tue, 17 Feb 2009 11:52:48 -0600 16, 31 -- Received: from amalnxmbx1.SW.CZDS.BZ ([148.163.82.91]) by amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959); 16, 31 -- Tue, 17 Feb 2009 11:45:13 -0600 16, 31 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by amalnxmbx1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959); 16, 31 -- Tue, 17 Feb 2009 11:45:12 -0600 16, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 31 -- Content-class: urn:content-classes:message 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Type: text/plain; 16, 31 -- charset="iso-8859-1" 16, 31 -- Subject: RE: [Microscopy] Re: EM - Cross-sections of paper grades 16, 31 -- Date: Tue, 17 Feb 2009 12:45:10 -0500 16, 31 -- Message-ID: {A13B1B747CE9A748BE639EB5A1ACE126013D7229-at-amfloxmbx1.SW.CZDS.BZ} 16, 31 -- In-Reply-To: {200902171724.n1HHOWK5031290-at-ns.microscopy.com} 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- Thread-Topic: [Microscopy] Re: EM - Cross-sections of paper grades 16, 31 -- Thread-Index: AcmRJJxAYhx2j44lR7y2CBw5iJwSgAAAWU2w 16, 31 -- References: {200902171724.n1HHOWK5031290-at-ns.microscopy.com} 16, 31 -- From: "Ayotte, Jacqueline M., Ticona/US" {Jacqueline.Ayotte-at-ticona.com} 16, 31 -- To: {Microscopy-at-microscopy.com} 16, 31 -- X-OriginalArrivalTime: 17 Feb 2009 17:45:12.0928 (UTC) FILETIME=[7C245E00:01C99127] 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHjUsu013517 ==============================End of - Headers==============================
Ah! Great idea, thanks! I had problems embedding dollar bills.
I love this list., no bickering no fighting, just good info.
Tom Kaye
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Tuesday, February 17, 2009 10:20 AM To: tom-at-tomkaye.com
What sort of EM do you want to do? Your first question is about embedding for ultramicrotomy and transmission EM, your second question is about coating for scanning EM. If the latter, don't embed, just cryofracture the paper in liquid nitrogen, mount on edge (support the paper with silver-paint coated pieces of applicator stick or the like), or on a stub with a tilted surface, and sputter-coat with gold or better, gold-palladium. If you have a low-voltage or environmental/low-vacuum SEM, you may not need to coat. Are the fibers wood (or other plant) or polymer or ... ? This works for both coated and uncoated papers. Just be sure the samples are dry.
Phil
} Hi, all. } } We want to be able to closely examine the fibers } in cross-sections of various types and grades of } paper. } } The first question we need to answer is what } material to embed with, and which type of } microtome would be best to use to make the } cross-sections? The second question is how } important is sputter-coating and does anyone } have a recommendation regarding the material to } coat with? Lastly, to avoid re-inventing the } wheel, does anyone have images of this type that } they'd be willing to share? } } Robert Zonis } Sanford L.P.Ý- A Newell Rubbermaid Company } Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } robert.zonis-at-sanford.com -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 27 -- From oshel1pe-at-cmich.edu Tue Feb 17 11:14:42 2009 4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHEftn017299 4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:14:42 -0600 4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n1HHEaiF017173 4, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:14:38 -0500 4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 27 -- Tue, 17 Feb 2009 12:14:09 -0500 4, 27 -- Mime-Version: 1.0 4, 27 -- Message-Id: {f06240800c5c09f3eda17-at-[141.209.160.249]} 4, 27 -- In-Reply-To: {200902171621.n1HGLA1D010929-at-ns.microscopy.com} 4, 27 -- References: {200902171621.n1HGLA1D010929-at-ns.microscopy.com} 4, 27 -- Date: Tue, 17 Feb 2009 12:14:06 -0500 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 27 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades 4, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 4, 27 -- X-OriginalArrivalTime: 17 Feb 2009 17:14:09.0156 (UTC) FILETIME=[253F5440:01C99123] 4, 27 -- X-Canit-CHI2: 0.00 4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5) 4, 27 -- X-CanItPRO-Stream: default 4, 27 -- X-Canit-Stats-ID: 9043614 - f63b146406df 4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 4, 27 -- Content-Transfer-Encoding: 8bit 4, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HHEftn017299 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 22 -- From tom-at-TomKaye.com Tue Feb 17 11:52:01 2009 15, 22 -- Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com [66.102.127.13]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HHq0Jo021955 15, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 11:52:00 -0600 15, 22 -- Received: from TKdual ([67.134.109.68]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959); 15, 22 -- Tue, 17 Feb 2009 11:51:58 -0600 15, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com} 15, 22 -- To: {microscopy-at-microscopy.com} 15, 22 -- Subject: RE: [Microscopy] Re: EM - Cross-sections of paper grades 15, 22 -- Date: Tue, 17 Feb 2009 10:53:53 -0700 15, 22 -- Message-ID: {GOEAIJOJDGCJBPIFHOBGOEFIADAB.tom-at-tomkaye.com} 15, 22 -- MIME-Version: 1.0 15, 22 -- Content-Type: text/plain; 15, 22 -- charset="iso-8859-1" 15, 22 -- Content-Transfer-Encoding: 8bit 15, 22 -- X-Priority: 3 (Normal) 15, 22 -- X-MSMail-Priority: Normal 15, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 15, 22 -- Importance: Normal 15, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 15, 22 -- In-Reply-To: {200902171720.n1HHKEKo023615-at-ns.microscopy.com} 15, 22 -- X-OriginalArrivalTime: 17 Feb 2009 17:51:58.0364 (UTC) FILETIME=[6DCCFDC0:01C99128] ==============================End of - Headers==============================
That helps a lot, Phil. I couldn't figure out how to get a clean cross-section without a microtome, so I asked about TEM techniques. As a novice at TEM and sputter coating, I was hoping that I'd be able to run both scanning and transmission EM with samples from the same block of embedded paper sample - it sounds like that's not possible.
We do have a low-vacuum SEM on-site here, but I just assumed I'd have to do most or all of this study with a STEM at our local university. If that liquid nitrogen technique gives me good edges, I'll be able to do this here.
We want to look at standard, ordinary wood-fiber paper, with and without various types of ink written on it, hopefully being able to see how the inks interact with and penetrate into the paper fibers and sizing agents.
Robert Zonis
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Tuesday, February 17, 2009 11:22 AM To: Zonis, Robert
Robert,
I remember JEOL recently sending me a newsletter on this subject. If you go to their web site, www.jeolusa.com, you can get information on cross-sectioning paper for SEM examination. They also have images of paper cross sections you can look at. On their site, navigate by clicking on Products/Sample Preparation Equipment/Cross Section Polisher. On that page you can click on their Image Gallery. Naturally, this is all centered around the Cross Section Polisher that they are marketing.
Disclaimer: I have no financial interests in JEOL. I'm only a satisfied user of their products.
Stu Smalinskas, P.E. Metallurgist SKF USA Plymouth, Michigan
--- On Tue, 2/17/09, Robert.Zonis-at-Sanford.com {Robert.Zonis-at-Sanford.com} wrote: } } Hi, all. } } We want to be able to closely examine the fibers in } cross-sections of various types and grades of paper. } } The first question we need to answer is what material to } embed with, and which type of microtome would be best to use } to make the cross-sections? The second question is how } important is sputter-coating and does anyone have a } recommendation regarding the material to coat with? Lastly, } to avoid re-inventing the wheel, does anyone have images of } this type that they'd be willing to share? } } Robert Zonis } Sanford L.P. - A Newell Rubbermaid Company } Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } robert.zonis-at-sanford.com }
==============================Original Headers============================== 9, 23 -- From smalinskas-at-yahoo.com Tue Feb 17 13:00:00 2009 9, 23 -- Received: from web34404.mail.mud.yahoo.com (web34404.mail.mud.yahoo.com [66.163.178.153]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1HIxvWL025101 9, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Tue, 17 Feb 2009 12:59:59 -0600 9, 23 -- Received: (qmail 62770 invoked by uid 60001); 17 Feb 2009 18:59:55 -0000 9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 23 -- s=s1024; d=yahoo.com; 9, 23 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 23 -- b=163EFy8gZwjqHzNn6SvhZJ7w87kcHF21wd6FtLBYOP/BA1r8dhA0C7+CCCnpTLMnuuO9kcOcXjXaKFXsmAEMl2jw/lgR0cjEBII1jF/fpgu2WvoBK0EYDnZgmaA9nmFoAcDhDqpQZ/n3h147ayGZgESLeCTi38520e2DN5iUYrs=; 9, 23 -- X-YMail-OSG: bCGw8VwVM1klOXHiR5GrghrkcQixLyzkzjmtMeiEYIBFVhAruL8zejea8C5iIgh7q9SxlVc91XcwzNsMGBeMsq_GXe_5Vspc5RNFNQN5r6dDMD5.78j1wz1Ujzwfcur19RopWG0dI.0gQOutoLpfB4UPl1tlBpFdUdZZ7F8dN0ed5lIO.vGPjM1wStElFzrds6xc7QjDjMeA_lDyXjOWH1e_jqldngQ- 9, 23 -- Received: from [141.151.33.213] by web34404.mail.mud.yahoo.com via HTTP; Tue, 17 Feb 2009 10:59:55 PST 9, 23 -- X-Mailer: YahooMailWebService/0.7.260.1 9, 23 -- Date: Tue, 17 Feb 2009 10:59:55 -0800 (PST) 9, 23 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 9, 23 -- Reply-To: smalinskas-at-yahoo.com 9, 23 -- Subject: Re: [Microscopy] EM - Cross-sections of paper grades 9, 23 -- To: Robert.Zonis-at-Sanford.com, microscopy-at-ns.microscopy.com 9, 23 -- In-Reply-To: {200902171618.n1HGIibW006216-at-ns.microscopy.com} 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=iso-8859-1 9, 23 -- Message-ID: {305999.62206.qm-at-web34404.mail.mud.yahoo.com} 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1HIxvWL025101 ==============================End of - Headers==============================
This posting is from one of my users. Please reply directly to Dr. Hailiang Dong's contact information below.
====
Geology: Postdoctoral Fellow (position is pending funding) to perform mineralogical characterizations using X-ray diffraction, scanning and transmission electron microscopy (SEM and TEM), and mineral-microbe interactions; install and set up new TEM; work in a team in the field including site characterization and core collection as well as numerical modeling.
Require: Ph.D in mineralogy or materials science; extensive experience in TEM, electron energy loss spectroscopy (EELS), mineral-microbe interactions (microbial reduction and oxidation of metals), and wet chemistry; good oral and written communication skills.
Desire: Experience in synchrotron-based techniques, Mossbauer spectroscopy, molecular microbiology, microbial ecology, and/or metagenomics and microarrays. This is a one-year appointment with re-appointment subject to continued funding and satisfactory performance.
Send letter of application, curriculum vitae, and names and contact information for three references to:
Dr. Hailiang Dong Department of Geology Miami University Oxford, OH 45056.
Contact phone number is 513-529-2517 Email is dongh-at-muohio.edu.
Review of applications will begin on April 1, 2009 and will continue until the position is filled.
Miami University is an affirmative action/equal opportunity employer with smoke-free campuses. For information regarding campus crime and safety, visit www.muohio.edu/righttoknow. Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 12, 22 -- From edelmare-at-muohio.edu Tue Feb 17 13:29:05 2009 12, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1HJT4rB007605 12, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Feb 2009 13:29:04 -0600 12, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 12, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n1HJT6fE019007 12, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Feb 2009 14:29:06 -0500 12, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 12, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n1HJT3NO022524 12, 22 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Feb 2009 14:29:03 -0500 12, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 22 -- To: microscopy-at-Microscopy.com 12, 22 -- Date: Tue, 17 Feb 2009 14:29:03 -0500 12, 22 -- MIME-Version: 1.0 12, 22 -- Subject: Post-Doc Position 12, 22 -- Message-ID: {499AC9AF.17804.68FBC15-at-edelmare.muohio.edu} 12, 22 -- Priority: normal 12, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 22 -- Content-type: text/plain; charset=US-ASCII 12, 22 -- Content-transfer-encoding: 7BIT 12, 22 -- Content-description: Mail message body 12, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70 ==============================End of - Headers==============================
A group across the road at the ANU were looking at ink penetration into paper, I read about it in one of their monthly research blurbs. I guess the list doesn't accept attachments, but you can check out their stuff at http://www.anu.edu.au/CSEM/newsletters/2003/Nov03.pdf and there's a bit more here: http://www.anu.edu.au/CSEM/newsletters/2005/MMMar05.pdf
The upshot was essentially as suggested by Phil - snap-freeze at different stages of ink penetration and observe, still frozen, by SEM - the snag is that it does require a cryostage in the microscope, though if you have the sample attached to a big enough lump of metal that's very cold, the sample will stay cold for quite a while.
Aha, I found a reference: Roberts R, Senden T, Knackstedt M, Lyne MB (2003) Spreading of aqueous liquids in unsized papers is by film flow. Journal of Pulp and Paper Science 29: 123-131
May be a bit off track, but I remember they had a lot of fun with this...
cheers, Roseamry
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 18/02/09 5:13 AM, "Robert.Zonis-at-Sanford.com" {Robert.Zonis-at-Sanford.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } That helps a lot, Phil. I couldn't figure out how to get a clean } cross-section without a microtome, so I asked about TEM techniques. As a } novice at TEM and sputter coating, I was hoping that I'd be able to run } both scanning and transmission EM with samples from the same block of } embedded paper sample - it sounds like that's not possible. } } We do have a low-vacuum SEM on-site here, but I just assumed I'd have to } do most or all of this study with a STEM at our local university. If } that liquid nitrogen technique gives me good edges, I'll be able to do } this here. } } We want to look at standard, ordinary wood-fiber paper, with and without } various types of ink written on it, hopefully being able to see how the } inks interact with and penetrate into the paper fibers and sizing } agents. } } Robert Zonis } } This message is intended for the Microscopy Listserv. Permission is } specifically granted to the Microscopy Society of America to publish } some or all of this message in the Microscopy Today journal. } } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: Tuesday, February 17, 2009 11:22 AM } To: Zonis, Robert } Subject: [Microscopy] Re: EM - Cross-sections of paper grades } } } What sort of EM do you want to do? Your first } question is about embedding for ultramicrotomy } and transmission EM, your second question is } about coating for scanning EM. } If the latter, don't embed, just cryofracture the } paper in liquid nitrogen, mount on edge (support } the paper with silver-paint coated pieces of } applicator stick or the like), or on a stub with } a tilted surface, and sputter-coat with gold or } better, gold-palladium. If you have a low-voltage } or environmental/low-vacuum SEM, you may not need } to coat. Are the fibers wood (or other plant) or } polymer or ... ? } This works for both coated and uncoated papers. } Just be sure the samples are dry. } } Phil } } } This message may contain information that is confidential and/or protected by } law. If the reader of this message is not the intended recipient, you are } hereby notified that any dissemination, distribution, copying or communication } of this message is strictly prohibited. If you have received this } communication in error, please contact the sender immediately and delete the } message. Please note that although we will take all commercially reasonable } efforts to prevent viruses from being transmitted from our systems, it is the } responsibility of the recipient to check for and prevent adverse action by } viruses on its own systems. } } ______________________________________________________________________ } This email has been scanned by the MessageLabs Email Security System. } For more information please visit http://www.messagelabs.com/email } ______________________________________________________________________ } } } ==============================Original Headers============================== } 14, 34 -- From Robert.Zonis-at-Sanford.com Tue Feb 17 12:06:25 2009 } 14, 34 -- Received: from mail96.messagelabs.com (mail96.messagelabs.com } [216.82.254.19]) } 14, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n1HI6NIO009186 } 14, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Feb 2009 12:06:24 -0600 } 14, 34 -- X-VirusChecked: Checked } 14, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com } 14, 34 -- X-Msg-Ref: server-2.tower-96.messagelabs.com!1234893978!55739307!3 } 14, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- } 14, 34 -- X-Originating-IP: [198.176.16.26] } 14, 34 -- Received: (qmail 19039 invoked from network); 17 Feb 2009 18:06:20 } -0000 } 14, 34 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) } (198.176.16.26) } 14, 34 -- by server-2.tower-96.messagelabs.com with RC4-SHA encrypted SMTP; } 17 Feb 2009 18:06:20 -0000 } 14, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by } naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); } 14, 34 -- Tue, 17 Feb 2009 12:06:19 -0600 } 14, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 14, 34 -- Content-class: urn:content-classes:message } 14, 34 -- MIME-Version: 1.0 } 14, 34 -- Content-Type: text/plain; } 14, 34 -- charset="US-ASCII" } 14, 34 -- Subject: RE: [Microscopy] Re: EM - Cross-sections of paper grades } 14, 34 -- Date: Tue, 17 Feb 2009 12:03:14 -0600 } 14, 34 -- Message-ID: } {66260BA266051B4FA0EC9EA3B33E6A9401B6CA4A-at-naoaksasebe02.nr.ad.newellco.com} } 14, 34 -- In-Reply-To: {200902171722.n1HHME0D026703-at-ns.microscopy.com} } 14, 34 -- X-MS-Has-Attach: } 14, 34 -- X-MS-TNEF-Correlator: } 14, 34 -- Thread-Topic: [Microscopy] Re: EM - Cross-sections of paper grades } 14, 34 -- Thread-Index: AcmRJFwPAdBdcY/AR2SFHvrxwphFVQAAm65Q } 14, 34 -- References: {200902171722.n1HHME0D026703-at-ns.microscopy.com} } 14, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} } 14, 34 -- To: {oshel1pe-at-cmich.edu} } 14, 34 -- Cc: {Microscopy-at-microscopy.com} } 14, 34 -- X-OriginalArrivalTime: 17 Feb 2009 18:06:19.0704 (UTC) } FILETIME=[6F330B80:01C9912A] } 14, 34 -- Content-Transfer-Encoding: 8bit } 14, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n1HI6NIO009186 } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jacqueline.ayotte-at-ticona.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: I am interested in being able to microtome glass filled polymers. I currently microtome using glass knives, which works well for unfilled polymeric materials but not glass filled. My end goal is to view crystalline morphology of glass filled polymers as I currently do with unfilled polymers.
Does anyone currently do this and what equipment are you using?
We have a SEM Topcon SM510 that needs some help: our monitor board burned. I am wondering if anyone else suffered such a disaster and can suggest us where we can repair the CRT or buy another equivalent monitor ? Feel free to contact me off list.
Thanks for your help Best Regards
Daniela Gambaro
==============================Original Headers============================== 7, 24 -- From DGambaro-at-memc.it Wed Feb 18 05:08:22 2009 7, 24 -- Received: from mail.memc.it (host18-139-static.43-88-b.business.telecomitalia.it [88.43.139.18]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IB8Lj1009599 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 05:08:22 -0600 7, 24 -- Received: from novlnml1.eur.memc.com ([167.170.160.16]) 7, 24 -- by mail.memc.it (Lotus Domino Release 6.5.6FP3) 7, 24 -- with ESMTP id 2009021812082100-11084 ; 7, 24 -- Wed, 18 Feb 2009 12:08:21 +0100 7, 24 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 7, 24 -- MIME-Version: 1.0 7, 24 -- Subject: SEM - Need help on Topcon sm510 repair 7, 24 -- To: {Microscopy-at-microscopy.com} 7, 24 -- From: DGambaro-at-memc.it 7, 24 -- Message-ID: {OFA7E4DE46.6EBED0FE-ONC1257561.003989C6-C1257561.003D30B2-at-memc.com} 7, 24 -- Date: Wed, 18 Feb 2009 12:08:21 +0100 7, 24 -- X-MIMETrack: Serialize by Router on NOVLNML1/NOV/MEMC(Release 6.5.6FP3|March 27, 2008) at 7, 24 -- 18/02/2009 12.08.20, 7, 24 -- Serialize complete at 18/02/2009 12.08.20, 7, 24 -- Itemize by SMTP Server on NOVLNHB1/NOV/MEMC(Release 6.5.6FP3|March 27, 2008) at 7, 24 -- 18/02/2009 12.08.21, 7, 24 -- Serialize by Router on NOVLNHB1/NOV/MEMC(Release 6.5.6FP3|March 27, 2008) at 7, 24 -- 18/02/2009 12.08.22, 7, 24 -- Serialize complete at 18/02/2009 12.08.22 7, 24 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Trying to locate a lab in the North Texas area that has Laser 3D Surface profile capabilities. Need to profile surface of a laser treated polyimide film on glass substrate to nanometer resolution. SEM images have been very difficult to achieve due to charging and small feature size. Thinking a surface profiler or AFM may give better results.
Prefer University labs but will be glad to hear from commercial labs as well.
Thanks
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 26 -- From rbeavers-at-mail.smu.edu Wed Feb 18 12:43:46 2009 7, 26 -- Received: from sxet1p1.systems.smu.edu (sxet1p1.systems.smu.edu [129.119.65.146]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IIhhhn011183 7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:43:46 -0600 7, 26 -- Received: from sxht1p1.systems.smu.edu (129.119.65.132) by 7, 26 -- sxet1p1.systems.smu.edu (129.119.65.146) with Microsoft SMTP Server (TLS) id 7, 26 -- 8.1.340.0; Wed, 18 Feb 2009 12:43:50 -0600 7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by 7, 26 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Wed, 18 Feb 2009 7, 26 -- 12:43:40 -0600 7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 26 -- Date: Wed, 18 Feb 2009 12:43:39 -0600 7, 26 -- Subject: Laser Surface Profiler 7, 26 -- Thread-Topic: Laser Surface Profiler 7, 26 -- Thread-Index: AcmR+NCtXIRxFtvWRPKhtObGHr6YEg== 7, 26 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB053347F142-at-SXMBXC.systems.smu.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="iso-8859-1" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1IIhhhn011183 ==============================End of - Headers==============================
Couple of year ago I used services from Optipro www.optipro.com, they sell laser profiling equipment and did provide lab services for a reasonable fee; not in TX though, NY.
I worked with Lynda Bechtold lynda-at-optipro.com
No commercial interest, just a satisfied customer :)
Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Wednesday, February 18, 2009 1:48 PM To: vray-at-partbeamsystech.com
Group,
Trying to locate a lab in the North Texas area that has Laser 3D Surface profile capabilities. Need to profile surface of a laser treated polyimide film on glass substrate to nanometer resolution. SEM images have been very difficult to achieve due to charging and small feature size. Thinking a surface profiler or AFM may give better results.
Prefer University labs but will be glad to hear from commercial labs as well.
Thanks
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 26 -- From rbeavers-at-mail.smu.edu Wed Feb 18 12:43:46 2009 7, 26 -- Received: from sxet1p1.systems.smu.edu (sxet1p1.systems.smu.edu [129.119.65.146]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IIhhhn011183 7, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:43:46 -0600 7, 26 -- Received: from sxht1p1.systems.smu.edu (129.119.65.132) by 7, 26 -- sxet1p1.systems.smu.edu (129.119.65.146) with Microsoft SMTP Server (TLS) id 7, 26 -- 8.1.340.0; Wed, 18 Feb 2009 12:43:50 -0600 7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by 7, 26 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Wed, 18 Feb 2009 7, 26 -- 12:43:40 -0600 7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 26 -- Date: Wed, 18 Feb 2009 12:43:39 -0600 7, 26 -- Subject: Laser Surface Profiler 7, 26 -- Thread-Topic: Laser Surface Profiler 7, 26 -- Thread-Index: AcmR+NCtXIRxFtvWRPKhtObGHr6YEg== 7, 26 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB053347F142-at-SXMBXC.systems.smu.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="iso-8859-1" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1IIhhhn011183 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From vray-at-partbeamsystech.com Wed Feb 18 13:22:08 2009 20, 26 -- Received: from smtp108.biz.mail.re2.yahoo.com (smtp108.biz.mail.re2.yahoo.com [206.190.52.47]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1IJM63B026685 20, 26 -- for {microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 13:22:07 -0600 20, 26 -- Message-Id: {200902181922.n1IJM63B026685-at-ns.microscopy.com} 20, 26 -- Received: (qmail 3741 invoked from network); 18 Feb 2009 19:22:04 -0000 20, 26 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.106.16 with login) 20, 26 -- by smtp108.biz.mail.re2.yahoo.com with SMTP; 18 Feb 2009 19:22:04 -0000 20, 26 -- X-YMail-OSG: LFPYeiEVM1kPdrQGo7aG.yFJOOwBa_IXD7U8E5vVfihPd8u3yCSQy0KM_2b8MN0Q8vjRGBrDQ_MD9Ic71UjiEYoF4v0SufDHBXYRpRexrEXpBV3iQ5l2vgk0R5oWFmpybVf18O2mdZ62R26eBQvpa5xBiO7._tvuxFoXO03sCEg4YPyrI1nkr24Yu5T9VXCwu2isao2fLJw7FK95uPG7TZf4 20, 26 -- X-Yahoo-Newman-Property: ymail-3 20, 26 -- Reply-To: {vray-at-partbeamsystech.com} 20, 26 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 20, 26 -- To: {rbeavers-at-mail.smu.edu} 20, 26 -- Cc: {microscopy-at-microscopy.com} 20, 26 -- Subject: RE: [Microscopy] Laser Surface Profiler 20, 26 -- Date: Wed, 18 Feb 2009 14:24:57 -0500 20, 26 -- Organization: PBST / MEO Engineering 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="iso-8859-1" 20, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 20, 26 -- Thread-Index: AcmR+V0Ama8AvtQtTeCfdtTcSAmpkgABCnTg 20, 26 -- In-Reply-To: {200902181847.n1IIlYnL012819-at-ns.microscopy.com} 20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1IJM63B026685 ==============================End of - Headers==============================
Hello I am in search for 4 inch wafers for a e-beam lithography research project, I have been searching for the past six months with no luck.
Dopant Orient. Resistance (ohm-cm) Phosphorus 1-0-0 0.1 Boron 1-0-0 0.1 Phosphorus 1-1-1 0.1 Boron 1-1-1 0.1 Side -Silicon w/ no film/coating (natural oxide up to 50nm ok) Other side - galvanic contact pad to base w/ AL coating
Wafer thickness cannot exceed 1mm Wafer thickness cannot be less than 0.3mm
Also am seeking for micro silica of the above wafers, if available. This is an ASAP matter, please contact me soon.
Could anyone provide possible sources to locate odd and specialty wafers?
Best Regards,
Anatoli Oleynik Specialty Consultant ProBiz Consulting
==============================Original Headers============================== 7, 37 -- From cell.toli-at-gmail.com Wed Feb 18 14:01:05 2009 7, 37 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.150]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IK14s0009391 7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 14:01:04 -0600 7, 37 -- Received: by qw-out-1920.google.com with SMTP id 14so20699qwa.54 7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:01:03 -0800 (PST) 7, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 37 -- d=gmail.com; s=gamma; 7, 37 -- h=domainkey-signature:received:received:message-id:date:from 7, 37 -- :user-agent:mime-version:to:subject:content-type 7, 37 -- :content-transfer-encoding; 7, 37 -- bh=d4aFgc5r/lyem2WGLj/OerFnq7wDSc9PYNA2RgU5+QI=; 7, 37 -- b=jBRIpDZLX70J5HI5A94DutjpwyO9sgTv+agfgj/vP5C+KjDfo44g7WrnXu927m9JBD 7, 37 -- rv3qiX5d5bTO+FkSvYS0Ezj10xAyYDxuOZdmT0FbsURBasLe0hgGVlFJqoPBQ9J+mk3p 7, 37 -- 5BNU5DEukMBK5bAHyJWV4nKzBz50K8dAtdhFI= 7, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 37 -- d=gmail.com; s=gamma; 7, 37 -- h=message-id:date:from:user-agent:mime-version:to:subject 7, 37 -- :content-type:content-transfer-encoding; 7, 37 -- b=TIoNrfjN/UVBg/eeDcyZ/zMRUg9eUY+pn5wRqGtHlMgD9xsqZ1nZ5CcUDLqTJ2dAvr 7, 37 -- ZXm0LBtGr7oZqX/0LolkgYtG0Asq9zEWlcxVhr0suNvjLYEGqPX2Tysed3iUM5A7TtwJ 7, 37 -- WCxfGvFYKDfjPFNemWI+E+F6pqRWUhwyLOUJQ= 7, 37 -- Received: by 10.229.85.17 with SMTP id m17mr3260973qcl.43.1234987259004; 7, 37 -- Wed, 18 Feb 2009 12:00:59 -0800 (PST) 7, 37 -- Received: from ?127.0.0.1? (cpe-065-190-187-114.nc.res.rr.com [65.190.187.114]) 7, 37 -- by mx.google.com with ESMTPS id 8sm417133ywg.54.2009.02.18.12.00.58 7, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 7, 37 -- Wed, 18 Feb 2009 12:00:58 -0800 (PST) 7, 37 -- Message-ID: {499C68FC.9020500-at-gmail.com} 7, 37 -- Date: Wed, 18 Feb 2009 15:01:00 -0500 7, 37 -- From: Anatoli Oleynik {cell.toli-at-gmail.com} 7, 37 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 7, 37 -- MIME-Version: 1.0 7, 37 -- To: Microscopy-at-microscopy.com 7, 37 -- Subject: Rare Wafers 7, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both susan.trant-at-viha.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: susan.trant-at-viha.ca Name: Susan Trant
Organization: Vancouver Island Health Authority
Title-Subject: [Filtered] Knife Makers
Question: I have an older LKB Bromma 7800 knife maker. I am looking for a vendor for the scoring blades. Does anyone know where I can purchase some more?
Sue Trant EM Technologist Vancouver Island Health Authority
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both david.mitchell-at-emu.usyd.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: david.mitchell-at-emu.usyd.edu.au Name: David Mitchell
Organization: EM Unit, University of Sydney
Title-Subject: [Filtered] TEM Philips CM120 Holders
Question: We have a venerable Philips TEM: CM120 Biofilter. This has been used mainly for biological work, but we have some materials folk wanting to look at ferromagnetic materials. For this we need holders with screw inserts, to ensure the foils don't get dislodged by the field (we've already had one disappear in the microscope, from the standard spring loaded holder). Being such an old model, I was wondering if any users had compatible holders from decommissioned machines they might be interested in passing on? We also have an even older CM12, and similar holders for this might also help us out. I am happy to discuss shipping charges - please email me directly. Thanks in advance and regards.
Dave Mitchell TEM Manager, EMU, University of Sydney
Anatoli Oleynik asked about a source for unusual Silicon and silica wafers. Try contacting Chris Baker of University Wafers at: Visit http://www.wafersale.com or email chris-at-wafersale.com or call Call 1-800-216-8349 / Fax 888-832-0340
disclaimer: I have no relationship with this company, even as a customer. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: cell.toli-at-gmail.com To: donc-at-asmicro.com Sent: Wednesday, February 18, 2009 3:06 PM Subject: [a] [Microscopy] Rare Wafers
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
Hello I am in search for 4 inch wafers for a e-beam lithography research project, I have been searching for the past six months with no luck.
Dopant Orient. Resistance (ohm-cm) Phosphorus 1-0-0 0.1 Boron 1-0-0 0.1 Phosphorus 1-1-1 0.1 Boron 1-1-1 0.1 Side -Silicon w/ no film/coating (natural oxide up to 50nm ok) Other side - galvanic contact pad to base w/ AL coating
Wafer thickness cannot exceed 1mm Wafer thickness cannot be less than 0.3mm
Also am seeking for micro silica of the above wafers, if available. This is an ASAP matter, please contact me soon.
Could anyone provide possible sources to locate odd and specialty wafers?
Best Regards,
Anatoli Oleynik Specialty Consultant ProBiz Consulting
==============================Original Headers============================== 7, 37 -- From cell.toli-at-gmail.com Wed Feb 18 14:01:05 2009 7, 37 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.150]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1IK14s0009391 7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 14:01:04 -0600 7, 37 -- Received: by qw-out-1920.google.com with SMTP id 14so20699qwa.54 7, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Feb 2009 12:01:03 -0800 (PST) 7, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 37 -- d=gmail.com; s=gamma; 7, 37 -- h=domainkey-signature:received:received:message-id:date:from 7, 37 -- :user-agent:mime-version:to:subject:content-type 7, 37 -- :content-transfer-encoding; 7, 37 -- bh=d4aFgc5r/lyem2WGLj/OerFnq7wDSc9PYNA2RgU5+QI=; 7, 37 -- b=jBRIpDZLX70J5HI5A94DutjpwyO9sgTv+agfgj/vP5C+KjDfo44g7WrnXu927m9JBD 7, 37 -- rv3qiX5d5bTO+FkSvYS0Ezj10xAyYDxuOZdmT0FbsURBasLe0hgGVlFJqoPBQ9J+mk3p 7, 37 -- 5BNU5DEukMBK5bAHyJWV4nKzBz50K8dAtdhFI= 7, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 37 -- d=gmail.com; s=gamma; 7, 37 -- h=message-id:date:from:user-agent:mime-version:to:subject 7, 37 -- :content-type:content-transfer-encoding; 7, 37 -- b=TIoNrfjN/UVBg/eeDcyZ/zMRUg9eUY+pn5wRqGtHlMgD9xsqZ1nZ5CcUDLqTJ2dAvr 7, 37 -- ZXm0LBtGr7oZqX/0LolkgYtG0Asq9zEWlcxVhr0suNvjLYEGqPX2Tysed3iUM5A7TtwJ 7, 37 -- WCxfGvFYKDfjPFNemWI+E+F6pqRWUhwyLOUJQ= 7, 37 -- Received: by 10.229.85.17 with SMTP id m17mr3260973qcl.43.1234987259004; 7, 37 -- Wed, 18 Feb 2009 12:00:59 -0800 (PST) 7, 37 -- Received: from ?127.0.0.1? (cpe-065-190-187-114.nc.res.rr.com [65.190.187.114]) 7, 37 -- by mx.google.com with ESMTPS id 8sm417133ywg.54.2009.02.18.12.00.58 7, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 7, 37 -- Wed, 18 Feb 2009 12:00:58 -0800 (PST) 7, 37 -- Message-ID: {499C68FC.9020500-at-gmail.com} 7, 37 -- Date: Wed, 18 Feb 2009 15:01:00 -0500 7, 37 -- From: Anatoli Oleynik {cell.toli-at-gmail.com} 7, 37 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 7, 37 -- MIME-Version: 1.0 7, 37 -- To: Microscopy-at-microscopy.com 7, 37 -- Subject: Rare Wafers 7, 37 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 18, 25 -- From donc-at-asmicro.com Thu Feb 19 11:00:58 2009 18, 25 -- Received: from smtp109.sbc.mail.re2.yahoo.com (smtp109.sbc.mail.re2.yahoo.com [68.142.229.96]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1JH0vrf029985 18, 25 -- for {microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 11:00:57 -0600 18, 25 -- Received: (qmail 59861 invoked from network); 19 Feb 2009 17:00:57 -0000 18, 25 -- Received: from unknown (HELO asm15) (donc-at-76.240.194.228 with login) 18, 25 -- by smtp109.sbc.mail.re2.yahoo.com with SMTP; 19 Feb 2009 17:00:56 -0000 18, 25 -- X-YMail-OSG: usQbrN0VM1mjAkpGFX8JohEOdoXLuo6e2QrFqK4U4kJp3.ITYR1HcR8.CYOd20mGiSl3ZITmNKU2CHxK4CrkRdzqBz_.1p.Wx9r1aCQ47eKE_hvJcnQsMA5Os7dZbkxQDV_YDeij7m9_bGbiyZGxlcZGARldlVYoyhgea2GHcApRakdduRtZapmcXMjJRvWrLGNhtt_O3ak5RAgJqKMdyHwJcQzz 18, 25 -- X-Yahoo-Newman-Property: ymail-3 18, 25 -- Message-ID: {E12AC932284949C79C69D20CE5B324CD-at-asm15} 18, 25 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 18, 25 -- To: {cell.toli-at-gmail.com} , "Microscopy List" {microscopy-at-microscopy.com} 18, 25 -- References: {200902182006.n1IK6352014039-at-ns.microscopy.com} 18, 25 -- Subject: Re: [a] [Microscopy] Rare Wafers 18, 25 -- Date: Thu, 19 Feb 2009 12:00:40 -0500 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- format=flowed; 18, 25 -- charset="iso-8859-1"; 18, 25 -- reply-type=original 18, 25 -- Content-Transfer-Encoding: 7bit 18, 25 -- X-Priority: 3 18, 25 -- X-MSMail-Priority: Normal 18, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Director, CAMCOR Transmission Electron Microscopy Facility The Center for Advanced Materials Characterization in Oregon, the Materials Science Institute (MSI) at the University of Oregon (UO) in Eugene in partnership with the Oregon Nanoscience and Microtechnologies Institute (ONAMI) is currently seeking applications for a Director for CAMCOR's Transmission Electron Microscopy (TEM) Facility. The position of Director for the CAMCOR TEM Facility requires a high energy, responsible, efficient and meticulous manager/analyst that is focused on ensuring that the facility is reliably operational and available to all faculty, students and commercial users on a daily basis. The Director is required to manage the facility budget and ensure that a sound business model is implemented. A vision for making the facility a leader in the northwest and passion for expanding the impact of the facility among ONAMI researchers is desired. A PhD in chemistry, physics, materials science, or related field is required or five years experience as facilities director of a TEM facility. Expertise in electron diffraction, imaging techniques, analytical techniques, and high-resolution electron microscopy (HREM) is required. Experience working with aberration corrected TEM is preferred. The successful candidate will have the ability to work effectively with faculty, staff and students from a variety of diverse backgrounds. Applicants should submit a cover letter, curriculum vitae and arrange for three reference letters to be sent electronically to Carol Hanson at carolhan-at-uoregon.edu. Review will begin 3/20/09 and position will remain open until filled. For full position announcement, see
An equal-opportunity, affirmative-action institution committed to cultural diversity and compliance with the Americans with Disabilities Act.
POSITION DESCRIPTION Terms of Appointment Position title: Director, CAMCOR Transmission Electron Microscopy Facility Appointment percent: 0.8 fte Type of appointment: Fixed-term Annual base rate/range: $70,000-100,000 Annual Basis: 12 Starting date: April 2009
Essential Functions The Center for Advanced Materials Characterization in Oregon, the Materials Science Institute (MSI) at the University of Oregon (UO) in Eugene in partnership with the Oregon Nanoscience and Microtechnologies Institute (ONAMI) is currently seeking applications for a Director for CAMCOR's Transmission Electron Microscopy (TEM) Facility.
The position of Director for the CAMCOR TEM Facility requires a high energy, responsible, efficient and meticulous manager/analyst that is focused on ensuring that the facility is reliably operational and available to all faculty, students and commercial users on a daily basis. The Director is required to manage the facility budget and ensure that a sound business model is implemented. A vision for making the facility a leader in the northwest and passion for expanding the impact of the facility among ONAMI researchers is desired.
Minimum and Preferred Qualifications A PhD in chemistry, physics, materials science, or related field is required or five years experience as facilities director of a TEM facility. Expertise in electron diffraction, imaging techniques, analytical techniques, and high-resolution electron microscopy (HREM) is required. Experience working with aberration corrected TEM is preferred.
Description of the University and the Community Located 110 miles south of Portland, the University of Oregon has an enrollment of ~20,000 students. The Eugene metro area (pop. 215,000) is in a region noted for its dynamic quality of life and progressive cultural environment. We are about an hour's drive from the Pacific coast and the crest of the Cascade Mountains. The University is an AAU research institution and a member of the Pac-10 conference.
Commitment to Affirmative Action and Equal Opportunity The successful candidate will have the ability to work effectively with faculty, staff and students from a variety of diverse backgrounds.
Application Procedure and Closing Dates Applicants should submit a cover letter, curriculum vitae and arrange for three reference letters to be sent electronically to Carol Hanson at carolhan-at-uoregon.edu. Review will begin 3/20/09 and position will remain open until filled.
JOB DESCRIPTION
The position of Director for the CAMCOR TEM Facility requires a high energy, responsible, efficient and meticulous manager/analyst that is focused on ensuring that the facility is reliably operational and available to all faculty, students and commercial users on a daily basis. The Director is required to manage the facility budget, ensure that a sound business model is implemented that provides the necessary income level sufficient to cover operating costs and salaries and provide vision for the facility. The director will need to know how to get many types ofTEM data, understand what types of TEM data are most useful for specific problems, and work with professors and industries from different disciplines and sell the use of TEM as important to their work.
The main areas of responsibility are: Technical, Management, Training, Budgetary, Promotion, Scientific, Education, Operational, Documentation, Service and Cooperation. The following are some specific examples of these: 1. Technical - Familiar with all procedures and techniques - Operate all instruments and equipment professionally
2. Management - Oversee budget and purchases - Student and staff employees
3. Training - Train student, staff and instrument and laboratory users - Assist faculty and other users
4. Promotion - Improve lab visibility though various mechanisms - Workshops - Web pages - Commercial visits and lectures
7. Operational - Ensure day to day operational reliability and staffing
8. Documentation - Keep log and sample notebooks - Write lecture notes, training and reference manuals - Technical notes
9. Service - Provide "fee for service" for commercial work - Provide "public" access - Provide tours and guided explanations to misc visitors other UofO classes
10. Cooperation - Work with faculty to determine technical goals, write new instrumentation proposals, recruit new faculty and graduate students
Specific examples: 1. Ensure that state-of-the-art instrumentation is available and replaced on a cyclical basis by organizing the design and writing of government and private grant proposals. This requires working with faculty to identify analytical needs of the UofO faculty research programs that are suitable for a multi-user facility and ideally offer a capability that other four year colleges and commercial interests can also utilize, to provide additional outside income. 2. Design instrumentation specification and performance guidelines for instrument purchases. Work with faculty and the UofO business office and legal office to obtain scientific instrumentation that is optimized for UofO research needs and pushes the vendor engineering to the current limit of ultimate performance. At the same time, work with multiple vendors to obtain the lowest possible cost to UofO. 3. Manage and train both part-time students and full-time staff to ensure that all analytical practices are adhered to in a rigorous and quantitative manner. Ensure that all procedures are properly documented and available. 4. Manage the daily operation of the facility by ensuring that all consumables and supplies are freely available and ready for immediate usage. See that all computers, scanners, microscopes and tools are supplied and updated as necessary. 5. Ensure that all software applications for both data and image acquisition and also off-line reprocessing are specified and configured to provide the maximum efficiency and ease of use for the best possible utility. Work with all software vendors to implement improvements and suggestions in the accuracy, usability and technical aspects of the software. 6. Design and teach courses in the relevant technical areas of facility to promote students' theoretical and practical understanding of transmission electron microscopy (TEM), electron diffraction, imaging techniques, analytical techniques, and high-resolution electron microscopy (HREM). Update and publish lecture notes, research papers and technical "whitepapers" to improve and advance knowledge in the field. 7. Attend conferences and give presentations and posters to promote the facility and also to learn new advances in the field and new ideas that might prove useful to the facility. Maintain professional contacts with other laboratory managers and exchange ideas and technical know-how. 8. Ensure that all instruments are properly serviced, maintained and upgraded or improved if possible. Work with UofO CAMCOR and TSA service technicians to ensure that the instruments are given priority repairs and regular maintenance to provide maximum reliability. 9. Implement automation procedures for all data acquisition to the maximum extent possible to ensure round the clock instrument usage. This may require working with OEM and third party software vendors to negotiate new capabilities in the application software to achieve this goal. 10. Promote, advertise and circulate literature and "glossy" brochures to all interested UofO and OUS faculty in general to maintain maximum utilization of the instrument schedule. Maintain a web-based user schedule for users to improve scheduling efficiency. 11. Maintain supplies and equipment for efficient sample. 12. Operate all instruments for commercial fee for service usage as scheduled or on an emergency basis for time critical work. Process all data to highest levels of professional skill and accuracy. Prepare documentation of all procedures, work, analysis configuration and acquisition and data reprocessing providing precision estimates and accuracy to the best of ability. Ensure that all commercial samples and data are protected and secured.
==============================Original Headers============================== 25, 20 -- From donovan-at-uoregon.edu Thu Feb 19 18:12:11 2009 25, 20 -- Received: from smtp.uoregon.edu (mserv3.uoregon.edu [128.223.142.101]) 25, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K0CArZ030200 25, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 18:12:10 -0600 25, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 25, 20 -- (authenticated bits=0) 25, 20 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n1K0C9h5018158 25, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 25, 20 -- for {microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 16:12:10 -0800 25, 20 -- Message-Id: {200902200012.n1K0C9h5018158-at-smtp.uoregon.edu} 25, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 25, 20 -- Date: Thu, 19 Feb 2009 16:11:54 -0800 25, 20 -- To: microscopy-at-microscopy.com 25, 20 -- From: John Donovan {donovan-at-uoregon.edu} 25, 20 -- Subject: Position Open: Director, CAMCOR Transmission Electron 25, 20 -- Microscopy Facility, University of Oregon, Eugene, Oregon 25, 20 -- Mime-Version: 1.0 25, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 25, 20 -- X-Virus-Scanned: ClamAV 0.94.2/9015/Thu Feb 19 10:30:06 2009 on mserv3 25, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
X-from your question it appears you are more advanced than us when it comes to studying hair specimens. We receive one or two hair specimens per year so our experience is quite limited. We don't follow any diagnostic algorithm and I can't comment on your question about telogen effluvium. Maybe someone else here on the forum can help. We examine the hair shaft and cuticle for structural defects by SEM and examine the hair shaft through polarised light by LM. Other than that we don't have any advanced methodology.
Regards,
John Brealey Supervising Scientist EM Unit Queen Elizabeth Hospital SA Pathology South Australia
Hi John
It was interesting to follow your comments on the Microscopy Listserver as we are in a similar position here at Pathology Queensland. At present out pathologist is requesting Whole Mount for polarised LM and then SEM on every specimen that walks through the door.
I was curious to know if you follow a diagnostic algorithm for Hair specimens. Eg for Telogen Effluvium is it considered necessary to go to SEM or just tally ratio of root bulbs at LM?
Thank you for your contributions Naomi
Naomi McCallum Supervising Scientist Electron Microscope Unit Anatomical Pathology & Cytopathology PATHOLOGY QUEENSLAND Central Laboratory RBWH
==============================Original Headers============================== 8, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 19:23:44 2009 8, 27 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K1Nhk2013286 8, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 19:23:44 -0600 8, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000"; 8, 27 -- d="scan'208";a="11377363" 8, 27 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88]) 8, 27 -- by mailgate8.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 11:53:42 +1030 8, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 8, 27 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id 8, 27 -- 8.1.263.0; Fri, 20 Feb 2009 11:54:32 +1030 8, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 8, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 87A8F34C34 for 8, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 11:53:42 +1030 (CST) 8, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 8, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 8, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 27 -- Subject: SEM of Hair 8, 27 -- Date: Fri, 20 Feb 2009 11:53:43 +1030 8, 27 -- Organization: IMVS 8, 27 -- Message-ID: {000001c992f9$de8af350$7d8a140a-at-41347i} 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; charset="us-ascii" 8, 27 -- Content-Transfer-Encoding: 7bit 8, 27 -- X-Mailer: Microsoft Office Outlook 11 8, 27 -- Thread-Index: AcmS+d5kqvxuZq0JRruEkNNJHrRNMA== 8, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
Hi Kristen, That's a good exercise to do. It's more interesting when you can be the guinea pig, ie, when you examine your own hair. Our method is really basic - we cut the hair with a sharp blade and carefully place the pieces on a double-sided sticky tab that is stuck to an SEM stub. Then we coat with gold in a sputter coater.
Regards,
John
Hi John,
I've been following this a bit and am a total novice in examining hair samples with SEM. I'm always looking for good exercise for my undergrad students to do with SEM. Would you mind sharing how you prep the samples for SEM?
Thanks, Kristen
--- On Thu, 2/19/09, john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au} wrote:
} From: john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au} } Subject: [Microscopy] SEM of Hair } To: kamlennon-at-yahoo.com } Date: Thursday, February 19, 2009, 7:30 PM } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Naomi, } } X-from your question it appears you are more advanced than us when it } comes to studying hair specimens. We receive one or two hair } specimens per year so our experience is quite limited. } We don't follow any diagnostic algorithm and I can't comment on your } question about telogen effluvium. Maybe someone else here on the } forum can help. } We examine the hair shaft and cuticle for structural defects by SEM } and examine the hair shaft through polarised light by LM. } Other than that we } don't have any advanced methodology. } } Regards, } } John Brealey } Supervising Scientist } EM Unit } Queen Elizabeth Hospital } SA Pathology } South Australia } } } Hi John } } It was interesting to follow your comments on the Microscopy } Listserver as we are in a similar position here at Pathology } Queensland. } At present out } pathologist is requesting Whole Mount for polarised LM and then SEM on } every specimen that walks through the door. } } I was curious to know if you follow a diagnostic algorithm for Hair } specimens. Eg for Telogen Effluvium is it considered necessary to go } to SEM or just tally ratio of root bulbs at LM? } } Thank you for your contributions } Naomi } } Naomi McCallum } Supervising Scientist } Electron Microscope Unit } Anatomical Pathology & Cytopathology } PATHOLOGY QUEENSLAND } Central Laboratory } RBWH } }
==============================Original Headers============================== 10, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 20:00:16 2009 10, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K20Ep0027815 10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 20:00:15 -0600 10, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000"; 10, 27 -- d="scan'208";a="11464458" 10, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 10, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 12:30:14 +1030 10, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 10, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 10, 27 -- 8.1.263.0; Fri, 20 Feb 2009 12:29:21 +1030 10, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 10, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 1D5CC34007 for 10, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 12:30:14 +1030 (CST) 10, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 10, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 10, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 10, 27 -- Subject: SEM of Hair 10, 27 -- Date: Fri, 20 Feb 2009 12:30:13 +1030 10, 27 -- Organization: IMVS 10, 27 -- Message-ID: {000601c992fe$f8116d90$7d8a140a-at-41347i} 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; charset="us-ascii" 10, 27 -- Content-Transfer-Encoding: 7bit 10, 27 -- X-Mailer: Microsoft Office Outlook 11 10, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 10, 27 -- Thread-Index: AcmS/vf0b28prB0ZRk6i4tHimaOr7g== ==============================End of - Headers==============================
A tip that I learned from a certain SEM guru was that you get a better cross-section of the hair shaft if you fracture in liquid nitrogen. He described this technique of putting the hairs through the centre of a straw and filling the straw with liquid carbon. After freezing in Liq N2 you hit the straw with a sharp blade to fracture. There was some discussion about getting the right type of straw to make this work.
I tried this but didn't persist long enough to master it without bubbles in the straw, so I modified the technique a little. We use jewellers wire and crimps to make a 'brush' from multiple hairs (another local technique). Then we coat the hair in a sucrose blocking media used for histologic frozen sections; freeze in liq N2; fracture and then wash off the media. It works well for TS of hair shaft, you get a true fracture and not "a study in cutting techniques" (I believe that was the description).
We still use the method described below as well, mounting the LS and TS brush on the same stub.
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Kristen, That's a good exercise to do. It's more interesting when you can be the guinea pig, ie, when you examine your own hair. Our method is really basic - we cut the hair with a sharp blade and carefully place the pieces on a double-sided sticky tab that is stuck to an SEM stub. Then we coat with gold in a sputter coater.
Regards,
John
Hi John,
I've been following this a bit and am a total novice in examining hair samples with SEM. I'm always looking for good exercise for my undergrad students to do with SEM. Would you mind sharing how you prep the samples for SEM?
Thanks, Kristen
--- On Thu, 2/19/09, john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au} wrote:
} From: john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au} } Subject: [Microscopy] SEM of Hair } To: kamlennon-at-yahoo.com } Date: Thursday, February 19, 2009, 7:30 PM } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Naomi, } } X-from your question it appears you are more advanced than us when it } comes to studying hair specimens. We receive one or two hair } specimens per year so our experience is quite limited. } We don't follow any diagnostic algorithm and I can't comment on your } question about telogen effluvium. Maybe someone else here on the } forum can help. } We examine the hair shaft and cuticle for structural defects by SEM } and examine the hair shaft through polarised light by LM. } Other than that we } don't have any advanced methodology. } } Regards, } } John Brealey } Supervising Scientist } EM Unit } Queen Elizabeth Hospital } SA Pathology } South Australia } } } Hi John } } It was interesting to follow your comments on the Microscopy } Listserver as we are in a similar position here at Pathology } Queensland. } At present out } pathologist is requesting Whole Mount for polarised LM and then SEM on } every specimen that walks through the door. } } I was curious to know if you follow a diagnostic algorithm for Hair } specimens. Eg for Telogen Effluvium is it considered necessary to go } to SEM or just tally ratio of root bulbs at LM? } } Thank you for your contributions } Naomi } } Naomi McCallum } Supervising Scientist } Electron Microscope Unit } Anatomical Pathology & Cytopathology } PATHOLOGY QUEENSLAND } Central Laboratory } RBWH } }
==============================Original Headers============================== 10, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 20:00:16 2009 10, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K20Ep0027815 10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 20:00:15 -0600 10, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000"; 10, 27 -- d="scan'208";a="11464458" 10, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 10, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 12:30:14 +1030 10, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 10, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 10, 27 -- 8.1.263.0; Fri, 20 Feb 2009 12:29:21 +1030 10, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 10, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 1D5CC34007 for 10, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 12:30:14 +1030 (CST) 10, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 10, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 10, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 10, 27 -- Subject: SEM of Hair 10, 27 -- Date: Fri, 20 Feb 2009 12:30:13 +1030 10, 27 -- Organization: IMVS 10, 27 -- Message-ID: {000601c992fe$f8116d90$7d8a140a-at-41347i} 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; charset="us-ascii" 10, 27 -- Content-Transfer-Encoding: 7bit 10, 27 -- X-Mailer: Microsoft Office Outlook 11 10, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 10, 27 -- Thread-Index: AcmS/vf0b28prB0ZRk6i4tHimaOr7g== ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 26 -- From prvs=13026700a6=naomi_mccallum-at-health.qld.gov.au Thu Feb 19 22:29:34 2009 22, 26 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 22, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K4TWut012703 22, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 22:29:34 -0600 22, 26 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 22, 26 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id n1K4Sfqo004341 22, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:42 +1000 22, 26 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 22, 26 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 22, 26 -- with ESMTP id 22, 26 -- {T8ca07f5847c0a80335e00-at-gwd-mail06.remote.health.qld.gov.au} for 22, 26 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:41 +1000 22, 26 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 22, 26 -- Novell_GroupWise; Fri, 20 Feb 2009 14:28:41 +1000 22, 26 -- Message-Id: {499EBD0F.88BE.00AA.0-at-health.qld.gov.au} 22, 26 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 22, 26 -- Date: Fri, 20 Feb 2009 14:28:36 +1000 22, 26 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au} 22, 26 -- To: {Microscopy-at-microscopy.com} 22, 26 -- Subject: Fwd: [Microscopy] SEM of Hair 22, 26 -- References: {200902200206.n1K26CgH008879-at-ns.microscopy.com} 22, 26 -- Mime-Version: 1.0 22, 26 -- Content-Type: text/plain; charset="us-ascii" 22, 26 -- Content-Disposition: inline 22, 26 -- Content-Transfer-Encoding: 8bit 22, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1K4TWut012703 ==============================End of - Headers==============================
As the SEM Guru mentioned by Naomi, I thought I could add a little to the proceedings on hair/fibres and on the earlier paper question.
Naomi has hinted at the criteria for cross sections by fracture but I will expand. The SEM is very clever; it recognises that a material was cut with a blunt scalpel blade and the difference between the cut from a blunt blade and from a sharp blade. With that in mind we know that scientists often need to quickly cross section a material with a result that is a true representation of the material how, well crack it! Some materials will crack naturally having been cooled in liquid nitrogen, but others will not; hair is a good example of a difficult material to truly fracture.
When a material will not fracture naturally you need to stiffen the material. If the material is not soluble in water we use a water soluble carbon solution as the stiffener. Drill a fine (1/8th inch) hole though two stubs placed face to face. Place your fibres/material through the hole and fill the additional space with the carbon solution. When dry plunge the unit into liquid nitrogen and when the liquid stops boiling take the unit out and crack it by striking the interface with a single edged blade. Take great care not to cut through the unit, simple crack it open.
Once the two units are back at room temperature and the condensation has dispersed they are usable in the light microscope and the SEM.
The most complex use of the technique so far was to view two types of freezer bag, one was failing once frozen! We rolled strips of each bag into a coil and fractured with support as described. Well, we found that the bags were made up of layers, 4 in the poor bag 5 in the good bag. The extra layer, even though you could see through the bag, was aluminium; interesting?
Hope this helps and by the way we use the straw technique mentioned in cryo systems only! Guru I may be but clearly in that case I have led people astray; its age!
Steve
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: naomi_mccallum-at-health.qld.gov.au [mailto:naomi_mccallum-at-health.qld.gov.au] Sent: 20 February 2009 04:31 To: protrain-at-emcourses.com
Hi all
A tip that I learned from a certain SEM guru was that you get a better cross-section of the hair shaft if you fracture in liquid nitrogen. He described this technique of putting the hairs through the centre of a straw and filling the straw with liquid carbon. After freezing in Liq N2 you hit the straw with a sharp blade to fracture. There was some discussion about getting the right type of straw to make this work.
I tried this but didn't persist long enough to master it without bubbles in the straw, so I modified the technique a little. We use jewellers wire and crimps to make a 'brush' from multiple hairs (another local technique). Then we coat the hair in a sucrose blocking media used for histologic frozen sections; freeze in liq N2; fracture and then wash off the media. It works well for TS of hair shaft, you get a true fracture and not "a study in cutting techniques" (I believe that was the description).
We still use the method described below as well, mounting the LS and TS brush on the same stub.
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Kristen, That's a good exercise to do. It's more interesting when you can be the guinea pig, ie, when you examine your own hair. Our method is really basic - we cut the hair with a sharp blade and carefully place the pieces on a double-sided sticky tab that is stuck to an SEM stub. Then we coat with gold in a sputter coater.
Regards,
John
Hi John,
I've been following this a bit and am a total novice in examining hair samples with SEM. I'm always looking for good exercise for my undergrad students to do with SEM. Would you mind sharing how you prep the samples for SEM?
Thanks, Kristen
--- On Thu, 2/19/09, john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au} wrote:
} From: john.brealey-at-imvs.sa.gov.au {john.brealey-at-imvs.sa.gov.au} } Subject: [Microscopy] SEM of Hair } To: kamlennon-at-yahoo.com } Date: Thursday, February 19, 2009, 7:30 PM } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Naomi, } } X-from your question it appears you are more advanced than us when it } comes to studying hair specimens. We receive one or two hair } specimens per year so our experience is quite limited. } We don't follow any diagnostic algorithm and I can't comment on your } question about telogen effluvium. Maybe someone else here on the } forum can help. } We examine the hair shaft and cuticle for structural defects by SEM } and examine the hair shaft through polarised light by LM. } Other than that we } don't have any advanced methodology. } } Regards, } } John Brealey } Supervising Scientist } EM Unit } Queen Elizabeth Hospital } SA Pathology } South Australia } } } Hi John } } It was interesting to follow your comments on the Microscopy } Listserver as we are in a similar position here at Pathology } Queensland. } At present out } pathologist is requesting Whole Mount for polarised LM and then SEM on } every specimen that walks through the door. } } I was curious to know if you follow a diagnostic algorithm for Hair } specimens. Eg for Telogen Effluvium is it considered necessary to go } to SEM or just tally ratio of root bulbs at LM? } } Thank you for your contributions } Naomi } } Naomi McCallum } Supervising Scientist } Electron Microscope Unit } Anatomical Pathology & Cytopathology } PATHOLOGY QUEENSLAND } Central Laboratory } RBWH } }
==============================Original Headers============================== 10, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Feb 19 20:00:16 2009 10, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K20Ep0027815 10, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 20:00:15 -0600 10, 27 -- X-IronPort-AV: E=Sophos;i="4.38,237,1233495000"; 10, 27 -- d="scan'208";a="11464458" 10, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 10, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Feb 2009 12:30:14 +1030 10, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 10, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 10, 27 -- 8.1.263.0; Fri, 20 Feb 2009 12:29:21 +1030 10, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 10, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 1D5CC34007 for 10, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 12:30:14 +1030 (CST) 10, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 10, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 10, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 10, 27 -- Subject: SEM of Hair 10, 27 -- Date: Fri, 20 Feb 2009 12:30:13 +1030 10, 27 -- Organization: IMVS 10, 27 -- Message-ID: {000601c992fe$f8116d90$7d8a140a-at-41347i} 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; charset="us-ascii" 10, 27 -- Content-Transfer-Encoding: 7bit 10, 27 -- X-Mailer: Microsoft Office Outlook 11 10, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 10, 27 -- Thread-Index: AcmS/vf0b28prB0ZRk6i4tHimaOr7g== ==============================End of - Headers==============================
**************************************************************************** **** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************************** ******
==============================Original Headers============================== 22, 26 -- From prvs=13026700a6=naomi_mccallum-at-health.qld.gov.au Thu Feb 19 22:29:34 2009 22, 26 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 22, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K4TWut012703 22, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Feb 2009 22:29:34 -0600 22, 26 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 22, 26 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id n1K4Sfqo004341 22, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:42 +1000 22, 26 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 22, 26 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 22, 26 -- with ESMTP id 22, 26 -- {T8ca07f5847c0a80335e00-at-gwd-mail06.remote.health.qld.gov.au} for 22, 26 -- {Microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 14:28:41 +1000 22, 26 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 22, 26 -- Novell_GroupWise; Fri, 20 Feb 2009 14:28:41 +1000 22, 26 -- Message-Id: {499EBD0F.88BE.00AA.0-at-health.qld.gov.au} 22, 26 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 22, 26 -- Date: Fri, 20 Feb 2009 14:28:36 +1000 22, 26 -- From: "Naomi Mccallum" {naomi_mccallum-at-health.qld.gov.au} 22, 26 -- To: {Microscopy-at-microscopy.com} 22, 26 -- Subject: Fwd: [Microscopy] SEM of Hair 22, 26 -- References: {200902200206.n1K26CgH008879-at-ns.microscopy.com} 22, 26 -- Mime-Version: 1.0 22, 26 -- Content-Type: text/plain; charset="us-ascii" 22, 26 -- Content-Disposition: inline 22, 26 -- Content-Transfer-Encoding: 8bit 22, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1K4TWut012703 ==============================End of - Headers==============================
==============================Original Headers============================== 37, 27 -- From protrain-at-emcourses.com Fri Feb 20 03:29:09 2009 37, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 37, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1K9T8VF002380 37, 27 -- for {microscopy-at-microscopy.com} ; Fri, 20 Feb 2009 03:29:08 -0600 37, 27 -- Received: from 5ac8bf20.bb.sky.com ([90.200.191.32]:4756 helo=HP6220) 37, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 37, 27 -- (envelope-from {protrain-at-emcourses.com} ) 37, 27 -- id 1LaRgs-0005Bw-JQ by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 20 Feb 2009 11:29:03 +0200 37, 27 -- Reply-To: {protrain-at-emcourses.com} 37, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 37, 27 -- To: {naomi_mccallum-at-health.qld.gov.au} 37, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 37, 27 -- References: {200902200430.n1K4UYTl014110-at-ns.microscopy.com} 37, 27 -- Subject: RE: [Microscopy] Fwd: SEM of Hair 37, 27 -- Date: Fri, 20 Feb 2009 09:28:41 -0000 37, 27 -- Organization: Protrain 37, 27 -- Message-ID: {003901c9933d$ad5b4de0$0200a8c0-at-HP6220} 37, 27 -- MIME-Version: 1.0 37, 27 -- Content-Type: text/plain; 37, 27 -- charset="us-ascii" 37, 27 -- Content-Transfer-Encoding: 7bit 37, 27 -- X-Mailer: Microsoft Office Outlook 11 37, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 37, 27 -- Thread-Index: AcmTFAWjDrrRW1vERqyTtsCF/XOxsAAJgpIg 37, 27 -- In-Reply-To: {200902200430.n1K4UYTl014110-at-ns.microscopy.com} 37, 27 -- X-Scan-Signature: e61ed4b52b61ed9601f755c09be3f9a3{293}} 37, 27 -- X-Trace: smtp01.dial-up.net 1LaRgs-0005Bw-JQ 6cb117f5a30fc6b35b332f22f9d3c1ea ==============================End of - Headers==============================
Maybe a awkward question, but I'm not chemist : what do you call "a water soluble carbon solution" or "liquid carbon" ?
Do you think at carbon paint, something like Leit C or a suspension of carbon black in water ?
Jacques
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
I have a researcher who will be wanting to cut ultrathin sections of fossil tooth pieces (size ~ 3x5mm) for TEM observation.
Since most of my TEM experience come from plant materials, I'm wondering if there are some special requirement/tricks for sample preparation (e.g. embedding), microtome (knife selection, cryo or RT) and post-staining etc.
I googled the internet but could not find much info/protocols to follow. Any suggestion and advice are greatly appreciated.
Thanks in advance.
Guosheng Dept of Biology U of Saskatchewan Canada
==============================Original Headers============================== 6, 20 -- From gul417-at-mail.usask.ca Fri Feb 20 10:17:05 2009 6, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1KGH2Sc014574 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Feb 2009 10:17:04 -0600 6, 20 -- Received: from conversion-daemon.usask.ca by usask.ca 6, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007)) 6, 20 -- id {0KFD00C01GXVCQ-at-usask.ca} (original mail from gul417-at-mail.usask.ca) 6, 20 -- for Microscopy-at-Microscopy.Com; Fri, 20 Feb 2009 10:16:16 -0600 (CST) 6, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31]) 6, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007)) 6, 20 -- with SMTP id {0KFD00KGRH7479-at-usask.ca} for Microscopy-at-Microscopy.Com; Fri, 6, 20 -- 20 Feb 2009 10:16:16 -0600 (CST) 6, 20 -- Date: Fri, 20 Feb 2009 10:16:16 -0600 (CST) 6, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca} 6, 20 -- Subject: TEM of fossil tooth 6, 20 -- To: Microscopy-at-Microscopy.Com 6, 20 -- Message-id: {2208155.1235146576267.JavaMail.gul417-at-mail.usask.ca} 6, 20 -- MIME-version: 1.0 6, 20 -- Content-type: text/plain; charset=UTF-8 6, 20 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
Use a FIB with lift-out. Especially if the fossils are valuable and you don't want to consume the tooth in making a TEM sample.
John Mardinly, Numonyx
-----Original Message----- X-from: gul417-at-mail.usask.ca [mailto:gul417-at-mail.usask.ca] Sent: Friday, February 20, 2009 8:37 AM To: MARDINLY, A
Dear All,
I have a researcher who will be wanting to cut ultrathin sections of fossil tooth pieces (size ~ 3x5mm) for TEM observation.
Since most of my TEM experience come from plant materials, I'm wondering if there are some special requirement/tricks for sample preparation (e.g. embedding), microtome (knife selection, cryo or RT) and post-staining etc.
I googled the internet but could not find much info/protocols to follow. Any suggestion and advice are greatly appreciated.
Thanks in advance.
Guosheng Dept of Biology U of Saskatchewan Canada
==============================Original Headers============================== 6, 20 -- From gul417-at-mail.usask.ca Fri Feb 20 10:17:05 2009 6, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1KGH2Sc014574 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Feb 2009 10:17:04 -0600 6, 20 -- Received: from conversion-daemon.usask.ca by usask.ca 6, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007)) 6, 20 -- id {0KFD00C01GXVCQ-at-usask.ca} (original mail from gul417-at-mail.usask.ca) 6, 20 -- for Microscopy-at-Microscopy.Com; Fri, 20 Feb 2009 10:16:16 -0600 (CST) 6, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31]) 6, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007)) 6, 20 -- with SMTP id {0KFD00KGRH7479-at-usask.ca} for Microscopy-at-Microscopy.Com; Fri, 6, 20 -- 20 Feb 2009 10:16:16 -0600 (CST) 6, 20 -- Date: Fri, 20 Feb 2009 10:16:16 -0600 (CST) 6, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca} 6, 20 -- Subject: TEM of fossil tooth 6, 20 -- To: Microscopy-at-Microscopy.Com 6, 20 -- Message-id: {2208155.1235146576267.JavaMail.gul417-at-mail.usask.ca} 6, 20 -- MIME-version: 1.0 6, 20 -- Content-type: text/plain; charset=UTF-8 6, 20 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
==============================Original Headers============================== 15, 29 -- From A.MARDINLY-at-numonyx.com Fri Feb 20 14:28:38 2009 15, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1KKScgY006822 15, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 20 Feb 2009 14:28:38 -0600 15, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 15, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id A682624801A; 15, 29 -- Fri, 20 Feb 2009 14:50:28 -0500 (EST) 15, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 15, 29 -- Fri, 20 Feb 2009 15:28:37 -0500 15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 29 -- Content-class: urn:content-classes:message 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; 15, 29 -- charset="us-ascii" 15, 29 -- Subject: RE: [Microscopy] TEM of fossil tooth 15, 29 -- Date: Fri, 20 Feb 2009 15:28:09 -0500 15, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9E19622-at-EXDRESBENMX012.numonyx.local} 15, 29 -- In-Reply-To: {200902201636.n1KGaiIM026897-at-ns.microscopy.com} 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- Thread-Topic: [Microscopy] TEM of fossil tooth 15, 29 -- Thread-Index: AcmTeXWAbT4oTPQ/S4GosfWMMsXn5AAIBIDg 15, 29 -- References: {200902201636.n1KGaiIM026897-at-ns.microscopy.com} 15, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 15, 29 -- To: {gul417-at-mail.usask.ca} 15, 29 -- Cc: {Microscopy-at-Microscopy.com} 15, 29 -- X-OriginalArrivalTime: 20 Feb 2009 20:28:37.0884 (UTC) FILETIME=[CF972BC0:01C99399] 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1KKScgY006822 ==============================End of - Headers==============================
Hello all, I just got an ISI ABT-55, and while the manual doesn't look too different from the SX40 I've got, it looks different enough to warrant getting an English copy. You see, the manual I have is entirely in Japanese. I will, of course, offer the Japanese version as trade for a copy of an English version... The perfect gift for anyone looking to learn another language!
Also, if anybody has one of these that is mothballed or headed that way, please contact me off list, I am in need of a few ABT-55 specific odds and ends.
Thanks,
Justin.
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 5, 31 -- From kraftpiano-at-gmail.com Sat Feb 21 15:48:20 2009 5, 31 -- Received: from mail-qy0-f20.google.com (mail-qy0-f20.google.com [209.85.221.20]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1LLmKWl018696 5, 31 -- for {microscopy-at-microscopy.com} ; Sat, 21 Feb 2009 15:48:20 -0600 5, 31 -- Received: by qyk13 with SMTP id 13so2333571qyk.18 5, 31 -- for {microscopy-at-microscopy.com} ; Sat, 21 Feb 2009 13:48:20 -0800 (PST) 5, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 31 -- d=gmail.com; s=gamma; 5, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject 5, 31 -- :from:to:content-type:content-transfer-encoding; 5, 31 -- bh=CjJPGi0F2aPEDlVLb952S0xu74Zlf2uvALyf04bxtdw=; 5, 31 -- b=g3hX3iFonGU4NL1PKbWaFcquQ6xOe2uEVBZqU7DW8J24PLNMEGOT6bzcEH/yn951p/ 5, 31 -- LRTygztVPcea9sgSNVKmJxYN85lSwO+/LHg7AjclK5T7+dtB+QlrWmrLzU+VuktWp49T 5, 31 -- EQ4+zTYhG8QWPYnkBdcm/2HvB4VjA5vqM2UVU= 5, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 31 -- d=gmail.com; s=gamma; 5, 31 -- h=mime-version:date:message-id:subject:from:to:content-type 5, 31 -- :content-transfer-encoding; 5, 31 -- b=xUaZBJtQWSXrH+Tc34DmPTpqAtQoRotJLH1eSjP1PIpnjc+Jbmnktq5Ia64OBlLEEx 5, 31 -- OUB4YNQlJM1JPzmAG1aZOwQh6ATNsO7QoVvRBxAhtc9hmZNW+MUwfNCuDCfTkHe7R9Sb 5, 31 -- XD9VFvyecJRlIveHStDOc9xZFKb4VRgkQKFAs= 5, 31 -- MIME-Version: 1.0 5, 31 -- Received: by 10.224.37.17 with SMTP id v17mr3699273qad.128.1235252900403; Sat, 5, 31 -- 21 Feb 2009 13:48:20 -0800 (PST) 5, 31 -- Date: Sat, 21 Feb 2009 16:48:19 -0500 5, 31 -- Message-ID: {25e2b0d20902211348k27dd23a5mf8b3d39f80cccd6c-at-mail.gmail.com} 5, 31 -- Subject: Looking for manual & parts... 5, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com} 5, 31 -- To: microscopy-at-microscopy.com 5, 31 -- Content-Type: text/plain; charset=ISO-8859-1 5, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slc6-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh Microscopy School
Title-Subject: [Filtered] Lehigh Microscopy School Courses
Question: There is still time to register for the 2009 Lehigh Microscopy School which will be held May 31-June 12, 2009. This will be the 39th year of course offerings which include: SEM and X-ray Microanalysis (May 31-June 5), Introduction to SEM and EDS for the New Operator (May 31), Scanning Probe Microscopy: From Fundamentals to Advanced Applications (June 8-11), Problem Solving with SEM, X-ray Microanalysis, and Electron Backscatter Patterns (June 8-12), Quantitative X-ray Microanalysis: Problem Solving using EDS and WDS Techniques (June 8-12), Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications (June 8-11), Focused Ion Beam (FIB) Instrumentation and Applications (June 8-11). Complete course descriptions and registration form are available at www.Lehigh.edu/microscopy. Contact Sharon Coe (Sharon.coe-at-Lehigh.edu) for more information.
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Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of South Carolina
Title-Subject: [Filtered] 3d reconstruction software
Question: Is there a software that most people are using to reconstruct serial sections both at the em and lm levels? I used on once maybe 10 years ago but don't remember who developed it.
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Email: srijay-at-stanford.edu Name: Jay
Organization: Stanford University
Title-Subject: [Filtered] processing nerve cells on ACLAR for TEM
Question: Hi,
I have a couple of questions-
1. What is the best way to sterilise Aclar discs to grow nerve cells.
2. After infiltration step how can the discs be embedded for thin sectioning?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton University
Title-Subject: [Filtered] Tannic Acid
Question: Is it possible that tannic acid - the powder itself - in a plastic jar, can expire?
We are having some issues with getting good contrast. I have been following a published protocol for imaging cilia in the kidneys of zebrafish.
Just trying to systematically see if there is something different that we are doing and wondering if it could be that bottle of tannic acid on the shelf.
The type of tannic acid makes a big difference. There are low and high molecular weight forms depending on its source. Go the EMS website and the technical data sheet for their tannic acid has a nice description of this issue and literature citations. (No commercial interest - just a happy user). Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: mbisher-at-princeton.edu [mailto:mbisher-at-princeton.edu] Sent: Monday, February 23, 2009 5:11 PM To: Phillips, Thomas E.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both mbisher-at-princeton.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton University
Title-Subject: [Filtered] Tannic Acid
Question: Is it possible that tannic acid - the powder itself - in a plastic jar, can expire?
We are having some issues with getting good contrast. I have been following a published protocol for imaging cilia in the kidneys of zebrafish.
Just trying to systematically see if there is something different that we are doing and wondering if it could be that bottle of tannic acid on the shelf.
Announcement of Senior Engineer/Scientist opening at Schafer Corporation.
Schafer Vallecitos Laboratory is seeking a Senior Engineer / Scientist to add to our mass spectrometry group. SVL performs materials characterization and related analytical services on commercial and government contracts. The activities of the group include chemical and elemental analysis of materials on a production basis, maintenance of several mass spectrometers and ancillary equipment, development of new or improved MS analysis techniques, and quality assurance of analytical data.
Responsibilities: The Senior Engineer / Scientist will provide theoretical and practical knowledge in the design, operation and maintenance of secondary ion mass spectrometry (SIMS) instruments and analysis of the data they produce.
For details on this opening, see http://jobs-schafer.icims.com/jobs/1515/job (Do not reply to this message.)
==============================Original Headers============================== 5, 19 -- From mzemyan-at-schaferlabs.com Tue Feb 24 13:02:52 2009 5, 19 -- Received: from mail.schaferlabs.com (mx1.schaferlabs.com [64.168.91.154]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1OJ2nEI004055 5, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 13:02:51 -0600 5, 19 -- Received: from mzemyanws ([10.10.10.1]) 5, 19 -- by mail.schaferlabs.com (8.12.11.20060308/8.12.11) with ESMTP id n1OJ2lUp027339 5, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 11:02:47 -0800 5, 19 -- Message-Id: {200902241902.n1OJ2lUp027339-at-mail.schaferlabs.com} 5, 19 -- From: "Mike Zemyan" {mzemyan-at-schaferlabs.com} 5, 19 -- To: {Microscopy-at-microscopy.com} 5, 19 -- Subject: SIMS engineer/scientist opening at Schafer Corporation 5, 19 -- Date: Tue, 24 Feb 2009 11:02:48 -0800 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: text/plain; 5, 19 -- charset="us-ascii" 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 5, 19 -- thread-index: AcmWsnwjAXc2sjAQRYSuluTi3XIkZw== 5, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
I have a number of very pricey aperture strips that need cleaned. The best way would be with a plasma cleaner. Does anyone have one who would be willing to try to clean a strip or can you recommend somewhere that I could send the strips for cleaning?
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 5, 29 -- From dsherman-at-purdue.edu Tue Feb 24 16:03:16 2009 5, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1OM3FhK023718 5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 16:03:15 -0600 5, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 5, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n1OM3FOG011542 5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 17:03:15 -0500 5, 29 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 5, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n1OM3FFo025403 5, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 17:03:15 -0500 5, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 29 -- Tue, 24 Feb 2009 17:03:14 -0500 5, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 5, 29 -- Tue, 24 Feb 2009 22:02:42 +0000 5, 29 -- User-Agent: Microsoft-Entourage/12.15.0.081119 5, 29 -- Date: Tue, 24 Feb 2009 17:02:40 -0500 5, 29 -- Subject: Cleaning aperture strip 5, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 5, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 5, 29 -- Message-ID: {C5C9D8B0.3B1D1%dsherman-at-exchange.purdue.edu} 5, 29 -- Thread-Topic: Cleaning aperture strip 5, 29 -- Thread-Index: AcmWy5w0YbLFc2iDQwmDVw4N2g/D8Q== 5, 29 -- Mime-version: 1.0 5, 29 -- Content-type: text/plain; 5, 29 -- charset="US-ASCII" 5, 29 -- Content-transfer-encoding: 7bit 5, 29 -- X-OriginalArrivalTime: 24 Feb 2009 22:03:14.0670 (UTC) FILETIME=[B0DEF8E0:01C996CB] 5, 29 -- X-PMX-Version: 5.4.0.320885 5, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Anyone interested in a temporary clinical TEM job?
Reply to April, see e-mail below.
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
--- On Tue, 2/24/09, April Sachau {asachau-at-titanmed.com} wrote:
} From: April Sachau {asachau-at-titanmed.com} } Subject: RE: [Histonet] Electron Microscopy } To: vapatpxs-at-yahoo.com } Date: Tuesday, February 24, 2009, 8:38 PM } I am working with a hospital here in Nebraska, and their EM } histo tech } has just left for an emergency maternity leave. They need } some full } time coverage while she is gone. Do you know of anyone } that might be } willing to work out of town for 3-4 months??? I would be } able to pay } for housing, travel...etc along with a competitive salary. } I appreciate } your help! (Also, I have been approved to give anyone } referring a } qualified individual a $250.00 referral fee!) } } } April Sachau } Titan Medical Group } Staff Supervisor } Phone (866) 332-9600 Ext. 1023 } Fax (402) 332-5181 } asachau-at-titanmed.com } } see us on the web at www.titanmed.com } } -----Original Message----- } From: Va Paula Sicurello [mailto:vapatpxs-at-yahoo.com] } Sent: Tuesday, February 24, 2009 11:44 AM } To: April Sachau } Subject: Re: [Histonet] Electron Microscopy } } Hi April, } } I have extensive experience with TEM and SEM. } } You can call me at the number below. } } Paula Sicurello } VA Medical Center San Diego } Veterans Medical Research Foundation (VMRF) } Core Microscope Facility, room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } --- On Tue, 2/24/09, April Sachau } {asachau-at-titanmed.com} wrote: } } } From: April Sachau {asachau-at-titanmed.com} } } Subject: [Histonet] Electron Microscopy } } To: histonet-at-lists.utsouthwestern.edu } } Date: Tuesday, February 24, 2009, 5:04 PM } } Hello Histoland! } } } } I am seeking an individual with Electron Microscopy } } experience... please } } contact me if you have it or know of someone that } does. } } Thanks! } } } } } } April Sachau } } Titan Medical Group } } Staff Supervisor } } Phone (866) 332-9600 Ext. 1023 } } Fax (402) 332-5181 } } asachau-at-titanmed.com } } } } see us on the web at www.titanmed.com } } } } _______________________________________________ } } Histonet mailing list } } Histonet-at-lists.utsouthwestern.edu } } } http://lists.utsouthwestern.edu/mailman/listinfo/histonet
==============================Original Headers============================== 10, 26 -- From vapatpxs-at-yahoo.com Tue Feb 24 16:03:51 2009 10, 26 -- Received: from n67.bullet.mail.sp1.yahoo.com (n67.bullet.mail.sp1.yahoo.com [98.136.44.47]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1OM3pZh024205 10, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 16:03:51 -0600 10, 26 -- Received: from [69.147.84.145] by n67.bullet.mail.sp1.yahoo.com with NNFMP; 24 Feb 2009 21:55:00 -0000 10, 26 -- Received: from [68.142.194.243] by t8.bullet.mail.sp1.yahoo.com with NNFMP; 24 Feb 2009 21:54:59 -0000 10, 26 -- Received: from [68.142.201.68] by t1.bullet.mud.yahoo.com with NNFMP; 24 Feb 2009 21:54:59 -0000 10, 26 -- Received: from [127.0.0.1] by omp420.mail.mud.yahoo.com with NNFMP; 24 Feb 2009 21:54:59 -0000 10, 26 -- X-Yahoo-Newman-Property: ymail-3 10, 26 -- X-Yahoo-Newman-Id: 781934.61652.bm-at-omp420.mail.mud.yahoo.com 10, 26 -- Received: (qmail 44816 invoked by uid 60001); 24 Feb 2009 21:54:59 -0000 10, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 26 -- s=s1024; d=yahoo.com; 10, 26 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 26 -- b=o3kFtHPrePKfshrsTbTurONZNb4/0/Pk8uROC2UIIfz+s+lKxGB4BxcIhnchSPjswQt2maimYvoTxxC+qyQrlvFVU316IzWTEqMXhTIKVXmH7dEogtUE2ze0yJddOhiAHS+CN2B2uhepnRyz1TC2v/QhwIoKM8jsIQ4LQVaT970=; 10, 26 -- X-YMail-OSG: 4Q.NNHYVM1lT46sNf3CHX8GpgGcG363lFkk0v48qPCyl8Yv.yMBxyOcGM9j2snc5a43DlGYfe3Yn3vIeBkN09KMDuh1UytYd9OQhoBUVpJ2zImjPfu4tgafzynDHj5VTEhK6hfU8Aw40CDxiOVfsVtg6V9WbTl89a0_nqeC6zE2UC_E22Ok65C0mUqm6dl3Gxc.jZmFB6boEkvc- 10, 26 -- Received: from [132.239.85.26] by web46114.mail.sp1.yahoo.com via HTTP; Tue, 24 Feb 2009 13:54:59 PST 10, 26 -- X-Mailer: YahooMailWebService/0.7.260.1 10, 26 -- Date: Tue, 24 Feb 2009 13:54:59 -0800 (PST) 10, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 26 -- Reply-To: vapatpxs-at-yahoo.com 10, 26 -- Subject: Temporary Clinical Electron Microscopy Job 10, 26 -- To: MSA BB {Microscopy-at-microscopy.com} 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; charset=us-ascii 10, 26 -- Message-ID: {248525.44422.qm-at-web46114.mail.sp1.yahoo.com} ==============================End of - Headers==============================
I am looking for recommendations for AFM systems to image wet DNA and other bio-polymers.
We are looking for a complete system. Experienced users and vendors are welcome to respond with particulars (capabilities, pricing, advantages over competiting systems, etc.)
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: abrun-at-hsc.unt.edu Name: Anne-Marie Brun
Organization: UNT HSC at Fort Worth Texas 76107, USA
Title-Subject: [Filtered] Epon/PAS stain
Question: Has anyone ever stained for PAS on Epon sections before? If not on Epon then what type of plastic did you use to do a PAS stain? I know it is done on paraffin embedded material, but an investigator wants the PAS staining done on Epon sections. ----? Thanks for your help Anne-Marie
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Title-Subject: [Filtered] broken window on EDX detector
Question: I understand that once a 'window' on an EDX detector is broken there is no way to repair it and the crystal is destroyed. Is this true and does this apply to all types of detectors?
Uh...yes. If the window is fractured, then you are SOL. This means that you need to get the detector repaired. This is not an insignificant cost. But it makes the difference working and not working.
Why did the window fail? This usually happens if the chamber is vented too quickly or for some other actions that are detrimental to the window.
The "standard" windows are MoxD and are .3u thick. These are classified as SUTW (Super Ultra Thin Window) and are sourced from UT.
I would strongly suggest that you look into why the window/film failed. You must not repeat this scenario.
gary g.
At 07:31 PM 2/24/2009, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Tue Feb 24 22:58:39 2009 9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1P4wbfi004513 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 22:58:38 -0600 9, 20 -- Message-Id: {200902250458.n1P4wbfi004513-at-ns.microscopy.com} 9, 20 -- Received: (qmail 15402 invoked from network); 24 Feb 2009 20:56:59 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 15395, pid: 15398, t: 0.1083s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by smtp2 with SMTP; 24 Feb 2009 20:56:59 -0800 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Tue, 24 Feb 2009 20:58:30 -0800 9, 20 -- To: analytic-at-rawbw.com 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: broken window on EDX detector 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200902250331.n1P3V9uG012757-at-ns.microscopy.com} 9, 20 -- References: {200902250331.n1P3V9uG012757-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
We are having problems with our XL30, where the screen freezes and turns red! Has anyone had similar problems and found what caused it??? We think it is the graphics card, which after re-seating it, the problem will go away for about a week, then it returns! We have been told we can get a new card for AUD 15,000, but thought I would see if anyone had any other ideas first. Does anyone have an old XL30 computer they could let us have the cards out of??
Thanks
Steve Parry Centre for Microscopy, Characterisation & Analysis, (M010) The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
What???? Why not replace the video card? I assume that you do not have a maintenance contract.
If you are using or stuck at WinNT, it is crappy. What are you running for the OS?
gary g.
At 09:34 PM 2/24/2009, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Tue Feb 24 23:57:07 2009 11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1P5v5m5000606 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 23:57:06 -0600 11, 20 -- Message-Id: {200902250557.n1P5v5m5000606-at-ns.microscopy.com} 11, 20 -- Received: (qmail 6254 invoked from network); 24 Feb 2009 22:00:36 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 6251, pid: 6252, t: 0.1035s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp1 with SMTP; 24 Feb 2009 22:00:36 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Tue, 24 Feb 2009 21:57:00 -0800 11, 20 -- To: steve.parry-at-uwa.edu.au 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] SEM - XL30 computer problems 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200902250534.n1P5Yp7i021319-at-ns.microscopy.com} 11, 20 -- References: {200902250534.n1P5Yp7i021319-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
The EDS cannot operate with a broken light element window and the EDS will have to be sent back for repairs to the original vendor or a detector repair company. The latter is usually less expensive. This might run from $4K - 10K. I am familiar with PulsTor / ATT and also possibly Sematech Systems might do this or know of someone. There may be others, as well. Here's some contact info:
PulseTor / AAT Jim Nicolino 1816 St. Johns Bluff Road Suite 305 Jacksonville, FL 32246 (904)646-3069 Jim Nicolino [JNicolino-at-comcast.net]
SEMTech Solutions, Inc. 6 Executive Park Drive N. Billerica, MA 01862 Mark Reynolds [mreynolds-at-semtechsolutions.com] www.sts-elionix.com Tel: (978) 663-9822 x235 Cell: (978) 828-7648 Fax: (978) 663-9823
I hope that helps.
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: analytic-at-rawbw.com [mailto:analytic-at-rawbw.com] Sent: Tuesday, February 24, 2009 7:44 PM To: dkloos-at-parallaxray.com
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Title-Subject: [Filtered] broken window on EDX detector
Question: I understand that once a 'window' on an EDX detector is broken there is no way to repair it and the crystal is destroyed. Is this true and does this apply to all types of detectors?
You would probably have to etch the resin to get PAS to work on epon sections (see recent lister communications re epoxy resin etching methods). You might also consider using methenamine silver as an alternative to get a more strongly defined end result, particularly if you are using semi-thin sections of 2 microns or less. Alternatively if you were prepared to switch resin, PAS works on any acrylic resin but strongest with methyl methacrylate as you can easily remove the resin prior to staining.
Best regards,
Alastair
Alastair McKinnon IMS Histology & EM Facility Manager, R2.21 University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk http://www.abdn.ac.uk/ims/histology/
-----Original Message----- X-from: abrun-at-hsc.unt.edu [mailto:abrun-at-hsc.unt.edu] Sent: 25 February 2009 03:37 To: Mckinnon, Alastair D.
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Email: abrun-at-hsc.unt.edu Name: Anne-Marie Brun
Organization: UNT HSC at Fort Worth Texas 76107, USA
Title-Subject: [Filtered] Epon/PAS stain
Question: Has anyone ever stained for PAS on Epon sections before? If not on Epon then what type of plastic did you use to do a PAS stain? I know it is done on paraffin embedded material, but an investigator wants the PAS staining done on Epon sections. ----? Thanks for your help Anne-Marie
{ { { { { { { { {} } } } } } } } } } } } } } Lou Ann Miller, Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine University of Illinois MC=002
Room 1204 VMBSBld 2001 S Lincoln Ave Urbana, IL 61821
217-244-1567 http://treefrog.cvm.uiuc.edu
==============================Original Headers============================== 13, 16 -- From lamiller-at-illinois.edu Wed Feb 25 06:46:26 2009 13, 16 -- Received: from expredir5.cites.uiuc.edu (expredir5.cites.uiuc.edu [128.174.5.96]) 13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PCkPCQ023780 13, 16 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 06:46:26 -0600 13, 16 -- Received: from beowulf.cvm.uiuc.edu (beowulf.cvm.uiuc.edu [130.126.16.163]) 13, 16 -- by expredir5.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id n1PCkOPv027122 13, 16 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 06:46:24 -0600 (CST) 13, 16 -- Message-Id: {8E54D6D8-1D2E-4071-BDA6-7853FBCDA1F7-at-illinois.edu} 13, 16 -- From: Lou Ann Miller {lamiller-at-illinois.edu} 13, 16 -- To: Microscopy-at-microscopy.com 13, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 13, 16 -- Content-Transfer-Encoding: 7bit 13, 16 -- Mime-Version: 1.0 (Apple Message framework v930.3) 13, 16 -- Subject: OK, one last try, PAS: url to pdf 13, 16 -- Date: Wed, 25 Feb 2009 06:46:24 -0600 13, 16 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I've always cleaned mine using diamond polishing compound. Since we are a metallographic laboratory, we have the polishing materials readily available. I use a 3-micron diamond solution with a cloth or felt pad. With the tip of my finger I swirl the aperture on the pad until the deposits are gone. This is somewhat risky, as an aperture corner can catch and fold onto itself. But this hasn't happened to me yet in the 15 years I've been doing this. I was introduced to this technique by Ken Converse of Quality Images.
Service techs are taught to hold one end of the aperture and use a Q-tip with diamond solution, rubbing in one direction only, away from where you're holding it. This polishes the aperture strip without the danger of folding.
Stu Smalinskas, P.E. Metallurgist SKF Plymouth, Michigan
--- On Tue, 2/24/09, dsherman-at-purdue.edu {dsherman-at-purdue.edu} wrote: } Hi all, } } I have a number of very pricey aperture strips that need } cleaned. The best } way would be with a plasma cleaner. Does anyone have one } who would be } willing to try to clean a strip or can you recommend } somewhere that I could } send the strips for cleaning? } } Debby } } -- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: } dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.agriculture.purdue.edu/microscopy/ }
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Email: ahmadsam-at-sabic.com Name: Dr Shahreer Ahmad
Organization: SABIC Technology Center
Title-Subject: [Filtered] Power Supply Board for Computer with XL-30 Embaded with EDAX 4i system
Question: Can anyone help me to get a power supply board Part Number 5335 171 03030 : PC Power supply, PS2, 350W. specially, if a system is not in use with someone. Manufacturer declined to supply as the system in obsolete.
Best regards.
Dr. Shahreer Ahmad, SABIC Technology Center, P.O.Box 11669, Al-Jubail 31961, Saudi Arabia Phone: +966-3-359-9175 Cell: +966-508491532 Email: ahmadsam-at-sabic.com
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Email: nama1213-at-hotmail.com Name: Dr. Naemah Al-Mansour
Organization: Kuwait University
Title-Subject: [Filtered] Cryosectioning
Question: Dear respected EMU users Does anyone know a standard technique to prepare frozen sections from fresh plant, liver and kidney samples? Usually I fix tissue in a suitable fixative depending on tissue type and objective of study (e.g. diagnostic, routine light microscopy work, TEM ...etc.) but would like to know best way for preparation of fresh frozen sections using cryostat instruments. I would be grateful if anyone can help or give suggestions on that matter stating any manual to follow for successful work in biological sample preparation. I really appreciate your help
Best regards Dr. Naemah Al-Mansour Stress Ecophysiologist Kuwait University - Faculty of Science Department of Biological Sciences Office 59 POBOX 5969 Safat 13060 State of Kuwait
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Email: lamiller-at-illinois.edu Name: Lou Ann Miller
Organization: University of Illinois
Title-Subject: [Filtered] Epoxy Section PAS
Question: PAS on EPOXY Sections
Sometimes it is desired to verify components in an epoxy section for =20 PAS positive. Because the TEM epoxy blocks have ran the tissue through =20=
many different processes than the histology tissues go through, it is =20=
necessary to run the procedure differently, and understand the =20 coloration of the final product may be different.
The first problem is the hardness of the epoxy, stains, especially at =20=
room temperature do not always stain epoxified sections well. The =20 second is that the fixative used in regular Transmission Electron =20 Microscopy contains gluteraldehyde which will cause false positive =20 staining if not delt with. The use of saturated Potassium Hydroxide =20 in Methanaol, used first off on the section to etch it, seems to take =20=
care of both of these issues.
The tissue has also gone many reactions and exposures to heavy metals =20=
as well, osmium, and uranyl acetate in the processing. These chemicals =20=
change the tissue in a way that histology tissues are not, pretty =20 bright magenta pinks are not seen. Instead a darker gray area is seen, =20=
in very thick places or sections, some of the pink may show through =20 somewhat.
It is because of this coloration, that for some instances, a counter =20 stain is not used, especially the normal 0.5% Tolluidine Blue with 1% =20=
Borate, as it will cover and obliterate the staining.
Materials needed: Hotplate beakers dispopipets kimwipes staining pad
Chemicals Used Saturated Potassium Hydroxide in Methanol 1 part KOH pellets, 2 parts MeOH, let set 1-3 days before using, will =20=
have a remaining slurry of KOH on the bottom
0.5 % Periodic Acid
Shift Reagent - Borrowed from Regular Histology Lab for their PAS
Water
Procedure
1. Cut epoxy sections, for this procedure, 0.33 to 0.5 microns =20 thick, and dry down well on a hotplate. Our thermolyne hotplate is set =20=
at 120. Dry at least one hour on the hotplate.
2. Remove slides and set the hotplate dow to 60 to cool.
3. At room temperature, with the slide level, apply the saturated KOH-=20=
MeOH mixture and allow to incubate for 20-40 minutes.
4. Rinse off gently with water, and allow to sit in water rinse baths =20=
for at least 10-15 minutes.
5. Air dry off the slide gently ( hose to air outlet with filter on =20 end) and place on the 60 set hotplate.
6. Immediately flood the slide with 0.5% Periodic Acid. Let set for =20=
40 minutes, periodically checking to be sure the slide does not =20 evaporate the solution away. Add more solution as needed.
7. Rinse off gently with water, and allow to sit in water rinse baths =20=
for at least 10-15 minutes.
8. Air dry off the slide gently ( hose to air outlet with filter on =20 end) and place on the 60 set hotplate.
9. Immediately flood the slide with the standard Shift=92s reagent from =20=
Histology. Allow to stay on the hotplate for 40-60 minutes, again =20 checking that the solution does not evaporate and adding more solution =20=
as necessary.
10. Rinse with water well, and soak in water baths for up to 15 minutes.
11. Dry slide and observe.
References:
Hayat, M.A., =93Stains and Cytochemical Methods=94, Plenum Press, New =20=
York, 1993, p 64
|--|--|--|--|--|--|--|--|----|--|--|--|--|--|--|--|--|--|--|--|--|--| Lou Ann Miller, Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine University of Illinois MC=3D002
Room 1204 VMBSBld 2001 S Lincoln Ave Urbana, IL 61821
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Email: dfish6101-at-gmail.com Name: David Fisher
Organization: Superior Technical Services
Title-Subject: [Filtered] SEM - XL30 computer problems
Question: Steve, Tell more about your computer and the video card you are talking about.
-OS? -VGA card (typically ATI Mach 64) PCI or ISA? -Overlay card (typically Coreco MX)? -Which one are you reseating?
Is the "red screen" you mention a Windows memory dump screen similar to the Blue Screen Of Death?
We have identified similar problems being caused by the power supply. Either very noisy AC going into the computer, or a weak PC supply, specifically the 3.3V.
More details will be helpful, as we may have surplus spares. Although the video boards are rare and valuable you should be able to solve your problem for significantly less than $15k AU.
Dave David Fisher Superior Technical Services 16307 NE Cameron Blvd. Portland, OR 97230 (503) 764-1456 x3
Dear Dr. Al-Mansour- For a number of years, we have used a variation on the protocol outlined in: Barthel & Raymond (1990) J. Histochem. Cytochem 38(9) 1283-1388 the authors were working with eyes, which are expecially difficult due.
Briefly, after fixation with pfa (2% or 4% depending on your requirements) we cryoprotect in a 30% sucrose in PBS, then encapsulate and freeze in a 1:2 mixture of the 30% sucrose and OCT. This yields smooth-cutting blocks and remarkable good structure. My facility is in a medical college, so we deal exclusively with (mammalian) tissue samples. I can't tell you anything about working with plant material.
Lee
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-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Margo, I believe that the "common knowledge" is not always correct. My understanding is that the SiLi crystal is usually destroyed by having the bias applied when warm. One of the ways to avoid that is to always turn the bias off before unplugging the detector. The bias supply will drain the voltage off, whereas unplugging the bias will leave a small, but very efficient, capacitor charged to the bias voltage for, perhaps, longer than it takes the detector to warm up.
The broken window may actually be your only problem, although still expensive to repair, in part because the window is expensive and the dewar must be repumped and leak-checked.
My customers have had good experiences with Jim Nicholino. No financial interest, but I like my customers to be happy.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: analytic-at-rawbw.com [mailto:analytic-at-rawbw.com] Sent: Tuesday, February 24, 2009 10:33 PM To: kenconverse-at-qualityimages.biz
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Title-Subject: [Filtered] broken window on EDX detector
Question: I understand that once a 'window' on an EDX detector is broken there is no way to repair it and the crystal is destroyed. Is this true and does this apply to all types of detectors?
If a window on a detector blows, it depends upon how it blew will determine if the crystal is damaged or not.
If the detector never sees atmosphere and is undamaged, it can run with the broken window without a problem.
Another source for fixing EDS detectors is Garry Baerwalt of Max Detectors in Middletown Wisconsin. I don't remember the address but his email address is garry-at-maxdetector.com
Good luck,
dj
On Tue, 24 Feb 2009, analytic-at-rawbw.com wrote:
} Email: analytic-at-rawbw.com } Name: Margo Gill-Linscott } } Organization: Analyticus, Inc. } } Title-Subject: [Filtered] broken window on EDX detector } } Question: I understand that once a 'window' on an EDX detector is } broken there is no way to repair it and the crystal is destroyed. Is } this true and does this apply to all types of detectors? } } Login Host: 198.144.223.152 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:58 2009 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1P3Rt9m008169 } 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb 2009 21:27:58 -0600 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240801c5ca6b297dc1-at-[206.69.208.22]} } 6, 11 -- Date: Tue, 24 Feb 2009 21:27:55 -0600 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: analytic-at-rawbw.com (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: broken window on EDX detector } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 19 -- From dljones-at-bestweb.net Wed Feb 25 09:07:05 2009 9, 19 -- Received: from smtp1.bestweb.net (smtp1.bestweb.net [209.94.103.41]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PF75Jh008510 9, 19 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 09:07:05 -0600 9, 19 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202]) 9, 19 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 19 -- (No client certificate requested) 9, 19 -- by smtp1.bestweb.net (Postfix) with ESMTPS id 72735125434; 9, 19 -- Wed, 25 Feb 2009 10:07:04 -0500 (EST) 9, 19 -- Date: Wed, 25 Feb 2009 14:39:34 +0000 (UTC) 9, 19 -- From: dljones {dljones-at-bestweb.net} 9, 19 -- To: analytic-at-rawbw.com 9, 19 -- cc: microscopy-at-microscopy.com 9, 19 -- Subject: Re: [Microscopy] viaWWW: broken window on EDX detector 9, 19 -- In-Reply-To: {200902250335.n1P3Z9ol018420-at-ns.microscopy.com} 9, 19 -- Message-ID: {Pine.BSF.4.64.0902251422380.94009-at-monet.bestweb.net} 9, 19 -- References: {200902250335.n1P3Z9ol018420-at-ns.microscopy.com} 9, 19 -- MIME-Version: 1.0 9, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Jesse, Thank you for the clarification. And please relay my thanks to Sheri Kurland, Mark Huller and Steve Gazda for their excellent service and the amazing speed at which they found and sent us a manual for our CM200. Sincerly, Valerie
} Val, } } Just to clarify, it was EDAX that destroyed the last of their manuals } for your detector. We still have copies of ours. I'm working on } finding them for you though. Most are in use out at customers' sites or } for internal reference. } } Jesse Doerr } } Field Service Engineer } FEI Company } (503)267-9620 } jesse.doerr-at-fei.com } } -----Original Message----- } From: vlynch-at-mail.wsu.edu [mailto:vlynch-at-mail.wsu.edu] } Sent: Thursday, February 05, 2009 1:25 PM } To: Doerr, Jesse } Subject: [Microscopy] RE: Philips TEM CM-200 Manual? } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America
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I may have to stand correctable here but my experience says that the bias will automatically shut off if the FET and detector crystal are not cold enough. When the window breaks, that ought to cause the -750V to shut off.
The detector is a reverse biased drifted Silicon diode and the reverse bias creates a big depletion region. The output from the detector crystal is fed to a FET amplifier transistor which is also cooled to reduce noise. Its signal is then sent up stream to room temperature electronics and on to the pulse processor in the PC.
Since the detector probe is under vacuum, venting the SEM chamber too fast or pulling open the door before full venting will likely crack the EDS window. AFIK, all makers use Moxtec windows, mostly the .3u thick SUTW. The detectors with no windows are a separate subject.
gary g.
At 06:49 AM 2/25/2009, you wrote:
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I would suppose the new detectors might have a circuit to shut off HV, but I can certainly say that many old detectors did not. We have such a one still in service. We lost one crystal because it warmed up under improper conditions.
I suppose it would be the pressure pulse that would rupture the window. We have not had one fail due to anyone slamming the SEM chamber closed or pulling it open - yet. I don't care to run the experiment on my nickel. Maybe the guys at Moxtek have run those experiments as part of their R&D.
Warren Straszheim
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Wednesday, February 25, 2009 11:27 AM To: wesaia-at-iastate.edu
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Particles represent a microanalytical challenge. Whether your particles are desired or undesired, natural or man-made, nano-, micro- or milli-scaled, no one technique is optimal for determining all the important physical properties. A knowledgable analyst must understand the strengths and weaknesses of each available technique. This conference is designed to provide just such an understanding for microbeam techniques including EDS, WDS, AEM, SIMS, XRD, synchrotron XRF and numerical simulation. The target audience includes scientists in industry, government, academia and commercial laboratories. The speakers will provide enough background for a motivated novice but will aim the majority of the content at practitioners who wish to learn new techniques and to pick up advanced skills.
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Suppose someone wants to mothball a LN2 chilled detector for a period of time.
Is this feasible without significant damage?
What precautions should be taken (bias off and voltage drained) prior to warming up, etc?
What are the negative aspects of doing this (loss of resolution, presumably)?
Thanks for the information. -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Roy Beavers asked about measuring the height profile of a polyimide laser-treated surface with nanometer resolution. He wrote: "SEM images have been very difficult to achieve due to charging and small feature size. Thinking a surface profiler or AFM may give better results."
We have been providing commercial AFM analysis services for 19 years. We have unique capabilities, which include the ability to measure small heights with fine resolution (to 0.2 nm, i.e. single atomic steps) and to measure extremely tall objects (to 24 um) with resolution about 1 nm.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: rbeavers-at-mail.smu.edu To: donc-at-asmicro.com Sent: Wednesday, February 18, 2009 1:54 PM Subject: [a] [Microscopy] Laser Surface Profiler
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Group,
Trying to locate a lab in the North Texas area that has Laser 3D Surface profile capabilities. Need to profile surface of a laser treated polyimide film on glass substrate to nanometer resolution. SEM images have been very difficult to achieve due to charging and small feature size. Thinking a surface profiler or AFM may give better results.
Prefer University labs but will be glad to hear from commercial labs as well.
Thanks
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
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} Suppose someone wants to mothball a LN2 chilled detector for a period of time. } Is this feasible without significant damage? } What precautions should be taken (bias off and voltage drained) prior } to warming up, etc? } ?What are the negative aspects of doing this (loss of resolution, presumably)?
Hi John
I did this over a 1-month vacation period once, having been assured by the manufacturer that it was perfectly OK.
The vacuum and resolution both deteriorated to the point of being unusable for quantitative work.
After this had happened, and on further rather annoyed questioning, the manufacturer said that some deterioration in performance was to be expected.
I chose to replace it, with one from a different maker!
I will never, never do this again unless it is 100% unavoidable.
cheers
Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 15, 30 -- From r.sims-at-auckland.ac.nz Wed Feb 25 13:49:26 2009 15, 30 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34]) 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PJnP3r020068 15, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Feb 2009 13:49:26 -0600 15, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id A4D551A2C9; 15, 30 -- Thu, 26 Feb 2009 08:49:24 +1300 (NZDT) 15, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 15, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 15, 30 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 30 -- with ESMTP id R01SrwJsNjjA; Thu, 26 Feb 2009 08:49:24 +1300 (NZDT) 15, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 15, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) 15, 30 -- (No client certificate requested) 15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 7ED311A284; 15, 30 -- Thu, 26 Feb 2009 08:49:23 +1300 (NZDT) 15, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 30 -- To: bozzola-at-siu.edu 15, 30 -- Date: Thu, 26 Feb 2009 08:51:29 +1300 15, 30 -- MIME-Version: 1.0 15, 30 -- Subject: Re: [Microscopy] Another EDX detector question 15, 30 -- CC: Microscopy-at-Microscopy.Com 15, 30 -- Message-ID: {49A65811.27551.45F397-at-r.sims.auckland.ac.nz} 15, 30 -- Priority: normal 15, 30 -- In-reply-to: {200902251920.n1PJKcS4025632-at-ns.microscopy.com} 15, 30 -- References: {200902251920.n1PJKcS4025632-at-ns.microscopy.com} 15, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 30 -- Content-type: text/plain; charset=US-ASCII 15, 30 -- Content-transfer-encoding: 7BIT 15, 30 -- Content-description: Mail message body ==============================End of - Headers==============================
Hi John, Hopefully others with more expertise in this area will chime in because my information is old.
My understanding is that when Kevex brought out their windowless detector (too many years ago), what they found was that warming, in and of itself, was not a problem as long as the bias was gone. The big problem was condensable contaminants that would be captured by the zeolites in the dewar, released upon warming and then contaminating the SiLi crystal. They were perfectly willing to ship those detectors warm, but the appendage pump had a battery operated controller that shipped with it. Basically they were far more concerned about the quality if the vacuum than the temperature of the crystal.
We've all heard lots of stories about how one person's detector warmed up once and was trashed, while others repeatedly warm their detectors and keep right on truckin'. What I take from Kevex's experience is that it's kind of a crap shoot and depends upon what is in the vacuum part of the dewar, whether from manufacturing residues or from leaking and what condensables might have entered through the leak(s).
The other slight risk with warming is that if you've had a bad leak for a while, when the zeolites warm up, all the gasses they've captured are going to be released, possibly going to considerable positive pressure and blowing the window. It's probably not a concern unless your lN2 consumption is very high, indicating a poor vacuum in the dewar, but thin windows make it more of a concern than Be windows.
Is it PGT that made the LEAP detector? The dewar looks nothing like your standard dewar, doesn't hold much lN2, and functions for years going warm and cold, warm and cold. It may be that they got rid of the zeolites (hence the sorption pumping) to eliminate the possibility of contaminating the crystal upon warming. Maybe someone who has some info can comment on that.
I think the answer to your question is, "It's a crap-shoot." May the force be with you.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Wednesday, February 25, 2009 2:22 PM To: kenconverse-at-qualityimages.biz
This thread brought up a question.
Suppose someone wants to mothball a LN2 chilled detector for a period of time.
Is this feasible without significant damage?
What precautions should be taken (bias off and voltage drained) prior to warming up, etc?
What are the negative aspects of doing this (loss of resolution, presumably)?
Thanks for the information. -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Yes, that was what I concluded, the vacuum is everything, and in a windowed detector, warming up can allow gases to be desorbed from the zeolites (or whatever "getter" is used).
If the vacuum deteriorates, the internals run a bit warmer because the thermal insulation is compromised.
JEOL had for a while an integrated EDS detector which could be warmed and recooled repeatedly because it used the SEM vacuum to revacuate the detector, as I understood it. Seemed like a pretty good idea to me but I don't know if it was good in practice or if they still offer it. Is that what that LEAP detector was?
cheers Ritchie
On 25 Feb 2009 at 14:00, kenconverse-at-qualityimages.biz wrote:
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Hi John, Hopefully others with more expertise in this area will chime in because my information is old.
My understanding is that when Kevex brought out their windowless detector (too many years ago), what they found was that warming, in and of itself, was not a problem as long as the bias was gone. The big problem was condensable contaminants that would be captured by the zeolites in the dewar, released upon warming and then contaminating the SiLi crystal. They were perfectly willing to ship those detectors warm, but the appendage pump had a battery operated controller that shipped with it. Basically they were far more concerned about the quality if the vacuum than the temperature of the crystal.
We've all heard lots of stories about how one person's detector warmed up once and was trashed, while others repeatedly warm their detectors and keep right on truckin'. What I take from Kevex's experience is that it's kind of a crap shoot and depends upon what is in the vacuum part of the dewar, whether from manufacturing residues or from leaking and what condensables might have entered through the leak(s).
The other slight risk with warming is that if you've had a bad leak for a while, when the zeolites warm up, all the gasses they've captured are going to be released, possibly going to considerable positive pressure and blowing the window. It's probably not a concern unless your lN2 consumption is very high, indicating a poor vacuum in the dewar, but thin windows make it more of a concern than Be windows.
Is it PGT that made the LEAP detector? The dewar looks nothing like your standard dewar, doesn't hold much lN2, and functions for years going warm and cold, warm and cold. It may be that they got rid of the zeolites (hence the sorption pumping) to eliminate the possibility of contaminating the crystal upon warming. Maybe someone who has some info can comment on that.
I think the answer to your question is, "It's a crap-shoot." May the force be with you.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Wednesday, February 25, 2009 2:22 PM To: kenconverse-at-qualityimages.biz
This thread brought up a question.
Suppose someone wants to mothball a LN2 chilled detector for a period of time.
Is this feasible without significant damage?
What precautions should be taken (bias off and voltage drained) prior to warming up, etc?
What are the negative aspects of doing this (loss of resolution, presumably)?
Thanks for the information. -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I have three EDS systems in my lab, and several times I did let them go to room temperature, each time unplugging them completely. No damage at all.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Phone: (816) 235-2072 Fax: (816) 235-5524 Web: http://www.umkc.edu/dentistry/microscopy } } } } Suppose someone wants to mothball a LN2 chilled detector for } a period of time. } } Is this feasible without significant damage? } } What precautions should be taken (bias off and voltage } drained) prior } } to warming up, etc? } } ?What are the negative aspects of doing this (loss of } resolution, presumably)? } } Hi John } } I did this over a 1-month vacation period once, having been assured by
} the manufacturer that it was perfectly OK. } } The vacuum and resolution both deteriorated to the point of being } unusable for quantitative work. } } After this had happened, and on further rather annoyed questioning, } the manufacturer said that some deterioration in performance was to be
} expected. } } I chose to replace it, with one from a different maker! } } I will never, never do this again unless it is 100% unavoidable. } } cheers } } Ritchie } } } } } -- } Ritchie Sims Ph D Phone : 64 9 } 3737599 ext 87713 } Microanalyst Fax : } 64 9 3737435 } Department of Geology email : } r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original } Headers============================== } 15, 30 -- From r.sims-at-auckland.ac.nz Wed Feb 25 13:49:26 2009 15, 30 } -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz } [130.216.12.34]) } 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n1PJnP3r020068 } 15, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Feb } 2009 13:49:26 -0600 } 15, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP } id A4D551A2C9; } 15, 30 -- Thu, 26 Feb 2009 08:49:24 +1300 (NZDT) } 15, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } 15, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) } 15, 30 -- by localhost (larry.its.auckland.ac.nz } [127.0.0.1]) (amavisd-new, port 10024) } 15, 30 -- with ESMTP id R01SrwJsNjjA; Thu, 26 Feb 2009 } 08:49:24 +1300 (NZDT) } 15, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz } [130.216.59.18]) } 15, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) } 15, 30 -- (No client certificate requested) } 15, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP } id 7ED311A284; } 15, 30 -- Thu, 26 Feb 2009 08:49:23 +1300 (NZDT) } 15, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 30 } -- To: bozzola-at-siu.edu 15, 30 -- Date: Thu, 26 Feb 2009 } 08:51:29 +1300 15, 30 -- MIME-Version: 1.0 15, 30 -- Subject: } Re: [Microscopy] Another EDX detector question 15, 30 -- CC: } Microscopy-at-Microscopy.Com 15, 30 -- Message-ID: } {49A65811.27551.45F397-at-r.sims.auckland.ac.nz} } 15, 30 -- Priority: normal } 15, 30 -- In-reply-to: {200902251920.n1PJKcS4025632-at-ns.microscopy.com} } 15, 30 -- References: {200902251920.n1PJKcS4025632-at-ns.microscopy.com} } 15, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 30 -- } Content-type: text/plain; charset=US-ASCII 15, 30 -- } Content-transfer-encoding: 7BIT 15, 30 -- } Content-description: Mail message body } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 23 -- From DusevichV-at-umkc.edu Wed Feb 25 16:55:24 2009 7, 23 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1PMtNDc002882 7, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 16:55:24 -0600 7, 23 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Wed, 25 Feb 2009 16:55:23 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="US-ASCII" 7, 23 -- Subject: Re: Another EDX detector question 7, 23 -- Date: Wed, 25 Feb 2009 16:55:22 -0600 7, 23 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB7E0-at-KC-MSX1.kc.umkc.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Re: Another EDX detector question 7, 23 -- thread-index: AcmXgkvTxziBaRRYTMuSR9KTxkwD0wAFF3NwAAFZkWA= 7, 23 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 23 -- To: {Microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 25 Feb 2009 22:55:23.0233 (UTC) FILETIME=[240D8D10:01C9979C] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1PMtNDc002882 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cannonmp-at-comcast.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cannonmp-at-comcast.net Name: Bart Cannon
Organization: Cannon Microprobe
Title-Subject: [Filtered] EDS Detectors and Dewars
Question: I thought that my experiences with SiLi x-ray detectors and dewars might be of interest.
I have a 1980 Kevex EDS detector. It had been at room temp for six years when I bought it in 1990. I dismantled the cryo-tip and extended it and its vacuum enclosure by nearly a foot in order to accomodate an extreme geometry on my ARL SEMQ electron probe.
At the same time, I constructed some valving and tubulation in order to make the dewar vacuum continuous with my system vacuum when a valve was opened.
Since 1990 I have routinely let my dewar go dry several times a week. I only add enough LN2 to complete a day's work. When the dewar goes dry, I pump it down.
Last year there was a five day period during which my dewar went dry and I had left the bias on (-640 volts). I felt the end had come, but I pumped down the system and added LN2. Everything was fine and my 29 year old detector was back to better than 160 ev resolution at Mn ka.
I have a friend with detector experience. His comment was that I have one of the good ones.
I second Vladimir's experience. I had used an EDAX Sapphire Dewar 204 detector and let it sit at room temperature for weeks. No problem. SafeFill was turned on when the detector was needed--it loads the LN2. Again, no problem.
Nowadays, the EDAX Apollo 40 SDD makes a huge difference. I figure that all or most SDD maker's SDD systems will be vastly superior to legacy Si(Li) EDS detectors.
There are argumentative issues with SDD specs but the fact remains, IMO, that SDD will kill Si(Li) and LN2 systems over time. The current generation of SDD is too phenomenal to dismiss. Money is always an issue. ROI and up-time are also factors. The SDD in and of itself was evolutionary and now it appears to me to be revolutionary with what I think is the third generation of SDD detector chips. The differences from Si(Li) are stunning!
As a side note, the EDAX Apollo 40 has a small ion pump to maintain vacuum. This is very nice. I had suggested this some time ago for the Si(Li) flavors of detectors. I have no idea if they did this based on my suggestion. But they did do it. Thus, the getters issue is gone. This was an issue with non-LN2 detectors...old history.
DISCLAIMER: I have no financial interest in EDAX/Ametek/TSL other than being a very satisfied customer of their products and service.
gary g.
At 02:57 PM 2/25/2009, you wrote:
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==============================Original Headers============================== 14, 20 -- From gary-at-gaugler.com Wed Feb 25 19:53:44 2009 14, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1Q1rh8b002917 14, 20 -- for {microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 19:53:43 -0600 14, 20 -- Message-Id: {200902260153.n1Q1rh8b002917-at-ns.microscopy.com} 14, 20 -- Received: (qmail 26504 invoked from network); 25 Feb 2009 17:52:04 -0800 14, 20 -- Received: by simscan 1.1.0 ppid: 26500, pid: 26502, t: 0.1716s 14, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 14, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 14, 20 -- by smtp2 with SMTP; 25 Feb 2009 17:52:04 -0800 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 20 -- Date: Wed, 25 Feb 2009 17:53:38 -0800 14, 20 -- To: DusevichV-at-umkc.edu 14, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 14, 20 -- Subject: Re: [Microscopy] Re: Another EDX detector question 14, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 14, 20 -- In-Reply-To: {200902252257.n1PMvE4j005317-at-ns.microscopy.com} 14, 20 -- References: {200902252257.n1PMvE4j005317-at-ns.microscopy.com} 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I have a question concerning immunogold labelling of membrane proteins on tissue embedded in LR White: We labelled a positive control - a cytoplasmatic protein - on muscle tissue embedded in LR White and tested two antibodies against membrane proteins.
The positive control worked extremely well, but the two membrane proteins do not show such a clear labelling- it is useful but there is a lot of background and unspecific labelling in the nucleoplasm.
Is it possible that the membranes are partially washed out or effected by the dehydration process?
Which procedure do you recommend (pre-embedding? or post- embedding with special dehydration steps?) for labelling membrane proteins?
thank you
Gerd
-- Dr. Gerd Leitinger
Laboratory Coordinator Core Facility Ultrastructure Analysis Center for Medical Research (ZMF) Medical University of Graz
Postal address: Institute of Cell Biology, Histology and Embryology Harrachgasse 21 8010 Graz Austria Tel. +43 316 380 4237 Fax. +43 316 380 9625 Mailto: Gerd.Leitinger-at-medunigraz.at
==============================Original Headers============================== 12, 22 -- From gerd.leitinger-at-medunigraz.at Thu Feb 26 02:14:12 2009 12, 22 -- Received: from si069.medunigraz.at (si082.meduni-graz.at [193.170.105.82]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q8ECKj028517 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 02:14:12 -0600 12, 22 -- X-AuditID: 0ac80c45-aa347bb000000f5d-b2-49a64f545bca 12, 22 -- Received: from si062.medunigraz.at (si062.meduni-graz.at [10.200.12.62]) 12, 22 -- by si069.medunigraz.at (Symantec Mail Security) with ESMTP id 3ADDF4DC002 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 09:14:12 +0100 (CET) 12, 22 -- Received: from connect_62-MTA by si062.medunigraz.at 12, 22 -- with Novell_GroupWise; Thu, 26 Feb 2009 09:14:12 +0100 12, 22 -- Message-Id: {49A65D59.E434.00B0.0-at-medunigraz.at} 12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 12, 22 -- Date: Thu, 26 Feb 2009 09:13:53 +0100 12, 22 -- From: "Gerd Leitinger" {gerd.leitinger-at-medunigraz.at} 12, 22 -- To: {Microscopy-at-microscopy.com} 12, 22 -- Subject: TEM: Membrane proteins in post-embedding immunogold 12, 22 -- Mime-Version: 1.0 12, 22 -- Content-Type: text/plain; charset=US-ASCII 12, 22 -- Content-Disposition: inline 12, 22 -- X-Brightmail-Tracker: AAAAAA== 12, 22 -- Content-Transfer-Encoding: 8bit 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1Q8ECKj028517 ==============================End of - Headers==============================
It has been reported that membrane proteins can be extracted from membranes, mechanically, although I can not recall any proteins having been washed out. Certainly they may be affected by fixation and dehydration. Which approach? There are many approaches that can get good results in immunolabeling, but I think first you might want to look at your results as they are and try to understand where signal and background originate from. Can you describe the species of primary antibodies and what kind of gold conjugate (protein A.. secondary antibody) you used in both cases? What are the negative controls like, i.e. the ones without primary? What source (species) was the tissue from?
Jan Leunissen
Aurion http://www.aurion.nl
On 26/02/2009, at 9:14 PM, gerd.leitinger-at-medunigraz.at wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all: } } I have a question concerning immunogold labelling of membrane } proteins on tissue embedded in LR White: } We labelled a positive control - a cytoplasmatic protein - on muscle } tissue embedded in LR White and tested two antibodies against } membrane proteins. } } The positive control worked extremely well, but the two membrane } proteins do not show such a clear labelling- it is useful but there } is a lot of background and unspecific labelling in the nucleoplasm. } } Is it possible that the membranes are partially washed out or } effected by the dehydration process? } } Which procedure do you recommend (pre-embedding? or post- embedding } with special dehydration steps?) for labelling membrane proteins? } } thank you } } Gerd } } } -- } Dr. Gerd Leitinger } } Laboratory Coordinator } Core Facility Ultrastructure Analysis } Center for Medical Research (ZMF) } Medical University of Graz } } Postal address: } Institute of Cell Biology, Histology and Embryology } Harrachgasse 21 } 8010 Graz } Austria } Tel. +43 316 380 4237 } Fax. +43 316 380 9625 } Mailto: Gerd.Leitinger-at-medunigraz.at } } } } } ==============================Original } Headers============================== } 12, 22 -- From gerd.leitinger-at-medunigraz.at Thu Feb 26 02:14:12 2009 } 12, 22 -- Received: from si069.medunigraz.at (si082.meduni-graz.at } [193.170.105.82]) } 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n1Q8ECKj028517 } 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 } 02:14:12 -0600 } 12, 22 -- X-AuditID: 0ac80c45-aa347bb000000f5d-b2-49a64f545bca } 12, 22 -- Received: from si062.medunigraz.at (si062.meduni-graz.at } [10.200.12.62]) } 12, 22 -- by si069.medunigraz.at (Symantec Mail Security) with } ESMTP id 3ADDF4DC002 } 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 } 09:14:12 +0100 (CET) } 12, 22 -- Received: from connect_62-MTA by si062.medunigraz.at } 12, 22 -- with Novell_GroupWise; Thu, 26 Feb 2009 09:14:12 +0100 } 12, 22 -- Message-Id: {49A65D59.E434.00B0.0-at-medunigraz.at} } 12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 } 12, 22 -- Date: Thu, 26 Feb 2009 09:13:53 +0100 } 12, 22 -- From: "Gerd Leitinger" {gerd.leitinger-at-medunigraz.at} } 12, 22 -- To: {Microscopy-at-microscopy.com} } 12, 22 -- Subject: TEM: Membrane proteins in post-embedding immunogold } 12, 22 -- Mime-Version: 1.0 } 12, 22 -- Content-Type: text/plain; charset=US-ASCII } 12, 22 -- Content-Disposition: inline } 12, 22 -- X-Brightmail-Tracker: AAAAAA== } 12, 22 -- Content-Transfer-Encoding: 8bit } 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n1Q8ECKj028517 } ==============================End of - } Headers==============================
==============================Original Headers============================== 11, 19 -- From leunissen-at-aurion.nl Thu Feb 26 02:38:59 2009 11, 19 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q8cv7S010253 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 02:38:58 -0600 11, 19 -- Received: from [192.168.1.50] (really [122.57.247.59]) by fep01.xtra.co.nz 11, 19 -- with ESMTP 11, 19 -- id {20090226083851.HNVO7189.fep01.xtra.co.nz-at-[192.168.1.50]} 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 21:38:51 +1300 11, 19 -- Message-Id: {6546CB94-4E4D-4AFF-AED2-3FEACEBC939D-at-aurion.nl} 11, 19 -- From: Jan Leunissen {leunissen-at-aurion.nl} 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- In-Reply-To: {200902260814.n1Q8EVGe028864-at-ns.microscopy.com} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 19 -- Subject: Re: [Microscopy] TEM: Membrane proteins in post-embedding immunogold 11, 19 -- Date: Thu, 26 Feb 2009 21:38:50 +1300 11, 19 -- References: {200902260814.n1Q8EVGe028864-at-ns.microscopy.com} 11, 19 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Apologies: Due to some problems in connecting with the MSA-Listserver-forum duringe the last 2 days this my message comes a little bit late...
Good morning,
Dear Anne-Marie Brun, your quest (at least for me) is likely a very special one.
It might be wise to tell your "investigator" that he is asking for your doing "extravagant work" to please him.
Needless to remind that PAS staining (I mean "real PAS- = } periodic acid -Schiff-reaction- { Staining" like done in histochemistry on paraffin or cryosections) IS NOT POSSIBLE to facilitate ON regular EPON / EPOXY SECTIONS (with some exceptions, provided that initially blocking of (free) aldehydes after fixation has been done).
This on the one hand is due to the primary/secondary fixation usually used in electron microscopic specimen preparations (i.e. FA [formaldehyde]-GA, GA=[glutaraldehyde] fixation, OsO4-postfix) as well as on the other hand due to the unfavorable properties of the resin used: hydrophobic [and firmly polymerized] epoxid/Epon.
In routine TEM-processing, after the primary fixation as done usually, nobody (perhaps only somebody) will block free aldehydes (which are left from FA-GA-fixation) in the tissue after fixation (e.g. by Na-(sodium-)borohydride-solution, or PBS containing 50 mM glycine , or dimedone, or IMO - better/best - 0.05M NH4Cl = ammoniumchloride in 0.1 M washing buffer 10-25 min at Room-Temp) on a routine basis (some -many?- do it when immunolabelling IEM is the final task).
Without using "Aldehyde-blocking" solutions you will get "false-positive" results of PAS staining.
Exceptions for realizing "PAS"-staining on 100% EPON-/solely epoxy-resin sections (perhaps/certainly my opinion(s), not exhaustive) are:
- Etching of resin prior to staining (e.g. sodium(Na)or potassium (K)-ethylate-solution, and other special "ripened" solvents) certainly is necessary / a prerequisite for.
There are a lot of (old and older) papers/articles on that [mostly 1970ies/1980ies] which not only were published because of demands in finding "real" classical histochemical stainings on (epoxy) resin sections but also due to the advent of "immuno...." applications in (T)EM...this was before the advent of } hydro {philic acrylic resins like LR WHITE, LR GOLD, UNICRYL, and the LOWICRYLS.
Some (many old) publications deal with the routine use of resin combinations like epon - araldite...which supposedly (and in hands of "specialists", who certainly will catch the speaker's eye in this MSA thread most sucessfully) provide almost true "histochemical" stainings (also after "etching" sections or block faces).
- Use of methacrylate resin embedded tissue: for this task I can provide you with a recipe out from the famous (but exhausted/out of print) booklet of BURNS and BRETSCHNEIDER (1981) - Thin is in: Plastic Embedding of Tissue for Light Microscopy....
- and, last but not least you might fail also due to using not the correct staining solutions.... (hydrophobic as well as hydrophilic resins at large perhaps need other formulae or application steps than formerly paraffin-embedded or cryo-preserved material sections handled for LM-histochemistry.
In 2006 there was some traffic on the MSA-Listserver (and I am confident that in the ARCHIVES a lot of requests deal with the question "PAS-Staining of resin sections") and ONE good reply was (by Dieter D. Bosshardt, Switzerland):
{ { Some years ago, I did PAS staining of Epon sections (0.5-1.0 micron thick). There is a good reference explaining the procedure: Schroeder HE, Rossinsky K, Müller W (1980) An established routine method for differential staining of epoxy-embedded tissue sections. } {Microscopica Acta} 83,111-116. Hope this helps. Best regards, Dieter -- Dieter D. Bosshardt, Ph.D. University of Bern School of Dental Medicine Department of Periodontology & Fixed Prosthodontics Post Box 64 Freiburgstrasse 7 CH-3010 Bern 10 Switzerland http://dent.unibe.ch Phone: +41-31-632 86 05 Fax: +41-31-632 49 31 mailto:dieter.bosshardt-at-zmk.unibe.ch
For now, I stop here and shall see what other listers will offer...and meanwhile I shall try to get a reprint of the article mentioned above....
Best wishes and regards,
Wolfgang Muss
OR Dr. phil. Wolfgang Muss Head of EM-Lab Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively (same Lab, same address)
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
Ankuendigung namens der (Information on behalf of) Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org {
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} -----Ursprüngliche Nachricht----- } Von: abrun-at-hsc.unt.edu [mailto:abrun-at-hsc.unt.edu] } Gesendet: Mittwoch, 25. Februar 2009 04:36 } An: Muß Wolfgang } Betreff: [Microscopy] Epon/PAS stain ...?? } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --------------------------------------------------------------------------- } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber,so when replying } please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } Email: abrun-at-hsc.unt.edu } Name: Anne-Marie Brun } Organization: UNT HSC at Fort Worth Texas 76107, USA } Title-Subject: } Epon/PAS stain } Question: } } Has anyone ever stained for PAS on Epon sections before? } } If not on Epon then what type of plastic did you use to do a } PAS stain? } I know it is done on paraffin embedded material, but an } investigator wants the PAS staining done on Epon sections. } } ----? } Thanks for your help } Anne-Marie } } Login Host: 129.120.96.228 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Tue Feb 24 21:27:32 2009 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n1P3RVv3007908 } 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 24 Feb } 2009 21:27:31 -0600 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240800c5ca6b1378b7-at-[206.69.208.22]} } 6, 11 -- Date: Tue, 24 Feb 2009 21:27:29 -0600 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: abrun-at-hsc.unt.edu (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Epon/PAS stain } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of Headers============================== }
==============================Original Headers============================== 35, 35 -- From W.Muss-at-salk.at Thu Feb 26 03:58:51 2009 35, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 35, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1Q9wooi026478 35, 35 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 03:58:50 -0600 35, 35 -- Received: from localhost (localhost [127.0.0.1]) 35, 35 -- by hermes.salk.at (Postfix) with ESMTP id F194FC386E; 35, 35 -- Thu, 26 Feb 2009 10:58:48 +0100 (CET) 35, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 35, 35 -- Received: from hermes.salk.at ([127.0.0.1]) 35, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 35, 35 -- with ESMTP id kD7SJvbS6wAL; Thu, 26 Feb 2009 10:58:48 +0100 (CET) 35, 35 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 35, 35 -- by hermes.salk.at (Postfix) with ESMTP id 7E876C383D; 35, 35 -- Thu, 26 Feb 2009 10:58:48 +0100 (CET) 35, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 35, 35 -- Thu, 26 Feb 2009 10:58:48 +0100 35, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 35, 35 -- Content-class: urn:content-classes:message 35, 35 -- MIME-Version: 1.0 35, 35 -- Content-Type: text/plain; 35, 35 -- charset="iso-8859-1" 35, 35 -- Subject: [Microscopy] Re: Epon/PAS stain ...?? (Sorry for longness) 35, 35 -- Date: Thu, 26 Feb 2009 10:58:48 +0100 35, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0881-at-N1RZ116.lksdom21.lks.local} 35, 35 -- X-MS-Has-Attach: 35, 35 -- X-MS-TNEF-Correlator: 35, 35 -- Thread-Topic: [Microscopy] Re: Epon/PAS stain ...?? (Sorry for longness) 35, 35 -- Thread-Index: AcmX+NG+gYN9Cap5S3uzXxIql6UFOg== 35, 35 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 35, 35 -- To: {microscopy-at-microscopy.com} , {wij.muss-at-aon.at} , 35, 35 -- =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 35, 35 -- X-OriginalArrivalTime: 26 Feb 2009 09:58:48.0642 (UTC) FILETIME=[D1ECF220:01C997F8] 35, 35 -- X-Scanned-By: SALK-Content-Filter 35, 35 -- Content-Transfer-Encoding: 8bit 35, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1Q9wooi026478 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Attaching a camera to a Nikon SMZ-2T phototube
Question: Does anyone have an older SMZ-2T stereo microscope with a coolpix camera setup on the phototube? The camera on ours was stolen a few years ago, and I'm trying to figure out what I need to restore picture taking abilities. I minimally want to achieve lab-notebook quality pictures of protein crystals. This is for a new class I'm teaching and of course there's no real budget for purchasing a whole new setup, so I'm trying to find replacement parts at minimal cost.
What remains on the microscope is the phototube itself, which I think has a C-mount male thread on the top. This is a metal beige piece that I think is the V-T phototube, and it is removable.
Inside the phototube is a removable TV lens (1X/16).
The old camera was a Nikon Coolpix 990 with a 28mm thread. It seems pretty straightforward to purchase a used camera on Ebay, but from extensively consulting Mr. Google, I think I'm also missing a relay lens that is supposed to go between the camera and the phototube. The lens I think I might be missing is the MDC-A relay lens, which is no longer being made by Nikon
Can anyone confirm this thinking? I'm not exactly sure that the phototube is a C-mount, but I do know that it has male threads I can't screw 28mm threads directly onto it. The threads on the potential c-mount seem more widely spread than the threads on a female 28mm camera filter.
If this is a C-mount does anyone know if the "Nikon camera lens adaptor for C-mount microscope" item on ebay would be an appropriate MDC-A substitute. http://cgi.ebay.com/NIKON-CAMERA-LENS-ADAPTER-FOR-C-MOUNT-MICROSCOPE_W0QQitemZ250378986591QQcmdZViewItemQQptZLH_DefaultDomain_0?hash=item250378986591&_trksid=p3286.c0.m14&_trkparms=72%3A1205|66%3A2|65%3A12|39%3A1|240%3A1318|301%3A1|293%3A1|294%3A50
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Title-Subject: [Filtered] Images of plant cell walls & protein bodies
Question: I am teaching a class on food proteins to Culinary school undergrads and want to show some SEM images of protein bodies in plant cells. Do you have any suggetsions for a good website to get some clean images.
I've had this happen on old BH focusing telescopes. The juice/oil...whatever solidifies over time.
This probably sounds ludicrous, but if you heat the unit in an over to about 100F, usually the stuck goo will let go. The key is to release the goo without damaging the optics.
The common solvent for the good seems to be acetone. But, in an heated environment, it will be gone quickly. So, you might have to try both approaches. Heat first and try to release then introduce acetone. Once released, put new oil on the threads. I have found that the best oil to date is Zeiss Uhrenol 40, INR:101.313 000000-0117-482-000 Clock/Watchmaker 1045.
This little bottle will last a lifetime. I have no idea if this is still available. If you cannot get it, I can send a few drops of this to you and that should serve you well into the future.
gary g.
At 09:31 AM 2/26/2009, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Feb 26 21:42:46 2009 11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1R3giiv021379 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 21:42:45 -0600 11, 20 -- Message-Id: {200902270342.n1R3giiv021379-at-ns.microscopy.com} 11, 20 -- Received: (qmail 14792 invoked from network); 26 Feb 2009 19:46:27 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 14789, pid: 14790, t: 0.1064s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp1 with SMTP; 26 Feb 2009 19:46:27 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Thu, 26 Feb 2009 19:42:34 -0800 11, 20 -- To: AJBowling-at-dow.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Phase telescope 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200902261731.n1QHVwG6013931-at-ns.microscopy.com} 11, 20 -- References: {200902261731.n1QHVwG6013931-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Ok, so I put the telescope in the oven at ~100F for an hour, just as Gary suggested, and this worked like a charm! I unscrewed it enough to expose some threads and I put a drop of Liquid Wrench with Teflon on them, worked it in a bit, and wiped off the liberated green goo with a kimwipe (I repeated this a few times). I tested it again after putting it in the fridge for a while to make sure that the grease wouldn't set when it cooled down, and it appears to be totally fixed. One final note, I had already tried to heat the telescope in the same oven set at 60 degrees and it didn't work. Heating it higher was definitely required.
Thanks!
Andy Bowling
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Thursday, February 26, 2009 10:49 PM To: Bowling, Andrew (AJ)
I've had this happen on old BH focusing telescopes. The juice/oil...whatever solidifies over time.
This probably sounds ludicrous, but if you heat the unit in an over to about 100F, usually the stuck goo will let go. The key is to release the goo without damaging the optics.
The common solvent for the good seems to be acetone. But, in an heated environment, it will be gone quickly. So, you might have to try both approaches. Heat first and try to release then introduce acetone. Once released, put new oil on the threads. I have found that the best oil to date is Zeiss Uhrenol 40, INR:101.313 000000-0117-482-000 Clock/Watchmaker 1045.
This little bottle will last a lifetime. I have no idea if this is still available. If you cannot get it, I can send a few drops of this to you and that should serve you well into the future.
gary g.
At 09:31 AM 2/26/2009, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Feb 26 21:42:46 2009 11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1R3giiv021379 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 21:42:45 -0600 11, 20 -- Message-Id: {200902270342.n1R3giiv021379-at-ns.microscopy.com} 11, 20 -- Received: (qmail 14792 invoked from network); 26 Feb 2009 19:46:27 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 14789, pid: 14790, t: 0.1064s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp1 with SMTP; 26 Feb 2009 19:46:27 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Thu, 26 Feb 2009 19:42:34 -0800 11, 20 -- To: AJBowling-at-dow.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Phase telescope 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200902261731.n1QHVwG6013931-at-ns.microscopy.com} 11, 20 -- References: {200902261731.n1QHVwG6013931-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 20, 33 -- From AJBowling-at-dow.com Fri Feb 27 14:05:30 2009 20, 33 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.242.179]) 20, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1RK5S2Q006669 20, 33 -- for {microscopy-at-microscopy.com} ; Fri, 27 Feb 2009 14:05:29 -0600 20, 33 -- X-VirusChecked: Checked 20, 33 -- X-Env-Sender: AJBowling-at-dow.com 20, 33 -- X-Msg-Ref: server-11.tower-86.messagelabs.com!1235765113!7036768!12 20, 33 -- X-StarScan-Version: 6.0.0; banners=-,-,- 20, 33 -- X-Originating-IP: [216.99.65.22] 20, 33 -- Received: (qmail 31310 invoked from network); 27 Feb 2009 20:05:26 -0000 20, 33 -- Received: from mail1.dow.com (HELO USMDLMDOWS001.dow.com) (216.99.65.22) 20, 33 -- by server-11.tower-86.messagelabs.com with RC4-SHA encrypted SMTP; 27 Feb 2009 20:05:26 -0000 20, 33 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USMDLMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830); 20, 33 -- Fri, 27 Feb 2009 15:05:25 -0500 20, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 33 -- Content-class: urn:content-classes:message 20, 33 -- MIME-Version: 1.0 20, 33 -- Content-Type: text/plain; 20, 33 -- charset="us-ascii" 20, 33 -- Subject: [Microscopy] Re: Phase telescope 20, 33 -- Date: Fri, 27 Feb 2009 15:05:24 -0500 20, 33 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1DA68F2-at-USMDLMDOWX032.dow.com} 20, 33 -- In-Reply-To: {200902270348.n1R3mcPD001894-at-ns.microscopy.com} 20, 33 -- X-MS-Has-Attach: 20, 33 -- X-MS-TNEF-Correlator: 20, 33 -- Thread-Topic: [Microscopy] Re: Phase telescope 20, 33 -- Thread-Index: AcmYjkhMpTymfnlBTUSLPw4w7yWfzgAaQIWg 20, 33 -- References: {200902270348.n1R3mcPD001894-at-ns.microscopy.com} 20, 33 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com} 20, 33 -- To: {microscopy-at-microscopy.com} 20, 33 -- X-OriginalArrivalTime: 27 Feb 2009 20:05:25.0052 (UTC) FILETIME=[BA4A1FC0:01C99916] 20, 33 -- Content-Transfer-Encoding: 8bit 20, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n1RK5S2Q006669 ==============================End of - Headers==============================
You are, of course, correct. I tried 60C first and got nothing, but then boosted the oven to around 100C and had success!
Good thing it's Friday!
andy b
________________________________
From: Straszheim, Warren E [M S E] [mailto:wesaia-at-iastate.edu] Sent: Friday, February 27, 2009 3:36 PM To: Bowling, Andrew (AJ) Subject: RE: [Microscopy] Re: Phase telescope
What were those temperatures? You say you tried 60 degrees. That must have been C, because 60F is below ambient around here, at least when I have my furnace running.
60C = 140F and that is warmer than what Gary suggested.
However, if it works, that is the important thing. You may just want to clarify it for the rest of us for future reference.
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Ok, so I put the telescope in the oven at ~100F for an hour, just as Gary suggested, and this worked like a charm! I unscrewed it enough to expose some threads and I put a drop of Liquid Wrench with Teflon on them, worked it in a bit, and wiped off the liberated green goo with a kimwipe (I repeated this a few times). I tested it again after putting it in the fridge for a while to make sure that the grease wouldn't set when it cooled down, and it appears to be totally fixed. One final note, I had already tried to heat the telescope in the same oven set at 60 degrees and it didn't work. Heating it higher was definitely required.
Thanks!
Andy Bowling
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Thursday, February 26, 2009 10:49 PM To: Bowling, Andrew (AJ) Subject: [Microscopy] Re: Phase telescope
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I've had this happen on old BH focusing telescopes. The juice/oil...whatever solidifies over time.
This probably sounds ludicrous, but if you heat the unit in an over to about 100F, usually the stuck goo will let go. The key is to release the goo without damaging the optics.
The common solvent for the good seems to be acetone. But, in an heated environment, it will be gone quickly. So, you might have to try both approaches. Heat first and try to release then introduce acetone. Once released, put new oil on the threads. I have found that the best oil to date is Zeiss Uhrenol 40, INR:101.313 000000-0117-482-000 Clock/Watchmaker 1045.
This little bottle will last a lifetime. I have no idea if this is still available. If you cannot get it, I can send a few drops of this to you and that should serve you well into the future.
gary g.
At 09:31 AM 2/26/2009, you wrote:
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} ----------------------------------------------------------------------- ----- } } I have an Olympus centering telescope for a BH-2 phase contrast } microscope that has frozen. Has anyone had any luck getting one of these } un-stuck? } } Thanks, } } Andy Bowling } } } ==============================Original Headers============================== } 4, 31 -- From AJBowling-at-dow.com Thu Feb 26 11:30:32 2009 } 4, 31 -- Received: from mail164.messagelabs.com } (mail164.messagelabs.com [216.82.253.131]) } 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n1QHUViJ011530 } 4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 26 Feb 2009 } 11:30:31 -0600 } 4, 31 -- X-VirusChecked: Checked } 4, 31 -- X-Env-Sender: AJBowling-at-dow.com } 4, 31 -- X-Msg-Ref: server-4.tower-164.messagelabs.com!1235669425!16997483!5 } 4, 31 -- X-StarScan-Version: 6.0.0; banners=-,-,- } 4, 31 -- X-Originating-IP: [204.136.184.21] } 4, 31 -- Received: (qmail 12635 invoked from network); 26 Feb 2009 } 17:30:30 -0000 } 4, 31 -- Received: from mail3.dow.com (HELO USFRPMDOWS001.dow.com) } (204.136.184.21) } 4, 31 -- by server-4.tower-164.messagelabs.com with RC4-SHA } encrypted SMTP; 26 Feb 2009 17:30:30 -0000 } 4, 31 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by } USFRPMDOWS001.dow.com with Microsoft SMTPSVC(6.0.3790.1830); } 4, 31 -- Thu, 26 Feb 2009 11:30:27 -0600 } 4, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 4, 31 -- Content-class: urn:content-classes:message } 4, 31 -- MIME-Version: 1.0 } 4, 31 -- Content-Type: text/plain; } 4, 31 -- charset="us-ascii" } 4, 31 -- Subject: Phase telescope } 4, 31 -- Date: Thu, 26 Feb 2009 12:30:26 -0500 } 4, 31 -- Message-ID: } {B72477374D7A74408DAC63801A0FDEA1DA6474-at-USMDLMDOWX032.dow.com} } 4, 31 -- X-MS-Has-Attach: } 4, 31 -- X-MS-TNEF-Correlator: } 4, 31 -- Thread-Topic: Phase telescope } 4, 31 -- Thread-Index: AcmYN+lCKx0Et6jfSY2nPb6qKQH1GA== } 4, 31 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com} } 4, 31 -- To: {microscopy-at-microscopy.com} } 4, 31 -- X-OriginalArrivalTime: 26 Feb 2009 17:30:27.0673 (UTC)
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Feb 26 21:42:46 2009 11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)
Whoa, I said 100F, not C. That would over 200F Did you do F or C?
Like I said, the trick is to heat it up to loosen the goo without damaging the optics. Usually 100F works. If not, then about 125F. If that does not work, then I put methanol around the periphery to get it into the threads. Let it set for a day and then do the oven at 100F. If that still does not work, then I try to get acetone into the threads and back to the over after a day. So far, along this sequence, somewhere, the fix works.
I have not had to do this on phase units. But the problem is still the same--hard goo that used to be grease or oil.
gary g.
At 02:04 PM 2/27/2009, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Fri Feb 27 18:32:43 2009 10, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n1S0WeRR013235 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Feb 2009 18:32:41 -0600 10, 20 -- Message-Id: {200902280032.n1S0WeRR013235-at-ns.microscopy.com} 10, 20 -- Received: (qmail 26885 invoked from network); 27 Feb 2009 16:36:30 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 26879, pid: 26881, t: 0.2924s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 20 -- by smtp1 with SMTP; 27 Feb 2009 16:36:30 -0800 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Fri, 27 Feb 2009 16:32:30 -0800 10, 20 -- To: AJBowling-at-dow.com 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Re: Phase telescope 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200902272204.n1RM49kL028253-at-ns.microscopy.com} 10, 20 -- References: {200902272204.n1RM49kL028253-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
As with all EDAX's modern SiLi detectors, it was designed to automatically turn itself off when the LN2 was gone and the unit started warming up. The CDU can also cool down and stabilize very quickly, making it practical to leave at room temperature when not in use.
Bottom line: things are going to vary according to detector design and manufacturer. Your best bet is going to be contacting the company which made the detector and ask them.
Jeff Gschwend
On Feb 25, 2009, at 1:21 PM, bozzola-at-siu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This thread brought up a question. } } Suppose someone wants to mothball a LN2 chilled detector for a } period of time. } } Is this feasible without significant damage? } } What precautions should be taken (bias off and voltage drained) prior } to warming up, etc? } } What are the negative aspects of doing this (loss of resolution, } presumably)? } } Thanks for the information. } -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } Integrated Microscopy & Graphics Expertise (IMAGE) } Southern Illinois University } 750 Communications Drive - MC 4402 } Carbondale, IL 62901 } Telephone: 618-453-3730 } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } ==============================Original } Headers============================== } 8, 19 -- From bozzola-at-siu.edu Wed Feb 25 13:19:55 2009 } 8, 19 -- Received: from cstmta2.siu.edu (cstmta2.siu.edu } [131.230.1.2]) } 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n1PJJtov024308 } 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:55 } -0600 } 8, 19 -- Received: from [131.230.177.136] } (ws177136.microscope.siu.edu [131.230.177.136]) } 8, 19 -- by cstmta2.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP } id n1PJJrDS001332 } 8, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Feb 2009 13:19:54 } -0600 (CST) } 8, 19 -- Mime-Version: 1.0 } 8, 19 -- Message-Id: {a06240802c5cb492177ae-at-[131.230.177.136]} } 8, 19 -- In-Reply-To: {200902251738.n1PHcCHW025499-at-ns.microscopy.com} } 8, 19 -- References: {200902251738.n1PHcCHW025499-at-ns.microscopy.com} } 8, 19 -- Date: Wed, 25 Feb 2009 13:19:52 -0600 } 8, 19 -- To: Microscopy-at-microscopy.com } 8, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 8, 19 -- Subject: [Microscopy] Another EDX detector question } 8, 19 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } 8, 19 -- X-Spam-Score: 0.00% } 8, 19 -- X-MASF: 0.00% } 8, 19 -- X-Whitelist: 0.00% } ==============================End of - } Headers==============================
==============================Original Headers============================== 11, 24 -- From jgschwen-at-rcn.com Sat Feb 28 10:52:54 2009 11, 24 -- Received: from smtp02.lnh.mail.rcn.net (smtp02.lnh.mail.rcn.net [207.172.157.102]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n1SGqs9D025986 11, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 28 Feb 2009 10:52:54 -0600 11, 24 -- Received: from mr02.lnh.mail.rcn.net ([207.172.157.22]) 11, 24 -- by smtp02.lnh.mail.rcn.net with ESMTP; 28 Feb 2009 11:52:54 -0500 11, 24 -- Received: from smtp01.lnh.mail.rcn.net (smtp01.lnh.mail.rcn.net [207.172.4.11]) 11, 24 -- by mr02.lnh.mail.rcn.net (MOS 3.10.4-GA) 11, 24 -- with ESMTP id POF69152; 11, 24 -- Sat, 28 Feb 2009 11:52:48 -0500 (EST) 11, 24 -- X-Auth-ID: jgschwen 11, 24 -- Received: from ip-69-33-68-33.chi.megapath.net ([69.33.68.33]) 11, 24 -- by smtp01.lnh.mail.rcn.net with ESMTP; 28 Feb 2009 11:52:49 -0500 11, 24 -- Message-Id: {F77F6994-DDF7-405C-A7C5-47B15851FCD8-at-rcn.com} 11, 24 -- From: Jeff Gschwend {jgschwen-at-rcn.com} 11, 24 -- To: Microscopy-at-microscopy.com 11, 24 -- In-Reply-To: {200902251921.n1PJL6qm026803-at-ns.microscopy.com} 11, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 11, 24 -- Content-Transfer-Encoding: 7bit 11, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 24 -- Subject: Re: [Microscopy] Another EDX detector question 11, 24 -- Date: Sat, 28 Feb 2009 10:52:47 -0600 11, 24 -- References: {200902251921.n1PJL6qm026803-at-ns.microscopy.com} 11, 24 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Re microscopy programs: we also offer a great, new certificate in bioscience microscopy at Merritt College in Oakland. The focus (pun intended) is on fluorescence, including confocal microscopy. It's a one year program, night and weekends, and includes an internship and a practicum. We have over 2 million dollars of scopes, all sorts of systems and software, including two confocals, and a tissue culture facility. Our instructors all have bio PhDs, including myself (and I took the Woods Hole intensive in microscopy). We're a community college so the cost is only $20/unit! We currently have 70 students in the program and the first group is graduating and on the job market. Check it out at: www.merritt.edu/microscopy
On a side note: we're thinking of setting up mini-courses for folks already working in the field. Please let me know if there's interest and what topics would be prefered!
We're also open to leasing time on our scopes, in return for taking on a student as an intern (or just for a small fee instead)!
Also: graduation of the first group of students is Sat, March 28th, 6-10pm. All are welcome: it's a great chance to get to know the program. The students will talk about their experiences, and there'll be food and live music. It's in the student lounge, R building of the campus: just come!
Last, but not least, if you need to hire a brilliant, motivated, enthusiastic, hard-working, well-training microscopist, please let me know! Our students are trained in both the theory and practice of microscopy, and they have lots of hands-on experience. They're great at ciritical thinking, troubleshooting, and juggling multiple demands. Many had previous careers in computers, management, graphics, photography, etc.
regards, Gisele Giorgi, PhD Director, Merritt Microscopy Program Merritt College 12500 Campus Drive Oakland, CA 94619 510-436-2618 ggiorgi-at-peralta.edu www.merritt.edu/microscopy
At 7:56 AM -0600 11/24/08, zaluzec-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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The EDAX units automatically shut off if the detector tip temperature is not at spec. This prompts a call to their service folks. They are Johnny on the spot.
From my experience, letting EDAX or legacy Rontec (UHV) detectors go dry for extended periods of time makes no difference. However, the Rontec UHV units have wimpy Dewars. So, that is the way that they are. But they work. They got bought up by some other company...nothing new about this.
So you are right about contacting and getting credible responses (problematic) from the company. For new procurements, a list of specific requirements ought to be very helpful.
Disclaimer: No financial interest in EDAX/TSL or Ametek other than being a very satisfied customer.
Dr. Gary Gaugler
At 08:54 AM 2/28/2009, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Sat Feb 28 22:21:41 2009 13, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n214LeR3002139 13, 20 -- for {microscopy-at-microscopy.com} ; Sat, 28 Feb 2009 22:21:40 -0600 13, 20 -- Message-Id: {200903010421.n214LeR3002139-at-ns.microscopy.com} 13, 20 -- Received: (qmail 30220 invoked from network); 28 Feb 2009 20:25:43 -0800 13, 20 -- Received: by simscan 1.1.0 ppid: 30217, pid: 30218, t: 0.1475s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 13, 20 -- by smtp1 with SMTP; 28 Feb 2009 20:25:43 -0800 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Sat, 28 Feb 2009 20:21:37 -0800 13, 20 -- To: jgschwen-at-rcn.com 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] Re: Another EDX detector question 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200902281654.n1SGsel2028385-at-ns.microscopy.com} 13, 20 -- References: {200902281654.n1SGsel2028385-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
For more years than I care to remember, I have been attending the M and M annual meeting. Typically, I have come home each year with at least one tote bag – gifts from the manufacturers. These bags have been squirreled away (pack rat that I am) in a pile in a closet, causing me to wonder why I continue to accept these gifts.
Recently during Spring cleaning looking at the pile of many bags, it occurred to me that these bags are very similar to the bags that are now on sale in every supermarket, to reduce greenhouse gases through not using plastic bags. So I have started using M and M tote bags when I go to the supermarket. They are great.
Am I the last to realize this? Has everyone else been doing this for ages?
-- Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 4, 21 -- From jae5-at-lehigh.edu Sun Mar 1 11:22:19 2009 4, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n21HMJSC025229 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 11:22:19 -0600 4, 21 -- Received: from [192.168.1.101] (70.15.7.77.res-cmts.sm.ptd.net [70.15.7.77]) 4, 21 -- (authenticated bits=0) 4, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n21HMGc0017150 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 12:22:18 -0500 4, 21 -- Message-ID: {49AAC445.2060709-at-lehigh.edu} 4, 21 -- Date: Sun, 01 Mar 2009 12:22:13 -0500 4, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 4, 21 -- Reply-To: jae5-at-lehigh.edu 4, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 4, 21 -- MIME-Version: 1.0 4, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 4, 21 -- Subject: Tote bags 4, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 21 -- Content-Transfer-Encoding: 8bit 4, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on rain.cc.lehigh.edu 4, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
It is the sole reason that I collect them. I found that I had to shorten the handles on the big white Zeiss bags, and due to their size I have to instruct the bagger to put only the lightweight items in them
On Sun Mar 01 12:23:12 EST 2009, jae5-at-lehigh.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } For more years than I care to remember, I have been attending the } M and M annual meeting. Typically, I have come home each year } with at least one tote bag ?? gifts from the manufacturers. } These bags have been squirreled away (pack rat that I am) in a } pile in a closet, causing me to wonder why I continue to accept } these gifts. } } Recently during Spring cleaning looking at the pile of many bags, } it occurred to me that these bags are very similar to the bags } that are now on sale in every supermarket, to reduce greenhouse } gases through not using plastic bags. So I have started using M } and M tote bags when I go to the supermarket. They are great. } } Am I the last to realize this? Has everyone else been doing this } for ages? } } -- Alwyn Eades } Department of Materials Science and Engineering } Lehigh University } 5 East Packer Avenue } Bethlehem } Pennsylvania 18015-3195 } Phone 610 758 4231 } Fax 610 758 4244 } jae5-at-lehigh.edu } ==============================Original } Headers============================== } 4, 21 -- From jae5-at-lehigh.edu Sun Mar 1 11:22:19 2009 } 4, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu } [128.180.2.160]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n21HMJSC025229 } 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 } 11:22:19 -0600 } 4, 21 -- Received: from [192.168.1.101] } (70.15.7.77.res-cmts.sm.ptd.net [70.15.7.77]) } 4, 21 -- (authenticated bits=0) } 4, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id } n21HMGc0017150 } 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NOT) } 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 } 12:22:18 -0500 } 4, 21 -- Message-ID: {49AAC445.2060709-at-lehigh.edu} } 4, 21 -- Date: Sun, 01 Mar 2009 12:22:13 -0500 } 4, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} } 4, 21 -- Reply-To: jae5-at-lehigh.edu } 4, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- To: "MicroscopyListserver (E-mail)" } {Microscopy-at-microscopy.com} } 4, 21 -- Subject: Tote bags } 4, 21 -- Content-Type: text/plain; charset=windows-1252; } format=flowed } 4, 21 -- Content-Transfer-Encoding: 8bit } 4, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter } version 0.94.2 on rain.cc.lehigh.edu } 4, 21 -- X-Virus-Status: Clean } ==============================End of - } Headers============================== } }
-- Greg Erdos Assistant Director Emeritus Micanopy FL
We have recently taken delivery of a new hot stage for our TEM. We are interested in understanding the likely accuracy of the temperature read out.
In previous hot stage work, I have simply accepted the indicated temperatures and put a 'reality factor' of +/-30deg C through it. This is a reflection of poor thermal conductivity (for carbon support films at lower temperatures {500degC), surface energy, residual stress, beam heating and specimen preparation factors affecting the temperature at which phase transformations, melting, recrystallisation etc occurs in the TEM.
However, we have some folk here who are interested in accurate temperature work and so if anyone has developed or used readily available materials as temperature calibration materials, I'd be interested in hearing from you.
Thanks and regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
==============================Original Headers============================== 15, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 1 17:19:26 2009 15, 24 -- Received: from sanaa.ucc.usyd.edu.au (sanaa.ucc.usyd.edu.au [129.78.64.145]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n21NJNFx001817 15, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 17:19:24 -0600 15, 24 -- Received: from sanaa.ucc.usyd.edu.au (localhost [127.0.0.1]) 15, 24 -- by localhost (Postfix) with SMTP id 359F01775AA 15, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 10:19:21 +1100 (EST) 15, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19]) 15, 24 -- by sanaa.ucc.usyd.edu.au (Postfix) with ESMTP id 28A131775A7 15, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 10:19:21 +1100 (EST) 15, 24 -- Received: from 172.17.185.136 ([172.17.185.136]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server HTTP-DAV ; 15, 24 -- Sun, 1 Mar 2009 23:19:20 +0000 15, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 15, 24 -- Date: Mon, 02 Mar 2009 10:19:20 +1100 15, 24 -- Subject: TEM: Hot Stage Calibration 15, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au} 15, 24 -- To: {Microscopy-at-microscopy.com} 15, 24 -- Message-ID: {C5D16328.34C%david.mitchell-at-emu.usyd.edu.au} 15, 24 -- Thread-Topic: TEM: Hot Stage Calibration 15, 24 -- Thread-Index: AcmaxCYVZJVpLAa3Ed6onQAjMrpsqg== 15, 24 -- Mime-version: 1.0 15, 24 -- Content-type: text/plain; 15, 24 -- charset="US-ASCII" 15, 24 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Every year there is a local drive here in Santa Clara to donate backpacks to students who cannot afford to purchase their own. I always donate my MSA backpacks to students. It gets them a free backpack and advertises Microscopy to everyone in the schools. I would hope that a lot of MSA members would be able to make use of this way off finding a second life for MSA backpacks as well.
John Mardinly, Numonyx
-----Original Message----- X-from: gwe-at-ufl.edu [mailto:gwe-at-ufl.edu] Sent: Sunday, March 01, 2009 9:34 AM To: MARDINLY, A
It is the sole reason that I collect them. I found that I had to shorten the handles on the big white Zeiss bags, and due to their size I have to instruct the bagger to put only the lightweight items in them
On Sun Mar 01 12:23:12 EST 2009, jae5-at-lehigh.edu wrote:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } For more years than I care to remember, I have been attending the } M and M annual meeting. Typically, I have come home each year } with at least one tote bag ?? gifts from the manufacturers. } These bags have been squirreled away (pack rat that I am) in a } pile in a closet, causing me to wonder why I continue to accept } these gifts. } } Recently during Spring cleaning looking at the pile of many bags, } it occurred to me that these bags are very similar to the bags } that are now on sale in every supermarket, to reduce greenhouse } gases through not using plastic bags. So I have started using M } and M tote bags when I go to the supermarket. They are great. } } Am I the last to realize this? Has everyone else been doing this } for ages? } } -- Alwyn Eades } Department of Materials Science and Engineering } Lehigh University } 5 East Packer Avenue } Bethlehem } Pennsylvania 18015-3195 } Phone 610 758 4231 } Fax 610 758 4244 } jae5-at-lehigh.edu } ==============================Original } Headers============================== } 4, 21 -- From jae5-at-lehigh.edu Sun Mar 1 11:22:19 2009 } 4, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu } [128.180.2.160]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n21HMJSC025229 } 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 } 11:22:19 -0600 } 4, 21 -- Received: from [192.168.1.101] } (70.15.7.77.res-cmts.sm.ptd.net [70.15.7.77]) } 4, 21 -- (authenticated bits=0) } 4, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id } n21HMGc0017150 } 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NOT) } 4, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Mar 2009 } 12:22:18 -0500 } 4, 21 -- Message-ID: {49AAC445.2060709-at-lehigh.edu} } 4, 21 -- Date: Sun, 01 Mar 2009 12:22:13 -0500 } 4, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} } 4, 21 -- Reply-To: jae5-at-lehigh.edu } 4, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- To: "MicroscopyListserver (E-mail)" } {Microscopy-at-microscopy.com} } 4, 21 -- Subject: Tote bags } 4, 21 -- Content-Type: text/plain; charset=windows-1252; } format=flowed } 4, 21 -- Content-Transfer-Encoding: 8bit } 4, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter } version 0.94.2 on rain.cc.lehigh.edu } 4, 21 -- X-Virus-Status: Clean } ==============================End of - } Headers============================== } }
-- Greg Erdos Assistant Director Emeritus Micanopy FL
Hello We have a FEI XL30 ESEM-FEG dating back a few years now and are beginning to encounter problems with sourcing spare parts. Just wondering if anyone else out there is running an xl30 esem and if so are you having similar problems now they are no longer made,or does everyone have a Quanta these days?
Thanks Nikki Weston School M3 University of Nottingham
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==============================Original Headers============================== 5, 32 -- From Nicola.Weston-at-nottingham.ac.uk Mon Mar 2 04:05:01 2009 5, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22A4ve7017438 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 04:04:58 -0600 5, 32 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 5, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 5, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 5, 32 -- id 1Le4yO-0007mK-P5 5, 32 -- for microscopy-at-microscopy.com; Mon, 02 Mar 2009 10:02:08 +0000 5, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 5, 32 -- Mon, 2 Mar 2009 10:01:48 +0000 5, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 5, 32 -- Content-class: urn:content-classes:message 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Type: text/plain; 5, 32 -- charset="us-ascii" 5, 32 -- Subject: ESEM users 5, 32 -- Date: Mon, 2 Mar 2009 10:01:48 -0000 5, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94EF23C7-at-VUIEXCHA.ad.nottingham.ac.uk} 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- Thread-Topic: ESEM users 5, 32 -- Thread-Index: AcmbHeakr1F3obFISgOwE1S60jpp0A== 5, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 5, 32 -- To: {microscopy-at-microscopy.com} 5, 32 -- X-OriginalArrivalTime: 02 Mar 2009 10:01:48.0940 (UTC) FILETIME=[E70B3CC0:01C99B1D] 5, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 5, 32 -- X-UoN-MailScanner: Found to be clean 5, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 5, 32 -- X-Spam-Status: No 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22A4ve7017438 ==============================End of - Headers==============================
Hi Nikki, no problems with our tungsten gun XL30 ESEM re support so far.
On a related theme we were worrying about our Philips CM10 TEM which ceases to be supported, I believe, in 2011. Unofficially should be able to keep them going for a long time as there is a large customer base in the UK.
Dave
-----Original Message----- X-from: Nicola.Weston-at-nottingham.ac.uk [mailto:Nicola.Weston-at-nottingham.ac.uk] Sent: 02 March 2009 10:19 To: David Patton
Hello We have a FEI XL30 ESEM-FEG dating back a few years now and are beginning to encounter problems with sourcing spare parts. Just wondering if anyone else out there is running an xl30 esem and if so are you having similar problems now they are no longer made,or does everyone have a Quanta these days?
Thanks Nikki Weston School M3 University of Nottingham
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==============================Original Headers============================== 5, 32 -- From Nicola.Weston-at-nottingham.ac.uk Mon Mar 2 04:05:01 2009 5, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22A4ve7017438 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 04:04:58 -0600 5, 32 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 5, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 5, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 5, 32 -- id 1Le4yO-0007mK-P5 5, 32 -- for microscopy-at-microscopy.com; Mon, 02 Mar 2009 10:02:08 +0000 5, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 5, 32 -- Mon, 2 Mar 2009 10:01:48 +0000 5, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 5, 32 -- Content-class: urn:content-classes:message 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Type: text/plain; 5, 32 -- charset="us-ascii" 5, 32 -- Subject: ESEM users 5, 32 -- Date: Mon, 2 Mar 2009 10:01:48 -0000 5, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94EF23C7-at-VUIEXCHA.ad.nottingham.ac.uk} 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- Thread-Topic: ESEM users 5, 32 -- Thread-Index: AcmbHeakr1F3obFISgOwE1S60jpp0A== 5, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 5, 32 -- To: {microscopy-at-microscopy.com} 5, 32 -- X-OriginalArrivalTime: 02 Mar 2009 10:01:48.0940 (UTC) FILETIME=[E70B3CC0:01C99B1D] 5, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 5, 32 -- X-UoN-MailScanner: Found to be clean 5, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 5, 32 -- X-Spam-Status: No 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22A4ve7017438 ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 34 -- From David.Patton-at-uwe.ac.uk Mon Mar 2 04:59:45 2009 18, 34 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65]) 18, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n22Axiin032408 18, 34 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 04:59:45 -0600 18, 34 -- Received: from (unknown [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp 18, 34 -- id 2346_3c61125a_0719_11de_b5bc_00142221cca9; 18, 34 -- Mon, 02 Mar 2009 10:59:43 +0000 18, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 18, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 18, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 18, 34 -- 2005)) with SMTP id {0KFV00AA2L7JLD-at-mta02.uwe.ac.uk} for 18, 34 -- microscopy-at-microscopy.com; Mon, 02 Mar 2009 10:59:43 +0000 (GMT) 18, 34 -- Date: Mon, 02 Mar 2009 10:58:41 +0000 18, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 18, 34 -- Subject: RE: [Microscopy] ESEM users & TEMs after official support ends! 18, 34 -- In-reply-to: {200903021019.n22AJFVq023443-at-ns.microscopy.com} 18, 34 -- To: microscopy-at-microscopy.com 18, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB046778D5-at-egen-uwe01} 18, 34 -- MIME-version: 1.0 18, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 18, 34 -- Content-type: text/plain; charset=us-ascii 18, 34 -- Content-class: urn:content-classes:message 18, 34 -- Thread-topic: [Microscopy] ESEM users & TEMs after official support ends! 18, 34 -- Thread-index: AcmbIF09/sklQly5RVyM8jz7jRHMZAABK9IQ 18, 34 -- X-MS-Has-Attach: 18, 34 -- X-MS-TNEF-Correlator: 18, 34 -- References: {200903021019.n22AJFVq023443-at-ns.microscopy.com} 18, 34 -- X-NAIMIME-Disclaimer: 1 18, 34 -- X-NAIMIME-Modified: 1 18, 34 -- X-NAI-Spam-Score: 0 18, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 18, 34 -- RV3221=0 18, 34 -- Content-Transfer-Encoding: 8bit 18, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22Axiin032408 ==============================End of - Headers==============================
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Email: y.han-at-sheffield.ac.uk Name: Yisong Han
Organization: University of Sheffield
Title-Subject: [Filtered] Sample drifting in TEM
Question: Dear All,
I had a chance to look at a bulk ceramic which contains Pb, Mg and W. I knew it has a phase transition temperature at around 40 degree C. I indeed saw domain movement with the electron beam and I guess the phase transition occurred due to a heating effect by the beam and I was not surprised about this.
But I still noticed that the shadow of the objective aperture moved around a lot while I was moving the sample. Also the diffraction spots were highly distorted from what we normally see and this could not be corrected by a combined adjustment of C2 and diffraction focus. I know this sample should be cooled or heated to avoid such a transition taking place. I was wondering if anybody has encountered such a situation and can give an explanation about the drifting of the objective aperture and distortion of diffraction spots. Is this material is slightly magnetic or something else... Thanks very much for your attention.
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==============================Original Headers============================== 3, 27 -- From oshel1pe-at-cmich.edu Mon Mar 2 08:31:00 2009 3, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22EUxhO032705 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 08:30:59 -0600 3, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n22EUsnA022194 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 09:30:55 -0500 3, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 27 -- Mon, 2 Mar 2009 09:30:38 -0500 3, 27 -- Mime-Version: 1.0 3, 27 -- Message-Id: {f06240803c5d19dbdc491-at-[141.209.160.249]} 3, 27 -- In-Reply-To: {200903011726.n21HQwRW031799-at-ns.microscopy.com} 3, 27 -- References: {200903011726.n21HQwRW031799-at-ns.microscopy.com} 3, 27 -- Date: Mon, 2 Mar 2009 09:30:36 -0500 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 27 -- Subject: Re: [Microscopy] Tote bags 3, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 3, 27 -- X-OriginalArrivalTime: 02 Mar 2009 14:30:38.0532 (UTC) FILETIME=[7507A840:01C99B43] 3, 27 -- X-Canit-CHI2: 0.00 3, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 27 -- X-Spam-Score: -2.60 () [Hold at 5.00] L_EXCH_MF,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 3, 27 -- X-CanItPRO-Stream: default 3, 27 -- X-Canit-Stats-ID: 9685874 - 6d29878c9cbe 3, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 3, 27 -- Content-Transfer-Encoding: 8bit 3, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22EUxhO032705 ==============================End of - Headers==============================
Hi Yisong, is your sample ferroelectric? I think you have problems with sample charging; I have seen similar things myself recently. The interaction of charge on the sample and the spontaneous ferroelectric charge in the material can certainly cause domain walls to move. I have found that plasma cleaning of ceramic TEM samples may get rid of contamination, but can make them impossible to work with because of this. If you didn't plasma clean your specimen, try a light carbon coat on both sides of the specimen to reduce charging and try to keep the beam current density low.
Also, given the level of complexity that can occur in some oxides your 'distorted spots' may actually be showing real things, such as modulations in composition ('tweed' structure, defects, ordered oxygen vacancies, etc. etc. etc. etc.)
Good luck
Richard Beanland
y.han-at-sheffield.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both y.han-at-sheffield.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: y.han-at-sheffield.ac.uk } Name: Yisong Han } } Organization: University of Sheffield } } Title-Subject: [Filtered] Sample drifting in TEM } } Question: Dear All, } } I had a chance to look at a bulk ceramic which contains Pb, Mg and W. } I knew it has a phase transition temperature at around 40 degree C. I } indeed saw domain movement with the electron beam and I guess the } phase transition occurred due to a heating effect by the beam and I } was not surprised about this. } } But I still noticed that the shadow of the objective aperture moved } around a lot while I was moving the sample. Also the diffraction } spots were highly distorted from what we normally see and this could } not be corrected by a combined adjustment of C2 and diffraction } focus. I know this sample should be cooled or heated to avoid such a } transition taking place. I was wondering if anybody has encountered } such a situation and can give an explanation about the drifting of } the objective aperture and distortion of diffraction spots. Is this } material is slightly magnetic or something else... Thanks very much } for your attention. } } Yisong } } Login Host: 143.167.204.40 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Mon Mar 2 08:16:10 2009 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22EG9L6021109 } 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 08:16:10 -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240803c5d19a9525ba-at-[206.69.208.22]} } 9, 11 -- Date: Mon, 2 Mar 2009 08:16:04 -0600 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: y.han-at-sheffield.ac.uk (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Sample drifting in TEM } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 6, 29 -- From contact-at-integrityscientific.com Mon Mar 2 09:06:00 2009 6, 29 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22F5wwt017408 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 09:05:59 -0600 6, 29 -- Received: from localhost (localhost [127.0.0.1]) 6, 29 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n22F5vAV007516; 6, 29 -- Mon, 2 Mar 2009 15:05:57 GMT 6, 29 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 6, 29 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1]) 6, 29 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 6, 29 -- with LMTP id LxcXA9wBo4SV; Mon, 2 Mar 2009 15:05:52 +0000 (GMT) 6, 29 -- Received: from [137.205.164.171] (hosts-137-205-164-171 [137.205.164.171]) 6, 29 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id n22F5NCU007318; 6, 29 -- Mon, 2 Mar 2009 15:05:23 GMT 6, 29 -- X-Envelope-From: contact-at-integrityscientific.com 6, 29 -- Message-ID: {49ABF59B.3060704-at-integrityscientific.com} 6, 29 -- Date: Mon, 02 Mar 2009 15:04:59 +0000 6, 29 -- From: Richard Beanland {contact-at-integrityscientific.com} 6, 29 -- Reply-To: contact-at-integrityscientific.com 6, 29 -- Organization: Integrity Scientific Ltd 6, 29 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 6, 29 -- MIME-Version: 1.0 6, 29 -- To: y.han-at-sheffield.ac.uk, microscopy-at-microscopy.com 6, 29 -- Subject: Re: [Microscopy] viaWWW: Sample drifting in TEM 6, 29 -- References: {200903021429.n22ETQ5w031000-at-ns.microscopy.com} 6, 29 -- In-Reply-To: {200903021429.n22ETQ5w031000-at-ns.microscopy.com} 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-DCC-Warwick-Metrics: nightshade; whitelist ==============================End of - Headers==============================
I am uncertain what you mean by "ceases to be supported".
My understanding is that there are several possible meanings: 1. The supplier will not offer service contracts or repairs. 2. The supplier will be disposing of all specific spares but still do contracts and repairs eg if you have parts. 3. The supplier will no longer be re-stocking specific parts but will hold a diminishing stock and do service repairs.
Obviously if statement 2 or 3 is true then the machine could still be serviced by the supplier providing you or they have the parts. So it is useful to know which is true.
I don't know if this helps but it may give you an extra year or two.
Malcolm
Malcolm Haswell e.m. unit The Faculty of Applied Sciences University of Sunderland SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: David.Patton-at-uwe.ac.uk
Dear All, does anyone have or know where I can find procedures for Osmium staining of polymeric materials?
Thanks in advance for your help.
Danny King Test Chemist A123 Systems 3850 Research Park Dr. Ann Arbor, MI 48108
==============================Original Headers============================== 4, 26 -- From dking-at-a123systems.com Mon Mar 2 11:20:59 2009 4, 26 -- Received: from us-bos-mail2.a123systems.com (mail.a123systems.com [208.58.17.130]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22HKx1m017509 4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 11:20:59 -0600 4, 26 -- Received: from US-BOS-MAIL1.a123systems.com ([172.16.10.226]) by us-bos-mail2.a123systems.com with Microsoft SMTPSVC(6.0.3790.3959); 4, 26 -- Mon, 2 Mar 2009 12:19:07 -0500 4, 26 -- Received: from US-BOS-MAIL1.a123systems.com ([::1]) by 4, 26 -- US-BOS-MAIL1.a123systems.com ([fe80::5efe:172.16.10.226%12]) with mapi; Mon, 4, 26 -- 2 Mar 2009 12:19:07 -0500 4, 26 -- From: Danny King {dking-at-a123systems.com} 4, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 26 -- Date: Mon, 2 Mar 2009 12:19:06 -0500 4, 26 -- Subject: Os Staining to Contrast Polymer in SEM 4, 26 -- Thread-Topic: Os Staining to Contrast Polymer in SEM 4, 26 -- Thread-Index: AcmbWv3yZAG4nHhzSByexVmpD786mw== 4, 26 -- Message-ID: {9D7A68726BF97948A16CD4BF00DD249D02D51A94FD-at-US-BOS-MAIL1.a123systems.com} 4, 26 -- Accept-Language: en-US 4, 26 -- Content-Language: en-US 4, 26 -- X-MS-Has-Attach: 4, 26 -- X-MS-TNEF-Correlator: 4, 26 -- acceptlanguage: en-US 4, 26 -- Content-Type: text/plain; charset="us-ascii" 4, 26 -- MIME-Version: 1.0 4, 26 -- X-OriginalArrivalTime: 02 Mar 2009 17:19:07.0588 (UTC) FILETIME=[FE7F3040:01C99B5A] 4, 26 -- Content-Transfer-Encoding: 8bit 4, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22HKx1m017509 ==============================End of - Headers==============================
Sent: Monday, March 02, 2009 5:16 AM To: lherault-at-bu.edu
Hello We have a FEI XL30 ESEM-FEG dating back a few years now and are beginning to encounter problems with sourcing spare parts. Just wondering if anyone else out there is running an xl30 esem and if so are you having similar problems now they are no longer made,or does everyone have a Quanta these days?
Thanks Nikki Weston
==============================Original Headers============================== 11, 22 -- From lherault-at-bu.edu Mon Mar 2 11:29:02 2009 11, 22 -- Received: from vms173001pub.verizon.net (vms173001pub.verizon.net [206.46.173.1]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22HT2pN030854 11, 22 -- for {microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 11:29:02 -0600 11, 22 -- Received: from ronlherault ([71.162.90.228]) by vms173001.mailsrvcs.net 11, 22 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 11, 22 -- with ESMTPA id {0KFW00GNX385N3HZ-at-vms173001.mailsrvcs.net} for 11, 22 -- microscopy-at-microscopy.com; Mon, 02 Mar 2009 11:28:55 -0600 (CST) 11, 22 -- Reply-to: {lherault-at-bu.edu} 11, 22 -- From: "Ron L'Herault" {lherault-at-bu.edu} 11, 22 -- To: {microscopy-at-microscopy.com} 11, 22 -- References: {200903021016.n22AGCEg022238-at-ns.microscopy.com} 11, 22 -- Subject: RE: [Microscopy] ESEM users 11, 22 -- Date: Mon, 02 Mar 2009 12:28:51 -0500 11, 22 -- Message-id: {884189E80C084796A9A0727B16C15197-at-ronlherault} 11, 22 -- MIME-version: 1.0 11, 22 -- Content-type: text/plain; charset=US-ASCII 11, 22 -- Content-transfer-encoding: 7bit 11, 22 -- X-Mailer: Microsoft Office Outlook 11 11, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 11, 22 -- In-reply-to: {200903021016.n22AGCEg022238-at-ns.microscopy.com} 11, 22 -- Thread-index: AcmbH+2jqeAsRfXoS5ydfaBGEa4W5wAPCVew ==============================End of - Headers==============================
yes, in fact if you keep in close contact with companies they may even let you know if they are having a "closing down/stock clearance" sale at which point it may be worth stocking up.
Malcolm
----- Original Message ----- X-from: "Markus F. Meyenhofer" {micro-at-superlink.net}
Not sure if this group can offer any help but thought I would try.
I am trying to locate information on a Beckman Continuous Particle Electrophoresis System for clay separations mentioned in "The American Mineralogist, VOL 54, MAY-JUNE, 1969" paper by James I. Drever at Princeton.
System is no longer made, but hoping someone may have seen one or have one in a storeroom somewhere. Would love to find a system or locate a manual or detail drawing of the electrophoresis cell.
Any help is much appreciated.
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 26 -- From rbeavers-at-mail.smu.edu Mon Mar 2 12:01:39 2009 7, 26 -- Received: from sxet1p2.systems.smu.edu (sxet1p2.systems.smu.edu [129.119.65.147]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n22I1dG9027491 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Mar 2009 12:01:39 -0600 7, 26 -- Received: from sxht1p1.systems.smu.edu (129.119.65.132) by 7, 26 -- sxet1p2.systems.smu.edu (129.119.65.147) with Microsoft SMTP Server (TLS) id 7, 26 -- 8.1.340.0; Mon, 2 Mar 2009 12:01:39 -0600 7, 26 -- Received: from SXMBXC.systems.smu.edu ([129.119.65.166]) by 7, 26 -- sxht1p1.systems.smu.edu ([129.119.65.132]) with mapi; Mon, 2 Mar 2009 7, 26 -- 12:01:32 -0600 7, 26 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 26 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 26 -- Date: Mon, 2 Mar 2009 12:01:30 -0600 7, 26 -- Subject: clay separations 7, 26 -- Thread-Topic: clay separations 7, 26 -- Thread-Index: AcmbYOo79ac06aNhRcuAmXY9k/TMLw== 7, 26 -- Message-ID: {7A6FE75608A3624E872147993C8B36BB20C1FDA497-at-SXMBXC.systems.smu.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="iso-8859-1" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n22I1dG9027491 ==============================End of - Headers==============================
Friday Guest Speakers "High Resolution Fluorescence Measurements of the Muscle Contractile Apparatus" Doug Root, Associate Professor, University of North Texas
"A Demonstration of 3D Tomography" Lee Pullan, Senior Applications Engineer, FEI Company, Portland, Oregon
"Polymer Microscopy" Gary M. Brown, Exxon Mobil, Baytown, Texas
"Introduction to EDS and its Application in the Research in Pierce's disease of Grapes and Citrus Chlorosis" Brebo Leite, ThermoFisher
All forms and hotel information available on our web site: http://www.texasmicroscopy.org/ under the Spring 2009 nav button.
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Hi I think I should clarify that we have a service contract & good support from FEI. My main concern is the integrated DX4 pc, which has been upgraded as far as possible. So far we have been able to buy parts from FEI but I am worried about future sourcing of spares for our system as parts are no longer made. It's good to know about second hand supplies from elsewhere though, thanks for all your replies.
Regards Nikki
Nikki
Are you saying that FEI will not sell you the parts?
regards,
Jim
} From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2 Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From: Nicola.Weston-at-nottingham.ac.uk } Reply-to: Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hello } We have a FEI XL30 ESEM-FEG dating back a few years now and are } beginning to encounter problems with sourcing spare parts. Just } wondering if anyone else out there is running an xl30 esem and if so are } you having similar problems now they are no longer made,or does everyone } have a Quanta these days? } } Thanks } Nikki Weston } School M3 } University of Nottingham
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==============================Original Headers============================== 18, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 04:40:08 2009 18, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23Ae7QU031210 18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 04:40:07 -0600 18, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 18, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 18, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 18, 32 -- id 1LeS1J-0007KK-Tf 18, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 10:38:42 +0000 18, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 18, 32 -- Tue, 3 Mar 2009 10:38:40 +0000 18, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 18, 32 -- Content-class: urn:content-classes:message 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Type: text/plain; 18, 32 -- charset="us-ascii" 18, 32 -- Subject: Esem users 18, 32 -- Date: Tue, 3 Mar 2009 10:38:40 -0000 18, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94F8D6C9-at-VUIEXCHA.ad.nottingham.ac.uk} 18, 32 -- X-MS-Has-Attach: 18, 32 -- X-MS-TNEF-Correlator: 18, 32 -- Thread-Topic: Esem users 18, 32 -- Thread-Index: Acmb7DeAmHqObOSyRAG455Fy1t7uVw== 18, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 18, 32 -- To: {microscopy-at-microscopy.com} 18, 32 -- X-OriginalArrivalTime: 03 Mar 2009 10:38:40.0598 (UTC) FILETIME=[37B53760:01C99BEC] 18, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 18, 32 -- X-UoN-MailScanner: Found to be clean 18, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 18, 32 -- X-Spam-Status: No 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23Ae7QU031210 ==============================End of - Headers==============================
Maybe a dumb question but can a cryo diamond knife be used to cut resin sections at room temperature?
TIA Nikki
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==============================Original Headers============================== 5, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 06:46:59 2009 5, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23Ckw5M017434 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 06:46:59 -0600 5, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 5, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 5, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 5, 32 -- id 1LeU1F-0000iJ-Vi 5, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 12:46:46 +0000 5, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 5, 32 -- Tue, 3 Mar 2009 12:46:40 +0000 5, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 5, 32 -- Content-class: urn:content-classes:message 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Type: text/plain; 5, 32 -- charset="us-ascii" 5, 32 -- Subject: Ultramicrotomy - diamond knife question 5, 32 -- Date: Tue, 3 Mar 2009 12:46:40 -0000 5, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94F8D81A-at-VUIEXCHA.ad.nottingham.ac.uk} 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- Thread-Topic: Ultramicrotomy - diamond knife question 5, 32 -- Thread-Index: Acmb/hkKpFV7u4onQ1WNLoqKQKQAbg== 5, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 5, 32 -- To: {microscopy-at-microscopy.com} 5, 32 -- X-OriginalArrivalTime: 03 Mar 2009 12:46:40.0853 (UTC) FILETIME=[197F2050:01C99BFE] 5, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 5, 32 -- X-UoN-MailScanner: Found to be clean 5, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 5, 32 -- X-Spam-Status: No 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23Ckw5M017434 ==============================End of - Headers==============================
It really depends upon which type of cryo knife you have. In the case of ours all the cryo knives would cut the resin, but you would need the 'wet' knife to keep the section wet.
At 07:58 AM 3/3/2009, Nicola.Weston-at-nottingham.ac.uk wrote:
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==============================Original Headers============================== 19, 27 -- From jd-at-laddresearch.com Tue Mar 3 07:57:25 2009 19, 27 -- Received: from bean.electric.net (bean.electric.net [72.35.23.29]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23DvOa3001134 19, 27 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 07:57:24 -0600 19, 27 -- Received: from 1LeV7Z-0000RK-Th by bean.electric.net with emc1-ok (Exim 4.69) 19, 27 -- (envelope-from {jd-at-laddresearch.com} ) 19, 27 -- id 1LeV7Z-0000Rq-Uz; Tue, 03 Mar 2009 05:57:21 -0800 19, 27 -- Received: by emcmailer; Tue, 03 Mar 2009 05:57:21 -0800 19, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 19, 27 -- by bean.electric.net with esmtps (TLSv1:AES256-SHA:256) 19, 27 -- (Exim 4.69) 19, 27 -- (envelope-from {jd-at-laddresearch.com} ) 19, 27 -- id 1LeV7Z-0000RK-Th; Tue, 03 Mar 2009 05:57:21 -0800 19, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 19, 27 -- Date: Tue, 03 Mar 2009 08:57:17 -0500 19, 27 -- To: Nicola.Weston-at-nottingham.ac.uk 19, 27 -- From: jd {jd-at-laddresearch.com} 19, 27 -- Subject: Re: [Microscopy] Ultramicrotomy - diamond knife question 19, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 19, 27 -- In-Reply-To: {200903031258.n23CwtSH026420-at-ns.microscopy.com} 19, 27 -- References: {200903031258.n23CwtSH026420-at-ns.microscopy.com} 19, 27 -- Mime-Version: 1.0 19, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 19, 27 -- X-Outbound-IP: 216.204.198.170 19, 27 -- X-Env-From: jd-at-laddresearch.com 19, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 19, 27 -- Message-Id: {E1LeV7Z-0000Rq-Uz-at-bean.electric.net} ==============================End of - Headers==============================
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Email: spb-at-mwrn.com Name: Susanne Brandom
Organization: MC Services
Title-Subject: [Filtered] Workshop on Immunogold Silver Staining
Question: The Aurion ImmunoGold Silver Staining Workshop
Aurion and Electron Microscopy Sciences will hold a workshop on Immunogold Silver Staining at Albert Einstein College of Medicine from May 18 to 21. The course will be taught by Mr. Peter van de Plas who has been with Aurion since 1991. He worked closely together with Dr. Leunissen in founding a firm basis for Aurion and contributed to the development of product. He has been invited to many international microscopy conferences and workshops and is especially experienced in providing hands-on training.
The course will cover:
The properties of gold particles and their protein conjugates Theories underlying immunogold labeling protocols. Silver enhancement of gold particles. Immunogold labeling on a variety of sample preparations for LM. Immunogold labeling for EM Pre-/post-embedding double immunogold labeling. Background minimization in immunogold labeling
Participants are encouraged to bring their own samples, that will be processed under expert guidance. For more information contact Stacie Kirsch at Electron Microscopy Sciences. Please email stacie-at-ems-secure.com or call 215-412-8402 for details.
I hope my answer does not come too late! I have asked the same question as you on the list some years ago and I got several answers. Here is the very straightforward protocol I finally used on my EPON sections of intestine and it worked, although the staining was not very intense. I didnt really spend much time to improve the method though.
- Periodic acid 5%: 30 min at 50°C - Schiff Reagent: 30 min at 50°C - Post-staining: Azur II mix (it is a 1:1 mix of methylene blue and Azur II, more stable than methylene alone): 20 min RT (section thickness: 300 nm)
Additionally, one person told me he stained the glycogen using reduced osmium. Another one gave me this reference: Shroeder et al. (1980) "An established routine method for differential staining of epoxy-embedded tissue sections" Microscopia Acta 83,111-116
Best of luck
Stéphane
----- Original Message ---- X-from: "abrun-at-hsc.unt.edu" {abrun-at-hsc.unt.edu} To: nizets2-at-yahoo.com Sent: Wednesday, February 25, 2009 4:36:56 AM
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Email: abrun-at-hsc.unt.edu Name: Anne-Marie Brun
Organization: UNT HSC at Fort Worth Texas 76107, USA
Title-Subject: [Filtered] Epon/PAS stain
Question: Has anyone ever stained for PAS on Epon sections before? If not on Epon then what type of plastic did you use to do a PAS stain? I know it is done on paraffin embedded material, but an investigator wants the PAS staining done on Epon sections. ----? Thanks for your help Anne-Marie
Here is the March 2009 Microscopy Today table of contents. We will close the subscription list for this issue on Thursday, March 5, 2009. Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Technical Editor =====================
A Colorful New Way to Look at the Nuclear Pore! Stephen W. Carmichael, Mayo Clinic
On the Sub-Nanometer Resolution of Scanning Electron and Helium Ion Microscopes András E. Vladár, Michael T. Postek, and Bin Ming Nat'l. Inst. of Standards and Technology, Gaithersburg, MD
A Versatile and Affordable Plunge Freezing Instrument for Preparing Frozen Hydrated Specimens for Cryo Transmission Electron Microscopy (CryoEM) Linda Melanson, Gatan, Inc., Pleasanton, CA
Atomic Layer Deposition and Vapor Deposited SAMS in a CrossBeam FIB-SEM Platform: A Path To Advanced Materials Synthesis E. L. Principe†, C. Hartfield‡, R. Kruger‡, A. Smith‡, R. Dubois*, K. Scammon**, B. Kempshall*** †Carl Zeiss SMT, Inc.‡ Omniprobe, Inc.*, Colonial Metals, Inc.**, University of Central Florida, NanoSpective***
Pushing the Envelope in Atomic Force Microscopy M. G. Heaton & J. P. Cleveland, Asylum Research, Santa Barbara, CA
Dip Pen Nanolithography: A Desktop Nanofabrication Approach Using High-Throughput Flexible Nanopatterning Jason Haaheim and Omkar A. Nafday, NanoInk Inc.
A Compact Field Emission SEM for Low Voltage Imaging Jim Rynne, Novelx, Inc., Lafayette, CA
TEM Sample Preparation: An Interdisciplinary Website J. Ayache1, L. Beaunier2, J. Boumendil3, G. Ehret4 and D. Laub5, 1.CNRS-UMR, Villejuif, France; 2.CNRS-UPR, Paris; 3.U.Claude Bernard-Lyon, Villeurbanne, France; 4 Institut Physique et Chimie des Matériaux de Strasbourg, France; 5.EPFL-CIME, Lausanne, Switzerland
Microparticles/Exosomes: Isolation and TEM Analysis Natalie Bauer,Jyoti Rai, Hairu Chen, Lillianne Harris, Lalita Shevde, Tim Moore, and Judy King, U. of South Alabama, Mobile, AL
New Ultra-Thin Pure Silicon Window Grids for Transmission Electron Microscopy Samples James Roussie, TEMwindows.com, Rochester, NY
Preparing Biological Samples for Analysis by High Vacuum Techniques S.G. Ostrowski, T.L. Paxon, L. Denault, KP. McEvoy, and V.S. Smentkowski, General Electric Global Research Ctr., Niskayuna, NY
Pioneers in Optics: Friedrich Johann Karl Becke and Anders Jöns Ångström Michael W. Davidson, National High Magnetic Field Laboratory, The Florida State University, Tallahassee, FL
Microscopy-101: Glass Knives vs. Diamond Knives Lou Ann Miller, Center for Microscopic Imaging, Veterinary Medicine, University of Illinois
Industry News
NetNotes CHEMICALS - acrolein storage CHEMICALS – uranyl acetate safety CHEMICALS- cacodylate safety SPECIMEN PREPARATION - sand SPECIMEN PREPARATION - etching resin SPECIMEN PREPARATION – plan-view TEM samples SPECIMEN PREPARATION – stopping points MICROTOMY - histo knife IMMUNOCYTOCHEMISTRY – colloidal gold IMMUNOCYTOCHEMISTRY – choice of secondary antibodies SEM – current state of the art for biological specimens SEM – cooling EDS – Beryllium and copper EDX – Sn and Pb ratios
Dear Abbe
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==============================Original Headers============================== 21, 17 -- From randerson20-at-tampabay.rr.com Tue Mar 3 12:30:16 2009 21, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23IUFoK030150 21, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Mar 2009 12:30:16 -0600 21, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 21, 17 -- by hrndva-omta06.mail.rr.com with ESMTP 21, 17 -- id {20090303183015.NIVB6596.hrndva-omta06.mail.rr.com-at-[127.0.0.1]} 21, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Mar 2009 18:30:15 +0000 21, 17 -- Message-ID: {49AD772F.3090203-at-tampabay.rr.com} 21, 17 -- Date: Tue, 03 Mar 2009 13:30:07 -0500 21, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 21, 17 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 21, 17 -- MIME-Version: 1.0 21, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 21, 17 -- Subject: Microscopy Today March 2009 Table of Contents 21, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: sksears-at-eps.mcgill.ca Name: Kelly
Organization: McGill University
Title-Subject: [Filtered] Flooring for Cryo-(S)TEM
Question: We are installing a new cryo-(S)TEM and I would appreciate any suggestions for an appropriate floor covering that is resistant to liquid nitrogen spills. Thanks.
Hi Kelly, We did polished concrete floors for our entire new facility. No maintenance (no sealers or waxes necessary) and it is chemical and LN2 resistant.
Looks like glass but not slippery. john
At 02:08 PM 3/3/2009, you wrote:
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==============================Original Headers============================== 7, 22 -- From donovan-at-uoregon.edu Tue Mar 3 16:34:43 2009 7, 22 -- Received: from smtp.uoregon.edu (mserv5.uoregon.edu [128.223.142.42]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23MYfes001641 7, 22 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 16:34:42 -0600 7, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 7, 22 -- (authenticated bits=0) 7, 22 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n23MYb9A008944 7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 7, 22 -- Tue, 3 Mar 2009 14:34:37 -0800 7, 22 -- Message-Id: {200903032234.n23MYb9A008944-at-smtp.uoregon.edu} 7, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 22 -- Date: Tue, 03 Mar 2009 14:34:33 -0800 7, 22 -- To: sksears-at-eps.mcgill.ca 7, 22 -- From: John Donovan {donovan-at-uoregon.edu} 7, 22 -- Subject: Re: [Microscopy] viaWWW: Flooring for Cryo-(S)TEM 7, 22 -- Cc: microscopy-at-microscopy.com 7, 22 -- In-Reply-To: {200903032208.n23M8jmf027271-at-ns.microscopy.com} 7, 22 -- References: {200903032208.n23M8jmf027271-at-ns.microscopy.com} 7, 22 -- Mime-Version: 1.0 7, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 22 -- X-Virus-Scanned: ClamAV 0.94.2/9065/Tue Mar 3 02:43:41 2009 on mserv5 7, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
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Email: neh_123-at-yahoo.com Name: Nihar
Organization: U of M
Title-Subject: [Filtered] Silver enhancement for LM
Question: We are considering using silver enhancement kit from Ted Pella to detect gold on LM sections. Does anyone have any experince using it for LM for animal tissues such as blood, liver etc. ? I am new to histology and would appreciate any help/protocols.
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Tue Mar 3 19:33:12 2009 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n241XBU9020011 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 19:33:11 -0600 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240800c5d38ac0f400-at-[206.69.208.22]} 6, 11 -- Date: Tue, 3 Mar 2009 19:33:11 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: neh_123-at-yahoo.com (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: Silver enhancement for LM 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
From rjcju1hjjqivflg59d80qdfi7jhbie49er-at-4ax.com Wed Mar 4 03:58:25 2009 Return-Path: {rjcju1hjjqivflg59d80qdfi7jhbie49er-at-4ax.com} Received: from google.com (bft66.neoplus.adsl.tpnet.pl [83.28.57.66]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n249wOpO014312 for {microscopylistserverarchive-at-microscopy.com} ; Wed, 4 Mar 2009 03:58:25 -0600 Received: from [83.142.77.191] (HELO google.com) by flatthrowajoe.org; Wed, 4 Mar 2009 10:56:08 +0100 Message-ID: {00000000AC76E92394327263} Reply-To: Julianne Spring {17306alysha.benito-at-gmail.com}
I am sure the Pella product is good but I have found gold enhancement far superior to silver enhancement for both LM and EM immunocytochemistry. I like the Nanoprobes kit. Tom
Thomas E. Phillips, Ph.D. Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall Biological Sciences University of Missouri Columbia, MO 65211-7400 573-882-4712 (voice) 573-882-0123 (fax)
-----Original Message----- X-from: neh_123-at-yahoo.com [mailto:neh_123-at-yahoo.com] Sent: Tuesday, March 03, 2009 7:34 PM To: Phillips, Thomas E.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both neh_123-at-yahoo.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: neh_123-at-yahoo.com Name: Nihar
Organization: U of M
Title-Subject: [Filtered] Silver enhancement for LM
Question: We are considering using silver enhancement kit from Ted Pella to detect gold on LM sections. Does anyone have any experince using it for LM for animal tissues such as blood, liver etc. ? I am new to histology and would appreciate any help/protocols.
Hi Nikki, And there you have it: the number one reason not to abandon a pre-PC-controlled SEM - the PC.
I'm still servicing 30+ year old SEMs (at least one approaching 40 years) that still do yeoman's service day after day and year after year. If your SEM has the capabilities you need, hang on to it.
I'm sorry that I don't even have any suggestions on what to do when the vendor runs out of DX4 spares. Perhaps get a boat so that you can moor it to a very expensive anchor.
If the manufacturers would open their source code, someone who knows programming might be able to update the software to a newer PC as long as the connection is a network connection rather than proprietary driver boards for an obsolete bus.
It is up to the users to demand a longer view towards these 6 (and 7) figure investments.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
DISCLAIMER: Quality Images provides both per call and full service contracts on non-PC-controlled SEMs in the northeastern United States.
Sent: Tuesday, March 03, 2009 5:43 AM To: kenconverse-at-qualityimages.biz
Hi I think I should clarify that we have a service contract & good support from FEI. My main concern is the integrated DX4 pc, which has been upgraded as far as possible. So far we have been able to buy parts from FEI but I am worried about future sourcing of spares for our system as parts are no longer made. It's good to know about second hand supplies from elsewhere though, thanks for all your replies.
Regards Nikki
Nikki
Are you saying that FEI will not sell you the parts?
regards,
Jim
} From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2 Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From: Nicola.Weston-at-nottingham.ac.uk } Reply-to: Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hello } We have a FEI XL30 ESEM-FEG dating back a few years now and are } beginning to encounter problems with sourcing spare parts. Just } wondering if anyone else out there is running an xl30 esem and if so are } you having similar problems now they are no longer made,or does everyone } have a Quanta these days? } } Thanks } Nikki Weston } School M3 } University of Nottingham
This message has been checked for viruses but the contents of an attachment may still contain software viruses, which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 18, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 04:40:08 2009 18, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23Ae7QU031210 18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 04:40:07 -0600 18, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 18, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 18, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 18, 32 -- id 1LeS1J-0007KK-Tf 18, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 10:38:42 +0000 18, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 18, 32 -- Tue, 3 Mar 2009 10:38:40 +0000 18, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 18, 32 -- Content-class: urn:content-classes:message 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Type: text/plain; 18, 32 -- charset="us-ascii" 18, 32 -- Subject: Esem users 18, 32 -- Date: Tue, 3 Mar 2009 10:38:40 -0000 18, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94F8D6C9-at-VUIEXCHA.ad.nottingham.ac.uk} 18, 32 -- X-MS-Has-Attach: 18, 32 -- X-MS-TNEF-Correlator: 18, 32 -- Thread-Topic: Esem users 18, 32 -- Thread-Index: Acmb7DeAmHqObOSyRAG455Fy1t7uVw== 18, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 18, 32 -- To: {microscopy-at-microscopy.com} 18, 32 -- X-OriginalArrivalTime: 03 Mar 2009 10:38:40.0598 (UTC) FILETIME=[37B53760:01C99BEC] 18, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 18, 32 -- X-UoN-MailScanner: Found to be clean 18, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 18, 32 -- X-Spam-Status: No 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23Ae7QU031210 ==============================End of - Headers==============================
==============================Original Headers============================== 36, 26 -- From kenconverse-at-qualityimages.biz Wed Mar 4 09:09:21 2009 36, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24F9Kcg008063 36, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 09:09:20 -0600 36, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com 36, 26 -- with ESMTP 36, 26 -- id {20090304150920.KQDP12334.cdptpa-omta03.mail.rr.com-at-Ken} ; 36, 26 -- Wed, 4 Mar 2009 15:09:20 +0000 36, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 36, 26 -- To: {Nicola.Weston-at-nottingham.ac.uk} , 36, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 36, 26 -- Subject: RE: [Microscopy] Esem users 36, 26 -- Date: Wed, 4 Mar 2009 10:09:13 -0500 36, 26 -- Message-ID: {4A77CC536B84427DAE155946EF1CD301-at-Ken} 36, 26 -- MIME-Version: 1.0 36, 26 -- Content-Type: text/plain; 36, 26 -- charset="us-ascii" 36, 26 -- X-Priority: 3 (Normal) 36, 26 -- X-MSMail-Priority: Normal 36, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 36, 26 -- Importance: Normal 36, 26 -- Thread-Index: Acmb7OTN0pOE7cqsTp64DQ8z7DqyEgA6/3Eg 36, 26 -- In-Reply-To: {200903031043.n23AhSTB002816-at-ns.microscopy.com} 36, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 36, 26 -- Content-Transfer-Encoding: 8bit 36, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24F9Kcg008063 ==============================End of - Headers==============================
Nikki, how embedded is that PC? How peculiar is it?
I would think if you have a decent computer person around, you could upgrade on your own. There could be timing issues and you might need support for an ISA slot or two, but you should be able to do a lot. I would not count on the manufacturer for help.
We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 to a 1800 MHz AMD running Windows 2000. The manufacturer cautioned us along the way and would not guarantee that our upgrade would work. They had not tested our exact hardware and could not guarantee success. But neither did they promise failure. The upgrade worked fine. We do know that the software breaks when we upgrade to Windows XP, so we are maxed out with the OS if not the hardware. Fortunately, the hardware is current and stable and snappy as it is.
If you do need a replacement DX4, they are still out there on the market. I found several links to ones. I might even have one gathering dust at home. I know I have several older, working PCs with ISA slots.
Warren S.
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Wednesday, March 04, 2009 9:10 AM To: wesaia-at-iastate.edu
I'll second Warren's comments. I recently had an older, unsupported instrument whose computer died. I was able put together a system that revived the instrument. As he said, one big problem was ISA slot requirements (I needed 2!). Also like Warren, my ultimate limiting factor was that drivers wouldn't work with anything newer than Win2000, but that was definitely better the WinNT I started with! I ended up with a 3GHz Pentium 4 and plenty of RAM running Win2000, all in a PC with 2 ISA slots.
Boards with ISA slots are increasingly rare, but there are some vendors that concentrate on industrial applications. They have limited support for ISA slots, but the down side is their parts are typically expensive compared to off-the-shelf consumer items. Anyone can email me if they want the most economical source I found.
So It may not be easy, but you likely have some possibilities.
Jim
---------------------------------------------- Jim Passmore Research Associate Sealed Air Corporation james.passmore-at-sealedair.com ----------------------------------------------
wesaia-at-iastate.edu wrote on 03/04/2009 10:29:55 AM:
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} } Nikki, how embedded is that PC? How peculiar is it? } } I would think if you have a decent computer person around, you could } upgrade on your own. There could be timing issues and you might need } support for an ISA slot or two, but you should be able to do a lot. I } would not count on the manufacturer for help. } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 to a } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us along } the way and would not guarantee that our upgrade would work. They had } not tested our exact hardware and could not guarantee success. But } neither did they promise failure. The upgrade worked fine. We do know } that the software breaks when we upgrade to Windows XP, so we are maxed } out with the OS if not the hardware. Fortunately, the hardware is } current and stable and snappy as it is. } } If you do need a replacement DX4, they are still out there on the } market. I found several links to ones. I might even have one gathering } dust at home. I know I have several older, working PCs with ISA slots. } } Warren S. } }
==============================Original Headers============================== 13, 15 -- From James.Passmore-at-sealedair.com Wed Mar 4 11:14:09 2009 13, 15 -- Received: from dunnotesgw01.sealedair.com (dunnotesgw01.sealedair.com [165.225.194.17]) 13, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24HE89m007005 13, 15 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:14:08 -0600 13, 15 -- In-Reply-To: {200903041529.n24FTt66025164-at-ns.microscopy.com} 13, 15 -- Subject: Re: [Microscopy] Esem users 13, 15 -- To: Microscopy-at-microscopy.com 13, 15 -- X-Mailer: Lotus Notes Release 7.0.2 September 26, 2006 13, 15 -- Message-ID: {OF2BBE5F7B.9110BFAD-ON8525756F.005BF519-8525756F.005EAD1B-at-sealedair.com} 13, 15 -- From: James.Passmore-at-sealedair.com 13, 15 -- Date: Wed, 4 Mar 2009 12:08:56 -0500 13, 15 -- X-MIMETrack: Serialize by Router on DUNNOTESGW01/SAC(Release 7.0.2|September 26, 2006) at 13, 15 -- 03/04/2009 12:14:14 PM 13, 15 -- MIME-Version: 1.0 13, 15 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Just an echo of Ken's thoughts here. I guess I'm starting to qualify as an "old timer" at this point, but, at the risk of sounding like a Luddite, there is much to be said for an older style of non- or less-computer dominated microscope operations.
The rush to make everything controllable by computers through GUI's looks sexy on the monitors and can often seem to make things run more smoothly, but I can tell you from experience that some of the most reliable and fastest operating scopes were pre-computer-frenzy day machines (I think they're called "tools" now). Think Hitachi S570, which is fast and reliable and has accurate autofocus and contrast/brightness functions that are faster than I could adjust them by hand.
Sometimes I can believe that making everything run through a computer has less to do with efficiency and reliability than with being forced to appear to be as "advanced" as the competition. Do you really need a mouse and software to desaturate a filament/tip to center the halo?
I have heard from service people that it's often the computers that make their lives miserable. I also know that our most computer-dependent instruments are our most maintenance intensive, often involving time-consuming software reinstalls and problems with incompatibilities with other programs, networking issues, etc. Plus, tracking down a weird problem can be diabolically difficult if it's hard to tell if it's caused by software, computer hardware, or microscope system hardware. Our service support folks are sometimes nothing short of superhuman when it comes to their dedication and ingenuity at tracking down and fixing problems, but I bet sometimes it's a lot harder than it would have to be.
There is definitely a place for computerization of parts of microscope operation. Preset tip warm-up times are very nice in multi-user facilities like ours, for example. Motorized stage limits set by software for various types of holders are great. Automated tomography packages are super.
But is it really an all or nothing proposition when it comes to computer controlled operations? Maybe so. Not being an engineer, I'm not sure.
My two shares of AIG.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Wednesday, March 04, 2009 9:11 AM To: Tindall, Randy D.
Hi Nikki, And there you have it: the number one reason not to abandon a pre-PC-controlled SEM - the PC.
I'm still servicing 30+ year old SEMs (at least one approaching 40 years) that still do yeoman's service day after day and year after year. If your SEM has the capabilities you need, hang on to it.
I'm sorry that I don't even have any suggestions on what to do when the vendor runs out of DX4 spares. Perhaps get a boat so that you can moor it to a very expensive anchor.
If the manufacturers would open their source code, someone who knows programming might be able to update the software to a newer PC as long as the connection is a network connection rather than proprietary driver boards for an obsolete bus.
It is up to the users to demand a longer view towards these 6 (and 7) figure investments.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
DISCLAIMER: Quality Images provides both per call and full service contracts on non-PC-controlled SEMs in the northeastern United States.
Sent: Tuesday, March 03, 2009 5:43 AM To: kenconverse-at-qualityimages.biz
Hi I think I should clarify that we have a service contract & good support from FEI. My main concern is the integrated DX4 pc, which has been upgraded as far as possible. So far we have been able to buy parts from FEI but I am worried about future sourcing of spares for our system as parts are no longer made. It's good to know about second hand supplies from elsewhere though, thanks for all your replies.
Regards Nikki
Nikki
Are you saying that FEI will not sell you the parts?
regards,
Jim
} From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2 Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From: Nicola.Weston-at-nottingham.ac.uk } Reply-to: Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Hello } We have a FEI XL30 ESEM-FEG dating back a few years now and are } beginning to encounter problems with sourcing spare parts. Just } wondering if anyone else out there is running an xl30 esem and if so are } you having similar problems now they are no longer made,or does everyone } have a Quanta these days? } } Thanks } Nikki Weston } School M3 } University of Nottingham
This message has been checked for viruses but the contents of an attachment may still contain software viruses, which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 18, 32 -- From Nicola.Weston-at-nottingham.ac.uk Tue Mar 3 04:40:08 2009 18, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n23Ae7QU031210 18, 32 -- for {microscopy-at-microscopy.com} ; Tue, 3 Mar 2009 04:40:07 -0600 18, 32 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 18, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 18, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 18, 32 -- id 1LeS1J-0007KK-Tf 18, 32 -- for microscopy-at-microscopy.com; Tue, 03 Mar 2009 10:38:42 +0000 18, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 18, 32 -- Tue, 3 Mar 2009 10:38:40 +0000 18, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 18, 32 -- Content-class: urn:content-classes:message 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Type: text/plain; 18, 32 -- charset="us-ascii" 18, 32 -- Subject: Esem users 18, 32 -- Date: Tue, 3 Mar 2009 10:38:40 -0000 18, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94F8D6C9-at-VUIEXCHA.ad.nottingham.ac.uk} 18, 32 -- X-MS-Has-Attach: 18, 32 -- X-MS-TNEF-Correlator: 18, 32 -- Thread-Topic: Esem users 18, 32 -- Thread-Index: Acmb7DeAmHqObOSyRAG455Fy1t7uVw== 18, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 18, 32 -- To: {microscopy-at-microscopy.com} 18, 32 -- X-OriginalArrivalTime: 03 Mar 2009 10:38:40.0598 (UTC) FILETIME=[37B53760:01C99BEC] 18, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 18, 32 -- X-UoN-MailScanner: Found to be clean 18, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 18, 32 -- X-Spam-Status: No 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n23Ae7QU031210 ==============================End of - Headers==============================
==============================Original Headers============================== 36, 26 -- From kenconverse-at-qualityimages.biz Wed Mar 4 09:09:21 2009 36, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 36, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24F9Kcg008063 36, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 09:09:20 -0600 36, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com 36, 26 -- with ESMTP 36, 26 -- id {20090304150920.KQDP12334.cdptpa-omta03.mail.rr.com-at-Ken} ; 36, 26 -- Wed, 4 Mar 2009 15:09:20 +0000 36, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 36, 26 -- To: {Nicola.Weston-at-nottingham.ac.uk} , 36, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 36, 26 -- Subject: RE: [Microscopy] Esem users 36, 26 -- Date: Wed, 4 Mar 2009 10:09:13 -0500 36, 26 -- Message-ID: {4A77CC536B84427DAE155946EF1CD301-at-Ken} 36, 26 -- MIME-Version: 1.0 36, 26 -- Content-Type: text/plain; 36, 26 -- charset="us-ascii" 36, 26 -- X-Priority: 3 (Normal) 36, 26 -- X-MSMail-Priority: Normal 36, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 36, 26 -- Importance: Normal 36, 26 -- Thread-Index: Acmb7OTN0pOE7cqsTp64DQ8z7DqyEgA6/3Eg 36, 26 -- In-Reply-To: {200903031043.n23AhSTB002816-at-ns.microscopy.com} 36, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 36, 26 -- Content-Transfer-Encoding: 8bit 36, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24F9Kcg008063 ==============================End of - Headers==============================
==============================Original Headers============================== 53, 30 -- From TindallR-at-missouri.edu Wed Mar 4 12:01:49 2009 53, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 53, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24I1mE2021972 53, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:01:48 -0600 53, 30 -- X-IronPort-Anti-Spam-Filtered: true 53, 30 -- X-IronPort-Anti-Spam-Result: ApoEALVQrknRauUo/2dsb2JhbADIUAEJhxyITIJJDoExBg 53, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 53, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 04 Mar 2009 12:01:47 -0600 53, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 53, 30 -- Wed, 4 Mar 2009 12:01:48 -0600 53, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 53, 30 -- Content-class: urn:content-classes:message 53, 30 -- MIME-Version: 1.0 53, 30 -- Content-Type: text/plain; 53, 30 -- charset="us-ascii" 53, 30 -- Subject: RE: [Microscopy] RE: Esem users 53, 30 -- Date: Wed, 4 Mar 2009 12:00:54 -0600 53, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7EB4-at-UM-XMAIL08.um.umsystem.edu} 53, 30 -- In-Reply-To: {200903041510.n24FAjHL009692-at-ns.microscopy.com} 53, 30 -- X-MS-Has-Attach: 53, 30 -- X-MS-TNEF-Correlator: 53, 30 -- Thread-Topic: [Microscopy] RE: Esem users 53, 30 -- Thread-Index: Acmc22XkIZRLzLkQRy+gQeDoJDUDiwADWYvA 53, 30 -- References: {200903041510.n24FAjHL009692-at-ns.microscopy.com} 53, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 53, 30 -- To: {kenconverse-at-qualityimages.biz} 53, 30 -- Cc: {microscopy-at-microscopy.com} 53, 30 -- X-OriginalArrivalTime: 04 Mar 2009 18:01:48.0171 (UTC) FILETIME=[498CA5B0:01C99CF3] 53, 30 -- Content-Transfer-Encoding: 8bit 53, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24I1mE2021972 ==============================End of - Headers==============================
Allow me to chime in with a "hear hear, bravo". I agree completely. And anytime I've asked company reps why they're so insistent on shoving computerized everything down our throats, they always say "It's what our customers want." Except I've never heard a customer say they want everything computerized, needed or helpful or not. Nor have I ever understood the advantage to having a mouse (or other control) tell a GUI to tell a computer OS to tell a circuit to tell another circuit to reduce the (e.g.) objective lens by X amount when I could do as easily by turning a knob to adjust the lens-control circuit. With less chance of programming glitches, bugs, and all the extra failure modes. Maybe we should start a Luddites microscopy club.
Phil
} Just an echo of Ken's thoughts here. I guess I'm starting to qualify as } an "old timer" at this point, but, at the risk of sounding like a } Luddite, there is much to be said for an older style of non- or } less-computer dominated microscope operations. } } The rush to make everything controllable by computers through GUI's } looks sexy on the monitors and can often seem to make things run more } smoothly, but I can tell you from experience that some of the most } reliable and fastest operating scopes were pre-computer-frenzy day } machines (I think they're called "tools" now). Think Hitachi S570, } which is fast and reliable and has accurate autofocus and } contrast/brightness functions that are faster than I could adjust them } by hand. } } Sometimes I can believe that making everything run through a computer } has less to do with efficiency and reliability than with being forced to } appear to be as "advanced" as the competition. Do you really need a } mouse and software to desaturate a filament/tip to center the halo? } } I have heard from service people that it's often the computers that make } their lives miserable. I also know that our most computer-dependent } instruments are our most maintenance intensive, often involving } time-consuming software reinstalls and problems with incompatibilities } with other programs, networking issues, etc. Plus, tracking down a } weird problem can be diabolically difficult if it's hard to tell if it's } caused by software, computer hardware, or microscope system hardware. } Our service support folks are sometimes nothing short of superhuman when } it comes to their dedication and ingenuity at tracking down and fixing } problems, but I bet sometimes it's a lot harder than it would have to } be. } } There is definitely a place for computerization of parts of microscope } operation. Preset tip warm-up times are very nice in multi-user } facilities like ours, for example. Motorized stage limits set by } software for various types of holders are great. Automated tomography } packages are super. } } But is it really an all or nothing proposition when it comes to computer } controlled operations? Maybe so. Not being an engineer, I'm not sure. } } My two shares of AIG. } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz } [mailto:kenconverse-at-qualityimages.biz] } Sent: Wednesday, March 04, 2009 9:11 AM } To: Tindall, Randy D. } Subject: [Microscopy] RE: Esem users } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 25 -- From oshel1pe-at-cmich.edu Wed Mar 4 12:20:40 2009 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24IKeG8003638 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:20:40 -0600 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n24IKVv3024268 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:20:38 -0500 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 25 -- Wed, 4 Mar 2009 13:20:38 -0500 4, 25 -- Mime-Version: 1.0 4, 25 -- Message-Id: {f06240808c5d4755f7327-at-[141.209.160.249]} 4, 25 -- In-Reply-To: {200903041804.n24I4kLp027816-at-ns.microscopy.com} 4, 25 -- References: {200903041804.n24I4kLp027816-at-ns.microscopy.com} 4, 25 -- Date: Wed, 4 Mar 2009 13:20:36 -0500 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 25 -- Subject: Re: [Microscopy] Esem users 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 25 -- X-OriginalArrivalTime: 04 Mar 2009 18:20:38.0144 (UTC) FILETIME=[EB10D800:01C99CF5] 4, 25 -- X-Canit-CHI2: 0.00 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 25 -- X-Spam-Score: -3.40 () [Hold at 5.00] L_EXCH_MF,L_USD,PORN_RP_SEXY,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5) 4, 25 -- X-CanItPRO-Stream: default 4, 25 -- X-Canit-Stats-ID: 9837315 - bc2a61c2f0ad 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Ooooh, count me in as a Luddite, then. We just replaced our Hitachi S-800 FESEM (a GREAT tool) with a Hitachi S-4800 FESEM (another GREAT tool). All the youngins were excited about the coming computerization, and most said things like "Oh, it will be easier and faster, now", and then were astonished to find it somewhat less intuitive and a LOT slower to use. And I can't believe how long it takes to boot!
Don't get me wrong; this instrument has five detectors and we need the computer to mix 'em and get digital images and all, but I find everyone using the few supplied knobs to focus, adjust brightness and contrast, stigmate, and change mag in spite of having those functions available by mouse and scroll bars, because it's faster. But it would sure be nice to be able to use our microscopes even if the software got all unstable on us!
I'd love to shut off the computer to my TEM and just let the beam shoot straight down the column! It's then a pretty simple device.
Aloha from rainy but warm Honolulu, Tina
} Allow me to chime in with a "hear hear, bravo". } I agree completely. And anytime I've asked company reps why they're } so insistent on shoving computerized everything down our throats, } they always say "It's what our customers want." Except I've never } heard a customer say they want everything computerized, needed or } helpful or not. } Nor have I ever understood the advantage to having a mouse (or other } control) tell a GUI to tell a computer OS to tell a circuit to tell } another circuit to reduce the (e.g.) objective lens by X amount when } I could do as easily by turning a knob to adjust the lens-control } circuit. With less chance of programming glitches, bugs, and all the } extra failure modes. } Maybe we should start a Luddites microscopy club. } } Phil } } } Just an echo of Ken's thoughts here. I guess I'm starting to qualify as } } an "old timer" at this point, but, at the risk of sounding like a } } Luddite, there is much to be said for an older style of non- or } } less-computer dominated microscope operations. } } } } The rush to make everything controllable by computers through GUI's } } looks sexy on the monitors and can often seem to make things run more } } smoothly, but I can tell you from experience that some of the most } } reliable and fastest operating scopes were pre-computer-frenzy day } } machines (I think they're called "tools" now). Think Hitachi S570, } } which is fast and reliable and has accurate autofocus and } } contrast/brightness functions that are faster than I could adjust them } } by hand. } } } } Sometimes I can believe that making everything run through a computer } } has less to do with efficiency and reliability than with being forced to } } appear to be as "advanced" as the competition. Do you really need a } } mouse and software to desaturate a filament/tip to center the halo? } } } } I have heard from service people that it's often the computers that make } } their lives miserable. I also know that our most computer-dependent } } instruments are our most maintenance intensive, often involving } } time-consuming software reinstalls and problems with incompatibilities } } with other programs, networking issues, etc. Plus, tracking down a } } weird problem can be diabolically difficult if it's hard to tell if it's } } caused by software, computer hardware, or microscope system hardware. } } Our service support folks are sometimes nothing short of superhuman when } } it comes to their dedication and ingenuity at tracking down and fixing } } problems, but I bet sometimes it's a lot harder than it would have to } } be. } } } } There is definitely a place for computerization of parts of microscope } } operation. Preset tip warm-up times are very nice in multi-user } } facilities like ours, for example. Motorized stage limits set by } } software for various types of holders are great. Automated tomography } } packages are super. } } } } But is it really an all or nothing proposition when it comes to computer } } controlled operations? Maybe so. Not being an engineer, I'm not sure. } } } } My two shares of AIG. } } } } Cheers, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } Week&NavType=Both&Type=TimePlan } } Sons of Norway: http://www.sofn.com } } } } } } } } -----Original Message----- } } X-from: kenconverse-at-qualityimages.biz } } [mailto:kenconverse-at-qualityimages.biz] } } Sent: Wednesday, March 04, 2009 9:11 AM } } To: Tindall, Randy D. } } Subject: [Microscopy] RE: Esem users } } } } } } } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ } } ---- } } } } Hi Nikki, } } And there you have it: the number one reason not to abandon a } } pre-PC-controlled SEM - the PC. } } } } I'm still servicing 30+ year old SEMs (at least one approaching 40 } } years) } } that still do yeoman's service day after day and year after year. If } } your } } SEM has the capabilities you need, hang on to it. } } } } I'm sorry that I don't even have any suggestions on what to do when the } } vendor runs out of DX4 spares. Perhaps get a boat so that you can moor } } it } } to a very expensive anchor. } } } } If the manufacturers would open their source code, someone who knows } } programming might be able to update the software to a newer PC as long } } as } } the connection is a network connection rather than proprietary driver } } boards } } for an obsolete bus. } } } } It is up to the users to demand a longer view towards these 6 (and 7) } } figure } } investments. } } } } Ken Converse } } owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } DISCLAIMER: Quality Images provides both per call and full service } } contracts on non-PC-controlled SEMs in the northeastern United States. } } } } } } -----Original Message----- } } X-from: Nicola.Weston-at-nottingham.ac.uk } } [mailto:Nicola.Weston-at-nottingham.ac.uk] } } } } Sent: Tuesday, March 03, 2009 5:43 AM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] Esem users } } } } } } } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ } } ---- } } } } } } Hi } } I think I should clarify that we have a service contract & good support } } from FEI. My main concern is the integrated DX4 pc, which has been } } upgraded as far as possible. So far we have been able to buy parts from } } FEI but I am worried about future sourcing of spares for our system as } } parts are no longer made. } } It's good to know about second hand supplies from elsewhere though, } } thanks for all your replies. } } } } Regards } } Nikki } } } } } } } } } } } } } } } } Nikki } } } } Are you saying that FEI will not sell you the parts? } } } } regards, } } } } Jim } } } } } } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: Mon, 2 } } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } From: } } Nicola.Weston-at-nottingham.ac.uk } Reply-to: } } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy Listserver" } } {microscopy-at-microscopy.com} } Subject: [Microscopy] ESEM users } } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } } } } } } } } } } } ------------------------------------------------------------------------ } } ---- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ------------------------------------------------------------------------ } } ---- } } } } } } Hello } } } We have a FEI XL30 ESEM-FEG dating back a few years now and are } } } beginning to encounter problems with sourcing spare parts. Just } } } wondering if anyone else out there is running an xl30 esem and if so are } } } you having similar problems now they are no longer made,or does } } everyone } have a Quanta these days? } } } } } } Thanks } } } Nikki Weston } } } School M3 } } } University of Nottingham } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } ==============================Original Headers============================== } 4, 25 -- From oshel1pe-at-cmich.edu Wed Mar 4 12:20:40 2009 } 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24IKeG8003638 } 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:20:40 -0600 } 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n24IKVv3024268 } 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:20:38 -0500 } 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); } 4, 25 -- Wed, 4 Mar 2009 13:20:38 -0500 } 4, 25 -- Mime-Version: 1.0 } 4, 25 -- Message-Id: {f06240808c5d4755f7327-at-[141.209.160.249]} } 4, 25 -- In-Reply-To: {200903041804.n24I4kLp027816-at-ns.microscopy.com} } 4, 25 -- References: {200903041804.n24I4kLp027816-at-ns.microscopy.com} } 4, 25 -- Date: Wed, 4 Mar 2009 13:20:36 -0500 } 4, 25 -- To: Microscopy-at-microscopy.com } 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 4, 25 -- Subject: Re: [Microscopy] Esem users } 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 4, 25 -- X-OriginalArrivalTime: 04 Mar 2009 18:20:38.0144 (UTC) FILETIME=[EB10D800:01C99CF5] } 4, 25 -- X-Canit-CHI2: 0.00 } 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 4, 25 -- X-Spam-Score: -3.40 () [Hold at 5.00] L_EXCH_MF,L_USD,PORN_RP_SEXY,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5) } 4, 25 -- X-CanItPRO-Stream: default } 4, 25 -- X-Canit-Stats-ID: 9837315 - bc2a61c2f0ad } 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Wed Mar 4 12:35:51 2009 7, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24IZoY1017543 7, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:35:51 -0600 7, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n24IZgQf010366 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 21 -- Wed, 4 Mar 2009 08:35:43 -1000 (HST) 7, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n24IZft6010363; 7, 21 -- Wed, 4 Mar 2009 08:35:41 -1000 (HST) 7, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Wed, 4 Mar 2009 08:35:40 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-Sender: tina-at-halia 7, 21 -- To: oshel1pe-at-cmich.edu 7, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] Re: Esem users 7, 21 -- In-Reply-To: {200903041821.n24ILQqa005092-at-ns.microscopy.com} 7, 21 -- Message-ID: {Pine.GSO.4.21.0903040826180.10296-100000-at-halia} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Maybe I'm just a computer geek or maybe it's the FEI interface (which I find it simple, easy and intuitive), but even with the dedicated knob set (which I insisted on when we bought the instrument, having been an "analog knob feel" junkie myself), I find I only ever use the big mag knob to zoom in or out.
The mouse based focus and stig I find effortless to use and very responsive (it's right button click and drag (in X) to focus and shift right click and drag (in X and Y) to stigmate).
I am completely won over by the FEI Quanta ESEM computer control much to my surprise. john
At 10:40 AM 3/4/2009, tina-at-pbrc.hawaii.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 21 -- From donovan-at-uoregon.edu Wed Mar 4 13:04:01 2009 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24J40vi031984 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:04:00 -0600 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 8, 21 -- (authenticated bits=0) 8, 21 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n24J3x0h006753 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:04:00 -0800 8, 21 -- Message-Id: {200903041904.n24J3x0h006753-at-smtp.uoregon.edu} 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Wed, 04 Mar 2009 11:03:49 -0800 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} 8, 21 -- Subject: Re: [Microscopy] Esem users 8, 21 -- In-Reply-To: {200903041840.n24IewXj025217-at-ns.microscopy.com} 8, 21 -- References: {200903041840.n24IewXj025217-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 21 -- X-Virus-Scanned: ClamAV 0.94.2/9067/Wed Mar 4 02:06:09 2009 on mserv1 8, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Personally, I've had experience working with high-end computing equipment (Not necessarily microscopy related) and I know how sensitive it can get. I just discoverd that my analog SEM developed a short in the power transformer and was delivering 130V AC to the instrument instead of the required 100V. The over-voltage would just about kill any modern digital apparatus I could think of, but my scope kept working until a couple of resistors on a scan amp board fried. That's when I discovered the problem. Transformer replaced, resistors replaced, and I'm running again!
Let's see anyone do that with a modern digital instrument...
Justin A. Kraft
On Wed, Mar 4, 2009 at 12:19 PM, {James.Passmore-at-sealedair.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'll second Warren's comments. I recently had an older, unsupported } instrument whose computer died. I was able put together a system that } revived the instrument. As he said, one big problem was ISA slot } requirements (I needed 2!). Also like Warren, my ultimate limiting factor } was that drivers wouldn't work with anything newer than Win2000, but that } was definitely better the WinNT I started with! I ended up with a 3GHz } Pentium 4 and plenty of RAM running Win2000, all in a PC with 2 ISA slots. } } Boards with ISA slots are increasingly rare, but there are some vendors } that concentrate on industrial applications. They have limited support for } ISA slots, but the down side is their parts are typically expensive } compared to off-the-shelf consumer items. Anyone can email me if they want } the most economical source I found. } } So It may not be easy, but you likely have some possibilities. } } Jim } } ---------------------------------------------- } Jim Passmore } Research Associate } Sealed Air Corporation } james.passmore-at-sealedair.com } ---------------------------------------------- } } } } } wesaia-at-iastate.edu wrote on 03/04/2009 10:29:55 AM: } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } Nikki, how embedded is that PC? How peculiar is it? } } } } I would think if you have a decent computer person around, you could } } upgrade on your own. There could be timing issues and you might need } } support for an ISA slot or two, but you should be able to do a lot. I } } would not count on the manufacturer for help. } } } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 to a } } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us along } } the way and would not guarantee that our upgrade would work. They had } } not tested our exact hardware and could not guarantee success. But } } neither did they promise failure. The upgrade worked fine. We do know } } that the software breaks when we upgrade to Windows XP, so we are maxed } } out with the OS if not the hardware. Fortunately, the hardware is } } current and stable and snappy as it is. } } } } If you do need a replacement DX4, they are still out there on the } } market. I found several links to ones. I might even have one gathering } } dust at home. I know I have several older, working PCs with ISA slots. } } } } Warren S. } } } } } } } ==============================Original Headers============================== } 13, 15 -- From James.Passmore-at-sealedair.com Wed Mar 4 11:14:09 2009 } 13, 15 -- Received: from dunnotesgw01.sealedair.com (dunnotesgw01.sealedair.com [165.225.194.17]) } 13, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24HE89m007005 } 13, 15 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:14:08 -0600 } 13, 15 -- In-Reply-To: {200903041529.n24FTt66025164-at-ns.microscopy.com} } 13, 15 -- Subject: Re: [Microscopy] Esem users } 13, 15 -- To: Microscopy-at-microscopy.com } 13, 15 -- X-Mailer: Lotus Notes Release 7.0.2 September 26, 2006 } 13, 15 -- Message-ID: {OF2BBE5F7B.9110BFAD-ON8525756F.005BF519-8525756F.005EAD1B-at-sealedair.com} } 13, 15 -- From: James.Passmore-at-sealedair.com } 13, 15 -- Date: Wed, 4 Mar 2009 12:08:56 -0500 } 13, 15 -- X-MIMETrack: Serialize by Router on DUNNOTESGW01/SAC(Release 7.0.2|September 26, 2006) at } 13, 15 -- 03/04/2009 12:14:14 PM } 13, 15 -- MIME-Version: 1.0 } 13, 15 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers============================== }
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
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OK, I have to go along with everyone else on this one. I have a different take on the situation, so here goes.
After I learned how to align the TEM, I kind of forgot the names of all the controls. It became second nature to follow a routine, I knew where to reach for the knob I needed, I didn't have to remember its name or look to see what it was labeled. I could do it in the dark, almost in my sleep. It was easy. All the motor movements and kinesthetic learning things helped me 'know' where to reach and what to do without looking. Besides, in those days it was dark, I couldn't have read a label if I wanted to.
I recently had some experience with a 'modern' microscope. Computer and mouse did the controlling. First, the darn computer screen was too bright and made it hard to keep my eyes dark adapted. Second, since I forgot the real names of the knobs I needed to manipulate, just knew where to reach, I had a heck of a time relearning what to call them so I could click the mouse on the correct 'button' on the computer. As screens changed, the things I wanted were moving all over the place and it was harder to keep track of where the control I needed was to be found.
And here is the ultimate insult. With old age has come poor close vision. So now, even if I knew what I was looking for in the computer screen, I can't see it without my reading glasses. So, its glasses on to read the screen, glasses off to look through the binocs, glasses on to check something on the screen and back and forth. Very unhandy. In the old days, I could run the microscope controls by the braille method, no seeing needed, now it is not so handy.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 44 -- From jkrupp-at-deltacollege.edu Wed Mar 4 13:07:17 2009 9, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 9, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n24J7G9p006706 9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:07:16 -0600 9, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 9, 44 -- by localhost (Email Security Appliance) with SMTP id B1B8F213C5E_9AECBD2B 9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 18:43:30 +0000 (GMT) 9, 44 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 9, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id AF2411B6095_9AECB82F 9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 18:41:05 +0000 (GMT) 9, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 9, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 9, 44 -- with ESMTP id 45893833 for Microscopy-at-microscopy.com; Wed, 04 Mar 2009 11:03:37 -0800 9, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 9, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 9, 44 -- ESMTP id KFZW6T00.KRA for {Microscopy-at-microscopy.com} ; Wed, 4 9, 44 -- Mar 2009 10:47:18 -0800 9, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C27FF8F74E7A 9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:03:36 -0800 (PST) 9, 44 -- X-Virus-Scanned: amavisd-new at 9, 44 -- X-Spam-Flag: NO 9, 44 -- X-Spam-Score: -2.499 9, 44 -- X-Spam-Level: 9, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 9, 44 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1] 9, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 9, 44 -- with ESMTP id 0AfCIaoBDC6u for {Microscopy-at-microscopy.com} ; 9, 44 -- Wed, 4 Mar 2009 11:03:36 -0800 (PST) 9, 44 -- Received: from [172.20.3.10] (unknown [172.20.3.10]) 9, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 515438F74E76 9, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:03:36 -0800 (PST) 9, 44 -- Message-Id: {09F26248-15FB-472E-AFCD-4E4E1F66FB1C-at-deltacollege.edu} 9, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 9, 44 -- To: Microscopy-at-microscopy.com 9, 44 -- In-Reply-To: {200903041843.n24IhlBU030143-at-ns.microscopy.com} 9, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 44 -- Content-Transfer-Encoding: 7bit 9, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3) 9, 44 -- Subject: Re: [Microscopy] Esem users 9, 44 -- Date: Wed, 4 Mar 2009 11:03:35 -0800 9, 44 -- References: {200903041843.n24IhlBU030143-at-ns.microscopy.com} 9, 44 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I've enjoyed reading this thread, and thought I'd add something from a slightly different perspective. After 35 years using LOM for metallography, I recently inherited the job and the tool (an old Cambridge S200) after our x-ray guy of 25 years was downsized (not my decision). Having never used an SEM or contemplated x-ray microanalysis in my life, you can imagine I was a bit overwhelmed by a control panel, never mind the science, that would make a space-shuttle pilot cringe. I'm a little over a year into it now, and still dreamed of how wonderful it must be to use a state of the art, mouse controlled, LN2 free tool...until I began following this thread. I think I'll stick with ole Betsy. I've become comfortable with her eccentricities.
Jeff Stewart Metallographic Lab Manager Stern-Leach Company 49 Pearl Street Attleboro, MA 02703 508-222-7400 x1329 ----- Original Message ----- X-from: {tina-at-pbrc.hawaii.edu} To: {jeff-at-metallography.com} Sent: Wednesday, March 04, 2009 1:36 PM
Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean and only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty.
Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back.
My 2 cents Canadian (~1.56 cents US),
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
this does raise one or two interesting points about computers in microscopy.
We fairly easily upgraded the operating system on our Link EDS system several years ago but had a lot of problems even discussing the use of new printer drivers for our Hitachi S3000N.
I get the impression that microscope control systems and imaging are so heavily integrated into their computers that a simple upgrade is not possible because of the complexity of hardware, bespoke drivers, special software and the operating system and peripherals. Maybe this will change with 2nd and 3rd generation systems.
Analytical EDS systems which have been computerized for years seem a bit easier because they have less integrated controls, no active imaging and software packages seem a bit more portable.
Maybe as well as looking out for sales of microscopy spares it might be useful to keep a couple of legacy computers for the technologies that won't migrate onto newer computers.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: wesaia-at-iastate.edu
Yes, in response to Malcom.
Another example: one of our scopes still runs Window NT Workstation. To upgrade to an operating system from this century will cost from $20,000-40,000, depending on who I've talked to. Our IT people won't touch this system. Once, back in the days of ZIP drives, our drive failed and we couldn't get images off the scope. We couldn't get our LAN or CD writer to work, either. When we reinstalled the drivers for the drive, the scope went down. Took a $7000 service call to get it running again. I actually had to send a claim to our insurance people for that amount for "Installing ZIP drivers" (we had made an ill-advised experiment with insurance companies vs. service contracts---don't).
So, yes also to Jim---keep your controller computers squeaky clean. Play games and chat on your laptop.
Another, more recent, example: one scope had an intermittent alarm going off and when it went off half the controls would lock up. No beam brightness control and only half of the beam traverse and alignment controls would work (x-axis only). Literally weeks of trouble shooting went into diagnosing this, during which we could use the scope only part-time, when the alarm wasn't going off. Finally we realized it was the computer case speaker making the noise, not the scope. I finally figured out how to shut off the case speaker to save our sanity (Google is a wonderful thing), and when I did the scope quit malfunctioning. This one left everyone from Columbia, MO to Tokyo scratching their heads.
Our service engineer replaced a couple likely suspect components and now things are back to normal, but we still don't know for sure what malfunctioned. This is not a dig at the service people---ours are stellar. It just is an example of how complicated things can be.
So I'm back to my original point: computer-controlled functions are often really great things, as a couple posters have pointed out. But does it have to be for everything?
Randy
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Wednesday, March 04, 2009 1:27 PM To: Tindall, Randy D.
Warren
this does raise one or two interesting points about computers in microscopy.
We fairly easily upgraded the operating system on our Link EDS system several years ago but had a lot of problems even discussing the use of new printer drivers for our Hitachi S3000N.
I get the impression that microscope control systems and imaging are so heavily integrated into their computers that a simple upgrade is not possible because of the complexity of hardware, bespoke drivers, special software and the operating system and peripherals. Maybe this will change with 2nd and 3rd generation systems.
Analytical EDS systems which have been computerized for years seem a bit easier because they have less integrated controls, no active imaging and software packages seem a bit more portable.
Maybe as well as looking out for sales of microscopy spares it might be useful to keep a couple of legacy computers for the technologies that won't migrate onto newer computers.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: wesaia-at-iastate.edu
Let me add another perspective on mouse verses knobs: After 36 years of microscopy, I have never seen anyone injure their hands twiddling knobs. However, at Intel and now Numonyx, repetitive stress injuries, also known as musculo-skeletal injuries, or MSDs are epidemic. Tendon and nerve injuries from the shoulders to the fingers constitute the largest fraction of industrial injuries in the semiconductor industry. It may seem unbelievable that with all those big factories more people get injured pushing a mouse around and clicking than driving forklifts, cranes, wafer robots etc. put together. These injuries are not life threatening but are frequently career ending because they tend to take years to heal, and the individual is basically functionally disabled. I have experienced these injuries myself, so for me the next time I go shopping for a new whatever, it better be run by knobs.
John Mardinly, Numonyx
-----Original Message----- X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] Sent: Wednesday, March 04, 2009 11:14 AM To: MARDINLY, A
Hi all, Just an alternative perspective.
Maybe I'm just a computer geek or maybe it's the FEI interface (which I find it simple, easy and intuitive), but even with the dedicated knob set (which I insisted on when we bought the instrument, having been an "analog knob feel" junkie myself), I find I only ever use the big mag knob to zoom in or out.
The mouse based focus and stig I find effortless to use and very responsive (it's right button click and drag (in X) to focus and shift right click and drag (in X and Y) to stigmate).
I am completely won over by the FEI Quanta ESEM computer control much to my surprise. john
At 10:40 AM 3/4/2009, tina-at-pbrc.hawaii.edu wrote: } ----------------------------------------------------------------------- ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 21 -- From donovan-at-uoregon.edu Wed Mar 4 13:04:01 2009 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24J40vi031984 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:04:00 -0600 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 8, 21 -- (authenticated bits=0) 8, 21 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n24J3x0h006753 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:04:00 -0800 8, 21 -- Message-Id: {200903041904.n24J3x0h006753-at-smtp.uoregon.edu} 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Wed, 04 Mar 2009 11:03:49 -0800 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} 8, 21 -- Subject: Re: [Microscopy] Esem users 8, 21 -- In-Reply-To: {200903041840.n24IewXj025217-at-ns.microscopy.com} 8, 21 -- References: {200903041840.n24IewXj025217-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 21 -- X-Virus-Scanned: ClamAV 0.94.2/9067/Wed Mar 4 02:06:09 2009 on mserv1 8, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From A.MARDINLY-at-numonyx.com Wed Mar 4 14:02:21 2009 16, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24K2LVo031089 16, 29 -- for {Microscopy-at-Microscopy.com} ; Wed, 4 Mar 2009 14:02:21 -0600 16, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 16, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id F15C0144007; 16, 29 -- Wed, 4 Mar 2009 13:06:39 -0500 (EST) 16, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 16, 29 -- Wed, 4 Mar 2009 15:02:21 -0500 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 29 -- Content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="us-ascii" 16, 29 -- Subject: RE: [Microscopy] Re: Esem users 16, 29 -- Date: Wed, 4 Mar 2009 15:01:38 -0500 16, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9EE9490-at-EXDRESBENMX012.numonyx.local} 16, 29 -- In-Reply-To: {200903041914.n24JE3nw031358-at-ns.microscopy.com} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] Re: Esem users 16, 29 -- Thread-Index: Acmc/W1LTvDXd8D2Q7OtHKARYKs5ZQABVcug 16, 29 -- References: {200903041914.n24JE3nw031358-at-ns.microscopy.com} 16, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 16, 29 -- To: {donovan-at-uoregon.edu} 16, 29 -- Cc: {Microscopy-at-Microscopy.com} 16, 29 -- X-OriginalArrivalTime: 04 Mar 2009 20:02:21.0381 (UTC) FILETIME=[20E0E750:01C99D04] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24K2LVo031089 ==============================End of - Headers==============================
I recently purchased a SEM for my work and having pretty much carte blanc, I decided on a rather less followed path. I purchased an older column and control panel that was just pre-computer computer age having excellent optics. I then had a brand new analytical interface put on it.
This makes my base SEM rather easy to work on and keep running and a very nice interface for computer control that comes with free upgrades for the life of the system.
In this way, I can use whatever current computer operating system happens to exist, get computer control (somewhat limited) on my SEM with all components repairable and upgradable for as many years as I will ever work on this tool.
I also have to say that this list was key in me making this choice as I've been hearing for a long time people complaining about the embeded computer microchips having long term maintainence problems.
dj
On Wed, 4 Mar 2009, malcolm.haswell-at-sunderland.ac.uk wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Warren } } this does raise one or two interesting points about computers in } microscopy. } } We fairly easily upgraded the operating system on our Link EDS system } several years ago but had a lot of problems even discussing the use of } new printer drivers for our Hitachi S3000N. } } I get the impression that microscope control systems and imaging are } so heavily integrated into their computers that a simple upgrade is } not possible because of the complexity of hardware, bespoke drivers, } special software and the operating system and peripherals. Maybe this } will change with 2nd and 3rd generation systems. } } Analytical EDS systems which have been computerized for years seem a } bit easier because they have less integrated controls, no active } imaging and software packages seem a bit more portable. } } Maybe as well as looking out for sales of microscopy spares it might } be useful to keep a couple of legacy computers for the technologies } that won't migrate onto newer computers. } } Malcolm } } Malcolm Haswell } Electron Microscope Unit } Faculty of Applied Sciences } University of Sunderland } SUNDERLAND } SR1 3SD } UK } } email: malcolm.haswell-at-sunderland.ac.uk } } } ----- Original Message ----- } X-from: wesaia-at-iastate.edu } Date: Wednesday, March 4, 2009 3:33 pm } Subject: [Microscopy] Esem users } } } } } } } } } ------------------------------------------------------------------- } } --------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } AmericaTo Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserverOn-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------ } } ---------------------------------------------------------------- } } } } Nikki, how embedded is that PC? How peculiar is it? } } } } I would think if you have a decent computer person around, you could } } upgrade on your own. There could be timing issues and you might need } } support for an ISA slot or two, but you should be able to do a } } lot. I } } would not count on the manufacturer for help. } } } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 } } to a } } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us } along } } the way and would not guarantee that our upgrade would work. They had } } not tested our exact hardware and could not guarantee success. But } } neither did they promise failure. The upgrade worked fine. We do know } } that the software breaks when we upgrade to Windows XP, so we are } } maxedout with the OS if not the hardware. Fortunately, the } } hardware is } } current and stable and snappy as it is. } } } } If you do need a replacement DX4, they are still out there on the } } market. I found several links to ones. I might even have one } gathering } } dust at home. I know I have several older, working PCs with ISA } } slots. } } } } Warren S. } } } } } } -----Original Message----- } } X-from: kenconverse-at-qualityimages.biz } } [mailto:kenconverse-at-qualityimages.biz] } } Sent: Wednesday, March 04, 2009 9:10 AM } } To: wesaia-at-iastate.edu } } Subject: [Microscopy] RE: Esem users } } } } ------------------------------------------------------------------- } } ----- } } } } Hi Nikki, } } And there you have it: the number one reason not to abandon a } } pre-PC-controlled SEM - the PC. } } } } I'm still servicing 30+ year old SEMs (at least one approaching 40 } } years) } } that still do yeoman's service day after day and year after year. If } } your } } SEM has the capabilities you need, hang on to it. } } } } I'm sorry that I don't even have any suggestions on what to do } } when the } } vendor runs out of DX4 spares. Perhaps get a boat so that you can } } moorit } } to a very expensive anchor. } } } } If the manufacturers would open their source code, someone who knows } } programming might be able to update the software to a newer PC as } long } } as } } the connection is a network connection rather than proprietary driver } } boards } } for an obsolete bus. } } } } It is up to the users to demand a longer view towards these 6 (and 7) } } figure } } investments. } } } } Ken Converse } } owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } DISCLAIMER: Quality Images provides both per call and full service } } contracts on non-PC-controlled SEMs in the northeastern United } States. } } } } } } -----Original Message----- } } X-from: Nicola.Weston-at-nottingham.ac.uk } } [mailto:Nicola.Weston-at-nottingham.ac.uk] } } } } Hi } } I think I should clarify that we have a service contract & good } } supportfrom FEI. My main concern is the integrated DX4 pc, which } } has been } } upgraded as far as possible. So far we have been able to buy parts } } fromFEI but I am worried about future sourcing of spares for our } } system as } } parts are no longer made. } } It's good to know about second hand supplies from elsewhere though, } } thanks for all your replies. } } } } Regards } } Nikki } } } } } } } } Nikki } } } } Are you saying that FEI will not sell you the parts? } } } } regards, } } } } Jim } } } } } } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: } } Mon, 2 } } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } } } From:Nicola.Weston-at-nottingham.ac.uk } Reply-to: } } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy } } Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] } } ESEM users } } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } X-lewp: MicroscopyListSpam NAGS } } } } } ------------------------------------------------------------------- } } ----- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------- } } ----- } } } } } } Hello } } } We have a FEI XL30 ESEM-FEG dating back a few years now and are } } } } } beginning to encounter problems with sourcing spare parts. Just } } } wondering if anyone else out there is running an xl30 esem and if } } so are } } } you having similar problems now they are no longer made,or does } } everyone } have a Quanta these days? } } } } } } Thanks } } } Nikki Weston } } } School M3 } } } University of Nottingham } } } } This message has been checked for viruses but the contents of an } } attachment } } may still contain software viruses, which could damage your computer } } system: } } you are advised to perform your own checks. Email communications with } } the } } University of Nottingham may be monitored as permitted by UK } } legislation. } } } } } } } } ==============================Original } } Headers==============================31, 36 -- From } } wesaia-at-iastate.edu Wed Mar 4 09:28:15 2009 } } 31, 36 -- Received: from mailhub-4.iastate.edu (mailhub- } } 4.iastate.edu [129.186.140.14]) } } 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id n24FSFIW022376 } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 } } 09:28:15 -0600 } } 31, 36 -- Received: from devirus-10.iastate.edu (devirus- } } 10.iastate.edu [129.186.1.47]) } } 31, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) } } with SMTP id n24FSETv025573 } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 } } 09:28:14 -0600 } } 31, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) } } by devirus-10.iastate.edu with smtp } } 31, 36 -- id 60e4_98b2ddf2_08ce_11de_b941_00137253420a; 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==============================Original Headers============================== 9, 18 -- From dljones-at-bestweb.net Wed Mar 4 14:05:40 2009 9, 18 -- Received: from smtp3.bestweb.net (smtp3.bestweb.net [209.94.103.43]) 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24K5ebE005833 9, 18 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 14:05:40 -0600 9, 18 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202]) 9, 18 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 18 -- (No client certificate requested) 9, 18 -- by smtp3.bestweb.net (Postfix) with ESMTPS id C97145C67 9, 18 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:05:39 -0500 (EST) 9, 18 -- Date: Wed, 4 Mar 2009 19:37:30 +0000 (UTC) 9, 18 -- From: dljones {dljones-at-bestweb.net} 9, 18 -- To: microscopy-at-microscopy.com 9, 18 -- Subject: Re: [Microscopy] Re: Esem users 9, 18 -- In-Reply-To: {200903041928.n24JSY66008245-at-ns.microscopy.com} 9, 18 -- Message-ID: {Pine.BSF.4.64.0903041923470.32551-at-monet.bestweb.net} 9, 18 -- References: {200903041928.n24JSY66008245-at-ns.microscopy.com} 9, 18 -- MIME-Version: 1.0 9, 18 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
This is an interesting discussion. I'm pleased to see that I'm not the only person with deep- seated reservations about computerised instrumentation. It costs a fortune to get anything professionally serviced down here as real expertise is often only to be found in Australia, and by the time accomodation, travel cost, and travel time is added, a one-day visit can cost thousands of our shrinking dollars.
Australia and New Zealand are not, in fact, all that close together!
My 1987 JEOL 840 has a totally mysterious and enigmatic CPU embedded deep within it, being, I guess the early days of microcomputerisation, the documentation has only an occasional reference to it.
I live in fear of it malfunctioning but am comforted somewhat by the amazing reliability of the JEOL electronics.
If it dies or even gets sick, is that the end for the instrument?
Anybody been there with an 840?
cheers Ritchie Sims
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 14, 30 -- From r.sims-at-auckland.ac.nz Wed Mar 4 14:21:54 2009 14, 30 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34]) 14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24KLrs3026200 14, 30 -- for {microscopy-at-Microscopy.Com} ; Wed, 4 Mar 2009 14:21:53 -0600 14, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3EA991AA7A 14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:52 +1300 (NZDT) 14, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 14, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 14, 30 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 14, 30 -- with ESMTP id VWtYifBHddk0 for {microscopy-at-Microscopy.Com} ; 14, 30 -- Thu, 5 Mar 2009 09:21:52 +1300 (NZDT) 14, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 14, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) 14, 30 -- (No client certificate requested) 14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1F5AA1AA70 14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:51 +1300 (NZDT) 14, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 14, 30 -- To: microscopy-at-Microscopy.Com 14, 30 -- Date: Thu, 05 Mar 2009 09:24:00 +1300 14, 30 -- MIME-Version: 1.0 14, 30 -- Subject: Re: [Microscopy] Esem users 14, 30 -- Message-ID: {49AF9A30.12078.415D66-at-r.sims.auckland.ac.nz} 14, 30 -- Priority: normal 14, 30 -- In-reply-to: {200903042006.n24K6SAt008123-at-ns.microscopy.com} 14, 30 -- References: {200903042006.n24K6SAt008123-at-ns.microscopy.com} 14, 30 -- X-mailer: Pegasus Mail for Windows (4.41) 14, 30 -- Content-type: text/plain; charset=US-ASCII 14, 30 -- Content-transfer-encoding: 7BIT 14, 30 -- Content-description: Mail message body ==============================End of - Headers==============================
} Dear Malcolm, } I recently upgraded my Hitachi S3000N computer and it was not a problem, just cost money and required a service call, because there are things to change inside the SEM, as well as the computer and monitor. The computer has to be configured in Japan. The new system boots very fast, I got new software that is a bit nicer and I won't be obselete for another few years. } On this thread, one of the reasons that the SEMs are all run on consumer computers, besides the fact that customers demanded it, is that it is way cheaper. Turn knobs, pots and other discrete components are expensive, now, compared to software, which gets cheaper the more systems you install. } My two cents worth. } Regards, } Mary Fletcher } } } } -----Original Message----- } } } Date: Wed Mar 04 11:31:51 PST 2009 } } From: malcolm.haswell-at-sunderland.ac.uk } } Subject: [Microscopy] Re: Esem users } } To: maryflet-at-interchange.ubc.ca } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Warren } } } } this does raise one or two interesting points about computers in } } microscopy. } } } } We fairly easily upgraded the operating system on our Link EDS system } } several years ago but had a lot of problems even discussing the use of } } new printer drivers for our Hitachi S3000N. } } } } I get the impression that microscope control systems and imaging are } } so heavily integrated into their computers that a simple upgrade is } } not possible because of the complexity of hardware, bespoke drivers, } } special software and the operating system and peripherals. Maybe this } } will change with 2nd and 3rd generation systems. } } } } Analytical EDS systems which have been computerized for years seem a } } bit easier because they have less integrated controls, no active } } imaging and software packages seem a bit more portable. } } } } Maybe as well as looking out for sales of microscopy spares it might } } be useful to keep a couple of legacy computers for the technologies } } that won't migrate onto newer computers. } } } } Malcolm } } } } Malcolm Haswell } } Electron Microscope Unit } } Faculty of Applied Sciences } } University of Sunderland } } SUNDERLAND } } SR1 3SD } } UK } } } } email: malcolm.haswell-at-sunderland.ac.uk } } } } } } ----- Original Message ----- } } X-from: wesaia-at-iastate.edu } } Date: Wednesday, March 4, 2009 3:33 pm } } Subject: [Microscopy] Esem users } } } } } } } } } } } } } } ------------------------------------------------------------------- } } } --------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } AmericaTo Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserverOn-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------ } } } ---------------------------------------------------------------- } } } } } } Nikki, how embedded is that PC? How peculiar is it? } } } } } } I would think if you have a decent computer person around, you could } } } upgrade on your own. There could be timing issues and you might need } } } support for an ISA slot or two, but you should be able to do a } } } lot. I } } } would not count on the manufacturer for help. } } } } } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 } } } to a } } } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us } } along } } } the way and would not guarantee that our upgrade would work. They had } } } not tested our exact hardware and could not guarantee success. But } } } neither did they promise failure. The upgrade worked fine. We do know } } } that the software breaks when we upgrade to Windows XP, so we are } } } maxedout with the OS if not the hardware. Fortunately, the } } } hardware is } } } current and stable and snappy as it is. } } } } } } If you do need a replacement DX4, they are still out there on the } } } market. I found several links to ones. I might even have one } } gathering } } } dust at home. I know I have several older, working PCs with ISA } } } slots. } } } } } } Warren S. } } } } } } } } } -----Original Message----- } } } X-from: kenconverse-at-qualityimages.biz } } } [mailto:kenconverse-at-qualityimages.biz] } } } Sent: Wednesday, March 04, 2009 9:10 AM } } } To: wesaia-at-iastate.edu } } } Subject: [Microscopy] RE: Esem users } } } } } } ------------------------------------------------------------------- } } } ----- } } } } } } Hi Nikki, } } } And there you have it: the number one reason not to abandon a } } } pre-PC-controlled SEM - the PC. } } } } } } I'm still servicing 30+ year old SEMs (at least one approaching 40 } } } years) } } } that still do yeoman's service day after day and year after year. If } } } your } } } SEM has the capabilities you need, hang on to it. } } } } } } I'm sorry that I don't even have any suggestions on what to do } } } when the } } } vendor runs out of DX4 spares. Perhaps get a boat so that you can } } } moorit } } } to a very expensive anchor. } } } } } } If the manufacturers would open their source code, someone who knows } } } programming might be able to update the software to a newer PC as } } long } } } as } } } the connection is a network connection rather than proprietary driver } } } boards } } } for an obsolete bus. } } } } } } It is up to the users to demand a longer view towards these 6 (and 7) } } } figure } } } investments. } } } } } } Ken Converse } } } owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } Fax 207-647-2688 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } DISCLAIMER: Quality Images provides both per call and full service } } } contracts on non-PC-controlled SEMs in the northeastern United } } States. } } } } } } } } } -----Original Message----- } } } X-from: Nicola.Weston-at-nottingham.ac.uk } } } [mailto:Nicola.Weston-at-nottingham.ac.uk] } } } } } } Hi } } } I think I should clarify that we have a service contract & good } } } supportfrom FEI. My main concern is the integrated DX4 pc, which } } } has been } } } upgraded as far as possible. So far we have been able to buy parts } } } fromFEI but I am worried about future sourcing of spares for our } } } system as } } } parts are no longer made. } } } It's good to know about second hand supplies from elsewhere though, } } } thanks for all your replies. } } } } } } Regards } } } Nikki } } } } } } } } } } } } Nikki } } } } } } Are you saying that FEI will not sell you the parts? } } } } } } regards, } } } } } } Jim } } } } } } } } } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: } } } Mon, 2 } } } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } } } } From:Nicola.Weston-at-nottingham.ac.uk } Reply-to: } } } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy } } } Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] } } } ESEM users } } } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } ------------------------------------------------------------------- } } } ----- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ------------------------------------------------------------------- } } } ----- } } } } } } } } Hello } } } } We have a FEI XL30 ESEM-FEG dating back a few years now and are } } } } } } } beginning to encounter problems with sourcing spare parts. Just } } } } wondering if anyone else out there is running an xl30 esem and if } } } so are } } } } you having similar problems now they are no longer made,or does } } } everyone } have a Quanta these days? } } } } } } } } Thanks } } } } Nikki Weston } } } } School M3 } } } } University of Nottingham } } } } } } This message has been checked for viruses but the contents of an } } } attachment } } } may still contain software viruses, which could damage your computer } } } system: } } } you are advised to perform your own checks. Email communications with } } } the } } } University of Nottingham may be monitored as permitted by UK } } } legislation. } } } } } } } } } } } } ==============================Original } } } Headers==============================31, 36 -- From } } } wesaia-at-iastate.edu Wed Mar 4 09:28:15 2009 } } } 31, 36 -- Received: from mailhub-4.iastate.edu (mailhub- } } } 4.iastate.edu [129.186.140.14]) } } } 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id n24FSFIW022376 } } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 } } } 09:28:15 -0600 } } } 31, 36 -- Received: from devirus-10.iastate.edu (devirus- } } } 10.iastate.edu [129.186.1.47]) } } } 31, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) } } } with SMTP id n24FSETv025573 } } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 } } } 09:28:14 -0600 } } } 31, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) } } } by devirus-10.iastate.edu with smtp } } } 31, 36 -- id 60e4_98b2ddf2_08ce_11de_b941_00137253420a; 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==============================Original Headers============================== 2, 26 -- From maryflet-at-interchange.ubc.ca Wed Mar 4 14:40:14 2009 2, 26 -- Received: from mr4.mail-relay.ubc.ca (mr4.mail-relay.ubc.ca [137.82.45.7]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24KeDSM007886 2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 14:40:14 -0600 2, 26 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 2, 26 -- by mr4.mail-relay.ubc.ca (Postfix) with ESMTP id 9033C1B262 2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:40:12 -0800 (PST) 2, 26 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14]) 2, 26 -- by smtp.interchange.ubc.ca 2, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 2, 26 -- with ESMTP id {0KG0003BW1BVKT-at-smtp.interchange.ubc.ca} for 2, 26 -- microscopy-at-microscopy.com; Wed, 04 Mar 2009 12:40:12 -0800 (PST) 2, 26 -- Date: Wed, 04 Mar 2009 12:40:11 -0800 (PST) 2, 26 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 2, 26 -- Subject: Fwd: Re: [Microscopy] Re: Esem users 2, 26 -- To: microscopy-at-microscopy.com 2, 26 -- Message-id: {15129506.7991236199211456.JavaMail.myubc2-at-handel.my.ubc.ca} 2, 26 -- MIME-version: 1.0 2, 26 -- X-Mailer: uPortal WEB email client 3.0 2, 26 -- Content-type: text/plain; charset=us-ascii 2, 26 -- Content-transfer-encoding: 7bit 2, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.3.4.202241 2, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 2, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_10000_PLUS 0, OEM_SOFTWARE_X1 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0, __PHISH_SPEAR_REASONS 0, __PHISH_SPEAR_STRUCTURE_1 0, __SANE_MSGID 0 2, 26 -- X-Spam-Level: 2, 26 -- X-Spam-Flag: No ==============================End of - Headers==============================
As it often happens there is truth to both sides of the story. Inherently digital operations, like image processing, are done and best controlled by computers; routine and well established procedures are more efficient with automated macros and pre-saved settings, also computer is less likely to forget about safety checks for stage travel limits, etc... (of cause after all bugs were worked out).
But it is probably easier to develop "feel" for inherently "manual" operations, like alignments or stigmation for example, by turning knobs (even though automated focus/stig works most of the times on most of the tools). It is also likely that "non-standard" research applications may call for settings and combinations of adjustments unforeseen by GUI programmer (or the system engineer who wrote SOW for him/her).
Digital image processing is a big part of the tool capabilities and requires computer, but keeping in mind that equipment manufacturers are first and foremost money-making organizations becomes easier to see another (maybe main?) factor behind heavier reliance on GUI software and elimination of knob controls in some instruments - manufacturing costs of GUI are zero! Computer is part of the system already, and after the development is done and its expenses written off - there is no extra hardware to build into the machine, making BOM and manufacturing books look better. Modifications of GUI are also far less expensive then modifying hard-wired knob panel.
Because of hardware costs factor, future instruments will be even more computer-based, but IMHO (which did not change in last 10 years) instruments intended for serious work should have both GUI and the knob controls available. Almost impossible to foresee all the applications where research tool will be used, and limiting controls to only the GUI has danger of locking operator in the inherently limited box of pre-defined capabilities, which may actually be good in some situations but very frustrating in others. There are ways to keep both "knob turning" and "mouse clicking" users happy and instrument controls both convenient and flexible, just takes some thinking and occasional look "out of the box".
Just my 2C, not that anybody cares :)
Cheers, Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] Sent: Wednesday, March 04, 2009 2:06 PM To: vray-at-partbeamsystech.com
Hi all, Just an alternative perspective.
Maybe I'm just a computer geek or maybe it's the FEI interface (which I find it simple, easy and intuitive), but even with the dedicated knob set (which I insisted on when we bought the instrument, having been an "analog knob feel" junkie myself), I find I only ever use the big mag knob to zoom in or out.
The mouse based focus and stig I find effortless to use and very responsive (it's right button click and drag (in X) to focus and shift right click and drag (in X and Y) to stigmate).
I am completely won over by the FEI Quanta ESEM computer control much to my surprise. john
At 10:40 AM 3/4/2009, tina-at-pbrc.hawaii.edu wrote: } --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 21 -- From donovan-at-uoregon.edu Wed Mar 4 13:04:01 2009 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24J40vi031984 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 13:04:00 -0600 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 8, 21 -- (authenticated bits=0) 8, 21 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n24J3x0h006753 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 11:04:00 -0800 8, 21 -- Message-Id: {200903041904.n24J3x0h006753-at-smtp.uoregon.edu} 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Wed, 04 Mar 2009 11:03:49 -0800 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} 8, 21 -- Subject: Re: [Microscopy] Esem users 8, 21 -- In-Reply-To: {200903041840.n24IewXj025217-at-ns.microscopy.com} 8, 21 -- References: {200903041840.n24IewXj025217-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 21 -- X-Virus-Scanned: ClamAV 0.94.2/9067/Wed Mar 4 02:06:09 2009 on mserv1 8, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 24, 25 -- From vray-at-partbeamsystech.com Wed Mar 4 15:27:24 2009 24, 25 -- Received: from smtp107.biz.mail.re2.yahoo.com (smtp107.biz.mail.re2.yahoo.com [206.190.52.176]) 24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n24LRNj9022890 24, 25 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:27:23 -0600 24, 25 -- Message-Id: {200903042127.n24LRNj9022890-at-ns.microscopy.com} 24, 25 -- Received: (qmail 85032 invoked from network); 4 Mar 2009 21:27:23 -0000 24, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.106.16 with login) 24, 25 -- by smtp107.biz.mail.re2.yahoo.com with SMTP; 4 Mar 2009 21:27:22 -0000 24, 25 -- X-YMail-OSG: PFBAEh8VM1k1q90G9L5.8EGg0rigdp1kQIk4DQeV0h5I4TZowkoiFr7L2QIB3ZfWznaI6mBtpodXD54RP4pLzeZTslWP7vxxGIlz4Sn0TsSRm9ylqYY7Rjv8DJPqtRavepH8cajRyIfUnv.psQLty1FPilrzi9DzsV1Qvxl7LXP2utl0zMcdByqK7UyW3vdSnXqBZxvIigjZt6kOeA_kVylnd1.mcMtb 24, 25 -- X-Yahoo-Newman-Property: ymail-3 24, 25 -- Reply-To: {vray-at-partbeamsystech.com} 24, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 24, 25 -- To: {donovan-at-uoregon.edu} 24, 25 -- Cc: {microscopy-at-microscopy.com} 24, 25 -- Subject: RE: [Microscopy] Re: Esem users 24, 25 -- Date: Wed, 4 Mar 2009 16:30:16 -0500 24, 25 -- Organization: PBST / MEO Engineering 24, 25 -- MIME-Version: 1.0 24, 25 -- Content-Type: text/plain; 24, 25 -- charset="us-ascii" 24, 25 -- Content-Transfer-Encoding: 7bit 24, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 24, 25 -- Thread-Index: Acmc/DHrG15s9TNDTR2svDzY8R2yhAAB7NvQ 24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 24, 25 -- In-Reply-To: {200903041905.n24J5VPs002606-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi Mary, You're right that the electromechanical (and optomechanical) components are more expensive, but they can at least be replaced (and usually easily trouble-shot) and sometimes repaired on site. Most last for many years without any problems (and they don't require upgrades).
When it costs $25-30,000 to replace a $2000 computer with a $1000 computer that is already out of date when it's installed would give me serious pause. That is the price range of upgrades that I've heard about. I don't know what yours cost.
I have resigned myself (sadly) to the fact that the computers I use are mere commodities that are often cheaper to replace than to try and repair. I have some difficulty coming to the same conclusion on equipment that costs as much as an SEM, uses the resources that are used in making an SEM, and whose main components are built to last decades. It just doesn't make sense economically, environmentally or socially to just chuck this stuff out when the basic PC morphs so fast and (previous comments not withstanding) can no longer run the equipment due to previously discussed design parameters (that should be changed, as discussed in a thread a few months ago).
Maybe Hitachi is paying attention and that is why the upgrade went so smoothly, although you haven't said what that $1000 computer cost.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: maryflet-at-interchange.ubc.ca [mailto:maryflet-at-interchange.ubc.ca] Sent: Wednesday, March 04, 2009 3:42 PM To: kenconverse-at-qualityimages.biz
} Dear Malcolm, } I recently upgraded my Hitachi S3000N computer and it was not a problem, just cost money and required a service call, because there are things to change inside the SEM, as well as the computer and monitor. The computer has to be configured in Japan. The new system boots very fast, I got new software that is a bit nicer and I won't be obselete for another few years. } On this thread, one of the reasons that the SEMs are all run on consumer computers, besides the fact that customers demanded it, is that it is way cheaper. Turn knobs, pots and other discrete components are expensive, now, compared to software, which gets cheaper the more systems you install. } My two cents worth. } Regards, } Mary Fletcher } } } } -----Original Message----- } } } Date: Wed Mar 04 11:31:51 PST 2009 } } From: malcolm.haswell-at-sunderland.ac.uk } } Subject: [Microscopy] Re: Esem users } } To: maryflet-at-interchange.ubc.ca } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Warren } } } } this does raise one or two interesting points about computers in } } microscopy. } } } } We fairly easily upgraded the operating system on our Link EDS system } } several years ago but had a lot of problems even discussing the use of } } new printer drivers for our Hitachi S3000N. } } } } I get the impression that microscope control systems and imaging are } } so heavily integrated into their computers that a simple upgrade is } } not possible because of the complexity of hardware, bespoke drivers, } } special software and the operating system and peripherals. Maybe this } } will change with 2nd and 3rd generation systems. } } } } Analytical EDS systems which have been computerized for years seem a } } bit easier because they have less integrated controls, no active } } imaging and software packages seem a bit more portable. } } } } Maybe as well as looking out for sales of microscopy spares it might } } be useful to keep a couple of legacy computers for the technologies } } that won't migrate onto newer computers. } } } } Malcolm } } } } Malcolm Haswell } } Electron Microscope Unit } } Faculty of Applied Sciences } } University of Sunderland } } SUNDERLAND } } SR1 3SD } } UK } } } } email: malcolm.haswell-at-sunderland.ac.uk } } } } } } ----- Original Message ----- } } X-from: wesaia-at-iastate.edu } } Date: Wednesday, March 4, 2009 3:33 pm } } Subject: [Microscopy] Esem users } } } } } } } } } } } } } } ------------------------------------------------------------------- } } } --------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } AmericaTo Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserverOn-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------ } } } ---------------------------------------------------------------- } } } } } } Nikki, how embedded is that PC? How peculiar is it? } } } } } } I would think if you have a decent computer person around, you could } } } upgrade on your own. There could be timing issues and you might need } } } support for an ISA slot or two, but you should be able to do a } } } lot. I } } } would not count on the manufacturer for help. } } } } } } We upgraded our EDS system from a 50 MHz 486-DX2 running Windows 3 } } } to a } } } 1800 MHz AMD running Windows 2000. The manufacturer cautioned us } } along } } } the way and would not guarantee that our upgrade would work. They had } } } not tested our exact hardware and could not guarantee success. But } } } neither did they promise failure. The upgrade worked fine. We do know } } } that the software breaks when we upgrade to Windows XP, so we are } } } maxedout with the OS if not the hardware. Fortunately, the } } } hardware is } } } current and stable and snappy as it is. } } } } } } If you do need a replacement DX4, they are still out there on the } } } market. I found several links to ones. I might even have one } } gathering } } } dust at home. I know I have several older, working PCs with ISA } } } slots. } } } } } } Warren S. } } } } } } } } } -----Original Message----- } } } X-from: kenconverse-at-qualityimages.biz } } } [mailto:kenconverse-at-qualityimages.biz] } } } Sent: Wednesday, March 04, 2009 9:10 AM } } } To: wesaia-at-iastate.edu } } } Subject: [Microscopy] RE: Esem users } } } } } } ------------------------------------------------------------------- } } } ----- } } } } } } Hi Nikki, } } } And there you have it: the number one reason not to abandon a } } } pre-PC-controlled SEM - the PC. } } } } } } I'm still servicing 30+ year old SEMs (at least one approaching 40 } } } years) } } } that still do yeoman's service day after day and year after year. If } } } your } } } SEM has the capabilities you need, hang on to it. } } } } } } I'm sorry that I don't even have any suggestions on what to do } } } when the } } } vendor runs out of DX4 spares. Perhaps get a boat so that you can } } } moorit } } } to a very expensive anchor. } } } } } } If the manufacturers would open their source code, someone who knows } } } programming might be able to update the software to a newer PC as } } long } } } as } } } the connection is a network connection rather than proprietary driver } } } boards } } } for an obsolete bus. } } } } } } It is up to the users to demand a longer view towards these 6 (and 7) } } } figure } } } investments. } } } } } } Ken Converse } } } owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } Fax 207-647-2688 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } DISCLAIMER: Quality Images provides both per call and full service } } } contracts on non-PC-controlled SEMs in the northeastern United } } States. } } } } } } } } } -----Original Message----- } } } X-from: Nicola.Weston-at-nottingham.ac.uk } } } [mailto:Nicola.Weston-at-nottingham.ac.uk] } } } } } } Hi } } } I think I should clarify that we have a service contract & good } } } supportfrom FEI. My main concern is the integrated DX4 pc, which } } } has been } } } upgraded as far as possible. So far we have been able to buy parts } } } fromFEI but I am worried about future sourcing of spares for our } } } system as } } } parts are no longer made. } } } It's good to know about second hand supplies from elsewhere though, } } } thanks for all your replies. } } } } } } Regards } } } Nikki } } } } } } } } } } } } Nikki } } } } } } Are you saying that FEI will not sell you the parts? } } } } } } regards, } } } } } } Jim } } } } } } } } } } From mail-at-ns.microscopy.com Mon Mar 2 05:06:59 2009 } Date: } } } Mon, 2 } } } Mar 2009 04:07:29 -0600 } To: jquinn-at-www.matscieng.sunysb.edu } } } } From:Nicola.Weston-at-nottingham.ac.uk } Reply-to: } } } Nicola.Weston-at-nottingham.ac.uk } X-Resent-From: "Microscopy } } } Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] } } } ESEM users } } } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } } X-lewp: MicroscopyListSpam NAGS } } } } } } } ------------------------------------------------------------------- } } } ----- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ------------------------------------------------------------------- } } } ----- } } } } } } } } Hello } } } } We have a FEI XL30 ESEM-FEG dating back a few years now and are } } } } } } } beginning to encounter problems with sourcing spare parts. Just } } } } wondering if anyone else out there is running an xl30 esem and if } } } so are } } } } you having similar problems now they are no longer made,or does } } } everyone } have a Quanta these days? } } } } } } } } Thanks } } } } Nikki Weston } } } } School M3 } } } } University of Nottingham } } } } } } This message has been checked for viruses but the contents of an } } } attachment } } } may still contain software viruses, which could damage your computer } } } system: } } } you are advised to perform your own checks. Email communications with } } } the } } } University of Nottingham may be monitored as permitted by UK } } } legislation. } } } } } } } } } } } } ==============================Original } } } Headers==============================31, 36 -- From } } } wesaia-at-iastate.edu Wed Mar 4 09:28:15 2009 } } } 31, 36 -- Received: from mailhub-4.iastate.edu (mailhub- } } } 4.iastate.edu [129.186.140.14]) } } } 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id n24FSFIW022376 } } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 } } } 09:28:15 -0600 } } } 31, 36 -- Received: from devirus-10.iastate.edu (devirus- } } } 10.iastate.edu [129.186.1.47]) } } } 31, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) } } } with SMTP id n24FSETv025573 } } } 31, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 } } } 09:28:14 -0600 } } } 31, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) } } } by devirus-10.iastate.edu with smtp } } } 31, 36 -- id 60e4_98b2ddf2_08ce_11de_b941_00137253420a; 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==============================Original Headers============================== 2, 26 -- From maryflet-at-interchange.ubc.ca Wed Mar 4 14:40:14 2009 2, 26 -- Received: from mr4.mail-relay.ubc.ca (mr4.mail-relay.ubc.ca [137.82.45.7]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24KeDSM007886 2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 14:40:14 -0600 2, 26 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 2, 26 -- by mr4.mail-relay.ubc.ca (Postfix) with ESMTP id 9033C1B262 2, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 12:40:12 -0800 (PST) 2, 26 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14]) 2, 26 -- by smtp.interchange.ubc.ca 2, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 2, 26 -- with ESMTP id {0KG0003BW1BVKT-at-smtp.interchange.ubc.ca} for 2, 26 -- microscopy-at-microscopy.com; Wed, 04 Mar 2009 12:40:12 -0800 (PST) 2, 26 -- Date: Wed, 04 Mar 2009 12:40:11 -0800 (PST) 2, 26 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 2, 26 -- Subject: Fwd: Re: [Microscopy] Re: Esem users 2, 26 -- To: microscopy-at-microscopy.com 2, 26 -- Message-id: {15129506.7991236199211456.JavaMail.myubc2-at-handel.my.ubc.ca} 2, 26 -- MIME-version: 1.0 2, 26 -- X-Mailer: uPortal WEB email client 3.0 2, 26 -- Content-type: text/plain; charset=us-ascii 2, 26 -- Content-transfer-encoding: 7bit 2, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.3.4.202241 2, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 2, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_10000_PLUS 0, OEM_SOFTWARE_X1 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0, __PHISH_SPEAR_REASONS 0, __PHISH_SPEAR_STRUCTURE_1 0, __SANE_MSGID 0 2, 26 -- X-Spam-Level: 2, 26 -- X-Spam-Flag: No ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From kenconverse-at-qualityimages.biz Wed Mar 4 15:35:00 2009 17, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n24LZ0Fc004165 17, 26 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 15:35:00 -0600 17, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com 17, 26 -- with ESMTP 17, 26 -- id {20090304213459.VUDA5856.cdptpa-omta04.mail.rr.com-at-Ken} ; 17, 26 -- Wed, 4 Mar 2009 21:34:59 +0000 17, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 17, 26 -- To: {maryflet-at-interchange.ubc.ca} , 17, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 17, 26 -- Subject: RE: [Microscopy] Fwd: Esem users 17, 26 -- Date: Wed, 4 Mar 2009 16:34:53 -0500 17, 26 -- Message-ID: {A57062B199364760B43D652B5D534AAF-at-Ken} 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="us-ascii" 17, 26 -- X-Priority: 3 (Normal) 17, 26 -- X-MSMail-Priority: Normal 17, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 17, 26 -- Importance: Normal 17, 26 -- Thread-Index: AcmdCbq9IMl2+RlVTbipBGq7mXgWCQABGAdQ 17, 26 -- In-Reply-To: {200903042042.n24KgPHu011869-at-ns.microscopy.com} 17, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n24LZ0Fc004165 ==============================End of - Headers==============================
If you're in a dry climate like Canberra (unlike the wet murk of AKL), sadly, your 2-3-decade old JEOL boards have started to crack and it takes quite a while to find the dry join that needs re-soldering on those big boards - and the admin rulers of the roost will not fork out for maintenance any more. And the most well-used knobs and dials have worn out or are wearing out and some are hard to replace. A great old instrument brought down by incremental decline.
But in our new SEM (symbiotically linked to computer) two of the main motherboards spat the dummy within a month(!) and of course, the instrument was unusable.... Otherwise, a nice instrument.....
cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 5/03/09 7:27 AM, "r.sims-at-auckland.ac.nz" {r.sims-at-auckland.ac.nz} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi } } This is an interesting discussion. I'm pleased to see that I'm not the only } person with deep- } seated reservations about computerised instrumentation. It costs a fortune to } get anything } professionally serviced down here as real expertise is often only to be found } in Australia, and } by the time accomodation, travel cost, and travel time is added, a one-day } visit can cost } thousands of our shrinking dollars. } } Australia and New Zealand are not, in fact, all that close together! } } My 1987 JEOL 840 has a totally mysterious and enigmatic CPU embedded deep } within it, } being, I guess the early days of microcomputerisation, the documentation has } only an } occasional reference to it. } } I live in fear of it malfunctioning but am comforted somewhat by the amazing } reliability of the } JEOL electronics. } } If it dies or even gets sick, is that the end for the instrument? } } Anybody been there with an 840? } } cheers } Ritchie Sims } } } } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 14, 30 -- From r.sims-at-auckland.ac.nz Wed Mar 4 14:21:54 2009 } 14, 30 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz } [130.216.12.34]) } 14, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n24KLrs3026200 } 14, 30 -- for {microscopy-at-Microscopy.Com} ; Wed, 4 Mar 2009 14:21:53 -0600 } 14, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3EA991AA7A } 14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:52 +1300 } (NZDT) } 14, 30 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } 14, 30 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) } 14, 30 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, } port 10024) } 14, 30 -- with ESMTP id VWtYifBHddk0 for {microscopy-at-Microscopy.Com} ; } 14, 30 -- Thu, 5 Mar 2009 09:21:52 +1300 (NZDT) } 14, 30 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz } [130.216.59.18]) } 14, 30 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) } 14, 30 -- (No client certificate requested) } 14, 30 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1F5AA1AA70 } 14, 30 -- for {microscopy-at-Microscopy.Com} ; Thu, 5 Mar 2009 09:21:51 +1300 } (NZDT) } 14, 30 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 14, 30 -- To: microscopy-at-Microscopy.Com } 14, 30 -- Date: Thu, 05 Mar 2009 09:24:00 +1300 } 14, 30 -- MIME-Version: 1.0 } 14, 30 -- Subject: Re: [Microscopy] Esem users } 14, 30 -- Message-ID: {49AF9A30.12078.415D66-at-r.sims.auckland.ac.nz} } 14, 30 -- Priority: normal } 14, 30 -- In-reply-to: {200903042006.n24K6SAt008123-at-ns.microscopy.com} } 14, 30 -- References: {200903042006.n24K6SAt008123-at-ns.microscopy.com} } 14, 30 -- X-mailer: Pegasus Mail for Windows (4.41) } 14, 30 -- Content-type: text/plain; charset=US-ASCII } 14, 30 -- Content-transfer-encoding: 7BIT } 14, 30 -- Content-description: Mail message body } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lbustillos-at-emsl.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Objective for Dispersion Staining
Question: Hello,
I would like to know if anybody knows who can make a centerable central stop in a 10X objective in order to do dispersion staining? I have found objectives that have a central stop but they are permanently mounted. This is not a problem if the central stop is perfectly centered but most of the ones that I have seen are not perfectly centered and this causes problems with trying to see dispersion colors.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both a.l.bleloch-at-liv.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: a.l.bleloch-at-liv.ac.uk Name: Andrew Bleloch
Organization: SuperSTEM Laboratory, UK
Title-Subject: [Filtered] Jobs opportunities at SuperSTEM
Question: There are three posts at various levels available at SuperSTEM in the UK to be found through the following link: http://www.superstem.dl.ac.uk/
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both anita.garg-at-grc.nasa.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM: Electron diffraction Simulation program for PC
Question: Dear Colleagues What is the best electron-diffraction simulation program available for PC these days? Earlier, the "Diffract" program used to have a Mac version only; is there a PC version available now? TIA, Anita
Try McCrone Associates in Westmont, IL. They used to make a centerable center stop just for this purpose, as well as positive and negative dispersion staining. I once had the wonderful experience of acting as a teaching assistant when Dr. McCrone gave a course on this topic to the New York Microscopical Society. These centerable center stops were a bit tricky to work with, but once you get them aligned, they work beautifully.
I also recommend that you contact Dennis O'Leary (rdenol-at-aol.com). He deals in excellent second hand equipment and also has had experience with this technique.
Hope this was helpful.
Best regards, Barbara Foster, President and Sr. Consultant
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now running the FIRST Global study on AFM/STM use. Visit our website for details.
At 07:40 PM 3/4/2009, lbustillos-at-emsl.com wrote:
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==============================Original Headers============================== 14, 24 -- From bfostermme-at-sbcglobal.net Wed Mar 4 20:02:18 2009 14, 24 -- Received: from smtp113.sbc.mail.mud.yahoo.com (smtp113.sbc.mail.mud.yahoo.com [68.142.198.212]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2522ICr013865 14, 24 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 20:02:18 -0600 14, 24 -- Message-Id: {200903050202.n2522ICr013865-at-ns.microscopy.com} 14, 24 -- Received: (qmail 99090 invoked from network); 5 Mar 2009 02:02:17 -0000 14, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 24 -- s=s1024; d=sbcglobal.net; 14, 24 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Cc:In-Reply-To:References:Mime-Version:Content-Type; 14, 24 -- b=aNV5S/8c3QODfD2jdxZTKAUO5t/0du08FvdqNqsRW4WPq5vaXHI45W/Jsi4nBzTajAk9MY7JYHbWuEjPU38q0tGDOenDxTAUNPH2ld16FUd63u03ZWgjGj92aonC8r1ALwCu/Re2A1u6jMQCxNv/xQBj4WrJNWMxx5y3dm9R+P0= ; 14, 24 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login) 14, 24 -- by smtp113.sbc.mail.mud.yahoo.com with SMTP; 5 Mar 2009 02:02:17 -0000 14, 24 -- X-YMail-OSG: LjsP83IVM1nzYytrjTVX20mMwWf9RO4XhWtLaiUdi5FIz8Hm7KWGPJtM9WTWbGvG7YjhBpEyts6f7cJ8iiGXDe9kugDV5Sl2mGf7OAWp2zF9nM7.IkMtWvAhVbL0TMkUBX35pHrD747Nh1hydIMAGH_VR8nLVGi2smQia0zz6x.okTqNlVl33UoGkVdfT5Zi8E2JcGDPqBUQLompE.djEwjLk.CnKmA- 14, 24 -- X-Yahoo-Newman-Property: ymail-3 14, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 24 -- Date: Wed, 04 Mar 2009 19:57:59 -0600 14, 24 -- To: lbustillos-at-emsl.com, microscopy-at-microscopy.com 14, 24 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 14, 24 -- Subject: Re: [Microscopy] viaWWW: Objective for Dispersion Staining 14, 24 -- Cc: "Dennis O'Leary" {rdenol-at-hotmail.com} 14, 24 -- In-Reply-To: {200903050137.n251bFTE006544-at-ns.microscopy.com} 14, 24 -- References: {200903050137.n251bFTE006544-at-ns.microscopy.com} 14, 24 -- Mime-Version: 1.0 14, 24 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
The microscopes I have used so far had some computer interface and all were using some version of Microsoft Windows operating system. Unfortunately, all the systems have crashed from time to time due to computer problems.
In my opinion, Microsoft Windows OS needs to be rebooted periodically, otherwise it is gonna crash in the middle of most important task. And, you are gonna loose your best TEM images!
I have also used Linux operating system for computational work. Linux is much more stable compared to Windows. I wonder if electron microscope vendors ever considered using something like Linux as operating system.
We have a new TEM from FEI and the imaging software has crashed a few times already (this is making me nervous). The computer has only FEI installed software; TEM server, imaging interface etc. No Internet connection either. Hard drives are pretty much empty, as well.
I have to give a break from observation of the sample and save my images frequently. I guess this will be a problem when someone wants to observe and record images of kinematical process e.g. phase change, radiation damage in TEM.
Kind Regards. Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean and! }  only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty. } } Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back. } } My 2 cents Canadian (~1.56 cents US), } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB  E4L 1G7 } CANADA } } phone: 506-364-2519 } fax:  506-364-2505 } email: jehrman-at-mta.ca } www:  http://www.mta.ca/dmf }
==============================Original Headers============================== 12, 32 -- From celikaktas-at-gmail.com Thu Mar 5 01:33:09 2009 12, 32 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.188]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n257X8E3003823 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 01:33:08 -0600 12, 32 -- Received: by fk-out-0910.google.com with SMTP id z23so1377377fkz.2 12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Mar 2009 23:33:07 -0800 (PST) 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 32 -- d=gmail.com; s=gamma; 12, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject 12, 32 -- :from:to:content-type:content-transfer-encoding; 12, 32 -- bh=AYThodq9LiLfTHZnje86wKZbYhgtEia/WM7U/C7u7eo=; 12, 32 -- b=vpUgvbP6P1a+c3ADdQ3obERggm8/tBxXtmTbE4BtWljGYmJM7xBEsfC7oO4sW00YW3 12, 32 -- cwoDHZdHj+3dKsXE68fwbUGnf68tuj+vH+7xHdquQ6ajvNkuxRW9bz5tveZlLnrDNihk 12, 32 -- wSpgxm0W3AkpsAlUqdIbVa/6Pto+ujtFpTJm0= 12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 12, 32 -- d=gmail.com; s=gamma; 12, 32 -- h=mime-version:date:message-id:subject:from:to:content-type 12, 32 -- :content-transfer-encoding; 12, 32 -- b=JQOCZQ0bWfi/cjIj2AHy4LiaVIOvCi9t7ioessoijZ/1MwmapyIDtWXubqnbmupaO9 12, 32 -- AbwDBjHcn/gEyD0ssuOy8oamVu2UFCJYtT0r1/RzBjXBeWt2boQrxSHs66rUtU6fTzbe 12, 32 -- aWSrRRbT90N998YD1QvvNH8auLkyNUw5ir0u0= 12, 32 -- MIME-Version: 1.0 12, 32 -- Received: by 10.103.219.17 with SMTP id w17mr396159muq.36.1236238387799; Wed, 12, 32 -- 04 Mar 2009 23:33:07 -0800 (PST) 12, 32 -- Date: Thu, 5 Mar 2009 10:33:07 +0300 12, 32 -- Message-ID: {1075c5c10903042333i63c8816fh641be80aa3a3a4f7-at-mail.gmail.com} 12, 32 -- Subject: Operating system.. Re: Esem users 12, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} 12, 32 -- To: microscopy {Microscopy-at-microscopy.com} 12, 32 -- Content-Type: text/plain; charset=UTF-8 12, 32 -- Content-Transfer-Encoding: 8bit 12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n257X8E3003823 ==============================End of - Headers==============================
Ayten; My opinion is that FEI tries to do too much with one computer. Our 11 year old JEOL 2010F runs one computer (XP) dedicated to Gatan Digital Micrograph (imaging and EELS), one computer (Win 2000) dedicated to FASTEM, which runs the microscope, and one computer (XP) to run the EDX system (Noran). Ethernet communicates between the three to carry system information like magnification and imaging mode. We NEVER have to reboot these computers and we NEVER have system freezes while running the microscope. Plus, the microscope is operated with knobs after just a few clicks to set it up. Am I just lucky or what?
John Mardinly, Numonyx
-----Original Message----- X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] Sent: Wednesday, March 04, 2009 11:40 PM To: MARDINLY, A
The microscopes I have used so far had some computer interface and all were using some version of Microsoft Windows operating system. Unfortunately, all the systems have crashed from time to time due to computer problems.
In my opinion, Microsoft Windows OS needs to be rebooted periodically, otherwise it is gonna crash in the middle of most important task. And, you are gonna loose your best TEM images!
I have also used Linux operating system for computational work. Linux is much more stable compared to Windows. I wonder if electron microscope vendors ever considered using something like Linux as operating system.
We have a new TEM from FEI and the imaging software has crashed a few times already (this is making me nervous). The computer has only FEI installed software; TEM server, imaging interface etc. No Internet connection either. Hard drives are pretty much empty, as well.
I have to give a break from observation of the sample and save my images frequently. I guess this will be a problem when someone wants to observe and record images of kinematical process e.g. phase change, radiation damage in TEM.
Kind Regards. Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean a! nd! }  only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty. } } Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back. } } My 2 cents Canadian (~1.56 cents US), } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB  E4L 1G7 } CANADA } } phone: 506-364-2519 } fax:  506-364-2505 } email: jehrman-at-mta.ca } www:  http://www.mta.ca/dmf }
==============================Original Headers============================== 12, 32 -- From celikaktas-at-gmail.com Thu Mar 5 01:33:09 2009 12, 32 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.188]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n257X8E3003823 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 01:33:08 -0600 12, 32 -- Received: by fk-out-0910.google.com with SMTP id z23so1377377fkz.2 12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Mar 2009 23:33:07 -0800 (PST) 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 32 -- d=gmail.com; s=gamma; 12, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject 12, 32 -- :from:to:content-type:content-transfer-encoding; 12, 32 -- bh=AYThodq9LiLfTHZnje86wKZbYhgtEia/WM7U/C7u7eo=; 12, 32 -- b=vpUgvbP6P1a+c3ADdQ3obERggm8/tBxXtmTbE4BtWljGYmJM7xBEsfC7oO4sW00YW3 12, 32 -- cwoDHZdHj+3dKsXE68fwbUGnf68tuj+vH+7xHdquQ6ajvNkuxRW9bz5tveZlLnrDNihk 12, 32 -- wSpgxm0W3AkpsAlUqdIbVa/6Pto+ujtFpTJm0= 12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 12, 32 -- d=gmail.com; s=gamma; 12, 32 -- h=mime-version:date:message-id:subject:from:to:content-type 12, 32 -- :content-transfer-encoding; 12, 32 -- b=JQOCZQ0bWfi/cjIj2AHy4LiaVIOvCi9t7ioessoijZ/1MwmapyIDtWXubqnbmupaO9 12, 32 -- AbwDBjHcn/gEyD0ssuOy8oamVu2UFCJYtT0r1/RzBjXBeWt2boQrxSHs66rUtU6fTzbe 12, 32 -- aWSrRRbT90N998YD1QvvNH8auLkyNUw5ir0u0= 12, 32 -- MIME-Version: 1.0 12, 32 -- Received: by 10.103.219.17 with SMTP id w17mr396159muq.36.1236238387799; Wed, 12, 32 -- 04 Mar 2009 23:33:07 -0800 (PST) 12, 32 -- Date: Thu, 5 Mar 2009 10:33:07 +0300 12, 32 -- Message-ID: {1075c5c10903042333i63c8816fh641be80aa3a3a4f7-at-mail.gmail.com} 12, 32 -- Subject: Operating system.. Re: Esem users 12, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} 12, 32 -- To: microscopy {Microscopy-at-microscopy.com} 12, 32 -- Content-Type: text/plain; charset=UTF-8 12, 32 -- Content-Transfer-Encoding: 8bit 12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n257X8E3003823 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 5 02:29:53 2009 21, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n258TrW8018802 21, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 5 Mar 2009 02:29:53 -0600 21, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 21, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 8D05D1440A2; 21, 29 -- Thu, 5 Mar 2009 01:34:05 -0500 (EST) 21, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 21, 29 -- Thu, 5 Mar 2009 03:29:52 -0500 21, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 29 -- Content-class: urn:content-classes:message 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Type: text/plain; 21, 29 -- charset="iso-8859-1" 21, 29 -- Subject: RE: [Microscopy] Operating system.. Re: Esem users 21, 29 -- Date: Thu, 5 Mar 2009 03:29:27 -0500 21, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9EE95D6-at-EXDRESBENMX012.numonyx.local} 21, 29 -- In-Reply-To: {200903050740.n257eMap015286-at-ns.microscopy.com} 21, 29 -- X-MS-Has-Attach: 21, 29 -- X-MS-TNEF-Correlator: 21, 29 -- Thread-Topic: [Microscopy] Operating system.. Re: Esem users 21, 29 -- Thread-Index: AcmdZauU4H31zME3SlyzDz5WmfaahgABTAcg 21, 29 -- References: {200903050740.n257eMap015286-at-ns.microscopy.com} 21, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 21, 29 -- To: {celikaktas-at-gmail.com} 21, 29 -- Cc: {Microscopy-at-Microscopy.com} 21, 29 -- X-OriginalArrivalTime: 05 Mar 2009 08:29:52.0130 (UTC) FILETIME=[8E021620:01C99D6C] 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n258TrW8018802 ==============================End of - Headers==============================
Here is another sad story on a PC controlled microscope. Recently I bought a second hand SEM. At that time I was not a member of the listserver and had no idea about the PC-EM problems. When the graphic card was broken, the microscope¹s company was unable to find a replace one for me, although the scope is no more than 10 years old. Fortunately I found one (actually two) through the web and the scope works again.
But PC problems are continuing. The microscope operating system is one out of tens or maybe hundreds of different versions being around, for the same model! My scope came from the USA with a starting CD of different version than the one already installed in the scope. Although the scope was decommissioned by the company, the company was unable to find for me the correct version. People from the company in Europe don¹t even believe that the scope works on windows 98 and not on NT. A real nightmareŠ
I would like to ask the list if anybody knows somebody who can install a new operating system. A friend in this list told me about a German company that computerizes old microscopes by installing new scan coils. However, if the scope is already computerized like mine, is maybe more difficult.
Also, I would like to propose if there is room for some sort of world-wide union between EM owners who have PC controlled scopes of several yeas old. Such a union could help giving solutions outside the microscope companies or even demand from the companies to keep PC spare parts for longer time etc.
Let me kick another thought into this discussion: most companies management wants computerized equipment. Why? They perceive then as less expensive to operate. No need for several experts at high salary and benefits, perhaps not even one. The tech rep sets it up and each user just walks up and uses it. Can't screw up the settings, cause the computer "remembers" the original settings.
I've see this with GC/MS, with IR, with image analysis equipment with TEMs and SEMs. Even the adds for this equipment suggest at "expert in a box" with their equipment.
now I'm not saying they are total to blame... I've heard sales pitches to management about justification for a piece of equipment by claiming the ease of operation in a multi-user environment: "blab blab..free standing, user friendly and we don't need dedicated operators....blab blab." I suspect we've even used this argument to get equipment we needed.
I needed a new EDS system at Degussa, I didn't select the best, I selected one that was the most user friendly 'cause I could justify it.
So sign me up for the Luddite Microscopy Group (humm..maybe a new Linked-In group), but I know to some small degree I'm responsible.
stay safe....... Frank Reformed Luddite Microscopist
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==============================Original Headers============================== 8, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 5 06:05:01 2009 8, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25C515R019105 8, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 06:05:01 -0600 8, 22 -- In-Reply-To: {200903051057.n25AvaOA016153-at-ns.microscopy.com} 8, 22 -- Subject: Re: other not so sad story 8, 22 -- To: Microscopy-at-microscopy.com 8, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 8, 22 -- Message-ID: {OFE15843D7.2A79266A-ON85257570.0040DCFA-85257570.00425949-at-lincolnelectric.com} 8, 22 -- Date: Thu, 5 Mar 2009 07:04:42 -0500 8, 22 -- From: Frank_Karl-at-lincolnelectric.com 8, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM, 8, 22 -- CD-MIME complete at 03/05/2009 07:04:43 AM, 8, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM, 8, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM, 8, 22 -- Serialize complete at 03/05/2009 07:04:43 AM 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: text/plain; 8, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
if you're talking about the upgrade from Windows NT 4 to XP then I am aware of it and if all goes well we hope to arrange it in the future. In fact, despite the cost (probably about 1/10 of original microscope), I get the impression that not all manufacturers offer similar upgrades so full marks to Hitachi.
I hasten to add that I have no association with Hitachi other than as a satisfied customer.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Mary Fletcher {maryflet-at-interchange.ubc.ca}
Hi, Yorgos,
SemTech Solutions, near Boston, "rehabilitates" old EMs but gutting the computer system and updating them. I don't know if they do any work outside the US but they have an excellent reputation. Contact Gerry O'Loughlin (gerry-at-semtechsolutions.com).
Hope this was helpful. Barbara Foster
Use AFM/ STM/ or NSOM? Take part in the first GLOBAL AFM/STM study. Deatails at www.MicroscopyEducation.com
Barbara Foster, President and Sr. Consultant Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
/At 05:00 AM 3/5/2009, you wrote:
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==============================Original Headers============================== 12, 25 -- From bfostermme-at-sbcglobal.net Thu Mar 5 07:19:20 2009 12, 25 -- Received: from smtp103.sbc.mail.mud.yahoo.com (smtp103.sbc.mail.mud.yahoo.com [68.142.198.202]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n25DJHBl015568 12, 25 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 07:19:19 -0600 12, 25 -- Message-Id: {200903051319.n25DJHBl015568-at-ns.microscopy.com} 12, 25 -- Received: (qmail 41944 invoked from network); 5 Mar 2009 13:19:16 -0000 12, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 25 -- s=s1024; d=sbcglobal.net; 12, 25 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Cc:In-Reply-To:References:Mime-Version:Content-Type:Content-Transfer-Encoding; 12, 25 -- b=Il1htlMG/N2goJu87W7deXzWKfKgL6L0vwHO3MblP69vRZZCxBCVizeuX7wKNtMRtg4sz0KBxXelw9v0t3M4mQa8S2rFDGAj4FIBtZhiW16HOwcMzCCbLzGrwnYuvOI7e0AfTEYfqBpqBvmTbC4k4KJNw1bCINgqcRhs/P8HlsM= ; 12, 25 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login) 12, 25 -- by smtp103.sbc.mail.mud.yahoo.com with SMTP; 5 Mar 2009 13:19:15 -0000 12, 25 -- X-YMail-OSG: mrmqHZAVM1me.BfX86abUjDAiDz8obaF6q.Tgui28IJ4tys0oCpo.HCBgo7vNHVr2WwvokaUc.lkamuv8cfRlqFVdtuxPIFuL2Z5lqLinA1ibtcMKwoONhtWtMxkjzNusnohA2APdPI9ZIsT.A_lL2e7bmTcOFQQQR6G0UJiIShmj6KN8a5obd5P68omwBWARZ7k1w3gNlX5SIJ23NSs_etiapLsM7E- 12, 25 -- X-Yahoo-Newman-Property: ymail-3 12, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 25 -- Date: Thu, 05 Mar 2009 07:14:54 -0600 12, 25 -- To: eikonika-at-otenet.gr, microscopy-at-microscopy.com 12, 25 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 12, 25 -- Subject: Re: [Microscopy] ESEM users, another sad story 12, 25 -- Cc: gerry-at-semtechsolutions.com 12, 25 -- In-Reply-To: {200903051056.n25AuvwO015036-at-ns.microscopy.com} 12, 25 -- References: {200903051056.n25AuvwO015036-at-ns.microscopy.com} 12, 25 -- Mime-Version: 1.0 12, 25 -- Content-Type: text/plain; charset="iso-8859-1" 12, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
You should be playing in Vegas instead of doing microscopy.
You are the only person I have heard of who uses Microsoft operating system and had never have to reboot a computer.
I must admit that we have all FEI microscopes and we are not free of computer problems.
We have an XL30 system that was upgraded last year from a DX4 configuration (originally Win3.1) to a system with two computers, one dedicated exclusively to control the SEM running Win2000, another one running our EDS system. The SEM computer is not connected to the internet, it communicates with the EDS PC via network. No user files or data are stored there nor any devices are allowed to be connected to it. Still we have to reboot the computer about once every two weeks or so. The second computer runs WinXP and is connected to the internet and users can store and transfer data to and from it. It also freezes couple of times each month.
Then we have a Tecnai12 TEM. It uses one computer (Win2000) running the TEM and two Gatan cameras, it too crashes from time to time. The worst part about this system is that the TEM vacuum system has to be restarted completely every time the computer reboots. Really bad design decision from FEI.
Also we have a CM300 TEM connected to another Win2000 computer. It does not run any TEM control software from FEI it only runs EDS and Gatan software. It also requires rebooting and I must say not significantly more frequently that the computers running FEI software. So my experience suggests it is Microsoft than anything else. We had the CM300 originally connected to a Macintosh which was running our EDS and Gatan software. We had to abandon it because EDAX and Gatan stopped support for Macintosh. During the two years that the system was running under the old Mac OS8 I did not have to reboot the system except when I did operating system upgrade or a new software installation on the Mac.
I wish I could have all our systems running and supported by MacOS, although It appears that since the adoption of Intel processors in the Macs or/and maybe the complexity of the new operating systems even Macs are not that reliable anymore.
Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
On Mar 5, 2009, at 12:34 AM, A.MARDINLY-at-numonyx.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ayten; } My opinion is that FEI tries to do too much with one computer. Our } 11 year old JEOL 2010F runs one computer (XP) dedicated to Gatan } Digital Micrograph (imaging and EELS), one computer (Win 2000) } dedicated to FASTEM, which runs the microscope, and one computer } (XP) to run the EDX system (Noran). Ethernet communicates between } the three to carry system information like magnification and imaging } mode. We NEVER have to reboot these computers and we NEVER have } system freezes while running the microscope. Plus, the microscope is } operated with knobs after just a few clicks to set it up. Am I just } lucky or what? } } John Mardinly, } Numonyx } } -----Original Message----- } X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] } Sent: Wednesday, March 04, 2009 11:40 PM } To: MARDINLY, A } Subject: [Microscopy] Operating system.. Re: Esem users } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The microscopes I have used so far had some computer interface and all } were using some version of Microsoft Windows operating system. } Unfortunately, all the systems have crashed from time to time due to } computer problems. } } In my opinion, Microsoft Windows OS needs to be rebooted periodically, } otherwise it is gonna crash in the middle of most important task. And, } you are gonna loose your best TEM images! } } I have also used Linux operating system for computational work. Linux } is much more stable compared to Windows. I wonder if electron } microscope vendors ever considered using something like Linux as } operating system. } } We have a new TEM from FEI and the imaging software has crashed a few } times already (this is making me nervous). The computer has only FEI } installed software; TEM server, imaging interface etc. No Internet } connection either. Hard drives are pretty much empty, as well. } } I have to give a break from observation of the sample and save my } images frequently. I guess this will be a problem when someone wants } to observe and record images of kinematical process e.g. phase change, } radiation damage in TEM. } } Kind Regards. } Ayten. } } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } =========================== } } } } On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Well, put me in the non-Luddite camp. Sure, I think you need knobs } } for "touchy-feely" operations like focus and stigmation, but most } } of the other things I'm happy to have under the control of the } } computer because then I can automate them if I have to. Macro- } } Express runs my SEM. Now it does take a little troubleshooting and } } testing to get everything to work right, but it's very liberating } } to hit one virtual button and have a whole slew of routine things } } done for you while doing something else. I easily outstrip the } } productivity I used to consider quite respectable back in the } } analog days. In my limited experience in other labs with computer } } controlled equipment, I've found a lot of their troubles stem from } } treating the dedicated computer on the equipment just like any } } other computer. One I saw was so crammed with Internet chat, games } } and screensaver programs the scope software barely had room to turn } } around, let alone run the scope. My first rule - keep the computer } } clean a! } nd! } } only install what you absolutely need - farm everything else out } } to normal desktops. Only install one new thing at a time, and run } } the scope software for quite awhile (a week or more) until you're } } absolutely sure it's not gumming up the works. With this strategy I } } have a crash or lockup of the system on average about once a year, } } usually because I'm doing something stupid that I should know } } better not to do at that particular time. My SEM just turned 10 } } years old last year, and I fully expect it to keep going for at } } least another 10 without any tremendous difficulty. } } } } Oh, and of course it does help to have a person around with some } } experience and interest in computers. If you hate the things to } } begin with, they're probably going to respond by hating you back. } } } } My 2 cents Canadian (~1.56 cents US), } } } } Jim } } } } -- } } } } James M. Ehrman } } Digital Microscopy Facility } } Mount Allison University } } 63B York St. } } Sackville, NB E4L 1G7 } } CANADA } } } } phone: 506-364-2519 } } fax: 506-364-2505 } } email: jehrman-at-mta.ca } } www: http://www.mta.ca/dmf } } } } } ==============================Original } Headers============================== } 12, 32 -- From celikaktas-at-gmail.com Thu Mar 5 01:33:09 2009 } 12, 32 -- Received: from fk-out-0910.google.com (fk- } out-0910.google.com [209.85.128.188]) } 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n257X8E3003823 } 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 01:33:08 } -0600 } 12, 32 -- Received: by fk-out-0910.google.com with SMTP id } z23so1377377fkz.2 } 12, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 04 Mar 2009 } 23:33:07 -0800 (PST) } 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 32 -- d=gmail.com; s=gamma; } 12, 32 -- h=domainkey-signature:mime- } version:received:date:message-id:subject } 12, 32 -- :from:to:content-type:content-transfer-encoding; } 12, 32 -- bh=AYThodq9LiLfTHZnje86wKZbYhgtEia/WM7U/C7u7eo=; } 12, 32 -- b=vpUgvbP6P1a+c3ADdQ3obERggm8/ } tBxXtmTbE4BtWljGYmJM7xBEsfC7oO4sW00YW3 } 12, 32 -- cwoDHZdHj+3dKsXE68fwbUGnf68tuj+vH } +7xHdquQ6ajvNkuxRW9bz5tveZlLnrDNihk } 12, 32 -- wSpgxm0W3AkpsAlUqdIbVa/6Pto+ujtFpTJm0= } 12, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 12, 32 -- d=gmail.com; s=gamma; } 12, 32 -- h=mime-version:date:message- } id:subject:from:to:content-type } 12, 32 -- :content-transfer-encoding; } 12, 32 -- b=JQOCZQ0bWfi/cjIj2AHy4LiaVIOvCi9t7ioessoijZ/ } 1MwmapyIDtWXubqnbmupaO9 } 12, 32 -- AbwDBjHcn/gEyD0ssuOy8oamVu2UFCJYtT0r1/ } RzBjXBeWt2boQrxSHs66rUtU6fTzbe } 12, 32 -- aWSrRRbT90N998YD1QvvNH8auLkyNUw5ir0u0= } 12, 32 -- MIME-Version: 1.0 } 12, 32 -- Received: by 10.103.219.17 with SMTP id w17mr396159muq. } 36.1236238387799; Wed, } 12, 32 -- 04 Mar 2009 23:33:07 -0800 (PST) } 12, 32 -- Date: Thu, 5 Mar 2009 10:33:07 +0300 } 12, 32 -- Message-ID: {1075c5c10903042333i63c8816fh641be80aa3a3a4f7-at-mail.gmail.com } } } 12, 32 -- Subject: Operating system.. Re: Esem users } 12, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} } 12, 32 -- To: microscopy {Microscopy-at-microscopy.com} } 12, 32 -- Content-Type: text/plain; charset=UTF-8 } 12, 32 -- Content-Transfer-Encoding: 8bit } 12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n257X8E3003823 } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 21, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 5 02:29:53 2009 } 21, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net } (mail1.numonyx.com [57.77.12.37]) } 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n258TrW8018802 } 21, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 5 Mar 2009 02:29:53 } -0600 } 21, 29 -- Received: from exdresfenmx01.numonyx.local (unknown } [10.96.252.22]) } 21, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with } ESMTP id 8D05D1440A2; } 21, 29 -- Thu, 5 Mar 2009 01:34:05 -0500 (EST) } 21, 29 -- Received: from EXDRESBENMX012.numonyx.local } ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft } SMTPSVC(6.0.3790.3959); } 21, 29 -- Thu, 5 Mar 2009 03:29:52 -0500 } 21, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 21, 29 -- Content-class: urn:content-classes:message } 21, 29 -- MIME-Version: 1.0 } 21, 29 -- Content-Type: text/plain; } 21, 29 -- charset="iso-8859-1" } 21, 29 -- Subject: RE: [Microscopy] Operating system.. Re: Esem users } 21, 29 -- Date: Thu, 5 Mar 2009 03:29:27 -0500 } 21, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9EE95D6-at-EXDRESBENMX012.numonyx.local } } } 21, 29 -- In-Reply-To: {200903050740.n257eMap015286-at-ns.microscopy.com} } 21, 29 -- X-MS-Has-Attach: } 21, 29 -- X-MS-TNEF-Correlator: } 21, 29 -- Thread-Topic: [Microscopy] Operating system.. Re: Esem users } 21, 29 -- Thread-Index: AcmdZauU4H31zME3SlyzDz5WmfaahgABTAcg } 21, 29 -- References: {200903050740.n257eMap015286-at-ns.microscopy.com} } 21, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} } 21, 29 -- To: {celikaktas-at-gmail.com} } 21, 29 -- Cc: {Microscopy-at-Microscopy.com} } 21, 29 -- X-OriginalArrivalTime: 05 Mar 2009 08:29:52.0130 (UTC) } FILETIME=[8E021620:01C99D6C] } 21, 29 -- Content-Transfer-Encoding: 8bit } 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n258TrW8018802 } ==============================End of - } Headers==============================
Hi Frank, You're right. The management types who have never actually done the work in their respective industries don't understand or appreciate the value of "professionals".
For many years I've told people that the SEM is NOT a microscope, despite the name. It is a signal generator and one or more signal processors. You tell me what you want to see and I can probably show it to you. Then we can have a long talk about what is real.
High levels of automation first started showing up in EDS systems. As most everyone on this Listserver is aware, there are so many variables involved in x-ray analysis that one MUST understand what is going on with the equipment and the sample. Using an auto-ident function almost always brings up a number of obviously incorrect element identifications. Most of us have heard at least one person say, "But the computer says..." My response is always, "I don't give a damn what the computer says."
This is now aggravated by the high level of automation in the SEMs and the "anyone can run this" attitude that not only disrespects the hard-earned expertise of professional microscopists, but has the potential to introduce huge amounts of essentially bogus data to both scientific and industrial data bases and even "expert systems".
Automation is a wonderful thing, provided you understand the underlying equipment, processes and theories. Yes, it is theoretically possible to create the Encyclopedia Britannica with a huge number of primates banging on keyboards, but it is much more efficient, and has a far higher likelihood of succeeding, if it is created by a small group of humans, expert in their respective fields. The "trouble" is that you have to pay them more than other primates.
We've seen what "financial experts" have done to the world's financial systems. How do we keep the "bean counters" from doing to same thing to all the other industries? How do we make them realize that their most expensive resource (labor) is the most expensive because it is the most valuable resource they have at their disposal?
Sorry about the rant. I feel better, though.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
Sent: Thursday, March 05, 2009 7:07 AM To: kenconverse-at-qualityimages.biz
Let me kick another thought into this discussion: most companies management wants computerized equipment. Why? They perceive then as less expensive to operate. No need for several experts at high salary and benefits, perhaps not even one. The tech rep sets it up and each user just walks up and uses it. Can't screw up the settings, cause the computer "remembers" the original settings.
I've see this with GC/MS, with IR, with image analysis equipment with TEMs and SEMs. Even the adds for this equipment suggest at "expert in a box" with their equipment.
now I'm not saying they are total to blame... I've heard sales pitches to management about justification for a piece of equipment by claiming the ease of operation in a multi-user environment: "blab blab..free standing, user friendly and we don't need dedicated operators....blab blab." I suspect we've even used this argument to get equipment we needed.
I needed a new EDS system at Degussa, I didn't select the best, I selected one that was the most user friendly 'cause I could justify it.
So sign me up for the Luddite Microscopy Group (humm..maybe a new Linked-In group), but I know to some small degree I'm responsible.
stay safe....... Frank Reformed Luddite Microscopist
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==============================Original Headers============================== 8, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 5 06:05:01 2009 8, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25C515R019105 8, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 06:05:01 -0600 8, 22 -- In-Reply-To: {200903051057.n25AvaOA016153-at-ns.microscopy.com} 8, 22 -- Subject: Re: other not so sad story 8, 22 -- To: Microscopy-at-microscopy.com 8, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 8, 22 -- Message-ID: {OFE15843D7.2A79266A-ON85257570.0040DCFA-85257570.00425949-at-lincolnelectric.c om} 8, 22 -- Date: Thu, 5 Mar 2009 07:04:42 -0500 8, 22 -- From: Frank_Karl-at-lincolnelectric.com 8, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM, 8, 22 -- CD-MIME complete at 03/05/2009 07:04:43 AM, 8, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM, 8, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 03/05/2009 07:04:43 AM, 8, 22 -- Serialize complete at 03/05/2009 07:04:43 AM 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: text/plain; 8, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 27, 26 -- From kenconverse-at-qualityimages.biz Thu Mar 5 08:36:47 2009 27, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25EaleX012662 27, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 08:36:47 -0600 27, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta06.mail.rr.com 27, 26 -- with ESMTP 27, 26 -- id {20090305143647.DRGZ3201.cdptpa-omta06.mail.rr.com-at-Ken} ; 27, 26 -- Thu, 5 Mar 2009 14:36:47 +0000 27, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 27, 26 -- To: {Frank_Karl-at-lincolnelectric.com} , 27, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 27, 26 -- Subject: RE: [Microscopy] Re: other not so sad story 27, 26 -- Date: Thu, 5 Mar 2009 09:36:40 -0500 27, 26 -- Message-ID: {D58AF4A5C85046AC8DF69AED190BCAB7-at-Ken} 27, 26 -- MIME-Version: 1.0 27, 26 -- Content-Type: text/plain; 27, 26 -- charset="us-ascii" 27, 26 -- X-Priority: 3 (Normal) 27, 26 -- X-MSMail-Priority: Normal 27, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 27, 26 -- Importance: Normal 27, 26 -- Thread-Index: Acmdiuwq5OJiRTcSRuufg7Yilj7KjgAEKRcw 27, 26 -- In-Reply-To: {200903051207.n25C7CKO023060-at-ns.microscopy.com} 27, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 27, 26 -- Content-Transfer-Encoding: 8bit 27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25EaleX012662 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: UPEI/AVC
Title-Subject: [Filtered] TEM digital camera
Question: Hello, We have H7500 with AMT XR 40 digital camera. Just recently we started to have problems viewing images captured by the camera. Half of the computer screen was black. It turned out that aluminum foil that covers the screen broke off in half. We have to buy a new screen (ouch, expensive). We used this system only for a bit more then two years and I was wondering if anybody had similar problem and what might be the cause of it. TIA Dorota
I wonder if the problem traces back to some of the earlier days of instrument control. I recall that programs were written to directly access memory location to transfer data and control the instrument. Those interfaces were not necessarily in the form of a well-written device driver. Programs sometimes got out of control and changed a byte or two where they shouldn't have and the system came crashing down.
I would like to think that things have gotten better and that the software is written more carefully or that maybe there are some safeguards in place. The number of crashes I experience has dropped a lot since the early 1980s. However, I still have some software with various issues. It is just not quite so dramatic.
I suppose this is the reason that computers are dedicated to each function. Let one computer control the microscope, another the EDS, and another the web surfing. If the EM control program goes awry, it won't take out my browser session - or vice versa.
Warren
-----Original Message----- X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] Sent: Thursday, March 05, 2009 1:34 AM To: wesaia-at-iastate.edu
The microscopes I have used so far had some computer interface and all were using some version of Microsoft Windows operating system. Unfortunately, all the systems have crashed from time to time due to computer problems.
In my opinion, Microsoft Windows OS needs to be rebooted periodically, otherwise it is gonna crash in the middle of most important task. And, you are gonna loose your best TEM images!
I have also used Linux operating system for computational work. Linux is much more stable compared to Windows. I wonder if electron microscope vendors ever considered using something like Linux as operating system.
We have a new TEM from FEI and the imaging software has crashed a few times already (this is making me nervous). The computer has only FEI installed software; TEM server, imaging interface etc. No Internet connection either. Hard drives are pretty much empty, as well.
I have to give a break from observation of the sample and save my images frequently. I guess this will be a problem when someone wants to observe and record images of kinematical process e.g. phase change, radiation damage in TEM.
Kind Regards. Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Wed, Mar 4, 2009 at 10:24 PM, {jehrman-at-mta.ca} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Well, put me in the non-Luddite camp. Sure, I think you need knobs for "touchy-feely" operations like focus and stigmation, but most of the other things I'm happy to have under the control of the computer because then I can automate them if I have to. Macro-Express runs my SEM. Now it does take a little troubleshooting and testing to get everything to work right, but it's very liberating to hit one virtual button and have a whole slew of routine things done for you while doing something else. I easily outstrip the productivity I used to consider quite respectable back in the analog days. In my limited experience in other labs with computer controlled equipment, I've found a lot of their troubles stem from treating the dedicated computer on the equipment just like any other computer. One I saw was so crammed with Internet chat, games and screensaver programs the scope software barely had room to turn around, let alone run the scope. My first rule - keep the computer clean a! nd! }  only install what you absolutely need - farm everything else out to normal desktops. Only install one new thing at a time, and run the scope software for quite awhile (a week or more) until you're absolutely sure it's not gumming up the works. With this strategy I have a crash or lockup of the system on average about once a year, usually because I'm doing something stupid that I should know better not to do at that particular time. My SEM just turned 10 years old last year, and I fully expect it to keep going for at least another 10 without any tremendous difficulty. } } Oh, and of course it does help to have a person around with some experience and interest in computers. If you hate the things to begin with, they're probably going to respond by hating you back. } } My 2 cents Canadian (~1.56 cents US), } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB  E4L 1G7 } CANADA } } phone: 506-364-2519 } fax:  506-364-2505 } email: jehrman-at-mta.ca } www:  http://www.mta.ca/dmf
==============================Original Headers============================== 21, 36 -- From wesaia-at-iastate.edu Thu Mar 5 11:09:23 2009 21, 36 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25H9LGd012726 21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:09:22 -0600 21, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 21, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n25H9KaB013662 21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:09:20 -0600 21, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 21, 36 -- id 1172_a03d9d7c_09a6_11de_b857_001372578af6; 21, 36 -- Thu, 05 Mar 2009 10:56:51 -0600 21, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 21, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n25H9H7h011739 21, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:09:18 -0600 21, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 21, 36 -- Thu, 5 Mar 2009 11:09:19 -0600 21, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 36 -- Content-class: urn:content-classes:message 21, 36 -- MIME-Version: 1.0 21, 36 -- Content-Type: text/plain; 21, 36 -- charset="iso-8859-1" 21, 36 -- Subject: RE: [Microscopy] Operating system.. Re: Esem users 21, 36 -- Date: Thu, 5 Mar 2009 11:09:41 -0600 21, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703B2A3C1-at-maire.eng.iastate.edu} 21, 36 -- In-Reply-To: {200903050733.n257Xupp004878-at-ns.microscopy.com} 21, 36 -- X-MS-Has-Attach: 21, 36 -- X-MS-TNEF-Correlator: 21, 36 -- Thread-Topic: [Microscopy] Operating system.. Re: Esem users 21, 36 -- Thread-Index: AcmdZL9bEe/2u+hdS2ardWh/V4LUSAAT2u7A 21, 36 -- References: {200903050733.n257Xupp004878-at-ns.microscopy.com} 21, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 21, 36 -- To: {Microscopy-at-microscopy.com} 21, 36 -- X-OriginalArrivalTime: 05 Mar 2009 17:09:19.0886 (UTC) FILETIME=[1F703AE0:01C99DB5] 21, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.5.165527 21, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_5000_5999 0, BODY_SIZE_7000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_AGE_BODY 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NUM 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 21, 36 -- Content-Transfer-Encoding: 8bit 21, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25H9LGd012726 ==============================End of - Headers==============================
I thought I would throw my thoughts into the ring here since this seems to be an issue with everyone. I have a unique perspective on the FEI systems as I was working as a Field Service Engineer up until last summer. I'm now sitting in your seats operating an FEI system. I have a FIB820 which runs on Windows 3.11 for WorkGroups. In the past few months using this system I've found this computer and Windows 3.11 need rebooting every week. Without the reboot the system tends to have UI system errors and slows down considerably. This system though is not upgradable and there are no PC spares. So once the PC dies I'll be searching far and wide for something that runs Win 3.11, or some upgrade from another company so I can be back up and running.
With regards to the new systems though. In the field I found that the problem wasn't generally with FEI's software. The problem usually was with the PC's that were used. Most of the newer systems we're using HP PC's. These seemed to have motherboard problems which would cause re-booting, software errors, slowdowns, and lockups. Once the PC was replaced everything was fine. Most newer systems were using XP, and some on 2000. These were mainly on the Quanta and Nova systems. Also the operators inadvertently tended to cause some of their own problems by changing settings and loading other software.
The older systems such as the 235's, 800's, and 1200's all used Industrial PC's mainly now the Advantec (Black Box). These ran everything from Win 3.1 to XP and made systems upgradable as long as the software was made to work with the system and in some cases EPROMS and communications boards upgraded. Again the problems encountered were generally related to Windows and misc. PC problems and not the FEI software. Sometimes on these the SCSI system can cause reboots and lockups.
XL series and systems like the 820 all use "different" PC's. The components and the Windows software seem to vary greatly. Some can be upgraded to Win2000 and others like mine seem to be stuck at whatever they're running. The good news is that these PC's seem to be better built then the newer ones as this one on my 820 has been running for 10+ years.
As for systems running Linux/Unix. Applied materials SemVision systems did use a Unix based system and would have been very stable if the AMAT user software had been better. The world would be a better place if run on Linux or Mac's before the Intel days.
We also have an Hitachi SEM here which has excellent resolution but the UI and the Hardware are not user friendly or very well designed when compared to FEI's systems. At least in my opinion, which obviously is tilted toward FEI. It does use Windows 2000/XP shell at least and seems to be stable enough that reboots are very few.
FEI systems and software to me are still the best out there though. The biggest problem I see is Microsoft and PC's, along with each manufacturers method of communicating with Hardware. If quality PC's were used along with Windows XP and drivers/software that was well written and debugged I don't think any of these systems would have need of any more rebooting then each of our Desktop PC's running XP. The WinXP PC I'm typing this is on now runs worse then the FIB820's Win3.11 PC and requires rebooting regularly. So consider how much you reboot your Home or Office PC and compare that to your microscope.
==============================Original Headers============================== 8, 32 -- From ian.drucker-at-gmail.com Thu Mar 5 11:43:40 2009 8, 32 -- Received: from mail-fx0-f174.google.com (mail-fx0-f174.google.com [209.85.220.174]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25HhcE1027186 8, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:43:39 -0600 8, 32 -- Received: by fxm22 with SMTP id 22so28706fxm.18 8, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 05 Mar 2009 09:43:36 -0800 (PST) 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 32 -- d=gmail.com; s=gamma; 8, 32 -- h=domainkey-signature:mime-version:received:date:message-id:subject 8, 32 -- :from:to:content-type:content-transfer-encoding; 8, 32 -- bh=p7cRkFdLmSxFt8oaJCs03AVMcFal7dewkTwJgsVzXLo=; 8, 32 -- b=lF4I30zETzMnu1bo6A1gAo7jhZR3N6ZKntx+IdgRONoopEosImQf9tTTP+e4Urmh80 8, 32 -- bUQ7KmqNNUag+KFVrRb+h2av7zHq8VWcIF9vYba0+N7FoI5rlgqfSa/2yKZ0ZOzqMIgR 8, 32 -- Q+yMxJ8hmZx6ZPS+ISObFLNj5ylFom5CbH0Qw= 8, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 8, 32 -- d=gmail.com; s=gamma; 8, 32 -- h=mime-version:date:message-id:subject:from:to:content-type 8, 32 -- :content-transfer-encoding; 8, 32 -- b=vXD6lcoG/LeA0HCp9oV+Ho9OZV/T8oqFSHalI3HJ46fDETVPYR7BL6BzrKzxT1QjbT 8, 32 -- cfBHQOYCRcvLecHQz/DfMiQNm4YCwJaUPITiFMDIsODhZopQHGK4bjW/Fyt6Rq7jjBvW 8, 32 -- 2Ei7EFqtILeEzIinTRJlNpwFBaNDK4XTvbn90= 8, 32 -- MIME-Version: 1.0 8, 32 -- Received: by 10.103.246.1 with SMTP id y1mr648585mur.116.1236275016435; Thu, 8, 32 -- 05 Mar 2009 09:43:36 -0800 (PST) 8, 32 -- Date: Thu, 5 Mar 2009 10:43:36 -0700 8, 32 -- Message-ID: {c0bfda950903050943r4e21489avb3db134e0bae4911-at-mail.gmail.com} 8, 32 -- Subject: Regarding FEI Computers and Software 8, 32 -- From: ID {ian.drucker-at-gmail.com} 8, 32 -- To: Microscopy-at-microscopy.com 8, 32 -- Content-Type: text/plain; charset=ISO-8859-1 8, 32 -- Content-Transfer-Encoding: 8bit 8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25HhcE1027186 ==============================End of - Headers==============================
Here's the perspective from an engineer who is in a position to know what he's speaking about, and who shall remain unnamed. I thought this was very informative.
"Hi Phil and Randy,
.......... let me explain what's going on. For quite a while now, there have been calculations required to make your "turning a knob" into a predictable and usable result in, say, the magnification. With current microscopes, the optics model is quite intricate. Customers (especially the ones new to electron microscopy) don't want to "control the objective lens current" - they want to "set the magnification". Even the old Philips CMs had computers inside ("CM" stood for "Computerized Microscope"). So even when you don't _see_ a computer (as in a beige box next to the microscope), there certainly is one.
Given the enormous pace at which consumer-level computer hardware is progressing, it simply doesn't make sense to try and build your own internal computer systems anymore. Customers expect more and more from their systems (perhaps you personally don't, but especially where microscopes are used for routine/QA work, people demand that they can be automated).
The downside of all this is that consumer PC hardware has a "few years" time scale whereas microscopes have a "several decades" time scale. At the time, using ISA slots was a good idea because ISA "wasn't going anywhere soon"!. Well, it went somewhere anyway, and systems which still have ISA slots are getting rare.
Microscope manufacturers are obviously trying to minimize these risks now. We won't jump on just any new technology lest it becomes an "also ran" so we want a proven track record, but that means that probably half of this technologies life span has already passed once we decide we can safely use it. We are caught between a rock and a hard place.
But we are learning. The obvious advantages to riding the "commodity hardware" wave cannot be ignored, and on the other hand we started to realize that when the PC world says "this new technology/bus/OS will be here forever" they really mean "at least until next week!". So we have to tread carefully, and that's what we're doing.
I just wanted to let you know that we engineers don't sit around waiting anxiously for some PC technology to go obsolete so we finally have something to do again :-)"
Signed, Joe the Engineer.
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 14, 27 -- From TindallR-at-missouri.edu Thu Mar 5 12:22:16 2009 14, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25IMETb009662 14, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 12:22:15 -0600 14, 27 -- X-IronPort-Anti-Spam-Filtered: true 14, 27 -- X-IronPort-Anti-Spam-Result: ApoEANumr0nRauUo/2dsb2JhbADJJQEJhnyITYJXgTEG 14, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 14, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Mar 2009 12:22:11 -0600 14, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 27 -- Thu, 5 Mar 2009 12:22:11 -0600 14, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 27 -- Content-class: urn:content-classes:message 14, 27 -- MIME-Version: 1.0 14, 27 -- Content-Type: text/plain; 14, 27 -- charset="us-ascii" 14, 27 -- Subject: EMs and computers 14, 27 -- Date: Thu, 5 Mar 2009 12:22:07 -0600 14, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7EC3-at-UM-XMAIL08.um.umsystem.edu} 14, 27 -- X-MS-Has-Attach: 14, 27 -- X-MS-TNEF-Correlator: 14, 27 -- Thread-Topic: EMs and computers 14, 27 -- Thread-Index: Acmdv0p3nFlf+p31Qv2GdkeNmnxEVw== 14, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 14, 27 -- To: {microscopy-at-microscopy.com} 14, 27 -- X-OriginalArrivalTime: 05 Mar 2009 18:22:11.0914 (UTC) FILETIME=[4D5EC2A0:01C99DBF] 14, 27 -- Content-Transfer-Encoding: 8bit 14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25IMETb009662 ==============================End of - Headers==============================
On Mar 4, 2009, at 1:27 PM, vray-at-partbeamsystech.com wrote:
} But it is probably easier to develop "feel" for inherently "manual" } operations, like alignments or stigmation for example, by turning } knobs } (even though automated focus/stig works most of the times on most of } the } tools).
Dear Valery, et al., I advise being careful about the automated functions. Like most things, it all depends on what you need to do, whether the autofunctions are adequate. I wrote a piece in Microscopy Today a few years ago now about this. I found that auto focus, in particular, has a tendency to over-correct, and if you re-do the autofocus, the focus value found will typically oscillate with decreasing amplitude until it reaches a stable value. Thus, if all you need is a pretty well focussed image--one that looks good, or one where you will set defocus to several micrometers--autofocus works well, and it is quick, convenient, and exposes your specimen to minimal extra radiation. On the other hand, if you are doing single-particle cryoEM at high mag and high resolution, for example, it is good to check by looking at a live FFT to see whether autofocus has in fact found true focus from which you can set a relatively small defocus. (Of course, in this case you'll be doing this at a position offset from your specimen to avoid radiation damage.) Furthermore, it is pretty simple to focus and stigmate quickly with live FFT at mags of ~100 kx. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Mar 5 13:13:34 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25JDX4v024920 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 13:13:34 -0600 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id C4CF966E3D4E 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:31 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id C489766E206C 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:30 -0800 (PST) 6, 22 -- Message-Id: {88E1DA52-2521-4ACD-8818-674B1D3A1797-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200903042127.n24LRWSS023015-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] Esem users 6, 22 -- Date: Thu, 5 Mar 2009 11:13:30 -0800 6, 22 -- References: {200903042127.n24LRWSS023015-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I think that there should be a separation in which the microscope has "embedded control" (whether or not it is truly embedded or an external "box") that controls the microscope functions. This box should not be a concern to the purchaser/user of the microscope, and could be upgraded as needed by the manufacturer to properly do the job that it was originally delivered to do, or provide any enhancements possible. It should provide basic control functions and accept external commands for microscope control; it would interface to actual knobs or external digital interfaces that it is documented to support - there are many that could be used. It should be extremely reliable, and the manufacturers can't blame the OS since we don't care what is inside their box as long as it works. The "box" in this context is part of the microscope and the manufacturer should be obligated to maintain it's functionality for a stated period; I believe that JEOL has a policy of maintaining a machine as long as it is kept under contract and they have done an admirable job on our older scopes.
The client side computer can then be whatever the client requires and can be updated as needed for new storage, graphics, networks, etc and either the manufacturer, second sources, or open sources can provide the control program. Purchasers drive the market and could insist on open standards. In my opinion, government funds should not be allowed to be spent on equipment lacking complete documentation. Manufacturers will say - "if we did that all hell would break loose", but that is just cover; based on what we see on this list there are plenty of issues with the manufacturers in control. For robustness, would you choose Linux or a popular commercial OS to control your flight to the moon? I think I would go with Linux and well-vetted open sources :-)
Dale
TindallR-at-missouri.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Here's the perspective from an engineer who is in a position to know } what he's speaking about, and who shall remain unnamed. I thought this } was very informative. } } "Hi Phil and Randy, } } .......... let me explain what's going on. For quite a while now, there } have been calculations required to make your "turning a knob" into a } predictable and usable result in, say, the magnification. With current } microscopes, the optics model is quite intricate. } Customers (especially the ones new to electron microscopy) don't want to } "control the objective lens current" - they want to "set the } magnification". Even the old Philips CMs had computers inside ("CM" } stood for "Computerized Microscope"). So even when you don't _see_ a } computer (as in a beige box next to the microscope), there certainly is } one. } } Given the enormous pace at which consumer-level computer hardware is } progressing, it simply doesn't make sense to try and build your own } internal computer systems anymore. Customers expect more and more from } their systems (perhaps you personally don't, but especially where } microscopes are used for routine/QA work, people demand that they can be } automated). } } The downside of all this is that consumer PC hardware has a "few years" } time scale whereas microscopes have a "several decades" time scale. At } the time, using ISA slots was a good idea because ISA "wasn't going } anywhere soon"!. Well, it went somewhere anyway, and systems which } still have ISA slots are getting rare. } } Microscope manufacturers are obviously trying to minimize these risks } now. We won't jump on just any new technology lest it becomes an "also } ran" so we want a proven track record, but that means that probably half } of this technologies life span has already passed once we decide we can } safely use it. We are caught between a rock and a hard place. } } But we are learning. The obvious advantages to riding the "commodity } hardware" wave cannot be ignored, and on the other hand we started to } realize that when the PC world says "this new technology/bus/OS will be } here forever" they really mean "at least until next week!". So we have } to tread carefully, and that's what we're doing. } } I just wanted to let you know that we engineers don't sit around waiting } anxiously for some PC technology to go obsolete so we finally have } something to do again :-)" } } Signed, Joe the Engineer. } } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } } ==============================Original Headers============================== } 14, 27 -- From TindallR-at-missouri.edu Thu Mar 5 12:22:16 2009 } 14, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25IMETb009662 } 14, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 12:22:15 -0600 } 14, 27 -- X-IronPort-Anti-Spam-Filtered: true } 14, 27 -- X-IronPort-Anti-Spam-Result: ApoEANumr0nRauUo/2dsb2JhbADJJQEJhnyITYJXgTEG } 14, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) } 14, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 05 Mar 2009 12:22:11 -0600 } 14, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 14, 27 -- Thu, 5 Mar 2009 12:22:11 -0600 } 14, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 14, 27 -- Content-class: urn:content-classes:message } 14, 27 -- MIME-Version: 1.0 } 14, 27 -- Content-Type: text/plain; } 14, 27 -- charset="us-ascii" } 14, 27 -- Subject: EMs and computers } 14, 27 -- Date: Thu, 5 Mar 2009 12:22:07 -0600 } 14, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7EC3-at-UM-XMAIL08.um.umsystem.edu} } 14, 27 -- X-MS-Has-Attach: } 14, 27 -- X-MS-TNEF-Correlator: } 14, 27 -- Thread-Topic: EMs and computers } 14, 27 -- Thread-Index: Acmdv0p3nFlf+p31Qv2GdkeNmnxEVw== } 14, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 14, 27 -- To: {microscopy-at-microscopy.com} } 14, 27 -- X-OriginalArrivalTime: 05 Mar 2009 18:22:11.0914 (UTC) FILETIME=[4D5EC2A0:01C99DBF] } 14, 27 -- Content-Transfer-Encoding: 8bit } 14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n25IMETb009662 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 22 -- From dac-at-research.umass.edu Thu Mar 5 14:18:16 2009 5, 22 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25KIEtq008485 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 14:18:15 -0600 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 5, 22 -- (authenticated bits=0) 5, 22 -- by race5.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n25KIANe005757 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 15:18:11 -0500 5, 22 -- Message-ID: {49B0340E.8040609-at-research.umass.edu} 5, 22 -- Date: Thu, 05 Mar 2009 15:20:30 -0500 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 22 -- Reply-To: dac-at-research.umass.edu 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 5, 22 -- MIME-Version: 1.0 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] EMs and computers 5, 22 -- References: {200903051834.n25IY0TH018019-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200903051834.n25IY0TH018019-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
maybe not Luddites but perhaps "Grumpy Old Microscopists" - not in any ageist way of course. But then who would we be affiliated with: 1 MSA 2 Grumpy Old Men/Women - I assume that this form of entertainment from the likes of Rik Wakeman is available in the US and elsewhere.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Frank_Karl-at-lincolnelectric.com
Dear Bill,
I could not agree more with your "cautionary note" - this is exactly why I said that automated functions work well "most of the times on most of the tools"; it should probably be added "with most of the samples" :)
As was already pointed out in this thread, when SEMs are used for routine QA or process control, or FIBs for repetitive cutting of dozens of FA samples, demand for push-button operation and automation is very large and justified; in research applications flexibility and ultimate performance take priority.
My argument is that "knobs" should not be disappearing with integration of GUI and automated functions, but remain available (at least) as user-specifiable option, even though they are working through the computer_we_do_not_see
Cheers, Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Thursday, March 05, 2009 2:16 PM To: vray-at-partbeamsystech.com
On Mar 4, 2009, at 1:27 PM, vray-at-partbeamsystech.com wrote:
} But it is probably easier to develop "feel" for inherently "manual" } operations, like alignments or stigmation for example, by turning } knobs } (even though automated focus/stig works most of the times on most of } the } tools).
Dear Valery, et al., I advise being careful about the automated functions. Like most things, it all depends on what you need to do, whether the autofunctions are adequate. I wrote a piece in Microscopy Today a few years ago now about this. I found that auto focus, in particular, has a tendency to over-correct, and if you re-do the autofocus, the focus value found will typically oscillate with decreasing amplitude until it reaches a stable value. Thus, if all you need is a pretty well focussed image--one that looks good, or one where you will set defocus to several micrometers--autofocus works well, and it is quick, convenient, and exposes your specimen to minimal extra radiation. On the other hand, if you are doing single-particle cryoEM at high mag and high resolution, for example, it is good to check by looking at a live FFT to see whether autofocus has in fact found true focus from which you can set a relatively small defocus. (Of course, in this case you'll be doing this at a position offset from your specimen to avoid radiation damage.) Furthermore, it is pretty simple to focus and stigmate quickly with live FFT at mags of ~100 kx. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Mar 5 13:13:34 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n25JDX4v024920 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 13:13:34 -0600 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id C4CF966E3D4E 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:31 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id C489766E206C 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 11:13:30 -0800 (PST) 6, 22 -- Message-Id: {88E1DA52-2521-4ACD-8818-674B1D3A1797-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200903042127.n24LRWSS023015-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] Esem users 6, 22 -- Date: Thu, 5 Mar 2009 11:13:30 -0800 6, 22 -- References: {200903042127.n24LRWSS023015-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 17, 25 -- From vray-at-partbeamsystech.com Thu Mar 5 14:50:29 2009 17, 25 -- Received: from smtp104.biz.mail.re2.yahoo.com (smtp104.biz.mail.re2.yahoo.com [206.190.52.173]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n25KoSYN004104 17, 25 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 14:50:29 -0600 17, 25 -- Message-Id: {200903052050.n25KoSYN004104-at-ns.microscopy.com} 17, 25 -- Received: (qmail 30519 invoked from network); 5 Mar 2009 20:50:26 -0000 17, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.67.14.77 with login) 17, 25 -- by smtp104.biz.mail.re2.yahoo.com with SMTP; 5 Mar 2009 20:50:26 -0000 17, 25 -- X-YMail-OSG: by.0uukVM1nHe0V.0FeP8H6sEYBnwwQGvoL.kJ_x3dRjSyTOXm8X6byhKQNbD3E0dsg6D24H9CuYQ404RYAEEePwTUQwUK.J671uwdBsJlr.koul4qnXlQiqXxOgsc0kwcVQc9vWyrI7u6XoRIcWbfsetFCn5EK7p4SW.LlktQyo3cPtljLlwaRtRWOSgYcCoAagUt7iHozplG7g0hEw7BMSm3PmwVV2 17, 25 -- X-Yahoo-Newman-Property: ymail-3 17, 25 -- Reply-To: {vray-at-partbeamsystech.com} 17, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 17, 25 -- To: {tivol-at-caltech.edu} 17, 25 -- Cc: {microscopy-at-microscopy.com} 17, 25 -- Subject: RE: [Microscopy] Re: Esem users 17, 25 -- Date: Thu, 5 Mar 2009 15:53:23 -0500 17, 25 -- Organization: PBST / MEO Engineering 17, 25 -- MIME-Version: 1.0 17, 25 -- Content-Type: text/plain; 17, 25 -- charset="us-ascii" 17, 25 -- Content-Transfer-Encoding: 7bit 17, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 25 -- Thread-Index: Acmdxsa7P0PaslBaRNCiFiIe/2HKPAACPotg 17, 25 -- In-Reply-To: {200903051915.n25JFc9m026521-at-ns.microscopy.com} 17, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
As an electrical engineer, software coder, microcircuit designer and fixer, reverse engineerer, FA, and oh, a SEM user/operator for about ten years, here are my 5mS neuron works and 15 minutes of carpal tunnel typing.
I had three different flavors of Amray SEMs, starting with their 1610T, then their 1830 and lastly, their 1910FE. These were really reliable workhorses, IMO. The 1610T had a lot of knobs. The others had a few knobs and an assortment of buttons. The knobs and buttons were read by an embedded microcontroller (MCU). On some boards, each one had a MCU. In addition, the later models had multiple A/D and D/A converters which were controlled by an MCU. Thus, a knob on the panel is actually a potentiometer (pot) that is converted to digital via an A/D converter. Then, the MCU makes computations to do what the operator wants and sends new control bytes/commands to a D/A converter that changes mag, stig, focus, etc. So in circa 1985, computerization was already happening but inside the tool. KLA-Tencor killed Amray, to their recent dismay.
So at some point in time, most all SEMs and probably TEMs had one or more computers in the loop, but they were inside the tool rather than desktop or outside the tool. On the later Amrays, PC10 was the killer. This is the frame grabber and buffer that processed the SE signal and converted it to NTSC video. I have never seen a schematic for this board. But, as packed as it was with ICs, it was very reliable...thankfully. The 1830 and onwards added PC control via Nibblenet and I used Soft-Imaging ADDA-II for frame grabbing at 640x480 pixels and direct scan capture up to 4Kx4K. The EDAX Genesis image capture would do over 6K pixels. Very good. All went to TIF files.
I've been involved with de-integrating an FEI Sirion SFEG to remove the integrated EDAX EDS system. Also, dumping the SEM's Acer PC running WinNT. As I recall, NT was written by the author of DEC's VAX/VMS--a fabulous hardware and software architecture. Gone but fondly remembered. NT was the first Microsoft OS to feature virtual memory--something that Unix always had. Plug and play came into being with WinXP (forget about Vista, IMO). The progression from Win 3 to 95 to 95SE to NT to XP and now Vista is a long side discussion. The differences are subtle and unique and also of impact. This has been pointed out in prior postings.
It was also pointed out that a common interface between the PC and the tool's guts would be beneficial. Most useful interfaces are SCSI. This has been a standard for many years. It is problematic for several reasons. One is that early ISA cards worked one way (narrow) while newer versions (PCI) work multiple ways (SE, LVDT, et al.). This is a hassle to set up. But once done, it should not be an issue.
In the recent past, LEO (now Zeiss) did a nice job of PC control of the SEM. Around 2004, Zeiss offered a "hard panel" option. This was very good. If you like knobs and buttons you get them along with using the mouse and a PC GUI. Their GUI has always been very nice and easy to use. The only standard caution is to wait for a reasonably stable version of the GUI before adopting it. Programming is the process of putting bugs in software. The user is the one who finds the existence of bugs and the programmers eventually fix the bugs. Often, the bug fixes just introduce new bugs. Perhaps that is job security for programmers?
LEO/Zeiss failed on the implementation of 16-bit TIFF. I finally figured this out with the help of Adobe who was the holder of the TIFF standard at the time. Zeiss fixed the faulty byte swap order. Thank you.
Another nice feature that Zeiss has (I don't know if this is unique to them or not) is the ability to write versatile macros to automate the use of virtually all functions of the SEM. This is very handy. I recall from part of the discussion that at least one other maker might have a similar feature. But AFIK, the Zeiss macros are very powerful.
It is inevitable that technology is going to move forward. New SEMs will eventually become obsolete and/or unsupported or unsupportable. At some point, E-beam SEMs will be obsoleted by ion beam imaging tools for true nano-scale work. Even so, with tools becoming increasingly more complex behind the panels, it takes longer for the tools to stabilize and be readily usable. I don't recall that being the case for earlier generations of SEMs or FIBs.
Sorry about the novel-length posting. Perhaps this could be a side bar topic for discussion at M&M 2009? Wow, it sounds like there is a lot of frustration out there. However, I get the feeling that all of the company's service techs are good if not great. I second this. Zeiss and FEI have been very good. I have no experience with the others.
Final note--I only reboot Zeiss and EDAX PCs when I download Microsoft new updates to WinXP that requires re-boot. Otherwise, no reboot. I have Mozilla on both PCs. No big deal.
DISCLAIMER: I have no financial interest in any SEM tool maker. I just hope that what I have will work into the future and be supported. Zeiss says that any tool will be supported for ten years after discontinuation of production. EDAX support has been outstanding. Given the advancement of technology obsolescence, I think it is going to get worse overall--and this is not the fault of the makers. I was able to switch from Si(Li) to SDD and do not look back. In a few years under contract, EDAX will swap out the HP XW5000 PC for a new unit. This has been a good PC. I would suggest to all that any new PCs are at least RAID 1 using SATA drives.
Gary Gaugler, Ph.D.
==============================Original Headers============================== 16, 17 -- From gary-at-gaugler.com Thu Mar 5 22:40:03 2009 16, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 16, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n264e3pv029664 16, 17 -- for {microscopy-at-microscopy.com} ; Thu, 5 Mar 2009 22:40:03 -0600 16, 17 -- Message-Id: {200903060440.n264e3pv029664-at-ns.microscopy.com} 16, 17 -- Received: (qmail 11163 invoked from network); 5 Mar 2009 20:39:25 -0800 16, 17 -- Received: by simscan 1.1.0 ppid: 11160, pid: 11161, t: 0.1479s 16, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 16, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 16, 17 -- by smtp2 with SMTP; 5 Mar 2009 20:39:24 -0800 16, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 17 -- Date: Thu, 05 Mar 2009 20:39:54 -0800 16, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 16, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 16, 17 -- Subject: EMs and PCs 16, 17 -- Mime-Version: 1.0 16, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Dear Group - thank you to all who responded regarding the manual. We finally found a manual stuck away hidden in a drawer. Thanks again. Sincerely, Barbara
==============================Original Headers============================== 1, 17 -- From maloneyb-at-fiu.edu Fri Mar 6 05:39:46 2009 1, 17 -- Received: from fmailhost04.isp.att.net (fmailhost04.isp.att.net [207.115.11.54]) 1, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Bdk88022395 1, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 05:39:46 -0600 1, 17 -- Received: from [10.0.0.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 17 -- by isp.att.net (frfwmhc04) with ESMTP 1, 17 -- id {20090306113945H0400pti59e} ; Fri, 6 Mar 2009 11:39:45 +0000 1, 17 -- X-Originating-IP: [74.166.189.137] 1, 17 -- Message-ID: {49B10B81.8030808-at-fiu.edu} 1, 17 -- Date: Fri, 06 Mar 2009 06:39:45 -0500 1, 17 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 17 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 1, 17 -- MIME-Version: 1.0 1, 17 -- To: Microscopy-at-microscopy.com 1, 17 -- Subject: Manual found for ISI DS130 1, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am working with a student who is investigating plant cuticles, a coating on the outermost layer of cells that consists of the polymer cutin and waxes. When examining this feature with TEM the waxes are often lost. From what she has read, this occurs mainly during the resin polymerization stage. The waxes typically melt at 52-56° C. Currently she is using Spurrs resin, which has been recommended in the literature for the type of plants (mosses) that she is examining.
Possible approaches we are considering are:
1. Low temperature embedding using UV polymerization. We have equipment (Leica AFS) and experience with low temp embedding in Lowicryl HM-20 for immuno, but not for preserving waxes.
2. Microwave embedding in any resin. We have a Pella microwave with Coldspot, but haven't done much with plant tissue so far.
3. CryoSEM. We have a good FESEM, but do NOT have a cold stage, but would like to know whether anyone in this area (CT, MA, RI, NY) who does and would be able to help her with a one-time examination of her samples.
If anyone has used these or other techniques for preserving waxy cuticles or a similar material, we would be very interested in hearing about your experience.
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 10, 17 -- From MARIE.CANTINO-at-uconn.edu Fri Mar 6 08:05:17 2009 10, 17 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26E5GPg007821 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:05:17 -0600 10, 17 -- Received: from d46h165.public.uconn.edu (d46h165.public.uconn.edu [137.99.46.165]) 10, 17 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n26E5F7l020707 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 09:05:16 -0500 10, 17 -- Message-Id: {FAF6C351-B2CD-4052-853A-5822964648A6-at-uconn.edu} 10, 17 -- From: Marie Cantino {MARIE.CANTINO-at-uconn.edu} 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 10, 17 -- Mime-Version: 1.0 (Apple Message framework v929.2) 10, 17 -- Subject: EM preservation of waxy plant cuticles 10, 17 -- Date: Fri, 6 Mar 2009 09:05:15 -0500 10, 17 -- X-Mailer: Apple Mail (2.929.2) 10, 17 -- Content-Transfer-Encoding: 8bit 10, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26E5GPg007821 ==============================End of - Headers==============================
Try the paper below. I would use the SEM to look at an air dried sample first. My motto - always start with the easiest method!
Dave
Sample preparation for scanning electron microscopy of plant surfaces-Horses for courses A.K. Pathan a,*, J. Bond b, R.E. Gaskin a Micron 39 (2008) 1049-1061
-----Original Message----- X-from: MARIE.CANTINO-at-uconn.edu [mailto:MARIE.CANTINO-at-uconn.edu] Sent: 06 March 2009 14:13 To: David Patton
I am working with a student who is investigating plant cuticles, a coating on the outermost layer of cells that consists of the polymer cutin and waxes. When examining this feature with TEM the waxes are often lost. From what she has read, this occurs mainly during the resin polymerization stage. The waxes typically melt at 52-56° C. Currently she is using Spurrs resin, which has been recommended in the literature for the type of plants (mosses) that she is examining.
Possible approaches we are considering are:
1. Low temperature embedding using UV polymerization. We have equipment (Leica AFS) and experience with low temp embedding in Lowicryl HM-20 for immuno, but not for preserving waxes.
2. Microwave embedding in any resin. We have a Pella microwave with Coldspot, but haven't done much with plant tissue so far.
3. CryoSEM. We have a good FESEM, but do NOT have a cold stage, but would like to know whether anyone in this area (CT, MA, RI, NY) who does and would be able to help her with a one-time examination of her samples.
If anyone has used these or other techniques for preserving waxy cuticles or a similar material, we would be very interested in hearing about your experience.
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 10, 17 -- From MARIE.CANTINO-at-uconn.edu Fri Mar 6 08:05:17 2009 10, 17 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26E5GPg007821 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:05:17 -0600 10, 17 -- Received: from d46h165.public.uconn.edu (d46h165.public.uconn.edu [137.99.46.165]) 10, 17 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n26E5F7l020707 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 09:05:16 -0500 10, 17 -- Message-Id: {FAF6C351-B2CD-4052-853A-5822964648A6-at-uconn.edu} 10, 17 -- From: Marie Cantino {MARIE.CANTINO-at-uconn.edu} 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 10, 17 -- Mime-Version: 1.0 (Apple Message framework v929.2) 10, 17 -- Subject: EM preservation of waxy plant cuticles 10, 17 -- Date: Fri, 6 Mar 2009 09:05:15 -0500 10, 17 -- X-Mailer: Apple Mail (2.929.2) 10, 17 -- Content-Transfer-Encoding: 8bit 10, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26E5GPg007821 ==============================End of - Headers==============================
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==============================Original Headers============================== 23, 35 -- From David.Patton-at-uwe.ac.uk Fri Mar 6 08:35:24 2009 23, 35 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65]) 23, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n26EZMgj022519 23, 35 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:35:23 -0600 23, 35 -- Received: from (unknown [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp 23, 35 -- id 6476_0585c730_0a5c_11de_9f1e_00142221cca9; 23, 35 -- Fri, 06 Mar 2009 14:35:20 +0000 23, 35 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 23, 35 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 23, 35 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 23, 35 -- 2005)) with SMTP id {0KG300CFL9UPVS-at-mta02.uwe.ac.uk} for 23, 35 -- microscopy-at-microscopy.com; Fri, 06 Mar 2009 14:35:21 +0000 (GMT) 23, 35 -- Date: Fri, 06 Mar 2009 14:33:10 +0000 23, 35 -- From: David Patton {David.Patton-at-uwe.ac.uk} 23, 35 -- Subject: RE: [Microscopy] EM preservation of waxy plant cuticles 23, 35 -- In-reply-to: {200903061413.n26EDN05013763-at-ns.microscopy.com} 23, 35 -- To: MARIE.CANTINO-at-uconn.edu 23, 35 -- Message-id: {F247F674896BE243AD8263C5280E2BDB048C4CA7-at-egen-uwe01} 23, 35 -- MIME-version: 1.0 23, 35 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 23, 35 -- Content-type: text/plain; 23, 35 -- charset="utf-8" 23, 35 -- Content-class: urn:content-classes:message 23, 35 -- Thread-topic: [Microscopy] EM preservation of waxy plant cuticles 23, 35 -- Thread-index: AcmeZbjWsRUIl4KMR/mk7/B4NLA06AAAntIA 23, 35 -- X-MS-Has-Attach: 23, 35 -- X-MS-TNEF-Correlator: 23, 35 -- References: {200903061413.n26EDN05013763-at-ns.microscopy.com} 23, 35 -- X-NAIMIME-Disclaimer: 1 23, 35 -- X-NAIMIME-Modified: 1 23, 35 -- X-NAI-Spam-Score: 0 23, 35 -- X-NAI-Spam-Rules: 1 Rules triggered 23, 35 -- RV3225=0 23, 35 -- Content-Transfer-Encoding: 8bit 23, 35 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n26EZMgj022519 ==============================End of - Headers==============================
Hello, I was wondering if anyone worked on imaging the drop size/micelles of liquid fabric softener. I just got the quanotmix imaging capsule system and was going to try it. Anyone use these systems with or without a stain on fabric softeners? Any advice greatly appreciated. Thanks. Gordon Vrdoljak.
==============================Original Headers============================== 1, 31 -- From doc.vrdoljak-at-gmail.com Fri Mar 6 12:05:13 2009 1, 31 -- Received: from yw-out-1718.google.com (yw-out-1718.google.com [74.125.46.156]) 1, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26I5D4R012009 1, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 12:05:13 -0600 1, 31 -- Received: by yw-out-1718.google.com with SMTP id 4so408581ywq.0 1, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 06 Mar 2009 10:05:12 -0800 (PST) 1, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 31 -- d=gmail.com; s=gamma; 1, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject 1, 31 -- :from:to:content-type:content-transfer-encoding; 1, 31 -- bh=kk5wwTF1DmneGllhNm85gHMyiB88AQXxOw2h18Ba4VE=; 1, 31 -- b=m39AJMPQ+6eAISFZ58AB7zCwlC6EMd9mgbHg4TBNz0Swq3wivcunazrU+pZJ6i23pr 1, 31 -- GVGG675WH3sikrI/ovaO5Yy7zz0X1gjvTQ5EYlmLWM85zjG9vWYgm7O/9CpmUMuVJDWL 1, 31 -- Y0pjVJZdOnr9jZYlcE5EiLjeQV5lbyBECxDKs= 1, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 31 -- d=gmail.com; s=gamma; 1, 31 -- h=mime-version:date:message-id:subject:from:to:content-type 1, 31 -- :content-transfer-encoding; 1, 31 -- b=enUDeU6VAdjIjAWUGIP+GHl1HYhpWt4ujtDh0Y6u0uuhFXBF3H0gQH4KCIz12uunO9 1, 31 -- 6qNI6r3prfye0aiD/t/Fk52GIoZnYeO9DptfviQ3l6mJP+4KtLRK4cKXlfSL/nDp7/sx 1, 31 -- bqJD8MSIwutdKMgezCKauiWzdQa/9hp+46O1U= 1, 31 -- MIME-Version: 1.0 1, 31 -- Received: by 10.142.241.10 with SMTP id o10mr1194094wfh.275.1236362712125; 1, 31 -- Fri, 06 Mar 2009 10:05:12 -0800 (PST) 1, 31 -- Date: Fri, 6 Mar 2009 10:05:12 -0800 1, 31 -- Message-ID: {fb272960903061005w300f037au7c74dd18a33df59-at-mail.gmail.com} 1, 31 -- Subject: Question about micelles in fabric softener 1, 31 -- From: Gordon Vrdoljak {doc.vrdoljak-at-gmail.com} 1, 31 -- To: Microscopy-at-microscopy.com 1, 31 -- Content-Type: text/plain; charset=ISO-8859-1 1, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From excite.compieterhenk-at-jmail.co.za Fri Mar 6 12:22:58 2009 Return-Path: {excite.compieterhenk-at-jmail.co.za} Received: from google.com ([88.227.95.152]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26IMs7a026242 for {microscopylistserverarchive-at-microscopy.com} ; Fri, 6 Mar 2009 12:22:57 -0600 Received: from [176.184.69.190] (HELO google.com) by deep-mud.org; Fri, 6 Mar 2009 20:23:02 +0200
Hi,
There are some additional possibilities:
LR White can be UV polymerized; it does not even require the benzoyl peroxide to be mixed in (and that gives it YEARS of shelf life at 4C....). A dual 4W "BLB" fluorescent unit a few inches above the samples is good; anything equivalent will work. You don't want it to polymerize too quickly so experiment. You can use aluminum weigh pans with a cover of Saran, Aclar, or Cellophane (good luck finding real cellophane today) film, or gelatine capsules - you need to exclude oxygen. Since you can embed from EtOH the harsher acetone or propylene oxide can be avoided.
Standard epoxy resins will also UV polymerize, similar conditions as above.
Even with heated polymerization, it it accelerated by higher temps but even 50C for longer times will work.
Depending on the resolution required, the replication of the surfaces with dental impression materials has given excellent results from plant surfaces. ====================================================================== A procedure for SEM of complex shoot structures applied to the inflorescence of snapdragon (Antirrhinum) P.B.Green and P.Linstead Protoplasma,1990, 158:33-38.
Kerr products http://www.kerrcasting.com/ has a list of world wide dealers on their web site.
Kerr Division of Sybron Corp 28200 Wick Rd, PO Box 455 Romulus, Mich. 48174 313-9467800
Also: Dentsply Caulk Dentsply International, Inc. Milford, DE 19963-0359 1-800-LD-CAULK
This is for Reprosil, a vinyl polysiloxane impression material. It comes in various viscosities.
Other sources: Exacta Dental Products (http://www.exactadp.com) 3M (http://www.3m.com/market/healthcare/dental2/prod_imprintII.html) ================================================
Dale
==============================Original Headers============================== 15, 20 -- From dac-at-research.umass.edu Fri Mar 6 13:56:00 2009 15, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Ju0QK029026 15, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 13:56:00 -0600 15, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 15, 20 -- (authenticated bits=0) 15, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n26JtxMJ023013 15, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 15, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 14:56:00 -0500 15, 20 -- Message-ID: {49B1805C.8000100-at-research.umass.edu} 15, 20 -- Date: Fri, 06 Mar 2009 14:58:20 -0500 15, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 15, 20 -- Reply-To: dac-at-research.umass.edu 15, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 15, 20 -- MIME-Version: 1.0 15, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 15, 20 -- Subject: Cuticle 15, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 20 -- Content-Transfer-Encoding: 7bit 15, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Sorry. The link for Kerr casting seems wrong - seems they moved into other areas. May be true of the other mfg info as well. I suggest a web search for "Dental Impression material"...
Dale
==============================Original Headers============================== 3, 20 -- From dac-at-research.umass.edu Fri Mar 6 14:05:07 2009 3, 20 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26K55Zj010381 3, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 14:05:06 -0600 3, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 3, 20 -- (authenticated bits=0) 3, 20 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n26K53jP003328 3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 3, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 15:05:04 -0500 3, 20 -- Message-ID: {49B1827C.7070004-at-research.umass.edu} 3, 20 -- Date: Fri, 06 Mar 2009 15:07:24 -0500 3, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 3, 20 -- Reply-To: dac-at-research.umass.edu 3, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.19) Gecko/20081204 SeaMonkey/1.1.14 3, 20 -- MIME-Version: 1.0 3, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 20 -- Subject: Cuticle (bad links....) 3, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
} LR White can be UV polymerized; it does not even require the benzoyl } peroxide to be mixed in (and that gives it YEARS of shelf life at } 4C....). A dual 4W "BLB" fluorescent unit a few inches above the samples } is good; anything equivalent will work. You don't want it to polymerize } too quickly so experiment. You can use aluminum weigh pans with a cover } of Saran, Aclar, or Cellophane (good luck finding real cellophane today) } film, or gelatine capsules - you need to exclude oxygen. Since you can } embed from EtOH the harsher acetone or propylene oxide can be avoided. } } Standard epoxy resins will also UV polymerize, similar conditions as above.
I agree that UV polymerization can be used quite effectively with the acrylics and Vestopal, a polyester resin.
I've not had much luck using UV to polymerize epoxy, however. Since UV does not penetrate very deeply into osmicated specimens (50-100 micrometer), specimens have to be really thin and you need to irradiate from as many sides as possible (or rotate the specimen). Even then.......
If you have a detailed protocol specifically for UV polymerization of epoxy resins, I would like to try it out.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I would freeze-dry or air-dry the leaves and look at them in SEM. If you can access to an environmental SEM you may look at fresh specimens also. Wax can cause separation of leaf tissue from adjacent resin. I believe that wax would not take up water soluble stain like UA and LC to be visible under TEM even if it is not dissolved during specimen preparation.
Ann Fook
Ann-Fook Yang, EM Unit | Unite EM Food Safety and Quality | Salubrité et qualité des aliments Agriculture and Agri-Food Canada | Agriculture et Agroalimentaire Canada Edifice K.W. Neatby Building, 960 Carling Av | 960 Boulevard Carling, Ottawa,Ontario K1A 0C6
ann-fook.yang-at-agr.gc.ca Telephone | Téléphone: 613-759-1638 Facsimile | Télécopieur: 613-759-1701 Teletypewriter | Téléimprimeur 613-759-7470 Government of Canada | Gouvernement du Canada
-----Original Message----- X-from: MARIE.CANTINO-at-uconn.edu [mailto:MARIE.CANTINO-at-uconn.edu] Sent: Friday, March 06, 2009 9:24 AM To: Yang, Ann-Fook
I am working with a student who is investigating plant cuticles, a coating on the outermost layer of cells that consists of the polymer cutin and waxes. When examining this feature with TEM the waxes are often lost. From what she has read, this occurs mainly during the resin polymerization stage. The waxes typically melt at 52-56° C. Currently she is using Spurrs resin, which has been recommended in the literature for the type of plants (mosses) that she is examining.
Possible approaches we are considering are:
1. Low temperature embedding using UV polymerization. We have equipment (Leica AFS) and experience with low temp embedding in Lowicryl HM-20 for immuno, but not for preserving waxes.
2. Microwave embedding in any resin. We have a Pella microwave with Coldspot, but haven't done much with plant tissue so far.
3. CryoSEM. We have a good FESEM, but do NOT have a cold stage, but would like to know whether anyone in this area (CT, MA, RI, NY) who does and would be able to help her with a one-time examination of her samples.
If anyone has used these or other techniques for preserving waxy cuticles or a similar material, we would be very interested in hearing about your experience.
Dr. Marie E. Cantino Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242 Phone: 860-486-3588 Fax: 860-486-6369
==============================Original Headers============================== 10, 17 -- From MARIE.CANTINO-at-uconn.edu Fri Mar 6 08:05:17 2009 10, 17 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26E5GPg007821 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 08:05:17 -0600 10, 17 -- Received: from d46h165.public.uconn.edu (d46h165.public.uconn.edu [137.99.46.165]) 10, 17 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n26E5F7l020707 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 09:05:16 -0500 10, 17 -- Message-Id: {FAF6C351-B2CD-4052-853A-5822964648A6-at-uconn.edu} 10, 17 -- From: Marie Cantino {MARIE.CANTINO-at-uconn.edu} 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 10, 17 -- Mime-Version: 1.0 (Apple Message framework v929.2) 10, 17 -- Subject: EM preservation of waxy plant cuticles 10, 17 -- Date: Fri, 6 Mar 2009 09:05:15 -0500 10, 17 -- X-Mailer: Apple Mail (2.929.2) 10, 17 -- Content-Transfer-Encoding: 8bit 10, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26E5GPg007821 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 22 -- From Ann-Fook.Yang-at-AGR.GC.CA Fri Mar 6 15:05:13 2009 24, 22 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118]) 24, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26L5CfT006926 24, 22 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 15:05:12 -0600 24, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 22 -- Content-class: urn:content-classes:message 24, 22 -- MIME-Version: 1.0 24, 22 -- Content-Type: text/plain; 24, 22 -- charset="iso-8859-1" 24, 22 -- Subject: RE: [Microscopy] EM preservation of waxy plant cuticles 24, 22 -- Date: Fri, 6 Mar 2009 16:05:07 -0500 24, 22 -- Message-ID: {773CE84BBA86484A9FEDE7741B77DDA4016173B4-at-onottaxms1.AGR.GC.CA} 24, 22 -- In-Reply-To: {200903061424.n26EOR0i022003-at-ns.microscopy.com} 24, 22 -- X-MS-Has-Attach: 24, 22 -- X-MS-TNEF-Correlator: 24, 22 -- Thread-Topic: [Microscopy] EM preservation of waxy plant cuticles 24, 22 -- Thread-Index: AcmeZ0RRxcQVYtoVQCyu6l4BEt2J9gAFuZtA 24, 22 -- From: "Yang, Ann-Fook" {Ann-Fook.Yang-at-AGR.GC.CA} 24, 22 -- To: {microscopy-at-microscopy.com} 24, 22 -- X-OriginalArrivalTime: 06 Mar 2009 21:05:07.0890 (UTC) FILETIME=[3AB81120:01C99E9F] 24, 22 -- Content-Transfer-Encoding: 8bit 24, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n26L5CfT006926 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both clei-at-illinois.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: clei-at-illinois.edu Name: changhui lei
Organization: U of Illinois at Urbana-Champaign
Title-Subject: [Filtered] Optimal lnes current for CM12/STEM
Question: Dear Colleagues,
We have an old Philiphs CM12 /STEM which has the twin objetctive lens. Recently the alignment is bad, and probably we lost the optimal objective lens current set by manufacture. The optimal objective lens current is usually obtained by press "Autofocus" key.
Here I am wondering if anybody who has a simlar microscope could tell me the optimal objective lens number.
You cna find the number by the following way: 1) Press "Autofocus" 2) On the right column of screen, press "Parameters" and you will go to parameters pages 3) on the left column of "parameters", there is "Display Currents". After pressing the "Display Currents", there are alots of lens current numbers show up.
} Hi John, } } We've only used UV polymerization in conjunction with epoxy for very } thin samples - single cells, or thin sections being "re-embedded" } after resin removal and immunolabeling or histochemistry - so Os } density was not a problem. } } Dale } } } bozzola-at-siu.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
The "AutoFocus" lens current on the CM series microscopes is set in the column alignment procedure (on the left side of the "Alignment" page). The procedure leads you through all the steps needed for all the lens presets and default deflector centering.
Basically, the idea is to take your sample, set a recognizable feature to the center of the screen and tilt. If the object moves away from the center, bring it back with the Z adjustment. This is known as setting the eucentric height. It sets the sample onto the rotation axis of the stage which is a reproduceable position. You then manually focus the image and note the focus current .
It is important to keep the sample at the eucentric position for magnification calibration. If you focus on a sample away from the eucentric position, the focal length of the objective lens is different causing a variation in the magnification. You can easily wind up with a 10% magnification error.
Cheers, Henk
clei-at-illinois.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both clei-at-illinois.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: clei-at-illinois.edu } Name: changhui lei } } Organization: U of Illinois at Urbana-Champaign } } Title-Subject: [Filtered] Optimal lnes current for CM12/STEM } } Question: Dear Colleagues, } } We have an old Philiphs CM12 /STEM which has the twin objetctive } lens. Recently the alignment is bad, and probably we lost the optimal } objective lens current set by manufacture. The optimal objective lens } current is usually obtained by press "Autofocus" key. } } Here I am wondering if anybody who has a simlar microscope could tell } me the optimal objective lens number. } } You cna find the number by the following way: } 1) Press "Autofocus" } 2) On the right column of screen, press "Parameters" and you will go } to parameters pages } 3) on the left column of "parameters", there is "Display Currents". } After pressing the "Display Currents", there are alots of lens } current numbers show up. } } I need the object and twin lens numbers. } } Thanks! } } Changhui } } } Login Host: 130.126.103.228 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 13, 11 -- From zaluzec-at-microscopy.com Fri Mar 6 17:45:08 2009 } 13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n26Nj66p025116 } 13, 11 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 17:45:07 -0600 } 13, 11 -- Mime-Version: 1.0 } 13, 11 -- Message-Id: {p06240803c5d765e05431-at-[206.69.208.22]} } 13, 11 -- Date: Fri, 6 Mar 2009 17:45:06 -0600 } 13, 11 -- To: microscopy-at-microscopy.com } 13, 11 -- From: clei-at-illinois.edu (by way of MicroscopyListserver) } 13, 11 -- Subject: viaWWW: Optimal lnes current for CM12/STEM } 13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at one. Lately it doesn't seem to be working."
==============================Original Headers============================== 10, 26 -- From colijn.1-at-osu.edu Fri Mar 6 19:08:32 2009 10, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2718VMj022615 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Mar 2009 19:08:32 -0600 10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 10, 26 -- id {01N69ZX43S8G8XH7T6-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 10, 26 -- Fri, 06 Mar 2009 20:08:31 -0500 (EST) 10, 26 -- Received: from [192.168.1.101] 10, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 10, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 10, 26 -- with ESMTPA id {01N69ZX3LRAO8X16KG-at-ecr6.ohio-state.edu} for 10, 26 -- microscopy-at-microscopy.com; Fri, 06 Mar 2009 20:08:30 -0500 (EST) 10, 26 -- Date: Fri, 06 Mar 2009 20:08:30 -0500 10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 26 -- Subject: Re: [Microscopy] viaWWW: Optimal lnes current for CM12/STEM 10, 26 -- In-reply-to: {200903062347.n26NlZIR027359-at-ns.microscopy.com} 10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 26 -- To: microscopy-at-microscopy.com 10, 26 -- Message-id: {49B1C90E.9060507-at-osu.edu} 10, 26 -- MIME-version: 1.0 10, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 10, 26 -- Content-transfer-encoding: 7bit 10, 26 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 26 -- References: {200903062347.n26NlZIR027359-at-ns.microscopy.com} ==============================End of - Headers==============================
Electroplated Nickel is ferromagnetic but, as far as I know, has no remanent magnetic field at room temperature. When examined in a high resolution SEM at 50-100 kX, is there any concern with this type of sample?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 3, 24 -- From donc-at-asmicro.com Sat Mar 7 09:12:26 2009 3, 24 -- Received: from smtp103.sbc.mail.re2.yahoo.com (smtp103.sbc.mail.re2.yahoo.com [68.142.229.102]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n27FCPrD008197 3, 24 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2009 09:12:26 -0600 3, 24 -- Received: (qmail 54931 invoked from network); 7 Mar 2009 15:12:25 -0000 3, 24 -- Received: from unknown (HELO asm15) (donc-at-68.51.122.238 with login) 3, 24 -- by smtp103.sbc.mail.re2.yahoo.com with SMTP; 7 Mar 2009 15:12:24 -0000 3, 24 -- X-YMail-OSG: H9Wis40VM1nuqD2nM0YWlPGoGBCmDejbdqbRr.5H4OIqjgxtx3G_xlgYbzOuhJYB9QS8tYdjAvUe7FOZ5JLohMxy612n0qGHA_T.Ml4pji.QHsPU_dmYx3ii_ci3U3jZN1rEQ2kFV4MtvP7WMVSeEotHEuig4bhuM97bQ3iNjlpb.phPE3ao1ukGekoi9_BA4Pyim4zQgBRtrEMocbWTLDzbUUvdchuMXCKXe9Ib9I3EPg-- 3, 24 -- X-Yahoo-Newman-Property: ymail-3 3, 24 -- Message-ID: {C0485DDD33CB4E44ACB17EEA32D78856-at-asm15} 3, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 3, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 3, 24 -- Subject: Nickel & SEM 3, 24 -- Date: Sat, 7 Mar 2009 10:12:03 -0500 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- format=flowed; 3, 24 -- charset="iso-8859-1"; 3, 24 -- reply-type=original 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Put a magnet towards the sample. If north or south attracts the sample, it is magnetic. If so, using a magnetic immersion lens SEM is likely going to be an issue. Depending on the mag and magnetic characteristics of the sample and WD, either nothing will happen or the column could be warped...bent. Not a good scenario.
For FEI/Philips SFEGs, use EDS mode. Poor resolution, poor S/N but the final lens magnetic field is off. For electroplated metals, it seems to me that a couple of KX ought to be enough. If not, use a LEO/Zeiss FESEM, in which case the sample characteristics are irrelevant.
gary g.
At 07:14 AM 3/7/2009, you wrote:
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==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Sat Mar 7 11:36:58 2009 9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n27HawRI009499 9, 20 -- for {microscopy-at-microscopy.com} ; Sat, 7 Mar 2009 11:36:58 -0600 9, 20 -- Message-Id: {200903071736.n27HawRI009499-at-ns.microscopy.com} 9, 20 -- Received: (qmail 23150 invoked from network); 7 Mar 2009 09:36:12 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 23145, pid: 23148, t: 0.1046s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by smtp2 with SMTP; 7 Mar 2009 09:36:12 -0800 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Sat, 07 Mar 2009 09:36:47 -0800 9, 20 -- To: donc-at-asmicro.com 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] Nickel & SEM 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200903071514.n27FEt30011166-at-ns.microscopy.com} 9, 20 -- References: {200903071514.n27FEt30011166-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
From russoantonio1979-at-libero.it Sun Mar 8 05:49:14 2009 Return-Path: {russoantonio1979-at-libero.it} Received: from google.com (203-59-176-214.dyn.iinet.net.au [203.59.176.214]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n28AnCB3004946 for {microscopylistserverarchive-at-microscopy.com} ; Sun, 8 Mar 2009 05:49:13 -0500 Received: from [152.67.59.182] (HELO google.com) by moldy-darkmeat.co.uk; Sun, 8 Mar 2009 19:49:12 +0900
Look at Single Crystal by Crystal Maker {http://www.crystalmaker.com/ singlecrystal/index.html} works for both Windows and Mac. There is a demo version.
Gordon
On Mar 4, 2009, at 8:27 PM, anita.garg-at-grc.nasa.gov wrote:
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==============================Original Headers============================== 6, 25 -- From gnord-at-mindspring.com Sun Mar 8 13:05:31 2009 6, 25 -- Received: from elasmtp-curtail.atl.sa.earthlink.net (elasmtp-curtail.atl.sa.earthlink.net [209.86.89.64]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n28I5VOl024956 6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 8 Mar 2009 13:05:31 -0500 6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 25 -- s=dk20050327; d=mindspring.com; 6, 25 -- b=idVA3CyKxwDx0IMIcUVSNa7oacjAgjbX0fZpFAyi9EvB1im9AWf0PnvM88WxOdi5; 6, 25 -- h=Received:Mime-Version:In-Reply-To:References:Content-Type:Message-Id:Content-Transfer-Encoding:From:Subject:Date:To:X-Mailer:X-ELNK-Trace:X-Originating-IP; 6, 25 -- Received: from [72.73.29.117] (helo=[192.168.254.10]) 6, 25 -- by elasmtp-curtail.atl.sa.earthlink.net with esmtpa (Exim 4.67) 6, 25 -- (envelope-from {gnord-at-mindspring.com} ) 6, 25 -- id 1LgNNV-0000Sr-2k; Sun, 08 Mar 2009 14:05:33 -0400 6, 25 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 25 -- In-Reply-To: {200903050127.n251RFPJ008426-at-ns.microscopy.com} 6, 25 -- References: {200903050127.n251RFPJ008426-at-ns.microscopy.com} 6, 25 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 25 -- Message-Id: {9FAD7904-C72B-45A0-ABE5-910B477933F1-at-mindspring.com} 6, 25 -- Content-Transfer-Encoding: 7bit 6, 25 -- From: Gordon Nord {gnord-at-mindspring.com} 6, 25 -- Subject: Re: [Microscopy] viaWWW: TEM: Electron diffraction Simulation program for PC 6, 25 -- Date: Sun, 8 Mar 2009 14:05:31 -0400 6, 25 -- To: anita.garg-at-grc.nasa.gov, Microscopy {microscopy-at-microscopy.com} 6, 25 -- X-Mailer: Apple Mail (2.753.1) 6, 25 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d8886924630f8852f17313f74797233710bf5b9b91c8690ac559350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 6, 25 -- X-Originating-IP: 72.73.29.117 ==============================End of - Headers==============================
It may be not the best, but the programme that I use, CaRIne Crystallography, is very useful. You can see a trial version at http://pagespro-orange.fr/carine.crystallography/
Best, Elena
---------------------------------------------------------------------------- ------- Prof. Elena BELLUSO - Ph.D. Mineralogy and Crystallography Dipartimento di Scienze Mineralogiche e Petrologiche Università degli Studi di Torino Via Valperga Caluso, 35 I-10125 TORINO - ITALIA tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 e-mail: elena.belluso-at-unito.it http://www.dsmp.unito.it ---------------------------------------------------------------------------- -------
-----Messaggio originale----- Da: gnord-at-mindspring.com [mailto:gnord-at-mindspring.com] Inviato: domenica 8 marzo 2009 19.06 A: elena.belluso-at-unito.it Oggetto: [Microscopy] Re: viaWWW: TEM: Electron diffraction Simulation program for PC
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Look at Single Crystal by Crystal Maker {http://www.crystalmaker.com/ singlecrystal/index.html} works for both Windows and Mac. There is a demo version.
Gordon
On Mar 4, 2009, at 8:27 PM, anita.garg-at-grc.nasa.gov wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both anita.garg-at-grc.nasa.gov as well as the } MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: anita.garg-at-grc.nasa.gov } Name: Anita Garg } } Organization: NASA GRC } } Title-Subject: [Filtered] TEM: Electron diffraction Simulation } program for PC } } Question: Dear Colleagues } What is the best electron-diffraction simulation program available } for PC these days? Earlier, the "Diffract" program used to have a Mac } version only; is there a PC version available now? } TIA, } Anita } } Login Host: 128.156.10.80 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Wed Mar 4 19:26:48 2009 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n251Qk9B006802 } 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 4 Mar 2009 19:26:48 } -0600 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240804c5d4dac1b5b9-at-[206.69.208.22]} } 6, 11 -- Date: Wed, 4 Mar 2009 19:26:46 -0600 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: anita.garg-at-grc.nasa.gov (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: TEM: Electron diffraction Simulation } program for PC } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
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==============================Original Headers============================== 19, 30 -- From elena.belluso-at-unito.it Mon Mar 9 02:42:33 2009 19, 30 -- Received: from mail-out.unito.it (opterone.unito.it [130.192.119.88]) 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n297gWUM007818 19, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 02:42:33 -0500 19, 30 -- Received: (from root-at-localhost) 19, 30 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) id n297gVpg015599 19, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 08:42:31 +0100 19, 30 -- Received: from BELLUSO (bellusop4.dsmp.unito.it [130.192.111.68]) 19, 30 -- by mail-out.unito.it (8.13.8/8.13.4/Debian-3sarge1) with ESMTP id n297gS54015553 19, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 08:42:28 +0100 19, 30 -- X-Icontrol: Sent by Inrete Icontrol 19, 30 -- From: "Elena Belluso" {elena.belluso-at-unito.it} 19, 30 -- To: {Microscopy-at-microscopy.com} 19, 30 -- References: {200903081806.n28I6EU5025482-at-ns.microscopy.com} 19, 30 -- Subject: Re: viaWWW: TEM: Electron diffraction Simulation program for PC 19, 30 -- Date: Mon, 9 Mar 2009 08:42:53 +0100 19, 30 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAL5JZe2RS1VMrhZqgyhCeBDCgAAAEAAAABAVmOpMHptOtHOf6M2rQCABAAAAAA==-at-unito.it} 19, 30 -- MIME-Version: 1.0 19, 30 -- Content-Type: text/plain; 19, 30 -- charset="iso-8859-1" 19, 30 -- X-Mailer: Microsoft Office Outlook 11 19, 30 -- In-Reply-To: {200903081806.n28I6EU5025482-at-ns.microscopy.com} 19, 30 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 19, 30 -- thread-index: AcmgGJz5RYso2LuXR8edKckwFSik9QAcTvMg 19, 30 -- Disposition-Notification-To: "Elena Belluso" {elena.belluso-at-unito.it} 19, 30 -- X-Inrete-Amavisjob-Virus-Scanned: PDAmail Multiple Antivirus with ClamAv 19, 30 -- X-Inrete-Amavisjob-Service-Runned: 6 (n297gS54015553) 19, 30 -- X-Inrete-Amavisjob-Service-Disabled: No Service disabled (n297gS54015553) 19, 30 -- Content-Transfer-Encoding: 8bit 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n297gWUM007818 ==============================End of - Headers==============================
I was wondering whether anyone has a high frame rate (20 to 30 fps at full resolution) monochrome camera they can recommend for fluorescence microscopy, with a resolution of at least 2 megapixels?
It seems there are a few around 1 megapixel, but it is hard to find anything higher resolution.
Regards,
Ben
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 {http://mrcanu.pharm.ox.ac.uk/}
==============================Original Headers============================== 8, 26 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 07:10:56 2009 8, 26 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29CAuE5004150 8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 07:10:56 -0500 8, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 8, 26 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69) 8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 8, 26 -- id 1LgeK1-0004Lo-TN 8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000 8, 26 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) 8, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 8, 26 -- (Exim 4.69) 8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 8, 26 -- id 1LgeK1-0004xU-6Z 8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000 8, 26 -- Message-ID: {49B50758.9070309-at-pharm.ox.ac.uk} 8, 26 -- Date: Mon, 09 Mar 2009 12:11:04 +0000 8, 26 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 8, 26 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 8, 26 -- MIME-Version: 1.0 8, 26 -- To: microscopy-at-microscopy.com 8, 26 -- Subject: Fast monochrome digital camera 8, 26 -- X-Enigmail-Version: 0.95.7 8, 26 -- Content-Type: text/plain; charset=ISO-8859-1 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- X-Oxford-Username: phar0293 ==============================End of - Headers==============================
Could you tell us more about your application? That would certainly help out a lot for the choice of cameras. What objective do you plan to use? Does your specimen emit a lot of photons?
You aren't likely to find a camera that can read out that many pixels at the rate you are interested in, at least not that I'm aware of.
Hopefully you either may not truly need that many pixels or that speed. Lots of biological questions don't, but there are certainly situations where one would like to push the envelope on both of those parameters as your are trying to do.
You can certainly reach that rate ~30 fps with many EMCCD cameras using the 512x512 16 um pixel array, as long as you have enough photons around. This is a likely true candidate for those rates with most biological questions. If you want to increase your pixels, then you can get 4x as many pixels with the EMCCD's at 1024x1024, but you'll likely sacrifice on speed by about 3x to ~10 fps.
Cheers,
-- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office (901) 495-2536 Cell (901) 603-3162 samuel.connell-at-stjude.org
On 3/9/09 6:20 AM, "ben.micklem-at-pharm.ox.ac.uk" {ben.micklem-at-pharm.ox.ac.uk} wrote:
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I was wondering whether anyone has a high frame rate (20 to 30 fps at full resolution) monochrome camera they can recommend for fluorescence microscopy, with a resolution of at least 2 megapixels?
It seems there are a few around 1 megapixel, but it is hard to find anything higher resolution.
Regards,
Ben
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 {http://mrcanu.pharm.ox.ac.uk/}
==============================Original Headers============================== 8, 26 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 07:10:56 2009 8, 26 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29CAuE5004150 8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 07:10:56 -0500 8, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 8, 26 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69) 8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 8, 26 -- id 1LgeK1-0004Lo-TN 8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000 8, 26 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) 8, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 8, 26 -- (Exim 4.69) 8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 8, 26 -- id 1LgeK1-0004xU-6Z 8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000 8, 26 -- Message-ID: {49B50758.9070309-at-pharm.ox.ac.uk} 8, 26 -- Date: Mon, 09 Mar 2009 12:11:04 +0000 8, 26 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 8, 26 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 8, 26 -- MIME-Version: 1.0 8, 26 -- To: microscopy-at-microscopy.com 8, 26 -- Subject: Fast monochrome digital camera 8, 26 -- X-Enigmail-Version: 0.95.7 8, 26 -- Content-Type: text/plain; charset=ISO-8859-1 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- X-Oxford-Username: phar0293 ==============================End of - Headers==============================
Thanks for your reply, Samuel, and those of other who replied directly.
The camera is to fulfill several roles, and it is leading to this hard compromise.
The camera has to provide a live view which is used for drawing on-screen very thin neuronal processes (axon and dendritic spines)- frame rate is crucial for fluid focusing and movement feedback. This is mainly currently bright-field, but may be fluorescence in the future. It also has to function as a fluorescence camera for digital stereological, again focusing feedback needs to have no delay at all. Ideally it would be colour for the first use, and monochrome for the second.
Previously, these problems were overcome using a CRT module that projects the computers display image down a drawing tube, so the finest of axons could be drawn at the full capabilities of the optics of the microscope. These CRT unitsare no longer produced, so we have to use bring the microscope's image into the computer, instead of the other way around.
I think we will have to compromise on the frame rate, because the solutions that are available, e.g. pco.1600 (1600x1200 at 30fps) is probably too expensive (£13,500).
Ben
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 {http://mrcanu.pharm.ox.ac.uk/}
Connell, Samuel wrote: } Hi Ben, } } Could you tell us more about your application? That would certainly help out a lot for the choice of cameras. What objective do you plan to use? Does your specimen emit a lot of photons? } } You aren't likely to find a camera that can read out that many pixels at the rate you are interested in, at least not that I'm aware of. } } Hopefully you either may not truly need that many pixels or that speed. Lots of biological questions don't, but there are certainly situations where one would like to push the envelope on both of those parameters as your are trying to do. } } You can certainly reach that rate ~30 fps with many EMCCD cameras using the 512x512 16 um pixel array, as long as you have enough photons around. This is a likely true candidate for those rates with most biological questions. If you want to increase your pixels, then you can get 4x as many pixels with the EMCCD's at 1024x1024, but you'll likely sacrifice on speed by about 3x to ~10 fps. } } Cheers, } } -- } Samuel A. Connell } Director of Light Microscopy } Cell & Tissue Imaging Center } St. Jude Children's Research Hospital } 262 Danny Thomas Place } Memphis, TN 38105-3678 } Office (901) 495-2536 } Cell (901) 603-3162 } samuel.connell-at-stjude.org } } } } } On 3/9/09 6:20 AM, "ben.micklem-at-pharm.ox.ac.uk" {ben.micklem-at-pharm.ox.ac.uk} wrote: } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was wondering whether anyone has a high frame rate (20 to 30 fps at } full resolution) monochrome camera they can recommend for fluorescence } microscopy, with a resolution of at least 2 megapixels? } } It seems there are a few around 1 megapixel, but it is hard to find } anything higher resolution. } } } Regards, } } } Ben } } } -- } Imaging Technician } MRC Anatomical Neuropharmacology Unit, Mansfield Road, } Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 } {http://mrcanu.pharm.ox.ac.uk/}
==============================Original Headers============================== 9, 28 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 13:32:08 2009 9, 28 -- Received: from relay1.mail.ox.ac.uk (relay1.mail.ox.ac.uk [129.67.1.165]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29IW8OX013324 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 13:32:08 -0500 9, 28 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 9, 28 -- by relay1.mail.ox.ac.uk with esmtp (Exim 4.69) 9, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 9, 28 -- id 1LgkGl-0002HY-6N 9, 28 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 18:32:07 +0000 9, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) 9, 28 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 9, 28 -- (Exim 4.69) 9, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 9, 28 -- id 1LgkGl-0000ZT-8t 9, 28 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 18:32:07 +0000 9, 28 -- Message-ID: {49B560A7.1060006-at-pharm.ox.ac.uk} 9, 28 -- Date: Mon, 09 Mar 2009 18:32:07 +0000 9, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 9, 28 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 9, 28 -- MIME-Version: 1.0 9, 28 -- To: microscopy-at-microscopy.com 9, 28 -- Subject: Re: [Microscopy] Fast monochrome digital camera 9, 28 -- References: {C5DA8EA0.5AEC%Samuel.Connell-at-stjude.org} 9, 28 -- In-Reply-To: {C5DA8EA0.5AEC%Samuel.Connell-at-stjude.org} 9, 28 -- X-Enigmail-Version: 0.95.7 9, 28 -- Content-Type: text/plain; charset=ISO-8859-1 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-Oxford-Username: phar0293 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Mechanical Polishing of Ultra Thin Wafers
Question: I am mechanically polishing an 8 layer packaged die. The Si-C discs are like mac trucks to the approximately 40 micron layered individual die! I have switched to diamond lapping films but I am still having a massive amount of chip outs and cracking. Is anyone willing to fill in a fellow polisher on the secret to obtaining a beautiful, mirror-like finish to an extremely difficult packaged die cross section?
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Email: kjl226-at-vt.edu Name: Kathy J. Lowe
Organization: Vet. School at Virginia Tech
Title-Subject: [Filtered] Freeze Fracture machine
Question: My name is Kathy Lowe. I'm an Electron Microscopist at Virginia Tech. I am trying to locate a Balzer's Freeze Fracture instrument for one of our graduate students. He is working with cell culture. He is growing RB4(rat brain endothelial cells) and astrocytes on culture plate inserts. The inserts are made of HA(mixed cellulose esters) and PCF(tissue culture-treated Isopore). He is interested in the blood-brain barrier and wants to visualize the connections between the two cell types using the freeze fracture method. I've worked on a Balzer's 400T in the past.
Does anyone know how or where I can find and use a Freeze Fracture instrument?
Mechanical polishing is frustrating, futile, and lots of other adverbs.
However, depending how you have set up your specimen for polishing, it can at times produce useable results. The trick is to use a cover glass between the on the top of the die. Then, the die is hot waxed to a 90 degree stub. Then, the sandwich is polished. Leave a bit of the sandwich sticking out so it can be polished. I've had iffy success with this. It depends on where the stack uses W plugs or not. Even with W, it just depends. The other factor is the nature of the barrier metal layer type. The silicides and salicides also come into play.
gary g.
At 04:53 PM 3/9/2009, you wrote:
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==============================Original Headers============================== 11, 21 -- From gary-at-gaugler.com Mon Mar 9 19:03:11 2009 11, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2A03B9m024026 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 19:03:11 -0500 11, 21 -- Message-Id: {200903100003.n2A03B9m024026-at-ns.microscopy.com} 11, 21 -- Received: (qmail 22834 invoked from network); 9 Mar 2009 17:02:11 -0700 11, 21 -- Received: by simscan 1.1.0 ppid: 22831, pid: 22832, t: 0.1053s 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 21 -- by smtp2 with SMTP; 9 Mar 2009 17:02:11 -0700 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Mon, 09 Mar 2009 17:03:04 -0700 11, 21 -- To: adenarollins-at-hotmail.com 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 21 -- Subject: Re: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin 11, 21 -- Wafers 11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 21 -- In-Reply-To: {200903092353.n29Nrx2c008878-at-ns.microscopy.com} 11, 21 -- References: {200903092353.n29Nrx2c008878-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Adena; I have seen stacked die packages that had the dies bonded with a soft epoxy that would likely not support the inner die during polishing. If that is causing the problem you are experiencing, ion polishing with for example a JEOL SM-09010 Cross Section Polisher could help. However, if there are voids between the die, you will need to fill them by vacuum infusing epoxy.
John Mardinly, Numonyx
-----Original Message----- X-from: adenarollins-at-hotmail.com [mailto:adenarollins-at-hotmail.com] Sent: Monday, March 09, 2009 5:05 PM To: MARDINLY, A
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Title-Subject: [Filtered] Mechanical Polishing of Ultra Thin Wafers
Question: I am mechanically polishing an 8 layer packaged die. The Si-C discs are like mac trucks to the approximately 40 micron layered individual die! I have switched to diamond lapping films but I am still having a massive amount of chip outs and cracking. Is anyone willing to fill in a fellow polisher on the secret to obtaining a beautiful, mirror-like finish to an extremely difficult packaged die cross section?
Codonics NP-1660 printer. About 6 years but has not been used for the past year. Includes 3 boxes of 1660B-A Direct Vista paper and 2 boxes of 1600P-A paper with color ribbon.
If interested, please respond directly to me. Please remove [microscopy] from the subject line.
John Catino
Specialty Minerals, Inc. MINTEQ International, Inc. Easton, PA
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==============================Original Headers============================== 9, 16 -- From John.Catino-at-Mineralstech.com Tue Mar 10 09:16:41 2009 9, 16 -- Received: from exprod8og105.obsmtp.com (exprod8og105.obsmtp.com [64.18.3.90]) 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2AEGbxv017808 9, 16 -- for {Microscopy-at-Microscopy.com} ; Tue, 10 Mar 2009 09:16:39 -0500 9, 16 -- Received: from source ([65.209.12.89]) by exprod8ob105.postini.com ([64.18.7.12]) with SMTP 9, 16 -- ID DSNKSbZ2QlWNlL7B8wIPMOF6hwPNr6Go491J-at-postini.com; Tue, 10 Mar 2009 07:16:40 PDT 9, 16 -- Subject: Codonics NP-1660 Printer Available 9, 16 -- To: Microscopy-at-Microscopy.com 9, 16 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 9, 16 -- Message-ID: {OF6BB0B5C9.6E93EE0C-ON85257575.004E31FD-85257575.004E69E5-at-mineralstech.com} 9, 16 -- From: John.Catino-at-Mineralstech.com 9, 16 -- Date: Tue, 10 Mar 2009 10:16:29 -0400 9, 16 -- X-MIMETrack: Serialize by Router on dpn1/Minerals_Tech(Release 6.5.4FP2|September 12, 2005) at 9, 16 -- 03/10/2009 10:16:40 AM 9, 16 -- MIME-Version: 1.0 9, 16 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
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Question: One of the temperature sensor/control units on our Emitech K1250 has gone belly up (the temperature sensor is kaput). We've pulled it apart and determined that we cannot fix the multilayer control board.
That being said, I'm looking for a spare temperature controller, specifically:
Company: Red Lion Model T48 1/16 DIN Temperature Controller
This is the unit that came standard with the unit and would fit back in nicely.
Anyone have a junked out Emitech kicking around they might want to part ways with, or one of these units you don't need?
Adena: how thick is your stack and what's the package type? And John Mardinly is correct; if you have a softer material in contact with a hard material, the hard material will crack and chip on the side in contact with the soft one if the direction of polish is going that way. Usually the polishing wheel is running CCW and your section is on the wheel with the active side of the die facing into the turning direction, i.e. your wheel spins to the right and the section faces to the left. The Si die is hard but the die attach epoxy is soft, so when the wheel/polishing material pushed past the die into the epoxy, the die had no support and will chip. This is not usually a big problem as the front side of the die is usually the one of interest. Since you have SiC spacers, that's harder than anything in the rest of the package and nearly as hard the diamond you're polishing with. You don't say if you are using diamond lapping film or diamond suspension. I usually always prefer diamond lapping film on a glass platen with plenty of lubricant (usually running DI water). You definitely want to keep any SiC chips from becoming embedded in the film or elsewhere in your package or mounting media. I rarely pot samples up in epoxy any more. If the package has enough structural integrity, I usually treat it like a bare die and section using old Technology Associates' brass fixtures that have been modified to be easier to use. If the package is not strong enough in itself, I usually sandwich it between a piece of glass microscope slide and a glass cover slip using EpoxyBond 110 (or M-Bond, if you prefer). Allied High Tech and Accelerated Analysis (among others) have fixtures of a similar type to the old Technology Associates' ones, which are no longer available. http://www.acceleratedanalysis.com/xs_basics.html is the URL for a nice overview of polishing basics. The references at the end are very informative. I've been polishing bare dice and packages for 25+ years and I'll be happy to give any help I can.
adenarollins-at-hotmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both adenarollins-at-hotmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: adenarollins-at-hotmail.com } Name: Adena Rollins } } Title-Subject: [Filtered] Mechanical Polishing of Ultra Thin Wafers } } Question: I am mechanically polishing an 8 layer packaged die. The } Si-C discs are like mac trucks to the approximately 40 micron layered } individual die! I have switched to diamond lapping films but I am } still having a massive amount of chip outs and cracking. Is anyone } willing to fill in a fellow polisher on the secret to obtaining a } beautiful, mirror-like finish to an extremely difficult packaged die } cross section? } } Thank you in advance for any information! } } Login Host: 63.163.107.100 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Mon Mar 9 18:51:59 2009 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29Npv1t004063 } 6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 18:51:58 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240800c5db5c050970-at-[206.69.208.22]} } 6, 11 -- Date: Mon, 9 Mar 2009 18:51:56 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: adenarollins-at-hotmail.com (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Mechanical Polishing of Ultra Thin Wafers } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 5, 22 -- From r-holdford-at-ti.com Tue Mar 10 17:35:51 2009 5, 22 -- Received: from comal.ext.ti.com (comal.ext.ti.com [198.47.26.152]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2AMZnLK029474 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Mar 2009 17:35:50 -0500 5, 22 -- Received: from dlep33.itg.ti.com ([157.170.170.112]) 5, 22 -- by comal.ext.ti.com (8.13.7/8.13.7) with ESMTP id n2AMZgJn019311; 5, 22 -- Tue, 10 Mar 2009 17:35:47 -0500 5, 22 -- Received: from [156.117.248.174] (localhost [127.0.0.1]) 5, 22 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id n2AMZf31027964; 5, 22 -- Tue, 10 Mar 2009 17:35:42 -0500 (CDT) 5, 22 -- Message-ID: {49B6EB3D.4040002-at-ti.com} 5, 22 -- Date: Tue, 10 Mar 2009 17:35:41 -0500 5, 22 -- From: Becky Holdford {r-holdford-at-ti.com} 5, 22 -- Organization: SC Packaging Development -- FA Development 5, 22 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 5, 22 -- MIME-Version: 1.0 5, 22 -- To: adenarollins-at-hotmail.com, MSA Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] viaWWW: Mechanical Polishing of Ultra Thin Wafers 5, 22 -- References: {200903092352.n29NqAO6004301-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200903092352.n29NqAO6004301-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: yvan-at-yvanlindekens.be Name: Yvan
Title-Subject: [Filtered] User manual for Shandon HistoCenter 2 wanted...
Question: If you have one (or a xerox copy) to spare I would be very, very, very obliged!
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Wed Mar 11 08:04:37 2009 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BD4a9K000700 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 08:04:37 -0500 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p06240800c5dd6748dcdd-at-[206.69.208.22]} 8, 11 -- Date: Wed, 11 Mar 2009 08:04:35 -0500 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: yvan-at-yvanlindekens.be (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: Shandon HistoCenter 2 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
From ckjsimcwpnlvssdwod.x-at-skypipeline.com Wed Mar 11 11:20:28 2009 Return-Path: {ckjsimcwpnlvssdwod.x-at-skypipeline.com} Received: from google.com (dsl.dynamic81215244238.ttnet.net.tr [81.215.244.238] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BGKQXR021799 for {microscopylistserverarchive-at-microscopy.com} ; Wed, 11 Mar 2009 11:20:27 -0500 Received: from [95.174.92.100] (HELO google.com) by carefulsneeze.org; Wed, 11 Mar 2009 18:20:25 +0200
We are putting our new TIRF system through its paces (Nikon TI motorized) and need some help from experienced users: 1. We can in theory use any of the laser lines from our laser launch (405, 488, 563, 640) for doing TIRF. In terms of switching between the lines, is it realistic to expect that the cubes and dichroic mirrors will be matched in position well enough that you could switch between cubes without having to realign the system? As things are now, if I switch cubes in the turret, the beam position changes significantly.
2. What is the best sample for testing TIRF? I have been using fluorescent beads in an 8 well chamber slide so far, but I am never 100% convinced as to when I am really in TIRF mode. WIth 3um beads, they settle rapidly and I have never seen a situation where the bottom of the bead is sharp and the top of the bead invisible as you would expect for TIRF. With 0.5um beads, they move so fast that they don't seem to become sharp near the coverslip unless stuck. I am guessing that somewhere around 1um might be ideal to see them appear and disappear in the near coverslip position. Presumably, when in TIRF, beads stuck to the coverslip will go into and out of focus as you move the objective, but nothing else will come into focus in other planes. Is that the best diagnostic?
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Wed Mar 11 16:28:59 2009 5, 19 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2BLSxWh031235 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 16:28:59 -0500 5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) 5, 19 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n2BLSvl3022467 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 11 Mar 2009 17:28:57 -0400 5, 19 -- Message-Id: {9CD840A9-322A-45DF-8C92-259BEE146C2E-at-uconn.edu} 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) 5, 19 -- Subject: TIRF setup help 5, 19 -- Date: Wed, 11 Mar 2009 17:28:57 -0400 5, 19 -- X-Mailer: Apple Mail (2.930.3) 5, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 5, 19 -- X-UConn-MailScanner: Found to be clean 5, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
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Organization: Centers for Disease Control and Prevention (CDC)
Title-Subject: [Filtered] Annual meeting of the Southeastern Microscopy Society
Question: The annual meeting of the Southeastern Microscopy Society will be held this year in Athens, GA, on May 27-29. There will be four excellent invited speakers, including Sara Miller (Duke University), Jay Jerome (Vanderbilt University), Wilma Lingle (Mayo Clinic) and Yiping Zhao (University of Georgia). There will also be student presentations and contributed talks. In addition, several workshops will be held on Wednesday, May 27.
Please visit out website at http://www.southeasternmicroscopy.org/index-2.html for further information.
I am looking into the possibility of having a Nitrogen gas supply near a widefield inverted microscope so that I can present hypoxic conditions around a well plate. The microscope is surrounded by a full Solent Scientific environmental chamber with the well plate surrounded by a secondary chamber than is around eight inches square.
Has anyone any experience of setting up such conditions and if so what are the steps involved to keep your health and safety manager happy?
Many thanks
Steve
Steve Bagley Imaging Facility Cancer Research UK Paterson Institute for Cancer Research University of Manchester Wilmslow Road Manchester M20 9BX UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
==============================Original Headers============================== 11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009 11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2C9S2q2012729 11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 04:28:03 -0500 11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] helo=PMAIL01.picr.man.ac.uk) 11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD)) 11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} ) 11, 28 -- id 1LhhCs-000EFd-5L 11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02 +0000 11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 11, 28 -- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="us-ascii" 11, 28 -- Subject: nitrogen supply at the microscope 11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000 11, 28 -- Message-ID: {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk} 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- Importance: normal 11, 28 -- Priority: normal 11, 28 -- Thread-Topic: nitrogen supply at the microscope 11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg 11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk} 11, 28 -- To: {Microscopy-at-microscopy.com} 11, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2C9S2q2012729 ==============================End of - Headers==============================
There should be some sort of risk assessment (in UK law). I assume (but I'm not familiar with the apparatus) that there could be a potential for nitrogen venting out of the apparatus or supply lines. If there is then one thing to look at is the potential for dropping the overall oxygen content by 1 or 2% in the room - you will need to check those figures. But, if it can happen, then an oxygen depletion alarm would be very important as a first step.
Hopefully this will keep you happy too, because people do die from oxygen depletion because there is no warning.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: SBagley-at-picr.man.ac.uk
Hi Steve, Here's my 2 cents worth: A number of years ago a company I worked for stored a large LN2 tank in the TEM dark room (I don't remember why). Concern was expressed that, if spilt while decanting, the LN2 would reduce the O2 content to non-survivable levels and we should install an O2 alarm.
Our safety officer did a quick calculation based on the size of the tank, size of the room and assumed all the LN2 would be instantly converted to gas. Based on his calculations O2 levels would have drop less than 1% and that was the end of that.
I'd start with that calculation, let your safety people calculate that out for you...makes them feel involved. Worse case, how O2 would be replaced If your system dumped the maximum amount of N2 into your work environment.
By the way, we did end up with a O2 sensor on an semi-enclosed loading dock, because that's were the fill fines for LN2 were located. We lost that argument.
Stay safe..... Frank
SBagley-at-picr.man. ac.uk To 03/12/2009 05:46 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] nitrogen supply at the SBagley-at-picr.man. microscope ac.uk
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi
I am looking into the possibility of having a Nitrogen gas supply near a widefield inverted microscope so that I can present hypoxic conditions around a well plate. The microscope is surrounded by a full Solent Scientific environmental chamber with the well plate surrounded by a secondary chamber than is around eight inches square.
Has anyone any experience of setting up such conditions and if so what are the steps involved to keep your health and safety manager happy?
Many thanks
Steve
Steve Bagley Imaging Facility Cancer Research UK Paterson Institute for Cancer Research University of Manchester Wilmslow Road Manchester M20 9BX UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
==============================Original Headers============================== 11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009 11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2C9S2q2012729 11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 04:28:03 -0500 11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] helo=PMAIL01.picr.man.ac.uk) 11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD)) 11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} ) 11, 28 -- id 1LhhCs-000EFd-5L 11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02 +0000 11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 11, 28 -- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="us-ascii" 11, 28 -- Subject: nitrogen supply at the microscope 11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000 11, 28 -- Message-ID: {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk} 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- Importance: normal 11, 28 -- Priority: normal 11, 28 -- Thread-Topic: nitrogen supply at the microscope 11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg 11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk} 11, 28 -- To: {Microscopy-at-microscopy.com} 11, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2C9S2q2012729 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009 32, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CAwULP002858 32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 05:58:30 -0500 32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com} 32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope 32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com 32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 32, 22 -- Message-ID: {OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lincolnelectric.com} 32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400 32, 22 -- From: Frank_Karl-at-lincolnelectric.com 32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM, 32, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM 32, 22 -- MIME-Version: 1.0 32, 22 -- Content-Type: text/plain; 32, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
From rogn-at-apol.com.tw Thu Mar 12 07:05:51 2009 Return-Path: {rogn-at-apol.com.tw} Received: from google.com (p42005-adsau12honb6-acca.tokyo.ocn.ne.jp [220.97.197.5]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CC5kDx026700 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 12 Mar 2009 07:05:47 -0500 Received: from [111.144.76.113] (HELO google.com) by wise-white.com; Thu, 12 Mar 2009 21:05:48 +0900 Message-ID: {00000001C92D08A448363700} Reply-To: Richmal Winton {eunice.nicklas1012-at-gmail.com}
Good morning, dear Steve (good afternoon), dear all,
I'm wondering wether you are talking about huge amounts of liquid nitrogen in a tank (volume to be filled say 15, 40, or 200 Liters??) for cryo-applications by means of special preparation apparatusses (eg. cryomicrotome, cryo-etching etc.) or even at the EM itself (cryostage, cooling of detectors etc.).
Yes, I remember that similar calculations were done for my Cryo-tank (Dewars 10 L and 40 L) and the EM-Lab by a {safety officer} (at that time in 1981 there wasn't really any "safety officer" in our institutions but the person who did the calculations, besides UO2-Ac-radiation items was the academic physicist working in the Radiology Department).
As I understood the initial question, Steve, you are dealing with the need of creating an anoxic/hypoxic environment in a chamber/housing (environmental chamber) enclosing the whole {LM Light Microscopical} widefield inverted microscope or at least the experimental setting with the 8 x 8 inches (= 20.32 cm, cf.: http://www.onlineconversion.com/length_common.htm )?
Correct?
If so, my 2 (Euro-)cents worth is / are:
A0) remembering that (pure) nitrogen (vapor from LN2) is heavier than "AIR" in our surroundings.
A1) nobody } normally { would think about placing a dewar or overhead storage tank above your working place.
A2) most smaller dewars (for long, mid and short term storage) are placed at the floor (say opening of the dewar device reaches height of knees or at the maximum breast height, if researcher is in a sitting position)... one exception seems to be the LN2 Cooling Dewar device for my old ZEISS EM109 (vintage 1979) which is positioned approx. 20 cm above my head when sitting in front of the micr's examination window):
The evaporation of the (approximately 1 L of) LN2 (contained in the dewar which has some sort of lid with a central pore and 90 degr. angled tube-outlet directed backwards) is low and usually mixes up very rapidly with the "normal room air", especially if the room does have at least a kind of "permanent ventilation").
Nevertheless I confess that some guests visiting the Lab and sitting WITH me at the EM (with the LN2 dewar mounted) sometimes lamented on a "sudden tiredness" they felt over the minutes sitting there) (;-((
A3) If you use liquid nitrogen from a storage tank/dewar you certainly will need and have to use (very) special equipment (flanges, fittings, hoses, reducing valves, gloves and PSA protecting from splashing, etc).
A4) even if you use (only) a e. g. 5 or 10 L LN2-storing dewar under normal conditions (i.e. storage at RT and proper/safe stand at the floor bottom) the evaporation rate into the room IMO is minimal.
BUT: You don't need to store liquid nitrogen in a tank in your working room if you experiment with an- or hypoxic conditions in a small(er) and controllable testing system (chamber).
B1) for your experimental set-up perhaps you go better with pressurized gas bottles or cylinders. Nitrogen gas is available in several kinds of quality (for your use I recommend at least quality } 5.0 { as we can get such here in Europe from our Lab gas-suppliers) and capacities (from hand-hold sizes to stand-cylinders).
B2) in either case (A-B) you certainly need (on the chamber) at least bore holes for filling and venting, controlling instruments/reduction (perhaps micro-)valves.
B3) a Venting hole/port with controlling valve should be directed (best with not too long tubing) down to the floor.
I do have implemented such a solution for venting the EM-column sections and the inbuilt 35 mm camera receptaculum for now 29 years without any complication (BTW: the death of any fly visiting my lab-rooms unfortunately seems attributable to other causes than "nitrogen overload"). Also I have used that/similar design for -/+ controlled excluding oxygen from embedding resins or overlaying bottles/Lab flasks with oxygen-sensible contents with "nearly inert" gas (if I do't need to use Freon or freon-like substitutes) .
You perhaps should discuss such a solution also with your safety officer....maybe "nasty" questions then are limited.
Best wishes and regards,
Wolfgang MUSS Salzburg-Austria
OR Dr. phil. Wolfgang Muss Head of EM-Lab Institute of Pathology, SALK (Gen. Hospital) (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively (same Lab, same address)
Paracelsus Medical Private University (PMU) Univ.-Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
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} -----Ursprüngliche Nachricht----- } Von: Frank_Karl-at-lincolnelectric.com } [mailto:Frank_Karl-at-lincolnelectric.com] } Gesendet: Donnerstag, 12. März 2009 12:02 } An: Muß Wolfgang } Betreff: [Microscopy] Re: Nitrogen supply at the microscope } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Hi Steve, } Here's my 2 cents worth: } A number of years ago a company I worked for stored a large } LN2 tank in the TEM dark room (I don't remember why). } Concern was expressed that, if spilt while decanting, the LN2 } would reduce the O2 content to non-survivable } levels and we should install an O2 alarm. } } Our safety officer did a quick calculation based on the size } of the tank, size of the room and assumed all the LN2 would } be instantly converted to gas. Based on his calculations O2 } levels would have drop less than 1% and that was the end of that. } } I'd start with that calculation, let your safety people } calculate that out for you...makes them feel involved. Worse } case, how O2 would be replaced If your system dumped the } maximum amount of N2 into your work environment. } } By the way, we did end up with a O2 sensor on an } semi-enclosed loading dock, because that's were the fill } fines for LN2 were located. We lost that argument. } } Stay safe..... } Frank } } } } } } SBagley-at-picr.man. } } ac.uk } } } To } 03/12/2009 05:46 } frank_karl-at-lincolnelectric.com } AM } cc } } } } Subject } Please respond to [Microscopy] nitrogen } supply at the } SBagley-at-picr.man. microscope } } ac.uk } } } } } } } } } } } } } } } } } } } -------------------------------------------------------------- } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } } } Hi } } I am looking into the possibility of having a Nitrogen gas } supply near a } widefield inverted microscope so that I can present hypoxic conditions } around a well plate. The microscope is surrounded by a full Solent } Scientific environmental chamber with the well plate surrounded by a } secondary chamber than is around eight inches square. } } Has anyone any experience of setting up such conditions and if so what } are the steps involved to keep your health and safety manager happy? } } Many thanks } } Steve } } } } } Steve Bagley } Imaging Facility } Cancer Research UK } Paterson Institute for Cancer Research } University of Manchester } Wilmslow Road } Manchester } M20 9BX } UK } -------------------------------------------------------- } This email is confidential and intended solely for the use of } the person(s) } ('the intended recipient') to whom it was addressed. Any } views or opinions } presented are solely those of the author and do not } necessarily represent } those of the Paterson Institute for Cancer Research or the } University of } Manchester. It may contain information that is privileged & } confidential } within the meaning of applicable law. Accordingly any dissemination, } distribution, copying, or other use of this message, or any of its } contents, by any person other than the intended recipient may } constitute a } breach of civil or criminal law and is strictly prohibited. } If you are NOT } the intended recipient please contact the sender and dispose } of this e-mail } as soon as possible. } } } ==============================Original } Headers============================== } 11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009 } 11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk } [130.88.200.94]) } 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n2C9S2q2012729 } 11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 } 04:28:03 -0500 } 11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] } helo=PMAIL01.picr.man.ac.uk) } 11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 } (FreeBSD)) } 11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} ) } 11, 28 -- id 1LhhCs-000EFd-5L } 11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar } 2009 09:28:02 } +0000 } 11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 } 11, 28 -- Content-class: urn:content-classes:message } 11, 28 -- MIME-Version: 1.0 } 11, 28 -- Content-Type: text/plain; } 11, 28 -- charset="us-ascii" } 11, 28 -- Subject: nitrogen supply at the microscope } 11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000 } 11, 28 -- Message-ID: } {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk} } 11, 28 -- X-MS-Has-Attach: } 11, 28 -- X-MS-TNEF-Correlator: } 11, 28 -- Importance: normal } 11, 28 -- Priority: normal } 11, 28 -- Thread-Topic: nitrogen supply at the microscope } 11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg } 11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk} } 11, 28 -- To: {Microscopy-at-microscopy.com} } 11, 28 -- X-UoM: Scanned by the University Mail System. See } http://www.itservices.manchester.ac.uk/email/filtering/informa } tion/ for } details. } 11, 28 -- Content-Transfer-Encoding: 8bit } 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n2C9S2q2012729 } ==============================End of - } Headers============================== } } } -- } ************************************************************* } Note: } The information contained in this message may be } privileged and confidential and protected from disclosure. If } the reader of this message is not the intended recipient, or } an employee or agent responsible for delivering this message } to the intended recipient, you are hereby notified that any } dissemination, distribution or copying of this communication } is strictly prohibited. If you have received this } communication in error, please notify us immediately by } replying to the message and deleting it from your computer. } Thank you, } The Lincoln Electric Company } ************************************************************** } } } ==============================Original } Headers============================== } 32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009 } 32, 22 -- Received: from lincolnelectric.com } (smtp1.lincolnelectric.com [64.109.211.114]) } 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n2CAwULP002858 } 32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar } 2009 05:58:30 -0500 } 32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com} } 32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope } 32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com } 32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 } 32, 22 -- Message-ID: } {OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lin } colnelectric.com} } 32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400 } 32, 22 -- From: Frank_Karl-at-lincolnelectric.com } 32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln } Electric/US(Release 8.0.1|February } 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, } 32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM, } 32, 22 -- Itemize by Router on Notescom1/Lincoln } Electric/US(Release 8.0.1|February } 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, } 32, 22 -- Serialize by Router on Notescom1/Lincoln } Electric/US(Release 8.0.1|February } 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, } 32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM } 32, 22 -- MIME-Version: 1.0 } 32, 22 -- Content-Type: text/plain; } 32, 22 -- charset="US-ASCII" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 34, 36 -- From W.Muss-at-salk.at Thu Mar 12 07:50:40 2009 34, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 34, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CCoc28028093 34, 36 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 07:50:39 -0500 34, 36 -- Received: from localhost (localhost [127.0.0.1]) 34, 36 -- by hermes.salk.at (Postfix) with ESMTP id 361AAC388C 34, 36 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:50:37 +0100 (CET) 34, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 34, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 34, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 34, 36 -- with ESMTP id Q9KVwiahvcsB for {microscopy-at-microscopy.com} ; 34, 36 -- Thu, 12 Mar 2009 13:50:36 +0100 (CET) 34, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 34, 36 -- by hermes.salk.at (Postfix) with ESMTP id C0A72C3886 34, 36 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:50:36 +0100 (CET) 34, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 34, 36 -- Thu, 12 Mar 2009 13:50:37 +0100 34, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 34, 36 -- Content-class: urn:content-classes:message 34, 36 -- MIME-Version: 1.0 34, 36 -- Content-Type: text/plain; 34, 36 -- charset="iso-8859-1" 34, 36 -- Subject: [Microscopy] Re: Nitrogen supply at the microscope 34, 36 -- Date: Thu, 12 Mar 2009 13:50:36 +0100 34, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D08D3-at-N1RZ116.lksdom21.lks.local} 34, 36 -- In-Reply-To: {200903121101.n2CB1j1i011158-at-ns.microscopy.com} 34, 36 -- X-MS-Has-Attach: 34, 36 -- X-MS-TNEF-Correlator: 34, 36 -- Thread-Topic: [Microscopy] Re: Nitrogen supply at the microscope 34, 36 -- Thread-Index: AcmjAs5LgCkLoy6rR5WAC6QUxLC8XAAAMQgA 34, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 34, 36 -- To: {microscopy-at-microscopy.com} 34, 36 -- X-OriginalArrivalTime: 12 Mar 2009 12:50:37.0019 (UTC) FILETIME=[23FAF2B0:01C9A311] 34, 36 -- X-Scanned-By: SALK-Content-Filter 34, 36 -- Content-Transfer-Encoding: 8bit 34, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2CCoc28028093 ==============================End of - Headers==============================
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Email: florian.dallatorre-at-mat.ethz.ch Name: Florian Dalla Torre
Organization: Dept. Materials, ETH Zurich, Switzerland
Title-Subject: [Filtered] Tenupol Electropolisher
Question: Hi,
Does anyone have a spare cooling coil of a Tenupol jetpolisher of Struers? Ours broke
Frank; Intel actually had a fatality in Malaysia about 15 years ago due to improper set up of a nitrogen fill station in an enclosed room, so I think that your argument that you lost about the O2 sensor, the safety folks did the right thing for you.
John Mardinly, Numonyx
-----Original Message----- X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com] Sent: Thursday, March 12, 2009 4:05 AM To: MARDINLY, A
Hi Steve, Here's my 2 cents worth: A number of years ago a company I worked for stored a large LN2 tank in the TEM dark room (I don't remember why). Concern was expressed that, if spilt while decanting, the LN2 would reduce the O2 content to non-survivable levels and we should install an O2 alarm.
Our safety officer did a quick calculation based on the size of the tank, size of the room and assumed all the LN2 would be instantly converted to gas. Based on his calculations O2 levels would have drop less than 1% and that was the end of that.
I'd start with that calculation, let your safety people calculate that out for you...makes them feel involved. Worse case, how O2 would be replaced If your system dumped the maximum amount of N2 into your work environment.
By the way, we did end up with a O2 sensor on an semi-enclosed loading dock, because that's were the fill fines for LN2 were located. We lost that argument.
Stay safe..... Frank
SBagley-at-picr.man.
ac.uk
To 03/12/2009 05:46 frank_karl-at-lincolnelectric.com
AM cc
Subject Please respond to [Microscopy] nitrogen supply at the SBagley-at-picr.man. microscope
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi
I am looking into the possibility of having a Nitrogen gas supply near a widefield inverted microscope so that I can present hypoxic conditions around a well plate. The microscope is surrounded by a full Solent Scientific environmental chamber with the well plate surrounded by a secondary chamber than is around eight inches square.
Has anyone any experience of setting up such conditions and if so what are the steps involved to keep your health and safety manager happy?
Many thanks
Steve
Steve Bagley Imaging Facility Cancer Research UK Paterson Institute for Cancer Research University of Manchester Wilmslow Road Manchester M20 9BX UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
==============================Original Headers============================== 11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009 11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2C9S2q2012729 11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 04:28:03 -0500 11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] helo=PMAIL01.picr.man.ac.uk) 11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD)) 11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} ) 11, 28 -- id 1LhhCs-000EFd-5L 11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02 +0000 11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 11, 28 -- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="us-ascii" 11, 28 -- Subject: nitrogen supply at the microscope 11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000 11, 28 -- Message-ID: {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk} 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- Importance: normal 11, 28 -- Priority: normal 11, 28 -- Thread-Topic: nitrogen supply at the microscope 11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg 11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk} 11, 28 -- To: {Microscopy-at-microscopy.com} 11, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2C9S2q2012729 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009 32, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CAwULP002858 32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 05:58:30 -0500 32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com} 32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope 32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com 32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 32, 22 -- Message-ID: {OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lincolnelectr ic.com} 32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400 32, 22 -- From: Frank_Karl-at-lincolnelectric.com 32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM, 32, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM 32, 22 -- MIME-Version: 1.0 32, 22 -- Content-Type: text/plain; 32, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 52, 29 -- From A.MARDINLY-at-numonyx.com Thu Mar 12 12:59:11 2009 52, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 52, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CHxBNq013058 52, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 12 Mar 2009 12:59:11 -0500 52, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 52, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 41DA4144040; 52, 29 -- Thu, 12 Mar 2009 12:01:57 -0400 (EDT) 52, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 52, 29 -- Thu, 12 Mar 2009 13:59:10 -0400 52, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 52, 29 -- Content-class: urn:content-classes:message 52, 29 -- MIME-Version: 1.0 52, 29 -- Content-Type: text/plain; 52, 29 -- charset="us-ascii" 52, 29 -- Subject: RE: [Microscopy] Re: nitrogen supply at the microscope 52, 29 -- Date: Thu, 12 Mar 2009 13:58:12 -0400 52, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39B68-at-EXDRESBENMX012.numonyx.local} 52, 29 -- In-Reply-To: {200903121105.n2CB5I1l021078-at-ns.microscopy.com} 52, 29 -- X-MS-Has-Attach: 52, 29 -- X-MS-TNEF-Correlator: 52, 29 -- Thread-Topic: [Microscopy] Re: nitrogen supply at the microscope 52, 29 -- Thread-Index: AcmjAnE7r4KYjsQ3SI+gyUlfeH2hwAAOU6LA 52, 29 -- References: {200903121105.n2CB5I1l021078-at-ns.microscopy.com} 52, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 52, 29 -- To: {Frank_Karl-at-lincolnelectric.com} 52, 29 -- Cc: {Microscopy-at-Microscopy.com} 52, 29 -- X-OriginalArrivalTime: 12 Mar 2009 17:59:10.0744 (UTC) FILETIME=[3F053180:01C9A33C] 52, 29 -- Content-Transfer-Encoding: 8bit 52, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2CHxBNq013058 ==============================End of - Headers==============================
As far as I remember, one liter of liquid nitrogen produces about 700 liters of gaseous nitrogen, which then doesn't contain any oxygen. In addition, the nitrogen vapor is cold, and will sink to the floor. If you experience a sudden drop in oxygen concentration, you may loose conscience, even if the relative oxygen concentration was still within the breathable ranges. If you then fall to the ground, you are in the nitrogen vapor, where you will not get any oxygen. If you stay there for 2 minutes without immediate help, you are gone. The minimum required O2 level for survival are somewhere around 10% (?). Normal values are 22%.
I heard the rumors of an accident, where a graduate student in Germany tried to refill a LN2 dewar in a cold-room (low ventilation) from another dewar one Saturday. He overfilled, the LN2 spilled onto the floor, he lost conscience. Another graduate student saw that, tried to reanimate his fellow in the coldroom, and both were found dead on Monday morning.
Portable O2 meters can be found here: http://www.ceainstr.com/pdf_datasheets/gasman2_Info.pdf These are portable devices with a digital display of the oxygen concentration. They are about the size of a calculator, powered by three AA batteries. They cost $614 per device, with about 12 months of life time of the oxygen sensor, and $110 replacement costs for the Oxygen sensor alone.
I have no affiliation with that company what so ever, except that we have a few of these gas meters.
Henning.
Henning Stahlberg, Molecular & Cellular Biology, Briggs Hall 5, University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab), Fax: +1-530-752 3085 mailto:HStahlberg-at-ucdavis.edu, Skype:henningstahlberg http://stahlberglab.org http://2dx.org
==============================Original Headers============================== 12, 21 -- From HStahlberg-at-ucdavis.edu Thu Mar 12 13:37:25 2009 12, 21 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CIbNtf028144 12, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:37:24 -0500 12, 21 -- Received: from [169.237.214.89] ([169.237.214.89]) 12, 21 -- (authenticated bits=0) 12, 21 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n2CIbLPT009447 12, 21 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 12, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 11:37:21 -0700 (PDT) 12, 21 -- Message-Id: {B6B52287-A6BF-45F5-ADA7-1388684B707A-at-ucdavis.edu} 12, 21 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu} 12, 21 -- To: Microscopy-at-microscopy.com 12, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 12, 21 -- Content-Transfer-Encoding: 7bit 12, 21 -- Mime-Version: 1.0 (Apple Message framework v930.4) 12, 21 -- Subject: Re: [Microscopy] nitrogen supply at the microscope 12, 21 -- Date: Thu, 12 Mar 2009 11:37:21 -0700 12, 21 -- X-Mailer: Apple Mail (2.930.4) 12, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on av5 12, 21 -- X-Virus-Status: Clean 12, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32 ==============================End of - Headers==============================
On Mar 12, 2009, at 11:37 AM, HStahlberg-at-ucdavis.edu wrote:
} As far as I remember, one liter of liquid nitrogen produces about } 700 liters of gaseous nitrogen
} The minimum required O2 level for survival are somewhere around 10% } (?).
Dear Henning, I think you are correct about the N2 gas/liquid ratio and too optimistic about minimum O2. I remember a figure of 16% for survival and 10% to keep a candle flame lit. On the other hand, people can climb to ~6.5 km without additional O2, so if one equates the partial pressure of O2 at that height with a percentage at sea level that gives the same partial pressure, one can calculate an upper limit. (The calculation will be left as an exercise for the reader. :-)) Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Mar 12 13:55:24 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CItNVk011150 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 13:55:24 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 3314F2E50B4D 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 11:55:21 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 45CD6328075 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 11:55:15 -0700 (PDT) 6, 22 -- Message-Id: {65F7E6C4-F7BA-4652-919F-39EC1F51CD17-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200903121837.n2CIbaiX028303-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] Re: nitrogen supply at the microscope 6, 22 -- Date: Thu, 12 Mar 2009 11:55:15 -0700 6, 22 -- References: {200903121837.n2CIbaiX028303-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Would anyone care to comment on fixatives that do not contain formaldehyde or alcohol? A commercial product of this type is known as Histochoice MB. Pros/cons? We are interested in non-dehydrating fixation/embedding, i.e., aqueous resins. -- Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30, Room 310 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax 301-402-0396
==============================Original Headers============================== 1, 17 -- From rjpalmer-at-dir.nidcr.nih.gov Thu Mar 12 15:00:29 2009 1, 17 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 1, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CK0REd027299 1, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:00:29 -0500 1, 17 -- X-IronPortListener: NIH_Relay 1, 17 -- X-SBRS: None 1, 17 -- X-IronPort-AV: E=Sophos;i="4.38,352,1233550800"; 1, 17 -- d="scan'208";a="72724884" 1, 17 -- Received: from nidcr106-134.nidcr.nih.gov (HELO [128.231.106.134]) ([128.231.106.134]) 1, 17 -- by nihrelayxway2.hub.nih.gov with ESMTP; 12 Mar 2009 16:00:16 -0400 1, 17 -- Mime-Version: 1.0 1, 17 -- Message-Id: {p06210200c5df19851909-at-[128.231.106.134]} 1, 17 -- Date: Thu, 12 Mar 2009 16:00:15 -0400 1, 17 -- To: Microscopy-at-microscopy.com 1, 17 -- From: "Robert J. Palmer Jr." {rjpalmer-at-dir.nidcr.nih.gov} 1, 17 -- Subject: Histochoice fixative 1, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Well, this is a fine kettle of fish I got myself into.
Please pass me the salt, so I can eat my words. Several people were kind enough to e-mail me about my error and I thank them. Their correction will enable me to be safer. It bothers me that I trusted the GY safety people and that I had come to depend on their evaluation. I can only wonder what other safety related problems were missed.
thanks again
Frank
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==============================Original Headers============================== 6, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 15:19:10 2009 6, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CKJ9RN009192 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:19:09 -0500 6, 22 -- In-Reply-To: {200903121845.n2CIjSW2008710-at-ns.microscopy.com} 6, 22 -- Subject: nitrogen supply at the microscope 6, 22 -- To: Microscopy-at-microscopy.com 6, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 6, 22 -- Message-ID: {OF3B6AEDEA.9DD3EEC6-ON85257577.006DDBAF-85257577.006F971D-at-lincolnelectric.com} 6, 22 -- Date: Thu, 12 Mar 2009 16:18:53 -0400 6, 22 -- From: Frank_Karl-at-lincolnelectric.com 6, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 22 -- 07, 2008) at 03/12/2009 04:18:52 PM, 6, 22 -- CD-MIME complete at 03/12/2009 04:18:52 PM, 6, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 22 -- 07, 2008) at 03/12/2009 04:18:52 PM, 6, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 6, 22 -- 07, 2008) at 03/12/2009 04:18:52 PM, 6, 22 -- Serialize complete at 03/12/2009 04:18:52 PM 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
You should also not discount the possibility of a pressure relief valve failure or poor insulation causing higher than normal boil off.
I once had a tank's high pressure safety go off. Sounded like a jet plane and it filled the room with nitrogen gas in a matter of minutes. Called the fire department and they advised us to evacuate the room and go outside. The came screaming up with full breathing apparat. and checked the room for us before we went back in. No harm done, but pretty exciting.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 8, 44 -- From jkrupp-at-deltacollege.edu Thu Mar 12 16:55:18 2009 8, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2CLt5iP026014 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 16:55:18 -0500 8, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 8, 44 -- by localhost (Email Security Appliance) with SMTP id CB0691B9464_9B97F17B 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:03 +0000 (GMT) 8, 44 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 8, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 8C8E3183B0B_9B97F17F 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:01 +0000 (GMT) 8, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 8, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 8, 44 -- with ESMTP id 46042684 for microscopy-at-microscopy.com; Thu, 12 Mar 2009 14:54:11 -0700 8, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 8, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 8, 44 -- ESMTP id KGEXEU00.2M0 for {microscopy-at-microscopy.com} ; Thu, 12 8, 44 -- Mar 2009 14:37:42 -0700 8, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 0F9BA8F7501D 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:11 -0700 (PDT) 8, 44 -- X-Virus-Scanned: amavisd-new at 8, 44 -- X-Spam-Flag: NO 8, 44 -- X-Spam-Score: -2.499 8, 44 -- X-Spam-Level: 8, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 8, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1] 8, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 8, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 8, 44 -- with ESMTP id nPmPguQidFlT for {microscopy-at-microscopy.com} ; 8, 44 -- Thu, 12 Mar 2009 14:54:10 -0700 (PDT) 8, 44 -- Received: from [172.20.3.146] (unknown [172.20.3.146]) 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 2AE0A8F7501E 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:10 -0700 (PDT) 8, 44 -- Message-Id: {21D383E5-4DE8-4383-BA34-3F1210F08BA5-at-deltacollege.edu} 8, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 8, 44 -- To: microscopy-at-microscopy.com 8, 44 -- In-Reply-To: {200903122026.n2CKQYmD021645-at-ns.microscopy.com} 8, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 44 -- Content-Transfer-Encoding: 7bit 8, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3) 8, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope 8, 44 -- Date: Thu, 12 Mar 2009 14:54:09 -0700 8, 44 -- References: {200903122026.n2CKQYmD021645-at-ns.microscopy.com} 8, 44 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Sometimes good intentions go awry as well. Some years ago when a freezer or dewar failed a lab person stored items in a portable dewar and then placed it in a cold room. It cold in there and should last longer--right? Later someone else entered the room and nearly passed out due to the lack of oxygen. He was smart and had experience at high altitudes so managed to get out and survive. Whew! Larry
jkrupp-at-deltacollege.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } You should also not discount the possibility of a pressure relief } valve failure or poor insulation causing higher than normal boil off. } } I once had a tank's high pressure safety go off. Sounded like a jet } plane and it filled the room with nitrogen gas in a matter of minutes. } Called the fire department and they advised us to evacuate the room } and go outside. The came screaming up with full breathing apparat. and } checked the room for us before we went back in. No harm done, but } pretty exciting. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original Headers============================== } 8, 44 -- From jkrupp-at-deltacollege.edu Thu Mar 12 16:55:18 2009 } 8, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2CLt5iP026014 } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 16:55:18 -0500 } 8, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 8, 44 -- by localhost (Email Security Appliance) with SMTP id CB0691B9464_9B97F17B } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:03 +0000 (GMT) } 8, 44 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) } 8, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 8C8E3183B0B_9B97F17F } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:01 +0000 (GMT) } 8, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) } 8, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) } 8, 44 -- with ESMTP id 46042684 for microscopy-at-microscopy.com; Thu, 12 Mar 2009 14:54:11 -0700 } 8, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by } 8, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 8, 44 -- ESMTP id KGEXEU00.2M0 for {microscopy-at-microscopy.com} ; Thu, 12 } 8, 44 -- Mar 2009 14:37:42 -0700 } 8, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 0F9BA8F7501D } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:11 -0700 (PDT) } 8, 44 -- X-Virus-Scanned: amavisd-new at } 8, 44 -- X-Spam-Flag: NO } 8, 44 -- X-Spam-Score: -2.499 } 8, 44 -- X-Spam-Level: } 8, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, } 8, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1] } 8, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 8, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 8, 44 -- with ESMTP id nPmPguQidFlT for {microscopy-at-microscopy.com} ; } 8, 44 -- Thu, 12 Mar 2009 14:54:10 -0700 (PDT) } 8, 44 -- Received: from [172.20.3.146] (unknown [172.20.3.146]) } 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 2AE0A8F7501E } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:10 -0700 (PDT) } 8, 44 -- Message-Id: {21D383E5-4DE8-4383-BA34-3F1210F08BA5-at-deltacollege.edu} } 8, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 8, 44 -- To: microscopy-at-microscopy.com } 8, 44 -- In-Reply-To: {200903122026.n2CKQYmD021645-at-ns.microscopy.com} } 8, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 8, 44 -- Content-Transfer-Encoding: 7bit } 8, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 8, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope } 8, 44 -- Date: Thu, 12 Mar 2009 14:54:09 -0700 } 8, 44 -- References: {200903122026.n2CKQYmD021645-at-ns.microscopy.com} } 8, 44 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers============================== }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 6, 41 -- From Larry.Ackerman-at-ucsf.edu Thu Mar 12 17:19:22 2009 6, 41 -- Received: from emfmcb01.ucsfmedicalcenter.org (EMFMCB01.ucsfmedicalcenter.org [64.54.46.97]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CMJLWJ008140 6, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 17:19:22 -0500 6, 41 -- Received: from [64.54.35.209] by emfmcb01.ucsfmedicalcenter.org with 6, 41 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2)); 6, 41 -- Thu, 12 Mar 2009 15:19:17 -0700 6, 41 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B 6, 41 -- X-AuditID: 403623d1-a7451bb00000708a-90-49b98a655eb0 6, 41 -- Received: from EXHT02.net.ucsf.edu (unknown [64.54.247.219]) by 6, 41 -- vsobmcb01.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id 6, 41 -- 27FF715C7 for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:19:17 6, 41 -- -0700 (PDT) 6, 41 -- Received: from EXVS05.net.ucsf.edu (64.54.128.150) by 6, 41 -- EXHT02.net.ucsf.edu (64.54.247.219) with Microsoft SMTP Server id 6, 41 -- 8.1.340.0; Thu, 12 Mar 2009 15:19:16 -0700 6, 41 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by 6, 41 -- EXVS05.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 12 Mar 6, 41 -- 2009 15:18:29 -0700 6, 41 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 6, 41 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 12 Mar 6, 41 -- 2009 15:18:28 -0700 6, 41 -- Message-ID: {49B98A34.2010006-at-ucsf.edu} 6, 41 -- Date: Thu, 12 Mar 2009 15:18:28 -0700 6, 41 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 6, 41 -- Reply-to: larry.ackerman-at-ucsf.edu 6, 41 -- Organization: UCSF, NeuroAnatomy 6, 41 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 6, 41 -- MIME-Version: 1.0 6, 41 -- To: Microscopy-at-microscopy.com 6, 41 -- Subject: Re: [Microscopy] Re: nitrogen supply at the microscope 6, 41 -- References: {200903122203.n2CM3spi003857-at-ns.microscopy.com} 6, 41 -- In-Reply-To: {200903122203.n2CM3spi003857-at-ns.microscopy.com} 6, 41 -- X-OriginalArrivalTime: 12 Mar 2009 22:18:28.0719 (UTC) 6, 41 -- FILETIME=[784BC7F0:01C9A360] 6, 41 -- X-Brightmail-Tracker: AAAAAQ3stXE= 6, 41 -- X-WSS-ID: 65A755EF1O868741-01-01 6, 41 -- Content-Type: text/plain; 6, 41 -- charset=iso-8859-1; 6, 41 -- format=flowed 6, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are thinking about the setting up the same hypoxic thing here. We have just ordered a Zeiss [PeCon] system that includes variable CO2 and a plate cover that goes over the culture vessels to keep the 5% CO2 passing over the top of the wells only [i.e. not filling the entire incubator enclosure]. Sounds like you have the Solent equivalent that relies on a 5% CO2 cylinder and coils of tubing [our Zeiss system uses 100% CO2 at pressure via a half inch/reduction pipe and a Zeiss mechanical/electrical controller to set the air out to whatever %CO2 you want].
Although we a way off from implementing 'hypoxia' here, no doubt we would use a small standard regulated 100% nitrogen [no oxygen] BOC gas cylinder to create our hypoxic air by mixing it somehow with the %CO2/air [not sure how yet]. Back in the 1980s we set 5% CO2 levels by gas bubblers and flow meters and I imagine we would have a similar simple mixer this time.
Given that our microscope rooms [and incubator rooms] run happily with rather more toxic 100% CO2 supplies and with minimal concerns from our health and safety [as they are all correctly installed and inspected regularly], I don't envisage any problems at all installing a bijou N2 gas cylinder [chained to the wall] - the volumes drawn off are very small [a few cm3 per minute once purged] at the culture vessel media interface and the air exchange rate of the air conditioning in the microscope room is very high anyway. We wouldn't use any 'liquid nitrogen' tanks as such, so the risk assessment would be: there isn't any really, other than those associated with the cylinder trolley, wall clamp and a very heavy high pressure gas cylinder. We don't have any CO2 monitors or % O2 monitors in our cell culture incubator rooms or microscope room at present [and don't intend to], although naturally the latter are in the rooms where our large liquid nitrogen tissue storage tanks are.
Besides I suppose if a 1/2 inch pressurised 100% CO2 pipe ruptured in the cell culture incubator rooms you'd get out rather than wait for any gas monitor to warn you [the gas escape makes a very loud noise]. A large volume of liquid nitrogen can go to gas far quicker than block of dry ice [if you drop them], and so can be a greater hazard in a lift etc.., but neither risks are relevant to N2 or CO2 gas cylinder hazards. I guess our hypoxic air over the cultures would be 1% to 15% O2 [haven't thought about %CO2].
For CO2 cylinders BOC state: Ensure adequate ventilation. Carbon dioxide monitoring is recommended if used or stored in a confined space. Carbon dioxide Occupational Exposure Standard (OES): Long Term Exposure Limit (LTEL) 5000vpm Short Term Exposure Limit (STEL) 15000vpm
For N2 [no oxygen] BOC just say Personal protection: Ensure adequate ventilation.
A typical small N2 cylinder used would be similar to our CO2 cylinders: VB/LB 94x14cm net=22kg CO2=6.4kg.
N2, unlike CO2 is odourless though. Naturally I will discuss this all with our safety officer and make a detailed N2 safety assessment though, but I can't see it being a particular problem given the low N2 gas volumes used [as the CO2 case is already accepted]. Perhaps I've got my cm3 N2 gas volumes per min way out [and it's 99% N2/CO2 not 78% N2/CO2], but I don't think so.
It would be intersting to hear how you get on.
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
Sent: 12 March 2009 11:05 To: kjmorris-at-well.ox.ac.uk
Hi Steve, Here's my 2 cents worth: A number of years ago a company I worked for stored a large LN2 tank in the TEM dark room (I don't remember why). Concern was expressed that, if spilt while decanting, the LN2 would reduce the O2 content to non-survivable levels and we should install an O2 alarm.
Our safety officer did a quick calculation based on the size of the tank, size of the room and assumed all the LN2 would be instantly converted to gas. Based on his calculations O2 levels would have drop less than 1% and that was the end of that.
I'd start with that calculation, let your safety people calculate that out for you...makes them feel involved. Worse case, how O2 would be replaced If your system dumped the maximum amount of N2 into your work environment.
By the way, we did end up with a O2 sensor on an semi-enclosed loading dock, because that's were the fill fines for LN2 were located. We lost that argument.
Stay safe..... Frank
SBagley-at-picr.man. ac.uk To 03/12/2009 05:46 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] nitrogen supply at the SBagley-at-picr.man. microscope ac.uk
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi
I am looking into the possibility of having a Nitrogen gas supply near a widefield inverted microscope so that I can present hypoxic conditions around a well plate. The microscope is surrounded by a full Solent Scientific environmental chamber with the well plate surrounded by a secondary chamber than is around eight inches square.
Has anyone any experience of setting up such conditions and if so what are the steps involved to keep your health and safety manager happy?
Many thanks
Steve
Steve Bagley Imaging Facility Cancer Research UK Paterson Institute for Cancer Research University of Manchester Wilmslow Road Manchester M20 9BX UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
==============================Original Headers============================== 11, 28 -- From SBagley-at-picr.man.ac.uk Thu Mar 12 04:28:04 2009 11, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2C9S2q2012729 11, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 04:28:03 -0500 11, 28 -- Received: from jill.picr.man.ac.uk ([130.88.233.248] helo=PMAIL01.picr.man.ac.uk) 11, 28 -- by probity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD)) 11, 28 -- (envelope-from {SBagley-at-picr.man.ac.uk} ) 11, 28 -- id 1LhhCs-000EFd-5L 11, 28 -- for Microscopy-at-microscopy.com; Thu, 12 Mar 2009 09:28:02 +0000 11, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 11, 28 -- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="us-ascii" 11, 28 -- Subject: nitrogen supply at the microscope 11, 28 -- Date: Thu, 12 Mar 2009 09:30:14 -0000 11, 28 -- Message-ID: {096D992D69D1CE4E863A3758C7BDB641019F0482-at-PMAIL01.picr.man.ac.uk} 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- Importance: normal 11, 28 -- Priority: normal 11, 28 -- Thread-Topic: nitrogen supply at the microscope 11, 28 -- Thread-Index: Acmi9Hs2yYXMXVl8QKKcwBZxkFcQhAAAEgGg 11, 28 -- From: "Steve Bagley" {SBagley-at-picr.man.ac.uk} 11, 28 -- To: {Microscopy-at-microscopy.com} 11, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2C9S2q2012729 ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 22 -- From frank_karl-at-lincolnelectric.com Thu Mar 12 05:58:30 2009 32, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CAwULP002858 32, 22 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 05:58:30 -0500 32, 22 -- In-Reply-To: {200903120946.n2C9kjSh025703-at-ns.microscopy.com} 32, 22 -- Subject: Re: [Microscopy] nitrogen supply at the microscope 32, 22 -- To: SBagley-at-picr.man.ac.uk, Microscopy-at-microscopy.com 32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 32, 22 -- Message-ID: {OFCA85E0C6.9F0747DD-ON85257577.003ABF9F-85257577.003C4006-at-lincolnelectric.c om} 32, 22 -- Date: Thu, 12 Mar 2009 06:58:06 -0400 32, 22 -- From: Frank_Karl-at-lincolnelectric.com 32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- CD-MIME complete at 03/12/2009 06:58:07 AM, 32, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 03/12/2009 06:58:07 AM, 32, 22 -- Serialize complete at 03/12/2009 06:58:07 AM 32, 22 -- MIME-Version: 1.0 32, 22 -- Content-Type: text/plain; 32, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 63, 24 -- From kjmorris-at-well.ox.ac.uk Thu Mar 12 19:13:36 2009 63, 24 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 63, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2D0Damn024867 63, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 19:13:36 -0500 63, 24 -- Received: from localhost ([127.0.0.1] helo=morse.well.ox.ac.uk) 63, 24 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 63, 24 -- id 1Lhv1r-0004s1-Gn 63, 24 -- for Microscopy-at-microscopy.com; Fri, 13 Mar 2009 00:13:35 +0000 63, 24 -- Received: from 91.125.104.49 63, 24 -- (SquirrelMail authenticated user kjmorris) 63, 24 -- by morse.well.ox.ac.uk with HTTP; 63, 24 -- Fri, 13 Mar 2009 00:13:35 -0000 (GMT) 63, 24 -- Message-ID: {1808.91.125.104.49.1236903215.squirrel-at-morse.well.ox.ac.uk} 63, 24 -- Date: Fri, 13 Mar 2009 00:13:35 -0000 (GMT) 63, 24 -- Subject: RE: [Microscopy] Re: nitrogen supply at the microscope 63, 24 -- From: "Keith J Morris" {kjmorris-at-well.ox.ac.uk} 63, 24 -- To: Microscopy-at-microscopy.com 63, 24 -- Reply-To: kjmorris-at-well.ox.ac.uk 63, 24 -- User-Agent: SquirrelMail/1.4.13 63, 24 -- MIME-Version: 1.0 63, 24 -- Content-Type: text/plain;charset=iso-8859-15 63, 24 -- Content-Transfer-Encoding: 8bit 63, 24 -- X-Priority: 3 (Normal) 63, 24 -- Importance: Normal ==============================End of - Headers==============================
At one of CSIRO's labs in Geelong (near Melbourne), a CSIRO staff member died because none of the 3 safety mechanisms in the basement holding the LN2 freezers was working properly. He stepped down into the basement, passed out immediately and that was that - this happened about 8-9 years ago. Since then, the organisation has been extremely careful about LN2, and we are no longer allowed to store large volumes inside. OK, it's a pain, but you can see where they're coming from. Only takes a couple of people to get annoyed with a beeping O2 sensor (this happens to ours when it's time to replace the sensor) and turn it off and you've got a recipe for disaster.
Logically, all the safety stuff we have in our lab is completely over the top, but the answer to that is - we can't allow another accident like this one.
Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 13/03/09 5:08 AM, "A.MARDINLY-at-numonyx.com" {A.MARDINLY-at-numonyx.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Frank; } Intel actually had a fatality in Malaysia about 15 years ago due } to improper set up of a nitrogen fill station in an enclosed room, so I } think that your argument that you lost about the O2 sensor, the safety } folks did the right thing for you. } } John Mardinly, } Numonyx } } -----Original Message----- } X-from: Frank_Karl-at-lincolnelectric.com } [mailto:Frank_Karl-at-lincolnelectric.com] } Sent: Thursday, March 12, 2009 4:05 AM } To: MARDINLY, A } Subject: [Microscopy] Re: nitrogen supply at the microscope } }
Does anyone has information on the grease(s) used to lubricate the specimen advance (=section thickness) mechanism of a Jung Tetrander microtome? A picture of that particular model can be found here: http://www.yvanlindekens.be/microtomen/microtomen.htm
This microtome has more or less the same specimen advance mechanism as the Reichert/Jung Hn-40 sledge microtome, so I suppose the same lubricants can be applied (??) but I don't have any information on those either...
Same question on lmuibricants for the Leitz 1300.
If anyone would happen to have a user or repair manual for the Tetrander and/or the Leitz 1300 I would really appreciate a copy of it :-). Of course I'll pay the costs.
Many thanks in advance!
Yvan.
==============================Original Headers============================== 12, 20 -- From yvan_lindekens-at-yahoo.com Fri Mar 13 03:42:53 2009 12, 20 -- Received: from web110205.mail.gq1.yahoo.com (web110205.mail.gq1.yahoo.com [67.195.8.181]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2D8grtn013718 12, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 03:42:53 -0500 12, 20 -- Received: (qmail 96143 invoked by uid 60001); 13 Mar 2009 08:42:53 -0000 12, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1236933772; bh=u3PFN5xmAeKkKwc9qU/XePpTSqt40gOyJRvzDzTYkmA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=DSLN1v/n99pjlNJQWlMys5OqOKPRikFg/9N0eAhZpBNGFPgxacNMsBUEhy/BIJnA2T2qk3vmsaiaVesLsUT3I/UGAtGXO3swIe0rx8czto8gaV/9Q0OgtR2naHQOIpSzHozoZQBYV2FYhrB1cyDN1/nuSxWnebBwFVKtFL6GAnc= 12, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 20 -- s=s1024; d=yahoo.com; 12, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; 12, 20 -- b=ppbdKNUOa8R3S4Z5mN/xrT3MnYKg+MxcyxIp5WZhJKba25k33Y0Lcm35gAr6JzISt+NXpU5O/wrnQdEPNwpt0qxK8ppYCvPD5gv5Jq33ZbLoDPzNFjRN1380Qq34a1n4GK2u/yXN3lV+9QNqiXbFHzdDYEfhD70Gszs5jpiXSKY=; 12, 20 -- Message-ID: {910041.94555.qm-at-web110205.mail.gq1.yahoo.com} 12, 20 -- X-YMail-OSG: Mpcrg.EVM1nQMDZ5tr3RBagB0jL8I9fNt9QFWV0NKIAGPvsP7PfJfbrcFdmx7pyeGFpzN1P5gYoTBBQuGawFytvwWerSoojgKaMaUU3D6_XCPQ3UETfnXXwWjZg4Xat4IWwPliFyLxi0aaQSo4Oo7FW_YUV2QGfarqJkzSA5ZI6rwVPOVni6.GfUmoSBh3IYp8TAJqEgxKlj.Ird2uJcbapDSqGpGUcxnw9tswcKSzwq0YcjxZaNAMMXGJ3D0gwrYSPP1a7lFToodNio_9EcKuzX0BGCFiev1Ba7auTSZpZ5dy4hQGeRtr1Ukcvg2ffja3IQBfovI3sRDoajp5JyUYs- 12, 20 -- Received: from [81.243.51.106] by web110205.mail.gq1.yahoo.com via HTTP; Fri, 13 Mar 2009 01:42:52 PDT 12, 20 -- X-Mailer: YahooMailWebService/0.7.289.1 12, 20 -- Date: Fri, 13 Mar 2009 01:42:52 -0700 (PDT) 12, 20 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com} 12, 20 -- Subject: Cleaning and lubricating a Jung Tetrander and a Leitz 1300 sledge microtome ??? 12, 20 -- To: Microscopy-at-microscopy.com 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
I'm sorry but I have to take you to task about climbers at and above 6.5 km. First of all THEY MUST ACCLIMATIZE usually by walking there and secondly a significant number of people have problems with the reduced levels of oxygen at those altitudes. If you took the best mountain climbers in the world and dropped them (gently) on Mount Everest without acclimatizing they would be dead in minutes. Similarly I think with levels of oxygen below about 16% for most people.
Perhaps if people equated a low oxygen level with the mountaineer's "Death Zone" it would put things into perspective.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: tivol-at-caltech.edu
Samuel beat me to a reply yonks ago, but anyway:
A high frame rate low noise fluorescence camera, that's kind of asking a lot. The Photometrics Evolve EMCCD camera has a frame rate of 30 fps at 1x binning and is suitable for low-noise fluorescence microscopy imaging. But even at 1x binning it's only 512x512 pixels [fast capture and zilch noise though]. Plus don't expect much change from £25k for many EMCCD cameras. Not sure there's anything camera wise that’s suitable for low noise fluorescence that can take say 5MP frame rates at video frequency.
About 2 times a second [time-lapse] is the standard rate from cheaper low noise fluorescence cameras like our [old] £8k Peltier cooled Hamamatsu Orca 100, capturing at 1xbinning ~1MP [1344x1024]. Dropping to 2xbinning will half the pixel resolution by combining 4 pixels into 1 [with the Orca], but it will double sensitivity [plus there is the gain control], but this won't get you close to video rates. It depends how bright your fluorescence is and how much you can put up with image noise or low resolution and thus poor image quality though - megapixels aren't everything in low light situations [just try it with your 13MP digital SLR]. Plus it depends on how much money you have. Of course camera technology is moving at a fast pace and our Hamamatsu Orca 100 fluorescence camera has less sensitivity and resolution that more recent Hamamatsu type interline models [although the new cameras will either have more sensitivity or more pixels, as an increase in one generally reduces the other].
Spinning disk confocals also offer very fast frame rates [as they come with expensive Photometrics 30fps EMCCD or 15fps Interline CCD type cameras or similar modern Hamamatsu Orcas], so if you know someone friendly who has one nearby...
Following your additional post:
Fast frame rate and bright field is no problem, get a cheap[ish] 1MP or so video camera, there's plenty of light as you can just turn up the halogen light bulb. Low light fluorescence, no problem, get an expensive low noise 1MP or so B&W cooled fluorescence camera. Quality hi-res bright field colour photo images, no problem get a decent low frame rate 5MP CCD colour camera. Total price, well a lot, from £10k upwards.
This is in fact what we did with our PALM laser dissection system, three cameras: Zeiss Video, Zeiss MRCm [fluorescence B&W] and Zeiss MRCm [colour], all controlled by one software package [Axiovision]. All supplied by Zeiss though, and as part of the expensive PALM system bid [when with the generous discount they almost came 'free']. Other Systems here generally have both low frame rate 5MP colour CCD cameras and peltier cooled 1MP B&W fluorescence cameras [so we do time-lapse acquisition rather than video] - image capture/analysis software by MetaMorph, IPLabs and NIS Elements. You can get Interline CCD colour cameras that can do binning to increase sensitivity [like their B&W cousins] but the resolution halves with each binning factor [never tried them - ours rely on very noisy gain for low light, so they are no good for anything other than dazzling fluorescence - great for transmitted light phase and stained sections though].
There are third party suppliers for those on a budget, but there's no single ideal camera for your needs [or anybody else's probably]. If you need fast frame rates of 30 fps, then that is the deciding factor for a camera choice. Otherwise a low noise cooled B&W time-lapse fluorescence camera seems your best option, as it works well for fluorescence and DIC/Ph transmitted light and you just increase resolution by changing the objective or via a 1.5x optical zoom [if fitted].
I've ignored things like cameras spectral sensitivity, but that can be relevant, e.g. in respect to far-red imaging.
I expect you know all this really, but that perfect camera [also giving change from £3,000] sadly doesn't exist.
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: ben.micklem-at-pharm.ox.ac.uk [mailto:ben.micklem-at-pharm.ox.ac.uk] Sent: 09 March 2009 12:24 To: kjmorris-at-well.ox.ac.uk
I was wondering whether anyone has a high frame rate (20 to 30 fps at full resolution) monochrome camera they can recommend for fluorescence microscopy, with a resolution of at least 2 megapixels?
It seems there are a few around 1 megapixel, but it is hard to find anything higher resolution.
Regards,
Ben
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 {http://mrcanu.pharm.ox.ac.uk/}
==============================Original Headers============================== 8, 26 -- From ben.micklem-at-pharm.ox.ac.uk Mon Mar 9 07:10:56 2009 8, 26 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n29CAuE5004150 8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Mar 2009 07:10:56 -0500 8, 26 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 8, 26 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69) 8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 8, 26 -- id 1LgeK1-0004Lo-TN 8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000 8, 26 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) 8, 26 -- by smtp2.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 8, 26 -- (Exim 4.69) 8, 26 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 8, 26 -- id 1LgeK1-0004xU-6Z 8, 26 -- for microscopy-at-microscopy.com; Mon, 09 Mar 2009 12:11:05 +0000 8, 26 -- Message-ID: {49B50758.9070309-at-pharm.ox.ac.uk} 8, 26 -- Date: Mon, 09 Mar 2009 12:11:04 +0000 8, 26 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 8, 26 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 8, 26 -- MIME-Version: 1.0 8, 26 -- To: microscopy-at-microscopy.com 8, 26 -- Subject: Fast monochrome digital camera 8, 26 -- X-Enigmail-Version: 0.95.7 8, 26 -- Content-Type: text/plain; charset=ISO-8859-1 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- X-Oxford-Username: phar0293 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 23 -- From kjmorris-at-well.ox.ac.uk Fri Mar 13 05:28:08 2009 29, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 29, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DAS7Fo015528 29, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 05:28:07 -0500 29, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 29, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 29, 23 -- id 1Li4cZ-0001DC-Ds 29, 23 -- for microscopy-at-microscopy.com; Fri, 13 Mar 2009 10:28:07 +0000 29, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 29, 23 -- To: {microscopy-at-microscopy.com} 29, 23 -- References: {200903091224.n29CO8nX015042-at-ns.microscopy.com} 29, 23 -- Subject: RE: [Microscopy] Fast monochrome digital camera 29, 23 -- Date: Fri, 13 Mar 2009 10:28:08 -0000 29, 23 -- Message-ID: {6070AFFE2A2244648963F54F48919BF9-at-CytoWhizz} 29, 23 -- MIME-Version: 1.0 29, 23 -- Content-Type: text/plain; 29, 23 -- charset="iso-8859-1" 29, 23 -- X-Mailer: Microsoft Office Outlook 11 29, 23 -- In-Reply-To: {200903091224.n29CO8nX015042-at-ns.microscopy.com} 29, 23 -- Thread-Index: AcmgsgN+BXsFSGZZRYKUXEWb+3xvHgBnRGsg 29, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 29, 23 -- Content-Transfer-Encoding: 8bit 29, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DAS7Fo015528 ==============================End of - Headers==============================
10th Inter-American Congress of Electron Microscopy, 2009 Rosario, Argentina
October 25-28 2009
Every two years, CIASEM - the microscopy organization for the western hemisphere - holds the principal regional meeting on microscopy. The next meeting in the series will be held in Rosario, Argentina. The dates are October 25-28 2009.
This reminder is to let you know that the date for submission of abstracts is just a week away: March 20, 2009. If this sounds like not enough time, let me draw to your attention that the abstract required now is not the normal two-page abstract, but is a 200-word short abstract. The extended two-page abstract is not due until June.
These are exciting meetings. I would like to encourage you to attend.
Full information is available at the Congress web site: http://www.ciasem2009.com.ar/
The site for CIASEM in general (which includes, for example, the proceedings of the previous meeting) is: http://www.ciasem.com/
Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 11, 21 -- From jae5-at-lehigh.edu Fri Mar 13 10:46:36 2009 11, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DFkZ39017191 11, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 10:46:35 -0500 11, 21 -- Received: from [127.0.0.1] (r054059.mat.Lehigh.EDU [128.180.54.59]) 11, 21 -- (authenticated bits=0) 11, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id n2DFkXEt007074 11, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 11:46:34 -0400 11, 21 -- Message-ID: {49BA7FD9.5010000-at-lehigh.edu} 11, 21 -- Date: Fri, 13 Mar 2009 11:46:33 -0400 11, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 11, 21 -- Organization: Lehigh University 11, 21 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 11, 21 -- MIME-Version: 1.0 11, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 11, 21 -- Subject: Inter-American meeting on microscopy, Rosario, Argentina, next October 11, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 21 -- Content-Transfer-Encoding: 7bit 11, 21 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on rain.cc.lehigh.edu 11, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
We have our LN2 filling station in a smallish room off our loading dock with an O2 sensor/alarm. With the doors open and a fan moving the cold N2 vapours out off the floor, O2 levels will drop from 20.8% (by the meter read-out) to 20.1% when filling a cold 10L dewar
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Friday, March 13, 2009 5:29 AM To: rjharris-at-uwo.ca
Bill
I'm sorry but I have to take you to task about climbers at and above 6.5 km. First of all THEY MUST ACCLIMATIZE usually by walking there and secondly a significant number of people have problems with the reduced levels of oxygen at those altitudes. If you took the best mountain climbers in the world and dropped them (gently) on Mount Everest without acclimatizing they would be dead in minutes. Similarly I think with levels of oxygen below about 16% for most people.
Perhaps if people equated a low oxygen level with the mountaineer's "Death Zone" it would put things into perspective.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: tivol-at-caltech.edu
I have not seen high resolution b/w or color cameras that are that fast either. Depending on why one would want a high frame rate, you might consider:
It is 1.5M pixels, 0.002Lux sensitivity and 30fps at 640x480 but noiseless capture at 1.5M pixels. Last time I sold one, they were about $4500US. For most work, high pixel count is not necessary. I have two new Penguin 150CL cameras as residual units that I don't need. If anyone is interested in these, they can contact me off-line.
gary g.
At 03:29 AM 3/13/2009, you wrote:
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==============================Original Headers============================== 11, 22 -- From gary-at-gaugler.com Fri Mar 13 13:17:23 2009 11, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2DIHJsh019267 11, 22 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 13:17:21 -0500 11, 22 -- Message-Id: {200903131817.n2DIHJsh019267-at-ns.microscopy.com} 11, 22 -- Received: (qmail 26321 invoked from network); 13 Mar 2009 11:22:52 -0700 11, 22 -- Received: by simscan 1.1.0 ppid: 26318, pid: 26319, t: 0.1448s 11, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 22 -- by smtp1 with SMTP; 13 Mar 2009 11:22:52 -0700 11, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 22 -- Date: Fri, 13 Mar 2009 11:17:01 -0700 11, 22 -- To: kjmorris-at-well.ox.ac.uk 11, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 22 -- Subject: Re: [Microscopy] RE: Fast monochrome digital camera 11, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 22 -- In-Reply-To: {200903131029.n2DATXAE017919-at-ns.microscopy.com} 11, 22 -- References: {200903131029.n2DATXAE017919-at-ns.microscopy.com} 11, 22 -- Mime-Version: 1.0 11, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DIHJsh019267 ==============================End of - Headers==============================
Hi All, I am looking at purchasing a Phenom SEM by FEI. Does anyone have experience with this instrument and what do you think? I'd be using it for confirming diatom identifications. Thanks, Ann.
-- Ann St. Amand, Ph.D., CLP President PhycoTech, Inc 620 Broad St., Suite 100 St. Joseph, MI 49085
Larry; You raise another point about hypoxia: high altitude pilots are trained to recognize the symptoms. The symptoms are so subtle, most people don't recognize them and don't know they are about to pass out. Interestingly, this was first recognized shortly after WW2 by Charles Lindberg when he was working as a test pilot in the new jet fighters.
John Mardinly, Numonyx
-----Original Message----- X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu] Sent: Thursday, March 12, 2009 3:25 PM To: MARDINLY, A
Sometimes good intentions go awry as well. Some years ago when a freezer
or dewar failed a lab person stored items in a portable dewar and then placed it in a cold room. It cold in there and should last longer--right? Later someone else entered the room and nearly passed out
due to the lack of oxygen. He was smart and had experience at high altitudes so managed to get out and survive. Whew! Larry
jkrupp-at-deltacollege.edu wrote: } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } } You should also not discount the possibility of a pressure relief } valve failure or poor insulation causing higher than normal boil off. } } I once had a tank's high pressure safety go off. Sounded like a jet } plane and it filled the room with nitrogen gas in a matter of minutes.
} Called the fire department and they advised us to evacuate the room } and go outside. The came screaming up with full breathing apparat. and
} checked the room for us before we went back in. No harm done, but } pretty exciting. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original Headers============================== } 8, 44 -- From jkrupp-at-deltacollege.edu Thu Mar 12 16:55:18 2009 } 8, 44 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 8, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2CLt5iP026014 } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 16:55:18 -0500 } 8, 44 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 8, 44 -- by localhost (Email Security Appliance) with SMTP id CB0691B9464_9B97F17B } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:03 +0000 (GMT) } 8, 44 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) } 8, 44 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 8C8E3183B0B_9B97F17F } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 21:31:01 +0000 (GMT) } 8, 44 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) } 8, 44 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) } 8, 44 -- with ESMTP id 46042684 for microscopy-at-microscopy.com; Thu, 12 Mar 2009 14:54:11 -0700 } 8, 44 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by } 8, 44 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 8, 44 -- ESMTP id KGEXEU00.2M0 for {microscopy-at-microscopy.com} ; Thu, 12 } 8, 44 -- Mar 2009 14:37:42 -0700 } 8, 44 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 0F9BA8F7501D } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:11 -0700 (PDT) } 8, 44 -- X-Virus-Scanned: amavisd-new at } 8, 44 -- X-Spam-Flag: NO } 8, 44 -- X-Spam-Score: -2.499 } 8, 44 -- X-Spam-Level: } 8, 44 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, } 8, 44 -- BAYES_00=-2.599, RDNS_NONE=0.1] } 8, 44 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 8, 44 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 8, 44 -- with ESMTP id nPmPguQidFlT for {microscopy-at-microscopy.com} ; } 8, 44 -- Thu, 12 Mar 2009 14:54:10 -0700 (PDT) } 8, 44 -- Received: from [172.20.3.146] (unknown [172.20.3.146]) } 8, 44 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 2AE0A8F7501E } 8, 44 -- for {microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 14:54:10 -0700 (PDT) } 8, 44 -- Message-Id: {21D383E5-4DE8-4383-BA34-3F1210F08BA5-at-deltacollege.edu} } 8, 44 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 8, 44 -- To: microscopy-at-microscopy.com } 8, 44 -- In-Reply-To: {200903122026.n2CKQYmD021645-at-ns.microscopy.com} } 8, 44 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 8, 44 -- Content-Transfer-Encoding: 7bit } 8, 44 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 8, 44 -- Subject: Re: [Microscopy] nitrogen supply at the microscope } 8, 44 -- Date: Thu, 12 Mar 2009 14:54:09 -0700 } 8, 44 -- References: {200903122026.n2CKQYmD021645-at-ns.microscopy.com} } 8, 44 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers============================== }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 6, 41 -- From Larry.Ackerman-at-ucsf.edu Thu Mar 12 17:19:22 2009 6, 41 -- Received: from emfmcb01.ucsfmedicalcenter.org (EMFMCB01.ucsfmedicalcenter.org [64.54.46.97]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2CMJLWJ008140 6, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 17:19:22 -0500 6, 41 -- Received: from [64.54.35.209] by emfmcb01.ucsfmedicalcenter.org with 6, 41 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2)); 6, 41 -- Thu, 12 Mar 2009 15:19:17 -0700 6, 41 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B 6, 41 -- X-AuditID: 403623d1-a7451bb00000708a-90-49b98a655eb0 6, 41 -- Received: from EXHT02.net.ucsf.edu (unknown [64.54.247.219]) by 6, 41 -- vsobmcb01.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id 6, 41 -- 27FF715C7 for {Microscopy-at-microscopy.com} ; Thu, 12 Mar 2009 15:19:17 6, 41 -- -0700 (PDT) 6, 41 -- Received: from EXVS05.net.ucsf.edu (64.54.128.150) by 6, 41 -- EXHT02.net.ucsf.edu (64.54.247.219) with Microsoft SMTP Server id 6, 41 -- 8.1.340.0; Thu, 12 Mar 2009 15:19:16 -0700 6, 41 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by 6, 41 -- EXVS05.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 12 Mar 6, 41 -- 2009 15:18:29 -0700 6, 41 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 6, 41 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 12 Mar 6, 41 -- 2009 15:18:28 -0700 6, 41 -- Message-ID: {49B98A34.2010006-at-ucsf.edu} 6, 41 -- Date: Thu, 12 Mar 2009 15:18:28 -0700 6, 41 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 6, 41 -- Reply-to: larry.ackerman-at-ucsf.edu 6, 41 -- Organization: UCSF, NeuroAnatomy 6, 41 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 6, 41 -- MIME-Version: 1.0 6, 41 -- To: Microscopy-at-microscopy.com 6, 41 -- Subject: Re: [Microscopy] Re: nitrogen supply at the microscope 6, 41 -- References: {200903122203.n2CM3spi003857-at-ns.microscopy.com} 6, 41 -- In-Reply-To: {200903122203.n2CM3spi003857-at-ns.microscopy.com} 6, 41 -- X-OriginalArrivalTime: 12 Mar 2009 22:18:28.0719 (UTC) 6, 41 -- FILETIME=[784BC7F0:01C9A360] 6, 41 -- X-Brightmail-Tracker: AAAAAQ3stXE= 6, 41 -- X-WSS-ID: 65A755EF1O868741-01-01 6, 41 -- Content-Type: text/plain; 6, 41 -- charset=iso-8859-1; 6, 41 -- format=flowed 6, 41 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 27 -- From A.MARDINLY-at-numonyx.com Fri Mar 13 15:30:02 2009 19, 27 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DKU2Mb019096 19, 27 -- for {Microscopy-at-Microscopy.com} ; Fri, 13 Mar 2009 15:30:02 -0500 19, 27 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 19, 27 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 6BBFA144004 19, 27 -- for {Microscopy-at-Microscopy.com} ; Fri, 13 Mar 2009 14:32:35 -0400 (EDT) 19, 27 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 19, 27 -- Fri, 13 Mar 2009 16:30:01 -0400 19, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 27 -- Content-class: urn:content-classes:message 19, 27 -- MIME-Version: 1.0 19, 27 -- Content-Type: text/plain; 19, 27 -- charset="us-ascii" 19, 27 -- Subject: RE: [Microscopy] nitrogen supply at the microscope 19, 27 -- Date: Fri, 13 Mar 2009 16:30:00 -0400 19, 27 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39D87-at-EXDRESBENMX012.numonyx.local} 19, 27 -- X-MS-Has-Attach: 19, 27 -- X-MS-TNEF-Correlator: 19, 27 -- Thread-Topic: [Microscopy] nitrogen supply at the microscope 19, 27 -- Thread-Index: AcmjYVt1LO2mWcXsSJWOUp2KYteyawAuFvywAAAuocA= 19, 27 -- References: {200903122224.n2CMOhut019527-at-ns.microscopy.com} 19, 27 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 19, 27 -- To: {Microscopy-at-Microscopy.com} 19, 27 -- X-OriginalArrivalTime: 13 Mar 2009 20:30:01.0830 (UTC) FILETIME=[7C4D1060:01C9A41A] 19, 27 -- Content-Transfer-Encoding: 8bit 19, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DKU2Mb019096 ==============================End of - Headers==============================
We have one and we're very happy with it. The ease of use is outstanding, and the depth-of-field we can achieve is extremely useful. FEI's customer service is world-class, as well. We have used it to look at diatoms with good results (we use them for filters).
I am finding, however, that once you have a SEM, you start seeing a "need" for many other pieces of equipment that you might never have acquired otherwise (sputter coaters, microtomes, diamond saws, etc.) It is also not a particularly quiet instrument. Although I have minimal experience with other EM's to compare it to, it's much noisier than my light microscope.
I have no connection to FEI other than as a satisfied customer.
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
-----Original Message----- X-from: astamand-at-phycotech.com [mailto:astamand-at-phycotech.com] Sent: Friday, March 13, 2009 1:32 PM To: Zonis, Robert
Poor Charles Lindbergh; he is credited with so much that he never did.... The first high altitude studies of hypoxia (not counting anecdotal reports from early mountain climbers-- remember poor Ötzi) were conducted by the British Association for the Advancement of Science in the 1850's (yes, 19th century) using balloons. The first serious encounter with hypoxia by a balloonist occurred in 1804, by one Joseph Louis Gay Lussac, who made several flights above 20,000 feet/6000 meters. I am hardly an expert in aviation (or anything else for that matter) but in the age of Google, I have a compulsion for checking facts before going public....
Cheers Roger
} From: A.MARDINLY-at-numonyx.com } Reply-To: A.MARDINLY-at-numonyx.com } Date: Fri, 13 Mar 2009 15:38:20 -0500 } To: raristau-at-ims.uconn.edu } Subject: [Microscopy] RE: nitrogen supply at the microscope } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Larry; } You raise another point about hypoxia: high altitude pilots are } trained to recognize the symptoms. The symptoms are so subtle, most } people don't recognize them and don't know they are about to pass out. } Interestingly, this was first recognized shortly after WW2 by Charles } Lindberg when he was working as a test pilot in the new jet fighters. } } John Mardinly, } Numonyx }
==============================Original Headers============================== 5, 18 -- From raristau-at-ims.uconn.edu Fri Mar 13 16:17:08 2009 5, 18 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DLH7RZ004625 5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 16:17:07 -0500 5, 18 -- Received: from [137.99.19.228] (d19h228.public.uconn.edu [137.99.19.228]) 5, 18 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n2DLH7YJ025106 5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 17:17:07 -0400 5, 18 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 18 -- Date: Fri, 13 Mar 2009 17:14:30 -0400 5, 18 -- Subject: RE: nitrogen supply at the microscope 5, 18 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 5, 18 -- To: {microscopy-at-microscopy.com} 5, 18 -- Message-ID: {C5E044F6.48B1%raristau-at-ims.uconn.edu} 5, 18 -- In-Reply-To: {200903132038.n2DKcK9B007717-at-ns.microscopy.com} 5, 18 -- Mime-version: 1.0 5, 18 -- Content-type: text/plain; charset="ISO-8859-1" 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DLH7RZ004625 ==============================End of - Headers==============================
Roger; Maybe I should have clarified the hypoxia description as rapid onset-something mountain climbers and ballooners never experienced. My reference is the book "Lindbergh" by A. Scott Berg, Putnams, NY, 1998. Starting on Page 446, there is a description of work Lindbergh did with Dr. Walter M. Boothby at the Mayo Clinic in 1942. Both were concerned with rapid changes in pressure and oxygen at the 40,000 foot level, situations never encountered by man until the onset of high performance aircraft. Initial testing was done over a 10 day period in a ground based chamber capable of rapid evacuation. During that time. Lindbergh challenged the prevailing opinion that no one could be trained to recognize the rapid onset of hypoxia to such an extent that they could do anything about it. Subsequent testing was done in P-47 aircraft. One situation occurred in which his oxygen tank ran out at the same time an altimeter malfunctioned and he recognized the symptoms and put the plane into a steep dive as he lost consciousness gave credence to his theory. So Roger, I love Google too, just read a book every once and a while. For microscopists, the lesson is that they never undergo training to recognize hypoxia, and a sudden release of nitrogen or just walking into a room with the oxygen level below what will sustain consciousness is extremely dangerous. They will just conk out, rather than run for the exit.
John Mardinly, Numonyx
-----Original Message----- X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] Sent: Friday, March 13, 2009 2:23 PM To: MARDINLY, A
Poor Charles Lindbergh; he is credited with so much that he never did.... The first high altitude studies of hypoxia (not counting anecdotal reports from early mountain climbers-- remember poor Ötzi) were conducted by the British Association for the Advancement of Science in the 1850's (yes, 19th century) using balloons. The first serious encounter with hypoxia by a balloonist occurred in 1804, by one Joseph Louis Gay Lussac, who made several flights above 20,000 feet/6000 meters. I am hardly an expert in aviation (or anything else for that matter) but in the age of Google, I have a compulsion for checking facts before going public....
Cheers Roger
} From: A.MARDINLY-at-numonyx.com } Reply-To: A.MARDINLY-at-numonyx.com } Date: Fri, 13 Mar 2009 15:38:20 -0500 } To: raristau-at-ims.uconn.edu } Subject: [Microscopy] RE: nitrogen supply at the microscope } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Larry; } You raise another point about hypoxia: high altitude pilots are } trained to recognize the symptoms. The symptoms are so subtle, most } people don't recognize them and don't know they are about to pass out. } Interestingly, this was first recognized shortly after WW2 by Charles } Lindberg when he was working as a test pilot in the new jet fighters. } } John Mardinly, } Numonyx }
==============================Original Headers============================== 5, 18 -- From raristau-at-ims.uconn.edu Fri Mar 13 16:17:08 2009 5, 18 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2DLH7RZ004625 5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 16:17:07 -0500 5, 18 -- Received: from [137.99.19.228] (d19h228.public.uconn.edu [137.99.19.228]) 5, 18 -- by mail2.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id n2DLH7YJ025106 5, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Mar 2009 17:17:07 -0400 5, 18 -- User-Agent: Microsoft-Entourage/10.1.4.030702.0 5, 18 -- Date: Fri, 13 Mar 2009 17:14:30 -0400 5, 18 -- Subject: RE: nitrogen supply at the microscope 5, 18 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 5, 18 -- To: {microscopy-at-microscopy.com} 5, 18 -- Message-ID: {C5E044F6.48B1%raristau-at-ims.uconn.edu} 5, 18 -- In-Reply-To: {200903132038.n2DKcK9B007717-at-ns.microscopy.com} 5, 18 -- Mime-version: 1.0 5, 18 -- Content-type: text/plain; charset="ISO-8859-1" 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2DLH7RZ004625 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 29 -- From A.MARDINLY-at-numonyx.com Sun Mar 15 04:33:30 2009 15, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2F9XUZd024538 15, 29 -- for {Microscopy-at-Microscopy.com} ; Sun, 15 Mar 2009 04:33:30 -0500 15, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 15, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 9CBCF4143C0; 15, 29 -- Sun, 15 Mar 2009 04:51:02 -0400 (EDT) 15, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 15, 29 -- Sun, 15 Mar 2009 05:33:29 -0400 15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 29 -- Content-class: urn:content-classes:message 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; 15, 29 -- charset="iso-8859-1" 15, 29 -- Subject: RE: [Microscopy] RE: nitrogen supply at the microscope 15, 29 -- Date: Sun, 15 Mar 2009 05:33:27 -0400 15, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F39E27-at-EXDRESBENMX012.numonyx.local} 15, 29 -- In-Reply-To: {200903132123.n2DLNNVq017094-at-ns.microscopy.com} 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- Thread-Topic: [Microscopy] RE: nitrogen supply at the microscope 15, 29 -- Thread-Index: AcmkIfS2NgDLQbZLSpKo+n/noBg2QQBKvWGw 15, 29 -- References: {200903132123.n2DLNNVq017094-at-ns.microscopy.com} 15, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 15, 29 -- To: {raristau-at-ims.uconn.edu} 15, 29 -- Cc: {Microscopy-at-Microscopy.com} 15, 29 -- X-OriginalArrivalTime: 15 Mar 2009 09:33:29.0546 (UTC) FILETIME=[197F0AA0:01C9A551] 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2F9XUZd024538 ==============================End of - Headers==============================
There have been a lot of comments on the dangers of hypoxia from leaking or defective liquid nitrogen tanks. All very interesting.
QUESTION: Are there any straightforward symptoms of the onset of hypoxia that can be recognized in time to avoid succumbing to the situation?? -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 14 -- From bigelow-at-umich.edu Sun Mar 15 12:12:54 2009 2, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FHCqTn025128 2, 14 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:12:53 -0500 2, 14 -- Received: FROM [76.234.131.113] (adsl-76-234-131-113.dsl.sfldmi.sbcglobal.net [76.234.131.113]) 2, 14 -- BY hellskitchen.mr.itd.umich.edu ID 49BD3711.9A774.25996 ; 2, 14 -- 15 Mar 2009 13:12:50 -0400 2, 14 -- Mime-Version: 1.0 2, 14 -- Message-Id: {p06240800c5e2e6aed43a-at-[99.130.26.88]} 2, 14 -- Date: Sun, 15 Mar 2009 13:12:46 -0400 2, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 14 -- Subject: [Microscopy] RE: symptoms of hypoxia 2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I'm not certain of the official guidelines for symptoms, but they may be similar to asthma, as it's also a condition of low O2 when flared.
__ blacking out, or vision seeming to de-pixil some.
__ Closing one's eyes after seeing a contrasted object, and still retaining the image for longer than normal when the eyes are closed.
__Slurred speech & slowed thinking
__ sometimes some dyslexia ( when I start mixing words it's time for the inhaler)
__ sometimes even stumbling on your own feet.
__fatigue, that goes away when you are not near the trigger
__ Nausea, vertigo
Lou Ann
bigelow-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } There have been a lot of comments on the dangers of hypoxia from } leaking or defective liquid nitrogen tanks. All very interesting. } } QUESTION: Are there any straightforward symptoms of the onset of } hypoxia that can be recognized in time to avoid succumbing to the } situation?? }
==============================Original Headers============================== 16, 21 -- From lamiller-at-illinois.edu Sun Mar 15 12:41:43 2009 16, 21 -- Received: from QMTA04.emeryville.ca.mail.comcast.net (qmta04.emeryville.ca.mail.comcast.net [76.96.30.40]) 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FHfgCP007104 16, 21 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:41:42 -0500 16, 21 -- Received: from OMTA04.emeryville.ca.mail.comcast.net ([76.96.30.35]) 16, 21 -- by QMTA04.emeryville.ca.mail.comcast.net with comcast 16, 21 -- id TTmD1b0070lTkoCA4Vhjvq; Sun, 15 Mar 2009 17:41:43 +0000 16, 21 -- Received: from lou-anns-computer.local ([98.212.148.206]) 16, 21 -- by OMTA04.emeryville.ca.mail.comcast.net with comcast 16, 21 -- id TVhh1b0044TRewa8QVhhUW; Sun, 15 Mar 2009 17:41:42 +0000 16, 21 -- Message-ID: {49BD3DD3.9050402-at-illinois.edu} 16, 21 -- Date: Sun, 15 Mar 2009 12:41:39 -0500 16, 21 -- From: Lou Ann Miller {lamiller-at-illinois.edu} 16, 21 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 16, 21 -- MIME-Version: 1.0 16, 21 -- To: bigelow-at-umich.edu, microscopy-at-microscopy.com 16, 21 -- Subject: Re: [Microscopy] RE: symptoms of hypoxia 16, 21 -- References: {200903151729.n2FHTKUa004625-at-ns.microscopy.com} 16, 21 -- In-Reply-To: {200903151729.n2FHTKUa004625-at-ns.microscopy.com} 16, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Personally, I would like this thread to turn away from "hypoxia", the history, or symptoms.
The MSDS, fire code, etc.... clearly state "do not store LN2 is confined spaces". Additionally, there must be excellent ventilation.
Some suggest to wear a self-contained breathing apparatus if the oxygen content routinely falls below 19%.
In New York City, you cannot legally keep a dewar within a few feet of a door or exit. Infact, it is illegal there to store LN2 is a public hallway.
Hence, let us be preventive. An oxygen monitor from MSA (Mine Safety Appliances) can be bought from Lab Safety Supply, Fisher Sci, etc... for just $200.
regards,
JQuinn
==============================Original Headers============================== 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sun Mar 15 13:59:21 2009 11, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FIxLPu022978 11, 12 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 13:59:21 -0500 11, 12 -- Received: (from jquinn-at-localhost) 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id n2FJwrj16350 11, 12 -- for microscopy-at-microscopy.com; Sun, 15 Mar 2009 14:58:53 -0500 11, 12 -- Date: Sun, 15 Mar 2009 14:58:53 -0500 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 11, 12 -- Message-Id: {200903151958.n2FJwrj16350-at-www.matscieng.sunysb.edu} 11, 12 -- To: microscopy-at-microscopy.com 11, 12 -- Subject: re: LN2 in confined space ==============================End of - Headers==============================
} Hi All, I am looking at purchasing a Phenom SEM by FEI. Does } anyone have experience with this instrument and what do you } think? I'd be using it for confirming diatom } identifications. Thanks, Ann.
It seems to me this countertop SEM images with backscattered electrons only, which may be a bit contrasty for imaging extremely 3D subjects (eg, diatoms). Have FEI image a couple of your samples for you.
hth, Michael Shaffer :o)
SEM/MLA Research Coordinator http://www.mun.ca/creait/maf/ Inco Innovation Centre Memorial University St. John's, Newfoundland
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==============================Original Headers============================== 10, 29 -- From michael-at-shaffer.net Mon Mar 16 04:55:02 2009 10, 29 -- Received: from smtprelay.hostedemail.com (smtprelay0139.hostedemail.com [216.40.44.139]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2G9t29q016055 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 04:55:02 -0500 10, 29 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 10, 29 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id A57EF1DDDA04; 10, 29 -- Mon, 16 Mar 2009 09:55:01 +0000 (UTC) 10, 29 -- X-SpamScore: 1 10, 29 -- X-Spam-Summary: 50,0,0,0839f5eead2c7a27,0d355e4b7ffc0630,michael-at-shaffer.net,astamand-at-phycotech.com:microscopy-at-microscopy.com,RULES_HIT:10:355:379:539:541:542:599:601:945:967:973:988:989:1155:1160:1260:1277:1311:1313:1314:1345:1359:1437:1515:1516:1518:1534:1540:1593:1594:1711:1730:1747:1766:1792:2234:2378:2393:2525:2553:2560:2564:2682:2685:2857:2859:2892:2933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3352:3636:3770:3865:3866:3867:3868:3869:3872:3873:3876:3877:3934:3936:3938:3941:3944:3947:3950:3953:3956:3959:4250:4321:4659:4699:4860:5007:6114:6261:7679:7903:8501:8985:9025:9388,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 10, 29 -- Received: from rarewolf (CPE001d7e3e1515-CM0018682b4b4c.cpe.net.cable.rogers.com [72.139.95.151]) 10, 29 -- (Authenticated sender: michael-at-shaffer.net) 10, 29 -- by omf06.hostedemail.com (Postfix) with ESMTP; 10, 29 -- Mon, 16 Mar 2009 09:55:01 +0000 (UTC) 10, 29 -- From: "michael shaffer" {michael-at-shaffer.net} 10, 29 -- To: {astamand-at-phycotech.com} 10, 29 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 10, 29 -- References: {200903131828.n2DIS7OM001444-at-ns.microscopy.com} 10, 29 -- Subject: RE: [Microscopy] SEM 10, 29 -- Date: Mon, 16 Mar 2009 07:24:58 -0230 10, 29 -- Message-ID: {5EF33F2F45F24A34B82694993BCCC84F-at-rarewolf} 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; 10, 29 -- charset="US-ASCII" 10, 29 -- Content-Transfer-Encoding: 7bit 10, 29 -- X-Mailer: Microsoft Office Outlook 11 10, 29 -- In-Reply-To: {200903131828.n2DIS7OM001444-at-ns.microscopy.com} 10, 29 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 10, 29 -- Thread-Index: AcmkCXTru/iPA2+uRMWLGmDDqSCXYwCE1CdA 10, 29 -- X-session-marker: 6D69636861656C40736861666665722E6E6574 ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society will hold its first meeting of 2009 on Friday, March 27th, at Northwestern University in Evanston, IL. Our theme is "Greater than the Sum: Advances in Correlative Microscopy". Program details and regstration information can be found on our website under Meetings:
www.midwestmicroscopy.org
Please join us to hear an excellent group of speakers and to show support for your Local Affiliate Society as we kick off another year of activities!
Elaine Schumacher M3S Program Coodinator
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com} Web Site: www.mccrone.com {http://www.mccrone.com/}
==============================Original Headers============================== 12, 23 -- From eschumacher-at-mccrone.com Mon Mar 16 08:14:08 2009 12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GDE5bo017372 12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 08:14:07 -0500 12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 12, 23 -- Mon, 16 Mar 2009 08:14:05 -0500 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="us-ascii" 12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society 12, 23 -- Date: Mon, 16 Mar 2009 08:13:18 -0500 12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A702089952-at-MCCRONEMSG.tmg.mccrone.com} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society 12, 23 -- Thread-Index: AcmmOPjtmFFHfYRJSlKcMJ0caaMIgQ== 12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 23 -- To: {microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 16 Mar 2009 13:14:05.0664 (UTC) FILETIME=[15401E00:01C9A639] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GDE5bo017372 ==============================End of - Headers==============================
Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158
-----Original Message----- X-from: lamiller-at-illinois.edu [mailto:lamiller-at-illinois.edu] Sent: Sunday, March 15, 2009 10:48 AM To: jfish-at-gladstone.ucsf.edu
I'm not certain of the official guidelines for symptoms, but they may be similar to asthma, as it's also a condition of low O2 when flared.
__ blacking out, or vision seeming to de-pixil some.
__ Closing one's eyes after seeing a contrasted object, and still retaining the image for longer than normal when the eyes are closed.
__Slurred speech & slowed thinking
__ sometimes some dyslexia ( when I start mixing words it's time for the inhaler)
__ sometimes even stumbling on your own feet.
__fatigue, that goes away when you are not near the trigger
__ Nausea, vertigo
Lou Ann
bigelow-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } There have been a lot of comments on the dangers of hypoxia from } leaking or defective liquid nitrogen tanks. All very interesting. } } QUESTION: Are there any straightforward symptoms of the onset of } hypoxia that can be recognized in time to avoid succumbing to the } situation?? }
==============================Original Headers============================== 16, 21 -- From lamiller-at-illinois.edu Sun Mar 15 12:41:43 2009 16, 21 -- Received: from QMTA04.emeryville.ca.mail.comcast.net (qmta04.emeryville.ca.mail.comcast.net [76.96.30.40]) 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FHfgCP007104 16, 21 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:41:42 -0500 16, 21 -- Received: from OMTA04.emeryville.ca.mail.comcast.net ([76.96.30.35]) 16, 21 -- by QMTA04.emeryville.ca.mail.comcast.net with comcast 16, 21 -- id TTmD1b0070lTkoCA4Vhjvq; Sun, 15 Mar 2009 17:41:43 +0000 16, 21 -- Received: from lou-anns-computer.local ([98.212.148.206]) 16, 21 -- by OMTA04.emeryville.ca.mail.comcast.net with comcast 16, 21 -- id TVhh1b0044TRewa8QVhhUW; Sun, 15 Mar 2009 17:41:42 +0000 16, 21 -- Message-ID: {49BD3DD3.9050402-at-illinois.edu} 16, 21 -- Date: Sun, 15 Mar 2009 12:41:39 -0500 16, 21 -- From: Lou Ann Miller {lamiller-at-illinois.edu} 16, 21 -- User-Agent: Thunderbird 2.0.0.19 (Macintosh/20081209) 16, 21 -- MIME-Version: 1.0 16, 21 -- To: bigelow-at-umich.edu, microscopy-at-microscopy.com 16, 21 -- Subject: Re: [Microscopy] RE: symptoms of hypoxia 16, 21 -- References: {200903151729.n2FHTKUa004625-at-ns.microscopy.com} 16, 21 -- In-Reply-To: {200903151729.n2FHTKUa004625-at-ns.microscopy.com} 16, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 27 -- From jfish-at-gladstone.ucsf.edu Mon Mar 16 11:13:50 2009 25, 27 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GGDmxB007010 25, 27 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 11:13:50 -0500 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,373,1233561600"; 25, 27 -- d="scan'208";a="1770163" 25, 27 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25]) 25, 27 -- by gmail2.ucsf.edu with ESMTP; 16 Mar 2009 09:13:46 -0700 25, 27 -- Received: from [169.230.76.4] (HELO JFISH) 25, 27 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 4.2.10) 25, 27 -- with ESMTP id 566887528; Mon, 16 Mar 2009 09:13:46 -0700 25, 27 -- Reply-To: {jfish-at-gladstone.ucsf.edu} 25, 27 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu} 25, 27 -- To: {lamiller-at-illinois.edu} , {microscopy-at-microscopy.com} 25, 27 -- References: {200903151748.n2FHm0rX016075-at-ns.microscopy.com} 25, 27 -- Subject: RE: [Microscopy] Re: RE: symptoms of hypoxia 25, 27 -- Date: Mon, 16 Mar 2009 09:13:39 -0700 25, 27 -- Organization: J. David Gladstone Institutes 25, 27 -- Message-ID: {3E8F1A1B1054440BAE475CFF14918C6B-at-JFISH} 25, 27 -- MIME-Version: 1.0 25, 27 -- Content-Type: text/plain; 25, 27 -- charset="US-ASCII" 25, 27 -- Content-Transfer-Encoding: 7bit 25, 27 -- X-Mailer: Microsoft Office Outlook 11 25, 27 -- Thread-Index: AcmlljIKCsv6Hb5cRoKxD2/rudNAewAu9iQg 25, 27 -- In-Reply-To: {200903151748.n2FHm0rX016075-at-ns.microscopy.com} 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
All, I have been wondering if anyone has done a thorough comparison between a digital microscope, (Hirox, Keyence) and a conventional light microscope Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is one type more suited for given application(s)? What about cost? Portability?
There wasn't much information in the archives.
Paul J. Gerroir
Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
Informally, we've had demonstrations here of several types of microscopes, including conventional microscopes (Nikon) and digital microscopes (Keyence).
The three biggest differences we found were 1) Ease of capturing crisp, focused images at high to very high resolutions 2) the ability of the digital microscopes to generate images with substantial depth of field, due to on-the-fly processing of image stacks, and 3) the ability of the digital microscope to take an image at several angles, so that the software can produce a 3-d profile/image of the object. If you don't need the 3-d profiles, and can wait for image stacks to process, a conventional light microscope with a decent camera, software and motorized stage/focus would be a less expensive (but somewhat fuzzier) alternative.
Compared to the Keyence system, you are also giving up some portability; I don't know how much that would mean to you.
I have no connection to any of these companies.
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
-----Original Message----- X-from: paul.gerroir-at-xrcc.xeroxlabs.com [mailto:paul.gerroir-at-xrcc.xeroxlabs.com] Sent: Monday, March 16, 2009 11:26 AM To: Zonis, Robert
All, I have been wondering if anyone has done a thorough comparison between a digital microscope, (Hirox, Keyence) and a conventional light microscope Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is one type more suited for given application(s)? What about cost? Portability?
There wasn't much information in the archives.
Paul J. Gerroir
Microscopy Materials Characterization Xerox Research Centre of Canada 2660 Speakman Drive Mississauga, Ontario L5K 2L1
==============================Original Headers============================== 8, 32 -- From paul.gerroir-at-xrcc.xeroxlabs.com Mon Mar 16 11:15:59 2009 8, 32 -- Received: from wbmler4.mail.xerox.com (wbmler4.mail.xerox.com [13.13.138.219]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GGFvi9008192 8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 11:15:58 -0500 8, 32 -- Received: from wbmlir1.mail.xerox.com (wbmlir1.mail.xerox.com [13.131.8.221]) 8, 32 -- by wbmler4.mail.xerox.com (8.14.2/8.13.8) with ESMTP id n2GGFtsn016449 8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 12:15:55 -0400 8, 32 -- Received: from wbmlir1.mail.xerox.com (localhost [127.0.0.1]) 8, 32 -- by wbmlir1.mail.xerox.com (8.14.2/8.13.6) with ESMTP id n2GGFbFa009789 8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 12:15:37 -0400 8, 32 -- Received: from xrcc-sv8.xrcc.xeroxlabs.com ([13.2.46.182]) 8, 32 -- by wbmlir1.mail.xerox.com (8.14.2/8.13.6) with ESMTP id n2GGFbPn009778 8, 32 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 12:15:37 -0400 8, 32 -- X-XeroxINT-Source-Ip: 13.2.46.182 8, 32 -- X-XeroxINT-Source-Name: [13.2.46.182] 8, 32 -- X-XeroxINT-Reported-Name: xrcc-sv8.xrcc.xeroxlabs.com 8, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 32 -- Content-class: urn:content-classes:message 8, 32 -- MIME-Version: 1.0 8, 32 -- Content-Type: text/plain; 8, 32 -- charset="us-ascii" 8, 32 -- Subject: Light Microscopy 8, 32 -- Date: Mon, 16 Mar 2009 12:15:34 -0400 8, 32 -- Message-ID: {B4FD2284716D6C4DB94239C248E7CE00984D04-at-xrcc-sv8.xrcc.xeroxlabs.com} 8, 32 -- X-MS-Has-Attach: 8, 32 -- X-MS-TNEF-Correlator: 8, 32 -- Thread-Topic: Light Microscopy 8, 32 -- Thread-Index: AcmmUm8HVZNQtYx1QtCN0B8khQD+MA== 8, 32 -- From: "Gerroir, Paul" {paul.gerroir-at-xrcc.xeroxlabs.com} 8, 32 -- To: {Microscopy-at-Microscopy.com} 8, 32 -- Content-Transfer-Encoding: 8bit 8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GGFvi9008192 ==============================End of - Headers==============================
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==============================Original Headers============================== 24, 34 -- From Robert.Zonis-at-Sanford.com Mon Mar 16 11:56:13 2009 24, 34 -- Received: from mail96.messagelabs.com (mail96.messagelabs.com [216.82.254.19]) 24, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2GGuCoZ003515 24, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 11:56:12 -0500 24, 34 -- X-VirusChecked: Checked 24, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 24, 34 -- X-Msg-Ref: server-9.tower-96.messagelabs.com!1237222568!52470382!1 24, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 24, 34 -- X-Originating-IP: [198.176.16.25] 24, 34 -- Received: (qmail 6375 invoked from network); 16 Mar 2009 16:56:11 -0000 24, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25) 24, 34 -- by server-9.tower-96.messagelabs.com with SMTP; 16 Mar 2009 16:56:11 -0000 24, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 24, 34 -- Mon, 16 Mar 2009 11:56:06 -0500 24, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 34 -- Content-class: urn:content-classes:message 24, 34 -- MIME-Version: 1.0 24, 34 -- Content-Type: text/plain; 24, 34 -- charset="US-ASCII" 24, 34 -- Subject: RE: [Microscopy] Light Microscopy 24, 34 -- Date: Mon, 16 Mar 2009 11:54:17 -0500 24, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9402088517-at-naoaksasebe02.nr.ad.newellco.com} 24, 34 -- In-Reply-To: {200903161626.n2GGQHj4025322-at-ns.microscopy.com} 24, 34 -- X-MS-Has-Attach: 24, 34 -- X-MS-TNEF-Correlator: 24, 34 -- Thread-Topic: [Microscopy] Light Microscopy 24, 34 -- Thread-Index: AcmmU/BRt9VC/ZhITeagukCJyhvAQwAAS0Fw 24, 34 -- References: {200903161626.n2GGQHj4025322-at-ns.microscopy.com} 24, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 24, 34 -- To: {paul.gerroir-at-xrcc.xeroxlabs.com} 24, 34 -- Cc: {Microscopy-at-microscopy.com} 24, 34 -- X-OriginalArrivalTime: 16 Mar 2009 16:56:06.0260 (UTC) FILETIME=[18F1C340:01C9A658] 24, 34 -- Content-Transfer-Encoding: 8bit 24, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GGuCoZ003515 ==============================End of - Headers==============================
Hereby I inform you that due to the request of many colleagues:
The deadline of the CIASEM 2009 Pre-registration and Submission of Short Abstracts has been extended to APRIL 15, 2009.
We encourage you to participate in this important event to promote the collaboration towards the scientific and technological development of our Nations. Please visit our web page www.ciasem2009.com.ar
On behalf of the organizing Committee CIASEM 2009
----------------------------------------------- Dra. Patricia B.Bozzano Comisión Nacional de Energía Atómica Buenos Aires, Argentina Tel 54 11 6772 7395 / 7282 Fax 54 11 6772 7740
==============================Original Headers============================== 5, 27 -- From pbozzano-at-cnea.gov.ar Mon Mar 16 13:09:56 2009 5, 27 -- Received: from cnea.gov.ar (diavolo.cnea.gov.ar [200.68.249.35]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GI9trO020361 5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 13:09:56 -0500 5, 27 -- Received: from cnea-mail.cnea.gov.ar (cnea-mail.cnea.gov.ar [168.96.68.244]) 5, 27 -- by cnea.gov.ar (8.11.2/8.11.2) with ESMTP id n2GIBm528525 5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 15:11:48 -0300 5, 27 -- Received: from uam61 (uam061.cnea.gov.ar [192.168.16.62]) 5, 27 -- by cnea-mail.cnea.gov.ar (8.12.10/8.12.10) with ESMTP id n2GIBU5w016807 5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 15:11:30 -0300 5, 27 -- From: "Patricia B.Bozzano" {pbozzano-at-cnea.gov.ar} 5, 27 -- To: {Microscopy-at-microscopy.com} 5, 27 -- Subject: CIASEM 2009 5, 27 -- Date: Mon, 16 Mar 2009 15:09:01 -0300 5, 27 -- Organization: C.N.E.A. 5, 27 -- Message-ID: {001601c9a662$490993a0$db1cbae0$-at-gov.ar} 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; 5, 27 -- charset="iso-8859-1" 5, 27 -- X-Mailer: Microsoft Office Outlook 12.0 5, 27 -- Thread-Index: AcmmYkd7dyXexf8PQmCe4nPgo0bVEw== 5, 27 -- Content-Language: es-ar 5, 27 -- x-cr-hashedpuzzle: ByUf CJTr DPee ET29 Ef6b FL6x G4F1 G5Tq HIvt HZZz HgoT HhFU Hw4X IC8/ IGME JKyz;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{2A4D9FAB-A412-4EAE-AC73-F9939C0649B5};cABiAG8AegB6AGEAbgBvAEAAYwBuAGUAYQAuAGcAbwB2AC4AYQByAA==;Mon, 16 Mar 2009 18:08:59 GMT;QwBJAEEAUwBFAE0AIAAyADAAMAA5AA== 5, 27 -- x-cr-puzzleid: {2A4D9FAB-A412-4EAE-AC73-F9939C0649B5} 5, 27 -- X-RAVMilter-Version: 8.4.4(snapshot 20030410) (cnea-mail) 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GI9trO020361 ==============================End of - Headers==============================
Just out of curiosity, does anybody know what the price point on the JEOL Neoscope tabletop SEM is? I know the Hitachi and FEI models pricing, but I'm curious about this one. Also, has anybody used it and compared it to the other two? I'm fairly familiar with both Hitachi and FEI, but I haven't had a chance to test drive the JEOL yet.
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 3, 31 -- From kraftpiano-at-gmail.com Mon Mar 16 14:20:32 2009 3, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.147]) 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GJKWdt004414 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:20:32 -0500 3, 31 -- Received: by qw-out-1920.google.com with SMTP id 14so1974878qwa.54 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 12:20:32 -0700 (PDT) 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 31 -- d=gmail.com; s=gamma; 3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject 3, 31 -- :from:to:content-type:content-transfer-encoding; 3, 31 -- bh=NYqkjWTA/1cjInP32lUIU4VN+E2li56xQSyIcf5CyLw=; 3, 31 -- b=Q1ZFVjwsXvwn33Bol9MVLt4IyTg+nNKvP8f573yg1+PYYGNL88iRD36sZqXNrFIf5l 3, 31 -- v0lg7o8SVrDr8KHd9A/DKQcVuoFqFVXHdkYkZTEtLyrunabvKFKhnx9Cs7k9/rHGgQxV 3, 31 -- W/4EIFTp+fIdCUd0ZzyxVbHYfQGTJoWjSrMfs= 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 31 -- d=gmail.com; s=gamma; 3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type 3, 31 -- :content-transfer-encoding; 3, 31 -- b=iGa1lWGdBL6ljP9sZqf/V9YR17/277KkXAh6U4Mo6xn6fNPpiuITflrOSZL8U4oORQ 3, 31 -- IMpA62TF8aFN4XIxeITqIo8dNWr+JktOmCcGVXGxalcggbQXh5LQUr05+78V8zBXJ66v 3, 31 -- v88xnxVGMSADweZsUpgzuzykHFEIaARCiBXKI= 3, 31 -- MIME-Version: 1.0 3, 31 -- Received: by 10.224.54.76 with SMTP id p12mr6271346qag.207.1237231232379; Mon, 3, 31 -- 16 Mar 2009 12:20:32 -0700 (PDT) 3, 31 -- Date: Mon, 16 Mar 2009 15:20:32 -0400 3, 31 -- Message-ID: {25e2b0d20903161220p161de458n3915cf6786f2572b-at-mail.gmail.com} 3, 31 -- Subject: JEOL Tabletop SEM 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 31 -- To: microscopy-at-microscopy.com 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1 3, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I recently received a quote on the Nikon/JEOL for $60k. When I looked at all three models around a year ago, it seemed to me that the Nikon/ JEOL had the best compromise of mag, chamber size and cost, but things may have changed or I could have been mistaken. I have been following the JEOL, but not the other two.
Any updates on the other two would be appreciated. Also, I can forward along a quote and/or contact info offline if needed.
Thanks,
Gerhard
On Mar 16, 2009, at 15:22 , kraftpiano-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Just out of curiosity, does anybody know what the price point on the } JEOL Neoscope tabletop SEM is? I know the Hitachi and FEI models } pricing, but I'm curious about this one. Also, has anybody used it } and compared it to the other two? I'm fairly familiar with both } Hitachi and FEI, but I haven't had a chance to test drive the JEOL } yet. } } --Justin A. Kraft } } -- } "America believes in education; the average professor earns more money } in a year than a professional athlete earns in a whole week." Evan } Esar
==============================Original Headers============================== 8, 20 -- From schoenthal-at-vadiodes.com Mon Mar 16 14:28:25 2009 8, 20 -- Received: from mail312.opentransfer.com (mail312.opentransfer.com [98.130.1.170]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2GJSPMO017165 8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:28:25 -0500 8, 20 -- Received: (qmail 1604 invoked by uid 399); 16 Mar 2009 19:28:24 -0000 8, 20 -- Received: from unknown (HELO ?10.1.10.235?) (75.150.52.245) 8, 20 -- by mail312.opentransfer.com with ESMTP; 16 Mar 2009 19:28:24 -0000 8, 20 -- X-Originating-IP: 75.150.52.245 8, 20 -- From: "Gerhard S. Schoenthal" {schoenthal-at-vadiodes.com} 8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 20 -- In-Reply-To: {200903161922.n2GJMbWa007342-at-ns.microscopy.com} 8, 20 -- Subject: Re: [Microscopy] JEOL Tabletop SEM 8, 20 -- References: {200903161922.n2GJMbWa007342-at-ns.microscopy.com} 8, 20 -- Message-Id: {52869867-18AD-489B-B267-5047A784A697-at-vadiodes.com} 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- Mime-Version: 1.0 (Apple Message framework v930.3) 8, 20 -- Date: Mon, 16 Mar 2009 15:28:24 -0400 8, 20 -- Cc: kraftpiano-at-gmail.com 8, 20 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, March 16, 2009 12:29 PM To: dkloos-at-parallaxray.com
Just out of curiosity, does anybody know what the price point on the JEOL Neoscope tabletop SEM is? I know the Hitachi and FEI models pricing, but I'm curious about this one. Also, has anybody used it and compared it to the other two? I'm fairly familiar with both Hitachi and FEI, but I haven't had a chance to test drive the JEOL yet.
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 3, 31 -- From kraftpiano-at-gmail.com Mon Mar 16 14:20:32 2009 3, 31 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.147]) 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GJKWdt004414 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:20:32 -0500 3, 31 -- Received: by qw-out-1920.google.com with SMTP id 14so1974878qwa.54 3, 31 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 12:20:32 -0700 (PDT) 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 31 -- d=gmail.com; s=gamma; 3, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject 3, 31 -- :from:to:content-type:content-transfer-encoding; 3, 31 -- bh=NYqkjWTA/1cjInP32lUIU4VN+E2li56xQSyIcf5CyLw=; 3, 31 -- b=Q1ZFVjwsXvwn33Bol9MVLt4IyTg+nNKvP8f573yg1+PYYGNL88iRD36sZqXNrFIf5l 3, 31 -- v0lg7o8SVrDr8KHd9A/DKQcVuoFqFVXHdkYkZTEtLyrunabvKFKhnx9Cs7k9/rHGgQxV 3, 31 -- W/4EIFTp+fIdCUd0ZzyxVbHYfQGTJoWjSrMfs= 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 31 -- d=gmail.com; s=gamma; 3, 31 -- h=mime-version:date:message-id:subject:from:to:content-type 3, 31 -- :content-transfer-encoding; 3, 31 -- b=iGa1lWGdBL6ljP9sZqf/V9YR17/277KkXAh6U4Mo6xn6fNPpiuITflrOSZL8U4oORQ 3, 31 -- IMpA62TF8aFN4XIxeITqIo8dNWr+JktOmCcGVXGxalcggbQXh5LQUr05+78V8zBXJ66v 3, 31 -- v88xnxVGMSADweZsUpgzuzykHFEIaARCiBXKI= 3, 31 -- MIME-Version: 1.0 3, 31 -- Received: by 10.224.54.76 with SMTP id p12mr6271346qag.207.1237231232379; Mon, 3, 31 -- 16 Mar 2009 12:20:32 -0700 (PDT) 3, 31 -- Date: Mon, 16 Mar 2009 15:20:32 -0400 3, 31 -- Message-ID: {25e2b0d20903161220p161de458n3915cf6786f2572b-at-mail.gmail.com} 3, 31 -- Subject: JEOL Tabletop SEM 3, 31 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 31 -- To: microscopy-at-microscopy.com 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1 3, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 12, 30 -- From dkloos-at-parallaxray.com Mon Mar 16 14:55:29 2009 12, 30 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GJtSch000758 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 14:55:28 -0500 12, 30 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) 12, 30 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) 12, 30 -- (envelope-from {dkloos-at-parallaxray.com} ) 12, 30 -- id 1LjIuM-000ACo-PV; Mon, 16 Mar 2009 13:55:34 -0600 12, 30 -- Reply-To: {dkloos-at-parallaxray.com} 12, 30 -- From: "Don Kloos" {dkloos-at-parallaxray.com} 12, 30 -- To: {kraftpiano-at-gmail.com} 12, 30 -- Cc: {microscopy-at-microscopy.com} 12, 30 -- References: {200903161928.n2GJSdCC017785-at-ns.microscopy.com} 12, 30 -- Subject: RE: [Microscopy] JEOL Tabletop SEM 12, 30 -- Date: Mon, 16 Mar 2009 12:55:22 -0700 12, 30 -- Organization: Parallax Research 12, 30 -- Message-ID: {AC5B2B67C8F24A0192AB7AE2C11F6BDB-at-donl} 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; 12, 30 -- charset="us-ascii" 12, 30 -- Content-Transfer-Encoding: 7bit 12, 30 -- X-Mailer: Microsoft Office Outlook 11 12, 30 -- In-Reply-To: {200903161928.n2GJSdCC017785-at-ns.microscopy.com} 12, 30 -- Thread-Index: AcmmbW3EI6sIUVsOQwiQ8pKdz9MYqQAA6MHg 12, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 12, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 12, 30 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com 12, 30 -- X-AntiAbuse: Original Domain - microscopy.com 12, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] 12, 30 -- X-AntiAbuse: Sender Address Domain - parallaxray.com ==============================End of - Headers==============================
The symptoms vary with the degree of oxygen reduction. Also, some acclimatization can take place. Everest has been climbed by a few people without oxygen assist, but most people suddenly over 20,000 feet will have many symptoms similar to being drunk, hence the origin of the phrase "Getting High". The real danger is suddenly being in a situation where sufficient oxygen has been depleted from the air to cause a lethal situation. The fatality at Intel was caused when a technician started fill of an LS 160, venting the gas into an enclosed area with no automatic shut-off. He went to lunch, forgot about the dewar, and several hours later as he re-entered the room alone, the spring-loaded door closed behind him, sealing his doom. According to our safety people, one deep breath of 100% nitrogen can cause you to pass out. Similar situations occur tragically in workers repairing underground gas pipelines. Leaking gas can displace 100% of the air, and workers lose consciousness suddenly. Unfortunately, their would-be rescuers frequently suffer the same consequence, resulting in multiple fatalities. Another situation that the safety people were seriously concerned about was our darkroom. It had multiple nitrogen hoses with spring-loaded valves for dusting negatives prior to printing. Any failure overnight or over a weekend of a hose or valve could result in complete displacement of oxygen, and a technician entering the darkroom through the rotating doors could be in a lethal situation.
John Mardinly, Numonyx
-----Original Message----- X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu] Sent: Sunday, March 15, 2009 10:29 AM To: MARDINLY, A
There have been a lot of comments on the dangers of hypoxia from leaking or defective liquid nitrogen tanks. All very interesting.
QUESTION: Are there any straightforward symptoms of the onset of hypoxia that can be recognized in time to avoid succumbing to the situation?? -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
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==============================Original Headers============================== 11, 29 -- From A.MARDINLY-at-numonyx.com Mon Mar 16 17:08:42 2009 11, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GM8gCo019245 11, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 17:08:42 -0500 11, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23]) 11, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 869B4144024; 11, 29 -- Mon, 16 Mar 2009 16:10:39 -0400 (EDT) 11, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 11, 29 -- Mon, 16 Mar 2009 18:08:41 -0400 11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 29 -- Content-class: urn:content-classes:message 11, 29 -- MIME-Version: 1.0 11, 29 -- Content-Type: text/plain; 11, 29 -- charset="us-ascii" 11, 29 -- Subject: RE: [Microscopy] RE: symptoms of hypoxia 11, 29 -- Date: Mon, 16 Mar 2009 18:08:40 -0400 11, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F3A150-at-EXDRESBENMX012.numonyx.local} 11, 29 -- In-Reply-To: {200903151729.n2FHTGKm004498-at-ns.microscopy.com} 11, 29 -- X-MS-Has-Attach: 11, 29 -- X-MS-TNEF-Correlator: 11, 29 -- Thread-Topic: [Microscopy] RE: symptoms of hypoxia 11, 29 -- Thread-Index: Acmlk5Wyezl+sLUsTruME5wgVFj/NwA7dxlQ 11, 29 -- References: {200903151729.n2FHTGKm004498-at-ns.microscopy.com} 11, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 11, 29 -- To: {bigelow-at-umich.edu} 11, 29 -- Cc: {Microscopy-at-Microscopy.com} 11, 29 -- X-OriginalArrivalTime: 16 Mar 2009 22:08:41.0044 (UTC) FILETIME=[C3AAB140:01C9A683] 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GM8gCo019245 ==============================End of - Headers==============================
I generally agree with Robert Zonis's comments. The issue of portability is a major advantage of the Keyence and Hirox systems. The extended depth of field functions on this instruments is also very good.
However, in my opinion, some of the newer, more traditional light microscopes have superior image sharpness. We chose the Nikon AZ100 in favor of the Keyence, partially for the ease of use and improved sharpness for traditional bench microscopy. The Nikon extended depth of field software is not so good - I would stick with the CZM or similar freeware if this function is needed. The cost of the Nikon was also a factor - about 1/3 that of the Keyence system with similar magnification range. In the end, the best choice here will depend on how you will be using the instrument. I am very happy with the Nikon, but would love to have the Keyence or Hirox for some applications if I had the financial resources.
Good luck.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
} Paul, } } Informally, we've had demonstrations here of several types of } microscopes, including conventional microscopes (Nikon) and digital } microscopes (Keyence). } } The three biggest differences we found were 1) Ease of capturing crisp, } focused images at high to very high resolutions 2) the ability of the } digital microscopes to generate images with substantial depth of field, } due to on-the-fly processing of image stacks, and 3) the ability of the } digital microscope to take an image at several angles, so that the } software can produce a 3-d profile/image of the object. If you don't } need the 3-d profiles, and can wait for image stacks to process, a } conventional light microscope with a decent camera, software and } motorized stage/focus would be a less expensive (but somewhat fuzzier) } alternative. } } Compared to the Keyence system, you are also giving up some portability; } I don't know how much that would mean to you. } } I have no connection to any of these companies. } } Robert Zonis } Technical Service, LMTC } Sanford L.P. - A Newell Rubbermaid Company } Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } } This message is intended for the Microscopy Listserv. Permission is } specifically granted to the Microscopy Society of America to publish } some or all of this message in the Microscopy Today journal. } } } -----Original Message----- } X-from: paul.gerroir-at-xrcc.xeroxlabs.com } [mailto:paul.gerroir-at-xrcc.xeroxlabs.com] } Sent: Monday, March 16, 2009 11:26 AM } To: Zonis, Robert } Subject: [Microscopy] Light Microscopy } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } All, } I have been wondering if anyone has done a thorough comparison between a } digital microscope, (Hirox, Keyence) and a conventional light microscope } Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is } one type more suited for given application(s)? What about cost? } Portability? } } There wasn't much information in the archives. } } Paul J. Gerroir } } Microscopy } Materials Characterization } Xerox Research Centre of Canada } 2660 Speakman Drive } Mississauga, Ontario L5K 2L1 } } Phone: 905-823-7091, ext.216 } FAX: 905-822-7022 } e-mail: paul.gerroir-at-xerox.com
==============================Original Headers============================== 7, 25 -- From hanke-at-mee-inc.com Mon Mar 16 17:38:28 2009 7, 25 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GMcSqJ001209 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 17:38:28 -0500 7, 25 -- Received: (qmail 8381 invoked by uid 508); 16 Mar 2009 17:38:27 -0500 7, 25 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 7, 25 -- (perlscan: 1.24-st-qms. 7, 25 -- Clear:RC:1(216.14.180.82):. 7, 25 -- Processed in 0.098374 secs); 16 Mar 2009 22:38:27 -0000 7, 25 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 7, 25 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.098374 secs Process 8374) 7, 25 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 7, 25 -- by mail.namisolutions.com with SMTP; 16 Mar 2009 17:38:27 -0500 7, 25 -- Message-ID: {49BED4DD.2070401-at-mee-inc.com} 7, 25 -- Date: Mon, 16 Mar 2009 17:38:21 -0500 7, 25 -- From: Larry Hanke {hanke-at-mee-inc.com} 7, 25 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 7, 25 -- MIME-Version: 1.0 7, 25 -- To: microscopy-at-microscopy.com 7, 25 -- CC: paul.gerroir-at-xrcc.xeroxlabs.com 7, 25 -- Subject: RE: Light Microscopy 7, 25 -- References: {200903161656.n2GGuq4C004539-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {200903161656.n2GGuq4C004539-at-ns.microscopy.com} 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arnec-at-bio.umass.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arnec-at-bio.umass.edu Name: Arne Christensen
Organization: USGS - Conte Anadromous Fish Research Laboratory
Title-Subject: [Filtered] Looking for JEOL5800LV parts.
Question: Dear Listeners,
Approximately one year ago, we acquired a used JEOL5800LV SEM. According to the JEOL associate who came to survey the instrument, the unit is missing some critical components; a variable pumping unit for the low vacuum mode, a stage, and a converter. The variable pumping unit is make/model specific, and no longer being manufactured. So, I am on the look out for a replacement.
I'm wondering if anybody is aware of a JEOL5800LV SEM that is not operational, and may be used for parts.
It depends on what you are working with. I was quite impressed with some aspects of a Keyence demonstration we had a couple years ago. But they have never been able to duplicate some of the cross-polarized views I can routinely get with an old traditional Nikon. (I work with paint coatings that are often a mixture of transparent and opaque layers).
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Paul:
I generally agree with Robert Zonis's comments. The issue of portability is a major advantage of the Keyence and Hirox systems. The extended depth of field functions on this instruments is also very good.
However, in my opinion, some of the newer, more traditional light microscopes have superior image sharpness. We chose the Nikon AZ100 in favor of the Keyence, partially for the ease of use and improved sharpness for traditional bench microscopy. The Nikon extended depth of field software is not so good - I would stick with the CZM or similar freeware if this function is needed. The cost of the Nikon was also a factor - about 1/3 that of the Keyence system with similar magnification range. In the end, the best choice here will depend on how you will be using the instrument. I am very happy with the Nikon, but would love to have the Keyence or Hirox for some applications if I had the financial resources.
Good luck.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
} Paul, } } Informally, we've had demonstrations here of several types of } microscopes, including conventional microscopes (Nikon) and digital } microscopes (Keyence). } } The three biggest differences we found were 1) Ease of capturing crisp, } focused images at high to very high resolutions 2) the ability of the } digital microscopes to generate images with substantial depth of field, } due to on-the-fly processing of image stacks, and 3) the ability of the } digital microscope to take an image at several angles, so that the } software can produce a 3-d profile/image of the object. If you don't } need the 3-d profiles, and can wait for image stacks to process, a } conventional light microscope with a decent camera, software and } motorized stage/focus would be a less expensive (but somewhat fuzzier) } alternative. } } Compared to the Keyence system, you are also giving up some portability; } I don't know how much that would mean to you. } } I have no connection to any of these companies. } } Robert Zonis } Technical Service, LMTC } Sanford L.P. - A Newell Rubbermaid Company } Shelbyville, TN 37160 } Direct: +1 (931) 685-6635 } } This message is intended for the Microscopy Listserv. Permission is } specifically granted to the Microscopy Society of America to publish } some or all of this message in the Microscopy Today journal. } } } -----Original Message----- } X-from: paul.gerroir-at-xrcc.xeroxlabs.com } [mailto:paul.gerroir-at-xrcc.xeroxlabs.com] } Sent: Monday, March 16, 2009 11:26 AM } To: Zonis, Robert } Subject: [Microscopy] Light Microscopy } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } All, } I have been wondering if anyone has done a thorough comparison between a } digital microscope, (Hirox, Keyence) and a conventional light microscope } Olympus, Zeiss, Nikon, etc.). What are the advantages/disadvantages? Is } one type more suited for given application(s)? What about cost? } Portability? } } There wasn't much information in the archives. } } Paul J. Gerroir } } Microscopy } Materials Characterization } Xerox Research Centre of Canada } 2660 Speakman Drive } Mississauga, Ontario L5K 2L1 } } Phone: 905-823-7091, ext.216 } FAX: 905-822-7022 } e-mail: paul.gerroir-at-xerox.com
**************Feeling the pinch at the grocery store? Make meals for Under $10. (http://food.aol.com/frugal-feasts?ncid=emlcntusfood00000002)
==============================Original Headers============================== 13, 17 -- From DennisH658-at-aol.com Mon Mar 16 20:32:46 2009 13, 17 -- Received: from imo-d05.mx.aol.com (imo-d05.mx.aol.com [205.188.157.37]) 13, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2H1WkPP032185 13, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 20:32:46 -0500 13, 17 -- Received: from DennisH658-at-aol.com 13, 17 -- by imo-d05.mx.aol.com (mail_out_v39.1.) id w.c66.4bfff3aa (29672) 13, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 21:32:41 -0400 (EDT) 13, 17 -- From: DennisH658-at-aol.com 13, 17 -- Message-ID: {c66.4bfff3aa.36f057bd-at-aol.com} 13, 17 -- Date: Mon, 16 Mar 2009 21:32:45 EDT 13, 17 -- Subject: RE: Light Microscopy 13, 17 -- To: Microscopy-at-microscopy.com 13, 17 -- MIME-Version: 1.0 13, 17 -- Content-Type: text/plain; charset="US-ASCII" 13, 17 -- Content-Transfer-Encoding: 7bit 13, 17 -- X-Mailer: AOL 9.5 sub 29 13, 17 -- X-Spam-Flag: NO ==============================End of - Headers==============================
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
==============================Original Headers============================== 14, 26 -- From ph2-at-sprynet.com Mon Mar 16 22:10:42 2009 14, 26 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 14, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2H3AgEj018271 14, 26 -- for {microscopy-at-microscopy.com} ; Mon, 16 Mar 2009 22:10:42 -0500 14, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 26 -- s=dk20050327; d=sprynet.com; 14, 26 -- b=tmJy3GKYIac7iqiOHtXYrKi2PHh+a87O2cQFn/BzDew+oMFYsOwiHGvUanseIfIj; 14, 26 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP; 14, 26 -- Received: from [99.50.135.158] (helo=user915fa8f284) 14, 26 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 14, 26 -- (envelope-from {ph2-at-sprynet.com} ) 14, 26 -- id 1LjPhR-0000FB-I1; Mon, 16 Mar 2009 23:10:41 -0400 14, 26 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 14, 26 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 14, 26 -- Subject: FYI - Call for Papers: Inter/Micro 2009 14, 26 -- Date: Mon, 16 Mar 2009 23:10:30 -0400 14, 26 -- MIME-Version: 1.0 14, 26 -- Content-Type: text/plain; 14, 26 -- charset="us-ascii" 14, 26 -- Content-Transfer-Encoding: 7bit 14, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 14, 26 -- Thread-Index: Acmmre1jyWSZlS9FTyC3BDZv9UvEtg== 14, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 14, 26 -- Message-ID: {E1LjPhR-0000FB-I1-at-elasmtp-mealy.atl.sa.earthlink.net} 14, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9bd2dc4292b5adcb291b26e9375db49d6350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 14, 26 -- X-Originating-IP: 99.50.135.158 ==============================End of - Headers==============================
Dear All Does anyone know where we can get hold op parts for Jeol 1200's (mk1/2) TEM. We have been infomed by Jeol that many of the the parts are obselete but I am sure they must be available out there some where. Regards John
==============================Original Headers============================== 1, 31 -- From john.mitchels-at-gmail.com Tue Mar 17 03:49:43 2009 1, 31 -- Received: from mail-bw0-f160.google.com (mail-bw0-f160.google.com [209.85.218.160]) 1, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2H8ng3T016880 1, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 03:49:42 -0500 1, 31 -- Received: by bwz4 with SMTP id 4so4016756bwz.18 1, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 01:49:41 -0700 (PDT) 1, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 31 -- d=gmail.com; s=gamma; 1, 31 -- h=domainkey-signature:mime-version:received:date:message-id:subject 1, 31 -- :from:to:content-type:content-transfer-encoding; 1, 31 -- bh=rYiO4l2vF7rkwJQ+AWKjBcT0tF6/8PVUWAvquHY7jIM=; 1, 31 -- b=IiqTpg9iswTadjjGHjAgqM1NREUg0gkOr+OYfiw7Ryf4+776TYl1JaHBwivoK9ZqGk 1, 31 -- VrxM4fsaM7jKLf147ZAOnzbUeV1zAyl5XEVMF2j7jlCBx3lgATmG8P0TYxyftujEOIyI 1, 31 -- zjQiaM/yMRHDnHbibcxdspoI928vKGv3OXKv4= 1, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 31 -- d=gmail.com; s=gamma; 1, 31 -- h=mime-version:date:message-id:subject:from:to:content-type 1, 31 -- :content-transfer-encoding; 1, 31 -- b=Krfmbk3Rs5FZo7eNYUTxmAuqRaq5553ObJIfUiaKBX3JuGrSGy96HsMJ6s1vKIWQxQ 1, 31 -- Nskgegb+444JcH1HLGWNpdgmbazSGlayWR8273y2T97N4/hpxhZ/Stvp3gfPUBgditN1 1, 31 -- h+1lThhVIb/+CXvxGjIO3GTWcM0LB96HlBmtA= 1, 31 -- MIME-Version: 1.0 1, 31 -- Received: by 10.204.115.139 with SMTP id i11mr1955182bkq.199.1237279781784; 1, 31 -- Tue, 17 Mar 2009 01:49:41 -0700 (PDT) 1, 31 -- Date: Tue, 17 Mar 2009 08:49:41 +0000 1, 31 -- Message-ID: {1b3cf7c30903170149g76841a99o84174bd3ea88042e-at-mail.gmail.com} 1, 31 -- Subject: Wanted: Parts for Jeol 1200 EX (MK1/2) 1, 31 -- From: John Mitchels {john.mitchels-at-gmail.com} 1, 31 -- To: Microscopy-at-microscopy.com 1, 31 -- Content-Type: text/plain; charset=ISO-8859-1 1, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Although I suppose O2 monitors in liquid N2 tank storage rooms aren't much use if your PhD student draws off some and carries a large dewar into a small lift and promptly spills it there.
Actually this thread is useful, I regularly go into the our tissue/cell cryo-storage area filled with many very large tanks of N2, and promptly put my head into the top [vented] well of the N2 tanks trying in vain to find my rack of samples amongst the many ice covered ones there. And I can't actually remember giving the O2 monitor reading on the wall more than a cursory glance recently [it's generally very boring and always says the same thing], I just head for the samples - I guess I'm vaguely assuming an alarm would ring anyway if the O2 concentration falls, and that the room ventilation rate is fine [has been every visit so far], and I often go in chatting with a colleague which can be distracting.....but from now on..
Actually, I have been victim to gas heater carbon monoxide poisoning while in my student dive [age 22], and no, other than feeling lousy [thought I had flu] I didn't notice the high CO gas levels at all [being odourless and all, just like N2]. My mates parents suffered similarly years earlier while watching TV, and only survived because the gas fire's pay-as-you-go gas meter ran out of money and switched off the gas fire - they woke up at 4.00 in the morning. I survived because my attempts at draft exclusion in my bedsit weren't entirely successful. Presumably CO poisoning is like a lack of O2 as it blocks the O2 receptors in haemoglobin [but unlike cyanide, its reversible if you happen to have some pure oxygen about].
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
} } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Folks - } } Personally, I would like this thread to } turn away from "hypoxia", the history, } or symptoms. } } The MSDS, fire code, etc.... clearly state } "do not store LN2 is confined spaces". Additionally, } there must be excellent ventilation. } } Some suggest to wear a self-contained breathing } apparatus if the oxygen content routinely falls } below 19%. } } In New York City, you cannot legally keep a dewar } within a few feet of a door or exit. Infact, it } is illegal there to store LN2 is a public hallway. } } Hence, let us be preventive. An oxygen monitor from } MSA (Mine Safety Appliances) can be bought from } Lab Safety Supply, Fisher Sci, etc... for just $200. } } regards, } } JQuinn } } } } ==============================Original } Headers============================== } 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sun Mar 15 13:59:21 2009 } 11, 12 -- Received: from www.matscieng.sunysb.edu } (www.matscieng.sunysb.edu [129.49.36.33]) } 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n2FIxLPu022978 } 11, 12 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 13:59:21 } -0500 } 11, 12 -- Received: (from jquinn-at-localhost) } 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id n2FJwrj16350 } 11, 12 -- for microscopy-at-microscopy.com; Sun, 15 Mar 2009 14:58:53 -0500 } 11, 12 -- Date: Sun, 15 Mar 2009 14:58:53 -0500 } 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} } 11, 12 -- Message-Id: {200903151958.n2FJwrj16350-at-www.matscieng.sunysb.edu} } 11, 12 -- To: microscopy-at-microscopy.com } 11, 12 -- Subject: re: LN2 in confined space } ==============================End of - } Headers============================== }
==============================Original Headers============================== 17, 20 -- From kjmorris-at-well.ox.ac.uk Tue Mar 17 05:35:21 2009 17, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HAZLZR001890 17, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 05:35:21 -0500 17, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 17, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 17, 20 -- id 1LjWdk-00013n-Jj 17, 20 -- for microscopy-at-microscopy.com; Tue, 17 Mar 2009 10:35:20 +0000 17, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 17, 20 -- To: {microscopy-at-microscopy.com} 17, 20 -- Subject: FW: [Microscopy] re: LN2 in confined space 17, 20 -- Date: Tue, 17 Mar 2009 10:35:20 -0000 17, 20 -- Message-ID: {4F3698AC996149FEB9602101FCA7ADDC-at-CytoWhizz} 17, 20 -- MIME-Version: 1.0 17, 20 -- Content-Type: text/plain; 17, 20 -- charset="us-ascii" 17, 20 -- Content-Transfer-Encoding: 7bit 17, 20 -- X-Mailer: Microsoft Office Outlook 11 17, 20 -- Thread-Index: AcmlzbMxrrQS/gZVSVeALuPG3QKlcwAU40yw 17, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 ==============================End of - Headers==============================
This looks like it might be a good topic for a paper.
Let me outline a few things:
Effects and symptoms (by % or ppm) of too low of O2 (easy table that I already have; also altitude data and partial pressures)
Effects and symptoms (by % or ppm) of too high of N2 (most data is on divers including nitrogen narcosis)
Effects and symptoms (by % or ppm) of too high CO2 (best data is on submarine occupants, good opportunity to discuses short term limits)
Effects and symptoms (by % or ppm) of too high of O2 (best data on rats at Navy Toxic Lab at Wright-Pat based on post Apollo failure testing but some human; I'll see if this is releasable)
Good opportunity to discuss air exchange rates, decay, and time to re-entry (I did some work on this for Dams that use CO2 cylinders to flood the facility when an electrical fire occurs, just have to find it)
I could also discuss certain refrigerants (HCFCs for instance) that have cardiac sensitization issues at high ppm (5-80,000 ppm [0.5-8%]) upon release.
Would y'all be interested in an article?
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: A.MARDINLY-at-numonyx.com [mailto:A.MARDINLY-at-numonyx.com] Sent: Monday, March 16, 2009 6:13 PM To: ph2-at-sprynet.com
The symptoms vary with the degree of oxygen reduction. Also, some acclimatization can take place. Everest has been climbed by a few people without oxygen assist, but most people suddenly over 20,000 feet will have many symptoms similar to being drunk, hence the origin of the phrase "Getting High". The real danger is suddenly being in a situation where sufficient oxygen has been depleted from the air to cause a lethal situation. The fatality at Intel was caused when a technician started fill of an LS 160, venting the gas into an enclosed area with no automatic shut-off. He went to lunch, forgot about the dewar, and several hours later as he re-entered the room alone, the spring-loaded door closed behind him, sealing his doom. According to our safety people, one deep breath of 100% nitrogen can cause you to pass out. Similar situations occur tragically in workers repairing underground gas pipelines. Leaking gas can displace 100% of the air, and workers lose consciousness suddenly. Unfortunately, their would-be rescuers frequently suffer the same consequence, resulting in multiple fatalities. Another situation that the safety people were seriously concerned about was our darkroom. It had multiple nitrogen hoses with spring-loaded valves for dusting negatives prior to printing. Any failure overnight or over a weekend of a hose or valve could result in complete displacement of oxygen, and a technician entering the darkroom through the rotating doors could be in a lethal situation.
John Mardinly, Numonyx
-----Original Message----- X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu] Sent: Sunday, March 15, 2009 10:29 AM To: MARDINLY, A
There have been a lot of comments on the dangers of hypoxia from leaking or defective liquid nitrogen tanks. All very interesting.
QUESTION: Are there any straightforward symptoms of the onset of hypoxia that can be recognized in time to avoid succumbing to the situation?? -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 14 -- From bigelow-at-umich.edu Sun Mar 15 12:12:54 2009 2, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 2, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2FHCqTn025128 2, 14 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 12:12:53 -0500 2, 14 -- Received: FROM [76.234.131.113] (adsl-76-234-131-113.dsl.sfldmi.sbcglobal.net [76.234.131.113]) 2, 14 -- BY hellskitchen.mr.itd.umich.edu ID 49BD3711.9A774.25996 ; 2, 14 -- 15 Mar 2009 13:12:50 -0400 2, 14 -- Mime-Version: 1.0 2, 14 -- Message-Id: {p06240800c5e2e6aed43a-at-[99.130.26.88]} 2, 14 -- Date: Sun, 15 Mar 2009 13:12:46 -0400 2, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 14 -- Subject: [Microscopy] RE: symptoms of hypoxia 2, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 11, 29 -- From A.MARDINLY-at-numonyx.com Mon Mar 16 17:08:42 2009 11, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2GM8gCo019245 11, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 16 Mar 2009 17:08:42 -0500 11, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23]) 11, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 869B4144024; 11, 29 -- Mon, 16 Mar 2009 16:10:39 -0400 (EDT) 11, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.39]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 11, 29 -- Mon, 16 Mar 2009 18:08:41 -0400 11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 29 -- Content-class: urn:content-classes:message 11, 29 -- MIME-Version: 1.0 11, 29 -- Content-Type: text/plain; 11, 29 -- charset="us-ascii" 11, 29 -- Subject: RE: [Microscopy] RE: symptoms of hypoxia 11, 29 -- Date: Mon, 16 Mar 2009 18:08:40 -0400 11, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9F3A150-at-EXDRESBENMX012.numonyx.local} 11, 29 -- In-Reply-To: {200903151729.n2FHTGKm004498-at-ns.microscopy.com} 11, 29 -- X-MS-Has-Attach: 11, 29 -- X-MS-TNEF-Correlator: 11, 29 -- Thread-Topic: [Microscopy] RE: symptoms of hypoxia 11, 29 -- Thread-Index: Acmlk5Wyezl+sLUsTruME5wgVFj/NwA7dxlQ 11, 29 -- References: {200903151729.n2FHTGKm004498-at-ns.microscopy.com} 11, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 11, 29 -- To: {bigelow-at-umich.edu} 11, 29 -- Cc: {Microscopy-at-Microscopy.com} 11, 29 -- X-OriginalArrivalTime: 16 Mar 2009 22:08:41.0044 (UTC) FILETIME=[C3AAB140:01C9A683] 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2GM8gCo019245 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From ph2-at-sprynet.com Tue Mar 17 08:06:50 2009 33, 28 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HD6oOZ019989 33, 28 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 08:06:50 -0500 33, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 33, 28 -- s=dk20050327; d=sprynet.com; 33, 28 -- b=UbnDQHab5DRAWGYmawEK/jb9z0WKxf0vPWp+kzXrVFDhdmXlSlJtt8LoPa8X29lz; 33, 28 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:In-Reply-To:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP; 33, 28 -- Received: from [75.61.18.94] (helo=user915fa8f284) 33, 28 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 33, 28 -- (envelope-from {ph2-at-sprynet.com} ) 33, 28 -- id 1LjZ0L-0003qc-7q; Tue, 17 Mar 2009 09:06:49 -0400 33, 28 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 33, 28 -- To: {A.MARDINLY-at-numonyx.com} , 33, 28 -- "Microscopy Listserve" {microscopy-at-microscopy.com} 33, 28 -- Subject: RE: [Microscopy] RE: RE: symptoms of hypoxia 33, 28 -- Date: Tue, 17 Mar 2009 09:06:38 -0400 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="us-ascii" 33, 28 -- Content-Transfer-Encoding: 7bit 33, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 33, 28 -- Thread-Index: AcmmhHLgbVpla2ELTC24hwS8QiW6ZwAfJQiw 33, 28 -- In-Reply-To: {200903162213.n2GMDMvA026464-at-ns.microscopy.com} 33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 33, 28 -- Message-ID: {E1LjZ0L-0003qc-7q-at-elasmtp-mealy.atl.sa.earthlink.net} 33, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f91331076f26a8a30b5fa6a0626caa7495350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 33, 28 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
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Question: I have a problem with a stereoscan 240 (Cambridge). When i turn it on, the vacuum led lights on (then the microscope is ready for the high voltage) and i press the operate button i had no raster on the screen. Also the beam led does not light on (neither the fail, or the trip). Does any one know what is the problem and how can i correct it?
Large dewars of liquid nitrogen should never ride an elevator with people in it. On the UC Berkeley campus and at the Lawrence Berkeley Lab large LN2 dewars ride the elevator alone with a large sign warning not to get on with it. The researcher takes a different elevator or runs the stairs.
Roseann
Roseann Csencsits, PhD Scientist in Charge - Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Mar 17, 2009, at 3:42 AM, kjmorris-at-well.ox.ac.uk wrote:
} } Although I suppose O2 monitors in liquid N2 tank storage rooms } aren't much } use if your PhD student draws off some and carries a large dewar } into a } small lift and promptly spills it there.
==============================Original Headers============================== 13, 26 -- From RCsencsits-at-lbl.gov Tue Mar 17 11:04:46 2009 13, 26 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HG4hCj021128 13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 11:04:45 -0500 13, 26 -- X-Ironport-SBRS: 2.3 13, 26 -- X-IronPort-Anti-Spam-Filtered: true 13, 26 -- X-IronPort-Anti-Spam-Result: AucCAFdmv0mAAykYe2dsb2JhbACVWQEBFiIFr2EJjzOCSYEzBmGGNg 13, 26 -- X-IronPort-AV: E=Sophos;i="4.38,379,1233561600"; 13, 26 -- d="scan'208";a="97188986" 13, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 13, 26 -- by ironport2.lbl.gov with ESMTP; 17 Mar 2009 09:04:42 -0700 13, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246]) 13, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n2HG4fhD001121; 13, 26 -- Tue, 17 Mar 2009 09:04:41 -0700 (PDT) 13, 26 -- Cc: Microscopy-at-microscopy.com 13, 26 -- Message-Id: {E16E186E-E62F-407B-BDDD-E512B8A60B8C-at-lbl.gov} 13, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 13, 26 -- To: kjmorris-at-well.ox.ac.uk 13, 26 -- In-Reply-To: {200903171042.n2HAgXDX012322-at-ns.microscopy.com} 13, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 26 -- Content-Transfer-Encoding: 7bit 13, 26 -- Mime-Version: 1.0 (Apple Message framework v930.3) 13, 26 -- Subject: Re: [Microscopy] FW: re: LN2 in confined space 13, 26 -- Date: Tue, 17 Mar 2009 07:20:19 -0700 13, 26 -- References: {200903171042.n2HAgXDX012322-at-ns.microscopy.com} 13, 26 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
On Mar 17, 2009, at 6:06 AM, ph2-at-sprynet.com wrote:
} Would y'all be interested in an article?
Dear Tony, I'd be interested. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Tue Mar 17 11:49:55 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HGnsfb003813 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 11:49:55 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 4E1BC2E50C72 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 09:49:53 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 60F902E50C6E 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 09:49:52 -0700 (PDT) 6, 22 -- Message-Id: {96948574-53EB-47BD-8DB8-BA4538E205BA-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200903171306.n2HD6w57020113-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] RE: symptoms of hypoxia 6, 22 -- Date: Tue, 17 Mar 2009 09:49:51 -0700 6, 22 -- References: {200903171306.n2HD6w57020113-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Of course people are often injured by not following the written safety procedures, hence my comment about an inexperienced young PhD student in a hurry. Safety interlocks bypassed, category IV lasers operated without any shielding or eye protection, burners fired up without a flame trap.. etc... and I know I haven't seen it all.
That said health & Safety is far superior these days compared to when I started my PhD in fuel and combustion back in the late 1970s [two people died in that department while I was there - one senior lecturer killed by carbon monoxide poisoning from a gas cylinder, the other a PhD student killed by a gas meter explosion [no flame trap fitted]. I carried on using that same carbon monoxide gas cylinder, with one modification - a jubilee clip was fitted, clamping the clear plastic tubing to the pressure regulator to 'prevent' it slipping off again.
Our Oxford Universities Health & Safety POLICY STATEMENT S4/03 hopefully says it all about using liquid nitrogen safely: http://www.admin.ox.ac.uk/safety/s403.shtml
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: Roseann Csencsits [mailto:RCsencsits-at-lbl.gov] Sent: 17 March 2009 14:20 To: kjmorris-at-well.ox.ac.uk Cc: Microscopy-at-Microscopy.Com
You can reverse cyanide poisoning as well, you just need amyl nitrate. This is routinely carried by all sensible possum trappers here in New Zealand to prevent accidental poisoning when in the wilds.
Presumably CO poisoning is like a lack of O2 as it blocks the O2 receptors in haemoglobin [but unlike cyanide, its reversible if you happen to have some pure oxygen about].
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
} } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Folks - } } Personally, I would like this thread to } turn away from "hypoxia", the history, } or symptoms. } } The MSDS, fire code, etc.... clearly state } "do not store LN2 is confined spaces". Additionally, } there must be excellent ventilation. } } Some suggest to wear a self-contained breathing } apparatus if the oxygen content routinely falls } below 19%. } } In New York City, you cannot legally keep a dewar } within a few feet of a door or exit. Infact, it } is illegal there to store LN2 is a public hallway. } } Hence, let us be preventive. An oxygen monitor from } MSA (Mine Safety Appliances) can be bought from } Lab Safety Supply, Fisher Sci, etc... for just $200. } } regards, } } JQuinn } } } } ==============================Original } Headers============================== } 11, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sun Mar 15 13:59:21 2009 } 11, 12 -- Received: from www.matscieng.sunysb.edu } (www.matscieng.sunysb.edu [129.49.36.33]) } 11, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n2FIxLPu022978 } 11, 12 -- for {microscopy-at-microscopy.com} ; Sun, 15 Mar 2009 13:59:21 } -0500 } 11, 12 -- Received: (from jquinn-at-localhost) } 11, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id n2FJwrj16350 } 11, 12 -- for microscopy-at-microscopy.com; Sun, 15 Mar 2009 14:58:53 -0500 } 11, 12 -- Date: Sun, 15 Mar 2009 14:58:53 -0500 } 11, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} } 11, 12 -- Message-Id: {200903151958.n2FJwrj16350-at-www.matscieng.sunysb.edu} } 11, 12 -- To: microscopy-at-microscopy.com } 11, 12 -- Subject: re: LN2 in confined space } ==============================End of - } Headers============================== }
==============================Original Headers============================== 17, 20 -- From kjmorris-at-well.ox.ac.uk Tue Mar 17 05:35:21 2009 17, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HAZLZR001890 17, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 05:35:21 -0500 17, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 17, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 17, 20 -- id 1LjWdk-00013n-Jj 17, 20 -- for microscopy-at-microscopy.com; Tue, 17 Mar 2009 10:35:20 +0000 17, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 17, 20 -- To: {microscopy-at-microscopy.com} 17, 20 -- Subject: FW: [Microscopy] re: LN2 in confined space 17, 20 -- Date: Tue, 17 Mar 2009 10:35:20 -0000 17, 20 -- Message-ID: {4F3698AC996149FEB9602101FCA7ADDC-at-CytoWhizz} 17, 20 -- MIME-Version: 1.0 17, 20 -- Content-Type: text/plain; 17, 20 -- charset="us-ascii" 17, 20 -- Content-Transfer-Encoding: 7bit 17, 20 -- X-Mailer: Microsoft Office Outlook 11 17, 20 -- Thread-Index: AcmlzbMxrrQS/gZVSVeALuPG3QKlcwAU40yw 17, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 31 -- From r.gilbert-at-auckland.ac.nz Tue Mar 17 15:41:03 2009 22, 31 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35]) 22, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HKeuXE006228 22, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Mar 2009 15:41:02 -0500 22, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 22, 31 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 65FCC482754 22, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:40:54 +1300 (NZDT) 22, 31 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 22, 31 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 22, 31 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 22, 31 -- with ESMTP id Jt+FVScm-WpK for {Microscopy-at-microscopy.com} ; 22, 31 -- Wed, 18 Mar 2009 09:40:54 +1300 (NZDT) 22, 31 -- Received: from fmhsx1.fmhs.auckland.ac.nz (fmhsx1.fmhs.auckland.ac.nz [130.216.128.61]) 22, 31 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1A39C4826C2 22, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:40:54 +1300 (NZDT) 22, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 31 -- Content-class: urn:content-classes:message 22, 31 -- MIME-Version: 1.0 22, 31 -- Content-Type: text/plain; 22, 31 -- charset="iso-8859-1" 22, 31 -- Subject: FW: [Microscopy] FW: re: LN2 in confined space 22, 31 -- Date: Wed, 18 Mar 2009 09:40:06 +1300 22, 31 -- Message-ID: {738FC0785373EC41B3B88A65CFBC981501C8B37C-at-FMHSX1.fmhs.auckland.ac.nz} 22, 31 -- X-MS-Has-Attach: 22, 31 -- X-MS-TNEF-Correlator: 22, 31 -- Thread-Topic: [Microscopy] FW: re: LN2 in confined space 22, 31 -- thread-index: Acmm7O8kWxGYjNweSiufdThtmXWC+QAUzLGg 22, 31 -- From: "Ray Gilbert" {r.gilbert-at-auckland.ac.nz} 22, 31 -- To: {Microscopy-at-microscopy.com} 22, 31 -- Content-Transfer-Encoding: 8bit 22, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2HKeuXE006228 ==============================End of - Headers==============================
This has just appeared in a Sigma Xi Email newsletter:
"PUBLISHER LOOKING FOR CHILDREN'S BOOKS ON SCIENCE Magic World Media is a new children's book publishing company focused on publishing imaginative picture books and early chapter books on scientific topics that extend the world view of children beyond their sensory experience and introduce them to the vastness of what is still unknown. Their first titles will be launched in the fall of this year. They are now actively seeking manuscripts for publication in 2010 and beyond on various topics and are particularly interested in the topics of dark matter and light. Visit http://www.magicworldmedia.com to view submission guidelines. To be considered for publication in 2010, picture book manuscripts must be received by May 31, 2009, and chapter book manuscripts by October 31." -- -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 2, 16 -- From schooley-at-mcn.org Tue Mar 17 16:12:32 2009 2, 16 -- Received: from dns3.mcn.org (dns3.mcn.org [216.150.240.32]) 2, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2HLCTsA020572 2, 16 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Mar 2009 16:12:30 -0500 2, 16 -- Received: from [66.81.65.112] (helo=[66.81.64.22]) 2, 16 -- by dns3.mcn.org with esmtpa (Exim 4.69) 2, 16 -- (envelope-from {schooley-at-mcn.org} ) 2, 16 -- id 1LjgaI-0008Nd-3d 2, 16 -- for Microscopy-at-Microscopy.Com; Tue, 17 Mar 2009 14:12:27 -0700 2, 16 -- Mime-Version: 1.0 2, 16 -- Message-Id: {a06200707c5e5c495b53c-at-[66.81.64.22]} 2, 16 -- Date: Tue, 17 Mar 2009 14:23:10 -0700 2, 16 -- To: Microscopy-at-Microscopy.Com 2, 16 -- From: Caroline Schooley {schooley-at-mcn.org} 2, 16 -- Subject: Microscopy education 2, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I know many of you are probably in the process of submitting equipment grant to acquire new instrument due to the stimulus package (I come in peace, my fellow competitors!). I wonder if any of you are willing to share your experience in using RMC cryo-ultramicrotome, particularly the pros and cons of the RMC cryo-ultramicrotome compared to Leica UC6 and FC6. I am also very interested to know any opinion about RMC freeze substitution machine (FS7500) and its comparison to Leica AFS2. I appreciate any feedback/comment if you have used any of these instruments. Thank you so much.
If you are concerned about conflict of interest, please rely to my personal e-mail.
It was isolated from macrophages from the peripheral blood of sarcoidosis patients. The same organism was also isolated from the macrophages of a pleural effusion from an HIV positve patient with diffuse large B cell lymphoma after many months of culture. It was fixed in glutaraldehyde in Sorensens buffer,pelleted, and embedded in Histogel. Then the Histogel-embedded pellet was prepared for TEM by standard techniques, i.e., post fixed with osmium, en bloc stained with uranyl acetate, dehydrated in a graded series of ethanol followed by propylene oxide, and infiltrated and embedded in Epon. Ultra-thin sections were post stained with uranyl acetate and lead citrate.
Has anyone seen anything like this? We would like to give it its proper name instead of its current name, The Critter. Any help you might give us would be appreciated.
Thanks,
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School A807 BSRB 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 11, 16 -- From dsoren-at-umich.edu Wed Mar 18 07:11:12 2009 11, 16 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 11, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2ICBCsP023154 11, 16 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 07:11:12 -0500 11, 16 -- Received: FROM [10.21.131.86] (host-18.subnet-17.med.umich.edu [141.214.17.18]) 11, 16 -- BY hackers.mr.itd.umich.edu ID 49C0E4A4.5EC54.30427 ; 11, 16 -- 18 Mar 2009 08:10:12 -0400 11, 16 -- Mime-Version: 1.0 (Apple Message framework v753.1) 11, 16 -- Content-Transfer-Encoding: 7bit 11, 16 -- Message-Id: {FD3BC54A-F5AC-43D2-BA3C-36B68D2378A8-at-umich.edu} 11, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 16 -- To: microscopy-at-microscopy.com 11, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 11, 16 -- Subject: What is this critter? 11, 16 -- Date: Wed, 18 Mar 2009 08:10:05 -0400 11, 16 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both giulio.lamedica-at-libero.it as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: giulio.lamedica-at-libero.it Name: Giulio
Organization: Univ of Rome La Sapienza
Title-Subject: [Filtered] TiO2 50 100nm nanoparticles in a liquid suspension
Question: I'm interested in analysing TiO2 50 100nm nanoparticles in a liquid suspension with FE-SEM. I'm intereted in particles characterization, because we know exactly their shape. What I'd like to investigate is how they are aggregate. Of course if we dry them we change their aggregation state. Have you any ideas to suggest?
We remind you that the 4th *Piezoresponse Force Microscopy* (PFM) Workshop and the first symposium on *Nanoscale Phenomena in Polar Materials* (PFM 2009) will take place in Aveiro (Portugal) 23-27 of June 2009.
If you intend to participate, we kindly remind you that the deadline for registration is approaching rapidly.
We offer a number of tutorial lectures by PFM specialists (see list with abstracts at http://pfm4.web.ua.pt, hands-on classes (optional) with advanced setups from *Agilent*, *Asylum*, *NT-MDT* and *Veeco* and participation in the symposium with the possibility to discuss your latest results in a warm and cordial atmosphere.
Many renowned scientists have already agreed to participate as plenary, invited, and tutorial speakers, including M. Alexe, R. Garcia, A. Gruverman, S. V. Kalinin, W. Kleemann, A. Ruediger, P. Paruch, N. Pertsev, G. Schneider, J. F. Scott, J.-M. Triscone, and others. Do not miss this opportunity to learn the latest developments in the rapidly growing world of *PFM* and *NanoFerroelectrics*!
With our warmest regards, we look forward to seeing you in June in Aveiro.
Sincerely
Sergei V. Kalinin
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 13, 24 -- From sergei2-at-ornl.gov Wed Mar 18 09:43:17 2009 13, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IEhGSS023023 13, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:43:17 -0500 13, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 13, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 13, 24 -- with ESMTP id {0KGP00IEZI823T-at-emroute1.ornl.gov} for 13, 24 -- microscopy-at-microscopy.com; Wed, 18 Mar 2009 10:43:14 -0400 (EDT) 13, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 13, 24 -- (PMDF V6.4 #31561) id {0KGP00001I82KI-at-emroute1.ornl.gov} ; Wed, 13, 24 -- 18 Mar 2009 10:43:14 -0400 (EDT) 13, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 13, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 13, 24 -- with ESMTP id {0KGP00JEFI8242-at-emroute1.ornl.gov} ; Wed, 13, 24 -- 18 Mar 2009 10:43:14 -0400 (EDT) 13, 24 -- Date: Wed, 18 Mar 2009 10:43:14 -0400 13, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 13, 24 -- Subject: PFM4 - Aveiro 13, 24 -- To: microscopy-at-microscopy.com, Andrei Kholkin {kholkin-at-ua.pt} 13, 24 -- Message-id: {49C10882.60501-at-ornl.gov} 13, 24 -- MIME-version: 1.0 13, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 13, 24 -- Content-transfer-encoding: 7bit 13, 24 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) ==============================End of - Headers==============================
It looks like a large icosahedral virus and is similar in size to Herpesvirus. There are not many large (} 100nm) icosahedral viruses that infect vertebrates but I'd still suggest you consider it. The third image has one very clear particle in the cytoplasm and near the top of the same image, a number of particles that look to be budding. -paul
-- Paul Chipman Director, Biological Electron Microscopy Facility Purdue University 765-494-1487
Quoting dsoren-at-umich.edu:
-------------------------------------------------------------- } } Dear Listers, } } I am writing to enlist your help in identifying the organism in these } TEM images, which } you can view by going to this link. } } http://www.med.umich.edu/cdb/mil/docs/temimages.html } } It was isolated from macrophages from the peripheral blood of } sarcoidosis patients. The same organism was also isolated from } the macrophages of a pleural effusion from an HIV positve patient } with diffuse large B cell lymphoma after many months of culture. It } was fixed in glutaraldehyde in Sorensens buffer,pelleted, and } embedded in Histogel. Then the Histogel-embedded pellet was } prepared for TEM by standard techniques, i.e., post fixed with osmium, } en bloc stained with uranyl acetate, dehydrated in a graded series of } ethanol followed by propylene oxide, and infiltrated and embedded in } Epon. Ultra-thin sections were post stained with uranyl acetate and lead } citrate. } } Has anyone seen anything like this? We would like to give it its } proper name instead of its current name, The Critter. Any help you } might give us would be appreciated. } } Thanks, } } Dotty Sorenson } } } Dorothy Sorenson } Microscopy and Image-analysis Laboratory } Department of Cell and Developmental Biology } University Of Michigan Medical School } A807 BSRB } 109 Zina Pitcher Place } Ann Arbor, MI 48109-2200 } (734)763-1170 } FAX (734)763-1166 }
==============================Original Headers============================== 7, 20 -- From paulrc-at-bilbo.bio.purdue.edu Wed Mar 18 10:27:42 2009 7, 20 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IFRe2O005496 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:27:40 -0500 7, 20 -- Received: from dhcp18-69-fixed.bio.purdue.edu (dhcp18-69-fixed.bio.purdue.edu [128.210.18.69]) 7, 20 -- (authenticated bits=0) 7, 20 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-auth.purdue.edu) with ESMTP id n2IFRcIC000907 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:27:38 -0400 7, 20 -- Message-ID: {49C112EA.6060703-at-bilbo.bio.purdue.edu} 7, 20 -- Date: Wed, 18 Mar 2009 11:27:38 -0400 7, 20 -- From: "Chipman, Paul R" {paulrc-at-bilbo.bio.purdue.edu} 7, 20 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 7, 20 -- MIME-Version: 1.0 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- Subject: Re: [Microscopy] What is this critter? 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-PMX-Version: 5.4.0.320885 7, 20 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
If you don't want to dry the particles you could deposit a small volume onto a TEM grid and plunge freeze the grid in liquid ethane at liq. N2 temps and looks at it in a cryo-TEM. You didn't mention what medium the nanoparticles were dispersed in but if its water that should work. Some sort of x-ray or light (laser) scattering technique might be able to identify the aggregate size without needing to dry the suspension.
AFM in solution could work but I don't know how easy it would be. There could be an easier way to get a TEM sample.
That's all I can think of right now.
Good luck.
-Lyle
-- Lyle Gordon Department of Materials Science and Engineering Northwestern University
On Wed, Mar 18, 2009 at 8:57 AM, {giulio.lamedica-at-libero.it} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both giulio.lamedica-at-libero.it as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: giulio.lamedica-at-libero.it } Name: Giulio } } Organization: Univ of Rome La Sapienza } } Title-Subject: [Filtered] TiO2 50 100nm nanoparticles in a liquid suspension } } Question: I'm interested in analysing TiO2 50 100nm nanoparticles } in a liquid suspension with FE-SEM. } I'm intereted in particles characterization, because we know exactly } their shape. } What I'd like to investigate is how they are aggregate. } Of course if we dry them we change their aggregation state. Have you } any ideas to suggest? } } Thanks } } Giulio } } } } Login Host: 82.191.37.58 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 11 -- From zaluzec-at-microscopy.com Wed Mar 18 08:43:14 2009 } 10, 11 -- Received: from [206.69.208.22] (msdvpn072.msd.anl.gov [130.202.238.72]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IDh8P7007648 } 10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 08:43:11 -0500 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240800c5e6a9345c1a-at-[206.69.208.22]} } 10, 11 -- Date: Wed, 18 Mar 2009 08:43:06 -0500 } 10, 11 -- To: microscopy-at-microscopy.com } 10, 11 -- From: giulio.lamedica-at-libero.it (by way of MicroscopyListserver) } 10, 11 -- Subject: viaWWW: TiO2 50 100nm nanoparticles in a liquid suspension } 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 13, 36 -- From lgordon-at-gmail.com Wed Mar 18 11:25:34 2009 13, 36 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.248]) 13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IGPWJY021029 13, 36 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:25:33 -0500 13, 36 -- Received: by an-out-0708.google.com with SMTP id b38so89938ana.0 13, 36 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 09:25:29 -0700 (PDT) 13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 36 -- d=gmail.com; s=gamma; 13, 36 -- h=domainkey-signature:mime-version:received:in-reply-to:references 13, 36 -- :date:message-id:subject:from:to:content-type 13, 36 -- :content-transfer-encoding; 13, 36 -- bh=fIaxaDc+wIHyVP9DlOlM6Xl+P/H7eKMGpGJIWms6hLA=; 13, 36 -- b=T2jEQJlFahKiv+1MpxYne1tzXg726XvR/SWSrzLLTU0TWe+7iBRsIIwq90Emss0IU1 13, 36 -- q3NYYr4x0Fv1lswjATBW8/sZYcsgSQg4N3QcepNHj6RKmzKzJR0lAH0zkZw1v4nG7/11 13, 36 -- A/VykP3o967ysCrDxxhNIPdseZhN3DpeksTng= 13, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 36 -- d=gmail.com; s=gamma; 13, 36 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 13, 36 -- :content-type:content-transfer-encoding; 13, 36 -- b=De9hStseVzHaMaVdOaUeAgWWJd1lRTk2mmVZ68wO2aLrR1LUNGGqcb5U5eInJ5Ja8Z 13, 36 -- BqEDXp9pcs/xuxXhafCI8Vhz0qL7XDlJK8OMSbQchwnWTK9erIFCqs9Uw7k7taz03uLx 13, 36 -- MgtkhiVKmFcSSaDtKf+hHgserhtplm1cYDsVM= 13, 36 -- MIME-Version: 1.0 13, 36 -- Received: by 10.101.70.14 with SMTP id x14mr1691423ank.131.1237393529097; Wed, 13, 36 -- 18 Mar 2009 09:25:29 -0700 (PDT) 13, 36 -- In-Reply-To: {200903181357.n2IDveBx021692-at-ns.microscopy.com} 13, 36 -- References: {200903181357.n2IDveBx021692-at-ns.microscopy.com} 13, 36 -- Date: Wed, 18 Mar 2009 11:25:29 -0500 13, 36 -- Message-ID: {88f183960903180925k551063a1lc5996951a116320c-at-mail.gmail.com} 13, 36 -- Subject: Re: [Microscopy] viaWWW: TiO2 50 100nm nanoparticles in a liquid 13, 36 -- suspension 13, 36 -- From: Lyle Gordon {lgordon-at-gmail.com} 13, 36 -- To: giulio.lamedica-at-libero.it, microscopy-at-microscopy.com 13, 36 -- Content-Type: text/plain; charset=ISO-8859-1 13, 36 -- Content-Transfer-Encoding: 8bit 13, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2IGPWJY021029 ==============================End of - Headers==============================
Actually, the answer is in the original description of the specimens "from an HIV positve patient". Unfortunately the preparation in Histogel has caused some deterioration in quality of specimen at the end point. What you are seeing is lentivirus particles. given the specimen source you can assume that they are the human version - ie HIV particles.
paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Mar 18 11:44:10 2009 6, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IGi8D4002743 6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:44:10 -0500 6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 6, 20 -- (authenticated bits=0) 6, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n2IGi5lZ013388; 6, 20 -- Wed, 18 Mar 2009 11:44:05 -0500 (CDT) 6, 20 -- Message-ID: {49C124D4.8050302-at-umanitoba.ca} 6, 20 -- Date: Wed, 18 Mar 2009 11:44:04 -0500 6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: dsoren-at-umich.edu 6, 20 -- Subject: Re: [Microscopy] Re: What is this critter? 6, 20 -- References: {200903181530.n2IFUrHM008486-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200903181530.n2IFUrHM008486-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
Hello, What wavelengths are labs using for IR filters used with IR-DIC such as with patch clamping? We have a microscope on an old, heavily used, patch clamp rig with a badly degraded IR filter. Dodt and Zeiglgansberger, 1997 cite a Schott filter with 780 nm maximum, Omega sells a 780/40, Chroma offers a Schott glass filter } 780. This microscope has no documentation for the filter and none of the vendors I've contacted recognized the part number on the filter.
Thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
****************************************************************************** The box said "Requires WindowsXP or better", so I bought a Macintosh. ******************************************************************************
==============================Original Headers============================== 7, 24 -- From glenmac-at-u.washington.edu Wed Mar 18 12:03:05 2009 7, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IH351X016969 7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 12:03:05 -0500 7, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.141] (may be forged)) 7, 24 -- by mxout1.cac.washington.edu (8.14.3+UW09.02/8.14.3+UW09.01) with ESMTP id n2IH342S028861 7, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:03:04 -0700 7, 24 -- X-Auth-Received: from D-128-95-178-135.dhcp4.washington.edu (D-128-95-178-135.dhcp4.washington.edu [128.95.178.135]) 7, 24 -- (authenticated authid=glenmac) 7, 24 -- by smtp.washington.edu (8.14.3+UW09.02/8.14.3+UW09.01) with ESMTP id n2IH34Hq021937 7, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:03:04 -0700 7, 24 -- Message-Id: {D8E8FA16-97C5-44A5-BC56-6BD6D8949849-at-u.washington.edu} 7, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 7, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 7, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3) 7, 24 -- Subject: IR filters for DIC 7, 24 -- Date: Wed, 18 Mar 2009 10:03:04 -0700 7, 24 -- X-Mailer: Apple Mail (2.930.3) 7, 24 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.18.164927 7, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=8%, Report='REPLICA_BRANDS 0.05, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_900_999 0, TO_NO_NAME 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
I agree with Paul as to it being a virus... If not herpes than possibly a paramyxovirus virus such as measles that also is an enveloped virus that buds from the cytoplasmic membrane. I am curious as the the spikey stuff all around. Was a phosphate buffer used at some point prior to UA en-bloc staining? This can cause formation of phosphate crystals that look very much like what you have. If this was not present than you could probably more clearly see the formation of the virus as it buds from the cytoplasmic membrane. -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: Paul Chipman {paulrc-at-bilbo.bio.purdue.edu} } Reply-To: Paul Chipman {paulrc-at-bilbo.bio.purdue.edu} } Date: Wed, 18 Mar 2009 10:31:10 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Re: What is this critter? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It looks like a large icosahedral virus and is similar in size to } Herpesvirus. There are not many large (} 100nm) icosahedral viruses that } infect vertebrates but I'd still suggest you consider it. The third } image has one very clear particle in the cytoplasm and near the top of } the same image, a number of particles that look to be budding. } -paul } } } -- } Paul Chipman } Director, Biological Electron Microscopy Facility } Purdue University } 765-494-1487 } } } Quoting dsoren-at-umich.edu: } } -------------------------------------------------------------- } } } } Dear Listers, } } } } I am writing to enlist your help in identifying the organism in these } } TEM images, which } } you can view by going to this link. } } } } http://www.med.umich.edu/cdb/mil/docs/temimages.html } } } } It was isolated from macrophages from the peripheral blood of } } sarcoidosis patients. The same organism was also isolated from } } the macrophages of a pleural effusion from an HIV positve patient } } with diffuse large B cell lymphoma after many months of culture. It } } was fixed in glutaraldehyde in Sorensens buffer,pelleted, and } } embedded in Histogel. Then the Histogel-embedded pellet was } } prepared for TEM by standard techniques, i.e., post fixed with osmium, } } en bloc stained with uranyl acetate, dehydrated in a graded series of } } ethanol followed by propylene oxide, and infiltrated and embedded in } } Epon. Ultra-thin sections were post stained with uranyl acetate and lead } } citrate. } } } } Has anyone seen anything like this? We would like to give it its } } proper name instead of its current name, The Critter. Any help you } } might give us would be appreciated. } } } } Thanks, } } } } Dotty Sorenson } } } } } } Dorothy Sorenson } } Microscopy and Image-analysis Laboratory } } Department of Cell and Developmental Biology } } University Of Michigan Medical School } } A807 BSRB } } 109 Zina Pitcher Place } } Ann Arbor, MI 48109-2200 } } (734)763-1170 } } FAX (734)763-1166 } } } } } ==============================Original Headers============================== } 7, 20 -- From paulrc-at-bilbo.bio.purdue.edu Wed Mar 18 10:27:42 2009 } 7, 20 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu } [128.210.5.131]) } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n2IFRe2O005496 } 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 10:27:40 -0500 } 7, 20 -- Received: from dhcp18-69-fixed.bio.purdue.edu } (dhcp18-69-fixed.bio.purdue.edu [128.210.18.69]) } 7, 20 -- (authenticated bits=0) } 7, 20 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-auth.purdue.edu) } with ESMTP id n2IFRcIC000907 } 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:27:38 -0400 } 7, 20 -- Message-ID: {49C112EA.6060703-at-bilbo.bio.purdue.edu} } 7, 20 -- Date: Wed, 18 Mar 2009 11:27:38 -0400 } 7, 20 -- From: "Chipman, Paul R" {paulrc-at-bilbo.bio.purdue.edu} } 7, 20 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) } 7, 20 -- MIME-Version: 1.0 } 7, 20 -- To: microscopy-at-microscopy.com } 7, 20 -- Subject: Re: [Microscopy] What is this critter? } 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 20 -- Content-Transfer-Encoding: 7bit } 7, 20 -- X-PMX-Version: 5.4.0.320885 } 7, 20 -- X-PerlMx-Virus-Scanned: Yes } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 30 -- From dsherman-at-purdue.edu Wed Mar 18 12:08:35 2009 4, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IH8Ytn028628 4, 30 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 12:08:34 -0500 4, 30 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 4, 30 -- by mailhub129.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n2IH8YfL003579 4, 30 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 13:08:34 -0400 4, 30 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 4, 30 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n2IH8YgN005066 4, 30 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 13:08:34 -0400 4, 30 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 4, 30 -- Wed, 18 Mar 2009 13:08:33 -0400 4, 30 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 4, 30 -- Wed, 18 Mar 2009 17:07:45 +0000 4, 30 -- User-Agent: Microsoft-Entourage/12.15.0.081119 4, 30 -- Date: Wed, 18 Mar 2009 13:07:43 -0500 4, 30 -- Subject: Re: [Microscopy] Re: What is this critter? 4, 30 -- From: Debby Sherman {dsherman-at-purdue.edu} 4, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 4, 30 -- Message-ID: {C5E6A29F.3BDA4%dsherman-at-exchange.purdue.edu} 4, 30 -- Thread-Topic: [Microscopy] Re: What is this critter? 4, 30 -- Thread-Index: Acmn7A0PiVsm07dpQ2K0VEUXHU2gOA== 4, 30 -- In-Reply-To: {200903181531.n2IFVALC008783-at-ns.microscopy.com} 4, 30 -- Mime-version: 1.0 4, 30 -- Content-type: text/plain; 4, 30 -- charset="US-ASCII" 4, 30 -- Content-transfer-encoding: 7bit 4, 30 -- X-OriginalArrivalTime: 18 Mar 2009 17:08:33.0980 (UTC) FILETIME=[2B7247C0:01C9A7EC] 4, 30 -- X-PMX-Version: 5.4.0.320885 4, 30 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Does anyone know if it is or isn't possible to fit CL to a JEOL 840 which already has 3 x WDS and 1 x EDS as well as the standard 840 Optical Microscope?
There's not a lot of room left in there, but maybe something involving fibre optics or the OM might fit in.
What if there was only the EDS and the OM ie no WDS?
Vendors' replies welcome.
cheers
Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 9, 28 -- From r.sims-at-auckland.ac.nz Wed Mar 18 13:42:43 2009 9, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IIgcup014811 9, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 13:42:42 -0500 9, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 686EA9DC35 9, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 07:42:37 +1300 (NZDT) 9, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 9, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 9, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 9, 28 -- with ESMTP id OCbPxNLcvdQc for {microscopy-at-Microscopy.Com} ; 9, 28 -- Thu, 19 Mar 2009 07:42:37 +1300 (NZDT) 9, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 9, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) 9, 28 -- (No client certificate requested) 9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 48D739DB52 9, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 07:42:37 +1300 (NZDT) 9, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 9, 28 -- To: microscopy-at-Microscopy.Com 9, 28 -- Date: Thu, 19 Mar 2009 07:44:51 +1300 9, 28 -- MIME-Version: 1.0 9, 28 -- Subject: CL for JXA-840A? 9, 28 -- Message-ID: {49C1F7F3.14890.AC737-at-r.sims.auckland.ac.nz} 9, 28 -- Priority: normal 9, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 28 -- Content-type: text/plain; charset=US-ASCII 9, 28 -- Content-transfer-encoding: 7BIT 9, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
     Recently I have started to work on Convergent Beam Electron Diffraction. So I have very basic questions. Please help me in gaining knowledge in the related field.
My first goal is to determine the thickness of MAGICAL sample using CBED. Here are my problems.
1. When I was taking a CBED pattern at lower symmetry, I was unable to find any K-M fringes on central spot {000} . I have varied camera length from 30cm to 300cm and also  changed exposure time from 0.1 sec to 30 sec. I was neither able to find any fringes on the central spot nor was able to find spot next to it.
    Basically to find thickness, we mainly require 2 spots next to each other. One is central spot and other one is {220} or {200} of CBED pattern. I was unable to get these 2 spots next to each other. Please tell me what are the variables need to be changed in JEOL 2011 TEM so that we get a pattern where spots are nearby to each other.
2.  According to Williams and Carter text book, to get KM fringes, angle of convergence (2α) should be less than Bragg’s angle (2θ). Can you specify how can we change angle of convergence and Bragg’s angle in JEOL 2011??? If I’m correct, angle of convergence is alpha selector and Bragg’s angle is magnification toggle of JEOL 2011??? If I’m wrong, please correct me.
3. Right now I’m using accelerating voltage of 200kv. Does it have any impact on CBED patterns once if I change it to 100kv or 150kv???
Thanks for your time
Vishnu Mogili,
PhD student, Materials & Surface Science Institute, University of Limerick, Ireland.
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What's the cheapest way of getting into CL of quartz? Can CL be put onto a benchtop SEM?
cheers
Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
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Title-Subject: [Filtered] Don't Miss this Opportunity -- AMFA 2009, April 2-3, Santa Clara
Question: Dear colleagues,
In just two weeks, an impressive line-up of speakers will discuss "enabling and disruptive technologies" at the multidisciplinary Advanced Materials/ Failure Analysis (AMFA) Workshop - www.amfaworkshop.org.
This is a one-day workshop following a day of educational courses and a 4 hour workshop on government funding for failure analysis. The registration fee is nominal, and the 2009 program continues the AMFA tradition of new information presented by leading-edge researchers. This is an opportunity you won't want to miss. The workshop on government funding for FA is free, but invitation only - please contact me if you wish an invitation. --------------------------------------------------------------------------------------------------------------
The 2009 AMFA Workshop is financially sponsored by Omniprobe, Inc., FEI Company, and ARC Technologies. Technical co-sponsors include the IEEE Reliability Society and the Electronic Device Failure Analysis Society. Members of the societies receive a registration discount.
PROGRAM This year's exciting program consists of renowned invited speakers covering a variety of enabling and disruptive topics in a series of 40 minute presentations, each followed by 20 minutes of facilitator-led audience discussion.
Ed Principe - Zeiss, Inc. Nanotomography and Applications of Stereolithography
Joseph Michael - Sandia National Laboratories What's New in Electrons, Ions and X-rays
Dieter Schroder - Arizona State University Characterization of Nano Devices and Nano Characterization of Materials
Kevin Hemker - John Hopkins University Lilliputian techniques for characterizing mechanical properties
William Vanderlinde - Laboratory for Physical Sciences Government funding for FA tool development
Jerry Mearini -Teraphysics Corporation Present and Near-Future Detection and Identification Capabilities of Terahertz Technologies
Kevin Bennett - Arizona State University Structural and molecular magnetic resonance microscopy: a biological perspective
On site registration will be available. Please visit www.amfaworkshop.org for details. We look forward to seeing you.
Best Regards, Cheryl Hartfield AMFA 2009 Committee Member
1. If you are unable to see K-M fringes in your CBED disks, it may be that your specimen is too thin. The number of fringes increases with thickness and where your thickness is less than about half an extinction distance (for the reflection you are using Si(220) E=96nm at 200kV - so {50nm and you may get nothing. Similarly if your specimen is exceedingly thick and you aren't using an energy filter, you might find the fringes are wiped out. Also if you have a large variation in thickness in the region probed, the fringes will blur into each other. Choose a very flat region of specimen.
Check out J. Microsc 224 (2006) 187-196, where I describe some thickness by CBED experiments with silicon and P91 on a JEOL 2010. This work describes use of Vincent Hou's excellent DigitalMicrograph script for carrying out the thickness calculation. If you are capturing images using DigitalMicrograph, you can install this script - it makes the calculation a breeze - get it (Thickness by CBED) from the DigitalMicrograph Script Database (URL at the bottom of this message).
Thickness determination is achieved by setting up two beam conditions - the (000) transmitted spot and another diffracted beam are intense - not two diffracted beams as your post suggests (to me). The choice of which beam to use isn't too important, but since the extinction distance varies with the reflection, then the minimum thickness you can measure is determined by your choice of diffracted beam (for the reasons mentioned in 1). It's best to use low index reflections - high index reflection have longer extinction distances. You only need to measure the fringe spacing within the diffracted beam for the thickness calculation. However, in order to convert this distance measurement into an angle, you need the transmitted beam present, since the distance from the edge of the transmitted beam to the edge of the diffracted beam corresponds to the angle 2theta (Bragg equation) which gives you the distance to angle calibration. To minimise measurement error capture the patterns at a camera length such that the spots span a large proportion of the screen.
2. Set up your CBED conditions so that the transmitted (000) and diffracted beams are large enough to almost touch. The larger they are the smaller the measurement error will be. However, if they overlap, you may find making the disk edge to disk edge measurement difficult. Experiment with both the alpha control on your JEOL and also the condenser aperture to understand how these affect your CBED pattern.
3. Changing the microscope voltage will change your wavelength, and since wavelength appears in the Bragg equation (nL=2dsin(theta)), your Bragg angle (half the disk edge to disk edge) distance will change (your patterns are bigger at lower voltage for a given camera length). Also, the extinction distance - which appears in the thickness equation will change. However, from a practical perspective the measurement you make will be correct at any voltage, provided you measure the fringe spacing and disk edge to disk edge distance correctly and you supply the correct extinction distance (ie don't use the 200kV value if you are working at lower voltage).
Finally the CBED method is accurate but time consuming. If you have an energy filter, thickness mapping is much easier to do. However, you do need a good value for the mean free path in order to convert your map into true thickness (see the earlier reference on how to measure the mean free path). There is also a DigitalMicrograph script which will help you estimate the mean free path (Mean Free Path Estimator). It is described in the reference I gave you, and today I have posted a much improved version of it to DM Script Database ( http://www.felmi-zfe.tugraz.at/dm_scripts/welcome.html) - it may take a week or so to appear.
Regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
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This is an invitation to you to a brand new electron microscopy community web site: http://www.emfocal.com
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From antonio.mora.pi-at-terra.com Thu Mar 19 01:19:52 2009 Return-Path: {antonio.mora.pi-at-terra.com} Received: from google.com (eatkyo586106.adsl.ppp.infoweb.ne.jp [202.233.193.106]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J6Jomb027341 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 19 Mar 2009 01:19:51 -0500 Received: from [187.156.23.93] (HELO google.com) by foggy-sneak.net; Thu, 19 Mar 2009 15:13:31 +0900
Dear ...,
looking at your below linked site, I fail completely to see who is behind that. An individual, a company? On your home page you say "I", in the policy it says "we" and "our". Who? The content of this page goes against zero at the moment, but the policy makes clear that "you" will hold the right on everything published unless otherwise noted by the contributor. You also intend to benefit from commercial advertizements: "Please contact us if you wish to support this site through advertisements." With the anonymity practiced, I have the strong feeling that this site intends to make profit from the knowledge of our community and our willingness to share it. I personally think there are better places to practice this (e.g. this list server).
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Please respond to Subject samwarren-at-emfocal [Microscopy] TEM - Inviation to a .com new EM community web site
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This is an invitation to you to a brand new electron microscopy community web site: http://www.emfocal.com
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In water I have never seen TiO2 particles aggregate. Now to answer your question: - You only need several particles to aggregate in order to see it in light microscopy. Why bother with EM? - Just filter a suspension of particles! I cannot recommend you enough the anopore filters. They are simply fantastic!
Per altre domande non esitare a scrivermi.
Best regards,
Stephane
----- Original Message ---- X-from: "giulio.lamedica-at-libero.it" {giulio.lamedica-at-libero.it} To: nizets2-at-yahoo.com Sent: Wednesday, March 18, 2009 2:51:20 PM
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Email: giulio.lamedica-at-libero.it Name: Giulio
Organization: Univ of Rome La Sapienza
Title-Subject: [Filtered] TiO2 50 100nm nanoparticles in a liquid suspension
Question: I'm interested in analysing TiO2 50 100nm nanoparticles in a liquid suspension with FE-SEM. I'm intereted in particles characterization, because we know exactly their shape. What I'd like to investigate is how they are aggregate. Of course if we dry them we change their aggregation state. Have you any ideas to suggest?
Definitely viral particles. I would suggest to use protocols to purify virus particles from cells in culture (pretty easy). Then you can do whatever you want to identify them if you need/want to (PCR, ELISA...).
Stéphane
----- Original Message ---- X-from: "paul_hazelton-at-umanitoba.ca" {paul_hazelton-at-umanitoba.ca} To: nizets2-at-yahoo.com Sent: Wednesday, March 18, 2009 5:48:39 PM
Dotty, et al
Actually, the answer is in the original description of the specimens "from an HIV positve patient". Unfortunately the preparation in Histogel has caused some deterioration in quality of specimen at the end point. What you are seeing is lentivirus particles. given the specimen source you can assume that they are the human version - ie HIV particles.
paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 6, 20 -- From paul_hazelton-at-umanitoba.ca Wed Mar 18 11:44:10 2009 6, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2IGi8D4002743 6, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 Mar 2009 11:44:10 -0500 6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 6, 20 -- (authenticated bits=0) 6, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n2IGi5lZ013388; 6, 20 -- Wed, 18 Mar 2009 11:44:05 -0500 (CDT) 6, 20 -- Message-ID: {49C124D4.8050302-at-umanitoba.ca} 6, 20 -- Date: Wed, 18 Mar 2009 11:44:04 -0500 6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: dsoren-at-umich.edu 6, 20 -- Subject: Re: [Microscopy] Re: What is this critter? 6, 20 -- References: {200903181530.n2IFUrHM008486-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200903181530.n2IFUrHM008486-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 21, 24 -- From nizets2-at-yahoo.com Thu Mar 19 06:53:44 2009 21, 24 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237]) 21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2JBripX024052 21, 24 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 06:53:44 -0500 21, 24 -- Received: (qmail 1684 invoked by uid 60001); 19 Mar 2009 11:53:44 -0000 21, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237463623; bh=U+D+lcVtgKRTB7sr2D+yajw8UP/A9hyN9nQBqk4SCJA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=0aGXotpWnSboDeLkDcg6pklo7wwNtD7T8C+Nbd1nYQRX03g8uitRXaWtsJY8kr+8HsCZAKJfFbgGuOlohwNsbeTxzk/BVpig9QKVMNJhVwaNdEiJQWISmcUUeX5vkFKY4v6O3oipGahe1j1MRnXi5C1fCKC+QyjGmhZiUYpRqN4= 21, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 21, 24 -- s=s1024; d=yahoo.com; 21, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 21, 24 -- b=rBfBlIYR0RSjqJP4UAHTNmtErP+8B17lq2GjmCVZCL84oHzIyJ+rXIgzAuirwDmbAWYA8D6tJs6pUHV0VWq4Ozx+E/1ynutDBReuwl74OnUpFSSjQ/MwbT1lGvrZXpBkcji7rv2i+JhD0C+KnrTOooJSjDSJEUGbx/CmCftbew4=; 21, 24 -- Message-ID: {913802.715.qm-at-web110814.mail.gq1.yahoo.com} 21, 24 -- X-YMail-OSG: nHqaGNEVM1mA9jOpqRFNub8hyBjnn5irNtsQ4o1.XKTjfqJhJQ7OI0xu.fHOqNqBEhHzSba4NzN7Kpmfvn76Ax77z8ZVaWR2sMMetMbqYOOiQ.TTubfdsWQZ2Um9u360FPR1FDXAyL5.WMJnsYVPkhisw4MtVo4FY1mPd65uMX_Nskv50Cn_5J7BlNh14WnN8nEqPAT7sA5HDhgJVJ0MxSAZz7sJCi_eMppWwvgc_HzjPBKVrzxw48XhnsP9zNJ9cTwQlnJ.0a2donZQ8D9QLz5E865.RMW6qg-- 21, 24 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Thu, 19 Mar 2009 04:53:43 PDT 21, 24 -- X-Mailer: YahooMailRC/1277.29 YahooMailWebService/0.7.289.1 21, 24 -- References: {200903181648.n2IGmdJO009326-at-ns.microscopy.com} 21, 24 -- Date: Thu, 19 Mar 2009 04:53:43 -0700 (PDT) 21, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com} 21, 24 -- Subject: Re: [Microscopy] What is this critter? 21, 24 -- To: microscopy-at-microscopy.com 21, 24 -- In-Reply-To: {200903181648.n2IGmdJO009326-at-ns.microscopy.com} 21, 24 -- MIME-Version: 1.0 21, 24 -- Content-Type: text/plain; charset=iso-8859-1 21, 24 -- Content-Transfer-Encoding: 8bit 21, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JBripX024052 ==============================End of - Headers==============================
Completely agree. No greetings and no signature. I don't go further, not even to the point of clicking on the link.
Stephane
----- Original Message ---- X-from: "petra.wahlbring-at-goodyear.com" {petra.wahlbring-at-goodyear.com} To: nizets2-at-yahoo.com Sent: Thursday, March 19, 2009 9:41:24 AM
Dear ...,
looking at your below linked site, I fail completely to see who is behind that. An individual, a company? On your home page you say "I", in the policy it says "we" and "our". Who? The content of this page goes against zero at the moment, but the policy makes clear that "you" will hold the right on everything published unless otherwise noted by the contributor. You also intend to benefit from commercial advertizements: "Please contact us if you wish to support this site through advertisements." With the anonymity practiced, I have the strong feeling that this site intends to make profit from the knowledge of our community and our willingness to share it. I personally think there are better places to practice this (e.g. this list server).
Petra __________________________________ Dr. Petra Wahlbring Lead Engineer Analytical Test Lab Goodyear S.A. Technical Center Colmar-Berg, Luxembourg e-mail: petra.wahlbring-at-goodyear.com phone: +352 8199 3725 or GTN 631 3725 fax: +352 8199 5643 - May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the express written consent of The Goodyear Tire & Rubber Company. -
                                           samwarren-at-emfocal                            .com                                                                 To       03/19/09 04:33 AM    petra.wahlbring-at-goodyear.com                                        cc                                            Please respond to                  Subject       samwarren-at-emfocal    [Microscopy] TEM - Inviation to a          .com        new EM community web site                                                                                                                                                                                                                                  Â
The Microscopy ListServer -- CoSponsor:Â The Microscopy Society of America
This is an invitation to you to a brand new electron microscopy community web site: http://www.emfocal.com
This site focuses on information sharing and community. Our intention on this site is to make it a “wikipedia-like†information repository: a user collaborated information base about anything related to electron microscopy, micro-analysis, applications, …
I invite you come to take a look of the site. If you like it, help us to build it.
Feel free to forward this message.
Thank you,
==============================Original Headers============================== 7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009 7, 31 -- Received: from outbound-mail-357.bluehost.com (outbound-mail-357.bluehost.com [66.147.249.251]) 7, 31 --Â Â Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2J3VJln009306 7, 31 --Â Â Â Â Â for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 22:31:19 -0500 7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37 -0000 7, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161) 7, 31 --Â by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar 2009 03:26:37 -0000 7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com; 7, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
7, 31 --Â Â Â Â Â b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby +gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2;
7, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com) 7, 31 --Â Â Â Â Â by host361.hostmonster.com with esmtpa (Exim 4.69) 7, 31 --Â Â Â Â Â (envelope-from {samwarren-at-emfocal.com} ) 7, 31 --Â Â Â Â Â id 1Lk8yV-0006fA-BN 7, 31 --Â Â Â Â Â for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19 -0600 7, 31 -- Received: from 124.192.231.32 ([124.192.231.32]) 7, 31 --Â Â Â Â (SquirrelMail authenticated user samwarren-at-emfocal.com) 7, 31 --Â Â Â Â by host361.hostmonster.com with HTTP; 7, 31 --Â Â Â Â Wed, 18 Mar 2009 21:31:19 -0600 (MDT) 7, 31 -- Message-ID: {1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com} 7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT) 7, 31 -- Subject: TEM - Inviation to a new EM community web site 7, 31 -- From: samwarren-at-emfocal.com 7, 31 -- To: Microscopy-at-Microscopy.Com 7, 31 -- User-Agent: SquirrelMail/1.4.13 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain;charset=iso-8859-1 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-Priority: 3 (Normal) 7, 31 -- Importance: Normal 7, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server} ==============================End of - Headers==============================
==============================Original Headers============================== 25, 17 -- From petra.wahlbring-at-goodyear.com Thu Mar 19 03:35:47 2009 25, 17 -- Received: from emeam1.goodyear.com (emeam1.goodyear.com [57.67.226.7]) 25, 17 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J8ZkcU030439 25, 17 -- Â Â Â for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 03:35:47 -0500 25, 17 -- In-Reply-To: {200903190333.n2J3XnG8014706-at-ns.microscopy.com} 25, 17 -- Subject: Re: [Microscopy] TEM - Inviation to a new EM community web site 25, 17 -- To: microscopy-at-microscopy.com 25, 17 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 25, 17 -- Message-ID: {OFBA11D716.1615D217-ONC125757E.002D858E-C125757E.002F2C9E-at-goodyear.com} 25, 17 -- From: petra.wahlbring-at-goodyear.com 25, 17 -- Date: Thu, 19 Mar 2009 09:35:16 +0100 25, 17 -- X-MIMETrack: Serialize by Router on ECLNGM01/EU/GDYRNET(Release 8.0.1|February 07, 2008) at 25, 17 --Â 03/19/2009 09:35:51 AM 25, 17 -- MIME-Version: 1.0 25, 17 -- Content-type: text/plain; charset=UTF-8 25, 17 -- Content-Transfer-Encoding: 8bit 25, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2J8ZkcU030439 ==============================End of - Headers==============================
==============================Original Headers============================== 40, 24 -- From nizets2-at-yahoo.com Thu Mar 19 06:57:39 2009 40, 24 -- Received: from web110803.mail.gq1.yahoo.com (web110803.mail.gq1.yahoo.com [67.195.13.226]) 40, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2JBvc8U002543 40, 24 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 06:57:39 -0500 40, 24 -- Received: (qmail 53358 invoked by uid 60001); 19 Mar 2009 11:57:38 -0000 40, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237463858; bh=ffKLAzvStBZJyCetr/0aw2VqXbo9Qe0ZyLnpW1hhIOI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=26vs9y9kG0malYz3m8EeP73F5h8AdEiX8/ym7Lh2Q66MJ3Go5x19lRaiv/MsVLDw9cXWsTqsQIralSBSQ7Xpodi1ysOojN8GT54DXT78rDHP0Md3i5oFxbEJKpWQ3HTTr9r5cTv5RCWP/1AXB9+yTmSDtP12xZHKVHa2RfSYyIU= 40, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 40, 24 -- s=s1024; d=yahoo.com; 40, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 40, 24 -- b=AznRG5wp5FzXl+unI8f1VbpmBnH1rdk7m9YxxHcFVEHSmMaAZtmGEbp+ipWJMHbXQn0uPeK77+A6sMa+8XiQvujvXErxvMfedgPqTedDiKWm6BkAuqV/FQXx4Lw3Oe14NeacdKVoKOLsdA3sNqJ0YDBkJdS5wrb0izxstWvlkI4=; 40, 24 -- Message-ID: {404152.53062.qm-at-web110803.mail.gq1.yahoo.com} 40, 24 -- X-YMail-OSG: JfVStY8VM1lUfslScPGCni1SKPjHUQZjwYJUHJI0iETc2GsV3yKkh9nxGqxHapoPHnSOYiBuExSmyFx7Gus7YRiwTGdKQfJ5PsTg7_n.DhENvuWG9Z_EaOaBff7v55BW6d2zKUjDCLiX0TVkUoD9WxRGeGSlfc_t9SYVoXK0.5m8rkV8vpghotNk6rxTfIKN_yJPcPh9L9GfNx_mAiSTawVlQzvWTnKYGz1ZK7X0xLRCm95wxh4oCHl6p7BActOh0u3wTK50_XdNlKQ3HKCf0Uw- 40, 24 -- Received: from [80.122.101.100] by web110803.mail.gq1.yahoo.com via HTTP; Thu, 19 Mar 2009 04:57:38 PDT 40, 24 -- X-Mailer: YahooMailRC/1277.29 YahooMailWebService/0.7.289.1 40, 24 -- References: {200903190841.n2J8fO5c004588-at-ns.microscopy.com} 40, 24 -- Date: Thu, 19 Mar 2009 04:57:38 -0700 (PDT) 40, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com} 40, 24 -- Subject: Re: [Microscopy] Re: TEM - Inviation to a new EM community web site 40, 24 -- To: microscopy-at-microscopy.com 40, 24 -- In-Reply-To: {200903190841.n2J8fO5c004588-at-ns.microscopy.com} 40, 24 -- MIME-Version: 1.0 40, 24 -- Content-Type: text/plain; charset=utf-8 40, 24 -- Content-Transfer-Encoding: 8bit 40, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JBvc8U002543 ==============================End of - Headers==============================
I decided to click. The legalese seems to simply recognize the fact that once you've put something on the web, it's there for everyone to use. Seems pretty straight-forward.
On the other hand, who is Sam Warren? It sounds like maybe I could do a search of Harvard's website and maybe find out, but why bother when nothing is offered for contact information concerning this "group" or "organization"? Does Harvard even know what he/they are up to? Sounds like he/they are concerned enough about being shut down to cover their derrieres, but don't want anyone else to have a clue as to who he/they are or what he/they are up to.
Right. I won't be back.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, March 19, 2009 8:00 AM To: kenconverse-at-qualityimages.biz
Completely agree. No greetings and no signature. I don't go further, not even to the point of clicking on the link.
Stephane
----- Original Message ---- X-from: "petra.wahlbring-at-goodyear.com" {petra.wahlbring-at-goodyear.com} To: nizets2-at-yahoo.com Sent: Thursday, March 19, 2009 9:41:24 AM
Dear ...,
looking at your below linked site, I fail completely to see who is behind that. An individual, a company? On your home page you say "I", in the policy it says "we" and "our". Who? The content of this page goes against zero at the moment, but the policy makes clear that "you" will hold the right on everything published unless otherwise noted by the contributor. You also intend to benefit from commercial advertizements: "Please contact us if you wish to support this site through advertisements." With the anonymity practiced, I have the strong feeling that this site intends to make profit from the knowledge of our community and our willingness to share it. I personally think there are better places to practice this (e.g. this list server).
Petra __________________________________ Dr. Petra Wahlbring Lead Engineer Analytical Test Lab Goodyear S.A. Technical Center Colmar-Berg, Luxembourg e-mail: petra.wahlbring-at-goodyear.com phone: +352 8199 3725 or GTN 631 3725 fax: +352 8199 5643 - May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the express written consent of The Goodyear Tire & Rubber Company. -
The Microscopy ListServer -- CoSponsor:Â The Microscopy Society of America
This is an invitation to you to a brand new electron microscopy community web site: http://www.emfocal.com
This site focuses on information sharing and community. Our intention on this site is to make it a “wikipedia-like†information repository: a user collaborated information base about anything related to electron microscopy, micro-analysis, applications, …
I invite you come to take a look of the site. If you like it, help us to build it.
Feel free to forward this message.
Thank you,
==============================Original Headers============================== 7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009 7, 31 -- Received: from outbound-mail-357.bluehost.com (outbound-mail-357.bluehost.com [66.147.249.251]) 7, 31 --Â Â Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2J3VJln009306 7, 31 --Â Â Â Â Â for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 22:31:19 -0500 7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37 -0000 7, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161) 7, 31 --Â by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar 2009 03:26:37 -0000 7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com; 7, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User;
This site is registered to Shixin Wang. Only reference I found was for a post doc at U. Mich., but the site indicated that the fellowship ended in 2000, so I can not say for sure whether it is the same person, or not.
Not sure who Sam is, or if you should trust him. Probably not, I do not think he uses a spell checker.
Steve
Steven Lee Chief Technologist Electron Microscopy Laboratory Baylor University Medical Center 3500 Gaston Ave Dallas, TX 75246 6 214.820.3302 Ê 214.820.4110 2 stevenle-at-baylorhealth.edu
-----Original Message----- X-from: samwarren-at-emfocal.com [mailto:samwarren-at-emfocal.com] Sent: Wednesday, March 18, 2009 10:35 PM To: Lee, Steven
This is an invitation to you to a brand new electron microscopy community web site: http://www.emfocal.com
This site focuses on information sharing and community. Our intention on this site is to make it a "wikipedia-like" information repository: a user collaborated information base about anything related to electron microscopy, micro-analysis, applications, ...
I invite you come to take a look of the site. If you like it, help us to build it.
Feel free to forward this message.
Thank you,
==============================Original Headers============================== 7, 31 -- From samwarren-at-emfocal.com Wed Mar 18 22:31:20 2009 7, 31 -- Received: from outbound-mail-357.bluehost.com (outbound-mail-357.bluehost.com [66.147.249.251]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2J3VJln009306 7, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 22:31:19 -0500 7, 31 -- Received: (qmail 18739 invoked by uid 0); 19 Mar 2009 03:26:37 -0000 7, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161) 7, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 19 Mar 2009 03:26:37 -0000 7, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com; 7, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User; 7, 31 -- b=OIUeaTgilIYWRRZ3obeoAlOwTxpbek1eq9qeby+gKbS14VCli1k2TdRr9nikMyWyxo/MXHMb/5kaNfrNrKazV3OGsIohgSTr0G0sUxu/Uf5jcPkicouzI5qeWqn0j4J2; 7, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com) 7, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69) 7, 31 -- (envelope-from {samwarren-at-emfocal.com} ) 7, 31 -- id 1Lk8yV-0006fA-BN 7, 31 -- for Microscopy-at-Microscopy.Com; Wed, 18 Mar 2009 21:31:19 -0600 7, 31 -- Received: from 124.192.231.32 ([124.192.231.32]) 7, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com) 7, 31 -- by host361.hostmonster.com with HTTP; 7, 31 -- Wed, 18 Mar 2009 21:31:19 -0600 (MDT) 7, 31 -- Message-ID: {1365.124.192.231.32.1237433479.squirrel-at-host361.hostmonster.com} 7, 31 -- Date: Wed, 18 Mar 2009 21:31:19 -0600 (MDT) 7, 31 -- Subject: TEM - Inviation to a new EM community web site 7, 31 -- From: samwarren-at-emfocal.com 7, 31 -- To: Microscopy-at-Microscopy.Com 7, 31 -- User-Agent: SquirrelMail/1.4.13 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain;charset=iso-8859-1 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-Priority: 3 (Normal) 7, 31 -- Importance: Normal 7, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server} ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 30 -- From StevenLe-at-BaylorHealth.edu Thu Mar 19 08:39:20 2009 22, 30 -- Received: from bhdappagnt02.baylorhealth.edu (mailhost1.bhcs.com [65.248.93.160]) 22, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JDdH5a010099 22, 30 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 08:39:19 -0500 22, 30 -- Received: from BHDAEXIMS01.bhcs.pvt ([10.5.12.120]) 22, 30 -- by bhdappagnt02.baylorhealth.edu (8.14.1/8.14.1) with ESMTP id n2JDdFZ6030680 22, 30 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 08:39:15 -0500 22, 30 -- X-TM-IMSS-Message-ID: {1e54a39e000348d8-at-bhcs.pvt} 22, 30 -- Received: from BHDAEXHT02.bhcs.pvt ([10.5.12.191]) by bhcs.pvt ([10.5.12.120]) with ESMTP (TREND IMSS SMTP Service 7.0; TLS: TLSv1/SSLv3,128bits,RC4-MD5) id 1e54a39e000348d8 for {StevenLe-at-BaylorHealth.edu} ; Thu, 19 Mar 2009 08:39:14 -0600 22, 30 -- Received: from BHDAEXVM32.bhcs.pvt ([10.5.3.122]) by BHDAEXHT02.bhcs.pvt 22, 30 -- ([10.5.12.191]) with mapi; Thu, 19 Mar 2009 08:39:14 -0500 22, 30 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu} 22, 30 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 22, 30 -- Date: Thu, 19 Mar 2009 08:39:13 -0500 22, 30 -- Subject: RE: [Microscopy] TEM - Inviation to a new EM community web site 22, 30 -- Thread-Topic: [Microscopy] TEM - Inviation to a new EM community web site 22, 30 -- Thread-Index: AcmoQ6Cxnf+M1eAUSYKElT/8kHoF2QAU4Qiw 22, 30 -- Message-ID: {492DD4D34D5DC5489E59D73B0506681C0CD82C4AB7-at-BHDAEXVM32.bhcs.pvt} 22, 30 -- References: {200903190334.n2J3YZrD016771-at-ns.microscopy.com} 22, 30 -- In-Reply-To: {200903190334.n2J3YZrD016771-at-ns.microscopy.com} 22, 30 -- Accept-Language: en-US 22, 30 -- Content-Language: en-US 22, 30 -- X-MS-Has-Attach: 22, 30 -- X-MS-TNEF-Correlator: 22, 30 -- acceptlanguage: en-US 22, 30 -- Content-Type: text/plain; charset="iso-8859-1" 22, 30 -- MIME-Version: 1.0 22, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-03-19_09:2009-03-19,2009-03-19,2009-03-19 signatures=0 22, 30 -- Content-Transfer-Encoding: 8bit 22, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2JDdH5a010099 ==============================End of - Headers==============================
For flooring in an EM Room looking for opinions would a static control flooring be benificial? And should it be dissipative or conductive?
I have no experience with eitehr one in an EM Room. Anyone want to vocice an opinion? Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 4, 22 -- From edelmare-at-muohio.edu Thu Mar 19 11:00:34 2009 4, 22 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JG0UWi028370 4, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 19 Mar 2009 11:00:33 -0500 4, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 4, 22 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n2JG0RlX024462 4, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 19 Mar 2009 12:00:27 -0400 4, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 4, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n2JG0OXa021631 4, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 19 Mar 2009 12:00:24 -0400 4, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 4, 22 -- To: microscopy-at-Microscopy.com 4, 22 -- Date: Thu, 19 Mar 2009 12:00:22 -0400 4, 22 -- MIME-Version: 1.0 4, 22 -- Subject: EM room flooring 4, 22 -- Message-ID: {49C233D6.31646.13A14A3E-at-edelmare.muohio.edu} 4, 22 -- Priority: normal 4, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 4, 22 -- Content-type: text/plain; charset=US-ASCII 4, 22 -- Content-transfer-encoding: 7BIT 4, 22 -- Content-description: Mail message body 4, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70 ==============================End of - Headers==============================
we had conductive flooring laid, but it was so long ago that I can't remember the type. I do recall it was the cheaper type because the very best "electronic industry" type wasn't needed.
One thing to be careful about is that if you still handle liquid nitrogen it can crack the vinyl types of floor quite quickly so it's worth having an area reserved for pouring which is not easily damaged.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: edelmare-at-muohio.edu
Just wondering - wouldn't polished concrete, recently suggested by someone as good floor option for LN2 resistance, also be sufficiently dissipative for ESD purposes? Of cause resistivity of such floor will somewhat vary, with time and depending on air humidity etc..., but some references suggest resistance of cured concrete to be around 250 Ohm*M, which is very conductive...
Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Thursday, March 19, 2009 12:35 PM To: vray-at-partbeamsystech.com
Richard
we had conductive flooring laid, but it was so long ago that I can't remember the type. I do recall it was the cheaper type because the very best "electronic industry" type wasn't needed.
One thing to be careful about is that if you still handle liquid nitrogen it can crack the vinyl types of floor quite quickly so it's worth having an area reserved for pouring which is not easily damaged.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: edelmare-at-muohio.edu
Just to confirm- we are very happy with these polished concrete floors (well, they are little unforgiving with dropped glassware) and we have had zero incidents with regard to static buildup which is a problem in Oregon in the winter. Compared to the static problems we had in our old lab with sealed concrete (and indoor carpeting!) we are much happier. john
At 10:37 AM 3/19/2009, vray-at-partbeamsystech.com wrote:
} Just wondering - wouldn't polished concrete, recently suggested by someone } as good floor option for LN2 resistance, also be sufficiently dissipative } for ESD purposes? Of cause resistivity of such floor will somewhat vary, } with time and depending on air humidity etc..., but some references suggest } resistance of cured concrete to be around 250 Ohm*M, which is very } conductive... } } Valery Ray } } ============================ } www.partbeamsystech.com } PBS&T, MEO Engineering Co, Inc. } 290 Broadway St., Suite 298 } Methuen, MA 01844 } Phone: (978) 296-5063 } } } -----Original Message----- } X-from: malcolm.haswell-at-sunderland.ac.uk } [mailto:malcolm.haswell-at-sunderland.ac.uk] } Sent: Thursday, March 19, 2009 12:35 PM } To: vray-at-partbeamsystech.com } Subject: [Microscopy] Re: EM room flooring } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 4, 22 -- From donovan-at-uoregon.edu Thu Mar 19 12:57:30 2009 4, 22 -- Received: from smtp.uoregon.edu (mserv5.uoregon.edu [128.223.142.42]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JHvTTH008264 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 12:57:29 -0500 4, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 4, 22 -- (authenticated bits=0) 4, 22 -- by smtp.uoregon.edu (8.14.3/8.14.3) with ESMTP id n2JHvT6U027068 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 4, 22 -- Thu, 19 Mar 2009 10:57:29 -0700 4, 22 -- Message-Id: {200903191757.n2JHvT6U027068-at-smtp.uoregon.edu} 4, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 4, 22 -- Date: Thu, 19 Mar 2009 10:57:15 -0700 4, 22 -- To: vray-at-partbeamsystech.com 4, 22 -- From: John Donovan {donovan-at-uoregon.edu} 4, 22 -- Subject: Re: [Microscopy] EM room flooring 4, 22 -- Cc: microscopy-at-microscopy.com 4, 22 -- In-Reply-To: {200903191737.n2JHbRRA001449-at-ns.microscopy.com} 4, 22 -- References: {200903191737.n2JHbRRA001449-at-ns.microscopy.com} 4, 22 -- Mime-Version: 1.0 4, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 22 -- X-Virus-Scanned: ClamAV 0.94.2/9141/Thu Mar 19 09:24:25 2009 on mserv5 4, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Many thanks to all who replied to my question about the identity of our critter. Many think it is a virus. Cytomegalovirus, Herpes, HIV, and paramyxavirus were suggested. Others think that it is not a virus, but perhaps yeast, mycoplasma, red blood cells coated with Histogel, or some other unidentified organism. Protein agglomerations, residual bodies, and calcium nodules were also mentioned.
I agree with those who mentioned a uranyl acetate crystal contamination. I had used phosphate buffer, though I did do three ddH2O rinses before en bloc staining. Still, it does look like UAc contamination. On the next round I will switch to cacodylate buffer and also leave out the en bloc staining step to remove all possibility of UAc precipitation. That should clear things up.
I will post the new and improved images from the next round as soon as I have them.
For those of you who might be tuning in late to this discussion, here is the link to the images that we have been talking about.
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School A807 BSRB 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 13, 16 -- From dsoren-at-umich.edu Thu Mar 19 13:16:19 2009 13, 16 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161]) 13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JIGJWE022384 13, 16 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 13:16:19 -0500 13, 16 -- Received: FROM [10.21.131.86] (host-18.subnet-17.med.umich.edu [141.214.17.18]) 13, 16 -- BY tombraider.mr.itd.umich.edu ID 49C28BB7.86E97.5890 ; 13, 16 -- 19 Mar 2009 14:15:19 -0400 13, 16 -- Mime-Version: 1.0 (Apple Message framework v753.1) 13, 16 -- Content-Transfer-Encoding: 7bit 13, 16 -- Message-Id: {44315564-5BB5-4A0E-A1D7-56C3325DC4EB-at-umich.edu} 13, 16 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 13, 16 -- To: microscopy-at-microscopy.com 13, 16 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 13, 16 -- Subject: Critter ID 13, 16 -- Date: Thu, 19 Mar 2009 14:15:18 -0400 13, 16 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Dear all, I'm trying to mount fine-grained coal particles (-125 um) for EDS-based automated mineral analysis. In a first attempt I used epoxy resin (Struers EpoFix), but the BSE intensity of epoxy and coal are too similar, which means the system can't differentiate easily between the two. After doing some background reading I tried mounting the material in molten Carnauba wax. Problem is the polishing is difficult as the wax is extremely soft and the system is taking forever to pump down (I'm not convinced yet it'll ever reach the required vacuum). I'm sure there must be working protocols out there. Does anyone have any recommendations or ideas?
Thanks, Sarah Appleby
-- ********************************* Dr. Sarah Appleby Technical Scientist Advanced Mineralogy Research Center Colorado School of Mines 1310 Maple Street Golden, CO 80401, USA
It looks like calcification or some similar precipitate surrounding the central granular mass. I don't think the roundish particles are regular enough to be viruses. I've seen similar electron-dense material in numerous kidney biopsies which I have always interpreted as calcification within the tissue.
Regards,
John Brealey
Electron Microscope Unit, Surgical Pathology
SA Pathology
Queen Elizabeth Hospital
Woodville, 5011
AUSTRALIA
==============================Original Headers============================== 13, 27 -- From john.brealey-at-imvs.sa.gov.au Thu Mar 19 17:34:27 2009 13, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JMYP6W023471 13, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 17:34:26 -0500 13, 27 -- X-IronPort-AV: E=Sophos;i="4.38,391,1233495000"; 13, 27 -- d="scan'208";a="15802510" 13, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 13, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 20 Mar 2009 09:04:09 +1030 13, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 13, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 13, 27 -- 8.1.263.0; Fri, 20 Mar 2009 09:03:10 +1030 13, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 13, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 142362EC078 for 13, 27 -- {Microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 09:04:09 +1030 (CST) 13, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 13, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 13, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 13, 27 -- Subject: What is this critter? 13, 27 -- Date: Fri, 20 Mar 2009 09:04:08 +1030 13, 27 -- Organization: IMVS 13, 27 -- Message-ID: {000301c9a8e2$d13115a0$7d8a140a-at-41347i} 13, 27 -- MIME-Version: 1.0 13, 27 -- Content-Type: text/plain; charset="us-ascii" 13, 27 -- Content-Transfer-Encoding: 7bit 13, 27 -- X-Mailer: Microsoft Office Outlook 11 13, 27 -- Thread-Index: Acmo4tEKZEE6a2BxTNWZWykLt8BukQ== 13, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
I don't know the answer to your particular problem but in other cases that sound similar to what you are describing, I have found using a polyester mount material works.
Scroll down to the bottom of their list and find their polyester mount material. Part 14675 and 14685 for the smaller size. I don't know of another source but I'm sure they exist.
standard disclaimer: I have no interest in this company except as a satisfied customer...
dj
On Thu, 19 Mar 2009, sappleby-at-mines.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } I'm trying to mount fine-grained coal particles (-125 um) for EDS-based } automated mineral analysis. In a first attempt I used epoxy resin } (Struers EpoFix), but the BSE intensity of epoxy and coal are too } similar, which means the system can't differentiate easily between the } two. After doing some background reading I tried mounting the material } in molten Carnauba wax. Problem is the polishing is difficult as the wax } is extremely soft and the system is taking forever to pump down (I'm not } convinced yet it'll ever reach the required vacuum). I'm sure there must } be working protocols out there. Does anyone have any recommendations or } ideas? } } Thanks, } Sarah Appleby } } -- } ********************************* } Dr. Sarah Appleby } Technical Scientist } Advanced Mineralogy Research Center } Colorado School of Mines } 1310 Maple Street } Golden, CO 80401, USA } } office: 303-384-2487 } fax: 303-384-2499 } email: sappleby-at-mines.edu } } ********************************* } } } } ==============================Original Headers============================== } 7, 16 -- From sappleby-at-mines.edu Thu Mar 19 14:59:33 2009 } 7, 16 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU [138.67.130.5]) } 7, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2JJxXIb006242 } 7, 16 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:59:33 -0500 } 7, 16 -- Received: from [127.0.0.1] (grlmineral03.Mines.EDU [138.67.44.224]) } 7, 16 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id n2JJxWs9006009 } 7, 16 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 13:59:32 -0600 } 7, 16 -- Message-ID: {49C2A424.3040600-at-mines.edu} } 7, 16 -- Date: Thu, 19 Mar 2009 13:59:32 -0600 } 7, 16 -- From: Sarah Appleby {sappleby-at-mines.edu} } 7, 16 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) } 7, 16 -- MIME-Version: 1.0 } 7, 16 -- To: Microscopy-at-Microscopy.Com } 7, 16 -- Subject: fine-grained coal sample prep for SEM? } 7, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 16 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 21 -- From dljones-at-bestweb.net Thu Mar 19 21:29:03 2009 9, 21 -- Received: from mta2.srv.hcvlny.cv.net (mta2.srv.hcvlny.cv.net [167.206.4.197]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2K2T370010501 9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 19 Mar 2009 21:29:03 -0500 9, 21 -- Received: from Pappee (ool-44c57e9d.dyn.optonline.net [68.197.126.157]) 9, 21 -- by mta2.srv.hcvlny.cv.net 9, 21 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 9, 21 -- with ESMTP id {0KGS00GH69KF62X0-at-mta2.srv.hcvlny.cv.net} for 9, 21 -- microscopy-at-microscopy.com; Thu, 19 Mar 2009 22:29:03 -0400 (EDT) 9, 21 -- Date: Thu, 19 Mar 2009 22:29:06 -0400 (Eastern Daylight Time) 9, 21 -- From: "David L. Jones" {dljones-at-bestweb.net} 9, 21 -- Subject: Re: [Microscopy] fine-grained coal sample prep for SEM? 9, 21 -- In-reply-to: {200903192002.n2JK2rCk011513-at-ns.microscopy.com} 9, 21 -- X-X-Sender: dljones-at-imap.bestweb.net 9, 21 -- To: sappleby-at-mines.edu 9, 21 -- Cc: microscopy-at-microscopy.com 9, 21 -- Message-id: {Pine.WNT.4.64.0903192228550.5576-at-Pappee} 9, 21 -- MIME-version: 1.0 9, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 21 -- Content-transfer-encoding: 7BIT 9, 21 -- References: {200903192002.n2JK2rCk011513-at-ns.microscopy.com} ==============================End of - Headers==============================
It seems some people concerned about the legal terms of the site. To tell you the truth, I am total new in starting a community site. I found that term of use which is free to use. So I modified it a little bit and used it. I definitely will write one of my own. All want to say about the terms of use are: (1) Do not abuse the site; (2) I am not responsible on what you post; (3) Copyright belongs to the individual users; (4) I hold the right to modify/delete some contents which may be improper to keep. I may need to add some more. All mostly to cover my ass if there are some legal issues come up. Yes, the terms of use was mostly copied from Harvard website. I just found "Harvard" name was still left in the terms of use. i just removed it. It is not related to Harvard at all. I just borrowed their writing. Believe, my primary reason for setting this up is to serve the community. I have no problem to show my identity. But I am worried showing my real name may turn someone away from using it. I myself would not like to post much on some individual research group site. I keep anonymous just to make you think I am nobody. Don't care about me. This site is for everyone to use. I believe a site making no money will not be long-lived. I want the information posted there to stay for hundreds of years. Think about it, what harm can it do to me if I show myself up? None. It is not a site against any laws. Why would someone want to shut me up? I think this site is straight forward enough. It is for everyone to use. Any individual research group site will not attract world-wide participation. Do you believe a post-doc or a graduate student can keep the site alive long? Do you all want to post on FEI site, JEOL site? The only way to make the site long live is to commercialize it and make it a dedicated community server. I spent most of my recent time on building the site, not on policies. I welcome you comments and wish you can help me out to straighten this site out. I think the functionality of the site is ok. But I am really not good on setting up policies. But I do need legal cover for myself. Again, I sepnt some time on the site structure, but not on the content. I intent to post some of my writting there. I may single-handedly write some TEM, EELS, STEM, FIB, SEM, EDS tutorials. This site may end up mostly just like that. But I encourage your contribution. I have seen many valuable information scattered around list servers, and emails. I just wish they can accumulate at a dedicated place and useful for a long time. I maybe wrong about keeping the site owner anonymous. It can change if most of you demand it. At my work, I helped many people on TEM and other techniques. I felt some of my answers may be written as a FAQ for my colleagues to see. Then I came up an idea about building a TEM-FAQ site. I started searching the ways to build the site and I found out I can do a lot more than a FAQ. And we need more than a FAQ-site. So, after several months, I came up with this: http://www.emfocal.com This site is in fact a personal site. But if this site can gain some money it can live longer and not limited by me as a single person. You see many forum, blog, community sites around. My intention is the same as most of them. Enough said for now. It will be too long for people to read.
Thank you for you attention.
==============================Original Headers============================== 4, 31 -- From samwarren-at-emfocal.com Fri Mar 20 04:16:59 2009 4, 31 -- Received: from outbound-mail-352.bluehost.com (outbound-mail-352.bluehost.com [66.147.249.13]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2K9Gwka000946 4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Mar 2009 04:16:58 -0500 4, 31 -- Received: (qmail 11610 invoked by uid 0); 20 Mar 2009 09:12:13 -0000 4, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161) 4, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 20 Mar 2009 09:12:13 -0000 4, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com; 4, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User; 4, 31 -- b=XNU9zD7exEBRUvI//bp7eRTNLZVIEWnKeStnLA9+GjB3MG4U/BaUv3xAvXIQ9468Xypa9rCpHaEmQGoIakW/RjLZ4wwnzEmv7IwkTZ78A9+WXBqQPWp6W9uOETm+YLcL; 4, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com) 4, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69) 4, 31 -- (envelope-from {samwarren-at-emfocal.com} ) 4, 31 -- id 1LkaqX-0005VN-8B 4, 31 -- for Microscopy-at-Microscopy.Com; Fri, 20 Mar 2009 03:16:58 -0600 4, 31 -- Received: from 124.192.231.2 ([124.192.231.2]) 4, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com) 4, 31 -- by host361.hostmonster.com with HTTP; 4, 31 -- Fri, 20 Mar 2009 03:16:57 -0600 (MDT) 4, 31 -- Message-ID: {1675.124.192.231.2.1237540617.squirrel-at-host361.hostmonster.com} 4, 31 -- Date: Fri, 20 Mar 2009 03:16:57 -0600 (MDT) 4, 31 -- Subject: TEM-reply to Invitation to a new EM community site 4, 31 -- From: samwarren-at-emfocal.com 4, 31 -- To: Microscopy-at-Microscopy.Com 4, 31 -- User-Agent: SquirrelMail/1.4.13 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain;charset=iso-8859-1 4, 31 -- Content-Transfer-Encoding: 8bit 4, 31 -- X-Priority: 3 (Normal) 4, 31 -- Importance: Normal 4, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server} ==============================End of - Headers==============================
we had the conductive floor in the microscope room and wooden floor where the liquid nitrogen was stored so it seemed a good arrangement. The conductive floor was easy to keep clean, too.
We certainly never saw any problems with static.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
----- Original Message ----- X-from: "Richard E. Edelmann" {edelmare-at-muohio.edu}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mike.net.mo-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mike.net.mo-at-gmail.com Name: Xmfan
Organization: USTC
Title-Subject: [Filtered] Change the contrast between the outer and inner of the diffraction circle to a linear value
Question: In the experiment we imaged a silica bead（2um） , and we gained the image of a silica bead which was a diffraction ring, and the brightness of the diffraction ring changed with the distance. （darker in the circle and lower outside the circle）. Now we want to change the contrast between the outer and inner of the diffraction circle to a linear value so that I can know the positon of the bead, I don't know which lens or optical parts can be used to achieve this function.
==============================Original Headers============================== 6, 12 -- From zaluzec-at-microscopy.com Fri Mar 20 07:01:16 2009 6, 12 -- Received: from [192.168.1.101] (msdvpn072.msd.anl.gov [130.202.238.72]) 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KC1Dg6000889 6, 12 -- for {microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 07:01:15 -0500 6, 12 -- Mime-Version: 1.0 6, 12 -- Message-Id: {p06240800c5e935ee36c5-at-[10.0.239.114]} 6, 12 -- Date: Fri, 20 Mar 2009 07:01:13 -0500 6, 12 -- To: microscopy-at-microscopy.com 6, 12 -- From: mike.net.mo-at-gmail.com (by way of MicroscopyListserver) 6, 12 -- Subject: viaWWW: Change the contrast between the outer and inner of the 6, 12 -- diffraction circle to a linear value 6, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
One of my sons favourite Back Street Boys songs has the line "I don't care just who you are, What you've done...."
Unfortunately that does not apply in science. When you do a bad job of plagiarizing another site, and admit it, and say that you want to remain anonymous for any reason you destroy any credibility.
Paul Hazelton, not anonymous
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 6, 20 -- From paul_hazelton-at-umanitoba.ca Fri Mar 20 07:43:39 2009 6, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KChdjU015704 6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 07:43:39 -0500 6, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 6, 20 -- (authenticated bits=0) 6, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n2KChbUv012255; 6, 20 -- Fri, 20 Mar 2009 07:43:37 -0500 (CDT) 6, 20 -- Message-ID: {49C38F7B.60602-at-umanitoba.ca} 6, 20 -- Date: Fri, 20 Mar 2009 07:43:39 -0500 6, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 20 -- User-Agent: Thunderbird 2.0.0.19 (Windows/20081209) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: samwarren-at-emfocal.com 6, 20 -- Subject: Re: [Microscopy] TEM-reply to Invitation to a new EM community site 6, 20 -- References: {200903200919.n2K9Jb1u004043-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200903200919.n2K9Jb1u004043-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
This is to inform you and invite any interested participants to the 3rd Advanced Materials Characterization Workshop 2009, organized by the Center for Microanalysis of Materials, Frederick Seitz Materials Research Laboratory, Univeristy of Illinois.
Dates: June 3 and 4, 2009
More information at: http://cmm.mrl.uiuc.edu/workshop2009/ {http://cmm.mrl.uiuc.edu/workshop2009/}
Kind regards
Ivan Petrov
217 333 8396
p.s. The workshop will provide a condensed overview of the most important analytical techniques with strong focus in practical applications and problem solving strategies. The lectures will cover mainstream techniques such as:
atomic force microscopy (AFM)
x-ray diffraction, reflectivity and fluorescence (XRD, XRR and XRF)
scanning electron microscopy (SEM)
focused ion beam (FIB)
Auger electron spectroscopy (AES)
x-ray photoelectron spectroscopy (XPS)
transmission and scanning transmission electron microscopy (TEM, STEM)
secondary ion mass spectrometry (SIMS)
Rutherford backscattering (RBS)
optical spectroscopy (Raman, Photoluminescence, FTIR, ellipsometry), etc.
The lectures will be given by FS-MRL facility scientists with 10+ years of hands-on experience in each particular technique. The workshop will cover:
The fundamentals of each analytical technique.
Comparative review of the instrumentation options with emphasis on differences in resolution, sensitivity, sample requirements.
Data acquisition strategies and data interpretation methods.
Expert tips on how to avoid measurement artifacts.
Critical review of strengths and weaknesses of each technique: how to combine techniques to extract the best possible complementary information.
Detailed discussion of practical examples including industrial applications in nanotechnology, microelectronics, thin films, coatings, bioengineering, mineralogy, medical and pharmaceutical research.
Instrument vendors and industrial scientists will be present during the workshop to discuss new instrumentation, applications and technology. Laboratory tours and demonstration of the main instruments available at the CMM will be given during the workshop.
Registration is required.
Register now - space is limited.
Join the sponsors:
Asylum Research
AVS - University of Illinois at Urbana-Champaign Student Chapter
Bruker AXS
Chemplex
FEI
Gatan
Materials Data
PANalytical
Physical Electronics (PHI)
==============================Original Headers============================== 48, 20 -- From petrov-at-mrl.uiuc.edu Fri Mar 20 11:32:12 2009 48, 20 -- Received: from mrlnt6.mrl.uiuc.edu (mrlnt6.mrl.uiuc.edu [130.126.101.9]) 48, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KGWBLq004314 48, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 11:32:12 -0500 48, 20 -- Content-Class: urn:content-classes:message 48, 20 -- MIME-Version: 1.0 48, 20 -- Content-Type: text/plain; 48, 20 -- charset="utf-8" 48, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6619.12 48, 20 -- Subject: 3rd Materials Characterization Workshop, Center for Microanalysis of Materials, Univeristy of Illinois 48, 20 -- Date: Fri, 20 Mar 2009 11:32:09 -0500 48, 20 -- Message-ID: {9EADC1E53F9C70479BF6559370369114011E7034-at-mrlnt6.mrl.uiuc.edu} 48, 20 -- X-MS-Has-Attach: 48, 20 -- X-MS-TNEF-Correlator: 48, 20 -- Thread-Topic: 3rd Materials Characterization Workshop, Center for Microanalysis of Materials, Univeristy of Illinois 48, 20 -- Thread-Index: AcmpeWpl2aNdy6v2QymB4V99Z1/x8Q== 48, 20 -- From: "Ivan Petrov" {petrov-at-mrl.uiuc.edu} 48, 20 -- To: {Microscopy-at-microscopy.com} 48, 20 -- Content-Transfer-Encoding: 8bit 48, 20 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2KGWBLq004314 ==============================End of - Headers==============================
The EM Facility in the Department of Materials, University of Oxford, has an opening for an Electron Microscope Technician. The job involves the maintenance and repair of the instruments, the basic instruction of users in the operation of the instruments and the day to day running of the facility. The closing date for applications is Tuesday 14 April 2009. For details please see http://www.materials.ox.ac.uk/vacancies/
Thanks! Dr Crispin Hetherington Department of Materials University of Oxford, UK www-em.materials.ox.ac.uk
==============================Original Headers============================== 6, 26 -- From crispin.hetherington-at-materials.ox.ac.uk Fri Mar 20 11:36:12 2009 6, 26 -- Received: from relay0.mail.ox.ac.uk (relay0.mail.ox.ac.uk [129.67.1.161]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KGaBYF007464 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 11:36:12 -0500 6, 26 -- Received: from smtp0.mail.ox.ac.uk ([129.67.1.205]) 6, 26 -- by relay0.mail.ox.ac.uk with esmtp (Exim 4.69) 6, 26 -- (envelope-from {crispin.hetherington-at-materials.ox.ac.uk} ) 6, 26 -- id 1LkhhZ-0005Cm-36 6, 26 -- for Microscopy-at-microscopy.com; Fri, 20 Mar 2009 16:36:09 +0000 6, 26 -- Received: from crispin-macmini.materials.ox.ac.uk ([129.67.84.177]) 6, 26 -- by smtp0.mail.ox.ac.uk with esmtpsa (TLSv1:AES128-SHA:128) 6, 26 -- (Exim 4.69) 6, 26 -- (envelope-from {crispin.hetherington-at-materials.ox.ac.uk} ) 6, 26 -- id 1LkhhZ-0003pQ-2M 6, 26 -- for Microscopy-at-microscopy.com; Fri, 20 Mar 2009 16:36:09 +0000 6, 26 -- Message-Id: {8649EDB7-9084-4191-803E-16FC41BE4A2B-at-materials.ox.ac.uk} 6, 26 -- From: Crispin Hetherington {crispin.hetherington-at-materials.ox.ac.uk} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 26 -- Subject: Job opening, EM Technician 6, 26 -- Date: Fri, 20 Mar 2009 16:36:08 +0000 6, 26 -- References: {B7BF4B3D-E6EB-492A-8ED5-6F355CF18769-at-materials.ox.ac.uk} 6, 26 -- X-Mailer: Apple Mail (2.930.3) 6, 26 -- X-Oxford-Username: crispin ==============================End of - Headers==============================
On Mar 20, 2009, at 5:01 AM, mike.net.mo-at-gmail.com wrote:
} Title-Subject: [Filtered] Change the contrast between the outer and } inner of the diffraction circle to a linear value } } Question: In the experiment we imaged a silica } bead（2um） , and we gained the image of a silica bead } which was a diffraction ring, and the brightness of the diffraction } ring changed with the distance. （darker in the circle and } lower outside the circle）. Now we want to change the contrast } between the outer and inner of the diffraction circle to a linear } value so that I can know the positon of the bead, I don't know which } lens or optical parts can be used to achieve this function. } Dear Mike, Since no one else has tackled this, I'll give it a try. You don't say whether you are doing EM or LM, but the answer is much the same. In either case, you have phase contrast, which depends on the finite wavelength of the particles used for imaging. In the case of TEM, the width of the fringes depends on the amount of defocus, so if you take a series of images with varying focus values--a through-focus series-- you can make the width of the fringes get smaller, then larger, and you will get a sign reversal when going through focus. What you describe above would be an overfocus fringe in TEM, if you are describing a CCD image. (It would be underfocus, if you are describing a photographic negative.) In the LM, it all depends on the imaging conditions. For some techniques, a phase plate is introduced into the illumination pathway, so in that case, the width of the fringes can be changed by adjusting the position of the phase plate. Warning: I have not done any LM, except on rare occasion to see if cells I was culturing looked like they were growing properly, so I'm just guessing on what can be adjusted or how to do it. If you describe what you are doing more completely, perhaps a real expert would be able to give you better advice. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Mar 20 18:40:20 2009 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2KNeJWI011759 4, 22 -- for {microscopy-at-microscopy.com} ; Fri, 20 Mar 2009 18:40:19 -0500 4, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 4, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id EB44D2E50716; 4, 22 -- Fri, 20 Mar 2009 16:40:17 -0700 (PDT) 4, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 4, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 4, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 691DB2E503DB; 4, 22 -- Fri, 20 Mar 2009 16:40:16 -0700 (PDT) 4, 22 -- Message-Id: {9899E8A7-9440-45A3-AAAD-4F584FDBB0A1-at-caltech.edu} 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- To: mike.net.mo-at-gmail.com, microscopy-at-microscopy.com 4, 22 -- In-Reply-To: {200903201201.n2KC1RkL001032-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 4, 22 -- Subject: Re: [Microscopy] viaWWW: Change the contrast between the outer and inner of the 4, 22 -- Date: Fri, 20 Mar 2009 16:40:16 -0700 4, 22 -- References: {200903201201.n2KC1RkL001032-at-ns.microscopy.com} 4, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
From serenajones300-at-netscape.net Sat Mar 21 09:54:15 2009 Return-Path: {serenajones300-at-netscape.net} Received: from google.com (cpc4-brig10-0-0-cust702.brig.cable.ntl.com [81.108.230.191]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2LEsEI6014487 for {microscopylistserverarchive-at-microscopy.com} ; Sat, 21 Mar 2009 09:54:15 -0500 Received: from [102.149.75.174] (HELO google.com) by tall-rap.co.uk; Sat, 21 Mar 2009 14:54:13 +0000 Message-ID: {00000001EBD322F957528816} Reply-To: Dinah Elless {1298gretzinger.gunnar-at-gmail.com}
Dear All
My laboratory is participating in an electron microscopy length calibration exercise to understand the precision, accuracy and long-term reliability of distance measurements made with TEM.
Q1. I am aware that NIST have a standard procedure for calibrating SEMs and carrying out measurements, but am I correct is saying there is no equivalent for TEM - either from NIST or any other agency?
Q2. Am I also correct in believing there are no accredited distance calibration standards for TEM ie a certified standard produced by NIST or similar? I am aware of MagiCal, diffraction gratings and crystal lattices as references, but none of these are certified standards - note standards and references are often confused, but are not the same thing.
Q3. If anyone has carried out a similar exercise to this and has published the results, I'd be interested in any references.
Thanks and regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
==============================Original Headers============================== 12, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 22 17:13:25 2009 12, 24 -- Received: from kabul.ucc.usyd.edu.au (kabul.ucc.usyd.edu.au [129.78.220.1]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2MMDOvC007699 12, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 17:13:24 -0500 12, 24 -- Received: from kabul.ucc.usyd.edu.au (localhost [127.0.0.1]) 12, 24 -- by localhost (Postfix) with SMTP id 2D6CE5FDC2 12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST) 12, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19]) 12, 24 -- by kabul.ucc.usyd.edu.au (Postfix) with ESMTP id 15CDB5FDB5 12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST) 12, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server HTTP-DAV ; 12, 24 -- Sun, 22 Mar 2009 22:13:21 +0000 12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 24 -- Date: Mon, 23 Mar 2009 09:13:20 +1100 12, 24 -- Subject: RE: TEM length calibration standards 12, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au} 12, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 24 -- Message-ID: {C5ED0330.597%david.mitchell-at-emu.usyd.edu.au} 12, 24 -- Thread-Topic: TEM length calibration standards 12, 24 -- Thread-Index: AcmrO2hqpzsbABcuEd6gAAAjMrpsqg== 12, 24 -- Mime-version: 1.0 12, 24 -- Content-type: text/plain; 12, 24 -- charset="US-ASCII" 12, 24 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
I just want to have a frank and honest talk with you. It seems that my decision of keeping anonymous was a wrong decision at the start. I will put my name on it later (after several months testing). I still think this is the initial testing stage (usage, politics, user feed back, etc.)
I don't know what I have done wrong, besides being anonymous, to cause some of you "hate" this site. I have all the good intention to start this site. I am a researcher just like many of you here. I have been working in the field of TEM for almost 20 yearsC and loving itB
No rush on using the site. Just want you guys know it is there. I am really hopping some of you may end up helping me moderate the forum, wiki books, faqsC image galleries on the site. I used the term gadministratorsh on the site, by that I meant some of you in the future. I can have some people helping me administrating the site. I will simplify the gterms of usehB I did not start this site as a personB Mainly because to separate myself from any possible legal issue with the siteB I cannot start the site without some legal claimsB Some of the terms may sound like offensiveB I am sorry about thatBAgainC no experience writing that stuffC and I donet want to miss some critical points so that someone can sue meB AgainC I broadcast the site here for announcementC and also soliciting your inputB
RegardsC Sam
} You "site" is doomed to fail, amongst the users } of this MSA listserver. If only for your poor } communication skills. } } sorry, } } Jim Quinn } } } } } } } From mail-at-ns.microscopy.com Fri Mar 20 05:17:21 2009 } } Return-Path: {mail-at-ns.microscopy.com} } } Received: from ns.microscopy.com (microscopy.com [206.69.208.10]) } } by www.matscieng.sunysb.edu (8.11.6/8.11.6) with ESMTP id n2KAHJM28179 } } for {jquinn-at-www.matscieng.sunysb.edu} ; Fri, 20 Mar 2009 05:17:20 -0500 } } Received: from ns.microscopy.com (localhost.localdomain [127.0.0.1]) } } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n2K9HvRv002063 } } for {jquinn-at-www.matscieng.sunysb.edu} ; Fri, 20 Mar 2009 04:17:57 -0500 } } Received: (from mail-at-localhost) } } by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id } n2K9Hvb2002060; } } Fri, 20 Mar 2009 04:17:57 -0500 } } Date: Fri, 20 Mar 2009 04:17:57 -0500 } } Message-Id: {200903200917.n2K9Hvb2002060-at-ns.microscopy.com} } } To: jquinn-at-www.matscieng.sunysb.edu } } From: samwarren-at-emfocal.com } } Reply-to: samwarren-at-emfocal.com } } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } } Subject: [Microscopy] TEM-reply to Invitation to a new EM community } site } } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } X-lewp: MicroscopyListSpam NAGS } } Status: R } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } It seems some people concerned about the legal terms of the site. To } tell } } you the truth, I am total new in starting a community site. I found } that } } term of use which is free to use. So I modified it a little bit and } used } } it. I definitely will write one of my own. All want to say about the } terms } } of use are: (1) Do not abuse the site; (2) I am not responsible on what } } you post; (3) Copyright belongs to the individual users; (4) I hold the } } right to modify/delete some contents which may be improper to keep. } } I may need to add some more. All mostly to cover my ass if there are } some } } legal issues come up. } } Yes, the terms of use was mostly copied from Harvard website. I just } found } } "Harvard" name was still left in the terms of use. i just removed it. } It } } is not related to Harvard at all. I just borrowed their writing. } } Believe, my primary reason for setting this up is to serve the } community. } } I have no problem to show my identity. But I am worried showing my real } } name may turn someone away from using it. I myself would not like to } post } } much on some individual research group site. I keep anonymous just to } make } } you think I am nobody. Don't care about me. This site is for everyone } to } } use. I believe a site making no money will not be long-lived. I want } the } } information posted there to stay for hundreds of years. } } Think about it, what harm can it do to me if I show myself up? None. It } is } } not a site against any laws. Why would someone want to shut me up? I } think } } this site is straight forward enough. It is for everyone to use. Any } } individual research group site will not attract world-wide } participation. } } Do you believe a post-doc or a graduate student can keep the site alive } } long? Do you all want to post on FEI site, JEOL site? The only way to } make } } the site long live is to commercialize it and make it a dedicated } } community server. } } I spent most of my recent time on building the site, not on policies. I } } welcome you comments and wish you can help me out to straighten this } site } } out. I think the functionality of the site is ok. But I am really not } good } } on setting up policies. But I do need legal cover for myself. } } Again, I sepnt some time on the site structure, but not on the content. } I } } intent to post some of my writting there. I may single-handedly write } some } } TEM, EELS, STEM, FIB, SEM, EDS tutorials. This site may end up mostly } just } } like that. But I encourage your contribution. I have seen many valuable } } information scattered around list servers, and emails. I just wish they } } can accumulate at a dedicated place and useful for a long time. } } I maybe wrong about keeping the site owner anonymous. It can change if } } most of you demand it. } } At my work, I helped many people on TEM and other techniques. I felt } some } } of my answers may be written as a FAQ for my colleagues to see. Then I } } came up an idea about building a TEM-FAQ site. I started searching the } } ways to build the site and I found out I can do a lot more than a FAQ. } And } } we need more than a FAQ-site. So, after several months, I came up with } } this: } } http://www.emfocal.com } } This site is in fact a personal site. But if this site can gain some } money } } it can live longer and not limited by me as a single person. You see } many } } forum, blog, community sites around. My intention is the same as most } of } } them. } } Enough said for now. It will be too long for people to read. } } } } Thank you for you attention. } } } } } } } } ==============================Original } Headers============================== } } 4, 31 -- From samwarren-at-emfocal.com Fri Mar 20 04:16:59 2009 } } 4, 31 -- Received: from outbound-mail-352.bluehost.com } (outbound-mail-352.bluehost.com [66.147.249.13]) } } 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } n2K9Gwka000946 } } 4, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Mar 2009 04:16:58 } -0500 } } 4, 31 -- Received: (qmail 11610 invoked by uid 0); 20 Mar 2009 09:12:13 } -0000 } } 4, 31 -- Received: from unknown (HELO host361.hostmonster.com) } (66.147.240.161) } } 4, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 20 } Mar 2009 09:12:13 -0000 } } 4, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; } d=emfocal.com; } } 4, 31 -- } h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User; } } 4, 31 -- } b=XNU9zD7exEBRUvI//bp7eRTNLZVIEWnKeStnLA9+GjB3MG4U/BaUv3xAvXIQ9468Xypa9rCpHaEmQGoIakW/RjLZ4wwnzEmv7IwkTZ78A9+WXBqQPWp6W9uOETm+YLcL; } } 4, 31 -- Received: from localhost ([127.0.0.1] } helo=host361.hostmonster.com) } } 4, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69) } } 4, 31 -- (envelope-from {samwarren-at-emfocal.com} ) } } 4, 31 -- id 1LkaqX-0005VN-8B } } 4, 31 -- for Microscopy-at-Microscopy.Com; Fri, 20 Mar 2009 03:16:58 } -0600 } } 4, 31 -- Received: from 124.192.231.2 ([124.192.231.2]) } } 4, 31 -- (SquirrelMail authenticated user } samwarren-at-emfocal.com) } } 4, 31 -- by host361.hostmonster.com with HTTP; } } 4, 31 -- Fri, 20 Mar 2009 03:16:57 -0600 (MDT) } } 4, 31 -- Message-ID: } {1675.124.192.231.2.1237540617.squirrel-at-host361.hostmonster.com} } } 4, 31 -- Date: Fri, 20 Mar 2009 03:16:57 -0600 (MDT) } } 4, 31 -- Subject: TEM-reply to Invitation to a new EM community site } } 4, 31 -- From: samwarren-at-emfocal.com } } 4, 31 -- To: Microscopy-at-Microscopy.Com } } 4, 31 -- User-Agent: SquirrelMail/1.4.13 } } 4, 31 -- MIME-Version: 1.0 } } 4, 31 -- Content-Type: text/plain;charset=iso-8859-1 } } 4, 31 -- Content-Transfer-Encoding: 8bit } } 4, 31 -- X-Priority: 3 (Normal) } } 4, 31 -- Importance: Normal } } 4, 31 -- X-Identified-User: } {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program } running on server} } } ==============================End of - } Headers============================== } } }
==============================Original Headers============================== 7, 33 -- From samwarren-at-emfocal.com Sun Mar 22 21:22:49 2009 7, 33 -- Received: from outbound-mail-345.bluehost.com (outbound-mail-345.bluehost.com [66.147.249.6]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2N2Mlqm025968 7, 33 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Mar 2009 21:22:48 -0500 7, 33 -- Received: (qmail 12896 invoked by uid 0); 23 Mar 2009 02:17:54 -0000 7, 33 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161) 7, 33 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 23 Mar 2009 02:17:54 -0000 7, 33 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com; 7, 33 -- h=Message-ID:In-Reply-To:References:Date:Subject:From:To:Cc:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User; 7, 33 -- b=tSH6R0ikF86A8PYCAkH7YcI1F9IHf16WuhpC962tjU/+f+cjvNCgutvLZD/gkyd9/VjSvZZk2BGLVSaSR1V89JcZke41Tv14BN5TcfFlIFo/31AExQhXC+Hk9DO/4G9N; 7, 33 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com) 7, 33 -- by host361.hostmonster.com with esmtpa (Exim 4.69) 7, 33 -- (envelope-from {samwarren-at-emfocal.com} ) 7, 33 -- id 1LlZoL-0006Wy-0m; Sun, 22 Mar 2009 20:22:45 -0600 7, 33 -- Received: from 124.192.231.7 ([124.192.231.7]) 7, 33 -- (SquirrelMail authenticated user samwarren-at-emfocal.com) 7, 33 -- by host361.hostmonster.com with HTTP; 7, 33 -- Sun, 22 Mar 2009 20:22:45 -0600 (MDT) 7, 33 -- Message-ID: {1544.124.192.231.7.1237774965.squirrel-at-host361.hostmonster.com} 7, 33 -- In-Reply-To: {200903201720.n2KHKhx28887-at-www.matscieng.sunysb.edu} 7, 33 -- References: {200903201720.n2KHKhx28887-at-www.matscieng.sunysb.edu} 7, 33 -- Date: Sun, 22 Mar 2009 20:22:45 -0600 (MDT) 7, 33 -- Subject: Re: [Microscopy] TEM-reply to Invitation to a new EM community site 7, 33 -- From: samwarren-at-emfocal.com 7, 33 -- To: "Jim Quinn" {jquinn-at-www.matscieng.sunysb.edu} 7, 33 -- Cc: Microscopy-at-Microscopy.Com 7, 33 -- User-Agent: SquirrelMail/1.4.13 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Type: text/plain;charset=iso-8859-1 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-Priority: 3 (Normal) 7, 33 -- Importance: Normal 7, 33 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server} ==============================End of - Headers==============================
We make several traceable and non-traceable calibration standards for SEM and AFM, now including a 70-nm pitch grating. What procedure would one use to make a TEM specimen from a grating? If the procedure is to cast and then peel a polymer replica, can this be done without significant distortion of the length?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: david.mitchell-at-emu.usyd.edu.au To: donc-at-asmicro.com Sent: Sunday, March 22, 2009 6:18 PM Subject: [a] [Microscopy] RE: TEM length calibration standards
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
Dear All
My laboratory is participating in an electron microscopy length calibration exercise to understand the precision, accuracy and long-term reliability of distance measurements made with TEM.
Q1. I am aware that NIST have a standard procedure for calibrating SEMs and carrying out measurements, but am I correct is saying there is no equivalent for TEM - either from NIST or any other agency?
Q2. Am I also correct in believing there are no accredited distance calibration standards for TEM ie a certified standard produced by NIST or similar? I am aware of MagiCal, diffraction gratings and crystal lattices as references, but none of these are certified standards - note standards and references are often confused, but are not the same thing.
Q3. If anyone has carried out a similar exercise to this and has published the results, I'd be interested in any references.
Thanks and regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
==============================Original Headers============================== 12, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 22 17:13:25 2009 12, 24 -- Received: from kabul.ucc.usyd.edu.au (kabul.ucc.usyd.edu.au [129.78.220.1]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2MMDOvC007699 12, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 17:13:24 -0500 12, 24 -- Received: from kabul.ucc.usyd.edu.au (localhost [127.0.0.1]) 12, 24 -- by localhost (Postfix) with SMTP id 2D6CE5FDC2 12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST) 12, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19]) 12, 24 -- by kabul.ucc.usyd.edu.au (Postfix) with ESMTP id 15CDB5FDB5 12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST) 12, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server HTTP-DAV ; 12, 24 -- Sun, 22 Mar 2009 22:13:21 +0000 12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 24 -- Date: Mon, 23 Mar 2009 09:13:20 +1100 12, 24 -- Subject: RE: TEM length calibration standards 12, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au} 12, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 24 -- Message-ID: {C5ED0330.597%david.mitchell-at-emu.usyd.edu.au} 12, 24 -- Thread-Topic: TEM length calibration standards 12, 24 -- Thread-Index: AcmrO2hqpzsbABcuEd6gAAAjMrpsqg== 12, 24 -- Mime-version: 1.0 12, 24 -- Content-type: text/plain; 12, 24 -- charset="US-ASCII" 12, 24 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 26 -- From donc-at-asmicro.com Sun Mar 22 22:35:56 2009 21, 26 -- Received: from smtp121.sbc.mail.re3.yahoo.com (smtp121.sbc.mail.re3.yahoo.com [66.196.96.94]) 21, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2N3ZtRJ008785 21, 26 -- for {microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 22:35:55 -0500 21, 26 -- Received: (qmail 69341 invoked from network); 23 Mar 2009 03:35:53 -0000 21, 26 -- Received: from unknown (HELO asm15) (donc-at-98.226.80.44 with login) 21, 26 -- by smtp121.sbc.mail.re3.yahoo.com with SMTP; 23 Mar 2009 03:35:53 -0000 21, 26 -- X-YMail-OSG: P1j19lEVM1lKYCfXkN6Y_ykVgUS2Iu6Qx8P.hI0133lfsxxVHmvoopNJeJjCKNGT9v1uR0jXh1Jx4KK3NehHOM0tMqR0MSVnFNsbimK0qNI7ajbGeGgszeqcVw4RZQQapezMVR0qThFjZuvixm4H1OQhJh.CBVKImezStfkW4Jj5OQiO2scIg2ru1MU1XqxJ83is9B8FR3D4UCzIsNX0BHISrfIHpeU- 21, 26 -- X-Yahoo-Newman-Property: ymail-3 21, 26 -- Message-ID: {88BC6672C7E5479995672EAE7DF80ACE-at-asm15} 21, 26 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 21, 26 -- To: {david.mitchell-at-emu.usyd.edu.au} 21, 26 -- Cc: "Microscopy List" {microscopy-at-microscopy.com} 21, 26 -- References: {200903222218.n2MMIw9P012191-at-ns.microscopy.com} 21, 26 -- Subject: Re: [a] [Microscopy] RE: TEM length calibration standards 21, 26 -- Date: Sun, 22 Mar 2009 23:12:46 -0400 21, 26 -- MIME-Version: 1.0 21, 26 -- Content-Type: text/plain; 21, 26 -- format=flowed; 21, 26 -- charset="iso-8859-1"; 21, 26 -- reply-type=original 21, 26 -- Content-Transfer-Encoding: 7bit 21, 26 -- X-Priority: 3 21, 26 -- X-MSMail-Priority: Normal 21, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 21, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
I independently replied to Sarah last week from home. I am just now replying to the list as my web-based mail client won't let me send plain text messages, so they get bounced.
I followed another's suggestion to dissolve iodoform in epoxy to boost the average atomic number of the epoxy without interfering with polymerization. It is a somewhat nasty material, so it needs to be handled with care. However, it definitely did the job for me for my research on minerals in coal in the 1980s and 1990s.
I am curious how the polyester resin would offer an improvement.
Most polymers are quite similar in atomic number. I tried many in my research to find a contrasting medium. Something like carnauba wax or polyethylene or polystyrene have a higher H:C ratio than coal and without many other functional groups that serve to raise the average atomic number. However, wax and polyethylene should be the extreme cases, yet the contrast was not all that satisfactory. Besides, as thermoplastics, the preparation of mounts was somewhat problematic. Raising the atomic number by the addition of a heavy element seemed a better alternative.
Warren S.
-----Original Message----- X-from: dljones-at-bestweb.net [mailto:dljones-at-bestweb.net] Sent: Thursday, March 19, 2009 9:31 PM To: wesaia-at-iastate.edu
Sarah,
I don't know the answer to your particular problem but in other cases that sound similar to what you are describing, I have found using a polyester
Scroll down to the bottom of their list and find their polyester mount material. Part 14675 and 14685 for the smaller size. I don't know of another source but I'm sure they exist.
standard disclaimer: I have no interest in this company except as a satisfied customer...
dj
On Thu, 19 Mar 2009, sappleby-at-mines.edu wrote:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } Dear all, } I'm trying to mount fine-grained coal particles (-125 um) for EDS-based } automated mineral analysis. In a first attempt I used epoxy resin } (Struers EpoFix), but the BSE intensity of epoxy and coal are too } similar, which means the system can't differentiate easily between the } two. After doing some background reading I tried mounting the material } in molten Carnauba wax. Problem is the polishing is difficult as the wax } is extremely soft and the system is taking forever to pump down (I'm not } convinced yet it'll ever reach the required vacuum). I'm sure there must } be working protocols out there. Does anyone have any recommendations or } ideas? } } Thanks, } Sarah Appleby } } -- } ********************************* } Dr. Sarah Appleby } Technical Scientist } Advanced Mineralogy Research Center } Colorado School of Mines } 1310 Maple Street } Golden, CO 80401, USA } } office: 303-384-2487 } fax: 303-384-2499 } email: sappleby-at-mines.edu } } *********************************
==============================Original Headers============================== 18, 36 -- From wesaia-at-iastate.edu Mon Mar 23 12:04:10 2009 18, 36 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 18, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2NH4A1l011189 18, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 12:04:10 -0500 18, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 18, 36 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n2NH49Qo030374 18, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 12:04:09 -0500 18, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp 18, 36 -- id 5ed8_a02c75a4_17cc_11de_9e53_001372578af6; 18, 36 -- Mon, 23 Mar 2009 12:04:09 -0500 18, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 18, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n2NH44oc029812 18, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 12:04:07 -0500 18, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 18, 36 -- Mon, 23 Mar 2009 11:51:25 -0500 18, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 36 -- Content-class: urn:content-classes:message 18, 36 -- MIME-Version: 1.0 18, 36 -- Content-Type: text/plain; 18, 36 -- charset="us-ascii" 18, 36 -- Subject: RE: [Microscopy] Re: fine-grained coal sample prep for SEM? 18, 36 -- Date: Mon, 23 Mar 2009 11:51:41 -0500 18, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703B904E0-at-maire.eng.iastate.edu} 18, 36 -- In-Reply-To: {200903200230.n2K2UgUX011661-at-ns.microscopy.com} 18, 36 -- X-MS-Has-Attach: 18, 36 -- X-MS-TNEF-Correlator: 18, 36 -- Thread-Topic: [Microscopy] Re: fine-grained coal sample prep for SEM? 18, 36 -- thread-index: AcmpA99ULhFfW+21Q/GltFXfoxFoPwC0jofQ 18, 36 -- References: {200903200230.n2K2UgUX011661-at-ns.microscopy.com} 18, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 18, 36 -- To: {Microscopy-at-microscopy.com} 18, 36 -- X-OriginalArrivalTime: 23 Mar 2009 16:51:25.0520 (UTC) FILETIME=[9A807D00:01C9ABD7] 18, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.23.164917 18, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 18, 36 -- Content-Transfer-Encoding: 8bit 18, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2NH4A1l011189 ==============================End of - Headers==============================
I have a client who wants to do cryo immuno EM of cells grown on culture insert membranes. I notice that the membranes can be of various materials such as PTFE or polycarbonate. Does anyone have experience cryo sectioning insert membranes and if so which type of membrane sections well?
Thank you in advance.
Bob Temkin Advanced Bioimaging Centre Mount Sinai Hospital Pathology and Laboratory Medicine Toronto
rtemkin-at-mtsinai.on.ca
==============================Original Headers============================== 6, 21 -- From RTemkin-at-mtsinai.on.ca Mon Mar 23 14:32:48 2009 6, 21 -- Received: from mshemr01.mtsinai.on.ca (mshemr01.mtsinai.on.ca [38.112.100.7]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2NJWlot030109 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 14:32:47 -0500 6, 21 -- Received: from [142.223.135.154] ([142.223.135.154]:48139 "EHLO 6, 21 -- mshmail3.mtsinai.on.ca") by mshemr01.mtsinai.on.ca with ESMTP 6, 21 -- id S6873964AbZCWTcr (ORCPT {rfc822;Microscopy-at-microscopy.com} ); 6, 21 -- Mon, 23 Mar 2009 15:32:47 -0400 6, 21 -- Received: by MSHMAIL3 with Internet Mail Service (5.5.2657.72) 6, 21 -- id {HJ1H3NQS} ; Mon, 23 Mar 2009 15:32:47 -0400 6, 21 -- Message-ID: {462EBF7807AE844E9AE4E0E70AF52C221EDDDD-at-mshmail12} 6, 21 -- From: "Temkin, Robert" {RTemkin-at-mtsinai.on.ca} 6, 21 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 21 -- Subject: Cryo immuno EM of culture inserts 6, 21 -- Date: Mon, 23 Mar 2009 15:32:45 -0400 6, 21 -- MIME-Version: 1.0 6, 21 -- X-Mailer: Internet Mail Service (5.5.2657.72) 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="iso-8859-1" 6, 21 -- X-BCN: Meridius 1000 Version 3.4 on mshemr01.mtsinai.on.ca 6, 21 -- X-BCN-Sender: RTemkin-at-mtsinai.on.ca ==============================End of - Headers==============================
David, Since the Mag-i-cal sample traces all of the different lengths scales back to the d-spacings of the silicon substrate, it does not need to be traced back to NIST. If you buy a Mag-i-cal sample, there is included a letter copied from NIST to the developer of the sample, John McCaffrey, to that effect. I worked in a laboratory where we had to have traceable standards for QS-9000 certification. That is similar to ISO quality programs but for the automotive industry. The Mag-i-cal sample was accepted by the auditors because of that letter and the fact that the lengths scales can be traced back to the d-spacings of silicon.
The nice thing about using the Mag-i-cal sample for magnification calibration was that it could be used over the entire range of magnifications. That is not true for the other "standards" that are available, such as the lattice spacings of certain crystals, diffraction gratings, and polystyrene spheres. In my use of those, they were not consistent over the magnification ranges where two could be used. The Mag-i-cal sample does not have that problem. The other thing that is great about the Mag-i-cal sample is that it is very difficult to damage it with a beam.
Disclaimer: SBT sells the Mag-i-cal sample.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
Sent: Sunday, March 22, 2009 3:20 PM To: Walck-at-SouthBayTech.com
Dear All
My laboratory is participating in an electron microscopy length calibration exercise to understand the precision, accuracy and long-term reliability of distance measurements made with TEM.
Q1. I am aware that NIST have a standard procedure for calibrating SEMs and carrying out measurements, but am I correct is saying there is no equivalent for TEM - either from NIST or any other agency?
Q2. Am I also correct in believing there are no accredited distance calibration standards for TEM ie a certified standard produced by NIST or similar? I am aware of MagiCal, diffraction gratings and crystal lattices as references, but none of these are certified standards - note standards and references are often confused, but are not the same thing.
Q3. If anyone has carried out a similar exercise to this and has published the results, I'd be interested in any references.
Thanks and regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
==============================Original Headers============================== 12, 24 -- From david.mitchell-at-emu.usyd.edu.au Sun Mar 22 17:13:25 2009 12, 24 -- Received: from kabul.ucc.usyd.edu.au (kabul.ucc.usyd.edu.au [129.78.220.1]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2MMDOvC007699 12, 24 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Mar 2009 17:13:24 -0500 12, 24 -- Received: from kabul.ucc.usyd.edu.au (localhost [127.0.0.1]) 12, 24 -- by localhost (Postfix) with SMTP id 2D6CE5FDC2 12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST) 12, 24 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19]) 12, 24 -- by kabul.ucc.usyd.edu.au (Postfix) with ESMTP id 15CDB5FDB5 12, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 09:13:22 +1100 (EST) 12, 24 -- Received: from 172.17.185.135 ([172.17.185.135]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.133]) with Microsoft Exchange Server HTTP-DAV ; 12, 24 -- Sun, 22 Mar 2009 22:13:21 +0000 12, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 12, 24 -- Date: Mon, 23 Mar 2009 09:13:20 +1100 12, 24 -- Subject: RE: TEM length calibration standards 12, 24 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au} 12, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 24 -- Message-ID: {C5ED0330.597%david.mitchell-at-emu.usyd.edu.au} 12, 24 -- Thread-Topic: TEM length calibration standards 12, 24 -- Thread-Index: AcmrO2hqpzsbABcuEd6gAAAjMrpsqg== 12, 24 -- Mime-version: 1.0 12, 24 -- Content-type: text/plain; 12, 24 -- charset="US-ASCII" 12, 24 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008 5:29 PM
==============================Original Headers============================== 24, 23 -- From walck-at-southbaytech.com Mon Mar 23 15:13:28 2009 24, 23 -- Received: from nlpi053.prodigy.net (nlpi053.sbcis.sbc.com [207.115.36.82]) 24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2NKDSfr013059 24, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Mar 2009 15:13:28 -0500 24, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 24, 23 -- (authenticated bits=0) 24, 23 -- by nlpi053.prodigy.net (8.13.8 smtpauth/dk/map_regex/8.13.8) with ESMTP id n2NKDJX3000569; 24, 23 -- Mon, 23 Mar 2009 15:13:22 -0500 24, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 24, 23 -- To: {Microscopy-at-microscopy.com} 24, 23 -- Cc: {david.mitchell-at-emu.usyd.edu.au} 24, 23 -- References: {200903222219.n2MMJcsw012677-at-ns.microscopy.com} 24, 23 -- In-Reply-To: {200903222219.n2MMJcsw012677-at-ns.microscopy.com} 24, 23 -- Subject: RE: [Microscopy] RE: TEM length calibration standards 24, 23 -- Date: Mon, 23 Mar 2009 13:13:45 -0700 24, 23 -- Message-ID: {008001c9abf3$e09df260$a1d9d720$-at-com} 24, 23 -- MIME-Version: 1.0 24, 23 -- Content-Type: text/plain; 24, 23 -- charset="us-ascii" 24, 23 -- Content-Transfer-Encoding: 7bit 24, 23 -- X-Mailer: Microsoft Office Outlook 12.0 24, 23 -- Thread-Index: AcmrPEyzS+tFgNXTTuST+yUd2kc8FwAtbEVQ 24, 23 -- Content-Language: en-us ==============================End of - Headers==============================
We have a researcher who is studying the effects of a particular drug on rat liver, heart and spinal cord. Tissue for EM was processed with osmium tetroxide, en-bloc stained with uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy resin. Thin sections were stained with lead citrate. By EM we found increased amounts of glycogen in one of the liver samples. The researcher asked "How do you know it's glycogen". I said "It just is". He is worried that his supervisor won't accept that it's glycogen just because we said so. He wants to prove it.
So my question is...
Is there a stain for epoxy resin sections that will stain specifically for glycogen? Will periodic acid Schiff (PAS) work on resin-embedded sections?
Regards,
John Brealey
Supervisor - Electron Microscope Unit
E john.brealey-at-imvs.sa.gov.au
T 8222 6612
F 8222 6425
www.sapathology.sa.gov.au
SA Pathology (Queen Elizabeth Hospital)
Electron Microscope Unit, Surgical Pathology
SA Pathology
Queen Elizabeth Hospital
Woodville, 5011
AUSTRALIA
Quality Pathology supporting Training and Research
==============================Original Headers============================== 25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009 25, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O5qP3Q011161 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 00:52:26 -0500 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000"; 25, 27 -- d="scan'208";a="16015375" 25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 Mar 2009 16:22:24 +1030 25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id E9FE02EC078 for 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:22:23 +1030 (CST) 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030 25, 27 -- Organization: IMVS 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i} 25, 27 -- MIME-Version: 1.0 25, 27 -- Content-Type: text/plain; charset="us-ascii" 25, 27 -- Content-Transfer-Encoding: 7bit 25, 27 -- X-Mailer: Microsoft Office Outlook 11 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ== 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
In spite of beeing interesting by the concept of the ESEM since long ago, I had until now nor much time neither the real need to work on one. So I've a couple of questions about it.
1- First, most pictures done by ESEM in wet mode I've seen from collegues, either biologists or from material science, are done at high energy, 20-30 kV. It seems that the low energy "culture" is a bit ignored. (On the other hand, I agree that I'm perheps a bit addict to the low kV range !) But apart the subjecives aspects like "traditions", lab "culture" or the need of EDS, is there a more objective and technical reason for such choice ? Do the wet conditions limit so drastically the performences of the ESEM at low energy (lower then 5 keV f.ex.) ?
2- I a paper published in M&M 6-2000, Danilatos describes a very interesting evolution of the PLA (in particular for people interested in vaccum technology, like me...), called Reverse Flow Pressure Limiting Apperture. An annular gas jet flows from an intermediate stage at higher pressure into the specimen chamber, generating a pumping effect in the central part of the PLA and allowding to work with a greater differential pressure between the chamber and the column. I asked me if such a device has been soon integrated in commercial VP-SEMs, from FEI or other manufactuers. Danilatos mention too a cooperation with Zeiss on his website, but I never heard about a developpment like that by Zeiss.
Thanks for comments
Jacques
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hello, there is a Thiery's silverproteinate method for glycogen staining in ultrathin sections on EM grid: --------------------------- Thiery, J.-P. 1967. Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc. 6:987–1018. --------------------------
We have use it with success to proof glycogen-like polysaccharides in Streptomyces long time ago, (1996).
With regards Oldrich
-- Oldrich Benada Institute of Microbiology, Acad. Sci. CR, v.v.i. Videvská 1083 142 20 Prague 4 - Krc Czech Republic
On Tuesday 24 March 2009 06:55:32 am john.brealey-at-imvs.sa.gov.au wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --------------------------------------------------------------------------- } - } } Hi, } } We have a researcher who is studying the effects of a particular drug on } rat liver, heart and spinal cord. } Tissue for EM was processed with osmium tetroxide, en-bloc stained with } uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy } resin. Thin sections were stained with lead citrate. } By EM we found increased amounts of glycogen in one of the liver samples. } The researcher asked "How do you know it's glycogen". } I said "It just is". } He is worried that his supervisor won't accept that it's glycogen just } because we said so. He wants to prove it. } } So my question is... } } Is there a stain for epoxy resin sections that will stain specifically for } glycogen? } Will periodic acid Schiff (PAS) work on resin-embedded sections? } } Regards, } } John Brealey } } } Supervisor - Electron Microscope Unit } } } } E john.brealey-at-imvs.sa.gov.au } } T 8222 6612 } } F 8222 6425 } } www.sapathology.sa.gov.au } } } } SA Pathology (Queen Elizabeth Hospital) } } } } Electron Microscope Unit, Surgical Pathology } } SA Pathology } } Queen Elizabeth Hospital } } Woodville, 5011 } } AUSTRALIA } } } } Quality Pathology supporting Training and Research } } } } ==============================Original } Headers============================== 25, 27 -- From } john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009 25, 27 -- Received: } from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 25, 27 -- } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n2O5qP3Q011161 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 } 00:52:26 -0500 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000"; } 25, 27 -- d="scan'208";a="16015375" } 25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) } ([10.9.18.85]) 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; } 24 Mar 2009 16:22:24 +1030 25, 27 -- Received: from ablett.imvs.sa.gov.au } (10.20.98.41) by } 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP } Server id 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030 } 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au } [10.20.138.125]) by 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP } id E9FE02EC078 for 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 } 16:22:23 +1030 (CST) 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} } 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} } 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue } 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030 } 25, 27 -- Organization: IMVS } 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i} } 25, 27 -- MIME-Version: 1.0 } 25, 27 -- Content-Type: text/plain; charset="us-ascii" } 25, 27 -- Content-Transfer-Encoding: 7bit } 25, 27 -- X-Mailer: Microsoft Office Outlook 11 } 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ== } 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 26 -- From benada-at-biomed.cas.cz Tue Mar 24 03:41:10 2009 9, 26 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O8f9Cf021252 9, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 03:41:09 -0500 9, 26 -- Received: from u117ob.mbu.cas.cz (u117ob.mbu.cas.cz [147.231.44.101]) 9, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 26 -- (No client certificate requested) 9, 26 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id E47CF1A44571; 9, 26 -- Tue, 24 Mar 2009 09:41:08 +0100 (CET) 9, 26 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 9, 26 -- Reply-To: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz} 9, 26 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i. 9, 26 -- To: john.brealey-at-imvs.sa.gov.au 9, 26 -- Subject: Re: [Microscopy] Staining for glycogen in resin-embedded tissue 9, 26 -- Date: Tue, 24 Mar 2009 09:40:34 +0100 9, 26 -- User-Agent: KMail/1.9.9 9, 26 -- References: {200903240555.n2O5tWfm015327-at-ns.microscopy.com} 9, 26 -- In-Reply-To: {200903240555.n2O5tWfm015327-at-ns.microscopy.com} 9, 26 -- Cc: microscopy-at-microscopy.com 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Type: text/plain; 9, 26 -- charset="utf-8" 9, 26 -- Content-Disposition: inline 9, 26 -- Message-Id: {200903240940.34340.benada-at-biomed.cas.cz} 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2O8f9Cf021252 ==============================End of - Headers==============================
it is interesting to learn about "researchers" doubting about "experience" of long-time serious electron microscopist that have learned their job.
One has to admit that there ARE or probably COULD be some problems in "demonstrating" or localizing (= precipitating / retaining) glycogen particles within cellular structures, depending on the processing, especially the first steps of correct or at least "promising" fixation.
So, as to my knowledge, there are legion of recipes, special fixation methods and some tricks one can use to unequivocally demonstrate glycogen (particles). Nevertheless, judged only on morphological feature(s), sometimes it "might" be that ribosomal particles could be mistaken to be "perhaps" glycogen particles.
You should point your "researcher" to hundreds of papers (e.g.1965-1980&&) dealing with the specific localisation / demonstration of the stuff on ultrastructural grounds:
So here are my two Euro-cents: Using general sources: like also e.g. GEYER, G.(ed.), Ultrahistochemistry, 1973 (knowing that perhaps better standard books [Hayat's, Bozzola, Maunsbach etc.] are out there in the wild....):
- Tissue fixed with (FA-)GA & OsO4 alone: without positive staining ('contrasting'): no glycogen demo possible - Tissue fixed with (FA-)GA & OsO4, section staining with MJ Karnovsky's(1961)Pb-solution, or also KMnO4 (perhaps Mike Reedy will comment? on this because of unfavorable conditions if MNA is contained in the resin mixture) according to Lawn (1960), - Even the good old double staining (UO2Ac-Pb-citrate [Reynolds as well as Venable&Coggeshall) after "good" fixation are sufficient to achieve excellent glycogen staining (if it has not leached out/turned over during e.g. delayed fixation or use of poor primary fixative ). There (hereafter) also is to be mentioned the improvement in selective staining for glycosylated binding sites (i. e. glycogen, collagen, elastin) using the method of
Koert P. DINGEMANS and Marius A. van den BERGH WEERMAN ["Rapid Contrasting of Extracellular Elements in Thin Sections", Ultrastructural Pathology 14:519-527, 1990]. In their article one can learn a lot about staining properties and use of TANNIC Acid preincubation (prior to the classical "UO2-Pb-double staining") and the consequences in terms of increased e-density of sectioned substrate components.
I am not going into further detail for: - Glycogen staining with "Best's Carmine" (e.g. according to Themann H, 1960 J. Ultrastruct. Res 4, pp.401-412), - Glycogen staining using periodic acid incubation followed by staining with Reynolds Pb-citrate (according to PERRY MM, 1967) - Glycogen staining enhanced by using K3Fe*III*(CN)6 and(=in) 1%OsO4 (De Bruyn WC, 1968), - the PAT-Silver-proteinate Reaction (using Thiocarbohydrazide or Thiosemicarbazide) according to Thiery JP (1967), - the PA-PFPH-reaction according to BRADBURY S & STOWARD PJ (Histochemie 11,pp 71-80, 1967) etc., etc.
Being quite sure that there are a lot of other special techniques additionally available (certainly other listers will comment on......)
Addressing the probable failing of glycogen staining after UO2 Acetate-En Bloc staining (as you informed us about the specimen processing conditions:
Citing from DARDICK, Irving and Ian ROOB's CD-ROM-Edition:
PRIMER of Diagnostic Electron Microscopy for Pathologists - in - training, (Pathology Images Inc.,Ottawa, CANADA, (c) 2005 ISBN 0-9736518-0-6):
"Glycogen Glycogen, lipid and mitochondria collectively are involved in energy metabolism, and glycogen and lipid are reserve materials in this context. Glycogen forms granules which show variations in staining characteristics but which are typically electron-dense. At low power, aggregates of glycogen granules may present as "lakes" of amorphous material and only high magnification reveals the distinctive particulate substructure. Glycogen is easily leached out of cytoplasm, especially by uranyl acetate when used as an en bloc stain, and results in what appear to be "clear" spaces. Even in this circumstance, however, the electron microscopic features are characteristic enough to indicate that glycogen was originally present. Glycogen is an important diagnostic marker for both pediatric and adult tumors.
Glycogen granules are present as two cytoplasmic forms: the alpha-rosettes (typical of glycogen in liver cells) and the smaller monoparticulate beta-glycogen.
Legend Figure 1. A. (not included here): Cells from a rhabdomyoma in which large portions of the cytoplasm are occupied by what appear to be amorphous "lakes" of glycogen (G). X7,400. B. Cell from a small round-cell tumor showing focal areas of leached out glycogen (G) as a result of en bloc uranyl acetate staining. The homogeneous, rather glassy appearance is characteristic and there may be residual particulate glycogen at the margins of the glassy zones. X8,100.
Legend Figure 2. A. (not included here): Typical alpha-rosettes of glycogen in a hepatocyte filling the cytoplasm between mitochondria and a few segments of sER. X29,000. B. Monoparticulate beta-glycogen particles (20-30 nm across) filling the cytoplasmic spaces between mitochondria in a rhabdomyoblast. X84,000
Legend Figure 3. (not included here): Renal cell carcinoma with three adjacent tumor cells with accumulations of glycogen (G) in the cytoplasm. X9,200
Legend Figure 6. (not included here): Higher magnification of a Ewing's sarcoma tumor cell with an aggregate of the alpha-rosette form of glycogen (G) in the cytoplasm. X16,000. &, &, & "
Best wishes and good luck, Wolfgang Muss EM-Lab, Inst. Pathology SALK-PMU (Gen.Hospital, Private Paracelsus Medical University) Salzburg, Austria
} -----Ursprüngliche Nachricht----- } Von: john.brealey-at-imvs.sa.gov.au [mailto:john.brealey-at-imvs.sa.gov.au] } Gesendet: Dienstag, 24. März 2009 06:57 } An: Muß Wolfgang } Betreff: [Microscopy] Staining for glycogen in resin-embedded tissue } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } We have a researcher who is studying the effects of a } particular drug on rat liver, heart and spinal cord. } } Tissue for EM was processed with osmium tetroxide, en-bloc } stained with uranyl acetate, dehydrated with alcohols and } embedded in Procure 812 epoxy resin. } Thin sections were stained with lead citrate. } By EM we found increased amounts of glycogen in one of the } liver samples. } } The researcher asked "How do you know it's glycogen". I said } "It just is". } He is worried that his supervisor won't accept that it's } glycogen just because we said so. } He wants to prove it. } } So my question is... } } Is there a stain for epoxy resin sections that will stain } specifically for glycogen? } Will periodic acid Schiff (PAS) work on resin-embedded sections? } } Regards, } } John Brealey } } Supervisor - Electron Microscope Unit } } } E john.brealey-at-imvs.sa.gov.au } } T 8222 6612 } } F 8222 6425 } } www.sapathology.sa.gov.au } } } } SA Pathology (Queen Elizabeth Hospital) } } } } Electron Microscope Unit, Surgical Pathology } } SA Pathology } } Queen Elizabeth Hospital } } Woodville, 5011 } } AUSTRALIA } } } } Quality Pathology supporting Training and Research } } } } ==============================Original } Headers============================== } 25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009 } 25, 27 -- Received: from mailgate9.sa.gov.au } (mailgate9.sa.gov.au [203.26.121.14]) } 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n2O5qP3Q011161 } 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar } 2009 00:52:26 -0500 } 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000"; } 25, 27 -- d="scan'208";a="16015375" } 25, 27 -- Received: from unknown (HELO } EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) } 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 } Mar 2009 16:22:24 +1030 } 25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by } 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with } Microsoft SMTP Server id } 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030 } 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au } [10.20.138.125]) by } 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id } E9FE02EC078 for } 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 } 16:22:23 +1030 (CST) } 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} } 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} } 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue } 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030 } 25, 27 -- Organization: IMVS } 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i} } 25, 27 -- MIME-Version: 1.0 } 25, 27 -- Content-Type: text/plain; charset="us-ascii" } 25, 27 -- Content-Transfer-Encoding: 7bit } 25, 27 -- X-Mailer: Microsoft Office Outlook 11 } 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ== } 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 25, 36 -- From W.Muss-at-salk.at Tue Mar 24 04:38:39 2009 25, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 25, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O9ccGu009783 25, 36 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 04:38:38 -0500 25, 36 -- Received: from localhost (localhost [127.0.0.1]) 25, 36 -- by hermes.salk.at (Postfix) with ESMTP id 87760C383D; 25, 36 -- Tue, 24 Mar 2009 10:38:36 +0100 (CET) 25, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 25, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 25, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 25, 36 -- with ESMTP id bPNousV6-H1H; Tue, 24 Mar 2009 10:38:36 +0100 (CET) 25, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 25, 36 -- by hermes.salk.at (Postfix) with ESMTP id 1682FC382A; 25, 36 -- Tue, 24 Mar 2009 10:38:36 +0100 (CET) 25, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 25, 36 -- Tue, 24 Mar 2009 10:38:36 +0100 25, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 25, 36 -- Content-class: urn:content-classes:message 25, 36 -- MIME-Version: 1.0 25, 36 -- Content-Type: text/plain; 25, 36 -- charset="iso-8859-1" 25, 36 -- Subject: [Microscopy] Re: Staining for glycogen in resin-embedded tissue 25, 36 -- Date: Tue, 24 Mar 2009 10:38:35 +0100 25, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D08F5-at-N1RZ116.lksdom21.lks.local} 25, 36 -- In-Reply-To: {200903240557.n2O5vGaM017473-at-ns.microscopy.com} 25, 36 -- X-MS-Has-Attach: 25, 36 -- X-MS-TNEF-Correlator: 25, 36 -- Thread-Topic: [Microscopy] Re: Staining for glycogen in resin-embedded tissue 25, 36 -- Thread-Index: AcmsRWZplxy2w6F9S2OGOqwAXifM9QAD2edg 25, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 25, 36 -- To: {john.brealey-at-imvs.sa.gov.au} 25, 36 -- Cc: {microscopy-at-microscopy.com} 25, 36 -- X-OriginalArrivalTime: 24 Mar 2009 09:38:36.0144 (UTC) FILETIME=[4DF5EF00:01C9AC64] 25, 36 -- X-Scanned-By: SALK-Content-Filter 25, 36 -- Content-Transfer-Encoding: 8bit 25, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2O9ccGu009783 ==============================End of - Headers==============================
I was just going to replenish my stock of Polaroid Type 53 film (black & white, ASA800, medium contrast, 4x5" sheet film) and I find that Polaroid is not making it any more. Has anyone found a good substitute for this film? The ASA800 isn't important, a lower ASA is OK.
Thanks,
Diane Ciaburri ______________________________________ Diane Ciaburri Principal Materials Engineer General Dynamics 100 Plastics Ave., Rm 2517A Pittsfield MA 01201 phone: (413) 494-3430 email: diane.ciaburri-at-gd-ais.com ¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
==============================Original Headers============================== 5, 26 -- From prvs=1328d24a38=diane.ciaburri-at-gd-ais.com Tue Mar 24 10:45:48 2009 5, 26 -- Received: from camv02-relay2.casc.gd-ais.com (CAMV02-RELAY2.CASC.GD-AIS.COM [192.5.164.99]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OFjkq7006338 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 10:45:47 -0500 5, 26 -- Received: from ([10.73.100.22]) 5, 26 -- by camv02-relay2.casc.gd-ais.com with ESMTP id 5202701.157164724; 5, 26 -- Tue, 24 Mar 2009 08:37:42 -0700 5, 26 -- Received: from MAPF01-MAIL01.ad.gd-ais.com ([166.16.220.104]) by camv02-fes01.ad.gd-ais.com with Microsoft SMTPSVC(6.0.3790.3959); 5, 26 -- Tue, 24 Mar 2009 08:45:17 -0700 5, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 26 -- Content-class: urn:content-classes:message 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset="iso-8859-1" 5, 26 -- Subject: SEM (Old) - Polaroid Type 53 Film 5, 26 -- Date: Tue, 24 Mar 2009 11:45:14 -0400 5, 26 -- Message-ID: {1AFEAA87E04C0647A56AEA4BC5A73F3B03878B81-at-MAPF01-MAIL01.ad.gd-ais.com} 5, 26 -- X-MS-Has-Attach: 5, 26 -- X-MS-TNEF-Correlator: 5, 26 -- Thread-Topic: SEM (Old) - Polaroid Type 53 Film 5, 26 -- Thread-Index: Acmsl4YNYm/iHqxtSsaIeDSvZ6GQEA== 5, 26 -- From: "Ciaburri, Diane A." {Diane.Ciaburri-at-gd-ais.com} 5, 26 -- To: {Microscopy-at-microscopy.com} 5, 26 -- X-OriginalArrivalTime: 24 Mar 2009 15:45:17.0140 (UTC) FILETIME=[87938140:01C9AC97] 5, 26 -- Content-Transfer-Encoding: 8bit 5, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OFjkq7006338 ==============================End of - Headers==============================
Tungsten gun ESEM In a recent review someone said ESEM was like SEM but with extra difficulty. Not so much difficult as frustrating when viewing wet biological samples. Not being gold coated they give off fewer secondary electrons. At lower voltages the signal to noise ratio makes even modest magnifications hard to achieve. So, I confess, I have gone from being a 5kV high vacuum user to a 20kV wet ESEM user.
FEGESEM Still problematic - Kirk et al. (2009)* in an excellent review advocate 5-7kV.
Dave
* Kirk SE, Skepper JN Donald AM. 2009 Application of environmental scanning electron microscopy to determine surface structure. J Microsc 233:205-244.
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: 24 March 2009 08:39 To: David Patton
Hi all
In spite of beeing interesting by the concept of the ESEM since long ago, I had until now nor much time neither the real need to work on one. So I've a couple of questions about it.
1- First, most pictures done by ESEM in wet mode I've seen from collegues, either biologists or from material science, are done at high energy, 20-30 kV. It seems that the low energy "culture" is a bit ignored. (On the other hand, I agree that I'm perheps a bit addict to the low kV range !) But apart the subjecives aspects like "traditions", lab "culture" or the need of EDS, is there a more objective and technical reason for such choice ? Do the wet conditions limit so drastically the performences of the ESEM at low energy (lower then 5 keV f.ex.) ?
2- I a paper published in M&M 6-2000, Danilatos describes a very interesting evolution of the PLA (in particular for people interested in vaccum technology, like me...), called Reverse Flow Pressure Limiting Apperture. An annular gas jet flows from an intermediate stage at higher pressure into the specimen chamber, generating a pumping effect in the central part of the PLA and allowding to work with a greater differential pressure between the chamber and the column. I asked me if such a device has been soon integrated in commercial VP-SEMs, from FEI or other manufactuers. Danilatos mention too a cooperation with Zeiss on his website, but I never heard about a developpment like that by Zeiss.
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Question: We have a student interested in looking at the morphologies (fibre arrangement and spacing) of fibrin clots treated with various stabilizing agents as a bulk sample, not as a thin layer. I have tried a few different techniques- CPD vs HMDS, ROTO, holding the clot between dialysis membranes in a cartridge arrangement (for the record this was a miserable failure),and processing the clot formed in an Eppendorf tube- but continue to have issues with collapse of the clot.
We've done a lit search but haven't been able to find a definitive technique for a bulk clot. It is important to note this is an SEM application, and that TEM micrographs are not what the student would like to present in his paper.
I'm considering taking the clots to 100% EtOH and then freeze-drying, but my gut tells me the clots will still collapse.
I am wondering if it is even possible to keep a bulk clot expanded? Should fibrin be thought of as a cable, without strength in compression? If so, how have people worked around this in the past?
Yes there is! Yes it will! Actually I already answered the same question 3 weeks ago in this list. I copy my answer here:
----- Original Message ---- X-from: "john.brealey-at-imvs.sa.gov.au" {john.brealey-at-imvs.sa.gov.au} To: nizets2-at-yahoo.com Sent: Tuesday, March 24, 2009 6:58:02 AM
Hi,
We have a researcher who is studying the effects of a particular drug on rat liver, heart and spinal cord. Tissue for EM was processed with osmium tetroxide, en-bloc stained with uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy resin. Thin sections were stained with lead citrate. By EM we found increased amounts of glycogen in one of the liver samples. The researcher asked "How do you know it's glycogen". I said "It just is". He is worried that his supervisor won't accept that it's glycogen just because we said so. He wants to prove it.
So my question is...
Is there a stain for epoxy resin sections that will stain specifically for glycogen? Will periodic acid Schiff (PAS) work on resin-embedded sections?
Regards,
John Brealey
Supervisor - Electron Microscope Unit
E john.brealey-at-imvs.sa.gov.au
T 8222 6612
F 8222 6425
www.sapathology.sa.gov.au
SA Pathology (Queen Elizabeth Hospital)
Electron Microscope Unit, Surgical Pathology
SA Pathology
Queen Elizabeth Hospital
Woodville, 5011
AUSTRALIA
Quality Pathology supporting Training and Research
==============================Original Headers============================== 25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009 25, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O5qP3Q011161 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 00:52:26 -0500 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000"; 25, 27 -- d="scan'208";a="16015375" 25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 Mar 2009 16:22:24 +1030 25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id E9FE02EC078 for 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:22:23 +1030 (CST) 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030 25, 27 -- Organization: IMVS 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i} 25, 27 -- MIME-Version: 1.0 25, 27 -- Content-Type: text/plain; charset="us-ascii" 25, 27 -- Content-Transfer-Encoding: 7bit 25, 27 -- X-Mailer: Microsoft Office Outlook 11 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ== 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
==============================Original Headers============================== 43, 23 -- From nizets2-at-yahoo.com Tue Mar 24 13:49:21 2009 43, 23 -- Received: from web110807.mail.gq1.yahoo.com (web110807.mail.gq1.yahoo.com [67.195.13.230]) 43, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2OInJ0h024007 43, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 13:49:20 -0500 43, 23 -- Received: (qmail 90531 invoked by uid 60001); 24 Mar 2009 18:49:18 -0000 43, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237920558; bh=IQ4e4Pe+purZnbt8eOSy61OPZwfUSyyuUAa+7sFO8y0=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type; b=M+5QbBWWPN3GpVXeIeWTRUfISLKh9yKZoS0pukgo/5p/trO3bJt3jqABZ0auzWRwKOnBX4wC++aAeQSgGcGVh9VsBiKbh+9R6KgOrMMqutwd0ePLJ4k3rVrpWTkj9gDd9lsV+pkmQxZW9b3CPNprD16F9IxAKsXMbH5RAAckUKE= 43, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 43, 23 -- s=s1024; d=yahoo.com; 43, 23 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type; 43, 23 -- b=YIomuWpn07SWizLL/F292HK6jAcX0FBhayFJ8gZzLkOWknL2NLYHGqNr/oC4QMoCjNOy3Ssoaq6/csKNcwdsjn3m2wyXcspX7lOKqdzTclpgF1vij65seiXjQUCp6O95G0ncf1ZZs8TjY53ccL5Kx2CFMrY069lns3LCWDVsLYg=; 43, 23 -- Message-ID: {152072.90140.qm-at-web110807.mail.gq1.yahoo.com} 43, 23 -- X-YMail-OSG: .V_2ey0VM1miD1JTsxxJvM_sTOcYBWrRjgL6rNp_00D_6HVNbVxva1ONO.Ygut.zsa5luEeh37sR3I5Lma.3IFGV2jweN9Dn7ZW1LHBgrRKkQXn2f_R9IkE9LTqfder3er_sZFgAcSjKEcJ2rW7UJUhNeRrFNatNevkAQ3RhUlLjOtREVIWcWhHQfJX9zqUEBU5qccP9wAAvlEmzdrpY8lbAModByseR 43, 23 -- Received: from [80.121.65.0] by web110807.mail.gq1.yahoo.com via HTTP; Tue, 24 Mar 2009 11:49:18 PDT 43, 23 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1 43, 23 -- References: {200903240558.n2O5w21r018563-at-ns.microscopy.com} 43, 23 -- Date: Tue, 24 Mar 2009 11:49:18 -0700 (PDT) 43, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com} 43, 23 -- Subject: Re: [Microscopy] Staining for glycogen in resin-embedded tissue 43, 23 -- To: john.brealey-at-imvs.sa.gov.au 43, 23 -- Cc: microscopy-at-microscopy.com 43, 23 -- In-Reply-To: {200903240558.n2O5w21r018563-at-ns.microscopy.com} 43, 23 -- MIME-Version: 1.0 43, 23 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
I have asked the same question as you on the list some years ago and I got several answers. Here is the very straightforward protocol I finally used on my EPON sections of intestine and it worked, although the staining was not very intense. I didnt really spend much time to improve the method though.
- Periodic acid 5%: 30 min at 50°C - Schiff Reagent: 30 min at 50°C - Post-staining: Azur II mix (it is a 1:1 mix of methylene blue and Azur II, more stable than methylene alone): 20 min RT (section thickness: 300 nm)
Additionally, one person told me he stained the glycogen using reduced osmium. Another one gave me this reference: Shroeder et al. (1980) "An established routine method for differential staining of epoxy-embedded tissue sections" Microscopia Acta 83,111-116
Best of luck,
Stephane
----- Original Message ---- X-from: "john.brealey-at-imvs.sa.gov.au" {john.brealey-at-imvs.sa.gov.au} To: nizets2-at-yahoo.com Sent: Tuesday, March 24, 2009 6:58:02 AM
Hi,
We have a researcher who is studying the effects of a particular drug on rat liver, heart and spinal cord. Tissue for EM was processed with osmium tetroxide, en-bloc stained with uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy resin. Thin sections were stained with lead citrate. By EM we found increased amounts of glycogen in one of the liver samples. The researcher asked "How do you know it's glycogen". I said "It just is". He is worried that his supervisor won't accept that it's glycogen just because we said so. He wants to prove it.
So my question is...
Is there a stain for epoxy resin sections that will stain specifically for glycogen? Will periodic acid Schiff (PAS) work on resin-embedded sections?
Regards,
John Brealey
Supervisor - Electron Microscope Unit
E john.brealey-at-imvs.sa.gov.au
T 8222 6612
F 8222 6425
www.sapathology.sa.gov.au
SA Pathology (Queen Elizabeth Hospital)
Electron Microscope Unit, Surgical Pathology
SA Pathology
Queen Elizabeth Hospital
Woodville, 5011
AUSTRALIA
Quality Pathology supporting Training and Research
==============================Original Headers============================== 25, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 24 00:52:26 2009 25, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2O5qP3Q011161 25, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 00:52:26 -0500 25, 27 -- X-IronPort-AV: E=Sophos;i="4.38,411,1233495000"; 25, 27 -- d="scan'208";a="16015375" 25, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 25, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 24 Mar 2009 16:22:24 +1030 25, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 25, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 25, 27 -- 8.1.263.0; Tue, 24 Mar 2009 16:21:24 +1030 25, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 25, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id E9FE02EC078 for 25, 27 -- {Microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:22:23 +1030 (CST) 25, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 25, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 25, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 25, 27 -- Subject: Staining for glycogen in resin-embedded tissue 25, 27 -- Date: Tue, 24 Mar 2009 16:22:23 +1030 25, 27 -- Organization: IMVS 25, 27 -- Message-ID: {000001c9ac44$b44f2890$7d8a140a-at-41347i} 25, 27 -- MIME-Version: 1.0 25, 27 -- Content-Type: text/plain; charset="us-ascii" 25, 27 -- Content-Transfer-Encoding: 7bit 25, 27 -- X-Mailer: Microsoft Office Outlook 11 25, 27 -- Thread-Index: AcmsRLP2fLFNHWl5Tt6LMbAp/qqkrQ== 25, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
==============================Original Headers============================== 48, 25 -- From nizets2-at-yahoo.com Tue Mar 24 13:58:02 2009 48, 25 -- Received: from web110815.mail.gq1.yahoo.com (web110815.mail.gq1.yahoo.com [67.195.13.238]) 48, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2OIw0i4029486 48, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 13:58:01 -0500 48, 25 -- Received: (qmail 40779 invoked by uid 60001); 24 Mar 2009 18:57:59 -0000 48, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1237921078; bh=v22cQOqsnLT0xnbHVtJl5ZoNU8z6w4f6SyoXMD0AR1o=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=nNKR2ycOY8LhCon+Xc25bjQciGhNNgNyMj1Vr5cb54W3mlJQlRfcBkMBVzr1Z+/clzH9M3JHZOa7BoAbNDGIYFsO/uB3WhJNKn2Vs0vcaYOrL1ctEjFpIonUhpGWjC2ieFeZvfSGNMV/XZiPUGf7iWLETGCnrAB1zujoMI+AbWI= 48, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 48, 25 -- s=s1024; d=yahoo.com; 48, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 48, 25 -- b=14YObwpu+I9PJe1nb/z2SQfdqb3x24t4VsxBCLGRzzlEzqeFfB7b1kAjfV049xJpNvCTS6SIK0QsxKcKOMqUAIZPLXftkjOKsN8fBltwMpAc0sLUP5cgk/2uSXAok6sphZKnJMOX67IIx58kFLAg14YnJmjZU/eRhRWvoAsGc8s=; 48, 25 -- Message-ID: {983013.37372.qm-at-web110815.mail.gq1.yahoo.com} 48, 25 -- X-YMail-OSG: 6UTxgt8VM1kLAAOA9PsuYwPOqCaE.ygC7Sh1LGAaxDHAp7w3q7vOYxwAYcZEDkrVconjWnFVX0lq5Fa9GLdDLc5xsrGIp8OfW5aWBVvilNGAG77s_kFgxZ1siLn714FT840h.oDmadZC772k1jwM4PTAbbypzYcEbrvmdo0.3cbfVxWtJ8nzfSKeu.32Q6QEyya0UfjyqTlSs45ocNhc_L4MIPT8AC.EMZ6EzWk6Aa4lrRbI5upX1ac.E99eYylULlsuwoM5b5nXumif6x7aA_8- 48, 25 -- Received: from [80.121.65.0] by web110815.mail.gq1.yahoo.com via HTTP; Tue, 24 Mar 2009 11:57:58 PDT 48, 25 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1 48, 25 -- References: {200903240558.n2O5w21r018563-at-ns.microscopy.com} 48, 25 -- Date: Tue, 24 Mar 2009 11:57:58 -0700 (PDT) 48, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com} 48, 25 -- Subject: Re: [Microscopy] Staining for glycogen in resin-embedded tissue 48, 25 -- To: john.brealey-at-imvs.sa.gov.au 48, 25 -- Cc: microscopy-at-microscopy.com 48, 25 -- In-Reply-To: {200903240558.n2O5w21r018563-at-ns.microscopy.com} 48, 25 -- MIME-Version: 1.0 48, 25 -- Content-Type: text/plain; charset=iso-8859-1 48, 25 -- Content-Transfer-Encoding: 8bit 48, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OIw0i4029486 ==============================End of - Headers==============================
Diane, Your choice is digital capture or ... digital capture. If you have an EDS system, you may already have digital capture capability. There are some digital camera set-ups that keep using your record CRT or you can go to passive or active digital capture.
If you are interested in more info, please contact me off list.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Diane.Ciaburri-at-gd-ais.com [mailto:Diane.Ciaburri-at-gd-ais.com] Sent: Tuesday, March 24, 2009 11:53 AM To: kenconverse-at-qualityimages.biz
I was just going to replenish my stock of Polaroid Type 53 film (black & white, ASA800, medium contrast, 4x5" sheet film) and I find that Polaroid is not making it any more. Has anyone found a good substitute for this film? The ASA800 isn't important, a lower ASA is OK.
Thanks,
Diane Ciaburri ______________________________________ Diane Ciaburri Principal Materials Engineer General Dynamics 100 Plastics Ave., Rm 2517A Pittsfield MA 01201 phone: (413) 494-3430 email: diane.ciaburri-at-gd-ais.com ¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
==============================Original Headers============================== 5, 26 -- From prvs=1328d24a38=diane.ciaburri-at-gd-ais.com Tue Mar 24 10:45:48 2009 5, 26 -- Received: from camv02-relay2.casc.gd-ais.com (CAMV02-RELAY2.CASC.GD-AIS.COM [192.5.164.99]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OFjkq7006338 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 10:45:47 -0500 5, 26 -- Received: from ([10.73.100.22]) 5, 26 -- by camv02-relay2.casc.gd-ais.com with ESMTP id 5202701.157164724; 5, 26 -- Tue, 24 Mar 2009 08:37:42 -0700 5, 26 -- Received: from MAPF01-MAIL01.ad.gd-ais.com ([166.16.220.104]) by camv02-fes01.ad.gd-ais.com with Microsoft SMTPSVC(6.0.3790.3959); 5, 26 -- Tue, 24 Mar 2009 08:45:17 -0700 5, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 26 -- Content-class: urn:content-classes:message 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset="iso-8859-1" 5, 26 -- Subject: SEM (Old) - Polaroid Type 53 Film 5, 26 -- Date: Tue, 24 Mar 2009 11:45:14 -0400 5, 26 -- Message-ID: {1AFEAA87E04C0647A56AEA4BC5A73F3B03878B81-at-MAPF01-MAIL01.ad.gd-ais.com} 5, 26 -- X-MS-Has-Attach: 5, 26 -- X-MS-TNEF-Correlator: 5, 26 -- Thread-Topic: SEM (Old) - Polaroid Type 53 Film 5, 26 -- Thread-Index: Acmsl4YNYm/iHqxtSsaIeDSvZ6GQEA== 5, 26 -- From: "Ciaburri, Diane A." {Diane.Ciaburri-at-gd-ais.com} 5, 26 -- To: {Microscopy-at-microscopy.com} 5, 26 -- X-OriginalArrivalTime: 24 Mar 2009 15:45:17.0140 (UTC) FILETIME=[87938140:01C9AC97] 5, 26 -- Content-Transfer-Encoding: 8bit 5, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OFjkq7006338 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 25 -- From kenconverse-at-qualityimages.biz Tue Mar 24 14:26:21 2009 18, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OJQKRu020438 18, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 14:26:21 -0500 18, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com 18, 25 -- with ESMTP 18, 25 -- id {20090324192618.GWWR22116.cdptpa-omta02.mail.rr.com-at-Ken} ; 18, 25 -- Tue, 24 Mar 2009 19:26:18 +0000 18, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 25 -- To: {Diane.Ciaburri-at-gd-ais.com} , "MSA Listserver" {microscopy-at-microscopy.com} 18, 25 -- Subject: RE: [Microscopy] SEM (Old) - Polaroid Type 53 Film 18, 25 -- Date: Tue, 24 Mar 2009 15:26:13 -0400 18, 25 -- Message-ID: {FF845D85C5EE430FBA5FC4F7A87F71BE-at-Ken} 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="iso-8859-1" 18, 25 -- X-Priority: 3 (Normal) 18, 25 -- X-MSMail-Priority: Normal 18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 18, 25 -- Importance: Normal 18, 25 -- Thread-Index: AcmsmJO2nm7YAX6eS5ucdI7JNmeEywAHR1Mg 18, 25 -- In-Reply-To: {200903241552.n2OFqjwg014445-at-ns.microscopy.com} 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OJQKRu020438 ==============================End of - Headers==============================
Ritchie, Meant to get back to you sooner on this. The cheapest way to get to CL is to simply remove the Faraday Cage and scintillator from the secondary detector. That is your basic CL detector (monochrome). A dedicated CL detector generally has a larger diameter light pipe, but is still basically the back end of an E-T detector.
A color CL detector is a whole other can of worms and I don't know of a "cheap" way there.
I don't see why a CL detector couldn't be put on a desktop SEM, if there is a suitable port available and it can handle additional video inputs.
Have fun!
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: Wednesday, March 18, 2009 9:30 PM To: kenconverse-at-qualityimages.biz
Hi again
What's the cheapest way of getting into CL of quartz? Can CL be put onto a benchtop SEM?
cheers
Ritchie
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 6, 28 -- From r.sims-at-auckland.ac.nz Wed Mar 18 20:28:01 2009 6, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2J1RvM5030297 6, 28 -- for {microscopy-at-Microscopy.Com} ; Wed, 18 Mar 2009 20:28:01 -0500 6, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 6, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1BE859DFD1 6, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:27:56 +1300 (NZDT) 6, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 6, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 6, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 6, 28 -- with ESMTP id IwqwR4nhAL-X for {microscopy-at-Microscopy.Com} ; 6, 28 -- Thu, 19 Mar 2009 14:27:56 +1300 (NZDT) 6, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 6, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) 6, 28 -- (No client certificate requested) 6, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id EC4C59DFCB 6, 28 -- for {microscopy-at-Microscopy.Com} ; Thu, 19 Mar 2009 14:27:55 +1300 (NZDT) 6, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 6, 28 -- To: microscopy-at-Microscopy.Com 6, 28 -- Date: Thu, 19 Mar 2009 14:30:10 +1300 6, 28 -- MIME-Version: 1.0 6, 28 -- Subject: Benchtop CL 6, 28 -- Message-ID: {49C256F2.21397.17DDAB3-at-r.sims.auckland.ac.nz} 6, 28 -- Priority: normal 6, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 28 -- Content-type: text/plain; charset=US-ASCII 6, 28 -- Content-transfer-encoding: 7BIT 6, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From kenconverse-at-qualityimages.biz Tue Mar 24 14:38:11 2009 21, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OJcB0i002307 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 14:38:11 -0500 21, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com 21, 25 -- with ESMTP 21, 25 -- id {20090324193810.ECBC6142.cdptpa-omta03.mail.rr.com-at-Ken} ; 21, 25 -- Tue, 24 Mar 2009 19:38:10 +0000 21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 25 -- To: {r.sims-at-auckland.ac.nz} , "MSA Listserver" {microscopy-at-microscopy.com} 21, 25 -- Subject: RE: [Microscopy] Benchtop CL 21, 25 -- Date: Tue, 24 Mar 2009 15:38:06 -0400 21, 25 -- Message-ID: {C6DC472AAED54DE9B8A9D1F9C0A08D1D-at-Ken} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="us-ascii" 21, 25 -- X-Priority: 3 (Normal) 21, 25 -- X-MSMail-Priority: Normal 21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 21, 25 -- Importance: Normal 21, 25 -- Thread-Index: AcmoMkI+rHxXD/2dRDCCNeikjP4PbAEhGviw 21, 25 -- In-Reply-To: {200903190130.n2J1UF6K001811-at-ns.microscopy.com} 21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OJcB0i002307 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abrun-at-hsc.unt.edu Name: Anne-Marie Brun
Organization: UNT HSC at Fort Worth Texas 76107, USA
Title-Subject: [Filtered] digital cameras
Question: Hello everyone, We would like to switch from negative plates to digital capture. Our TEM is a Zeiss EM 910 instrument. We would like to have a digital camera with at least the same resolution as the film has. I'm sure there are several people out there who had to switch over to digital as we plan to do. What is the best way and what is the best camera and best price?
Thanks for your help. It is always good to listen to experts. Anne-Marie
I am interested in opinions on any available external systems (scan generators and stage controllers) that can be attached to Hitachi S4800 SEM.
We are trying to implement a system with external control of a piezo stage that would piggy-back on the main stage of the microscope. I know IXRF and Thermo offers this as a part of their EDS package and other EDS vendors might have similar systems for their mapping acquisitions. This is one route, what are the others? PI and attoCube have piezo-stages with controllers but no interface to a SEM software, are there others on the market with similar products?
Thanks in advance for you suggestions,
Jerzy *************************************************** Jerzy Gazda Ph.D. Section Manager - TEM, FIB, SEM Cerium Laboratories LLC 5204 E. Ben White Blvd. MS-512 Austin, TX 78741
(512) 934-5185 vm (512) 622-6600 pgr
www.ceriumlabs.com
==============================Original Headers============================== 8, 26 -- From Jerzy.Gazda-at-spansion.com Tue Mar 24 16:25:36 2009 8, 26 -- Received: from usausmgw01.spansion.com (usausmgw01.spansion.com [12.110.209.161]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2OLPaCt000501 8, 26 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2009 16:25:36 -0500 8, 26 -- X-IronPort-AV: E=McAfee;i="5300,2777,5563"; a="3788966" 8, 26 -- Received: from usausexbh1.spansion.com ([10.248.26.58]) 8, 26 -- by usausmgw01.spansion.com with ESMTP; 24 Mar 2009 14:25:35 -0700 8, 26 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by USAUSEXBH1.spansion.com with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Tue, 24 Mar 2009 16:25:35 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="iso-8859-1" 8, 26 -- Subject: Hitachi S4800 SEM external control to stage and image capture 8, 26 -- Date: Tue, 24 Mar 2009 16:25:35 -0500 8, 26 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF158738596-at-USAUSEXMBPF1.spansion.com} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: Hitachi S4800 SEM external control to stage and image capture 8, 26 -- Thread-Index: AcmsxxH61jPUnNX6RNC0fjsi4LpLMg== 8, 26 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com} 8, 26 -- To: {microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 24 Mar 2009 21:25:35.0948 (UTC) FILETIME=[122258C0:01C9ACC7] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2OLPaCt000501 ==============================End of - Headers==============================
} Diane, } Your choice is digital capture or ... digital capture. If you have an EDS } system, you may already have digital capture capability. There are some } digital camera set-ups that keep using your record CRT or you can go to } passive or active digital capture. } } If you are interested in more info, please contact me off list. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: Diane.Ciaburri-at-gd-ais.com [mailto:Diane.Ciaburri-at-gd-ais.com] } Sent: Tuesday, March 24, 2009 11:53 AM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] SEM (Old) - Polaroid Type 53 Film } } } } I was just going to replenish my stock of Polaroid Type 53 film (black & } white, ASA800, medium contrast, 4x5" sheet film) and I find that Polaroid is } not making it any more. Has anyone found a good substitute for this film? } The ASA800 isn't important, a lower ASA is OK. } } Thanks, } } Diane Ciaburri } ______________________________________ } Diane Ciaburri } Principal Materials Engineer } General Dynamics } 100 Plastics Ave., Rm 2517A } Pittsfield MA 01201 } phone: (413) 494-3430 } email: diane.ciaburri-at-gd-ais.com
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Dear Derrick During dehydration and drying the clot will collapse anyway. That is inevitable, unless you examine the specimen in wet conditions with ESEM, as people currently discuss in our listserver. In SEM, if the student wants to take measurements on the fibrin mesh he has to consider a ca 40% shrinkage of the specimen. To keep the mesh close to the original shape I think you have to attach the clot to a substrate that will also shrink -ideally some soft animal tissue- so it will not distort the mesh to any direction. Or, what about if you take out some red blood cells and leave more plasma to coagulate, dehydrate and CPD the bulk clot and then you look in SEM only at the the periphery of the clot -maybe that will be fine. Good luck! yorgos
eikonika-at-otenet.gr yorgosnikas-at-hotmail.com Tel/fax +30 210 8957677 Mobile +30 6945 107477
----- Original Message ----- X-from: {dhorne-at-interchange.ubc.ca} To: {eikonika-at-otenet.gr} Sent: Tuesday, March 24, 2009 7:19 PM
Hi
Just a few notes on digital systems as related to real life!
We have a "do it yourself" course that requires the client to take images of specific specimens and return the micrographs to us. Whilst most are able to have a pretty good shot at reaching the standard we require, I had a client who always turned out pictures which were identical, pictures that did not show the subtle changes that the practical was designed to produce.
Eventually on visiting the customer we found the images on screen to be superb and had they used sheet film the images would have been re produced on the film. Unfortunately they used a digital camera mounted above the viewing chamber which with its resolution and reduction in magnification was unable to define the differences that we required between the micrographs.
The above was very worrying! The aim of the practicals was to teach operators how the adjustment of the second condenser lens and determining the correct spot size has a considerable effect on the quality of the image. In addition basic Fresnel fringe images were not possible so poor was the resolution.
A warning, to ensure any level of image quality go for the maximum pixels in your camera if you intend to purchase an above screen side entry version.
Steve
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com -----Original Message----- X-from: abrun-at-hsc.unt.edu [mailto:abrun-at-hsc.unt.edu] Sent: 24 March 2009 20:40 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abrun-at-hsc.unt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abrun-at-hsc.unt.edu Name: Anne-Marie Brun
Organization: UNT HSC at Fort Worth Texas 76107, USA
Title-Subject: [Filtered] digital cameras
Question: Hello everyone, We would like to switch from negative plates to digital capture. Our TEM is a Zeiss EM 910 instrument. We would like to have a digital camera with at least the same resolution as the film has. I'm sure there are several people out there who had to switch over to digital as we plan to do. What is the best way and what is the best camera and best price?
Thanks for your help. It is always good to listen to experts. Anne-Marie
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ahmad_ds-at-yahoo.com Name: Ahmad Ashkhaibi
Organization: Al-Balqa Applied University
Title-Subject: [Filtered] EDS window
Question: I work on and EDS manufactured by EDAX of the model XL-30 SUTW. I want to know what is the material of fabrication of the super ultra-thin window of the EDS I have.
Hi I have frequently imaged wet samples at 5kV and image quality can be variable depending on the sample. Very flat well attached cells on glass are some of the more problematic. But using higher kV means you hardly see the cells. However just yesterday I looked at some confluent layers of mineralising cells on thermanox at 5kV and 4-5 torr with little difficulty (but it is a FEG) Always ask researchers for extra specimens if possible to play with and optimise conditions. I routinely start low and work up to higher kV if necessary.
------------
Tungsten gun ESEM In a recent review someone said ESEM was like SEM but with extra difficulty. Not so much difficult as frustrating when viewing wet biological samples. Not being gold coated they give off fewer secondary electrons. At lower voltages the signal to noise ratio makes even modest magnifications hard to achieve. So, I confess, I have gone from being a 5kV high vacuum user to a 20kV wet ESEM user.
FEGESEM Still problematic - Kirk et al. (2009)* in an excellent review advocate 5-7kV.
Dave
* Kirk SE, Skepper JN Donald AM. 2009 Application of environmental scanning electron microscopy to determine surface structure. J Microsc 233:205-244.
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: 24 March 2009 08:39 To: David Patton
Hi all
In spite of beeing interesting by the concept of the ESEM since long ago, I had until now nor much time neither the real need to work on one. So I've a couple of questions about it.
1- First, most pictures done by ESEM in wet mode I've seen from collegues, either biologists or from material science, are done at high energy, 20-30 kV. It seems that the low energy "culture" is a bit ignored. (On the other hand, I agree that I'm perheps a bit addict to the low kV range !) But apart the subjecives aspects like "traditions", lab "culture" or the need of EDS, is there a more objective and technical reason for such choice ? Do the wet conditions limit so drastically the performences of the ESEM at low energy (lower then 5 keV f.ex.) ?
2- I a paper published in M&M 6-2000, Danilatos describes a very interesting evolution of the PLA (in particular for people interested in vaccum technology, like me...), called Reverse Flow Pressure Limiting Apperture. An annular gas jet flows from an intermediate stage at higher pressure into the specimen chamber, generating a pumping effect in the central part of the PLA and allowding to work with a greater differential pressure between the chamber and the column. I asked me if such a device has been soon integrated in commercial VP-SEMs, from FEI or other manufactuers. Danilatos mention too a cooperation with Zeiss on his website, but I never heard about a developpment like that by Zeiss.
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==============================Original Headers============================== 23, 32 -- From Nicola.Weston-at-nottingham.ac.uk Wed Mar 25 08:19:36 2009 23, 32 -- Received: from smtp2.nottingham.ac.uk (smtp2.nottingham.ac.uk [128.243.44.5]) 23, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PDJY0l003066 23, 32 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 08:19:35 -0500 23, 32 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 23, 32 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 23, 32 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 23, 32 -- id 1LmT0E-0003PW-4O 23, 32 -- for microscopy-at-microscopy.com; Wed, 25 Mar 2009 13:18:42 +0000 23, 32 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 23, 32 -- Wed, 25 Mar 2009 13:18:40 +0000 23, 32 -- x-mimeole: Produced By Microsoft Exchange V6.5 23, 32 -- Content-class: urn:content-classes:message 23, 32 -- MIME-Version: 1.0 23, 32 -- Content-Type: text/plain; 23, 32 -- charset="iso-8859-1" 23, 32 -- Subject: Quest. about the ESEM 23, 32 -- Date: Wed, 25 Mar 2009 13:18:40 -0000 23, 32 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D940120F1E2-at-VUIEXCHA.ad.nottingham.ac.uk} 23, 32 -- X-MS-Has-Attach: 23, 32 -- X-MS-TNEF-Correlator: 23, 32 -- Thread-Topic: Quest. about the ESEM 23, 32 -- Thread-Index: AcmtTDaSAkqv7pZtTqqlHCQUXxTF+w== 23, 32 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 23, 32 -- To: {microscopy-at-microscopy.com} 23, 32 -- X-OriginalArrivalTime: 25 Mar 2009 13:18:40.0992 (UTC) FILETIME=[37139600:01C9AD4C] 23, 32 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 23, 32 -- X-UoN-MailScanner: Found to be clean 23, 32 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 23, 32 -- X-Spam-Status: No 23, 32 -- Content-Transfer-Encoding: 8bit 23, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PDJY0l003066 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Technique for Preparation of Polymer Film Cross Sections
Question: Does anyone have a good technique for preparation of (cryo) cross sections of polymer films for SEM examination? I am interested in viewing the distribution of two polymers in a polymer blend.
Wendy Cheng, Ph.D. International Paper 6283 Tri-Ridge Blvd. Loveland, OH 45140
I've always just dropped the polymer samples in liquid nitrogen, grabbed and snapped. I did have one nylon sample that required repeated flexing before it would break, even at LN2 temperatures, but I still got good fractures for examing the cross-section. For thin films ... on paper? Snapping in LN2 worked there as well. If the films are separate, then you may need to clamp them between two thicker (but not thick) supporting pieces and snap the sandwich. This also works for thin films laminated (with bonding) between two thicker polymer pieces, although they will often delaminate (which can be useful). For films on substrates, it can make a difference whether the samples are snapped "toward" or "away" from the film, that is, whether the film is broken in compression ("toward") or tension ("away"). I can't give a generic one is better than the other, but I try breaking the film in tension first. Thick(ish) substrates may require cutting/scoring/sawing 1/2 way (or more) through first, from the side opposite the film.
Phil
} Email: wendy.cheng-at-ipaper.com } Name: Wendy Cheng } } Organization: International Paper } } Title-Subject: [Filtered] Technique for Preparation of Polymer Film } Cross Sections } } Question: Does anyone have a good technique for preparation of (cryo) } cross sections of polymer films for SEM examination? I am interested } in viewing the distribution of two polymers in a polymer blend. } } Wendy Cheng, Ph.D. } International Paper } 6283 Tri-Ridge Blvd. } Loveland, OH 45140 } } Phone: 513-248-6698 } email: wendy.cheng-at-ipaper.com -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 27 -- From oshel1pe-at-cmich.edu Wed Mar 25 09:12:49 2009 4, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PECm6b032063 4, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 09:12:49 -0500 4, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n2PECd56005028; 4, 27 -- Wed, 25 Mar 2009 10:12:40 -0400 4, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 27 -- Wed, 25 Mar 2009 10:12:33 -0400 4, 27 -- Mime-Version: 1.0 4, 27 -- Message-Id: {f06240800c5efe99479c2-at-[141.209.160.249]} 4, 27 -- In-Reply-To: {200903251350.n2PDoDqi023551-at-ns.microscopy.com} 4, 27 -- References: {200903251350.n2PDoDqi023551-at-ns.microscopy.com} 4, 27 -- Date: Wed, 25 Mar 2009 10:12:31 -0400 4, 27 -- To: wendy.cheng-at-ipaper.com 4, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 27 -- Subject: Re: [Microscopy] viaWWW: Technique for Preparation of Polymer 4, 27 -- Film Cross Sections 4, 27 -- Cc: Microscopy-at-microscopy.com 4, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 27 -- X-OriginalArrivalTime: 25 Mar 2009 14:12:33.0204 (UTC) FILETIME=[BD9FFF40:01C9AD53] 4, 27 -- X-Canit-CHI2: 0.00 4, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 27 -- X-Spam-Score: -3.70 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,_L_SUPPORT,Bayes(0.0001,-0.5) 4, 27 -- X-CanItPRO-Stream: default 4, 27 -- X-Canit-Stats-ID: 10755722 - 7e12acab6491 4, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Hello Wendy, At the tire company I worked at we used to mount samples in a puddle of mucilage gun and water (50/50) and freeze with LN2. The samples were cut with glass knives for thin sections, but we also used a glass knife to level the surface for SEM. You have to work quickly as the brass/Teflon mounting block was the only source of cold, if one can speak of a source of cold.
We also used a cryo-microtome in the same manner. Mount sample in the water mucilage media, freeze and face off the block with a diamond knife.
I believed we used a 50/50 water solution of DMSO to lubricate the diamond edge.
Most things should cut well if you’re below the Tg point. Most plastics have a Tg above room temp. Have fun…
I would suspect that if you are looking for phase morphology, you might want to try etching one phase over the other, see Sawyer’s book on polymer microscopy. I’ve had some success with vapor phase staining with OsO4, assuming some unsaturation in one of the polymer phases.
stay safe........ Frank
wendy.cheng-at-ipape r.com To 03/25/2009 09:54 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] viaWWW: Technique for wendy.cheng-at-ipape Preparation of Polymer Film Cross r.com Sections
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Title-Subject: [Filtered] Technique for Preparation of Polymer Film Cross Sections
Question: Does anyone have a good technique for preparation of (cryo) cross sections of polymer films for SEM examination? I am interested in viewing the distribution of two polymers in a polymer blend.
Wendy Cheng, Ph.D. International Paper 6283 Tri-Ridge Blvd. Loveland, OH 45140
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Wed Mar 25 08:45:39 2009 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PDjbTk017677 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 08:45:38 -0500 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p0624080dc5efe5f4bc62-at-[206.69.208.22]} 8, 11 -- Date: Wed, 25 Mar 2009 08:45:36 -0500 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: wendy.cheng-at-ipaper.com (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: Technique for Preparation of Polymer Film Cross Sections 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 30, 25 -- From frank_karl-at-lincolnelectric.com Wed Mar 25 09:19:06 2009 30, 25 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 30, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PEJ55k008985 30, 25 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 09:19:05 -0500 30, 25 -- In-Reply-To: {200903251354.n2PDsJLX030190-at-ns.microscopy.com} 30, 25 -- Subject: Re: [Microscopy] viaWWW: Technique for Preparation of Polymer Film Cross 30, 25 -- Sections 30, 25 -- To: wendy.cheng-at-ipaper.com, Microscopy-at-microscopy.com 30, 25 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 30, 25 -- Message-ID: {OF37566D8A.EAF18CE1-ON85257584.004E8199-85257584.004E9FEE-at-lincolnelectric.com} 30, 25 -- Date: Wed, 25 Mar 2009 10:18:49 -0400 30, 25 -- From: Frank_Karl-at-lincolnelectric.com 30, 25 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 30, 25 -- 07, 2008) at 03/25/2009 10:18:47 AM, 30, 25 -- CD-MIME complete at 03/25/2009 10:18:47 AM, 30, 25 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 30, 25 -- 07, 2008) at 03/25/2009 10:18:47 AM, 30, 25 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 30, 25 -- 07, 2008) at 03/25/2009 10:18:47 AM, 30, 25 -- Serialize complete at 03/25/2009 10:18:47 AM 30, 25 -- MIME-Version: 1.0 30, 25 -- Content-Type: text/plain; 30, 25 -- charset="UTF-8" 30, 25 -- Content-Transfer-Encoding: 8bit 30, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n2PEJ55k008985 ==============================End of - Headers==============================
Hi John and Listers, Wanted to add two comments to discussion of retaining and identifying glycogen in tissue.
1) Several years ago there was a thread on the list about glycogen with primary input from Krystyna Rybicka. She maintained that the protein component associated with glycogen was subsequently washed out in dehydration if tissue was subjected to a pH change (ie. en bloc staining with UA) during processing. The remaining free-floating glycogen could then clump in the cell. She recommended avoiding that pH change during processing to retain the classic rosette structure. Once embedded, the PAS technique could be used for detection of glycogen on the LM level and the Thiery technique (which I think Wolfgang Muss mentioned) could be used for EM.
Here are two references: a) K.K. Rybicka. 1996. Tissue & Cell 28 (3) 253-267. b) Microscopy Today, October 1994. "Glycogen Granules Revisited".
2) Many years ago when I worked at the Dana-Farber Cancer Institute we found that the following protocol was very useful in preserving glycogen rosettes in liver biopsies of pediatric cancer patients. It involved fixing the tissue with osmium potassium ferrocyanide. The recipe was:
The OPF solution was prepared with thorough mixing just prior to use and administered to tissue for two hours in refrigerator. The general processing protocol was glut. and OPF fixations, alcohol and propylene oxide dehydrations, and epon embedment. We post-stained with UA and Reynolds lead citrate. UA enbloc staining is not recommended.
So I guess there are two important issues: preservation of glycogen in the protein-bound form and post-embedding identification by the Thiery technique, for example. I would think that the observance of rosettes is strong enough evidence. Don
Donald Gantz Research Histologist/Electron Microscopist Dept. Physiology and Biophysics Boston University School of Medicine email: gantz-at-bu.edu phone: 617-638-4017
----- Original Message ----- X-from: {john.brealey-at-imvs.sa.gov.au} To: {gantz-at-bu.edu} Sent: Tuesday, March 24, 2009 12:53 AM
Nicola, My experience with mineralizing cell cultures in ESEM is not really good. Observation of cells without fixation is not possible (vaporizing of buffer leaves a lot of crystals on surfaces of specimens). Fixed cultures could be washed in distilled water. To keep cells wet they should be cooled to temperatures 2-5 degrees Celsius and ESEM should be operated at pressures above 5 torr (to be close to a dew point). A bit above dew point - and cells will be covered with a layer of water, a bit below dew point - and cells will be dehydrated rapidly (they are tiny). Since balancing exactly at dew point is practically impossible, we are working in ESEM with slowly or rapidly (as for cell cultures) dehydrating specimens. So, what we can really see in ESEM, working with cell cultures, are fixed cells, dehydrated in a microscope chamber. May be epithelium cells can resist dehydration for a while, but I did not have experience with them.
Speaking about other specimens of bigger volume/surface ratio I try to work fast, or, if necessary, I periodically go above dew point (changing pressure) to rehydrate specimens. In my experience with field-emission XL30, going below 5 kV makes signal too noisy, so I use voltages from 5 kV to 15 kV (mostly 10 kV).
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
I cannot understand "addiction" to any kV range. In my work I routinely use all voltages my microscope can supply: from 300 V for easily damaged or charging specimens to 30 kV for "special effects". For example, at 30 kV I can get pictures of yeas colonies with white cells on black background (useful for analysis of shapes of colonies). At 15 kV I can make pictures with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20 kV could be useful in imaging fractures of multiphase specimens like composites and metals, when phases are highlighted. And so on.
As for ESEM, low voltages ( {5kV for my microscope) are not really useful because of high noise level.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} Hi all } } In spite of beeing interesting by the concept of the ESEM } since long ago, I had until now nor much time neither the } real need to work on one. } So I've a couple of questions about it. } } 1- First, most pictures done by ESEM in wet mode I've seen } from collegues, either biologists or from material science, } are done at high energy, 20-30 kV. It seems that the low } energy "culture" is a bit ignored. (On the other hand, I } agree that I'm perheps a bit addict to the low kV range !) } But apart the subjecives aspects like "traditions", lab } "culture" or the need of EDS, is there a more objective and } technical reason for such choice ? Do the wet conditions } limit so drastically the performences of the ESEM at low } energy (lower then 5 keV } f.ex.) ? } } 2- I a paper published in M&M 6-2000, Danilatos describes a } very interesting evolution of the PLA (in particular for } people interested in vaccum technology, like me...), called } Reverse Flow Pressure Limiting Apperture. An annular gas jet } flows from an intermediate stage at higher pressure into the } specimen chamber, generating a pumping effect in the central } part of the PLA and allowding to work with a greater } differential pressure between the chamber and the column. } I asked me if such a device has been soon integrated in } commercial VP-SEMs, from FEI or other manufactuers. Danilatos } mention too a cooperation with Zeiss on his website, but I } never heard about a developpment like that by Zeiss. } } Thanks for comments } } Jacques } } -- } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } ==============================Original } Headers============================== } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24 } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr } (mailhost.u-strasbg.fr [130.79.200.157]) } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n2O8ZMax011825 } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar } 2009 03:35:23 -0500 } 9, 29 -- Received: from ipcms.u-strasbg.fr } (ipcms.u-strasbg.fr [130.79.210.2]) } 9, 29 -- by mailhost.u-strasbg.fr } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280 } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 } Mar 2009 09:35:21 +0100 (CET) } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr } [130.79.152.3]) } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA } (256/256 bits)) } 9, 29 -- (No client certificate requested) } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id } 5F52D3EC002 } 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar } 2009 09:34:14 +0100 (CET) } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr} } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From: } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9, } 29 -- MIME-Version: 1.0 9, 29 -- To: } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain; } charset=ISO-8859-1; format=flowed 9, 29 -- } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: } jacques.faerber-at-ipcms.u-strasbg.fr } 9, 29 -- Subject: Quest. about the ESEM } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by } milter-greylist-4.0.1 (mailhost.u-strasbg.fr } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9, } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32 } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 } -- X-Spam-Status: No, score=-100.0 required=5.0 } tests=USER_IN_WHITELIST } 9, 29 -- autolearn=disabled version=3.2.5 } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 } (2008-06-10) on mr7.u-strasbg.fr } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PG4PlI026286 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26 -0500 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="iso-8859-1" 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu} 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: {Microscopy-at-microscopy.com} 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC) FILETIME=[5D87D3C0:01C9AD63] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PG4PlI026286 ==============================End of - Headers==============================
I agree fully with your comment. I said "addict" as a joke, as in our needs I'm so often in situation where I say to my collegues that we can try with higher kV, but that I "feel" that it will give nothing. And so it is, we try and finish between 1-5 keV. So I ask me myself sometimes if it's an "a priori" or a addiction ! While I say always to the students that they must try, to find the right energy for the right illustration of their observations.
But... in the daily work, our samples are most thin metallic films, oxyde nanoparticules, C nanotubes, etc, with the need of high magnifications. No mineralogy any more, nor metallography, only small thin light stuff which gives in most cases no usable pictures at 15 keV or more.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
DusevichV-at-umkc.edu a écrit : } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jacques, } } I cannot understand "addiction" to any kV range. In my work I routinely use all voltages my microscope can supply: from 300 V for easily damaged or charging specimens to 30 kV for "special effects". For example, at 30 kV I can get pictures of yeas colonies with white cells on black background (useful for analysis of shapes of colonies). At 15 kV I can make pictures with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20 kV could be useful in imaging fractures of multiphase specimens like composites and metals, when phases are highlighted. And so on. } } As for ESEM, low voltages ( {5kV for my microscope) are not really useful because of high noise level. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } Hi all } } } } In spite of beeing interesting by the concept of the ESEM } } since long ago, I had until now nor much time neither the } } real need to work on one. } } So I've a couple of questions about it. } } } } 1- First, most pictures done by ESEM in wet mode I've seen } } from collegues, either biologists or from material science, } } are done at high energy, 20-30 kV. It seems that the low } } energy "culture" is a bit ignored. (On the other hand, I } } agree that I'm perheps a bit addict to the low kV range !) } } But apart the subjecives aspects like "traditions", lab } } "culture" or the need of EDS, is there a more objective and } } technical reason for such choice ? Do the wet conditions } } limit so drastically the performences of the ESEM at low } } energy (lower then 5 keV } } f.ex.) ? } } } } 2- I a paper published in M&M 6-2000, Danilatos describes a } } very interesting evolution of the PLA (in particular for } } people interested in vaccum technology, like me...), called } } Reverse Flow Pressure Limiting Apperture. An annular gas jet } } flows from an intermediate stage at higher pressure into the } } specimen chamber, generating a pumping effect in the central } } part of the PLA and allowding to work with a greater } } differential pressure between the chamber and the column. } } I asked me if such a device has been soon integrated in } } commercial VP-SEMs, from FEI or other manufactuers. Danilatos } } mention too a cooperation with Zeiss on his website, but I } } never heard about a developpment like that by Zeiss. } } } } Thanks for comments } } } } Jacques } } } } -- } } J. Faerber } } IPCMS-GSI } } (Institut de Physique et Chimie des Matériaux de Strasbourg } } Groupe Surface et Interfaces) 23, rue de Loess ; BP43 } } 67034 Strasbourg CEDEX 2 } } France } } } } Tel 00 33(0)3 88 10 71 01 } } Fax 00 33(0)3 88 10 72 48 } } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } } } ==============================Original } } Headers============================== } } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24 } } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr } } (mailhost.u-strasbg.fr [130.79.200.157]) } } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n2O8ZMax011825 } } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar } } 2009 03:35:23 -0500 } } 9, 29 -- Received: from ipcms.u-strasbg.fr } } (ipcms.u-strasbg.fr [130.79.210.2]) } } 9, 29 -- by mailhost.u-strasbg.fr } } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280 } } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 } } Mar 2009 09:35:21 +0100 (CET) } } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr } } [130.79.152.3]) } } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA } } (256/256 bits)) } } 9, 29 -- (No client certificate requested) } } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id } } 5F52D3EC002 } } 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar } } 2009 09:34:14 +0100 (CET) } } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr} } } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From: } } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9, } } 29 -- MIME-Version: 1.0 9, 29 -- To: } } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain; } } charset=ISO-8859-1; format=flowed 9, 29 -- } } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: } } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: } } jacques.faerber-at-ipcms.u-strasbg.fr } } 9, 29 -- Subject: Quest. about the ESEM } } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by } } milter-greylist-4.0.1 (mailhost.u-strasbg.fr } } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9, } } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32 } } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 } } -- X-Spam-Status: No, score=-100.0 required=5.0 } } tests=USER_IN_WHITELIST } } 9, 29 -- autolearn=disabled version=3.2.5 } } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 } } (2008-06-10) on mr7.u-strasbg.fr } } ==============================End of - } } Headers============================== } } } } } } } } } ==============================Original Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009 } 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PG4PlI026286 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500 } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="iso-8859-1" } 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM } 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500 } 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM } 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA } 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 8, 25 -- To: {Microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC) FILETIME=[5D87D3C0:01C9AD63] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PG4PlI026286 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Mar 25 12:05:42 2009 9, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PH5fou009666 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:05:41 -0500 9, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n2PH5ct7066933 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 18:05:38 +0100 (CET) 9, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 31 -- (No client certificate requested) 9, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 3111C3EC001 9, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Mar 2009 18:04:25 +0100 (CET) 9, 31 -- Message-ID: {49CA6455.8040403-at-ipcms.u-strasbg.fr} 9, 31 -- Date: Wed, 25 Mar 2009 18:05:25 +0100 9, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 31 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318) 9, 31 -- MIME-Version: 1.0 9, 31 -- To: Microscopy-at-microscopy.com 9, 31 -- Subject: Re: [Microscopy] RE: Quest. about the ESEM 9, 31 -- References: {200903251609.n2PG9P5u001558-at-ns.microscopy.com} 9, 31 -- In-Reply-To: {200903251609.n2PG9P5u001558-at-ns.microscopy.com} 9, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-IPCMS-MailScanner: Found to be clean 9, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 9, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 25 Mar 2009 18:05:38 +0100 (CET) 9, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9165/Wed Mar 25 16:08:41 2009 on mr2.u-strasbg.fr 9, 31 -- X-Virus-Status: Clean 9, 31 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST 9, 31 -- autolearn=disabled version=3.2.5 9, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr2.u-strasbg.fr ==============================End of - Headers==============================
If you are working in cryo mode inject the solution into a narrow drinking straw about 1/2 inch tall that is mounted in your cryo holder. Freeze the unit and then crack it open in your cryo manipulation chamber. I used glass rods for this in the early days of cryo but found that the very narrow drinking straws are far better. The best are those that are often used as stirrers for do it yourself coffee making!
I think we are in the same area as a few weeks ago when we were talking about looking at cross sections but only if you could crack them in air?
If the material will solidify in air cast it onto a piece of aluminium foil. Once dry place the foil in liquid nitrogen and then collect the media as it cracks off the foil. The different contraction rates of foil and media will cause the media to crack into nice cross sections.
If the material is quite stiff or may be mounted firmly on a stiff surface cut it down to about 1 1/4inch by 3/8 inch. Place it in liquid nitrogen until the solution stops bubbling. Remove the specimen and bend it until it cracks. If it will not crack neck the material at the half way point and try again. For more details and diagrams look at www.emcourses.com/crack.htm
When a material will not fracture naturally and you are unable to use a cryo device you need to stiffen the material. If the material is not soluble in water we use a water soluble carbon solution as the stiffener for example Agar G303. Drill a fine (1/8th inch) hole though two stubs placed face to face. Place your fibres/material through the hole and fill the additional space with the carbon solution. When dry plunge the unit into liquid nitrogen and when the liquid stops boiling take the unit out and crack it by striking the interface with a single edged blade. Take great care not to cut through the nit, simple crack it open.
Once the two units are back at room temperature and the condensation has dispersed they are usable in the light microscope and the SEM.
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: wendy.cheng-at-ipaper.com [mailto:wendy.cheng-at-ipaper.com] Sent: 25 March 2009 13:47 To: protrain-at-emcourses.com
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Title-Subject: [Filtered] Technique for Preparation of Polymer Film Cross Sections
Question: Does anyone have a good technique for preparation of (cryo) cross sections of polymer films for SEM examination? I am interested in viewing the distribution of two polymers in a polymer blend.
Wendy Cheng, Ph.D. International Paper 6283 Tri-Ridge Blvd. Loveland, OH 45140
Taking up the comments about(E)SEM accelerating voltage and how it should be used I am often tempted to ask if a laboratory runs by tradition or by science, unfortunately too many run by tradition!
In my mind the most important question an SEM operator has to answer is "Which kV?" The correct answer in my mind is "The kV that displays the most information about the specimen." However I often find that to obtain the most information about a specimen it is good to view it at higher and lower accelerating voltages - my choice 2 & 10 or 5 and 15.
The only time I have my students use the highest accelerating voltages are when using BSE techniques. We have worked as low as 100 volts on a tungsten hairpin instrument, a 15 year old model in fact!
The motto "Microscopists are scientists they should experiment."
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: 25 March 2009 17:08 To: protrain-at-emcourses.com
Hi Vladimir
I agree fully with your comment. I said "addict" as a joke, as in our needs I'm so often in situation where I say to my collegues that we can try with higher kV, but that I "feel" that it will give nothing. And so it is, we try and finish between 1-5 keV. So I ask me myself sometimes if it's an "a priori" or a addiction ! While I say always to the students that they must try, to find the right energy for the right illustration of their observations.
But... in the daily work, our samples are most thin metallic films, oxyde nanoparticules, C nanotubes, etc, with the need of high magnifications. No mineralogy any more, nor metallography, only small thin light stuff which gives in most cases no usable pictures at 15 keV or more.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
DusevichV-at-umkc.edu a écrit : } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jacques, } } I cannot understand "addiction" to any kV range. In my work I routinely use all voltages my microscope can supply: from 300 V for easily damaged or charging specimens to 30 kV for "special effects". For example, at 30 kV I can get pictures of yeas colonies with white cells on black background (useful for analysis of shapes of colonies). At 15 kV I can make pictures with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20 kV could be useful in imaging fractures of multiphase specimens like composites and metals, when phases are highlighted. And so on. } } As for ESEM, low voltages ( {5kV for my microscope) are not really useful because of high noise level. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } Hi all } } } } In spite of beeing interesting by the concept of the ESEM } } since long ago, I had until now nor much time neither the } } real need to work on one. } } So I've a couple of questions about it. } } } } 1- First, most pictures done by ESEM in wet mode I've seen } } from collegues, either biologists or from material science, } } are done at high energy, 20-30 kV. It seems that the low } } energy "culture" is a bit ignored. (On the other hand, I } } agree that I'm perheps a bit addict to the low kV range !) } } But apart the subjecives aspects like "traditions", lab } } "culture" or the need of EDS, is there a more objective and } } technical reason for such choice ? Do the wet conditions } } limit so drastically the performences of the ESEM at low } } energy (lower then 5 keV } } f.ex.) ? } } } } 2- I a paper published in M&M 6-2000, Danilatos describes a } } very interesting evolution of the PLA (in particular for } } people interested in vaccum technology, like me...), called } } Reverse Flow Pressure Limiting Apperture. An annular gas jet } } flows from an intermediate stage at higher pressure into the } } specimen chamber, generating a pumping effect in the central } } part of the PLA and allowding to work with a greater } } differential pressure between the chamber and the column. } } I asked me if such a device has been soon integrated in } } commercial VP-SEMs, from FEI or other manufactuers. Danilatos } } mention too a cooperation with Zeiss on his website, but I } } never heard about a developpment like that by Zeiss. } } } } Thanks for comments } } } } Jacques } } } } -- } } J. Faerber } } IPCMS-GSI } } (Institut de Physique et Chimie des Matériaux de Strasbourg } } Groupe Surface et Interfaces) 23, rue de Loess ; BP43 } } 67034 Strasbourg CEDEX 2 } } France } } } } Tel 00 33(0)3 88 10 71 01 } } Fax 00 33(0)3 88 10 72 48 } } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } } } ==============================Original } } Headers============================== } } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24 } } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr } } (mailhost.u-strasbg.fr [130.79.200.157]) } } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n2O8ZMax011825 } } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar } } 2009 03:35:23 -0500 } } 9, 29 -- Received: from ipcms.u-strasbg.fr } } (ipcms.u-strasbg.fr [130.79.210.2]) } } 9, 29 -- by mailhost.u-strasbg.fr } } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280 } } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 } } Mar 2009 09:35:21 +0100 (CET) } } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr } } [130.79.152.3]) } } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA } } (256/256 bits)) } } 9, 29 -- (No client certificate requested) } } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id } } 5F52D3EC002 } } 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar } } 2009 09:34:14 +0100 (CET) } } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr} } } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From: } } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9, } } 29 -- MIME-Version: 1.0 9, 29 -- To: } } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain; } } charset=ISO-8859-1; format=flowed 9, 29 -- } } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: } } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: } } jacques.faerber-at-ipcms.u-strasbg.fr } } 9, 29 -- Subject: Quest. about the ESEM } } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by } } milter-greylist-4.0.1 (mailhost.u-strasbg.fr } } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9, } } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32 } } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 } } -- X-Spam-Status: No, score=-100.0 required=5.0 } } tests=USER_IN_WHITELIST } } 9, 29 -- autolearn=disabled version=3.2.5 } } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 } } (2008-06-10) on mr7.u-strasbg.fr } } ==============================End of - } } Headers============================== } } } } } } } } } ==============================Original Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009 } 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PG4PlI026286 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500 } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="iso-8859-1" } 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM } 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500 } 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM } 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA } 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 8, 25 -- To: {Microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC) FILETIME=[5D87D3C0:01C9AD63] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PG4PlI026286 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Mar 25 12:05:42 2009 9, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PH5fou009666 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:05:41 -0500 9, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n2PH5ct7066933 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 18:05:38 +0100 (CET) 9, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 31 -- (No client certificate requested) 9, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 3111C3EC001 9, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Mar 2009 18:04:25 +0100 (CET) 9, 31 -- Message-ID: {49CA6455.8040403-at-ipcms.u-strasbg.fr} 9, 31 -- Date: Wed, 25 Mar 2009 18:05:25 +0100 9, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 31 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318) 9, 31 -- MIME-Version: 1.0 9, 31 -- To: Microscopy-at-microscopy.com 9, 31 -- Subject: Re: [Microscopy] RE: Quest. about the ESEM 9, 31 -- References: {200903251609.n2PG9P5u001558-at-ns.microscopy.com} 9, 31 -- In-Reply-To: {200903251609.n2PG9P5u001558-at-ns.microscopy.com} 9, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-IPCMS-MailScanner: Found to be clean 9, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 9, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 25 Mar 2009 18:05:38 +0100 (CET) 9, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9165/Wed Mar 25 16:08:41 2009 on mr2.u-strasbg.fr 9, 31 -- X-Virus-Status: Clean 9, 31 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST 9, 31 -- autolearn=disabled version=3.2.5 9, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr2.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 22, 28 -- From protrain-at-emcourses.com Wed Mar 25 13:02:46 2009 22, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PI2h8V027035 22, 28 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 13:02:44 -0500 22, 28 -- Received: from 5ac8bf34.bb.sky.com ([90.200.191.52]:4885 helo=HP6220) 22, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 22, 28 -- (envelope-from {protrain-at-emcourses.com} ) 22, 28 -- id 1LmXQz-000OBX-Jj by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 25 Mar 2009 20:02:38 +0200 22, 28 -- Reply-To: {protrain-at-emcourses.com} 22, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 22, 28 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr} 22, 28 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 22, 28 -- References: {200903251708.n2PH80VM012463-at-ns.microscopy.com} 22, 28 -- Subject: Tradition or Science 22, 28 -- Date: Wed, 25 Mar 2009 18:02:36 -0000 22, 28 -- Organization: Protrain 22, 28 -- Message-ID: {001b01c9ad73$e2937a50$0200a8c0-at-HP6220} 22, 28 -- MIME-Version: 1.0 22, 28 -- Content-Type: text/plain; 22, 28 -- charset="iso-8859-1" 22, 28 -- X-Mailer: Microsoft Office Outlook 11 22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 22, 28 -- Thread-Index: AcmtbFDGBlAaAMq6QsSK8oTs72K/dgABixYg 22, 28 -- In-Reply-To: {200903251708.n2PH80VM012463-at-ns.microscopy.com} 22, 28 -- X-Scan-Signature: 99f4c94f445b5fbf97b5591e34ffbdcf{337}} 22, 28 -- X-Trace: smtp01.dial-up.net 1LmXQz-000OBX-Jj d3c9f4506833b893560218b1f3ba629a 22, 28 -- Content-Transfer-Encoding: 8bit 22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PI2h8V027035 ==============================End of - Headers==============================
I'm thinking on building a programmable slide stainer, starting from a liniar one. I own a Medite COT20 liniar stainer (see the leaflet -at- http://tinyurl.com/cgtxo5 or http://preview.tinyurl.com/cgtxo5). It's in working condition. It has no less than 27 stations, so it can be used to perform even rather demanding staining protocols such as those often used trichromes as AZAN, Van Gieson, Masson etc.
The working principle of liniar slide stainers is, that the slides are run trough the baths and the time in each bath is the same for all baths. That way it's possible to run basket after basket after basket... trough the machine, resulting in very high troughput numbers (the COT 20 should be capable of staining up to 1 000 slides/hour, according to Medite). Variation is done by altering the concentration of the solutions used or by putting several baths after each other.
An example. Let's say staining by hand: --------------------------------------- ... hematoxylin: 6 min differentiation 1% ²HCl in ETOH 70: 15 sec water: 2 min bluing: 2 min ...
would become something like this, using a liniar slide stainer: -------------------------------------------------------------- ... hematoxylin 1: 2 min hematoxylin 2: 2 min hematoxylin 3: 2 min differentiation 0.1% ²HCl in ETOH 70: 2 min water: 2 min bluing: 2 min ...
But protocols such as this example are impossible in a liniar slide stainer: ----------------------------------------------------------------------------
... mordanting 0.1% chromium trioxide in dH2O: 5h running water: 5h safranin 1% in ETOH 50: 20 min ETOH 50: 10 sec ...
For those you need a programmable slide stainer. The running water isn't the problem, it's the variation in treatment times.
As I also have a few old PC's gathering dust on my attic (486/33, 486/100, PI/166, PII/400...), I would like to use one of those to control the stainer using good ol' DOS batch files. I would prefer the 486/33 as it is a compact model with an integrated screen (one of those old Compaq Presario cubes).
The general idea is to control the stainer using an external relay board and a relay controller board in the computer. Given the age of the PC's I'm looking for a second hand ISA (prefeberaly) or a PCI controller board.
Controling the stainer shouldn't be that hard as about the only thing needed is a programmable timing cycle, a square wave with in time adjustable 1's and 0's. Or am I missing something? I've noticed already that it's rather simple to set up a timing cycle in NTFS-DOS by using ping 127.0.0.1 -n# but I suppose it isn't usable in DOS 6.**.
In the next couple of days I will dismantle the Cot 20 and draw it's wiring, but it looks like it's rather simple: the only thing needed is to control one cycle of a stepping motor in the machine. It's currently controlled by an Omron timer. There's no need to controll the other controls on the machine (draining time controlled by another Omron timer and agitation on/off) trough the PC.
Any thoughts?
Thanks in advance!
Y.
==============================Original Headers============================== 20, 22 -- From yvan_lindekens-at-yahoo.com Thu Mar 26 01:17:38 2009 20, 22 -- Received: from web110213.mail.gq1.yahoo.com (web110213.mail.gq1.yahoo.com [67.195.8.189]) 20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2Q6Hb7H006374 20, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 01:17:38 -0500 20, 22 -- Received: (qmail 88331 invoked by uid 60001); 26 Mar 2009 06:17:37 -0000 20, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238048257; bh=gBv/dIta4B/ojemlpbOVoQ2TxNPOIGs823zLvT+ZKZI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=1lrWCXfmjFIcJQ1lATl+M7hJWPp5L+WnRjIoumAX/GirM3Hy2xQQC2HEIWvOPLcHfrXw0TuWYBiwAhFERuDTsT7htYwuexDOUQRvwS7LDEQiNO57gjqAh1p9Zso8WEqGyohiss7oVCkwAZ2OGuLWUleAk+5lLL8qehvg0y5U6Pw= 20, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 22 -- s=s1024; d=yahoo.com; 20, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 20, 22 -- b=qZsyJZ12r+oaEgQPtSQ6l+lBU+SxauIyAI2ydmE8aPfD/s5y3l6zD1zh+pfxAhLKnSpAHUDFhExhmsRtaXMUhlNC4CxDIU294cpdW/n8RmxRPU5BxxZBXVzKxWu8AoXubE3KwMD/ob6wQCLIV/vZr5mP0wONIInB7t8734Zekpc=; 20, 22 -- Message-ID: {357798.87844.qm-at-web110213.mail.gq1.yahoo.com} 20, 22 -- X-YMail-OSG: HebBYGYVM1mr.f5zmvt5t8DV7OeBFw9hiwqP4ltsdyWk5XTV1olxls.y 20, 22 -- Received: from [87.64.45.30] by web110213.mail.gq1.yahoo.com via HTTP; Wed, 25 Mar 2009 23:17:36 PDT 20, 22 -- X-Mailer: YahooMailWebService/0.7.289.1 20, 22 -- Date: Wed, 25 Mar 2009 23:17:36 -0700 (PDT) 20, 22 -- From: yvan lindekens {yvan_lindekens-at-yahoo.com} 20, 22 -- Subject: LM: Homebrew programmable slide stainer: opinions and suggestions wanted 20, 22 -- To: Microscopy-at-microscopy.com 20, 22 -- MIME-Version: 1.0 20, 22 -- Content-Type: text/plain; charset=iso-8859-1 20, 22 -- Content-Transfer-Encoding: 8bit 20, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2Q6Hb7H006374 ==============================End of - Headers==============================
The most information or the most meaningful information?
I really think that the vast majority of SEM users know how to use the kV and why.
Now I wonder how long it took to take a picture at 100 V. Overnight? ;-)
Stephane
----- Original Message ---- X-from: "protrain-at-emcourses.com" {protrain-at-emcourses.com} To: nizets2-at-yahoo.com Sent: Wednesday, March 25, 2009 7:06:31 PM
Hi All
Taking up the comments about(E)SEM accelerating voltage and how it should be used I am often tempted to ask if a laboratory runs by tradition or by science, unfortunately too many run by tradition!
In my mind the most important question an SEM operator has to answer is "Which kV?" The correct answer in my mind is "The kV that displays the most information about the specimen." However I often find that to obtain the most information about a specimen it is good to view it at higher and lower accelerating voltages - my choice 2 & 10 or 5 and 15.
The only time I have my students use the highest accelerating voltages are when using BSE techniques. We have worked as low as 100 volts on a tungsten hairpin instrument, a 15 year old model in fact!
The motto "Microscopists are scientists they should experiment."
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: 25 March 2009 17:08 To: protrain-at-emcourses.com
Hi Vladimir
I agree fully with your comment. I said "addict" as a joke, as in our needs I'm so often in situation where I say to my collegues that we can try with higher kV, but that I "feel" that it will give nothing. And so it is, we try and finish between 1-5 keV. So I ask me myself sometimes if it's an "a priori" or a addiction ! While I say always to the students that they must try, to find the right energy for the right illustration of their observations.
But... in the daily work, our samples are most thin metallic films, oxyde nanoparticules, C nanotubes, etc, with the need of high magnifications. No mineralogy any more, nor metallography, only small thin light stuff which gives in most cases no usable pictures at 15 keV or more.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
DusevichV-at-umkc.edu a écrit : } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jacques, } } I cannot understand "addiction" to any kV range. In my work I routinely use all voltages my microscope can supply: from 300 V for easily damaged or charging specimens to 30 kV for "special effects". For example, at 30 kV I can get pictures of yeas colonies with white cells on black background (useful for analysis of shapes of colonies). At 15 kV I can make pictures with highlighted ("glowing") osteocytes in bone. Macrographs taken at 15-20 kV could be useful in imaging fractures of multiphase specimens like composites and metals, when phases are highlighted. And so on. } } As for ESEM, low voltages ( {5kV for my microscope) are not really useful because of high noise level. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } Hi all } } } } In spite of beeing interesting by the concept of the ESEM } } since long ago, I had until now nor much time neither the } } real need to work on one. } } So I've a couple of questions about it. } } } } 1- First, most pictures done by ESEM in wet mode I've seen } } from collegues, either biologists or from material science, } } are done at high energy, 20-30 kV. It seems that the low } } energy "culture" is a bit ignored. (On the other hand, I } } agree that I'm perheps a bit addict to the low kV range !) } } But apart the subjecives aspects like "traditions", lab } } "culture" or the need of EDS, is there a more objective and } } technical reason for such choice ? Do the wet conditions } } limit so drastically the performences of the ESEM at low } } energy (lower then 5 keV } } f.ex.) ? } } } } 2- I a paper published in M&M 6-2000, Danilatos describes a } } very interesting evolution of the PLA (in particular for } } people interested in vaccum technology, like me...), called } } Reverse Flow Pressure Limiting Apperture. An annular gas jet } } flows from an intermediate stage at higher pressure into the } } specimen chamber, generating a pumping effect in the central } } part of the PLA and allowding to work with a greater } } differential pressure between the chamber and the column. } } I asked me if such a device has been soon integrated in } } commercial VP-SEMs, from FEI or other manufactuers. Danilatos } } mention too a cooperation with Zeiss on his website, but I } } never heard about a developpment like that by Zeiss. } } } } Thanks for comments } } } } Jacques } } } } -- } } J. Faerber } } IPCMS-GSI } } (Institut de Physique et Chimie des Matériaux de Strasbourg } } Groupe Surface et Interfaces) 23, rue de Loess ; BP43 } } 67034 Strasbourg CEDEX 2 } } France } } } } Tel 00 33(0)3 88 10 71 01 } } Fax 00 33(0)3 88 10 72 48 } } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } } } ==============================Original } } Headers============================== } } 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 24 } } 03:35:23 2009 9, 29 -- Received: from mailhost.u-strasbg.fr } } (mailhost.u-strasbg.fr [130.79.200.157]) } } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n2O8ZMax011825 } } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar } } 2009 03:35:23 -0500 } } 9, 29 -- Received: from ipcms.u-strasbg.fr } } (ipcms.u-strasbg.fr [130.79.210.2]) } } 9, 29 -- by mailhost.u-strasbg.fr } } (8.14.2/jtpda-5.5pre1) with ESMTP id n2O8ZL9Z098280 } } 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 24 } } Mar 2009 09:35:21 +0100 (CET) } } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr } } [130.79.152.3]) } } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA } } (256/256 bits)) } } 9, 29 -- (No client certificate requested) } } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id } } 5F52D3EC002 } } 9, 29 -- for {microscopy-at-Microscopy.Com} ; Tue, 24 Mar } } 2009 09:34:14 +0100 (CET) } } 9, 29 -- Message-ID: {49C89B3C.2080108-at-ipcms.u-strasbg.fr} } } 9, 29 -- Date: Tue, 24 Mar 2009 09:35:08 +0100 9, 29 -- From: } } "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } } 9, 29 -- User-Agent: Thunderbird 2.0.0.19 (X11/20090105) 9, } } 29 -- MIME-Version: 1.0 9, 29 -- To: } } microscopy-at-microscopy.com 9, 29 -- Content-Type: text/plain; } } charset=ISO-8859-1; format=flowed 9, 29 -- } } Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: } } Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: } } jacques.faerber-at-ipcms.u-strasbg.fr } } 9, 29 -- Subject: Quest. about the ESEM } } 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by } } milter-greylist-4.0.1 (mailhost.u-strasbg.fr } } [130.79.200.157]); Tue, 24 Mar 2009 09:35:21 +0100 (CET) 9, } } 29 -- X-Virus-Scanned: ClamAV 0.94.2/9156/Tue Mar 24 05:11:32 } } 2009 on mr7.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 } } -- X-Spam-Status: No, score=-100.0 required=5.0 } } tests=USER_IN_WHITELIST } } 9, 29 -- autolearn=disabled version=3.2.5 } } 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 } } (2008-06-10) on mr7.u-strasbg.fr } } ==============================End of - } } Headers============================== } } } } } } } } } ==============================Original Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Wed Mar 25 11:04:26 2009 } 8, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PG4PlI026286 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 11:04:26 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Wed, 25 Mar 2009 11:04:23 -0500 } 8, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="iso-8859-1" } 8, 25 -- Subject: RE: [Microscopy] Quest. about the ESEM } 8, 25 -- Date: Wed, 25 Mar 2009 11:04:22 -0500 } 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB800-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200903240836.n2O8a718012704-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] Quest. about the ESEM } 8, 25 -- Thread-Index: AcmsW5RXzLt8I2j9R4yo7h5d7DUPKQBA+8QA } 8, 25 -- References: {200903240836.n2O8a718012704-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 8, 25 -- To: {Microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 25 Mar 2009 16:04:23.0932 (UTC) FILETIME=[5D87D3C0:01C9AD63] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PG4PlI026286 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Mar 25 12:05:42 2009 9, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PH5fou009666 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 12:05:41 -0500 9, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n2PH5ct7066933 9, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 18:05:38 +0100 (CET) 9, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 31 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 31 -- (No client certificate requested) 9, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 3111C3EC001 9, 31 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Mar 2009 18:04:25 +0100 (CET) 9, 31 -- Message-ID: {49CA6455.8040403-at-ipcms.u-strasbg.fr} 9, 31 -- Date: Wed, 25 Mar 2009 18:05:25 +0100 9, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 31 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318) 9, 31 -- MIME-Version: 1.0 9, 31 -- To: Microscopy-at-microscopy.com 9, 31 -- Subject: Re: [Microscopy] RE: Quest. about the ESEM 9, 31 -- References: {200903251609.n2PG9P5u001558-at-ns.microscopy.com} 9, 31 -- In-Reply-To: {200903251609.n2PG9P5u001558-at-ns.microscopy.com} 9, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-IPCMS-MailScanner: Found to be clean 9, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 9, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 25 Mar 2009 18:05:38 +0100 (CET) 9, 31 -- X-Virus-Scanned: ClamAV 0.94.2/9165/Wed Mar 25 16:08:41 2009 on mr2.u-strasbg.fr 9, 31 -- X-Virus-Status: Clean 9, 31 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST 9, 31 -- autolearn=disabled version=3.2.5 9, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr2.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 22, 28 -- From protrain-at-emcourses.com Wed Mar 25 13:02:46 2009 22, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2PI2h8V027035 22, 28 -- for {microscopy-at-microscopy.com} ; Wed, 25 Mar 2009 13:02:44 -0500 22, 28 -- Received: from 5ac8bf34.bb.sky.com ([90.200.191.52]:4885 helo=HP6220) 22, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 22, 28 -- (envelope-from {protrain-at-emcourses.com} ) 22, 28 -- id 1LmXQz-000OBX-Jj by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 25 Mar 2009 20:02:38 +0200 22, 28 -- Reply-To: {protrain-at-emcourses.com} 22, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 22, 28 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr} 22, 28 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 22, 28 -- References: {200903251708.n2PH80VM012463-at-ns.microscopy.com} 22, 28 -- Subject: Tradition or Science 22, 28 -- Date: Wed, 25 Mar 2009 18:02:36 -0000 22, 28 -- Organization: Protrain 22, 28 -- Message-ID: {001b01c9ad73$e2937a50$0200a8c0-at-HP6220} 22, 28 -- MIME-Version: 1.0 22, 28 -- Content-Type: text/plain; 22, 28 -- charset="iso-8859-1" 22, 28 -- X-Mailer: Microsoft Office Outlook 11 22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 22, 28 -- Thread-Index: AcmtbFDGBlAaAMq6QsSK8oTs72K/dgABixYg 22, 28 -- In-Reply-To: {200903251708.n2PH80VM012463-at-ns.microscopy.com} 22, 28 -- X-Scan-Signature: 99f4c94f445b5fbf97b5591e34ffbdcf{337}} 22, 28 -- X-Trace: smtp01.dial-up.net 1LmXQz-000OBX-Jj d3c9f4506833b893560218b1f3ba629a 22, 28 -- Content-Transfer-Encoding: 8bit 22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2PI2h8V027035 ==============================End of - Headers==============================
==============================Original Headers============================== 39, 24 -- From nizets2-at-yahoo.com Thu Mar 26 04:31:48 2009 39, 24 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237]) 39, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2Q9VlJC027468 39, 24 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 04:31:47 -0500 39, 24 -- Received: (qmail 18303 invoked by uid 60001); 26 Mar 2009 09:31:46 -0000 39, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238059906; bh=jvL67qBGgTkLlbjYpLm8dKQf9xS+u4ysbsgMqw+aalQ=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=u7XWzg8J/tLHafTbN1kS0p/8++IyTRkxJ7LntZ0w4V1eMZ3zbODHj3b44dOEXo3GvKfkzSb6w/JouNLFRVCXJgt7LxIOBYE9ZLJfgyGN5A3NzO3u2cwWQs+dM/6srll0enx9tOfvN5pGUVTYr+HdrW2J2Ki7qicsA4WiCEx6DuM= 39, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 39, 24 -- s=s1024; d=yahoo.com; 39, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 39, 24 -- b=3ifcXQzVU1DwEH6RW+Skso2L8z8ZutOCfLVfv9K25sRNgvoBVXa4ZXQdgq+VKBd8+yMHQ0zEZaysMexaNrW6PgB7gPsLE3psPaqIvGj4NN047vzBuiqc+SJ2dA+zzDLLmUjk8B8k1rwO9fCcHZX6daqCuRgmZWHQFBT6EfRdJ1E=; 39, 24 -- Message-ID: {219772.18074.qm-at-web110814.mail.gq1.yahoo.com} 39, 24 -- X-YMail-OSG: u0Qt7wIVM1lITpP0yGo0jlsCCEvenU4HtnelFz0uAdTuG3hFu_M2.TAZ 39, 24 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Thu, 26 Mar 2009 02:31:45 PDT 39, 24 -- X-Mailer: YahooMailRC/1277.32 YahooMailWebService/0.7.289.1 39, 24 -- References: {200903251806.n2PI6VFb006201-at-ns.microscopy.com} 39, 24 -- Date: Thu, 26 Mar 2009 02:31:45 -0700 (PDT) 39, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com} 39, 24 -- Subject: Re: [Microscopy] Tradition or Science 39, 24 -- To: microscopy-at-microscopy.com 39, 24 -- In-Reply-To: {200903251806.n2PI6VFb006201-at-ns.microscopy.com} 39, 24 -- MIME-Version: 1.0 39, 24 -- Content-Type: text/plain; charset=iso-8859-1 39, 24 -- Content-Transfer-Encoding: 8bit 39, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2Q9VlJC027468 ==============================End of - Headers==============================
"TEM micrographs are not what the student would like to present in his paper".
I am shocked. Perhaps it would be the right moment to teach the student that his method is not a scientific one but more like a sectarian one. A scientist doesn't choose the technique and then looks for a way to reach his goal with this technique. A scientist actually has a goal and chooses the technique(s) which is(are) the most adapted to reach his goal.
I think for this case Electrontomography could give interesting results. If he still refuses to even try that, you can still suggest that he tries politics. In politics, whatever the method, the most important thing is to reach the goal (*)
Best regards,
Stephane
(*) Please don't send me insulting emails. This was just intended to be humour. No personal harm intended. Not even to politicans. I swear. In case of offense, just replace "politics" by "professional sport".
----- Original Message ---- X-from: "dhorne-at-interchange.ubc.ca" {dhorne-at-interchange.ubc.ca} To: nizets2-at-yahoo.com Sent: Tuesday, March 24, 2009 6:16:46 PM
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Question: We have a student interested in looking at the morphologies (fibre arrangement and spacing) of fibrin clots treated with various stabilizing agents as a bulk sample, not as a thin layer. I have tried a few different techniques- CPD vs HMDS, ROTO, holding the clot between dialysis membranes in a cartridge arrangement (for the record this was a miserable failure),and processing the clot formed in an Eppendorf tube- but continue to have issues with collapse of the clot.
We've done a lit search but haven't been able to find a definitive technique for a bulk clot. It is important to note this is an SEM application, and that TEM micrographs are not what the student would like to present in his paper.
I'm considering taking the clots to 100% EtOH and then freeze-drying, but my gut tells me the clots will still collapse.
I am wondering if it is even possible to keep a bulk clot expanded? Should fibrin be thought of as a cable, without strength in compression? If so, how have people worked around this in the past?
About a year ago I installed a new bulb that registered as having 400 hours on it fresh out of the box.
Last night I was about to start a timelapse and the Exfo light went out and the display on the unit indicated over one thousand hours (it actually had much less than that) and gave a light error.
This morning I replaced the bulb with a fresh one from a sealed box and the Exfo unit started the bulb fine but the display indicates the new bulb has 641 hours on it.
Taken together these three events suggest to me that the problem is with the unit itself and not the bulbs. Has anybody else seen a problem like this with the X-Cite 120?
Mike
Michael J. Herron, U of MN, Dept. of Entomology herro001-at-umn.edu 612-624-3688 (office) 612-625-5299 (FAX)
==============================Original Headers============================== 10, 18 -- From herro001-at-umn.edu Thu Mar 26 09:32:37 2009 10, 18 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232]) 10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2QEWaXQ000327 10, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 09:32:37 -0500 10, 18 -- Received: from [134.84.48.158] (x84-48-158.cfans.umn.edu [134.84.48.158]) 10, 18 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP 10, 18 -- Thu, 26 Mar 2009 09:32:34 -0500 (CDT) 10, 18 -- X-Umn-Remote-Mta: [N] x84-48-158.cfans.umn.edu [134.84.48.158] #+LO+TS+AU+HN 10, 18 -- X-Umn-Classification: local 10, 18 -- Mime-Version: 1.0 (Apple Message framework v753.1) 10, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 10, 18 -- Message-Id: {003C070D-010E-4F9B-9985-24E10221E8FF-at-umn.edu} 10, 18 -- Content-Transfer-Encoding: 7bit 10, 18 -- From: Michael Herron {herro001-at-umn.edu} 10, 18 -- Subject: Exfo X-cite 120 problem 10, 18 -- Date: Thu, 26 Mar 2009 09:32:27 -0500 10, 18 -- To: Microscopy-at-microscopy.com 10, 18 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
I made for you couple stereo pairs of TEM copper grid at x50 and displacement (translation) of 500 um. First pair taken at tilt angle 0 (not much of topography, but you can measure parameters of grid from them). Second pair taken at tilt 30, so you have a lot of height difference between top and bottom of images. Displacement and tilt axis were along horizontal axis of images.
Could you explain me practical purpose of your software? Is it intended as low magnification supplement for in-lens SEM? From my estimate, parallax we have to measure is roughly equal to (t*h)/w, where h - height, t - translation, w - working distance. Suppose we acquire pictures 1000*1000 pixels wit w=10 mm. At magnification x100 field of view is about 1000 um and we have 1 um/pixel. At highest practical values of measured height h and translation t equal to half of field of view value, i.e. 500 um, parallax will be 25 um (25 pixels). Not too bad. But if we want to measure feature which height is 10 times lower (5% of field of view), then we have just 2-3 pixels of parallax, so an error of measurements will be about 50%. At magnification x1000 we should expect 50% error even for height of ½ of field of view - too high for any practical purposes. Of coarse, acquiring pictures 4000*4000 pixels will decrease error four times, and lower w, as in in-lens SEM, will improve things also.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Ahmad; Your detector window is most likely MOXTEK, an aluminum oxide/polymer multilayer composite supported by a silicon grid. You can read about it at: www.moxtek.com/PDF/Windows/AP3%20Window.pdf
John Mardinly, Numonyx
-----Original Message----- X-from: ahmad_ds-at-yahoo.com [mailto:ahmad_ds-at-yahoo.com] Sent: Wednesday, March 25, 2009 5:25 AM To: MARDINLY, A
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Email: ahmad_ds-at-yahoo.com Name: Ahmad Ashkhaibi
Organization: Al-Balqa Applied University
Title-Subject: [Filtered] EDS window
Question: I work on and EDS manufactured by EDAX of the model XL-30 SUTW. I want to know what is the material of fabrication of the super ultra-thin window of the EDS I have.
Lee worked in the microscopy field from 1968 to 2004, developing instrumentation, and training hundreds of students.
Leroy Louis Dreyer (Lee) Sept. 18, 1925 - Mar 18, 2009
Lee Dreyer, 83, passed away peacefully, in his wife's arms in Stockton, CA, on Mar 18, 2009 after a courageous 14 month battle with cancer. He was born on Sept 18, 1925 in St. Louis, MO, a birthday he shared with his late mother, Olga Goedecke, of St. Louis. He was also preceded in death by: his father & stepmother, Louis & Marie Dreyer, of St. Louis; and his son, David Dreyer of Champaign, IL. He is survived by his beloved family and friends which include: his wife, Judy Murphy (Stockton, CA); sister, Dolores Kornfeld (Buffalo, NY); 3 daughters - Sandy & Nat Simpkins(Manchester, MA) - Karen & Bob Graham and Joni & Brad Hendricks, all of Champaign, IL; 5 grandchildren - Chris Nelson (New Hampshire), David Hartley (Bolivia, SA), and Davena, Bobby & Michael Graham (Champaign, IL); 5 great-grandchildren - Adam & Zoe Nelson (New Hampshire), Andrea & David John Hartley (Bolivia, SA), and Sydnee Graham (North Carolina); and 2 nieces - Suzanne and Marilyn Kornfeld and 1 nephew - Robert Kornfeld.
EDUCATION: Lee graduated from Hadley Vocational Technical School (St. Louis) specializing in Electrical & Radio Repair in 1942. He joined the Army Signal Corps (Rolla, MO) in Communication and Pre-radar followed by enlisting in the Navy in 1943. He attended US Navy Service Schools in Airborne Communication & Radar and served in the Asian/Pacific in World War 2. He was an Aviation Electronic Technician Mate, First Class, and did aircraft maintenance on the Marianas Islands of Tinian, Saipan, and Guam. After the war, Lee received his Assoc. of Arts in Pre-engineering at Harris Teachers Col, St. Louis, and his BS in Electrical Engineering at the University of Ill, Urbana, IL in 1956. In 1984, Lee received certification in Digital & Microprocessor Electronics at Missouri Tech. Sch., St. Louis.
WORK EXPERIENCE: From 1947-52, Lee worked as an Engineer at Western Electric, Emerson Electric & Vickers Electric, all in St. Louis. University of Illinois (Urbana,IL): 1952-68, Bio-Physics Lab, and the Gaseous Electronics Lab as Electronics Engineer; 1968-71, Center for Electron Microscopy as EM Engineer/Instructor. Southern Illinois University: 1971-83 (Carbondale), Center for Electron Microscopy as EM Engineer/Instructor; 1986-1992 (Edwardsville), School of Science as Electronics Engineer. 1994-2004, San Joaquin Delta College (Stockton, CA), Microscopy Technology Center in Microscopy Instruction.
HONORS: Lee is co-inventor and has several patents (1952-62) as well as articles in scientific journals with Drs. William and Frank Fry, & Dr. Russell Meyers, on ultrasonic irradiation in hyperkinetic and hypertonic disorders (Parkinson's Disease).
Lee truly enjoyed vocational education. He was always involved in training students to use instrumentation but came into his own when training researchers/students in electron microscopy. He thoroughly enjoyed watching his students blossom as they mastered the instruments. He had the patience of Job during his one on one sessions. His students loved him and appreciated his patience, expert help and compassion as well as his unique sense of humor.
Lee also enjoyed developing instrumentation for various projects. He always said that he fixed everything Judy broke!!!!!
HOBBIES: Lee enjoyed fishing, boating, scuba diving, and traveling. Lee and his wife Judy, traveled throughout their 39 yrs together, circumnavigating the globe, and living life to its fullest.
His heart, his kindness, his honesty, his incredible sense of humor, and his compassion touched all who knew him. Lee will be in our hearts and minds forever. He was loved by all and will be dearly missed. He truly does walk with angels now.
SERVICES: Funeral Home: Kutis Funeral Home, 10151 Gravois Rd, Affton, MO 63123 (314/842-4458). Visitation: April 2, 2009: 3-9 pm Chapel Service (at Kutis): April 3, 2009: 11:30 am Interment: April 3, 2009 (after chapel service) Jefferson Barracks National Cemetery, 2900 Sheridan Rd., St. Louis, MO 63125 In lieu of flowers, donations can be made to the Lee Dreyer Memorial Fund. A memorial garden will be made at his home. Send donations to Lee Dreyer Memorial Fund, PMB# 119, 4719 Quail Lakes Dr., Ste G, Stockton, CA 95207-5267.
==============================Original Headers============================== 13, 20 -- From murphyjudy-at-comcast.net Thu Mar 26 16:13:13 2009 13, 20 -- Received: from QMTA12.emeryville.ca.mail.comcast.net (qmta12.emeryville.ca.mail.comcast.net [76.96.27.227]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2QLDDoq024647 13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 16:13:13 -0500 13, 20 -- Received: from OMTA01.emeryville.ca.mail.comcast.net ([76.96.30.11]) 13, 20 -- by QMTA12.emeryville.ca.mail.comcast.net with comcast 13, 20 -- id XsPK1b01t0EPchoACxDEj5; Thu, 26 Mar 2009 21:13:14 +0000 13, 20 -- Received: from [192.168.1.3] ([76.127.99.233]) 13, 20 -- by OMTA01.emeryville.ca.mail.comcast.net with comcast 13, 20 -- id XxDD1b00K527pzg8MxDDnt; Thu, 26 Mar 2009 21:13:14 +0000 13, 20 -- Message-ID: {49CBEFEA.4050004-at-comcast.net} 13, 20 -- Date: Thu, 26 Mar 2009 14:13:14 -0700 13, 20 -- From: Judy Murphy {murphyjudy-at-comcast.net} 13, 20 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 13, 20 -- X-Accept-Language: en-us, en 13, 20 -- MIME-Version: 1.0 13, 20 -- To: Microscopy {Microscopy-at-microscopy.com} 13, 20 -- Subject: Lee Dreyer Walks With Angels, Service Info 13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Klugem07-at-bu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Klugem07-at-bu.edu Name: Matt
Organization: Boston University Medical Center
Title-Subject: [Filtered] EM Image Calibration
Question: Recently, I have been tracing EM images of organelles in white cells, using ImageJ.
I need help calibrating my images, because it is becoming clear that merely using the scale provided in the image is not accutate.
Currently, I am analyzing images at two different magnification settings.
With the basic calibration method I have been using, It seems that the organelle areas are approximately half as large at a direct magnification of 30000x and 59900 x 8.0in print magnification as they are at 12000x magnification and 177000 x 8.0in print magnification.
I have spoken to someone at our Medical Center who suggested a simple print mag formula, Print mag = length of structure on print / actual length.
As I have already mentioned, one of the values I am obtaining is area, but I'm afraid that this formula will not help me for a 2-D measurement.
Hello Matt, When I started measuring carbon black by TEM, to assure myself of the calibration, I printed images of my calibration grid at the magnifications I was interested in and chech them witha ruler and calculator. I also reran those images with my on-line measuring program to confirm the electronic measurements. As a last step I ran spheres from Duke Scientific to assure myself that both the system and I was performing the measurements correctly.
stay safe......... Frank
Klugem07-at-bu.edu
03/26/2009 08:25 To PM frank_karl-at-lincolnelectric.com cc
Please respond to Subject Klugem07-at-bu.edu [Microscopy] viaWWW: EM Image Calibration
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Klugem07-at-bu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Klugem07-at-bu.edu Name: Matt
Organization: Boston University Medical Center
Title-Subject: [Filtered] EM Image Calibration
Question: Recently, I have been tracing EM images of organelles in white cells, using ImageJ.
I need help calibrating my images, because it is becoming clear that merely using the scale provided in the image is not accutate.
Currently, I am analyzing images at two different magnification settings.
With the basic calibration method I have been using, It seems that the organelle areas are approximately half as large at a direct magnification of 30000x and 59900 x 8.0in print magnification as they are at 12000x magnification and 177000 x 8.0in print magnification.
I have spoken to someone at our Medical Center who suggested a simple print mag formula, Print mag = length of structure on print / actual length.
As I have already mentioned, one of the values I am obtaining is area, but I'm afraid that this formula will not help me for a 2-D measurement.
==============================Original Headers============================== 14, 11 -- From zaluzec-at-microscopy.com Thu Mar 26 19:18:07 2009 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2R0I6NV010946 14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 26 Mar 2009 19:18:06 -0500 14, 11 -- Mime-Version: 1.0 14, 11 -- Message-Id: {p06240800c5f1cbac95de-at-[206.69.208.22]} 14, 11 -- Date: Thu, 26 Mar 2009 19:18:04 -0500 14, 11 -- To: microscopy-at-microscopy.com 14, 11 -- From: Klugem07-at-bu.edu (by way of MicroscopyListserver) 14, 11 -- Subject: viaWWW: EM Image Calibration 14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 31, 22 -- From frank_karl-at-lincolnelectric.com Fri Mar 27 05:41:34 2009 31, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 31, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RAfYXS013205 31, 22 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 05:41:34 -0500 31, 22 -- In-Reply-To: {200903270025.n2R0PMZF023384-at-ns.microscopy.com} 31, 22 -- Subject: Re: [Microscopy] viaWWW: EM Image Calibration 31, 22 -- To: Klugem07-at-bu.edu, Microscopy-at-microscopy.com 31, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 31, 22 -- Message-ID: {OF12DECAFE.78888A27-ON85257586.003A36FB-85257586.003AB5CA-at-lincolnelectric.com} 31, 22 -- Date: Fri, 27 Mar 2009 06:41:11 -0400 31, 22 -- From: Frank_Karl-at-lincolnelectric.com 31, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 31, 22 -- 07, 2008) at 03/27/2009 06:41:10 AM, 31, 22 -- CD-MIME complete at 03/27/2009 06:41:10 AM, 31, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 31, 22 -- 07, 2008) at 03/27/2009 06:41:10 AM, 31, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 31, 22 -- 07, 2008) at 03/27/2009 06:41:10 AM, 31, 22 -- Serialize complete at 03/27/2009 06:41:10 AM 31, 22 -- MIME-Version: 1.0 31, 22 -- Content-Type: text/plain; 31, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
Your figures suggest that a 30,000x picture enlarged is 59,900x but your 12,000x picture enlarged is 177,000x. I assume there is a zero missing or added somewhere or I've misunderstood.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Klugem07-at-bu.edu
Hello, Following error appeared two times in the last two days on our Philips CM12 OPCON display: "ERROR MESSAGE: SOLID-STATE-KEY". After that, the microscope did not response to any key (button) with exception of RESTART and OFF. The user manual states: Contact service department. Does anybody has experiences with this error? Thanks for any suggestions.
With best regards Oldrich
-- Oldřich Benada Institute of Microbiology, Acad. Sci. CR, v.v.i. Videnska 1083 142 20 Prague 4 - Krc Czech Republic
==============================Original Headers============================== 4, 23 -- From benada-at-biomed.cas.cz Fri Mar 27 08:53:45 2009 4, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RDrj73014355 4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 08:53:45 -0500 4, 23 -- Received: from u117ob.mbu.cas.cz (u117ob.mbu.cas.cz [147.231.44.101]) 4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 23 -- (No client certificate requested) 4, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 0910A1A445A5 4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 14:53:44 +0100 (CET) 4, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 23 -- Reply-To: =?utf-8?q?Old=C5=99ich_Benada?= {benada-at-biomed.cas.cz} 4, 23 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?=, v.v.i. 4, 23 -- To: microscopy-at-microscopy.com 4, 23 -- Subject: Philips CM12 trouble 4, 23 -- Date: Fri, 27 Mar 2009 14:53:04 +0100 4, 23 -- User-Agent: KMail/1.9.9 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: text/plain; 4, 23 -- charset="utf-8" 4, 23 -- Content-Disposition: inline 4, 23 -- Message-Id: {200903271453.04622.benada-at-biomed.cas.cz} 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RDrj73014355 ==============================End of - Headers==============================
I am not sure of your procedure. However, the magnification formula is correct.
Here's how I always calculated and converted between micrometer distances and mags.
General Rule Procedure: Take the magnification and convert it to KX. Let's say you get 50 KX. That number in millimeters is the length of ONE micron at that mag of 50 KX. So in this example, 50 mm is one micrometer (or 5 mm is 0.1 µm). This mag rule works for TEM, SEM, OM, et al.
You could try to draw a calibration bar 50 mm long and label it as 1 µm, but the print is not that wide. So use one-tenth of that millimeter distance and and label it as 0.1µm. Always determine the one micron distance first and then convert up or down from there. This procedure can be used in the reverse order and is the more common procedure.
Here's a few example of how to use this procedure or rule. Let's say you see an image in Microscopy Today that shows an internal calibration bar in the image and it says 0.1 µm. So what's the magnification in MT? Did the author know how much his image would be reduced or enlarged? No. Say the 0.1 µm bar is 6 millimeters long. Convert that to one micron and you get 60 millimeters for one micrometer. The mag of the image in MT is 60 KX regardless of what the labeling under the image says or the article.
Here's an optical micrograph calculation example. Your print was determined by a stage micrometer calibration to be 37 X. That's 0.037 KX. So one micron is 0.037 mm but you can't draw that length. So use 3.7 mm and label the bar as 100 microns.
To calibrate an optical microscope, you need a calibration standard called a stage micrometer. Mag*I*Cal is a calibration standard for TEM but most microscopists still use the common carbon grating replica (cheaper). Beautifully made NIST traceable step standards can be purchased from VLSI (vlsistandards.com) for all types of measurements. We used one for Mirau interferometry. The LVSI standards are not cheap but they are certified traceable and VLSI is ISO9001 certified.
For image analysis, you need to photograph a standard and "pull" it into your CRT with your software. Then you can calibrate your software directly to your known distance standard.
This mag rule is very helpful and easy to use "on the fly". I gave a presentation with a 35 mm slide of a TEM micrograph displayed it onto a large projector screen. Some guy asked, "What is the actual mag on the screen?" I picked up a meter stick, measured the distance for the 0.1 µm calibration bar as 150 mm, multiplied by ten to get the one micron distance, multiplied by 1000, and I said, "Close to 1.5 million X."
Paul Beauregard
At 07:18 PM 3/26/09 -0500, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 12, 29 -- From beaurega-at-westol.com Fri Mar 27 09:54:25 2009 12, 29 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2REsORt030548 12, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 09:54:24 -0500 12, 29 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 12, 29 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n2REsB3e032178 12, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 10:54:13 -0400 12, 29 -- Received: (qmail 8710 invoked by uid 89); 27 Mar 2009 14:54:07 -0000 12, 29 -- Received: from pitts-69-72-13-253.dynamic-dialup.coretel.net (HELO running) (69.72.13.253) 12, 29 -- by mail.winbeam.com with SMTP; 27 Mar 2009 14:54:07 -0000 12, 29 -- Message-Id: {3.0.6.32.20090327115417.00857740-at-pop3.norton.antivirus} 12, 29 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 12, 29 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 12, 29 -- Date: Fri, 27 Mar 2009 11:54:17 -0500 12, 29 -- To: Klugem07-at-bu.edu, microscopy-at-microscopy.com 12, 29 -- From: Beaurega {beaurega-at-westol.com} 12, 29 -- Subject: Re: [Microscopy] viaWWW: EM Image Calibration 12, 29 -- In-Reply-To: {200903270018.n2R0IarF011566-at-ns.microscopy.com} 12, 29 -- Mime-Version: 1.0 12, 29 -- Content-Type: text/plain; charset="iso-8859-1" 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 12, 29 -- X-Winbeam-MailScanner-ID: n2REsB3e032178 12, 29 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 12, 29 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 12, 29 -- SpamAssassin (not cached, score=-0.882, required 4, AWL -0.69, 12, 29 -- BAYES_00 -2.00, SARE_SPEC_REPLICA_OBFU 1.81) 12, 29 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com 12, 29 -- X-Winbeam-MailScanner-Watermark: 1238770453.86976-at-k8OsoFJYgNAWQ3IGJNOluA ==============================End of - Headers==============================
We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).
I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.
But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"
Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for). Link to image (I have a few more images): http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg
I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.
Thanks!
Geoff Williams Leduc Bioimaging Facility Manager Brown University
I just wanted to follow up and say I was there overseeing the CPD of these cells and I'm very confident in this particular process. It is a Ladd unit (Polaron) and I've been using these with great success since '92 or so. Maybe '93? (Long ago enough that I don't think one or two years makes much of a difference).
I observed the CPD run this time and it couldn't have been better from a temp/pressure/flush/vent process.
(Yes, I should have added this the first time - sorry).
Geoff Williams Leduc Bioimaging Facility Manager Brown University
-----Original Message----- X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu] Sent: Friday, March 27, 2009 12:12 PM To: Williams, Geoffrey
We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).
I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.
But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"
Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for). Link to image (I have a few more images): http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg
I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.
Thanks!
Geoff Williams Leduc Bioimaging Facility Manager Brown University
I've seen this lots of time from when I was doing cells. Any or all of fixation, dehydration, and CPD could cause this. Also post-CPD handling, meaning, are they wearing gloves? Are they keeping the samples in containers in desiccators and never leaving them sit outside? Fixation: Are they using 1% monomeric tannic acid in the glut and maybe also in the osmium? The glut should be 1%, maybe 1.25%, no more. Fix for one hour, 2 at most. Dehydration: Spread cells on substrate dehydrate quickly, so 3 or 5 minutes per step is enough, 50-70-80-90-95-3X100% EtOH. Might have to start at 30% EtOH or even lower. Some folks will do 5% steps. CPD: Most CPD manufacturers use the wrong steps for drying. The lqCO2 has to be infiltrated and exchanged for the EtOH in the same way the EtOH has to be exchanged for water in the cells. Use X changes for Y minutes each, depending on samples. For cells on impermeable substrates (metal, glass), I used 3 changes ("soaks") for 5 minutes each. Depending on how many samples and how packed they were. Another possible source of the problem -- the lqCO2 and EtOH have to flow freely around the samples to properly exchange, otherwise EtOH is left behind. Make sure to purge well between lqCO2 changes -- 2-3 minutes, usually. /And/ be sure to lower the pressure SLOWLY once the critical point is exceeded. "Too slowly" can't happen. Mind, the cracks being mostlly in the thin, spread regions do make me think they could be due to incomplete dehydration, or extraction during dehydration. But, the problem could come from any of the 3 main steps above. What is the method they used?
Phil
} We have someone here who is trying to image } cells grown on Titanium squares, both flat and } micro-structured surfaces. They were getting } decent results at one point and lately, or } really the last month or two they have been } chasing problems (as I understand the situation). } } I've looked over the protocol and it seems, to } me, to be correct. The samples are chemically } fixed (buffered Glute and OsO4), dehydrated } (ETOH), CPD, coated and imaged. } } But without going into more details I'm just } wondering if someone out here might be able to } look at the image and say "Oh yeah, I know } exactly what the problem is and how to fix it!" } } Hopefully though that solution doesn't involve } high vacuum freeze drying or cryo-sem (neither } of which we have the equipment for). } Link to image (I have a few more images): } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg } } I am not personally doing the prep, but have } been asked to see what can be done to help, esp } since they are most interested in keeping the } cellular projections intact. It may be a very } simple issue but honestly maybe I've had too } much botanical training in terms of sample prep } and am missing it. } } Thanks! } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } Ý } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 28 -- From oshel1pe-at-cmich.edu Fri Mar 27 12:37:50 2009 5, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RHbnWm007188 5, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 12:37:50 -0500 5, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 28 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n2RHbht3018150; 5, 28 -- Fri, 27 Mar 2009 13:37:43 -0400 5, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 5, 28 -- Fri, 27 Mar 2009 13:37:28 -0400 5, 28 -- Mime-Version: 1.0 5, 28 -- Message-Id: {f0624080bc5f2b941cfd9-at-[141.209.160.249]} 5, 28 -- In-Reply-To: {200903271612.n2RGCaAI020365-at-ns.microscopy.com} 5, 28 -- References: {200903271612.n2RGCaAI020365-at-ns.microscopy.com} 5, 28 -- Date: Fri, 27 Mar 2009 13:37:25 -0400 5, 28 -- To: Geoffrey_Williams-at-brown.edu 5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 28 -- Subject: Re: [Microscopy] SEM artifact help wanted 5, 28 -- Cc: Microscopy-at-microscopy.com 5, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 5, 28 -- X-OriginalArrivalTime: 27 Mar 2009 17:37:28.0715 (UTC) FILETIME=[B32595B0:01C9AF02] 5, 28 -- X-Canit-CHI2: 0.00 5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 28 -- X-Spam-Score: -2.60 () [Hold at 5.00] L_EXCH_MF,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 5, 28 -- X-CanItPRO-Stream: default 5, 28 -- X-Canit-Stats-ID: 10887683 - d0d6b0e5f639 5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RHbnWm007188 ==============================End of - Headers==============================
I agree with Phil - there are many factors, But the one that made the most difference for me recently was to do the dehydration in much smaller steps, starting with 10%. And I know Phil has shown that soaks in the lqCO2 really help!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
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Geoff I have often seen this artifact on cultured cells processed for SEM. I believe the problem may be due to allowing the cells to 'dry out' between changes of the higher percentages (95 and 100) of ETOH or when the cells are transferred to the CPD chamber. It only take a couple of seconds for the thin layer of ETOH to evaporate off the cells leading to this nicely crackled surface.
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu] Sent: Friday, March 27, 2009 12:17 PM To: rjharris-at-uwo.ca
We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).
I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.
But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"
Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for). Link to image (I have a few more images): http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.j pg
I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.
Thanks!
Geoff Williams Leduc Bioimaging Facility Manager Brown University
Dear Geoff Your preparation looks fine to me so far I can zoom in your picture, and I am trying to think: The various podia projecting from one side of each cell are seen attached to the floor and detached from the cell's main -and nice- body that probably overlies nucleus. At the opposite -the ugly- side the plasma membrane is more spread like a leaf as opposed to the thread like projections of the other side. It is the leaf side I suspect people do not like. But animal cells shrink a lot during dehydration and drying while we mummify them for SEM. More than a dried leaf. The cells of the picture look strongly attached to the substratum and shrinkage damaged them in both sides, the podia were broken, the leaf-like membrane was fragmented. This is how it happens when you prepare animal cells on solid substrates for SEM, you subject them big forces like cell to substratum adherence versus cellular integrity. I don't understand why your titanum client is not happy with these results; running controls like plastic could help him aqcuiring a more complete image of what he expects to see. Good luck! yorgos
eikonika-at-otenet.gr yorgosnikas-at-hotmail.com Tel/fax +30 210 8957677 Mobile +30 6945 107477
----- Original Message ----- X-from: {Geoffrey_Williams-at-brown.edu} To: {eikonika-at-otenet.gr} Sent: Friday, March 27, 2009 7:44 PM
HI Geoff,
I notice you did not do the preparation. Are you sure the individuals who did are competent? There are lots of little nuances for each of several steps any one of which could result in trouble. Are they aware of and understand this or just cook booking through some protocol someone gave them- the gist of which looks sound by the way. Split prep in this case could be to your benefit allowing them to look over their protocols as opposed to anything you are doing on CPD
I have to agree with some of the other posts- this looks an awful lot like drying artifact but could easily go all the way back to growth conditions. Are they sure the absolute alcohol is absolute? Water exerts a tremendous amount of force and these cells seem to be pulling away from the substrate. Sieve with cupric sulfate indicator will prevent hydroscopic absorption. Mine needs recharging every 6 months or so in humidity and temp controlled museum- like clockwork 8 years now so I know something is up if it goes early. Same goes for the CO2 tanks. 99% of the time there is no need for the drying sieve but that 1% is still out there. I once ended up with the wrong grade CO2 and if not running through sieve would likely have contained traces of water blowing the samples. I now read the delivery ticket a little more carefully but nothing to say it couldn't happen again. Are they using different sample holders changing infiltration properties as ethanol is not very miscible with lCO2.
Even though you charge your users it might make sense for them to give you a set to prep yourself independently of them running the same set with the so called tried and true protocol and see what happens. If the expert is getting good cells to begin with then they will come out as such. Both of you come out with garbage...
Having seen posts from you in the past I suspected you covered the bases, but I decided this was a good opportunity for discussion and enlightenment at all experience levels. Troubleshooting tried and true protocols or methods can be a very time-consuming and frustrating experience if not properly prepared. In a case such as this I would concentrate most of my effort on what has changed since the last successful run, in particular things that could cause the cells to shrink- and no detail is too minute. A well run facility makes this easier as they mitigate change or uncertainty wherever possible. For instance where possible I try never to completely run out of any material or chemical and will begin using a new batch prior to the old running out. It's amazingly easy in a busy lab to move a decimal point and suddenly that 1x buffer is 10x. Something goes wrong and you have a more limited list of potential candidates and time to re-order or switch suppliers, which of course you have previously tried in the past and know will work equally well. It is not uncommon to order chemistry etc. which while having the same catalog number has changed manufacturers, materials, processing or whatever. I seem to recall an issue with glutaraldehyde recently on this list, and how many times have I seen Kodak cursed on this list over the years. Thankfully as an all digital facility I escaped those but it just served to reinforce maintenance of good lab management protocols and fallbacks.
Good luck,
Scott Whittaker Head NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
-----Original Message----- X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu] Sent: Friday, March 27, 2009 12:09 PM To: Whittaker, Scott
We have someone here who is trying to image cells grown on Titanium squares, both flat and micro-structured surfaces. They were getting decent results at one point and lately, or really the last month or two they have been chasing problems (as I understand the situation).
I've looked over the protocol and it seems, to me, to be correct. The samples are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated and imaged.
But without going into more details I'm just wondering if someone out here might be able to look at the image and say "Oh yeah, I know exactly what the problem is and how to fix it!"
Hopefully though that solution doesn't involve high vacuum freeze drying or cryo-sem (neither of which we have the equipment for). Link to image (I have a few more images): http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg
I am not personally doing the prep, but have been asked to see what can be done to help, esp since they are most interested in keeping the cellular projections intact. It may be a very simple issue but honestly maybe I've had too much botanical training in terms of sample prep and am missing it.
Thanks!
Geoff Williams Leduc Bioimaging Facility Manager Brown University
This may be a different angle... There is a greater expansion of metal than there is of the biological material, cells, when the sample is taken from Liquid CO2 temp through the heated part of the CPD cycle past the critical temperature. Could HMDS be a reasonable alternative to CPD?
A control could also be growing the same type cells on a glass coverslip to be run through the CPD. A comparison would show only a difference of the substrate. Titanium squares vs. glass coverslips.
Regards, Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
=== } From: {WHITTAKS-at-si.edu} } Reply-To: {WHITTAKS-at-si.edu} } Date: Fri, 27 Mar 2009 15:14:46 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] RE: SEM artifact help wanted } } HI Geoff, } } I notice you did not do the preparation. Are you sure the individuals who did } are competent? There are lots of little nuances for each of several steps any } one of which could result in trouble. Are they aware of and understand this or } just cook booking through some protocol someone gave them- the gist of which } looks sound by the way. Split prep in this case could be to your benefit } allowing them to look over their protocols as opposed to anything you are } doing on CPD } } I have to agree with some of the other posts- this looks an awful lot like } drying artifact but could easily go all the way back to growth conditions. Are } they sure the absolute alcohol is absolute? Water exerts a tremendous amount } of force and these cells seem to be pulling away from the substrate. Sieve } with cupric sulfate indicator will prevent hydroscopic absorption. Mine needs } recharging every 6 months or so in humidity and temp controlled museum- like } clockwork 8 years now so I know something is up if it goes early. Same goes } for the CO2 tanks. 99% of the time there is no need for the drying sieve but } that 1% is still out there. I once ended up with the wrong grade CO2 and if } not running through sieve would likely have contained traces of water blowing } the samples. I now read the delivery ticket a little more carefully but } nothing to say it couldn't happen again. Are they using different sample } holders changing infiltration properties as ethanol is not very miscible wit! } h lCO2. } } Even though you charge your users it might make sense for them to give you a } set to prep yourself independently of them running the same set with the so } called tried and true protocol and see what happens. If the expert is getting } good cells to begin with then they will come out as such. Both of you come out } with garbage... } } Having seen posts from you in the past I suspected you covered the bases, but } I decided this was a good opportunity for discussion and enlightenment at all } experience levels. Troubleshooting tried and true protocols or methods can be } a very time-consuming and frustrating experience if not properly prepared. In } a case such as this I would concentrate most of my effort on what has changed } since the last successful run, in particular things that could cause the cells } to shrink- and no detail is too minute. A well run facility makes this easier } as they mitigate change or uncertainty wherever possible. For instance where } possible I try never to completely run out of any material or chemical and } will begin using a new batch prior to the old running out. It's amazingly easy } in a busy lab to move a decimal point and suddenly that 1x buffer is 10x. } Something goes wrong and you have a more limited list of potential candidates } and time to re-order or switch suppliers, which of course y! } ou have previously tried in the past and know will work equally well. It is } not uncommon to order chemistry etc. which while having the same catalog } number has changed manufacturers, materials, processing or whatever. I seem to } recall an issue with glutaraldehyde recently on this list, and how many times } have I seen Kodak cursed on this list over the years. Thankfully as an all } digital facility I escaped those but it just served to reinforce maintenance } of good lab management protocols and fallbacks. } } Good luck, } } } Scott Whittaker } Head NMNH Imaging } Manager SEM Lab } Smithsonian Institution } National Museum of Natural History } PO Box 37012 MRC104 } Washington DC 20013-7012 } 202-633-0891 } } } -----Original Message----- } X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu] } Sent: Friday, March 27, 2009 12:09 PM } To: Whittaker, Scott } Subject: [Microscopy] SEM artifact help wanted ------------------------------------------------------------------------ } } We have someone here who is trying to image cells grown on Titanium squares, } both flat and micro-structured surfaces. They were getting decent results at } one point and lately, or really the last month or two they have been chasing } problems (as I understand the situation). } } I've looked over the protocol and it seems, to me, to be correct. The samples } are chemically fixed (buffered Glute and OsO4), dehydrated (ETOH), CPD, coated } and imaged. } } But without going into more details I'm just wondering if someone out here } might be able to look at the image and say "Oh yeah, I know exactly what the } problem is and how to fix it!" } } Hopefully though that solution doesn't involve high vacuum freeze drying or } cryo-sem (neither of which we have the equipment for). } Link to image (I have a few more images): } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_cell_problem.jpg } } I am not personally doing the prep, but have been asked to see what can be } done to help, esp since they are most interested in keeping the cellular } projections intact. It may be a very simple issue but honestly maybe I've had } too much botanical training in terms of sample prep and am missing it. } } Thanks! } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ } }
} ==============================Original Headers============================== } 24, 29 -- From WHITTAKS-at-si.edu Fri Mar 27 15:10:59 2009 } 24, 29 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu } [160.111.103.177]) } 24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n2RKAw3i007479 } 24, 29 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:10:58 -0500 } 24, 29 -- Received: from si-msesmtpo-01.US.SINET.SI.EDU } (SI-MSESMTP-N1.us.sinet.si.edu [160.111.49.75]) } 24, 29 -- by si-mailout03.si.edu (Postfix) with ESMTP id 48CE16AC3; } 24, 29 -- Fri, 27 Mar 2009 16:10:54 -0400 (EDT) } 24, 29 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by } si-msesmtpo-01.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959); } 24, 29 -- Fri, 27 Mar 2009 16:10:54 -0400 } 24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 24, 29 -- Content-class: urn:content-classes:message } 24, 29 -- MIME-Version: 1.0 } 24, 29 -- Content-Type: text/plain; } 24, 29 -- charset="iso-8859-1" } 24, 29 -- Subject: RE: [Microscopy] SEM artifact help wanted } 24, 29 -- Date: Fri, 27 Mar 2009 16:10:52 -0400 } 24, 29 -- Message-ID: } {20CDD1CED2E76541A4FA119C6C806BA30395467F-at-SI-ECL02.US.SINET.SI.EDU} } 24, 29 -- In-Reply-To: {200903271608.n2RG8v3m016738-at-ns.microscopy.com} } 24, 29 -- X-MS-Has-Attach: } 24, 29 -- X-MS-TNEF-Correlator: } 24, 29 -- Thread-Topic: [Microscopy] SEM artifact help wanted } 24, 29 -- Thread-Index: Acmu9leksGOaLsPSRCu4+GLAcLfdZQAD7XNQ } 24, 29 -- References: {200903271608.n2RG8v3m016738-at-ns.microscopy.com} } 24, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} } 24, 29 -- To: {microscopy-at-microscopy.com} } 24, 29 -- Cc: {Geoffrey_Williams-at-brown.edu} } 24, 29 -- X-OriginalArrivalTime: 27 Mar 2009 20:10:54.0056 (UTC) } FILETIME=[21F52680:01C9AF18] } 24, 29 -- Content-Transfer-Encoding: 8bit } 24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n2RKAw3i007479 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 27 -- From connellyps-at-nhlbi.nih.gov Fri Mar 27 15:42:01 2009 9, 27 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RKfxES022249 9, 27 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:41:59 -0500 9, 27 -- X-IronPortListener: Outbound_SMTP 9, 27 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 9, 27 -- by nihxway2out.hub.nih.gov with ESMTP; 27 Mar 2009 16:41:58 -0400 9, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 27 -- Fri, 27 Mar 2009 16:41:54 -0400 9, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.162]) with Microsoft Exchange Server HTTP-DAV ; 9, 27 -- Fri, 27 Mar 2009 20:41:53 +0000 9, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 27 -- Date: Fri, 27 Mar 2009 16:40:31 -0400 9, 27 -- Subject: Re: [Microscopy] RE: SEM artifact help wanted 9, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 27 -- To: {WHITTAKS-at-si.edu} 9, 27 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 27 -- Message-ID: {C5F2B1FF.332A%connellyps-at-nhlbi.nih.gov} 9, 27 -- Thread-Topic: [Microscopy] RE: SEM artifact help wanted 9, 27 -- Thread-Index: AcmvHEUZg9tsRxsPEd6fYwANk2Yv1A== 9, 27 -- In-Reply-To: {200903272014.n2RKEkTJ014900-at-ns.microscopy.com} 9, 27 -- Mime-version: 1.0 9, 27 -- Content-type: text/plain; 9, 27 -- charset="ISO-8859-1" 9, 27 -- X-OriginalArrivalTime: 27 Mar 2009 20:41:54.0241 (UTC) FILETIME=[76B6DB10:01C9AF1C] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RKfxES022249 ==============================End of - Headers==============================
X-from time to time I am getting cell cultures of the same cell line, prepared by different people (graduate students, post docs.) Quality of specimens depends very much on a preparer. You can ask your researcher if somebody new in a lab prepared these cultures. The most common mistake is to let cells dry between steps of protocol. Keeping things simple, like dropping OsO4 (it is not really needed, glut alone is good enough for SEM) and switching from CPD to HMDS could help obtain more reproducible results.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: Geoffrey_Williams-at-brown.edu } [mailto:Geoffrey_Williams-at-brown.edu] } Sent: Friday, March 27, 2009 11:08 AM } To: Dusevich, Vladimir } Subject: [Microscopy] SEM artifact help wanted } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } We have someone here who is trying to image cells grown on } Titanium squares, both flat and micro-structured surfaces. } They were getting decent results at one point and lately, or } really the last month or two they have been chasing problems } (as I understand the situation). } } I've looked over the protocol and it seems, to me, to be } correct. The samples are chemically fixed (buffered Glute } and OsO4), dehydrated (ETOH), CPD, coated and imaged. } } But without going into more details I'm just wondering if } someone out here might be able to look at the image and say } "Oh yeah, I know exactly what the problem is and how to fix it!" } } Hopefully though that solution doesn't involve high vacuum } freeze drying or cryo-sem (neither of which we have the } equipment for). } Link to image (I have a few more images): } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/SEM_ } cell_problem.jpg } } I am not personally doing the prep, but have been asked to } see what can be done to help, esp since they are most } interested in keeping the cellular projections intact. It } may be a very simple issue but honestly maybe I've had too } much botanical training in terms of sample prep and am missing it. } } Thanks! } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ } } } } ==============================Original } Headers============================== } 9, 28 -- From Geoffrey_Williams-at-brown.edu Fri Mar 27 11:06:28 } 2009 9, 28 -- Received: from honeydew.services.brown.edu } (honeydew.services.brown.edu [128.148.106.194]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n2RG6RN8014388 } 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar } 2009 11:06:28 -0500 } 9, 28 -- Received: from ex-gateway2-out.AD.Brown.Edu } (ex-gateway2.ad.brown.edu [128.148.21.53]) } 9, 28 -- by honeydew.services.brown.edu (8.14.3/8.14.3) } with ESMTP id n2RG6PC6031327 } 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Mar } 2009 12:06:26 -0400 } 9, 28 -- Received: from mail1.AD.Brown.Edu ([128.148.21.35]) } by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.3959); } 9, 28 -- Fri, 27 Mar 2009 12:06:26 -0400 } 9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 28 } -- Content-class: urn:content-classes:message 9, 28 -- } MIME-Version: 1.0 9, 28 -- Content-Type: text/plain; } 9, 28 -- charset="iso-8859-1" } 9, 28 -- Subject: SEM artifact help wanted 9, 28 -- Date: } Fri, 27 Mar 2009 12:06:26 -0400 9, 28 -- Message-ID: } {A1A84D541C161C4C988B4E0FB38F5A0F0792D956-at-MAIL1.AD.Brown.Edu} } 9, 28 -- X-MS-Has-Attach: } 9, 28 -- X-MS-TNEF-Correlator: } 9, 28 -- Thread-Topic: SEM artifact help wanted 9, 28 -- } Thread-Index: Acmu9fspX+09lVnQR+KKHpx2uSHELA== 9, 28 -- From: } "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} 9, 28 -- } To: {microscopy-at-microscopy.com} 9, 28 -- } X-OriginalArrivalTime: 27 Mar 2009 16:06:26.0696 (UTC) } FILETIME=[FB87A080:01C9AEF5] 9, 28 -- } X-Proofpoint-Virus-Version: vendor=nai engine=5.3.00 } definitions=5565 signatures=515473 9, 28 -- } X-Proofpoint-Spam-Details: rule=quarantine_notspam } policy=quarantine score=0 spamscore=0 ipscore=0 phishscore=0 } bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx } engine=5.0.0-0811170000 definitions=main-0903270105 9, 28 -- } Content-Transfer-Encoding: 8bit 9, 28 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n2RG6RN8014388 } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 25 -- From DusevichV-at-umkc.edu Fri Mar 27 15:49:18 2009 7, 25 -- Received: from KC-MSXPROTO2.kc.umkc.edu (kc-msxproto2.kc.umkc.edu [134.193.143.155]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2RKnHLb003455 7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Mar 2009 15:49:18 -0500 7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by KC-MSXPROTO2.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 25 -- Fri, 27 Mar 2009 15:49:15 -0500 7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 25 -- Content-class: urn:content-classes:message 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="iso-8859-1" 7, 25 -- Subject: RE: [Microscopy] SEM artifact help wanted 7, 25 -- Date: Fri, 27 Mar 2009 15:49:14 -0500 7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB80A-at-KC-MSX1.kc.umkc.edu} 7, 25 -- In-Reply-To: {200903271607.n2RG7awX015281-at-ns.microscopy.com} 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- Thread-Topic: [Microscopy] SEM artifact help wanted 7, 25 -- Thread-Index: Acmu9iZMZ576iX44TtSANclCodHjigAJz57g 7, 25 -- References: {200903271607.n2RG7awX015281-at-ns.microscopy.com} 7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 25 -- To: {Geoffrey_Williams-at-brown.edu} , {Microscopy-at-microscopy.com} 7, 25 -- X-OriginalArrivalTime: 27 Mar 2009 20:49:15.0372 (UTC) FILETIME=[7DA61AC0:01C9AF1D] 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2RKnHLb003455 ==============================End of - Headers==============================
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Question: Hello. I have inherited an Ultracut microtome from another lab. It is missing the manual. Does anyone have a pdf or hard copy they could send me?
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Email: Lindsay.P.Keller-at-nasa.gov Name: Lindsay P. Keller
Organization: NASA
Title-Subject: [Filtered] Job opening: Electron Microscopy Scientist
Question: Electron Microscopy Scientist Description The Astromaterials Research and Exploration Science Directorate (ARES) at NASA's Johnson Space Center (JSC) is seeking a team member with expertise in Scanning Electron Microscopy (SEM) to support ongoing research and analysis projects. ARES is responsible for the curation and analysis of a wide range of solar system materials including the Apollo lunar samples, a large meteorite collection, cosmic & comet dust samples (Stardust mission samples), samples from the Sun (Genesis sample return mission), and space-exposed hardware. Ongoing research includes a wide range of planetary, meteoritical, and space exploration topics. ARES staff member backgrounds include geology, chemistry, astronomy, physics, plus biology, mathematics, computer science, and engineering.
We are searching for an individual with broad experience in scanning electron microscopy to assist and train researchers and students in the use of a field-emission SEM and a low-vacuum SEM instrument. Typical analyses include secondary electron imaging, backscattered electron imaging, low voltage work, and X-ray analysis and mapping using energy-dispersive x-ray spectrometry. Responsibilities include, but are not limited to: maintaining and operating all aspects of these microscopes, coordinating service for the instruments, and supporting peer-review research through high quality analyses of astromaterials. Secondary responsibilities may include development of new analytical techniques. In addition to the SEMs, the facility also houses state-of-the art transmission electron microscopes, and electron microprobe, and supporting instrumentation.
A bachelor's degree from an accredited university is a minimum requirement; the degree should be in an applicable geoscience, materials science, or engineering field. An advanced degree with strong experience in one of these fields is highly preferred. Five years of relevant experience is preferred. The candidate should have a strong grasp of the theory and practice of scanning electron microscopy analysis and data reduction. Experience with field emission sources is a plus. Knowledge of geological and planetary mineralogy is a plus, as is the ability to interpret analyses in a geologically meaningful way. Good computer skills are essential. Because the employee will often be working with students, and others who may be unfamiliar with electron beam instruments, good people skills are required. Must meet eligibility requirements to receive and maintain a DoD security clearance (i.e., almost certainly needs to be a US citizen).
For more information, please contact:
Lindsay P. Keller Manager, Electron Beam Analysis Labs Mail Code KR NASA Johnson Space Center Houston, TX 77058 Lindsay.P.Keller-at-nasa.gov
Do you use WATERFREE CO2 for CPD-drying?? the standard carbon dioxide contains to my knowledge (at leat over here in germany) considerable amount of water after switching to (very expensive and certified ) water-free CO2 we never again saw these cracks and ruptures in cell cuture cells in the last 25 years. In my experience the problem with water-free-CO2 never posed in tissue or other "solid" organs...it seems to me, that (single) cell culture cells are especially sensitive to even lowest content of water during CPD as said in another posting drying of 100% ethanol (or in my case, acetone) over molecular sieve helps a lot to avoid embedding or drying problems. good luck, peter
Is there a documented rule of thumb regarding minimum resolution per pixel when measuring particles of a certain size range in an image? For example, if one were interested in measuring particles down to 6 microns would one set magnification to yield 3 microns per pixel or 2 microns per pixel in the acquired image? (or less?)
Again, looking for documentation on this. I need to demonstrate proper resolution to peers in Europe and it is not enough that I just "say so" without any supportive data.
Thank you for any help or pointers.
Regards, Jackie
Jacqueline Ayotte Microscopist - Advanced Materials Characterization Ticona 8040 Dixie Highway Florence KY 41042 859-372-3139 fax 859-372-3184 jacqueline.ayotte-at-ticona.com The information contained in this e-mail, and any attachments thereto, is confidential and is intended only for use by the individual(s) and/or entity named above. If you are not the intended recipient of this e-mail, you are hereby notified that any dissemination, distribution or copying of this communication or any disclosure of the contents of this communication to others is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this e-mail. Please then delete the original including all attachments and any copy of any e-mail and printout thereof.
==============================Original Headers============================== 11, 27 -- From Jacqueline.Ayotte-at-ticona.com Mon Mar 30 08:35:44 2009 11, 27 -- Received: from exprod7og114.obsmtp.com (exprod7og114.obsmtp.com [64.18.2.215]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2UDZfAj023794 11, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 08:35:43 -0500 11, 27 -- Received: from source ([148.163.79.14]) by exprod7ob114.postini.com ([64.18.6.12]) with SMTP 11, 27 -- ID DSNKSdDKqlMnO5ROJzAcKdFGlbXDxz0tn+QU-at-postini.com; Mon, 30 Mar 2009 06:35:43 PDT 11, 27 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by smtp1.celanese.com with Microsoft SMTPSVC(6.0.3790.3959); 11, 27 -- Mon, 30 Mar 2009 08:35:38 -0500 11, 27 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959); 11, 27 -- Mon, 30 Mar 2009 08:35:37 -0500 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- Subject: OM - resolution rule of thumb? 11, 27 -- Date: Mon, 30 Mar 2009 09:35:35 -0400 11, 27 -- Message-ID: {A2D45D1AE135A5418A4D38183241688F031C55-at-amfloxmbx1.SW.CZDS.BZ} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: OM - resolution rule of thumb? 11, 27 -- thread-index: AcmxPGgKG21fsMlsS5umd5lrybpNhA== 11, 27 -- From: "Ayotte, Jacqueline M., Ticona/US" {Jacqueline.Ayotte-at-ticona.com} 11, 27 -- To: {Microscopy-at-microscopy.com} 11, 27 -- X-OriginalArrivalTime: 30 Mar 2009 13:35:37.0995 (UTC) FILETIME=[6952ADB0:01C9B13C] 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2UDZfAj023794 ==============================End of - Headers==============================
ASTM has a standard on computerized imaging system for counting carbon black particles with a TEM. The standard (sorry I don't have the vol or standard no.) recommends resolution per pixel related to magnification. These values were based on a 1 meg camera.
hope this helps.. Frank
Jacqueline.Ayotte -at-ticona.com To 03/30/2009 09:59 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] OM - resolution rule Jacqueline.Ayotte of thumb? -at-ticona.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello All,
Is there a documented rule of thumb regarding minimum resolution per pixel when measuring particles of a certain size range in an image? For example, if one were interested in measuring particles down to 6 microns would one set magnification to yield 3 microns per pixel or 2 microns per pixel in the acquired image? (or less?)
Again, looking for documentation on this. I need to demonstrate proper resolution to peers in Europe and it is not enough that I just "say so" without any supportive data.
Thank you for any help or pointers.
Regards, Jackie
Jacqueline Ayotte Microscopist - Advanced Materials Characterization Ticona 8040 Dixie Highway Florence KY 41042 859-372-3139 fax 859-372-3184 jacqueline.ayotte-at-ticona.com The information contained in this e-mail, and any attachments thereto, is confidential and is intended only for use by the individual(s) and/or entity named above. If you are not the intended recipient of this e-mail, you are hereby notified that any dissemination, distribution or copying of this communication or any disclosure of the contents of this communication to others is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this e-mail. Please then delete the original including all attachments and any copy of any e-mail and printout thereof.
==============================Original Headers============================== 11, 27 -- From Jacqueline.Ayotte-at-ticona.com Mon Mar 30 08:35:44 2009 11, 27 -- Received: from exprod7og114.obsmtp.com (exprod7og114.obsmtp.com [64.18.2.215]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2UDZfAj023794 11, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 08:35:43 -0500 11, 27 -- Received: from source ([148.163.79.14]) by exprod7ob114.postini.com ([64.18.6.12]) with SMTP 11, 27 -- ID DSNKSdDKqlMnO5ROJzAcKdFGlbXDxz0tn+QU-at-postini.com; Mon, 30 Mar 2009 06:35:43 PDT 11, 27 -- Received: from amalnxowa1.SW.CZDS.BZ ([148.163.84.25]) by smtp1.celanese.com with Microsoft SMTPSVC(6.0.3790.3959); 11, 27 -- Mon, 30 Mar 2009 08:35:38 -0500 11, 27 -- Received: from amfloxmbx1.SW.CZDS.BZ ([148.163.118.111]) by amalnxowa1.SW.CZDS.BZ with Microsoft SMTPSVC(6.0.3790.3959); 11, 27 -- Mon, 30 Mar 2009 08:35:37 -0500 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- Subject: OM - resolution rule of thumb? 11, 27 -- Date: Mon, 30 Mar 2009 09:35:35 -0400 11, 27 -- Message-ID: {A2D45D1AE135A5418A4D38183241688F031C55-at-amfloxmbx1.SW.CZDS.BZ} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: OM - resolution rule of thumb? 11, 27 -- thread-index: AcmxPGgKG21fsMlsS5umd5lrybpNhA== 11, 27 -- From: "Ayotte, Jacqueline M., Ticona/US" {Jacqueline.Ayotte-at-ticona.com} 11, 27 -- To: {Microscopy-at-microscopy.com} 11, 27 -- X-OriginalArrivalTime: 30 Mar 2009 13:35:37.0995 (UTC) FILETIME= [6952ADB0:01C9B13C] 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2UDZfAj023794 ==============================End of - Headers==============================
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==============================Original Headers============================== 30, 22 -- From frank_karl-at-lincolnelectric.com Mon Mar 30 09:34:16 2009 30, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 30, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2UEYGRq007609 30, 22 -- for {microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 09:34:16 -0500 30, 22 -- In-Reply-To: {200903301359.n2UDxmhZ005041-at-ns.microscopy.com} 30, 22 -- Subject: Re: [Microscopy] OM - resolution rule of thumb? 30, 22 -- To: Jacqueline.Ayotte-at-ticona.com, Microscopy-at-microscopy.com 30, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 30, 22 -- Message-ID: {OF121A7C77.B405EEAB-ON85257589.004F5FFD-85257589.005001F7-at-lincolnelectric.com} 30, 22 -- Date: Mon, 30 Mar 2009 10:33:58 -0400 30, 22 -- From: Frank_Karl-at-lincolnelectric.com 30, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 30, 22 -- 07, 2008) at 03/30/2009 10:33:56 AM, 30, 22 -- CD-MIME complete at 03/30/2009 10:33:56 AM, 30, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 30, 22 -- 07, 2008) at 03/30/2009 10:33:56 AM, 30, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 30, 22 -- 07, 2008) at 03/30/2009 10:33:56 AM, 30, 22 -- Serialize complete at 03/30/2009 10:33:56 AM 30, 22 -- MIME-Version: 1.0 30, 22 -- Content-Type: text/plain; 30, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
Location: Duke University Medical Center, Durham, NC
Requirements: BS degree. US citizen or green card. Prior training and experience in running electron microscopes, proficiency in cutting ultrathin sections and performing negative staining. Knowledge of scientific laboratory operation (making solutions, ordering supplies, typing results, keeping records, etc.). Clinical laboratory and research experience is advantageous.
Laboratory description: The work force consists of the director and 6 EM technologists who perform pathology (500 samples/year), virology (1000 samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes?2 with cryo attachments, plus ancillary specimen preparation equipment.
EM Laboratory web site: http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain
Send resume to: Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Phone: 919 684-3452 Fax: 919 684-3265 Email: saram-at-duke.edu
==============================Original Headers============================== 9, 26 -- From hale0007-at-mc.duke.edu Mon Mar 30 13:42:56 2009 9, 26 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2UIgt03031599 9, 26 -- for {Microscopy-at-microscopy.org} ; Mon, 30 Mar 2009 13:42:56 -0500 9, 26 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54]) 9, 26 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n2UIgpP92248860 9, 26 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO) 9, 26 -- for {Microscopy-at-microscopy.org} ; Mon, 30 Mar 2009 14:42:52 -0400 9, 26 -- Importance: Normal 9, 26 -- X-Priority: 3 (Normal) 9, 26 -- In-Reply-To: 9, 26 -- References: 9, 26 -- Subject: EM Tech Position Available 9, 26 -- MIME-Version: 1.0 9, 26 -- From: Michael J Hale {hale0007-at-mc.duke.edu} 9, 26 -- To: Microscopy-at-microscopy.org 9, 26 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release 9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 14:42:47, 9, 26 -- MIME-CD complete at 03/30/2009 14:42:48, 9, 26 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release 9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 02:42:51 PM 9, 26 -- Message-ID: {OFAE1A9166.74E35CBD-ON85257589.0066CB8E-85257589.0066CBF5-at-notes.duke.edu} 9, 26 -- Date: Mon, 30 Mar 2009 14:42:48 -0400 9, 26 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008 9, 26 -- Content-type: text/plain; charset=US-ASCII 9, 26 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201 ==============================End of - Headers==============================
Location: Duke University Medical Center, Durham, NC
Requirements: BS degree. US citizen or green card. Prior training and experience in running electron microscopes, proficiency in cutting ultrathin sections and performing negative staining. Knowledge of scientific laboratory operation (making solutions, ordering supplies, typing results, keeping records, etc.). Clinical laboratory and research experience is advantageous.
Laboratory description: The work force consists of the director and 6 EM technologists who perform pathology (500 samples/year), virology (1000 samples/year), and research work, 3 TEMs, 1 SEM, 7 ultramicrotomes?2 with cryo attachments, plus ancillary specimen preparation equipment.
EM Laboratory web site: http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain
Send resume to: Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710 Phone: 919 684-3452 Fax: 919 684-3265 Email: saram-at-duke.edu
==============================Original Headers============================== 9, 26 -- From hale0007-at-mc.duke.edu Mon Mar 30 13:48:00 2009 9, 26 -- Received: from porthos.duhs.duke.edu (porthos.duhs.duke.edu [152.16.199.201]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2UIlwhw004946 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 13:47:59 -0500 9, 26 -- Received: from notesgv.notes.duke.edu (notesgv.notes.duke.edu [152.16.18.54]) 9, 26 -- by porthos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id n2UIlumC995436 9, 26 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO) 9, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Mar 2009 14:47:56 -0400 9, 26 -- Importance: Normal 9, 26 -- X-Priority: 3 (Normal) 9, 26 -- In-Reply-To: 9, 26 -- References: 9, 26 -- Subject: EM Tech Position Available 9, 26 -- MIME-Version: 1.0 9, 26 -- From: Michael J Hale {hale0007-at-mc.duke.edu} 9, 26 -- To: Microscopy-at-microscopy.com 9, 26 -- X-MIMETrack: MIME-CD by Notes Server on bombadil2.notes.duke.edu/DUMC_Services/mc/Duke(Release 9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 14:47:51, 9, 26 -- MIME-CD complete at 03/30/2009 14:47:51, 9, 26 -- Serialize by Router on notesgv.notes.duke.edu/DUMC_Services/mc/Duke(Release 9, 26 -- 8.0.2|August 07, 2008) at 03/30/2009 02:47:55 PM 9, 26 -- Message-ID: {OFE9DBC44C.036B659D-ON85257589.00674231-85257589.0067423D-at-notes.duke.edu} 9, 26 -- Date: Mon, 30 Mar 2009 14:47:51 -0400 9, 26 -- X-Mailer: Lotus Domino Web Server Release 8.0.2 August 07, 2008 9, 26 -- Content-type: text/plain; charset=US-ASCII 9, 26 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.199.201 ==============================End of - Headers==============================
Dear all, Thank you for all your comments, either positive or negative. They sure help me in reshaping the site. I am sorry that I rushed my previous messages in trying to get a quick response to your comments. They were done in a Chinese internet bar (with a beer and many kids playing games around me) when I was on vacation in China. If they sound like unprofessional and too informal, I am sorry about that. Now about me: Shixin Wang BS in Physics at Beijing Polytechnic University, Beijing, China, 1986. Ph.D in Earth and Planetary Science at University of New Mexico, 1997. Post-doc fellow on Radiation effect and TEM at University of Michigan, 1997-2000. Senior Engineer, Micron Technology Inc., Boise, Idaho, 2000 - present.
I started on TEM training on 1992 when I started at Univ of New Mexico. and used TEM and related techniques ever since. Now in TEM laboratory at Micron Technology for 9 years, I consider myself a TEM professional.
I started this site: http://www.emfocal.com about a month ago. I registered a company "Emfocal Inc" for this site. Reason? (1) Separating myself from any possible legal issues; (2) Making it a non-personal site; and (3) It can be passed over to other generations.
The primary function of our site is an on-line publication platform. There are wiki-books, forums, article, FAQ, Image gallery, resume, etc. It is a public service site. I do reserve the right to put some advertisements on the site to gain income. The site is user interactive and dynamic, you can post, modify, or delete you post. When the site grows, some users can become site moderators or administrators.
It is hosted at hostmonster.com. It is a cheap hosting, but offers unlimited storage use. So far, it suits my needs. When the site grows in traffic, it may need to be moved to a dedicated (more expensive) server.
The site is not redundant as to this listserver. For one thing, there are wiki-book and article publications. If you have lecture notes, tutorials, you may want to publish them there to benefit more people. That site will not prevent you to publish your work again at other place. After I get clearance from my employer, I will post some of my stuff on. If you want to post resume or job posting specifically for the EM field, that is a place to go.
The "terms of use" was not clear enough for professional use. I will rewrite it and make it simple.
Your comments and participations are always welcome.
Thanks,
Sam
==============================Original Headers============================== 11, 31 -- From samwarren-at-emfocal.com Mon Mar 30 15:20:05 2009 11, 31 -- Received: from outbound-mail-358.bluehost.com (outbound-mail-358.bluehost.com [66.147.249.252]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2UKK5DL029980 11, 31 -- for {Microscopy-at-Microscopy.Com} ; Mon, 30 Mar 2009 15:20:05 -0500 11, 31 -- Received: (qmail 18071 invoked by uid 0); 30 Mar 2009 20:14:55 -0000 11, 31 -- Received: from unknown (HELO host361.hostmonster.com) (66.147.240.161) 11, 31 -- by outboundproxy7.bluehost.com.bluehost.com with SMTP; 30 Mar 2009 20:14:55 -0000 11, 31 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=emfocal.com; 11, 31 -- h=Message-ID:Date:Subject:From:To:User-Agent:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:Importance:X-Identified-User; 11, 31 -- b=ayWqzUzPbbxlXwf/pcc65zcyY5ygSsCbaE6oNj2eVDGPZ4Y+CGbv6LUt+Rm0zCCe4kgZfjAfwZpl3LSJ66s3I/L47+Mmi2tVM1JQ6DPzv4CN5NEM9iLNAwW+smbgmKwT; 11, 31 -- Received: from localhost ([127.0.0.1] helo=host361.hostmonster.com) 11, 31 -- by host361.hostmonster.com with esmtpa (Exim 4.69) 11, 31 -- (envelope-from {samwarren-at-emfocal.com} ) 11, 31 -- id 1LoNxi-0007qs-2H 11, 31 -- for Microscopy-at-Microscopy.Com; Mon, 30 Mar 2009 14:20:02 -0600 11, 31 -- Received: from 208.100.250.244 ([208.100.250.244]) 11, 31 -- (SquirrelMail authenticated user samwarren-at-emfocal.com) 11, 31 -- by host361.hostmonster.com with HTTP; 11, 31 -- Mon, 30 Mar 2009 14:20:02 -0600 (MDT) 11, 31 -- Message-ID: {2565.208.100.250.244.1238444402.squirrel-at-host361.hostmonster.com} 11, 31 -- Date: Mon, 30 Mar 2009 14:20:02 -0600 (MDT) 11, 31 -- Subject: TEM - New info about emfocal.com 11, 31 -- From: samwarren-at-emfocal.com 11, 31 -- To: Microscopy-at-Microscopy.Com 11, 31 -- User-Agent: SquirrelMail/1.4.13 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Type: text/plain;charset=iso-8859-1 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-Priority: 3 (Normal) 11, 31 -- Importance: Normal 11, 31 -- X-Identified-User: {2151:host361.hostmonster.com:emfocalc:emfocal.com} {sentby:program running on server} ==============================End of - Headers==============================
I need help in analyzing 200-300nm liposomes using TEM and SEM. What's the best way to prepare a suspension of liposomes for size analysis? Any suggestions/recommendations would be appreciated.
==============================Original Headers============================== 2, 23 -- From osborndc-at-umsl.edu Mon Mar 30 19:31:24 2009 2, 23 -- Received: from stl-proto10.umsl.edu (STL-PROTO10.umsl.edu [134.124.45.28]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V0VNJt019267 2, 23 -- for {Microscopy-at-Microscopy.com} ; Mon, 30 Mar 2009 19:31:24 -0500 2, 23 -- Received: from stl-mail4.stl.umsl.edu ([134.124.45.88]) by stl-proto10.umsl.edu with Microsoft SMTPSVC(6.0.3790.3959); 2, 23 -- Mon, 30 Mar 2009 19:31:20 -0500 2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 23 -- Content-class: urn:content-classes:message 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Type: text/plain; 2, 23 -- charset="iso-8859-1" 2, 23 -- Subject: TEM/SEM Analysis of Liposomes 2, 23 -- Date: Mon, 30 Mar 2009 19:31:20 -0500 2, 23 -- Message-ID: {27F0D02F0DB26944944521116306B3DA03D61670-at-stl-mail4.stl.umsl.edu} 2, 23 -- X-MS-Has-Attach: 2, 23 -- X-MS-TNEF-Correlator: 2, 23 -- Thread-Topic: TEM/SEM Analysis of Liposomes 2, 23 -- Thread-Index: AcmxmANHVl+YwN2pT1Cq6I/EOKx54w== 2, 23 -- From: "Osborn, David C." {osborndc-at-umsl.edu} 2, 23 -- To: {Microscopy-at-Microscopy.com} 2, 23 -- X-OriginalArrivalTime: 31 Mar 2009 00:31:20.0989 (UTC) FILETIME=[039324D0:01C9B198] 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2V0VNJt019267 ==============================End of - Headers==============================
I have examined liposome suspensions by cryo-TEM in vitreous ice. You should keep in mind that in this, as other techniques, the liposomes are confined to a thin film. Flattening is very likely. This will most likely bias your size distribution measurements from projected area. In such cases we look at the apparent polydispersity and then use a complementary technique, such as dynamic light scattering to study the specimen.
-----Original Message----- X-from: osborndc-at-umsl.edu [mailto:osborndc-at-umsl.edu] Sent: Monday, March 30, 2009 8:32 PM To: jrminter-at-rochester.rr.com
I need help in analyzing 200-300nm liposomes using TEM and SEM. What's the best way to prepare a suspension of liposomes for size analysis? Any suggestions/recommendations would be appreciated.
==============================Original Headers============================== 2, 23 -- From osborndc-at-umsl.edu Mon Mar 30 19:31:24 2009 2, 23 -- Received: from stl-proto10.umsl.edu (STL-PROTO10.umsl.edu [134.124.45.28]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V0VNJt019267 2, 23 -- for {Microscopy-at-Microscopy.com} ; Mon, 30 Mar 2009 19:31:24 -0500 2, 23 -- Received: from stl-mail4.stl.umsl.edu ([134.124.45.88]) by stl-proto10.umsl.edu with Microsoft SMTPSVC(6.0.3790.3959); 2, 23 -- Mon, 30 Mar 2009 19:31:20 -0500 2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 23 -- Content-class: urn:content-classes:message 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Type: text/plain; 2, 23 -- charset="iso-8859-1" 2, 23 -- Subject: TEM/SEM Analysis of Liposomes 2, 23 -- Date: Mon, 30 Mar 2009 19:31:20 -0500 2, 23 -- Message-ID: {27F0D02F0DB26944944521116306B3DA03D61670-at-stl-mail4.stl.umsl.edu} 2, 23 -- X-MS-Has-Attach: 2, 23 -- X-MS-TNEF-Correlator: 2, 23 -- Thread-Topic: TEM/SEM Analysis of Liposomes 2, 23 -- Thread-Index: AcmxmANHVl+YwN2pT1Cq6I/EOKx54w== 2, 23 -- From: "Osborn, David C." {osborndc-at-umsl.edu} 2, 23 -- To: {Microscopy-at-Microscopy.com} 2, 23 -- X-OriginalArrivalTime: 31 Mar 2009 00:31:20.0989 (UTC) FILETIME=[039324D0:01C9B198] 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2V0VNJt019267 ==============================End of - Headers============================== No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.285 / Virus Database: 270.11.33/2031 - Release Date: 03/30/09 17:56:00
==============================Original Headers============================== 9, 22 -- From jrminter-at-rochester.rr.com Mon Mar 30 20:58:30 2009 9, 22 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2V1wUwx007088 9, 22 -- for {Microscopy-at-Microscopy.Com} ; Mon, 30 Mar 2009 20:58:30 -0500 9, 22 -- Received: from parrotxp ([67.240.238.57]) by hrndva-omta04.mail.rr.com 9, 22 -- with ESMTP 9, 22 -- id {20090331015827.RWPM15546.hrndva-omta04.mail.rr.com-at-parrotxp} 9, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 31 Mar 2009 01:58:27 +0000 9, 22 -- From: "John Minter" {jrminter-at-rochester.rr.com} 9, 22 -- To: {Microscopy-at-Microscopy.Com} 9, 22 -- References: {200903310031.n2V0VXQe019397-at-ns.microscopy.com} 9, 22 -- In-Reply-To: {200903310031.n2V0VXQe019397-at-ns.microscopy.com} 9, 22 -- Subject: RE: [Microscopy] TEM/SEM Analysis of Liposomes 9, 22 -- Date: Mon, 30 Mar 2009 21:58:19 -0400 9, 22 -- Message-ID: {000001c9b1a4$2ff46af0$8fdd40d0$-at-rr.com} 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; 9, 22 -- charset="us-ascii" 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Mailer: Microsoft Office Outlook 12.0 9, 22 -- Thread-Index: AcmxmAyFBIQrVVT+Tg+79RJf8fHTCwACxZLA 9, 22 -- Content-Language: en-us ==============================End of - Headers==============================
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Title-Subject: [Filtered] Trinocular adapter for Ernst Leitz Wetzler scopes
Question: Does anyone know whether all Labolux & SM-D Lux scopes will take a trinocular adapter? I am specifically interested in a model from the sixties, serial #674661. Many thanks.
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Title-Subject: [Filtered] Job Opening: Engineer for TEM and SEM service
Question: The Center for Nanostructure Characterization and The School of Materials Science and Engineering, at the Georgia Institute of Technology, seek an electrical Engineer.
Duties: Maintain and repair transmission electron microscopes (JEOL 100CX-II, Hitachi HF-2000, JEOL 4000EX), scanning electron microscopes (LEO 1530, LEO 1550, Hitachi S-800), and specimen preparation equipment (ion mill, PIPS, and ultramicrotome); train and interact with users on the use of the equipment. Other duties as required such as testing of equipment, etc. Hours may vary depending on machine status.
--------------- Qualifications: Bachelor's Degree in Electrical Engineering or related field, or equivalent combination of education and experience. Prefer a Master's Degree.
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The neat thing about the early CH and BH is that they mostly interchangeable between parts--outside of DIC and phase.
Ought not be a big problem. What is the issue?
Dr. Gary Gaugler
At 09:45 PM 3/30/2009, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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The neat thing about the early CH and BH is that they mostly interchangeable between parts--outside of DIC and phase.
Ought not be a big problem. What is the issue?
Dr. Gary Gaugler
At 09:45 PM 3/30/2009, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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We have prepared for SEM liposomes and other nanoparticles by placing them on a filter paper. Please have a look at this publication: Scanning electron microscopy study on nanoemulsions and solid lipid nanoparticles containing high amounts of ceramides.
Hatziantoniou S, Deli G, Nikas Y, Demetzos C, Papaioannou GT.
eikonika-at-otenet.gr yorgosnikas-at-hotmail.com Tel/fax +30 210 8957677 Mobile +30 6945 107477
----- Original Message ----- X-from: {osborndc-at-umsl.edu} To: {eikonika-at-otenet.gr} Sent: Tuesday, March 31, 2009 3:37 AM
Nell’ambito del VII° Forum della FIST - Geoitalia2009 (Rimini 9-11 settembre 2009; http://www.geoitalia.org/), stiamo organizzando la sessione tematica D7 “Il particolato minerale: origine, aspetti cristallochimici, risvolti ambientali e sanitari” e il corso breve SC 3 “Particolato minerale: origine, campionamento, analisi, potenzialità di inquinamento e di rischio, risanamento”.
La sessione D7 si prefigge di fare il punto sulle conoscenze relative al particolato minerale nelle varie frazioni dimensionali e nei vari ambienti di origine e/o di recupero, tenendo in considerazione che polveri sottili, particolato e fibre minerali presentano ormai da anni grande interesse ed attenzione e che, comunque, determinati aspetti del problema devono essere ancora affrontati ed interpretati. Saranno pertanto considerate e discusse le attuali metodologie di campionamento, la caratterizzazione mineralogica sia delle fasi primarie sia di quelle secondarie, le interazioni con l’ambiente e con gli esseri umani, le cause di aero-dispersione, le possibili soluzioni per la prevenzione e il risanamento.
Il corso breve SC 3 verterà sui temi della sessione D7, puntando in particolare sugli aspetti analitici, tossicologici e normativi della problematica. Il corso è rivolto a ricercatori, studiosi ed operatori del settore.
Con l’obiettivo di scambiare informazioni sullo stato delle conoscenze e di incentivare collaborazioni interdisciplinari, Vi invitiamo ad inviare contributi orali e/o poster per la sessione D7 inerenti i vari aspetti del “particolato minerale”.
Il termine ultimo per l’invio dei riassunti è il 16 maggio 2009.
Scusandoci per invii multipli, inviamo cordiali saluti, Elena Belluso (elena.belluso-at-unito.it) Antonio GIANFAGNA (antonio.gianfagna-at-uniroma1.it) Alessandro GUALTIERI (alessandro.gualtieri-at-unimore.it)
--------------------------------------------------------------------------------------------------------------------- (we apologize for multiple postings)
In the context of the 7th Forum Geoitalia 2009 (9-10-11 September 2009, http://www.geoitalia.org) we are organizing the D7 thematic session titled “Mineral particulate: origin, crystal chemical aspects, and related environmental and health issues”, and the SC 3 short course “Mineral particulate: origin, sampling, analyses, pollution and risk potentiality, reclamation”.
The D7 thematic session aims to fix the actual knowledge related to the particulate mineral, the various dimensional fractions, and the different environments of origin and recovering, taking into account that thin dusts, particulate, and mineral fibers have given rise to particular attention and interest for many years, but specific aspects of their interactions with different environments still need to be explored and understood.
Sampling methodologies and mineralogical characterization of the primary and secondary mineral phases will be considered. Moreover, the interactions between the environment and humans, the causes of the air-dispersion, and the possible solutions for prevention and reclamation will also be subject of debate.
The SC 3 short course is strongly correlated to the D7 thematic session and aims at developing specific arguments on the analysis, toxicology and Italian regulations on the mineral particulate issue. The course is addressed to researchers, scientists, and people operating in this topic.
In order to exchange information regarding the actual knowledge and to increase interdisciplinary collaborations, we invite you to send contributions (talks and posters) about the different topics of the “mineral particulate” for the D7 thematic session
Note the abstract deadline is May 16, 2009.
Best regards,
the conveners Elena Belluso (elena.belluso-at-unito.it) Antonio GIANFAGNA (antonio.gianfagna-at-uniroma1.it) Alessandro GUALTIERI (alessandro.gualtieri-at-unimore.it)
----------------------------------------------------------------------------------- Prof. Elena BELLUSO - Ph.D. Mineralogy and Crystallography Dipartimento di Scienze Mineralogiche e Petrologiche Universita' degli Studi di Torino Via Valperga Caluso, 35 I-10125 TORINO - ITALIA tel: (39) 011 670 51 35 - fax: (39) 011 670 51 28 e-mail: elena.belluso-at-unito.it http://www.dsmp.unito.it ----------------------------------------------------------------------------------- "I've... seen things you people wouldn't believe. Attack ships on fire off the shoulder of Orion. I watched C-beams... glitter in the dark near the Tanhauser Gate. All those... moments will be lost... in time..., like... tears... in... rain." Blade Runner
I'm having problems with detached retina, not mine personally but samples I receive.
When I embed 3mm punch biopsies I have to bisect them. This causes the retina to detach from the back of the punch.
I've tried simple agar sandwiches--agar in a plate, retina on top and then a drop of agar on top of the punch--they separate when bisected.
They separate if you look at them wrong.
If you can give suggestions as to how to keep my retina attached it would be great.
I'd love to "see" how you keep them together.
Thanks,
Paula :-)
p.s. if you were alive in 1973 and want a radio music flashback google "Life on Mars Radio". It's playing great old rock & roll, some real deep cuts.
I have no relationship to this radio station, other than enjoying it before it goes off the internet when the series ends.
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 17, 25 -- From vapatpxs-at-yahoo.com Tue Mar 31 12:15:44 2009 17, 25 -- Received: from n27.bullet.mail.mud.yahoo.com (n27.bullet.mail.mud.yahoo.com [68.142.206.222]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n2VHFiU8025537 17, 25 -- for {microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 12:15:44 -0500 17, 25 -- Received: from [209.191.108.97] by n27.bullet.mail.mud.yahoo.com with NNFMP; 31 Mar 2009 17:15:43 -0000 17, 25 -- Received: from [68.142.201.243] by t4.bullet.mud.yahoo.com with NNFMP; 31 Mar 2009 17:15:43 -0000 17, 25 -- Received: from [127.0.0.1] by omp404.mail.mud.yahoo.com with NNFMP; 31 Mar 2009 17:15:43 -0000 17, 25 -- X-Yahoo-Newman-Property: ymail-3 17, 25 -- X-Yahoo-Newman-Id: 920524.6369.bm-at-omp404.mail.mud.yahoo.com 17, 25 -- Received: (qmail 14003 invoked by uid 60001); 31 Mar 2009 17:15:43 -0000 17, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238519743; bh=ZYnf3nzJUA5WLYz5ercnGo2fjhImBMHxZiy9uO2Na1I=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=4SLZoGkE7Pr4ENkBiEjl9GUtRaBUy9H9Zp/maLjg/vxOPG7s2aNYQfbxKltboeoeTrsyJMmDkHmqx73eVCzpbJoBCvcVhpKmcjCESSojhx8hfb8CBy/15kVLRu7z/U+Kctui5/uAADDfA3oa3F1Jkql7pgV7yglZ8fqFoKEhmis= 17, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 25 -- s=s1024; d=yahoo.com; 17, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; 17, 25 -- b=wItH0Kco7yQeTtjkf15vvuM+oMdTQjsD2l4MxkiZoZMfTGxWsMMlK6AEFJLTBiAbsH4fBUnrbr0ukk2GdoTX4SfKr1ZQTS3M2bxlQ3HbvDELsyCdvXdFuWLZZabBthmCS0yZh0OT0WjqmLU2LmjqFwPI8tRtYVrGiydLAlTvP+g=; 17, 25 -- Message-ID: {85710.1316.qm-at-web46110.mail.sp1.yahoo.com} 17, 25 -- X-YMail-OSG: 7lnM4OMVM1n_FVEYME2awnnc2WQia8Kex6dF84Ik.Ldx2XNKvFcRh6P.i3Tx.7mVTqNQ7loyPb3YHTzEcscJ1FBiIrPEgGZkqR8dRJMu8cPJAaCsdZ_UlHUCsZQT3o6vA81NRFHBYXo.92YYsEzKDoFKMNN53wxbpy2d1R14Tu9PH7MWqU1SKIDXpcMT1t4ese0Ck4opRUI4g2Y1cG.GkVydondIRzbkiNDg2KeW19mzVof765HO9ZuqAf8UyoIjag_8LY5MHTZ53d65OiQ- 17, 25 -- Received: from [132.239.85.200] by web46110.mail.sp1.yahoo.com via HTTP; Tue, 31 Mar 2009 10:15:43 PDT 17, 25 -- X-Mailer: YahooMailClassic/5.1.20 YahooMailWebService/0.7.289.1 17, 25 -- Date: Tue, 31 Mar 2009 10:15:43 -0700 (PDT) 17, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 17, 25 -- Subject: Detached retina 17, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 17, 25 -- MIME-Version: 1.0 17, 25 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
Hi Paula- I have to admit that I liked the BBC version of LIfe On Mars better (even if scenes for the first episode for the ABC series were filmed in the park near my house in Queens - not Central Park as portrayed).
For your retinas...are you sure they are not already detached by the biopsy procedure? I've dealt with many rat eyes, so I know what you are going through. They always split at the RPE/ORS interface. If you are fairly confident that they are intact when you get them, leave them whole through the processing....just drag it out terribly (30 minute dehydrations, many-stepped infiltration w/o accelerater, drawn out over 2-3 days before going into final resin) and then, when they are fully infiltrated, bisect them by cutting from the neural retina down through the sclera. You may still get detachments along the edge, but the middle should stay put.
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-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Dear listmembers.... We are looking for a SEM and TEM to receive donations on behalf of our partners, the Del Plata Adventist University . Both instruments must be in working condition. All costs of packing and shipping will be paid by us
Please, contact us via email to info-at-fundatel.org.ar
Best Regards
-- Fundatel Fundación de Telemedicina Victoria 144 Parana Entre Rios Argentina
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I would probably look into one of John Russ's books on image processing and measurement. I don't have an exact citation. Working in the field, I would say this issue is practically intuitively obvious when explained.
You should understand that measurements will always be plus or minus one (or more) pixels. I would be comfortable with 2-micron pixels to detect 6-micron features, but the exact diameter or area will be less precise. I like to have more than 10 pixels across the diameter of a moderately sized particle. I could live with fewer pixels across the diameter on the smaller end of the distribution.
However, there is also often a significant effect due to the threshold setting. Fuzziness on the edge leads to larger particles if the threshold is set closer to the background brightness. A setting midway between background and feature brightness is preferred to evenly split the uncertainty.
One of the corollaries to that principle is that small particles will suffer underestimation as their maximum brightness will often be less than that of larger particles. For instance, if the fuzziness of the edge extends over 3 pixels on each side, and the particle is only 5 pixels wide, then the center pixel is going to be less than full brightness. The particle will appear smaller than its true size. More magnification (not just more pixels) may be necessary to achieve full brightness and eliminate the effect. This is fairly easy to demonstrate on an SEM with a brightness waveform display. I suppose you should also be able to visualize this effect by plotting brightness along a linear traverse across various sized particles.
IMHO, it is better to understand many of these principles for yourself and be able to explain them and your choices than to blithely state that a procedure was done in accordance with some numbered procedure. Standard procedures do not guarantee accuracy (as I can attest from some ASTM coal analysis procedures). However, they do guarantee that everyone will be wrong in the same way and presumably to the same extent.
Warren S.
X-from: Jacqueline.Ayotte-at-ticona.com [mailto:Jacqueline.Ayotte-at-ticona.com] Sent: Mon 3/30/2009 8:39 AM To: wesaia-at-iastate.edu
Hello All,
Is there a documented rule of thumb regarding minimum resolution per pixel when measuring particles of a certain size range in an image? For example, if one were interested in measuring particles down to 6 microns would one set magnification to yield 3 microns per pixel or 2 microns per pixel in the acquired image? (or less?)
Again, looking for documentation on this. I need to demonstrate proper resolution to peers in Europe and it is not enough that I just "say so" without any supportive data.
Thank you for any help or pointers.
Regards, Jackie
Jacqueline Ayotte Microscopist - Advanced Materials Characterization Ticona 8040 Dixie Highway Florence KY 41042 859-372-3139 fax 859-372-3184 jacqueline.ayotte-at-ticona.com The information contained in this e-mail, and any attachments thereto, is confidential and is intended only for use by the individual(s) and/or entity named above. If you are not the intended recipient of this e-mail, you are hereby notified that any dissemination, distribution or copying of this communication or any disclosure of the contents of this communication to others is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this e-mail. Please then delete the original including all attachments and any copy of any e-mail and printout thereof.
==============================Original Headers============================== 11, 27 -- From Jacqueline.Ayotte-at-ticona.com Mon Mar 30 08:35:44 2009 11, 27 -- Received: from exprod7og114.obsmtp.com (exprod7og114.obsmtp.com [64.18.2.215]) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 35 -- From wesaia-at-iastate.edu Tue Mar 31 16:13:28 2009 16, 35 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VLDRLf021994 16, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 16:13:27 -0500 16, 35 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 16, 35 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n2VLDQRW016922 16, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 16:13:26 -0500 16, 35 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 16, 35 -- id 7304_c6fce34a_1e38_11de_8969_001372578af6; 16, 35 -- Tue, 31 Mar 2009 16:13:27 -0500 16, 35 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 16, 35 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n2VLD4uv003782 16, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 16:13:19 -0500 16, 35 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 35 -- Tue, 31 Mar 2009 16:12:58 -0500 16, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 35 -- Content-class: urn:content-classes:message 16, 35 -- MIME-Version: 1.0 16, 35 -- Content-Type: text/plain; 16, 35 -- charset="iso-8859-1" 16, 35 -- Subject: RE: [Microscopy] OM - resolution rule of thumb? 16, 35 -- Date: Tue, 31 Mar 2009 16:10:15 -0500 16, 35 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703C246AE-at-maire.eng.iastate.edu} 16, 35 -- X-MS-Has-Attach: 16, 35 -- X-MS-TNEF-Correlator: 16, 35 -- Thread-Topic: [Microscopy] OM - resolution rule of thumb? 16, 35 -- thread-index: AcmxPPPodyQy53QlRXSh93lkgKzm1gAAGnjy 16, 35 -- References: {200903301339.n2UDdPuk025070-at-ns.microscopy.com} 16, 35 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 16, 35 -- To: {Jacqueline.Ayotte-at-ticona.com} , {Microscopy-at-microscopy.com} 16, 35 -- X-OriginalArrivalTime: 31 Mar 2009 21:12:58.0371 (UTC) FILETIME=[77798530:01C9B245] 16, 35 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.3.31.205818 16, 35 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 16, 35 -- Content-Transfer-Encoding: 8bit 16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n2VLDRLf021994 ==============================End of - Headers==============================
Just saying thankyou to everyone who replied to my query re staining for glycogen in resin-embedded tissue (see below). Personally I agree with Geoff McAuliffe and others who suggested morphology should suffice. He commented that not all hepatocytes contain the same amount of glycogen which I found interesting and reminded me of the uncertainty related to sampling issues in TEM studies. Our researcher has demonstrated a rise in intracellular glycogen in several of his tests so I believe he is genuinely onto something and is not being duped by the vagaries of TEM sampling issues. Thanks to Terry Robertson, Stephane and others who provided protocols for various staining techniques. Thanks to Wolfgang Muss and Donald Gantz for their comprehensive replies and their comments regarding pH issues and the leaching of glycogen when using en-bloc staining with uranyl acetate.
Regards,
John Brealey
Hi,
We have a researcher who is studying the effects of a particular drug on rat liver, heart and spinal cord. Tissue for EM was processed with osmium tetroxide, en-bloc stained with uranyl acetate, dehydrated with alcohols and embedded in Procure 812 epoxy resin. Thin sections were stained with lead citrate. By EM we found increased amounts of glycogen in one of the liver samples. The researcher asked "How do you know it's glycogen". I said "It just is". He is worried that his supervisor won't accept that it's glycogen just because we said so. He wants to prove it.
So my question is...
Is there a stain for epoxy resin sections that will stain specifically for glycogen? Will periodic acid Schiff (PAS) work on resin-embedded sections?
Regards,
John Brealey
Supervisor - Electron Microscope Unit
E john.brealey-at-imvs.sa.gov.au
T 8222 6612
F 8222 6425
www.sapathology.sa.gov.au
SA Pathology (Queen Elizabeth Hospital)
Electron Microscope Unit, Surgical Pathology
SA Pathology
Queen Elizabeth Hospital
Woodville, 5011
AUSTRALIA
==============================Original Headers============================== 27, 27 -- From john.brealey-at-imvs.sa.gov.au Tue Mar 31 17:57:49 2009 27, 27 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 27, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n2VMvmvM007557 27, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Mar 2009 17:57:49 -0500 27, 27 -- X-IronPort-AV: E=Sophos;i="4.39,304,1235914200"; 27, 27 -- d="scan'208";a="16432857" 27, 27 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 27, 27 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 01 Apr 2009 09:27:46 +1030 27, 27 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 27, 27 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 27, 27 -- 8.1.263.0; Wed, 1 Apr 2009 09:26:43 +1030 27, 27 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 27, 27 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id BE8BA34047 for 27, 27 -- {Microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 09:27:46 +1030 (CST) 27, 27 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 27, 27 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 27, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 27, 27 -- Subject: Comments on Glycogen - Thankyou! 27, 27 -- Date: Wed, 1 Apr 2009 09:27:46 +1030 27, 27 -- Organization: IMVS 27, 27 -- Message-ID: {000901c9b254$1b743f50$7d8a140a-at-41347i} 27, 27 -- MIME-Version: 1.0 27, 27 -- Content-Type: text/plain; charset="us-ascii" 27, 27 -- Content-Transfer-Encoding: 7bit 27, 27 -- X-Mailer: Microsoft Office Outlook 11 27, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 27, 27 -- Thread-Index: AcmyVBtOs6JuFEyqTsKzJfhQAIwhJg== ==============================End of - Headers==============================
Our gold target has ended its useful life and there I am with 4,5 grams of pure gold which does not belong to me. I would feel bad to take it at home, and even worse to dump it with the heavy metals ;-) Now I cannot say that targets are very complex pieces of technology, so I suppose that the expensive price of gold targets is mainly explained by the price of the metal itself, meaning that recycling would be a very precious way to acquire a new target for fewer cents (or are they dollars?). Our current provider told us they don't recycle. Is there someone here who does? (in Europe)
I worked for a precious metal refiner for a short while in Los Angeles. Most refiners will certainly process and refine your gold scrap. You can easily find a local one in EC by just Google. It may not be worth the minimum processing fee for 4g of gold. Do you have other gold or Pt scrap to consolidate?
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Wednesday, April 01, 2009 1:06 AM To: dkloos-at-parallaxray.com
Dear all,
Our gold target has ended its useful life and there I am with 4,5 grams of pure gold which does not belong to me. I would feel bad to take it at home, and even worse to dump it with the heavy metals ;-) Now I cannot say that targets are very complex pieces of technology, so I suppose that the expensive price of gold targets is mainly explained by the price of the metal itself, meaning that recycling would be a very precious way to acquire a new target for fewer cents (or are they dollars?). Our current provider told us they don't recycle. Is there someone here who does? (in Europe)
In Europe (i.e. at least Austria&Germany) two } recyclers { for precious (heavy) metals I know of:
E.g. A) see http://www.degussa.com/degussa/en/ (English) ==} as of 12th September 2007 DEGUSSA is part of ==} EVONIK ==} www.evonik.com http://www.degussa.com/degussa/de/ (German)
==} find "SEARCH" ==} "metals" ==} result = Base Metal Refining Services: Contact: Evonik Degussa GmbH Business Line Catalysts Rodenbacher Chaussee 4 63457 Hanau Germany T: +49-6181-59-8722 F: +49-6181-59-2699 degussa_catalysts-at-degussa.com
E.g. B) AUSTRIA: OEGUSSA http://www.oegussa.at/ ==} click in menuebar Recycling: ==} http://www.oegussa.at/neu/recycling/index.htm
Gold-, silber-, platin- oder palladiumhältiges Scheidgut Ansprechpartner: Erich Siegler 01-866 46 DW 4153 01-866 46 DW 4154 Oder in Ihrer Ögussa-Filiale: http://www.oegussa.at/neu/standorte/filialen.htm
==} Wien, Gumpendorfer Straße 85, A 1060 Wien Tel. +43 1 599 61 - 225 Fax +43 1 599 61 - 310
You won't get easy rid of your gold target perhaps, despite the global gold price at the moment is quite good... Important to know (IMHO) is the quality / pure Au - metal content of the target left.
Another possibility would be : offer to jeweler, watch maker(s) on a "private honorary" basis...
Best wishes and good luck, Mit besten Gruessen,
Wolfgang Muss Salzburg, Austria
} -----Ursprüngliche Nachricht----- } Von: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Gesendet: Mittwoch, 01. April 2009 10:01 } An: Muß Wolfgang } Betreff: [Microscopy] gold target: second life? } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } Dear all, } } Our gold target has ended its useful life and there I am with } 4,5 grams of pure gold which does not belong to me. } I would feel bad to take it at home, and even worse to dump } it with the heavy metals ;-) } } Now I cannot say that targets are very complex pieces of } technology, so I suppose that the expensive price of gold } targets is mainly explained by the price of the metal itself, } meaning that recycling would be a very precious way to } acquire a new target for fewer cents (or are they dollars?). } Our current provider told us they don't recycle. } Is there someone here who does? (in Europe) } } Regards, } } Stephane } } } } } } ==============================Original } Headers============================== } 8, 20 -- From nizets2-at-yahoo.com Wed Apr 1 02:55:51 2009 } 8, 20 -- Received: from web110814.mail.gq1.yahoo.com } (web110814.mail.gq1.yahoo.com [67.195.13.237]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id n317tpEg015074 } 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr } 2009 02:55:51 -0500 } 8, 20 -- Received: (qmail 24639 invoked by uid 60001); 1 Apr } 2009 07:55:50 -0000 } 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; } c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238572550; } bh=EuQbKyN2f2/legr/F7ekHo2WkZTg2p/6tLfBsuwnQmg=; } h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T } o:MIME-Version:Content-Type; } b=QE1N0SMjJxCWwdijFmnPCpZQv8gYQykezebiNtLpR7nUSux7WBhq6Yxqt8Q6 } 8J41JkbaSK16Nptj7nOBTOPGQtIUsWby2gVW8Sg9A3w8EyiDHeO5puU7ggypMA } 42R8P0uWCyyZ3pZhK7GYh0mIbSRdSXafRdMYv9BT5ANCkHHt8= } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- } h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:T } o:MIME-Version:Content-Type; } 8, 20 -- } b=v+tmtRG/yh4b1j/m5wpKI2HPD05Zx+ehlHJP0gD7TX1mWdVJcZw2QLC73z2W } qDiil4PyXzkekD75fATFTOpaTDD28kkREZAhHSJ9537XrYshaIkwH2ozjeqik2 } vBR5Ldtb8EMybqN0mohvTOigNk+dBpkfh7OvirT61cpS4a2cc=; } 8, 20 -- Message-ID: {567818.1924.qm-at-web110814.mail.gq1.yahoo.com} } 8, 20 -- X-YMail-OSG: } 9zmZ0VkVM1liV6mucRVbtfa7C27Ihkr2A87_D1Op4Z9zPtfxPo5aP8t0fhxCLJ } IOUyJowZuXMdA0EHvYvO9b3RMvNPTIzqY0HJoMt.Dd17A5Wk07UrhQiQsNBOn3 } _psq1BWxEx4Hqooq50pn9AruHygSmey5r8esiqQXDLJKJzPWObFyx7eundx5MR } TC_fwOXcxcLiDkkkXXjDjmCJjsod.BmXknh2WtUGSTcwEwbHCahplAS9engGNZ } vVS4R0KC61xhLGetcAtCCUf1E8OhpFbvEELGBQ34YT7aBCVm9NH.6j23xtoM7g-- } 8, 20 -- Received: from [80.122.101.100] by } web110814.mail.gq1.yahoo.com via HTTP; Wed, 01 Apr 2009 00:55:50 PDT } 8, 20 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1 } 8, 20 -- Date: Wed, 1 Apr 2009 00:55:50 -0700 (PDT) } 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 20 -- Subject: gold target: second life? } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - } Headers============================== }
==============================Original Headers============================== 19, 36 -- From W.Muss-at-salk.at Wed Apr 1 03:27:21 2009 19, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 19, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n318RLaa006637 19, 36 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 03:27:21 -0500 19, 36 -- Received: from localhost (localhost [127.0.0.1]) 19, 36 -- by hermes.salk.at (Postfix) with ESMTP id 3398CC385D; 19, 36 -- Wed, 1 Apr 2009 10:27:20 +0200 (CEST) 19, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 19, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 19, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 19, 36 -- with ESMTP id e3jYBbHFsVJ9; Wed, 1 Apr 2009 10:27:19 +0200 (CEST) 19, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 19, 36 -- by hermes.salk.at (Postfix) with ESMTP id BF2F4C3832; 19, 36 -- Wed, 1 Apr 2009 10:27:19 +0200 (CEST) 19, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 19, 36 -- Wed, 1 Apr 2009 10:27:20 +0200 19, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 36 -- Content-class: urn:content-classes:message 19, 36 -- MIME-Version: 1.0 19, 36 -- Content-Type: text/plain; 19, 36 -- charset="iso-8859-1" 19, 36 -- Subject: [Microscopy] Re: gold target: second life? 19, 36 -- Date: Wed, 1 Apr 2009 10:27:19 +0200 19, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0938-at-N1RZ116.lksdom21.lks.local} 19, 36 -- In-Reply-To: {200904010800.n3180ZGK021610-at-ns.microscopy.com} 19, 36 -- X-MS-Has-Attach: 19, 36 -- X-MS-TNEF-Correlator: 19, 36 -- Thread-Topic: [Microscopy] Re: gold target: second life? 19, 36 -- Thread-Index: Acmyn/V7F/69LWGpRUWkFW98eQpQpQAAY4Lw 19, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 19, 36 -- To: {nizets2-at-yahoo.com} 19, 36 -- Cc: {microscopy-at-microscopy.com} 19, 36 -- X-OriginalArrivalTime: 01 Apr 2009 08:27:20.0021 (UTC) FILETIME=[AC7F5850:01C9B2A3] 19, 36 -- X-Scanned-By: SALK-Content-Filter 19, 36 -- Content-Transfer-Encoding: 8bit 19, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n318RLaa006637 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michelle.gignac-at-duke.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Thanks to all that responded to the first message.
The microtome is an AO/Reichert Ultracut, Type 701701. I could not find a model designation on it. It looks like an all grey Ultracut E. The control unit is different. It does not have the cutting thickness controls on it. I was fortunate to receive a copy of this manual from another listserve member (Thanks again Dale!). If anyone has a pdf version, I would appreciate a copy.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wzhe-at-laurentian.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wzhe-at-laurentian.ca Name: William
Organization: Luarentian Univ
Title-Subject: [Filtered] Imaging plates for TEM Photo
Question: Dear there,
We want to use Fuji imaging plates in TEM photo work and read some recommend on product FDL-5000 plate. If someone could comment on this imaging plate
1. What performance looks like in TEM photos? 2. Any alternate we can try, because this plate is quite expensive to start with, ~US$4000 for a 16 pieces/pack ?
I was in the process of changing the HBO 100 in my Zeiss Photomik III when I discovered a broken condenser lens. After waiting for the lens to arrive (made by bridge trolls in Austria) and having my lab shut down for 6 weeks for A/C repairs, I've forgotten how to put the lamp housing and bulb assembly back together.
My original Zeiss instructions show a grounding pin and I have a grounding wire. I think it has to connect to the diffuser thingy that the top of the bulb goes into but I can't figure out where. It could attach to the bottom but doesn't look like it by the way the grounding wire is bent.
If anyone can help me it would be great.
That's what I get for not taking notes. It made sense at the time but my old lady brain forgot the original configuration after almost 3 months after taking it apart.
Please don't nag me for not putting it together again once I discovered the broken lens condenser. I know that now.
It is a beautiful microscope with great optics and it looks like Giger designed it. I could look at it all day but I really want to look through it for lovely fluorescence and light images.
Thanks in advance for any help/suggestions you can send my way.
Paula :-}
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 15, 25 -- From vapatpxs-at-yahoo.com Wed Apr 1 17:47:58 2009 15, 25 -- Received: from n59.bullet.mail.sp1.yahoo.com (n59.bullet.mail.sp1.yahoo.com [98.136.44.43]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n31MltBj005912 15, 25 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 17:47:57 -0500 15, 25 -- Received: from [69.147.84.144] by n59.bullet.mail.sp1.yahoo.com with NNFMP; 01 Apr 2009 22:47:54 -0000 15, 25 -- Received: from [69.147.65.152] by t6.bullet.mail.sp1.yahoo.com with NNFMP; 01 Apr 2009 22:47:54 -0000 15, 25 -- Received: from [127.0.0.1] by omp400.mail.sp1.yahoo.com with NNFMP; 01 Apr 2009 22:47:54 -0000 15, 25 -- X-Yahoo-Newman-Property: ymail-3 15, 25 -- X-Yahoo-Newman-Id: 381515.93336.bm-at-omp400.mail.sp1.yahoo.com 15, 25 -- Received: (qmail 71772 invoked by uid 60001); 1 Apr 2009 22:47:54 -0000 15, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238626074; bh=yi/ySkCnQdEBuV//1r5VM+Q8HcQtYGmz2Kz9f4A76hg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=3iqgALkf31j/1pZ/jOxdFZ5daqV+JrcCfNa7Ryo2AVH0LmB7DrHwnrWWA7Vfnpe7u21FpVAAfC55C1SShdJnBnhiIHVdgWhu3RPjpnFz7PuLZsvqxXE7trfkzEr9zzWRY4iVJs4t85+NwJc1Cw+0p2S65nfEG9LOoDS0v9iSTec= 15, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 25 -- s=s1024; d=yahoo.com; 15, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; 15, 25 -- b=cqymkDPdotRlVwJLCarm1vrnlrli9SkmwpLXEZUWUNNVNIbbqmKY+0jxb/TMcZoeCI3MnFIBWfYc3gHfAYYA8KoSSRexKgjcgjGiseN9xtyXq/Loj+I5RscFFXhwJ5hSemNMf3ykG2+fgQWJY1r1YnpPhcFZQH/M1s8zqveRI5E=; 15, 25 -- Message-ID: {250358.71222.qm-at-web46108.mail.sp1.yahoo.com} 15, 25 -- X-YMail-OSG: tf2U8tMVM1n2QFDEC00xqk4K7hlVauLxV0bnbxuplB_tNFAUHp6LEgCoiJa_Xy3jLQHs19V8hQz49PBsswW8KnFJH94RZsqxOmI.ZVHwFdCz7epwH4JTxmMYYa3xux4f9ZsfQ7MfeTTZfj2l8CYIC8R2Lv.9ufyBT_wOaBqD5zCNTLDj1UGcF_p8E5GY.whPAfRo.DcCq1_8UJYvZeWzkFFN._UERgCxA5UP7Z2OyPgjw73jme.6Hx3qE_CbKI45h7P8g8VjjEAIAN.Kw48- 15, 25 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com via HTTP; Wed, 01 Apr 2009 15:47:54 PDT 15, 25 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1 15, 25 -- Date: Wed, 1 Apr 2009 15:47:54 -0700 (PDT) 15, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 15, 25 -- Subject: Zeiss Photomik III 15, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 15, 25 -- MIME-Version: 1.0 15, 25 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
I'm looking for an instruction manual for a Fisher Histomatic 166A tissue processor. A PDF or a xerox copy would be great. Off course I'm willing to pay the costs!
These manuals seem very hard to find and I begin to think that Fisher didn't even bother to make one...
I asked the Fisher representatives in Belgium. Their answer: "O, did we manufacture those?". Well... At least they answered.
I give my old sputter targets to a jeweler which remelts it and laminate a new foil (0.2-0.3 mm thick). I cut then 2-3 disks (~50 mm diameter) in the foil, and the falls return to the box until the next remelting. It cost me a few tens of euro all 2-3 years.
Of coarse it's only jewelry 24 carats purity and not 12N ! But it's enough for the few works which need gold coating. And it's the same gold we use for UHV gaskets, which the jeweler makes whith the old flatten ones.
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
nizets2-at-yahoo.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear all, } } Our gold target has ended its useful life and there I am with 4,5 grams of pure gold which does not belong to me. } I would feel bad to take it at home, and even worse to dump it with the heavy metals ;-) } Now I cannot say that targets are very complex pieces of technology, so I suppose that the expensive price of gold targets is mainly explained by the price of the metal itself, meaning that recycling would be a very precious way to acquire a new target for fewer cents (or are they dollars?). Our current provider told us they don't recycle. } Is there someone here who does? (in Europe) } } Regards, } } Stephane } } } } } } ==============================Original Headers============================== } 8, 20 -- From nizets2-at-yahoo.com Wed Apr 1 02:55:51 2009 } 8, 20 -- Received: from web110814.mail.gq1.yahoo.com (web110814.mail.gq1.yahoo.com [67.195.13.237]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n317tpEg015074 } 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 1 Apr 2009 02:55:51 -0500 } 8, 20 -- Received: (qmail 24639 invoked by uid 60001); 1 Apr 2009 07:55:50 -0000 } 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238572550; bh=EuQbKyN2f2/legr/F7ekHo2WkZTg2p/6tLfBsuwnQmg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QE1N0SMjJxCWwdijFmnPCpZQv8gYQykezebiNtLpR7nUSux7WBhq6Yxqt8Q68J41JkbaSK16Nptj7nOBTOPGQtIUsWby2gVW8Sg9A3w8EyiDHeO5puU7ggypMA42R8P0uWCyyZ3pZhK7GYh0mIbSRdSXafRdMYv9BT5ANCkHHt8= } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; } 8, 20 -- b=v+tmtRG/yh4b1j/m5wpKI2HPD05Zx+ehlHJP0gD7TX1mWdVJcZw2QLC73z2WqDiil4PyXzkekD75fATFTOpaTDD28kkREZAhHSJ9537XrYshaIkwH2ozjeqik2vBR5Ldtb8EMybqN0mohvTOigNk+dBpkfh7OvirT61cpS4a2cc=; } 8, 20 -- Message-ID: {567818.1924.qm-at-web110814.mail.gq1.yahoo.com} } 8, 20 -- X-YMail-OSG: 9zmZ0VkVM1liV6mucRVbtfa7C27Ihkr2A87_D1Op4Z9zPtfxPo5aP8t0fhxCLJIOUyJowZuXMdA0EHvYvO9b3RMvNPTIzqY0HJoMt.Dd17A5Wk07UrhQiQsNBOn3_psq1BWxEx4Hqooq50pn9AruHygSmey5r8esiqQXDLJKJzPWObFyx7eundx5MRTC_fwOXcxcLiDkkkXXjDjmCJjsod.BmXknh2WtUGSTcwEwbHCahplAS9engGNZvVS4R0KC61xhLGetcAtCCUf1E8OhpFbvEELGBQ34YT7aBCVm9NH.6j23xtoM7g-- } 8, 20 -- Received: from [80.122.101.100] by web110814.mail.gq1.yahoo.com via HTTP; Wed, 01 Apr 2009 00:55:50 PDT } 8, 20 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1 } 8, 20 -- Date: Wed, 1 Apr 2009 00:55:50 -0700 (PDT) } 8, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 20 -- Subject: gold target: second life? } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Apr 2 05:22:20 2009 8, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32AMJln027062 8, 32 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 05:22:20 -0500 8, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n32AMIRn066497 8, 32 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 12:22:18 +0200 (CEST) 8, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 32 -- (No client certificate requested) 8, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 37C9A3EC003 8, 32 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 12:20:33 +0200 (CEST) 8, 32 -- Message-ID: {49D491CE.6080202-at-ipcms.u-strasbg.fr} 8, 32 -- Date: Thu, 02 Apr 2009 12:22:06 +0200 8, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 32 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318) 8, 32 -- MIME-Version: 1.0 8, 32 -- To: Microscopy-at-microscopy.com 8, 32 -- Subject: Re: [Microscopy] gold target: second life? 8, 32 -- References: {200904010802.n3182H26024078-at-ns.microscopy.com} 8, 32 -- In-Reply-To: {200904010802.n3182H26024078-at-ns.microscopy.com} 8, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 32 -- Content-Transfer-Encoding: 8bit 8, 32 -- X-IPCMS-MailScanner: Found to be clean 8, 32 -- X-IPCMS-MailScanner-SpamScore: sss 8, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.154]); Thu, 02 Apr 2009 12:22:18 +0200 (CEST) 8, 32 -- X-Virus-Scanned: ClamAV 0.94.2/9198/Thu Apr 2 08:50:41 2009 on mr4.u-strasbg.fr 8, 32 -- X-Virus-Status: Clean 8, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST 8, 32 -- autolearn=disabled version=3.2.5 8, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr ==============================End of - Headers==============================
Thank you to all for your answers/comments! We have only 1 target, it is too few for anybody to care about this amount alone. But I was directed to a company in Germany called "BALTIC Präparation" which recycles the gold and deduces a part of its value from the new target: I give this information in case anybody else need it too.
For those in north America, I believe that Abe Dayani of Recycling Systems Inc. will accept old targets and give credit toward the purchase of new ones. They are also a great source targets made to order at lower cost than OEM targets.
No connection with them except satisfied customer for many years.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, April 02, 2009 10:04 AM To: Tindall, Randy D.
Thank you to all for your answers/comments! We have only 1 target, it is too few for anybody to care about this amount alone. But I was directed to a company in Germany called "BALTIC Präparation" which recycles the gold and deduces a part of its value from the new target: I give this information in case anybody else need it too.
That should have been REFINING Systems Inc., not Recycling. My bad.
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 5, 27 -- From TindallR-at-missouri.edu Thu Apr 2 10:15:49 2009 5, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32FFni5014879 5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 2 Apr 2009 10:15:49 -0500 5, 27 -- X-IronPort-Anti-Spam-Filtered: true 5, 27 -- X-IronPort-Anti-Spam-Result: ApoEABhz1EnRauUo/2dsb2JhbADAdAEJhx2ITYJKgTIG 5, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 5, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 02 Apr 2009 10:15:49 -0500 5, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 27 -- Thu, 2 Apr 2009 10:15:43 -0500 5, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 27 -- Content-class: urn:content-classes:message 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; 5, 27 -- charset="us-ascii" 5, 27 -- Subject: Correction 5, 27 -- Date: Thu, 2 Apr 2009 10:15:15 -0500 5, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FA6-at-UM-XMAIL08.um.umsystem.edu} 5, 27 -- X-MS-Has-Attach: 5, 27 -- X-MS-TNEF-Correlator: 5, 27 -- Thread-Topic: Correction 5, 27 -- Thread-Index: AcmzpdNk49omzGUjS3yOCtwuBAJ1mA== 5, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 5, 27 -- To: {microscopy-at-microscopy.com} 5, 27 -- X-OriginalArrivalTime: 02 Apr 2009 15:15:43.0683 (UTC) FILETIME=[E43B3130:01C9B3A5] 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n32FFni5014879 ==============================End of - Headers==============================
Please allow me to introduce myself. My name is Lisa Chen from Glosun Tech LLC.
We are currently building our thin film solar cell lab which requires a TEM (Transmission Electron Microscope) to do the quality control. If you have an used TEM that you wish to give away, please let us know, our email is lisa-at-glosuntech.com. We will be glad to come and give it a new home. We will pay all shipping and packing costs.
Glosun Tech LLC is a start-up small business company, which manufactures thin film CIGS and dye sensitized solar cells. We also provide accurate performance and reliability testing for photovoltaic materials and devices including Si, GaAs, CdTe, CIGS, dye-sensitized, and organic solar cells.
We really appreciate your help and time.
Best regards,
Lisa Chen
Glosuntech LLC
==============================Original Headers============================== 12, 31 -- From lisa-at-glosuntech.com Thu Apr 2 14:50:39 2009 12, 31 -- Received: from mta4.brinkster.com (mta4.brinkster.com [65.182.109.73]) 12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n32Joc2F010643 12, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 14:50:39 -0500 12, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id 46EEB5ED962 12, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 15:50:36 -0400 (EDT) 12, 31 -- X-Virus-Scanned: amavisd-new at 12, 31 -- X-Spam-Flag: NO 12, 31 -- X-Spam-Score: -2.499 12, 31 -- X-Spam-Level: 12, 31 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=5 12, 31 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1] 12, 31 -- Received: from mta4.brinkster.com ([127.0.0.1]) 12, 31 -- by localhost (mta4.brinkster.com [127.0.0.1]) (amavisd-new, port 10024) 12, 31 -- with ESMTP id lPZssg+8Yjr2 for {Microscopy-at-Microscopy.Com} ; 12, 31 -- Thu, 2 Apr 2009 15:50:31 -0400 (EDT) 12, 31 -- Received: from mail10a.brinkster.com (mail10a.brinkster.com [10.0.6.217]) 12, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id 1BD5C5EDBB8 12, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Apr 2009 15:50:31 -0400 (EDT) 12, 31 -- Date: Thu, 2 Apr 2009 15:50:32 -0400 (EDT) 12, 31 -- From: lisa-at-glosuntech.com 12, 31 -- To: Microscopy-at-Microscopy.Com 12, 31 -- Message-ID: {26215955.131111238701832717.JavaMail.root-at-mail10a.brinkster.com} 12, 31 -- In-Reply-To: {27147398.130841238701573423.JavaMail.root-at-mail10a.brinkster.com} 12, 31 -- Subject: Looking for an used TEM 12, 31 -- MIME-Version: 1.0 12, 31 -- Content-Type: text/plain; charset=utf-8 12, 31 -- Content-Transfer-Encoding: 7bit 12, 31 -- X-Originating-IP: [75.140.108.220] 12, 31 -- X-Mailer: Zimbra 5.0.8_GA_2463.RHEL4 (ZimbraWebClient - FF3.0 (Win)/5.0.8_GA_2462.RHEL4_64) ==============================End of - Headers==============================
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You might give these guys a call and see if they can repair the board. They specialize in component level repair. They keep the FAA's junk from the 60's running. SMH ELECTRONICS CO 508-291-7447
Usual disclaimers,
Tom Kaye
-----Original Message----- X-from: bart-at-cannonmicroprobe.com [mailto:bart-at-cannonmicroprobe.com] Sent: Thursday, April 02, 2009 3:53 PM To: tom-at-tomkaye.com
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To the knowing, We have a precipitation problem with mouse liver fixed by immersion with 2%PLP and then processed for LR Gold embedding (minus tannic acid and uranyl acetate)dehydrated in ETOH. It looks like traditional Pb pepper, but none was used. The attached jpeg is cut then immediately viewed (no labelling or staining). Any thoughts?
M Delannoy
==============================Original Headers============================== 2, 27 -- From delannoy-at-jhmi.edu Fri Apr 3 14:30:35 2009 2, 27 -- Received: from ipex2.johnshopkins.edu (ipex2.johnshopkins.edu [162.129.8.151]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n33JUYcZ006899 2, 27 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 14:30:35 -0500 2, 27 -- X-IronPort-AV: E=Sophos;i="4.39,320,1235970000"; 2, 27 -- d="scan'208";a="177815969" 2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 2, 27 -- by ipex2.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 03 Apr 2009 15:30:33 -0400 2, 27 -- Received: from johnshopkins.edu ([10.181.31.211]) by jesmail.johnshopkins.edu 2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 2, 27 -- with ESMTP id {0KHJ00453I6Z6WK0-at-jesmail.johnshopkins.edu} for 2, 27 -- microscopy-at-microscopy.com; Fri, 03 Apr 2009 15:30:35 -0400 (EDT) 2, 27 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.59]) 2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Fri, 03 Apr 2009 15:30:35 -0400 2, 27 -- Date: Fri, 03 Apr 2009 15:30:35 -0400 2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 27 -- Subject: lr gold precip 2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 27 -- Message-id: {f6b6abccbe1.49d62b9b-at-johnshopkins.edu} 2, 27 -- MIME-version: 1.0 2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 2, 27 -- Content-type: text/plain; charset=us-ascii 2, 27 -- Content-language: en 2, 27 -- Content-transfer-encoding: 7BIT 2, 27 -- Content-disposition: inline 2, 27 -- X-Accept-Language: en 2, 27 -- Priority: normal ==============================End of - Headers==============================
I'm still having trouble trying to find where the ground wire (at least I think it's a ground wire) attaches to the bulb assembly of the HBO100 on my Photomik III. This thing might not even attach to the bulb assembly-but I can't remember.
I don't have access to a server with which to post images but if you contact me off list I can send you some .jpg images I made of the little beasty.
Even the Zeiss people are having a tough time with this one. All the Zeiss documentation shows a completely different set up from the one I have, lucky me.
Put on you thinking caps and please try to help me solve my puzzle.
Let this be a lesson to all-either put it back together while awaiting parts or take detailed notes. You never know when you have to quickly vacate a facility and then have your brain vacate during the interim.
Thanks,
Paula the forgetful ;-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 13, 25 -- From vapatpxs-at-yahoo.com Fri Apr 3 15:54:48 2009 13, 25 -- Received: from n23b.bullet.mail.mud.yahoo.com (n23b.bullet.mail.mud.yahoo.com [68.142.206.142]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n33KslTZ023032 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Apr 2009 15:54:47 -0500 13, 25 -- Received: from [68.142.200.227] by n23.bullet.mail.mud.yahoo.com with NNFMP; 03 Apr 2009 20:54:47 -0000 13, 25 -- Received: from [68.142.201.243] by t8.bullet.mud.yahoo.com with NNFMP; 03 Apr 2009 20:54:47 -0000 13, 25 -- Received: from [127.0.0.1] by omp404.mail.mud.yahoo.com with NNFMP; 03 Apr 2009 20:54:47 -0000 13, 25 -- X-Yahoo-Newman-Property: ymail-3 13, 25 -- X-Yahoo-Newman-Id: 273625.7841.bm-at-omp404.mail.mud.yahoo.com 13, 25 -- Received: (qmail 7730 invoked by uid 60001); 3 Apr 2009 20:54:46 -0000 13, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1238792086; bh=u5aPJqnN7bFp8By7x99KWHprApqHmJONBrbvrNGKFEI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=lRkxVAzd+BBTH4PSO//3HH7ar07PbzcXbvRu0O1Rpk/G7hbfe21qeH0W660REoT3euplPo+Db3tVm3cuQ8cNKYbWVq/T8Etqy11c0+yKBzD8o9IhaDGzGJaxomdwuxmiLub/IcgTSzKYI5gNGuvrG2WLDdxxxrrTEtenaNMc9U8= 13, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 25 -- s=s1024; d=yahoo.com; 13, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; 13, 25 -- b=oNLwbpiknFnfEqqEq+x7eVVQ0eToUepBZmeiKifTL44sEqhhtsUaEOTFlU+iFMxt4JTsw1Bn+eyaSc+qEgoxvsnalAMZmDWMYbkEvWpzvjSWLCFs7DciHsSemiFUunFIUvLfOvhlGFnfbHelSMBMVnin4oe0zDY3ETDpNbCSzec=; 13, 25 -- Message-ID: {753201.7507.qm-at-web46112.mail.sp1.yahoo.com} 13, 25 -- X-YMail-OSG: C2Nt_UwVM1nJfGZuQ8rdPHHn8DWDBJWXszPqGqIiZyHkii4Nwmk15P.NpsYYuVOrFjFE78GUZpCY6D1w6jCLZOvxivYtQ1Js_wjaJs.MeN_Vtb7xKaJc38TncWVlq3jJQhZ.bzqrtDkJxNLvN02WeuCboYm1mXhRKknZs_N0sG8Byihtd6pkbJXTP.GJBJc9cylnP3S6xHBbk9IAYoIUjRHylxnzVNPTNc2BmaansgLOmjQ2dWBXh3rCIC4JEx0cLpqGs479xE9P5LrNuFg- 13, 25 -- Received: from [132.239.85.200] by web46112.mail.sp1.yahoo.com via HTTP; Fri, 03 Apr 2009 13:54:46 PDT 13, 25 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/0.7.289.1 13, 25 -- Date: Fri, 3 Apr 2009 13:54:46 -0700 (PDT) 13, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 13, 25 -- Subject: Zeiss Photomik III 13, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
I have a question about TEM digital cameras. Are they capable of displaying live FFT images for stigmation and focusing? Do this capability come usually as option or built in basic package?
Thank you,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
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Email: frankehrenfeld-at-iatl.com Name: Frank Ehrenfeld
Organization: IATL
Title-Subject: [Filtered] Used EDS
Question: We have two Kevex Delta Class EDS. We are looking for used EDS of same vintage or other used EDXA systems for our two TEMs. Please respond to the email above.
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Email: mdelann1-at-jhmi.edu Name: Michael Delannoy
Organization: JHMI Microscope Facility
Title-Subject: [Filtered] LR Gold precipitate
Question: To the knowing, We have a precipitation problem with mouse liver fixed by immersion with 2%PLP and then processed for LR Gold embedding (minus tannic acid and uranyl acetate)dehydrated in ETOH. It looks like traditional Pb pepper, but none was used. The attached jpeg is cut then immediately viewed (no labelling or staining). Any thoughts?
Frank Numonyx recently warehoused a 30 square mm Noran detector that fit a JEOL2010. Since IATL is a commercial lab, you would need to bid for it. Donations are for universties only. Contact Caroline Ayre at 4087652348 . Sent from my iPhone
On Apr 3, 2009, at 4:42 PM, frankehrenfeld-at-iatl.com wrote:
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I would like to find out about commercial companies and their services for TEM work: Types of samples, pricing, conditions etc. I mean companies who would accept samples from anyone who need a TEM work performed on their sample(s).
I have one critical question in mind: Given the fact that success rate in bulk sample preparation for TEM is not 100%, can a commercial company guaranty that they will produce TEM results from my sample? Especially, when I have a limited amount of original sample to begin with.
Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
I have another question regarding TEM work. I want to know how people organize TEM work at university laboratories in other parts of the World. I have completed my graduate study in United States.
In United States, we were given instructions to operate various sample prep tools and TEM itself. And we, as graduate students, were expected to prepare our samples and carry out our own TEM study. Which means we make few mistakes and break few samples before we could finally prepare a meaningful TEM sample.
I want to know how things are done in other parts of the World. Any pros and cons when compared to American system?
Thanks a bunch, Ayten.
=========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
I know that all digital TEM-Cameras from TVIPS Germany, and - I think, all - Gatan cameras have FFT capabilities, built into their own software, either live or with some delay (fractions of a second, or a second), depending on the speed of the camera, and the PC etc. best regards, Reinhard
--
PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 6, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Sun Apr 5 15:30:55 2009 6, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n35KUoG0018085 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 15:30:54 -0500 6, 25 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1]) 6, 25 -- by localhost (Postfix) with SMTP id 1042AABA34 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 22:30:57 +0200 (CEST) 6, 25 -- Received: from gwsmtp2.uni-regensburg.de (rrzgw6.rz.uni-regensburg.de [132.199.4.79]) 6, 25 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id 09176ABA08 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Sun, 5 Apr 2009 22:30:57 +0200 (CEST) 6, 25 -- Received: from uni-regensburg-3-MTA by gwsmtp2.uni-regensburg.de 6, 25 -- with Novell_GroupWise; Sun, 05 Apr 2009 22:30:47 +0200 6, 25 -- Message-Id: {49D93114020000540000CD7B-at-gwsmtp2.uni-regensburg.de} 6, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0 6, 25 -- Date: Sun, 05 Apr 2009 22:30:44 +0200 6, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 6, 25 -- To: {Microscopy-at-Microscopy.Com} 6, 25 -- Subject: FFT with digital TEM camera 6, 25 -- References: {200904032256.n33Mup7a021612-at-ns.microscopy.com} 6, 25 -- In-Reply-To: {200904032256.n33Mup7a021612-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=US-ASCII 6, 25 -- Content-Disposition: inline 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n35KUoG0018085 ==============================End of - Headers==============================
New YorkMicroscopical Society One Prospect Village Plaza (66F Mount Prospect Avenue) Clifton, NJ 07013 Bernard Friedman Memorial Workshop
Polarized Light Microscopy May 2, 9, 16 & 23, 2009
An advanced course on polarized light microscopy which will cover the following topics: The nature of polarized light The origin and interpretation of interference colors Birefringence and crystal orientation, The Indicatrix Compensation and variable compensators Interference figures and their interpretation
The workshop will consist of four Consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch formally of Leica, Inc., Mary McCann of McCann Imaging, John Reffner of Smiths Detection and N.Y.M.S. Instructor Don O'Leary.
WHEN: May 2, 9, 16 & 23, 2009 from 10 A.M. to 4 P.M.
COST: $425 for N.Y.M.S. members, $455 for non-members (includes membership). Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants. Return form to Don O'Leary, 10 Sampson Street, Unit 113, Saddle Brook, NJ 07663 FURTHER INFORMATION: Call D. O'Leary (201)368-8849 e-mail dkoleary-at-verizon.net
PLEASE POST -------------------------------------------------------------------------- Registration Form Polarized Light Microscopy
Thanks to all who have responded to my previous inquiries.
Some university laboratories have one or two designated TEM operators who operate TEM for everyone in some parts of the world. Commercial TEM laboratories generally have specific areas of expertise.
Is there any university laboratory (a core facility) out there who has a technician to do TEM sample preparation for graduate students? Please, let me know if there is such a model out there? Given the differences in sample preparation techniques of different kinds of samples, I wonder how they do things? Do they have few technicians, one for each kind of sample group (metals, biological samples, ceramics etc.)?
Personally, I think there are numerous benefits to have graduate students do their own sample preparation. Still, I like to hear about other universities where they do things differently.
Thanks, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Sat, Apr 4, 2009 at 1:25 PM, {celikaktas-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Colleagues, } } I have another question regarding TEM work. I want to know how people } organize TEM work at university laboratories in other parts of the } World. I have completed my graduate study in United States. } } In United States, we were given instructions to operate various sample } prep tools and TEM itself. And we, as graduate students, were expected } to prepare our samples and carry out our own TEM study. Which means we } make few mistakes and break few samples before we could finally } prepare a meaningful TEM sample. } } I want to know how things are done in other parts of the World. Any } pros and cons when compared to American system? } } Thanks a bunch, } Ayten. } }
I have used Fuji Imaging Plates for electron diffraction study and loved it.
X-from technical point of view, imaging plates have linear response and higher dynamic range compared to traditional photo films. This is really important especially for phase retrieval calculations.
Another plus is that one does not need to mess up with chemicals to develop micrographs.
Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Thu, Apr 2, 2009 at 12:18 AM, {wzhe-at-laurentian.ca} wrote: } } Email: wzhe-at-laurentian.ca } Name: William } } Organization: Luarentian Univ } } Title-Subject: [Filtered] Imaging plates for TEM Photo } } Question: Dear there, } } We want to use Fuji imaging plates in TEM photo work and read some } recommend on product FDL-5000 plate. If someone could comment on this } imaging plate } } 1. What performance looks like in TEM photos? } 2. Any alternate we can try, because this plate is quite expensive to } start with, ~US$4000 for a 16 pieces/pack ? } } Thanks very much in advance, } } William } } Â Login Host: 142.51.53.72 }
} Is there any university laboratory (a core facility) out there who has } a technician to do TEM sample preparation for graduate students? } Please, let me know if there is such a model out there? Given the } differences in sample preparation techniques of different kinds of } samples, I wonder how they do things? Do they have few technicians, } one for each kind of sample group (metals, biological samples, } ceramics etc.)? } } Personally, I think there are numerous benefits to have graduate } students do their own sample preparation. Still, I like to hear about } other universities where they do things differently.
The graduate students here are expected to learn all techniques required for the project that they undertake, EM is no exception- sometimes it is impossible for them to always carry out every step of a process (for example if the facilities to carry out a procedure are not available here). In these cases every possible attempt is made for them to have practical experience of carrying out the process once- e.g. a trip to a collaborating University that is carrying out the procedure.
In terms of EM, the students here have all the facilities they need for their projects, and they undertake all aspects of their work themselves. In the past, when we used film for TEM, I would process their film, but they would make their own prints from the negatives- that was the only exception. But they all left with a full understanding of how the development was done.
If EM is only a small side branch of a project, I can see why it is not so important for the student to spend a lot of time learning all the stages of preparation, but they should still understand them all, and preferably carry them out at least once by themselves.
The final stage of doctoral degrees here is the viva voce (similar to the thesis defence), and the examiners have the right to question the student on all aspects of the work they have submitted. A thorough understanding is expected, and having experience in all techniques they include in their thesis is the best way of achieving this.
Having technicians carry out students' sample preparation is not helping them gain experience, which is what they are here to do. There are also not enough technicians to do all the students preparation work, and it would not be economically sensible if there were.
In our Unit, even post-docs do almost all of their own EM preparation- they want to be sure that it has been done exactly the same each time, and up to their own standards. They will spend so much of their own time examining their grids in the EM, analysing the images and quantifying the results, that the don't want to risk wasting their time if mistakes happen- this way, all mistakes must be their own!
Regards,
Ben Micklem
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 {http://mrcanu.pharm.ox.ac.uk/}
==============================Original Headers============================== 13, 29 -- From ben.micklem-at-pharm.ox.ac.uk Mon Apr 6 05:07:23 2009 13, 29 -- Received: from relay7.mail.ox.ac.uk (relay7.mail.ox.ac.uk [129.67.1.167]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n36A7MEs000621 13, 29 -- for {microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 05:07:23 -0500 13, 29 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207]) 13, 29 -- by relay7.mail.ox.ac.uk with esmtp (Exim 4.69) 13, 29 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 13, 29 -- id 1Lqljc-0003Jj-Ox 13, 29 -- for microscopy-at-microscopy.com; Mon, 06 Apr 2009 11:07:20 +0100 13, 29 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) 13, 29 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 13, 29 -- (Exim 4.69) 13, 29 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 13, 29 -- id 1Lqljc-0004E4-4k 13, 29 -- for microscopy-at-microscopy.com; Mon, 06 Apr 2009 11:07:20 +0100 13, 29 -- Message-ID: {49D9D458.9090402-at-pharm.ox.ac.uk} 13, 29 -- Date: Mon, 06 Apr 2009 11:07:20 +0100 13, 29 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 13, 29 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302) 13, 29 -- MIME-Version: 1.0 13, 29 -- To: microscopy-at-microscopy.com 13, 29 -- Subject: Re: [Microscopy] TEM sample preparation? Re:TEM work at Non-American 13, 29 -- Universities 13, 29 -- References: {200904060931.n369VLN8030462-at-ns.microscopy.com} 13, 29 -- In-Reply-To: {200904060931.n369VLN8030462-at-ns.microscopy.com} 13, 29 -- X-Enigmail-Version: 0.95.7 13, 29 -- Content-Type: text/plain; charset=ISO-8859-1 13, 29 -- Content-Transfer-Encoding: 7bit 13, 29 -- X-Oxford-Username: phar0293 ==============================End of - Headers==============================
I don't have a TEM here and admittedly, most of our SEM preps are pretty straightforward mounts of solid, never-alive material (e.g. ceramics) but I like to show our students how to do the preparation work and urge them to develop a good understanding of the whys. Then they have to do their own specimen mounting and preparation. They will leave hear and may end up doing further research and/or teaching others so they should have a working knowledge of how things are done, if for no other reason than to understand what they read about other people's research and to recognize if it has been done well or poorly.
Ron L
-----Original Message----- X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] Sent: Monday, April 06, 2009 5:20 AM To: lherault-at-bu.edu
Thanks to all who have responded to my previous inquiries.
Some university laboratories have one or two designated TEM operators who operate TEM for everyone in some parts of the world. Commercial TEM laboratories generally have specific areas of expertise.
Is there any university laboratory (a core facility) out there who has a technician to do TEM sample preparation for graduate students? Please, let me know if there is such a model out there? Given the differences in sample preparation techniques of different kinds of samples, I wonder how they do things? Do they have few technicians, one for each kind of sample group (metals, biological samples, ceramics etc.)?
Personally, I think there are numerous benefits to have graduate students do their own sample preparation. Still, I like to hear about other universities where they do things differently.
Thanks, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Sat, Apr 4, 2009 at 1:25 PM, {celikaktas-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Colleagues, } } I have another question regarding TEM work. I want to know how people } organize TEM work at university laboratories in other parts of the } World. I have completed my graduate study in United States. } } In United States, we were given instructions to operate various sample } prep tools and TEM itself. And we, as graduate students, were expected } to prepare our samples and carry out our own TEM study. Which means we } make few mistakes and break few samples before we could finally } prepare a meaningful TEM sample. } } I want to know how things are done in other parts of the World. Any } pros and cons when compared to American system? } } Thanks a bunch, } Ayten. } }
In the microscopy centre here, while we have a couple of SEMs, a confocal, several fluorescence microscopes, microtomes, etc., it's just me and my technical officer (who also has a cell biology-oriented PhD). Students and postdocs should learn to do their own preparations - how will they know what to do/how to advise their students when they start their own labs? And what if they are hired partly on their cell biology/microscopy skills as seen in a publication?
After all, you don't have a centre where everyone goes to get their gels or PCRs run by someone else, or to get their constructs made, do you (or maybe you do - not here, anyway)? Here, the lab techs also have to learn microscopy from go to whoa, and unless it's a collaboration, all the permanent scientists do, too. It's good for them.... they finally realise how much work and experience and TIME!! goes in to "taking that picture" - and they learn something new, too.
It wasn't like that when I arrived - people were used to handing material in at the door and getting an image out, and while was some grumbling at first, the switch has meant the instruments get used more - seemed crazy that there was so much equipment used about 5% of the time - now it's more like 50-70%, with some, like the confocal, used all day most days.
So, even in a non-university lab, which we are, we still do things this way.
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 6/04/09 8:26 PM, "celikaktas-at-gmail.com" {celikaktas-at-gmail.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks to all who have responded to my previous inquiries. } } Some university laboratories have one or two designated TEM operators } who operate TEM for everyone in some parts of the world. Commercial } TEM laboratories generally have specific areas of expertise. } } Is there any university laboratory (a core facility) out there who has } a technician to do TEM sample preparation for graduate students? } Please, let me know if there is such a model out there? Given the } differences in sample preparation techniques of different kinds of } samples, I wonder how they do things? Do they have few technicians, } one for each kind of sample group (metals, biological samples, } ceramics etc.)? } } Personally, I think there are numerous benefits to have graduate } students do their own sample preparation. Still, I like to hear about } other universities where they do things differently. } } } Thanks, } Ayten. } } -- } =========================== } Ayten Celik-Aktas, PhD } Ankara University } Electron Microscopy Laboratory } Ankara, Turkey } =========================== } } } On Sat, Apr 4, 2009 at 1:25 PM, {celikaktas-at-gmail.com} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Colleagues, } } } } I have another question regarding TEM work. I want to know how people } } organize TEM work at university laboratories in other parts of the } } World. I have completed my graduate study in United States. } } } } In United States, we were given instructions to operate various sample } } prep tools and TEM itself. And we, as graduate students, were expected } } to prepare our samples and carry out our own TEM study. Which means we } } make few mistakes and break few samples before we could finally } } prepare a meaningful TEM sample. } } } } I want to know how things are done in other parts of the World. Any } } pros and cons when compared to American system? } } } } Thanks a bunch, } } Ayten. } } } } } } } ==============================Original Headers============================== } 10, 32 -- From celikaktas-at-gmail.com Mon Apr 6 04:12:53 2009 } 10, 32 -- Received: from mail-fx0-f166.google.com (mail-fx0-f166.google.com } [209.85.220.166]) } 10, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n369Cqmd003716 } 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Apr 2009 04:12:53 -0500 } 10, 32 -- Received: by fxm10 with SMTP id 10so1956337fxm.18 } 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Apr 2009 02:12:51 } -0700 (PDT) } 10, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 32 -- d=gmail.com; s=gamma; } 10, 32 -- } h=domainkey-signature:mime-version:received:date:message-id:subject } 10, 32 -- :from:to:content-type:content-transfer-encoding; } 10, 32 -- bh=hIGF2MYnI1a/nY1D6OydWXjuizPhlwmi5YfHHrdCN7I=; } 10, 32 -- } b=Gxxh7qNx8lMd9g/K/cqboF511QjbHW0el1ROLR1f3DsYsA2tXiJm/5b87k98Xaudal } 10, 32 -- } LpX/djODW7NMj1Z803dFehOyBd3rsF7ficPdg29523/5v/rhn3Szd3+2+qIKYzS7vzBv } 10, 32 -- OygtC/EsxD/ws3/OjdOIfuqSuEu71LYYz2Bv0= } 10, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 10, 32 -- d=gmail.com; s=gamma; } 10, 32 -- h=mime-version:date:message-id:subject:from:to:content-type } 10, 32 -- :content-transfer-encoding; } 10, 32 -- } b=mTr7oN5LSIDLqQn2okI58kWsTE0EWUBDv3zMXoH5sxrlnwBBKiykqyQtfk7wwCT2rX } 10, 32 -- } En2LR8UAJnLQyZ+S/aRPS1VlqX7OA+saZn16IItkHAbACR9rmlJY3nJP4FJ8CeUaoH08 } 10, 32 -- RxzCSPY+b1CyGiw7Oc6pE9J7JLPr+tPD/IIwc= } 10, 32 -- MIME-Version: 1.0 } 10, 32 -- Received: by 10.103.137.12 with SMTP id } p12mr1879669mun.94.1239009171608; Mon, } 10, 32 -- 06 Apr 2009 02:12:51 -0700 (PDT) } 10, 32 -- Date: Mon, 6 Apr 2009 12:12:51 +0300 } 10, 32 -- Message-ID: } {1075c5c10904060212n63a443f4n701667d38fe5430-at-mail.gmail.com} } 10, 32 -- Subject: TEM sample preparation? Re:TEM work at Non-American } Universities } 10, 32 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} } 10, 32 -- To: microscopy {Microscopy-at-microscopy.com} } 10, 32 -- Content-Type: text/plain; charset=UTF-8 } 10, 32 -- Content-Transfer-Encoding: 8bit } 10, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n369Cqmd003716 } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ahmad_ds-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ahmad_ds-at-yahoo.com Name: Ahmad Ashkhaibi
Organization: BAU
Title-Subject: [Filtered] Oval TEM Beam
Question: I've got an oval beam as I started my TEM...and it get's a line shape as I increase the intensity what seems to be the problem? I've tried to adjust the astigmatism, but didn't work out.
You haven't given much detail so it could be several things.
For instance have you just changed the filament recently or done any other work in the electron gun or condenser area? What happens if you adjust focus of the condenser lens from underfocus to overfocus - does the direction of the oval shape change? Have you tried checking the complete alignment of the condenser system eg gun tilt, gun shift, movable condenser alignment? Finally I apologise for asking but you say you have adjusted the astigmatism - I assume you mean the condenser astigmatism?
If this has happened after a filament change then it could be a badly positioned filament or defective one. It could even be movement of the gun or condenser aperture.
Other possibilities might include some form of wobbler or scan system inadvertently switched on.
It might be useful to know what TEM you are using, as well.
Good luck
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: ahmad_ds-at-yahoo.com
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Email: parmiterd-at-mail.nih.gov Name: David Parmiter
Organization: SAIC-Frederick
Title-Subject: [Filtered] Senior Scientist Position
Question: To all -
We are looking for a Senior Scientist to run the Electron Microscopy Facility at SAIC-Frederick, a contractor for the National Cancer Institute. For all applications or inquiries, please contact the individual mentioned at the bottom. Thanks!
Position description and requirements:
SAIC-Frederick, Inc., a subsidiary of SAIC, develops and applies advanced technologies to meet the most urgent and challenging research and development needs of the National Cancer Institute, other government agencies, and the nation. We are the prime contractor for the National Cancer Institute at Frederick, one of 38 Federally Funded Research and Development Centers and the only such national laboratory devoted exclusively to biomedical research.
We are currently recruiting for a Senior Scientist (128940) at SAIC-Frederick. This scientist will manage the staff and operations of the electron microscopy (EM) laboratory at SAIC-Frederick. Specific duties include (i) managerial oversight of the EM laboratoryís core services (ii) interface with intra- and extramural investigators to provide EM characterization support (iii) methods development and research using techniques such as energy dispersive x-ray spectroscopy (EDS), cryogenics, focused ion beam ablation, 3D tomography on biological samples and (iv) interpretation of results and preparation of written reports. He/she will also work with an interdisciplinary team of scientists to characterize nanomaterials intended for cancer therapeutics and diagnostics.
Possession of a doctoral degree from an accredited college/ university in a field related to Chemistry, Biology, Material Science, Engineering or Physics. Foreign educated candidates who have completed part or all of their education outside of the United States must have their foreign education evaluated by an SAIC-approved accrediting organization to assure that it has met the equivalency of the qualifications of degree work in the United States. In addition to the educational requirements, a minimum of five years of related experience. A minimum of three years using electron microscopy (TEM, SEM) on biological tissues. Experience with energy dispersive x-ray spectroscopy (EDS), cryogenics, focused ion beam ablation, and 3D tomography. This position is subject to obtaining a Public Trust Clearance.
Excellent compensation package accompanies our position. For immediate consideration, please apply online at our website: www.saic.com for position #128940. SAIC is an equal opportunity employer and values cultural diversity in the workplace.
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What you are describing is known as Condenser Astigmatism. On your microscope there will be at least two devices for correcting the astigmatism, one for the condenser system, one for the objective system and possibly one for the intermediate system.
I feel that when you say you have tried adjusting the astigmatism you have used the objective controls not the condenser.
Try the following
1. With the beam on adjust the second condenser (illumination or brightness on some instruments) to cross over, the smallest beam spot. 2. Increase the magnification to make the spot about 2 to 3cms across. 3. Decrease the filament heating until the beam breaks up into a spot and halo formation. 4. Adjust the illumination to focus this image as sharp as you can 5. Adjust each condenser stigmator in turn until the spot and halo image is at its sharpest. 6. Repeat 4 and 5 until you have no improvement. 7. Heat the filament to the level you require for your tasks.
Good luck
Steve
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com -----Original Message----- X-from: ahmad_ds-at-yahoo.com [mailto:ahmad_ds-at-yahoo.com] Sent: 07 April 2009 14:02 To: protrain-at-emcourses.com
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Email: ahmad_ds-at-yahoo.com Name: Ahmad Ashkhaibi
Organization: BAU
Title-Subject: [Filtered] Oval TEM Beam
Question: I've got an oval beam as I started my TEM...and it get's a line shape as I increase the intensity what seems to be the problem? I've tried to adjust the astigmatism, but didn't work out.
Hi all, How long can slides with sections be stored before they are used for immunolabeling? 6 months in the refrigerator? or longer? or is it a bad idea to wait? I usually cut sections then label the next day but someone here would like to store the slides for awhile if that is an okay practice.
Any advice would be greatly appreciated. thanks, Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
*************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.com
==============================Original Headers============================== 14, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 10:29:51 2009 14, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FToR8012316 14, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:29:50 -0500 14, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 14, 19 -- (authenticated user beth-at-plantbio.uga.edu) 14, 19 -- by dogwood.plantbio.uga.edu 14, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 14, 19 -- for microscopy-at-microscopy.com; 14, 19 -- Tue, 7 Apr 2009 11:29:46 -0400 14, 19 -- Message-Id: {13CA0374-0445-42E7-8DBB-62B77A025359-at-plantbio.uga.edu} 14, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 14, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 14, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 19 -- Content-Transfer-Encoding: 7bit 14, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) 14, 19 -- Subject: storing slides before immunolabeling 14, 19 -- Date: Tue, 7 Apr 2009 11:29:21 -0400 14, 19 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Dear Listers I have a complete set of manuals (installation, service and user) for a Hitachi S-570 SEM available free - if this is of interest to you please contact me off list
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
==============================Original Headers============================== 7, 26 -- From rjharris-at-uwo.ca Tue Apr 7 10:48:28 2009 7, 26 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FmRld007967 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:48:28 -0500 7, 26 -- MIME-version: 1.0 7, 26 -- Content-type: text/plain; charset=iso-8859-1 7, 26 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 7, 26 -- by zeppo.mail.uwo.pri 7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 26 -- with ESMTP id {0KHQ0085WMKPTYI0-at-zeppo.mail.uwo.pri} for 7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT) 7, 26 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 7, 26 -- by zeppo.mail.uwo.pri 7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 26 -- with ESMTPSA id {0KHQ00MXHMKPR650-at-zeppo.mail.uwo.pri} for 7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT) 7, 26 -- From: Richard Harris {rjharris-at-uwo.ca} 7, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com} 7, 26 -- Subject: Manuals for S-570 SEM 7, 26 -- Date: Tue, 07 Apr 2009 11:48:26 -0400 7, 26 -- Message-id: {002601c9b798$4a45f320$ded1d960$-at-ca} 7, 26 -- X-Mailer: Microsoft Office Outlook 12.0 7, 26 -- Content-language: en-us 7, 26 -- Thread-index: Acm3mEoiKth8qgRsTZSHCx9a+yeIWw== 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37FmRld007967 ==============================End of - Headers==============================
PS - I forgot to say that the tissue is embedded in LR White.
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
*************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.com
==============================Original Headers============================== 11, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 11:02:11 2009 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G29Kn022252 11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:02:10 -0500 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 11, 19 -- (authenticated user beth-at-plantbio.uga.edu) 11, 19 -- by dogwood.plantbio.uga.edu 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 11, 19 -- for microscopy-at-microscopy.com; 11, 19 -- Tue, 7 Apr 2009 12:02:05 -0400 11, 19 -- Message-Id: {467F2271-FDE6-4830-91B7-95CD62EBC607-at-plantbio.uga.edu} 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 19 -- Subject: storing slides - section info 11, 19 -- Date: Tue, 7 Apr 2009 12:01:40 -0400 11, 19 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
This comes under "Looking for small things in large places".
One of our clients has determined the presence, but not the location, of bacteria in mushroom fruiting bodies. The mushrooms are about the size of the common white mushrooms you buy at the grocery store. He wants to know where the bugs be.
We have tried breaking pieces off the mushroom and viewing them in an environmental SEM and, while we found all sorts of neat reproductive stuff, no bacteria were found. To search systematically through an entire fruiting body with no clue as to where the wee beasties might be can be done, but it's going to be expensive and time-consuming, especially since we don't know their physical appearance.
TEM seems completely impractical for this for obvious reasons (if not, please enlighten me).
So, if anyone has ideas on looking for needles in a fungal haystack, I'm all ears. Real ears, not wood ears.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 9, 27 -- From TindallR-at-missouri.edu Tue Apr 7 11:09:39 2009 9, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G9cl7000497 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:09:39 -0500 9, 27 -- X-IronPort-Anti-Spam-Filtered: true 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEALkX20nRauUo/2dsb2JhbAC/VAEJhwGITYJKgTMG 9, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 9, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 11:09:36 -0500 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 27 -- Tue, 7 Apr 2009 11:09:35 -0500 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="us-ascii" 9, 27 -- Subject: SEM: Locating bacteria in fungi 9, 27 -- Date: Tue, 7 Apr 2009 11:09:35 -0500 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FC9-at-UM-XMAIL08.um.umsystem.edu} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: SEM: Locating bacteria in fungi 9, 27 -- Thread-Index: Acm3mz5qeov9y91NQHG6WZ82emeIxA== 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 27 -- To: {microscopy-at-microscopy.com} 9, 27 -- X-OriginalArrivalTime: 07 Apr 2009 16:09:35.0394 (UTC) FILETIME=[3E8BE820:01C9B79B] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37G9cl7000497 ==============================End of - Headers==============================
What type of sections are you talking about? Resin, paraffin, or cryo? I store resin and paraffin for years with no apparent problem. I have stored cryo for months at -80 but you risk desiccation or other problems. Usually it is obvious when the cryo morphology has deteriorated. Good luck.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Tuesday, April 07, 2009 10:31 AM To: Phillips, Thomas E.
Hi all, How long can slides with sections be stored before they are used for immunolabeling? 6 months in the refrigerator? or longer? or is it a bad idea to wait? I usually cut sections then label the next day but someone here would like to store the slides for awhile if that is an okay practice.
Any advice would be greatly appreciated. thanks, Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
************************************************************************ *** The Friends of the Marine Institute - Join Today! www.friendsofugami.com
==============================Original Headers============================== 14, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 10:29:51 2009 14, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 14, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FToR8012316 14, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:29:50 -0500 14, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 14, 19 -- (authenticated user beth-at-plantbio.uga.edu) 14, 19 -- by dogwood.plantbio.uga.edu 14, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 14, 19 -- for microscopy-at-microscopy.com; 14, 19 -- Tue, 7 Apr 2009 11:29:46 -0400 14, 19 -- Message-Id: {13CA0374-0445-42E7-8DBB-62B77A025359-at-plantbio.uga.edu} 14, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 14, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 14, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 19 -- Content-Transfer-Encoding: 7bit 14, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) 14, 19 -- Subject: storing slides before immunolabeling 14, 19 -- Date: Tue, 7 Apr 2009 11:29:21 -0400 14, 19 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 24, 29 -- From PhillipsT-at-missouri.edu Tue Apr 7 11:47:45 2009 24, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37GliQU019253 24, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:47:44 -0500 24, 29 -- X-IronPort-Anti-Spam-Filtered: true 24, 29 -- X-IronPort-Anti-Spam-Result: ApoEACcg20nRauUo/2dsb2JhbAC/XgEJhwyITQGCSYEzBoc/ 24, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 24, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 11:47:44 -0500 24, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 24, 29 -- Tue, 7 Apr 2009 11:47:43 -0500 24, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 29 -- Content-class: urn:content-classes:message 24, 29 -- MIME-Version: 1.0 24, 29 -- Content-Type: text/plain; 24, 29 -- charset="us-ascii" 24, 29 -- Subject: RE: [Microscopy] storing slides before immunolabeling 24, 29 -- Date: Tue, 7 Apr 2009 11:47:41 -0500 24, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD064EA3E2-at-UM-XMAIL06.um.umsystem.edu} 24, 29 -- In-Reply-To: {200904071531.n37FVEw3013445-at-ns.microscopy.com} 24, 29 -- X-MS-Has-Attach: 24, 29 -- X-MS-TNEF-Correlator: 24, 29 -- Thread-Topic: [Microscopy] storing slides before immunolabeling 24, 29 -- Thread-Index: Acm3leW6d1zZSsI0QJOluEAPq3fKmgAChohA 24, 29 -- References: {200904071531.n37FVEw3013445-at-ns.microscopy.com} 24, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 24, 29 -- To: {beth-at-plantbio.uga.edu} , {Microscopy-at-microscopy.com} 24, 29 -- X-OriginalArrivalTime: 07 Apr 2009 16:47:43.0908 (UTC) FILETIME=[929B6E40:01C9B7A0] 24, 29 -- Content-Transfer-Encoding: 8bit 24, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37GliQU019253 ==============================End of - Headers==============================
Tissue should be relatively stable in epoxy even at room temperature. Consider Kristina Micheva's (Stephen Smith Lab) Array Tomography technique where LR White sections are repeatedly immunolabeled and each label is eluted with .2M NaOH and .1% SDS before the next label is applied. Of course, there is always an antigen that is the exception. Larry
beth-at-plantbio.uga.edu wrote: } Hi all, How long can slides with sections be stored before they are used for immunolabeling? 6 months in the refrigerator? or longer? or is it a bad idea to wait? I usually cut sections then label the next day but someone here would like to store the slides for awhile if that is an okay practice.
Any advice would be greatly appreciated. thanks, Beth } } PS - I forgot to say that the tissue is embedded in LR White. } } ********************************************************************** } Beth Richardson } Electron Microscopy Lab Coordinator } Plant Biology Department } University of Georgia } Athens, GA 30602-7271 } } http://www.plantbio.uga.edu/emlab/ } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } } "Between the two evils, } I always pick the one I never tried before". Mae West (1893-1980) } ******************************************************************* } } "And it's only the giving that makes you what you are". } Wond'ring Aloud, Jethro Tull (Aqualung) } } *************************************************************************** } The Friends of the Marine Institute - Join Today! } www.friendsofugami.com } } } } } } ==============================Original Headers============================== } 11, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 11:02:11 2009 } 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) } 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G29Kn022252 } 11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:02:10 -0500 } 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) } 11, 19 -- (authenticated user beth-at-plantbio.uga.edu) } 11, 19 -- by dogwood.plantbio.uga.edu } 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) } 11, 19 -- for microscopy-at-microscopy.com; } 11, 19 -- Tue, 7 Apr 2009 12:02:05 -0400 } 11, 19 -- Message-Id: {467F2271-FDE6-4830-91B7-95CD62EBC607-at-plantbio.uga.edu} } 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 11, 19 -- Content-Transfer-Encoding: 7bit } 11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 11, 19 -- Subject: storing slides - section info } 11, 19 -- Date: Tue, 7 Apr 2009 12:01:40 -0400 } 11, 19 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers============================== }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
On Apr 7, 2009, at 9:09 AM, TindallR-at-missouri.edu wrote:
} This comes under "Looking for small things in large places". } } One of our clients has determined the presence, but not the } location, of } bacteria in mushroom fruiting bodies. The mushrooms are about the } size } of the common white mushrooms you buy at the grocery store. He } wants to } know where the bugs be. } } We have tried breaking pieces off the mushroom and viewing them in an } environmental SEM and, while we found all sorts of neat reproductive } stuff, no bacteria were found. To search systematically through an } entire fruiting body with no clue as to where the wee beasties might } be } can be done, but it's going to be expensive and time-consuming, } especially since we don't know their physical appearance. } } TEM seems completely impractical for this for obvious reasons (if not, } please enlighten me). } } So, if anyone has ideas on looking for needles in a fungal haystack, } I'm } all ears. Real ears, not wood ears.
Dear Randy, I would try fluorescence light microscopy to scan large volumes of the shrooms, then look at the areas of interest by SEM if necessary. Of course, this supposes that your client can label the bacteria specifically with a fluorescent probe. Since the presence of the bacteria was determined, perhaps the process that showed the presence of bacteria could be used to provide a suitable label--it all depends on how the bacteria were identified. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Tue Apr 7 13:11:41 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37IBerV017448 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 13:11:41 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id B0DC166E188D 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:11:39 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-195.caltech.edu (DHCP-19-195.caltech.edu [131.215.19.195]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id BA10666E4191 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:11:38 -0700 (PDT) 6, 22 -- Message-Id: {315BC34D-3B3A-4127-BD09-4741B868798F-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200904071609.n37G9kKi000827-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] SEM: Locating bacteria in fungi 6, 22 -- Date: Tue, 7 Apr 2009 11:11:38 -0700 6, 22 -- References: {200904071609.n37G9kKi000827-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Thanks for all the suggestions on locating little bugs in big mushrooms. The consensus seems to be using paraffin sections with appropriate staining and/or fluorescence to located the general location, then EM'ing in on the critters.
That's what happens when you get in an electron rut, I guess. I forgot to think outside of it. Thanks to everyone for the ideas!
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 8, 27 -- From TindallR-at-missouri.edu Tue Apr 7 13:19:51 2009 8, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37IJnFe025199 8, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 13:19:50 -0500 8, 27 -- X-IronPort-Anti-Spam-Filtered: true 8, 27 -- X-IronPort-Anti-Spam-Result: ApoEAGw220nRauUp/2dsb2JhbAC/ZQEJhxmITYJIgTMG 8, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 8, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 13:19:49 -0500 8, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 27 -- Tue, 7 Apr 2009 13:19:49 -0500 8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 27 -- Content-class: urn:content-classes:message 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="us-ascii" 8, 27 -- Subject: Bacteria and fungi 8, 27 -- Date: Tue, 7 Apr 2009 13:19:48 -0500 8, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FD7-at-UM-XMAIL08.um.umsystem.edu} 8, 27 -- X-MS-Has-Attach: 8, 27 -- X-MS-TNEF-Correlator: 8, 27 -- Thread-Topic: Bacteria and fungi 8, 27 -- Thread-Index: Acm3rW+NpIvKBwX2QueRdSP2b71AqQ== 8, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 27 -- To: {microscopy-at-microscopy.com} 8, 27 -- X-OriginalArrivalTime: 07 Apr 2009 18:19:49.0203 (UTC) FILETIME=[6FF08A30:01C9B7AD] 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37IJnFe025199 ==============================End of - Headers==============================
Dear listers Thank you for your overwhelming support - the manuals have been spoken for
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935
-----Original Message----- X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca] Sent: Tuesday, April 07, 2009 11:56 AM To: rjharris-at-uwo.ca
Dear Listers I have a complete set of manuals (installation, service and user) for a Hitachi S-570 SEM available free - if this is of interest to you please contact me off list
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935
==============================Original Headers============================== 7, 26 -- From rjharris-at-uwo.ca Tue Apr 7 10:48:28 2009 7, 26 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37FmRld007967 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 10:48:28 -0500 7, 26 -- MIME-version: 1.0 7, 26 -- Content-type: text/plain; charset=iso-8859-1 7, 26 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 7, 26 -- by zeppo.mail.uwo.pri 7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 26 -- with ESMTP id {0KHQ0085WMKPTYI0-at-zeppo.mail.uwo.pri} for 7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT) 7, 26 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 7, 26 -- by zeppo.mail.uwo.pri 7, 26 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 7, 26 -- with ESMTPSA id {0KHQ00MXHMKPR650-at-zeppo.mail.uwo.pri} for 7, 26 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 11:48:25 -0400 (EDT) 7, 26 -- From: Richard Harris {rjharris-at-uwo.ca} 7, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com} 7, 26 -- Subject: Manuals for S-570 SEM 7, 26 -- Date: Tue, 07 Apr 2009 11:48:26 -0400 7, 26 -- Message-id: {002601c9b798$4a45f320$ded1d960$-at-ca} 7, 26 -- X-Mailer: Microsoft Office Outlook 12.0 7, 26 -- Content-language: en-us 7, 26 -- Thread-index: Acm3mEoiKth8qgRsTZSHCx9a+yeIWw== 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37FmRld007967 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 29 -- From rjharris-at-uwo.ca Tue Apr 7 14:40:01 2009 18, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37Je1rH016051 18, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 14:40:01 -0500 18, 29 -- MIME-version: 1.0 18, 29 -- Content-type: text/plain; charset=iso-8859-1 18, 29 -- Received: from harpo.mail.uwo.pri (brutus.mail.uwo.pri [172.29.32.39]) 18, 29 -- by harpo.mail.uwo.pri 18, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 18, 29 -- with ESMTP id {0KHQ00K0MXAN18I0-at-harpo.mail.uwo.pri} for 18, 29 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 15:39:59 -0400 (EDT) 18, 29 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 18, 29 -- by harpo.mail.uwo.pri 18, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 18, 29 -- with ESMTPSA id {0KHQ00LEFXANWL00-at-harpo.mail.uwo.pri} for 18, 29 -- Microscopy-at-microscopy.com; Tue, 07 Apr 2009 15:39:59 -0400 (EDT) 18, 29 -- From: Richard Harris {rjharris-at-uwo.ca} 18, 29 -- To: rjharris-at-uwo.ca 18, 29 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 18, 29 -- References: {200904071556.n37Fu0dD020190-at-ns.microscopy.com} 18, 29 -- In-reply-to: {200904071556.n37Fu0dD020190-at-ns.microscopy.com} 18, 29 -- Subject: RE: [Microscopy] Manuals for S-570 SEM 18, 29 -- Date: Tue, 07 Apr 2009 15:39:59 -0400 18, 29 -- Message-id: {007301c9b7b8$a31be6b0$e953b410$-at-ca} 18, 29 -- X-Mailer: Microsoft Office Outlook 12.0 18, 29 -- Content-language: en-us 18, 29 -- Thread-index: Acm3mV3lQBKfOarpQKKSULiW42GaLgAHyDOQ 18, 29 -- Content-Transfer-Encoding: 8bit 18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37Je1rH016051 ==============================End of - Headers==============================
Using ESEM to find bacteria could be difficult if they are embedded in XPS/slime ie you may not recognise them. It might be worth fixing and dehydrating in solvent to remove slime leaving naked bacteria.
Dave
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: 07 April 2009 17:14 To: David Patton
This comes under "Looking for small things in large places".
One of our clients has determined the presence, but not the location, of bacteria in mushroom fruiting bodies. The mushrooms are about the size of the common white mushrooms you buy at the grocery store. He wants to know where the bugs be.
We have tried breaking pieces off the mushroom and viewing them in an environmental SEM and, while we found all sorts of neat reproductive stuff, no bacteria were found. To search systematically through an entire fruiting body with no clue as to where the wee beasties might be can be done, but it's going to be expensive and time-consuming, especially since we don't know their physical appearance.
TEM seems completely impractical for this for obvious reasons (if not, please enlighten me).
So, if anyone has ideas on looking for needles in a fungal haystack, I'm all ears. Real ears, not wood ears.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 9, 27 -- From TindallR-at-missouri.edu Tue Apr 7 11:09:39 2009 9, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G9cl7000497 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:09:39 -0500 9, 27 -- X-IronPort-Anti-Spam-Filtered: true 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEALkX20nRauUo/2dsb2JhbAC/VAEJhwGITYJKgTMG 9, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 9, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 07 Apr 2009 11:09:36 -0500 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 27 -- Tue, 7 Apr 2009 11:09:35 -0500 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="us-ascii" 9, 27 -- Subject: SEM: Locating bacteria in fungi 9, 27 -- Date: Tue, 7 Apr 2009 11:09:35 -0500 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7FC9-at-UM-XMAIL08.um.umsystem.edu} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: SEM: Locating bacteria in fungi 9, 27 -- Thread-Index: Acm3mz5qeov9y91NQHG6WZ82emeIxA== 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 27 -- To: {microscopy-at-microscopy.com} 9, 27 -- X-OriginalArrivalTime: 07 Apr 2009 16:09:35.0394 (UTC) FILETIME=[3E8BE820:01C9B79B] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n37G9cl7000497 ==============================End of - Headers==============================
This incoming email to UWE has been independently scanned for viruses by McAfee anti-virus software and none were detected
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==============================Original Headers============================== 21, 34 -- From David.Patton-at-uwe.ac.uk Wed Apr 8 04:44:42 2009 21, 34 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66]) 21, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n389if8n025417 21, 34 -- for {microscopy-at-microscopy.com} ; Wed, 8 Apr 2009 04:44:41 -0500 21, 34 -- Received: from (unknown [164.11.132.62]) by mailapp04.uwe.ac.uk with smtp 21, 34 -- id 5b24_e132d1ae_2421_11de_8a6c_00142223915c; 21, 34 -- Wed, 08 Apr 2009 10:44:40 +0100 21, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 21, 34 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 21, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 21, 34 -- 2005)) with SMTP id {0KHS00C4Y0EFVF-at-mta02.uwe.ac.uk} for 21, 34 -- microscopy-at-microscopy.com; Wed, 08 Apr 2009 10:44:39 +0100 (BST) 21, 34 -- Date: Wed, 08 Apr 2009 10:42:03 +0100 21, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 21, 34 -- Subject: RE: [Microscopy] SEM: Locating bacteria in fungi 21, 34 -- In-reply-to: {200904071613.n37GDifN009562-at-ns.microscopy.com} 21, 34 -- To: TindallR-at-missouri.edu 21, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDB048C5850-at-egen-uwe01} 21, 34 -- MIME-version: 1.0 21, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 21, 34 -- Content-type: text/plain; charset=us-ascii 21, 34 -- Content-class: urn:content-classes:message 21, 34 -- Thread-topic: [Microscopy] SEM: Locating bacteria in fungi 21, 34 -- Thread-index: Acm3m9+PGFaFp8iwTRGgG8HlVPUakQAkg4Ug 21, 34 -- X-MS-Has-Attach: 21, 34 -- X-MS-TNEF-Correlator: 21, 34 -- References: {200904071613.n37GDifN009562-at-ns.microscopy.com} 21, 34 -- X-NAIMIME-Disclaimer: 1 21, 34 -- X-NAIMIME-Modified: 1 21, 34 -- X-NAI-Spam-Score: 0 21, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 21, 34 -- RV3248=0 21, 34 -- Content-Transfer-Encoding: 8bit 21, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n389if8n025417 ==============================End of - Headers==============================
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Email: dingbiocmu-at-yahoo.com Name: heidi
Organization: Central Mindanao University
Title-Subject: [Filtered] request for protocol for chromosomes aberrations test Using rat bone marrow and rats spermatocytes.
Question: dear listers,
will anyone be kind enough to email me a complete protocols for chromosomes aberrations test using rat bone marrow and rats spermatocytes. Thanks a lot.
When I was performing EM immunostaining in industry, we always assumed that tissue blocks in resin were preserved, but as soon as you section the block, the immediate block face and the sections will undergo changes as a result of being exposed to air and light (where applicable).
In histology, some people will immunostain paraffin embedded slides that are many months old, but our careful time-course comparisons revealed that although immunostaining older slides produced a staining product, the sensitivity was greatly reduced after the first 24-36 hours, and further degradation occurred some 1-2 weeks later. Many antibodies worked fine after a month or two.
I believe that similar results were observed with resin embedding, but I can't remember if I actually read a paper on it, or if we extrapolated the conclusion from histology IHC results.
Someone must have published results on this, don't you think?
Regards, Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu] Sent: Tuesday, April 07, 2009 1:42 PM To: Sobocinski, Gregg
Tissue should be relatively stable in epoxy even at room temperature. Consider Kristina Micheva's (Stephen Smith Lab) Array Tomography technique where LR White sections are repeatedly immunolabeled and each label is eluted with .2M NaOH and .1% SDS before the next label is applied. Of course, there is always an antigen that is the exception. Larry
beth-at-plantbio.uga.edu wrote: } Hi all, How long can slides with sections be stored before they are used for immunolabeling? 6 months in the refrigerator? or longer? or is it a bad idea to wait? I usually cut sections then label the next day but someone here would like to store the slides for awhile if that is an okay practice.
Any advice would be greatly appreciated. thanks, Beth } } PS - I forgot to say that the tissue is embedded in LR White. } } ********************************************************************** } Beth Richardson } Electron Microscopy Lab Coordinator } Plant Biology Department } University of Georgia } Athens, GA 30602-7271 } } http://www.plantbio.uga.edu/emlab/ } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } } "Between the two evils, } I always pick the one I never tried before". Mae West (1893-1980) } ******************************************************************* } } "And it's only the giving that makes you what you are". } Wond'ring Aloud, Jethro Tull (Aqualung) } } *************************************************************************** } The Friends of the Marine Institute - Join Today! } www.friendsofugami.com } } } } } } ==============================Original Headers============================== } 11, 19 -- From beth-at-plantbio.uga.edu Tue Apr 7 11:02:11 2009 } 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) } 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n37G29Kn022252 } 11, 19 -- for {microscopy-at-microscopy.com} ; Tue, 7 Apr 2009 11:02:10 -0500 } 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) } 11, 19 -- (authenticated user beth-at-plantbio.uga.edu) } 11, 19 -- by dogwood.plantbio.uga.edu } 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) } 11, 19 -- for microscopy-at-microscopy.com; } 11, 19 -- Tue, 7 Apr 2009 12:02:05 -0400 } 11, 19 -- Message-Id: {467F2271-FDE6-4830-91B7-95CD62EBC607-at-plantbio.uga.edu} } 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 11, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 11, 19 -- Content-Transfer-Encoding: 7bit } 11, 19 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 11, 19 -- Subject: storing slides - section info } 11, 19 -- Date: Tue, 7 Apr 2009 12:01:40 -0400 } 11, 19 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers============================== }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
I had always assumed that, as you say, the best immuno results were from fresh sections. I further understood that resin embedded sections were fairly impervious to at least some immuno stains (especially of course immuno-gold) and so only the exposed cut surface of the section would present antigens to the label.
If the fewer available antigens in a stored resin section are greatly reduced then I would assume that staining might be more affected than a de-waxed section (or possibly a partially preserved cryo-section).
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: greggps-at-umich.edu
Here is a link to a company that makes motherboards with CPU, PCI and ISA slot(s):
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Title-Subject: [Filtered] Hitachi HF-2000 available
Question: Hi All,
Due to the space limitation, the Hitachi HF-2000 transmission electron microscope installed at Northwestern University (currently in very good working condition) is now available at good price. Please write back you have interests and need more information. It has cold field emission gun (high brightness and energy resolution) and super HRTEM and EDS capability. Many thanks.
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Email: allan.mitchell-at-stonebow.otago.ac.nz Name: Allan Mitchell
Organization: Otago University
Title-Subject: [Filtered] FCS pump speed problem
Question: Hi All
We are having a problem with the liquid nitrogen pump speed on our Leica FCS cryosectioning system (1993 vintage). Does anyone have a copy of the schematics for the FC S controller boards and interconnecting wiring diagram and also the pump control board that they can send as a PDF file, or fax to us.
That would help us a lot to get to the bottom of our problem.
Regards
Allan
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy and Structural Biology Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone 00 64(03) 479 5642 or 479 7301 Fax 0064 (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ NZ Microscopy Society: http://microscopynz.co.nz/
We are currently using a automated stage (x, y and z axis) on our Zeiss Axioplan reflected light scope. Unfortunately the stage is, judging by its groaning, reaching the end of it's product life.
Does anyone know of a manufacturer or company what makes or refurbishes motor driven stages? The plot complication is the stage must be comparable with Clemex drivers and software.
Vendors are welcome and encouraged to respond. I can be reached at frank_karl-at-lincolnelectric.com
Thanks in advance.............. Frank Karl
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==============================Original Headers============================== 7, 21 -- From frank_karl-at-lincolnelectric.com Thu Apr 9 12:42:34 2009 7, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n39HgXTa019061 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 12:42:33 -0500 7, 21 -- Subject: motorized stage replacement needed 7, 21 -- To: Microscopy-at-microscopy.com 7, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 7, 21 -- Message-ID: {OFF749E3EF.6277FB97-ON85257593.00605D89-85257593.0061435E-at-lincolnelectric.com} 7, 21 -- Date: Thu, 9 Apr 2009 13:42:19 -0400 7, 21 -- From: Frank_Karl-at-lincolnelectric.com 7, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 7, 21 -- 07, 2008) at 04/09/2009 01:42:17 PM, 7, 21 -- CD-MIME complete at 04/09/2009 01:42:17 PM, 7, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 7, 21 -- 07, 2008) at 04/09/2009 01:42:17 PM, 7, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 7, 21 -- 07, 2008) at 04/09/2009 01:42:17 PM, 7, 21 -- Serialize complete at 04/09/2009 01:42:17 PM 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
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Question: I was contacted by a researcher who wants to do cross-sectional analysis of fish fin rays, which are somewhat brittle structures. He wants to be able to use light microscopy to image the rings that are present within the fin rays. I assume the rays would have to be embedded, then cut and perhaps polished.
I would like to ask any list members with related experience in this area for advice--how to fix, process, what type of resin, etc. Thank you,
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Email: parmiterd-at-mail.nih.gov Name: David Parmiter
Organization: SAIC - Frederick
Title-Subject: [Filtered] Correction
Question: Hello all -
Sorry about this, but there is a small correction to my previous post:
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Email: parmiterd-at-mail.nih.gov Name: David Parmiter
Organization: SAIC - Frederick
Title-Subject: [Filtered] Correction
Question: Hello all -
Sorry about this, but there is a small correction to my previous post:
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Email: parmiterd-at-mail.nih.gov Name: David Parmiter
Organization: SAIC - Frederick
Title-Subject: [Filtered] Correction
Question: Hello all -
Sorry about this, but there is a small correction to my previous post:
Looking back to LaB6 SEMs, what would you suggest as a viable, reliable and supportable tool? This should include computer control, 5-axis motorized ability, digital capture, turbo vacuum, load lock as an asset (not for wafers and not necessary), 3.1mm pin stub accommodation, easy maintenance, mag to 500KX. Zero power when off. Pin stubs at 12mm diameter up to 25mm diameter. Think of Hitachi Type II stage. There is no need for huge stage size.
Maker or brand is of no significance. Computer control and digital capture are essential. What I do not know is the time frame for transition from Polaroids to digital. Resolution of about 2nm at 20KV is good..lesser is considered. But this is not a hard and fast figure.
If one looks at the old models of the major makers, what are the ones that come to the top? I'd say that ten to twelve year of age are old but could be viable.
And, what do you think these tools would be worth or priced at?
Any thoughts?
Off-line is probably good.
gary g.
==============================Original Headers============================== 9, 17 -- From gary-at-gaugler.com Thu Apr 9 23:04:24 2009 9, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3A44O3S028055 9, 17 -- for {microscopy-at-microscopy.com} ; Thu, 9 Apr 2009 23:04:24 -0500 9, 17 -- Message-Id: {200904100404.n3A44O3S028055-at-ns.microscopy.com} 9, 17 -- Received: (qmail 6860 invoked from network); 9 Apr 2009 21:01:00 -0700 9, 17 -- Received: by simscan 1.1.0 ppid: 6853, pid: 6856, t: 0.1434s 9, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 17 -- by smtp2 with SMTP; 9 Apr 2009 21:01:00 -0700 9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 17 -- Date: Thu, 09 Apr 2009 21:04:10 -0700 9, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 9, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 17 -- Subject: Rhetorical question about a SEM 9, 17 -- Mime-Version: 1.0 9, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
We are a multi user facility using a web event calender for instrument reservations. We are changing this calender system and would like to get suggestions from those of you using a calender system designed for instruments. Any suggestions
Thank you
-- Fred A. Hayes Manager, Central Facilities Department of Chemical Engineering and Material Sciences 3118 Bainer Hall Bainer Hall Drive UC Davis Davis, CA 95616 530-752-0284 office 530-754-6350 fax 707-761-9045 cell fahayes-at-ucdavis.edu http://www.matscicf.ucdavis.edu/
==============================Original Headers============================== 11, 19 -- From fahayes-at-ucdavis.edu Fri Apr 10 13:42:08 2009 11, 19 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3AIg80N013162 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 13:42:08 -0500 11, 19 -- Received: from [169.237.230.148] ([169.237.230.148]) 11, 19 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n3AIg7x3012353 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 11:42:07 -0700 (PDT) 11, 19 -- Message-ID: {49DF92FF.6050607-at-ucdavis.edu} 11, 19 -- Date: Fri, 10 Apr 2009 11:42:07 -0700 11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 11, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 11, 19 -- MIME-Version: 1.0 11, 19 -- To: Microscopy-at-Microscopy.Com 11, 19 -- Subject: suggestions for an instrument electronic signup calender 11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on av8 11, 19 -- X-Virus-Status: Clean 11, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32 ==============================End of - Headers==============================
You didn't say what you are using nor why you are changing, but have a look at our calendar: https://bcrc.bio.umass.edu/cmreserve/day.php?day I'm fairly sure outsiders can view it.
It goes by "MRBS" (MeetingRoomBookingSystem) - http://mrbs.sourceforge.net/
We like it. I don't manage it; the guru (Steve Brewer) at our BCRC set it up. http://www.bio.umass.edu/biology/bcrc/
We used a system based on a wiki before (again, Steve set it up). That was nice in a different way - very flexible and you could leave messages and post notices. It also left an edit record of old versions which was sometimes handy to mediate disputes....
Dale
fahayes-at-ucdavis.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We are a multi user facility using a web event calender for instrument } reservations. We are changing this calender system and would like to get } suggestions from those of you using a calender system designed for } instruments. Any suggestions } } Thank you }
You may have interest in trying our Facility Online Manager (FOM) software for your facility management. FOM has the following features you may appreciate:
. Written in Java and use Microsoft SQL engine. FOM is modularized and easy to expand or customize to meet your facility needs. . Flexible scheduling and fee structure. For each instrument, manager may set different schedule increment, daytime start/end, schedule limitations, and billing policy. Instruments may be charged per use, per actual used time, or combination of reserved and actual used time. Charge calculation is rounded to one minute. . Reservations, cancellations, modifications, early logons, late-comers, extended sessions, conflicting reservations, no-shows, forgot logoffs, auto-logoffs, reserve for users, training sessions, service sessions, repair sessions... All scenarios are covered. . Unlimited discount structures for special user groups. . Consumable sales and inventory. For each instrument, manager may set unlimited consumables associated and charge to user separately. Separate consumable sales also available. Managers receive warning messages when consumable is running out. . Operation manuals online. For each instrument, manager may set unlimited documents for users to download. . Solid access control. FOM uses (optional) hardware relay switches to control access to the instruments. This ensures the user logon FOM when every time the instrument is used. . FOM has been customized for Northwestern University to use of NetID login. It is possible to integrate FOM with any existing online passport system (need inter-server communication). . Optional magnetic card login. . FOM has been customized for Northwestern University to be fully compatible with NUFS financial system. It is possible to customize FOM to generate financial reports following any established accounting system. . FOM provides fairly complicated and flexible rules to validate user financial accounts. For Northwestern University, FOM dynamically validates user NUFS strings against NU financial system. Automatic email warnings will be sent when user account expires . Numerous reports. Customized reports are available upon requests. . Clear administration structure. System admin, facility admin, instrument manager, supervisor, and user. . Easy user communications. Automatic notices, email lists. . Easy to add new facilities. It takes one minute for System Admin to add another entry in the database. Then Facility Admin will be able to login and add new instruments. Existing users have access to the new facility right away without separate registration of accounts. . Easy to add new instruments. Just take a few minutes to configure parameters for the new instruments. . Complete control of usage records. Batch import usage records from existing databases available. . FOM has been licensed to more than ten laboratories at Northwestern University and six other universities. . Please visit http://www.FOMNetworks.com/ to see more details about FOM.
If you want to try the software online, I can send you test login accounts. Please feel free to contact me if you have questions regarding Facility Online Manager software and related facility management issues.
Thank you, Shuyou _____________________________ Shuyou Li, Ph.D. Electron Microscopist and IT Specialist NUANCE Center Northwestern University 2220 Campus Drive, Cook Hall RM#2036 Evanston, IL 60208-3108, USA Ph: (847) 491-6723 Fax: (847) 467-6573 Email: syli-at-northwestern.edu http://www.nuance.northwestern.edu/
-----Original Message----- X-from: fahayes-at-ucdavis.edu [mailto:fahayes-at-ucdavis.edu] Sent: Friday, April 10, 2009 1:47 PM To: syli-at-northwestern.edu
We are a multi user facility using a web event calender for instrument reservations. We are changing this calender system and would like to get suggestions from those of you using a calender system designed for instruments. Any suggestions
Thank you
-- Fred A. Hayes Manager, Central Facilities Department of Chemical Engineering and Material Sciences 3118 Bainer Hall Bainer Hall Drive UC Davis Davis, CA 95616 530-752-0284 office 530-754-6350 fax 707-761-9045 cell fahayes-at-ucdavis.edu http://www.matscicf.ucdavis.edu/
==============================Original Headers============================== 11, 19 -- From fahayes-at-ucdavis.edu Fri Apr 10 13:42:08 2009 11, 19 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3AIg80N013162 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 13:42:08 -0500 11, 19 -- Received: from [169.237.230.148] ([169.237.230.148]) 11, 19 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id n3AIg7x3012353 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 10 Apr 2009 11:42:07 -0700 (PDT) 11, 19 -- Message-ID: {49DF92FF.6050607-at-ucdavis.edu} 11, 19 -- Date: Fri, 10 Apr 2009 11:42:07 -0700 11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 11, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 11, 19 -- MIME-Version: 1.0 11, 19 -- To: Microscopy-at-Microscopy.Com 11, 19 -- Subject: suggestions for an instrument electronic signup calender 11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- X-Virus-Scanned: ClamAV version 0.94.2, clamav-milter version 0.94.2 on av8 11, 19 -- X-Virus-Status: Clean 11, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32 ==============================End of - Headers==============================
From gesiot-at-infinito.it Sun Apr 12 03:39:08 2009 Return-Path: {gesiot-at-infinito.it} Received: from google.com ([95.58.145.119]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3C8d5XY017437 for {microscopylistserverarchive-at-microscopy.com} ; Sun, 12 Apr 2009 03:39:07 -0500 Received: from [123.140.39.80] (HELO google.com) by sharplousywith.cn; Sun, 12 Apr 2009 14:39:00 +0700
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fwang-at-phys.ualberta.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fwang-at-phys.ualberta.ca Name: Feng
Organization: Brookhaven National Lab
Title-Subject: [Filtered] TEM sample preparation by Microtome
Question: Hi all,
We hope to prepare cross-section TEM samples from micron-size powder (like transition metal oxides), and wonder if we can embed this kind of powder into epoxy and cut it into thin slices (such as 20-40nm in thickness) by some special mirotome.
Since I don't have any experience with microtome for TEM sample preparation, any suggestions on possiblities, requirement, tricks, would be appreciated.
Also we may think of buying the facilities if such kind of work can be done with microtome, please let me know if you have any related informaiton, thank you.
I hoping to get input about a certain consensus on the issue of using Miller indices for notation of crystallographic planes and directions in the TEM literature.
The conventions about Miller indices notation in real space are relatively clear. For example planes are denoted by round brackets e.g. (100), directions by square brackets: [100], symmetrically equivalent planes by curly brackets : {100}, and symmetrically equivalent directions by angle brackets: {100} .
The situation becomes confusing when reciprocal space notations are introduced. The most widely accepted convention as far as I am aware is that planes in reciprocal space or reflections are denoted with Miller indices without brackets e.g. 100. Directions are denoted with asterisk as superscript e.g. 100* but also [100]* is used, so which one should be used? If it is [100]* then denoting reflections without brackets is inconsistent, it should be (100)*. Using curly brackets for denoting planes/reflections in reciprocal space is not proper, but how should symmetrically equivalent planes/ reflections in reciprocal space be denoted? Maybe by using asterisk but if brackets are not proper for specific planes/reflections in reciprocal space then why they should be proper for curly brackets? Similar question arises for the notation of symmetrically equivalent directions in reciprocal space.
Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
The Microscopy Society of Northeast Ohio (www.msneo.org) announces their spring 09 meeting to be held at the Cleveland Museum of Art on May 6.
This meeting has been in the making for five years while the museum underwent refurbishing. The museum now houses some of the most modern conservation labs in the area.
The meeting starts at 4:00 with wine and cheese followed by a tour of the labs and dinner. Our speaker will be Dean Yoder, Conservator of Paintings, who will present “Conservation of Apollo and four Muses by the artist Charles Meynier.â€
This is an unprecedented behind-the-scenes look at the Cleveland Museum of Art and will be of interest to the microscopist, chemist and art lovers. Comments and observations of the meeting are welcome at the MSNO blog at http://www.msneo.org/newsletters.html.
RSVP to Pat Glazebrook no later than May 1, 2009. Pat can be reached at pglazebrook-at-metrohealth.org or (216) 778-8958.
PAY ONLINE THROUGH PAYPAL AT: http://www.msneo.org/meetings.html
stay safe......... Frank -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 23 -- From frank_karl-at-lincolnelectric.com Mon Apr 13 08:52:40 2009 11, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DDqeSX018039 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 08:52:40 -0500 11, 23 -- Subject: Microscopy Society of Northeast Ohio spring meeting 11, 23 -- To: Microscopy-at-microscopy.com 11, 23 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 23 -- Message-ID: {OFF82F3AF9.521C923C-ON85257597.004C08B4-85257597.004C3247-at-lincolnelectric.com} 11, 23 -- Date: Mon, 13 Apr 2009 09:52:22 -0400 11, 23 -- From: Frank_Karl-at-lincolnelectric.com 11, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 11, 23 -- 07, 2008) at 04/13/2009 09:52:24 AM, 11, 23 -- CD-MIME complete at 04/13/2009 09:52:24 AM, 11, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 11, 23 -- 07, 2008) at 04/13/2009 09:52:24 AM, 11, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 11, 23 -- 07, 2008) at 04/13/2009 09:52:24 AM, 11, 23 -- Serialize complete at 04/13/2009 09:52:24 AM 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- charset="UTF-8" 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id n3DDqeSX018039 ==============================End of - Headers==============================
We routinely embed powders in epoxy and cut them normally. Usually it's as simple as just putting a pinch of dry powder in the tip of a microcentrifuge tube and filling it with resin. Make sure the powder is really dry. Don't use a lot of powder----just a smidgen.
We generally cut sections that are 70-85 nm, but I don't see any reason you can't go thinner. 20 might be stretching it, especially since some particles can pop out of the sections, even in thicker slices.
Be very careful with your knife. If you're using diamond knives, test the powder on an old, beat up one in case the particles nick the edge. Or use glass.
Hope this helps.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: fwang-at-phys.ualberta.ca [mailto:fwang-at-phys.ualberta.ca] Sent: Sunday, April 12, 2009 8:54 AM To: Tindall, Randy D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both fwang-at-phys.ualberta.ca as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: fwang-at-phys.ualberta.ca Name: Feng
Organization: Brookhaven National Lab
Title-Subject: [Filtered] TEM sample preparation by Microtome
Question: Hi all,
We hope to prepare cross-section TEM samples from micron-size powder (like transition metal oxides), and wonder if we can embed this kind of powder into epoxy and cut it into thin slices (such as 20-40nm in thickness) by some special mirotome.
Since I don't have any experience with microtome for TEM sample preparation, any suggestions on possiblities, requirement, tricks, would be appreciated.
Also we may think of buying the facilities if such kind of work can be done with microtome, please let me know if you have any related informaiton, thank you.
==============================Original Headers============================== 12, 11 -- From zaluzec-at-microscopy.com Sun Apr 12 08:52:57 2009 12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3CDqt7s012053 12, 11 -- for {microscopy-at-microscopy.com} ; Sun, 12 Apr 2009 08:52:57 -0500 12, 11 -- Mime-Version: 1.0 12, 11 -- Message-Id: {p06240800c607a2a94a36-at-[206.69.208.22]} 12, 11 -- Date: Sun, 12 Apr 2009 08:52:55 -0500 12, 11 -- To: microscopy-at-microscopy.com 12, 11 -- From: fwang-at-phys.ualberta.ca (by way of MicroscopyListserver) 12, 11 -- Subject: viaWWW: TEM sample preparation by Microtome 12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 26, 30 -- From TindallR-at-missouri.edu Mon Apr 13 09:02:30 2009 26, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DE2T5R032163 26, 30 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 09:02:30 -0500 26, 30 -- X-IronPort-Anti-Spam-Filtered: true 26, 30 -- X-IronPort-Anti-Spam-Result: ApoEAIrh4knRauUo/2dsb2JhbAC+FQEBAQeETohNgkkBAYExBg 26, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 26, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 13 Apr 2009 09:02:29 -0500 26, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 26, 30 -- Mon, 13 Apr 2009 09:02:29 -0500 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 30 -- Content-class: urn:content-classes:message 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Type: text/plain; 26, 30 -- charset="us-ascii" 26, 30 -- Subject: RE: [Microscopy] viaWWW: TEM sample preparation by Microtome 26, 30 -- Date: Mon, 13 Apr 2009 09:01:29 -0500 26, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD802D-at-UM-XMAIL08.um.umsystem.edu} 26, 30 -- In-Reply-To: {200904121354.n3CDsS59013745-at-ns.microscopy.com} 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- Thread-Topic: [Microscopy] viaWWW: TEM sample preparation by Microtome 26, 30 -- Thread-Index: Acm7djMpkBarGKbsTdu0BKkt3RoFlgAyWQvQ 26, 30 -- References: {200904121354.n3CDsS59013745-at-ns.microscopy.com} 26, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 26, 30 -- To: {fwang-at-phys.ualberta.ca} 26, 30 -- Cc: {microscopy-at-microscopy.com} 26, 30 -- X-OriginalArrivalTime: 13 Apr 2009 14:02:29.0088 (UTC) FILETIME=[7B647600:01C9BC40] 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3DE2T5R032163 ==============================End of - Headers==============================
From nbkjgdn-at-iahhgyaon.tpts4.seed.nz Mon Apr 13 10:12:03 2009 Return-Path: {nbkjgdn-at-iahhgyaon.tpts4.seed.nz} Received: from google.com (133.sub-70-194-178.myvzw.com [70.194.178.133]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DFC2gp016303 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 13 Apr 2009 10:12:02 -0500 Received: from [153.90.98.144] (HELO google.com) by cozyice.cn; Mon, 13 Apr 2009 11:12:02 -0400 Message-ID: {00000006CB38698766543101} Reply-To: Roly Wiggins {16394spraginslorelle-at-gmail.com}
Dear All:
I would like to have your comments on the performance of Leica UC6rt ultramicortome. This is the model dedicated to room temperature sectioning. Please reply to me off-line. Thank you all very much in advance.
Hong
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==============================Original Headers============================== 7, 32 -- From hyi-at-emory.edu Mon Apr 13 13:09:15 2009 7, 32 -- Received: from mr1.cc.emory.edu (mr1.cc.emory.edu [170.140.52.90]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DI9FmJ023693 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 13:09:15 -0500 7, 32 -- Received: from EXCHEDGE1.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 32 -- by mr1.cc.emory.edu (8.13.1/8.13.1) with ESMTP id n3DI8uth030951 7, 32 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 14:08:56 -0400 7, 32 -- Received: from EXCHHUB2.Enterprise.emory.net (170.140.30.54) by 7, 32 -- EXCHEDGE1.enterprise.emory.net (170.140.52.33) with Microsoft SMTP Server 7, 32 -- (TLS) id 8.1.358.0; Mon, 13 Apr 2009 14:08:52 -0400 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 7, 32 -- EXCHHUB2.Enterprise.emory.net ([170.140.30.54]) with mapi; Mon, 13 Apr 2009 7, 32 -- 14:08:55 -0400 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} 7, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 32 -- Date: Mon, 13 Apr 2009 14:08:54 -0400 7, 32 -- Subject: UC6rt 7, 32 -- Thread-Topic: UC6rt 7, 32 -- Thread-Index: Acm8YufioZrv+3a5A0O5WcYrgzVQBw== 7, 32 -- Message-ID: {C608F7F6.AC00%hyi-at-emory.edu} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- acceptlanguage: en-US 7, 32 -- Content-Type: text/plain; charset="iso-8859-1" 7, 32 -- MIME-Version: 1.0 7, 32 -- X-emory.edu-MailScanner: Found to be clean 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu 7, 32 -- X-Spam-Status: No 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3DI9FmJ023693 ==============================End of - Headers==============================
The M&M 2009 program is set, sessions are scheduled, and the count down to the Richmond meeting is on.
I encourage all students, or those of you who have students, attending the meeting to please consider the bursary program sponsored by the MSA education committee. The purpose of this program is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where they can meet and interact with the established microscopy community while defraying some meeting costs.
The students work for approximately 20 hours (or up to 40 hours) during the meeting and pre-meeting events and are paid $10 an hour. The jobs involve such things as providing support in the different symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth or volunteer office, monitoring use of the Internet Café, and helping with poster set-up and take-down.
Each participant will be sent the task list where they can sign-up for the jobs and times they want. In most instances students end up *working* sessions they would attend anyway. There is an added bonus of a $10 cash meal allotment for each morning and/or afternoon session worked.
For those *non-students* we could always use volunteers to help fill-in with the above mentioned meeting activities as well. Although not paid on an hourly basis as the student bursaries, volunteers do receive some compensation along with the same cash allotment for meals. They also have the added benefit of getting to interact more with the microscopy community as they assist with meeting tasks.
Those interested in the bursary program should check the *I wish to apply for a student bursary* box in section 2 of the registration form (must be members of MSA or MAS, and enrolled as students at a recognized educational institution).
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me (off-line).
Amanda Lawrence Electron Microscope Center Mississippi State University 662-325-3019 alawrence-at-entomology.msstate.edu
==============================Original Headers============================== 12, 20 -- From ALawrence-at-entomology.msstate.edu Mon Apr 13 16:47:16 2009 12, 20 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3DLlGEN012316 12, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 16:47:16 -0500 12, 20 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197]) 12, 20 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n3DLlFZu009186 12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 12, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 16:47:15 -0500 12, 20 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu 12, 20 -- with Novell_GroupWise; Mon, 13 Apr 2009 16:47:15 -0500 12, 20 -- Message-Id: {49E36C8D.B26A.00E6.0-at-entomology.msstate.edu} 12, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 12, 20 -- Date: Mon, 13 Apr 2009 16:47:09 -0500 12, 20 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu} 12, 20 -- To: {microscopy-at-microscopy.com} 12, 20 -- Subject: M&M 2009 student bursaries and volunteers 12, 20 -- Mime-Version: 1.0 12, 20 -- Content-Type: text/plain; charset=ISO-8859-15 12, 20 -- Content-Transfer-Encoding: 8bit 12, 20 -- Content-Disposition: inline ==============================End of - Headers==============================
I'm getting ready to "teach" my undergraduate EM class how to create a poster presentation of their work and I need a little educating as well. Could you please send me your sage advice on creating a poster in PowerPoint (that's what we are required to use) regarding the resolution of the electron micrographs? As background, we are developing TEM film, scanning the negatives, and manipulating them with Adobe Photoshop.
Thanks, Kristen
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology Frostburg State University
HI Kristen There is lots to recommend Power point but posters isn't its strongest suite. Our media specialist tells me the Power Point's output size is one of its weak points Poster size width is determined by the printer being used (our is 43 inches) while the maximum length is 56 inches Lengths greater than 56 leave the printer scratching its head though there are work arounds.
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com] Sent: Monday, April 13, 2009 6:19 PM To: rjharris-at-uwo.ca
Hi All,
I'm getting ready to "teach" my undergraduate EM class how to create a poster presentation of their work and I need a little educating as well. Could you please send me your sage advice on creating a poster in PowerPoint (that's what we are required to use) regarding the resolution of the electron micrographs? As background, we are developing TEM film, scanning the negatives, and manipulating them with Adobe Photoshop.
Thanks, Kristen
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology Frostburg State University
Malcolm, Now that I think about it, one of the trickiest variables I've observed when immunolabeling is the high degree of variability of antigen robustness. Some antibodies show consistent immunostaining patterns for years on sectioned paraffin slides, and are not sensitive to fixation variables. Others, especially recent proliferation protein antibodies and some other "high tech" antibodies, show high immunostaining variability depending on: 1) the fixative type, 2) the timing of fixation (sensitivity to over and underfixation), and 3) the tissue section age (as queried in this original topic). Yet another variable is the introduction and refinement of new antigen retrieval/unmasking techniques over the past fifteen years or so.
Yes, the antibodies do not penetrate beyond the cut surface of the resin, unless the resin is reduced with sodium ethaoxide. I agree with you that conserving the few antigen binding sites exposed on the EM section should be first priority, which is why I assume most people use fresh sections.
I haven't been able to find any publications to share at this time. My apologies for the delayed response, as I mistakenly thought I'd have time to perform a literature search.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: Malcolm Haswell [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Wednesday, April 08, 2009 12:16 PM To: Sobocinski, Gregg; Microscopy MSA
We would like to invite all to attend the Southeastern Microscopy Society (SEMS) 09 meeting to be held in Athens, GA May 27-29. SEMS is a great venue for networking with others in microscopy/ microanalysis related fields, developing new collaborations, learning about state-of-the-art tools for microscopy/microanalysis from vendors, and catching up with friends (www.southeasternmicrosocpy.org) .
Invited speakers for this year*s meeting are: Dr. Sara Miller, Duke University; Dr. Jay Jerome, Vanderbilt University; Dr. Wilma Lingle, Mayo Clinic, Rochester, MN; and Dr. Yiping Zhao, University of Georgia. Short courses include: RMC Cryo Sectioning, EMS QuantomiX, Leica Live Cell Confocal Multi Photon Imaging, Hitachi Tabletop SEM TM-1000 Demo, and a PhotoShop Workshop hosted by Dr. Jay Jerome. There will also be numerous platform and poster presentations as well as vendor talks and demos.
We encourage all to register for the SEMS meeting and perhaps consider contributing to the meeting by way of platform or poster presentation. You can go to http://southeasternmicroscopy.org/index-2.htmlto register and find forms for abstract submission (call for papers; deadline has been extended to April 17), technician travel award, Ruska Award, and photo contest entry. A preliminary program can be found in the brochure online. An undated program will be posted to this site soon. You can also go to http://www.georgiacenter.uga.edu/conferences/2009/May/27/sms.phtml to register for SEMS directly.
Please pass the word to collaborators, faculty, staff and students and join us at SEMS 09. I look forward to seeing you in Athens next month.
cheers, Giselle
SEMS President
Giselle Thibaudeau, Director Electron Microscope Center Associate Professor of Biological Sciences Mississippi State University phone 662-325-3017 email giselle-at-emcenter.msstate.edu
==============================Original Headers============================== 9, 20 -- From ALawrence-at-entomology.msstate.edu Tue Apr 14 12:52:46 2009 9, 20 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EHqjC9008529 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 12:52:46 -0500 9, 20 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197]) 9, 20 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id n3EHqj1Q015870 9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 12:52:45 -0500 9, 20 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu 9, 20 -- with Novell_GroupWise; Tue, 14 Apr 2009 12:52:45 -0500 9, 20 -- Message-Id: {49E4871B.B26A.00E6.0-at-entomology.msstate.edu} 9, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 9, 20 -- Date: Tue, 14 Apr 2009 12:52:44 -0500 9, 20 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu} 9, 20 -- To: {microscopy-at-microscopy.com} 9, 20 -- Subject: Southeastern Microscopy annual meeting 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=ISO-8859-15 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- Content-Disposition: inline ==============================End of - Headers==============================
-----Original Message----- X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca] Sent: Tuesday, April 14, 2009 9:11 AM To: dkloos-at-parallaxray.com
HI Kristen There is lots to recommend Power point but posters isn't its strongest suite. Our media specialist tells me the Power Point's output size is one of its weak points Poster size width is determined by the printer being used (our is 43 inches) while the maximum length is 56 inches Lengths greater than 56 leave the printer scratching its head though there are work arounds.
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com] Sent: Monday, April 13, 2009 6:19 PM To: rjharris-at-uwo.ca
Hi All,
I'm getting ready to "teach" my undergraduate EM class how to create a poster presentation of their work and I need a little educating as well. Could you please send me your sage advice on creating a poster in PowerPoint (that's what we are required to use) regarding the resolution of the electron micrographs? As background, we are developing TEM film, scanning the negatives, and manipulating them with Adobe Photoshop.
Thanks, Kristen
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology Frostburg State University
We need to disassemble and pack a used Jeol JEM 200CX TEM in San Francisco area (No reassembling service needed at this time) so that we can load it to a truck for moving. To reduce our cost, our company will provide you with an assistant if two people's work is needed during the disassembling process. The working time is flexible and you may work during weekend.
If you are interested, please submit your bid with your information to lisa-at-glosuntech.com. Contract will be given to the one with the lowest bid price.
Thank you!
Regards,
Lisa
Glosuntech LLC
==============================Original Headers============================== 7, 31 -- From lisa-at-glosuntech.com Tue Apr 14 15:04:54 2009 7, 31 -- Received: from mta4.brinkster.com (mta4.brinkster.com [65.182.109.73]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EK4scC008778 7, 31 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Apr 2009 15:04:54 -0500 7, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id 6DE3C5EDE0B 7, 31 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Apr 2009 16:04:51 -0400 (EDT) 7, 31 -- X-Virus-Scanned: amavisd-new at 7, 31 -- X-Spam-Flag: NO 7, 31 -- X-Spam-Score: -2.499 7, 31 -- X-Spam-Level: 7, 31 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=5 7, 31 -- tests=[BAYES_00=-2.599, RDNS_NONE=0.1] 7, 31 -- Received: from mta4.brinkster.com ([127.0.0.1]) 7, 31 -- by localhost (mta4.brinkster.com [127.0.0.1]) (amavisd-new, port 10024) 7, 31 -- with ESMTP id UItkXdwH4OFH for {Microscopy-at-Microscopy.Com} ; 7, 31 -- Tue, 14 Apr 2009 16:04:46 -0400 (EDT) 7, 31 -- Received: from mail10a.brinkster.com (mail10a.brinkster.com [10.0.6.217]) 7, 31 -- by mta4.brinkster.com (Postfix) with ESMTP id DDFBC5EDE75 7, 31 -- for {Microscopy-at-Microscopy.Com} ; Tue, 14 Apr 2009 16:04:45 -0400 (EDT) 7, 31 -- Date: Tue, 14 Apr 2009 16:04:47 -0400 (EDT) 7, 31 -- From: lisa-at-glosuntech.com 7, 31 -- To: Microscopy-at-Microscopy.Com 7, 31 -- Message-ID: {29065733.31591239739487398.JavaMail.root-at-mail10a.brinkster.com} 7, 31 -- In-Reply-To: {31101946.31551239739412083.JavaMail.root-at-mail10a.brinkster.com} 7, 31 -- Subject: TEM Disassemble service needed 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; charset=utf-8 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- X-Originating-IP: [75.140.108.220] 7, 31 -- X-Mailer: Zimbra 5.0.8_GA_2463.RHEL4 (ZimbraWebClient - FF3.0 (Win)/5.0.8_GA_2462.RHEL4_64) ==============================End of - Headers==============================
You and your students will be fine with PowerPoint. For posters larger than what PowerPoint's setup allows, we scale a little down, keeping the desired proportion, then we increase the size (e.g., 123%) during printing.
I have been using PP for posters since 2000, with different printers. Until 2-3 years ago, we would have to go with our files to a centralized printing facility, but now we have our own large HP printer, and even new users have little problem using it from their desktops. I've been meaning to switch to Adobe InDesign for posters for years, but guess what, whenever the time comes to make a new poster, there is always a hurry, and never enough time to learn new software! I am neither lover or hater of Microsoft products, use them as a tool, and PPoint has been very convenient for us.
I recommend making all adjustments to your photos while still in Photoshop, and all labels, lettering, and scale in PowerPoint. This way it'll be easier to do late adjustments like font size. We switch PS color management off and try to achieve the desired gray levels on all images. Once everything is to our liking, we reduce the pixel size and save under different name.
Now, to your particular question - we aim for 150-200 pixels per inch in the resulting full-size poster print. That's from some early testing we did. We set picture size in PS, in inches, to what it approximately is going to be in the final print and then set resolution to 150-200 ppi. Then save the new image separately as TIFF, that's the best and should not cause any file size issue these days. Then we insert (or drag-n-drop) those saved images onto the poster in PPoint. It is very common to have to resize the image a bit this or that way before the poster is finished, that's why the ppi range. 175 ppi is a good starting number. You'll get a feeling after a few posters. Don't be afraid to explore lower ppi - as long as you see all relevant features, of course, images printed from lower ppi tend to look better than oversampled (too high ppi) images on a poster. Below 150 ppi in the final print, you may notice the reduced resolution, but it won't look bad. As long as you use TIFFs, PowerPoint will do nice smoothening, and there'll be no pixelation.
Good luck. Please don't hesitate to contact me directly if I wasn't clear, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
Begin forwarded message:
} From: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com} } Date: April 13, 2009 6:14:52 PM EDT } To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com} } Subject: [Microscopy] Advice: Resolution of micrographs for posters? } Reply-To: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi All, } } I'm getting ready to "teach" my undergraduate EM class how to create } a poster presentation of their work and I need a little educating as } well. Could you please send me your sage advice on creating a poster } in PowerPoint (that's what we are required to use) regarding the } resolution of the electron micrographs? As background, we are } developing TEM film, scanning the negatives, and manipulating them } with Adobe Photoshop. } } Thanks, } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } Frostburg State University } } kalennon-at-frostburg.edu } } } } } ==============================Original } Headers============================== } 8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009 } 8, 20 -- Received: from web84001.mail.mud.yahoo.com } (web84001.mail.mud.yahoo.com [68.142.206.171]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id n3DME1fS026918 } 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01 } -0500 } 8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009 } 22:14:01 -0000 } 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=yahoo.com; s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr } +0Nro1sSEP2MofzkAUoG+LFo=; h=Message-ID:X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QdDLdklkKjl/ } vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/ } 5pfJcQHaRTqzv0F9Du06x80gL9WfK9tfgY5blF/ } Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99ILd8f0frdvPOc7pH66pXM } = } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- h=Message-ID:X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:MIME-Version:Content-Type; } 8, 20 -- b=CRJXqaYio8A65tWLDZzLUty0rsYt37M } + } 1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2mHCuOFFiQl7wcdQylo85G5dOudaeps122CChWR4mvwBpvW0zUNQt } +9ZB+ajEXgx7b+Mpe2yAzlxL11rki3h2FKA/pqZL+/La5Y=; } 8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com} } 8, 20 -- X-YMail-OSG: } GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3YCQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbxci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQtDwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA-- } 8, 20 -- Received: from [96.239.150.112] by } web84001.mail.mud.yahoo.com via HTTP; Mon, 13 Apr 2009 15:14:00 PDT } 8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/ } 0.7.289.1 } 8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT) } 8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } 8, 20 -- Subject: Advice: Resolution of micrographs for posters? } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 23 -- From vladislav_speransky-at-nih.gov Tue Apr 14 15:38:28 2009 10, 23 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EKcR9X023807 10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 15:38:27 -0500 10, 23 -- X-IronPortListener: NIH_Relay 10, 23 -- X-SBRS: None 10, 23 -- X-IronPort-AV: E=Sophos;i="4.40,186,1238990400"; 10, 23 -- d="scan'208";a="83849612" 10, 23 -- Received: from helix.nih.gov ([128.231.2.3]) 10, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 14 Apr 2009 16:38:27 -0400 10, 23 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185]) 10, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id n3EKcRYj014498 10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 16:38:27 -0400 10, 23 -- Message-Id: {FD8A426D-3157-4DE4-8CB3-74D5112051DD-at-nih.gov} 10, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 10, 23 -- Content-Transfer-Encoding: 7bit 10, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 10, 23 -- Subject: Fwd: [Microscopy] Advice: Resolution of micrographs for posters? 10, 23 -- Date: Tue, 14 Apr 2009 16:38:27 -0400 10, 23 -- References: {D13763A4740B07428566B25D7E768DA69F1C1A49-at-NIHMLBX02.nih.gov} 10, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lanqiang76-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: lanqiang76-at-yahoo.com Name: Shawn Lan
Organization: Huntsman
Title-Subject: [Filtered] Manual for Reichert-Jung FC4E microtome
Question: We lost our manual for Reichert FC4E cryo-microtome associated with Reichert-Jung Ultracut E. I called Leica and they do not have the manual any longer becasue the instrument is too old. I am wondering if anybody can help with a copy of the instruction manual. We would like to pay any related copy and mail expenses. Really appreciate the help. Thanks.
Have a customer who, during an interdepartmental move, managed to lose the schematics for their Leo 435VP. Can anyone provide a copy or scan? I'd be happy to reimburse for any expense. Any info on hardware / software upgrades would also be welcome (haven't contacted manufacturer yet, but will). It is currently running Windows 3.1 and has a motherboard with 2 ISA slots that aren't being used and a couple of PCI slots that are. Not sure if there have been any upgrades from the original configuration.
*** Note new phone numbers, email & web addresses Allen R. Sampson, Owner Advanced Research Systems 317 North 4th. Street Saint Charles, Illinois 60174 phone (800) 506-9770 fax (800) 506-9771 email ars-at-advressys.com web www.advressys.com
==============================Original Headers============================== 5, 25 -- From ars-at-advressys.com Tue Apr 14 23:32:48 2009 5, 25 -- Received: from omr9.networksolutionsemail.com (omr9.networksolutionsemail.com [205.178.146.59]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3F4WliT008610 5, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 23:32:47 -0500 5, 25 -- Received: from mail.networksolutionsemail.com (ns-omr9.mgt.netsol.com [10.49.6.72]) 5, 25 -- by omr9.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id n3F4Wl5e016342 5, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 00:32:47 -0400 5, 25 -- Received: (qmail 30622 invoked by uid 78); 15 Apr 2009 04:32:47 -0000 5, 25 -- Received: from unknown (HELO vaiod) (ars-at-advressys.com-at-67.162.79.36) 5, 25 -- by ns-omr9.lb.hosting.dc2.netsol.com with SMTP; 15 Apr 2009 04:32:47 -0000 5, 25 -- Reply-To: {ars-at-advressys.com} 5, 25 -- From: "Allen R. Sampson" {ars-at-advressys.com} 5, 25 -- To: "'Microscopy Listserver'" {Microscopy-at-microscopy.com} 5, 25 -- Subject: schematics for Leo 435 VP 5, 25 -- Date: Tue, 14 Apr 2009 23:32:50 -0500 5, 25 -- Organization: Advanced Research Systems 5, 25 -- Message-ID: {77565B653BEF4654AC57291948FD9EB8-at-vaiod} 5, 25 -- MIME-Version: 1.0 5, 25 -- Content-Type: text/plain; 5, 25 -- charset="iso-8859-1" 5, 25 -- X-Mailer: Microsoft Office Outlook 11 5, 25 -- Thread-Index: Acm9gzuypxIpUXL1Tt+Ppna8WxBsgQ== 5, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3F4WliT008610 ==============================End of - Headers==============================
We're planning to study ZP1, ZP2 and ZP3 protein immunohistochemistry on mice ovary. We couldn't find commercially available antibodies for these proteins suitable for IHC. Does anybody know sources for buying this items? Thanks in advance...
Dr. Necat Yilmaz MD, PhD University of Mersin
==============================Original Headers============================== 4, 31 -- From nyilmaz-at-mersin.edu.tr Wed Apr 15 04:03:57 2009 4, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3F93tbj030441 4, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 04:03:56 -0500 4, 31 -- Received: from localhost (localhost [127.0.0.1]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id DAEA72F710A 4, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 12:03:14 +0300 (EEST) 4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 4, 31 -- by localhost (mail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 4, 31 -- with ESMTP id U5v-lDVb1+ru for {Microscopy-at-microscopy.com} ; 4, 31 -- Wed, 15 Apr 2009 12:02:26 +0300 (EEST) 4, 31 -- Received: from nejat1 (unknown [193.255.129.131]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 2C6EE2F7110 4, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 12:00:02 +0300 (EEST) 4, 31 -- Message-ID: {000b01c9bda8$a6899680$2101a8c0-at-nejat1} 4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 4, 31 -- Subject: Antibody for ZP Proteins 4, 31 -- Date: Wed, 15 Apr 2009 12:00:40 +0300 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; 4, 31 -- format=flowed; 4, 31 -- charset="iso-8859-9"; 4, 31 -- reply-type=response 4, 31 -- Content-Transfer-Encoding: 7bit 4, 31 -- X-Priority: 3 4, 31 -- X-MSMail-Priority: Normal 4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
Generally I use PowerPoint to produce my posters, which is fine as my predecessor created our Core poster in PowerPoint and we never go above poster size A0 [paper size 3 feet x 4 feet]. I have converted a few to MS Publisher though. Other applications can be used but it should be noted that Word will not accept paper size as big as A0. Our University printers do the printing, we just provide the Excel ppt or Publisher pub file.
Image size is kind of irrelevant provided your image resolution is at least 600 pixels per inch on the paper [it can be higher res in the document]. You can often get away with less if that’s all you have [upscaling in Photoshop can help], as often it's only the University/Centre logos that look really naff and pixellated at low-res. Generally it's useful to make all the images quite high resolution, say 1000 pixels per inch, as I quite often nick images from our poster, say for the website, and it's a pain having to track down the original.
You do need Photoshop or similar [Elements or Serif PhotoPlus] to edit/crop/enhance images. Large Powerpoint sizes [Mb file size] due to high resolution graphics isn't a problem for a modern PC [well for mine anyway as it is an imaging workstation and gaming powerhouse], plus it simply goes off to the printers on a CD or RAM drive. Don't use 6,400 dpi scanned TEM ones though unless you are a patient sort on the PC, downscale to or scanning at 1,200 dpi seems fine for most applications [unless of course you are printing that single TEM film image at size A0]. Going below 300 dpi on the printed page might look bad, but it depends a lot on the image itself. For pdf's use Adobes Acrobat Pro's pdf optimiser and generally output for the latest acrobat version compatibility. Our OUCS printers seem to prefer output from ubiquitous PowerPoint or Publisher [word is limited to 22 inches size I believe].
The advantage of Powerpoint is that everyone has it, and it works well at least up to the standard A0 poster size. Microsoft Publisher is probably a better option but it's less common and not MS Office's greatest app [Serif's PagePlus is better and a third of the price]. Plus there's Adobe's Indesign and Quark express for those with deep pockets [probably not students].
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: rjharris-at-uwo.ca [mailto:rjharris-at-uwo.ca] Sent: 14 April 2009 17:09 To: kjmorris-at-well.ox.ac.uk
HI Kristen There is lots to recommend Power point but posters isn't its strongest suite. Our media specialist tells me the Power Point's output size is one of its weak points Poster size width is determined by the printer being used (our is 43 inches) while the maximum length is 56 inches Lengths greater than 56 leave the printer scratching its head though there are work arounds.
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com] Sent: Monday, April 13, 2009 6:19 PM To: rjharris-at-uwo.ca
Hi All,
I'm getting ready to "teach" my undergraduate EM class how to create a poster presentation of their work and I need a little educating as well. Could you please send me your sage advice on creating a poster in PowerPoint (that's what we are required to use) regarding the resolution of the electron micrographs? As background, we are developing TEM film, scanning the negatives, and manipulating them with Adobe Photoshop.
Thanks, Kristen
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology Frostburg State University
Sorry didn't see your posting - all good advice. As we also produce our posters for pdf [where the viewer can zoom] we aim for higher dpi in the original ppt file [doesn't do any harm] - plus I do like to nick them for other uses. I guess many of our images are generally lower than 600 dpi on the actual page, typically 300 dpi, and as you say they still look fine. Nothing wrong with higher resolution though.
Once scaled down on-screen PowerPoint doesn't seem to mind our 'hi-res' images though [at 600 dpi], in terms of PC response when moving around the presentation.
With any image editing/graphics design more PC memory helps a lot [my laptop, 2.25GHz Pentium M] was sluggish with Photoshop CS2 at 512 and 1Gb RAM, but when upgraded to 2Gb [motherboard max] it noticeably improved. Windows 32-bit has the 3-4Gb OS max though [including graphics card memory], and only 64-bit OS goes beyond the 4Gb max [motherboard dependent].
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: 14 April 2009 21:43 To: kjmorris-at-well.ox.ac.uk
Hi Kirsten,
You and your students will be fine with PowerPoint. For posters larger than what PowerPoint's setup allows, we scale a little down, keeping the desired proportion, then we increase the size (e.g., 123%) during printing.
I have been using PP for posters since 2000, with different printers. Until 2-3 years ago, we would have to go with our files to a centralized printing facility, but now we have our own large HP printer, and even new users have little problem using it from their desktops. I've been meaning to switch to Adobe InDesign for posters for years, but guess what, whenever the time comes to make a new poster, there is always a hurry, and never enough time to learn new software! I am neither lover or hater of Microsoft products, use them as a tool, and PPoint has been very convenient for us.
I recommend making all adjustments to your photos while still in Photoshop, and all labels, lettering, and scale in PowerPoint. This way it'll be easier to do late adjustments like font size. We switch PS color management off and try to achieve the desired gray levels on all images. Once everything is to our liking, we reduce the pixel size and save under different name.
Now, to your particular question - we aim for 150-200 pixels per inch in the resulting full-size poster print. That's from some early testing we did. We set picture size in PS, in inches, to what it approximately is going to be in the final print and then set resolution to 150-200 ppi. Then save the new image separately as TIFF, that's the best and should not cause any file size issue these days. Then we insert (or drag-n-drop) those saved images onto the poster in PPoint. It is very common to have to resize the image a bit this or that way before the poster is finished, that's why the ppi range. 175 ppi is a good starting number. You'll get a feeling after a few posters. Don't be afraid to explore lower ppi - as long as you see all relevant features, of course, images printed from lower ppi tend to look better than oversampled (too high ppi) images on a poster. Below 150 ppi in the final print, you may notice the reduced resolution, but it won't look bad. As long as you use TIFFs, PowerPoint will do nice smoothening, and there'll be no pixelation.
Good luck. Please don't hesitate to contact me directly if I wasn't clear, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
Begin forwarded message:
} From: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com} } Date: April 13, 2009 6:14:52 PM EDT } To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com} } Subject: [Microscopy] Advice: Resolution of micrographs for posters? } Reply-To: "kamlennon-at-yahoo.com" {kamlennon-at-yahoo.com} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi All, } } I'm getting ready to "teach" my undergraduate EM class how to create } a poster presentation of their work and I need a little educating as } well. Could you please send me your sage advice on creating a poster } in PowerPoint (that's what we are required to use) regarding the } resolution of the electron micrographs? As background, we are } developing TEM film, scanning the negatives, and manipulating them } with Adobe Photoshop. } } Thanks, } Kristen } } Kristen A. Lennon, Ph.D. } Lecturer, Department of Biology } Frostburg State University } } kalennon-at-frostburg.edu } } } } } ==============================Original } Headers============================== } 8, 20 -- From kamlennon-at-yahoo.com Mon Apr 13 17:14:01 2009 } 8, 20 -- Received: from web84001.mail.mud.yahoo.com } (web84001.mail.mud.yahoo.com [68.142.206.171]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id n3DME1fS026918 } 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 13 Apr 2009 17:14:01 } -0500 } 8, 20 -- Received: (qmail 9792 invoked by uid 60001); 13 Apr 2009 } 22:14:01 -0000 } 8, 20 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=yahoo.com; s=s1024; t=1239660840; bh=Hr4c8e07wXoHDmP5Vlr } +0Nro1sSEP2MofzkAUoG+LFo=; h=Message-ID:X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=QdDLdklkKjl/ } vE/h2oGJ25pp3xV1w3EbUjqpV15TLg1Zr6A/ } 5pfJcQHaRTqzv0F9Du06x80gL9WfK9tfgY5blF/ } Fty4CVRapPqIEuVqzVYZJdNdp0hsTsZgsbUNOXqQrE47M8Q3WRil6P7zxv9Mv99ILd8f0frdvPOc 7pH66pXM } = } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- h=Message-ID:X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:MIME-Version:Content-Type; } 8, 20 -- b=CRJXqaYio8A65tWLDZzLUty0rsYt37M } + } 1zmFke8nyQmsSxP5eSw3IP902oyf57dVuhsdE69j2mHCuOFFiQl7wcdQylo85G5dOudaeps122CC hWR4mvwBpvW0zUNQt } +9ZB+ajEXgx7b+Mpe2yAzlxL11rki3h2FKA/pqZL+/La5Y=; } 8, 20 -- Message-ID: {900517.8328.qm-at-web84001.mail.mud.yahoo.com} } 8, 20 -- X-YMail-OSG: } GOaI4dkVM1lf.MdnEMrDzh7VguZZKyiqckCPLo6AQI0OazVY.J7mb8d48LdabpA7uxHRhCirAh.4 WblMTVBt6xrw7cHe9mAD0PeQIGj3gfPMF72CPTgkBoJqdCFjbmOM35O6eOHtngfec2j2sPU.vV3Y CQY4YQ40TsBIjfdJMizoMuaF99mujg61TMpl138WjdAXH_UvfWXx4SRRZJ2GRw6Kks1l4MQ2VXbx ci1ya6BX2jmGZvT8Nb9tX_MrK.xzc0QppZVu58ExAF_qPjMzhHW_8uEnSeU6LLzFcha2HwyW_LQt DwCZrJu4NwmLQtsBhQXM111TYqYk5_rdEhYxlDvDsA-- } 8, 20 -- Received: from [96.239.150.112] by } web84001.mail.mud.yahoo.com via HTTP; Mon, 13 Apr 2009 15:14:00 PDT } 8, 20 -- X-Mailer: YahooMailClassic/5.2.15 YahooMailWebService/ } 0.7.289.1 } 8, 20 -- Date: Mon, 13 Apr 2009 15:14:00 -0700 (PDT) } 8, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} } 8, 20 -- Subject: Advice: Resolution of micrographs for posters? } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=us-ascii } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 23 -- From vladislav_speransky-at-nih.gov Tue Apr 14 15:38:28 2009 10, 23 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3EKcR9X023807 10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 15:38:27 -0500 10, 23 -- X-IronPortListener: NIH_Relay 10, 23 -- X-SBRS: None 10, 23 -- X-IronPort-AV: E=Sophos;i="4.40,186,1238990400"; 10, 23 -- d="scan'208";a="83849612" 10, 23 -- Received: from helix.nih.gov ([128.231.2.3]) 10, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 14 Apr 2009 16:38:27 -0400 10, 23 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185]) 10, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id n3EKcRYj014498 10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 14 Apr 2009 16:38:27 -0400 10, 23 -- Message-Id: {FD8A426D-3157-4DE4-8CB3-74D5112051DD-at-nih.gov} 10, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 10, 23 -- Content-Transfer-Encoding: 7bit 10, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 10, 23 -- Subject: Fwd: [Microscopy] Advice: Resolution of micrographs for posters? 10, 23 -- Date: Tue, 14 Apr 2009 16:38:27 -0400 10, 23 -- References: {D13763A4740B07428566B25D7E768DA69F1C1A49-at-NIHMLBX02.nih.gov} 10, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 22, 22 -- From kjmorris-at-well.ox.ac.uk Wed Apr 15 06:26:19 2009 22, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3FBQG4e031166 22, 22 -- for {microscopy-at-microscopy.com} ; Wed, 15 Apr 2009 06:26:17 -0500 22, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 22, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 22, 22 -- id 1Lu3Fv-0007OP-ST 22, 22 -- for microscopy-at-microscopy.com; Wed, 15 Apr 2009 12:26:16 +0100 22, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 22, 22 -- To: {microscopy-at-microscopy.com} 22, 22 -- References: {200904142043.n3EKh0Ir001615-at-ns.microscopy.com} 22, 22 -- Subject: RE: [Microscopy] Fwd: Advice: Resolution of micrographs for posters? 22, 22 -- Date: Wed, 15 Apr 2009 12:26:15 +0100 22, 22 -- Message-ID: {331636B55C43479E95C18D815613E408-at-CytoWhizz} 22, 22 -- MIME-Version: 1.0 22, 22 -- Content-Type: text/plain; 22, 22 -- charset="us-ascii" 22, 22 -- Content-Transfer-Encoding: 7bit 22, 22 -- X-Mailer: Microsoft Office Outlook 11 22, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 22, 22 -- Thread-Index: Acm9QZqJk9tmskdvQoCnBp5RqqBW4gAeS60g 22, 22 -- In-Reply-To: {200904142043.n3EKh0Ir001615-at-ns.microscopy.com} ==============================End of - Headers==============================
What you are saying comes from the definition of reciprocal and direct space and it is perfectly logical. The problem is as you point out, that it is confusing and also it is not always used in the literature in consistent fashion.
The confusing points arise usually for non-cubic and especially for non-orthogonal crystal systems.
Consider a monoclinic crystal with beta the angle of monoclinicity. A diffraction pattern which consists of 100 and 010 reflections is defined as [001] zone axis, that is the el. beam is parallel to the normal of the plane [001] in reciprocal space and that is what we call the beam direction denoted usually B=[001]. The [001] direction in real space and the vector B, the normal to the [001] plane in reciprocal space are parallel, but (001) plane in direct space is not necessarily parallel to the [001] plane in reciprocal space.
So when one writes [001] it is quite ambiguous whether we refer to plane or direction until one specifies that it refers to either direct or reciprocal space. All this defies the purpose of notation. If we need to use narrative clarification or the meaning of the context to make a notation clear then that notation is useless. In trying to avoid such confusing point in the literature are accepted different approaches e.g. the use of asterisk to denote directions e.g. 001* would define a vector normal to the (001) plane to avoid the need to write (001) and specify that it is not the plane in real space that is referred to but actually the normal to it.
So if we use some consistent notation for example denoting reflections in reciprocal space as [001]* which will be read as "the normal to the (001) planes in real space with magnitude 1/d001" and (001)* - the plane in reciprocal space normal to the [001] direction in real space. Then no context clarification or explicit explanation would be necessary.
Krassimir. ================================ Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
} The notation is definitely not standard, but I simply invert real } and reciprocal space. } } Real direction [uvw], {uvw} } Real plane (hkl), {hkl} } } Reciprocal direction (hkl), {hkl} } Reciprocal plane [uvw], {uvw} } } I do this because (i) planes in direct space are closely related to } directions in reciprocal space, and (ii) it maintains the elegant } symmetry/duality. } } It does cause some cognitive dissonance when the parameter being } described (i.e. momentum) is clearly a vector, but defined in } reciprocal space. Then, the natural tendency to denote a vector in } "hard", square brackets is confronted by the factor that the vector } is in the wrong space. } } I can deal with the cognitive dissonance. } } Lew Rabenberg } } On Apr 12, 2009, at 6:21 PM, bozhilov-at-ucr.edu wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } I hoping to get input about a certain consensus on the issue of } } using } } Miller indices for notation of crystallographic planes and } } directions } } in the TEM literature. } } } } The conventions about Miller indices notation in real space are } } relatively clear. For example planes are denoted by round brackets } } e.g. (100), directions by square brackets: [100], symmetrically } } equivalent planes by curly brackets : {100}, and symmetrically } } equivalent directions by angle brackets: {100} . } } } } The situation becomes confusing when reciprocal space notations are } } introduced. } } The most widely accepted convention as far as I am aware is that } } planes in reciprocal space or reflections are denoted with Miller } } indices without brackets e.g. 100. } } Directions are denoted with asterisk as superscript e.g. 100* but } } also [100]* is used, so which one should be used? If it is [100]* } } then denoting reflections without brackets is inconsistent, it should } } be (100)*. } } Using curly brackets for denoting planes/reflections in reciprocal } } space is not proper, but how should symmetrically equivalent planes/ } } reflections in reciprocal space be denoted? Maybe by using asterisk } } but if brackets are not proper for specific planes/reflections in } } reciprocal space then why they should be proper for curly brackets? } } Similar question arises for the notation of symmetrically equivalent } } directions in reciprocal space. } } } } Krassimir N. Bozhilov } } Central Facility for Advanced Microscopy and Microanalysis } } University of California } } Riverside, CA 92521 } } } } tel. 951 827 2998 } } fax 951 827 2489 } } bozhilov-at-ucr.edu } } } } } } } } } } ==============================Original } } Headers============================== } } 9, 22 -- From bozhilov-at-ucr.edu Sun Apr 12 18:19:46 2009 } } 9, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu } } [138.23.226.212]) } } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n3CNJjRf026159 } } 9, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2009 } } 18:19:46 -0500 } } 9, 22 -- Received: from [192.168.100.104] } } (vpn-138-23-234-83.ucr.edu [138.23.234.83]) } } 9, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA) } } 9, 22 -- with ESMTP id DPT77235 (AUTH bozhilov-at-ucr.edu) } } 9, 22 -- for {Microscopy-at-microscopy.com} ; } } 9, 22 -- Sun, 12 Apr 2009 16:19:45 -0700 (PDT) } } 9, 22 -- Message-Id: {87820979-F803-463D-AF2E-F6F989C56C59-at-ucr.edu} } } 9, 22 -- From: KN Bozhilov {bozhilov-at-ucr.edu} } } 9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } 9, 22 -- In-Reply-To: {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8} } } 9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } } delsp=yes } } 9, 22 -- Content-Transfer-Encoding: 7bit } } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) } } 9, 22 -- Subject: Miller indices } } 9, 22 -- X-Priority: 3 } } 9, 22 -- Date: Sun, 12 Apr 2009 16:19:44 -0700 } } 9, 22 -- References: } } {200805082342.m48NgkeG022691-at-ns.microscopy.com} } } {012701c8b269$5aa28650$b99fcb96-at-siap6segs20pa8} {457BCD4F-3FD9-4599-8363-2AF1A5775159-at-ucr.edu } } } {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8} } } 9, 22 -- X-Mailer: Apple Mail (2.930.3) } } 9, 22 -- X-Junkmail-Status: score=0/50, host= } } ==============================End of - } } Headers============================== }
Enough confusion appears to be added in the discussion. I think simply the following notations are usually agreed, especially in physical sciences:
1. In real space:
(hkl) one plane {hkl} family of equivalent planes And also, it is usually necessary to differentiate (hkl) from (-h-k-l)
[uvw] one direction {uvw} family of equivalent directions [uvw] and [-u-v-w] are usually NOT differentiated from each other
2. In reciprocal space:
Vector g* or any r* expressed as g*(r*) = ha* + kb* + lc*
In diffraction pattern: The end point of a reciprocal vector (reciprocal lattice node), or a diffraction spot, or a diffraction ring, is labeled as hkl NO BRACKETS AT ALL
3. Zone of a single crystal pattern is expressed as [uvw] which is (more or less) the electron beam direction in REAL SPACE. Square brackets are necessary!
Also, context is important. Hope this clarifies.
Chaoying Ni, PhD W.M. Keck Electron Microscopy Facility Facility location: 022 Spencer Laboratory Mailing address: 201 duPont Hall Dept. of Materials Sci. and Eng. University of Delaware Newark, DE 19716 (302) 831-2318 (Phone) (302) 831-4545 (Fax) http://eml.masc.udel.edu
-----Original Message----- X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] Sent: Wednesday, April 15, 2009 2:49 PM To: cni-at-UDel.Edu
Lew,
What you are saying comes from the definition of reciprocal and direct space and it is perfectly logical. The problem is as you point out, that it is confusing and also it is not always used in the literature in consistent fashion.
The confusing points arise usually for non-cubic and especially for non-orthogonal crystal systems.
Consider a monoclinic crystal with beta the angle of monoclinicity. A diffraction pattern which consists of 100 and 010 reflections is defined as [001] zone axis, that is the el. beam is parallel to the normal of the plane [001] in reciprocal space and that is what we call the beam direction denoted usually B=[001]. The [001] direction in real space and the vector B, the normal to the [001] plane in reciprocal space are parallel, but (001) plane in direct space is not necessarily parallel to the [001] plane in reciprocal space.
So when one writes [001] it is quite ambiguous whether we refer to plane or direction until one specifies that it refers to either direct or reciprocal space. All this defies the purpose of notation. If we need to use narrative clarification or the meaning of the context to make a notation clear then that notation is useless. In trying to avoid such confusing point in the literature are accepted different approaches e.g. the use of asterisk to denote directions e.g. 001* would define a vector normal to the (001) plane to avoid the need to write (001) and specify that it is not the plane in real space that is referred to but actually the normal to it.
So if we use some consistent notation for example denoting reflections in reciprocal space as [001]* which will be read as "the normal to the (001) planes in real space with magnitude 1/d001" and (001)* - the plane in reciprocal space normal to the [001] direction in real space. Then no context clarification or explicit explanation would be necessary.
Krassimir. ================================ Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
} The notation is definitely not standard, but I simply invert real } and reciprocal space. } } Real direction [uvw], {uvw} } Real plane (hkl), {hkl} } } Reciprocal direction (hkl), {hkl} } Reciprocal plane [uvw], {uvw} } } I do this because (i) planes in direct space are closely related to } directions in reciprocal space, and (ii) it maintains the elegant } symmetry/duality. } } It does cause some cognitive dissonance when the parameter being } described (i.e. momentum) is clearly a vector, but defined in } reciprocal space. Then, the natural tendency to denote a vector in } "hard", square brackets is confronted by the factor that the vector } is in the wrong space. } } I can deal with the cognitive dissonance. } } Lew Rabenberg } } On Apr 12, 2009, at 6:21 PM, bozhilov-at-ucr.edu wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } I hoping to get input about a certain consensus on the issue of } } using } } Miller indices for notation of crystallographic planes and } } directions } } in the TEM literature. } } } } The conventions about Miller indices notation in real space are } } relatively clear. For example planes are denoted by round brackets } } e.g. (100), directions by square brackets: [100], symmetrically } } equivalent planes by curly brackets : {100}, and symmetrically } } equivalent directions by angle brackets: {100} . } } } } The situation becomes confusing when reciprocal space notations are } } introduced. } } The most widely accepted convention as far as I am aware is that } } planes in reciprocal space or reflections are denoted with Miller } } indices without brackets e.g. 100. } } Directions are denoted with asterisk as superscript e.g. 100* but } } also [100]* is used, so which one should be used? If it is [100]* } } then denoting reflections without brackets is inconsistent, it should } } be (100)*. } } Using curly brackets for denoting planes/reflections in reciprocal } } space is not proper, but how should symmetrically equivalent planes/ } } reflections in reciprocal space be denoted? Maybe by using asterisk } } but if brackets are not proper for specific planes/reflections in } } reciprocal space then why they should be proper for curly brackets? } } Similar question arises for the notation of symmetrically equivalent } } directions in reciprocal space. } } } } Krassimir N. Bozhilov } } Central Facility for Advanced Microscopy and Microanalysis } } University of California } } Riverside, CA 92521 } } } } tel. 951 827 2998 } } fax 951 827 2489 } } bozhilov-at-ucr.edu } } } } } } } } } } ==============================Original } } Headers============================== } } 9, 22 -- From bozhilov-at-ucr.edu Sun Apr 12 18:19:46 2009 } } 9, 22 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu } } [138.23.226.212]) } } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n3CNJjRf026159 } } 9, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Apr 2009 } } 18:19:46 -0500 } } 9, 22 -- Received: from [192.168.100.104] } } (vpn-138-23-234-83.ucr.edu [138.23.234.83]) } } 9, 22 -- by sentrell.ucr.edu (MOS 3.10.5-GA) } } 9, 22 -- with ESMTP id DPT77235 (AUTH bozhilov-at-ucr.edu) } } 9, 22 -- for {Microscopy-at-microscopy.com} ; } } 9, 22 -- Sun, 12 Apr 2009 16:19:45 -0700 (PDT) } } 9, 22 -- Message-Id: {87820979-F803-463D-AF2E-F6F989C56C59-at-ucr.edu} } } 9, 22 -- From: KN Bozhilov {bozhilov-at-ucr.edu} } } 9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } 9, 22 -- In-Reply-To: {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8} } } 9, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } } delsp=yes } } 9, 22 -- Content-Transfer-Encoding: 7bit } } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) } } 9, 22 -- Subject: Miller indices } } 9, 22 -- X-Priority: 3 } } 9, 22 -- Date: Sun, 12 Apr 2009 16:19:44 -0700 } } 9, 22 -- References: } } {200805082342.m48NgkeG022691-at-ns.microscopy.com} } } {012701c8b269$5aa28650$b99fcb96-at-siap6segs20pa8} {457BCD4F-3FD9-4599-8363-2AF1A5775159-at-ucr.edu } } } {006a01c8b30a$009a3490$b99fcb96-at-siap6segs20pa8} } } 9, 22 -- X-Mailer: Apple Mail (2.930.3) } } 9, 22 -- X-Junkmail-Status: score=0/50, host= } } ==============================End of - } } Headers============================== }
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Title-Subject: [Filtered] Hitachi S-800 SEM available
Question: We stopped using our Hitachi S-800 SEM two years ago when we purchased a tabletop model and, coincidentally, the video went out in our S-800. The unit has been sitting idle since then and it's time for it to go.
This microscope is available to any organization willing to come in, disassemble it, and arrange for shipping. Preference will be given to universities.
Our school is using PowerPoint as default software for creating posters for many years.
While 300 ppi is a "golden standard" for printers (glossy magazines), it is surely overkill for posters printed with inkjet printers. From my experience printing images from XL30 SEM (about 1400*1000 pixels) at 11" image size (i.e. 127 ppi) produce good results. Even 100 ppi on poster could be good enough. But for line drawings you need higher resolution.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
It looks like we a pushed (gently, for now) to consider possibility of switching our service contracts to insurance - based contracts. So, now I am very interested in opinions on this type of servicing microscopes - SEM and TEM.
Thank you,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Push back----and don't be gentle. We tried this a number of years ago and it was a disaster. It once took us nine months to schedule preventive maintenance, since an insurance provider wasn't paying its bills and the service provider understandably went on strike.
Despite what they told us when they were selling us these contracts, the procedure to get service was MUCH more complicated and time-consuming and we were given a much lower priority by the vendors than their service contract holders were. You may have to justify service and parts before they will authorize it.
Maybe we just had a poor experience and things are better now, but the day we went back on vendor service contracts for our scopes was one of the happiest days of my life.
Have you considered third-party service providers contracts? Some people say they are very happy with these. I have no personal experience with them, however.
Good luck, Randy
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Thursday, April 16, 2009 11:23 AM To: Tindall, Randy D.
Hi everybody,
It looks like we a pushed (gently, for now) to consider possibility of switching our service contracts to insurance - based contracts. So, now I am very interested in opinions on this type of servicing microscopes - SEM and TEM.
Thank you,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
I used one in a previous incarnation (job elsewhere). It was fine, saved money, got the microscope company service. Then we had a big claim and a diagnostic procedure -- looking for stray magnetic fields. Suddenly the contract was worthless. The insurance company refused to pay the claim (contrary to their saleman's statements) because of the magnetic field survey. When I explained why the survey and what it was, they (supposedly) referred the claim to their engineers, who may be great on elevators and escalators, but know nothing of EMs, who denied the claim. They had paid the microscope company, but they were trying to get me to re-imburse them. I referred them to the University lawyers (said University having several contracts with this company for various equipment). I don't know how it finally ended, but I didn't give the insurance company any money, and as soon as the contract was up, went to the microscope company for a contract.
Where I am now, we have company contracts and no hint of insurance contracts, and I'd fight them pump and electron to avoid changing.
Phil
} Hi everybody, } } It looks like we a pushed (gently, for now) to consider possibility of } switching our service contracts to insurance - based contracts. So, now } I am very interested in opinions on this type of servicing microscopes - } SEM and TEM. } } Thank you, } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Vladimir- I've always had contracts, but my colleague at a nearby institution was forced to go with insurance. They have an older TEM and at one point needed a major repair that took over 2 weeks....charged hourly. The end result was a bill close to if not more than the vendor's contract would have been. They also have trouble getting their PM visits scheduled, since labs under contract do get priority. Lee } } } Hi everybody, } } } } It looks like we a pushed (gently, for now) to consider possibility of } } switching our service contracts to insurance - based contracts. So, now } } I am very interested in opinions on this type of servicing microscopes - } } SEM and TEM. } } } } Thank you, } } } } Vladimir } } } } Vladimir M. Dusevich, Ph.D. } } Electron Microscope Lab Manager } } 371 School of Dentistry } } 650 E. 25th Street } } Kansas City, MO 64108-2784 } } } } Phone: (816) 235-2072 } } Fax: (816) 235-5524 } } Web: http://www.umkc.edu/dentistry/microscopy } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } ==============================Original Headers============================== } 5, 26 -- From oshel1pe-at-cmich.edu Thu Apr 16 12:31:56 2009 } 5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n3GHVu5f029068 } 5, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Apr 2009 } 12:31:56 -0500 } 5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP } id n3GHVsnD000652; } 5, 26 -- Thu, 16 Apr 2009 13:31:54 -0400 } 5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by } egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); } 5, 26 -- Thu, 16 Apr 2009 13:31:50 -0400 } 5, 26 -- Mime-Version: 1.0 } 5, 26 -- Message-Id: {f06240802c60d1a08643c-at-[141.209.160.249]} } 5, 26 -- In-Reply-To: {200904161625.n3GGPga9005060-at-ns.microscopy.com} } 5, 26 -- References: {200904161625.n3GGPga9005060-at-ns.microscopy.com} } 5, 26 -- Date: Thu, 16 Apr 2009 13:31:48 -0400 } 5, 26 -- To: DusevichV-at-umkc.edu } 5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 5, 26 -- Subject: Re: [Microscopy] insurance - based contracts } 5, 26 -- Cc: Microscopy-at-microscopy.com } 5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 5, 26 -- X-OriginalArrivalTime: 16 Apr 2009 17:31:50.0748 (UTC) } FILETIME=[39F6E9C0:01C9BEB9] } 5, 26 -- X-Canit-CHI2: 0.00 } 5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 5, 26 -- X-Spam-Score: -4.20 () [Hold at 5.00] } L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) } 5, 26 -- X-CanItPRO-Stream: default } 5, 26 -- X-Canit-Stats-ID: 11939391 - da1cb7a8bc4d } 5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } ==============================End of - Headers==============================
-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
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Email: donald.gibbon-at-matcoinc.com Name: Donald L. Gibbon
Organization: MATCO Services Inc
Title-Subject: [Filtered] TEM powder electrron diffraction analysis
Question: In the olden days before SEM/EDS made it appear that just anyone could identify almost anything in the materials world, microscopists would use the diffraction capabilities of transmission electron microscopes to distinguish amorphous and crystalline substances and determine the d-spacings of the crystalline parts. Powder patterns were routinely created using vapor-cast gold, platinum or aluminum as internal standards and then analyzed by measuring the diameters of the rings, converting the linear measurements into d-spacings using a handy slide rule. I am sure many of you do not believe this era is still within living memory. But I assure you it is.
Today, only having access to SEM/EDS at my work place, I am constantly frustrated by not being able to do what I used to do routinely 40 years ago. For example, I want to determine the crystalline species forming a thin layer on a copper coupon. Again, in the olden days I would have used my evaporator to carbon-coat a glass slide, hold it briefly in the fumes of HF, float the carbon off on water, pick up a piece of the thin carbon film on a 3mm grid, crudely scrape the coupon over the grid with a spatula to deposit a powder on the carbon film, coat it with a thin layer of Al metal, put it in the TEM and in about five minutes have a powder pattern. Develop and print the pattern, measure it and have the list of d-spacings in just a few more minutes. Many common species could be identified from the patterns by inspection.
Today I could use Auger or EDS to identify the elemental components of the film but I can't nail the structure/compound. If there's enough material I might be able to determine the index of refraction with oils (does any one else remember how to do that?)... but powder electron diffraction is SO easy and so definitive!
To REALLY blow your minds, consider how few people have capabilities to do reflection electron diffraction right off the surface of the copper coupon, with the deposit actually in place. THAT is the simplest technique in the world: just orient the sample surface nearly vertical(parallel to the beam) and advance it into the beam. You'll be amazed to see a diffraction pattern appear on the screen of the microscope, just like Davison and Germer did when they determined the wave nature of the electron in the 1920s. What a buzz!
With all that build up, is there anyone out there offering this TEM diffraction service? I'd particularly like to know someone near Pittsburgh, so I could be with them when they do the analysis. If you have the equipment and don't know the technique, I'll show you how to do it!
I serviced TEM/SEM tools first working for Philips, then as independent, servicing and moving all brands plus associated instruments and equipment. You may get lucky for awhile and save some money, until major problem happens. Such as defective HT tank for example. Insurance will try to locate a used one, or an independent service provider that will repair it. I did that. But insurance is unlikely to pay tens of thousands of $$ when all else fails.
There is no miracle. This kind of insurance does not have enough base to be a reliable one, even if they try hard- this will be mathematically impossible in the end. It is difficult enough for an OEM to make service and support business profitable while dealing directly with end users. How can you add a middleman in-between and expect profit? Please don't do this.
If life comes to the point when your employer can't afford an OEM or a 3rd party contract - do you own PMs and pay directly to service providers when their help needed. And keep your fingers crossed that nothing major will go. It sounds scary, but works better than insurance based service. Common sense versus peace of mind.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {DusevichV-at-umkc.edu} To: {vitalylazar-at-att.net} Sent: Thursday, April 16, 2009 12:23 PM
I'm looking for a very large chambered environmental SEM with EDS capabilities to examine a friable firebrick that is roughly 6 inches cubed. Thank you for any leads.
Joe
==============================Original Headers============================== 3, 20 -- From Joe.Kintz-at-stress.com Fri Apr 17 07:39:38 2009 3, 20 -- Received: from mxgate.stress.com (mxgate.stress.com [12.96.4.229]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3HCdao0027652 3, 20 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 07:39:37 -0500 3, 20 -- Received: from jkintz ([172.16.12.133]) by mail.stress.com with Microsoft SMTPSVC(6.0.3790.1830); Fri, 17 Apr 2009 07:39:35 -0500 3, 20 -- From: "Joe Kintz" {joe.kintz-at-stress.com} 3, 20 -- To: {microscopy-at-microscopy.com} 3, 20 -- References: {200904170810.n3H8AeUW017439-at-ns.microscopy.com} 3, 20 -- Subject: Need very large chamber environmental SEM with EDS 3, 20 -- Date: Fri, 17 Apr 2009 07:39:35 -0500 3, 20 -- Message-ID: {00d601c9bf59$904e3990$850c10ac-at-stress.com} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain;charset="us-ascii" 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Mailer: Microsoft Office Outlook 11 3, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 3, 20 -- In-Reply-To: {200904170810.n3H8AeUW017439-at-ns.microscopy.com} 3, 20 -- Thread-Index: Acm/NARp9bon5zBDSzKJhqJf8azqcQAJSwhw 3, 20 -- X-OriginalArrivalTime: 17 Apr 2009 12:39:35.0465 (UTC) FILETIME=[9088D190:01C9BF59] 3, 20 -- X-SCA-Stop: [cust-96647FDDB5F74CA0B2173CACB4D94086,18,39,0] ==============================End of - Headers==============================
} On Fri, Apr 17, 2009 at 6:53 AM, {joe.kintz-at-stress.com} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } I'm looking for a very large chambered environmental SEM with EDS } } capabilities to examine a friable firebrick that is roughly 6 inches cubed. } } Thank you for any leads. } } } } Joe } } } }
==============================Original Headers============================== 8, 36 -- From ian.drucker-at-gmail.com Fri Apr 17 10:45:35 2009 8, 36 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.246]) 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3HFjZFq030514 8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 10:45:35 -0500 8, 36 -- Received: by rv-out-0708.google.com with SMTP id l33so936557rvb.30 8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Apr 2009 08:45:35 -0700 (PDT) 8, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 36 -- d=gmail.com; s=gamma; 8, 36 -- h=domainkey-signature:mime-version:received:in-reply-to:references 8, 36 -- :from:date:message-id:subject:to:content-type 8, 36 -- :content-transfer-encoding; 8, 36 -- bh=w4/XykWgXigqxx0C7yzK2sQ6VZPpGirSVhI9PpCtCbA=; 8, 36 -- b=BIM1lsBKAyg5iy9T8gxXgP/fe6h9YfCZmaKDcvGhJJUsAIe7cSfG6N1YttlRQoftpH 8, 36 -- W7fiSnFmM1GCHr1C+sxHWmwKZ3dDS03uYQm+EmdzOukritZ6pXUZcA+TrN9OUI7FPpvO 8, 36 -- IYYwg1u3/UzGelKePxE785U3ialitqK7TSetU= 8, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 8, 36 -- d=gmail.com; s=gamma; 8, 36 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 8, 36 -- :content-type:content-transfer-encoding; 8, 36 -- b=w6rmnkP47rYv40Y8bk6RZg18DalsYbytTVeU6pSbHrH4Uv3HObwmv7wTbDKc5LJjyh 8, 36 -- lshg9j9NJvFCWkXAoQVWkfgwQstFT2RMaSOtadF26E7noZ5qHYbAnqAA8mMki/z4wO3z 8, 36 -- pMHRGvGro6Z7HOtAvDIjKwEq2sAw3cHAY4XDU= 8, 36 -- MIME-Version: 1.0 8, 36 -- Received: by 10.141.115.20 with SMTP id s20mr1251060rvm.285.1239983135072; 8, 36 -- Fri, 17 Apr 2009 08:45:35 -0700 (PDT) 8, 36 -- In-Reply-To: {c0bfda950904170842s1f651230w874d8d367d997508-at-mail.gmail.com} 8, 36 -- References: {200904171253.n3HCreNn011050-at-ns.microscopy.com} 8, 36 -- {c0bfda950904170842s1f651230w874d8d367d997508-at-mail.gmail.com} 8, 36 -- From: ID {ian.drucker-at-gmail.com} 8, 36 -- Date: Fri, 17 Apr 2009 09:45:20 -0600 8, 36 -- Message-ID: {c0bfda950904170845o37d71e4bn2ec979e23f72814b-at-mail.gmail.com} 8, 36 -- Subject: Re: [Microscopy] Need very large chamber environmental SEM with EDS 8, 36 -- To: Microscopy-at-microscopy.com 8, 36 -- Content-Type: text/plain; charset=ISO-8859-1 8, 36 -- Content-Transfer-Encoding: 8bit 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3HFjZFq030514 ==============================End of - Headers==============================
I have inherited a Biowave DFR-10 (a fancy Ted Pella microwave) but I did not inherit the owner's manual.
Does anyone have a copy that they can send me?
Thanks and have a nice weekend.
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
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Email: gas19-at-chrysler.com Name: Gerald Shulke
Organization: Chrysler LLC
Title-Subject: [Filtered] Re: [Microscopy] Need very large chamber environmental SEM with EDS
Question: I think that you will find most of the major manufacturers will have an ESEM that can accommodate large samples: Hitachi, JEOL, Tescan, FEI, and Zeiss. X and Y stage travel will most likely not be able to accommodate the total limits of your sample, but you will be able to reposition your sample on the stage to get across your sample. I think your limiting factor will be the height of the sample, and being able to do EDS. 6 inches is pretty tall. I think you can rule out several manufacturers based on this alone. Most ESEMs have a small WD for EDS, which will work to your advantage. If you cannot cut your sample and must have 6 inches of height, then you are very limited. I don't have any experience with the scopes that might accommodate that height. There seems to be a couple that may, but you need to ask the right questions to them to see if EDS on a 6 inch cube is truly possible. If you are able to cut the sample, say in half, then you've opened the door for just about all of the manufacturers. Now you can compare all of the features of the ESEMs, not just the height limitation.
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Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: Central States Microscopy and Microanalysis Society
Title-Subject: [Filtered] Spring Meeting
Question: Central States Microscopy and Microanalysis Society wish to invite you to their spring meeting in Columbia Mo. The theme is Scince is Art. For more information or to register please contact Clarissa Wisner, cvierret-at-mst.edu.
Program includes a wide variety of speakers:
Kristy Wendt, Wash U Research Engineer - National Nanotechnology Infrastructure Network
Dr. Paul Mainwaring, Gatan Inc. - X-ray Microscopy and Tomography of Low Z Materials in the SEM
Dr. Adrienne Hoard, Professor of Design and Color Theory from MU - Color Theory in Imaging
Dr. Jay Jerome Department of Pathology, Vanderbilt University Medical Center - Putting the Art in Microscopy Images While Keeping the Science
Dr. Ye Duan, Assistant Professor of Computer Science at MU 3D Computer Imaging in Science and Medicine
Dr. Gary Greenberg Author of A Grain of Sand: Natureís Secret Wonder - The Art of Science: From Pythagoras to the Present
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Email: lolitageorge-at-gmail.com Name: Lolita
Title-Subject: [Filtered] Total RNA extraction from glutaraldehyde fixed tissues...
Question: Can we efficiently extract total RNA from samples fixed using sod.cacodylate buffer and Glutaraldehyde? I would like to do some Real time PCR analysis and need some total RNA from the same sections where I plan to do EM studies.
I am researching the use of time-gated luminescence microscopy to minimise autofluorescence.
The research team I work with makes its own lanthanide organometallic fluorophores Eu3+ and Tb3+ which last about 3ms. But I am now getting specimens from collaborators who sometimes want to label the specimens at their end. Unfortunately lanthanides are not easy to use and require UV excitation. So I am trying to find some easy-to-use commercial long lifetime fluorophores. A lifetime of 1000ns would be great. I have had some excellent suggestions so far such as:
1. Organometallic transition metal-ligand complexes such as ruthenium (Ru II), rhenium (Re I), or osmium (Os II) with one or more diimine ligands (10 ns to 10 ìs). 2. Organic fluorophores such as Pyrene (400ns) or Coronene (200ns). 3. Quantum dots. 4. Triplet probes such as eosin or erythrosin.
But I am having trouble sourcing the correct probes and I am not sure how easy they are to use. I would be grateful if you could tell me about any commercial easy-to-use around lifetime fluorophores you have used.
Regards,
-- Tom Lawson tomxlawson-at-gmail.com PhD Student Dept. of Physics Macquarie University NSW, 2109 Australia
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The Midwest Microscopy and Microanalysis Society will hold its third annual Optical Techniques Workshop on Thursday, May 28, 2009. This year's program on basics of polarized light microscopy will include lectures, applications demonstrations and hands-on training. Details and registration information can be found on our website under Meetings:
www.midwestmicroscopy.org
Space for the hands-on portion of the program will be limited, so please RSVP early if you plan to attend. We look forward to seeing you there.
Regards,
Elaine Schumacher M3S Program Chair
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com {mailto:eschumacher-at-mccrone.com} Web Site: www.mccrone.com {http://www.mccrone.com/}
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 12, 23 -- From eschumacher-at-mccrone.com Mon Apr 20 09:24:39 2009 12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3KEOcmZ014568 12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Apr 2009 09:24:38 -0500 12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 12, 23 -- Mon, 20 Apr 2009 09:23:24 -0500 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="US-ASCII" 12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society Optical Workshop 12, 23 -- Date: Mon, 20 Apr 2009 09:24:25 -0500 12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A7021320CD-at-MCCRONEMSG.tmg.mccrone.com} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society Optical Workshop 12, 23 -- Thread-Index: AcnBw7TQXcP5ZctZR2ywQUD/d+l1dw== 12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 23 -- To: {microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 20 Apr 2009 14:23:24.0528 (UTC) FILETIME=[9095A700:01C9C1C3] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3KEOcmZ014568 ==============================End of - Headers==============================
In my continuing quest to find out how to finish putting the bulb assembly back together on my fluorescence microscope I've found out some additional information.
The power supply and bulb assembly were made by a company called "OptiQuip". I have called the company, which still exists, but have yet to receive a return phone call.
Anyway, if you have such a beasty and have put the bulb assembly back together, it holds either a xenon or mercury bulb, please let me know.
Even OptiQuip's owner's manual does not have a drawing or explain it clearly enough for me to understand.
Once more with feeling,
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 12, 26 -- From vapatpxs-at-yahoo.com Mon Apr 20 18:07:43 2009 12, 26 -- Received: from n70.bullet.mail.sp1.yahoo.com (n70.bullet.mail.sp1.yahoo.com [98.136.44.38]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3KN7fxI014910 12, 26 -- for {microscopy-at-microscopy.com} ; Mon, 20 Apr 2009 18:07:42 -0500 12, 26 -- Received: from [216.252.122.217] by n70.bullet.mail.sp1.yahoo.com with NNFMP; 20 Apr 2009 23:07:40 -0000 12, 26 -- Received: from [69.147.65.167] by t2.bullet.sp1.yahoo.com with NNFMP; 20 Apr 2009 23:07:39 -0000 12, 26 -- Received: from [127.0.0.1] by omp502.mail.sp1.yahoo.com with NNFMP; 20 Apr 2009 23:07:39 -0000 12, 26 -- X-Yahoo-Newman-Property: ymail-3 12, 26 -- X-Yahoo-Newman-Id: 962496.36827.bm-at-omp502.mail.sp1.yahoo.com 12, 26 -- Received: (qmail 76532 invoked by uid 60001); 20 Apr 2009 23:07:39 -0000 12, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1240268859; bh=4tHCA3MTjEc9qgiGOPpeM9AC7fBiwjr4kDFgU9hDsoE=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=5AbqQohyAsRNUD2EuEy00KVX6y/Sh1jL4XoZnsax/jE2JVxO/Nyn7Uf5Q7EyiQlkFApQ+q2aH7HJnzLHwD2u7wSPJfJRdD25NY5mmT3woLslH5Bwt/P2HGS6EaBCL7KOBspWXDvFMDE7rZ1i6/xwrS3ZC65MBoS9UgAV7WsuU1M= 12, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 26 -- s=s1024; d=yahoo.com; 12, 26 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; 12, 26 -- b=G9Qa/PqpXTLFeHulLcuc7U8IW1+pOXeS1E5GLleOYz2rEhFn542jhzg0q2tXsldXnRSiGRBIlX6gCggYLSJoZiJNA/yiYzFqnrqL3fcybA1CLcZI/iQZNP9O7WIkFd1sNxeP7GjWXmFAG70EPQ/rdghm5TK+evW5AYjjxwUMPbA=; 12, 26 -- Message-ID: {801623.76495.qm-at-web46101.mail.sp1.yahoo.com} 12, 26 -- X-YMail-OSG: 8ICPzd0VM1kGehoYPWzq5LxJ815hL57J_W5ApI73Iwu8JYF6tUnZsXGIm9lDaPsKQXmjA.ApiIrpHcEH_AMs79cg1i43qs.ew5morVK7DEaecIGzervlhdw.TEsxCK3628Sq6l8w75y2BvWPbaQwl8audKe3af9bYOhTrgV0oIC4cVzL_4y034rA3Kox5JUegh_cHRTRlCGJuH85gZxkMdGvnUjqWBLc5609unafgiP3QEEvpG0XhQTDrGWTgiUbLjQA6IMlpIaTYwloipI- 12, 26 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Mon, 20 Apr 2009 16:07:37 PDT 12, 26 -- X-Mailer: YahooMailClassic/5.2.18 YahooMailWebService/0.7.289.1 12, 26 -- Date: Mon, 20 Apr 2009 16:07:37 -0700 (PDT) 12, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 12, 26 -- Subject: OptiQuip Model 1200 12, 26 -- To: MSA BB {Microscopy-at-microscopy.com} , 12, 26 -- HistoNet {histonet-at-lists.utsouthwestern.edu} 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
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Email: ademp-at-mse.ufl.edu Name: L. Amelia Dempere
Organization: University of Florida
Title-Subject: [Filtered] Certificate in Materials Characterization
Question: This 9-credit online certificate is an opportunity for multidisciplinary scientists and engineers with various educational backgrounds to learn more about current materials characterization techniques. The certificate program is open to all scientists and engineers, with various educational backgrounds. The 3-credit course "Scanning Electron Microscopy and Microanalysis" is offered this summer. The course is designed to introduce the theoretical concepts of Scanning Electron Microscopy (SEM), Electron Probe Microanalysis (EPMA), and Energy Dispersive Spectroscopy (EDS). Additional information at: www.ufedge.eng.ufl.edu
I agree that 100dpi is perfectly fine for poster printing of most images where detail is difficult to discern anyway, say fluorescence in cells or tissues. You do notice low resolution jaggies in the text and in things we are good at discerning, say a microscope, illustrator image or lab view [when we know what they should look like]. Blurred printing on cheap paper [the standard for most poster printing] means that the 600dpi of a modern printer is unlikely ever to be realised. So although we easily spend £2,000+ in man hours producing a poster, we rarely spend more than £40 printing it [and for most purposes this poster resolution is perfectly adequate anyway]. Plus the inks used may be rubbish, our University printed posters have all faded in a month or two after being left up in a windowless lab [obviously not HP inks and HP photographic paper where the inks are guaranteed fast for 100 years]. Fine for posters you use once and bin, but a pain for our constantly re-used & recycled Core Facility poster.
However I would still advise against going to lower than say 1,000x750 for an image whatever size it's going to be printed, if you think you might possibly use it again. Our Core poster has 40+ images going back 5 years, and the 'master' hi-res images are simply lost to history. Probably they were deleted from my processors personal hard drive space when he left, and probably they weren't all ours, but from our collaborators/users. So it is an incredible pain to find that all our images on our main poster ppt files are about 250x300 max pixel size - with multiple backups at different locations, it's often only the poster master ppt file itself that survives. If, at a later date, I want to increase the printed image size from 2x1.5" to say 6x4.5" the pixilation is very noticeable. Plus they look bad in Core PowerPoint and pdf presentations - and we are supposed to be the kings of imaging. You can get by, say by upscaling and re-adding text at higher res - but what a pain, more hours in Photoshop, and with typical day rates of about £400+. And the extra file size of the larger images in the original ppt poster file would have been insignificant on a modern PC.
Keeping track of digital images for the next 30 years is just about impossible, if I want an image from one of my old papers these days I have scan the printed copy. I guess I produce 10,000+ digital images a year at home and at work these days. Backup is one thing, finding a particular image again quickly years on is another matter.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: 16 April 2009 15:39 To: kjmorris-at-well.ox.ac.uk
Our school is using PowerPoint as default software for creating posters for many years.
While 300 ppi is a "golden standard" for printers (glossy magazines), it is surely overkill for posters printed with inkjet printers. From my experience printing images from XL30 SEM (about 1400*1000 pixels) at 11" image size (i.e. 127 ppi) produce good results. Even 100 ppi on poster could be good enough. But for line drawings you need higher resolution.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
I'm sorry if this sounds harsh Keith, but it seems a little odd to me that you use PowerPoint, designed for on-screen presentations, to produce posters.
It is under £120 for you to buy InDesign CS4 (OUCS shop- license and media), and it will save you time as you never have to re-make posters for different sizes, and the process itself is a lot faster. You can probably justify the cost in time savings after only one or two posters.
I know it doesn't address Kristen's question, as she is required to use PowerPoint. For importing into PowerPoint (or any Office application), PNG is the best file type, as it is the native compression for office. I would tend to go for 300ppi, just because computers can cope with large files, but realistically 210ppi is indistinguishable. Don't be confused with printers' dpi (typically inkjet for this application), and pixels per inch. Inkjets can not print continuous tone, they have to place many dots to achieve the colour information contained in a single pixel.
Now to preach on the alternative :)
InDesign links to the original full-resolution version of all images, via the 'place' command, and you can work with thousands of images of very large file sizes without it affecting the speed or reliability of the programme (it is designed for typesetting whole books after all)- this is because it only works with a low-resolution preview of the original file. The InDesign files themselves are never over a few megabytes
You can 'package' all the linked images for a file together in the same place (this duplicates all the linked files and places them together- useful if you have linked to originals in multiple folders spread around your computer).
When you want to have the poster printed, you export as a PDF, down-sampling all the images to the desired resolution and compression in one simple step. The result is a very robust file with all fonts embedded and almost fool-proof for printing, that is never too large or too small for the job.
If you want to edit an image in the poster, you don't have to find your original high resolution file, and make changes, down-sample and re-import, as you would have to do in PowerPoint. Instead, you just right-click, and select 'edit original' from the contextual menu, and it opens the orignal in Photoshop, and after making changes you close the document, and the preview in InDesign will automatically update.
If you ever use vector files, PowerPoint is a nightmare; where as InDesign links directly to Illustrator files, and changes are very quick. And the PDFs created by InDesign will obviously contain perfect copies of the vector information, so even if you do lose all version of a diagram, except a low-resolution PDF of a A0 poster designed for printing A4 as hand-outs, you can open the PDF in Illustrator and copy the perfect diagram out of the file.
These are all in addition to the much more sophisticated text tools (justified text with complete control over the balance between enlarged spaces between words or letters, and hyphenation; word length before hyphenation should occur), automatic alignment tools and the ability to instantly spread items at equal spacing, or a pre-set spacing...
Kind regards,
Ben
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 {http://mrcanu.pharm.ox.ac.uk/}
kjmorris-at-well.ox.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I agree that 100dpi is perfectly fine for poster printing of most images } where detail is difficult to discern anyway, say fluorescence in cells or } tissues. You do notice low resolution jaggies in the text and in things we } are good at discerning, say a microscope, illustrator image or lab view } [when we know what they should look like]. Blurred printing on cheap paper } [the standard for most poster printing] means that the 600dpi of a modern } printer is unlikely ever to be realised. So although we easily spend £2,000+ } in man hours producing a poster, we rarely spend more than £40 printing it } [and for most purposes this poster resolution is perfectly adequate anyway]. } Plus the inks used may be rubbish, our University printed posters have all } faded in a month or two after being left up in a windowless lab [obviously } not HP inks and HP photographic paper where the inks are guaranteed fast for } 100 years]. Fine for posters you use once and bin, but a pain for our } constantly re-used & recycled Core Facility poster. } } However I would still advise against going to lower than say 1,000x750 for } an image whatever size it's going to be printed, if you think you might } possibly use it again. Our Core poster has 40+ images going back 5 years, } and the 'master' hi-res images are simply lost to history. Probably they } were deleted from my processors personal hard drive space when he left, and } probably they weren't all ours, but from our collaborators/users. So it is } an incredible pain to find that all our images on our main poster ppt files } are about 250x300 max pixel size - with multiple backups at different } locations, it's often only the poster master ppt file itself that survives. } If, at a later date, I want to increase the printed image size from 2x1.5" } to say 6x4.5" the pixilation is very noticeable. Plus they look bad in Core } PowerPoint and pdf presentations - and we are supposed to be the kings of } imaging. You can get by, say by upscaling and re-adding text at higher res - } but what a pain, more hours in Photoshop, and with typical day rates of } about £400+. And the extra file size of the larger images in the original } ppt poster file would have been insignificant on a modern PC. } } Keeping track of digital images for the next 30 years is just about } impossible, if I want an image from one of my old papers these days I have } scan the printed copy. I guess I produce 10,000+ digital images a year at } home and at work these days. Backup is one thing, finding a particular image } again quickly years on is another matter. } } Regards } } Keith } } } --------------------------------------------------------------------------- } Dr Keith J. Morris, } Molecular Cytogenetics and Microscopy Core, } Laboratory 00/069 and 00/070, } The Wellcome Trust Centre for Human Genetics, } Roosevelt Drive, } Oxford OX3 7BN, } United Kingdom. } } } Telephone: +44 (0)1865 287568 } Email: kjmorris-at-well.ox.ac.uk } Web-pages: http://www.well.ox.ac.uk/cytogenetics/
==============================Original Headers============================== 17, 28 -- From ben.micklem-at-pharm.ox.ac.uk Tue Apr 21 06:14:47 2009 17, 28 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LBEktC015545 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 06:14:47 -0500 17, 28 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207]) 17, 28 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69) 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 17, 28 -- id 1LwDw6-0002ch-UF 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 +0100 17, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) 17, 28 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 17, 28 -- (Exim 4.69) 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 17, 28 -- id 1LwDw6-0002Oo-3F 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 +0100 17, 28 -- Message-ID: {49EDAAA5.4060508-at-pharm.ox.ac.uk} 17, 28 -- Date: Tue, 21 Apr 2009 12:14:45 +0100 17, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 17, 28 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302) 17, 28 -- MIME-Version: 1.0 17, 28 -- To: microscopy-at-microscopy.com 17, 28 -- Subject: Re: [Microscopy] Advice: Resolution of micrographs for posters? 17, 28 -- References: {200904210934.n3L9YpUM011045-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {200904210934.n3L9YpUM011045-at-ns.microscopy.com} 17, 28 -- X-Enigmail-Version: 0.95.7 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-Oxford-Username: phar0293 ==============================End of - Headers==============================
I have some large (25 - 30cm) type B, C and D, both steel and tungsten carbide, microtome knives that need sharpening/reconditioning.
Does anyone knows a company in Europe that does that kind of work at an affordable price? By the way: what is *normal* price for those things? Impossible to answer question, I suppose...
It struck me that all those microtome knife sharpeners (AO 935, Reichert 903, AO 903...) all look very much the same. Are the glass plates interchangeable?
Would the glass plates for the current Leica sharpener SP9000 (part.no. 14041819698, dimensions 292mm x 127mm x 6 mm) fit the Reichert Microtome Knife Sharpener 903?
It would be great if someone owning a model 903 could measure the plates :-).
Just thought I'd let you know that your nice write up on the advantages of InDesign has been appreciated! That does sound like the right way to keep your posters and related stuff organized. One of these days...
On the meetings I have been too, though, your InDesign-made would be in a small minority, so please don't be too harsh on Keith... By far the majority of those posters I've seen have been done in PowerPoint. From that perspective, doing your poster in PP today is hardly "odd", although it is of course not the optimal way.
Now back to being serious and constructive - this does sound like something people who make posters should be more aware of. Perhaps we could ask those Adobe representatives ("evangelists") to some tutorials during microscopy meetings? (I am thinking about M&M meetings in particular, but there are of course others...)
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
} I'm sorry if this sounds harsh Keith, but it seems a little odd to me } that you use PowerPoint, designed for on-screen presentations, to } produce posters. } } It is under £120 for you to buy InDesign CS4 (OUCS shop- license and } media), and it will save you time as you never have to re-make posters } for different sizes, and the process itself is a lot faster. You can } probably justify the cost in time savings after only one or two } posters. } } I know it doesn't address Kristen's question, as she is required to } use } PowerPoint. For importing into PowerPoint (or any Office application), } PNG is the best file type, as it is the native compression for } office. } I would tend to go for 300ppi, just because computers can cope with } large files, but realistically 210ppi is indistinguishable. Don't be } confused with printers' dpi (typically inkjet for this application), } and } pixels per inch. Inkjets can not print continuous tone, they have to } place many dots to achieve the colour information contained in a } single } pixel. } } } } Now to preach on the alternative :) } } InDesign links to the original full-resolution version of all images, } via the 'place' command, and you can work with thousands of images of } very large file sizes without it affecting the speed or reliability of } the programme (it is designed for typesetting whole books after all)- } this is because it only works with a low-resolution preview of the } original file. The InDesign files themselves are never over a few } megabytes } } You can 'package' all the linked images for a file together in the } same } place (this duplicates all the linked files and places them together- } useful if you have linked to originals in multiple folders spread } around } your computer). } } When you want to have the poster printed, you export as a PDF, } down-sampling all the images to the desired resolution and compression } in one simple step. The result is a very robust file with all fonts } embedded and almost fool-proof for printing, that is never too large } or } too small for the job. } } If you want to edit an image in the poster, you don't have to find } your } original high resolution file, and make changes, down-sample and } re-import, as you would have to do in PowerPoint. Instead, you just } right-click, and select 'edit original' from the contextual menu, } and it } opens the orignal in Photoshop, and after making changes you close the } document, and the preview in InDesign will automatically update. } } If you ever use vector files, PowerPoint is a nightmare; where as } InDesign links directly to Illustrator files, and changes are very } quick. And the PDFs created by InDesign will obviously contain perfect } copies of the vector information, so even if you do lose all version } of } a diagram, except a low-resolution PDF of a A0 poster designed for } printing A4 as hand-outs, you can open the PDF in Illustrator and copy } the perfect diagram out of the file. } } These are all in addition to the much more sophisticated text tools } (justified text with complete control over the balance between } enlarged } spaces between words or letters, and hyphenation; word length before } hyphenation should occur), automatic alignment tools and the ability } to } instantly spread items at equal spacing, or a pre-set spacing... } } Kind regards, } } Ben } } } -- } Imaging Technician } MRC Anatomical Neuropharmacology Unit, Mansfield Road, } Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 } {http://mrcanu.pharm.ox.ac.uk/} } } kjmorris-at-well.ox.ac.uk wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } I agree that 100dpi is perfectly fine for poster printing of most } } images } } where detail is difficult to discern anyway, say fluorescence in } } cells or } } tissues. You do notice low resolution jaggies in the text and in } } things we } } are good at discerning, say a microscope, illustrator image or lab } } view } } [when we know what they should look like]. Blurred printing on } } cheap paper } } [the standard for most poster printing] means that the 600dpi of a } } modern } } printer is unlikely ever to be realised. So although we easily } } spend £2,000+ } } in man hours producing a poster, we rarely spend more than £40 } } printing it } } [and for most purposes this poster resolution is perfectly adequate } } anyway]. } } Plus the inks used may be rubbish, our University printed posters } } have all } } faded in a month or two after being left up in a windowless lab } } [obviously } } not HP inks and HP photographic paper where the inks are guaranteed } } fast for } } 100 years]. Fine for posters you use once and bin, but a pain for our } } constantly re-used & recycled Core Facility poster. } } } } However I would still advise against going to lower than say } } 1,000x750 for } } an image whatever size it's going to be printed, if you think you } } might } } possibly use it again. Our Core poster has 40+ images going back 5 } } years, } } and the 'master' hi-res images are simply lost to history. Probably } } they } } were deleted from my processors personal hard drive space when he } } left, and } } probably they weren't all ours, but from our collaborators/users. } } So it is } } an incredible pain to find that all our images on our main poster } } ppt files } } are about 250x300 max pixel size - with multiple backups at different } } locations, it's often only the poster master ppt file itself that } } survives. } } If, at a later date, I want to increase the printed image size from } } 2x1.5" } } to say 6x4.5" the pixilation is very noticeable. Plus they look bad } } in Core } } PowerPoint and pdf presentations - and we are supposed to be the } } kings of } } imaging. You can get by, say by upscaling and re-adding text at } } higher res - } } but what a pain, more hours in Photoshop, and with typical day } } rates of } } about £400+. And the extra file size of the larger images in the } } original } } ppt poster file would have been insignificant on a modern PC. } } } } Keeping track of digital images for the next 30 years is just about } } impossible, if I want an image from one of my old papers these days } } I have } } scan the printed copy. I guess I produce 10,000+ digital images a } } year at } } home and at work these days. Backup is one thing, finding a } } particular image } } again quickly years on is another matter. } } } } Regards } } } } Keith } } } } } } --------------------------------------------------------------------------- } } Dr Keith J. Morris, } } Molecular Cytogenetics and Microscopy Core, } } Laboratory 00/069 and 00/070, } } The Wellcome Trust Centre for Human Genetics, } } Roosevelt Drive, } } Oxford OX3 7BN, } } United Kingdom. } } } } } } Telephone: +44 (0)1865 287568 } } Email: kjmorris-at-well.ox.ac.uk } } Web-pages: http://www.well.ox.ac.uk/cytogenetics/ } } ==============================Original } Headers============================== } 17, 28 -- From ben.micklem-at-pharm.ox.ac.uk Tue Apr 21 06:14:47 2009 } 17, 28 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk } [163.1.2.169]) } 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n3LBEktC015545 } 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 } 06:14:47 -0500 } 17, 28 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207]) } 17, 28 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69) } 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) } 17, 28 -- id 1LwDw6-0002ch-UF } 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 } +0100 } 17, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) } 17, 28 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) } 17, 28 -- (Exim 4.69) } 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) } 17, 28 -- id 1LwDw6-0002Oo-3F } 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 } +0100 } 17, 28 -- Message-ID: {49EDAAA5.4060508-at-pharm.ox.ac.uk} } 17, 28 -- Date: Tue, 21 Apr 2009 12:14:45 +0100 } 17, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} } 17, 28 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302) } 17, 28 -- MIME-Version: 1.0 } 17, 28 -- To: microscopy-at-microscopy.com } 17, 28 -- Subject: Re: [Microscopy] Advice: Resolution of } micrographs for posters? } 17, 28 -- References: {200904210934.n3L9YpUM011045-at-ns.microscopy.com} } 17, 28 -- In-Reply-To: {200904210934.n3L9YpUM011045-at-ns.microscopy.com} } 17, 28 -- X-Enigmail-Version: 0.95.7 } 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1 } 17, 28 -- Content-Transfer-Encoding: 8bit } 17, 28 -- X-Oxford-Username: phar0293 } ==============================End of - } Headers==============================
==============================Original Headers============================== 11, 24 -- From vladislav_speransky-at-nih.gov Tue Apr 21 16:17:32 2009 11, 24 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LLHMgC024307 11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 16:17:32 -0500 11, 24 -- X-IronPortListener: NIH_Relay 11, 24 -- X-SBRS: None 11, 24 -- X-IronPort-AV: E=Sophos;i="4.40,226,1238990400"; 11, 24 -- d="scan'208";a="86558312" 11, 24 -- Received: from helix.nih.gov ([128.231.2.3]) 11, 24 -- by nihrelayxway2.hub.nih.gov with ESMTP; 21 Apr 2009 17:17:17 -0400 11, 24 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185]) 11, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id n3LLHGf4031525 11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 17:17:16 -0400 11, 24 -- Message-Id: {6FC32721-A969-4A83-A9D1-27B1EA35F08F-at-nih.gov} 11, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 11, 24 -- To: Microscopy-at-microscopy.com 11, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 11, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 24 -- Subject: Fwd: [Microscopy] Re: Advice: Resolution of micrographs for posters? 11, 24 -- Date: Tue, 21 Apr 2009 17:17:15 -0400 11, 24 -- References: {D13763A4740B07428566B25D7E768DA69F1C1AB2-at-NIHMLBX02.nih.gov} 11, 24 -- X-Mailer: Apple Mail (2.930.3) 11, 24 -- Content-Transfer-Encoding: 8bit 11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3LLHMgC024307 ==============================End of - Headers==============================
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As Vlad, Ben and others have commented, the BEST program is not always the one being used to make posters. In our central facility, we produce many hundreds of posters a year. The vast majority are produced using PowerPoint, or, as our graphics person calls it, ProblemPoint. If done properly (a rare event), the results can be as good as those obtained using InDesign. However, when problems are encountered, it takes a long time to figure out why PP is not producing posters that print properly. We only rarely have this problem with InDesign.
We have been using InDesign (and previously PageMaker, Quark and Illustrator) to produce posters over the years, but when researchers bring in "pre-made" posters, it is nearly aways a PP job. When problems are encountered (usually due to the Postscript printer balking at the PP hackjob), the harried researchers want to know "the best" program to use. We always recommend they purchase the Adobe Design Suite CS4. More and more researchers are doing this and the word is slowly getting around. It will take time and patience.
This summer we will be giving a series of training sessions on how to produce high quality posters using the CS4 Design Suite (Photoshop, Illustrator, InDesign and Acrobat). We'll see if this improves the situation. I believe it will.
JB
} Just thought I'd let you know that your nice write up on the } advantages of InDesign has been appreciated! That does sound like the } right way to keep your posters and related stuff organized. One of } these days... } } On the meetings I have been too, though, your InDesign-made would be } in a small minority, so please don't be too harsh on Keith... By far } the majority of those posters I've seen have been done in PowerPoint. } From that perspective, doing your poster in PP today is hardly "odd", } although it is of course not the optimal way. } } Now back to being serious and constructive - this does sound like } something people who make posters should be more aware of. Perhaps we } could ask those Adobe representatives ("evangelists") to some } tutorials during microscopy meetings? (I am thinking about M&M } meetings in particular, but there are of course others...)
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Our core poster was originally created in Powerpoint [not by me I might add]. I had a go at converting it to Indesign CS3 but after an hour or so I lost the will to live. Not helped by Adobe's obtuse help [I'm sure Adobe is a front for the Bill Gate's evil empire, created simply to make Microsoft software look good]. Adobe's stuff is buggy as well on first release. With our Core poster, we only have to change the odd staff photo mainly, as predecessor's relocate to the Solyent Green factory. Plus of course all the images are mostly stuck at 250x300 resolution anyway and our ppt file always prints OK [so if it's not broke..]. Indesign is much easier if you use it to create the poster from scratch.
Having spent the last year wrestling with *Flash CS3's incomprehensible help [often not even giving info on the right program], it doesn't make Indesign that attractive for very occasional use - if you want to see how easy to use a DTP program can be, try Serif's PagePlus X3, it makes Publisher look pants [well I suppose Publisher is pants]. Trouble is the learning curve on Adobe software is so steep you really have to use it every day to become comfortable, not once a year. Quark Express is no better in terms of intuitive use either. I did get to grips with GoLive, then Adobe killed it off after buying MacroMedia. I use Illustrator occasionally, but mostly it's Photoshop, Flash, Acrobat Pro, Dreamweaver and PhotoShop [again & again]. I know Photoshop so well now I have fooled myself into thinking it's easy to use. Besides it's Mat Lab and Dragon Naturally Speaking 10 that on my to-do list for this month - plus of course Microsoft's 'intuitive' new ribbon interface [I have kept Office 2003 installed with Office 2007 - easy to do, with a few mods].
That said Adobe on-line video help is very very useful [but it's only available for the latest products]. Try:
http://www.adobe.com/designcenter/video_workshop/?id=vid0118 Adobe Video Workshop
Plus we have our local OUCS courses and there's Amazon for a selection of books.
Keith
*Are you unique? Why not find out http://uniqueness.well.ox.ac.uk/language_set/introduction.php
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: 21 April 2009 22:58 To: kjmorris-at-well.ox.ac.uk
Ben,
Just thought I'd let you know that your nice write up on the advantages of InDesign has been appreciated! That does sound like the right way to keep your posters and related stuff organized. One of these days...
On the meetings I have been too, though, your InDesign-made would be in a small minority, so please don't be too harsh on Keith... By far the majority of those posters I've seen have been done in PowerPoint. From that perspective, doing your poster in PP today is hardly "odd", although it is of course not the optimal way.
Now back to being serious and constructive - this does sound like something people who make posters should be more aware of. Perhaps we could ask those Adobe representatives ("evangelists") to some tutorials during microscopy meetings? (I am thinking about M&M meetings in particular, but there are of course others...)
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
} I'm sorry if this sounds harsh Keith, but it seems a little odd to me } that you use PowerPoint, designed for on-screen presentations, to } produce posters. } } It is under £120 for you to buy InDesign CS4 (OUCS shop- license and } media), and it will save you time as you never have to re-make posters } for different sizes, and the process itself is a lot faster. You can } probably justify the cost in time savings after only one or two } posters. } } I know it doesn't address Kristen's question, as she is required to } use } PowerPoint. For importing into PowerPoint (or any Office application), } PNG is the best file type, as it is the native compression for } office. } I would tend to go for 300ppi, just because computers can cope with } large files, but realistically 210ppi is indistinguishable. Don't be } confused with printers' dpi (typically inkjet for this application), } and } pixels per inch. Inkjets can not print continuous tone, they have to } place many dots to achieve the colour information contained in a } single } pixel. } } } } Now to preach on the alternative :) } } InDesign links to the original full-resolution version of all images, } via the 'place' command, and you can work with thousands of images of } very large file sizes without it affecting the speed or reliability of } the programme (it is designed for typesetting whole books after all)- } this is because it only works with a low-resolution preview of the } original file. The InDesign files themselves are never over a few } megabytes } } You can 'package' all the linked images for a file together in the } same } place (this duplicates all the linked files and places them together- } useful if you have linked to originals in multiple folders spread } around } your computer). } } When you want to have the poster printed, you export as a PDF, } down-sampling all the images to the desired resolution and compression } in one simple step. The result is a very robust file with all fonts } embedded and almost fool-proof for printing, that is never too large } or } too small for the job. } } If you want to edit an image in the poster, you don't have to find } your } original high resolution file, and make changes, down-sample and } re-import, as you would have to do in PowerPoint. Instead, you just } right-click, and select 'edit original' from the contextual menu, } and it } opens the orignal in Photoshop, and after making changes you close the } document, and the preview in InDesign will automatically update. } } If you ever use vector files, PowerPoint is a nightmare; where as } InDesign links directly to Illustrator files, and changes are very } quick. And the PDFs created by InDesign will obviously contain perfect } copies of the vector information, so even if you do lose all version } of } a diagram, except a low-resolution PDF of a A0 poster designed for } printing A4 as hand-outs, you can open the PDF in Illustrator and copy } the perfect diagram out of the file. } } These are all in addition to the much more sophisticated text tools } (justified text with complete control over the balance between } enlarged } spaces between words or letters, and hyphenation; word length before } hyphenation should occur), automatic alignment tools and the ability } to } instantly spread items at equal spacing, or a pre-set spacing... } } Kind regards, } } Ben } } } -- } Imaging Technician } MRC Anatomical Neuropharmacology Unit, Mansfield Road, } Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271867 } {http://mrcanu.pharm.ox.ac.uk/} } } kjmorris-at-well.ox.ac.uk wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } I agree that 100dpi is perfectly fine for poster printing of most } } images } } where detail is difficult to discern anyway, say fluorescence in } } cells or } } tissues. You do notice low resolution jaggies in the text and in } } things we } } are good at discerning, say a microscope, illustrator image or lab } } view } } [when we know what they should look like]. Blurred printing on } } cheap paper } } [the standard for most poster printing] means that the 600dpi of a } } modern } } printer is unlikely ever to be realised. So although we easily } } spend £2,000+ } } in man hours producing a poster, we rarely spend more than £40 } } printing it } } [and for most purposes this poster resolution is perfectly adequate } } anyway]. } } Plus the inks used may be rubbish, our University printed posters } } have all } } faded in a month or two after being left up in a windowless lab } } [obviously } } not HP inks and HP photographic paper where the inks are guaranteed } } fast for } } 100 years]. Fine for posters you use once and bin, but a pain for our } } constantly re-used & recycled Core Facility poster. } } } } However I would still advise against going to lower than say } } 1,000x750 for } } an image whatever size it's going to be printed, if you think you } } might } } possibly use it again. Our Core poster has 40+ images going back 5 } } years, } } and the 'master' hi-res images are simply lost to history. Probably } } they } } were deleted from my processors personal hard drive space when he } } left, and } } probably they weren't all ours, but from our collaborators/users. } } So it is } } an incredible pain to find that all our images on our main poster } } ppt files } } are about 250x300 max pixel size - with multiple backups at different } } locations, it's often only the poster master ppt file itself that } } survives. } } If, at a later date, I want to increase the printed image size from } } 2x1.5" } } to say 6x4.5" the pixilation is very noticeable. Plus they look bad } } in Core } } PowerPoint and pdf presentations - and we are supposed to be the } } kings of } } imaging. You can get by, say by upscaling and re-adding text at } } higher res - } } but what a pain, more hours in Photoshop, and with typical day } } rates of } } about £400+. And the extra file size of the larger images in the } } original } } ppt poster file would have been insignificant on a modern PC. } } } } Keeping track of digital images for the next 30 years is just about } } impossible, if I want an image from one of my old papers these days } } I have } } scan the printed copy. I guess I produce 10,000+ digital images a } } year at } } home and at work these days. Backup is one thing, finding a } } particular image } } again quickly years on is another matter. } } } } Regards } } } } Keith } } } } } } --------------------------------------------------------------------------- } } Dr Keith J. Morris, } } Molecular Cytogenetics and Microscopy Core, } } Laboratory 00/069 and 00/070, } } The Wellcome Trust Centre for Human Genetics, } } Roosevelt Drive, } } Oxford OX3 7BN, } } United Kingdom. } } } } } } Telephone: +44 (0)1865 287568 } } Email: kjmorris-at-well.ox.ac.uk } } Web-pages: http://www.well.ox.ac.uk/cytogenetics/ } } ==============================Original } Headers============================== } 17, 28 -- From ben.micklem-at-pharm.ox.ac.uk Tue Apr 21 06:14:47 2009 } 17, 28 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk } [163.1.2.169]) } 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n3LBEktC015545 } 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 } 06:14:47 -0500 } 17, 28 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207]) } 17, 28 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.69) } 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) } 17, 28 -- id 1LwDw6-0002ch-UF } 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 } +0100 } 17, 28 -- Received: from pa-s02.mrc.ox.ac.uk ([163.1.195.12]) } 17, 28 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) } 17, 28 -- (Exim 4.69) } 17, 28 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) } 17, 28 -- id 1LwDw6-0002Oo-3F } 17, 28 -- for microscopy-at-microscopy.com; Tue, 21 Apr 2009 12:14:46 } +0100 } 17, 28 -- Message-ID: {49EDAAA5.4060508-at-pharm.ox.ac.uk} } 17, 28 -- Date: Tue, 21 Apr 2009 12:14:45 +0100 } 17, 28 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} } 17, 28 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302) } 17, 28 -- MIME-Version: 1.0 } 17, 28 -- To: microscopy-at-microscopy.com } 17, 28 -- Subject: Re: [Microscopy] Advice: Resolution of } micrographs for posters? } 17, 28 -- References: {200904210934.n3L9YpUM011045-at-ns.microscopy.com} } 17, 28 -- In-Reply-To: {200904210934.n3L9YpUM011045-at-ns.microscopy.com} } 17, 28 -- X-Enigmail-Version: 0.95.7 } 17, 28 -- Content-Type: text/plain; charset=ISO-8859-1 } 17, 28 -- Content-Transfer-Encoding: 8bit } 17, 28 -- X-Oxford-Username: phar0293 } ==============================End of - } Headers==============================
==============================Original Headers============================== 11, 24 -- From vladislav_speransky-at-nih.gov Tue Apr 21 16:17:32 2009 11, 24 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3LLHMgC024307 11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 16:17:32 -0500 11, 24 -- X-IronPortListener: NIH_Relay 11, 24 -- X-SBRS: None 11, 24 -- X-IronPort-AV: E=Sophos;i="4.40,226,1238990400"; 11, 24 -- d="scan'208";a="86558312" 11, 24 -- Received: from helix.nih.gov ([128.231.2.3]) 11, 24 -- by nihrelayxway2.hub.nih.gov with ESMTP; 21 Apr 2009 17:17:17 -0400 11, 24 -- Received: from db4185.niaid.nih.gov (db4185.niaid.nih.gov [128.231.217.185]) 11, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id n3LLHGf4031525 11, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Apr 2009 17:17:16 -0400 11, 24 -- Message-Id: {6FC32721-A969-4A83-A9D1-27B1EA35F08F-at-nih.gov} 11, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 11, 24 -- To: Microscopy-at-microscopy.com 11, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 11, 24 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 24 -- Subject: Fwd: [Microscopy] Re: Advice: Resolution of micrographs for posters? 11, 24 -- Date: Tue, 21 Apr 2009 17:17:15 -0400 11, 24 -- References: {D13763A4740B07428566B25D7E768DA69F1C1AB2-at-NIHMLBX02.nih.gov} 11, 24 -- X-Mailer: Apple Mail (2.930.3) 11, 24 -- Content-Transfer-Encoding: 8bit 11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3LLHMgC024307 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 23 -- From kjmorris-at-well.ox.ac.uk Wed Apr 22 05:29:09 2009 26, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 26, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3MAT6Cr032403 26, 23 -- for {microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 05:29:08 -0500 26, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 26, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 26, 23 -- id 1LwZhQ-0007fd-AV 26, 23 -- for microscopy-at-microscopy.com; Wed, 22 Apr 2009 11:29:04 +0100 26, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 26, 23 -- To: {microscopy-at-microscopy.com} 26, 23 -- References: {200904212158.n3LLwGVX016336-at-ns.microscopy.com} 26, 23 -- Subject: RE: [Microscopy] Fwd: Re: Advice: Resolution of micrographs for posters? 26, 23 -- Date: Wed, 22 Apr 2009 11:29:04 +0100 26, 23 -- Message-ID: {FD2979C9F8BC47AC9477225BE88EF942-at-CytoWhizz} 26, 23 -- MIME-Version: 1.0 26, 23 -- Content-Type: text/plain; 26, 23 -- charset="iso-8859-1" 26, 23 -- X-Mailer: Microsoft Office Outlook 11 26, 23 -- In-Reply-To: {200904212158.n3LLwGVX016336-at-ns.microscopy.com} 26, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 26, 23 -- thread-index: AcnCzEyKk0r+QyFXTf+UWzwD1PhRiQAYb6fA 26, 23 -- Content-Transfer-Encoding: 8bit 26, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3MAT6Cr032403 ==============================End of - Headers==============================
We need to purchase a demagnetizer, to use mostly for forceps (handling nickel grids, etc). Not having any experience with these, I'd like some opinions. We'd like to purchase the less expensive one, but not if the general opinion is that it's not effective.
Ted Pella's runs $85-90 (http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS' costs about $255 (http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as px#62083).
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
Alternatively, depending on how well de-magnetizers work, you could buy anti-magnetic forceps. I've no experience of de-magnetizers, but our anti-mag, anti capillary forceps (we got ours from TAAB www.taab.co.uk ) have stood the test of time working extensively with nickel grids.
Alastair McKinnon IMS Histology & EM Facility Manager University of Aberdeen, Institute of Medical Sciences Foresterhill, Aberdeen, AB25 2ZD 01224 552923; www.abdn.ac.uk/ims/h-em/
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: 22 April 2009 23:48 To: Mckinnon, Alastair D.
We need to purchase a demagnetizer, to use mostly for forceps (handling nickel grids, etc). Not having any experience with these, I'd like some opinions. We'd like to purchase the less expensive one, but not if the general opinion is that it's not effective.
Ted Pella's runs $85-90 (http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS' costs about $255 (http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as px#62083).
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
Jaci, I've had several of an older version of the Ladd demagnetizer http://www.laddresearch.com/General_Catalog/Chapter_7/Small_Equipment___Inst ruments/Miscellaneous_Small_Equipment/Demagnetizer/demagnetizer.html which looks very much like the Pella. I've been quite happy over the past 28 years. Oh, I had several because I had several field engineers at one point. I'm still using the first one I bought.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Wednesday, April 22, 2009 6:46 PM To: kenconverse-at-qualityimages.biz
We need to purchase a demagnetizer, to use mostly for forceps (handling nickel grids, etc). Not having any experience with these, I'd like some opinions. We'd like to purchase the less expensive one, but not if the general opinion is that it's not effective.
Ted Pella's runs $85-90 (http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS' costs about $255 (http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as px#62083).
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
We have the unit from Pella. This works very effectively on standard forceps (or other tools) and allows one to either magnetize or demagnetize by choice of which direction the forceps pass into the loop; for some purposes having tools be magnetic is an advantage....
Non-magnetic forceps are nice also, but I believe that I read that the steel is not as hard as the standard grades of the same manufacturer - one of the suppliers (EMS?) had a lot of info at their website on the styles/materials of the Dumont line.
Dale
a.d.mckinnon-at-abdn.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Alternatively, depending on how well de-magnetizers work, you could buy anti-magnetic forceps. I've no experience of de-magnetizers, but our anti-mag, anti capillary forceps (we got ours from TAAB www.taab.co.uk ) have stood the test of time working extensively with nickel grids. } } } Alastair McKinnon } IMS Histology & EM Facility Manager } University of Aberdeen, } Institute of Medical Sciences } Foresterhill, Aberdeen, AB25 2ZD } 01224 552923; www.abdn.ac.uk/ims/h-em/ } } -----Original Message----- } X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] } Sent: 22 April 2009 23:48 } To: Mckinnon, Alastair D. } Subject: [Microscopy] TEM: demagnetizers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We need to purchase a demagnetizer, to use mostly for forceps (handling nickel grids, etc). Not having any experience with these, I'd like some opinions. We'd like to purchase the less expensive one, but not if the general opinion is that it's not effective. } } Ted Pella's runs $85-90 } (http://www.tedpella.com/tools_html/tool1.htm#anchor368274) and EMS' } costs about $255 } (http://www.emsdiasum.com/microscopy/products/tweezers/forceps_warmer.as } px#62083). } } Thank you, } } Jaci } } Jaclynn Lett } Senior Research Technician, EM Facility } Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110 } } Email: lettj-at-ent.wustl.edu } http://otocore.wustl.edu } } } } ==============================Original Headers============================== } 8, 23 -- From LettJ-at-ent.wustl.edu Wed Apr 22 17:42:14 2009 8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu [128.252.17.171]) } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3MMgEZs012661 } 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Apr 2009 17:42:14 -0500 } 8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 23 -- Wed, 22 Apr 2009 17:42:10 -0500 } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; } 8, 23 -- charset="us-ascii" } 8, 23 -- Subject: TEM: demagnetizers } 8, 23 -- Date: Wed, 22 Apr 2009 17:42:10 -0500 8, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C39CB45FD-at-EX04.wusm-pcf.wustl.edu} } 8, 23 -- X-MS-Has-Attach: } 8, 23 -- X-MS-TNEF-Correlator: } 8, 23 -- Thread-Topic: TEM: demagnetizers 8, 23 -- Thread-Index: AcnDm5IKJP+9b4MVS7KEH1yCTDyj9g== 8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 23 -- To: {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 22 Apr 2009 22:42:10.0669 (UTC) FILETIME=[92C7E1D0:01C9C39B] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3MMgEZs012661 ==============================End of - Headers============================== } } } The University of Aberdeen is a charity registered in Scotland, No SC013683. } } } ==============================Original Headers============================== } 20, 29 -- From a.d.mckinnon-at-abdn.ac.uk Thu Apr 23 03:24:40 2009 } 20, 29 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk [139.133.7.8]) } 20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3N8OdCo011457 } 20, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 03:24:40 -0500 } 20, 29 -- Received: from ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83] helo=mail.abdn.ac.uk) } 20, 29 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52) } 20, 29 -- id 1LwuEY-0001Ls-Ql; Thu, 23 Apr 2009 09:24:38 +0100 } 20, 29 -- Received: from VMAILB.uoa.abdn.ac.uk ([139.133.15.92]) by } 20, 29 -- ew-mail-3.uoa.abdn.ac.uk ([139.133.15.83]) with mapi; Thu, 23 Apr 2009 } 20, 29 -- 09:24:38 +0100 } 20, 29 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} } 20, 29 -- To: "'LettJ-at-ent.wustl.edu'" {LettJ-at-ent.wustl.edu} } 20, 29 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 20, 29 -- Date: Thu, 23 Apr 2009 09:24:38 +0100 } 20, 29 -- Subject: RE: [Microscopy] TEM: demagnetizers } 20, 29 -- Thread-Topic: [Microscopy] TEM: demagnetizers } 20, 29 -- Thread-Index: AcnDnHRfRdrGbDz3Soa4Bx76Z6CFXwATuUUg } 20, 29 -- Message-ID: {A3317CA649BC8C4998EED327D66A8D95B346300E5A-at-VMAILB.uoa.abdn.ac.uk} } 20, 29 -- References: {200904222248.n3MMmEIl019527-at-ns.microscopy.com} } 20, 29 -- In-Reply-To: {200904222248.n3MMmEIl019527-at-ns.microscopy.com} } 20, 29 -- Accept-Language: en-US } 20, 29 -- Content-Language: en-US } 20, 29 -- X-MS-Has-Attach: } 20, 29 -- X-MS-TNEF-Correlator: } 20, 29 -- acceptlanguage: en-US } 20, 29 -- Content-Type: text/plain; charset="us-ascii" } 20, 29 -- MIME-Version: 1.0 } 20, 29 -- Content-Transfer-Encoding: 8bit } 20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3N8OdCo011457 } ==============================End of - Headers==============================
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I'm a graduate student at Materials Science Program. I tried to section a poly(4-vinylpyridine) (P4VP) embedded in epon with a diamond knife and water on the boat. However, it seemed that the water ate away my sample and the knife only cut the epon. If i looked at the sample face after trying to section, i could see that the sample face had become rough, which i assumed because the water somehow dissolving the sample (In large amount, P4VP is insoluble in water). Would you please suggest me how to resolve this problem? Thank you very much.
Sincerely, Melvina Leolukman
==============================Original Headers============================== 3, 33 -- From leolukman-at-wisc.edu Thu Apr 23 13:21:09 2009 3, 33 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3NIL85O027207 3, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr 2009 13:21:08 -0500 3, 33 -- MIME-version: 1.0 3, 33 -- Content-transfer-encoding: 7BIT 3, 33 -- Content-disposition: inline 3, 33 -- Content-type: text/plain; charset=us-ascii 3, 33 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 3, 33 -- smtpauth1.wiscmail.wisc.edu 3, 33 -- (Sun Java(tm) System Messaging Server 7.0-5.01 32bit (built Feb 19 2009)) 3, 33 -- id {0KIK00E02GB8KN00-at-smtpauth1.wiscmail.wisc.edu} for 3, 33 -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 13:21:08 -0500 (CDT) 3, 33 -- Received: from wiscmail.wisc.edu (store1.doit.wisc.edu [144.92.8.164]) 3, 33 -- by smtpauth1.wiscmail.wisc.edu 3, 33 -- (Sun Java(tm) System Messaging Server 7.0-5.01 32bit (built Feb 19 2009)) 3, 33 -- with ESMTP id {0KIK009W9GB76120-at-smtpauth1.wiscmail.wisc.edu} for 3, 33 -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 13:21:07 -0500 (CDT) 3, 33 -- Received: from [144.92.8.222] (Forwarded-For: 128.104.185.23, [144.92.197.247]) 3, 33 -- by store1.doit.wisc.edu (mshttpd); Thu, 23 Apr 2009 13:21:07 -0500 3, 33 -- Date: Thu, 23 Apr 2009 13:21:07 -0500 3, 33 -- From: MELVINA LEOLUKMAN {leolukman-at-wisc.edu} 3, 33 -- Subject: sectioning p4vp 3, 33 -- To: Microscopy-at-microscopy.com 3, 33 -- Message-id: {e51c89d31f6ec.49f06b43-at-wiscmail.wisc.edu} 3, 33 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-8.04 (built Feb 28 2007) 3, 33 -- Content-language: en 3, 33 -- X-Accept-Language: en 3, 33 -- Priority: normal 3, 33 -- X-Spam-Report: TrustedSender=yes, SenderIP=144.92.8.164 3, 33 -- X-Spam-PmxInfo: Server=avs-10, Version=5.5.1.360522, 3, 33 -- Antispam-Engine: 2.6.1.350677, Antispam-Data: 2009.4.23.180432, 3, 33 -- SenderIP=144.92.8.164 ==============================End of - Headers==============================
Colleagues, my (genial) service-technician wants to buy a Reichert Ultracut "E" or "S". If anybody wants to sell a surplus / unused / used Ultracut E or Ultracut S in Europe, please contact Mr Volker Busse directly by mail Techno-Med-at-t-online.de
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both aforlenza-at-nikon.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] 2009 Nikon International Small World Competition
Question: Have a beautiful photomicrograph you would like to share with the world? Nikon invites you to showcase your photomicrographs in the 2009 Nikon International Small World Competition. Every year, Nikon receives beautiful images for submission and would love to view yours this year. Last year the winning photographers and their images were viewed by over 39 million people! The subject matter is unrestricted and any light microscopy technique is acceptable. This is the perfect format for the scientific community to share their science and creative eye with the world. The deadline of April 30, 2009 is quickly approaching, so hurry to www.nikonsmallworld.com for entry and contest rules. Receive a free Small World calendar just for entering!
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Email: hagglundk1-at-nku.edu Name: Karl Hagglund
Title-Subject: [Filtered] Maltese Cross in polarized light microscopy
Question: I have a customer who has asked about the formation of the maltese cross interference pattern under crossed polarized light. A common example of this can be seen in a variety of starches viewed under crossed polars.
Can someone provide a concise explanation or a good reference that explains why we see the maltese cross?
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Organization: Institute of Cell biology and Pathology, Charles Uni, Prague
Title-Subject: [Filtered] looking for a Zeiss 902 trackball
Question: Dear Fellow Microscopists,
we have a Zeiss TEM 902 that we would like to keep in operation for students. However, we are missing a functional trackball, which is also not available from the service. If you come across one in your lab's cellar, please do not throw it away but let us know!
With best regards,
Lubomir Kovacik
Institute of Cellular Biology and Pathology 1st Faculty of Medicine, Charles University Prague Czech Republic
Starch molecules in the starch grains are laid down in concentric layers. This arrangement gives rise to form birefringence. The same would be true form molecules in a radial arrangement. Both arrangements will rotate the incident polarized light beam so that the light will pass the second polarizer (analyzer) in these quadrants, giving the "maltese cross". Additionally, by using an appropriate "compensator plate", one can observe "addition and subtraction colors" in the orthogonal regions of brightness and distinguish the circumferential from the radial arrangement.
There is a wealth of detailed information at: http://micro.magnet.fsu.edu/primer/techniques/polarized/polarizedhome.html
We have a book in our library with an excellent article by H. Stanley Bennett on polarized light microscopy. I think this is the book: McClung's handbook of microscopial technique for workers in animal and plant tissues, by thirty-five authors. Edited by Ruth McClung Jones. New York, Hafner Pub. Co. [1964, c1950] xix, 790 p. illus., diagrs. 24 cm.
If this isn't the correct reference I will track it down for you. The Molecular Expressions information (link above) should have all you need.
Hope this helps.
Dale
hagglundk1-at-nku.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both hagglundk1-at-nku.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: hagglundk1-at-nku.edu } Name: Karl Hagglund } } Title-Subject: [Filtered] Maltese Cross in polarized light microscopy } } Question: I have a customer who has asked about the formation of the } maltese cross interference pattern under crossed polarized light. A } common example of this can be seen in a variety of starches viewed } under crossed polars. } } Can someone provide a concise explanation or a good reference that } explains why we see the maltese cross? } } Thanks in advance, } } Karl } } Login Host: 199.64.0.252 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:25 2009 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ODKOSr003559 } 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:20:25 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c6176d07521b-at-[206.69.208.22]} } 8, 11 -- Date: Fri, 24 Apr 2009 08:20:23 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: hagglundk1-at-nku.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Maltese Cross in polarized light microscopy } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
Hello Karl, The Maltese cross, is in my opinion, is one of coolest things you can find by PLM. Starch grains show a wide range of these effects and make finding raw starches so much fun. Try it with your 1st order red for cooler colors.
The effect is due to spherical orientation of crystalline material from a central point. These molecules or microcrystals show extinction positions at the 90, 180, 270 and 360 positions as well as compensation like all birefringent material. Since your polars are at 90 degrees you get a black extinction cross becase there are always some crystals at extriction. The different colors with your 1st order red plate is due because in one direction the crystals add and in the other they subtract. With low order white colors the 1st order red produces two quadrants, as defined by the cross, blue and the other yellow.
I use to find spherlites in degraded polyester, but some organic materials, DDT for one, form colorful spherlites.
Way too much fun with PLM!
Frank
hagglundk1-at-nku.ed u To 04/24/2009 09:40 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] viaWWW: Maltese Cross hagglundk1-at-nku.ed in polarized light microscopy u
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Email: hagglundk1-at-nku.edu Name: Karl Hagglund
Title-Subject: [Filtered] Maltese Cross in polarized light microscopy
Question: I have a customer who has asked about the formation of the maltese cross interference pattern under crossed polarized light. A common example of this can be seen in a variety of starches viewed under crossed polars.
Can someone provide a concise explanation or a good reference that explains why we see the maltese cross?
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Fri Apr 24 08:20:25 2009 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3ODKOSr003559 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:20:25 -0500 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p06240801c6176d07521b-at-[206.69.208.22]} 8, 11 -- Date: Fri, 24 Apr 2009 08:20:23 -0500 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: hagglundk1-at-nku.edu (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: Maltese Cross in polarized light microscopy 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 28, 22 -- From frank_karl-at-lincolnelectric.com Fri Apr 24 09:16:56 2009 28, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OEGti4021198 28, 22 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 09:16:55 -0500 28, 22 -- In-Reply-To: {200904241340.n3ODeNc3008559-at-ns.microscopy.com} 28, 22 -- Subject: Re: [Microscopy] viaWWW: Maltese Cross in polarized light microscopy 28, 22 -- To: hagglundk1-at-nku.edu, Microscopy-at-microscopy.com 28, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 28, 22 -- Message-ID: {OF043FD432.08590E61-ON852575A2.004B3B88-852575A2.004E6550-at-lincolnelectric.com} 28, 22 -- Date: Fri, 24 Apr 2009 10:16:26 -0400 28, 22 -- From: Frank_Karl-at-lincolnelectric.com 28, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 28, 22 -- 07, 2008) at 04/24/2009 10:16:27 AM, 28, 22 -- CD-MIME complete at 04/24/2009 10:16:27 AM, 28, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 28, 22 -- 07, 2008) at 04/24/2009 10:16:27 AM, 28, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 28, 22 -- 07, 2008) at 04/24/2009 10:16:27 AM, 28, 22 -- Serialize complete at 04/24/2009 10:16:27 AM 28, 22 -- MIME-Version: 1.0 28, 22 -- Content-Type: text/plain; 28, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
Every once and a while we run into problems with sections falling off the grids. I know this has been rehashed more than once here, but its hard to find the answers in the archives, so I am asking for the consolidated, final analysis of the problem and solutions.
I suspect it could be students using old, oxidized grids, but I'm not sure enough to just give them that one answer.
I have check on 'grid glues' and searched around, but knew the experts and experienced would be here.
So, what is your strategy. Never had the problem? Clean the grids with solvents or acid, which ones? Grid glue or other solutions?
Lay it on me baby.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009 11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500 11, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 11, 42 -- by localhost (Email Security Appliance) with SMTP id 366EB16B1BB_9F1CEF7B 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:47 +0000 (GMT) 11, 42 -- Received: from sjdccd.cc.ca.us (unknown [207.62.178.236]) 11, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT) 11, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 11, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 Apr 2009 08:04:16 -0700 11, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 11, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 11, 42 -- ESMTP id KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24 11, 42 -- Apr 2009 07:47:04 -0700 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 -0700 (PDT) 11, 42 -- X-Virus-Scanned: amavisd-new at 11, 42 -- X-Spam-Flag: NO 11, 42 -- X-Spam-Score: -2.498 11, 42 -- X-Spam-Level: 11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.000, 11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] 11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 11, 42 -- with ESMTP id 4XiJVc41HOJ8 for {microscopy-at-microscopy.com} ; 11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 (PDT) 11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146]) 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 -0700 (PDT) 11, 42 -- Message-Id: {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu} 11, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 11, 42 -- To: microscopy-at-microscopy.com 11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 11, 42 -- Content-Transfer-Encoding: 7bit 11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 42 -- Subject: Sections falling off grids 11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700 11, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Jonathan, not having had such problems in the long years I'm doing my job here I wonder about the } quality { and kind/type of resin sections you usually are dealing with.
Please could you provide information about wether this is a problem without a relationship to any or this is rellated to a specific resin type you use/ which is used.
Also it would be of interest wether you are talking about "falling off" sections mounted on "naked" copper (standard) or other specific grids (like nickel) or such one prefilmed with/by formvar/butvar/collodion film....
Thanking you and best wishes and regards
Wolfgang MUSS Salzburg, Austria
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} -----Ursprüngliche Nachricht----- } Von: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Gesendet: Freitag, 24. April 2009 17:11 } An: Muß Wolfgang } Betreff: [Microscopy] Sections falling off grids } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } Every once and a while we run into problems with sections falling off the grids. I know this has been rehashed more than once here, but its hard to find the answers in the archives, so I am asking for the consolidated, final analysis of the problem and solutions. } } I suspect it could be students using old, oxidized grids, but I'm not sure enough to just give them that one answer. } } I have check on 'grid glues' and searched around, but knew the experts and experienced would be here. } } So, what is your strategy. Never had the problem? Clean the grids with solvents or acid, which ones? } Grid glue or other solutions? } } Lay it on me baby. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original } Headers============================== } 11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009 } 11, 42 -- Received: from mailin.deltacollege.edu } (mailin.deltacollege.edu [207.62.178.150]) } 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id n3OF4QiE012517 } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr } 2009 10:04:44 -0500 } 11, 42 -- Received: from mailin.deltacollege.edu } (localhost.localdomain [127.0.0.1]) } 11, 42 -- by localhost (Email Security Appliance) with
I have had this trouble in the past but not for a long time since I bagan flaming the grids. I saw this in the Bozzola and Russell book. Works better for standard grids; be very careful with thin bar grids. Use an alcohol burner with a small flame (~1cm max). Pick up a grid with forceps and sweep briefly through the tip of the flame. It is better to do too little than too much since it can be repeated until the desired effect is acheived; after a bit you get it right almost always in one or 2 passes. What you are looking for is a "scorched" look, some interference colors in the red and blue range (which must be thicknesses of surface modification (oxidation?). These grids wet beautifully and sections cling tenaciously. For the record, I always pick up sections on the shiny side; I know there are 2 teams on this topic :-) My logic is that it is like kitchen plastic film that clings better to smooth surfaces....
I have had wettability issues with gold and gided grids that can't be flamed. For these I treat 15 sec in the Harrick Plasma cleaner and they wet beautifully and sections adhere well. This probably works for the copper grids as well but I haven't tried.
Cheers!
Dale
jkrupp-at-deltacollege.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } Every once and a while we run into problems with sections falling off } the grids. I know this has been rehashed more than once here, but its } hard to find the answers in the archives, so I am asking for the } consolidated, final analysis of the problem and solutions. } } I suspect it could be students using old, oxidized grids, but I'm not } sure enough to just give them that one answer. } } I have check on 'grid glues' and searched around, but knew the experts } and experienced would be here. } } So, what is your strategy. Never had the problem? Clean the grids } with solvents or acid, which ones? Grid glue or other solutions? } } Lay it on me baby. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original Headers============================== } 11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009 } 11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517 } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500 } 11, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 11, 42 -- by localhost (Email Security Appliance) with SMTP id 366EB16B1BB_9F1CEF7B } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:47 +0000 (GMT) } 11, 42 -- Received: from sjdccd.cc.ca.us (unknown [207.62.178.236]) } 11, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT) } 11, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) } 11, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) } 11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 Apr 2009 08:04:16 -0700 } 11, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by } 11, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 11, 42 -- ESMTP id KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24 } 11, 42 -- Apr 2009 07:47:04 -0700 } 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054 } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 -0700 (PDT) } 11, 42 -- X-Virus-Scanned: amavisd-new at } 11, 42 -- X-Spam-Flag: NO } 11, 42 -- X-Spam-Score: -2.498 } 11, 42 -- X-Spam-Level: } 11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.000, } 11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] } 11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 11, 42 -- with ESMTP id 4XiJVc41HOJ8 for {microscopy-at-microscopy.com} ; } 11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 (PDT) } 11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146]) } 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040 } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 -0700 (PDT) } 11, 42 -- Message-Id: {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu} } 11, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 11, 42 -- To: microscopy-at-microscopy.com } 11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 11, 42 -- Content-Transfer-Encoding: 7bit } 11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 11, 42 -- Subject: Sections falling off grids } 11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700 } 11, 42 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - Headers==============================
Hi Jon, I routinely use a 'grid glue' as I like to immunolabel my sections immersed in the immunoreagents as it makes the whole labelling process easier and enhances the labelling, with the antibody/antibodies having access to epitopes on both section surfaces. This latter means there is a tendency to lose sections when transferring grids from one solution to the next. Anyway my trick is to just briefly immerse the grids in c.5ml of chloroform in which about 4-5" of sellotape has been dissolved (remove the tape itself once the glue has dissolved off it - just shake for a few moments). This seems to work for me and hope it does for you! Cheers, Jules
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
--On 24 April 2009 10:18 -0500 jkrupp-at-deltacollege.edu wrote:
} } } } ------------------------------------------------------------------------- } --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------- } --- } } Hi: } } Every once and a while we run into problems with sections falling off } the grids. I know this has been rehashed more than once here, but its } hard to find the answers in the archives, so I am asking for the } consolidated, final analysis of the problem and solutions. } } I suspect it could be students using old, oxidized grids, but I'm not } sure enough to just give them that one answer. } } I have check on 'grid glues' and searched around, but knew the experts } and experienced would be here. } } So, what is your strategy. Never had the problem? Clean the grids } with solvents or acid, which ones? Grid glue or other solutions? } } Lay it on me baby. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } ==============================Original } Headers============================== 11, 42 -- From } jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009 } 11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu } [207.62.178.150]) 11, 42 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517 11, 42 -- for } {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500 11, 42 -- } Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 11, 42 -- by localhost (Email Security Appliance) with SMTP id } 366EB16B1BB_9F1CEF7B 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 } Apr 2009 14:38:47 +0000 (GMT) 11, 42 -- Received: from sjdccd.cc.ca.us } (unknown [207.62.178.236]) 11, 42 -- by mailin.deltacollege.edu (Sophos } Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F 11, 42 -- for } {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT) 11, 42 } -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 11, 42 } -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) } 11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 } Apr 2009 08:04:16 -0700 11, 42 -- Received: from zmail.deltacollege.edu } ([207.62.178.179]) by 11, 42 -- sunspot.sjdccd.cc.ca.us } (Netscape Messaging Server 4.15) with 11, 42 -- ESMTP id } KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24 11, 42 -- } Apr 2009 07:47:04 -0700 } 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054 } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 } -0700 (PDT) 11, 42 -- X-Virus-Scanned: amavisd-new at } 11, 42 -- X-Spam-Flag: NO } 11, 42 -- X-Spam-Score: -2.498 } 11, 42 -- X-Spam-Level: } 11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 } tests=[AWL=0.000, 11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] } 11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) } (amavisd-new, port 10024) 11, 42 -- with ESMTP id 4XiJVc41HOJ8 for } {microscopy-at-microscopy.com} ; 11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 } (PDT) } 11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146]) } 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040 } 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 } -0700 (PDT) 11, 42 -- Message-Id: } {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu} 11, 42 -- From: } Jon Krupp {jkrupp-at-deltacollege.edu} } 11, 42 -- To: microscopy-at-microscopy.com } 11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes 11, 42 -- Content-Transfer-Encoding: 7bit } 11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) } 11, 42 -- Subject: Sections falling off grids } 11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700 } 11, 42 -- X-Mailer: Apple Mail (2.930.3) } ==============================End of - } Headers==============================
Epon thin sections coming off naked grids during staining procedures was solved when I followed Debby Sherman¹s suggestion of putting the grids containing sections into my oven for at least 15 min. to overnight before staining. I only need to do this occasionally since I do not have trouble frequently (do not know why). I only use my oven for this treatment when it is empty because I do not know if the fumes that are generated by curing epoxy would have an effect on the grids even though they are either in a closed Petri Dish on filter paper or in a Grid Box.
My newer grids (Electron Microscopy Sciences) do not need much cleaning but old ones do. If you sonicate in Acetone to remove oil/grease residues I found it advisable to do a final rinse in 100% ethanol before drying since I think the Acetone sometimes leaves something behind on the grid surface. This reduced the number of sections that I lost.
I have also used a very careful QUICK dip in HNO3 followed by several water washes to make naked grids hydrophilic and hence make section pick-up from the boat much easier. I had good luck with sections sticking where they belonged with this technique. The problem was using the strong acid which dissolved the grid if it was not washed quickly enough.
Wishing you good grids, Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
==================== X-from: {W.Muss-at-salk.at}
Good morning/good afternoon, Hi all,
Jonathan, not having had such problems in the long years I'm doing my job here I wonder about the } quality { and kind/type of resin sections you usually are dealing with.
Please could you provide information about wether this is a problem without a relationship to any or this is rellated to a specific resin type you use/ which is used.
Also it would be of interest wether you are talking about "falling off" sections mounted on "naked" copper (standard) or other specific grids (like nickel) or such one prefilmed with/by formvar/butvar/collodion film....
Thanking you and best wishes and regards
Wolfgang MUSS Salzburg, Austria
=========================================================== This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal. ==========================================================
} -----Ursprüngliche Nachricht----- } Von: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Gesendet: Freitag, 24. April 2009 17:11 } An: Muß Wolfgang } Betreff: [Microscopy] Sections falling off grids } } Hi: } } Every once and a while we run into problems with sections falling off the grids. I know this has been rehashed more than once here, but its hard to find the answers in the archives, so I am asking for the consolidated, final analysis of the problem and solutions. } } I suspect it could be students using old, oxidized grids, but I'm not sure enough to just give them that one answer. } } I have check on 'grid glues' and searched around, but knew the experts and experienced would be here. } } So, what is your strategy. Never had the problem? Clean the grids with solvents or acid, which ones? } Grid glue or other solutions? } } Lay it on me baby. } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu
==============================Original Headers============================== 18, 32 -- From connellyps-at-nhlbi.nih.gov Fri Apr 24 11:14:09 2009 18, 32 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OGE8g2006300 18, 32 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 11:14:09 -0500 18, 32 -- X-IronPortListener: Outbound_SMTP 18, 32 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) 18, 32 -- by nihxway5out.hub.nih.gov with ESMTP; 24 Apr 2009 12:13:48 -0400 18, 32 -- Received: from NIHHT01.nih.gov ([156.40.71.20]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); 18, 32 -- Fri, 24 Apr 2009 12:13:48 -0400 18, 32 -- Received: from NIHHTRC.nih.gov (156.40.71.74) by NIHHT01.nih.gov 18, 32 -- (156.40.71.20) with Microsoft SMTP Server (TLS) id 8.1.340.0; Fri, 24 Apr 18, 32 -- 2009 12:13:47 -0400 18, 32 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHTRC.nih.gov 18, 32 -- ([156.40.71.74]) with mapi; Fri, 24 Apr 2009 12:13:47 -0400 18, 32 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 18, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 18, 32 -- Date: Fri, 24 Apr 2009 12:12:03 -0400 18, 32 -- Subject: Re: [Microscopy] Re: Sections falling off grids 18, 32 -- Thread-Topic: [Microscopy] Re: Sections falling off grids 18, 32 -- Thread-Index: AcnE8eZu1Uhb/0O4QnSLGJa4eYH8nQABYEe8 18, 32 -- Message-ID: {C6175D13.34E9%connellyps-at-nhlbi.nih.gov} 18, 32 -- In-Reply-To: {200904241532.n3OFW3k3002378-at-ns.microscopy.com} 18, 32 -- Accept-Language: en, en-US 18, 32 -- Content-Language: en 18, 32 -- X-MS-Has-Attach: 18, 32 -- X-MS-TNEF-Correlator: 18, 32 -- acceptlanguage: en, en-US 18, 32 -- Content-Type: text/plain; charset="iso-8859-1" 18, 32 -- MIME-Version: 1.0 18, 32 -- X-OriginalArrivalTime: 24 Apr 2009 16:13:48.0309 (UTC) FILETIME=[A6516C50:01C9C4F7] 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OGE8g2006300 ==============================End of - Headers==============================
Jonathan, I agree that we should know more details, like if this is routine counterstaining or IHC. For basic counterstaining, a simple acid wash with proper rinsing and drying should be fine.
I have experienced highly variable section adhesion when I perform IHC with any Tween or Triton detergents, primarily when the grids sink into the reagent droplets (using square pattern, thin bar nickel grids). If this is your case, then I sympathize.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Friday, April 24, 2009 11:18 AM To: Sobocinski, Gregg
Hi:
Every once and a while we run into problems with sections falling off the grids. I know this has been rehashed more than once here, but its hard to find the answers in the archives, so I am asking for the consolidated, final analysis of the problem and solutions.
I suspect it could be students using old, oxidized grids, but I'm not sure enough to just give them that one answer.
I have check on 'grid glues' and searched around, but knew the experts and experienced would be here.
So, what is your strategy. Never had the problem? Clean the grids with solvents or acid, which ones? Grid glue or other solutions?
Lay it on me baby.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 11, 42 -- From jkrupp-at-deltacollege.edu Fri Apr 24 10:04:45 2009 11, 42 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3OF4QiE012517 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 10:04:44 -0500 11, 42 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 11, 42 -- by localhost (Email Security Appliance) with SMTP id 366EB16B1BB_9F1CEF7B 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:47 +0000 (GMT) 11, 42 -- Received: from sjdccd.cc.ca.us (unknown [207.62.178.236]) 11, 42 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id A691E16AAD9_9F1CEF5F 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:38:45 +0000 (GMT) 11, 42 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 11, 42 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 11, 42 -- with ESMTP id 46818056 for microscopy-at-microscopy.com; Fri, 24 Apr 2009 08:04:16 -0700 11, 42 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 11, 42 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 11, 42 -- ESMTP id KIM12G00.7HY for {microscopy-at-microscopy.com} ; Fri, 24 11, 42 -- Apr 2009 07:47:04 -0700 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D6C1A3A55054 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:15 -0700 (PDT) 11, 42 -- X-Virus-Scanned: amavisd-new at 11, 42 -- X-Spam-Flag: NO 11, 42 -- X-Spam-Score: -2.498 11, 42 -- X-Spam-Level: 11, 42 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.000, 11, 42 -- BAYES_00=-2.599, RDNS_NONE=0.1] 11, 42 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 11, 42 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 11, 42 -- with ESMTP id 4XiJVc41HOJ8 for {microscopy-at-microscopy.com} ; 11, 42 -- Fri, 24 Apr 2009 08:04:14 -0700 (PDT) 11, 42 -- Received: from [172.20.2.146] (unknown [172.20.2.146]) 11, 42 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 6B8233A55040 11, 42 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 08:04:14 -0700 (PDT) 11, 42 -- Message-Id: {8789DA5B-6ECE-4DB6-96D3-607A25EA140A-at-deltacollege.edu} 11, 42 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 11, 42 -- To: microscopy-at-microscopy.com 11, 42 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 11, 42 -- Content-Transfer-Encoding: 7bit 11, 42 -- Mime-Version: 1.0 (Apple Message framework v930.3) 11, 42 -- Subject: Sections falling off grids 11, 42 -- Date: Fri, 24 Apr 2009 08:04:13 -0700 11, 42 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
File this Tip of the Week under "Okay, so why didn't I think of this before, dummy?"
We have one of those hand-held carbon rod sharpeners that require you to twist the rod manually in order to get the narrow tip needed for evaporating. They are hard and uncomfortable to use, often break the tip off just as you're finally getting it to the length you need, and just generally a pain. But I could never bring myself to spring for the hundreds of bucks for a decent sharpener, especially considering the volume of evaporating we do.
As I was cranking away this morning, breaking tips and saying bad words, I remembered that we had a Dremel tool in the next room. Took that carbon rod, put it in the drill bit chuck, turned the tool on at its lowest speed, inserted the rod into the sharpener and had my tip in about 17 seconds.
Many of you probably had this one figured out a while ago, but if not, here it is.
I now have the will to go on evaporating.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 10, 27 -- From TindallR-at-missouri.edu Fri Apr 24 11:23:07 2009 10, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OGN64h021629 10, 27 -- for {microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 11:23:07 -0500 10, 27 -- X-IronPort-Anti-Spam-Filtered: true 10, 27 -- X-IronPort-Anti-Spam-Result: ApoEAE6E8UnRauUo/2dsb2JhbADAIAEJhz+ITIJBgTMF 10, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 10, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 24 Apr 2009 11:23:06 -0500 10, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 27 -- Fri, 24 Apr 2009 11:23:06 -0500 10, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 27 -- Content-class: urn:content-classes:message 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; 10, 27 -- charset="us-ascii" 10, 27 -- Subject: Sharpening carbon rods without saying bad words 10, 27 -- Date: Fri, 24 Apr 2009 11:22:35 -0500 10, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD808D-at-UM-XMAIL08.um.umsystem.edu} 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- Thread-Topic: Sharpening carbon rods without saying bad words 10, 27 -- Thread-Index: AcnE+OAKZMl5kF/XSr6NbxcPDeYSrg== 10, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 27 -- To: {microscopy-at-microscopy.com} 10, 27 -- X-OriginalArrivalTime: 24 Apr 2009 16:23:06.0543 (UTC) FILETIME=[F30D2BF0:01C9C4F8] 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OGN64h021629 ==============================End of - Headers==============================
One thing missing from this thread is an explicit discussion of the technique for picking up sections. I have always picked up sections from below, and after 25 years and thousands of blocks, I have never had a problem with sections falling off bare copper grids without adhesive, either dull or shiny side, with Epon, Spurr's, or LR White. When you bring the grid up from below, there is water between the section and the grid, and when the water evaporates, the section becomes bonded tightly to the grid. This is not so when you come from above.
I should add that I sonicate the grids in 100% ethanol then place them on filter paper in a petri dish. If you have a problem with the sections running away when you try to pick up sections, try dipping the grid briefly in ethanol, then immediately rinse it thoroughly in distilled water, and bring the wet grid to the boat. The purpose of this is not to clean the grid, but to avoid the formation of tiny air bubbles on the grid, which tend to repel the sections. You should also rotate the grid as you remove it from the boat, so that it is vertical when it comes out of the water. This avoids bringing up a large drop of water with the grid, and having your sections shift when the water is blotted off.
Ralph Common Michigan State Univ.
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Fri Apr 24 12:24:42 2009 4, 24 -- Received: from sys50.mail.msu.edu (sys50.mail.msu.edu [35.9.75.230]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OHOf4L017349 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:24:42 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys50.mail.msu.edu with esmtpsa (Exim 4.63 #12) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1LxP8i-0000e5-IZ 4, 24 -- for Microscopy-at-microscopy.com; Fri, 24 Apr 2009 13:24:40 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Sections falling off grids 4, 24 -- Date: Fri, 24 Apr 2009 14:24:19 -0400 4, 24 -- Message-ID: {004101c9c509$e2c775f0$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- Importance: Normal 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
} I have also used a very careful QUICK dip in HNO3 followed } by several water washes to make naked grids hydrophilic and } hence make section pick-up from the boat much easier. I had } good luck with sections sticking where they belonged with } this technique. The problem was using the strong acid which } dissolved the grid if it was not washed quickly enough. } } Wishing you good grids, } Pat
You do not need to use strong acid. 1N HCl can do job just fine. You can keep a grid in this acid for a while without problems.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Hi Jon, I, too, was plagued with sections falling off grids. Since I have been following the following, I have not lost a single section! Dip copper grid into a 0.1N solution of HCl (I usually count 10 seconds with a drop of HCL on the grid) and blot dry. Dip several times into 100% acetone to rinse and allow to dry on filter paper. I usually prepare the grids before I start sectioning so I don't have to stop each time to get my grids ready. Once my sections are collected, I put the grids into the oven for 20 minutes to dry. I can stain immediately after removing the sections from the oven or wait until later, it doesn't seem to matter. Hope this helps your students! There is nothing worse than spending your day sectioning and seeing only shreds on the TEM! Take care, Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {connellyps-at-nhlbi.nih.gov} To: {pekysar-at-ucdavis.edu} Sent: Friday, April 24, 2009 9:20 AM
Hello. We have traditionally been a Life Sciences Imaging core facility on campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding ourselves leaning more towards the Material Sciences. As such, we would like to augment our Center's reference library with books relating to the imaging and analytical side of microscopy
I was wondering if I could get ideas on basic book requirements that Material scientists would look at as basic references and might be considered indispensable in a Materials oriented research center. Thanks for any help! Mark
Mark Grimson, PhD Manager, The Imaging Center c/o The Department of Biological Sciences Flint and Main Texas Tech University Lubbock, TX 79409-3131
You can try something other than water to fill knife boat. I used ethylene glycol for cutting mineralized cell cultures with some success. It is toxic, easily wet block face, and evaporates much slower than water (I kept grids overnight to let them dry out). I have not idea about solubility of P4VP in ethylene glycol. By the way, have you checked you grids with TEM? Sometimes, when floating in a boat, embedded specimen could be transparent (invisible), while resin around it had usual silver or gold color.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: leolukman-at-wisc.edu [mailto:leolukman-at-wisc.edu] } Sent: Thursday, April 23, 2009 1:22 PM } To: Dusevich, Vladimir } Subject: [Microscopy] sectioning p4vp } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Hi everyone, } } I'm a graduate student at Materials Science Program. I tried } to section a poly(4-vinylpyridine) (P4VP) embedded in epon } with a diamond knife and water on the boat. However, it } seemed that the water ate away my sample and the knife only } cut the epon. If i looked at the sample face after trying to } section, i could see that the sample face had become rough, } which i assumed because the water somehow dissolving the } sample (In large amount, P4VP is insoluble in water). Would } you please suggest me how to resolve this problem? Thank you } very much. } } Sincerely, } Melvina Leolukman } } ==============================Original } Headers============================== } 3, 33 -- From leolukman-at-wisc.edu Thu Apr 23 13:21:09 2009 3, } 33 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu } [144.92.197.210]) } 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n3NIL85O027207 } 3, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Apr } 2009 13:21:08 -0500 } 3, 33 -- MIME-version: 1.0 } 3, 33 -- Content-transfer-encoding: 7BIT 3, 33 -- } Content-disposition: inline 3, 33 -- Content-type: } text/plain; charset=us-ascii 3, 33 -- Received: from } avs-daemon.smtpauth1.wiscmail.wisc.edu by 3, 33 -- } smtpauth1.wiscmail.wisc.edu 3, 33 -- (Sun Java(tm) System } Messaging Server 7.0-5.01 32bit (built Feb 19 2009)) 3, 33 -- } id {0KIK00E02GB8KN00-at-smtpauth1.wiscmail.wisc.edu} for 3, 33 } -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 13:21:08 } -0500 (CDT) 3, 33 -- Received: from wiscmail.wisc.edu } (store1.doit.wisc.edu [144.92.8.164]) 3, 33 -- by } smtpauth1.wiscmail.wisc.edu 3, 33 -- (Sun Java(tm) System } Messaging Server 7.0-5.01 32bit (built Feb 19 2009)) 3, 33 -- } with ESMTP id {0KIK009W9GB76120-at-smtpauth1.wiscmail.wisc.edu} } for 3, 33 -- Microscopy-at-microscopy.com; Thu, 23 Apr 2009 } 13:21:07 -0500 (CDT) 3, 33 -- Received: from [144.92.8.222] } (Forwarded-For: 128.104.185.23, [144.92.197.247]) 3, 33 -- } by store1.doit.wisc.edu (mshttpd); Thu, 23 Apr 2009 13:21:07 } -0500 3, 33 -- Date: Thu, 23 Apr 2009 13:21:07 -0500 3, 33 -- } From: MELVINA LEOLUKMAN {leolukman-at-wisc.edu} 3, 33 -- } Subject: sectioning p4vp 3, 33 -- To: } Microscopy-at-microscopy.com 3, 33 -- Message-id: } {e51c89d31f6ec.49f06b43-at-wiscmail.wisc.edu} } 3, 33 -- X-Mailer: Sun Java(tm) System Messenger Express } 6.2-8.04 (built Feb 28 2007) 3, 33 -- Content-language: en 3, } 33 -- X-Accept-Language: en 3, 33 -- Priority: normal 3, 33 } -- X-Spam-Report: TrustedSender=yes, SenderIP=144.92.8.164 3, } 33 -- X-Spam-PmxInfo: Server=avs-10, Version=5.5.1.360522, 3, } 33 -- Antispam-Engine: 2.6.1.350677, Antispam-Data: } 2009.4.23.180432, 3, 33 -- SenderIP=144.92.8.164 } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 25 -- From DusevichV-at-umkc.edu Fri Apr 24 12:54:45 2009 7, 25 -- Received: from kc-msxproto1.kc.umkc.edu (exchange.umkc.edu [134.193.143.167]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OHshSW028893 7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 12:54:44 -0500 7, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 25 -- Fri, 24 Apr 2009 12:54:42 -0500 7, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 25 -- Content-class: urn:content-classes:message 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; 7, 25 -- charset="US-ASCII" 7, 25 -- Subject: RE: [Microscopy] sectioning p4vp 7, 25 -- Date: Fri, 24 Apr 2009 12:54:41 -0500 7, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB838-at-KC-MSX1.kc.umkc.edu} 7, 25 -- In-Reply-To: {200904231822.n3NIMSL3028113-at-ns.microscopy.com} 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- Thread-Topic: [Microscopy] sectioning p4vp 7, 25 -- thread-index: AcnEQHeCBGxAvokiRn6Wcw1dqRRKbQAwzd0Q 7, 25 -- References: {200904231822.n3NIMSL3028113-at-ns.microscopy.com} 7, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 25 -- To: {leolukman-at-wisc.edu} , {Microscopy-at-microscopy.com} 7, 25 -- X-OriginalArrivalTime: 24 Apr 2009 17:54:42.0908 (UTC) FILETIME=[BF23E9C0:01C9C505] 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3OHshSW028893 ==============================End of - Headers==============================
Polymer microscopy is my life. I would recommend the most recent edition of Polymer Microscopy by Linda C. Sawyer and David T. Grubb. You probably already have Scanning Electron Microscopy and X-Ray Microanalysis by Goldstein, Newbury, Joy et. al. It is not solely about materials but does cover them and I believe an indispensable book.
Regards, Jackie
Jacqueline Ayotte Microscopist - Advanced Materials Characterization Ticona 8040 Dixie Highway } Florence KY 41042 } 859-372-3139 } fax 859-372-3184 } jacqueline.ayotte-at-ticona.com The information contained in this e-mail, and any attachments thereto, is confidential and is intended only for use by the individual(s) and/or entity named above. If you are not the intended recipient of this e-mail, you are hereby notified that any dissemination, distribution or copying of this communication or any disclosure of the contents of this communication to others is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this e-mail. Please then delete the original including all attachments and any copy of any e-mail and printout thereof.
-----Original Message----- X-from: mark.grimson-at-ttu.edu [mailto:mark.grimson-at-ttu.edu] Sent: Friday, April 24, 2009 2:03 PM To: Ayotte, Jacqueline M., Ticona/US
Hello. We have traditionally been a Life Sciences Imaging core facility on campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding ourselves leaning more towards the Material Sciences. As such, we would like to augment our Center's reference library with books relating to the imaging and analytical side of microscopy
I was wondering if I could get ideas on basic book requirements that Material scientists would look at as basic references and might be considered indispensable in a Materials oriented research center. Thanks for any help! Mark
Mark Grimson, PhD Manager, The Imaging Center c/o The Department of Biological Sciences Flint and Main Texas Tech University Lubbock, TX 79409-3131
You should start with some books from ASM (American Society of Materials). Two basic books would be Volume 9 of the ASM Handbook, Metallography and Microstructures (LM of Metals) and Volume 11 of the ASM Handbook Failure Analysis and Prevention (SEM). It depends on how extensive you want to be in your library. There are many other good books, but those are two good books to start with.
Gerald Shulke Materials Engineering Specialist Chrysler LLC
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Hello. We have traditionally been a Life Sciences Imaging core facility on campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding ourselves leaning more towards the Material Sciences. As such, we would like to augment our Center's reference library with books relating to the imaging and analytical side of microscopy
I was wondering if I could get ideas on basic book requirements that Material scientists would look at as basic references and might be considered indispensable in a Materials oriented research center. Thanks for any help! Mark
Mark Grimson, PhD Manager, The Imaging Center c/o The Department of Biological Sciences Flint and Main Texas Tech University Lubbock, TX 79409-3131
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OJ7JD3022539 for {MicroscopyListserverArchive-at-microscopy.com} ; Fri, 24 Apr 2009 14:07:19 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id n3OJ7JRN022537; Fri, 24 Apr 2009 14:07:19 -0500
Hi Mark,
TEM: Willilams & Carter, "Transmission Electron Microscopy" New editioin due this June Reimer & Kohl, "Transmission Electron Microscopy: Physics of Image Formation (Springer Series in Optical Sciences)" SEM: Goldstein et al., "Scanning Electron Microscopy and X-Ray Microanalysis" get the latest edition -- 3rd, I think Reimer, "Scanning Electron Microscopy: Physics of Image Formation and Microanalysis (Springer Series in Optical Sciences" 1998, though. General: Sawyer & Grubb, "Polymer Microscopy" 3rd edition For a start. What I have a hard time finding is a reference on specimen preparation for materials science. Be interesting to see if anyone posts such a book.
Phil
} Hello. We have traditionally been a Life Sciences Imaging core facility on } campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding } ourselves leaning more towards the Material Sciences. As such, we would } like to augment our Center's reference library with books relating to the } imaging and analytical side of microscopy } } I was wondering if I could get ideas on basic book requirements that } Material scientists would look at as basic references and might be } considered indispensable in a Materials oriented research center. Thanks for } any help! Mark } } } } Mark Grimson, PhD } Manager, The Imaging Center } c/o The Department of Biological Sciences } Flint and Main } Texas Tech University } Lubbock, TX 79409-3131 } } mark.grimson-at-ttu.edu } 806-742-3722 x235 (Office) } 806-252-3879 (Cell) } 806-742-2963 (FAX) -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 23 -- From oshel1pe-at-cmich.edu Fri Apr 24 14:07:18 2009 4, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OJ7IKc022534 4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:07:18 -0500 4, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 23 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n3OJ7Ivo011624 4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:07:18 -0400 4, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 23 -- Fri, 24 Apr 2009 15:07:17 -0400 4, 23 -- Mime-Version: 1.0 4, 23 -- Message-Id: {f06240816c617be48e46a-at-[141.209.160.249]} 4, 23 -- Date: Fri, 24 Apr 2009 15:07:11 -0400 4, 23 -- To: Microscopy-at-microscopy.com 4, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 23 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas 4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 23 -- X-OriginalArrivalTime: 24 Apr 2009 19:07:18.0060 (UTC) FILETIME=[E3035EC0:01C9C50F] 4, 23 -- X-Canit-CHI2: 0.00 4, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 23 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 23 -- X-CanItPRO-Stream: default 4, 23 -- X-Canit-Stats-ID: 12386593 - 56aaaf500e5c 4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
My technique for cleaning grids is a variation on Pat's method, by using acetone and HCl together.
I mix up a 250 ml lot of cleaning solution as follows: 25 ml conc. HCl, 175 ml distilled water, 50 ml acetone(99%). Of course, add the acid to the water, then add the acetone.
I sonicate copper or nickel grids, mesh or slots, for about 30 seconds in a 25 ml beaker with about 10 ml solution. Then I pour that off, and sonicate once with 99% acetone as a rinse. Then invert beaker onto clean filter paper to air dry. The grids usually stick a bit to inside of beaker but will fall off when they dry.
I do this each day before I begin sectioning. The copper grids get so clean - fresh copper exposed - that they may oxidize enough over night to need cleaning again even just a day later. I also clean grids this way before any coating with films, like Formvar or Butvar.
This method of cleaning has worked for me for many years and sections stick to the grid.
Oh, I also pick up floating sections from above onto the dull side of the grids. Others report good results picking up from below the floating sections, or onto the shiny side of the grids. Others report good results cleaning grids by quickly flaming them in an alcohol lamp flame.
In this new age of solar energy, I have not yet tried cleaning grids by concentrating the suns rays with a 4" hand lens onto grids resting on a clean, refractory surface. But if it worked, that would be soooo green! Someone ought to try this.
Hey - whatever works babe, like, whatever!!
Gib Ahlstrand Imaging Center University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://cbs.umn.edu/ic/
This message sent from my Mac Power PC G4 400 MHz beast! Grrrrr! ---------------------------------
-------- Original Message --------
Hi Jon, I, too, was plagued with sections falling off grids. Since I have been following the following, I have not lost a single section! Dip copper grid into a 0.1N solution of HCl (I usually count 10 seconds with a drop of HCL on the grid) and blot dry. Dip several times into 100% acetone to rinse and allow to dry on filter paper. I usually prepare the grids before I start sectioning so I don't have to stop each time to get my grids ready. Once my sections are collected, I put the grids into the oven for 20 minutes to dry. I can stain immediately after removing the sections from the oven or wait until later, it doesn't seem to matter. Hope this helps your students! There is nothing worse than spending your day sectioning and seeing only shreds on the TEM! Take care, Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {connellyps-at-nhlbi.nih.gov} To: {pekysar-at-ucdavis.edu} Sent: Friday, April 24, 2009 9:20 AM
Gerald,
I'm looking for a general (" ") sample prep book that discusses the "how to", "why to", and wherefores of the various methods -- ion-milling, etching, tripod polishing, small-angle cleavage, etc. -- for a variety of different kinds of materials. Naturally, all this varies with the sample type and microscopy, as well as with the questions asked.
So, not a specific, say, fractography book, or thin-film book, or even more-general-but-still-specific book such as for metallurgy, but a text like one would use in a materials EM course, but concentrated on sample prep, as opposed to a chapter on sample prep in a more general EM text. Which would also be handy to have hanging around the facility when whoever walks in with whatever kind of sample they have. Like we have in biology.
Phil
Phil,
Sample prep, well, I can say it depends....
What type of samples are you interested in? Metals, plastics, or ceramics? Are you more interested in light microscopy or electron microscopy?
Basically we use microscopy for two things: materials characterization and failure analysis. For materials characterization there are a number of protocols depending on the material and what you are looking for in that material. For metals, let's say, it depends on the alloy, how it was processed, and what phases you are looking for. Most sample are mounted in Bakelite or epoxy, ground, polished, and etched to reveal the microstructure. For failure analysis, there is not much sample prep. Usually you are looking for contaminants, deposits, etc. so you look at the sample in the as received condition first. After you have collected what information you can, then you clean the sample to see the fracture surface topography. For metals, it may be acetone or hexanes to remove oil and dirt. There are more aggressive approaches, but the idea is to remove most of the surface contamination without damaging the underlying material. It depends on if the sample was corroded or not. For polymers, the surface may be cleaned with soapy water. Anything harsher can destroy the surface. If the material is not very conductive for SEM, then we gold sputter coat the sample. Usually this is a last resort, because you can't take it back off. You can do a lot with low voltage or in an ESEM.
I can go into more detail if you want. I can post to the server to share with everyone if you could tell me more specifically what you are after. There are numerous books, especially for metals.
Gerald Shulke Materials Engineering Specialist Chrysler LLC
Hi Mark,
TEM: Willilams & Carter, "Transmission Electron Microscopy" New editioin due this June Reimer & Kohl, "Transmission Electron Microscopy: Physics of Image Formation (Springer Series in Optical Sciences)" SEM: Goldstein et al., "Scanning Electron Microscopy and X-Ray Microanalysis" get the latest edition -- 3rd, I think Reimer, "Scanning Electron Microscopy: Physics of Image Formation and Microanalysis (Springer Series in Optical Sciences" 1998, though. General: Sawyer & Grubb, "Polymer Microscopy" 3rd edition For a start. What I have a hard time finding is a reference on specimen preparation for materials science. Be interesting to see if anyone posts such a book.
Phil
} Hello. We have traditionally been a Life Sciences Imaging core facility on } campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding } ourselves leaning more towards the Material Sciences. As such, we would } like to augment our Center's reference library with books relating to the } imaging and analytical side of microscopy } } I was wondering if I could get ideas on basic book requirements that } Material scientists would look at as basic references and might be } considered indispensable in a Materials oriented research center. Thanks for } any help! Mark } } } } Mark Grimson, PhD } Manager, The Imaging Center } c/o The Department of Biological Sciences } Flint and Main } Texas Tech University } Lubbock, TX 79409-3131 } } mark.grimson-at-ttu.edu } 806-742-3722 x235 (Office) } 806-252-3879 (Cell) } 806-742-2963 (FAX) -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Our high voltage cable went bad on our little old Zeiss EM10, vintage 1976. Would anyone have a spare? Can you recommend a company to make a new one? Thanks,
Roseann Csencsits, PhD Scientist in Charge - Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
==============================Original Headers============================== 2, 23 -- From RCsencsits-at-lbl.gov Fri Apr 24 15:25:11 2009 2, 23 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OKPBAX028666 2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:25:11 -0500 2, 23 -- X-Ironport-SBRS: 2.3 2, 23 -- X-IronPort-Anti-Spam-Filtered: true 2, 23 -- X-IronPort-Anti-Spam-Result: Aj4CAIO88UmAAykYe2dsb2JhbACWTgEBFiIFqUkJj3aCQYEzBQ 2, 23 -- X-IronPort-AV: E=Sophos;i="4.40,243,1239001200"; 2, 23 -- d="scan'208";a="12586616" 2, 23 -- Received: from mta1.lbl.gov ([128.3.41.24]) 2, 23 -- by ironport4.lbl.gov with ESMTP; 24 Apr 2009 13:25:10 -0700 2, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246]) 2, 23 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n3OKPADO025737 2, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Apr 2009 13:25:10 -0700 (PDT) 2, 23 -- Message-Id: {EF4BAEF5-F5E7-49C1-B756-C1AB159127BF-at-lbl.gov} 2, 23 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 23 -- Content-Transfer-Encoding: 7bit 2, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 2, 23 -- Subject: HT cable wanted for Zeiss EM10 2, 23 -- Date: Fri, 24 Apr 2009 13:25:10 -0700 2, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
I'll refer to my other post for good general books for material science for both LM and SEM. I do not know of a specific book for sample prep for SEM in the materials field. There is an older book "SEM: A User's Manual for Material Science" by Gabriel, also published by ASM, but it is from the 80's and somewhat outdated. Sample prep techniques for SEM is usually covered in a chapter or a general discussion in several books based on failure analysis or specific for a type of material. For general sample prep of metals for LM, I would add "Metallography Principles and Practice" by George Vander Voort.
Gerald Shulke Materials Specialist Chrysler LLC
-----oshel1pe-at-cmich.edu wrote: -----
To: gas19-at-chrysler.com X-from: oshel1pe-at-cmich.edu
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Gerald,
I'm looking for a general (" ") sample prep book that discusses the "how to", "why to", and wherefores of the various methods -- ion-milling, etching, tripod polishing, small-angle cleavage, etc. -- for a variety of different kinds of materials. Naturally, all this varies with the sample type and microscopy, as well as with the questions asked.
So, not a specific, say, fractography book, or thin-film book, or even more-general-but-still-specific book such as for metallurgy, but a text like one would use in a materials EM course, but concentrated on sample prep, as opposed to a chapter on sample prep in a more general EM text. Which would also be handy to have hanging around the facility when whoever walks in with whatever kind of sample they have. Like we have in biology.
Phil
Phil,
Sample prep, well, I can say it depends....
What type of samples are you interested in? Metals, plastics, or ceramics? Are you more interested in light microscopy or electron microscopy?
Basically we use microscopy for two things: materials characterization and failure analysis. For materials characterization there are a number of protocols depending on the material and what you are looking for in that material. For metals, let's say, it depends on the alloy, how it was processed, and what phases you are looking for. Most sample are mounted in Bakelite or epoxy, ground, polished, and etched to reveal the microstructure. For failure analysis, there is not much sample prep. Usually you are looking for contaminants, deposits, etc. so you look at the sample in the as received condition first. After you have collected what information you can, then you clean the sample to see the fracture surface topography. For metals, it may be acetone or hexanes to remove oil and dirt. There are more aggressive approaches, but the idea is to remove most of the surface contamination without damaging the underlying material. It depends on if the sample was corroded or not. For polymers, the surface may be cleaned with soapy water. Anything harsher can destroy the surface. If the material is not very conductive for SEM, then we gold sputter coat the sample. Usually this is a last resort, because you can't take it back off. You can do a lot with low voltage or in an ESEM.
I can go into more detail if you want. I can post to the server to share with everyone if you could tell me more specifically what you are after. There are numerous books, especially for metals.
Gerald Shulke Materials Engineering Specialist Chrysler LLC
Hi Mark,
TEM: Willilams & Carter, "Transmission Electron Microscopy" New editioin due this June Reimer & Kohl, "Transmission Electron Microscopy: Physics of Image Formation (Springer Series in Optical Sciences)" SEM: Goldstein et al., "Scanning Electron Microscopy and X-Ray Microanalysis" get the latest edition -- 3rd, I think Reimer, "Scanning Electron Microscopy: Physics of Image Formation and Microanalysis (Springer Series in Optical Sciences" 1998, though. General: Sawyer & Grubb, "Polymer Microscopy" 3rd edition For a start. What I have a hard time finding is a reference on specimen preparation for materials science. Be interesting to see if anyone posts such a book.
Phil
} Hello. We have traditionally been a Life Sciences Imaging core facility on } campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding } ourselves leaning more towards the Material Sciences. As such, we would } like to augment our Center's reference library with books relating to the } imaging and analytical side of microscopy } } I was wondering if I could get ideas on basic book requirements that } Material scientists would look at as basic references and might be } considered indispensable in a Materials oriented research center. Thanks for } any help! Mark } } } } Mark Grimson, PhD } Manager, The Imaging Center } c/o The Department of Biological Sciences } Flint and Main } Texas Tech University } Lubbock, TX 79409-3131 } } mark.grimson-at-ttu.edu } 806-742-3722 x235 (Office) } 806-252-3879 (Cell) } 806-742-2963 (FAX) -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OLlgt2000484 for {MicroscopyListserverArchive-at-microscopy.com} ; Fri, 24 Apr 2009 16:47:42 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id n3OLlgOh000482; Fri, 24 Apr 2009 16:47:42 -0500
Randy,
A Sears Craftsman variable speed 3/8th inch drill works just as good, has more low speed range, and takes any size carbon or graphite rod (1/8" to 1/4" or more). I made a sharpened rod one inch long that way with just a Sears drill and a manual sharpener. I also used a fast turning lab scale miniature lathe years ago but the drill works better and is smaller but heavier than a Dremel tool. Kiss sore and black fingers goodbye!
Paul Beauregard
At 11:23 AM 4/24/09 -0500, TindallR-at-missouri.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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From katamail.com-select6-at-myway.com Sat Apr 25 00:30:56 2009 Return-Path: {katamail.com-select6-at-myway.com} Received: from google.com ([84.36.74.94]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3P5UtnI003300 for {microscopylistserverarchive-at-microscopy.com} ; Sat, 25 Apr 2009 00:30:55 -0500 Received: from [150.98.96.94] (HELO google.com) by cozysock.us; Sat, 25 Apr 2009 07:35:25 +0200
These are two good refs for metals:
ASM Metals Handbook Volume 9 Metallography and Microstructures
Metallography Principles and Practice by George Vander Voort
Jeff Stewart Materials Characterization Lab Manager Stern-Leach Company 49 Pearl Street Attleboro, MA 02703 508-222-7400 x1329
On Fri Apr 24 16:21 , oshel1pe-at-cmich.edu sent:
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Do not despair! Fine the organisation local to you that deals with x-ray equipment in industry or hospitals. It is my experience that if you take along the two unique ends of your old high voltage cable they will be able to repair it for you. I did this many times in many different countries and I often found they were so interested to work on a different type of application that this was recognised in the basic fee that they charged!
For me it was always cheaper than obtaining a new cable from Japan, for you it could resurrect your instrument?
Good luck.
Steve
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: RCsencsits-at-lbl.gov [mailto:RCsencsits-at-lbl.gov] Sent: 24 April 2009 21:26 To: protrain-at-emcourses.com
Our high voltage cable went bad on our little old Zeiss EM10, vintage 1976. Would anyone have a spare? Can you recommend a company to make a new one? Thanks,
Roseann Csencsits, PhD Scientist in Charge - Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
==============================Original Headers============================== 2, 23 -- From RCsencsits-at-lbl.gov Fri Apr 24 15:25:11 2009 2, 23 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OKPBAX028666 2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:25:11 -0500 2, 23 -- X-Ironport-SBRS: 2.3 2, 23 -- X-IronPort-Anti-Spam-Filtered: true 2, 23 -- X-IronPort-Anti-Spam-Result: Aj4CAIO88UmAAykYe2dsb2JhbACWTgEBFiIFqUkJj3aCQYEzBQ 2, 23 -- X-IronPort-AV: E=Sophos;i="4.40,243,1239001200"; 2, 23 -- d="scan'208";a="12586616" 2, 23 -- Received: from mta1.lbl.gov ([128.3.41.24]) 2, 23 -- by ironport4.lbl.gov with ESMTP; 24 Apr 2009 13:25:10 -0700 2, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.246]) 2, 23 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id n3OKPADO025737 2, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Apr 2009 13:25:10 -0700 (PDT) 2, 23 -- Message-Id: {EF4BAEF5-F5E7-49C1-B756-C1AB159127BF-at-lbl.gov} 2, 23 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 23 -- Content-Transfer-Encoding: 7bit 2, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 2, 23 -- Subject: HT cable wanted for Zeiss EM10 2, 23 -- Date: Fri, 24 Apr 2009 13:25:10 -0700 2, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 14, 27 -- From protrain-at-emcourses.com Sat Apr 25 14:25:51 2009 14, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3PJPoPd024532 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 14:25:51 -0500 14, 27 -- Received: from 5ad57292.bb.sky.com ([90.213.114.146]:2167 helo=HP6220) 14, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 14, 27 -- (envelope-from {protrain-at-emcourses.com} ) 14, 27 -- id 1LxnVU-000738-2u by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Sat, 25 Apr 2009 21:25:48 +0200 14, 27 -- Reply-To: {protrain-at-emcourses.com} 14, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 14, 27 -- To: {RCsencsits-at-lbl.gov} 14, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 14, 27 -- References: {200904242026.n3OKQEH7031313-at-ns.microscopy.com} 14, 27 -- Subject: RE: [Microscopy] HT cable wanted for Zeiss EM10 14, 27 -- Date: Sat, 25 Apr 2009 20:25:26 +0100 14, 27 -- Organization: Protrain 14, 27 -- Message-ID: {014d01c9c5db$a53a4960$0200a8c0-at-HP6220} 14, 27 -- MIME-Version: 1.0 14, 27 -- Content-Type: text/plain; 14, 27 -- charset="us-ascii" 14, 27 -- Content-Transfer-Encoding: 7bit 14, 27 -- X-Mailer: Microsoft Office Outlook 11 14, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 14, 27 -- Thread-Index: AcnFGvb3MVBJ58D9To2D8eXyfobIVgAv4Tog 14, 27 -- In-Reply-To: {200904242026.n3OKQEH7031313-at-ns.microscopy.com} 14, 27 -- X-Scan-Signature: d8053df69de9c664bd038f8acb29a810{81}} 14, 27 -- X-Trace: smtp01.dial-up.net 1LxnVU-000738-2u dde184db835de61af203cf8137bad510 ==============================End of - Headers==============================
I have been following this tread and I wanted to reach out regarding the subject of materials.
I am an Graduate Artist at New York University doing research on art/science collaborations. Using the artistic medium to stimulate and educate the viewer about scientific fields that affect their everyday lives. Clearly material science is one such field. I am interested in large SEM fracture topology images of Aluminum alloy. I have been told that AL has a very interesting crystalline structure plus the use of AL in aircraft is a direct (and visceral) connection to the general public. The end use of this image data would be constructing a large scale sculpture of the fracture topology. Vector (z-depth) data would be most helpful for construction, since I could port it into a 3-d software package (has anyone done this?), but I was informed that STM microscopy is not often used for fracture analysis.
I am sure all of you have seen amazing things on the micro-scale, Ideas and comments would be appreciated. I am looking for collaboration.
Cheers,
Brian Jones ITP 721 Broadway, 4th Floor New York, NY 10003 http://itp.nyu.edu
----- Original Message ----- X-from: jeff-at-metallography.com
Brian,
It looks like you are interested in materials and there is a lot of fascinating topology. But have you considered microelectronics? I have seen some amazing SEM images of VLSI MOSFET technology and we use this technology in our everyday lives.
Richard
-- ........................................................................ Richard E. Stallcup II, PhD Applications Manager, NanoWorks® Tools; Senior Scientist
The information contained in this transmission is privileged and confidential and is intended only for the use of the addressee(s).
This e-mail is sent for business reasons only and should be considered confidential. ........................................................................
==============================Original Headers============================== 10, 24 -- From rstallcup-at-zyvex.com Sat Apr 25 22:13:23 2009 10, 24 -- Received: from thebe.zyvex.com (gw.zyvex.com [24.173.8.226]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3Q3DNLj009114 10, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 22:13:23 -0500 10, 24 -- Received: from [192.168.1.100] (67-198-24-182.dyn.grandenetworks.net [67.198.24.182]) 10, 24 -- (authenticated bits=0) 10, 24 -- by thebe.zyvex.com (8.13.1/8.13.1) with ESMTP id n3Q3DImf006520 10, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Apr 2009 22:13:19 -0500 10, 24 -- Message-ID: {49F3D14E.6040601-at-zyvex.com} 10, 24 -- Date: Sat, 25 Apr 2009 22:13:18 -0500 10, 24 -- From: Richard Stallcup {rstallcup-at-zyvex.com} 10, 24 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 10, 24 -- MIME-Version: 1.0 10, 24 -- To: Microscopy-at-microscopy.com 10, 24 -- Subject: [Microscopy] Basic Materials TEM/SEM/LM book ideas 10, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-Spam-Status: No, score=0.1 required=5.0 tests=RDNS_DYNAMIC 10, 24 -- autolearn=disabled version=3.2.5 10, 24 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on thebe.zyvex.com 10, 24 -- X-Virus-Scanned: ClamAV 0.93.3/9288/Fri Apr 24 21:22:05 2009 on thebe.zyvex.com 10, 24 -- X-Virus-Status: Clean 10, 24 -- Received-SPF: pass (thebe.zyvex.com: 67.198.24.182 is authenticated by a trusted mechanism) ==============================End of - Headers==============================
Hi listers, I noticed that after the advent of thin bar grids, sections didn't stick as well, probably because there was less surface area for sections to adhere to. Like Pat Common, I just put them in a 50 degree oven and never lose sections. 30 minutes works but we've left them in there inadvertently for several days, and they are fine. No pre-cleaning is necessary. An absolute ethanol dip does seem to minimize the tendency for sections to "run away" and also cuts down on what I call the jello water effect.
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 Ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 FAX (859) 257-1581 University of KY Lexington, KY 40536
==============================Original Headers============================== 3, 24 -- From mgengle-at-email.uky.edu Mon Apr 27 08:17:42 2009 3, 24 -- Received: from ironporta.uky.edu (ironporta.uky.edu [128.163.184.75]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RDHd52029806 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 08:17:41 -0500 3, 24 -- Received: from ex7hb04.ad.uky.edu ([128.163.187.54]) 3, 24 -- by ironporta.uky.edu with ESMTP; 27 Apr 2009 09:17:35 -0400 3, 24 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB04.ad.uky.edu 3, 24 -- ([128.163.187.54]) with mapi; Mon, 27 Apr 2009 09:17:36 -0400 3, 24 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 3, 24 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 3, 24 -- Date: Mon, 27 Apr 2009 09:17:34 -0400 3, 24 -- Subject: sections and grids 3, 24 -- Thread-Topic: sections and grids 3, 24 -- Thread-Index: AcnHOofDxkPdDT1mQVOpDKiqQQVpLw== 3, 24 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A857095BBC1AA5-at-EX7FM03.ad.uky.edu} 3, 24 -- Accept-Language: en-US 3, 24 -- Content-Language: en-US 3, 24 -- X-MS-Has-Attach: 3, 24 -- X-MS-TNEF-Correlator: 3, 24 -- acceptlanguage: en-US 3, 24 -- Content-Type: text/plain; charset="us-ascii" 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Transfer-Encoding: 8bit 3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3RDHd52029806 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Interdisciplinary Symposium on 3D microscopy 2009
Question: Dear Colleagues,
The "Interdisciplinary Symposium on 3D microscopy 2009" will take place in Interlaken, Switzerland, between 12th and 16th of July.
This meeting will be an exiting forum to meet with scientists working in all fields of microscopy using fascinating techniques to study 3 dimensional objects. We would like to encourage you to participate to this meeting and contribute to its success by bringing new ideas and innovations that you are using or plan to use in this field.
World renown speakers and experts will talk about the state of the art in all different disciplines.
Suggested Topics: High resolution TEM and AFM 3D CLSM and Light Microscopy Stereology 3D TEM Tomography and Serial Sectioning 3D X-ray Microscopy and Tomography 3D FIB/SEM or Serial Sectioning 3D Image Analysis and simulation Scanning Probe Microscopy
Plenary Lectures: Free electron laser (XFEL); Travelling-wave MRI and Scanning Force Microscopy on Mars
Further interesting topics such as atom probe will also be treated.
Organizing committee and chairpersons are: M. Cantoni, M. D¸rrenberger, C. Genoud, L. Holzer, M. Ochs, M. Stampanoni, U. Staufer, R. Wepf and S. Abolhassani
I would like to invite you to visit the following link to know more about this conference and welcome you to participate to this event.
http://www.ssom.ch/3D/index.html
On behalf of the organizing committee,
Dr. Sousan Abolhassani Paul Scherrer Institut 5232 Villigen-PSI Switzerland
==============================Original Headers============================== 18, 13 -- From zaluzec-at-microscopy.com Mon Apr 27 08:21:47 2009 18, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 18, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RDLkx7030954 18, 13 -- for {microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 08:21:47 -0500 18, 13 -- Mime-Version: 1.0 18, 13 -- Message-Id: {p06240801c61b61d4b6ef-at-[206.69.208.22]} 18, 13 -- Date: Mon, 27 Apr 2009 08:21:46 -0500 18, 13 -- To: microscopy-at-microscopy.com 18, 13 -- From: sousan.abolhassani-at-psi.ch (by way of MicroscopyListserver) 18, 13 -- Subject: viaWWW: Interdisciplinary Symposium on 3D microscopy 2009 18, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 18, 13 -- Content-Transfer-Encoding: 8bit 18, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3RDLkx7030954 ==============================End of - Headers==============================
From tuckertwct-at-aol.com Mon Apr 27 09:59:16 2009 Return-Path: {tuckertwct-at-aol.com} Received: from google.com (221-172.skknet.net [85.11.172.221] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RExDeS029669 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 27 Apr 2009 09:59:15 -0500 Received: from [141.81.20.83] (HELO google.com) by thinyclammed.de; Mon, 27 Apr 2009 17:59:15 +0300 Message-ID: {000000031EFF2D9431498818} Reply-To: Phemie Dull {meade.tarring15632-at-gmail.com}
AFM in Biology Class Asylum Research, Santa Barbara, CA June 3-5
This comprehensive class is open to all AFM scientists that wish to expand their AFM knowledge as it pertains to life science applications. The class includes both lecture and extensive hands-on experiments with topics on:
• Basic AFM operation (as demonstrated on the MFP-3D AFM) • Biological sample preparation and interpretation of AFM data • Choice of cantilevers • Imaging samples in air and fluids: from molecules to cells • Force measurements: intra molecular forces and hardness measurements • Simultaneous AFM and optical microscopy techniques including fluorescence and phase contrast • Recognizing artifacts
Additional information can be found at www.asylumresearch.com.
Terry Mehr Asylum Research
==============================Original Headers============================== 6, 13 -- From terry-at-AsylumResearch.com Mon Apr 27 12:18:34 2009 6, 13 -- Received: from exchange.AsylumResearch.com (exchange.asylumresearch.com [207.154.79.129]) 6, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RHIXj5004087 6, 13 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Apr 2009 12:18:33 -0500 6, 13 -- Date: Mon, 27 Apr 2009 10:18:30 -0700 6, 13 -- From: Terry Mehr {terry-at-AsylumResearch.com} 6, 13 -- User-Agent: Thunderbird 2.0.0.6 (Macintosh/20070728) 6, 13 -- MIME-Version: 1.0 6, 13 -- To: Microscopy-at-microscopy.com 6, 13 -- Subject: AFM in Biology Class June 3-5, 2009 6, 13 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 13 -- Content-Transfer-Encoding: 8bit 6, 13 -- Message-ID: {1LyUTP-0000Ik-7Z-at-exchange.AsylumResearch.com} ==============================End of - Headers==============================
From whdvch-at-hotmail.com Mon Apr 27 14:30:04 2009 Return-Path: {whdvch-at-hotmail.com} Received: from google.com (88-109-253-202.dynamic.dsl.as9105.com [88.109.253.202]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3RJU2pi025127 for {microscopylistserverarchive-at-microscopy.com} ; Mon, 27 Apr 2009 14:30:03 -0500 Received: from [187.114.40.181] (HELO google.com) by spottedmill.net; Mon, 27 Apr 2009 20:30:04 +0100 Message-ID: {00000002ED826B3869110148} Reply-To: Mordecai Wiggins {18553weston.hillerud-at-gmail.com}
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Email: erin-at-aaisolutions.com Name: Erin Curry
Organization: AAI
Title-Subject: [Filtered] SmartScope Flare 200 needs new home
Question: Hello, We have an Optical Gaging Smartscope Flare 200 video metrology system available, complete with PC, software, and antivibration platform. Please contact erin-at-aaisolutions.com for photos and additional details. Thanks, Erin
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Email: rnichols-at-bcm.edu Name: ralph nichols
Organization: Baylor College of Medicine
Title-Subject: [Filtered] GMA
Question: Hello Listers
I have project that involve GMA (glycomethacrylate) blocks. The investigator wants to do TEM on blocks after light microscopy has been done. My question is there any procedures to remove GMA from tissue to do TEM.
Thanks
Ralph Nichols Baylor College of Medicine Houston TX
It was my understanding of using / having once or twice used GMA (glycol-methacrylate resin i.e. as Technovit 7100) a long time ago that either LM as well as EM can/could be done on the same block without attempting or necessity/possibility to remove the resin first for ultrathin sectioning. Such a task implies to use another formulation ("hard")from the initial stage rather than a resin mixing formula used only for LM-application(s). Removal of resin seems to be / is impossible ( cf. e.g. Histonet-communication and other sources on that subject http://www.histosearch.com/histonet/Feb06/RE.HistonetTechnovit7100rB.html )
GMA in some way behaves differently from Epoxy-type resins (in terms of hardness/brittleness, section properties etc., etc.) but as far as to my knowledge it would be possible to apply directly "histological-histochemical stainings" as well as EM-heavy metal contrast /staining solutions on to ultrathin sections.
Perhaps it will be of help to visit a website at SPI which seems to contain more specified if not comprehensive information about the stuff you should work with (if you do not have access to the original "old" literature/papers):
Also, you could search in the URLs-Index of http://www.ebsciences.com/papers/index.html :
Initial statement there: NOTE: GMA resin-based products (Technovit® 7100, 8100) should not be used when epoxy removal from specimens is required. There is no known method of GMA epoxy removal (including methoxide) that does not effectively render specimens unusable. Instead, MMA resin-based products such as Technovit® 9100 should be selected
cf. also Section: Glycol Methacrylate: Embedding and Staining Technovit® 7100 and 8100 Application Information from Heraeus Kulzer Embedding Protocols and Methyl Methacrylate: Embedding and Staining Technovit® 9100 Application Information from Heraeus Kulzer and microtomy -at- http://www.ebsciences.com/histology/methacrylate.htm#5
Usual Disclaimer applies for companys mentioned in URL's....no personal affiliation, no interest...
The real question for me is left unanswered: why it is necessary for you/your client to remove GMA resin for TEM sectioning/staining at all ?
Best wishes and good luck,
Wolfgang Muß Salzburg-Austria
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal
=========================================== OR Dr. phil. Wolfgang Muss Head of EM-Lab Institute of Pathology, SALK-LKH (Salzburger Landeskliniken gemeinnuetzige GesmbH, Landeskrankenhaus = Fed. State Gen. Hosp.) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively (same Lab, same address)
Paracelsus Medical Private University (PMU) Univ.-Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work: +43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.Muss-at-aon.at
Ankuendigung namens der (Information on behalf of) Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org {
------------------------------------------------------------------------- Forthcoming in 2009: +++2009, June 11th - June 13th: 36th Ann. SCUR Meeting (SCUR meets Florence), Host: Francesca Prignano and her team+++ We cordially invite you to participate actively in the meeting. Visit: Next Meetings at: http://orgs.dermis.net/content/e04scur/e03meetings/e770/e771/index_ger.html
} -----Ursprüngliche Nachricht----- } Von: rnichols-at-bcm.edu [mailto:rnichols-at-bcm.edu] } Gesendet: Dienstag, 28. April 2009 00:23 } An: Muß Wolfgang } Betreff: [Microscopy] GMA (glycolmethacrylate) blocks for LM-followed by TEM? (removal of GMA?)
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==============================Original Headers============================== 33, 36 -- From W.Muss-at-salk.at Tue Apr 28 03:00:06 2009 33, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 33, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3S804Ss015022 33, 36 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 03:00:05 -0500 33, 36 -- Received: from localhost (localhost [127.0.0.1]) 33, 36 -- by hermes.salk.at (Postfix) with ESMTP id 3CB8CC385F; 33, 36 -- Tue, 28 Apr 2009 10:00:02 +0200 (CEST) 33, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 33, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 33, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 33, 36 -- with ESMTP id jzcXWAeQ+x35; Tue, 28 Apr 2009 10:00:01 +0200 (CEST) 33, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 33, 36 -- by hermes.salk.at (Postfix) with ESMTP id C36EDC3850; 33, 36 -- Tue, 28 Apr 2009 10:00:01 +0200 (CEST) 33, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 33, 36 -- Tue, 28 Apr 2009 10:00:01 +0200 33, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 33, 36 -- Content-class: urn:content-classes:message 33, 36 -- MIME-Version: 1.0 33, 36 -- Content-Type: text/plain; 33, 36 -- charset="iso-8859-1" 33, 36 -- Subject: [Microscopy] Re: GMA 33, 36 -- Date: Tue, 28 Apr 2009 10:00:01 +0200 33, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D09D9-at-N1RZ116.lksdom21.lks.local} 33, 36 -- In-Reply-To: {200904272223.n3RMNT1U011115-at-ns.microscopy.com} 33, 36 -- X-MS-Has-Attach: 33, 36 -- X-MS-TNEF-Correlator: 33, 36 -- Thread-Topic: [Microscopy] Re: GMA 33, 36 -- Thread-Index: AcnHhs8iJZp1wjPrRXS6LS98icg1uAAR3vxw 33, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 33, 36 -- To: {microscopy-at-microscopy.com} 33, 36 -- Cc: {rnichols-at-bcm.edu} 33, 36 -- X-OriginalArrivalTime: 28 Apr 2009 08:00:01.0822 (UTC) FILETIME=[553543E0:01C9C7D7] 33, 36 -- X-Scanned-By: SALK-Content-Filter 33, 36 -- Content-Transfer-Encoding: 8bit 33, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3S804Ss015022 ==============================End of - Headers==============================
Here is the May 2009 Microscopy Today table of contents. We will close the subscription list for this issue on Wednesday, April 1, 2009. Sorry for the short time interval as we are trying to beat the May 11th postal rate increase. Microscopists in North America and MSA members anywhere qualify for free subscriptions. All subscriptions at http://www.microscopy-today.com .
The July issue of MT will be totally reformatted with a new look and new features under Charles Lyman, the new editor. Not least of which will be an exact duplicate digital edition available nearly simultaneously. All URLs and emails in the digital edition will be live so that you may go to the desired link from within the digital edition.
Thank you, Ron Anderson, Managing Editor
========================
Developmental Dynamics in Real Time Stephen W. Carmichael, Mayo Clinic
Complexions: A Revolutionary Taxonomy for Grain Boundaries Alwyn Eades, Lehigh University, Bethlehem, PA
Development of a 200kV Atomic Resolution Analytical Electron Microscope T. Isabell*, et al., and I. Ishikawa**, et al. *JEOL USA, Inc., Peabody, MA **JEOL Ltd., Akishima, Japan
Vibration Isolation Critical to Measuring Neuronal Patterns in the Brain David L. Platus, Minus K Technology, Inc., Inglewood, CA
Phase Identification and Mapping Based on Valence Loss EELS and ELNES R.D. Twesten, Gatan, Inc., Pleasanton, CA
Improved Techniques For Imaging Of Three-Dimensional Transparent Specimens In Advanced Darkfield And Interference Contrast Modes Jörg Piper, Clinic Meduna, Bad Bertrich, Germany
Low Energy, Low Angle, Large Area Ion Polishing for Improved EBSD Indexing S.D. Walck*, J.R. Porter**, H-W. Yang**, S.S. Dheda**, *South Bay Technology, Inc., San Clemente, CA, **UC Irvine, CA
Toward Robust High Resolution Chemical Imaging C. A. Barrios, A. V. Malkovskiy, A. Kisliuk, A. P. Sokolov, M. D. Foster, Dept. of Polymer Science, The U. of Akron, Akron, OH
Distinguishing the Data from the Dark: Single Source Software or Microscopy Mix and Match? Tim Oliver, Duke University Medical Center, Durham NC
Pioneers in Optics: Alhazen and Roger Bacon Michael W. Davidson, National High Magnetic Field Laboratory, The Florida State University, Tallahassee, FL
The Electron Gun its Saturation and Alignment—An Old Man’s Saga Steve Chapman, Protrain, Buckingham, England
Determining the Micron Marker Distance or Magnification of a Microscopic Image Paul Beauregard, Chemist and Electron Microscopist, Greensburg, PA
A Very Simple Method for Quickly Making Large Numbers of Measurements on Micrographs Ron Anderson, Microscopy Today, Largo, FL
Dear Abbe
Industry News
NetNotes SPECIMEN PREPARATION – waxy cuticles SPECIMEN PREPARATION – TEM of fossil tooth SPECIMEN PREPARATION – retinas SPECIMEN PREPARATION - SEM of fibrin clots SPECIMEN PREPARATION - liposomes TEM - Oval beam SEM – oil shale samples SEM- magnetic materials SEM – catholuminescence EDX – broken detector window EDX- mothball liquid nitrogen chilled detector Convergent-beam electron diffraction – thickness measurement Advertiser's Index
==============================Original Headers============================== 23, 17 -- From randerson20-at-tampabay.rr.com Tue Apr 28 09:17:40 2009 23, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 23, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3SEHdnS028510 23, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Apr 2009 09:17:40 -0500 23, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 23, 17 -- by hrndva-omta01.mail.rr.com with ESMTP 23, 17 -- id {20090428141739525.OTXR11305-at-hrndva-omta01.mail.rr.com} 23, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Apr 2009 14:17:39 +0000 23, 17 -- Message-ID: {49F70FFB.3030407-at-tampabay.rr.com} 23, 17 -- Date: Tue, 28 Apr 2009 10:17:31 -0400 23, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 23, 17 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 23, 17 -- MIME-Version: 1.0 23, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 23, 17 -- Subject: Microscopy Today May 2009 Table of Contents 23, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 23, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] Scanning electron microscope
Question: Eastern Virginia Medical School owns a Phillips SEM515 scanning electron microscope, with Polaroid model 545 camera and EDAC X-ray dispersive analysis unit attached. It is in the core facility that I direct. It is not currently functioning, but needs about $5000 in repairs. The school would like to give it away, preferably to a non-profit organization.
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Email: vwporsche-at-verizon.net Name: Rod Rowland
Organization: MATSYS
Title-Subject: [Filtered] Looking for Literature JEOL SEM 6100
Question: I just acquired a used JEOL SEM model 6100. I did not receive any literature with this SEM and would like to know if anyone can help me find some basic user / setup info such as; operator manual, schematics, etc..?
Well, now you know the principle of through fact finding before purchase or transfer of a SEM. A "good deal" is not necessarily so.
Just advise to others--check the details. If you do not know the details, do your homework. An initial "good deal" could easily become a nightmare afterwards. Ask the list. There is much history out here. It is a rich resource. Personal stuff can be easily off-line.
gary g.
At 07:54 PM 4/28/2009, you wrote:
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==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Tue Apr 28 22:05:18 2009 9, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3T35ITv018776 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 22:05:18 -0500 9, 20 -- Message-Id: {200904290305.n3T35ITv018776-at-ns.microscopy.com} 9, 20 -- Received: (qmail 9631 invoked from network); 28 Apr 2009 20:17:13 -0700 9, 20 -- Received: by simscan 1.1.0 ppid: 9628, pid: 9629, t: 0.1062s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by smtp1 with SMTP; 28 Apr 2009 20:17:13 -0700 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Tue, 28 Apr 2009 20:05:16 -0700 9, 20 -- To: vwporsche-at-verizon.net 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: Documentation on JEOL SEM 6100 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200904290254.n3T2sFNF027867-at-ns.microscopy.com} 9, 20 -- References: {200904290254.n3T2sFNF027867-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Gary, After first doing a more THOROUGH job spell-checking, the hassle (cost) of getting replacement documentation might not be too great compared to a good price on a used SEM. I suspect that Rod won't have too much trouble getting documentation (although I can't help him out with a 6100) and he didn't state what his costs were.
Just my $.02
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Tuesday, April 28, 2009 11:07 PM To: kenconverse-at-qualityimages.biz
Well, now you know the principle of through fact finding before purchase or transfer of a SEM. A "good deal" is not necessarily so.
Just advise to others--check the details. If you do not know the details, do your homework. An initial "good deal" could easily become a nightmare afterwards. Ask the list. There is much history out here. It is a rich resource. Personal stuff can be easily off-line.
gary g.
At 07:54 PM 4/28/2009, you wrote:
} --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Tue Apr 28 22:05:18 2009 9, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3T35ITv018776 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Apr 2009 22:05:18 -0500 9, 20 -- Message-Id: {200904290305.n3T35ITv018776-at-ns.microscopy.com} 9, 20 -- Received: (qmail 9631 invoked from network); 28 Apr 2009 20:17:13 -0700 9, 20 -- Received: by simscan 1.1.0 ppid: 9628, pid: 9629, t: 0.1062s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by smtp1 with SMTP; 28 Apr 2009 20:17:13 -0700 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Tue, 28 Apr 2009 20:05:16 -0700 9, 20 -- To: vwporsche-at-verizon.net 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: Documentation on JEOL SEM 6100 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200904290254.n3T2sFNF027867-at-ns.microscopy.com} 9, 20 -- References: {200904290254.n3T2sFNF027867-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 22, 25 -- From kenconverse-at-qualityimages.biz Wed Apr 29 08:43:59 2009 22, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 22, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3TDhvdL028810 22, 25 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 08:43:58 -0500 22, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com 22, 25 -- with ESMTP 22, 25 -- id {20090429134355144.VDZN22077-at-cdptpa-omta02.mail.rr.com} ; 22, 25 -- Wed, 29 Apr 2009 13:43:55 +0000 22, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 22, 25 -- To: {gary-at-gaugler.com} , "MSA Listserver" {microscopy-at-microscopy.com} 22, 25 -- Subject: RE: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100 22, 25 -- Date: Wed, 29 Apr 2009 09:43:45 -0400 22, 25 -- Message-ID: {238FFDA25F4443849159E381B2CEEDD2-at-Ken} 22, 25 -- MIME-Version: 1.0 22, 25 -- Content-Type: text/plain; 22, 25 -- charset="us-ascii" 22, 25 -- X-Priority: 3 (Normal) 22, 25 -- X-MSMail-Priority: Normal 22, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 22, 25 -- Importance: Normal 22, 25 -- Thread-Index: AcnId6O1n1EXRGQbSV+rflmxFdLLAwAV/Aog 22, 25 -- In-Reply-To: {200904290307.n3T37Lef022759-at-ns.microscopy.com} 22, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 22, 25 -- Content-Transfer-Encoding: 8bit 22, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3TDhvdL028810 ==============================End of - Headers==============================
Hello, I am interested in taking photographs of fish scales and otoliths for age and growth analyses at anywhere between 12.5X to 100X with a Leica DM2000 transmitted light compound microscope. I was hoping to receive advice about the best digital camera and coupler (preferably for $500 or less with an emphasis on the 'less') to purchase for this purpose. I intend to use Image Tool software for the analyses. Any advice concerning use of this program, especially as it pertains to scale & otolith analyses, would also be welcomed.
Thank you very much, Eric
-- Eric R. Huber Carlson Laboratory Manager University of California, Berkeley Dept. of Environmental Science, Policy & Management 140 Mulford Hall #3114 Berkeley, CA 94720 510-643-9688 (office) 508-446-5433 (cell) 510-643-5438 (fax) Office: 304 Mulford Hall http://nature.berkeley.edu/carlsonlab/ http://nature.berkeley.edu/~ehuber/
==============================Original Headers============================== 4, 27 -- From ehuber-at-nature.berkeley.edu Wed Apr 29 16:36:29 2009 4, 27 -- Received: from nature.Berkeley.EDU (nature.Berkeley.EDU [169.229.201.201]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3TLaSha003453 4, 27 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 16:36:28 -0500 4, 27 -- Received: from localhost (localhost [127.0.0.1]) 4, 27 -- by nature.Berkeley.EDU (Postfix) with ESMTP id 99C9A364A7 4, 27 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2009 14:36:28 -0700 (PDT) 4, 27 -- Received: from nature.Berkeley.EDU ([127.0.0.1]) 4, 27 -- by localhost (nature.berkeley.edu [127.0.0.1]) (amavisd-maia, port 10024) 4, 27 -- with ESMTP id 00152-04-3 for {microscopy-at-microscopy.com} ; 4, 27 -- Wed, 29 Apr 2009 14:36:26 -0700 (PDT) 4, 27 -- Received: from [192.168.0.198] (mul-334a-304-007-d.ESPM.Berkeley.EDU [128.32.224.96]) 4, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 27 -- (No client certificate requested) 4, 27 -- (Authenticated sender: ehuber-at-nature) 4, 27 -- by nature.Berkeley.EDU (Postfix) with ESMTPSA id A469F36466 4, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 29 Apr 2009 14:36:26 -0700 (PDT) 4, 27 -- Message-ID: {49F8C85C.5060008-at-nature.berkeley.edu} 4, 27 -- Date: Wed, 29 Apr 2009 14:36:28 -0700 4, 27 -- From: Eric Huber {ehuber-at-nature.berkeley.edu} 4, 27 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 4, 27 -- MIME-Version: 1.0 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- Subject: Need advice on consumer digital camera & Image Tool 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- X-Virus-Scanned: Maia Mailguard 1.0.2 ==============================End of - Headers==============================
Are you saying that my feedback is faulty because of spelling? Perfeck spelling was not my intent. The issue is getting all the docs with a "new" system.
For example, Zeiss will not provide schematics. They will provide maintenance docs, but these are bound to the requestor...they are not transferable. And they have some limit of useability.
If I were to quibble about all the spelling errors on postings, I would be overloaded. So, what is your point? Bad spelling means bad input? How can this be proven? What is your point?
garrry g.
At 06:43 AM 4/29/2009, you wrote: } Gary, } After first doing a more THOROUGH job spell-checking, the hassle (cost) of } getting replacement documentation might not be too great compared to a good } price on a used SEM. I suspect that Rod won't have too much trouble getting } documentation (although I can't help him out with a 6100) and he didn't } state what his costs were. } } Just my $.02 } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Tuesday, April 28, 2009 11:07 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Re: viaWWW: Documentation on JEOL SEM 6100 } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Can anyone recommend a reliable 3rd party service company for a LEO906E TEM, that services the Dallas/Ft. Worth area.
Thanks, Steve
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==============================Original Headers============================== 4, 28 -- From StevenLe-at-BaylorHealth.edu Thu Apr 30 07:52:58 2009 4, 28 -- Received: from bhdappagnt02.baylorhealth.edu (mailhost1.baylorhealth.edu [65.248.93.160]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3UCqweD006313 4, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 07:52:58 -0500 4, 28 -- Received: from BHDAEXIMS02.bhcs.pvt ([10.100.14.73]) 4, 28 -- by bhdappagnt02.baylorhealth.edu (8.14.1/8.14.1) with ESMTP id n3UCqwXK029668 4, 28 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 07:52:58 -0500 4, 28 -- X-TM-IMSS-Message-ID: {330781c00006db01-at-bhcs.pvt} 4, 28 -- Received: from BHDAEXHT02.bhcs.pvt ([10.5.12.191]) by bhcs.pvt ([10.100.14.73]) with ESMTP (TREND IMSS SMTP Service 7.0; TLS: TLSv1/SSLv3,128bits,RC4-MD5) id 330781c00006db01 ; Thu, 30 Apr 2009 07:52:57 -0600 4, 28 -- Received: from BHDAEXVM32.bhcs.pvt ([10.5.3.122]) by BHDAEXHT02.bhcs.pvt 4, 28 -- ([10.5.12.191]) with mapi; Thu, 30 Apr 2009 07:52:57 -0500 4, 28 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu} 4, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 4, 28 -- Date: Thu, 30 Apr 2009 07:52:56 -0500 4, 28 -- Subject: 3rd Party TEM Service 4, 28 -- Thread-Topic: 3rd Party TEM Service 4, 28 -- Thread-Index: AcnJkpVygTROcHn6RB69vtO4cOuIqA== 4, 28 -- Message-ID: {492DD4D34D5DC5489E59D73B0506681C0CDD80CAAA-at-BHDAEXVM32.bhcs.pvt} 4, 28 -- Accept-Language: en-US 4, 28 -- Content-Language: en-US 4, 28 -- X-MS-Has-Attach: 4, 28 -- X-MS-TNEF-Correlator: 4, 28 -- acceptlanguage: en-US 4, 28 -- Content-Type: text/plain; charset="us-ascii" 4, 28 -- MIME-Version: 1.0 4, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-04-30_07:2009-04-28,2009-04-30,2009-04-29 signatures=0 4, 28 -- Content-Transfer-Encoding: 8bit 4, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n3UCqweD006313 ==============================End of - Headers==============================
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Email: david.mitchell-at-emu.usyd.edu.au Name: David Mitchell
Organization: EM Unit, University of Sydney
Title-Subject: [Filtered] TEM: Magnetic Field Cancellation Systems
Question: Dear All
We are currently commissioning a new 200kV TEM/STEM with an in-column filter. The room only just met environmental specs with respect to magnetic field levels, and the new instrument does meets performance specs in terms of STEM resolution. However, I think it could be better. A fixed probe at 1 million times does show evidence of external magnetic field interference. These is some jitter in the 1-10Hz range of about 1nm amplitude, and there is much lower frequency drift over longer time frames of } 1 minute. We have measured AC fields in various planes, at typically 1mG or less, but up to 2.2mG in some instances. I have used a Lundgren field compensation system in a lab which was way out of spec (15mG), and so realise that they can make a huge difference in those situations. However, does anyone have experience with such systems in relatively low field environments such as I have? Is any potential improvement in STEM performance going to be worth the ca $50k investment?
I have been given human fat samples and need to embed them in paraffin. In the past I've used a VIP processor for this and now I have an Autotechnicon (vintage dual model) with a timing wheel.
I know I need to process these fatties slowly, my question is--can I use 2 hours per step and have it turn out OK? I have a timing wheel punched out for 2 hour steps.
My steps would be alcohols: 70, 80, 95 x 2, 100 x 3, Citrisolv x3, paraffin x2 and another paraffin step under vacuum.
Let me know your wise and experienced opinions or protocols. I don't have anything else to use except the 43 year old Autotechnicon so don't even suggest it. You make me feel bad that I can't get my research foundation to buy me something new or even newer. ;-)
Stewing in somebody else's fat (eew!),
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 12, 26 -- From vapatpxs-at-yahoo.com Thu Apr 30 17:22:13 2009 12, 26 -- Received: from n70.bullet.mail.sp1.yahoo.com (n70.bullet.mail.sp1.yahoo.com [98.136.44.38]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3UMMAK4018582 12, 26 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 17:22:11 -0500 12, 26 -- Received: from [69.147.84.144] by n70.bullet.mail.sp1.yahoo.com with NNFMP; 30 Apr 2009 22:22:08 -0000 12, 26 -- Received: from [69.147.84.114] by t6.bullet.mail.sp1.yahoo.com with NNFMP; 30 Apr 2009 22:22:08 -0000 12, 26 -- Received: from [127.0.0.1] by omp203.mail.sp1.yahoo.com with NNFMP; 30 Apr 2009 22:22:08 -0000 12, 26 -- X-Yahoo-Newman-Property: ymail-3 12, 26 -- X-Yahoo-Newman-Id: 601078.46609.bm-at-omp203.mail.sp1.yahoo.com 12, 26 -- Received: (qmail 64809 invoked by uid 60001); 30 Apr 2009 22:22:08 -0000 12, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1241130128; bh=ohjqwVa2mO8sAN+4pKRYxvmo3Luj25r6Dq2LNtxTXyg=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; b=pBxxnkyEjuT4zjWH9bjjkeHcLjnavNOTOIH7jkK1NrYyVRvOW31rCrF+mCKsB07WBCOq6g0bIHvRyVVka4+QLSsOBaPEgVFZo7lSO+2AV3RhyuxTJ2YLkALJJSkTRACyTMVdQABgkudo5mZyTA+AGRY41bkz60HACZJtkQkTKiQ= 12, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 26 -- s=s1024; d=yahoo.com; 12, 26 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type; 12, 26 -- b=hUH+Hn7j8VVbcVgY87bx9qhOR4YatWOaquwmOv4ZsQySssQ8bKVDu+zySPbeGHHb8teSF1bwUFvED9o8PFEJzBTngkEmrBC3+AVdnZ10YVAL1dBabPPBLFdeZAE0r32XwAqGkRb8WwSmkFbVzzfu/0VHjSQLHI4U0bj2Zfu0DsU=; 12, 26 -- Message-ID: {490013.63581.qm-at-web46103.mail.sp1.yahoo.com} 12, 26 -- X-YMail-OSG: ETKpnmsVM1l7e3kaiji5LPs16AnfoB9Zql9hjT3KX680ZwmIJYW8Ey7rGbPcPlQaqEp5pKnNyI3EjjYeB2dq.wWt1Yqjm8vIGoPyF1ksBDuIPbNf7h3.GNNht398tZ2w63H52s_zaWx54N.hxq69UsJbsoZj4yjXU05RLgXh48iKoNCDQ882WSyv_Z6lYb3OjdXlt2PepB6.BE5Hj99dLxbuwspWKt3zQpBegGSaoSGjciAs4m4E0xNs.ul2Wp5uQiFSmQnnpzp24LERelg- 12, 26 -- Received: from [132.239.85.200] by web46103.mail.sp1.yahoo.com via HTTP; Thu, 30 Apr 2009 15:22:08 PDT 12, 26 -- X-Mailer: YahooMailClassic/5.2.20 YahooMailWebService/0.7.289.1 12, 26 -- Date: Thu, 30 Apr 2009 15:22:08 -0700 (PDT) 12, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 12, 26 -- Subject: Processing Fat for Paraffin 12, 26 -- To: HistoNet {histonet-at-lists.utsouthwestern.edu} , 12, 26 -- MSA BB {Microscopy-at-microscopy.com} 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
For users of the Hitachi S-4700 SEM, I would appreciate feedback about what you think about this system.
} } Does the S-4700 come with an operator's manual, schematics } } and maintenance manual? Does Hitachi or you change } } the tip? What is the cost of the tip and/or the } } maintenance contract? How often do you have to } } flash the tip? Do you have the bakeout materials? Have you used this? } } } } What are the dry pumps for this system if you have them? What } } OS and GUI version and generation are in the } } system? What is the max digital pixel capture values? } } } } Is there a separate user and service login? If so, do } } you have the service login password? What is the maintenance } } history of your tool? I.e., down a lot or up a lot?
Do you have EDS and how satisfied are you with the SEM's ability to do EDS? What is the analytical WD for EDS?
Does the specimen interchange load lock work well? Does it support FEI/LEO/Amray/Zeiss 3.1mm pin stubs without a lot of hassle?
What does a basic maintenance contract cost and what does it cover? If you don't have a contract, how responsive is Hitachi to your specific problems that you cannot fix? What about GUI upgrades? Can you get these without a contract?
Finally, what do you think about the cold FE versus the Schottky FE? Have you had problems with stability for lengthy EDS mapping?
All input appreciated--off-line is probably preferred.
gary g.
==============================Original Headers============================== 11, 17 -- From gary-at-gaugler.com Thu Apr 30 18:52:33 2009 11, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n3UNqU3k003280 11, 17 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 18:52:32 -0500 11, 17 -- Message-Id: {200904302352.n3UNqU3k003280-at-ns.microscopy.com} 11, 17 -- Received: (qmail 3591 invoked from network); 30 Apr 2009 17:04:40 -0700 11, 17 -- Received: by simscan 1.1.0 ppid: 3587, pid: 3588, t: 0.0802s 11, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 17 -- by smtp1 with SMTP; 30 Apr 2009 17:04:40 -0700 11, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 17 -- Date: Thu, 30 Apr 2009 16:52:28 -0700 11, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 11, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 17 -- Subject: Hitachi S-4700 experiences 11, 17 -- Mime-Version: 1.0 11, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
A postdoctoral position and a graduate student position are available in the group of Henning Stahlberg in the laboratory for Cellular Imaging and Nano Analytics at the University Basel in Switzerland. The project involves the 3D structural analysis of several samples from the areas of neurology and bacterial infection biology, using cryo-electron tomography and computer image processing as major methods. The positions are available immediately, long-term funding is secured. Equipment includes a FEI Titan Krios with autoloader, 4K CCD and GIF, as well as several other instruments. Basel is a culturally rich and beautiful city at the border between Switzerland, France and Germany. For further information, please contact Henning Stahlberg (Henning.Stahlberg-at-unibas.ch , +41-61-387 32 62).
Title-Subject: [Filtered] Basic Confocal Workshop -at- University of South Carolina
Question: There are still some slots available in this year's Basic Confocal Workshop hosted by the University of South Carolina. This year's workshop will be from June 15-19, 2009 and will include a series of lectures on the theory and applications of confocal microscopy, specimen preparation, processing confocal images in Photoshop, and 3D reconstructions using AMIRA. Students will be able to process triple labeled samples (cell cultures and sections) on site or bring their own samples to the workshop.
Faculty will include Drs. Jay Jerome (Vanderbilt), Ralph Albrecht (Univ Wisconsin-Madison), John Mackenzie (North Carolina State Univ), Tom TrusK (Medical Univ South Carolina) and myself.
Instruments and applications experts from Leica, Nikon, Olympus, Perkin Elmer, Photometrics, and Zeiss will be available for hands on training and imaging of samples.
For those contemplating instrumentation proposals as part of the stimulus or other funding opportunities this is an excellent opportunity to see several systems side by side and to collect preliminary data on their instrument of choice.
For further information and registration go to: http://dba.med.sc.edu/irf/price/irf/irf.htm or contact Anna McNeal (Anna.McNeal-at-uscmed.sc.edu {mailto:Anna.McNeal-at-uscmed.sc.edu} )
Bob
Bob Price Research Professor Dept Cell Biol and Anat USC School of Medicine 6439 Garner's Ferry Road Columbia, SC 29208 Tel: 803-733-3392 Admin Tel: 803-253-5822 Fax: 803-733-3212
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mcintyre-at-optics.rochester.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mcintyre-at-optics.rochester.edu Name: Brian McIntyre
Organization: Univ of Rochester
Title-Subject: [Filtered] Student Web Page Projects
Question: Hi all-
As usual our Electron Microscopy class projects have been posted on our webserver. If you'd like to take a look go here:
==============================Original Headers============================== 9, 11 -- From zaluzec-at-microscopy.com Sat May 2 08:33:07 2009 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n42DX6YA016246 9, 11 -- for {microscopy-at-microscopy.com} ; Sat, 2 May 2009 08:33:06 -0500 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p06240800c621fbda7864-at-[206.69.208.22]} 9, 11 -- Date: Sat, 2 May 2009 08:33:05 -0500 9, 11 -- To: microscopy-at-microscopy.com 9, 11 -- From: mcintyre-at-optics.rochester.edu (by way of MicroscopyListserver) 9, 11 -- Subject: viaWWW: Student Web Page Projects 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
From li_song33-at-excite.com Sun May 3 04:16:24 2009 Return-Path: {li_song33-at-excite.com} Received: from google.com (fla2404-015.wind.ne.jp [218.223.57.15]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n439GMuv013732 for {microscopylistserverarchive-at-microscopy.com} ; Sun, 3 May 2009 04:16:23 -0500 Received: from [125.92.74.69] (HELO google.com) by hollow-wisehead.us; Sun, 3 May 2009 18:16:21 +0900
I'm shooting electron micrographs of mostly planar structures on an IC which I assume is some kind of RAM. Initially I started looking at microprocessors, and, on-line I had found a number of diagrams that labeled areas of the microprocessor as SRAM, DRAM, ALU, etc. I switched to RAM assuming the same information would be readily available for RAM, i.e. some type of equivalent lay-out of various RAM chips at different magnifications but cannot find anything like the microprocessor diagrams:
Ditto light microscopy along the lines of fly logic, a reverse engineering firm that etches and uncovers, then images the various layers of chips:
http://www.flylogic.net/blog/?p=32
I figured all I have to know is where's the input area/encoding/memory/decoding/output, but I didn't translate this into identifying these areas from circuitry.
The chip has the number 7025 on it, and its metal layers number is 105, and it was probably made in 1994 or 1995. In the SE image number 0002 the upper left, where the pads are, is the middle of the chip, and, the large main memory area of the chip is the dark area on the bottom central third of the micrograph.
Can anyone tell me any information about these micrographs? Or where I can find information about planar RAM circuitry at various magnifications? Or if I'm even asking the right questions? I put some micrographs on the web at:
http://www.djwatt.com/
I did not first adjust to web resolution, so, to look at them as micrographs, it might be better to just copy and paste in a word document as they appear awful large on the screen. They should paste as nice 4" high micrographs into some type of document.
Probably off-line responses are better.
Thanks, Kleo
KLeoPullin-at-pacbell.net
==============================Original Headers============================== 14, 21 -- From kleopullin-at-pacbell.net Sun May 3 19:44:59 2009 14, 21 -- Received: from web83408.mail.sp1.yahoo.com (web83408.mail.sp1.yahoo.com [69.147.64.56]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n440iweU009479 14, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 3 May 2009 19:44:59 -0500 14, 21 -- Received: (qmail 24041 invoked by uid 60001); 4 May 2009 00:44:58 -0000 14, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=pacbell.net; s=s1024; t=1241397898; bh=VK0hFu/6ztRFxM+U/877rwx/viqiImV435xv9gHsQx4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; b=VnOz6r0lGsz3UMupMhceznL+Y0XtzdP3LNFWdXVnLWt5jCYpjS8zIy/AyN7BaQg+KXOFvtT2q0hXe8neOSCm45wgRwQynvFxKmsL9BIOrxa6O2zM5FKCELLLzqcS21E+53+2pA0TILkWDDidW3TIXbBIEObPq48Tgr+VrCkx5RM= 14, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 21 -- s=s1024; d=pacbell.net; 14, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type; 14, 21 -- b=xmV3ZapcGz43FoAJNS2lg/yGfEUbp1QmnBuz8qJpEWVdNrzgU0f+5KknWhTI0oHSy90zGoLfE6x804Blk93F8070gdPI+jh4pTGiAsk93Mv8GmLiYBYEm5YMzalZJo61U8k5S5JdfIyM/nXZ8klmCLxYgL/dM72a1atKfvQHtzw=; 14, 21 -- Message-ID: {492913.22296.qm-at-web83408.mail.sp1.yahoo.com} 14, 21 -- X-YMail-OSG: YBAwSoYVM1mOO_TCUkhz_xu83MqTadf0Rno0.DjkhVbE3zcTR98XfvHMj0N_grLTTuDSg5653ELpXAego5g1m3YzUsA2WQ_.6.9M6rpBxtqULeFwAf965MoWAbgJ5IUcmSn6czfzVm7Vul3GtCMw_f9u10w1RHOe2Wpk21VdM_7yKvz.N0Lof.yGunGac8_te9ddjIBnNBZqMyTr2GQcz7CYL4YEEjcrvbi7dLi9y9Bzwbzwbt0lhon_ckX9s1jRG._suegreka48BUxn5ynrYI8I0xthQA24avylLl7K7wu2NEAlbuidnhMJS0H.Eugbe.G.eVtnQrnmwK5SrXZRfU- 14, 21 -- Received: from [69.226.103.38] by web83408.mail.sp1.yahoo.com via HTTP; Sun, 03 May 2009 17:44:58 PDT 14, 21 -- X-Mailer: YahooMailWebService/0.7.289.1 14, 21 -- Date: Sun, 3 May 2009 17:44:58 -0700 (PDT) 14, 21 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 14, 21 -- Reply-To: kleopullin-at-pacbell.net 14, 21 -- Subject: Planar structures on IC - RAM 14, 21 -- To: Microscopy-at-microscopy.com 14, 21 -- MIME-Version: 1.0 14, 21 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
Not a book (the book is in french to my knowledge but the english version is expected in august 2009) but a good web site for the preparation of TEM samples: http://temsamprep.in2p3.fr/techniques.php?lang=eng
Patrick
} What I have a hard time finding is a reference on specimen } preparation for materials science. Be interesting to see if anyone } posts such a book.
Phil
} Hello. We have traditionally been a Life Sciences Imaging core facility on } campus (SEM, TEM, STEM, confocal, fluorescence, etc) but are finding } ourselves leaning more towards the Material Sciences. As such, we would } like to augment our Center's reference library with books relating to the } imaging and analytical side of microscopy } } I was wondering if I could get ideas on basic book requirements that } Material scientists would look at as basic references and might be } considered indispensable in a Materials oriented research center. Thanks for } any help! Mark } } } } Mark Grimson, PhD } Manager, The Imaging Center } c/o The Department of Biological Sciences } Flint and Main } Texas Tech University } Lubbock, TX 79409-3131 } } mark.grimson-at-ttu.edu } 806-742-3722 x235 (Office) } 806-252-3879 (Cell) } 806-742-2963 (FAX) -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 23 -- From oshel1pe-at-cmich.edu Fri Apr 24 14:07:18 2009 4, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n3OJ7IKc022534 4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 14:07:18 -0500 4, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 23 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n3OJ7Ivo011624 4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Apr 2009 15:07:18 -0400 4, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 23 -- Fri, 24 Apr 2009 15:07:17 -0400 4, 23 -- Mime-Version: 1.0 4, 23 -- Message-Id: {f06240816c617be48e46a-at-[141.209.160.249]} 4, 23 -- Date: Fri, 24 Apr 2009 15:07:11 -0400 4, 23 -- To: Microscopy-at-microscopy.com 4, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 23 -- Subject: Re: [Microscopy] Basic Materials TEM/SEM/ LM book ideas 4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 23 -- X-OriginalArrivalTime: 24 Apr 2009 19:07:18.0060 (UTC) FILETIME=[E3035EC0:01C9C50F] 4, 23 -- X-Canit-CHI2: 0.00 4, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 23 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 23 -- X-CanItPRO-Stream: default 4, 23 -- X-Canit-Stats-ID: 12386593 - 56aaaf500e5c 4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 24 -- From weis183-at-yahoo.fr Mon May 4 04:17:13 2009 11, 24 -- Received: from web24615.mail.ird.yahoo.com (web24615.mail.ird.yahoo.com [212.82.104.172]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n449HCDW010816 11, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 4 May 2009 04:17:13 -0500 11, 24 -- Received: (qmail 81041 invoked by uid 60001); 4 May 2009 09:17:12 -0000 11, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.fr; s=s1024; t=1241428632; bh=a14g+EjRjLXZkVC7S23toqjM/0hZXPyS6Zq25ONqE0E=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=DKUrT1XcbQ10+OqUZRjFi3h88Sc1M4W+bjCsHzyuwK9+gtMPvcI2KqwIBhdy8QCw2r3yIev/DWbDhSmtWS7DNafsQW7B04VU0Q186V+d/Od9XP/3H+PI/s5LwVITCBdwYvdzWdUArSnGGU6CvuadczJqNPpiLDGns3hPC/e/GIY= 11, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 24 -- s=s1024; d=yahoo.fr; 11, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 11, 24 -- b=F+o7WPmcQ5PbsU0Y77kUEGhFn0+0kQAvdVgEmGmasqG++P8IzGn2FRdAC85VzpwxH1DvQP4dWDxswX9AxDDgN5UOb/B8ABg7sIilGjjZ+M+Fzvs8v1W64G0PjbOWnbKEiQ8wZjw2cxuHIz64J0WCZEBMXIodLOKFciD1O0pQvYg=; 11, 24 -- Message-ID: {252345.80778.qm-at-web24615.mail.ird.yahoo.com} 11, 24 -- X-YMail-OSG: YWISGaUVM1nIJJF5mrbjJqIK96AV5rqxv_D0coIy89uJjmhcbagHE0Cs 11, 24 -- Received: from [147.210.80.23] by web24615.mail.ird.yahoo.com via HTTP; Mon, 04 May 2009 02:17:11 PDT 11, 24 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1 11, 24 -- References: {200904241910.n3OJAOB4028375-at-ns.microscopy.com} 11, 24 -- Date: Mon, 4 May 2009 02:17:11 -0700 (PDT) 11, 24 -- From: Patrick Weisbecker {weis183-at-yahoo.fr} 11, 24 -- Subject: Re : [Microscopy] Re: Basic Materials TEM/SEM/ LM book ideas 11, 24 -- To: Microscopy-at-microscopy.com 11, 24 -- In-Reply-To: {200904241910.n3OJAOB4028375-at-ns.microscopy.com} 11, 24 -- MIME-Version: 1.0 11, 24 -- Content-Type: text/plain; charset=iso-8859-1 11, 24 -- Content-Transfer-Encoding: 8bit 11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n449HCDW010816 ==============================End of - Headers==============================
Kleo, The description you provided for main portions of the circuit are correct. However, you are looking at the tom most metal layer (with external pad connections. The actual circuitry corresponding to the RAM cell are at or just above Si substrate (Poly Si gates, and W-contacts to Metal-1 (W or Al). If this is 1995 vintage RAM, there will be about 6 transistors (0.35-0.5um gate width) making the circuit for the basic bit of storage (inverter circuit). To uncover that layout you will need to de-process the device (remove the top 3-5 metal layers), then image Metal-1, contact, and poly layers. Then draw the diagrams that are described by Fly-logic people.
The Anthlon64 information, is complete on the design part of the device, however they do not describe the basic RAM cell. The device has actually 3 areas of SRAM caches (L0, L1, and L2-largest array at top of the shown die) associated with the core. Also the Athlon64 is made on SOI, with poly gates at 0.09um or smaller and with Cu dual-damasceene metallization layers (9 of them). Remarkably enough the basic RAM cell layout is probably the same ... I used to help make those little things at AMD.
Have Fun, this is not a complicated work, but requires a lot of patience at the polishing wheel, chemical etching or RIE, and low KV FEG-SEM imaging.
Jerzy *************************************************** Jerzy Gazda Ph.D. Section Manager - TEM, FIB, SEM Cerium Laboratories LLC 5204 E. Ben White Blvd. MS-512 Austin, TX 78741
(512) 934-5185 vm (512) 622-6600 pgr
www.ceriumlabs.com
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I'm shooting electron micrographs of mostly planar structures on an IC which I assume is some kind of RAM. Initially I started looking at microprocessors, and, on-line I had found a number of diagrams that labeled areas of the microprocessor as SRAM, DRAM, ALU, etc. I switched to RAM assuming the same information would be readily available for RAM, i.e. some type of equivalent lay-out of various RAM chips at different magnifications but cannot find anything like the microprocessor diagrams:
Ditto light microscopy along the lines of fly logic, a reverse engineering firm that etches and uncovers, then images the various layers of chips:
http://www.flylogic.net/blog/?p=32
I figured all I have to know is where's the input area/encoding/memory/decoding/output, but I didn't translate this into identifying these areas from circuitry.
The chip has the number 7025 on it, and its metal layers number is 105, and it was probably made in 1994 or 1995. In the SE image number 0002 the upper left, where the pads are, is the middle of the chip, and, the large main memory area of the chip is the dark area on the bottom central third of the micrograph.
Can anyone tell me any information about these micrographs? Or where I can find information about planar RAM circuitry at various magnifications? Or if I'm even asking the right questions? I put some micrographs on the web at:
http://www.djwatt.com/
I did not first adjust to web resolution, so, to look at them as micrographs, it might be better to just copy and paste in a word document as they appear awful large on the screen. They should paste as nice 4" high micrographs into some type of document.
Probably off-line responses are better.
Thanks, Kleo
KLeoPullin-at-pacbell.net
==============================Original Headers============================== 14, 21 -- From kleopullin-at-pacbell.net Sun May 3 19:44:59 2009 14, 21 -- Received: from web83408.mail.sp1.yahoo.com (web83408.mail.sp1.yahoo.com [69.147.64.56]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n440iweU009479 14, 21 -- for {Microscopy-at-microscopy.com} ; Sun, 3 May 2009 19:44:59 -0500 14, 21 -- Received: (qmail 24041 invoked by uid 60001); 4 May 2009 00:44:58 -0000 14, 21 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=pacbell.net; s=s1024; t=1241397898; bh=VK0hFu/6ztRFxM+U/877rwx/viqiImV435xv9gHsQx4=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To :MIME-Version:Content-Type; b=VnOz6r0lGsz3UMupMhceznL+Y0XtzdP3LNFWdXVnLWt5jCYpjS8zIy/AyN7BaQg+KXOFvt T2q0hXe8neOSCm45wgRwQynvFxKmsL9BIOrxa6O2zM5FKCELLLzqcS21E+53+2pA0TILkWDD idW3TIXbBIEObPq48Tgr+VrCkx5RM= 14, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 21 -- s=s1024; d=pacbell.net; 14, 21 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To :MIME-Version:Content-Type; 14, 21 -- b=xmV3ZapcGz43FoAJNS2lg/yGfEUbp1QmnBuz8qJpEWVdNrzgU0f+5KknWhTI0oHSy90zGo LfE6x804Blk93F8070gdPI+jh4pTGiAsk93Mv8GmLiYBYEm5YMzalZJo61U8k5S5JdfIyM/n XZ8klmCLxYgL/dM72a1atKfvQHtzw=; 14, 21 -- Message-ID: {492913.22296.qm-at-web83408.mail.sp1.yahoo.com} 14, 21 -- X-YMail-OSG: YBAwSoYVM1mOO_TCUkhz_xu83MqTadf0Rno0.DjkhVbE3zcTR98XfvHMj0N_grLTTuDSg565 3ELpXAego5g1m3YzUsA2WQ_.6.9M6rpBxtqULeFwAf965MoWAbgJ5IUcmSn6czfzVm7Vul3G tCMw_f9u10w1RHOe2Wpk21VdM_7yKvz.N0Lof.yGunGac8_te9ddjIBnNBZqMyTr2GQcz7CY L4YEEjcrvbi7dLi9y9Bzwbzwbt0lhon_ckX9s1jRG._suegreka48BUxn5ynrYI8I0xthQA2 4avylLl7K7wu2NEAlbuidnhMJS0H.Eugbe.G.eVtnQrnmwK5SrXZRfU- 14, 21 -- Received: from [69.226.103.38] by web83408.mail.sp1.yahoo.com via HTTP; Sun, 03 May 2009 17:44:58 PDT 14, 21 -- X-Mailer: YahooMailWebService/0.7.289.1 14, 21 -- Date: Sun, 3 May 2009 17:44:58 -0700 (PDT) 14, 21 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 14, 21 -- Reply-To: kleopullin-at-pacbell.net 14, 21 -- Subject: Planar structures on IC - RAM 14, 21 -- To: Microscopy-at-microscopy.com 14, 21 -- MIME-Version: 1.0 14, 21 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
==============================Original Headers============================== 23, 28 -- From Jerzy.Gazda-at-spansion.com Mon May 4 13:17:02 2009 23, 28 -- Received: from usausmgw02.spansion.com (usausmgw02.spansion.com [12.110.209.162]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44IH1D1020347 23, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 4 May 2009 13:17:01 -0500 23, 28 -- X-IronPort-AV: E=McAfee;i="5300,2777,5605"; a="3300501" 23, 28 -- Received: from usausexbh1.spansion.com ([10.248.26.58]) 23, 28 -- by usausmgw02.spansion.com with ESMTP; 04 May 2009 11:17:01 -0700 23, 28 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by USAUSEXBH1.spansion.com with Microsoft SMTPSVC(6.0.3790.3959); 23, 28 -- Mon, 4 May 2009 13:17:01 -0500 23, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 28 -- Content-class: urn:content-classes:message 23, 28 -- MIME-Version: 1.0 23, 28 -- Content-Type: text/plain; 23, 28 -- charset="us-ascii" 23, 28 -- Subject: RE: [Microscopy] Planar structures on IC - RAM 23, 28 -- Date: Mon, 4 May 2009 13:17:00 -0500 23, 28 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF158738730-at-USAUSEXMBPF1.spansion.com} 23, 28 -- In-Reply-To: {200905040054.n440sL8n021003-at-ns.microscopy.com} 23, 28 -- X-MS-Has-Attach: 23, 28 -- X-MS-TNEF-Correlator: 23, 28 -- Thread-Topic: [Microscopy] Planar structures on IC - RAM 23, 28 -- Thread-Index: AcnMUt1ilwotOqR2QgyQAEMKK+J6SgAj7k6g 23, 28 -- References: {200905040054.n440sL8n021003-at-ns.microscopy.com} 23, 28 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com} 23, 28 -- To: {kleopullin-at-pacbell.net} , {Microscopy-at-microscopy.com} 23, 28 -- X-OriginalArrivalTime: 04 May 2009 18:17:01.0610 (UTC) FILETIME=[853320A0:01C9CCE4] 23, 28 -- Content-Transfer-Encoding: 8bit 23, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44IH1D1020347 ==============================End of - Headers==============================
Does anyone know of a source for a metallurgical mount holder that will accommodate 40 mm diameter metallurgical mounts? Pella has them for 25, 32, 38, and 51 mm diameter metallurgical mounts, but not 40. It's for an SEM, and I need a 3.2 mm pin. Thanks,
Joe
==============================Original Headers============================== 5, 22 -- From kintzjd-at-rocketmail.com Mon May 4 14:31:57 2009 5, 22 -- Received: from web53311.mail.re2.yahoo.com (web53311.mail.re2.yahoo.com [206.190.49.101]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n44JVtM5006627 5, 22 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 14:31:56 -0500 5, 22 -- Received: (qmail 23425 invoked by uid 60001); 4 May 2009 19:31:54 -0000 5, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=rocketmail.com; s=s1024; t=1241465514; bh=02fxjLnazLVe+i25jlRdc++FVAIucAEJThCeDOP8qkU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=k6WKvD3bmii7vRDJgCABHxiovAQ9+d+yxsPVHEBP5keLeY5ewksAKX1mzAE6DHF0BtU+rWSKiEIu+F1Zd22oU0002fETP6Z0O0yuT+AQSUsvqBt1H0RFfzO30DNB+06r0UPQzZKrAKbzxjylTYx+iaO8+c7Jqjojzwae9Uobhn4= 5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 22 -- s=s1024; d=rocketmail.com; 5, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 22 -- b=YTaRUfTc2jhQPiXGROeYlWIhFDlOg2W7ZoUl1un5T8WBsH4DOOLxfJVwQ+zY/4xBgdXnkq2a1ZaXANWoFGdq71aasXbYLuB0idJl0JwnFVvdzv77XSqfybMY3liCaAMyWIcKvnWnLs7SKVpB3/oEFGaaGrRXyeQj8KzOUyemU6Q=; 5, 22 -- Message-ID: {379998.19725.qm-at-web53311.mail.re2.yahoo.com} 5, 22 -- X-YMail-OSG: rsXG50EVM1msF6nGZsaCN9iklXG.xcG89FZ.Sxux5PnOwaF4np1Gcf0QTVeHrxDmVLR_xIZ9F82dC47pWilYSlsWolz.J3.QGEjnz1tdUxC7RAzgYDHDmpAMaFMvhBb6Mw2NN4RlEuxD.1HVsv23TYsvmBJSro6MSeG2y36Qiij6ffn03CWau.nmhvD8tdTwMnTQitJfkmeLwG0qkC3T7HerqJWkDnDiGBK4OPd.ztqstRLxPEV7 5, 22 -- Received: from [24.149.204.114] by web53311.mail.re2.yahoo.com via HTTP; Mon, 04 May 2009 12:31:54 PDT 5, 22 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1 5, 22 -- Date: Mon, 4 May 2009 12:31:54 -0700 (PDT) 5, 22 -- From: Joseph Kintz {kintzjd-at-rocketmail.com} 5, 22 -- Subject: Need 40 mm diameter metallurgical mount holder for SEM 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=iso-8859-1 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44JVtM5006627 ==============================End of - Headers==============================
Joe, If all else fails, use double sided tape on a 1" stub. Unless you're planning a lot of work near 90 deg tilt, it should work.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kintzjd-at-rocketmail.com [mailto:kintzjd-at-rocketmail.com] Sent: Monday, May 04, 2009 3:35 PM To: kenconverse-at-qualityimages.biz
Does anyone know of a source for a metallurgical mount holder that will accommodate 40 mm diameter metallurgical mounts? Pella has them for 25, 32, 38, and 51 mm diameter metallurgical mounts, but not 40. It's for an SEM, and I need a 3.2 mm pin. Thanks,
Joe
==============================Original Headers============================== 5, 22 -- From kintzjd-at-rocketmail.com Mon May 4 14:31:57 2009 5, 22 -- Received: from web53311.mail.re2.yahoo.com (web53311.mail.re2.yahoo.com [206.190.49.101]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n44JVtM5006627 5, 22 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 14:31:56 -0500 5, 22 -- Received: (qmail 23425 invoked by uid 60001); 4 May 2009 19:31:54 -0000 5, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=rocketmail.com; s=s1024; t=1241465514; bh=02fxjLnazLVe+i25jlRdc++FVAIucAEJThCeDOP8qkU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version :Content-Type:Content-Transfer-Encoding; b=k6WKvD3bmii7vRDJgCABHxiovAQ9+d+yxsPVHEBP5keLeY5ewksAKX1mzAE6DHF0BtU+rWSKiE Iu+F1Zd22oU0002fETP6Z0O0yuT+AQSUsvqBt1H0RFfzO30DNB+06r0UPQzZKrAKbzxjylTYx+ia O8+c7Jqjojzwae9Uobhn4= 5, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 22 -- s=s1024; d=rocketmail.com; 5, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version :Content-Type:Content-Transfer-Encoding; 5, 22 -- b=YTaRUfTc2jhQPiXGROeYlWIhFDlOg2W7ZoUl1un5T8WBsH4DOOLxfJVwQ+zY/4xBgdXnkq2a1Z aXANWoFGdq71aasXbYLuB0idJl0JwnFVvdzv77XSqfybMY3liCaAMyWIcKvnWnLs7SKVpB3/oEFG aaGrRXyeQj8KzOUyemU6Q=; 5, 22 -- Message-ID: {379998.19725.qm-at-web53311.mail.re2.yahoo.com} 5, 22 -- X-YMail-OSG: rsXG50EVM1msF6nGZsaCN9iklXG.xcG89FZ.Sxux5PnOwaF4np1Gcf0QTVeHrxDmVLR_xIZ9F82d C47pWilYSlsWolz.J3.QGEjnz1tdUxC7RAzgYDHDmpAMaFMvhBb6Mw2NN4RlEuxD.1HVsv23TYsv mBJSro6MSeG2y36Qiij6ffn03CWau.nmhvD8tdTwMnTQitJfkmeLwG0qkC3T7HerqJWkDnDiGBK4 OPd.ztqstRLxPEV7 5, 22 -- Received: from [24.149.204.114] by web53311.mail.re2.yahoo.com via HTTP; Mon, 04 May 2009 12:31:54 PDT 5, 22 -- X-Mailer: YahooMailRC/1277.35 YahooMailWebService/0.7.289.1 5, 22 -- Date: Mon, 4 May 2009 12:31:54 -0700 (PDT) 5, 22 -- From: Joseph Kintz {kintzjd-at-rocketmail.com} 5, 22 -- Subject: Need 40 mm diameter metallurgical mount holder for SEM 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=iso-8859-1 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44JVtM5006627 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 25 -- From kenconverse-at-qualityimages.biz Mon May 4 14:48:13 2009 17, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44JmCnJ021183 17, 25 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 14:48:13 -0500 17, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com 17, 25 -- with ESMTP 17, 25 -- id {20090504194809481.RLKH19475-at-cdptpa-omta01.mail.rr.com} ; 17, 25 -- Mon, 4 May 2009 19:48:09 +0000 17, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 17, 25 -- To: {kintzjd-at-rocketmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 17, 25 -- Subject: RE: [Microscopy] Need 40 mm diameter metallurgical mount holder for SEM 17, 25 -- Date: Mon, 4 May 2009 15:48:03 -0400 17, 25 -- Message-ID: {A1A83CC8020544D5AD51097E292FCF7F-at-Ken} 17, 25 -- MIME-Version: 1.0 17, 25 -- Content-Type: text/plain; 17, 25 -- charset="iso-8859-1" 17, 25 -- X-Priority: 3 (Normal) 17, 25 -- X-MSMail-Priority: Normal 17, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 17, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 17, 25 -- In-Reply-To: {200905041935.n44JZPlS010643-at-ns.microscopy.com} 17, 25 -- Thread-Index: AcnM73ij3ViyKmg3Rp6+9DX/ZngioAAAVMRQ 17, 25 -- Importance: Normal 17, 25 -- Content-Transfer-Encoding: 8bit 17, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n44JmCnJ021183 ==============================End of - Headers==============================
Thanks for your interest. Based on the fairly limited information I have, the machine is performing as per its factory testing and specifications. So any wobble I am seeing in the beam, is most likely down to external fields.
Regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
On 1/5/09 12:16 AM, "Roseann Csencsits" {RCsencsits-at-lbl.gov} wrote:
} Hi David, } Has the manufacturer verified that all of their power supplies are } within spec? } Did performance prove better in the factory? } } Roseann Csencsits, PhD } Scientist in Charge - Donner TEM Facility } Lawrence Berkeley Lab 01-365 } 1 Cyclotron Road } Berkeley, CA 94720 } 510-486-4548 } } } } } } } } On Apr 30, 2009, at 6:41 AM, david.mitchell-at-emu.usyd.edu.au wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both david.mitchell-at-emu.usyd.edu.au as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: david.mitchell-at-emu.usyd.edu.au } } Name: David Mitchell } } } } Organization: EM Unit, University of Sydney } } } } Title-Subject: [Filtered] TEM: Magnetic Field Cancellation Systems } } } } Question: Dear All } } } } We are currently commissioning a new 200kV TEM/STEM with an in-column } } filter. The room only just met environmental specs with respect to } } magnetic field levels, and the new instrument does meets performance } } specs in terms of STEM resolution. However, I think it could be } } better. A fixed probe at 1 million times does show evidence of } } external magnetic field interference. These is some jitter in the } } 1-10Hz range of about 1nm amplitude, and there is much lower } } frequency drift over longer time frames of } 1 minute. We have } } measured AC fields in various planes, at typically 1mG or less, but } } up to 2.2mG in some instances. I have used a Lundgren field } } compensation system in a lab which was way out of spec (15mG), and so } } realise that they can make a huge difference in those situations. } } However, does anyone have experience with such systems in relatively } } low field environments such as I have? Is any potential improvement } } in STEM performance going to be worth the ca $50k investment? } } } } Thanks in advance, } } } } Dave Mitchell } } } } Login Host: 129.78.64.103 } } --------------------------------------------------------------------------- } } } } ==============================Original } } Headers============================== } } 9, 11 -- From zaluzec-at-microscopy.com Thu Apr 30 08:33:32 2009 } } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id n3UDXV58022708 } } 9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 30 Apr 2009 08:33:32 } } -0500 } } 9, 11 -- Mime-Version: 1.0 } } 9, 11 -- Message-Id: {p06240800c61f58f9a856-at-[206.69.208.22]} } } 9, 11 -- Date: Thu, 30 Apr 2009 08:33:30 -0500 } } 9, 11 -- To: microscopy-at-microscopy.com } } 9, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of } } MicroscopyListserver) } } 9, 11 -- Subject: viaWWW: TEM: Magnetic Field Cancellation Systems } } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; } } format="flowed" } } ==============================End of - } } Headers============================== }
==============================Original Headers============================== 13, 26 -- From david.mitchell-at-emu.usyd.edu.au Mon May 4 18:40:51 2009 13, 26 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au [129.78.64.146]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n44NeoRv016508 13, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 May 2009 18:40:51 -0500 13, 26 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1]) 13, 26 -- by localhost (Postfix) with SMTP id 70540103970; 13, 26 -- Tue, 5 May 2009 09:40:47 +1000 (EST) 13, 26 -- Received: from MAIL3.mcs.usyd.edu.au (pioneer.mcs.usyd.edu.au [172.17.185.19]) 13, 26 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id 0F35D103926; 13, 26 -- Tue, 5 May 2009 09:40:46 +1000 (EST) 13, 26 -- Received: from 172.17.185.136 ([172.17.185.136]) by MAIL3.mcs.usyd.edu.au ([172.17.185.109]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.134]) with Microsoft Exchange Server HTTP-DAV ; 13, 26 -- Mon, 4 May 2009 23:40:45 +0000 13, 26 -- User-Agent: Microsoft-Entourage/11.3.6.070618 13, 26 -- Date: Tue, 05 May 2009 09:40:45 +1000 13, 26 -- Subject: Re: [Microscopy] viaWWW: TEM: Magnetic Field Cancellation Systems 13, 26 -- From: David Mitchell {david.mitchell-at-emu.usyd.edu.au} 13, 26 -- To: Roseann Csencsits {RCsencsits-at-lbl.gov} , {Microscopy-at-Microscopy.Com} 13, 26 -- CC: {david.mitchell-at-emu.usyd.edu.au} 13, 26 -- Message-ID: {C625BA1D.9EF%david.mitchell-at-emu.usyd.edu.au} 13, 26 -- Thread-Topic: [Microscopy] viaWWW: TEM: Magnetic Field Cancellation Systems 13, 26 -- Thread-Index: AcnNEb5x/MdHrjkEEd62AAAjMrpsqg== 13, 26 -- In-Reply-To: {AB9D0E24-CA2B-4E39-8BB3-0364B32A95B1-at-lbl.gov} 13, 26 -- Mime-version: 1.0 13, 26 -- Content-type: text/plain; 13, 26 -- charset="US-ASCII" 13, 26 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
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Email: camiller-at-anatomy.iupui.edu Name: Caroline Miller
Organization: Indiana University
Title-Subject: [Filtered] Old EM film
Question: I have several boxes (100 sheets) of Kodak, 31/4 x 4in. 4489 EM film. It is quite old, 1988, but has been kept in the refrigerator. Will give away if you pay for shipping.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reznik-at-ict.uni-karlsruhe.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
A simple solution: buy one of the 38 mm holders from Pella, and have the inside diameter machined out to 40.5 mm - I believe the wall thickness is enough to accommodate doing this,
Wil Bigelow =============================
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-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 8, 20 -- From bigelow-at-umich.edu Mon May 4 19:02:34 2009 8, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4502YBH027239 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 4 May 2009 19:02:34 -0500 8, 20 -- Received: FROM [99.130.70.208] (adsl-99-130-70-208.dsl.sfldmi.sbcglobal.net [99.130.70.208]) 8, 20 -- By hellskitchen.mr.itd.umich.edu ID 49FF8218.A7B50.29524 ; 8, 20 -- Authuser bigelow; 8, 20 -- 4 May 2009 20:02:32 EDT 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06240801c62531a356b0-at-[99.130.70.208]} 8, 20 -- In-Reply-To: {200905041939.n44JdJEJ015836-at-ns.microscopy.com} 8, 20 -- References: {200905041939.n44JdJEJ015836-at-ns.microscopy.com} 8, 20 -- Date: Mon, 4 May 2009 20:02:27 -0400 8, 20 -- To: kintzjd-at-rocketmail.com, Microscopy Listserver {microscopy-at-microscopy.com} 8, 20 -- From: Wil Bigelow {bigelow-at-umich.edu} 8, 20 -- Subject: Re: [Microscopy] Need 40 mm diameter metallurgical mount holder 8, 20 -- for SEM 8, 20 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4502YBH027239 ==============================End of - Headers==============================
Is anyone out there using a metal strip or graphite method to fuse small (maybe 100mg or so) amounts of rock powder without flux into a glassy bead which can thenbe mounted, cut, and polished for EPMA or LA-ICP-MS analysis?
I'm hoping to convert my (t)rusty old Edwards 306 vacuum coater into such a device but I'm trying to avoid reinventing too many wheels.
I remember seeing such a method setup at UC Davis about ten years ago but I haven't had any luck following that one up.
We actually want to use LA-ICP-MS so it seems to us that a graphite boat setup would be better from a contamination viewpoint than a metal strip.
Any references, tips, experiences etc would be most welcome.
cheers Ritchie Sims Geology Auckland University Auckland New Zealand
==============================Original Headers============================== 9, 28 -- From r.sims-at-auckland.ac.nz Mon May 4 20:11:59 2009 9, 28 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n451BwFF014482 9, 28 -- for {microscopy-at-Microscopy.Com} ; Mon, 4 May 2009 20:11:58 -0500 9, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 3FED29D427 9, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 13:11:58 +1200 (NZST) 9, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 9, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 9, 28 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 9, 28 -- with ESMTP id Wo6IE5CZAqg5 for {microscopy-at-Microscopy.Com} ; 9, 28 -- Tue, 5 May 2009 13:11:58 +1200 (NZST) 9, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 9, 28 -- (using TLSv1 with cipher DES-CBC3-SHA (168/168 bits)) 9, 28 -- (No client certificate requested) 9, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 1C1829D426 9, 28 -- for {microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 13:11:58 +1200 (NZST) 9, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 9, 28 -- To: microscopy-at-Microscopy.Com 9, 28 -- Date: Tue, 05 May 2009 13:14:31 +1200 9, 28 -- MIME-Version: 1.0 9, 28 -- Subject: fluxless rock bead fusion method? 9, 28 -- Message-ID: {4A003BB7.4502.14AFD56-at-r.sims.auckland.ac.nz} 9, 28 -- Priority: normal 9, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 28 -- Content-type: text/plain; charset=US-ASCII 9, 28 -- Content-transfer-encoding: 7BIT 9, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
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Organization: Institute of cellular biology and pathology, 1st fac med, Charles Uni, Prague
Title-Subject: [Filtered] broken pin on Gatan 626 cryoholder
Question: Dear fellow microscopists,
do you know if a broken stage-positioning pin on the Gatan 626 cryoholder can be replaced without having to send it to Gatan? The pin seems to be just "pressed" into the bar, so maybe careful drilling....Unfortunately we lost the broken part of the pin.
Moly apertures will oxidize if not cleaned in an evaporator. With PT you can flame them, which is an advantage. MO tends to be more durable than PT which is an advantage of MO.
We use special cleaning methods for MO apertures during production but it's cost prohibitive to use these techniques unless you're making and producing many moly and platinum apertures each day.
I would suggest you try cleaning the strip in acetone in an ultrasonic which might help a little, but without access to an evaporator you won't get really good cleaning.
Disclaimer: Ladd Research is in the business of producing and selling apertures for use in electron microscopes and other applications.
At 07:59 PM 5/4/2009, you wrote:
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==============================Original Headers============================== 15, 27 -- From jd-at-laddresearch.com Tue May 5 14:43:22 2009 15, 27 -- Received: from anders.electric.net (anders.electric.net [72.35.23.15]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45JhMRB031005 15, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 14:43:22 -0500 15, 27 -- Received: from 1M1QXx-0001Oj-UQ by anders.electric.net with emc1-ok (Exim 4.69) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1M1QXx-0001Pd-Vb; Tue, 05 May 2009 12:43:21 -0700 15, 27 -- Received: by emcmailer; Tue, 05 May 2009 12:43:21 -0700 15, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 15, 27 -- by anders.electric.net with esmtps (TLSv1:AES256-SHA:256) 15, 27 -- (Exim 4.69) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1M1QXx-0001Oj-UQ; Tue, 05 May 2009 12:43:21 -0700 15, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 27 -- Date: Tue, 05 May 2009 15:43:18 -0400 15, 27 -- To: reznik-at-ict.uni-karlsruhe.de 15, 27 -- From: jd {jd-at-laddresearch.com} 15, 27 -- Subject: Re: [Microscopy] viaWWW: Aperture_Cleaning 15, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 15, 27 -- In-Reply-To: {200905042359.n44Nx3XC016254-at-ns.microscopy.com} 15, 27 -- References: {200905042359.n44Nx3XC016254-at-ns.microscopy.com} 15, 27 -- Mime-Version: 1.0 15, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 27 -- X-Outbound-IP: 216.204.198.170 15, 27 -- X-Env-From: jd-at-laddresearch.com 15, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 15, 27 -- Message-Id: {E1M1QXx-0001Pd-Vb-at-anders.electric.net} ==============================End of - Headers==============================
Boris, A very simple way to clean almost all types of apertures (except thin foil gold self cleaning) is to polish them with 1µ diamond paste.
I prefer a cut-knap polishing cloth, but even a woven lint-free cloth will do. The 1µ diamond compound should be silicone-free (SPI meets this requirement, according to the late Chuck Garber, although many or most metallographic pastes have silicones). Place some diamond compound on the polishing cloth, place the aperture (or aperture strip) on the diamond, put your finger on it and start moving it in circles. When the first side is clean, flip it over and polish the other side. Ultrasonicate in hot water and Joy dishwashing liquid, rinse (tap water will suffice, although distilled is better) and blow dry with a duster or very clean compressed air, so there are no water spots.
Apertures can last decades. I've even taken apertures that had been overheated in an evaporator and didn't work, and made them whole again. Evaporator cleaning causes the metal to change its crystal structure and often after about 5-6 cleanings, the aperture is no good any more. Polishing will smear any distinct grain boundaries and make the aperture good again.
Have fun.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: reznik-at-ict.uni-karlsruhe.de [mailto:reznik-at-ict.uni-karlsruhe.de] Sent: Monday, May 04, 2009 7:56 PM To: kenconverse-at-qualityimages.biz
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what about using Pol? then, rinse with IPA and then methanol. I've done this for Wenhelts but not for apertures. I just replaced them with new Pt discs but have not had an aperture strip.
The aperture holder also got Pol and cleaning. The blue cast is cleaned off and stig returns to normal.
gary g.
At 03:52 PM 5/5/2009, you wrote:
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==============================Original Headers============================== 11, 22 -- From gary-at-gaugler.com Tue May 5 18:19:46 2009 11, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n45NJjMI003835 11, 22 -- for {microscopy-at-microscopy.com} ; Tue, 5 May 2009 18:19:46 -0500 11, 22 -- Message-Id: {200905052319.n45NJjMI003835-at-ns.microscopy.com} 11, 22 -- Received: (qmail 25367 invoked from network); 5 May 2009 16:32:35 -0700 11, 22 -- Received: by simscan 1.1.0 ppid: 25361, pid: 25365, t: 0.1482s 11, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 22 -- by smtp1 with SMTP; 5 May 2009 16:32:35 -0700 11, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 22 -- Date: Tue, 05 May 2009 16:19:43 -0700 11, 22 -- To: kenconverse-at-qualityimages.biz 11, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 22 -- Subject: Re: [Microscopy] RE: viaWWW: Aperture_Cleaning 11, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 22 -- In-Reply-To: {200905052252.n45Mqtlt023694-at-ns.microscopy.com} 11, 22 -- References: {200905052252.n45Mqtlt023694-at-ns.microscopy.com} 11, 22 -- Mime-Version: 1.0 11, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n45NJjMI003835 ==============================End of - Headers==============================
Biological Specimen Preparation Specialist/Microscopist Australian Key Centre for Microscopy and Microanalysis The University of Sydney Reference No. 154769
The Australian Key Centre for Microscopy & Microanalysis (AKCMM) is the headquarters of the Australian Microscopy and Microanalysis Research Facility (AMMRF), a major collaboration between government and universities. The Centre recognises the substantial role that microscopy, imaging and microanalysis are set to play in the next decade, as biotechnology and nanoscience have increasing impact on science and society. The Centre provides leadership, innovation and ingenuity in Australian science and engineering research. For more information, please visit www.emu.usyd.edu.au
The AKCMM seeks to appoint a highly-organised and self-driven individual to manage and operate Biological Specimen Preparation facilities and laboratory, and train and assist users with microscopy techniques and specimen imaging. You will have the opportunity to support a wide range of cutting-edge research, on our suite of state-of-the-art microscopy equipment. This position will see you provide training and assistance in various specimen preparation techniques, including but not be limited to chemical and cryo fixation. So expertise in these techniques is desirable. You will also be required to demonstrate microscopy techniques such as SEM, TEM, Confocal, and fluorescence microscopy, plus IT skills including databases and image and data processing software. Competency in some of these areas is required. Occasional national/international travel is also required.
An important part of the role is contribution towards preparation and delivery of training courses, coursework programs and workshops, and ensuring a productive and pleasant learning and working environment for all users. Therefore you must also be able to demonstrate strong interpersonal and communication skills. You will be an organised person who manages your priorities well. As this position also requires you to maintain documentation, manuals and instrument logs, as well as report usage trends and user/specimen information to management. You will also be responsible on ensuring safe equipment operation and the availability of adequate laboratory and instrument consumables.
To succeed, you will possess a relevant degree and a background in biology, biochemistry, chemistry, chemical or mechanical engineering, materials science or engineering, medicine, nanotechnology, pharmacy or physics.
If you are passionate about sharing your microscopy knowledge and supporting research, this is the job for you. The position is full-time fixed term for three years, subject to the completion of a satisfactory probation period for new appointees. Visa sponsorship and relocation assistance may be offered to suitable overseas applicants. A wide range of employment benefits are also available, including Living Away From Home Allowance (LAFHA).
Level of appointment and responsibility will be commensurate with qualifications and experience:
Remuneration package Level 5: $63k - $70k p.a. (which includes base salary $52k - $59k p.a., leave loading and 17% employer's contribution to superannuation).
Remuneration package Level 6: $72k - $78k p.a. (which includes base salary $61k - $66k p.a., leave loading and 17% employer's contribution to superannuation).
For more information and to apply, please visit http://positions.edu.edu.au and search by reference number 154769.
Specific enquiries about the role can be directed to Ellie Kable on (02) 9351 7566.
Closing Date: 21 May 2009
Kind regards,
Paulina Czerny Sydney Recruitment University of Sydney p.czerny-at-usyd.edu.au
==============================Original Headers============================== 18, 17 -- From p.czerny-at-usyd.edu.au Tue May 5 18:23:19 2009 18, 17 -- Received: from hrx.com.au (210-193-133-2.macquarie.net.au [210.193.133.2] (may be forged)) 18, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45NNHaA011369 18, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 18:23:18 -0500 18, 17 -- Received: from HRXSYD022 ([192.168.15.152]) by mail.hrx.com.au with InterScan Message Security Suite; Wed, 06 May 2009 09:26:47 +1000 18, 17 -- From: "Paulina Czerny" {p.czerny-at-usyd.edu.au} 18, 17 -- To: {Microscopy-at-Microscopy.Com} 18, 17 -- Subject: Biological Specimen Preparation Specialist/Microscopist 18, 17 -- Date: Wed, 6 May 2009 09:23:13 +1000 18, 17 -- Message-ID: {001001c9cdd8$75f859d0$980fa8c0-at-hrx.com.au} 18, 17 -- MIME-Version: 1.0 18, 17 -- Content-Type: text/plain; 18, 17 -- charset="us-ascii" 18, 17 -- Content-Transfer-Encoding: 7bit 18, 17 -- X-Mailer: Microsoft Office Outlook 11 18, 17 -- Thread-Index: AcnN2HXLpdEWa+tVQji2xZqZVcfJUw== 18, 17 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Careers in Microscopy and Microanalysis Expressions of Interest in Technical and Research Opportunities Reference No. 154930
The Australian Key Centre for Microscopy & Microanalysis (AKCMM), located in Camperdown, on the main campus of the University of Sydney, was established as the Electron Microscope Unit in 1958 and is a centralised corporate unit of the University. The Centre recognises the substantial role that microscopy, imaging and microanalysis are set to play in the next decade, as biotechnology and nanoscience have increasing impact on science and society. The Centre provides leadership, innovation and ingenuity in Australian science and engineering research and is seeking to recruit outstanding academic and general staff to advance this aim.
The Centre is a research leader and has some of the most advanced facilities in the world for microscopy and microanalysis. It serves as national headquarters for the Australian Microscopy and Microanalysis Research Facility (AMMRF) (www.ammrf.org.au). The Centre supports a diverse user base to develop techniques to enable understanding of the relationships between the structure and function of materials and living systems. For details see: www.emu.usyd.edu.au
There will soon be employment opportunities in the Centre in the form of technical staff positions and research staff positions. The positions are available at various levels and in various discipline areas. The principal role of the Centre's technical staff is to provide user-support. This takes the form variously of user-training: both one-on-one and in groups and via the Centre's coursework programs. User-support may also involve instrument and/or laboratory coordination and requires technical mastery of particular microscopy platforms. Direct work with users to help and enable them to make the most from the superb instrumentation in this outstanding Centre is a key job-function. We welcome Expressions of Interest (EoI) from scientists and engineers with interest, knowledge and expertise in TEM, SEM, FIB, light/laser microscopy and the microanalysis variants of these platforms.
Alternatively, our research staff positions are for early career researchers seeking postdoctoral research fellowship roles. Currently, the emerging positions in the Centre are grant-supported and mainly in the area of materials science and engineering. Passionate researchers with a materials science or relevant Ph.D. that have interest and knowledge in phase transformations and structure-property relationships and expertise in advanced electron microscopy and related techniques are encouraged to submit an EoI.
For more information and to apply, please visit http://positions.usyd.edu.au and search by reference number 154930.
Specific enquiries can be directed to Centre Manager Karen Hill via karen.hill-at-emu.usyd.edu.au or +61 2 9351 4499.
Close Date: 24 May 2009
Kind regards,
Paulina Czerny Sydney Recruitment University of Sydney p.czerny-at-usyd.edu.au
==============================Original Headers============================== 15, 17 -- From p.czerny-at-usyd.edu.au Tue May 5 18:25:14 2009 15, 17 -- Received: from hrx.com.au (210-193-133-2.macquarie.net.au [210.193.133.2] (may be forged)) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n45NPDEK017397 15, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 May 2009 18:25:13 -0500 15, 17 -- Received: from HRXSYD022 ([192.168.15.152]) by mail.hrx.com.au with InterScan Message Security Suite; Wed, 06 May 2009 09:28:43 +1000 15, 17 -- From: "Paulina Czerny" {p.czerny-at-usyd.edu.au} 15, 17 -- To: {Microscopy-at-Microscopy.Com} 15, 17 -- Subject: Careers in Microscopy and Microanalysis 15, 17 -- Date: Wed, 6 May 2009 09:25:08 +1000 15, 17 -- Message-ID: {001101c9cdd8$baf83af0$980fa8c0-at-hrx.com.au} 15, 17 -- MIME-Version: 1.0 15, 17 -- Content-Type: text/plain; 15, 17 -- charset="us-ascii" 15, 17 -- Content-Transfer-Encoding: 7bit 15, 17 -- X-Mailer: Microsoft Office Outlook 11 15, 17 -- Thread-Index: AcnN2Lq1BmIixakdSbCHjUpo95+/wg== 15, 17 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
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Email: RWNordhausen-at-UCDavis.edu Name: Robert W. Nordhausen
Organization: California Animal Health and Food Safety Lab
Title-Subject: [Filtered] TEM morphodiagnostic job opening
Question: Microscopists, Stand-alone TEM electron microcopist, thin section and negative stain morphodiagnostics in a food animal veterinary diagnostic lab. Experienced in ultrastructural pathology. PhD preferred. Requisition #0300622. https://www.employment.ucdavis.edu Thanks, Bob Nordhausen EM Lab California Animal Health and Food Safety Lab School of Veterinary Medicine University of California, Davis
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Email: zhangyc-at-gmail.com Name: Yucheng Zhang
Organization: university of cambridge
Title-Subject: [Filtered] CBEM pattern refining program
Question: I would like to do some nano-scale strain measurement using CBED. In order to retrieve the precise lattice parameters, experimental pattern is going to be compared with simulated pattern obtained using JEMS. I learn that a kai-square minimization process can be used to compare the experiemental data with simulation. Can anyone kindly suggest any free programs I can download to do the kai-square miminization for this purpose? Many thanks
I am seeking input regarding the usefulness/application of Field Emission SEM's in the field of Geology. If you are using one for geological applications, are you sing it for SEI imaging and/or Backscatter imaging? How about Cathodoluminescence? EDS? EBSD?
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 8, 28 -- From mmcheath-at-syr.edu Wed May 6 06:52:33 2009 8, 28 -- Received: from mx1.syr.edu (mx1.syr.edu [128.230.1.71]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46BqWsh013987 8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 06:52:33 -0500 8, 28 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 28 -- by mx1.syr.edu (8.14.3/8.14.3) with ESMTP id n46BqVoh021699 8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 07:52:31 -0400 8, 28 -- Received: from suex07-mbx-02.ad.syr.edu ([fe80::2403:4941:8a06:15cc]) by 8, 28 -- suex07-hub-01.ad.syr.edu ([2002:80e6:6cc3::80e6:6cc3]) with mapi; Wed, 6 May 8, 28 -- 2009 07:52:31 -0400 8, 28 -- From: Michael M Cheatham {mmcheath-at-syr.edu} 8, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 28 -- Date: Wed, 6 May 2009 07:52:30 -0400 8, 28 -- Subject: Field Emission SEM's: applications in the field of Geology 8, 28 -- Thread-Topic: Field Emission SEM's: applications in the field of Geology 8, 28 -- Thread-Index: AcnOQSJG2dnijbbIIEC8wI9U0F2AmA== 8, 28 -- Message-ID: {C626F23E.2D8B7%mmcheath-at-syr.edu} 8, 28 -- Accept-Language: en, en-US 8, 28 -- Content-Language: en 8, 28 -- X-MS-Has-Attach: 8, 28 -- X-MS-TNEF-Correlator: 8, 28 -- acceptlanguage: en, en-US 8, 28 -- Content-Type: text/plain; charset="iso-8859-1" 8, 28 -- MIME-Version: 1.0 8, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2009-05-06_06:2009-04-28,2009-05-06,2009-05-06 signatures=0 8, 28 -- X-Proofpoint-Spam-Reason: safe 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n46BqWsh013987 ==============================End of - Headers==============================
Highly hygroscopic tissues swell when sections are floated on water, and when they dry down to the slide, they have folds because they have stretched more than the surrounding tissue. I have tried floating the sections on some organic solvents instead of water, but the sections sink and end up even more wrinkled. Has anyone worked out a solution to this problem? I often have it with both "Epon" and ImmunoBed sections.
Ralph Common Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Wed May 6 11:11:11 2009 4, 24 -- Received: from sys06.mail.msu.edu (sys06.mail.msu.edu [35.9.75.106]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46GBB76009619 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 6 May 2009 11:11:11 -0500 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys06.mail.msu.edu with esmtpsa (Exim 4.63 #12) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1M1jiA-00024E-ON 4, 24 -- for Microscopy-at-microscopy.com; Wed, 06 May 2009 12:11:10 -0400 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Flat sections 4, 24 -- Date: Wed, 6 May 2009 13:10:46 -0400 4, 24 -- Message-ID: {000d01c9ce6d$9972bb40$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- Importance: Normal 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Hi Michael, Our CAMCOR nano-characterization facility is multi-disciplinary however, the geologists here at UofO find our suite of FEG SEMs (Zeiss Ultra 55, FEI Helios FIB and Quanta) extremely useful for all sorts of applications that hadn't imagined at the time of the instrument purchases.
For example, geologists are using a Quanta SEM to examine nano-scale decompression crystallization in eruptive materials and finding a host of interesting processes using both SE, BSE and even cathodoluminescence. In a related field, archeologists are taking advantage of our Titan TEM to characterize nano-diamonds from the Younger Dryas ice-age impact layer. Whoever heard of archeologists using a TEM, but taking advantage of new "eyes" is partly how new discoveries are made in science.
The question almost seems unnecessary as the geologists don't seem to stop their investigations at 50 or 100Kx any more than any other field. There certainly are useful things that can continue to be done at lower magnifications, but the capability for looking closer is always welcome and we wouldn't go back to tungsten filaments if I can help it.
The question is somewhat related to asking why would someone put a FEG on a microprobe or ESEM. My response would be, that while one can come up with a number of specialized applications that actually do benefit from performing x-ray analyses or low vacuum imaging on sub-micron domains, my basic answer is that it is simply a wonderfully powerful ability to be able to drop into low current or high vacuum mode and acquire a high resolution SE image with a click of the mouse without moving to another instrument.
That said, it is true that CL and EBSD tend to have inherently larger interaction volumes than SE or straight up BSE, but still the smaller spot sizes of a FEG don't hurt in these specific cases.
Another big advantage for FEG electron instruments is that they can operate with more stability at lower voltages than instruments with tungsten filaments sources. Being able to easily work below 5 keV has all sorts of analytical and imaging advantages for geologists as you might imagine. john
At 04:58 AM 5/6/2009, mmcheath-at-syr.edu wrote:
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==============================Original Headers============================== 11, 21 -- From donovan-at-uoregon.edu Wed May 6 11:32:03 2009 11, 21 -- Received: from QMTA12.emeryville.ca.mail.comcast.net (qmta12.emeryville.ca.mail.comcast.net [76.96.27.227]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46GW2g3024565 11, 21 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 11:32:02 -0500 11, 21 -- Message-Id: {200905061632.n46GW2g3024565-at-ns.microscopy.com} 11, 21 -- Received: from OMTA01.emeryville.ca.mail.comcast.net ([76.96.30.11]) 11, 21 -- by QMTA12.emeryville.ca.mail.comcast.net with comcast 11, 21 -- id oBdc1b0020EPchoACGY3Vf; Wed, 06 May 2009 16:32:03 +0000 11, 21 -- Received: from SOURCE.uoregon.edu ([71.237.220.159]) 11, 21 -- by OMTA01.emeryville.ca.mail.comcast.net with comcast 11, 21 -- id oGY21b00Q3SwvZT8MGY22u; Wed, 06 May 2009 16:32:03 +0000 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Wed, 06 May 2009 09:32:00 -0700 11, 21 -- To: microscopy-at-microscopy.com 11, 21 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 11, 21 -- Subject: Re: [Microscopy] Field Emission SEM's: applications in the 11, 21 -- field of Geology 11, 21 -- In-Reply-To: {200905061158.n46BwddQ022036-at-ns.microscopy.com} 11, 21 -- References: {200905061158.n46BwddQ022036-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
On May 6, 2009, at 9:11 AM, rcommon-at-msu.edu wrote:
} Highly hygroscopic tissues swell when sections are floated on water, } and } when they dry down to the slide, they have folds because they have } stretched } more than the surrounding tissue. I have tried floating the } sections on } some organic solvents instead of water, but the sections sink and } end up } even more wrinkled. Has anyone worked out a solution to this } problem? I } often have it with both "Epon" and ImmunoBed sections.
Dear Ralph, I have had no experience with this problem, so I don't know if this will work, but the sections should not sink in solvents that are heavier than water and have surface tension greater than water. I think there are several such solvents; I know the chlorinated ones-- CCl4, CHCl3, etc--are heavier than water, but I don't know what their surface tensions are, and I do not know whether they will damage the sections, since they are pretty active solvents. I think that n- butanol might have sufficiently high surface tension, and it would definitely be gentler on the sections, but it may not have sufficient density. Possibly some mixture could be found that has all the right properties. Good luck. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed May 6 12:42:35 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46HgZ7f009641 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 12:42:35 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 5738766E16A3 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 10:42:35 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id A059066E4185 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 10:42:33 -0700 (PDT) 6, 22 -- Message-Id: {61649146-36EE-4141-BFB6-4030173AD2FD-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200905061611.n46GBKtm009745-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] Flat sections 6, 22 -- Date: Wed, 6 May 2009 10:42:33 -0700 6, 22 -- References: {200905061611.n46GBKtm009745-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Thanks for those who responded to my original post. I was not specific enough about the problem, and how I normally handle semithin sections. I float the 1 micron sections on drops of water on slides, and heat the slides on a hotplate until they have dried down. For most tissues I get perfectly flat sections that adhere well. The problem is differential stretching of the section, with some areas stretching more than the surrounding areas of the section. This is a problem particularly with lichen and other ascomycete fruiting bodies. Most of the section lies flat, but the hymenium, the area of most interest, has multiple folds. The folds are present even before staining. The hymenium contains gel-like polysaccharides that absorb large amounts of water, causing excessive swelling and stretching of the hymenial areas relative to the surrounding tissue, and thus folds in the hymenium when the sections dry down. My thought was to float the sections on an organic solvent instead of water, but the sections just sink in the solvents I have tried so far. Has anyone had success floating sections on something other than water? Any other cure for the problem? Cryosectioning avoids the problem, but I would like to be able to get good sections with embedded specimens.
Ralph Common Michigan State University
==============================Original Headers============================== 3, 24 -- From rcommon-at-msu.edu Wed May 6 12:46:13 2009 3, 24 -- Received: from sys03.mail.msu.edu (sys03.mail.msu.edu [35.9.75.103]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n46HkBCw014387 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 6 May 2009 12:46:12 -0500 3, 24 -- Received: from [35.9.122.125] (helo=emlab) 3, 24 -- by sys03.mail.msu.edu with esmtpsa (Exim 4.63 #12) 3, 24 -- (TLSv1:RC4-MD5:128) 3, 24 -- id 1M1lC7-000474-Nz 3, 24 -- for Microscopy-at-microscopy.com; Wed, 06 May 2009 13:46:11 -0400 3, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 3, 24 -- To: {Microscopy-at-microscopy.com} 3, 24 -- Subject: Flat sections (again) 3, 24 -- Date: Wed, 6 May 2009 14:45:47 -0400 3, 24 -- Message-ID: {001401c9ce7a$df7b46e0$7d7a0923-at-msu.edu} 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="iso-8859-1" 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 (Normal) 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 3, 24 -- Importance: Normal 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933 3, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
I've dropped the S-5700 in favor of the older S-570 as a general purpose SEM that is low cost of maintenance.
Can any of you tell me about the good, the bad, the ugly side of this system? It can have a turbo or DP. I am only looking for LaB6 capability. Depending on what EDS it might have, digital capture is possible. Otherwise, I would look at Quartz PCI. At some point, I would put an EDAX Apollo 10 on this system.
I know very little about Hitachi and JEOL but much about Amray and Zeiss. Thus, I seek input from the collective.
Off-line is OK.
gary g.
==============================Original Headers============================== 7, 17 -- From gary-at-gaugler.com Wed May 6 19:52:43 2009 7, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n470qh99026708 7, 17 -- for {microscopy-at-microscopy.com} ; Wed, 6 May 2009 19:52:43 -0500 7, 17 -- Message-Id: {200905070052.n470qh99026708-at-ns.microscopy.com} 7, 17 -- Received: (qmail 10200 invoked from network); 6 May 2009 18:05:36 -0700 7, 17 -- Received: by simscan 1.1.0 ppid: 10197, pid: 10198, t: 0.1400s 7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 17 -- by smtp1 with SMTP; 6 May 2009 18:05:36 -0700 7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 17 -- Date: Wed, 06 May 2009 17:52:35 -0700 7, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 7, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 17 -- Subject: Hitachi S-570 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I recently discovered (in storage) Reichart/Leica KF80 Plunge Freezer and AFS (Automatic Freeze Substitution) systems. I would like to get these up and running but the manuals have vanished into the mists of time.
I have tried to find the manuals on the 'net with no success (yet).
If anyone has copies (electronic preferably) they could send it would be very much appreciated.
Thank you very much.
Colin Veitch Electron Microscopist CSIRO Materials Science and Engineering, Geelong Laboratory PO Box 21, BELMONT, Vic. 3216. Australia. colin.veitch-at-csiro.au
The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.
To the knowing, Putting an Amray ECO-SEM into operation, that was moved and did not run for several years, the attached EDX-Detector turned out to have a defective window. It is an Oxford Link Pentafet that was formerly used for Gun Shot Residue analysis. Has anyone suggestions where to let the detector repair reasonably (in Europe preferably but not exclusively)? There are rumours that it is possible to recondition the dewar instead of replacing it in such a case. Is that recommendable and who will make that? Will the SiLi or efferent electronics be affected by the damage? Any (almost) comment greatly welcomed.
Regards Gerd biolab for environmental analysis Braunschweig, Germany
ps. a bit depressed as after promising start first the BSE, but now the EDX detector as well turned out to be defective.
==============================Original Headers============================== 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 5, 19 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.177]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47FB79D004161 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de [89.183.66.151]) 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis) 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} 5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200 5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: SEM EDX window and dewar repair - service resp. 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx ==============================End of - Headers==============================
They have been involved wtih Reichert Histology for decades.
Good hunting Barbara Foster
Barbara Foster, President and Sr. Consultant
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now scheduling customized, on-site courses through September. Call me for a free assessment and quote.
At 01:38 AM 5/7/2009, Colin.Veitch-at-csiro.au wrote:
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==============================Original Headers============================== 12, 23 -- From bfostermme-at-sbcglobal.net Thu May 7 10:48:26 2009 12, 23 -- Received: from smtp114.sbc.mail.mud.yahoo.com (smtp114.sbc.mail.mud.yahoo.com [68.142.198.213]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n47FmO6a019969 12, 23 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:48:25 -0500 12, 23 -- Message-Id: {200905071548.n47FmO6a019969-at-ns.microscopy.com} 12, 23 -- Received: (qmail 54225 invoked from network); 7 May 2009 15:48:22 -0000 12, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 23 -- s=s1024; d=sbcglobal.net; 12, 23 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:In-Reply-To:References:Mime-Version:Content-Type; 12, 23 -- b=JADJDhcz62jbzrDdikqina0Si0oDEhA0NiX4Vi1UUnXd2sTsnVHJR2N6m5o7TbH8ZcBv270EVfd+ZNpuzBeHVa2CBwx1frW5/PHdq9sTSQv9Qdh/e1lihub5F84C6zIAeI2ux4kiEb6dyFO/QNXEnqQF4W1mpFSNbVbjqhVFXX0= ; 12, 23 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login) 12, 23 -- by smtp114.sbc.mail.mud.yahoo.com with SMTP; 7 May 2009 15:48:22 -0000 12, 23 -- X-YMail-OSG: gIBYdpYVM1k5yxbGvgaeyCbsnVsy55s2_wr5xXfY2Hxnd6BnmldPwXGTYm.jb24EAcHe4Q4MFZCFsKwWs_EJ.yMccUrIjUgCmBC1JJzJBlpCuD0XzK.5N2KLuWhngbkBnPG6wbtelUM5du5UU1JcX3twqOC4ccPH_Eo3w9SwDRRXtosmb66kAuTYOchshxTKGcmTGuiv8K2u1YqzNZ53azipt8.TBk_lh1e1ksaf.d0aAgNBSYqmVwGPQ5SrMgt9fFIt8FIIZ5cTVCVimCzl_VFrLVjGDVf7amJAb7oZtRoKGFCUqVZAOvaOfPXVHI8saD76rv4iSm4J.3sPDIqWawg- 12, 23 -- X-Yahoo-Newman-Property: ymail-3 12, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 23 -- Date: Thu, 07 May 2009 10:42:24 -0500 12, 23 -- To: Colin.Veitch-at-csiro.au, microscopy-at-microscopy.com 12, 23 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 12, 23 -- Subject: Re: [Microscopy] Manuals for Reichart/Leica KF80 and AFS 12, 23 -- In-Reply-To: {200905070637.n476bf50006119-at-ns.microscopy.com} 12, 23 -- References: {200905070637.n476bf50006119-at-ns.microscopy.com} 12, 23 -- Mime-Version: 1.0 12, 23 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Many thanks to all those who replied to my call for help with sections that dry down folded in hygroscopic areas of the tissue, such as the hymenium of many ascomycetes. Most of the replies were off-line, so I will summarize the suggestions in case they are of interest to others on the list.
Some people thought my tissue was poorly dehydrated or poorly infiltrated. I have processed thousands of samples of other tissues without problems, so I'm confident that poor dehydration isn't the issue. When the thick gelatinous cell wall polysaccharide layer is dehydrated, either by air drying or in alcohol, the tissue becomes extremely dense. Unlike cells, where the cytoplasmic water is replaced by ethanol, then resin, the gel of the hymenium becomes almost solid polysaccharide, and there is little space left for resin. When the sections are floated on water, the polysaccharides rehydrate and expand. Some people suggested that I use GMA or another water soluble resin. I have tried ImmunoBed and JB-4, and the results are the same. I even tried a crude freeze-substitution (hydrated apothecium frozen and placed in JB-4 at -20 degrees for a month). Infiltration was poor and I still had folds.
Several people suggested alternative fluids to float the sections. These included CCl4, CHCl3, polyethylene glycol, n-butanol, oil, water with high salt concentration, and water-solvent mixtures. A practical alternative to water would have to have be safe, dry without residue, float the section and let it stretch evenly and attach firmly to the slide. So far the solvents I have tried wet both surfaces of the section and the solvents swell the resin unevenly, resulting in even more wrinkles.
Techniques to improved stretching of the sections as they dry down was suggested by several people. These included extending the warming period by adding water to the droplet on the slide, or by using xylene or chloroform vapor in addition to heating. One ingenious technique involves inverting the slide over xylene until the droplet evaporates. Others suggested drying down the sections slowly. The people who mentioned hotplate temperatures suggested 45-60 degrees. I have always dried down my sections at just short of boiling. This works great for "normal" tissue, giving nice flat sections that adhere well. Perhaps the lower temperature will help with my ascomycetes.
A published technique for getting flat Epon sections of 4-10 microns thickness was cited:
Preparation and flattening of thick epoxy sections for light microscopy. Thompson D. Pizzolato. Can.J.Bot. 54 (20); p2405-2407.1976.
More often people suggested cutting the sections thinner, trimming blocks as tightly as possible, and notching the periphery of the resin to release pressure.
I haven't had a chance to try most of these suggestions yet. If any prove successful, I will let the group know.
Ralph Common Michigan State University
==============================Original Headers============================== 13, 24 -- From rcommon-at-msu.edu Thu May 7 11:54:05 2009 13, 24 -- Received: from sys53.mail.msu.edu (sys53.mail.msu.edu [35.9.75.233]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47Gs5bJ005168 13, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 11:54:05 -0500 13, 24 -- Received: from [35.9.122.125] (helo=emlab) 13, 24 -- by sys53.mail.msu.edu with esmtpsa (Exim 4.63 #12) 13, 24 -- (TLSv1:RC4-MD5:128) 13, 24 -- id 1M26rD-0002bY-QF 13, 24 -- for Microscopy-at-microscopy.com; Thu, 07 May 2009 12:54:03 -0400 13, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 13, 24 -- To: {Microscopy-at-microscopy.com} 13, 24 -- Subject: Flat sections (summary) 13, 24 -- Date: Thu, 7 May 2009 13:53:39 -0400 13, 24 -- Message-ID: {002001c9cf3c$c15bdc20$7d7a0923-at-msu.edu} 13, 24 -- MIME-Version: 1.0 13, 24 -- Content-Type: text/plain; 13, 24 -- charset="iso-8859-1" 13, 24 -- Content-Transfer-Encoding: 7bit 13, 24 -- X-Priority: 3 (Normal) 13, 24 -- X-MSMail-Priority: Normal 13, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 13, 24 -- Importance: Normal 13, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933 13, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Gerd, Yes, the dewar can be reconditioned (pumped) and anything else that might have been damaged when the window went can also be repaired. Although he's not in Europe, my customers have had good luck with Jim Nicolino in Florida.
Jim Nicolino AAT 1816 St Johns Bluff Road Suite 305 Jacksonville, Florida 32246 (904) 646-3069 (904) 646-3131 fax JNicolino-at-advancedanalysistech.com http://www.AdvancedAnalysisTech.com
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: gerd-at-biolab.de [mailto:gerd-at-biolab.de] Sent: Thursday, May 07, 2009 11:17 AM To: kenconverse-at-qualityimages.biz
To the knowing, Putting an Amray ECO-SEM into operation, that was moved and did not run for several years, the attached EDX-Detector turned out to have a defective window. It is an Oxford Link Pentafet that was formerly used for Gun Shot Residue analysis. Has anyone suggestions where to let the detector repair reasonably (in Europe preferably but not exclusively)? There are rumours that it is possible to recondition the dewar instead of replacing it in such a case. Is that recommendable and who will make that? Will the SiLi or efferent electronics be affected by the damage? Any (almost) comment greatly welcomed.
Regards Gerd biolab for environmental analysis Braunschweig, Germany
ps. a bit depressed as after promising start first the BSE, but now the EDX detector as well turned out to be defective.
==============================Original Headers============================== 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 5, 19 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.177]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47FB79D004161 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de [89.183.66.151]) 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis) 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} 5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200 5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: SEM EDX window and dewar repair - service resp. 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx ==============================End of - Headers==============================
==============================Original Headers============================== 18, 25 -- From kenconverse-at-qualityimages.biz Thu May 7 13:42:37 2009 18, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47IgYmY025359 18, 25 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 13:42:36 -0500 18, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta04.mail.rr.com 18, 25 -- with ESMTP 18, 25 -- id {20090507184232799.NMSF13493-at-cdptpa-omta04.mail.rr.com} ; 18, 25 -- Thu, 7 May 2009 18:42:32 +0000 18, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 25 -- To: {gerd-at-biolab.de} , "MSA Listserver" {microscopy-at-microscopy.com} 18, 25 -- Subject: RE: [Microscopy] SEM EDX window and dewar repair - service resp. 18, 25 -- Date: Thu, 7 May 2009 14:42:23 -0400 18, 25 -- Message-ID: {62B60FD54B05484EB82C4868B0D6A56E-at-Ken} 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Type: text/plain; 18, 25 -- charset="us-ascii" 18, 25 -- X-Priority: 3 (Normal) 18, 25 -- X-MSMail-Priority: Normal 18, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 18, 25 -- In-Reply-To: {200905071517.n47FHC92008323-at-ns.microscopy.com} 18, 25 -- Importance: Normal 18, 25 -- Thread-Index: AcnPJu0Us2YFr5R0Qq61idwxPCzsYQAG+bOw 18, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n47IgYmY025359 ==============================End of - Headers==============================
He is an EDS specialist doing repair on windows, pumping dewars etc. Just a satisfied customer.
Yours, Stefan Diller
gerd-at-biolab.de schrieb: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } To the knowing, } Putting an Amray ECO-SEM into operation, that was moved and did not run } for several years, the attached EDX-Detector turned out to have a } defective window. It is an Oxford Link Pentafet that was formerly used } for Gun Shot Residue analysis. Has anyone suggestions where to let the } detector repair reasonably (in Europe preferably but not exclusively)? } There are rumours that it is possible to recondition the dewar instead } of replacing it in such a case. Is that recommendable and who will make } that? } Will the SiLi or efferent electronics be affected by the damage? } Any (almost) comment greatly welcomed. } } Regards } Gerd } biolab for environmental analysis } Braunschweig, Germany } } ps. a bit depressed as after promising start first the BSE, but now the } EDX detector as well turned out to be defective. } } } } ==============================Original Headers============================== } 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 } 5, 19 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.126.177]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47FB79D004161 } 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500 } 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de [89.183.66.151]) } 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis) } 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200 } 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} } 5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200 } 5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de} } 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- To: Microscopy-at-microscopy.com } 5, 19 -- Subject: SEM EDX window and dewar repair - service resp. } 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 19 -- Content-Transfer-Encoding: 7bit } 5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59 } 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO } 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx } ==============================End of - Headers============================== }
-- ----------------------------------------------------- Stefan Diller - Wissenschaftliche Photographie Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
My message today is motivated by questions asked by a quality systems auditor who recently visited my lab.
We calibrate the XYZ scan axes of our AFM using a traceable height and pitch standard made of thermally grown silicon dioxide on silicon. The specimen contains these patterns:
10 um pitch, 2-dimensional array of pits, nominally 200 nm deep
2 um pitch, 1-dimensional array of ridges, same depth as the pits.
The manufacturer's certificate of traceability does not discuss recalibration or indicate any need for it.
Based on the materials of construction, I believe that the pitch values and the pit depth are stable and that no recalibration is needed, provided that we are careful in handling and using the specimen. For example, any nanodebris visible in the image is excluded from the measurement.
However, the quality consultant says that it is common to send such samples to an outside lab for recalibration. He added that we should either do recalibration or write a justification for why recalibration is not necessary.
I am curious what other people do about this. One can consider this in several different environments:
-the AFM or SEM manufacturer, who wants to provide good calibration for the instruments
-the AFM or SEM user who is supporting a production or QC application
-the AFM or SEM user who is mainly supporting R&D, but needs to show traceability in order to meet the requirements of a quality management system.
Here are a few practical questions to start the discussion.
If you have such a standard recalibrated, what is the time interval?
If you do not recalibrate the standard, what rationale do you give for why it is unnecessary?
Can you articulate the reasons so that it is clear they are based on good science and engineering data and practices?
Do you have any other comments on this subject?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 28, 24 -- From donc-at-asmicro.com Thu May 7 15:00:09 2009 28, 24 -- Received: from smtp107.sbc.mail.re2.yahoo.com (smtp107.sbc.mail.re2.yahoo.com [68.142.229.98]) 28, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n47K083K024768 28, 24 -- for {microscopy-at-microscopy.com} ; Thu, 7 May 2009 15:00:08 -0500 28, 24 -- Received: (qmail 26126 invoked from network); 7 May 2009 20:00:07 -0000 28, 24 -- Received: from unknown (HELO asm15) (donc-at-76.252.13.152 with login) 28, 24 -- by smtp107.sbc.mail.re2.yahoo.com with SMTP; 7 May 2009 20:00:06 -0000 28, 24 -- X-YMail-OSG: fZcAt_sVM1lsgrq9MF2qfzW.vMZG5.iYsStX5U1x93tUxd.JHrJEs_7vA1ZtmxMQA4MWFzhH2VQv21ZPWg3.kmzepUFSSrjvirWqiMAwJZePR4xZ4T0RWCnXBXM7SfUUplmGQNA4kAxcNPp3WAWPbw9YskCenLVT2vPSqLu8F2NHijlgw5GZEewdSDniOWtX6cyb_r34qDwlY.rHV.SePToqH_zna6c4WyvCZuFDZzlpg9XNJqYwajxNpiPuoZou.I4gbJYoXaIer.FGJfarStBxAS9GyExqkjlJeVbEn_U5cECnzzNCp9isdVZBjn4WU1Z_yWcihTRE6Y_BzzXQAiI5wu6TKN7nwnC_ 28, 24 -- X-Yahoo-Newman-Property: ymail-3 28, 24 -- Message-ID: {FDB45F447CC049829760E9C1386EF7F4-at-asm15} 28, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 28, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 28, 24 -- Subject: Recalibration of AFM and SEM Calibration Standards 28, 24 -- Date: Thu, 7 May 2009 15:59:13 -0400 28, 24 -- MIME-Version: 1.0 28, 24 -- Content-Type: text/plain; 28, 24 -- format=flowed; 28, 24 -- charset="iso-8859-1"; 28, 24 -- reply-type=original 28, 24 -- Content-Transfer-Encoding: 7bit 28, 24 -- X-Priority: 3 28, 24 -- X-MSMail-Priority: Normal 28, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 28, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
From cxzeriqqrbpxts5qof8kr-at-washington.edu Thu May 7 15:04:41 2009 Return-Path: {cxzeriqqrbpxts5qof8kr-at-washington.edu} Received: from google.com (122-121-240-221.dynamic.hinet.net [122.121.240.221]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n47K4efb031284 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 7 May 2009 15:04:41 -0500 Received: from [190.102.27.161] (HELO google.com) by cold-girlie.de; Fri, 8 May 2009 04:04:25 +0800
I have an associate who needs PIXE equipment. Do any of you have any suggestions for vendors of such an instrument? Also, how about an agent or rep for such instrument in Hong Kong area?
Thanks
Don Kloos VP Sales, Marketing, Business Development Parallax Research, Inc.
Thank you so much for the responses to my request for the manuals for the above instruments. I now have the manuals for both from a variety of sources!
All I have to do now is try and find all the bits and put them together!
Cheers from a slightly damp Geelong - rain is still a bit of a novelty here!!
Colin Veitch Electron Microscopist CSIRO Materials Science and Engineering, Geelong Laboratory PO Box 21, BELMONT, Vic. 3216. Australia. colin.veitch-at-csiro.au
The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.
I have a eumeX EDS detector, made in Germany, www.eumeX.com, and I am very satisfied with it. They manufacture detectors, and will also repair and upgrade old ones.
Gresham, in the UK, used to do these also but I think perhaps they don't after having been taken over by ev2.
Oxford would be the obvious people to ask first, as I think their Pentafet detectors may be tricky to interface to other preamps (something about needing an externally generated reset pulse?), but are they still in the EDS business since they were taken over by Gattan?
If you're buying a new one, check out the experiences of others, as some brands are reputed to have dewars more likely to lose their vacuum than others.
good luck
Ritchie Sims Geology University of Auckland Auckland, New Zealand
Quoting gerd-at-biolab.de:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } To the knowing, } Putting an Amray ECO-SEM into operation, that was moved and did not run } for several years, the attached EDX-Detector turned out to have a } defective window. It is an Oxford Link Pentafet that was formerly used } for Gun Shot Residue analysis. Has anyone suggestions where to let the } detector repair reasonably (in Europe preferably but not exclusively)? } There are rumours that it is possible to recondition the dewar instead } of replacing it in such a case. Is that recommendable and who will make } that? } Will the SiLi or efferent electronics be affected by the damage? } Any (almost) comment greatly welcomed. } } Regards } Gerd } biolab for environmental analysis } Braunschweig, Germany } } ps. a bit depressed as after promising start first the BSE, but now the } EDX detector as well turned out to be defective. } } } } ==============================Original Headers============================== } 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 } 5, 19 -- Received: from moutng.kundenserver.de } (moutng.kundenserver.de [212.227.126.177]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n47FB79D004161 } 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500 } 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de } [89.183.66.151]) } 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis) } 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200 } 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} } 5, 19 -- Date: Thu, 07 May 2009 17:11:05 +0200 } 5, 19 -- From: Gerd Mueller von der Haegen {gerd-at-biolab.de} } 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) } 5, 19 -- MIME-Version: 1.0 } 5, 19 -- To: Microscopy-at-microscopy.com } 5, 19 -- Subject: SEM EDX window and dewar repair - service resp. } 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 19 -- Content-Transfer-Encoding: 7bit } 5, 19 -- X-Provags-ID: V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59 } 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO } 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx } ==============================End of - Headers============================== }
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==============================Original Headers============================== 15, 41 -- From r.sims-at-auckland.ac.nz Fri May 8 02:55:14 2009 15, 41 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n487tD71004433 15, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 02:55:14 -0500 15, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 568B466916C; 15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST) 15, 41 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 15, 41 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 15, 41 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 41 -- with ESMTP id 9CQIf4N4HMCZ; Fri, 8 May 2009 19:55:08 +1200 (NZST) 15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz (uxchorprd01.its.auckland.ac.nz [130.216.10.20]) 15, 41 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 15, 41 -- (No client certificate requested) 15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 26BE166916B; 15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST) 15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz (localhost.localdomain [127.0.0.1]) 15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8) with ESMTP id n487t71L025540; 15, 41 -- Fri, 8 May 2009 19:55:07 +1200 15, 41 -- Received: (from apache-at-localhost) 15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8/Submit) id n487t5GT025539; 15, 41 -- Fri, 8 May 2009 19:55:05 +1200 15, 41 -- X-Authentication-Warning: uxchorprd01.its.auckland.ac.nz: apache set sender to r.sims-at-auckland.ac.nz using -f 15, 41 -- Received: from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz 15, 41 -- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with HTTP; 15, 41 -- Fri, 08 May 2009 19:55:05 +1200 15, 41 -- Message-ID: {20090508195505.46401850sghpdh7k-at-webmail.auckland.ac.nz} 15, 41 -- Date: Fri, 08 May 2009 19:55:05 +1200 15, 41 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz} 15, 41 -- To: Microscopy-at-microscopy.com 15, 41 -- Subject: Re: [Microscopy] SEM EDX window and dewar repair - service resp. 15, 41 -- References: {200905071513.n47FDGK5005559-at-ns.microscopy.com} 15, 41 -- In-Reply-To: {200905071513.n47FDGK5005559-at-ns.microscopy.com} 15, 41 -- MIME-Version: 1.0 15, 41 -- Content-Type: text/plain; 15, 41 -- charset=ISO-8859-1; 15, 41 -- DelSp="Yes"; 15, 41 -- format="flowed" 15, 41 -- Content-Disposition: inline 15, 41 -- Content-Transfer-Encoding: 7bit 15, 41 -- User-Agent: Internet Messaging Program (IMP) 4.3.3 ==============================End of - Headers==============================
Just to set the record straight, Gresham Scientific Instruments Ltd was acquired in July 2005 by e2v technologies plc and subsequently changed its name to e2v scientific instruments Ltd in March 2006.
E2v scientific instruments Ltd has not changed its business profile in any way and continues to offer a high quality worldwide EDX detector repair service in addition to its manufactured products.
Kind regards Peter
______________ Dr Peter Smith Sales Director
e2v scientific instruments ltd Sirius House, Watery Lane Wooburn Green, High Wycombe, Bucks, HP10 0AP, UK
This communication is for the exclusive use of the intended recipient(s) and contains information that is confidential and may also be privileged. If you are not the intended recipient(s) please note that any form of distribution, copying or use of this communication, the information in it or in attachments is strictly prohibited and may be unlawful. If you have received this communication in error please return it to the sender, and then delete all copies of the email and its attachments. Registered office: 106 Waterhouse Lane, Chelmsford, Essex, CM1 2QU England. Company reg. no. 344963. VAT no. GB669490581.
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: 08 May 2009 09:07 To: Smith, Peter
Hi Gerd
I have a eumeX EDS detector, made in Germany, www.eumeX.com, and I am very satisfied with it. They manufacture detectors, and will also repair and upgrade old ones.
Gresham, in the UK, used to do these also but I think perhaps they don't after having been taken over by ev2.
Oxford would be the obvious people to ask first, as I think their Pentafet detectors may be tricky to interface to other preamps (something about needing an externally generated reset pulse?), but are they still in the EDS business since they were taken over by Gattan?
If you're buying a new one, check out the experiences of others, as some brands are reputed to have dewars more likely to lose their vacuum than others.
good luck
Ritchie Sims Geology University of Auckland Auckland, New Zealand
Quoting gerd-at-biolab.de:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } To the knowing, } Putting an Amray ECO-SEM into operation, that was moved and did not } run for several years, the attached EDX-Detector turned out to have a } defective window. It is an Oxford Link Pentafet that was formerly used
} for Gun Shot Residue analysis. Has anyone suggestions where to let the
} detector repair reasonably (in Europe preferably but not exclusively)? } There are rumours that it is possible to recondition the dewar instead
} of replacing it in such a case. Is that recommendable and who will } make that? } Will the SiLi or efferent electronics be affected by the damage? } Any (almost) comment greatly welcomed. } } Regards } Gerd } biolab for environmental analysis } Braunschweig, Germany } } ps. a bit depressed as after promising start first the BSE, but now } the EDX detector as well turned out to be defective. } } } } ==============================Original } Headers============================== } 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 5, 19 -- } Received: from moutng.kundenserver.de (moutng.kundenserver.de } [212.227.126.177]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n47FB79D004161 } 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500 } 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de } [89.183.66.151]) } 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis) } 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200 } 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} 5, 19 -- Date: Thu, } 07 May 2009 17:11:05 +0200 5, 19 -- From: Gerd Mueller von der Haegen } {gerd-at-biolab.de} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 } (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To: } Microscopy-at-microscopy.com 5, 19 -- Subject: SEM EDX window and dewar } repair - service resp. } 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Provags-ID: } V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59 } 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO } 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 36, 33 -- From Peter.Smith-at-e2v.com Fri May 8 03:32:20 2009 36, 33 -- Received: from mail195.messagelabs.com (mail195.messagelabs.com [85.158.138.147]) 36, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n488WIBH019943 36, 33 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 03:32:19 -0500 36, 33 -- X-VirusChecked: Checked 36, 33 -- X-Env-Sender: Peter.Smith-at-e2v.com 36, 33 -- X-Msg-Ref: server-13.tower-195.messagelabs.com!1241771538!13955364!1 36, 33 -- X-StarScan-Version: 6.0.0; banners=e2v.com,-,- 36, 33 -- X-Originating-IP: [213.48.15.234] 36, 33 -- Received: (qmail 3315 invoked from network); 8 May 2009 08:32:18 -0000 36, 33 -- Received: from host.e2v.com (HELO WHL32.e2v.com) (213.48.15.234) 36, 33 -- by server-13.tower-195.messagelabs.com with SMTP; 8 May 2009 08:32:18 -0000 36, 33 -- Received: from WHL46.e2v.com ([162.1.51.83]) by WHL32.e2v.com with Microsoft SMTPSVC(6.0.3790.1830); 36, 33 -- Fri, 8 May 2009 09:32:18 +0100 36, 33 -- Received: from WYCOM3.e2v.com ([162.1.11.6]) by WHL46.e2v.com with Microsoft SMTPSVC(6.0.3790.1830); 36, 33 -- Fri, 8 May 2009 09:32:17 +0100 36, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 36, 33 -- Content-class: urn:content-classes:message 36, 33 -- MIME-Version: 1.0 36, 33 -- Content-Type: text/plain; 36, 33 -- charset="us-ascii" 36, 33 -- Subject: [Microscopy] Re: SEM EDX window and dewar repair - service resp. 36, 33 -- Date: Fri, 8 May 2009 09:32:16 +0100 36, 33 -- Message-ID: {112184A2B7A9204CBE5F3B4DB06C4DFF6646E4-at-WYCOM3.e2v.com} 36, 33 -- X-MS-Has-Attach: 36, 33 -- X-MS-TNEF-Correlator: 36, 33 -- Thread-Topic: [Microscopy] Re: SEM EDX window and dewar repair - service resp. 36, 33 -- thread-index: AcnPs+s4rGdPmxUQRaKJjMuIvkaNhAAAijQg 36, 33 -- From: "Smith, Peter" {Peter.Smith-at-e2v.com} 36, 33 -- To: {microscopy-at-microscopy.com} 36, 33 -- X-OriginalArrivalTime: 08 May 2009 08:32:17.0707 (UTC) FILETIME=[7F375FB0:01C9CFB7] 36, 33 -- Content-Transfer-Encoding: 8bit 36, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n488WIBH019943 ==============================End of - Headers==============================
Oxford Instruments still handle EDX. Their cryo business was taken over by Gatan.
Dave
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: 08 May 2009 09:00 To: David Patton
Hi Gerd
I have a eumeX EDS detector, made in Germany, www.eumeX.com, and I am very satisfied with it. They manufacture detectors, and will also repair and upgrade old ones.
Gresham, in the UK, used to do these also but I think perhaps they don't after having been taken over by ev2.
Oxford would be the obvious people to ask first, as I think their Pentafet detectors may be tricky to interface to other preamps (something about needing an externally generated reset pulse?), but are they still in the EDS business since they were taken over by Gattan?
If you're buying a new one, check out the experiences of others, as some brands are reputed to have dewars more likely to lose their vacuum than others.
good luck
Ritchie Sims Geology University of Auckland Auckland, New Zealand
Quoting gerd-at-biolab.de:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society
} of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } To the knowing, } Putting an Amray ECO-SEM into operation, that was moved and did not } run for several years, the attached EDX-Detector turned out to have a } defective window. It is an Oxford Link Pentafet that was formerly used
} for Gun Shot Residue analysis. Has anyone suggestions where to let the
} detector repair reasonably (in Europe preferably but not exclusively)? } There are rumours that it is possible to recondition the dewar instead
} of replacing it in such a case. Is that recommendable and who will } make that? } Will the SiLi or efferent electronics be affected by the damage? } Any (almost) comment greatly welcomed. } } Regards } Gerd } biolab for environmental analysis } Braunschweig, Germany } } ps. a bit depressed as after promising start first the BSE, but now } the EDX detector as well turned out to be defective. } } } } ==============================Original } Headers============================== } 5, 19 -- From gerd-at-biolab.de Thu May 7 10:11:08 2009 5, 19 -- } Received: from moutng.kundenserver.de (moutng.kundenserver.de } [212.227.126.177]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n47FB79D004161 } 5, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 May 2009 10:11:08 -0500 } 5, 19 -- Received: from [192.168.0.104] (a89-183-66-151.net-htp.de } [89.183.66.151]) } 5, 19 -- by mrelayeu.kundenserver.de (node=mrelayeu3) with ESMTP (Nemesis) } 5, 19 -- id 0MKxQS-1M25FZ1rfO-0002Mc; Thu, 07 May 2009 17:11:06 +0200 } 5, 19 -- Message-ID: {4A02FA09.5000907-at-biolab.de} 5, 19 -- Date: Thu, } 07 May 2009 17:11:05 +0200 5, 19 -- From: Gerd Mueller von der Haegen } {gerd-at-biolab.de} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 } (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To: } Microscopy-at-microscopy.com 5, 19 -- Subject: SEM EDX window and dewar } repair - service resp. } 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- X-Provags-ID: } V01U2FsdGVkX1/SGD6cKm4x9tFhj594hlkv8VZeDen582kDo59 } 5, 19 -- u3kitZ6xMgIDzt+I5kC/0Ey3Ns/LNumcJYkhIBEwoEkt4RwMgO } 5, 19 -- JjghOspqSmpI8H74fFMgX8swtAh9nZx } ==============================End of - } Headers============================== }
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==============================Original Headers============================== 15, 41 -- From r.sims-at-auckland.ac.nz Fri May 8 02:55:14 2009 15, 41 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 15, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n487tD71004433 15, 41 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 02:55:14 -0500 15, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 568B466916C; 15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST) 15, 41 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 15, 41 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 15, 41 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 41 -- with ESMTP id 9CQIf4N4HMCZ; Fri, 8 May 2009 19:55:08 +1200 (NZST) 15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz (uxchorprd01.its.auckland.ac.nz [130.216.10.20]) 15, 41 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 15, 41 -- (No client certificate requested) 15, 41 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 26BE166916B; 15, 41 -- Fri, 8 May 2009 19:55:08 +1200 (NZST) 15, 41 -- Received: from uxchorprd01.its.auckland.ac.nz (localhost.localdomain [127.0.0.1]) 15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8) with ESMTP id n487t71L025540; 15, 41 -- Fri, 8 May 2009 19:55:07 +1200 15, 41 -- Received: (from apache-at-localhost) 15, 41 -- by uxchorprd01.its.auckland.ac.nz (8.13.8/8.13.8/Submit) id n487t5GT025539; 15, 41 -- Fri, 8 May 2009 19:55:05 +1200 15, 41 -- X-Authentication-Warning: uxchorprd01.its.auckland.ac.nz: apache set sender to r.sims-at-auckland.ac.nz using -f 15, 41 -- Received: from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz 15, 41 -- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with HTTP; 15, 41 -- Fri, 08 May 2009 19:55:05 +1200 15, 41 -- Message-ID: {20090508195505.46401850sghpdh7k-at-webmail.auckland.ac.nz} 15, 41 -- Date: Fri, 08 May 2009 19:55:05 +1200 15, 41 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz} 15, 41 -- To: Microscopy-at-microscopy.com 15, 41 -- Subject: Re: [Microscopy] SEM EDX window and dewar repair - service resp. 15, 41 -- References: {200905071513.n47FDGK5005559-at-ns.microscopy.com} 15, 41 -- In-Reply-To: {200905071513.n47FDGK5005559-at-ns.microscopy.com} 15, 41 -- MIME-Version: 1.0 15, 41 -- Content-Type: text/plain; 15, 41 -- charset=ISO-8859-1; 15, 41 -- DelSp="Yes"; 15, 41 -- format="flowed" 15, 41 -- Content-Disposition: inline 15, 41 -- Content-Transfer-Encoding: 7bit 15, 41 -- User-Agent: Internet Messaging Program (IMP) 4.3.3 ==============================End of - Headers==============================
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==============================Original Headers============================== 31, 36 -- From David.Patton-at-uwe.ac.uk Fri May 8 04:39:08 2009 31, 36 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66]) 31, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n489d6PM004673 31, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 04:39:07 -0500 31, 36 -- Received: from (unknown [164.11.132.60]) by mailapp04.uwe.ac.uk with smtp 31, 36 -- id 412d_1013469e_3bb4_11de_8c18_00142223915c; 31, 36 -- Fri, 08 May 2009 10:39:04 +0100 31, 36 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 31, 36 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 31, 36 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 31, 36 -- 2005)) with SMTP id {0KJB002TIK4UJQ-at-mta01.uwe.ac.uk} for 31, 36 -- Microscopy-at-microscopy.com; Fri, 08 May 2009 10:38:56 +0100 (BST) 31, 36 -- Date: Fri, 08 May 2009 10:36:34 +0100 31, 36 -- From: David Patton {David.Patton-at-uwe.ac.uk} 31, 36 -- Subject: RE: [Microscopy] Re: SEM EDX window and dewar repair - service resp. 31, 36 -- re Oxford Instruments 31, 36 -- In-reply-to: {200905080800.n488014r010186-at-ns.microscopy.com} 31, 36 -- To: r.sims-at-auckland.ac.nz 31, 36 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF85203-at-egen-uwe01} 31, 36 -- MIME-version: 1.0 31, 36 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 31, 36 -- Content-type: text/plain; charset=US-ASCII 31, 36 -- Content-class: urn:content-classes:message 31, 36 -- Thread-topic: [Microscopy] Re: SEM EDX window and dewar repair - service resp. 31, 36 -- re Oxford Instruments 31, 36 -- Thread-index: AcnPswRJklZ0/o97T+SEq7iNrbUPBgADTcKw 31, 36 -- X-MS-Has-Attach: 31, 36 -- X-MS-TNEF-Correlator: 31, 36 -- References: {200905080800.n488014r010186-at-ns.microscopy.com} 31, 36 -- X-NAIMIME-Disclaimer: 1 31, 36 -- X-NAIMIME-Modified: 1 31, 36 -- X-NAI-Spam-Score: 0 31, 36 -- X-NAI-Spam-Rules: 1 Rules triggered 31, 36 -- RV3270=0 31, 36 -- Content-Transfer-Encoding: 8bit 31, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n489d6PM004673 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eawoodruff-at-live.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eawoodruff-at-live.com Name: Elvin Woodruff III
Organization: Tennessee State University
Title-Subject: [Filtered] Nitrogen leak
Question: Hi All,
I have recently started working with a JEOL 6701F scanning electron microscopy. The scope is running just fine, but it is going through nitrogen gas like there is not tomorrow. The scope sits on an air table which is supplied by gas (could this be it?)and other areas such as the specimen load chamber is also filled with nitrogen gas. I have talked to a couple of other people about the gas loss but to no avail. I have checked all of the usual places for a leak but can't find anything. We don't have a service contract right now so any help or ideas on why we are going through so much gas would be a big help.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reznik-at-ict.uni-karlsruhe.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reznik-at-ict.uni-karlsruhe.de Name: Reznik Boris
Have you checked the tube of the air table where nitrogen is vented when you bump into the scope or put something on the scope console? I had an air table for my ultra-microtome and at one time found that there was a small, noiseless stream of gas coming out of the small tube when there was no disturbance. The lever beside that tube which touches the bottom of the table was not going totally back to the closed/up position and needed a small bit of lubrication.
You mentioned that you have checked several places for the leak. Do you have tubing connecting the tank to your scope that is of the proper material to contain nitrogen? I do not recall the name/type of the tubing that I used but it was milky white in color, not very flexible and about 8mm in diameter. Someone on the List will most likely tell us the name.
Let us know when you do find out what is causing your gas to be used up so quickly.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced. ------------------------------------------------------------------------ X-from: {eawoodruff-at-live.com} Reply-To: {eawoodruff-at-live.com}
Who (re)calibrates these quality auditors? What certification do they have? It seems like this auditor is out of their element.
Maybe I am a little flip to think that the burden of proof should go the other way. We could assume that a standard would still be valid unless it has been demonstrated that it can go out of calibration over some period of time. Frankly, I cannot understand why someone would think a piece of silicon would change dimension on that scale because it sat on a shelf for 10 years. I think diffusion is a little slower than that.
Disclaimer: Our lab has come under such rigorous, externally supervised quality control. We use our experience to check the quality of our own work. I daresay we do as well, and maybe better, than some labs that pass the auditor's review.
Warren S.
-----Original Message----- X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com] Sent: Thursday, May 07, 2009 3:01 PM To: wesaia-at-iastate.edu
My message today is motivated by questions asked by a quality systems auditor who recently visited my lab.
We calibrate the XYZ scan axes of our AFM using a traceable height and pitch standard made of thermally grown silicon dioxide on silicon. The specimen contains these patterns:
10 um pitch, 2-dimensional array of pits, nominally 200 nm deep
2 um pitch, 1-dimensional array of ridges, same depth as the pits.
The manufacturer's certificate of traceability does not discuss recalibration or indicate any need for it.
Based on the materials of construction, I believe that the pitch values and the pit depth are stable and that no recalibration is needed, provided that we are careful in handling and using the specimen. For example, any nanodebris visible in the image is excluded from the measurement.
However, the quality consultant says that it is common to send such samples to an outside lab for recalibration. He added that we should either do recalibration or write a justification for why recalibration is not necessary.
I am curious what other people do about this. One can consider this in several different environments:
-the AFM or SEM manufacturer, who wants to provide good calibration for the instruments
-the AFM or SEM user who is supporting a production or QC application
-the AFM or SEM user who is mainly supporting R&D, but needs to show traceability in order to meet the requirements of a quality management system.
Here are a few practical questions to start the discussion.
If you have such a standard recalibrated, what is the time interval?
If you do not recalibrate the standard, what rationale do you give for why it is unnecessary?
Can you articulate the reasons so that it is clear they are based on good science and engineering data and practices?
Do you have any other comments on this subject?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 35, 37 -- From wesaia-at-iastate.edu Fri May 8 10:29:37 2009 35, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 35, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48FTZlq019909 35, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:29:36 -0500 35, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 35, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n48FTRwP013689; 35, 37 -- Fri, 8 May 2009 10:29:27 -0500 35, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 35, 37 -- id 3a8b_0486950c_3be5_11de_bbf4_00137253420a; 35, 37 -- Fri, 08 May 2009 10:29:27 -0500 35, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 35, 37 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n48FTQep022370; 35, 37 -- Fri, 8 May 2009 10:29:26 -0500 35, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 35, 37 -- Fri, 8 May 2009 10:29:26 -0500 35, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 35, 37 -- Content-class: urn:content-classes:message 35, 37 -- MIME-Version: 1.0 35, 37 -- Content-Type: text/plain; 35, 37 -- charset="us-ascii" 35, 37 -- Subject: RE: [Microscopy] Recalibration of AFM and SEM Calibration Standards 35, 37 -- Date: Fri, 8 May 2009 10:30:26 -0500 35, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE723E-at-maire.eng.iastate.edu} 35, 37 -- In-Reply-To: {200905072001.n47K11mR025823-at-ns.microscopy.com} 35, 37 -- X-MS-Has-Attach: 35, 37 -- X-MS-TNEF-Correlator: 35, 37 -- Thread-Topic: [Microscopy] Recalibration of AFM and SEM Calibration Standards 35, 37 -- Thread-Index: AcnPTo/GoVCN8eZBS/ePYCdGH9/lsQAm6MdQ 35, 37 -- References: {200905072001.n47K11mR025823-at-ns.microscopy.com} 35, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 35, 37 -- To: {Microscopy-at-microscopy.com} 35, 37 -- Cc: {donc-at-asmicro.com} 35, 37 -- X-OriginalArrivalTime: 08 May 2009 15:29:26.0780 (UTC) FILETIME=[C5B4D3C0:01C9CFF1] 35, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.8.151924 35, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0' 35, 37 -- Content-Transfer-Encoding: 8bit 35, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48FTZlq019909 ==============================End of - Headers==============================
No one has mentioned what quality system is being used for certification. My guess is it's an ISO system, but exactly which system it is has to be known. ISO 2001 and 2008 do not mandate a time frame for calibration. rather it is up to the company that has the equipment to determine what is a reasonable time frame for re-calibration. I'm not familiar with all other quality systems, but it would state in the standard how calibration has to be handeled.
I have scales for weight I have recalibrated every 4 months. I have gage blocks I re-calibrate every 3 years. I have optical calibration standards I never re-calibrate (essentially, I listed the calibration time period as 50 years, way more than I'll ever need to worry about). These later would be the equivalent to the standard orginally asked about. These are a non-contact calibration standard and were recalibrated once a number of years ago after having been in use for about 30 years. Since they were in calibration at the time of recalibration - the material (as in your case) does not degrade over time - the usage is non-contact - I have the historical record showing that over 3 decades they never lost calibration - I can then say they don't need re-calibration for another 50 years to the ISO auditor. They don't like it and have said to me that I should reconsider that and at least re-calibrate every 10 years. But, I am in compliance.
Essentially, it is the historical records that determine the time frame for re-calibration. Kind of a chicken and egg scenario, with the initial time frame chosen through best guess. Then if the standard is never out of calibration over a couple of re-calibrations, then you can justify widening the time period.
As an FYI, the auditors that I have dealt with in fact do get "recalibrated" ... :)
dj
On Fri, 8 May 2009, wesaia-at-iastate.edu wrote:
} Who (re)calibrates these quality auditors? What certification do they } have? It seems like this auditor is out of their element. } } Maybe I am a little flip to think that the burden of proof should go the } other way. We could assume that a standard would still be valid unless } it has been demonstrated that it can go out of calibration over some } period of time. Frankly, I cannot understand why someone would think a } piece of silicon would change dimension on that scale because it sat on } a shelf for 10 years. I think diffusion is a little slower than that. } } Disclaimer: Our lab has come under such rigorous, externally supervised } quality control. We use our experience to check the quality of our own } work. I daresay we do as well, and maybe better, than some labs that } pass the auditor's review. } } Warren S. } } -----Original Message----- } X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com] } Sent: Thursday, May 07, 2009 3:01 PM } To: wesaia-at-iastate.edu } Subject: [Microscopy] Recalibration of AFM and SEM Calibration Standards } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } My message today is motivated by questions asked by a quality systems } auditor who recently visited my lab. } } } } We calibrate the XYZ scan axes of our AFM using a traceable height and } pitch } standard made of thermally grown silicon dioxide on silicon. The } specimen } contains these patterns: } } 10 um pitch, 2-dimensional array of pits, nominally 200 nm deep } } 2 um pitch, 1-dimensional array of ridges, same depth as the pits. } } } } The manufacturer's certificate of traceability does not discuss } recalibration or indicate any need for it. } } Based on the materials of construction, I believe that the pitch values } and } the pit depth are stable and that no recalibration is needed, provided } that } we are careful in handling and using the specimen. For example, any } nanodebris visible in the image is excluded from the measurement. } } However, the quality consultant says that it is common to send such } samples } to an outside lab for recalibration. He added that we should either do } recalibration or write a justification for why recalibration is not } necessary. } } } } I am curious what other people do about this. One can consider this in } several different environments: } } -the AFM or SEM manufacturer, who wants to provide good calibration for } the } instruments } } -the AFM or SEM user who is supporting a production or QC application } } -the AFM or SEM user who is mainly supporting R&D, but needs to show } traceability in order to meet the requirements of a quality management } system. } } } } Here are a few practical questions to start the discussion. } } If you have such a standard recalibrated, what is the time interval? } } If you do not recalibrate the standard, what rationale do you give for } why } it is unnecessary? } } Can you articulate the reasons so that it is clear they are based on } good } science and engineering data and practices? } } Do you have any other comments on this subject? } } } } regards, } Don } ============================================= } Don Chernoff, Ph.D., President } Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com } 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 } INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & } Canada) } web: http://www.asmicro.com Fax: 317-895-5652 } [business activities: analytical services in AFM, AFM probes, } consulting, } training, } calibration and test specimens, calibration and measurement software, } used NanoScope equipment.] } ============================================= } } } } ==============================Original Headers============================== } 35, 37 -- From wesaia-at-iastate.edu Fri May 8 10:29:37 2009 } 35, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) } 35, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48FTZlq019909 } 35, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:29:36 -0500 } 35, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) } 35, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n48FTRwP013689; } 35, 37 -- Fri, 8 May 2009 10:29:27 -0500 } 35, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp } 35, 37 -- id 3a8b_0486950c_3be5_11de_bbf4_00137253420a; } 35, 37 -- Fri, 08 May 2009 10:29:27 -0500 } 35, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) } 35, 37 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n48FTQep022370; } 35, 37 -- Fri, 8 May 2009 10:29:26 -0500 } 35, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); } 35, 37 -- Fri, 8 May 2009 10:29:26 -0500 } 35, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 35, 37 -- Content-class: urn:content-classes:message } 35, 37 -- MIME-Version: 1.0 } 35, 37 -- Content-Type: text/plain; } 35, 37 -- charset="us-ascii" } 35, 37 -- Subject: RE: [Microscopy] Recalibration of AFM and SEM Calibration Standards } 35, 37 -- Date: Fri, 8 May 2009 10:30:26 -0500 } 35, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE723E-at-maire.eng.iastate.edu} } 35, 37 -- In-Reply-To: {200905072001.n47K11mR025823-at-ns.microscopy.com} } 35, 37 -- X-MS-Has-Attach: } 35, 37 -- X-MS-TNEF-Correlator: } 35, 37 -- Thread-Topic: [Microscopy] Recalibration of AFM and SEM Calibration Standards } 35, 37 -- Thread-Index: AcnPTo/GoVCN8eZBS/ePYCdGH9/lsQAm6MdQ } 35, 37 -- References: {200905072001.n47K11mR025823-at-ns.microscopy.com} } 35, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} } 35, 37 -- To: {Microscopy-at-microscopy.com} } 35, 37 -- Cc: {donc-at-asmicro.com} } 35, 37 -- X-OriginalArrivalTime: 08 May 2009 15:29:26.0780 (UTC) FILETIME=[C5B4D3C0:01C9CFF1] } 35, 37 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.8.151924 } 35, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0' } 35, 37 -- Content-Transfer-Encoding: 8bit } 35, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48FTZlq019909 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 19 -- From dljones-at-bestweb.net Fri May 8 10:54:01 2009 8, 19 -- Received: from smtp3.bestweb.net (smtp3.bestweb.net [209.94.103.43]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48Fs09A002783 8, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:54:00 -0500 8, 19 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202]) 8, 19 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 19 -- (No client certificate requested) 8, 19 -- by smtp3.bestweb.net (Postfix) with ESMTPS id 242E85CD3 8, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 11:53:59 -0400 (EDT) 8, 19 -- Date: Fri, 8 May 2009 15:19:49 +0000 (UTC) 8, 19 -- From: dljones {dljones-at-bestweb.net} 8, 19 -- To: microscopy-at-microscopy.com 8, 19 -- Subject: Re: [Microscopy] RE: Recalibration of AFM and SEM Calibration 8, 19 -- Standards 8, 19 -- In-Reply-To: {200905081533.n48FXN8j025242-at-ns.microscopy.com} 8, 19 -- Message-ID: {Pine.BSF.4.64.0905081502200.75218-at-monet.bestweb.net} 8, 19 -- References: {200905081533.n48FXN8j025242-at-ns.microscopy.com} 8, 19 -- MIME-Version: 1.0 8, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
On May 8, 2009, at 8:04 AM, connellyps-at-nhlbi.nih.gov wrote:
} I do not recall the name/type of the tubing } that I used but it was milky white in color, not very flexible and } about 8mm } in diameter. Someone on the List will most likely tell us the name.
Dear Pat, Two of the more likely possibilities are polyethylene and teflon. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri May 8 12:45:38 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48HjbOd022034 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 12:45:37 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 75C0832803E 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:45:37 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 6C343328081 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 10:45:36 -0700 (PDT) 6, 22 -- Message-Id: {6CA1162D-742F-4FB5-81B1-DC2504BD5763-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200905081504.n48F4SP0004749-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v930.3) 6, 22 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak 6, 22 -- Date: Fri, 8 May 2009 10:45:33 -0700 6, 22 -- References: {200905081504.n48F4SP0004749-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Elgin; My I suggest also that you have created a significant safety hazard by running your air table, and I presume your valving, on nitrogen. The leak you now have (and everything develops leaks eventually) is filling up your room with pure nitrogen and displacing the oxygen. You should be using only clean dry air for these items so that when it leaks, the room fills up with.....air! Air that you can breath! Nitrogen should only be used for venting the chamber.
Sent from my iPhone. John Mardinly
On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote:
} } } } --- } --- } ---------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --- } --- } ---------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --- } --- } --------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both eawoodruff-at-live.com as well as the MIcroscopy } Listserver } --- } --- } --------------------------------------------------------------------- } } Email: eawoodruff-at-live.com } Name: Elvin Woodruff III } } Organization: Tennessee State University } } Title-Subject: [Filtered] Nitrogen leak } } Question: Hi All, } } I have recently started working with a JEOL 6701F scanning electron } microscopy. The scope is running just fine, but it is going through } nitrogen gas like there is not tomorrow. The scope sits on an air } table which is supplied by gas (could this be it?)and other areas } such as the specimen load chamber is also filled with nitrogen gas. I } have talked to a couple of other people about the gas loss but to no } avail. I have checked all of the usual places for a leak but can't } find anything. We don't have a service contract right now so any help } or ideas on why we are going through so much gas would be a big help. } } Thanks } } Elvin } } Login Host: 68.19.196.245 } --- } --- } --------------------------------------------------------------------- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Fri May 8 07:41:46 2009 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22 } ]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n48CfjvE003327 } 9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 } 07:41:46 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c629d8f35379-at-[206.69.208.22]} } 9, 11 -- Date: Fri, 8 May 2009 07:41:44 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: eawoodruff-at-live.com (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Nitrogen leak } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
I would suspect polyethylene. It sounds a lot like the PEX (polyethylene cross-linked) pipe used in home domestic water applications. Teflon would probably be too flexible. It would be too expensive for the thickness required to stand the pressure.
Warren S.
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Friday, May 08, 2009 12:47 PM To: wesaia-at-iastate.edu
On May 8, 2009, at 8:04 AM, connellyps-at-nhlbi.nih.gov wrote:
} I do not recall the name/type of the tubing } that I used but it was milky white in color, not very flexible and } about 8mm } in diameter. Someone on the List will most likely tell us the name.
Dear Pat, Two of the more likely possibilities are polyethylene and teflon. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 14, 36 -- From wesaia-at-iastate.edu Fri May 8 16:17:53 2009 14, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 14, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48LHqDc017138 14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:53 -0500 14, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 14, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n48LHpof018415 14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:51 -0500 14, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp 14, 36 -- id 2486_b0b46180_3c15_11de_ad46_001372578af6; 14, 36 -- Fri, 08 May 2009 16:17:52 -0500 14, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 14, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n48LHotq014983 14, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 8 May 2009 16:17:50 -0500 14, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 36 -- Fri, 8 May 2009 16:17:50 -0500 14, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 36 -- Content-class: urn:content-classes:message 14, 36 -- MIME-Version: 1.0 14, 36 -- Content-Type: text/plain; 14, 36 -- charset="us-ascii" 14, 36 -- Subject: RE: [Microscopy] viaWWW: Nitrogen leak 14, 36 -- Date: Fri, 8 May 2009 16:18:50 -0500 14, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE7301-at-maire.eng.iastate.edu} 14, 36 -- In-Reply-To: {200905081746.n48HkUJH023088-at-ns.microscopy.com} 14, 36 -- X-MS-Has-Attach: 14, 36 -- X-MS-TNEF-Correlator: 14, 36 -- Thread-Topic: [Microscopy] viaWWW: Nitrogen leak 14, 36 -- Thread-Index: AcnQBOy16Wcj62O3QJObB5esGN629AAG5Mkg 14, 36 -- References: {200905081746.n48HkUJH023088-at-ns.microscopy.com} 14, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 14, 36 -- To: {Microscopy-at-microscopy.com} 14, 36 -- X-OriginalArrivalTime: 08 May 2009 21:17:50.0944 (UTC) FILETIME=[718F1E00:01C9D022] 14, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.8.210440 14, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0' 14, 36 -- Content-Transfer-Encoding: 8bit 14, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48LHqDc017138 ==============================End of - Headers==============================
In the past regular PE (not the cross-linked PEX) was commonly used for both water and air. If there was a situation that had elevated temperature or pressure, then nylon was the alternative. Polyurethane has been commonly used for water, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Friday, May 08, 2009 5:20 PM To: kenconverse-at-qualityimages.biz
I would suspect polyethylene. It sounds a lot like the PEX (polyethylene cross-linked) pipe used in home domestic water applications. Teflon would probably be too flexible. It would be too expensive for the thickness required to stand the pressure.
Warren S.
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Friday, May 08, 2009 12:47 PM To: wesaia-at-iastate.edu
On May 8, 2009, at 8:04 AM, connellyps-at-nhlbi.nih.gov wrote:
} I do not recall the name/type of the tubing } that I used but it was milky white in color, not very flexible and } about 8mm } in diameter. Someone on the List will most likely tell us the name.
Dear Pat, Two of the more likely possibilities are polyethylene and teflon. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
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==============================Original Headers============================== 25, 25 -- From kenconverse-at-qualityimages.biz Fri May 8 17:01:47 2009 25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48M1jRf009521 25, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 17:01:46 -0500 25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com 25, 25 -- with ESMTP 25, 25 -- id {20090508220143359.OQQU17201-at-cdptpa-omta01.mail.rr.com} ; 25, 25 -- Fri, 8 May 2009 22:01:43 +0000 25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 25 -- To: {wesaia-at-iastate.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 25, 25 -- Subject: RE: [Microscopy] RE: viaWWW: Nitrogen leak 25, 25 -- Date: Fri, 8 May 2009 18:01:36 -0400 25, 25 -- Message-ID: {920E986ABC46420688CF6FC4A0CDD472-at-Ken} 25, 25 -- MIME-Version: 1.0 25, 25 -- Content-Type: text/plain; 25, 25 -- charset="us-ascii" 25, 25 -- X-Priority: 3 (Normal) 25, 25 -- X-MSMail-Priority: Normal 25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 25, 25 -- In-Reply-To: {200905082119.n48LJquT021902-at-ns.microscopy.com} 25, 25 -- Importance: Normal 25, 25 -- Thread-Index: AcnQIr3i0yfXasasR3G907pI6f786wABVAnw 25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 25, 25 -- Content-Transfer-Encoding: 8bit 25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n48M1jRf009521 ==============================End of - Headers==============================
1. The Nanoprobe Network is hosting our third on-line Live Forum on ***Friday, May 22 from 11:30 AM to 1:00 PM, Eastern Daylight Time*.** The discussion topic will be “*Piezoresponse Force Microscopy*”. You are invited to post comments on the Nanoprobe Network Forum anytime prior to, during, or after the event (http://nanoprobenetwork.org/forum). During the Live Forum, AFM experts from around the globe will discuss issues, ideas, and questions regarding the applications, principles, recent advances and technical know-how of piezoresponse force microscopy of ferroelectrics, multiferroics, and biological systems. The questions you will be able to ask can range from:
* how can I implement PFM on a microsope * how do I distinguish the PFM image from cross-talk * how to acquire high-resolution spectroscopic data , and how to interpret it * what are the advantages and pitfalls of resonance enhanced PFM * can PFM be done in liquid * and many others.
Special expert guests for this chat will be Dr. Sergei Kalinin from Oak Ridge National Laboratory, Dr. Andrei Kholkin from the University of Aveiro, Portugal, and Dr. Alexei Gruverman from the University of Nebraska. (http://nanoprobenetwork.org/bbpress/forum.php?id=26)
This text-based forum allows any Nanoprobe Network member to ask and answer questions, propose ideas, suggest literature, and explore any topic of interest related to PFM. To participate, you must be a member of the Nanoprobe Network. Registration is free. Simply go to http://www.nanoprobenetwork.org, and click on the "Register" button in the upper right.
We hope you find the Nanoprobe Network resource beneficial to your work and we look forward to seeing you on-line!
Cordially, Hong-Mei Li Nanoprobe Network Manager http://nanoprobenetwork.org
P.S. *_Recent Posts_*
May 7, 2009 Live Forum Series: Piezoresponse Force Microscopy http://nanoprobenetwork.org/bbpress/forum.php?id=26
May 6, 2009 Veeco AFM Basic Training Course with the Innova AFM http://nanoprobenetwork.org/veeco-afm-basic-training-course-with-the-innova-afm
Veeco AFM Webinar Series: Advances AFM Applications Using the New Dimension Icon http://nanoprobenetwork.org/veeco-afm-webinar-series-advances-afm-applications-using-the-new-dimension-icon
-- Hong-Mei Li Nanoprobe Network Administrator Nano/Bio Interface Center (NBIC) University of Pennsylvania
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] Polishing Plexiglass
Question: Greetings!
I need to polish scratched plexiglass. Does anyone have any experience with plexiglass prep? Any information on what abrasives to use or which suspensions to avoid?
Thanks to those who gave me some good feedback about the 570 and 4700. One suggestion was to call Hitachi sales and ask about the legacy of systems and if anyone was upgrading. No one seems to be upgrading at this time. But I did come up with two suggested systems that seem to fit my budget. I would appreciate feedback on these, pro and con. I would like to find a system in my budget range (~$20K) that has DP or turbo, type II stage, specimen interlock and has all manuals--operation, maintenance, schematics, and bakeout items. Some have suggested using dry roughing pumps instead of rotary vane pumps due to backstreaming and fouling the EDS window.
S-2700 is the last LaB6 Hitachi produced.
S-4500 is cir 1991 and was the first to have in-lens/TLD dual detectors.
S-4200 has one SE.
The S-4700 is way over budget and the S-570 is unsupportable due to lack of spare parts--especially V1.
All input is appreciated. I'm not in a hurry to buy a system, but I would like to know what to be on the lookout for. I can add Quartz PCI digital imaging capture or use the EDS scan generator. But the SEM has to have an EDS port, conical lens, and be user friendly for self-service to a maximum extent.
gary g.
==============================Original Headers============================== 9, 17 -- From gary-at-gaugler.com Fri May 8 19:19:12 2009 9, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n490JAwn024561 9, 17 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 19:19:11 -0500 9, 17 -- Message-Id: {200905090019.n490JAwn024561-at-ns.microscopy.com} 9, 17 -- Received: (qmail 779 invoked from network); 8 May 2009 17:13:20 -0700 9, 17 -- Received: by simscan 1.1.0 ppid: 776, pid: 777, t: 0.0806s 9, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 17 -- by smtp2 with SMTP; 8 May 2009 17:13:20 -0700 9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 17 -- Date: Fri, 08 May 2009 17:18:56 -0700 9, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 9, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 17 -- Subject: Which Hitachi SEM 9, 17 -- Mime-Version: 1.0 9, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Marissa, There is an application note on our website for polishing polystyrene disks, entitled, " Optical Polishing of Polystyrene Discs to Specific Dimensions". The link is http://www.southbaytech.com/appnotes/26%20Optical%20Polishing%20of%20Polysty rene%20Discs%20to%20Specific%20Dimensions.PDF
It may be a little overkill for what you need, but it should be similar to what you need to do or at least to get you started.
Disclaimer: SBT manufactures and sells lapping and polishing equipment and consumables for materials processing.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com swalck-at-southbaytech.com
-----Original Message----- X-from: mlibbee-at-gmail.com [mailto:mlibbee-at-gmail.com] Sent: Friday, May 08, 2009 4:28 PM To: swalck-at-southbaytech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] Polishing Plexiglass
Question: Greetings!
I need to polish scratched plexiglass. Does anyone have any experience with plexiglass prep? Any information on what abrasives to use or which suspensions to avoid?
Dear List, I am trying to email Nigel, but the address I got from the MSA directory bounced; could anyone send me a working eaddress for him? TIA. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Fri May 8 20:11:20 2009 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n491BKgO021563 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 20:11:20 -0500 2, 20 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by earth-doxen-postvirus (Postfix) with ESMTP id 481C366E437D 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:11:20 -0700 (PDT) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 2, 20 -- by earth-doxen-ssl (Postfix) with ESMTP id 6FC5666E42BD 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:11:19 -0700 (PDT) 2, 20 -- Message-Id: {3DA1D736-75A7-4A45-8789-667D304FEF52-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v930.3) 2, 20 -- Subject: Email address for Nigel Unwin 2, 20 -- Date: Fri, 8 May 2009 18:11:18 -0700 2, 20 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
From misty-at-cybersource.com Sun May 10 14:08:23 2009 Return-Path: {misty-at-cybersource.com} Received: from google.com (cpc3-basf6-0-0-cust955.nott.cable.ntl.com [86.1.191.188]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4AJ8KYH006253 for {microscopylistserverarchive-at-microscopy.com} ; Sun, 10 May 2009 14:08:22 -0500 Received: from [114.198.81.154] (HELO google.com) by cleanranked.org; Sun, 10 May 2009 20:10:44 +0100 Message-ID: {000000040D0724C963243905} Reply-To: Rexanne Baum {audrea.sivils14212-at-gmail.com}
Hi, Marissa
Here in New Zealand there is a brass polish on the market called "Brasso" which can remove fine scratching and produce a fine polish. I imagine there are similar products in other countries.
If the scratches are deeper than Brasso can remove, you can use really fine emery paper (800 or even 1200) then polish with Brasso.
But try just the Brasso first, as you should use the emery paper only if you need to. If you do have to use the emery paper, you may be horrified at what it does, however, with Brasso and lots of rubbing (with a soft cloth) you will get there. You may even conjure up a genie, although I never have.
cheers Ritchie Sims Geology University of Auckland
} Email: mlibbee-at-gmail.com } Name: Marissa Libbee } } Title-Subject: [Filtered] Polishing Plexiglass } } Question: Greetings! } } I need to polish scratched plexiglass. Does anyone have any } experience with plexiglass prep? Any information on what abrasives } to use or which suspensions to avoid? } } Thanks! }
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==============================Original Headers============================== 11, 42 -- From r.sims-at-auckland.ac.nz Sun May 10 18:20:53 2009 11, 42 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4ANKlH5016089 11, 42 -- for {microscopy-at-microscopy.com} ; Sun, 10 May 2009 18:20:52 -0500 11, 42 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 42 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 80655668141; 11, 42 -- Mon, 11 May 2009 11:20:46 +1200 (NZST) 11, 42 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 11, 42 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 11, 42 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 11, 42 -- with ESMTP id FN-h5D4LgJjL; Mon, 11 May 2009 11:20:46 +1200 (NZST) 11, 42 -- Received: from uxchorprd02.its.auckland.ac.nz (uxchorprd02.its.auckland.ac.nz [130.216.10.23]) 11, 42 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 11, 42 -- (No client certificate requested) 11, 42 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 5EBCA668140; 11, 42 -- Mon, 11 May 2009 11:20:44 +1200 (NZST) 11, 42 -- Received: from uxchorprd02.its.auckland.ac.nz (localhost.localdomain [127.0.0.1]) 11, 42 -- by uxchorprd02.its.auckland.ac.nz (8.13.8/8.13.8) with ESMTP id n4ANKhYD013697; 11, 42 -- Mon, 11 May 2009 11:20:43 +1200 11, 42 -- Received: (from apache-at-localhost) 11, 42 -- by uxchorprd02.its.auckland.ac.nz (8.13.8/8.13.8/Submit) id n4ANKfIv013694; 11, 42 -- Mon, 11 May 2009 11:20:41 +1200 11, 42 -- X-Authentication-Warning: uxchorprd02.its.auckland.ac.nz: apache set sender to r.sims-at-auckland.ac.nz using -f 11, 42 -- Received: from 124-197-8-235.callplus.net.nz (124-197-8-235.callplus.net.nz 11, 42 -- [124.197.8.235]) by webmail.auckland.ac.nz (Horde Framework) with HTTP; 11, 42 -- Mon, 11 May 2009 11:20:40 +1200 11, 42 -- Message-ID: {20090511112040.17906jqc4jdjus8w-at-webmail.auckland.ac.nz} 11, 42 -- Date: Mon, 11 May 2009 11:20:40 +1200 11, 42 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz} 11, 42 -- To: microscopy-at-microscopy.com 11, 42 -- Cc: mlibbee-at-gmail.com 11, 42 -- Subject: Re: [Microscopy] viaWWW: Polishing Plexiglass 11, 42 -- References: {200905082319.n48NJMSW008874-at-ns.microscopy.com} 11, 42 -- In-Reply-To: {200905082319.n48NJMSW008874-at-ns.microscopy.com} 11, 42 -- MIME-Version: 1.0 11, 42 -- Content-Type: text/plain; 11, 42 -- charset=ISO-8859-1; 11, 42 -- DelSp="Yes"; 11, 42 -- format="flowed" 11, 42 -- Content-Disposition: inline 11, 42 -- Content-Transfer-Encoding: 7bit 11, 42 -- User-Agent: Internet Messaging Program (IMP) 4.3.3 ==============================End of - Headers==============================
Depending on how deep the scratches are, I've had lots of success using a standard auto headlight polishing kit (Meguiar's Headlight and Clear Plastic Restoration Kit) but I do all the sanding and polishing by hand - it's too easy to ruin something using a drill. Note: I've done this to restore appearance and visual clarity only, if you need to capture images through the plexiglass, you'll need precision at a level far greater than you'll ever achieve by hand.
I have no connection to this company other than as a satisfied customer
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal. ================================================
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] Polishing Plexiglass
Question: Greetings!
I need to polish scratched plexiglass. Does anyone have any experience with plexiglass prep? Any information on what abrasives to use or which suspensions to avoid?
Thanks!
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==============================Original Headers============================== 14, 34 -- From Robert.Zonis-at-Sanford.com Mon May 11 09:54:58 2009 14, 34 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243]) 14, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4BEsvnO014442 14, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 11 May 2009 09:54:57 -0500 14, 34 -- X-VirusChecked: Checked 14, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 14, 34 -- X-Msg-Ref: server-4.tower-192.messagelabs.com!1242053696!31680748!2 14, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 14, 34 -- X-Originating-IP: [198.176.16.25] 14, 34 -- Received: (qmail 10504 invoked from network); 11 May 2009 14:54:57 -0000 14, 34 -- Received: from naehub2.newellco.com (HELO naehub2.newellco.com) (198.176.16.25) 14, 34 -- by server-4.tower-192.messagelabs.com with SMTP; 11 May 2009 14:54:57 -0000 14, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub2.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 14, 34 -- Mon, 11 May 2009 09:54:57 -0500 14, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 34 -- Content-class: urn:content-classes:message 14, 34 -- MIME-Version: 1.0 14, 34 -- Content-Type: text/plain; 14, 34 -- charset="us-ascii" 14, 34 -- Subject: RE: [Microscopy] viaWWW: Polishing Plexiglass 14, 34 -- Date: Mon, 11 May 2009 09:52:09 -0500 14, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9402AD79D1-at-naoaksasebe02.nr.ad.newellco.com} 14, 34 -- In-Reply-To: {200905082324.n48NOgGB016573-at-ns.microscopy.com} 14, 34 -- X-MS-Has-Attach: 14, 34 -- X-MS-TNEF-Correlator: 14, 34 -- Thread-Topic: [Microscopy] viaWWW: Polishing Plexiglass 14, 34 -- thread-index: AcnQNCx+6NHas5cASxmV4n0uQq27TgCEp2xA 14, 34 -- References: {200905082324.n48NOgGB016573-at-ns.microscopy.com} 14, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 14, 34 -- To: {mlibbee-at-gmail.com} 14, 34 -- Cc: {Microscopy-at-microscopy.com} 14, 34 -- X-OriginalArrivalTime: 11 May 2009 14:54:57.0064 (UTC) FILETIME=[734C7A80:01C9D248] 14, 34 -- Content-Transfer-Encoding: 8bit 14, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4BEsvnO014442 ==============================End of - Headers==============================
Dear Users, I have a user who wants some morphometric data on his mitochondria. He wants to prove if they are like tubules or round (ball-like) and he needs a good reference source (textbook or article). Basically wants 3d info without having to do reconstructions. Any ideas?
Thanks, M Delannoy
==============================Original Headers============================== 2, 27 -- From delannoy-at-jhmi.edu Mon May 11 12:39:09 2009 2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4BHd9Wl004741 2, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 12:39:09 -0500 2, 27 -- X-IronPort-AV: E=Sophos;i="4.40,329,1238990400"; 2, 27 -- d="scan'208";a="211798483" 2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 11 May 2009 13:39:09 -0400 2, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu 2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 2, 27 -- with ESMTP id {0KJH00BM9QDAZF50-at-jesmail.johnshopkins.edu} for 2, 27 -- microscopy-at-microscopy.com; Mon, 11 May 2009 13:39:10 -0400 (EDT) 2, 27 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.59]) 2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 11 May 2009 13:39:10 -0400 2, 27 -- Date: Mon, 11 May 2009 13:39:10 -0400 2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 27 -- Subject: morphometry 2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 27 -- Message-id: {f524aec09dac.4a082a7e-at-johnshopkins.edu} 2, 27 -- MIME-version: 1.0 2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 2, 27 -- Content-type: text/plain; charset=us-ascii 2, 27 -- Content-language: en 2, 27 -- Content-transfer-encoding: 7BIT 2, 27 -- Content-disposition: inline 2, 27 -- X-Accept-Language: en 2, 27 -- Priority: normal ==============================End of - Headers==============================
mlibbee-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa Libbee } } Title-Subject: [Filtered] Polishing Plexiglass } } Question: Greetings! } } I need to polish scratched plexiglass. Does anyone have any } experience with plexiglass prep? Any information on what abrasives } to use or which suspensions to avoid? } } Thanks! } } Login Host: 209.3.42.11 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Fri May 8 18:18:07 2009 } 7, 11 -- Received: from [130.202.238.72] (msdvpn072.msd.anl.gov [130.202.238.72]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n48NI3Gh007468 } 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 8 May 2009 18:18:05 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240802c62a6e1c420e-at-[130.202.238.72]} } 7, 11 -- Date: Fri, 8 May 2009 18:18:08 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Polishing Plexiglass } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } }
-- Milen Shishkov Wellman Center for Photomedicine Massachusetts General Hospital Harvard Medical School
Address: MGH BAR 712 50 Blossom St. Boston, MA 02114 Phone: (617) 726 1589 Fax: (617) 726 4103
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==============================Original Headers============================== 9, 24 -- From SHISHKOV-at-HELIX.MGH.HARVARD.EDU Mon May 11 12:55:30 2009 9, 24 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4BHtT86019746 9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 12:55:29 -0500 9, 24 -- X-IronPort-AV: E=Sophos;i="4.40,329,1238990400"; 9, 24 -- d="scan'208";a="80897428" 9, 24 -- Received: from phsxcon1.partners.org ([132.183.130.40]) 9, 24 -- by phsmgmx11.partners.org with ESMTP; 11 May 2009 13:55:29 -0400 9, 24 -- Received: from [132.183.44.113] ([132.183.44.113]) by PHSXCON1.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 9, 24 -- Mon, 11 May 2009 13:55:29 -0400 9, 24 -- Message-ID: {4A08668D.5020405-at-helix.mgh.harvard.edu} 9, 24 -- Date: Mon, 11 May 2009 13:55:25 -0400 9, 24 -- From: Milen Shishkov {shishkov-at-HELIX.MGH.HARVARD.EDU} 9, 24 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 9, 24 -- MIME-Version: 1.0 9, 24 -- To: mlibbee-at-gmail.com 9, 24 -- CC: microscopy-at-microscopy.com 9, 24 -- Subject: Re: [Microscopy] viaWWW: Polishing Plexiglass 9, 24 -- References: {200905082319.n48NJkar009405-at-ns.microscopy.com} 9, 24 -- In-Reply-To: {200905082319.n48NJkar009405-at-ns.microscopy.com} 9, 24 -- X-OriginalArrivalTime: 11 May 2009 17:55:29.0486 (UTC) FILETIME=[ABECE6E0:01C9D261] 9, 24 -- Content-Type: text/plain; charset="iso-8859-1"; format="flowed" 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4BHtT86019746 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both david.mitchell-at-emu.usyd.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: david.mitchell-at-emu.usyd.edu.au Name: David Mitchell
I am commissioning a standard JEOL2200FS with an omega filter, and am looking at the EELS resolution. Routinely I can get 1.0eV resolution (-at- 200kV) and if I throttle back A1 on the gun, I can get 0.9eV. I am wondering if this is in the right ballpark, and if anyone is doing much better, how are they doing it?
Thanks and regards
Dave Mitchell
Dr David Mitchell Senior Microscopist, Transmission Electron Microscopy
Address: Electron Microscope Unit Australian Key Centre for Microscopy and Microanalysis Australian Microscopy & Microanalysis Research Facility (AMMRF) Madsen Building F09, Room 142A The University of Sydney NSW 2006, Australia www.emu.usyd.edu.au www.ammrf.org.au
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nama1213-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Does any one knows how to prepare plant samples for Atomic Force Microscope (AFM). Basically I need to (1) measure roughness of leaf surface and stomata, (2) prepare DNA for AFM and (3) capture images of pollen grain (do I need to grind pollen into fine powder) and (4) prpeare bacterial culture for AFM studies. I have tried some techniques but would like to get help on published techniques that anyone tried previously.
Many thanks and looking forward to hearing from you colleagues.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jacqueline.williams-at-stjude.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jacqueline.williams-at-stjude.org Name: jackie williams
Organization: St. Jude Children's Research Hospital
Title-Subject: [Filtered] Disposal of sodium cacodylate 0.1M
Question: There has been some discussions regarding the disposal of sodium cacodylate. For numerous years we have disposed of the buffer down the drain with copious amounts of water. Now there is questions about the arsenic content. What are the current disposal rules? Thanks in advance for any suggestions.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cox_j-at-gwmail.gwu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cox_j-at-gwmail.gwu.edu Name: Jonathan Cox
Organization: The George Washington University
Title-Subject: [Filtered] TEM
Question: We recently had a digital camera installed on our TEM and I'm currently calibration the various magnifications. Can anyone point towards or send me an image of how to use catalased crystals for calibrations of the higher magnifications? Thank you in advance.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Laabs-at-ameslab.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Laabs-at-ameslab.gov Name: Fran Laabs
Organization: Ames Lab, ISU
Title-Subject: [Filtered] Polishing Plexiglass
Question: This is in respose to Marissa's question regarding how to polish Plexiglas. There is a product called "Micromesh" sold by Wag-Aero (www.wagaero.com). It is used to remove scratches from aircraft windscreens and I can personally atest to the fact it works well. Fran Laabs
Attached is my personal summary for image calibration with catalase crystals.
(summary document not attached for the Listserver.. can send by request. It is simply my notes with a scan of the image in the Wrigley paper....) the text is below.
Catalase standard for magnification ================================================================== Useful in the 10,000X to 200,000X range.
Nicholas G. Wrigley. The Lattice Spacing of Crystalline Catalase as an Internal Standard of Length in Electron Microscopy. J. Ultrastructural Research 24, 454-464. 1968.
The Wrigley paper describes recrystallization followed by glutaraldehyde fixation to give thin platelet crystals that do not dissolve. The spacing used for calibration is "1/2 Co"; there are a couple of values given in the paper, 86 A or 87.5 A. This is the "obvious" line spacing perpendicular to the long crystal axis (see image). Note that the image is well filtered by reinforcement (old school method, pre computer) and raw images do not look like this. There is a 68.5 A spacing parallel to the long axis, Ao, that is less useful.
SPI sells catalase prepared by the Wrigley method. Others probably do also. My bottle is from SPI and 15yr old and still good.
Apply the suspension to a glow-discharge treated carbon filmed grid and allow to settle and adsorb until almost dry. Wick away the excess and add a drop of 4% sodium silicotungstate, allowing 10-30s to penetrate, and then wick excess and air dry the grid. 3% Ammonium molybdate and 2% Uranyl Acetate are also used; Wrigley used the sodium silicotungstate.
Wrigley specifically states to NOT use the spacing at the edges of the crystal since the spacing can be distorted. This image is from the Wrigley paper.......
Method for prep. The details are in the Wrigley paper. Make a suspension dilution in distilled water that is slightly turbid and apply to hydrophilic (glow-discharged) thin pure carbon films and negative stain with sodium silico-tungstate as given in the Wrigley paper. Not every crystal will be good and the staining will be better on some than others. Look at the crystal edges where the crystal becomes more transparent. Look for areas that show the lattice well and photograph at each mag needed. The images can be measured and the calibration factors placed into the camera software (consult your documentation - every camera is different). Bottom line is that you will measure the spacings in the digital image (measure multiple repeats for best accuracy) and that # of pixels is equal to (spacing x #repeats) and that calibration is entered somewhere (your camera software, the ImageJ plugin, etc.) I have a writeup on how to have multiple calibrations (from different cameras for instance) in an ImageJ plugin and choose on the fly. http://www.bio.umass.edu/microscopy/resource.htm and see the "Image Calibrations with ImageJ" section.
Hope this helps,
Dale Callaham Umass Amherst
cox_j-at-gwmail.gwu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both cox_j-at-gwmail.gwu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: cox_j-at-gwmail.gwu.edu } Name: Jonathan Cox } } Organization: The George Washington University } } Title-Subject: [Filtered] TEM } } Question: We recently had a digital camera installed on our TEM and } I'm currently calibration the various magnifications. Can anyone } point towards or send me an image of how to use catalased crystals } for calibrations of the higher magnifications? Thank you in advance. } } Login Host: 128.164.239.33 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:58:20 2009 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C0wJ8l029783 } 6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 19:58:20 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240806c62e7a0bb4cc-at-[206.69.208.22]} } 6, 11 -- Date: Mon, 11 May 2009 19:58:18 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: cox_j-at-gwmail.gwu.edu (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: TEM Mag calibration } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 20 -- From dac-at-research.umass.edu Mon May 11 21:52:41 2009 17, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C2qe6b002157 17, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 May 2009 21:52:40 -0500 17, 20 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged)) 17, 20 -- (authenticated bits=0) 17, 20 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n4C2qdWM002589 17, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 17, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 May 2009 22:52:40 -0400 17, 20 -- Message-ID: {4A08E47C.5070702-at-research.umass.edu} 17, 20 -- Date: Mon, 11 May 2009 22:52:44 -0400 17, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 17, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16 17, 20 -- MIME-Version: 1.0 17, 20 -- To: Microscopy-at-microscopy.com 17, 20 -- Subject: Re: [Microscopy] viaWWW: TEM Mag calibration 17, 20 -- References: {200905120107.n4C17icA030084-at-ns.microscopy.com} 17, 20 -- In-Reply-To: {200905120107.n4C17icA030084-at-ns.microscopy.com} 17, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We got .7 to .6 on a 12 year old 2010F with a Tridiem at the highest dispersion. I would think you should just as well with a 2200. You did not indicate what dispersion you were using. John Mardinly
Sent from my iPhone
On May 11, 2009, at 6:16 PM, david.mitchell-at-emu.usyd.edu.au wrote:
} } } } --- } --- } ---------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --- } --- } ---------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --- } --- } --------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both david.mitchell-at-emu.usyd.edu.au as well as the } MIcroscopy Listserver } --- } --- } --------------------------------------------------------------------- } } Email: david.mitchell-at-emu.usyd.edu.au } Name: David Mitchell } } Organization: EMU, University of Sydney } } Title-Subject: [Filtered] TEM: JEOL 2200FS EELS resolution } } Question: Dear All } } I am commissioning a standard JEOL2200FS with an omega filter, and am } looking at the EELS resolution. Routinely I can get 1.0eV resolution } (-at- 200kV) and if I throttle back A1 on the gun, I can get 0.9eV. I am } wondering if this is in the right ballpark, and if anyone is doing } much better, how are they doing it? } } Thanks and regards } } Dave Mitchell } } } Dr David Mitchell } Senior Microscopist, Transmission Electron Microscopy } } Contact: } PH +61 2 9036 7633 } FAX +61 2 9351 7682 } David.mitchell-at-emu.usyd.edu.au } } Address: } Electron Microscope Unit } Australian Key Centre for Microscopy and Microanalysis } Australian Microscopy & Microanalysis Research Facility (AMMRF) } Madsen Building F09, Room 142A } The University of Sydney } NSW 2006, Australia } www.emu.usyd.edu.au } www.ammrf.org.au } } } Login Host: 129.78.64.100 } --- } --- } --------------------------------------------------------------------- } } ==============================Original } Headers============================== } 14, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:56:22 2009 } 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22 } ]) } 14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4C0uKo2025265 } 14, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 } 19:56:21 -0500 } 14, 11 -- Mime-Version: 1.0 } 14, 11 -- Message-Id: {p06240803c62e79a49c97-at-[206.69.208.22]} } 14, 11 -- Date: Mon, 11 May 2009 19:56:20 -0500 } 14, 11 -- To: microscopy-at-microscopy.com } 14, 11 -- From: david.mitchell-at-emu.usyd.edu.au (by way of } MicroscopyListserver) } 14, 11 -- Subject: viaWWW: TEM: JEOL 2200FS EELS resolution } 14, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
TAP Plastics, a US West Coast plastics company, specializes in Plexiglas (AKA Perspex/Acrylite /acrylic, etc. for our non-US readers) (as well as cast acrylic resin, fiberglass, and carbon fiber) materials for the home hobbyist and other interested parties (such as scientists!). TAP's website has good technical information.
http://www.tapplastics.com/
For polishing and finishing, you may want to look at the following: (.pdf) http://www.tapplastics.com/uploads/pdf/Tech%20Data-Edge.pdf http://www.tapplastics.com/uploads/pdf/a_working_acrylite.pdf
TAP has a video that you may find helpful: http://www.tapplastics.com/info/video.php#video26
also posted on YouTube: http://www.youtube.com/watch?v=4yp-kumgpoM
usual disclaimer: satisfied customer.
-------- Original Message --------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] Polishing Plexiglass
Question: Greetings!
I need to polish scratched plexiglass. Does anyone have any experience with plexiglass prep? Any information on what abrasives to use or which suspensions to avoid?
Marc Takeno, Ph.D. | Research Scientist | University of Washington Department of Bioengineering | N330B Foege | Box 355061 (206) 543-4290 | takenomm-at-u.washington.edu
==============================Original Headers============================== 17, 27 -- From takenomm-at-u.washington.edu Tue May 12 00:02:56 2009 17, 27 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4C52uXD006756 17, 27 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 00:02:56 -0500 17, 27 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.7] (may be forged)) 17, 27 -- by mxout4.cac.washington.edu (8.14.3+UW09.03/8.14.3+UW09.03) with ESMTP id n4C52tBW001224 17, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK); 17, 27 -- Mon, 11 May 2009 22:02:55 -0700 17, 27 -- X-Auth-Received: from [192.168.1.112] (75-172-97-226.tukw.qwest.net [75.172.97.226]) 17, 27 -- (authenticated authid=takenomm) 17, 27 -- by smtp.washington.edu (8.14.3+UW09.03/8.14.3+UW09.01) with ESMTP id n4C52rQ5014019 17, 27 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 17, 27 -- Mon, 11 May 2009 22:02:55 -0700 17, 27 -- Message-ID: {4A0902FE.4090104-at-u.washington.edu} 17, 27 -- Date: Mon, 11 May 2009 22:02:54 -0700 17, 27 -- From: Marc Takeno {takenomm-at-u.washington.edu} 17, 27 -- Organization: University of Washington 17, 27 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 17, 27 -- MIME-Version: 1.0 17, 27 -- To: microscopy-at-microscopy.com 17, 27 -- CC: mlibbee-at-gmail.com 17, 27 -- Subject: [Microscopy] viaWWW: Polishing Plexiglass 17, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 27 -- Content-Transfer-Encoding: 7bit 17, 27 -- X-PMX-Version: 5.5.4.371499, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2009.5.12.45227 17, 27 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report=' 17, 27 -- BODY_SIZE_1400_1499 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MOZILLA_MSGID 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0, __USER_AGENT 0' ==============================End of - Headers==============================
I need to perform a SEM analysis of virus particles, attached to fibers, after immunogold labeling of the particles. However, I do not have any experience with immunogold and SEM. Please can you give me some advice, protocols, tips and trics, …
Kind regards, Wim.
------------------------------------------------------- Wim Van den Broeck, DVM, MSc, PhD Department of Morphology Laboratory of Cytology and Histology Faculty of Veterinary Medicine, Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM tel.: +32 (0)9 264 77 16 fax: +32 (0)9 264 77 90 Email: wim.vandenbroeck-at-UGent.be ------------------------------------------------------- ï Please consider the environment before printing this e-mail. Please add this line to your email to tell others.
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I recently accepted a position in an E.M. lab with a Morgagni TEM. Previous users have damaged several of the specimen tips for the TEM, and I am writing the List to see if anyone has a connection with someone that repairs the tips. The tips are not the same size as those from 200 or 300 series TEM's from Philips. Thanks in advance for the input!
Ed Haller University of South Florida Integrative Biology Tampa, FL 33620 813-974-2676
==============================Original Headers============================== 7, 35 -- From ehaller-at-health.usf.edu Tue May 12 07:28:27 2009 7, 35 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CCSQPU029446 7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 07:28:27 -0500 7, 35 -- X-ASG-Debug-ID: 1242131303-57ec013b0000-4CH8be 7, 35 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-bin/mark.cgi 7, 35 -- Received: from mailhub1.hscnet.hsc.usf.edu (localhost [127.0.0.1]) 7, 35 -- by hscantispam.health.usf.edu (Spam Firewall) with ESMTP id 20DD44094D87 7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:28:23 -0400 (EDT) 7, 35 -- Received: from mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) by hscantispam.health.usf.edu with ESMTP id QQHQlhTfRBJoOcDU for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:28:23 -0400 (EDT) 7, 35 -- Received: from MAILSERVER2.hscnet.hsc.usf.edu ([10.119.4.17]) by 7, 35 -- mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) with mapi; Tue, 12 May 2009 7, 35 -- 08:28:24 -0400 7, 35 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 7, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 35 -- Date: Tue, 12 May 2009 08:26:56 -0400 7, 35 -- X-ASG-Orig-Subj: RE: Information About Repairing Specimen Tips--FEI Morgagni TEM 7, 35 -- Subject: RE: Information About Repairing Specimen Tips--FEI Morgagni TEM 7, 35 -- Thread-Topic: Information About Repairing Specimen Tips--FEI Morgagni TEM 7, 35 -- Thread-Index: AcnOeLxmIBvIeUBJTgmLdQ+hBXyNLAEhDPCF 7, 35 -- Message-ID: {7D1470DAD69A6744AE69D98054321F340112A26E7746-at-MAILSERVER2.hscnet.hsc.usf.edu} 7, 35 -- References: {200905061830.n46IUQjX007432-at-ns.microscopy.com} 7, 35 -- In-Reply-To: {200905061830.n46IUQjX007432-at-ns.microscopy.com} 7, 35 -- Accept-Language: en-US 7, 35 -- Content-Language: en-US 7, 35 -- X-MS-Has-Attach: 7, 35 -- X-MS-TNEF-Correlator: 7, 35 -- acceptlanguage: en-US 7, 35 -- Content-Type: text/plain; charset="us-ascii" 7, 35 -- MIME-Version: 1.0 7, 35 -- X-Barracuda-Connect: UNKNOWN[10.119.4.12] 7, 35 -- X-Barracuda-Start-Time: 1242131304 7, 35 -- X-Barracuda-Virus-Scanned: by USF Health AntiSpam System at health.usf.edu 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CCSQPU029446 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.g.phillips-at-sussex.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: r.g.phillips-at-sussex.ac.uk Name: Roger Phillips
Organization: University of Sussex
Title-Subject: [Filtered] MRC studentships for MSc in Imaging in Biomedical Research
Question: Dear Colleagues, The University of Sussex MSc programme in 'Imaging in Biomedical Research' has seven MRC-funded studentships on offer to UK and EU students for study beginning this Autumn. The programme is designed for good graduates in biological sciences who wish to acquire a strong grounding in imaging including physical and mathematical approaches before starting a research career. We have taken the liberty of addressing this specifically to the Microscopy Listserver as we hope that the programme will produce graduates who many of you would find well-suited to starting a PhD in your laboratory. We would be very grateful if you could forward this information to your graduating class of students.
'Imaging in Biomedical Research' is a new interdisciplinary programme in the Department of Biochemistry taught in collaboration with the University of Sussex Departments of Physics and Astronomy and Informatics and with the Brighton and Sussex Medical School Clinical Imaging Science Centre and the Sussex Centre for Advanced Microscopy. The programme provides a strong background for a career in research in the academic, clinical and industrial sectors. The student is taught theory, applications and practice in a broad range of imaging techniques including Electron Microscopy, Fluorescence Microscopy, Magnetic Resonance Imaging and Computed Tomography. Training in mathematical computation and in digital imaging provides the basis for understanding advanced processing and analyses and will include hands-on experience with practical computing languages such as Matlab and ImageJ. Students will benefit from access to both high content and high throughput microscopy facilities which are essential tools of pharmacological research and development. Options in the Autumn and Spring terms allow each student to specialise in different areas of biomedical imaging such as Molecular, Neuro or Developmental Biology. In the Summer term each student will plan and execute a research project under joint supervision of a member of faculty in one of the collaborating Departments and staff of one of the imaging centres.
All UK (home student) applications received by 17 July 2009 will be considered for funding and MRC Studentships will be awarded to the top applicants. EU students are eligible for tuition but not for stipend on MRC studentships. See our prospectus http://www.sussex.ac.uk/Units/publications/pgrad2009/areasofstudy/Biochemistry/22178 and the Centre for Advanced Microscopy website http://www.sussex.ac.uk/lifesci/1-4-30.html for further information.
I'm looking for suggestions for a possible cause for a recently occurring SEM image problem.
In the last 24 hours our JEOL JSM-5800 has developed a slow image flutter. It's very slow and almost relaxing. It's not the jaggies you see from 60 cycle scan interference, nor is it the jumping you sometimes see with charging. It seems more noticeable at 2kv as compared to 25kv, but only just.
I'm examining polished copper in a conductive support media. I have in the past done this and never experienced a problem. The problem doesn't seem to be time dependent, I see the same problem at 4:15pm (most people have turned off their equipment) and at 8:00am (everything is running full tilt!)%
We are experiencing a new noise in out of our chiller, possible a minor surge problem. I believe the chiller only cools the diffusion pump and not any of the lens control circuits. I could be wrong about this.
Any suggestion would be welcome!
stay safe........
Frank
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==============================Original Headers============================== 13, 21 -- From frank_karl-at-lincolnelectric.com Tue May 12 07:59:09 2009 13, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CCx8OV013481 13, 21 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 07:59:08 -0500 13, 21 -- Subject: SEM image problem 13, 21 -- To: Microscopy-at-microscopy.com 13, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 13, 21 -- Message-ID: {OF8F1A06ED.1554F19D-ON852575B4.00460916-852575B4.00474D71-at-lincolnelectric.com} 13, 21 -- Date: Tue, 12 May 2009 08:58:48 -0400 13, 21 -- From: Frank_Karl-at-lincolnelectric.com 13, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 13, 21 -- 07, 2008) at 05/12/2009 08:58:49 AM, 13, 21 -- CD-MIME complete at 05/12/2009 08:58:49 AM, 13, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 13, 21 -- 07, 2008) at 05/12/2009 08:58:49 AM, 13, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 13, 21 -- 07, 2008) at 05/12/2009 08:58:49 AM, 13, 21 -- Serialize complete at 05/12/2009 08:58:49 AM 13, 21 -- MIME-Version: 1.0 13, 21 -- Content-Type: text/plain; 13, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
Tom Schmelzer has been doing a great job on our sample rods.
TGS Technologies, LLC. 702 Little Creek Lane Cranberry Township PA 16066 USA Ph: 724.453.3865 Fax: 724.453.2968 E-mail: tom-at-tgstechnologies.net Website: http://www.tgstechnologies.net
Just a satisfied customer.
Cheers, Henk
At 08:31 AM 05/12/09, ehaller-at-health.usf.edu wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 11, 27 -- From colijn.1-at-osu.edu Tue May 12 08:04:56 2009 11, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CD4tok026671 11, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:04:56 -0500 11, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 11, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 11, 27 -- id {01N8UYABKCQO8ZWE58-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 11, 27 -- Tue, 12 May 2009 09:04:49 -0400 (EDT) 11, 27 -- Received: from HOC1.ecr6.ohio-state.edu 11, 27 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 11, 27 -- (PMDF V6.3-x18 #31556) 11, 27 -- with ESMTPA id {01N8UYA8H57K8Y2URF-at-ecr6.ohio-state.edu} ; Tue, 11, 27 -- 12 May 2009 09:04:43 -0400 (EDT) 11, 27 -- Date: Tue, 12 May 2009 09:06:02 -0400 11, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 27 -- Subject: Re: [Microscopy] RE: Information About Repairing Specimen Tips--FEI 11, 27 -- Morgagni TEM 11, 27 -- In-reply-to: {200905121231.n4CCVMbE031684-at-ns.microscopy.com} 11, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 27 -- To: ehaller-at-health.usf.edu 11, 27 -- Cc: Microscopy-at-microscopy.com 11, 27 -- Message-id: {01N8UYA8M45U8Y2URF-at-ecr6.ohio-state.edu} 11, 27 -- MIME-version: 1.0 11, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 27 -- References: {200905121231.n4CCVMbE031684-at-ns.microscopy.com} ==============================End of - Headers==============================
Jackie- Our Environmental Health Office insists that we collect all buffer wastes for pick up and disposal by them. Everything must be identified by content so that they can use the correct method. Lee
} --------------------------------------------------------------------------- } } Email: jacqueline.williams-at-stjude.org } Name: jackie williams } } Organization: St. Jude Children's Research Hospital } } Title-Subject: [Filtered] Disposal of sodium cacodylate 0.1M } } Question: There has been some discussions regarding the disposal of } sodium cacodylate. For numerous years we have disposed of the buffer } down the drain with copious amounts of water. Now there is questions } about the arsenic content. What are the current disposal rules? } Thanks in advance for any suggestions. } } Login Host: 192.55.208.10 } ---------------------------------------------------------------------------
-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
In spite of being a growth industry at our University, our Environmental Health and Safety Office has had to be prodded on this particular issue. They are still not sure how they should deal with UA solutions. We do as Leona - bottle and record relative concentrations of buffers with fixative, all but PBS without fixative (we do dump straight PBS and Hanks), waste stain solutions, etc. This is then taken down for disposal. The amounts are not great, and who knows what actually happens with these things when they get there. After all, I still remember the news story about the recycling program in the Toronto area which was recycling by sending the material to a land fill near Buffalo, or was it the other way around.
We are working on the assumption that someday EHSO will figure it out. In the meantime we are doing our best to be safe and environmentally responsible.
paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 7, 20 -- From paul_hazelton-at-umanitoba.ca Tue May 12 08:49:56 2009 7, 20 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CDntmG005592 7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:49:55 -0500 7, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 7, 20 -- (authenticated bits=0) 7, 20 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n4CDnrKM009824; 7, 20 -- Tue, 12 May 2009 08:49:53 -0500 (CDT) 7, 20 -- Message-ID: {4A097E78.6090602-at-umanitoba.ca} 7, 20 -- Date: Tue, 12 May 2009 08:49:44 -0500 7, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 20 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 7, 20 -- MIME-Version: 1.0 7, 20 -- To: jacqueline.williams-at-stjude.org 7, 20 -- Subject: Re: [Microscopy] viaWWW: Disposal of sodium cacodylate 0.1M 7, 20 -- References: {200905120100.n4C1012A003633-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200905120100.n4C1012A003633-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
perhaps you've got meanwhile some personal Re's from Listers. Since I have not seen any responses on the list I would like to recommend an OSHA-Document, assuming your location are the USA: http://www.osha.gov/SLTC/healthguidelines/arsenic/recognition.html
Find: Hazardous waste management requirements: "...Cacodylic acid is listed as a hazardous waste under RCRA and has been assigned EPA Hazardous Waste No. U136. This substance has been banned from land disposal."
Unfortunately I do not know about regulation of disposal of hydrous solutions in your particualr area and unfortunately I could not find any specific hint in the document above....
A similar problem I see within regulations for this Lab-waste in European regulatory rules.
So I collect all cacodylate buffer waste solutions into a bottle (needless to say that I keep the volume(s) as little as possible), and dispose of as "heavy metal containing" chemical waste solutions.
Perhaps this is - considering the minimal amount of As in the buffer - exaggerated .... but nevertheless in the limits of regulations for heavy metals assigned Toxic, hazardous and environmental pollutants.
Best regards,
Wolfgang MUSS SALZBURG/AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: jacqueline.williams-at-stjude.org } [mailto:jacqueline.williams-at-stjude.org] } Gesendet: Dienstag, 12. Mai 2009 03:04 } An: Muß Wolfgang } Betreff: [Microscopy] Disposal of sodium cacodylate 0.1M } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } -------------------------------------------------------------- } ------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both jacqueline.williams-at-stjude.org as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } ------------- } Email: jacqueline.williams-at-stjude.org } Name: jackie williams } Organization: St. Jude Children's Research Hospital } Title-Subject: } Disposal of sodium cacodylate 0.1M } Question: } } } There has been some discussions regarding the disposal of } sodium cacodylate. } For numerous years we have disposed of the buffer down the } drain with copious amounts of water. Now there is questions } about the arsenic content. What are the current disposal rules? } Thanks in advance for any suggestions. } } } } Login Host: 192.55.208.10 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Mon May 11 19:57:40 2009 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n4C0vdfY027377 } 6, 11 -- for {microscopy-at-microscopy.com} ; Mon, 11 May } 2009 19:57:40 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240805c62e79f1aeac-at-[206.69.208.22]} } 6, 11 -- Date: Mon, 11 May 2009 19:57:38 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Disposal of sodium cacodylate 0.1M } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 16, 36 -- From W.Muss-at-salk.at Tue May 12 08:55:24 2009 16, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CDtNPF012854 16, 36 -- for {microscopy-at-microscopy.com} ; Tue, 12 May 2009 08:55:23 -0500 16, 36 -- Received: from localhost (localhost [127.0.0.1]) 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id BB559C385B; 16, 36 -- Tue, 12 May 2009 15:55:20 +0200 (CEST) 16, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 16, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 16, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 16, 36 -- with ESMTP id qYAiuNg982Y1; Tue, 12 May 2009 15:55:20 +0200 (CEST) 16, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 4C0B6C3858; 16, 36 -- Tue, 12 May 2009 15:55:20 +0200 (CEST) 16, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 16, 36 -- Tue, 12 May 2009 15:55:20 +0200 16, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 36 -- Content-class: urn:content-classes:message 16, 36 -- MIME-Version: 1.0 16, 36 -- Content-Type: text/plain; 16, 36 -- charset="iso-8859-1" 16, 36 -- Subject: [Microscopy] Re: Disposal of sodium cacodylate 0.1M 16, 36 -- Date: Tue, 12 May 2009 15:55:20 +0200 16, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0A1E-at-N1RZ116.lksdom21.lks.local} 16, 36 -- In-Reply-To: {200905120103.n4C13VxW015674-at-ns.microscopy.com} 16, 36 -- X-MS-Has-Attach: 16, 36 -- X-MS-TNEF-Correlator: 16, 36 -- Thread-Topic: [Microscopy] Re: Disposal of sodium cacodylate 0.1M 16, 36 -- Thread-Index: AcnSnX8G9aSAFydaSj+zwIwZgRKw+AAaafGA 16, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 16, 36 -- To: {jacqueline.williams-at-stjude.org} 16, 36 -- Cc: {microscopy-at-microscopy.com} 16, 36 -- X-OriginalArrivalTime: 12 May 2009 13:55:20.0289 (UTC) FILETIME=[49C9AD10:01C9D309] 16, 36 -- X-Scanned-By: SALK-Content-Filter 16, 36 -- Content-Transfer-Encoding: 8bit 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CDtNPF012854 ==============================End of - Headers==============================
At NIH we send the Sodium Cacodylate Buffer as well as the fixatives made with it (each separately) through our Chemical Waste Disposal Department. I was told when I started using this buffer that the reason is the arsenic.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced. ==
That sounds more like a stereology problem than a morphology one - try Elais, Hans, Hyde, Dallas M. A Guide to Practical Stereology 1983. S.Krager AG
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
-----Original Message----- X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] Sent: Monday, May 11, 2009 1:47 PM To: rjharris-at-uwo.ca
Dear Users, I have a user who wants some morphometric data on his mitochondria. He wants to prove if they are like tubules or round (ball-like) and he needs a good reference source (textbook or article). Basically wants 3d info without having to do reconstructions. Any ideas?
Thanks, M Delannoy
==============================Original Headers============================== 2, 27 -- From delannoy-at-jhmi.edu Mon May 11 12:39:09 2009 2, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4BHd9Wl004741 2, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 May 2009 12:39:09 -0500 2, 27 -- X-IronPort-AV: E=Sophos;i="4.40,329,1238990400"; 2, 27 -- d="scan'208";a="211798483" 2, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 2, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 11 May 2009 13:39:09 -0400 2, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu 2, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 2, 27 -- with ESMTP id {0KJH00BM9QDAZF50-at-jesmail.johnshopkins.edu} for 2, 27 -- microscopy-at-microscopy.com; Mon, 11 May 2009 13:39:10 -0400 (EDT) 2, 27 -- Received: from [10.181.192.192] (Forwarded-For: [10.16.66.59]) 2, 27 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 11 May 2009 13:39:10 -0400 2, 27 -- Date: Mon, 11 May 2009 13:39:10 -0400 2, 27 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 27 -- Subject: morphometry 2, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 27 -- Message-id: {f524aec09dac.4a082a7e-at-johnshopkins.edu} 2, 27 -- MIME-version: 1.0 2, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 2, 27 -- Content-type: text/plain; charset=us-ascii 2, 27 -- Content-language: en 2, 27 -- Content-transfer-encoding: 7BIT 2, 27 -- Content-disposition: inline 2, 27 -- X-Accept-Language: en 2, 27 -- Priority: normal ==============================End of - Headers==============================
==============================Original Headers============================== 12, 29 -- From rjharris-at-uwo.ca Tue May 12 15:01:31 2009 12, 29 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CK1U8a005859 12, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:01:30 -0500 12, 29 -- MIME-version: 1.0 12, 29 -- Content-type: text/plain; charset=iso-8859-1 12, 29 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 12, 29 -- by zeppo.mail.uwo.pri 12, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 12, 29 -- with ESMTP id {0KJJ0071PRMG38G0-at-zeppo.mail.uwo.pri} for 12, 29 -- Microscopy-at-microscopy.com; Tue, 12 May 2009 16:01:28 -0400 (EDT) 12, 29 -- Received: from rjbook 12, 29 -- (CPE001839e498ad-CM0019475d354c.cpe.net.cable.rogers.com [99.249.82.51]) 12, 29 -- by zeppo.mail.uwo.pri 12, 29 -- (Sun Java(tm) System Messaging Server 6.3-7.04 (built Sep 26 2008; 32bit)) 12, 29 -- with ESMTPSA id {0KJJ00I6PRMFYE20-at-zeppo.mail.uwo.pri} for 12, 29 -- Microscopy-at-microscopy.com; Tue, 12 May 2009 16:01:28 -0400 (EDT) 12, 29 -- From: Richard Harris {rjharris-at-uwo.ca} 12, 29 -- To: delannoy-at-jhmi.edu, MSA Listserver {Microscopy-at-microscopy.com} 12, 29 -- References: {200905111746.n4BHku6j017215-at-ns.microscopy.com} 12, 29 -- In-reply-to: {200905111746.n4BHku6j017215-at-ns.microscopy.com} 12, 29 -- Subject: RE: [Microscopy] morphometry 12, 29 -- Date: Tue, 12 May 2009 16:01:28 -0400 12, 29 -- Message-id: {002d01c9d33c$701a9aa0$504fcfe0$-at-ca} 12, 29 -- X-Mailer: Microsoft Office Outlook 12.0 12, 29 -- Content-language: en-us 12, 29 -- Thread-index: AcnSYH1waPPOU5oWR+CKASsms06p2QA24SoA 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CK1U8a005859 ==============================End of - Headers==============================
Microwave Processing Workshop May 20-22, 2009 CBS Imaging Center, University of Minnesota
Several spots have opened up in our upcoming microwave workshop! Click the following link for more info or to register:
http://www.cbs.umn.edu/ic/events/mwws09.shtml
A workshop focusing on new techniques in microwave specimen processing for light and electron microscopy. • In vivo labeling • Principles of microwave fixation for biological materials • Principles of immunolocalization for: • Confocal and fluorescence microscopy • Electron microscopy • In situ hybridization As part of the Workshop we will be using our state-of-the-art wide field and confocal microscope systems.
-- Tracy E. Anderson Microscopist and digital imaging specialist Imaging Center University of Minnesota College of Biological Sciences Phone: 612.624.3454 Fax: 612.624.1799 http://www.cbs.umn.edu/ic/
==============================Original Headers============================== 12, 22 -- From trazy-at-umn.edu Tue May 12 15:26:44 2009 12, 22 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4CKQhe2020819 12, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:26:43 -0500 12, 22 -- Received: from universiy0lfra (x-160-94-173-210.cbs.umn.edu [160.94.173.210]) 12, 22 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP 12, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 12 May 2009 15:26:42 -0500 (CDT) 12, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-210.cbs.umn.edu [160.94.173.210] #+LO+TS+AU+HN 12, 22 -- X-Umn-Classification: local 12, 22 -- From: "Tracy E. Anderson" {trazy-at-umn.edu} 12, 22 -- To: {Microscopy-at-microscopy.com} 12, 22 -- Subject: Microwave Workshop May 20-22 - several spots have just opened up! 12, 22 -- Date: Tue, 12 May 2009 15:26:43 -0500 12, 22 -- Message-ID: {BA7BC89F76E04E78B98DBB41765D8406-at-universiy0lfra} 12, 22 -- MIME-Version: 1.0 12, 22 -- Content-Type: text/plain; 12, 22 -- charset="iso-8859-1" 12, 22 -- X-Mailer: Microsoft Office Outlook 11 12, 22 -- Thread-Index: AcnTP/ZvM0y3VBO+SQuev3JNLuUDjA== 12, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 12, 22 -- Content-Transfer-Encoding: 8bit 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4CKQhe2020819 ==============================End of - Headers==============================
Hi, I was just wondering if anyone out there knew how I could get my hands on Reichert-Jung (Leica) Ultra-cut 701701 manual. I have tried searching for a PDF etc online, but I cant seem to find anything. Any leads would be appreciated. Thank you. Sincerely Amy Albin EM Technician University of Cincinnati Physicians
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Email: schaffne-at-patho.unibe.ch Name: Thomas Schaffner
Organization: Institute of Pathology University of Bern
Title-Subject: [Filtered] Differential optical staining of colorless living Organisms
Question: Hi, I wonder about an elegant technique shown at our Institute about 35 years ago by R(obert) F Smith, then retired photographer at the Brookhaven National Laboratory. He published an article in J. Biol. Phot. Assoc Vol: 23, Nos 2 and 3, pages 74-77; 1955 entitled "Differential optical staining of colorless living organisms in macrophotography", unfortunately unavailable through our libraries. The brilliant colored images of live fish embryos in sharply defined z-planes were apparently obtained with polarized colored light sources generating some kind of interference contrast if I am not mistaken. I now wonder about the physical principles that were involved and how the stereoscope he usaed had to be set up. Any comments are welcome.
This sounds like Rheinberg illumination. Basically, one sets up the microscope for darkfield illumination, and then places colored filters between the light source and the condenser. For example a blue filter before 1/2 of the condenser, and an orange filter before the other 1/2. Any color filters can be used, depending on the desired effect. Darkfield illumination of whole critters (gastrotrichs are really cool) can have a DIC look to them. I haven't seen this used with crossed polarizers, but I don't see why it can't be done.
Phil
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Email: levilr-at-ptd.net Name: LeeLevine
Organization: Process Solutions Consulting
Title-Subject: [Filtered] Gold Sputter Target Available
Question: 100% gold annular target on an aluminum ring , Fits Polaron - BioRad - VG Microtech models E5100,SC515, SC510, SC7610, 82mmOD X 60mmID, 0.1 mm thickness
I seem to remember an interesting article in Microscopy today explaining the advantage of illuminating a sample with one wavelength. One could sequentially illuminate with 3 different wavelengthes and then merge the 3 images to obtain a sharper final image. In my brain of biologist the explanation had something to do with the fact that a light containing different wavelengthes (like in white light) interfere in different planes (although very near). Sorry if my explanation seems confuse, it probably reflects my understanding of the phenomena ;-)
Regards,
Stephane
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==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Wed May 13 08:23:34 2009 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4DDNXFH000584 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 08:23:34 -0500 4, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 24 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n4DDNSWp027364 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 09:23:30 -0400 4, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 24 -- Wed, 13 May 2009 09:23:27 -0400 4, 24 -- Mime-Version: 1.0 4, 24 -- Message-Id: {f06240808c6307a3a167c-at-[141.209.160.249]} 4, 24 -- Date: Wed, 13 May 2009 09:23:24 -0400 4, 24 -- To: Microscopy-at-microscopy.com 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: Re: [Microscopy] viaWWW: optical staining of colorless living 4, 24 -- Organisms 4, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 24 -- X-OriginalArrivalTime: 13 May 2009 13:23:27.0894 (UTC) FILETIME=[00531B60:01C9D3CE] 4, 24 -- X-Canit-CHI2: 0.00 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 24 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 13269997 - 07a5bab390cb 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 24 -- From nizets2-at-yahoo.com Thu May 14 09:56:01 2009 12, 24 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4EEtwrW026724 12, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 09:55:59 -0500 12, 24 -- Received: (qmail 60072 invoked by uid 60001); 14 May 2009 14:55:56 -0000 12, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1242312956; bh=3RJfD8Povpg0DJVJdklASkg/s9yTEUF8HfQbpUQVwsk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=fozIxk6OdXBV6PUyiRNAj8+ch1NgIw3dWXUIzCbPAEGQixj33YjX55scMhwyEUZT9xvb1qv+8+Z8an4MmOcTEkAlTOigdLp91foqDi57u5GpFrOr63qw91P/F7G/j0rQ2vNEDy3g5xjZSIWOcKXvJgNfuv4NyiQ2LNp7rUrnRSc= 12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 24 -- s=s1024; d=yahoo.com; 12, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 12, 24 -- b=slhNGtyryROKrx/+Ehb8TNlTh9clP6vw3opMspqtLfUpy3LwXcwAGniyWNElrqLmT+nYt9SG8Xrp4XqW+E9/VOuLbdhvF1MLm71V75NWfEWdSvHw8bxT9wy6M9amJ2Jj9H+QB5Adv9cb6QEXDmjBOF6oPWYZT5bDDtc/FZ5G/lc=; 12, 24 -- Message-ID: {57259.59281.qm-at-web110801.mail.gq1.yahoo.com} 12, 24 -- X-YMail-OSG: QjN7QqwVM1lXkSAUNK8TehbKYwE_zElXLX7KY8wPgX5alR_WB9cJi7LC7M3cz14X0IHuSbEGHSFdLvi.ezEBgvmFHv4hHnWSXCnyqfEIDc5d2ADpz8T5TPA5DmogTTYxv3CN5DscAFkOOCEYxDgxJQk_8qLPrmWSP0UgyzCByl8XiGVniJEGcEKoSfWKbUEIBFdHzjxNciw0lyxd_pT0qDx69bZY3j59XBmpupvFDsV.cgmw9pI7Gf24yT..GdqpVdN5jgYI9LhJOpCwUDtYFzMSkXzbsPePyvrqRyDaaK14nS5fIISsQ_MCgtjJ9Q.CC4CpFPqLAEc7PGBOjx8ojl6E1e2At4OE4S555P3zrA-- 12, 24 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Thu, 14 May 2009 07:55:55 PDT 12, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10 12, 24 -- References: {200905131337.n4DDb8xW007653-at-ns.microscopy.com} 12, 24 -- Date: Thu, 14 May 2009 07:55:55 -0700 (PDT) 12, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com} 12, 24 -- Subject: Re: [Microscopy] Re: viaWWW: optical staining of colorless living 12, 24 -- To: microscopy-at-microscopy.com 12, 24 -- In-Reply-To: {200905131337.n4DDb8xW007653-at-ns.microscopy.com} 12, 24 -- MIME-Version: 1.0 12, 24 -- Content-Type: text/plain; charset=iso-8859-1 12, 24 -- Content-Transfer-Encoding: 8bit 12, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EEtwrW026724 ==============================End of - Headers==============================
Different objectives have different corrections for chromatic and other aberrations. If a achromatic objective is used, green light has the highest corrections, so red and blue will be a little fuzzy or off set in the image. I suspect, images taken with pure blue light will have the same uncorrected values. So you focus for blue, but that causes a difference in your image. Repeat that with red and the same effects. Combine the images and all the points will not fall on each other.
Use a apochromatic objective, well now you've got focus correction for three wavelengths and spherical correction for two. Combining images should work better, but not better, I suspect, that using white light.
Many microscopes used to come with a green filter (back in the B&W days) because green light has the best correction in achromatic or apochromatic. Substage condensors also played a role in image quality. Us codgers used to wish upon a star, wanting apos instead of achro.
I suspect sharply defined z-planes were the effect of high NA objectives, open substage iris and careful focus.
Stay safe.. Frank
nizets2-at-yahoo.com
05/14/2009 11:15 To AM frank_karl-at-lincolnelectric.com cc
Please respond to Subject nizets2-at-yahoo.com [Microscopy] viaWWW: optical staining of colorless living
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Hi!
I seem to remember an interesting article in Microscopy today explaining the advantage of illuminating a sample with one wavelength. One could sequentially illuminate with 3 different wavelengthes and then merge the 3 images to obtain a sharper final image. In my brain of biologist the explanation had something to do with the fact that a light containing different wavelengthes (like in white light) interfere in different planes (although very near). Sorry if my explanation seems confuse, it probably reflects my understanding of the phenomena ;-)
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} } Email: } Name: Thomas Schaffner } } Organization: Institute of Pathology University of Bern } } Title-Subject: [Filtered] Differential optical staining of colorless } living Organisms } } Question: Hi, I wonder about an elegant technique shown at our } Institute about 35 years ago by R(obert) F Smith, then retired } photographer at the Brookhaven National Laboratory. He published an } article in J. Biol. Phot. Assoc Vol: 23, Nos 2 and 3, pages 74-77; } 1955 entitled "Differential optical staining of colorless living } organisms in macrophotography", unfortunately unavailable through our } libraries. } The brilliant colored images of live fish embryos in sharply defined } z-planes were apparently obtained with polarized colored light } sources generating some kind of interference contrast if I am not } mistaken. } I now wonder about the physical principles that were involved and how } the stereoscope he usaed had to be set up. } Any comments are welcome. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Wed May 13 08:23:34 2009 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4DDNXFH000584 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 08:23:34 -0500 4, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 24 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id n4DDNSWp027364 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 May 2009 09:23:30 -0400 4, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 24 -- Wed, 13 May 2009 09:23:27 -0400 4, 24 -- Mime-Version: 1.0 4, 24 -- Message-Id: {f06240808c6307a3a167c-at-[141.209.160.249]} 4, 24 -- Date: Wed, 13 May 2009 09:23:24 -0400 4, 24 -- To: Microscopy-at-microscopy.com 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: Re: [Microscopy] viaWWW: optical staining of colorless living 4, 24 -- Organisms 4, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 24 -- X-OriginalArrivalTime: 13 May 2009 13:23:27.0894 (UTC) FILETIME= [00531B60:01C9D3CE] 4, 24 -- X-Canit-CHI2: 0.00 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 24 -- X-Spam-Score: -4.40 () [Tag at 5.00] L_EXCH_MF,RDNS_NONE,Bayes (0.0001,-0.5) 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 13269997 - 07a5bab390cb 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 12, 24 -- From nizets2-at-yahoo.com Thu May 14 09:56:01 2009 12, 24 -- Received: from web110801.mail.gq1.yahoo.com (web110801.mail.gq1.yahoo.com [67.195.13.224]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4EEtwrW026724 12, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 09:55:59 -0500 12, 24 -- Received: (qmail 60072 invoked by uid 60001); 14 May 2009 14:55:56 -0000 12, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1242312956; bh=3RJfD8Povpg0DJVJdklASkg/s9yTEUF8HfQbpUQVwsk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=fozIxk6OdXBV6PUyiRNAj8+ch1NgIw3dWXUIzCbPAEGQixj33YjX55scMhwyEUZT9xvb1qv +8 +Z8an4MmOcTEkAlTOigdLp91foqDi57u5GpFrOr63qw91P/F7G/j0rQ2vNEDy3g5xjZSIWOcKXvJgNfuv4NyiQ2LNp7rUrnRSc=
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==============================Original Headers============================== 3, 24 -- From frank_karl-at-lincolnelectric.com Thu May 14 10:44:44 2009 3, 24 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EFihUi011059 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 10:44:44 -0500 3, 24 -- In-Reply-To: {200905141515.n4EFFTI6007474-at-ns.microscopy.com} 3, 24 -- Subject: Re: [Microscopy] viaWWW: optical staining of colorless living 3, 24 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com 3, 24 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 3, 24 -- Message-ID: {OFA188FCE1.597E3BA8-ON852575B6.005465AF-852575B6.00567427-at-lincolnelectric.com} 3, 24 -- Date: Thu, 14 May 2009 11:44:19 -0400 3, 24 -- From: Frank_Karl-at-lincolnelectric.com 3, 24 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 3, 24 -- 07, 2008) at 05/14/2009 11:44:19 AM, 3, 24 -- CD-MIME complete at 05/14/2009 11:44:19 AM, 3, 24 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 3, 24 -- 07, 2008) at 05/14/2009 11:44:19 AM, 3, 24 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 3, 24 -- 07, 2008) at 05/14/2009 11:44:19 AM, 3, 24 -- Serialize complete at 05/14/2009 11:44:19 AM 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="ISO-8859-1" 3, 24 -- Content-Transfer-Encoding: 8bit 3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EFihUi011059 ==============================End of - Headers==============================
We have several pieces of optical equipment that seem to be working but we do not have the manuals for them. If anyone has copies of the manuals for the following equipment please contact me at riba-at-umd.edu .
THORN EMI GENCOM Inc. C-10 Photon counter Acton Research Corporation monochromator Model No. 275 VELMEX 86 mm control/driver
Thanks a lot.
Lourdes Salamanca-Riba
Dr. L. Salamanca-Riba Professor Materials Science and Engineering Dept. University of Maryland College Park, MD 20742 (301) 405-5220 riba-at-umd.edu
==============================Original Headers============================== 9, 22 -- From riba-at-umd.edu Thu May 14 11:12:36 2009 9, 22 -- Received: from md4.mail.umd.edu (md4.mail.umd.edu [128.8.31.174]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EGCZKv026795 9, 22 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 11:12:35 -0500 9, 22 -- Received: from LSalamancaRPC (129-2-175-79.wireless.umd.edu [129.2.175.79]) 9, 22 -- by md4.mail.umd.edu (MOS 3.10.4-GA) 9, 22 -- with ESMTP id AKW34985 (AUTH riba); 9, 22 -- Thu, 14 May 2009 12:12:34 -0400 (EDT) 9, 22 -- From: "Lourdes Salamanca-Riba" {riba-at-umd.edu} 9, 22 -- To: {microscopy-at-microscopy.com} 9, 22 -- Subject: FW: LM Need manuals for equipment 9, 22 -- Date: Thu, 14 May 2009 12:12:20 -0400 9, 22 -- Message-ID: {003201c9d4ae$c42ea8c0$4c8bfa40$-at-edu} 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; 9, 22 -- charset="iso-8859-1" 9, 22 -- X-Mailer: Microsoft Office Outlook 12.0 9, 22 -- Thread-index: AcnUrV+AkOhabKa8TemZgXWldPxEoAAAUZuA 9, 22 -- Content-Language: en-us 9, 22 -- X-Junkmail-Whitelist: YES (by domain whitelist at md4.mail.umd.edu) 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EGCZKv026795 ==============================End of - Headers==============================
I have just inherited a Zeiss Invertoscope ID 03 and have pulled the operating instructions off the web. Unfortunately, I was not able to find any comments from reviewers on the limitations or advantages of this little scope. Does anyone have any experience with this particular model?
Warmest Regards Deb
Debra Moreau, Ph.D. Food Safety & Quality | Salubrité at qualité des ailments Agriculture and Agri-Food Canada | Agriculture et Agroalimentaire Canada 32 Main Street | 32 Rue Main Kentville, Nova Scotia | Nouvelle Écosse B4N 1J5 Debra.Moreau-at-agr.gc.ca {mailto:Debbie.Moreau-at-agr.gc.ca} Telephone | Téléphone 902-679-5710 Facsimile | Télécopieur 902-679-2311 Teletypewriter | Téléimprimeur 613-759-7470 Government of Canada | Gouvernement du Canada
==============================Original Headers============================== 7, 22 -- From Debra.Moreau-at-AGR.GC.CA Thu May 14 11:33:51 2009 7, 22 -- Received: from agrpazsmtp8.agr.gc.ca (agrpazsmtp8.agr.gc.ca [192.197.71.119]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EGXo8n009739 7, 22 -- for {Microscopy-at-Microscopy.com} ; Thu, 14 May 2009 11:33:51 -0500 7, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 22 -- Content-class: urn:content-classes:message 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; 7, 22 -- charset="iso-8859-1" 7, 22 -- Subject: Re: Comments on Zeiss ID 03 7, 22 -- Date: Thu, 14 May 2009 12:33:49 -0400 7, 22 -- Message-ID: {00CAA86962A9F24E84F35531C7CE65DC0111F0F9-at-ONOTTAXMS4.AGR.GC.CA} 7, 22 -- X-MS-Has-Attach: 7, 22 -- X-MS-TNEF-Correlator: 7, 22 -- Thread-Topic: Re: Comments on Zeiss ID 03 7, 22 -- Thread-Index: AcnUscK9DAK13Ja3Qv26980gnMkbcQ== 7, 22 -- From: "Moreau, Debra" {Debra.Moreau-at-AGR.GC.CA} 7, 22 -- To: {Microscopy-at-Microscopy.com} 7, 22 -- X-OriginalArrivalTime: 14 May 2009 16:33:50.0840 (UTC) FILETIME=[C3581780:01C9D4B1] 7, 22 -- Received-SPF: none 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4EGXo8n009739 ==============================End of - Headers==============================
I had a chance to teach on a course with Dr. Smith about that time. I agree with Phil that this is probably Rheinberg Illumination. The method that Phil has described will, indeed, give you one color on one side of a gradient (slope) and a different color on the opposite slope or gradient and that, under the right conditions, those images can appear more 3-dimensional. I also agree that the high NA objective is necessary for optical sectioning. And remember: this approach will be very directional: the gradients need to sit parallel to the filters for optimum effect.
Years ago, Kodak made a set of Rheinberg filters which we used in many many of our classes. When they stopped making them, we acquired the master and, at one time, had them available for a modest cost. Rather than the half-and-half model proposed by Phil, these sets are annular rings which can be used for magnifications up to about 16x. The underlying physics is pretty simple and is based on modifying the diffraction pattern used to form the image. To see a diffraction pattern, use a simple specimen (grating, the dots in the "fins" on pleurasigma angulatum, and, for those of you who do reflected light work, the regular parallel structures in a FinFET from a computer chip). If you are doing the experiment in transmitted light, remove the condenser and just place a pinhole over the light port. Remove the eyepiece and peer down the tube into the back focal plane of the objective (BFPo). Center the image of the pinhole in the middle of the BFPo. Put the sample on the stag! e. (F or reflected light, close the AI as tightly as possible). You should see a pattern of spots. The finer the structure in the sample, the more broadly spaced the spots in the pattern, so you may need play with different objectives in order to capture a sufficient amount of the pattern.
In any event: the central (Zero Order) spot in the diffraction pattern is responsible for the background. All the other diffracted spots are sample dependent and are responsible for carrying the "code" from the sample to the image for spacing, orientation, and edge information. If you color the Zero order, the background in the image will automatically be that color. If you color any of the diffracted orders, the specimen detail will automatically be that color. The simplest Rheinberg filters in the Kodak set have both central spots and rings, so you can make "sandwiches" to suit your sample and yourself. For example, we worked with a major Ketchup manufacture many years ago on an application for counting very thin filamentous mold strands. In normal brightfield, the strands were nearly invisible (soft gray against a white background). However, we provided our class with a set of Rheinbergs. If they used a clear central spot and, say, a turquoise outer ring, the mol! d show ed up a deep turquoise strands against a white background. Or, if they used a red central spot with a clear outer ring, the strands showed up as white against a red background. One combination we have found especially valuable is a blue central spot with a gold outer ring (OK: This is a test: what color was the background and what color was the mold?). For good, rich color, we advocate stacking 2 of each ring.
Similar filters can be made for your individual microscope by setting up Koehler illumination using a 10x or 16x objective, removing the eyepiece, and, while peering into the BFPo, opening the CONDENSER aperture so that the edges just touch the edge of the BFP, then VERY carefully, removing the condenser and measuring the diameter of the opening. That is the size necessary for the central spot. Next, measure the aperture of the condenser, fully open. The difference between the two is the width necessary for the outer ring. Just construct spots and rings from densely colored filter material (ex: available from Edmund Scientific). Stack the colors as you see fit and use a slim piece of tape over the edges to hold them together. To use, just set up Koehler illumination, open the condenser iris completely, and gently tuck the filter into roughly space just about where the iris is.
I've checked to see if we had any of the old sets available, but they appear to be packed away. Will try to unearth them and let you all know if /when they are available again. Rheinberg is an OLD technique (mid 1800's) but great for lots of routine observations and marvelous for teaching.
Hope this was helpful.
Best regards, Barbara Foster, President and Sr. Consultant
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
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: as with darkfield, there is a central region and a ring around it At 08:43 AM 5/13/2009, oshel1pe-at-cmich.edu wrote:
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==============================Original Headers============================== 18, 21 -- From bfostermme-at-sbcglobal.net Thu May 14 16:31:57 2009 18, 21 -- Received: from smtp108.sbc.mail.mud.yahoo.com (smtp108.sbc.mail.mud.yahoo.com [68.142.198.207]) 18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4ELVudB008611 18, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 16:31:57 -0500 18, 21 -- Message-Id: {200905142131.n4ELVudB008611-at-ns.microscopy.com} 18, 21 -- Received: (qmail 17632 invoked from network); 14 May 2009 21:31:56 -0000 18, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 21 -- s=s1024; d=sbcglobal.net; 18, 21 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Mime-Version:Content-Type; 18, 21 -- b=qdHsEP0HCTfeuN1qKtAbNs3tNG7NcMz6oKRn4CxMU/qAY3RZBsSXmCtq0EjMUh9PWO1sqNS68tF4ZOw6vTH0dxHXj+E9z3ayHdzWLRRMKRdsIyjRkK1QSXLdMB9UoYOBQdaHs6AGYPxi08/+H60HwsmaZHOzDE8FUs2Bzxvju5w= ; 18, 21 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-99.168.106.177 with login) 18, 21 -- by smtp108.sbc.mail.mud.yahoo.com with SMTP; 14 May 2009 21:31:56 -0000 18, 21 -- X-YMail-OSG: F4RacukVM1nVsm5_WNB1Ad9_NsXNUCI5i98fxbH3TF92nbLTn.87ihOcMM8qbtbY8x0tcQw8xP9g1GUlloCvAVUcWVHQkv5b.YF.o20szPeXRsh3B.8kO4F6shj466gw84IZHa4H2x5m5NPUnPD3694b2yAP0.8NYCsw1it2sv4GnBWC9mt.ssHWdDa_dWicKdobQcFgjbzA3sTyt07UThoVcOChi5zvFXPnwcIkaRpp1CBSTLLdrpiycSlkrqgOiYAUoiZqmDaKQdiUHp6H.y2svywsP9piLtqnxcrtXtW3w1HP1dp33.ljWRDzz90jqYuSa6fqgt.dzp4QHK6JqEeohzs- 18, 21 -- X-Yahoo-Newman-Property: ymail-3 18, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 18, 21 -- Date: Thu, 14 May 2009 16:25:56 -0500 18, 21 -- To: microscopy-at-microscopy.com 18, 21 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 18, 21 -- Subject: viaWWW: optical staining of colorless 18, 21 -- Mime-Version: 1.0 18, 21 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Dear Group: does anyone have any idea who I can contact about repairs for this model? Thanks so much. Barbara
==============================Original Headers============================== 1, 33 -- From maloneyb-at-fiu.edu Thu May 14 17:24:24 2009 1, 33 -- Received: from sodium.colo.stayonline.net (potassium.colo.stayonline.net [69.25.86.41]) 1, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4EMON1A025160 1, 33 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 17:24:24 -0500 1, 33 -- X-ASG-Debug-ID: 1242310383-3287001c0000-4CH8be 1, 33 -- X-Barracuda-URL: http://192.168.5.11:8000/cgi-bin/mark.cgi 1, 33 -- Received: from et-den-37.site.stayonline.net (unknown [12.69.19.194]) 1, 33 -- by sodium.colo.stayonline.net (Spam Firewall) with ESMTP id ADA784557F7 1, 33 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 10:13:03 -0400 (EDT) 1, 33 -- Received: from et-den-37.site.stayonline.net ([12.69.19.194]) by sodium.colo.stayonline.net with ESMTP id JnKaL0xgmqpETH08 for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 10:13:03 -0400 (EDT) 1, 33 -- Received: from [192.168.50.99] ([192.168.50.99]) 1, 33 -- by et-den-37.site.stayonline.net (8.13.8/8.12.6) with ESMTP id n4EED2T3057990 1, 33 -- for {microscopy-at-microscopy.com} ; Thu, 14 May 2009 14:13:03 GMT 1, 33 -- Message-ID: {4A0C26E6.4020105-at-fiu.edu} 1, 33 -- Disposition-Notification-To: Barbara {maloneyb-at-fiu.edu} 1, 33 -- Date: Thu, 14 May 2009 10:12:54 -0400 1, 33 -- From: Barbara {maloneyb-at-fiu.edu} 1, 33 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 1, 33 -- MIME-Version: 1.0 1, 33 -- To: microscopy-at-microscopy.com 1, 33 -- X-ASG-Orig-Subj: Reichert-Jung Model 250 microtome 1, 33 -- Subject: Reichert-Jung Model 250 microtome 1, 33 -- X-Priority: 2 (High) 1, 33 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 33 -- Content-Transfer-Encoding: 7bit 1, 33 -- X-Barracuda-Connect: UNKNOWN[12.69.19.194] 1, 33 -- X-Barracuda-Start-Time: 1242310384 1, 33 -- X-Barracuda-Spam-Score: 0.00 1, 33 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests= 1, 33 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.1.25799 1, 33 -- Rule breakdown below 1, 33 -- pts rule name description 1, 33 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
From wa1hco-at-adelphia.net Thu May 14 21:57:16 2009 Return-Path: {wa1hco-at-adelphia.net} Received: from google.com (198.subnet125-161-87.speedy.telkom.net.id [125.161.87.198] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4F2vElB016747 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 14 May 2009 21:57:15 -0500 Received: from [124.116.22.183] (HELO google.com) by luckybulls.cn; Fri, 15 May 2009 09:57:12 +0700
Multi-year service agreement needed on our electron microscopes.
Bids will be opened and contract awarded June 3rd.
Bid package is located at the following URL: http://www2.winthrop.edu/procurement/bids.htm
Microscopes are: ISI super IIIA with EDS Zeiss EM10C with top-entry gonio Both have been continuously under contract since installation here
Please direct any questions initially through Teresia Sexton at:
Teresia C. Sexton Procurement Officer Winthrop University Purchasing Services & Risk Management 307 Tillman Hall Rock Hill, SC 29733 Telephone: 803-323-2143, ext. 6026 Fax: 803-323-2564 sextont-at-winthrop.edu
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
==============================Original Headers============================== 11, 20 -- From smithj-at-winthrop.edu Fri May 15 08:25:23 2009 11, 20 -- Received: from messenger.winthrop.edu (messenger.winthrop.edu [199.79.254.177]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4FDPK1t026307 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 15 May 2009 08:25:21 -0500 11, 20 -- Received: from berlin.win.winthrop.edu (berlin.win.winthrop.edu [10.2.0.22]) 11, 20 -- by messenger.winthrop.edu (MOS 3.8.6-GA) 11, 20 -- with ESMTP id GTA18783; 11, 20 -- Fri, 15 May 2009 09:25:16 -0400 (EDT) 11, 20 -- Received: from Julian-Smiths-Computer.local ([10.3.84.33]) by berlin.win.winthrop.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 20 -- Fri, 15 May 2009 09:25:15 -0400 11, 20 -- Message-ID: {4A0D6D3B.7020003-at-winthrop.edu} 11, 20 -- Date: Fri, 15 May 2009 09:25:15 -0400 11, 20 -- From: Julian Smith III {smithj-at-winthrop.edu} 11, 20 -- User-Agent: Thunderbird 2.0.0.21 (Macintosh/20090302) 11, 20 -- MIME-Version: 1.0 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- Subject: Service contract for SEM and TEM needed in Southeastern US 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-OriginalArrivalTime: 15 May 2009 13:25:15.0685 (UTC) FILETIME=[95663550:01C9D560] ==============================End of - Headers==============================
From olkpvimpg_rhc-at-emapfrance.com Fri May 15 23:49:34 2009 Return-Path: {olkpvimpg_rhc-at-emapfrance.com} Received: from google.com (p5210-ipad08morioka.iwate.ocn.ne.jp [60.37.120.210]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4G4nWUN014867 for {microscopylistserverarchive-at-microscopy.com} ; Fri, 15 May 2009 23:49:33 -0500 Received: from [98.82.46.182] (HELO google.com) by thinycleansneak.org; Sat, 16 May 2009 14:19:41 +0930
Hello all,
I need your critique on CL options available, overall, and at this specific moment I need info on using CL for Geological applications (zircons, etc).
I have some info from Gatan and have come across the CL option from KE Development as well.
Are there any others out there, how well does the one you are using work for EM and uProbe applications? (be sure to tell me your CL MFG and model, apps, and spectral range to allow me to insure that I know how to match up requirments now and in the future) Pros / Cons, etc.
You may contact me off list, I need to make the best decision possible for a customer and do not want to steer them wrong due to pricing or poor judgement.
I want to thank everyone who helped with suggestions and ideas about the source of my wavy SEM images. I was very impressed the response from the JEOL community who called and e-mailed me.
Thanks everyone!
The problem was traced to a defective pump on the water chiller. I suspected the motor, but I was wrong. It was the pump.
Stay safe........... Frank
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 7, 21 -- From frank_karl-at-lincolnelectric.com Mon May 18 07:29:57 2009 7, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4ICTvnW032237 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 18 May 2009 07:29:57 -0500 7, 21 -- Subject: Wavy SEM images- resolved 7, 21 -- To: Microscopy-at-microscopy.com 7, 21 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 7, 21 -- Message-ID: {OF0CE82389.F4641E40-ON852575BA.0043E32B-852575BA.00449F96-at-lincolnelectric.com} 7, 21 -- Date: Mon, 18 May 2009 08:29:45 -0400 7, 21 -- From: Frank_Karl-at-lincolnelectric.com 7, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 7, 21 -- 07, 2008) at 05/18/2009 08:29:47 AM, 7, 21 -- CD-MIME complete at 05/18/2009 08:29:47 AM, 7, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 7, 21 -- 07, 2008) at 05/18/2009 08:29:47 AM, 7, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 7, 21 -- 07, 2008) at 05/18/2009 08:29:47 AM, 7, 21 -- Serialize complete at 05/18/2009 08:29:47 AM 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
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Title-Subject: [Filtered] Old Cambridge Stereoscan 250
Question: Dear all,
We have old SEM Cambridge Stereioscan for sale. System is in reasonably good condition though turbomolecular pump controller required to be fixed. Sysytem has digital image aquisition with SE (E-T) and BSE (solid state)detectors as well as EDS detector (thin Be-window) attached for elemental analysis (Na and higher at. #), line scan and x-ray mapping.
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Email: eric.roth-at-med.nyu.edu Name: Eric W. Roth
Organization: NYU
Title-Subject: [Filtered] Myelin problem
Question: Hello list,
We are having a problem with getting very low contrast and precipitation in the myelin of some cultured Neuron cells.
Here is the protocol that we are using for processing: rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M sucrose at room temperature for 30min, then 4C overnight. After rinse 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.
The sample was sectioned at 100nm and imaged at 120kV. Here is a link to download a couple good examples of our problem.
I would advise to make a search on the subject because it is not the first question about myelin embedding. You could try "microscopy listserver myelin" in google.
I have no experience with myelin embedding but I can tell you that phosphate ions precipitate in presence of Ca2+ and most biological tissues contain Ca2+. This may be the reason of the precipitates. You could try Hepes buffer or the very classical cacodylate. To increase the contrast you could try potassium ferrocyanate in osmium tetroxyde. I wrote about it some time about in microscopy today. Just search the listserver. It may well all be the contrasting solutions, I don't know how experienced you or your lab are with EM techniques. Lead citrate can precipitate pretty easily.
Best regards,
Stephane (didn't look at the pics)
----- Original Message ---- X-from: "eric.roth-at-med.nyu.edu" {eric.roth-at-med.nyu.edu} To: nizets2-at-yahoo.com Sent: Wednesday, May 20, 2009 2:19:47 AM
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Email: eric.roth-at-med.nyu.edu Name: Eric W. Roth
Organization: NYU
Title-Subject: [Filtered] Myelin problem
Question: Hello list,
We are having a problem with getting very low contrast and precipitation in the myelin of some cultured Neuron cells.
Here is the protocol that we are using for processing: rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M sucrose at room temperature for 30min, then 4C overnight. After rinse 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.
The sample was sectioned at 100nm and imaged at 120kV. Here is a link to download a couple good examples of our problem.
(unfortunately, my original mailing from the MSA-spam-filter was rejected for the following reason(s): Content-Type: multipart/alternative
This was due to unintendingly having changed the original text-format to Rich format. I apologize for any inconveniencies this might have induced) ----------------------------------------------------------------------
Good morning, hello } Big Apple {, dear Eric,
not to post a nasty/unpleasant comment about your problem..... Have seen your nonetheless nice pic's and have seen that they are completely free of any precipitates, so the problem IMhO for the most part ( 90-99% ) doesn't cohere with your staining solutions.
I bet that you can improve the ultrastructural preservation of myelin sheaths if you shorten your initial fixiation to 15 - 30 [at the most 60!] min's at room temperature or say 15-30 mins at RT, + additional 30 mins -at- 4 degr.C.. From my experience I think that your fixation /fixative over night (despite at 4 degr.C) is "elutive" to/with your delicate cultured neuron cells (and if you check the images you can find some minor preserved membranes [==} organelles], as this perhaps also is true for the speckled appearance of the myelin lamellae). Maybe also a consequence of too rapid dehydration (or ddH2O after osmication). Unfortunately you did not comment on the duration and steps of your dehydration schedule.
Your using PO4 buffer within / for your fixative does not mean necessarily (IMhO) that you "precipitate away" all your Ca++ in your cells, otherwise nobody would use such a fixative any longer for research or diagnostic issues (but I confess that perhaps sodium-cacodylate or HEPES, as suggested by Stepane, would be a promising - also more expensive - alternative.... But: in my opinion you have to think also about the "elutivity" of buffers and fixatives in general, whereby } elutivity { is meant on a molecular scale for more/less hydrophilic substrata for the fixative used and consequences arising afterwards in the dehydration steps).
The preservation of myelin sheaths in most of the diagnostic probes in our lab, as I remember, has always been sufficient, nothing bad is to be said about use of PO4 as the (fix,washing, postfix-) buffer system.
Ca++ and its (biological) complexes (as most PO4-ions) will be precipitated in the higher alcohols (so if you start with e.g. buffer washes[ PO4] after GA-fixation only or additional postfixation by OsO4, and change into / start dehydration with EtOH 50%, then 70% 80,90,6,100% etc. ) you rarely will get Ca++ and or PO4-precipitates (cf. "salt & pepper") in your specimens.
A good choice for the preservation of myelin sheaths (because it's relatively easy to include in the standard processing) would be - after osmication as usual - then washing twice in buffer, then EtOH 50% (for cultured cells) max 2-5 min - then incubation in 1% para-phenylenediamine (PPD) in 70%EtOH (e.g. ca. 15-20 min -at-RT), washing once or twice in pure EtOH 70% and proceed with your standard dehydration steps .... this will retain lipids and glycoconjugates within the spec's (if you would like to read/hear more about that, please reply for the details, information and lit.ref. ).
Best wishes and good luck,
Wolfgang MUSS EM-Lab, Pathology SALK-PMU (Fed. & City Gen. Hosp.) SALZBURG AUSTRIA-EUROPE
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
-----Ursprüngliche Nachricht----- Von: eric.roth-at-med.nyu.edu [mailto:eric.roth-at-med.nyu.edu] Gesendet: Mittwoch, 20. Mai 2009 02:19 An: Muß Wolfgang Betreff: [Microscopy] Myelin problem
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Hello list, We are having a problem with getting very low contrast and precipitation in the myelin of some cultured Neuron cells. Here is the protocol that we are using for processing:
rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M sucrose at room temperature for 30min, then 4C overnight. After rinse 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.
The sample was sectioned at 100nm and imaged at 120kV. Here is a link to download a couple good examples of our problem.
Are you seriously stirring this up again? Didn't we just handle O2 displacement via nitrogen boil off a few weeks ago?
The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a tiny room for a scope and all the N2 would have to be released rapidly enough to displace the O2 in the room (or reduce it from 22% to below 12% for this to become an issue let alone life threatening) without the N2 leaking out the doorway or any O2 leaking in. But in fact it sounds like ". . .going through nitrogen gas like there is no tomorrow." is probably days not minutes, and the scope room is a "normal room" and not a sealed environmental chamber.
I have worked with compressed atmospheric air. No matter how dry it is an extremely corrosive material, degrading fittings, seals and regulator parts. I would suggest the long term costs and safety make working with compressed air a far worse choice than ppN2
On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Elgin; } My I suggest also that you have created a significant safety hazard by } running your air table, and I presume your valving, on nitrogen. The } leak you now have (and everything develops leaks eventually) is } filling up your room with pure nitrogen and displacing the oxygen. You } should be using only clean dry air for these items so that when it } leaks, the room fills up with.....air! Air that you can breath! } Nitrogen should only be used for venting the chamber. } } } Sent from my iPhone. } John Mardinly } } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote: } } } } } } } } } --- } } --- } } -------------------------------------------------------------------- } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Wed May 20 07:53:35 2009 10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KCrZNA004846 10, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 20 May 2009 07:53:35 -0500 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrsGt014924; 10, 25 -- Wed, 20 May 2009 08:53:54 -0400 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrYF2021966; 10, 25 -- Wed, 20 May 2009 08:53:34 -0400 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: microscopy-at-Microscopy.com 10, 25 -- Date: Wed, 20 May 2009 08:53:34 -0400 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak 10, 25 -- CC: "jmardinly-at-gmail.com" {jmardinly-at-gmail.com} 10, 25 -- Message-ID: {4A13C50E.27113.A79CE8A-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200905082112.n48LCJEb013069-at-ns.microscopy.com} 10, 25 -- References: {200905082112.n48LCJEb013069-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
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Email: henrik.kaker-at-guest.arnes.si Name: Henrik Kaker
Organization: Metal Ravne, Slovenia
Title-Subject: [Filtered] Monitor for JEOL JSM 35CF
Question: Dear All,
We are looking for JEOL JSM 35CF monitor(not 35C type). We have a truble with the current monitor and service can't repair the error.
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Email: gill.dealtry-at-nmmu.ac.za Name: Dr Gill Dealtry
Organization: Nelson Mandela Metropolitan University
Title-Subject: [Filtered] frozen samples for LM
Question: I have access to unfixed tissue pieces that were dissected from sacrificed rats, snap frozen within cryotubes in liquid nitrogen and stored at -80 for biochemical analysis. Is it possible to use this for cryo-sections for LM? How would I retrieve the tissue and process it for cryostat sections?
This looks amazingly similar to a problem we had several years ago that resisted any and all attempts to fix it until we began using 2-mercaptoethanol in our protocols. Go to http://www.emc.missouri.edu/pandp.htm to see our procedures. After nearly two years of trying everything else we could think of, this is the only thing that fixed the problem, and the problem has stayed fixed since. Unless, that is, we try processing without 2-Me again.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
-----Original Message----- X-from: eric.roth-at-med.nyu.edu [mailto:eric.roth-at-med.nyu.edu] Sent: Tuesday, May 19, 2009 7:15 PM To: Tindall, Randy D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both eric.roth-at-med.nyu.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: eric.roth-at-med.nyu.edu Name: Eric W. Roth
Organization: NYU
Title-Subject: [Filtered] Myelin problem
Question: Hello list,
We are having a problem with getting very low contrast and precipitation in the myelin of some cultured Neuron cells.
Here is the protocol that we are using for processing: rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M sucrose at room temperature for 30min, then 4C overnight. After rinse 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112.
The sample was sectioned at 100nm and imaged at 120kV. Here is a link to download a couple good examples of our problem.
Hi Eric, Regarding your precipitate problem, I'm not sure what the cause is though pretty certain it's osmium-related as I've had similar problems recently (not neuronal cells though) and it doesn't happen in non-osmicated samples and it is not buffer-dependent. It certainly does not look like a lead citrate problem as this, as you may well know, has the appearance of quite large spherical dense spots all over the sections (caused if you breathe on the solution whilst staining, thus creating Pb carbonate I believe). Anyway, I think I can help you get rid of it though, which may be good news for you. Prepare a saturated aqueous solution of sodium metaperiodate and place it in an ultrasonic bath for a while (15-30min) so that when you shake it up a little it has a milky appearance. Pipette from this milky region (avoiding the undissolved granules at the bottom) and place 20ul drops on parafilm. Slide your grids in very gently from the side of the drops to fully immerse (float them section side down if they're collected on support grids though....and for the subsequent rinses). Leave for 30min and then rinse by immersion in distilled water for 4 X 3min minimum or so. When you do this, again slide the grids in and out of the water very gently and at right angles to the water surface, otherwise you could lose your sections. after this you can stain as normal, perhaps taking on board the advice of some others on here about contrast improvement. This normally works for me and hope it does for you! Cheers, Jules
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
--On 19 May 2009 19:24 -0500 eric.roth-at-med.nyu.edu wrote:
} } } } ------------------------------------------------------------------------- } --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------- } --- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ------------------------------------------------------------------------- } -- Remember this posting is most likely not from a Subscriber, so when } replying please copy both eric.roth-at-med.nyu.edu as well as the } MIcroscopy Listserver } ------------------------------------------------------------------------- } -- } } Email: eric.roth-at-med.nyu.edu } Name: Eric W. Roth } } Organization: NYU } } Title-Subject: [Filtered] Myelin problem } } Question: Hello list, } } We are having a problem with getting very low contrast and } precipitation in the myelin of some cultured Neuron cells. } } Here is the protocol that we are using for processing: } rinse the cells in 0.1M PO4, pH 7.0 for 3 times, and fixed in the } fixative containing 2% Glutaraldehyde, 0.05M PO4, pH7.0 and 0.1M } sucrose at room temperature for 30min, then 4C overnight. After rinse } 3x15min with 0.1M PO4, the cover slips were incubated in 2% OsO4 in } 0.1M PO4 for 1hr, wash with ddH2O and embedded in LX 112. } } The sample was sectioned at 100nm and imaged at 120kV. } Here is a link to download a couple good examples of our problem. } } http://saturn.med.nyu.edu/~ewroth/Neuron_examples.zip } } Any ideas would be greatly appreciated. Thanks! } Eric } } Login Host: 216.165.126.103 } ------------------------------------------------------------------------- } -- } ==============================Original } Headers============================== 11, 11 -- From } zaluzec-at-microscopy.com Tue May 19 19:14:01 2009 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) 11, 11 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id n4K0E1xN031150 11, 11 -- for } {microscopy-at-microscopy.com} ; Tue, 19 May 2009 19:14:01 -0500 11, 11 -- } Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240803c638fbb519f9-at-[206.69.208.22]} } 11, 11 -- Date: Tue, 19 May 2009 19:14:00 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: eric.roth-at-med.nyu.edu (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: Myelin problem } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 23 -- From bafg3-at-sussex.ac.uk Wed May 20 09:51:43 2009 9, 23 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KEpg0U001612 9, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 09:51:42 -0500 9, 23 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:3827) 9, 23 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64) 9, 23 -- (envelope-from {bafg3-at-sussex.ac.uk} ) 9, 23 -- id KJY6M4-000DA9-KB; Wed, 20 May 2009 15:51:40 +0100 9, 23 -- Date: Wed, 20 May 2009 15:51:39 +0100 9, 23 -- To: eric.roth-at-med.nyu.edu, Microscopy-at-microscopy.com 9, 23 -- Subject: Re: [Microscopy] viaWWW: Myelin problem 9, 23 -- Message-ID: {1589150928.1242834699-at-ls0130.lifesci.susx.ac.uk} 9, 23 -- In-Reply-To: {200905200024.n4K0O9iJ011788-at-ns.microscopy.com} 9, 23 -- Originator-Info: login-token=Mulberry:01AM9vm2Iw1QI0D8/1aTMuQuQOdDTawmEqLkTa; 9, 23 -- token_authority=postmaster-at-central.susx.ac.uk 9, 23 -- X-Mailer: Mulberry/2.0.8 (Win32) 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=us-ascii; format=flowed 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- Content-Disposition: inline 9, 23 -- X-Sussex: true 9, 23 -- X-Sussex-transport: remote_smtp_rew 9, 23 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk} ==============================End of - Headers==============================
I neglected to point out in my previous post that our precipitate was osmium (Dr. John Bozzola's lab helped us out with EDS and confirmed this). The 2-Me works by reducing the osmium. We never found out why the problem suddenly started happening, which is frustrating, but it certainly cured it.
Also, the problem happened with many types of tissue.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 7, 27 -- From TindallR-at-missouri.edu Wed May 20 09:58:14 2009 7, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KEwCrl007422 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 09:58:13 -0500 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: ApoEANq3E0rRauUp/2dsb2JhbAC+YAEJiACIToJTgTUF 7, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 7, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 May 2009 09:58:00 -0500 7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Wed, 20 May 2009 09:58:00 -0500 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Subject: Re: myelin problem 7, 27 -- Date: Wed, 20 May 2009 09:58:00 -0500 7, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD80F4-at-UM-XMAIL08.um.umsystem.edu} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Re: myelin problem 7, 27 -- Thread-Index: AcnZW14mXa03oh+GRkqYHT8zEAHqZw== 7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 20 May 2009 14:58:00.0657 (UTC) FILETIME=[5E724C10:01C9D95B] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4KEwCrl007422 ==============================End of - Headers==============================
Hi Eric, Regarding your precipitate problem, I'm not sure what the cause is though pretty certain it's osmium-related as I've had similar problems recently (not neuronal cells though) and it doesn't happen in non-osmicated samples and it is not buffer-dependent. It certainly does not look like a lead citrate problem as this, as you may well know, has the appearance of quite large spherical dense spots all over the sections (caused if you breathe on the solution whilst staining, thus creating Pb carbonate I believe). Anyway, I think I can help you get rid of it though, which may be good news for you. Prepare a saturated aqueous solution of sodium metaperiodate and place it in an ultrasonic bath for a while (15-30min) so that when you shake it up a little it has a milky appearance. Pipette from this milky region (avoiding the undissolved granules at the bottom) and place 20ul drops on parafilm. Slide your grids in very gently from the side of the drops to fully immerse (float them section side down if they're collected on support grids though....and for the subsequent rinses). Leave for 30min and then rinse by immersion in distilled water for 4 X 3min minimum or so. When you do this, again slide the grids in and out of the water very gently and at right angles to the water surface, otherwise you could lose your sections. after this you can stain as normal, perhaps taking on board the advice of some others on here about contrast improvement. This normally works for me and hope it does for you! Cheers, Jules
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
You can search the 2009 program using keywords in the following fields
Symposium, Author& Affiliation, Paper Title
Go to http://www.microscopy.org Select the link to the Annual Meeting (12th left hand button) Select the link to the Search Engine (2nd left hand button)
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 10, 11 -- From zaluzec-at-microscopy.com Wed May 20 10:08:13 2009 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KF8CQg006962 10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 20 May 2009 10:08:12 -0500 10, 11 -- Mime-Version: 1.0 10, 11 -- Message-Id: {p06240803c637a29e370d-at-[206.69.208.22]} 10, 11 -- Date: Wed, 20 May 2009 10:08:11 -0500 10, 11 -- To: microscopy-at-microscopy.com 10, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 10, 11 -- Subject: MM2009 Search Engine now On-Line 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We use an Balzers/Baltec MED 010 to carbon coat our samples for TEM and SEM. It uses carbon fiber thread for one shot flash evaporation (Baltec Ref : LZ 02307 VN, 25 m length).
The french supplier we had for this carbon thread (which comes from Baltec Lichetensten) has no stock, and needs allways 4 to more than 6 weeks for the delivery. So I've two sets of questions, one for users and one for suppliers.
-For users : First what kind of other brand fiber can be used on this device. And secondly, I suppose it should exist a way to treat general purpose carbon fiber , which is generally insolating, to get it conducting, and have a cheaper way than the expensive genuine part (up to } 200. euro for 25 meter !).
-For supplier : the same question about what other brand of fiber could be used. That supplied by Baltec is very thin, less then 1 mm in diameter. I had once some from a well known europeen TEM/SEM accessories supplier which was a real rope, nice for EPMA at high current, but much too thick for the power supply, and giving too to thick films for TEM or SEM imaging. -For europeen supplier : Who would have the Baltec referenced fiber on stock ? And at which price !!! (off list ?!)
Thanks to all for help and answers.
Jacques
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
There is nothing magical about carbon fiber to make it conductive. The conductivity will depend to some extent on how the elementary fibers are formed and the overall cross-sectional area, roughly proportional to the diameter, but variable due to the type of assembly (rope laid, braided, etc.) As far as I can tell, the variety of carbon strings and suitability for particular instruments is related to the electronics that are used to heat them, and it seems that most systems have particular set voltages and power capacities and need a particular fiber characteristic to match the equipment. For example, if a braid that is larger dia and lower resistance is used in place of the smaller dia braid that a power supply is designed to use, the power supply may put out all of its current, maybe even too much, with associated damage, and not have enough power to heat the braid to the sublimation temperature.
The size of the braid, power supply characteristics and physical geometry of the evaporation source to sample are set by the manufacturer to give the predictable result. The manufacturer's quality control is certainly set to maintain this predictable result with their equipment.
Having said this as if there is no choice in the matter, I can say that we use carbon braid in systems designed for carbon rods and since these older systems typically have a 1kW power transformer and a variable autotransformer to drive the power transformer, it is possible to tune the voltage of these systems for many types of carbon "string" (string, yarn, braid, cord....) and also choose the way in which it sublimes; multiple pulses to white hot until it no longer conducts rather than "flash" evaporation is my choice. In an old Kinney Evaporator I use the tungsten basket/boat holders with a 1.25cm insulating spacer and clamp a short piece of carbon string (SPI #11433) between. Using ~30% setting of the autotransformer (gives 30% of the 24VAC secondary to power the heating) I evaporate a single piece of carbon at ~5cm onto mica to make pure carbon films.
I have collected a bit of data, have SEM pictures of the "burned out" string tips (to show they don't explode but sublime away to an open circuit) and some Voltage-current data for my setup. Since I can't make attachments to the list, I will make the offer to share this info and if anyone is further interested I would be happy to send or post on a website.
There is a notable paper on this topic although it is unfortunate that very little information was given on the power supply details even though this was otherwise a very technical report:
Precise and reproducible deposition of thin and ultra-thin carbon films by flash evaporation of carbon yarn in high vacuum. Klaus-Ruediger Peters. J. Micros. (133), p 17. 1984.
Dale Callaham
jacques.faerber-at-ipcms.u-strasbg.fr wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } We use an Balzers/Baltec MED 010 to carbon coat our samples for TEM and } SEM. It uses carbon fiber thread for one shot flash evaporation (Baltec } Ref : LZ 02307 VN, 25 m length). } } The french supplier we had for this carbon thread (which comes from } Baltec Lichetensten) has no stock, and needs allways 4 to more than 6 } weeks for the delivery. So I've two sets of questions, one for users and } one for suppliers. } } -For users : First what kind of other brand fiber can be used on } this device. And secondly, I suppose it should exist a way to treat } general purpose carbon fiber , which is generally insolating, to get it } conducting, and have a cheaper way than the expensive genuine part (up } to } 200. euro for 25 meter !). } } -For supplier : the same question about what other brand of fiber } could be used. That supplied by Baltec is very thin, less then 1 mm in } diameter. I had once some from a well known europeen TEM/SEM accessories } supplier which was a real rope, nice for EPMA at high current, but much } too thick for the power supply, and giving too to thick films for TEM or } SEM imaging. } -For europeen supplier : Who would have the Baltec referenced fiber } on stock ? And at which price !!! (off list ?!) } } Thanks to all for help and answers. } } Jacques }
==============================Original Headers============================== 9, 22 -- From dac-at-research.umass.edu Wed May 20 12:57:40 2009 9, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KHveo6016937 9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 12:57:40 -0500 9, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 9, 22 -- (authenticated bits=0) 9, 22 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n4KHvd0B004970 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 20 May 2009 13:57:40 -0400 9, 22 -- Message-ID: {4A144498.6080107-at-research.umass.edu} 9, 22 -- Date: Wed, 20 May 2009 13:57:44 -0400 9, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 22 -- Reply-To: dac-at-research.umass.edu 9, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16 9, 22 -- MIME-Version: 1.0 9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 22 -- Subject: Re: [Microscopy] Carbon fiber for Baltec evaporator 9, 22 -- References: {200905201602.n4KG2DJt004548-at-ns.microscopy.com} 9, 22 -- In-Reply-To: {200905201602.n4KG2DJt004548-at-ns.microscopy.com} 9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tina.williams-at-ars.usda.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: U.S. Dept. of Agriculture
Title-Subject: [Microscopy] cryo-ultramicrotome access or cryo-unit attachment
Question: Hello,
Does anyone have an FS-1000 cryo attachment that they are using for the RMC MT-6000 XL? If you have one you no longer use, we would like to borrow it or buy one if possible. We need to do trial cryo-ultramicrotome of a sample to see if it will work.
Suppose you have two pieces of silicon, doped as follows: p-doped, conductivity/resistivity in the range 1-25 ohm-cm n-doped, conductivity/resistivity in the range 1-25 ohm-cm
and coated as follows: 50-nm film of Aluminum, etched to form a nm-scale pattern.
Would you expect both specimens to image well in SEM at high magnification (10kx-100kx) without charging artifacts?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 6, 23 -- From donc-at-asmicro.com Thu May 21 08:36:37 2009 6, 23 -- Received: from smtp117.sbc.mail.re3.yahoo.com (smtp117.sbc.mail.re3.yahoo.com [66.196.96.90]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4LDaZb2002864 6, 23 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2009 08:36:37 -0500 6, 23 -- Received: (qmail 52350 invoked from network); 21 May 2009 13:36:34 -0000 6, 23 -- Received: from unknown (HELO asm15) (donc-at-66.178.233.250 with login) 6, 23 -- by smtp117.sbc.mail.re3.yahoo.com with SMTP; 21 May 2009 13:36:33 -0000 6, 23 -- X-Yahoo-Newman-Property: ymail-3 6, 23 -- Message-ID: {8B3BF7CB1B524E89959B04D3CEB476E4-at-asm15} 6, 23 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 6, 23 -- To: "Microscopy List" {microscopy-at-microscopy.com} 6, 23 -- Subject: SEM of silicon - question about polarity of doping 6, 23 -- Date: Thu, 21 May 2009 09:34:02 -0400 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- format=flowed; 6, 23 -- charset="iso-8859-1"; 6, 23 -- reply-type=original 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-Priority: 3 6, 23 -- X-MSMail-Priority: Normal 6, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 6, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
1. The Nanoprobe Network is hosting the third on-line Live Forum on *Friday, May 22 from 11:30 AM to 1:00 PM, EDT. The discussion topic will be “Piezoresponse Force Microscopy”. You are invited to post comments on the Nanoprobe Network Forum anytime prior to, during, or after the event (http://nanoprobenetwork.org/forum).
During the Live Forum, AFM experts from around the globe will discuss issues, ideas, and questions regarding the applications, principles, recent advances and technical know-how of piezoresponse force microscopy of ferroelectrics, multiferroics, and biological systems. The questions you will be able to ask can range from:
* how can I implement PFM on a microsope * how do I distinguish the PFM image from cross-talk * how to acquire high-resolution spectroscopic data , and how to interpret it * what are the advantages and pitfalls of resonance enhanced PFM * can PFM be done in liquid * and many others.
Special expert guests for this chat will be Dr. Sergei Kalinin from Oak Ridge National Laboratory, Dr. Andrei Kholkin from the University of Aveiro, Portugal, and Dr. Alexei Gruverman from the University of Nebraska.
(http://nanoprobenetwork.org/bbpress/forum.php?id=26) This text-based forum allows any Nanoprobe Network member to ask and answer questions, propose ideas, suggest literature, and explore any topic of interest related to PFM. To participate, you must be a member of the Nanoprobe Network. Registration is free. Simply go to http://www.nanoprobenetwork.org, and click on the "Register" button in the upper right.
We hope you find the Nanoprobe Network resource beneficial to your work and we look forward to seeing you on-line!
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 12, 28 -- From sergei2-at-ornl.gov Thu May 21 12:02:56 2009 12, 28 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4LH2tCq004631 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2009 12:02:56 -0500 12, 28 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 12, 28 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 12, 28 -- with ESMTP id {0KK000H4C7CVA6-at-emroute1.ornl.gov} for 12, 28 -- microscopy-at-microscopy.com; Thu, 21 May 2009 13:02:55 -0400 (EDT) 12, 28 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 12, 28 -- (PMDF V6.4 #31561) id {0KK000H017CVPD-at-emroute1.ornl.gov} ; Thu, 12, 28 -- 21 May 2009 13:02:55 -0400 (EDT) 12, 28 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 12, 28 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 12, 28 -- with ESMTP id {0KK000FB37CVXY-at-emroute1.ornl.gov} ; Thu, 12, 28 -- 21 May 2009 13:02:55 -0400 (EDT) 12, 28 -- Date: Thu, 21 May 2009 13:02:55 -0400 12, 28 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 12, 28 -- Subject: Piezoresponse Live Forum - May 22 12, 28 -- To: microscopy-at-microscopy.com 12, 28 -- Cc: Andrei Kholkin {kholkin-at-ua.pt} , 12, 28 -- Alexei Gruverman {agruverman2-at-unlnotes.unl.edu} , 12, 28 -- "Dawn A. Bonnell" {bonnell-at-lrsm.upenn.edu} , 12, 28 -- "Robert W. Carpick" {carpick-at-seas.upenn.edu} 12, 28 -- Message-id: {4A15893F.5000808-at-ornl.gov} 12, 28 -- MIME-version: 1.0 12, 28 -- Content-type: text/plain; charset=windows-1252; format=flowed 12, 28 -- Content-transfer-encoding: 8BIT 12, 28 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) ==============================End of - Headers==============================
From jonaggert-at-hotmail.com Thu May 21 12:39:29 2009 Return-Path: {jonaggert-at-hotmail.com} Received: from google.com (aac176.neoplus.adsl.tpnet.pl [83.25.2.176]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4LHdQZT020420 for {microscopylistserverarchive-at-microscopy.com} ; Thu, 21 May 2009 12:39:28 -0500 Received: from [151.70.44.142] (HELO google.com) by lucky-gat.net; Thu, 21 May 2009 19:39:22 +0200 Message-ID: {00000006839EF36439486483} Reply-To: Kezia Cross {1389jessica.broman-at-gmail.com}
If you mean p-type and n-type wafers then coated with Al, I would think this would work. The wafers would have to be cleaned and stripped of any oxide before putting down the Al or it will charge.
gary g.
At 06:42 AM 5/21/2009, you wrote:
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Does anyone have Balzers or Bal-Tec equipment used for preparing cryo samples in the northeast region and would they would be willing to provide access to a colleague of mine from the University of Maine? (his email is below) Please respond to me directly. Thank you.
Lesley Bechtold
"Hi Lesley, thanks in advance for any assistance you can provide in trying to locate the instrument that I need to use. I need to rapidly freeze aqueous solutions containing polymers such that the water vitrifies. From what I see in the literature the widely accepted means of doing this is using liquid propane as the cryogen. The sample is sandwiched between two copper planchettes and either rapidly immersed into liquid propane via a spring loaded device, or sprayed with liquid nitrogen. The Balzers company, now Bal-tec used to, and may still, make these devices for Freeze Fracture etc studies. I am hoping to find one in the North East that I can use on a fee for service basis. If you know of anyone that has one Iíd sincerely appreciate the help. Kind regards, Dave"
There has been some interest in the procedures we use for getting rid of osmium precipitates by using 2-mercaptoethanol in our specimen processing procedures, so I have posted our actual processing worksheet in Excel format on our website. You can link to it at http://www.emc.missouri.edu/pandp.htm. Click on Microwave Processing Worksheet.
Below is the formula we use to make 0.1M Na Cacodylate buffer with 0.13M sucrose and 0.01M 2-Mercaptoethanol:
62.5 ml of 0.4M Na Cacodylate buffer 11.12 g sucrose 0.18 ml 2- Mercaptoethanol
Bring to 250ml with ultrapure water.
Obviously, you can change or eliminate the sucrose, as well as add anything else that's compatible with the 2-Me.
Hope this useful to a few people.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 11, 27 -- From TindallR-at-missouri.edu Fri May 22 09:12:15 2009 11, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4MECEKN010021 11, 27 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 09:12:15 -0500 11, 27 -- X-IronPort-Anti-Spam-Filtered: true 11, 27 -- X-IronPort-Anti-Spam-Result: ApoEABxPFkrRauUp/2dsb2JhbADBdgEJh3WIToJWgTUF 11, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 11, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 22 May 2009 09:12:10 -0500 11, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 27 -- Fri, 22 May 2009 09:12:09 -0500 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- Subject: Precipitates and 2-mercaptoethanol 11, 27 -- Date: Fri, 22 May 2009 09:11:24 -0500 11, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8108-at-UM-XMAIL08.um.umsystem.edu} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Precipitates and 2-mercaptoethanol 11, 27 -- Thread-Index: Acna5zCPbLMsGhL3SOye1OX31Z4pyg== 11, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 11, 27 -- To: {microscopy-at-microscopy.com} 11, 27 -- X-OriginalArrivalTime: 22 May 2009 14:12:09.0948 (UTC) FILETIME=[4BB8E9C0:01C9DAE7] 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4MECEKN010021 ==============================End of - Headers==============================
Dear List, What is the highest pressure ESEM? Does any ESEM have as much as one atmosphere? Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Fri May 22 12:20:59 2009 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4MHKwY0001677 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 12:20:59 -0500 2, 20 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by fire-doxen-postvirus (Postfix) with ESMTP id A3B562E50D7A 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 10:20:58 -0700 (PDT) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 2, 20 -- by fire-doxen-ssl (Postfix) with ESMTP id 91DEE2E5073D 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 22 May 2009 10:20:57 -0700 (PDT) 2, 20 -- Message-Id: {A64B037E-54B5-4B7E-97C1-E8CC1D83A9C1-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v935.3) 2, 20 -- Subject: ESEM 2, 20 -- Date: Fri, 22 May 2009 10:20:55 -0700 2, 20 -- X-Mailer: Apple Mail (2.935.3) ==============================End of - Headers==============================
I understood that FEI had the only true E-SEM since they took over Electro-Scan. I recall the maximum pressure was 10 torr. That was enough to maintain equilibrium with water vapor at room temperature. I seem to recall the maximum partial pressure of water was around 5 torr at 20 C. (I suppose I could look it up.)
What do you have in mind that you would need 1 atm?
For comparison, most VP-SEMs seem to max out at around 2 torr.
Warren S.
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Friday, May 22, 2009 12:22 PM To: wesaia-at-iastate.edu
Dear List, What is the highest pressure ESEM? Does any ESEM have as much as one atmosphere? Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 12, 36 -- From wesaia-at-iastate.edu Fri May 22 13:54:49 2009 12, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4MIsnJq020000 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:49 -0500 12, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 12, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4MIsmId020367 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500 12, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 12, 36 -- id 04a1_067fda08_4702_11de_b35f_001372578af6; 12, 36 -- Fri, 22 May 2009 13:54:48 -0500 12, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 12, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id n4MIsmuE011738 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500 12, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 12, 36 -- Fri, 22 May 2009 13:54:48 -0500 12, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 36 -- Content-class: urn:content-classes:message 12, 36 -- MIME-Version: 1.0 12, 36 -- Content-Type: text/plain; 12, 36 -- charset="us-ascii" 12, 36 -- Subject: RE: [Microscopy] ESEM 12, 36 -- Date: Fri, 22 May 2009 13:56:01 -0500 12, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE8091-at-maire.eng.iastate.edu} 12, 36 -- In-Reply-To: {200905221721.n4MHLnT4002721-at-ns.microscopy.com} 12, 36 -- X-MS-Has-Attach: 12, 36 -- X-MS-TNEF-Correlator: 12, 36 -- Thread-Topic: [Microscopy] ESEM 12, 36 -- Thread-Index: AcnbAcwnkMtbRInoSrCiXF4/KNWO3QADLH2w 12, 36 -- References: {200905221721.n4MHLnT4002721-at-ns.microscopy.com} 12, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 12, 36 -- To: {Microscopy-at-microscopy.com} 12, 36 -- X-OriginalArrivalTime: 22 May 2009 18:54:48.0676 (UTC) FILETIME=[C7E97240:01C9DB0E] 12, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.22.183735 12, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='FROM_SAME_AS_TO 0.05, BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FROM_SAME_AS_TO2 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0' 12, 36 -- Content-Transfer-Encoding: 8bit 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4MIsnJq020000 ==============================End of - Headers==============================
Today's count was 13 automated out-of-office replies to my post of earlier this afternoon. All but one of those was for people returning to the office next Tuesday (or Monday for those outside the USA).
I'm always a little curious how many of those messages I am going to get when I post. The number is up a little this time. Some of you must have started the Memorial Day holiday weekend a little early.
Let me take the opportunity to remind everyone that the list guidelines ask that people unsubscribe if they are going to engage an automated out-of-office reply. Your replies got to everyone who posts to the list as well as to your more personal correspondents.
Even though I have gotten to know some of you well enough that I might even count you as friends, I still don't really care to know when you are on vacation or traveling. {g}
To those of you who are in the office and in the US, may you enjoy the extended weekend.
Warren S.
==============================Original Headers============================== 7, 34 -- From wesaia-at-iastate.edu Fri May 22 16:00:51 2009 7, 34 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4ML0ogP006578 7, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 16:00:51 -0500 7, 34 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 7, 34 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4ML0nqt031824 7, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 16:00:49 -0500 7, 34 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-10.iastate.edu with smtp 7, 34 -- id 055b_a0f09ce2_4713_11de_801c_00137253420a; 7, 34 -- Fri, 22 May 2009 16:00:48 -0500 7, 34 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 7, 34 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n4ML0l1R009381 7, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 16:00:47 -0500 7, 34 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 34 -- Fri, 22 May 2009 16:00:48 -0500 7, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 34 -- Content-class: urn:content-classes:message 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; 7, 34 -- charset="us-ascii" 7, 34 -- Subject: Greeting to those of you in the office 7, 34 -- Date: Fri, 22 May 2009 16:02:01 -0500 7, 34 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703DE80C4-at-maire.eng.iastate.edu} 7, 34 -- X-MS-Has-Attach: 7, 34 -- X-MS-TNEF-Correlator: 7, 34 -- Thread-Topic: Greeting to those of you in the office 7, 34 -- Thread-Index: AcnbII059FwfaeB+S+Wp1d4c6kGlIA== 7, 34 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 7, 34 -- To: {Microscopy-at-microscopy.com} 7, 34 -- X-OriginalArrivalTime: 22 May 2009 21:00:48.0487 (UTC) FILETIME=[61E90F70:01C9DB20] 7, 34 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.22.204638 7, 34 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, BODY_SIZE_900_999 0, ECARD_GREETING_WORD 0, TO_NO_NAME 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0' 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4ML0ogP006578 ==============================End of - Headers==============================
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Question: Hi, As well as I know the max pressure in ESEM mode (FEI SEM) depends on the PLA - pressure limiting aperture, and could be 40 or 20 Torr. Sure, to realize the ESEM at such high pressure cooling of the sample is obligatory. Therefore small Peltier attachment to stage MUST present as a part of setup for ESEM experiment. Best, Inna
Zeiss, who bought out Cambridge, have a range of ESEMs - we purchased the EVO LS 15 last year. Much cheaper than FEI though not up to FEI performance, but will image material in up to a few Torr (had to look this up, work only in Pa these days...). Unfortunately, it won't image material cooled on the Peltier stage with water vapour in the chamber (as claimed) unless you go to higher vacuum - the low-vacuum detector is not up to scratch yet. For ESEM at lowest vacuum you'd need one of the FEI Quanta range, but I don't imagine you'd be able to see anything much at 1 atmosphere, the fog would be too thick. cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 23/05/09 5:00 AM, "wesaia-at-iastate.edu" {wesaia-at-iastate.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I understood that FEI had the only true E-SEM since they took over } Electro-Scan. I recall the maximum pressure was 10 torr. That was enough } to maintain equilibrium with water vapor at room temperature. I seem to } recall the maximum partial pressure of water was around 5 torr at 20 C. } (I suppose I could look it up.) } } What do you have in mind that you would need 1 atm? } } For comparison, most VP-SEMs seem to max out at around 2 torr. } } Warren S. } } -----Original Message----- } X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] } Sent: Friday, May 22, 2009 12:22 PM } To: wesaia-at-iastate.edu } Subject: [Microscopy] ESEM } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Dear List, } What is the highest pressure ESEM? Does any ESEM have as much } as one } atmosphere? } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } } } ==============================Original Headers============================== } 12, 36 -- From wesaia-at-iastate.edu Fri May 22 13:54:49 2009 } 12, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu } [129.186.140.15]) } 12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n4MIsnJq020000 } 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:49 -0500 } 12, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu } [129.186.1.48]) } 12, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id } n4MIsmId020367 } 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500 } 12, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by } devirus-11.iastate.edu with smtp } 12, 36 -- id 04a1_067fda08_4702_11de_b35f_001372578af6; } 12, 36 -- Fri, 22 May 2009 13:54:48 -0500 } 12, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu } [129.186.23.85]) } 12, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id } n4MIsmuE011738 } 12, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2009 13:54:48 -0500 } 12, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by } owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); } 12, 36 -- Fri, 22 May 2009 13:54:48 -0500 } 12, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 12, 36 -- Content-class: urn:content-classes:message } 12, 36 -- MIME-Version: 1.0 } 12, 36 -- Content-Type: text/plain; } 12, 36 -- charset="us-ascii" } 12, 36 -- Subject: RE: [Microscopy] ESEM } 12, 36 -- Date: Fri, 22 May 2009 13:56:01 -0500 } 12, 36 -- Message-ID: } {16A330AC32056A40B32842EC4BB8D72703DE8091-at-maire.eng.iastate.edu} } 12, 36 -- In-Reply-To: {200905221721.n4MHLnT4002721-at-ns.microscopy.com} } 12, 36 -- X-MS-Has-Attach: } 12, 36 -- X-MS-TNEF-Correlator: } 12, 36 -- Thread-Topic: [Microscopy] ESEM } 12, 36 -- Thread-Index: AcnbAcwnkMtbRInoSrCiXF4/KNWO3QADLH2w } 12, 36 -- References: {200905221721.n4MHLnT4002721-at-ns.microscopy.com} } 12, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} } 12, 36 -- To: {Microscopy-at-microscopy.com} } 12, 36 -- X-OriginalArrivalTime: 22 May 2009 18:54:48.0676 (UTC) } FILETIME=[C7E97240:01C9DB0E] } 12, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, } Antispam-Data: 2009.5.22.183735 } 12, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, } Report='FROM_SAME_AS_TO 0.05, BODY_SIZE_1300_1399 0, BODY_SIZE_2000_LESS 0, } BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, TO_NO_NAME 0, } __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __C230066_P5 0, __CP_URI_IN_BODY } 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FROM_SAME_AS_TO2 0, __HAS_MSGID 0, } __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, } __SANE_MSGID 0, __TO_MALFORMED_2 0' } 12, 36 -- Content-Transfer-Encoding: 8bit } 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4MIsnJq020000 } ==============================End of - Headers==============================
Independently of the main message of your mail, I think it is a mistake to calculate in 3D even though we are talking about gas here. This is because N2 will not fill the room, but will fall on the floor. So I would be more realistic to calculate how high the level of N2 can be considering the surface of the actual room you are using. Then take into account the possibility that someone places his face near the ground for any reason, just to pick up (or search for) a pen which has fallen to the ground for example.
Regards, Stephane
----- Original Message ---- X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu} To: nizets2-at-yahoo.com Sent: Wednesday, May 20, 2009 3:04:22 PM
John:
Are you seriously stirring this up again? Didn't we just handle O2 displacement via nitrogen boil off a few weeks ago?
The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a tiny room for a scope and all the N2 would have to be released rapidly enough to displace the O2 in the room (or reduce it from 22% to below 12% for this to become an issue let alone life threatening) without the N2 leaking out the doorway or any O2 leaking in. But in fact it sounds like ". . .going through nitrogen gas like there is no tomorrow." is probably days not minutes, and the scope room is a "normal room" and not a sealed environmental chamber.
I have worked with compressed atmospheric air. No matter how dry it is an extremely corrosive material, degrading fittings, seals and regulator parts. I would suggest the long term costs and safety make working with compressed air a far worse choice than ppN2
On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Elgin; } My I suggest also that you have created a significant safety hazard by } running your air table, and I presume your valving, on nitrogen. The } leak you now have (and everything develops leaks eventually) is } filling up your room with pure nitrogen and displacing the oxygen. You } should be using only clean dry air for these items so that when it } leaks, the room fills up with.....air! Air that you can breath! } Nitrogen should only be used for venting the chamber. } } } Sent from my iPhone. } John Mardinly } } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote: } } } } } } } } } --- } } --- } } -------------------------------------------------------------------- } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Wed May 20 07:53:35 2009 10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4KCrZNA004846 10, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 20 May 2009 07:53:35 -0500 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrsGt014924; 10, 25 -- Wed, 20 May 2009 08:53:54 -0400 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id n4KCrYF2021966; 10, 25 -- Wed, 20 May 2009 08:53:34 -0400 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: microscopy-at-Microscopy.com 10, 25 -- Date: Wed, 20 May 2009 08:53:34 -0400 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak 10, 25 -- CC: "jmardinly-at-gmail.com" {jmardinly-at-gmail.com} 10, 25 -- Message-ID: {4A13C50E.27113.A79CE8A-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200905082112.n48LCJEb013069-at-ns.microscopy.com} 10, 25 -- References: {200905082112.n48LCJEb013069-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 25 -- From nizets2-at-yahoo.com Tue May 26 04:54:37 2009 26, 25 -- Received: from web110804.mail.gq1.yahoo.com (web110804.mail.gq1.yahoo.com [67.195.13.227]) 26, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4Q9sa0L026752 26, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 04:54:36 -0500 26, 25 -- Received: (qmail 78109 invoked by uid 60001); 26 May 2009 09:54:35 -0000 26, 25 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243331675; bh=FPihZg+VPIBsWzBKW7S9flOM9f7OL/wjPlgVw1cGIKQ=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=UM7N13p3BBmsfnFFw6qvwHkvCqRYdPOEawnl2xMAba5rZO0zLFQU6d2DXPo6ikOJLC6lxnHlzOuBUZPiWCYF01kAZggA9W2XEsDcf9sLAu20Mkayo6Dg/rft3fNKunfrKKUTjEbtJlOyqzZ3Tt2rvUvmr7y6moUb5DAtOULH1DQ= 26, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 26, 25 -- s=s1024; d=yahoo.com; 26, 25 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 26, 25 -- b=OnpWwjWCf07a3DDVUnu6CeTOkGa+kG9Rsgy2J/SWbm+mb/yRreYvdFXdajZ0VHioG5LxhDmzgJaH6/6qMwXIPxXu4/oFVXg1d8+g9Mn127Ul29Oe639nSe/bc+oS/FA55ALnK6gJFdOS/v7ccYosQRJZxzfasq+hmaatbGeKvnk=; 26, 25 -- Message-ID: {649345.78052.qm-at-web110804.mail.gq1.yahoo.com} 26, 25 -- X-YMail-OSG: JAeLrdEVM1lQq.nCnfKIL2Iol4eVVudxaAYM8H2AkrjFb5iHjCBxvGFbxmID.MIr0BLUKWjHzFJudvLiTeaDuBf7m4QqMK6nAKPxGz6zl78Jd2oDIpJzJ0YsSyUeyb1ACOYruODGsF5i1VviXD.oT6vJATUdBlzdOQ.BAJ87JnWvJ.EKDLgrZNNTNNWgXvKHV04TJBAZoAUX5cw_b8Q_c3Xt7x8bIooh_vt7zggHnfUC6VGzE5Z_wR_5xA9iGXTXDb6D4JYh3zs.w0reKTHZeLIevMwe9Pp1w1Htor_oQaw1dw9eX9z2MwpNA1rRLTXfkUcoysnTkyDWS.m3JA-- 26, 25 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Tue, 26 May 2009 02:54:35 PDT 26, 25 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10 26, 25 -- References: {200905201304.n4KD4MU5011688-at-ns.microscopy.com} 26, 25 -- Date: Tue, 26 May 2009 02:54:35 -0700 (PDT) 26, 25 -- From: Stephane Nizet {nizets2-at-yahoo.com} 26, 25 -- Subject: Re: [Microscopy] viaWWW: Nitrogen leak 26, 25 -- To: edelmare-at-muohio.edu 26, 25 -- Cc: microscopy-at-microscopy.com 26, 25 -- In-Reply-To: {200905201304.n4KD4MU5011688-at-ns.microscopy.com} 26, 25 -- MIME-Version: 1.0 26, 25 -- Content-Type: text/plain; charset=iso-8859-1 26, 25 -- Content-Transfer-Encoding: 8bit 26, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4Q9sa0L026752 ==============================End of - Headers==============================
This subject is partly relevant to electron microscopy since TEM and SEM are parts of the analysis procedure. I am interested in the redox properties of some minerals. Some minerals (namely layered silicates but I also read something about amphiboles) seem to be able to reduce metals in solutions, leading to their precipitation. The precipitation process does not occur uniformly at the surface of the minerals but is dependent on the structure. A paper of particular interest is "Reaction of some trioctahedral micas with copper sulfate solutions at 25°C and 1 atm; an electron probe and TEM investigation" by Ilton et al. (1992).
Unfortunately, being a biologist I have no access to papers in earth science (which demonstrates the sad partitioning in science), not even the paper I cited above.
I would like to know the procedure used by these authors (or others). I also read about the possibility to precipitate silver ions on biotite but I need more details.
So I send this message in the bottle to the list. This is a call to anybody in the field who could help me devise a protocol, or simply send me a copy of the above paper. My knowledge in mineralogy is limited (I have some background), so I would appreciate being able to discuss specific issues like which kind a mineral would be expected to have which redox property...
Best regards,
Stephane-without-a-i
==============================Original Headers============================== 11, 22 -- From nizets2-at-yahoo.com Tue May 26 09:07:40 2009 11, 22 -- Received: from web110806.mail.gq1.yahoo.com (web110806.mail.gq1.yahoo.com [67.195.13.229]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4QE7dUD024382 11, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:07:39 -0500 11, 22 -- Received: (qmail 49096 invoked by uid 60001); 26 May 2009 14:07:38 -0000 11, 22 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243346857; bh=UlO355lMNinYx75gKjClgVcwWXrtZY/bkCiR8ploQeI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=jRjj0vTZ+6RwYXxpYFweMBoXZgKGmtG5Hkq57FmsXUw38N0gHLBU7xpWo9pO1afJixXQSI2qWatEQhSJDi49mztFVnAO8FXcefZCviLS2On/oLcksUVrAptXKohJhVf0683UY2Kkxe2JCf52/BVytPTiQCiqkI+FNvHb7/RSNS8= 11, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 22 -- s=s1024; d=yahoo.com; 11, 22 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 11, 22 -- b=EfO94aDLWTRWAcrgkuYhA4UGGq46blHjxPR/8zukczi3SqE4QvO49dXpdwhcU3soKzRViPTo304kmxfkNiDtVeB8rUz/wgYrOuuQtGJ2erWhf60cGc4XAVfrl2hlFf+KaUHnsQRn54/M+2FbICo78+pqxWnPePPHIjV3/LP9faY=; 11, 22 -- Message-ID: {935923.48714.qm-at-web110806.mail.gq1.yahoo.com} 11, 22 -- X-YMail-OSG: CiWDi6UVM1mEEbRCcnB3FZ1_nN8UrSwB7HDmYb0QyLC206tlL_IH9C5MEd0lDc04MVmDdjoas49TAbJftX9.mFg104ecaEqIzr7Ru2xujiL0S6t.nZO3LPfA9L6XDWKhsyNfUaDDtaHIZQmP4srGj_hbunzZ.gNFNSkjfurh6lG5R9CizZfXwU.Yqbt4yz36UqaYMwtaGuvCX4N7e9Fgp6lz5_mcye1IiTpuQmD9QEihY5kn2ml5UYnjEmdKDEwnFqqlQRWwVu_NN.hr6GVDN57IISGKE70z03GCi_7BnI1kpeh8yIj4Mw-- 11, 22 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Tue, 26 May 2009 07:07:37 PDT 11, 22 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10 11, 22 -- Date: Tue, 26 May 2009 07:07:37 -0700 (PDT) 11, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 11, 22 -- Subject: The Redox properties of minerals 11, 22 -- To: microscopy-at-microscopy.com 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-Type: text/plain; charset=iso-8859-1 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QE7dUD024382 ==============================End of - Headers==============================
Are you sure the nitrogen will accumulate near the floor? This issue was about compressed nitrogen gas rather than LN2. Has anyone shown that this is an issue that we should be concerned about?
The N2 would cool a little upon expansion, but the flow would be slow enough and the cooling is not so drastic that I would not think a layer of cool gas would build up on the floor. Once warmed to room temperature, the N2 would be ever so slightly lighter than air. It seems this _should_ be treated as a 3-D dilution problem.
Warren
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, May 26, 2009 4:56 AM To: wesaia-at-iastate.edu
Hi!
Independently of the main message of your mail, I think it is a mistake to calculate in 3D even though we are talking about gas here. This is because N2 will not fill the room, but will fall on the floor. So I would be more realistic to calculate how high the level of N2 can be considering the surface of the actual room you are using. Then take into account the possibility that someone places his face near the ground for any reason, just to pick up (or search for) a pen which has fallen to the ground for example.
Regards, Stephane
----- Original Message ---- X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu} To: nizets2-at-yahoo.com Sent: Wednesday, May 20, 2009 3:04:22 PM
John:
Are you seriously stirring this up again? Didn't we just handle O2 displacement via nitrogen boil off a few weeks ago?
The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a tiny room for a scope and all the N2 would have to be released rapidly enough to displace the O2 in the room (or reduce it from 22% to below 12% for this to become an issue let alone life threatening) without the N2 leaking out the doorway or any O2 leaking in. But in fact it sounds like ". . .going through nitrogen gas like there is no tomorrow." is probably days not minutes, and the scope room is a "normal room" and not a sealed environmental chamber.
I have worked with compressed atmospheric air. No matter how dry it is an extremely corrosive material, degrading fittings, seals and regulator parts. I would suggest the long term costs and safety make working with compressed air a far worse choice than ppN2
On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Elgin; } My I suggest also that you have created a significant safety hazard by } running your air table, and I presume your valving, on nitrogen. The } leak you now have (and everything develops leaks eventually) is } filling up your room with pure nitrogen and displacing the oxygen. You } should be using only clean dry air for these items so that when it } leaks, the room fills up with.....air! Air that you can breath! } Nitrogen should only be used for venting the chamber. } } } Sent from my iPhone. } John Mardinly } } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote: } } } } } } } } } --- } } --- } } -------------------------------------------------------------------- } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help
==============================Original Headers============================== 20, 36 -- From wesaia-at-iastate.edu Tue May 26 09:20:57 2009 20, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 20, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QEKsRQ002148 20, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:20:55 -0500 20, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 20, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4QEKrpT006037; 20, 36 -- Tue, 26 May 2009 09:20:53 -0500 20, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp 20, 36 -- id 0c88_6bdcb654_4a00_11de_a285_001372578af6; 20, 36 -- Tue, 26 May 2009 09:20:53 -0500 20, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 20, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n4QEKqN3016931; 20, 36 -- Tue, 26 May 2009 09:20:52 -0500 20, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 20, 36 -- Tue, 26 May 2009 09:20:52 -0500 20, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 36 -- Content-class: urn:content-classes:message 20, 36 -- MIME-Version: 1.0 20, 36 -- Content-Type: text/plain; 20, 36 -- charset="iso-8859-1" 20, 36 -- Subject: RE: [Microscopy] Re: viaWWW: Nitrogen leak 20, 36 -- Date: Tue, 26 May 2009 09:22:07 -0500 20, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703E4A06B-at-maire.eng.iastate.edu} 20, 36 -- In-Reply-To: {200905260956.n4Q9u554028058-at-ns.microscopy.com} 20, 36 -- X-MS-Has-Attach: 20, 36 -- X-MS-TNEF-Correlator: 20, 36 -- Thread-Topic: [Microscopy] Re: viaWWW: Nitrogen leak 20, 36 -- Thread-Index: Acnd6DD2Rgqp93I5SXyQuSYC2FQUAQAI46fg 20, 36 -- References: {200905260956.n4Q9u554028058-at-ns.microscopy.com} 20, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 20, 36 -- To: {nizets2-at-yahoo.com} , {Microscopy-at-microscopy.com} 20, 36 -- X-OriginalArrivalTime: 26 May 2009 14:20:52.0231 (UTC) FILETIME=[2CADF970:01C9DE0D] 20, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.26.141105 20, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0' 20, 36 -- Content-Transfer-Encoding: 8bit 20, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QEKsRQ002148 ==============================End of - Headers==============================
Stumm, W. : Chemistry of the solid-water interface: Processes at the mineral-water and particle-water interface in natural systems. John Wiley Sons, 605 Third Ave., New York, NY 10158-0012 (United States). 1992
3 And probably the best thing:
Do a search on Metal Oxide - Mica Pigments as these as newer Luster pigments created by coating metal oxides onto mica (muscovite/biotite).
Tony
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-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, May 26, 2009 10:18 AM To: ph2-at-sprynet.com
Hi all!
This subject is partly relevant to electron microscopy since TEM and SEM are parts of the analysis procedure. I am interested in the redox properties of some minerals. Some minerals (namely layered silicates but I also read something about amphiboles) seem to be able to reduce metals in solutions, leading to their precipitation. The precipitation process does not occur uniformly at the surface of the minerals but is dependent on the structure. A paper of particular interest is "Reaction of some trioctahedral micas with copper sulfate solutions at 25°C and 1 atm; an electron probe and TEM investigation" by Ilton et al. (1992).
Unfortunately, being a biologist I have no access to papers in earth science (which demonstrates the sad partitioning in science), not even the paper I cited above.
I would like to know the procedure used by these authors (or others). I also read about the possibility to precipitate silver ions on biotite but I need more details.
So I send this message in the bottle to the list. This is a call to anybody in the field who could help me devise a protocol, or simply send me a copy of the above paper. My knowledge in mineralogy is limited (I have some background), so I would appreciate being able to discuss specific issues like which kind a mineral would be expected to have which redox property...
Best regards,
Stephane-without-a-i
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==============================Original Headers============================== 29, 29 -- From ph2-at-sprynet.com Tue May 26 09:45:50 2009 29, 29 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 29, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QEjmYx021375 29, 29 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:45:49 -0500 29, 29 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 29, 29 -- s=dk20050327; d=sprynet.com; 29, 29 -- b=YmDlvrDd8Mbi1RO0nj87At7seDRMhO6+EFTHHjGL3CKB9I4b9Jds1XSJYIttJtFj; 29, 29 -- h=Received:From:To:Cc:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 29, 29 -- Received: from [75.61.18.94] (helo=user915fa8f284) 29, 29 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 29, 29 -- (envelope-from {ph2-at-sprynet.com} ) 29, 29 -- id 1M8xuS-0007Ns-FH; Tue, 26 May 2009 10:45:44 -0400 29, 29 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 29, 29 -- To: {nizets2-at-yahoo.com} 29, 29 -- Cc: "Microscopy Listserve" {microscopy-at-microscopy.com} 29, 29 -- Subject: RE: [Microscopy] The Redox properties of minerals 29, 29 -- Date: Tue, 26 May 2009 10:45:39 -0400 29, 29 -- MIME-Version: 1.0 29, 29 -- Content-Type: text/plain; 29, 29 -- charset="iso-8859-1" 29, 29 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 29, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 29, 29 -- Thread-Index: AcneDMTU0fXg8Vp9RMioRwZrmJ1fegAAvx1g 29, 29 -- In-Reply-To: {200905261417.n4QEHt3O031880-at-ns.microscopy.com} 29, 29 -- Message-ID: {E1M8xuS-0007Ns-FH-at-elasmtp-dupuy.atl.sa.earthlink.net} 29, 29 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f94068f1d8be47278b1331ba7cc94569b6350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 29, 29 -- X-Originating-IP: 75.61.18.94 29, 29 -- Content-Transfer-Encoding: 8bit 29, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QEjmYx021375 ==============================End of - Headers==============================
I haven't followed this whole thread, but I keep seeing messages pop up on this topic. Maybe I missed something but is this really an issue? A "standard" "200" cylinder of N2 (70 lbs, 2490psi) is listed as containing 255cu ft (at STP, I assume, I didn't do the math...) and this is only ~1/3 the volume of a 10ftx10ft room (8 ft ceilings, neglecting installed content volume) even if it were instantly allowed to fill the room; yes this would make the reduced O2 availability equivalent to the "death zone" of mountain climbing (http://en.wikipedia.org/wiki/Death_zone), although in that case O2 availability is reduced due to reduced pressure, not simply concentration.... But death zone death is not instantaneous and you would probably get loopy and sick but have enough wits to get to fresh air. Most people would be well aware of a leak of that magnitude (would sound like a deafening explosive "whoosh" that would interrupt even my deepest nap). Rooms should have some ventillation that will draw in fresh air. If I am going through a tank a month, I consider this a leak that needs attention - because of trouble and expense more than other reasons.
Most microscope rooms are probably something like 10'x10' for practical reasons. If we only connect one tank at a time, it limits the risk. Or else, use of a smaller tank will reduce the risk further. Make sure you have code-compliant ventilation - a good idea anyway*.
So it this really a problem worth this level of concern? Has anyone ever had a problem or been harmed?
Dale
*Ventilation can be an issue with TEM specs. A microscope I am familiar with specs an air flow limitation that precludes breathing by the operator. I haven't seen data for other manufacturers.
wesaia-at-iastate.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Are you sure the nitrogen will accumulate near the floor? This issue was about compressed nitrogen gas rather than LN2. Has anyone shown that this is an issue that we should be concerned about? } } The N2 would cool a little upon expansion, but the flow would be slow enough and the cooling is not so drastic that I would not think a layer of cool gas would build up on the floor. Once warmed to room temperature, the N2 would be ever so slightly lighter than air. It seems this _should_ be treated as a 3-D dilution problem. } } Warren } } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Tuesday, May 26, 2009 4:56 AM } To: wesaia-at-iastate.edu } Subject: [Microscopy] Re: viaWWW: Nitrogen leak } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi! } } Independently of the main message of your mail, I think it is a mistake to calculate in 3D even though we are talking about gas here. } This is because N2 will not fill the room, but will fall on the floor. So I would be more realistic to calculate how high the level of N2 can be considering the surface of the actual room you are using. Then take into account the possibility that someone places his face near the ground for any reason, just to pick up (or search for) a pen which has fallen to the ground for example. } } Regards, } Stephane } } ----- Original Message ---- } X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu} } To: nizets2-at-yahoo.com } Sent: Wednesday, May 20, 2009 3:04:22 PM } Subject: [Microscopy] viaWWW: Nitrogen leak } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } John: } } Are you seriously stirring this up again? Didn't we just handle O2 } displacement via nitrogen boil off a few weeks ago? } } The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains only } 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at } 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a } tiny room for a scope and all the N2 would have to be released } rapidly enough to displace the O2 in the room (or reduce it from 22% } to below 12% for this to become an issue let alone life threatening) } without the N2 leaking out the doorway or any O2 leaking in. But in } fact it sounds like ". . .going through nitrogen gas like there is no } tomorrow." is probably days not minutes, and the scope room is a } "normal room" and not a sealed environmental chamber. } } I have worked with compressed atmospheric air. No matter how dry it } is an extremely corrosive material, degrading fittings, seals and } regulator parts. I would suggest the long term costs and safety make } working with compressed air a far worse choice than ppN2 } } } On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote: } } } } } } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Elgin; } } My I suggest also that you have created a significant safety hazard by } } running your air table, and I presume your valving, on nitrogen. The } } leak you now have (and everything develops leaks eventually) is } } filling up your room with pure nitrogen and displacing the oxygen. You } } should be using only clean dry air for these items so that when it } } leaks, the room fills up with.....air! Air that you can breath! } } Nitrogen should only be used for venting the chamber. } } } } } } Sent from my iPhone. } } John Mardinly } } } } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote: } } } } } } } } } } } --- } } } --- } } } -------------------------------------------------------------------- } } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } of America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html --- --- } } } -------------------------------------------------------------------- } } } -- } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at } } } http://www.microscopy.com/MLFormMail.html --- --- } } } -------------------------------------------------------------------- } } } - Remember this posting is most likely not from a Subscriber, so } } } when replying please copy both eawoodruff-at-live.com as well as } } } the MIcroscopy Listserver --- --- } } } -------------------------------------------------------------------- } } } - } } } } } } Email: eawoodruff-at-live.com } } } Name: Elvin Woodruff III } } } } } } Organization: Tennessee State University } } } } } } Title-Subject: [Filtered] Nitrogen leak } } } } } } Question: Hi All, } } } } } } I have recently started working with a JEOL 6701F scanning electron } } } microscopy. The scope is running just fine, but it is going through } } } nitrogen gas like there is not tomorrow. The scope sits on an air } } } table which is supplied by gas (could this be it?)and other areas } } } such as the specimen load chamber is also filled with nitrogen gas. } } } I have talked to a couple of other people about the gas loss but to } } } no avail. I have checked all of the usual places for a leak but } } } can't find anything. We don't have a service contract right now so } } } any help or ideas on why we are going through so much gas would be a } } } big help. } } } } } } Thanks } } } } } } Elvin } } } } } } Login Host: 68.19.196.245 } } } -------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 20, 36 -- From wesaia-at-iastate.edu Tue May 26 09:20:57 2009 } 20, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) } 20, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QEKsRQ002148 } 20, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 09:20:55 -0500 } 20, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) } 20, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id n4QEKrpT006037; } 20, 36 -- Tue, 26 May 2009 09:20:53 -0500 } 20, 36 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-11.iastate.edu with smtp } 20, 36 -- id 0c88_6bdcb654_4a00_11de_a285_001372578af6; } 20, 36 -- Tue, 26 May 2009 09:20:53 -0500 } 20, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) } 20, 36 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id n4QEKqN3016931; } 20, 36 -- Tue, 26 May 2009 09:20:52 -0500 } 20, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); } 20, 36 -- Tue, 26 May 2009 09:20:52 -0500 } 20, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 20, 36 -- Content-class: urn:content-classes:message } 20, 36 -- MIME-Version: 1.0 } 20, 36 -- Content-Type: text/plain; } 20, 36 -- charset="iso-8859-1" } 20, 36 -- Subject: RE: [Microscopy] Re: viaWWW: Nitrogen leak } 20, 36 -- Date: Tue, 26 May 2009 09:22:07 -0500 } 20, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72703E4A06B-at-maire.eng.iastate.edu} } 20, 36 -- In-Reply-To: {200905260956.n4Q9u554028058-at-ns.microscopy.com} } 20, 36 -- X-MS-Has-Attach: } 20, 36 -- X-MS-TNEF-Correlator: } 20, 36 -- Thread-Topic: [Microscopy] Re: viaWWW: Nitrogen leak } 20, 36 -- Thread-Index: Acnd6DD2Rgqp93I5SXyQuSYC2FQUAQAI46fg } 20, 36 -- References: {200905260956.n4Q9u554028058-at-ns.microscopy.com} } 20, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} } 20, 36 -- To: {nizets2-at-yahoo.com} , {Microscopy-at-microscopy.com} } 20, 36 -- X-OriginalArrivalTime: 26 May 2009 14:20:52.0231 (UTC) FILETIME=[2CADF970:01C9DE0D] } 20, 36 -- X-PMX-Version: 5.5.3.366731, Antispam-Engine: 2.7.0.366912, Antispam-Data: 2009.5.26.141105 } 20, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='SUPERLONG_LINE 0.05, BODY_SIZE_6000_6999 0, BODY_SIZE_7000_LESS 0, ECARD_KNOWN_DOMAINS 0, TO_NO_NAME 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_XOAT 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __TO_MALFORMED_2 0' } 20, 36 -- Content-Transfer-Encoding: 8bit } 20, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QEKsRQ002148 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 22 -- From dac-at-research.umass.edu Tue May 26 10:16:39 2009 11, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QFGdgc004220 11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 10:16:39 -0500 11, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 11, 22 -- (authenticated bits=0) 11, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id n4QFGcKg015172 11, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 11:16:39 -0400 11, 22 -- Message-ID: {4A1C07D6.3040802-at-research.umass.edu} 11, 22 -- Date: Tue, 26 May 2009 11:16:38 -0400 11, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 11, 22 -- Reply-To: dac-at-research.umass.edu 11, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.8.1.21) Gecko/20090403 SeaMonkey/1.1.16 11, 22 -- MIME-Version: 1.0 11, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 11, 22 -- Subject: Re: [Microscopy] viaWWW: Nitrogen leak (is this really a problem?) 11, 22 -- References: {200905261426.n4QEQqUw014299-at-ns.microscopy.com} 11, 22 -- In-Reply-To: {200905261426.n4QEQqUw014299-at-ns.microscopy.com} 11, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 22 -- Content-Transfer-Encoding: 7bit 11, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Nitrogen gas is lighter than the air and so will rather go up than down. What tricks us into assuming the opposite is the condensed water "steam" that appears when LN2 is being poured.
Now, I hope this was not a bait to see who's a smart^$$ ;)
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
Begin forwarded message:
} From: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } Date: May 26, 2009 5:56:17 AM EDT } To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com} } Subject: [Microscopy] Re: viaWWW: Nitrogen leak } Reply-To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi! } } Independently of the main message of your mail, I think it is a } mistake to calculate in 3D even though we are talking about gas here. } This is because N2 will not fill the room, but will fall on the } floor. So I would be more realistic to calculate how high the level } of N2 can be considering the surface of the actual room you are } using. Then take into account the possibility that someone places } his face near the ground for any reason, just to pick up (or search } for) a pen which has fallen to the ground for example. } } Regards, } Stephane } } } } ----- Original Message ---- } X-from: "edelmare-at-muohio.edu" {edelmare-at-muohio.edu} } To: nizets2-at-yahoo.com } Sent: Wednesday, May 20, 2009 3:04:22 PM } Subject: [Microscopy] viaWWW: Nitrogen leak } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } John: } } Are you seriously stirring this up again? Didn't we just handle O2 } displacement via nitrogen boil off a few weeks ago? } } The standard CGA ppN2 (Pre-Purified Nitrogen) gas tank contains } only } 300 cubic feet of nitrogen. That's a space 6.7' x 6.7' x 6.7' at } 100% N2 (or better yet a space 2 m x 2 m x 2 m) - this would be a } tiny room for a scope and all the N2 would have to be released } rapidly enough to displace the O2 in the room (or reduce it from 22% } to below 12% for this to become an issue let alone life threatening) } without the N2 leaking out the doorway or any O2 leaking in. But in } fact it sounds like ". . .going through nitrogen gas like there is no } tomorrow." is probably days not minutes, and the scope room is a } "normal room" and not a sealed environmental chamber. } } I have worked with compressed atmospheric air. No matter how dry } it } is an extremely corrosive material, degrading fittings, seals and } regulator parts. I would suggest the long term costs and safety make } working with compressed air a far worse choice than ppN2 } } } On 8 May 2009 at 17:12, jmardinly-at-gmail.com wrote: } } } } } } } } } ---------------------------------------------------------------------- } } ------ The Microscopy ListServer -- CoSponsor: The Microscopy } } Society } } of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Elgin; } } My I suggest also that you have created a significant safety hazard } } by } } running your air table, and I presume your valving, on nitrogen. The } } leak you now have (and everything develops leaks eventually) is } } filling up your room with pure nitrogen and displacing the oxygen. } } You } } should be using only clean dry air for these items so that when it } } leaks, the room fills up with.....air! Air that you can breath! } } Nitrogen should only be used for venting the chamber. } } } } } } Sent from my iPhone. } } John Mardinly } } } } On May 8, 2009, at 5:52 AM, eawoodruff-at-live.com wrote: } } } } } } } } } } } } } } --- } } } --- } } } -------------------------------------------------------------------- } } } -- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } of America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html --- --- } } } -------------------------------------------------------------------- } } } -- } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at } } } http://www.microscopy.com/MLFormMail.html --- --- } } } -------------------------------------------------------------------- } } } - Remember this posting is most likely not from a Subscriber, so } } } when replying please copy both eawoodruff-at-live.com as well as } } } the MIcroscopy Listserver --- --- } } } -------------------------------------------------------------------- } } } - } } } } } } Email: eawoodruff-at-live.com } } } Name: Elvin Woodruff III } } } } } } Organization: Tennessee State University } } } } } } Title-Subject: [Filtered] Nitrogen leak } } } } } } Question: Hi All, } } } } } } I have recently started working with a JEOL 6701F scanning electron } } } microscopy. The scope is running just fine, but it is going through } } } nitrogen gas like there is not tomorrow. The scope sits on an air } } } table which is supplied by gas (could this be it?)and other areas } } } such as the specimen load chamber is also filled with nitrogen gas. } } } I have talked to a couple of other people about the gas loss but to } } } no avail. I have checked all of the usual places for a leak but } } } can't find anything. We don't have a service contract right now so } } } any help or ideas on why we are going through so much gas would be a } } } big help. } } } } } } Thanks } } } } } } Elvin } } } } } } Login Host: 68.19.196.245 } } } --- } } } --- } } } -------------------------------------------------------------------- } } } - } } } } } } ==============================Original } } } Headers============================== } } } 9, 11 -- From zaluzec-at-microscopy.com Fri May 8 07:41:46 2009 } } } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } } [206.69.208.22 } } } ]) } } } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id n48CfjvE003327 9, 11 -- for {microscopy-at-microscopy.com} ; } } } Fri, 8 May 2009 07:41:46 -0500 9, 11 -- Mime-Version: 1.0 9, 11 -- } } } Message-Id: {p06240801c629d8f35379-at-[206.69.208.22]} 9, 11 -- Date: } } } Fri, 8 May 2009 07:41:44 -0500 9, 11 -- To: } } } microscopy-at-microscopy.com 9, 11 -- From: eawoodruff-at-live.com (by way } } } of MicroscopyListserver) 9, 11 -- Subject: viaWWW: Nitrogen leak 9, } } } 11 -- Content-Type: text/plain; charset="us-ascii" ; } } } format="flowed" ==============================End of - } } } Headers============================== } } } } ==============================Original } } Headers============================== 5, 43 -- From } } jmardinly-at-gmail.com Fri May 8 16:10:01 2009 5, 43 -- Received: from } } wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.180]) 5, } } 43 } } -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } n48L9w3w012003 5, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 8 } } May } } 2009 16:10:00 -0500 5, 43 -- Received: by wa-out-1112.google.com with
Okay, here's a weird one. Has anyone heard of, or better yet, had any experience with, using ortho-phthalaldehyde as a substitute for glutaraldehyde in EM fixation? Cydex-OPA, in particular?
Seems one of our local hospitals is kicking about their researchers fixing tissues for EM in glut-based fixatives and is pushing them hard to find a substitute. My searches turn this up as a disinfectant, but our director says it theoretically might work, based on the fact that it's a dialdehyde.
Could this be the next great breakthrough in TEM?
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com
==============================Original Headers============================== 9, 27 -- From TindallR-at-missouri.edu Tue May 26 13:49:36 2009 9, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QInaYQ011348 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:49:36 -0500 9, 27 -- X-IronPort-Anti-Spam-Filtered: true 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPPWG0rRauUp/2dsb2JhbAC/AQEJhmCIToJWgTUF 9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 9, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 26 May 2009 13:49:36 -0500 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 27 -- Tue, 26 May 2009 13:49:36 -0500 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="us-ascii" 9, 27 -- Subject: TEM: ortho-phthalaldehyde 9, 27 -- Date: Tue, 26 May 2009 13:49:35 -0500 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8113-at-UM-XMAIL08.um.umsystem.edu} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: TEM: ortho-phthalaldehyde 9, 27 -- Thread-Index: AcneMrcC1u+u63FzQ4q+V006CgKFUw== 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 27 -- To: {microscopy-at-microscopy.com} 9, 27 -- X-OriginalArrivalTime: 26 May 2009 18:49:36.0075 (UTC) FILETIME=[B73D51B0:01C9DE32] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4QInaYQ011348 ==============================End of - Headers==============================
Having once started a similar discussion let me put two cents in.
Inspection of the periodic chart indicate N2 should be lighter than air at the same temperature and pressure. After all, air is just nitrogen contaminated with heaver gasses like O2, CO2, hydrocarbons, H2O, noble gases and only a really good analytical chemist knows what else. The point is the LN2 as it evaporates or leaks out under pressure is colder than anyone's room temperature, including that -40 degree room you're working in. Colder is denser.
How fast it warms up and the effect of gas diffusion are topics beyond my background.
Based on past discussions, I am now convinced that a large spill of liquid N in a smallish room will ruin the vinyl floor tiles, cause serious injury to you and cost you a pretty penny to repair/replace the dewar. It may also end your life.
Leaking gases should be taken seriously.
stay safe...... Frank
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==============================Original Headers============================== 8, 22 -- From frank_karl-at-lincolnelectric.com Tue May 26 14:14:09 2009 8, 22 -- Received: from lincolnelectric.com (smtp1.lincolnelectric.com [64.109.211.114]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QJE9Wq026305 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 14:14:09 -0500 8, 22 -- In-Reply-To: {200905261827.n4QIR74K008389-at-ns.microscopy.com} 8, 22 -- Subject: two more cents! - Nitrogen leak 8, 22 -- To: vladislav_speransky-at-nih.gov, Microscopy-at-microscopy.com 8, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 8, 22 -- Message-ID: {OF379CE9F1.8FD458BC-ON852575C2.0067E866-852575C2.0069A284-at-lincolnelectric.com} 8, 22 -- Date: Tue, 26 May 2009 15:13:56 -0400 8, 22 -- From: Frank_Karl-at-lincolnelectric.com 8, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 05/26/2009 03:13:52 PM, 8, 22 -- CD-MIME complete at 05/26/2009 03:13:52 PM, 8, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 05/26/2009 03:13:52 PM, 8, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 22 -- 07, 2008) at 05/26/2009 03:13:52 PM, 8, 22 -- Serialize complete at 05/26/2009 03:13:52 PM 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: text/plain; 8, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
On May 26, 2009, at 7:07 AM, nizets2-at-yahoo.com wrote:
} So I send this message in the bottle to the list. This is a call to } anybody in the field who could help me devise a protocol, or simply } send me a copy of the above paper.
Dear Stephane, If you have the citation, I, or someone else from the list, could attach a PDF of the paper to you. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Tue May 26 15:05:30 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QK5TlF009979 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 15:05:30 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 7FE0A2E50B2A 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:05:29 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 84BD92E50E41 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:05:28 -0700 (PDT) 6, 22 -- Message-Id: {A82D9DE8-5A08-4C9E-B595-07EFA815599D-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200905261407.n4QE7tp6024513-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3) 6, 22 -- Subject: Re: [Microscopy] The Redox properties of minerals 6, 22 -- Date: Tue, 26 May 2009 13:05:27 -0700 6, 22 -- References: {200905261407.n4QE7tp6024513-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.935.3) ==============================End of - Headers==============================
I am looking for someone in the Florida Tampa Bay area to work on, clean, and repair an Olympus BX40 dark field microscope. Phone 813 220 4522, secret-at-mynikken.net
Please reply off list. Thank you.
Maurice Loeb
==============================Original Headers============================== 4, 18 -- From randerson20-at-tampabay.rr.com Tue May 26 18:53:09 2009 4, 18 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4QNr9wT002726 4, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 May 2009 18:53:09 -0500 4, 18 -- Received: from [127.0.0.1] (really [24.73.73.214]) 4, 18 -- by hrndva-omta03.mail.rr.com with ESMTP 4, 18 -- id {20090526235308830.GJNL15928-at-hrndva-omta03.mail.rr.com} 4, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 May 2009 23:53:08 +0000 4, 18 -- Message-ID: {4A1C80E0.30709-at-tampabay.rr.com} 4, 18 -- Date: Tue, 26 May 2009 19:53:04 -0400 4, 18 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 4, 18 -- Reply-To: secret-at-mynikken.net 4, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 4, 18 -- MIME-Version: 1.0 4, 18 -- To: Listserver {Microscopy-at-Microscopy.Com} 4, 18 -- Subject: Looking for light microscope service in Florida 4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both shea.miller-at-agr.gc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: shea.miller-at-agr.gc.ca Name: Shea Miller
Organization: Agriculture & AgriFood Canada
Title-Subject: [Filtered] Light microscopy: localization of peroxidase in plants
Question: Hello all; I have been trying to localize peroxidase in soybean seed coats, and am wondering if anyone has a favourite protocol. The one I have been using (chloronaphthol reagent) is a bit hit and miss, and I am open to trying something new.
I looked up the structure for this Cydex-OPA. Does not really look like a good cross-linker. It is a benzene ring with two aldehyde groups attached right next to each other. Quite different from that of GA, which is a linear (flexible!) chain with an aldehyde group on each end. Cydex-OPA molecule is bigger and less flexible. Organic Chemistry was taken long time ago and never really needed, so I can't be fully trusted here, but it appears that all bonds in Cydex-OPA can be shared, stabilizing the whole structure - resonance, they call it in English. Like in phenol. Also, the aldehyde groups are too close together, another possible problem. All this considered, such molecule will want to cross-link much less than GA - this is not even considering how well it penetrates the structure.
It is a disinfectant? Sure, but so is phenol, which happens to be quite similar in structure. Looking for the structural formula, I also saw tons of disinfection references (and no cross-linking ones). Well, from the point of view of whoever is pushing for that, going Green is big right now, them bureaucrats they have all these "initiatives", "roadmaps", you name it. If someone manages to eradicate GA from a hospital, they'd score pretty big on that BS scale, I imagine.
Why doesn't somebody simply get a little of that stuff and tries fix a tissue with it? I gave reasons above not too waste time on that, but a few pictures will be a far stronger argument.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
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} From: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} } Date: May 26, 2009 9:03:22 PM EDT } To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com} } Subject: [Microscopy] TEM: ortho-phthalaldehyde } Reply-To: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Collective, } } Okay, here's a weird one. Has anyone heard of, or better yet, had any } experience with, using ortho-phthalaldehyde as a substitute for } glutaraldehyde in EM fixation? Cydex-OPA, in particular? } } Seems one of our local hospitals is kicking about their researchers } fixing tissues for EM in glut-based fixatives and is pushing them hard } to find a substitute. My searches turn this up as a disinfectant, but } our director says it theoretically might work, based on the fact that } it's a dialdehyde. } } Could this be the next great breakthrough in TEM? } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } } ==============================Original } Headers============================== } 9, 27 -- From TindallR-at-missouri.edu Tue May 26 13:49:36 2009 } 9, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n4QInaYQ011348 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:49:36 } -0500 } 9, 27 -- X-IronPort-Anti-Spam-Filtered: true } 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPPWG0rRauUp/2dsb2JhbAC/ } AQEJhmCIToJWgTUF } 9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) } ([209.106.229.41]) } 9, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 26 May } 2009 13:49:36 -0500 } 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu } ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 9, 27 -- Tue, 26 May 2009 13:49:36 -0500 } 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 27 -- Content-class: urn:content-classes:message } 9, 27 -- MIME-Version: 1.0 } 9, 27 -- Content-Type: text/plain; } 9, 27 -- charset="us-ascii" } 9, 27 -- Subject: TEM: ortho-phthalaldehyde } 9, 27 -- Date: Tue, 26 May 2009 13:49:35 -0500 } 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8113-at-UM-XMAIL08.um.umsystem.edu } } } 9, 27 -- X-MS-Has-Attach: } 9, 27 -- X-MS-TNEF-Correlator: } 9, 27 -- Thread-Topic: TEM: ortho-phthalaldehyde } 9, 27 -- Thread-Index: AcneMrcC1u+u63FzQ4q+V006CgKFUw== } 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 9, 27 -- To: {microscopy-at-microscopy.com} } 9, 27 -- X-OriginalArrivalTime: 26 May 2009 18:49:36.0075 (UTC) } FILETIME=[B73D51B0:01C9DE32] } 9, 27 -- Content-Transfer-Encoding: 8bit } 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4QInaYQ011348 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From vladislav_speransky-at-nih.gov Tue May 26 23:31:26 2009 8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R4VQWV012789 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 23:31:26 -0500 8, 23 -- Received: from compute2.internal (compute2.internal [10.202.2.42]) 8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id B4D183463BE 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:25 -0400 (EDT) 8, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161]) 8, 23 -- by compute2.internal (MEProxy); Wed, 27 May 2009 00:31:25 -0400 8, 23 -- X-Sasl-enc: 0MIB3HGArrmbARYulZUbPptk8oXL0KbhZsh08gaeYQuP 1243398685 8, 23 -- Received: from [192.168.1.5] (pool-173-79-181-192.washdc.fios.verizon.net [173.79.181.192]) 8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id B55A2463C6 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:24 -0400 (EDT) 8, 23 -- Message-Id: {8BAEDE10-0513-4244-BC16-6AA07CD2C1B9-at-nih.gov} 8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 8, 23 -- Subject: Fwd: [Microscopy] TEM: ortho-phthalaldehyde 8, 23 -- Date: Wed, 27 May 2009 00:31:22 -0400 8, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2563D18B-at-NIHMLBX02.nih.gov} 8, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
Dear Shea, Despite I am not quite confident about similarity of peroxidase activity (-ies) in plants compared with animal tissues, I would like to point you to trying 3'3'-DAB (diamino-benzidine) as a medium for localization of peroxidase.
There is a whole issue of Histochem Cell Biol (2009) 131 No 4 dealing more/less with peroxisomal issues... ==} http://springerlink.com/content/x06832162703/
The Editorial was written by: H. Dariush Fahimi Peroxisomes: 40 years of histochemical staining, personal reminiscences
Histochem Cell Biol (2009) 131:437-440, DOI 10.1007/s00418-009-0562-8 Abstract: The historical circumstances that led to the discovery of the 3,3-diamino-benzidine (DAB) method for staining of peroxisomes 40 years ago are reviewed. In the course of studies on the uptake and absorption of horse radish peroxidase in mammalian liver, in sections incubated for detection of peroxidase activity in DAB, it was noted that peroxisomes also stained positively for peroxidase activity. Subsequently, it was revealed that the peroxidatic activity of catalase, which is abundantly present in peroxisomes, is responsible for that staining. This notion was confirmed in quantitative biochemical studies with crystalline beef liver catalase and in tracer studies using catalase as an ultrastructural tracer. The application of the DAB method led to the discovery of peroxisomes as a ubiquitous eukaryotic cell organelle, attracting great interest in their investigation in biomedical research.
Perhaps an entry,
Good luck and best wishes
Wolfgang MUSS EM-Lab, Pathology, SALK-PMU (Gen.Hosp.) SALZBURG-Austria
} -----Ursprüngliche Nachricht----- } Von: shea.miller-at-agr.gc.ca [mailto:shea.miller-at-agr.gc.ca] } Gesendet: Mittwoch, 27. Mai 2009 02:17 } An: Muß Wolfgang } Betreff: [Microscopy] LM: localization of peroxidase in plants } } } -------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } -------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both shea.miller-at-agr.gc.ca as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } Email: shea.miller-at-agr.gc.ca } Name: Shea Miller } Organization: Agriculture & AgriFood Canada } Title-Subject: Light } microscopy: localization of peroxidase in plants } Question: } } Hello all; } I have been trying to localize peroxidase in soybean seed } coats, and am wondering if anyone has a favourite protocol. } The one I have been using (chloronaphthol reagent) is a bit } hit and miss, and I am open to trying something new. } } thanks in advance } shea } } Login Host: 192.197.71.189
} ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Tue May 26 19:14:43 2009 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R0EfTB017709 } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 19:14:42 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240800c642365f91af-at-[206.69.208.22]} } 7, 11 -- Date: Tue, 26 May 2009 19:14:41 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: shea.miller-at-agr.gc.ca (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Light microscopy: localization of peroxidase in plants } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 16, 36 -- From W.Muss-at-salk.at Wed May 27 01:51:56 2009 16, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R6ptq5019121 16, 36 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 01:51:56 -0500 16, 36 -- Received: from localhost (localhost [127.0.0.1]) 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 6805DC386F; 16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST) 16, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 16, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 16, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 16, 36 -- with ESMTP id NnUaKMTIuB24; Wed, 27 May 2009 08:51:54 +0200 (CEST) 16, 36 -- Received: from n2rz123.lksdom21.lks.local (n2rz123.lksdom21.lks.local [192.168.101.123]) 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 00F04C386E; 16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST) 16, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 16, 36 -- Wed, 27 May 2009 08:51:53 +0200 16, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 36 -- Content-class: urn:content-classes:message 16, 36 -- MIME-Version: 1.0 16, 36 -- Content-Type: text/plain; 16, 36 -- charset="iso-8859-1" 16, 36 -- Subject: [Microscopy] Re: LM: localization of peroxidase in plants 16, 36 -- Date: Wed, 27 May 2009 08:51:53 +0200 16, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0A8E-at-N1RZ116.lksdom21.lks.local} 16, 36 -- In-Reply-To: {200905270017.n4R0HRhS023239-at-ns.microscopy.com} 16, 36 -- X-MS-Has-Attach: 16, 36 -- X-MS-TNEF-Correlator: 16, 36 -- Thread-Topic: [Microscopy] Re: LM: localization of peroxidase in plants 16, 36 -- Thread-Index: AcneYIpjXDYR3Wv0Q7ugkr2wHDdWZwANe1Lw 16, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 16, 36 -- To: {shea.miller-at-agr.gc.ca} 16, 36 -- Cc: {microscopy-at-microscopy.com} 16, 36 -- X-OriginalArrivalTime: 27 May 2009 06:51:53.0972 (UTC) FILETIME=[9EA39B40:01C9DE97] 16, 36 -- X-Scanned-By: SALK-Content-Filter 16, 36 -- Content-Transfer-Encoding: 8bit 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4R6ptq5019121 ==============================End of - Headers==============================
I'm sorry but there are too many "mosts" and "probablys" in your argument and to even say that oxygen content is approaching death zone levels makes the basic assumption that any victim is a fit acclimatized mountain climber to be relatively safe.
Risk assessment must look at the worst scenario anyway and assume that someone may enter the room after the noisy leakage event occurred. They would have no warning and would almost certainly be unaware of what was happening to them. They might be the cleaner or an inexperienced student and be in the room alone before/after anybody else is in the building.
People do die in workplace/lab situations from oxygen depletion and there is always the risk that rescue attempts can put more people at risk.
So I would say do the risk assessment for your workplace looking at the worst leakage and find out what would be considered a safe and acceptable level of oxygen (certainly not as low as 12%). If your lab falls below those requirements then do something such as better ventilation, smaller source of gas or an oxygen depletion monitor with an alarm.
Cheers
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: dac-at-research.umass.edu
==============================Original Headers============================== 12, 27 -- From malcolm.haswell-at-sunderland.ac.uk Wed May 27 03:49:32 2009 12, 27 -- Received: from max2.sunderland.ac.uk (max2.sunderland.ac.uk [157.228.98.76]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4R8nVeO018508 12, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 03:49:31 -0500 12, 27 -- Received: (qmail 1804 invoked from network); 27 May 2009 08:44:26 -0000 12, 27 -- Received: from localhost (127.0.0.1) 12, 27 -- by max2.sunderland.ac.uk with SMTP; 27 May 2009 08:44:26 -0000 12, 27 -- Received: (qmail 1742 invoked by uid 599); 27 May 2009 08:44:20 -0000 12, 27 -- Received: from unknown (HELO hermes.sunderland.ac.uk) (157.228.37.117) 12, 27 -- by max2.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed, 27 May 2009 09:44:20 +0100 12, 27 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk (mshttpd); Wed, 12, 27 -- 27 May 2009 09:44:21 +0100 12, 27 -- Date: Wed, 27 May 2009 09:44:21 +0100 12, 27 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} 12, 27 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a problem?) 12, 27 -- To: Microscopy MSA {Microscopy-at-microscopy.com} 12, 27 -- Cc: dac-at-research.umass.edu 12, 27 -- Message-id: {241418243950.243950241418-at-sunderland.ac.uk} 12, 27 -- MIME-version: 1.0 12, 27 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004) 12, 27 -- Content-type: text/plain; charset=us-ascii 12, 27 -- Content-language: en 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- Content-disposition: inline 12, 27 -- X-Accept-Language: en 12, 27 -- Priority: normal 12, 27 -- X-Virus-Scanned: by iCritical at max2.sunderland.ac.uk ==============================End of - Headers==============================
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Email: jberrim-at-nimr.mrc.ac.uk Name: John Berriman
Title-Subject: [Filtered] Nitrogen leak (is this really a problem?)
Question: In situations where compressed gas has to be used in an enclosed space why not use air rather than nitrogen?
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Email: wvrenter-at-sckcen.be Name: Wouter Van Renterghem
Organization: SCKïCEN
Title-Subject: [Filtered] Covered TEM specimen holder
Question: My institute has a JEOL 3010 TEM and I want to investigate specimens which may not be exposed to air. The problem is not that I need to protect my specimen from air, but that I need to guarantee that at all times my specimen will not contaminate the environment. I can mount the specimen on a TEM holder in a glove box, but I am looking for a system to cover the specimen during transport and mounting in the microscope.
Does there exist a TEM holder in which the specimen is entirely encapsulated? Or is there some kind of cask which can be placed aroud the specimen tip and that can be connected to the microscope?
Has somebody worked with specimens that could not be exposed to air and can you tell how exposure was avoided?
I am also skeptic. The presence of 2 aldhehyde groups is not sufficient to consider a molecule to be a good fixative. What about the penetration? The speed of penetration? The solubility? The compatibility with biological buffers, with biological material, with stains, with processing, with resins? What about the stability/reversability of the reactions? What about the pH and temperature-dependence of the reaction? Theory is nice, but we still have found no alternative to practical testing for definitive assessment. Please let us know how it worked :-).
On a side note, a benzene ring doesn't look particularly healthy in my mind of biologist. Is this product really so safe/so much safer than Glutar?
Regards,
Stephane
----- Original Message ---- X-from: "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov} To: nizets2-at-yahoo.com Sent: Wednesday, May 27, 2009 6:35:55 AM
I looked up the structure for this Cydex-OPA. Does not really look like a good cross-linker. It is a benzene ring with two aldehyde groups attached right next to each other. Quite different from that of GA, which is a linear (flexible!) chain with an aldehyde group on each end. Cydex-OPA molecule is bigger and less flexible. Organic Chemistry was taken long time ago and never really needed, so I can't be fully trusted here, but it appears that all bonds in Cydex-OPA can be shared, stabilizing the whole structure - resonance, they call it in English. Like in phenol. Also, the aldehyde groups are too close together, another possible problem. All this considered, such molecule will want to cross-link much less than GA - this is not even considering how well it penetrates the structure.
It is a disinfectant? Sure, but so is phenol, which happens to be quite similar in structure. Looking for the structural formula, I also saw tons of disinfection references (and no cross-linking ones). Well, from the point of view of whoever is pushing for that, going Green is big right now, them bureaucrats they have all these "initiatives", "roadmaps", you name it. If someone manages to eradicate GA from a hospital, they'd score pretty big on that BS scale, I imagine.
Why doesn't somebody simply get a little of that stuff and tries fix a tissue with it? I gave reasons above not too waste time on that, but a few pictures will be a far stronger argument.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and do not represent the NIH. On the good side, this message is not confidential and can be freely shared and reproduced.
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} From: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} } Date: May 26, 2009 9:03:22 PM EDT } To: "vlad_speransky-at-me.com" {vlad_speransky-at-me.com} } Subject: [Microscopy] TEM: ortho-phthalaldehyde } Reply-To: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Collective, } } Okay, here's a weird one. Has anyone heard of, or better yet, had any } experience with, using ortho-phthalaldehyde as a substitute for } glutaraldehyde in EM fixation? Cydex-OPA, in particular? } } Seems one of our local hospitals is kicking about their researchers } fixing tissues for EM in glut-based fixatives and is pushing them hard } to find a substitute. My searches turn this up as a disinfectant, but } our director says it theoretically might work, based on the fact that } it's a dialdehyde. } } Could this be the next great breakthrough in TEM? } } Cheers, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } Sons of Norway: http://www.sofn.com } } } } } ==============================Original } Headers============================== } 9, 27 -- From TindallR-at-missouri.edu Tue May 26 13:49:36 2009 } 9, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu } (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id n4QInaYQ011348 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 13:49:36 } -0500 } 9, 27 -- X-IronPort-Anti-Spam-Filtered: true } 9, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPPWG0rRauUp/2dsb2JhbAC/ } AQEJhmCIToJWgTUF } 9, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) } ([209.106.229.41]) } 9, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 26 May } 2009 13:49:36 -0500 } 9, 27 -- Received: from UM-XMAIL08.um.umsystem.edu } ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 9, 27 -- Tue, 26 May 2009 13:49:36 -0500 } 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 27 -- Content-class: urn:content-classes:message } 9, 27 -- MIME-Version: 1.0 } 9, 27 -- Content-Type: text/plain; } 9, 27 -- charset="us-ascii" } 9, 27 -- Subject: TEM: ortho-phthalaldehyde } 9, 27 -- Date: Tue, 26 May 2009 13:49:35 -0500 } 9, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8113-at-UM-XMAIL08.um.umsystem.edu } } } 9, 27 -- X-MS-Has-Attach: } 9, 27 -- X-MS-TNEF-Correlator: } 9, 27 -- Thread-Topic: TEM: ortho-phthalaldehyde } 9, 27 -- Thread-Index: AcneMrcC1u+u63FzQ4q+V006CgKFUw== } 9, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 9, 27 -- To: {microscopy-at-microscopy.com} } 9, 27 -- X-OriginalArrivalTime: 26 May 2009 18:49:36.0075 (UTC) } FILETIME=[B73D51B0:01C9DE32] } 9, 27 -- Content-Transfer-Encoding: 8bit } 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4QInaYQ011348 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From vladislav_speransky-at-nih.gov Tue May 26 23:31:26 2009 8, 23 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4R4VQWV012789 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 26 May 2009 23:31:26 -0500 8, 23 -- Received: from compute2.internal (compute2.internal [10.202.2.42]) 8, 23 -- by out1.messagingengine.com (Postfix) with ESMTP id B4D183463BE 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:25 -0400 (EDT) 8, 23 -- Received: from heartbeat2.messagingengine.com ([10.202.2.161]) 8, 23 -- by compute2.internal (MEProxy); Wed, 27 May 2009 00:31:25 -0400 8, 23 -- X-Sasl-enc: 0MIB3HGArrmbARYulZUbPptk8oXL0KbhZsh08gaeYQuP 1243398685 8, 23 -- Received: from [192.168.1.5] (pool-173-79-181-192.washdc.fios.verizon.net [173.79.181.192]) 8, 23 -- by mail.messagingengine.com (Postfix) with ESMTPA id B55A2463C6 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 00:31:24 -0400 (EDT) 8, 23 -- Message-Id: {8BAEDE10-0513-4244-BC16-6AA07CD2C1B9-at-nih.gov} 8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- Mime-Version: 1.0 (Apple Message framework v930.3) 8, 23 -- Subject: Fwd: [Microscopy] TEM: ortho-phthalaldehyde 8, 23 -- Date: Wed, 27 May 2009 00:31:22 -0400 8, 23 -- References: {D13763A4740B07428566B25D7E768DA60B2563D18B-at-NIHMLBX02.nih.gov} 8, 23 -- X-Mailer: Apple Mail (2.930.3) ==============================End of - Headers==============================
==============================Original Headers============================== 25, 24 -- From nizets2-at-yahoo.com Wed May 27 08:27:51 2009 25, 24 -- Received: from web110816.mail.gq1.yahoo.com (web110816.mail.gq1.yahoo.com [67.195.13.239]) 25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4RDRoAb009077 25, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 08:27:50 -0500 25, 24 -- Received: (qmail 30902 invoked by uid 60001); 27 May 2009 13:27:48 -0000 25, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243430868; bh=AOjj9BscDZf0NTJUmfx588AejiwIakjCKk1Niam7N6c=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=BB3qUovinH1kAfIidCTaNq73RJxq8y4yQZ0hdQSGt9v89zx6n6OAnz9PZA/VhLCYGYGgTXNdoxlKdrzRBlV5yNbg4o5RTJRfBJmZdUenV1Vj9bnvtEim/U+HFQ1zOCtQ7jZ2e3eicz5INst6+jHxGnQ4/KgC9g0Bknn7z0N+S/M= 25, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 25, 24 -- s=s1024; d=yahoo.com; 25, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 25, 24 -- b=W4SCkGfgao7bNXZI7tdV21vKwhJb1MbX1yvAsh4wpytMeRM5Yx7bWtzBtMNTNlZCwEDNmSwNH73vSSsylod71YukOfdgjpB1J6LL48236NBhjHZ5knF4dO+lPatCXonHIUO+xLEWceZ9zv10wDySoo9qbxft/8WD2jYqqvMrGtw=; 25, 24 -- Message-ID: {634488.30550.qm-at-web110816.mail.gq1.yahoo.com} 25, 24 -- X-YMail-OSG: PKh7EG4VM1nxITvl6yszt5uIXVqMBr8t8.sNpzSjgf.KwZG4oH2tsqWXD0O4ioTWzQvrpRjfpZl2zJwoo8BE9XbmoV4_cumFYyVHpaIa2KnM5LZD9aN_INyNM.IYEFbbwe4KH5d5ZW6k4n5Vwmpkaq.QLU37DjX24y_7g5tldrYfyeILuGb_qsKpGZfZGGQ42zXOaZRwFRLytteLoA9uS710jISjSpIJJ34s0LfxkvZ6XdH5.RkkDh7Lwx65tpCHMzeOHOnKlFCimcyoWZb0NMQ7DialMNgeNRYaueyGI7xCGvtfIED91u.Dl46ZOMX0IoXksFDYmXBJI4Itov9diyRP 25, 24 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Wed, 27 May 2009 06:27:48 PDT 25, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10 25, 24 -- References: {200905270435.n4R4ZtEp019244-at-ns.microscopy.com} 25, 24 -- Date: Wed, 27 May 2009 06:27:48 -0700 (PDT) 25, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com} 25, 24 -- Subject: Re: [Microscopy] Fwd: TEM: ortho-phthalaldehyde 25, 24 -- To: microscopy-at-microscopy.com 25, 24 -- In-Reply-To: {200905270435.n4R4ZtEp019244-at-ns.microscopy.com} 25, 24 -- MIME-Version: 1.0 25, 24 -- Content-Type: text/plain; charset=iso-8859-1 25, 24 -- Content-Transfer-Encoding: 8bit 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4RDRoAb009077 ==============================End of - Headers==============================
We have been successful using a glove bag instead of a glove box and filling it with Argon. The specimen is loaded into the holder in the glove bag and then with the help of other researchers is offered up to the microscope airlock in the bag with a slight positive pressure of Argon. The bag is opened slightly to enclose the airlock and the holder loaded into the airlock. Once the pumping switches over to high vacuum the glove bag can be removed.
This has also been successfully used to load reduced catalyst specimens into an XPS with no measurable oxidation.
Regards
Alan
At 07:55 AM 5/27/2009, you wrote:
} Email: wvrenter-at-sckcen.be } Name: Wouter Van Renterghem } } Organization: SCKïCEN } } Title-Subject: [Filtered] Covered TEM specimen holder } } Question: My institute has a JEOL 3010 TEM and I } want to investigate specimens which may not be } exposed to air. The problem is not that I need } to protect my specimen from air, but that I need } to guarantee that at all times my specimen will } not contaminate the environment. I can mount the } specimen on a TEM holder in a glove box, but I am } looking for a system to cover the specimen during } transport and mounting in the microscope. } } Does there exist a TEM holder in which the } specimen is entirely encapsulated? Or is there } some kind of cask which can be placed aroud the } specimen tip and that can be connected to the } microscope? } } Has somebody worked with specimens that could not } be exposed to air and can you tell how exposure } was avoided? } } Thank you } Wouter Van Renterghem } } Login Host: 193.190.187.220 } --------------------------------------------------------------------------- } }
Alan W Nicholls, PhD Interim Associate Director - RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Let's assume a six foot wide fume hood with a bypass grill 'overhead', has a face velocity of 100 feet per minute, and a closed sash opening area of 12 square feet. That means that the cubic feet per minute of air flow is 1200 CFM. Let's use a 12 x 12 x 10 foot room that has a HVAC system of air turnover. That room has a volume of 1440 cubic feet. So a hood outside this room might be supplied by as many as three inlet air vents. So the air turnover in the leaking nitrogen room could be as low as about 480 CFM. So after three minutes, the volume of normal air forced into the room is equal to the volume of the room.
It takes five volumes of a gas to flush out all the collected gas in a gas collection bulb. Let's assume that is the correct volume needed for a lab of volume 1440 CF. Five volumes circulated without a leak is 7200 CF. 7200/1200 = 6 minutes (for five volumes of room air). Now let's displace some of that air with nitrogen. Let's lower the O2 level to about 16% from 20%. The dilution formula is C1*V1 = C2*V2. (20%)(V1) = (16%)(7200). V1 = 5760 CF of air. 7200 - 5760 = 1440 CF of extra nitrogen is needed in six minutes. That's means 240 CFM of nitrogen leakage is needed to create a 16% O2 atmosphere from adding pure nitrogen to the HVAC air containing 20% O2 every minute.
240 CFM is a leakage rate of 6,796 liters of nitrogen per minute. That's a huge nitrogen leak for a pinhole in an air table bladder or a Philips EM column bladder. Considering your N2 cylinder has only 225 CF per tank, one would have to empty more than one cylinder per minute, probably without a regulator! The nitrogen will be compressed in the bladder but once it leaks out, it is 240 CFM.
That leakage rate might be a fairly noisy gas leak for an air table, IMO.
"(is this really a problem?)" Not in my opinion.
Paul
At 10:17 AM 5/26/09 -0500, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 27 -- From beaurega-at-westol.com Wed May 27 10:04:56 2009 9, 27 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.96.4]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RF4tcE009484 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 10:04:55 -0500 9, 27 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 9, 27 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id n4RF4if3025800 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 11:04:46 -0400 9, 27 -- Received: (qmail 20516 invoked by uid 89); 27 May 2009 13:17:59 -0000 9, 27 -- Received: from pitts-69-72-117-93.dynamic-dialup.coretel.net (HELO running) (69.72.117.93) 9, 27 -- by mail.winbeam.com with SMTP; 27 May 2009 13:17:59 -0000 9, 27 -- Message-Id: {3.0.6.32.20090527091735.0085f100-at-pop3.norton.antivirus} 9, 27 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 9, 27 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 9, 27 -- Date: Wed, 27 May 2009 09:17:35 -0400 9, 27 -- To: microscopy-at-microscopy.com 9, 27 -- From: Beaurega {beaurega-at-westol.com} 9, 27 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a 9, 27 -- problem?) 9, 27 -- Mime-Version: 1.0 9, 27 -- Content-Type: text/plain; charset="us-ascii" 9, 27 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 9, 27 -- X-Winbeam-MailScanner-ID: n4RF4if3025800 9, 27 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 9, 27 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 9, 27 -- SpamAssassin (not cached, score=-1.759, required 4, 9, 27 -- autolearn=not spam, AWL 0.16, BAYES_00 -2.00, TW_FT 0.08) 9, 27 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
We have the SampleSaver(TM) Storage Container that will store and transport samples in a slightly pressurized inert atmosphere. Here is a link to information on the units, http://www.southbaytech.com/pdfs/SS1.pdf. We have different racks including one that will store TEM grids. This unit was designed for preventing oxidation of samples, but it would also be a solution for you. They can be used quite easily with a glove bag or a glove box. When you do use a bag or box, you do not need to purge the container as they are normally used. I saw that Alan Nicholls already discussed using a glove bag to load the TEM holder and into the TEM airlock. I would use N2 instead of Ar because of the expense and the possibility of using house N2 if you have it.
You might also look into some of the cryogenic holders. They can load the sample under a N2 atmosphere and some have a little slide cover that protects the sample during transport from the cyro-workstation to the microscope. I know that Gatan has a system like that. Here is a link to such a holder on their site: http://gatan.com/products/specimen_holders/products/CT3500TR_CryoTransferTil tRotate.php.
Disclaimer: SBT manufactures and sells the SampleSaver(TM) Storage Container.
-Scott
Scott D. Walck, Ph.D. Technical Director
South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
-----Original Message----- X-from: wvrenter-at-sckcen.be [mailto:wvrenter-at-sckcen.be] Sent: Wednesday, May 27, 2009 6:06 AM To: swalck-at-southbaytech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wvrenter-at-sckcen.be as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wvrenter-at-sckcen.be Name: Wouter Van Renterghem
Organization: SCKïCEN
Title-Subject: [Filtered] Covered TEM specimen holder
Question: My institute has a JEOL 3010 TEM and I want to investigate specimens which may not be exposed to air. The problem is not that I need to protect my specimen from air, but that I need to guarantee that at all times my specimen will not contaminate the environment. I can mount the specimen on a TEM holder in a glove box, but I am looking for a system to cover the specimen during transport and mounting in the microscope.
Does there exist a TEM holder in which the specimen is entirely encapsulated? Or is there some kind of cask which can be placed aroud the specimen tip and that can be connected to the microscope?
Has somebody worked with specimens that could not be exposed to air and can you tell how exposure was avoided?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both astrohsc-at-aerotek.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: astrohsc-at-aerotek.com Name: Andrew Strohschein
Organization: Aerotek Scientific
Title-Subject: [Filtered] Immediate need for an SEM Operator
Question: Aerotek Scientific is looking for an SEM Operator to work on a project basis through the end of the year at a Minnesota based medical device company.
Qualified candidates need to have experience with SEM-EDS. This person will be performing material verification / material composition, failure analysis on medical devices.
Interested candidates should send resumes to astrohsc-at-aerotek.com or contact Andrew directly at 763-852-1349.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hong_s-at-palmer.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hong_s-at-palmer.edu Name: Se Pyo Hong
Organization: Graduate Studies at Palmer College of Chiropractic
Title-Subject: [Filtered] reembedment of a semithin section
Question: Hi everyone; I have 2-micron thick flat specimens (cross sectioned rat spinal cord) embedded in epon with toluidine stain on a glass slide. I want to have ultrathin sections of part of the specimen (Raxed lamina 1 - 3 area). Please give me suggestions or a procedure. Thank you..
I have designed specimen rods to protect specimens from the atmosphere during transfer from a glove box to the microscope which have performed successfully in both a JEOL 2010 TEM and a JEOL HF-2200 aberration-corrected TEM. I believe the specimen rod for the 3010 is very similar to these others, and so a specimen rod of this design should work for you. Let me know if you want more information -- -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 15 -- From bigelow-at-umich.edu Wed May 27 14:50:18 2009 1, 15 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 1, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RJoIhO015434 1, 15 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 14:50:18 -0500 1, 15 -- Received: FROM [141.212.131.153] (bigelow-imac-g5.engin.umich.edu [141.212.131.153]) 1, 15 -- By skycaptain.mr.itd.umich.edu ID 4A1D9979.C4FA8.8883 ; 1, 15 -- Authuser bigelow; 1, 15 -- 27 May 2009 15:50:17 EDT 1, 15 -- Mime-Version: 1.0 1, 15 -- Message-Id: {p06240800c643495659e5-at-[141.212.131.153]} 1, 15 -- Date: Wed, 27 May 2009 15:50:16 -0400 1, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 15 -- Subject: [Microscopy]RE;Covered spec rod 1, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
You can certainly use DAB to detect ROX in plants, also a fluorescent probe, H2DCFDA to detect various oxygen radicals - results of peroxidase activity, I guess, and there are others to detect H2O2 as well - a number of new probes have been synthesised lately.
cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 27/05/09 4:58 PM, "W.Muss-at-salk.at" {W.Muss-at-salk.at} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning, } hello all, } } Dear Shea, } Despite I am not quite confident about similarity of peroxidase activity } (-ies) in plants compared with animal tissues, I would like to point you to } trying 3'3'-DAB (diamino-benzidine) as a medium for localization of } peroxidase. } } There is a whole issue of Histochem Cell Biol (2009) 131 No 4 dealing } more/less with peroxisomal issues... } ==} http://springerlink.com/content/x06832162703/ } } The Editorial was written by: } H. Dariush Fahimi } Peroxisomes: 40 years of histochemical staining, personal reminiscences } } Histochem Cell Biol (2009) 131:437-440, DOI 10.1007/s00418-009-0562-8 } Abstract: The historical circumstances that led to the discovery of the } 3,3-diamino-benzidine (DAB) method for } staining of peroxisomes 40 years ago are reviewed. In the course of studies on } the uptake and absorption of horse radish peroxidase in mammalian liver, in } sections incubated for detection of peroxidase activity in DAB, it was noted } that peroxisomes also stained positively for peroxidase activity. } Subsequently, it was revealed that the peroxidatic activity of catalase, which } is abundantly present in peroxisomes, is responsible for that staining. This } notion was confirmed in quantitative biochemical studies with crystalline beef } liver catalase and in tracer studies using catalase as an ultrastructural } tracer. The application of the DAB method led to the discovery of peroxisomes } as a ubiquitous eukaryotic cell organelle, attracting great interest in their } investigation in biomedical research. } } Perhaps an entry, } } Good luck and best wishes } } Wolfgang MUSS } EM-Lab, Pathology, SALK-PMU (Gen.Hosp.) } SALZBURG-Austria } } } } } } } } } -----Ursprüngliche Nachricht----- } } Von: shea.miller-at-agr.gc.ca [mailto:shea.miller-at-agr.gc.ca] } } Gesendet: Mittwoch, 27. Mai 2009 02:17 } } An: Muß Wolfgang } } Betreff: [Microscopy] LM: localization of peroxidase in plants } } } } } } -------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } -------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, } } so when replying } } please copy both shea.miller-at-agr.gc.ca as well as the } } MIcroscopy Listserver } } -------------------------------------------------------------- } } Email: shea.miller-at-agr.gc.ca } } Name: Shea Miller } } Organization: Agriculture & AgriFood Canada } } Title-Subject: Light } } microscopy: localization of peroxidase in plants } } Question: } } } } Hello all; } } I have been trying to localize peroxidase in soybean seed } } coats, and am wondering if anyone has a favourite protocol. } } The one I have been using (chloronaphthol reagent) is a bit } } hit and miss, and I am open to trying something new. } } } } thanks in advance } } shea } } } } Login Host: 192.197.71.189 } } } ==============================Original Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Tue May 26 19:14:43 2009 } } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } n4R0EfTB017709 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 May 2009 19:14:42 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240800c642365f91af-at-[206.69.208.22]} } } 7, 11 -- Date: Tue, 26 May 2009 19:14:41 -0500 } } 7, 11 -- To: microscopy-at-microscopy.com } } 7, 11 -- From: shea.miller-at-agr.gc.ca (by way of MicroscopyListserver) } } 7, 11 -- Subject: viaWWW: Light microscopy: localization of peroxidase in } } plants } } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 16, 36 -- From W.Muss-at-salk.at Wed May 27 01:51:56 2009 } 16, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) } 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n4R6ptq5019121 } 16, 36 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 01:51:56 -0500 } 16, 36 -- Received: from localhost (localhost [127.0.0.1]) } 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 6805DC386F; } 16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST) } 16, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at } 16, 36 -- Received: from hermes.salk.at ([127.0.0.1]) } 16, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port } 10024) } 16, 36 -- with ESMTP id NnUaKMTIuB24; Wed, 27 May 2009 08:51:54 +0200 (CEST) } 16, 36 -- Received: from n2rz123.lksdom21.lks.local } (n2rz123.lksdom21.lks.local [192.168.101.123]) } 16, 36 -- by hermes.salk.at (Postfix) with ESMTP id 00F04C386E; } 16, 36 -- Wed, 27 May 2009 08:51:54 +0200 (CEST) } 16, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by } n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); } 16, 36 -- Wed, 27 May 2009 08:51:53 +0200 } 16, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 16, 36 -- Content-class: urn:content-classes:message } 16, 36 -- MIME-Version: 1.0 } 16, 36 -- Content-Type: text/plain; } 16, 36 -- charset="iso-8859-1" } 16, 36 -- Subject: [Microscopy] Re: LM: localization of peroxidase in plants } 16, 36 -- Date: Wed, 27 May 2009 08:51:53 +0200 } 16, 36 -- Message-ID: } {06B4ED29F824524E98E8AA5BB64070625D0A8E-at-N1RZ116.lksdom21.lks.local} } 16, 36 -- In-Reply-To: {200905270017.n4R0HRhS023239-at-ns.microscopy.com} } 16, 36 -- X-MS-Has-Attach: } 16, 36 -- X-MS-TNEF-Correlator: } 16, 36 -- Thread-Topic: [Microscopy] Re: LM: localization of peroxidase in } plants } 16, 36 -- Thread-Index: AcneYIpjXDYR3Wv0Q7ugkr2wHDdWZwANe1Lw } 16, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} } 16, 36 -- To: {shea.miller-at-agr.gc.ca} } 16, 36 -- Cc: {microscopy-at-microscopy.com} } 16, 36 -- X-OriginalArrivalTime: 27 May 2009 06:51:53.0972 (UTC) } FILETIME=[9EA39B40:01C9DE97] } 16, 36 -- X-Scanned-By: SALK-Content-Filter } 16, 36 -- Content-Transfer-Encoding: 8bit } 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4R6ptq5019121 } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 45 -- From prvs=392b67a6d=Rosemary.White-at-csiro.au Wed May 27 21:39:04 2009 12, 45 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 12, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4S2d2w9011843 12, 45 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 21:39:03 -0500 12, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 12, 45 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 12, 45 -- s=email; t=1243478343; x=1275014343; 12, 45 -- h=from:sender:reply-to:subject:date:message-id:to:cc: 12, 45 -- mime-version:content-transfer-encoding:content-id: 12, 45 -- content-description:resent-date:resent-from:resent-sender: 12, 45 -- resent-to:resent-cc:resent-message-id:in-reply-to: 12, 45 -- references:list-id:list-help:list-unsubscribe: 12, 45 -- list-subscribe:list-post:list-owner:list-archive; 12, 45 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au} 12, 45 -- |Subject:=20Re:=20[Microscopy]=20=20Re:=20LM:=20localizat 12, 45 -- ion=20of=20peroxidase=20in=20plants|Date:=20Thu,=2028=20M 12, 45 -- ay=202009=2012:38:59=20+1000|Message-ID:=20 {C6443663.6042 12, 45 -- %rosemary.white-at-csiro.au} |To:=20 {microscopy-at-microscopy.co 12, 45 -- m} |MIME-Version:=201.0|Content-Transfer-Encoding:=20quote 12, 45 -- d-printable|In-Reply-To:=20 {200905270658.n4R6wUC9029042-at-n 12, 45 -- s.microscopy.com} ; 12, 45 -- bh=kRwahgIhDs/fQvs54i2G8XaE70Ki7MAU3nuEBYgo7wI=; 12, 45 -- b=kX18N9i+Xm6q6XY/y5reNXaC38t19WLyPfFtgvN7aK9FOYxzpm5evFXI 12, 45 -- /vDX5+casKGD2XuNHb5qbuPteWGGKRClkBC/55kHPjVHF3Hp13uWPFoTN 12, 45 -- R/mBcV037I3jvUw; 12, 45 -- X-IronPort-AV: E=Sophos;i="4.41,262,1241359200"; 12, 45 -- d="scan'208";a="27520030" 12, 45 -- Received: from exnsw-htca01.nexus.csiro.au ([130.155.117.126]) 12, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 28 May 2009 12:39:01 +1000 12, 45 -- Received: from [152.83.193.49] (152.83.193.49) by EXNSW-HTCA01.nexus.csiro.au 12, 45 -- (130.155.117.126) with Microsoft SMTP Server id 8.1.358.0; Thu, 28 May 2009 12, 45 -- 12:39:00 +1000 12, 45 -- User-Agent: Microsoft-Entourage/12.10.0.080409 12, 45 -- Date: Thu, 28 May 2009 12:38:59 +1000 12, 45 -- Subject: Re: [Microscopy] Re: LM: localization of peroxidase in plants 12, 45 -- From: Rosemary White {rosemary.white-at-csiro.au} 12, 45 -- To: {microscopy-at-microscopy.com} 12, 45 -- Message-ID: {C6443663.6042%rosemary.white-at-csiro.au} 12, 45 -- Thread-Topic: [Microscopy] Re: LM: localization of peroxidase in plants 12, 45 -- Thread-Index: AcnemI1XpPjTEhxkQJqtG4t3e1HaMgApOa7g 12, 45 -- In-Reply-To: {200905270658.n4R6wUC9029042-at-ns.microscopy.com} 12, 45 -- MIME-Version: 1.0 12, 45 -- Content-Type: text/plain; charset="ISO-8859-1" 12, 45 -- Content-Transfer-Encoding: 8bit 12, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4S2d2w9011843 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both david.mitchell-at-emu.usyd.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: david.mitchell-at-emu.usyd.edu.au Name: Dave Mitchell
I have a new dry pumped TEM. The vacuum and performance is to specification and the machine is operating perfectly. The system is backed by two incredibly noisy scroll pumps. I have built a sound-proof enclosure for these and it has made a huge difference. However, in the new found hush, the whine of the two turbo pumps is noticeable. It is not excessive, it is just annoying (to me anyway). The pumps are water cooled.
Question: Has anyone attempted to quieten such pumps by wrapping them in sound absorbing material, and if so, did it make any difference?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both liina170-at-hot.ee as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: liina170-at-hot.ee Name: Liina
Organization: University of Tartu
Title-Subject: [Filtered] SEM and TEM
Question: What would be the main advantages and disadvantages of a SEM that operates upside down i.e. has e-gun at the bottom pointing upwards. What about horizontal design? The same questions about TEM.
For SEM it would be very inconvenient to mount specimens upside down, in addition all the dirt and dust will fall down in the direction of an electron gun, contaminating it. TEM column is too tall to mount it upside down, and fluorescence screen should be transparent. Horizontal positioning will take much more space than vertical one, and again will introduce inconvenience in mounting SEM specimens and observation of TEM screen.
Physics allows any positioning of a column in space; ergonomics speaks for traditional one, vertical with a gun on the top.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } Sent: Thursday, May 28, 2009 7:40 AM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } This Question/Comment was submitted to the Microscopy } Listserver using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } -------------------------------------------------------------- } ------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both liina170-at-hot.ee as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } ------------- } } Email: liina170-at-hot.ee } Name: Liina } } Organization: University of Tartu } } Title-Subject: [Filtered] SEM and TEM } } Question: What would be the main advantages and disadvantages } of a SEM that operates upside down i.e. has e-gun at the } bottom pointing upwards. What about horizontal design? The } same questions about TEM. } } } Login Host: 213.184.38.91 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n4SCcqfd009244 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } 2009 07:38:53 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM } and TEM Column Orientation 7, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.167]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SCwsIq007428 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 07:58:55 -0500 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="us-ascii" 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) FILETIME=[0D6DBFE0:01C9DF94] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SCwsIq007428 ==============================End of - Headers==============================
Not to mention that on an inverted column the electrons have to overcome gravity to make their way from the gun to the sample! {...grin...}
Henk
At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:
} For SEM it would be very inconvenient to mount specimens upside down, in } addition all the dirt and dust will fall down in the direction of an } electron gun, contaminating it. TEM column is too tall to mount it } upside down, and fluorescence screen should be transparent. Horizontal } positioning will take much more space than vertical one, and again will } introduce inconvenience in mounting SEM specimens and observation of TEM } screen. } } Physics allows any positioning of a column in space; ergonomics speaks } for traditional one, vertical with a gun on the top. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } } -----Original Message----- } } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } } Sent: Thursday, May 28, 2009 7:40 AM } } To: Dusevich, Vladimir } } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } } } } } } -------------------------------------------------------------- } } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------- } } -------------- } } } } This Question/Comment was submitted to the Microscopy } } Listserver using the WWW based Form at } } http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------- } } ------------- } } Remember this posting is most likely not from a Subscriber, } } so when replying } } please copy both liina170-at-hot.ee as well as the } } MIcroscopy Listserver } } -------------------------------------------------------------- } } ------------- } } } } Email: liina170-at-hot.ee } } Name: Liina } } } } Organization: University of Tartu } } } } Title-Subject: [Filtered] SEM and TEM } } } } Question: What would be the main advantages and disadvantages } } of a SEM that operates upside down i.e. has e-gun at the } } bottom pointing upwards. What about horizontal design? The } } same questions about TEM. } } } } } } Login Host: 213.184.38.91 } } -------------------------------------------------------------- } } ------------- } } } } ==============================Original } } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 } } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n4SCcqfd009244 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } } 2009 07:38:53 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by } } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM } } and TEM Column Orientation 7, 11 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 } 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu } (smtp.exchange.umkc.edu [134.193.143.167]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4SCwsIq007428 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 } 07:58:55 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by } kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 } 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation } 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 } 8, 25 -- Message-ID: } {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation } 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA } 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) } FILETIME=[0D6DBFE0:01C9DF94] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4SCwsIq007428 } ==============================End of - Headers==============================
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 7, 26 -- From colijn.1-at-osu.edu Thu May 28 08:19:02 2009 7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDJ0tP023075 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:19:01 -0500 7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 7, 26 -- id {01N9HBFG6Y8W8XEJHW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 7, 26 -- Thu, 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Received: from HOC1.ecr6.ohio-state.edu 7, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 7, 26 -- (PMDF V6.3-x18 #31556) 7, 26 -- with ESMTPA id {01N9HBFFWDQQ8X5P9B-at-ecr6.ohio-state.edu} ; Thu, 7, 26 -- 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Date: Thu, 28 May 2009 09:20:24 -0400 7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 26 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation 7, 26 -- In-reply-to: {200905281300.n4SD0xOu009804-at-ns.microscopy.com} 7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 26 -- To: DusevichV-at-umkc.edu 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 -- Message-id: {01N9HBFFXPS48X5P9B-at-ecr6.ohio-state.edu} 7, 26 -- MIME-version: 1.0 7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 7, 26 -- References: {200905281300.n4SD0xOu009804-at-ns.microscopy.com} ==============================End of - Headers==============================
I have seen pictures of a horizontal TEM from the early days.
Dave
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: 28 May 2009 14:23 To: David Patton
Not to mention that on an inverted column the electrons have to overcome gravity to make their way from the gun to the sample! {...grin...}
Henk
At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:
} For SEM it would be very inconvenient to mount specimens upside down, } in addition all the dirt and dust will fall down in the direction of an
} electron gun, contaminating it. TEM column is too tall to mount it } upside down, and fluorescence screen should be transparent. Horizontal } positioning will take much more space than vertical one, and again will
} introduce inconvenience in mounting SEM specimens and observation of } TEM screen. } } Physics allows any positioning of a column in space; ergonomics speaks } for traditional one, vertical with a gun on the top. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } } -----Original Message----- } } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } } Sent: Thursday, May 28, 2009 7:40 AM } } To: Dusevich, Vladimir } } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } } } } } } -------------------------------------------------------------- } } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------- } } -------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------- } } ------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both liina170-at-hot.ee as well as the } } MIcroscopy Listserver } } -------------------------------------------------------------- } } ------------- } } } } Email: liina170-at-hot.ee } } Name: Liina } } } } Organization: University of Tartu } } } } Title-Subject: [Filtered] SEM and TEM } } } } Question: What would be the main advantages and disadvantages of a } } SEM that operates upside down i.e. has e-gun at the bottom pointing } } upwards. What about horizontal design? The same questions about TEM. } } } } } } Login Host: 213.184.38.91 } } -------------------------------------------------------------- } } ------------- } } } } ==============================Original } } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 7, 11 } } -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n4SCcqfd009244 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } } 2009 07:38:53 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by way of } } MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM and TEM Column } } Orientation 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; } } format="flowed" } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original } Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 8, 25 -- } Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu } [134.193.143.167]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4SCwsIq007428 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 } 07:58:55 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by } kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 } 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 -- } Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column } Orientation 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 8, 25 -- } Message-ID: } {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column } Orientation 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA } 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: } {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} 8, 25 -- } X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) } FILETIME=[0D6DBFE0:01C9DF94] 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4SCwsIq007428 ==============================End } of - Headers==============================
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 7, 26 -- From colijn.1-at-osu.edu Thu May 28 08:19:02 2009 7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDJ0tP023075 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:19:01 -0500 7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 7, 26 -- id {01N9HBFG6Y8W8XEJHW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 7, 26 -- Thu, 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Received: from HOC1.ecr6.ohio-state.edu 7, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 7, 26 -- (PMDF V6.3-x18 #31556) 7, 26 -- with ESMTPA id {01N9HBFFWDQQ8X5P9B-at-ecr6.ohio-state.edu} ; Thu, 7, 26 -- 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Date: Thu, 28 May 2009 09:20:24 -0400 7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 26 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation 7, 26 -- In-reply-to: {200905281300.n4SD0xOu009804-at-ns.microscopy.com} 7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 26 -- To: DusevichV-at-umkc.edu 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 -- Message-id: {01N9HBFFXPS48X5P9B-at-ecr6.ohio-state.edu} 7, 26 -- MIME-version: 1.0 7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 7, 26 -- References: {200905281300.n4SD0xOu009804-at-ns.microscopy.com} ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 34 -- From David.Patton-at-uwe.ac.uk Thu May 28 08:24:46 2009 22, 34 -- Received: from mailapp03.uwe.ac.uk (mailapp03.uwe.ac.uk [164.11.132.65]) 22, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4SDOi19032193 22, 34 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:24:45 -0500 22, 34 -- Received: from (unknown [164.11.132.62]) by mailapp03.uwe.ac.uk with smtp 22, 34 -- id 55a2_e7ad0b7c_4b8a_11de_bcf6_00142221cca9; 22, 34 -- Thu, 28 May 2009 14:24:43 +0100 22, 34 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 22, 34 -- (egen-uwe01.uwe.ac.uk [164.11.249.121]) 22, 34 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 22, 34 -- 2005)) with ESMTP id {0KKC000NWVX618-at-mta02.uwe.ac.uk} for 22, 34 -- microscopy-at-microscopy.com; Thu, 28 May 2009 14:24:43 +0100 (BST) 22, 34 -- Date: Thu, 28 May 2009 14:24:23 +0100 22, 34 -- From: David Patton {David.Patton-at-uwe.ac.uk} 22, 34 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation 22, 34 -- In-reply-to: {200905281322.n4SDMjxZ028879-at-ns.microscopy.com} 22, 34 -- To: colijn.1-at-osu.edu 22, 34 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF8528A-at-egen-uwe01} 22, 34 -- MIME-version: 1.0 22, 34 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 22, 34 -- Content-type: text/plain; charset=us-ascii 22, 34 -- Content-class: urn:content-classes:message 22, 34 -- Thread-topic: [Microscopy] viaWWW: SEM and TEM Column Orientation 22, 34 -- Thread-index: Acnfl2q6izn2N4XySqCGyuJVGsyDfgAABc1g 22, 34 -- X-MS-Has-Attach: 22, 34 -- X-MS-TNEF-Correlator: 22, 34 -- References: {200905281322.n4SDMjxZ028879-at-ns.microscopy.com} 22, 34 -- X-NAIMIME-Disclaimer: 1 22, 34 -- X-NAIMIME-Modified: 1 22, 34 -- X-NAI-Spam-Score: 0 22, 34 -- X-NAI-Spam-Rules: 1 Rules triggered 22, 34 -- RV3284=0 22, 34 -- Content-Transfer-Encoding: 8bit 22, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SDOi19032193 ==============================End of - Headers==============================
One of the early TEM was inverted. The column ran at an angle from the floor so the operator could look down at the screen. Rumor has it, the rocket ship control pannels in a Flash Gorden serial from the 30's starring Buster Crabbe from the -30's used a non-working inverted TEM. I want to guess it was a Semens, but it just a guess.
DusevichV-at-umkc.ed u To 05/28/2009 09:08 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] RE: viaWWW: SEM and DusevichV-at-umkc.ed TEM Column Orientation u
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
For SEM it would be very inconvenient to mount specimens upside down, in addition all the dirt and dust will fall down in the direction of an electron gun, contaminating it. TEM column is too tall to mount it upside down, and fluorescence screen should be transparent. Horizontal positioning will take much more space than vertical one, and again will introduce inconvenience in mounting SEM specimens and observation of TEM screen.
Physics allows any positioning of a column in space; ergonomics speaks for traditional one, vertical with a gun on the top.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } Sent: Thursday, May 28, 2009 7:40 AM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } This Question/Comment was submitted to the Microscopy } Listserver using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } -------------------------------------------------------------- } ------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both liina170-at-hot.ee as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } ------------- } } Email: liina170-at-hot.ee } Name: Liina } } Organization: University of Tartu } } Title-Subject: [Filtered] SEM and TEM } } Question: What would be the main advantages and disadvantages } of a SEM that operates upside down i.e. has e-gun at the } bottom pointing upwards. What about horizontal design? The } same questions about TEM. } } } Login Host: 213.184.38.91 } -------------------------------------------------------------- } ------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n4SCcqfd009244 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } 2009 07:38:53 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM } and TEM Column Orientation 7, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu [134.193.143.167]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SCwsIq007428 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 07:58:55 -0500 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="us-ascii" 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 8, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) FILETIME= [0D6DBFE0:01C9DF94] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SCwsIq007428 ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 22 -- From frank_karl-at-lincolnelectric.com Thu May 28 08:30:07 2009 25, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 25, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDU5an013659 25, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:30:06 -0500 25, 22 -- In-Reply-To: {200905281308.n4SD8gpL020571-at-ns.microscopy.com} 25, 22 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation 25, 22 -- To: DusevichV-at-umkc.edu, Microscopy-at-microscopy.com 25, 22 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 25, 22 -- Message-ID: {OF047B30F9.825D96CF-ON852575C4.00499E1E-852575C4.004A25E8-at-lincolnelectric.com} 25, 22 -- Date: Thu, 28 May 2009 09:30:00 -0400 25, 22 -- From: Frank_Karl-at-lincolnelectric.com 25, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 25, 22 -- 07, 2008) at 05/28/2009 09:29:53 AM, 25, 22 -- CD-MIME complete at 05/28/2009 09:29:54 AM, 25, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 25, 22 -- 07, 2008) at 05/28/2009 09:29:54 AM, 25, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 25, 22 -- 07, 2008) at 05/28/2009 09:29:54 AM, 25, 22 -- Serialize complete at 05/28/2009 09:29:54 AM 25, 22 -- MIME-Version: 1.0 25, 22 -- Content-Type: text/plain; 25, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
Yes. It makes just everything more complicated but INTRINSICALLY it is feasible! You can also look at your samples with your head down and your feet up. It is possible and does not change the results, it is just not convenient! It is not advised to lay down horizontally along the table during the observations neither :-) (but it is possible)
Stéphane
----- Original Message ---- X-from: "colijn.1-at-osu.edu" {colijn.1-at-osu.edu} To: nizets2-at-yahoo.com Sent: Thursday, May 28, 2009 3:23:12 PM
Not to mention that on an inverted column the electrons have to overcome gravity to make their way from the gun to the sample! {...grin...}
Henk
At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:
} For SEM it would be very inconvenient to mount specimens upside down, in } addition all the dirt and dust will fall down in the direction of an } electron gun, contaminating it. TEM column is too tall to mount it } upside down, and fluorescence screen should be transparent. Horizontal } positioning will take much more space than vertical one, and again will } introduce inconvenience in mounting SEM specimens and observation of TEM } screen. } } Physics allows any positioning of a column in space; ergonomics speaks } for traditional one, vertical with a gun on the top. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } } -----Original Message----- } } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } } Sent: Thursday, May 28, 2009 7:40 AM } } To: Dusevich, Vladimir } } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } } } } } } -------------------------------------------------------------- } } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------- } } -------------- } } } } This Question/Comment was submitted to the Microscopy } } Listserver using the WWW based Form at } } http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------- } } ------------- } } Remember this posting is most likely not from a Subscriber, } } so when replying } } please copy both liina170-at-hot.ee as well as the } } MIcroscopy Listserver } } -------------------------------------------------------------- } } ------------- } } } } Email: liina170-at-hot.ee } } Name: Liina } } } } Organization: University of Tartu } } } } Title-Subject: [Filtered] SEM and TEM } } } } Question: What would be the main advantages and disadvantages } } of a SEM that operates upside down i.e. has e-gun at the } } bottom pointing upwards. What about horizontal design? The } } same questions about TEM. } } } } } } Login Host: 213.184.38.91 } } -------------------------------------------------------------- } } ------------- } } } } ==============================Original } } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 } } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n4SCcqfd009244 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } } 2009 07:38:53 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by } } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM } } and TEM Column Orientation 7, 11 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 } 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu } (smtp.exchange.umkc.edu [134.193.143.167]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4SCwsIq007428 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 } 07:58:55 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by } kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 } 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation } 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 } 8, 25 -- Message-ID: } {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation } 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA } 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) } FILETIME=[0D6DBFE0:01C9DF94] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4SCwsIq007428 } ==============================End of - Headers==============================
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 7, 26 -- From colijn.1-at-osu.edu Thu May 28 08:19:02 2009 7, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDJ0tP023075 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:19:01 -0500 7, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 7, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 7, 26 -- id {01N9HBFG6Y8W8XEJHW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 7, 26 -- Thu, 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Received: from HOC1.ecr6.ohio-state.edu 7, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 7, 26 -- (PMDF V6.3-x18 #31556) 7, 26 -- with ESMTPA id {01N9HBFFWDQQ8X5P9B-at-ecr6.ohio-state.edu} ; Thu, 7, 26 -- 28 May 2009 09:18:58 -0400 (EDT) 7, 26 -- Date: Thu, 28 May 2009 09:20:24 -0400 7, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 26 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation 7, 26 -- In-reply-to: {200905281300.n4SD0xOu009804-at-ns.microscopy.com} 7, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 26 -- To: DusevichV-at-umkc.edu 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 -- Message-id: {01N9HBFFXPS48X5P9B-at-ecr6.ohio-state.edu} 7, 26 -- MIME-version: 1.0 7, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 7, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 7, 26 -- References: {200905281300.n4SD0xOu009804-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 22, 24 -- From nizets2-at-yahoo.com Thu May 28 08:32:23 2009 22, 24 -- Received: from web110808.mail.gq1.yahoo.com (web110808.mail.gq1.yahoo.com [67.195.13.231]) 22, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4SDWKcd019506 22, 24 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:32:22 -0500 22, 24 -- Received: (qmail 34028 invoked by uid 60001); 28 May 2009 13:32:17 -0000 22, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1243517537; bh=GELY6Y7CKH6CK3+B/0tEu0axgMhFfL3i12mNqfwGKNk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=QjuiI9n+d6CwKVl3teLmUH8vSydt43loICS5kJupmtwMVPIqie170sRMOneXjX+fQF473GI5lge6Rfuiy0PLDO7qWBqH96TzCtXXyJGHTen5OQuPgCI0d5lqcyJ9tvAth7EeyBt1JEiquD/w7hB9y3C0/5p2qBE1nQnBKVCXK7g= 22, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 22, 24 -- s=s1024; d=yahoo.com; 22, 24 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 22, 24 -- b=dog4PKHgBzqsQQ17vMQPddhrLKf5DnIpWRJ6BUSCnZlRkjOyyv0ErPnkCLtKmAvwwajnteFpv2BrJ47HV8+GwLT/Cp5MOIGwY8tDGXnnj8Q52XCZhgs/rn5gTJxixYrdY58/RAT4cMUUvn9IfrO5w9qb59rXfqIPavjc71QaAyg=; 22, 24 -- Message-ID: {615381.33741.qm-at-web110808.mail.gq1.yahoo.com} 22, 24 -- X-YMail-OSG: Piw6cBYVM1ljMjYx7xGtj4nUDb018uoslRQOr6pcZq1cjIUgnB0wUbNckFr8fi6tJm4bnLMQJb48EFYq4fCEh6ga3a636XqnpQEzmGyVhFvCckug6oGNPldthlAbNKabGDA0yom37AuHJ3DRgqgv7W.MTi1x0jwnNEoCJSFcqR9OjtK9KGqIz7fhPoCzHV2qyYLFnl_f4_dUQVcB491UBUEJymXHqQIRc2NfY05ZDqv.4..LMQQTEesdAVY_30zo.Qfjlt18D.Phjt3ifOmdO4NtEdH2.RfNxdDeB.J.LWFUKHIWNP9f4TNWA6IB1A6m1ASZrVRS7mooXT0oDf._2I41K14W9d9YtkGCdkkKkw-- 22, 24 -- Received: from [80.122.101.100] by web110808.mail.gq1.yahoo.com via HTTP; Thu, 28 May 2009 06:32:17 PDT 22, 24 -- X-Mailer: YahooMailRC/1277.43 YahooMailWebService/0.7.289.10 22, 24 -- References: {200905281323.n4SDNCPn029656-at-ns.microscopy.com} 22, 24 -- Date: Thu, 28 May 2009 06:32:17 -0700 (PDT) 22, 24 -- From: Stephane Nizet {nizets2-at-yahoo.com} 22, 24 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation 22, 24 -- To: microscopy-at-microscopy.com 22, 24 -- In-Reply-To: {200905281323.n4SDNCPn029656-at-ns.microscopy.com} 22, 24 -- MIME-Version: 1.0 22, 24 -- Content-Type: text/plain; charset=iso-8859-1 22, 24 -- Content-Transfer-Encoding: 8bit 22, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SDWKcd019506 ==============================End of - Headers==============================
This reminded me of a conversation I overheard between a couple of Professors about how electrons behave in a gravitational field. The Theoretician pointed out that, as a source of energy, gravity is extremely coherent and would be a good for interferometry. The Experimentalist pointed out that the electron column would have to be several hundred light years long for TEM-like energies. Just don't ask how one would align the gun of a microscope that long!
} } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Not to mention that on an inverted column the electrons have to overcome } gravity to make their way from the gun to the sample! {...grin...} } } Henk
==============================Original Headers============================== 4, 27 -- From jsb43-at-hermes.cam.ac.uk Thu May 28 08:34:30 2009 4, 27 -- Received: from ppsw-6.csi.cam.ac.uk (ppsw-6.csi.cam.ac.uk [131.111.8.136]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SDYT50025885 4, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 08:34:30 -0500 4, 27 -- X-Cam-AntiVirus: no malware found 4, 27 -- X-Cam-SpamDetails: not scanned 4, 27 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 4, 27 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:45447) 4, 27 -- by ppsw-6.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25) 4, 27 -- with esmtpa (EXTERNAL:jsb43) id 1M9fkU-0006Ba-JN (Exim 4.70) for Microscopy-at-microscopy.com 4, 27 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 28 May 2009 14:34:22 +0100 4, 27 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk) 4, 27 -- with local (PRAYER:jsb43) id 1M9fkT-0005fk-W0 (Exim 4.67) for Microscopy-at-microscopy.com 4, 27 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 28 May 2009 14:34:21 +0100 4, 27 -- Received: from [131.111.102.18] by webmail.hermes.cam.ac.uk 4, 27 -- with HTTP (Prayer-1.3.1); 28 May 2009 14:34:21 +0100 4, 27 -- Date: 28 May 2009 14:34:21 +0100 4, 27 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 4, 27 -- To: MSA Listserver {Microscopy-at-microscopy.com} 4, 27 -- Subject: Re: SEM and TEM Column Orientation 4, 27 -- Message-ID: {Prayer.1.3.1.0905281434210.16334-at-hermes-2.csi.cam.ac.uk} 4, 27 -- In-Reply-To: {200905281323.n4SDNghq030392-at-ns.microscopy.com} 4, 27 -- References: {200905281323.n4SDNghq030392-at-ns.microscopy.com} 4, 27 -- X-Mailer: Prayer v1.3.1 4, 27 -- Mime-Version: 1.0 4, 27 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 4, 27 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
I have a vintage brochure for the Corinth 500, which was an upside-down TEM. You straddled the column, and the specimen exchange port was right at crotch level! They must have had GREAT faith in their x-ray shielding! A scan of the cover is at:
http://www.mta.ca/dmf/download/jme/corinth500.htm
Enjoy!
JME -- - Does the name Pavlov ring a bell? --
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
CamScan (UK) make the X500 crystal probe (http://www.camscan.com/X500.htm) - optimised for EBSD analysis with the column tilted through 70°. The original concept was to allow rocks to be heated to close to their melting point and to avoid them dripping (or spalling) off the horizontal stage.
Visitec (Germany, http://www.visitec-em.de/ ) make Mira - an SEM with a chamber large enough to stand inside and a column that moves around and tilts (http://www.visitec-em.de/cms/en/?Products:Positioning_system). Mira can look at very large specimens, e.g. satellites, car engines (there's even a photo of the head of Terracotta warrior).
The main advantage is that unusual specimens & geometries can be used.
Hope this helps,
Austin
----- Original Message ----- X-from: {liina170-at-hot.ee} To: {AuntDaisy-at-gmail.com} Sent: Thursday, May 28, 2009 1:45 PM
Our laboratory purchased an AEI Corinth 275 TEM with an inverted column in 1973 (before my time!) The director reported it was a beast to align, manually, the seven steel rings that comprised the column; and there were a series of frustrating problems beginning at installation and continuing for three long years. Apparently, though, the optics were quite good (7 Angstrom resolution), and the wood cabinet was beautiful. The microscope remained in operation until 1981. There's more information and a picture in the history section on our website, if you are interested: http://www.ndsu.nodak.edu/ndsu/em_lab/documents/History.pdf
Jayma Moore, DVM MS Laboratory Manager Electron Microscopy Center North Dakota State University Fargo
==============================Original Headers============================== 3, 19 -- From jayma.moore-at-ndsu.edu Thu May 28 09:11:47 2009 3, 19 -- Received: from smtp1.NoDak.edu (smtp1.NoDak.edu [134.129.111.50]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SEBkHt016364 3, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 May 2009 09:11:47 -0500 3, 19 -- Received: from [134.129.124.162] (dyn162.124.ndsu.NoDak.edu [134.129.124.162]) 3, 19 -- (authenticated bits=0) 3, 19 -- by smtp1.NoDak.edu (8.13.1/8.13.1) with ESMTP id n4SEBk2F010229 3, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 3, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 May 2009 09:11:46 -0500 3, 19 -- Message-ID: {4A1E9B9E.8040900-at-ndsu.edu} 3, 19 -- Date: Thu, 28 May 2009 09:11:42 -0500 3, 19 -- From: "Jayma A. Moore DVM" {jayma.moore-at-ndsu.edu} 3, 19 -- Organization: NDSU Electron Microscopy Center 3, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 3, 19 -- MIME-Version: 1.0 3, 19 -- To: Microscopy-at-Microscopy.Com 3, 19 -- Subject: Inverted TEM 3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Actually there have been a number of horizontal/inclined and inverted column microscopes. Some that I can come up with off the top of my head are:
All of the VG STEMs (inverted) Nion STEMs (inverted) Philips EM100 (inclined) JEOL SuperScope (inclined, JEM-50?, 50kV?) AEI Corinth (inverted) Vickers EM4 (early 1950s?) Shimadzu SM-C2 (horizontal, early 1950s?)
Can anyone else come up with any? How about photos?
Cheers, Henk
At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:
} For SEM it would be very inconvenient to mount specimens upside down, in } addition all the dirt and dust will fall down in the direction of an } electron gun, contaminating it. TEM column is too tall to mount it } upside down, and fluorescence screen should be transparent. Horizontal } positioning will take much more space than vertical one, and again will } introduce inconvenience in mounting SEM specimens and observation of TEM } screen. } } Physics allows any positioning of a column in space; ergonomics speaks } for traditional one, vertical with a gun on the top. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } } -----Original Message----- } } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } } Sent: Thursday, May 28, 2009 7:40 AM } } To: Dusevich, Vladimir } } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } } } } } } -------------------------------------------------------------- } } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------- } } -------------- } } } } This Question/Comment was submitted to the Microscopy } } Listserver using the WWW based Form at } } http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------- } } ------------- } } Remember this posting is most likely not from a Subscriber, } } so when replying } } please copy both liina170-at-hot.ee as well as the } } MIcroscopy Listserver } } -------------------------------------------------------------- } } ------------- } } } } Email: liina170-at-hot.ee } } Name: Liina } } } } Organization: University of Tartu } } } } Title-Subject: [Filtered] SEM and TEM } } } } Question: What would be the main advantages and disadvantages } } of a SEM that operates upside down i.e. has e-gun at the } } bottom pointing upwards. What about horizontal design? The } } same questions about TEM. } } } } } } Login Host: 213.184.38.91 } } -------------------------------------------------------------- } } ------------- } } } } ==============================Original } } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 } } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n4SCcqfd009244 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } } 2009 07:38:53 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by } } way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM } } and TEM Column Orientation 7, 11 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 } 8, 25 -- Received: from kc-msxproto1.kc.umkc.edu } (smtp.exchange.umkc.edu [134.193.143.167]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4SCwsIq007428 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 } 07:58:55 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by } kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 } 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 8, 25 -- Content-class: urn:content-classes:message } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation } 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 } 8, 25 -- Message-ID: } {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation } 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA } 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 8, 25 -- To: {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} } 8, 25 -- X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) } FILETIME=[0D6DBFE0:01C9DF94] } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4SCwsIq007428 } ==============================End of - Headers==============================
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 10, 22 -- From colijn.1-at-osu.edu Thu May 28 09:18:15 2009 10, 22 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SEIFud005161 10, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:18:15 -0500 10, 22 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 22 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 10, 22 -- id {01N9HDHXTPQO8XEUCW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 10, 22 -- Thu, 28 May 2009 10:18:14 -0400 (EDT) 10, 22 -- Received: from HOC1.ecr6.ohio-state.edu 10, 22 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 10, 22 -- (PMDF V6.3-x18 #31556) with ESMTPA id {01N9HDHWLVVY8XKOCN-at-ecr6.ohio-state.edu} 10, 22 -- for Microscopy-at-microscopy.com; Thu, 28 May 2009 10:18:13 -0400 (EDT) 10, 22 -- Date: Thu, 28 May 2009 10:19:40 -0400 10, 22 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 22 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation 10, 22 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 22 -- To: Microscopy-at-microscopy.com 10, 22 -- Message-id: {01N9HDHWOAIO8XKOCN-at-ecr6.ohio-state.edu} 10, 22 -- MIME-version: 1.0 10, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 10, 22 -- X-Env-From: auth/colijn.1-at-osu.edu ==============================End of - Headers==============================
Hello Everyone, I was asked to forward this to the list and thought, "why not?"
Stay safe
"cannonmp" {cannonmp-at-comcast .net} To {Frank_Karl-at-lincolnelectric.com} 05/28/2009 10:28 cc AM Subject Re: [Microscopy] viaWWW: SEM and TEM Column Orientation
Hello Frank,
For some reason I can't post to the list even though I was a very early member.
I believe that the RCA EM-100 may have been the first commercial TEM. Its column was oriented at approximately 45 degrees to the floor. The screen was viewed directly like a television set. The cabinet was a cast and polished console which did indeed look like something out of a 1940s space movie.
I bought one of these at a surplus sale in 1984, and like a pure idiot I scrapped the console. It was GORGEOUS and I regret that decision to this day.
Think of what a fantastic cocktail bar that console would have made.
Forward to the list if you can.
Bart Cannon Cannon Microprobe Seattle ----- Original Message ----- X-from: {Frank_Karl-at-lincolnelectric.com} To: {cannonmp-at-comcast.net} Sent: Thursday, May 28, 2009 6:39 AM
Hello, there is a quite recent device LVEM5 produced by Delong Instruments. It is a low voltage electron microscope and it is "true inverted". Best regards from Prague Oldrich
P.S. I'm not sure if I may post here a WEBlink to DI company. They have there a nice image of it and a lot of info.
On Thursday 28 of May 2009 16:20:37 colijn.1-at-osu.edu wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --------------------------------------------------------------------------- } - } } Actually there have been a number of horizontal/inclined and inverted } column microscopes. Some that I can come up with off the top of my head } are: } } All of the VG STEMs (inverted) } Nion STEMs (inverted) } Philips EM100 (inclined) } JEOL SuperScope (inclined, JEM-50?, 50kV?) } AEI Corinth (inverted) } Vickers EM4 (early 1950s?) } Shimadzu SM-C2 (horizontal, early 1950s?) } } Can anyone else come up with any? How about photos? } } Cheers, } Henk
==============================Original Headers============================== 5, 23 -- From benada-at-biomed.cas.cz Thu May 28 10:05:17 2009 5, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SF5HEX004591 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 10:05:17 -0500 5, 23 -- Received: from ob117nb.localnet (u117ob.mbu.cas.cz [147.231.44.101]) 5, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 23 -- (No client certificate requested) 5, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id A51A21A4452B 5, 23 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 17:05:16 +0200 (CEST) 5, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 5, 23 -- Organization: Institute of Microbiology 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation 5, 23 -- Date: Thu, 28 May 2009 17:04:41 +0200 5, 23 -- User-Agent: KMail/1.11.2 (Linux/2.6.29-2-686; KDE/4.2.2; i686; ; ) 5, 23 -- References: {200905281420.n4SEKbwS010884-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {200905281420.n4SEKbwS010884-at-ns.microscopy.com} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: Text/Plain; 5, 23 -- charset="utf-8" 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- Content-Disposition: inline 5, 23 -- Message-Id: {200905281704.41764.benada-at-biomed.cas.cz} ==============================End of - Headers==============================
On May 28, 2009, at 5:39 AM, liina170-at-hot.ee wrote:
} What would be the main advantages and disadvantages of a } SEM that operates upside down i.e. has e-gun at the bottom pointing } upwards. What about horizontal design? The same questions about TEM.
Dear Liina, A big disadvantage that no one has yet mentioned for TEM is that brehmsstrahlung x-rays are forward directed and can have the same energy as the beam electrons. Since TEMs are usually placed in rooms that are on the lowest floor, the conventional orientation causes most of the radiation to be directed harmlessly into the earth; whereas, an inverted orientation would direct them into the floor above. For lower voltage instruments, the intervening ceiling and floor might be enough to absorb the radiation, but for higher voltage instruments, there could be an unsafe level of radiation that makes it into the room above the scope. For the HVEM in Albany, NY, we were very concerned about radiation scattering in various directions, since a 1.2 MeV photon will traverse a few meters of lead. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu May 28 11:24:33 2009 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SGOW59023112 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 11:24:33 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 8170766E471F 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:32 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 8F2E566E4720 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:31 -0700 (PDT) 6, 22 -- Message-Id: {5346CC5F-3A8C-42FB-B929-914A48421F67-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200905281239.n4SCd1ai009416-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3) 6, 22 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation 6, 22 -- Date: Thu, 28 May 2009 09:24:31 -0700 6, 22 -- References: {200905281239.n4SCd1ai009416-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.935.3) ==============================End of - Headers==============================
Years ago ( won't say how many as that might give away my age...and I try to think young!!) I worked in the laboratory of Humberto Fernandez-Moran at the U of Chicago. Moran had a large scar on his nose where he had a cancerous lesion removed. He swore that it was from pressing his nose against the window of a TEM before TEM windows were lead-coated to prevent release of radiation.
This response just triggered the memory..... -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: Bill Tivol {tivol-at-caltech.edu} } Reply-To: Bill Tivol {tivol-at-caltech.edu} } Date: Thu, 28 May 2009 11:26:42 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On May 28, 2009, at 5:39 AM, liina170-at-hot.ee wrote: } } } What would be the main advantages and disadvantages of a } } SEM that operates upside down i.e. has e-gun at the bottom pointing } } upwards. What about horizontal design? The same questions about TEM. } } } Dear Liina, } A big disadvantage that no one has yet mentioned for TEM is that } brehmsstrahlung x-rays are forward directed and can have the same } energy as the beam electrons. Since TEMs are usually placed in rooms } that are on the lowest floor, the conventional orientation causes most } of the radiation to be directed harmlessly into the earth; whereas, an } inverted orientation would direct them into the floor above. For } lower voltage instruments, the intervening ceiling and floor might be } enough to absorb the radiation, but for higher voltage instruments, } there could be an unsafe level of radiation that makes it into the } room above the scope. For the HVEM in Albany, NY, we were very } concerned about radiation scattering in various directions, since a } 1.2 MeV photon will traverse a few meters of lead. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 6, 22 -- From tivol-at-caltech.edu Thu May 28 11:24:33 2009 } 6, 22 -- Received: from outgoing-mail.its.caltech.edu } (outgoing-mail.its.caltech.edu [131.215.239.19]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } n4SGOW59023112 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 11:24:33 -0500 } 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) } 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 8170766E471F } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:32 -0700 } (PDT) } 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new } 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu } [131.215.19.146]) } 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 8F2E566E4720 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:24:31 -0700 } (PDT) } 6, 22 -- Message-Id: {5346CC5F-3A8C-42FB-B929-914A48421F67-at-caltech.edu} } 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- In-Reply-To: {200905281239.n4SCd1ai009416-at-ns.microscopy.com} } 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 6, 22 -- Content-Transfer-Encoding: 7bit } 6, 22 -- Mime-Version: 1.0 (Apple Message framework v935.3) } 6, 22 -- Subject: Re: [Microscopy] viaWWW: SEM and TEM Column Orientation } 6, 22 -- Date: Thu, 28 May 2009 09:24:31 -0700 } 6, 22 -- References: {200905281239.n4SCd1ai009416-at-ns.microscopy.com} } 6, 22 -- X-Mailer: Apple Mail (2.935.3) } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 31 -- From dsherman-at-purdue.edu Thu May 28 11:33:55 2009 5, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SGXto5004504 5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 11:33:55 -0500 5, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 5, 31 -- by mailhub130.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id n4SGXsiK018742 5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 12:33:54 -0400 5, 31 -- Received: from 1061exfe03a.purdue.lcl (1061exfe03a.itap.purdue.edu [128.210.1.10]) 5, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id n4SGXsQk012678 5, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 12:33:54 -0400 5, 31 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe03a.purdue.lcl with Microsoft SMTPSVC(6.0.3790.3959); 5, 31 -- Thu, 28 May 2009 12:33:54 -0400 5, 31 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 5, 31 -- Thu, 28 May 2009 16:33:07 +0000 5, 31 -- User-Agent: Microsoft-Entourage/12.15.0.081119 5, 31 -- Date: Thu, 28 May 2009 12:33:06 -0400 5, 31 -- Subject: Re: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation 5, 31 -- From: Debby Sherman {dsherman-at-purdue.edu} 5, 31 -- To: Bill Tivol {tivol-at-caltech.edu} , 5, 31 -- "message to: MSA list" {microscopy-at-microscopy.com} 5, 31 -- Message-ID: {C6443502.3D7B7%dsherman-at-exchange.purdue.edu} 5, 31 -- Thread-Topic: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation 5, 31 -- Thread-Index: Acnfsfpmth/eH0j0RW2EstDwM8XAXw== 5, 31 -- In-Reply-To: {200905281626.n4SGQgBv026261-at-ns.microscopy.com} 5, 31 -- Mime-version: 1.0 5, 31 -- Content-type: text/plain; 5, 31 -- charset="US-ASCII" 5, 31 -- Content-transfer-encoding: 7bit 5, 31 -- X-OriginalArrivalTime: 28 May 2009 16:33:54.0477 (UTC) FILETIME=[174B8DD0:01C9DFB2] 5, 31 -- X-PMX-Version: 5.4.0.320885 5, 31 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
I picked up this thread a bit late, so please excuse me for any duplication. I cut my eyeteeth, as it were, on a Phillips EM100, which had a horizontal column with a very thick viewing screen right up against my face. The instrument looked like a front loading washing machine In order to align the column, you had to mount a mirror on the wall so that you could get behind the instrument and see the images on the screen. A few years ago, I came across one of these in the Science Museum in Delft. How strange to see something I worked with, and published images from, sitting stripped down as an historical artifact.
No evidence of any problems yet (45 years ago), but I always wondered about radiation exposure. -- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 3, 39 -- From joelsheffield-at-gmail.com Thu May 28 12:04:54 2009 3, 39 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.144]) 3, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SH4sJi020284 3, 39 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 12:04:54 -0500 3, 39 -- Received: by qw-out-1920.google.com with SMTP id 4so1658796qwk.54 3, 39 -- for {microscopy-at-microscopy.com} ; Thu, 28 May 2009 10:04:53 -0700 (PDT) 3, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 39 -- d=gmail.com; s=gamma; 3, 39 -- h=domainkey-signature:received:received:from:to:date:mime-version 3, 39 -- :subject:message-id:priority:x-mailer:content-type 3, 39 -- :content-transfer-encoding:content-description; 3, 39 -- bh=AWWPTd2YI+PBNE4RiNUbWDC/BKXcl1/d6XsWpvBy6LM=; 3, 39 -- b=CyYX88H9dBgSlZSFLtRqVlQEzeRZCc09/hRUHiNH2YfdMwV+jnUKi2pZOPcyPC6r8k 3, 39 -- KSZbE1B0t9mGyqO7pz5dypsrnxl1TJEM7Z6lUT8hnhIgfqrbx8dkYQ9qJBHs8tbLebID 3, 39 -- nl1KDgLQ27sHB4+dtLfsYZbsfgqBsHkKiSeu0= 3, 39 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 39 -- d=gmail.com; s=gamma; 3, 39 -- h=from:to:date:mime-version:subject:message-id:priority:x-mailer 3, 39 -- :content-type:content-transfer-encoding:content-description; 3, 39 -- b=osiLyqiRjsGzI0FTweQ0Us1/9kLZQFKaiG3wENYyhQdVATwoj2JEAH6od9R82jcG/k 3, 39 -- fuheJqFa9D3pvnbRO4Jma193GU0HfOG94YkvBuFjh+dBdzU/qCPEuCFvPzt0eTOm97pz 3, 39 -- rZJ2m9YGfExJZoS3JPQleW4AkwFbZeAwksHnk= 3, 39 -- Received: by 10.229.80.137 with SMTP id t9mr814480qck.68.1243530293049; 3, 39 -- Thu, 28 May 2009 10:04:53 -0700 (PDT) 3, 39 -- Received: from ?155.247.98.40? (jbs.bio.temple.edu [155.247.98.40]) 3, 39 -- by mx.google.com with ESMTPS id 4sm684062yxq.44.2009.05.28.10.04.52 3, 39 -- (version=SSLv3 cipher=RC4-MD5); 3, 39 -- Thu, 28 May 2009 10:04:52 -0700 (PDT) 3, 39 -- From: joelsheffield-at-gmail.com 3, 39 -- To: microscopy-at-microscopy.com 3, 39 -- Date: Thu, 28 May 2009 13:05:18 -0400 3, 39 -- MIME-Version: 1.0 3, 39 -- Subject: Re: [Microscopy] Re: viaWWW: SEM and TEM Column Orientation 3, 39 -- Message-ID: {4A1E8C0E.10390.4367B3F-at-joelsheffield.gmail.com} 3, 39 -- Priority: normal 3, 39 -- X-mailer: Pegasus Mail for Windows (4.41) 3, 39 -- Content-type: text/plain; charset=US-ASCII 3, 39 -- Content-transfer-encoding: 7BIT 3, 39 -- Content-description: Mail message body ==============================End of - Headers==============================
After reading about this for a few months, I decided to actually call our Occupational Safety department for some facts. Although we are all scientists, this is not our actual job training. Our representative DID NOT FIND ANY recorded deaths from single compressed nitrogen or carbon dioxide gas tank failure. (If you know of any, feel free to share.)
Our microscopes are all in lab-rated rooms. The ventilation system refreshes the air 6-10 times per hour by law (depending on the exact room settings). The largest compressed nitrogen tank (L1) holds 300 cubic feet of air. Our confocal microscope room is 8x16x8 feet in dimensions; which equals 1,024 cubic feet. If that tank suffers catastrophic failure, the user goes from 20.9% oxygen to 14.8% oxygen. The gas will mix with the room air quickly, and the ratio will return to 20% in 10 minutes or less.
LIQUID NITROGEN HAS killed people, and here's the difference: One liter of liquid nitrogen expands to 696 liters of nitrogen gas! In addition, that nitrogen will mix with the room air more slowly due to the cooler temperature of the nitrogen.
Our unoccupied rooms are still rated at a total air replacement rate of 4 times per hour. Even in a small, airtight room the conditions for suffocation are low. Anything's possible, but I think we can leave the issue of dry nitrogen gas versus mixed gas up to the individual purchaser. Safety is not the overriding factor.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Wednesday, May 27, 2009 5:00 AM To: Sobocinski, Gregg
Dale
I'm sorry but there are too many "mosts" and "probablys" in your argument and to even say that oxygen content is approaching death zone levels makes the basic assumption that any victim is a fit acclimatized mountain climber to be relatively safe.
Risk assessment must look at the worst scenario anyway and assume that someone may enter the room after the noisy leakage event occurred. They would have no warning and would almost certainly be unaware of what was happening to them. They might be the cleaner or an inexperienced student and be in the room alone before/after anybody else is in the building.
People do die in workplace/lab situations from oxygen depletion and there is always the risk that rescue attempts can put more people at risk.
So I would say do the risk assessment for your workplace looking at the worst leakage and find out what would be considered a safe and acceptable level of oxygen (certainly not as low as 12%). If your lab falls below those requirements then do something such as better ventilation, smaller source of gas or an oxygen depletion monitor with an alarm.
Cheers
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: dac-at-research.umass.edu
==============================Original Headers============================== 12, 27 -- From malcolm.haswell-at-sunderland.ac.uk Wed May 27 03:49:32 2009 12, 27 -- Received: from max2.sunderland.ac.uk (max2.sunderland.ac.uk [157.228.98.76]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4R8nVeO018508 12, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 May 2009 03:49:31 -0500 12, 27 -- Received: (qmail 1804 invoked from network); 27 May 2009 08:44:26 -0000 12, 27 -- Received: from localhost (127.0.0.1) 12, 27 -- by max2.sunderland.ac.uk with SMTP; 27 May 2009 08:44:26 -0000 12, 27 -- Received: (qmail 1742 invoked by uid 599); 27 May 2009 08:44:20 -0000 12, 27 -- Received: from unknown (HELO hermes.sunderland.ac.uk) (157.228.37.117) 12, 27 -- by max2.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Wed, 27 May 2009 09:44:20 +0100 12, 27 -- Received: from [157.228.164.221] by hermes.sunderland.ac.uk (mshttpd); Wed, 12, 27 -- 27 May 2009 09:44:21 +0100 12, 27 -- Date: Wed, 27 May 2009 09:44:21 +0100 12, 27 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} 12, 27 -- Subject: Re: [Microscopy] Re: viaWWW: Nitrogen leak (is this really a problem?) 12, 27 -- To: Microscopy MSA {Microscopy-at-microscopy.com} 12, 27 -- Cc: dac-at-research.umass.edu 12, 27 -- Message-id: {241418243950.243950241418-at-sunderland.ac.uk} 12, 27 -- MIME-version: 1.0 12, 27 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004) 12, 27 -- Content-type: text/plain; charset=us-ascii 12, 27 -- Content-language: en 12, 27 -- Content-transfer-encoding: 7BIT 12, 27 -- Content-disposition: inline 12, 27 -- X-Accept-Language: en 12, 27 -- Priority: normal 12, 27 -- X-Virus-Scanned: by iCritical at max2.sunderland.ac.uk ==============================End of - Headers==============================
==============================Original Headers============================== 22, 31 -- From greggps-at-umich.edu Thu May 28 13:10:14 2009 22, 31 -- Received: from itcs-ehub-02.adsroot.itcs.umich.edu (itcs-ehub-02.adsroot.itcs.umich.edu [141.211.3.202]) 22, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SIADRa004499 22, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 13:10:14 -0500 22, 31 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.234]) by 22, 31 -- itcs-ehub-02.adsroot.itcs.umich.edu ([141.211.3.202]) with mapi; Thu, 28 May 22, 31 -- 2009 14:10:12 -0400 22, 31 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 22, 31 -- To: Microscopy MSA {Microscopy-at-microscopy.com} 22, 31 -- Date: Thu, 28 May 2009 14:10:08 -0400 22, 31 -- Subject: Nitrogen gas leaks are NOT lethal in a lab 22, 31 -- Thread-Topic: Nitrogen gas leaks are NOT lethal in a lab 22, 31 -- Thread-Index: AcneqXqJF7NIcxAuTnWAj+cXcyHw+QAKCbXA 22, 31 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA39340611A6154F-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} 22, 31 -- References: {200905270859.n4R8xfuk030739-at-ns.microscopy.com} 22, 31 -- In-Reply-To: {200905270859.n4R8xfuk030739-at-ns.microscopy.com} 22, 31 -- Accept-Language: en-US 22, 31 -- Content-Language: en-US 22, 31 -- X-MS-Has-Attach: 22, 31 -- X-MS-TNEF-Correlator: 22, 31 -- x-cr-puzzleid: {89223E7D-1660-4E8D-83E4-582F72E82DA7} 22, 31 -- x-cr-hashedpuzzle: Amm/ ChDg Colp CyUw DIbN DW9u EHGq EJ/H Eki1 GL03 G5Bh 22, 31 -- HsSE IBXc IKxe IP1O 22, 31 -- IeOS;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{89223E7D-1660-4E8D-83E4-582F72E82DA7};ZwByAGUAZwBnAHAAcwBAAHUAbQBpAGMAaAAuAGUAZAB1AA==;Thu, 22, 31 -- 28 May 2009 18:10:08 22, 31 -- GMT;TgBpAHQAcgBvAGcAZQBuACAAZwBhAHMAIABsAGUAYQBrAHMAIABhAHIAZQAgAE4ATwBUACAAbABlAHQAaABhAGwAIABpAG4AIABhACAAbABhAGIA 22, 31 -- acceptlanguage: en-US 22, 31 -- Content-Type: text/plain; charset="us-ascii" 22, 31 -- MIME-Version: 1.0 22, 31 -- Content-Transfer-Encoding: 8bit 22, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4SIADRa004499 ==============================End of - Headers==============================
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Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] fixation of cAMP for EM
Question: Good morning,
Does anyone have a protocol for fixing cAMP in tissue culture cells? I've heard something about microwave fixation. Thanks in advance.
} } Does anyone have a protocol for fixing cAMP in tissue culture cells? } I've heard something about microwave fixation. Thanks in advance.
Hi Mary,
I am just thinking about three ways: 1. physical, cryofixation, plus freeze-substitution-fixation: I would favor this route. The question is then: does cAMP react with any of the 'chemicals' in the FSF solution, and at which temperature? And: how to detect the product of this further reaction? if you do NOT add any chemical fixative: how to specifically detect or visualize pure cAMP as such? 2+3, chemical, with or without microwave (the first way is worth testing!): 2. chemical, by GA: it appears plausible to me that cAMP can react with aldehydes - whether it really does, may depend on a variety of parameters, though. Is cAMP bound to an enzyme, and is it really freely accessible to the aldehyde? and then, when it has reacted, the cAMP molecule is changed, presumably the base itself. How to specifically detect and visualize? 3. chemical, by OsO4: again, cAMP may react with this chemical. How does it react, and what is the product? how to detect it, selectively? if Os(2) is bound to it, I would guess it is merely a 'black dot', 1 nm in size, in a 120keV machine. In fact, invisible ....
What do others think about this? would be interesting to know.
best regards Reinhard
--
PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 9, 26 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri May 29 01:54:07 2009 9, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4T6s6Z0026176 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 01:54:06 -0500 9, 26 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1]) 9, 26 -- by localhost (Postfix) with SMTP id 0588DABF39 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 08:54:08 +0200 (CEST) 9, 26 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.5.51]) 9, 26 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id E580BABF3B 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 08:54:07 +0200 (CEST) 9, 26 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 9, 26 -- with Novell_GroupWise; Fri, 29 May 2009 08:54:05 +0200 9, 26 -- Message-Id: {4A1FA2A9020000540001543C-at-gwsmtp1.uni-regensburg.de} 9, 26 -- X-Mailer: Novell GroupWise Internet Agent 8.0.0 9, 26 -- Date: Fri, 29 May 2009 08:54:01 +0200 9, 26 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 9, 26 -- To: {mckee-at-helix.mgh.harvard.edu} 9, 26 -- Cc: {microscopy-at-microscopy.com} 9, 26 -- Subject: fixation of cAMP for EM 9, 26 -- References: {200905282245.n4SMjCTY030020-at-ns.microscopy.com} 9, 26 -- In-Reply-To: {200905282245.n4SMjCTY030020-at-ns.microscopy.com} 9, 26 -- Mime-Version: 1.0 9, 26 -- Content-Type: text/plain; charset=US-ASCII 9, 26 -- Content-Disposition: inline 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4T6s6Z0026176 ==============================End of - Headers==============================
Here is a paper in light microscopy (full freely available online).
http://www.jbc.org/cgi/content/full/272/50/31489
Please note the remark about the difficulty to use classical aldehyde fixatives to immobilize cAMP. They also refer to microwave fixation, stating that it has reproducibility issues (which is no reason not to try it :-)). It may be that acrolein is not sufficient to maintain a good overall morphology for EM. In this case I don't see why you could not try to cumulate acrolein (for cAMP) and aldehyde (for ultrastructure) fixation. However one has generally to accept a degradation of the morphology to gain signal by immunodetection. Please also note the possibility to inject conjugated PKA to reveal cAMP. By choosing wisely the conjugate you may be able to use this technique for detection in EM.
Cryo-EM (I mean with cryo-observation, aka CEMOVIS method) is always an option, but it is not widely available.
Regards,
Stephane
----- Original Message ---- X-from: "mckee-at-helix.mgh.harvard.edu" {mckee-at-helix.mgh.harvard.edu} To: nizets2-at-yahoo.com Sent: Friday, May 29, 2009 12:49:24 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckee-at-helix.mgh.harvard.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] fixation of cAMP for EM
Question: Good morning,
Does anyone have a protocol for fixing cAMP in tissue culture cells? I've heard something about microwave fixation. Thanks in advance.
Cambridge Instruments manufactured the Geoscan and the Microscan MKV in the late 60's both of which had horizontal columns. They were SEM's with two large fully focusing WDX.
Regards Derek Simpson
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, May 28, 2009 10:21 AM To: Simpson, Derek
Actually there have been a number of horizontal/inclined and inverted column microscopes. Some that I can come up with off the top of my head are:
All of the VG STEMs (inverted) Nion STEMs (inverted) Philips EM100 (inclined) JEOL SuperScope (inclined, JEM-50?, 50kV?) AEI Corinth (inverted) Vickers EM4 (early 1950s?) Shimadzu SM-C2 (horizontal, early 1950s?)
Can anyone else come up with any? How about photos?
Cheers, Henk
At 09:00 AM 05/28/09, DusevichV-at-umkc.edu wrote:
} For SEM it would be very inconvenient to mount specimens upside down, } in addition all the dirt and dust will fall down in the direction of an
} electron gun, contaminating it. TEM column is too tall to mount it } upside down, and fluorescence screen should be transparent. Horizontal } positioning will take much more space than vertical one, and again will
} introduce inconvenience in mounting SEM specimens and observation of } TEM screen. } } Physics allows any positioning of a column in space; ergonomics speaks } for traditional one, vertical with a gun on the top. } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } } -----Original Message----- } } From: liina170-at-hot.ee [mailto:liina170-at-hot.ee] } } Sent: Thursday, May 28, 2009 7:40 AM } } To: Dusevich, Vladimir } } Subject: [Microscopy] viaWWW: SEM and TEM Column Orientation } } } } } } } } } } -------------------------------------------------------------- } } -------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------- } } -------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://www.microscopy.com/MLFormMail.html } } -------------------------------------------------------------- } } ------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both liina170-at-hot.ee as well as the } } MIcroscopy Listserver } } -------------------------------------------------------------- } } ------------- } } } } Email: liina170-at-hot.ee } } Name: Liina } } } } Organization: University of Tartu } } } } Title-Subject: [Filtered] SEM and TEM } } } } Question: What would be the main advantages and disadvantages of a } } SEM that operates upside down i.e. has e-gun at the bottom pointing } } upwards. What about horizontal design? The same questions about TEM. } } } } } } Login Host: 213.184.38.91 } } -------------------------------------------------------------- } } ------------- } } } } ==============================Original } } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Thu May 28 07:38:53 2009 7, 11 } } -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id n4SCcqfd009244 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 28 May } } 2009 07:38:53 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240801c644363489d0-at-[206.69.208.22]} } } 7, 11 -- Date: Thu, 28 May 2009 07:38:52 -0500 7, 11 -- To: } } microscopy-at-microscopy.com 7, 11 -- From: liina170-at-hot.ee (by way of } } MicroscopyListserver) 7, 11 -- Subject: viaWWW: SEM and TEM Column } } Orientation 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; } } format="flowed" } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original } Headers============================== } 8, 25 -- From DusevichV-at-umkc.edu Thu May 28 07:58:56 2009 8, 25 -- } Received: from kc-msxproto1.kc.umkc.edu (smtp.exchange.umkc.edu } [134.193.143.167]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4SCwsIq007428 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 } 07:58:55 -0500 } 8, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by } kc-msxproto1.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 25 -- Thu, 28 May 2009 07:58:53 -0500 } 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 -- } Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain; } 8, 25 -- charset="us-ascii" } 8, 25 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column } Orientation 8, 25 -- Date: Thu, 28 May 2009 07:58:52 -0500 8, 25 -- } Message-ID: } {032EC4F75A527A4FA58C5B1B5DECFBB3062CB85F-at-KC-MSX1.kc.umkc.edu} } 8, 25 -- In-Reply-To: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column } Orientation 8, 25 -- Thread-Index: AcnfkV3lqn+L1Jn9TRuWenYm2Cn+tQAApCiA } 8, 25 -- References: {200905281239.n4SCda5s010569-at-ns.microscopy.com} } 8, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 25 -- To: } {liina170-at-hot.ee} , {Microscopy-at-microscopy.com} 8, 25 -- } X-OriginalArrivalTime: 28 May 2009 12:58:53.0022 (UTC) } FILETIME=[0D6DBFE0:01C9DF94] 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4SCwsIq007428 ==============================End } of - Headers==============================
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 10, 22 -- From colijn.1-at-osu.edu Thu May 28 09:18:15 2009 10, 22 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4SEIFud005161 10, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 May 2009 09:18:15 -0500 10, 22 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 22 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31556) 10, 22 -- id {01N9HDHXTPQO8XEUCW-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 10, 22 -- Thu, 28 May 2009 10:18:14 -0400 (EDT) 10, 22 -- Received: from HOC1.ecr6.ohio-state.edu 10, 22 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 10, 22 -- (PMDF V6.3-x18 #31556) with ESMTPA id {01N9HDHWLVVY8XKOCN-at-ecr6.ohio-state.edu} 10, 22 -- for Microscopy-at-microscopy.com; Thu, 28 May 2009 10:18:13 -0400 (EDT) 10, 22 -- Date: Thu, 28 May 2009 10:19:40 -0400 10, 22 -- X-from: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 22 -- Subject: Re: [Microscopy] RE: viaWWW: SEM and TEM Column Orientation 10, 22 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 22 -- To: Microscopy-at-microscopy.com 10, 22 -- Message-id: {01N9HDHWOAIO8XKOCN-at-ecr6.ohio-state.edu} 10, 22 -- MIME-version: 1.0 10, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 22 -- Content-type: text/plain; charset=us-ascii; format=flowed 10, 22 -- X-Env-From: auth/colijn.1-at-osu.edu ==============================End of - Headers==============================
==============================Original Headers============================== 24, 31 -- From Derek.Simpson-at-fei.com Fri May 29 07:49:05 2009 24, 31 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 24, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4TCn3L9005875 24, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 07:49:04 -0500 24, 31 -- X-WSS-ID: 0KKEOXQ-01-P2H-02 24, 31 -- X-M-MSG: 24, 31 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 24, 31 -- by smtp.feico.com (Tumbleweed MailGate 3.6.1) with ESMTP id 2CBEA82F7E7; 24, 31 -- Fri, 29 May 2009 05:49:02 -0700 (PDT) 24, 31 -- Received: from hlexc04.w2k.feico.com ([10.150.40.55]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 24, 31 -- Fri, 29 May 2009 05:49:00 -0700 24, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 24, 31 -- Content-class: urn:content-classes:message 24, 31 -- MIME-Version: 1.0 24, 31 -- Content-Type: text/plain; 24, 31 -- charset="us-ascii" 24, 31 -- Subject: RE: [Microscopy] viaWWW: SEM and TEM Column Orientation 24, 31 -- Date: Fri, 29 May 2009 05:47:58 -0700 24, 31 -- Message-ID: {A6E613CC1F9FD34B9A5EEE7DC4590AE302522DB0-at-hlexc04.w2k.feico.com} 24, 31 -- In-Reply-To: {200905281420.n4SEKo3Q011265-at-ns.microscopy.com} 24, 31 -- X-MS-Has-Attach: 24, 31 -- X-MS-TNEF-Correlator: 24, 31 -- Thread-Topic: [Microscopy] viaWWW: SEM and TEM Column Orientation 24, 31 -- thread-index: Acnfn4IHGwgMKj7FQGGCWpxtbb0YEgAAf6cA 24, 31 -- References: {200905281420.n4SEKo3Q011265-at-ns.microscopy.com} 24, 31 -- From: "Simpson, Derek" {Derek.Simpson-at-fei.com} 24, 31 -- To: {Microscopy-at-microscopy.com} 24, 31 -- Cc: {colijn.1-at-osu.edu} 24, 31 -- X-OriginalArrivalTime: 29 May 2009 12:49:00.0990 (UTC) FILETIME=[D6F695E0:01C9E05B] 24, 31 -- Content-Transfer-Encoding: 8bit 24, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TCn3L9005875 ==============================End of - Headers==============================
As Henk and others have alluded to there have been "inverted" and "tilted" columns for years. The highest resolution scanning tranmsision electron microsopes in the world (previously made by VG and now by NION) were all configured with the gun at the bottom. Although the new generation of aberation corrected are giving these now 20 year old instruments a run for their money in the area of image resolution.
My particuliar instrument the ANL AAEM/VG HB603Z which has its FEG at the bottom had been running since the early 90's. It is both a STEM and TEM and has a transmission screen at the top ~ 3 meters off the ground. Transmission screen is viewed using both TV and CCD cameras (no need to stand on your head).
Haven't yet found any conventional geometry (i.e. gun at the top) that can beat the microanalytical sensitivity of this design. But I will freely admit operating in standard TEM mode isn't as convenient as a machine primarily designed for that function. My 603Z was never intended to be a TEM, but rather an AEM.
The ANL 603Z is in the process of being decommissioned so for those of you that want to have a look at this geometry (while it is being slowly decommisssioned) here are 2 links.
http://tpm.amc.anl.gov Live streaming video of the AAEM Lab Select Macroscope & Video Source #1 Use Firefox or Safari WWW browsers
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Visiting Professor of Physics - Northern Illinois University
The past week has been one of the most enjoyable and informative periods I have ever seen on the server. In particular, note the discussions on inverted columns and N2. The threads have been informative, but at the same time there has been a bit of friendly humour - lighthearted banter.
I would like to thank the list, and in particular those who contributed to these two threads. It has been great.
paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009 7, 18 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFF96Y006040 7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:15:09 -0500 7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 7, 18 -- (authenticated bits=0) 7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n4TFF57l019281; 7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT) 7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca} 7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500 7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 7, 18 -- MIME-Version: 1.0 7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 7, 18 -- Subject: Inverted columns and N2 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 18 -- Content-Transfer-Encoding: 7bit 7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
Agreed, Paul. And as an aside, I would like to emphasize that ALL column orientations are welcome in our field. Electron microscopy has a tent big enough for all.
Cheers, Randy
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Friday, May 29, 2009 10:16 AM To: Tindall, Randy D.
Everyone
The past week has been one of the most enjoyable and informative periods
I have ever seen on the server. In particular, note the discussions on inverted columns and N2. The threads have been informative, but at the same time there has been a bit of friendly humour - lighthearted banter.
I would like to thank the list, and in particular those who contributed to these two threads. It has been great.
paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009 7, 18 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca [130.179.16.34]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFF96Y006040 7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:15:09 -0500 7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 7, 18 -- (authenticated bits=0) 7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id n4TFF57l019281; 7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT) 7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca} 7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500 7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 7, 18 -- MIME-Version: 1.0 7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 7, 18 -- Subject: Inverted columns and N2 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 18 -- Content-Transfer-Encoding: 7bit 7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 16, 30 -- From TindallR-at-missouri.edu Fri May 29 10:22:41 2009 16, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 16, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TFMeNK023300 16, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:22:41 -0500 16, 30 -- X-IronPort-Anti-Spam-Filtered: true 16, 30 -- X-IronPort-Anti-Spam-Result: ApoEADuaH0rRauUp/2dsb2JhbADDGwEJhk6IUQKCVYE1BQ 16, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 16, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 29 May 2009 10:22:40 -0500 16, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 30 -- Fri, 29 May 2009 10:22:39 -0500 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 30 -- Content-class: urn:content-classes:message 16, 30 -- MIME-Version: 1.0 16, 30 -- Content-Type: text/plain; 16, 30 -- charset="us-ascii" 16, 30 -- Subject: RE: [Microscopy] Inverted columns and N2 16, 30 -- Date: Fri, 29 May 2009 10:22:32 -0500 16, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD8134-at-UM-XMAIL08.um.umsystem.edu} 16, 30 -- In-Reply-To: {200905291516.n4TFGL0A008585-at-ns.microscopy.com} 16, 30 -- X-MS-Has-Attach: 16, 30 -- X-MS-TNEF-Correlator: 16, 30 -- Thread-Topic: [Microscopy] Inverted columns and N2 16, 30 -- Thread-Index: AcngcG7DYbYyR9o3QJCZQ5XTQYFPsAAAJcdA 16, 30 -- References: {200905291516.n4TFGL0A008585-at-ns.microscopy.com} 16, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 16, 30 -- To: {paul_hazelton-at-umanitoba.ca} 16, 30 -- Cc: {microscopy-at-microscopy.com} 16, 30 -- X-OriginalArrivalTime: 29 May 2009 15:22:39.0904 (UTC) FILETIME=[4DDD3A00:01C9E071] 16, 30 -- Content-Transfer-Encoding: 8bit 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TFMeNK023300 ==============================End of - Headers==============================
Microscopists, Maybe now is time to consider whether inverted columns increase or decrease the danger from nitrogen? (wink). Tobias
} } ---------------------------------------------------------------------------- } } Agreed, Paul. And as an aside, I would like to emphasize that ALL } column orientations are welcome in our field. Electron microscopy has a } tent big enough for all. } } Cheers, } Randy } } -----Original Message----- } X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] } Sent: Friday, May 29, 2009 10:16 AM } To: Tindall, Randy D. } Subject: [Microscopy] Inverted columns and N2 } }
} Q.html } ------------------------------------------------------------------------ } ---- } } Everyone } } The past week has been one of the most enjoyable and informative periods } } I have ever seen on the server. In particular, note the discussions on } inverted columns and N2. The threads have been informative, but at the } same time there has been a bit of friendly humour - lighthearted } banter. } } I would like to thank the list, and in particular those who contributed } to these two threads. It has been great. } } paul } } -- } Paul R. Hazelton, PhD } Viral Gastroenteritis Study Group } University of Manitoba } Department of Medical Microbiology } 511 Basic Medical Sciences Building } 745 William Avenue } Winnipeg, Manitoba, Canada, R3E 0J9 } e-mail: paul_hazelton-at-umanitoba.ca } paulhazelton-at-mts.net } Phone: 204-789-3313 (w); } 204-489-6924 (h) } Cell: 204-781-6982 } Fax: 204-789-3926 } } } } ==============================Original } Headers============================== } 7, 18 -- From paul_hazelton-at-umanitoba.ca Fri May 29 10:15:09 2009 } 7, 18 -- Received: from taygeta.cc.umanitoba.ca (taygeta.cc.umanitoba.ca } [130.179.16.34]) } 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4TFF96Y006040 } 7, 18 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 } 10:15:09 -0500 } 7, 18 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca } [140.193.25.69]) } 7, 18 -- (authenticated bits=0) } 7, 18 -- by taygeta.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id } n4TFF57l019281; } 7, 18 -- Fri, 29 May 2009 10:15:05 -0500 (CDT) } 7, 18 -- Message-ID: {4A1FFBF7.70704-at-umanitoba.ca} } 7, 18 -- Date: Fri, 29 May 2009 10:15:03 -0500 } 7, 18 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} } 7, 18 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) } 7, 18 -- MIME-Version: 1.0 } 7, 18 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } 7, 18 -- Subject: Inverted columns and N2 } 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 18 -- Content-Transfer-Encoding: 7bit } 7, 18 -- X-DCC-UofM-Metrics: taygeta; whitelist } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 16, 30 -- From TindallR-at-missouri.edu Fri May 29 10:22:41 2009 } 16, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu } (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) } 16, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id n4TFMeNK023300 } 16, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 } 10:22:41 -0500 } 16, 30 -- X-IronPort-Anti-Spam-Filtered: true } 16, 30 -- X-IronPort-Anti-Spam-Result: } ApoEADuaH0rRauUp/2dsb2JhbADDGwEJhk6IUQKCVYE1BQ } 16, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) } ([209.106.229.41]) } 16, 30 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 29 } May 2009 10:22:40 -0500 } 16, 30 -- Received: from UM-XMAIL08.um.umsystem.edu } ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 16, 30 -- Fri, 29 May 2009 10:22:39 -0500 } 16, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 16, 30 -- Content-class: urn:content-classes:message } 16, 30 -- MIME-Version: 1.0 } 16, 30 -- Content-Type: text/plain; } 16, 30 -- charset="us-ascii" } 16, 30 -- Subject: RE: [Microscopy] Inverted columns and N2 } 16, 30 -- Date: Fri, 29 May 2009 10:22:32 -0500 } 16, 30 -- Message-ID: } {91108EF9255B394CBF8B7E3789814A4103CD8134-at-UM-XMAIL08.um.umsystem.edu} } 16, 30 -- In-Reply-To: {200905291516.n4TFGL0A008585-at-ns.microscopy.com} } 16, 30 -- X-MS-Has-Attach: } 16, 30 -- X-MS-TNEF-Correlator: } 16, 30 -- Thread-Topic: [Microscopy] Inverted columns and N2 } 16, 30 -- Thread-Index: AcngcG7DYbYyR9o3QJCZQ5XTQYFPsAAAJcdA } 16, 30 -- References: {200905291516.n4TFGL0A008585-at-ns.microscopy.com} } 16, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 16, 30 -- To: {paul_hazelton-at-umanitoba.ca} } 16, 30 -- Cc: {microscopy-at-microscopy.com} } 16, 30 -- X-OriginalArrivalTime: 29 May 2009 15:22:39.0904 (UTC) } FILETIME=[4DDD3A00:01C9E071] } 16, 30 -- Content-Transfer-Encoding: 8bit } 16, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4TFMeNK023300 } ==============================End of - Headers==============================
I would like everyone to sign my petition that electron microscopy be designated as occurring in an upright column between one gun and one transmission screen. Any other orientation or configuration should have to be satisfied with civil union microscopy...(wink). (Sorry all, it's Friday, and I've had to cram 5 days of work into 4, so I'm getting a little silly)
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal.
-----Original Message-----
} Microscopists, } Maybe now is time to consider whether inverted columns } increase or decrease the danger from nitrogen? (wink). } Tobias } } } } } ---------------------------------------------------------------------- ---- } } } } Agreed, Paul. And as an aside, I would like to emphasize that ALL } } column orientations are welcome in our field. Electron microscopy has a } } tent big enough for all. } } } } Cheers, } } Randy
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==============================Original Headers============================== 9, 34 -- From Robert.Zonis-at-Sanford.com Fri May 29 10:54:13 2009 9, 34 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n4TFsC9O027153 9, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 29 May 2009 10:54:13 -0500 9, 34 -- X-VirusChecked: Checked 9, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 9, 34 -- X-Msg-Ref: server-13.tower-192.messagelabs.com!1243612445!38069439!11 9, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 9, 34 -- X-Originating-IP: [198.176.16.26] 9, 34 -- Received: (qmail 16150 invoked from network); 29 May 2009 15:54:12 -0000 9, 34 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) (198.176.16.26) 9, 34 -- by server-13.tower-192.messagelabs.com with SMTP; 29 May 2009 15:54:12 -0000 9, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 9, 34 -- Fri, 29 May 2009 10:53:51 -0500 9, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 34 -- Content-class: urn:content-classes:message 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; 9, 34 -- charset="us-ascii" 9, 34 -- Subject: RE: [Microscopy] RE: Inverted columns and N2 9, 34 -- Date: Fri, 29 May 2009 10:50:31 -0500 9, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A9402E72545-at-naoaksasebe02.nr.ad.newellco.com} 9, 34 -- In-Reply-To: {200905291534.n4TFYXSV014450-at-ns.microscopy.com} 9, 34 -- X-MS-Has-Attach: 9, 34 -- X-MS-TNEF-Correlator: 9, 34 -- Thread-Topic: [Microscopy] RE: Inverted columns and N2 9, 34 -- thread-index: AcngcvjDXwFgCgoQRPG31Bh2HV+6NwAANJ9g 9, 34 -- References: {200905291534.n4TFYXSV014450-at-ns.microscopy.com} 9, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 9, 34 -- To: {Microscopy-at-microscopy.com} 9, 34 -- Cc: {baskin-at-bio.umass.edu} 9, 34 -- X-OriginalArrivalTime: 29 May 2009 15:53:51.0642 (UTC) FILETIME=[A981C7A0:01C9E075] 9, 34 -- Content-Transfer-Encoding: 8bit 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TFsC9O027153 ==============================End of - Headers==============================
From bestsite103-at-hotmail.com Fri May 29 15:17:53 2009 Return-Path: {bestsite103-at-hotmail.com} Received: from google.com (59-104-156-240.adsl.dynamic.seed.net.tw [59.104.156.240]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TKHqAX007657 for {microscopylistserverarchive-at-microscopy.com} ; Fri, 29 May 2009 15:17:53 -0500 Received: from [119.71.63.188] (HELO google.com) by huge-ranked.org; Sat, 30 May 2009 04:17:44 +0800 Message-ID: {0000000473483A61293987394} Reply-To: Jerrard Joyce {monie.misty1185-at-gmail.com}
When I introduce our CM100 TEM to new users, I tell them it is configured like an inverted optical microscope. If that's the case, isn't the conventional TEM column really "inverted"???
All kidding aside, we've been calling it an "inverted column", which I still consider literally acceptable if the viewing screen or table is the baseline. Is it an actual industry standard for describing the more common TEM column arrangement "conventional" or "upright?" Is "inverted" also an acceptable industry-wide description? (It didn't seem to confuse anyone on this conversation.)
Regards, ~Gregg Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Friday, May 29, 2009 11:17 AM To: Sobocinski, Gregg
Colleagues;
As Henk and others have alluded to there have been "inverted" and "tilted" columns for years. The highest resolution scanning transmission electron microscopes in the world (previously made by VG and now by NION) were all configured with the gun at the bottom. Although the new generation of aberration corrected are giving these now 20 year old instruments a run for their money in the area of image resolution.
My particuliar instrument the ANL AAEM/VG HB603Z which has its FEG at the bottom had been running since the early 90's. It is both a STEM and TEM and has a transmission screen at the top ~ 3 meters off the ground. Transmission screen is viewed using both TV and CCD cameras (no need to stand on your head).
Haven't yet found any conventional geometry (i.e. gun at the top) that can beat the microanalytical sensitivity of this design. But I will freely admit operating in standard TEM mode isn't as convenient as a machine primarily designed for that function. My 603Z was never intended to be a TEM, but rather an AEM.
The ANL 603Z is in the process of being decommissioned so for those of you that want to have a look at this geometry (while it is being slowly decommisssioned) here are 2 links.
http://tpm.amc.anl.gov Live streaming video of the AAEM Lab Select Macroscope & Video Source #1 Use Firefox or Safari WWW browsers
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Visiting Professor of Physics - Northern Illinois University
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Title-Subject: [Filtered] TEM Sample Preparation Charges
Question: Dear All, By the way, as introduction I should say that I'm in charge of the EM part of the Sussex Centre for Advanced Microscopy here in Life Sciences at the University of Sussex in Brighton UK. Anyway, I've recently had the supervisor of a postgrad student I've been helping (to prepare leaf tissues treated in various ways to examine effects upon chloroplast morphology) query my charging and suggesting it was overpriced. Here, we charge £10 per sample (if processed by standard double-fixation approaches, dehydration and embedding in TAAB low viscosity resin). And by 'per sample', I mean as many pieces of tissue (e.g. replicates of a given treatment) that can be processed through in one glass vial. I thought this was entirely reasonable. Can you folks out there give me some examples of your pricing for similar preparations, if you're happy disclosing that of course! Otherwise, just some general feedback would be useful. Thanks in advance for your help, Jules
Dr. Julian R. Thorpe Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
I'm not in biology or medicine but, I still think that you should charge more than what you are currently charging. Because, you are using your expertise. If the other party thinks your pricing is high then he/she should obtain the expertise and prepare his samples by himself.
Sorry, I jumped in even though it is not my field of expertise. Because, it touched a nerve. I come across researchers who thinks that TEM work is not a scientific contribution even when used in a research paper.
At the moment, I'm doing TEM investigation for all the researchers. We do not have enough trained people and we do not have the system in place where I would give basic TEM training to a new user and leave him on his own. Hopefully, we will have this system later on.
Recently, I had an inquiry that one researcher wants me to find out size distribution of metal nano-particles which are 0.4 - 1.0 nm in size based on XRD study. It sounds a lot hard to find them in the first place. So, I told him that I can undertake this study only if I will be included in the paper as co-author. He did not agree and said that "You will give me two images. What is the scientific contribution here?"
Regards, Ayten.
-- =========================== Ayten Celik-Aktas, PhD Ankara University Electron Microscopy Laboratory Ankara, Turkey ===========================
On Sat, May 30, 2009 at 1:27 AM, {j.r.thorpe-at-sussex.ac.uk} wrote: } } } Email: j.r.thorpe-at-sussex.ac.uk } Name: Julian Thorpe } } Organization: University of Sussex } } Title-Subject: [Filtered] TEM Sample Preparation Charges } } Question: Dear All, } By the way, as introduction I should say that I'm } in charge of the EM part of the Sussex Centre for } Advanced Microscopy here in Life Sciences at the } University of Sussex in Brighton UK. } Anyway, I've recently had the supervisor of a } postgrad student I've been helping (to prepare } leaf tissues treated in various ways to examine } effects upon chloroplast morphology) query my } charging and suggesting it was overpriced. } Here, we charge £10 per sample (if processed by } standard double-fixation approaches, dehydration } and embedding in TAAB low viscosity resin). And } by 'per sample', I mean as many pieces of tissue } (e.g. replicates of a given treatment) that can } be processed through in one glass vial. } I thought this was entirely reasonable. } Can you folks out there give me some examples of } your pricing for similar preparations, if you're } happy disclosing that of course! Otherwise, just } some general feedback would be useful. } Thanks in advance for your help, } Jules } } Dr. Julian R. Thorpe } Electron Microscope Division, } The Sussex Centre for Advanced Microscopy, } John Maynard-Smith Building, } School of Life Sciences, } University of Sussex, } Falmer, } Brighton BN1 9QG } Tel.: ext +44 (0)1273 877585 }    int 7585 } } URLs: } (home) } http://www.sussex.ac.uk/biology/profile2686.html } (lab) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm } (research) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm } }  Login Host: 139.184.30.134 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 9, 13 -- From zaluzec-at-microscopy.com Fri May 29 17:21:52 2009 } 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 13 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TMLpoX032550 } 9, 13 --     for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 17:21:52 -0500 } 9, 13 -- Mime-Version: 1.0 } 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]} } 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500 } 9, 13 -- To: microscopy-at-microscopy.com } 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver) } 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges } 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 9, 13 -- Content-Transfer-Encoding: 8bit } 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TMLpoX032550 } ==============================End of - Headers==============================
==============================Original Headers============================== 16, 36 -- From celikaktas-at-gmail.com Sat May 30 07:55:53 2009 16, 36 -- Received: from mail-fx0-f212.google.com (mail-fx0-f212.google.com [209.85.220.212]) 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4UCtqxm026348 16, 36 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2009 07:55:53 -0500 16, 36 -- Received: by fxm8 with SMTP id 8so7876581fxm.18 16, 36 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2009 05:55:52 -0700 (PDT) 16, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 36 -- d=gmail.com; s=gamma; 16, 36 -- h=domainkey-signature:mime-version:received:in-reply-to:references 16, 36 -- :date:message-id:subject:from:to:cc:content-type 16, 36 -- :content-transfer-encoding; 16, 36 -- bh=c+7cZgjMLGWY2e4EBUnaCifFlQ/h5JNNbTr314T+gyU=; 16, 36 -- b=lyWnxbyvNc5XclMvVmbs3gZbpJ2dFZbp039knhWliNdaeGXBykWNiVAKOEnP4j7qYv 16, 36 -- tPZ/L2immyG65ujQHnBurhE/ou2woRNGCey0K0ZtIPaHAzJIbC//tMx1hstw53z5Xxey 16, 36 -- T3mH+wrWPEtOwByznd61HhQgg9M/g010wIJtU= 16, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 16, 36 -- d=gmail.com; s=gamma; 16, 36 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 16, 36 -- :cc:content-type:content-transfer-encoding; 16, 36 -- b=q4xT1TLGUijMOHUHftLA6Mk4Xg1228xBun0obP6dFPJtePMlGl093Z+Afm3XpMACDD 16, 36 -- Jpr373wfE8nodF6snhBwhv4wtved3cT7GMsAD2fp45JcaZmZI5QLjIDdB/rQ8Nd5io6d 16, 36 -- ZqWXu2FSKPQMX+O9CEhlX05PVgrpezSFx9B00= 16, 36 -- MIME-Version: 1.0 16, 36 -- Received: by 10.103.223.2 with SMTP id a2mr2249133mur.4.1243688152197; Sat, 30 16, 36 -- May 2009 05:55:52 -0700 (PDT) 16, 36 -- In-Reply-To: {200905292227.n4TMRolY010992-at-ns.microscopy.com} 16, 36 -- References: {200905292227.n4TMRolY010992-at-ns.microscopy.com} 16, 36 -- Date: Sat, 30 May 2009 15:55:52 +0300 16, 36 -- Message-ID: {1075c5c10905300555m3efb167ek7c0d77482e9ba55e-at-mail.gmail.com} 16, 36 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges 16, 36 -- From: Ayten Celik-Aktas {celikaktas-at-gmail.com} 16, 36 -- To: j.r.thorpe-at-sussex.ac.uk 16, 36 -- Cc: microscopy {Microscopy-at-microscopy.com} 16, 36 -- Content-Type: text/plain; charset=UTF-8 16, 36 -- Content-Transfer-Encoding: 8bit 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4UCtqxm026348 ==============================End of - Headers==============================
Due to the extremely wide variety of samples we are asked to process, we do not have a flat rate, but charge for chemicals and for technician time by the hour. We made up an informative sort of spreadsheet that helps prospective clients see how long it will take (sometimes the most important part when they want TEM!) and where the charges are, so that they can decide if they want to do any, part, or all of it themselves.
Here's the bottom line for fixation to a few images on the TEM: $411 to $645 (USD) for the first sample, plus another couple hundred dollars EACH for more samples done at the same time! About $250 - $450 just to get them into blocks, primarily because we charge $43/hour for tech time. It is USUALLY much cheaper than this because they do not have to pay for me to stand at the fume hood doing nothing while waiting to change to the next alcohol, BUT they need to be prepared for this, if necessary.
So show them this and tell them they are getting a real bargain.
Aloha, Tina
} Question: Dear All, } By the way, as introduction I should say that I'm } in charge of the EM part of the Sussex Centre for } Advanced Microscopy here in Life Sciences at the } University of Sussex in Brighton UK. } Anyway, I've recently had the supervisor of a } postgrad student I've been helping (to prepare } leaf tissues treated in various ways to examine } effects upon chloroplast morphology) query my } charging and suggesting it was overpriced. } Here, we charge £10 per sample (if processed by } standard double-fixation approaches, dehydration } and embedding in TAAB low viscosity resin). And } by 'per sample', I mean as many pieces of tissue } (e.g. replicates of a given treatment) that can } be processed through in one glass vial. } I thought this was entirely reasonable. } Can you folks out there give me some examples of } your pricing for similar preparations, if you're } happy disclosing that of course! Otherwise, just } some general feedback would be useful. } Thanks in advance for your help, } Jules } } Dr. Julian R. Thorpe } Electron Microscope Division, } The Sussex Centre for Advanced Microscopy, } John Maynard-Smith Building, } School of Life Sciences, } University of Sussex, } Falmer, } Brighton BN1 9QG } Tel.: ext +44 (0)1273 877585 } int 7585 } } URLs: } (home) } http://www.sussex.ac.uk/biology/profile2686.html } (lab) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm } (research) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm } } Login Host: 139.184.30.134 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 9, 13 -- From zaluzec-at-microscopy.com Fri May 29 17:21:52 2009 } 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4TMLpoX032550 } 9, 13 -- for {microscopy-at-microscopy.com} ; Fri, 29 May 2009 17:21:52 -0500 } 9, 13 -- Mime-Version: 1.0 } 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]} } 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500 } 9, 13 -- To: microscopy-at-microscopy.com } 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver) } 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges } 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 9, 13 -- Content-Transfer-Encoding: 8bit } 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n4TMLpoX032550 } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 9, 23 -- From tina-at-pbrc.hawaii.edu Sat May 30 17:34:49 2009 9, 23 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4UMYmuD010985 9, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 30 May 2009 17:34:48 -0500 9, 23 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 9, 23 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id n4UMYh6Y007963 9, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 9, 23 -- Sat, 30 May 2009 12:34:44 -1000 (HST) 9, 23 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id n4UMYfCo007960; 9, 23 -- Sat, 30 May 2009 12:34:42 -1000 (HST) 9, 23 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 9, 23 -- Date: Sat, 30 May 2009 12:34:40 -1000 (HST) 9, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 9, 23 -- X-Sender: tina-at-halia 9, 23 -- To: j.r.thorpe-at-sussex.ac.uk 9, 23 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 23 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges 9, 23 -- In-Reply-To: {200905292222.n4TMMux0001634-at-ns.microscopy.com} 9, 23 -- Message-ID: {Pine.GSO.4.21.0905301221460.7927-100000-at-halia} 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: TEXT/PLAIN; charset=X-UNKNOWN 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id n4UMYmuD010985 ==============================End of - Headers==============================
Dear Bradford, Derrick, Paula, Teresa, Dale, Shashi, Ayten, Jim, Tina, John, Rosemary and anyone else I may have left out!
Many thanks for all your feedback on my post. Very useful, thank you, and I feel more vindicated now that I am certainly not overcharging for my TEM preparation!
Cheers, Jules
On 29-May-09, at 3:25 PM, j.r.thorpe-at-sussex.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both j.r.thorpe-at-sussex.ac.uk as } well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: j.r.thorpe-at-sussex.ac.uk } Name: Julian Thorpe } } Organization: University of Sussex } } Title-Subject: [Filtered] TEM Sample Preparation Charges } } Question: Dear All, } By the way, as introduction I should say that I'm } in charge of the EM part of the Sussex Centre for } Advanced Microscopy here in Life Sciences at the } University of Sussex in Brighton UK. } Anyway, I've recently had the supervisor of a } postgrad student I've been helping (to prepare } leaf tissues treated in various ways to examine } effects upon chloroplast morphology) query my } charging and suggesting it was overpriced. } Here, we charge £10 per sample (if processed by } standard double-fixation approaches, dehydration } and embedding in TAAB low viscosity resin). And } by 'per sample', I mean as many pieces of tissue } (e.g. replicates of a given treatment) that can } be processed through in one glass vial. } I thought this was entirely reasonable. } Can you folks out there give me some examples of } your pricing for similar preparations, if you're } happy disclosing that of course! Otherwise, just } some general feedback would be useful. } Thanks in advance for your help, } Jules } } Dr. Julian R. Thorpe } Electron Microscope Division, } The Sussex Centre for Advanced Microscopy, } John Maynard-Smith Building, } School of Life Sciences, } University of Sussex, } Falmer, } Brighton BN1 9QG } Tel.: ext +44 (0)1273 877585 } int 7585 } } URLs: } (home) } http://www.sussex.ac.uk/biology/profile2686.html } (lab) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm } (research) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm
==============================Original Headers============================== 7, 24 -- From bafg3-at-sussex.ac.uk Mon Jun 1 04:24:59 2009 7, 24 -- Received: from chip.uscs.susx.ac.uk (chip.uscs.susx.ac.uk [139.184.14.86]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n519OuY1002821 7, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2009 04:24:58 -0500 7, 24 -- Received: from ls0130.lifesci.susx.ac.uk ([139.184.162.69]:2040) 7, 24 -- by chip.uscs.susx.ac.uk with esmtp (Exim 4.64) 7, 24 -- (envelope-from {bafg3-at-sussex.ac.uk} ) 7, 24 -- id KKJZI9-00044S-HL 7, 24 -- for Microscopy-at-microscopy.com; Mon, 01 Jun 2009 10:25:21 +0100 7, 24 -- Date: Mon, 01 Jun 2009 10:21:34 +0100 7, 24 -- To: Microscopy-at-microscopy.com 7, 24 -- Subject: Re: [Microscopy] viaWWW: TEM Sample Preparation Charges 7, 24 -- Message-ID: {2606146295.1243851694-at-ls0130.lifesci.susx.ac.uk} 7, 24 -- Originator-Info: login-token=Mulberry:01TqCG1rAB7D2C4OYwtwR8E/tJwgXx/a/7fBXx; 7, 24 -- token_authority=postmaster-at-central.susx.ac.uk 7, 24 -- X-Mailer: Mulberry/2.0.8 (Win32) 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; charset=iso-8859-1; format=flowed 7, 24 -- Content-Disposition: inline 7, 24 -- X-Sussex: true 7, 24 -- X-Sussex-transport: remote_smtp_rew 7, 24 -- From: Julian Thorpe {j.r.thorpe-at-sussex.ac.uk} 7, 24 -- Content-Transfer-Encoding: 8bit 7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n519OuY1002821 ==============================End of - Headers==============================
We have received some post-mortem FROZEN human brain pieces which we would like to aldehyde fix and embed in sucrose for Tokuyasu cryosectioning. Could anyone recommend the best fixative and fixation procedure?
Would it work to just transfer the frozen tissue samples (0.5cm wide) to melt in a buffered formaldehyde solution, possibly in the presence of sucrose or another cryoprotectant? Would it be best to have the fixative at 4oC for slow melting or at room temperature?
Thanks in advance, Ana and Sheila
==============================Original Headers============================== 5, 19 -- From marjolein.snippe-at-csc.mrc.ac.uk Mon Jun 1 10:26:43 2009 5, 19 -- Received: from smtp1.cc.ic.ac.uk (smtp1.cc.ic.ac.uk [155.198.5.155]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n51FQhil031720 5, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Jun 2009 10:26:43 -0500 5, 19 -- Received: from default.csc.mrc.ac.uk ([193.60.222.2] helo=[10.23.5.51]) 5, 19 -- by smtp1.cc.ic.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 5, 19 -- (Exim 4.69) 5, 19 -- (envelope-from {marjolein.snippe-at-csc.mrc.ac.uk} ) 5, 19 -- id 1MB9PO-0005uQ-LR 5, 19 -- for Microscopy-at-microscopy.com; Mon, 01 Jun 2009 16:26:42 +0100 5, 19 -- Message-ID: {4A23F332.7020908-at-imperial.ac.uk} 5, 19 -- Date: Mon, 01 Jun 2009 16:26:42 +0100 5, 19 -- From: Marjolein Snippe {marjolein.snippe-at-csc.mrc.ac.uk} 5, 19 -- User-Agent: Thunderbird 2.0.0.21 (Windows/20090302) 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: EM Tokuyasu preparation 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Thanks Randy. The truth is that these charges will definitely go up once people with research funds (hopefully!) successfully acquired for projects that have been 'fully economically costed', FEC as we say here, come through the system. At present I am just about able to cover my service contracts on my TEM and the associated camera with the income generated in my lab. Jules
--On 01 June 2009 08:33 -0500 "Tindall, Randy D." {TindallR-at-missouri.edu} wrote:
} I agree with the others. Your service is extremely cheap. We couldn't } begin to cover the costs of our service with fees like you charge. } } We occasionally get complaints about price (normally from researchers } from other countries for some reason), but I just calmly explain the } steps we go through and show them some corporate rates for EM and they } usually quiet down. } } Good luck. } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=W } eek&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com } } } } -----Original Message----- } From: j.r.thorpe-at-sussex.ac.uk [mailto:j.r.thorpe-at-sussex.ac.uk] } Sent: Friday, May 29, 2009 5:23 PM } To: Tindall, Randy D. } Subject: [Microscopy] viaWWW: TEM Sample Preparation Charges } } } } } ------------------------------------------------------------------------- } --- The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------- } --- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ------------------------------------------------------------------------- } -- Remember this posting is most likely not from a Subscriber, so when } replying please copy both j.r.thorpe-at-sussex.ac.uk as } well as the MIcroscopy Listserver } ------------------------------------------------------------------------- } -- } } Email: j.r.thorpe-at-sussex.ac.uk } Name: Julian Thorpe } } Organization: University of Sussex } } Title-Subject: [Filtered] TEM Sample Preparation Charges } } Question: Dear All, } By the way, as introduction I should say that I'm } in charge of the EM part of the Sussex Centre for } Advanced Microscopy here in Life Sciences at the } University of Sussex in Brighton UK. } Anyway, I've recently had the supervisor of a } postgrad student I've been helping (to prepare } leaf tissues treated in various ways to examine } effects upon chloroplast morphology) query my } charging and suggesting it was overpriced. } Here, we charge £10 per sample (if processed by } standard double-fixation approaches, dehydration } and embedding in TAAB low viscosity resin). And } by 'per sample', I mean as many pieces of tissue } (e.g. replicates of a given treatment) that can } be processed through in one glass vial. } I thought this was entirely reasonable. } Can you folks out there give me some examples of } your pricing for similar preparations, if you're } happy disclosing that of course! Otherwise, just } some general feedback would be useful. } Thanks in advance for your help, } Jules } } Dr. Julian R. Thorpe } Electron Microscope Division, } The Sussex Centre for Advanced Microscopy, } John Maynard-Smith Building, } School of Life Sciences, } University of Sussex, } Falmer, } Brighton BN1 9QG } Tel.: ext +44 (0)1273 877585 } int 7585 } } URLs: } (home) } http://www.sussex.ac.uk/biology/profile2686.html } (lab) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm } (research) } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm } } Login Host: 139.184.30.134 } ------------------------------------------------------------------------- } -- } } } ==============================Original } Headers============================== 9, 13 -- From } zaluzec-at-microscopy.com Fri May 29 17:21:52 2009 } 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n4TMLpoX032550 9, 13 -- for {microscopy-at-microscopy.com} ; } Fri, 29 May 2009 17:21:52 -0500 9, 13 -- Mime-Version: 1.0 } 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]} } 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500 } 9, 13 -- To: microscopy-at-microscopy.com } 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver) } 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges } 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 9, 13 -- Content-Transfer-Encoding: 8bit } 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n4TMLpoX032550 ==============================End of } - Headers==============================
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
Hi Lee, thanks for that and please see my reply to Randy...... Jules
--On 01 June 2009 09:45 -0400 Leona Cohen-Gould {lcgould-at-med.cornell.edu} wrote:
} Dear Jules- } Even with the exchange rate what it is, I can tell you that he's getting } a bargain. My standard fee for processing, embedding, sectioning and } contrasting the grids (in LX112, EMbed 812 or Spurr's) is $125/sample, } with my definition of "sample" matching yours. Preps for immuno are } billed at $200/sample, plus labelling fees. You will always encounter } people who squawk at your pricing...I have people here who think that } because we are a College sponsored Core Facility they should not have to } pay anything at all! I don't know where they think the funds for things } like salaries, materials, chemicals, equipment, equipment maintenance, } etc come from! Good luck, } Lee } } } ------------------------------------------------------------------------ } } ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ } } ---- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } ------------------------------------------------------------------------ } } --- Remember this posting is most likely not from a Subscriber, so when } } replying please copy both j.r.thorpe-at-sussex.ac.uk as } } well as the MIcroscopy Listserver } } ------------------------------------------------------------------------ } } --- } } } } Email: j.r.thorpe-at-sussex.ac.uk } } Name: Julian Thorpe } } } } Organization: University of Sussex } } } } Title-Subject: [Filtered] TEM Sample Preparation Charges } } } } Question: Dear All, } } By the way, as introduction I should say that I'm } } in charge of the EM part of the Sussex Centre for } } Advanced Microscopy here in Life Sciences at the } } University of Sussex in Brighton UK. } } Anyway, I've recently had the supervisor of a } } postgrad student I've been helping (to prepare } } leaf tissues treated in various ways to examine } } effects upon chloroplast morphology) query my } } charging and suggesting it was overpriced. } } Here, we charge £10 per sample (if processed by } } standard double-fixation approaches, dehydration } } and embedding in TAAB low viscosity resin). And } } by 'per sample', I mean as many pieces of tissue } } (e.g. replicates of a given treatment) that can } } be processed through in one glass vial. } } I thought this was entirely reasonable. } } Can you folks out there give me some examples of } } your pricing for similar preparations, if you're } } happy disclosing that of course! Otherwise, just } } some general feedback would be useful. } } Thanks in advance for your help, } } Jules } } } } Dr. Julian R. Thorpe } } Electron Microscope Division, } } The Sussex Centre for Advanced Microscopy, } } John Maynard-Smith Building, } } School of Life Sciences, } } University of Sussex, } } Falmer, } } Brighton BN1 9QG } } Tel.: ext +44 (0)1273 877585 } } int 7585 } } } } URLs: } } (home) } } http://www.sussex.ac.uk/biology/profile2686.html } } (lab) } } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/cover.htm } } (research) } } http://www.lifesci.susx.ac.uk/Home/Julian_Thorpe/ad_cover.htm } } } } Login Host: 139.184.30.134 } } ------------------------------------------------------------------------ } } --- } } } } } } ==============================Original } } Headers============================== 9, 13 -- From } } zaluzec-at-microscopy.com Fri May 29 17:21:52 2009 } } 9, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) 9, 13 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } n4TMLpoX032550 } } 9, 13 -- for {microscopy-at-microscopy.com} ; } } Fri, 29 May 2009 17:21:52 -0500 } } 9, 13 -- Mime-Version: 1.0 } } 9, 13 -- Message-Id: {p06240801c6461069b079-at-[206.69.208.22]} } } 9, 13 -- Date: Fri, 29 May 2009 17:21:50 -0500 } } 9, 13 -- To: microscopy-at-microscopy.com } } 9, 13 -- From: j.r.thorpe-at-sussex.ac.uk (by way of MicroscopyListserver) } } 9, 13 -- Subject: viaWWW: TEM Sample Preparation Charges } } 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } } 9, 13 -- Content-Transfer-Encoding: 8bit } } 9, 13 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com id } } n4TMLpoX032550 } } ==============================End of - } } Headers============================== } } } -- } Lee Cohen-Gould, M.S. } Sr. Staff Associate in Biochemistry and } Cell & Developmental Biology } Director, Electron Microscopy & Histology } and Optical Microscopy Core Facilities } Weill Cornell Medical College } } voice (212)746-6146 } fax (212)746-8175 } http://www.med.cornell.edu/research/rea_sup/ } http://www.cornellcelldevbiology.org } http://www.cornellbiochem.org
Dr. Julian R. Thorpe (Office 2C9/Lab 2C11-13) Electron Microscope Division, The Sussex Centre for Advanced Microscopy, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG Tel.: ext +44 (0)1273 877585 int 7585
Late replying to this thread (it was half term here last week so I was at home) but it bought back happy memories of my first experience of using a TEM. More years ago than I care to remember I started as a trainee technician in the EM lab of the school of biological science at Leicester University and first learnt to use an AEI Corinth. The only problem I had initially was banging my knees against the objective aperture control! Happy days.
Nikki Weston
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==============================Original Headers============================== 5, 35 -- From Nicola.Weston-at-nottingham.ac.uk Tue Jun 2 04:08:42 2009 5, 35 -- Received: from ixe-mta-23.emailfiltering.com (ixe-mta-23-tx.emailfiltering.com [194.116.199.156]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n5298fei018409 5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jun 2009 04:08:41 -0500 5, 35 -- Received: from smtp2.nottingham.ac.uk ([128.243.44.5]) 5, 35 -- by ixe-mta-23.emailfiltering.com with emfmta (version 3.7.1.44.1.r-3.2.3-libc2.3.2) vanilla id 8320690 5, 35 -- for microscopy-at-microscopy.com; Tue, 02 Jun 2009 10:08:39 +0100 5, 35 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 5, 35 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 5, 35 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 5, 35 -- id 1MBPyX-00042X-PP 5, 35 -- for microscopy-at-microscopy.com; Tue, 02 Jun 2009 10:08:05 +0100 5, 35 -- Received: from VUIEXCHA.ad.nottingham.ac.uk ([128.243.44.231]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 5, 35 -- Tue, 2 Jun 2009 10:07:07 +0100 5, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 35 -- Content-class: urn:content-classes:message 5, 35 -- MIME-Version: 1.0 5, 35 -- Content-Type: text/plain; 5, 35 -- charset="us-ascii" 5, 35 -- Subject: SEM and TEM Column Orientation 5, 35 -- Date: Tue, 2 Jun 2009 10:07:07 +0100 5, 35 -- Message-ID: {1E0CCC81FDE82D4C8DDE1A8F44080D94018C4B93-at-VUIEXCHA.ad.nottingham.ac.uk} 5, 35 -- X-MS-Has-Attach: 5, 35 -- X-MS-TNEF-Correlator: 5, 35 -- Thread-Topic: SEM and TEM Column Orientation 5, 35 -- Thread-Index: AcnjYYEyQQfWtYTkSfeDKysO3s7Npw== 5, 35 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 5, 35 -- To: {microscopy-at-microscopy.com} 5, 35 -- X-OriginalArrivalTime: 02 Jun 2009 09:07:08.0075 (UTC) FILETIME=[817FF7B0:01C9E361] 5, 35 -- X-UoN-MailScanner-Information: Please contact staff-it-helpline-at-nottingham.ac.uk for more information 5, 35 -- X-UoN-MailScanner: Found to be clean 5, 35 -- X-UoN-MailScanner-From: nicola.weston-at-nottingham.ac.uk 5, 35 -- X-Spam-Status: No 5, 35 -- Content-Transfer-Encoding: 8bit 5, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n5298fei018409 ==============================End of - Headers==============================
I meant to chat to Natalie yesterday, as she normally does the inductions [safety talk/registration]. She's quite busy at the moment, so I can do the induction if she's unavailable. How about tomorrow [Wednesday] morning/afternoon? Say 10.00 or 2.00.
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: Michael Mccarthy [mailto:michael.mccarthy-at-balliol.ox.ac.uk] Sent: 01 June 2009 15:20 To: Keith Morris
Hi all,
Sorry for the last email. I meant to send it to our Core staff via microscopy-at-well.ox.ac.uk. Been carefully avoiding this for two years, but finally it happened [twice now, once to the confocal list-server].
Following on from this thread, if you want to delete an email address etc.. from an auto-complete tab do the following:
Delete a name from the AutoComplete list: Select the unwanted name using the UP ARROW or DOWN ARROW. Press DELETE.
Turn off AutoComplete name suggesting completely in Outlook: On the Tools menu, click Options. On the Preferences tab, click E-mail Options, and then click Advanced E-mail Options. Clear the Suggest names while completing To, Cc, and Bcc fields check box.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both schaffne-at-patho.unibe.ch as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: schaffne-at-patho.unibe.ch Name: Thomas Schaffner
Organization: Dept. of Pathology University of Bern Switzerland
Title-Subject: [Filtered] reembedment of thick epon sections
Question: Hallo Se Pyo
we have used a diamond pen to mark/scratch the interesting area on the bottom of the slide carrying the thick section. Then we removed the coverslip with xylene and carried the thick section through the terminal solvents and embedding mixtures of our preferred resin to finally cover the area of interest with a BEEM or other capsule turned upside down (you have to cut the lid off). The scratch on the bottom of the slide serves to center the capsule since the dye might have largely come off the section. You then cure the new block on the slide. To remove the block from the glass after curing place the slide on dry ice for a minute then immerse it in luke warm water for a minute. Repeat this cold/warm sequence patiently until the block falls off. Greetings from Switzerland
Thomas Schaffner Professor of Pathology University of Bern
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Email: rojasvidovic-at-unina.it Name: Juan Carlos Rojas
Organization: University of Glasgow
Title-Subject: [Filtered] freeze cutting
Question: Dear all,
Perhaps you can help me (or direct to an appropriate source) with this query ...
I'm interested in conduct Environmenatl Scanning Electron Microscopy (ESEM) analysis to study microstructures of fine grained soils.
Specimen preparation for ESEM analysis includes the creation of a flat and smooth observation surface. In order to minimise microstructural changes I will use the freeze cut method.
In the freeze cut method, a soil specimen is cut in a circular plate shape with a diameter of 18 mm and a heigth of 3 mm. The specimen is dipped into liquid nitrogen. The specimen is then cut into two parts with a cooled sharp knife to generate, as mentioned above, a smooth flat surface.
My question is: What kind of knife should I use?. Searching in internet I found some high quality single-edge razor blades but were not designed to cut ice.
Any clarification (or direction towards appropriate sources) will be very helpful. Thanks for your time.
Dear all (with apologies for duplicate posting to those of you who belong to both the confocal and microscopy listservs) We're about to start a series of correlative experiments relating confocal fluorescence to info from LM sections. We're using AlexaFluor488 and AlexaFluor568, and using wholemounts of small (2mm by 200µm) worms. The current plan is to photoconvert the dye using DAB, dehydrate, and embed in either paraffin or "Epon"/Araldite for sectioning. I've found plenty of protocols for DAB photoconversion, and we're starting those experiments this week. Does anyone know of a protocol for the photoconversion of another readily-oxidized dye to give a different color (other than the brown of DAB), so we can see both dyes at once in our sections? TIA, Julian
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
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Email: RonMervis-at-neurostructural.org Name: Ron Mervis
Organization: Neurostructural Research Labs
Title-Subject: [Filtered] double images in microscope
Question: good morning... i have a new student to whom i am teaching microscopy...we have Zeiss brightfield research microscopes... the problem i seem to have with him (and which i haven't had with others) is that he consistently sees double images which overlap and which he can't seem to get to converge... the oculars are adjustable and he has moved them around to try to remedy the problem -- but it hasn't helped...he occasionally wears glasses (he's a bit farsighted)...but otherwise his vision is perfectly normal...
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Email: rana.kasim-at-ist.utl.pt Name: Rana Kasim
Organization: Ph.D student Instituto Superior TÈcnicoMaterials Engineering Department-university of lisbon
Title-Subject: [Filtered] Monte carlo simulation with EDS analysis
Question: Hi All, Could any person help me to how i can get the Monte carlo simulation with Energy Dispersive Spectroscopy(EDS)and the software that can calculate the light volume when transmitt inside the tissue and its pentration depth.
Does this probleme occur on equal which microscope, or always, independently of the microsocpe ?
In case it's microscope independent, maybe it's an accomodation diffucly. Tell him he must try to look /"trought"/ the microscope, and not /"in"/ the microscope. The eyes must be relaxed, as if he would be looking at infinity, and not trying to accomodate at short distance. Some people have difficulties to do this. He can try, siting at the microscope, to look first out trough the window on something far away, an then to go to the oculars. Of coarse the interpupillar distance must be right. But for that, he can ask to an optician to measure it, and set this measured value on the microscope head, as a starting point.
In case it is only on one specific microscope, it could be that the binocular head has a collimation error, that he cannot compensate. Other users won't have the difficulty as they are familiar with the microscope, or are more trained to compensate. If it is the case, you would have interesst to correct it. A collimation error can be a big source of weariness, eyestrain and headeach. But such errors aren't frequent on OM, more on consumer type binoculars.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
RonMervis-at-neurostructural.org a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both RonMervis-at-neurostructural.org as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: RonMervis-at-neurostructural.org } Name: Ron Mervis } } Organization: Neurostructural Research Labs } } Title-Subject: [Filtered] double images in microscope } } Question: good morning... } i have a new student to whom i am teaching microscopy...we have Zeiss } brightfield research microscopes... } the problem i seem to have with him (and which i haven't had with } others) is that he consistently sees double images which overlap and } which he can't seem to get to converge... } the oculars are adjustable and he has moved them around to try to } remedy the problem -- but it hasn't helped...he occasionally wears } glasses (he's a bit farsighted)...but otherwise his vision is } perfectly normal... } } any suggestions??? } } thanks... } ron mervis } } Login Host: 64.12.117.15 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Wed Jun 3 20:28:46 2009 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n541Sjuw031726 } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun 2009 20:28:45 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c64cd3b17d4d-at-[206.69.208.22]} } 8, 11 -- Date: Wed, 3 Jun 2009 20:28:44 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: RonMervis-at-neurostructural.org (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: double images in microscope } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 32 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Jun 4 02:54:43 2009 12, 32 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n547sge4015897 12, 32 -- for {microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 02:54:42 -0500 12, 32 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 32 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id n547seKE016886 12, 32 -- ; Thu, 4 Jun 2009 09:54:40 +0200 (CEST) 12, 32 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 12, 32 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 12, 32 -- (No client certificate requested) 12, 32 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 967CF100021F; 12, 32 -- Thu, 4 Jun 2009 09:48:12 +0200 (CEST) 12, 32 -- Message-ID: {4A277DAE.3070404-at-ipcms.u-strasbg.fr} 12, 32 -- Date: Thu, 04 Jun 2009 09:54:22 +0200 12, 32 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 32 -- User-Agent: Thunderbird 2.0.0.21 (X11/20090318) 12, 32 -- MIME-Version: 1.0 12, 32 -- To: RonMervis-at-neurostructural.org, Microscopy-at-microscopy.com 12, 32 -- Subject: Re: [Microscopy] viaWWW: double images in microscope 12, 32 -- References: {200906040137.n541bSU7016129-at-ns.microscopy.com} 12, 32 -- In-Reply-To: {200906040137.n541bSU7016129-at-ns.microscopy.com} 12, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 32 -- Content-Transfer-Encoding: 8bit 12, 32 -- X-IPCMS-MailScanner: Found to be clean 12, 32 -- X-IPCMS-MailScanner-SpamScore: s 12, 32 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 32 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0.1 (mailhost.u-strasbg.fr [130.79.200.154]); Thu, 04 Jun 2009 09:54:40 +0200 (CEST) 12, 32 -- X-Virus-Scanned: ClamAV 0.94.2/9419/Thu Jun 4 04:47:46 2009 on mr4.u-strasbg.fr 12, 32 -- X-Virus-Status: Clean 12, 32 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=USER_IN_WHITELIST 12, 32 -- autolearn=disabled version=3.2.5 12, 32 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr ==============================End of - Headers==============================
Sounds like the interpupillary distance might not be set correctly for this user. The eyepieces are either too far apart or too close together. Try adjusting that until the images converge (without the glasses).
Jeff Stewart Metallographic Laboratory Manager Stern-Leach Company 49 Pearl Street Attleboro, MA 02703 508-222-7400 x1329
On Wed Jun 3 21:29 , RonMervis-at-neurostructural.org sent:
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} -----Original Message----- } From: rana.kasim-at-ist.utl.pt [mailto:rana.kasim-at-ist.utl.pt] } Sent: Wednesday, June 03, 2009 8:30 PM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } This Question/Comment was submitted to the Microscopy } Listserver using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } -------------------------------------------------------------- } ------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both rana.kasim-at-ist.utl.pt as well as the } MIcroscopy Listserver } -------------------------------------------------------------- } ------------- } } Email: rana.kasim-at-ist.utl.pt } Name: Rana Kasim } } Organization: Ph.D student Instituto Superior } TÈcnicoMaterials Engineering Department-university of lisbon } } Title-Subject: [Filtered] Monte carlo simulation with EDS analysis } } Question: Hi All, } Could any person help me to how i can get the Monte carlo } simulation with Energy Dispersive Spectroscopy(EDS)and the } software that can calculate the light volume when transmitt } inside the tissue and its pentration depth. } } Regards } Rana } } Login Host: 193.136.139.253 } -------------------------------------------------------------- } ------------- } } } ==============================Original } Headers============================== } 8, 13 -- From zaluzec-at-microscopy.com Wed Jun 3 20:29:27 2009 } 8, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 8, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id n541TQd4032314 } 8, 13 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jun } 2009 20:29:27 -0500 } 8, 13 -- Mime-Version: 1.0 } 8, 13 -- Message-Id: {p06240802c64cd3d8866b-at-[206.69.208.22]} } 8, 13 -- Date: Wed, 3 Jun 2009 20:29:26 -0500 8, 13 -- To: } microscopy-at-microscopy.com 8, 13 -- From: } rana.kasim-at-ist.utl.pt (by way of MicroscopyListserver) 8, 13 } -- Subject: viaWWW: Monte carlo simulation with EDS analysis } 8, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; } format="flowed" } 8, 13 -- Content-Transfer-Encoding: 8bit 8, 13 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id n541TQd4032314 } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 6, 25 -- From DusevichV-at-umkc.edu Thu Jun 4 08:23:10 2009 6, 25 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n54DN8bU026519 6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Jun 2009 08:23:09 -0500 6, 25 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 25 -- Thu, 4 Jun 2009 08:23:06 -0500 6, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 6, 25 -- Content-class: urn:content-classes:message 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Type: text/plain; 6, 25 -- charset="iso-8859-1" 6, 25 -- Subject: RE: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis 6, 25 -- Date: Thu, 4 Jun 2009 08:23:05 -0500 6, 25 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB867-at-KC-MSX1.kc.umkc.edu} 6, 25 -- In-Reply-To: {200906040129.n541Tusk001241-at-ns.microscopy.com} 6, 25 -- X-MS-Has-Attach: 6, 25 -- X-MS-TNEF-Correlator: 6, 25 -- Thread-Topic: [Microscopy] viaWWW: Monte carlo simulation with EDS analysis 6, 25 -- Thread-Index: Acnks/iFFG6w/RwCQS2xrKe6YDEkxAAY3UTw 6, 25 -- References: {200906040129.n541Tusk001241-at-ns.microscopy.com} 6, 25 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 6, 25 -- To: {rana.kasim-at-ist.utl.pt} , {Microscopy-at-microscopy.com} 6, 25 -- X-OriginalArrivalTime: 04 Jun 2009 13:23:06.0297 (UTC) FILETIME=[988A2A90:01C9E517] 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n54DN8bU026519 ==============================End of - Headers==============================
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Email: richard.ross-at-allisontransmission.com Name: Richard Ross
Organization: Allison Transmission Inc.
Title-Subject: [Microscopy] viaWWW: double images in microscope
Question: Could this person be one of the 10-15% of the population that does not have binocular vision? I am one of these, and my less-dominant eye is ignored for my central vision, so no microscope issues (other than no stereo 3-d capability!). Perhaps this individual's visual cortex is develeoped somewhere between binocular and monocular vision and has trouble fusing the view presented in a microscope. Just some thoughts to consider.
Is it possible, for the commonly used resins in TEM embedding (like Epon, Durcupan / Araldite, ERL, LR White or Gold, Lowi's, etc), to give a figure for their relative hardness on the Mohs hardness scale? relative to Copper and Gold, e.g. ? Does anybody know a source for this type of information? best regards, Reinhard
--
PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
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I have 'double vision', although this has been minimised with corrective surgery a few years ago [my 'bad' right eye had drifted away from that of my childhood and my brain was starting to process the visual info again from it - so they put the eye pointing inwards again to switch it 'off', as the brain was used to ignoring it there].
All this was from a lazy eye [squint] at childhood though and a clear visual defect. In fact I keep the microscope binoculars open too wide so I only look down with the left [good] eye* - I shift the good eye from right to left binocular during cleaning for the users. However my lazy eye really counts as a major visual problem and apparently your user hasn't got anything like that [my right eyes perfect just the brain doesn't process the info as it didn't like the double vision either when growing during my youth]. Visual/cortex problems can produce odd effects - there's one where people think they see little grey people...
I expect straining to peer down the microscope is knocking the eye out of 3D alignment [you can make the eyes squint inwards if you try - personally I don't], but perhaps someone from optimetrics can advise. Try the monocular approach as well.
Keith
*I don't suppose many remember the monocular microscopes of yore, except perhaps from school, but there's no stereoscopic info on a compound microscope so monocular is fine. Not that I'd see 3D information anyway - in fact we make good photographers, probably because we have an eye for the 2D scene.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: richard.ross-at-allisontransmission.com [mailto:richard.ross-at-allisontransmission.com] Sent: 05 June 2009 00:31 To: kjmorris-at-well.ox.ac.uk
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Email: richard.ross-at-allisontransmission.com Name: Richard Ross
Organization: Allison Transmission Inc.
Title-Subject: [Microscopy] viaWWW: double images in microscope
Question: Could this person be one of the 10-15% of the population that does not have binocular vision? I am one of these, and my less-dominant eye is ignored for my central vision, so no microscope issues (other than no stereo 3-d capability!). Perhaps this individual's visual cortex is develeoped somewhere between binocular and monocular vision and has trouble fusing the view presented in a microscope. Just some thoughts to consider.
it seems to me that there weren't many replies to your question...as far as from replies via the MSA Listserver.
I would like to add a reference for an article, published most recently on this topic:
Cf. SAWAGUCHI A, AOYAMA F, IDE S & SUGANUMA T: Capsule-Supporting ring: a new device for resin embedding of glass-mounted specimens. J. Micr. 234 pt 2(no. 849),pp. 113-117, 2009
Special reference to: - Re-embedding of epoxy-resin semithin sections for morphological observation, - Re-embedding of Lowicryl K4M semithin sections for immunocytochemical observation - in situ flat embedding of cultured cells on glass cover slip
You'll find a very sophisticated technique, sufficiently illustrated, to reembed distinct and small areas from a thick (semithin) resin section for a subsequent (T)EM-observation.
If you can't retrieve the paper from the Journal I could help you with a pdf of the article.
Best wishes and good luck, have a nice weekend,
Wolfgang MUSS EM-Lab/Pathology SALK-LKH (Gen.Hosp.) - PMU (Priv. Paracelsus Med. Univ.) SALZBURG AUSTRIA-Europe
} -----Ursprüngliche Nachricht----- } Von: hong_s-at-palmer.edu [mailto:hong_s-at-palmer.edu] } Gesendet: Mittwoch, 27. Mai 2009 21:33 } An: Muß Wolfgang } Betreff: [Microscopy] Reembedment of a semithin section } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both hong_s-at-palmer.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } Email: hong_s-at-palmer.edu } Name: Se Pyo Hong } Organization: Graduate Studies at Palmer College of Chiropractic } Title-Subject: reembedment of a semithin section } Question: } } } Hi everyone; } I have 2-micron thick flat specimens (cross sectioned rat } spinal cord) embedded in epon with toluidine stain on a glass } slide. I want to have ultrathin sections of part of the } specimen (Raxed lamina 1 - 3 area). } Please give me suggestions or a procedure. Thank you.. } } } Login Host: 198.102.161.2 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Wed May 27 14:28:30 2009 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n4RJSTj8019343 } 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2009 14:28:29 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c64344cbf431-at-[206.69.208.22]} } 7, 11 -- Date: Wed, 27 May 2009 14:28:27 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: hong_s-at-palmer.edu (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: reembedment of a semithin section } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of Headers============================== }
==============================Original Headers============================== 17, 36 -- From W.Muss-at-salk.at Fri Jun 5 08:29:55 2009 17, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 17, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55DTqJf015595 17, 36 -- for {microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 08:29:54 -0500 17, 36 -- Received: from localhost (localhost [127.0.0.1]) 17, 36 -- by hermes.salk.at (Postfix) with ESMTP id 55D69C3856; 17, 36 -- Fri, 5 Jun 2009 15:29:47 +0200 (CEST) 17, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 17, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 17, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 17, 36 -- with ESMTP id 5-e4JbdQRJvI; Fri, 5 Jun 2009 15:29:46 +0200 (CEST) 17, 36 -- Received: from n2rz123.lksdom21.lks.local (n2rz123.lksdom21.lks.local [192.168.101.123]) 17, 36 -- by hermes.salk.at (Postfix) with ESMTP id DD3A6C3875; 17, 36 -- Fri, 5 Jun 2009 15:29:46 +0200 (CEST) 17, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 17, 36 -- Fri, 5 Jun 2009 15:29:47 +0200 17, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 36 -- Content-class: urn:content-classes:message 17, 36 -- MIME-Version: 1.0 17, 36 -- Content-Type: text/plain; 17, 36 -- charset="iso-8859-1" 17, 36 -- Subject: [Microscopy] Re: Reembedment of a semithin section 17, 36 -- Date: Fri, 5 Jun 2009 15:29:46 +0200 17, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062938715-at-N1RZ116.lksdom21.lks.local} 17, 36 -- In-Reply-To: {200905271933.n4RJXSkk030084-at-ns.microscopy.com} 17, 36 -- X-MS-Has-Attach: 17, 36 -- X-MS-TNEF-Correlator: 17, 36 -- Thread-Topic: [Microscopy] Re: Reembedment of a semithin section 17, 36 -- Thread-Index: AcnfAgfphaNX5HVORKOAASO2yByKxAG3eyOQ 17, 36 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 17, 36 -- To: {hong_s-at-palmer.edu} 17, 36 -- Cc: {microscopy-at-microscopy.com} 17, 36 -- X-OriginalArrivalTime: 05 Jun 2009 13:29:47.0087 (UTC) FILETIME=[B1D79DF0:01C9E5E1] 17, 36 -- X-Scanned-By: SALK-Content-Filter 17, 36 -- Content-Transfer-Encoding: 8bit 17, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id n55DTqJf015595 ==============================End of - Headers==============================
Ron, I always had double vision when using binocular eyepieces on binoculars as a child and through college, and never needed or wore glasses. For my first job in EM after college, I still had double-vision when using binocular microscopes. After 3-5 months of sectioning on the ultramicrotome and favoring one eye, I was looking at a semithin section on a basic binocular compound microscope one day, and the two images slowly started moving together until they overlapped. I didn't move at all during this 1-2 second experience. (It probably only seemed that long, though.) I have been able to use binocular microscopes correctly ever since.
My theory is that my brain just needed to adjust to this uncommon phenomenon of binocular eyepieces, and merely took longer than the average person.
As a follow-up: When I got eyeglasses a few years afterwards, I had a terrible time adjusting to every new prescription for several years. Now the adjustment is very brief.
Good luck to your student. Advise them to stick with it, and it may correct itself.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
"We are all individuals!" -- Monty Python, The Life of Brian. (The quote seems appropriate here.)
-----Original Message----- X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk] Sent: Friday, June 05, 2009 5:46 AM To: Sobocinski, Gregg
I have 'double vision', although this has been minimised with corrective surgery a few years ago [my 'bad' right eye had drifted away from that of my childhood and my brain was starting to process the visual info again from it - so they put the eye pointing inwards again to switch it 'off', as the brain was used to ignoring it there].
All this was from a lazy eye [squint] at childhood though and a clear visual defect. In fact I keep the microscope binoculars open too wide so I only look down with the left [good] eye* - I shift the good eye from right to left binocular during cleaning for the users. However my lazy eye really counts as a major visual problem and apparently your user hasn't got anything like that [my right eyes perfect just the brain doesn't process the info as it didn't like the double vision either when growing during my youth]. Visual/cortex problems can produce odd effects - there's one where people think they see little grey people...
I expect straining to peer down the microscope is knocking the eye out of 3D alignment [you can make the eyes squint inwards if you try - personally I don't], but perhaps someone from optimetrics can advise. Try the monocular approach as well.
Keith
*I don't suppose many remember the monocular microscopes of yore, except perhaps from school, but there's no stereoscopic info on a compound microscope so monocular is fine. Not that I'd see 3D information anyway - in fact we make good photographers, probably because we have an eye for the 2D scene.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: richard.ross-at-allisontransmission.com [mailto:richard.ross-at-allisontransmission.com] Sent: 05 June 2009 00:31 To: kjmorris-at-well.ox.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both richard.ross-at-allisontransmission.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: richard.ross-at-allisontransmission.com Name: Richard Ross
Organization: Allison Transmission Inc.
Title-Subject: [Microscopy] viaWWW: double images in microscope
Question: Could this person be one of the 10-15% of the population that does not have binocular vision? I am one of these, and my less-dominant eye is ignored for my central vision, so no microscope issues (other than no stereo 3-d capability!). Perhaps this individual's visual cortex is develeoped somewhere between binocular and monocular vision and has trouble fusing the view presented in a microscope. Just some thoughts to consider.
I guess this topic has been discussed in this forum (I found that the searching function in ListServer achieves was not so straightforward, spoiled by Google), but I also think that there should be some newer system(s)/technique available.
We have a 25yr-old Philips SEM 505 and are interested in upgrading it to digital photography capability. I am looking advice for the system should be considered. And estimated cost including computer and software (preferably cheaper)?
In addition, does anyone still keep some Polaroid 665 films that we can buy before ours run out?
Thank you in advance!
Guosheng Liu Dept of Biology, Univ of Saskatchewan Canada
==============================Original Headers============================== 7, 20 -- From gul417-at-mail.usask.ca Fri Jun 5 10:42:37 2009 7, 20 -- Received: from smtp.usask.ca (smtp.usask.ca [128.233.192.40]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id n55FgaHK016599 7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Jun 2009 10:42:37 -0500 7, 20 -- Received: from conversion-daemon.usask.ca by usask.ca 7, 20 -- (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007)) 7, 20 -- id {0KKR00601VHZX6-at-usask.ca} (original mail from gul417-at-mail.usask.ca) 7, 20 -- for Microscopy-at-microscopy.com; Fri, 05 Jun 2009 09:42:36 -0600 (CST) 7, 20 -- Received: from paws3 (paws3.usask.ca [128.233.194.31]) 7, 20 -- by usask.ca (iPlanet Messaging Server 5.2 HotFix 2.18 (built Jul 3 2007)) 7, 20 -- with SMTP id {0KKR00IV7VN09I-at-usask.ca} for Microscopy-at-microscopy.com; Fri, 7, 20 -- 05 Jun 2009 09:42:36 -0600 (CST) 7, 20 -- Date: Fri, 05 Jun 2009 09:42:35 -0600 (CST) 7, 20 -- From: Guosheng Liu {gul417-at-mail.usask.ca} 7, 20 -- Subject: SEM Philips505--Polaroid films and upgrade to digital 7, 20 -- To: Microscopy-at-microscopy.com 7, 20 -- Message-id: {6016978.1244216555923.JavaMail.gul417-at-mail.usask.ca} 7, 20 -- MIME-version: 1.0 7, 20 -- Content-type: text/plain; charset=UTF-8 7, 20 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
Dear Guosheng, if you are able to do some soldering in the scan generator of the 505 there is a not too costly possibility to do a slow scan frame grabbing up to two channels with an USB A/D converter, using a programm a friend of mine wrote for his 505. In this case please reply to me offline. Then there is - and this I use for my work - to use anexternal digital scan generator and an external framegrabber. I use DISS5 from www.pointelectronic.de/english/products/diss5_english.htm With software this solution starts at ca. 8500 Euros, going up with eds support and 4 channel grabbing to ca. 13 000 Euros. No adverdisement, just a satisfied customer :-)
Best wishes, Stefan
gul417-at-mail.usask.ca schrieb: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } I guess this topic has been discussed in this forum (I found that the } searching function in ListServer achieves was not so straightforward, } spoiled by Google), but I also think that there should be some newer } system(s)/technique available. } } We have a 25yr-old Philips SEM 505 and are interested in upgrading it } to digital photography capability. I am looking advice for the system } should be considered. And estimated cost including computer and } software (preferably cheaper)? } } In addition, does anyone still keep some Polaroid 665 films that we can } buy before ours run out? } } Thank you in advance! }