James, Have you looked at "operating microscopes"? Although binocular (stereo), they generally have very long working distances. I have an old Zeiss dissecting scope with a focal length in the range of 200mm. I love it, although there are certainly issues on resolution at 40X. I mostly use it at 6X.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: james99-at-uab.edu [mailto:james99-at-uab.edu] Sent: Monday, December 31, 2007 11:47 AM To: kenconverse-at-qualityimages.biz
This Question was submitted to Ask-A-Microscopist by (james99-at-uab.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 19, 2007 at 13:52:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both james99-at-uab.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: james99-at-uab.edu Name: James Borham
Organization: University of Alabama at Birmingham
Education: Undergraduate College
Location: Birmingham, Alabama, United States of America
Title: Finding a Specialized Microscope
Question: I'm trying to find a new microscope with some specialized features, and I'm having a lot of trouble. I'm hoping one of you may be able to help me out. The feature that is needed most, and has been impossible to find, is a non-stereo microscope and/or lens with a parfocalizing distance of 90 mm or more. Do you have any idea where I could find such a thing? Is a device with such a long parfocalizing distance even classified as a microscope? I would appreciate any help on this problem.
==============================Original Headers============================== 8, 31 -- From zaluzec-at-microscopy.com Mon Dec 31 10:43:47 2007 8, 31 -- Received: from cubert.e-centre.net (morbo.e-centre.net [66.154.82.3]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBVGhlg5000383 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 10:43:47 -0600 8, 31 -- Received: from [10.3.1.19] (helo=barracuda2.stayonline.net) 8, 31 -- by cubert.e-centre.net with esmtp (Exim 4.50) 8, 31 -- id 1J9Njt-0003Pf-OC 8, 31 -- for microscopy-at-microscopy.com; Mon, 31 Dec 2007 11:43:46 -0500 8, 31 -- X-ASG-Debug-ID: 1199119423-31474-157-0 8, 31 -- X-Barracuda-URL: http://10.3.1.19:8000/cgi-bin/mark.cgi 8, 31 -- Received: from et-lax-21.site.stayonline.net (unknown [74.8.222.131]) 8, 31 -- by barracuda2.stayonline.net (Spam Firewall) with ESMTP id 7472B198715F 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 11:43:43 -0500 (EST) 8, 31 -- Received: from [172.16.7.135] ([172.16.7.135]) 8, 31 -- by et-lax-21.site.stayonline.net (8.13.8/8.12.6) with ESMTP id lBVGhgsN025334 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 16:43:42 GMT 8, 31 -- Mime-Version: 1.0 8, 31 -- Message-Id: {p06240805c39ecc1cefb9-at-[172.16.7.135]} 8, 31 -- Date: Mon, 31 Dec 2007 10:41:26 -0600 8, 31 -- To: microscopy-at-microscopy.com 8, 31 -- From: james99-at-uab.edu (by way of MicroscopyListserver) 8, 31 -- X-ASG-Orig-Subj: AskAMicroscopist: Finding a Specialized Microscope 8, 31 --
Analytical Scientist/Electron Microscopist
The University of Wisconsin Eau Claire is seeking applicants for an Analytical Scientist/Electron Microscopist in the Materials Science Center, an interdisciplinary analytical facility, specializing in materials characterization. This is a full-time professional academic staff position beginning as early as July 1st 2008. Potential applicants may obtain a complete position description and application requirements at www.uwec.edu/Matsci/EMposition.html. Women, minorities, individuals with disabilities and veterans are encouraged to apply. The University is responsive to the needs of dual career couples. Criminal background checks are required prior to employment.
------------------------------------------------------------------------ Dr. Doug Dunham Director, Materials Science Center University of Wisconsin Eau Claire 105 Garfield Avenue Eau Claire, WI 54701 715-836-5312 fax: 715-836-3955 dunhamdj-at-uwec.edu
==============================Original Headers============================== 9, 25 -- From DUNHAMDJ-at-uwec.edu Wed Jan 2 11:09:00 2008 9, 25 -- Received: from exch07.uwec.edu (exch07.uwec.edu [137.28.1.180]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m02H8xqc001540 9, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jan 2008 11:08:59 -0600 9, 25 -- Received: from exch06.uwec.edu (137.28.1.171) by exch07.uwec.edu 9, 25 -- (137.28.1.180) with Microsoft SMTP Server (TLS) id 8.1.240.5; Wed, 2 Jan 2008 9, 25 -- 11:08:59 -0600 9, 25 -- Received: from CHERRYCOKE.uwec.edu ([172.28.1.61]) by exch06.uwec.edu 9, 25 -- ([137.28.1.171]) with mapi; Wed, 2 Jan 2008 11:08:59 -0600 9, 25 -- From: "Dunham, Douglas J." {DUNHAMDJ-at-uwec.edu} 9, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 25 -- Date: Wed, 2 Jan 2008 11:08:58 -0600 9, 25 -- Subject: Electron Microscopist Position 9, 25 -- Thread-Topic: Electron Microscopist Position 9, 25 -- Thread-Index: AchNYinSUpKjC6dCQF2sw1zt9VpmMA== 9, 25 -- Message-ID: {EEA4CA65D05DC54E874A89D9F51868193CC436717A-at-CHERRYCOKE.uwec.edu} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- acceptlanguage: en-US 9, 25 -- Content-Type: text/plain; charset="us-ascii" 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m02H8xqc001540 ==============================End of - Headers==============================
This looks just like a system I saw at Harvard in May 1994 for imaging lung with asbestos or other fibers for 3D reconstruction using VoxelView running on an SGI.
-mc
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 7, 30 -- From cammer-at-aecom.yu.edu Wed Jan 2 13:48:24 2008 7, 30 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m02JmOa9017427 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jan 2008 13:48:24 -0600 7, 30 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 7, 30 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 8C5F69F0346; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 7, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 69C3E8B4006; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- X-AuditID: 816201a0-a80bdbb000006615-ab-477bea88354d 7, 30 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 7, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 2AAAD718002; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 7, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 30 -- (No client certificate requested) 7, 30 -- by post.aecom.yu.edu (Postfix) with ESMTP id 20F1C25; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- Message-Id: {7.0.1.0.2.20080102144518.02309670-at-aecom.yu.edu} 7, 30 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 7, 30 -- Date: Wed, 02 Jan 2008 14:48:08 -0500 7, 30 -- To: takenomm-at-u.washington.edu, microscopy-at-microscopy.com 7, 30 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 7, 30 -- Subject: Re: [Microscopy] Re: MRI ? on maize embryos 7, 30 -- In-Reply-To: {200712282032.lBSKWJou027643-at-ns.microscopy.com} 7, 30 -- References: {200712282032.lBSKWJou027643-at-ns.microscopy.com} 7, 30 -- Mime-Version: 1.0 7, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 30 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
Here is the January 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Tuesday January 8, 2008.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor ======================== Microscopy Used to Discover New, Cool Mineral! Stephen W. Carmichael1, Mayo Clinic
Material Contrast of Scanning Electron and Ion Microscope Images of Metals T. Suzukia*, M. Kudo*, Y.Sakai*, and T. Ichinokawa**, *JEOL Ltd., Akishima, Tokyo, **Waseda University, Tokyo, Japan
Effective Cell Identification and Segmentation in Fluorescence Microscopy with New Fluorescent Whole Cell Stains Suk J. Hong, Richik N. Ghosh, Thermo Fisher Scientific Inc., Rockford, IL
Giving your SEM or FIB a Helping Hand Neil Rowlands, Oxford Instruments, Concord, USA, Gavin Frayne, Kleindiek Nanotechnik, Tubingen, Germany, Bo Svarrer Hansen, Capres A/S, Lyngby, Denmark
An Introduction to 3D Microscopy Techniques Megan MacNeil and Duncan McMillan, Carl Zeiss MicroImaging, Inc. Thornwood, NY.
Scanning Transmission Electron Microscopy for Critical Dimension Monitoring in Wafer Manufacturing Haifeng Wang*, Jason Fang*, Jason Arjavac**,Rudy Kellner**, *Western Digital Corporation, Fremont, CA, **FEI Company, Hillsboro, OR
Nanoelectromechanics of Inorganic and Biological Systems: From Structural Imaging to Local Functionalities B. J. Rodriguez,1,2 S. V. Kalinin,1,2 S. Jesse,1 G. Thompson,3 A. Vertegel,3 S. Hohlbauch,4 R. Proksch4 ,1Mat. Sci. & Tech, and 2Ctr. for Nanophase Matl. ORNL, Oak Ridge, TN, 3 Clemson University, SC, 4Asylum Research, Santa Barbara, CA
Spatial Resolution in ACOM–What Will Come After EBSD R.A. Schwarzer, Kappstr. 65, D-71083 Herrenberg, Germany
Lab-Tek Chamber Slide for TEM Prep: A Simple, Rapid, and Reliable Protocol for In Situ Embedding Monolayer Cell Cultures in Epoxy and LR White Resin Gang Ning, Penn State University, State College, PA
Imaging Carbon Nanoparticles in Cells Mhairi Gass* & Alexandra Porter**, *SuperSTEM, Daresbury Lab., Daresbury, **Imperial College London, London, U.K.
Automatic Acquisition and Image Analysis of 2D Crystals N. Coudray*, F. Beck**, J.-L. Buessler*, A. Korinek**, A. Karathanou*, H. Rémigy***, H. Kihl*, A. Engel***, J.M. Plitzko**, J.-P. Urban*, *Université de Haute Alsace, Mulhouse, France, **Max Planck Inst. Martinsried, Germany, ***University of Basel, Switzerland
Industry News
NetNotes SAMPLE PREPARATION - osmium ignition SAMPLE PREPARATION – wood for TEM SAMPLE PREPARATION - perchloric acid hazards SAMPLE PREPARATION - differential polymer staining MICROTOMY - alternative ultramicrotome knives IMAGE ANALYSIS - stitching high-resolution microscope images IMMUNOCYTOCHEMISTRY – adherent cells IMMUNOCYTOCHEMISTRY - pre-embedding tissue cultures TEM: Objective aperture vs. EFTEM TEM – alignment problem TEM - lattice fringes TEM - micelle solutions TEM - ice contamination EDX - plants and seeds EDX - biological sample
Dear Abbe
Advertiser's Index
==============================Original Headers============================== 19, 18 -- From randerson20-at-tampabay.rr.com Wed Jan 2 15:01:45 2008 19, 18 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 19, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m02L1gmZ031225 19, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Jan 2008 15:01:43 -0600 19, 18 -- Received: from [127.0.0.1] (really [24.73.73.214]) 19, 18 -- by hrndva-omta03.mail.rr.com with ESMTP 19, 18 -- id {20080102210140.KPHF2900.hrndva-omta03.mail.rr.com-at-[127.0.0.1]} ; 19, 18 -- Wed, 2 Jan 2008 21:01:40 +0000 19, 18 -- Message-ID: {477BFBB0.5070109-at-tampabay.rr.com} 19, 18 -- Date: Wed, 02 Jan 2008 16:01:36 -0500 19, 18 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 19, 18 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 19, 18 -- MIME-Version: 1.0 19, 18 -- To: Listserver {Microscopy-at-Microscopy.Com} , 19, 18 -- Confocal Microscopy List {CONFOCAL-at-LISTSERV.BUFFALO.EDU} 19, 18 -- Subject: January 2008 Microscopy Today Table of Contents 19, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Has anyone embedded pine needles in JB-4 and sectioned for LM? I had problem with orientation when I used plastic beem capsules before. I have been trying silicon rubber molds under 10psi. But it doesn't polymerize competely. What is the max psi i can go not to damage both tissue and plastic. I have been also thinking of switching to LR-white. But I dont know if i get better result. I would appreciate any kind of advice on this.
Thanks.
Sau Silwal
Send instant messages to your online friends http://uk.messenger.yahoo.com
==============================Original Headers============================== 7, 19 -- From sau_silwal-at-yahoo.com Wed Jan 2 15:16:11 2008 7, 19 -- Received: from web30112.mail.mud.yahoo.com (web30112.mail.mud.yahoo.com [209.191.69.44]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m02LGB8F011221 7, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Jan 2008 15:16:11 -0600 7, 19 -- Received: (qmail 58393 invoked by uid 60001); 2 Jan 2008 21:16:10 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 19 -- b=OtgCzeGTpLV1pbFgc3Y11EJS0De4rrMzsoukd0VL3Ba+tqHRFzXqiEDkddOqKTuX3DlYbx5KhHtN+j8mT4QEArNfI5neC1lVxErBFcPaO5ujyYp32hxRMMVUQhO6a0dtwyez40q6rqVqolb5jRrBH0o5GJcj44y78ZnXcgMqhHw=; 7, 19 -- X-YMail-OSG: k1zyU3wVM1le9chAoqIW7gcRZBdH5fik_w0y5g286myMXe9z5iWTOf9UY07vpCxeUCJJja.zbDZT4_naZRIDdU4ltAwA1J4fXq8lzsq4W.pnp55Gt2jxxIiJOGXuyg-- 7, 19 -- Received: from [65.188.180.191] by web30112.mail.mud.yahoo.com via HTTP; Wed, 02 Jan 2008 21:16:10 GMT 7, 19 -- Date: Wed, 2 Jan 2008 21:16:10 +0000 (GMT) 7, 19 -- From: Saubhagya Silwal {sau_silwal-at-yahoo.com} 7, 19 -- Subject: Re: Embedding pine needles in JB-4 7, 19 -- To: Microscopy-at-Microscopy.Com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- Message-ID: {757701.58226.qm-at-web30112.mail.mud.yahoo.com} ==============================End of - Headers==============================
Has anyone embedded pine needles in JB-4 and sectioned for LM? I had problem with orientation when I used plastic beem capsules before. I have been trying silicon rubber molds under 10psi. But it doesn't polymerize competely. What is the max psi i can go not to damage both tissue and plastic. I have been also thinking of switching to LR-white. But I dont know if i get better result. I would appreciate any kind of advice on this.
Thanks.
Sau Silwal
Send instant messages to your online friends http://uk.messenger.yahoo.com
==============================Original Headers============================== 7, 19 -- From sau_silwal-at-yahoo.com Wed Jan 2 15:16:51 2008 7, 19 -- Received: from web30104.mail.mud.yahoo.com (web30104.mail.mud.yahoo.com [209.191.69.36]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m02LGmYY012542 7, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Jan 2008 15:16:51 -0600 7, 19 -- Received: (qmail 90828 invoked by uid 60001); 2 Jan 2008 21:16:48 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 19 -- b=T68d+yta5cWOzfibtEsYoAkJNlZ8jr7ckxVfvtrsAyzuJCy2WtA2Xgw5px2NrMBAAEK/Q8UaE9taqy8zSNuw+t3x3102mEgxwvkMB15fuRvMKquO+iL/A+HkYKH7zGdKTEjwWGm5iJhiu8NiF+8vOVtF/MgbyBWSFK/AoOao9WE=; 7, 19 -- X-YMail-OSG: CG53fXUVM1nltvLPOODYEEh5AffxIXkERFL5lqXHzwzlPPLehI7pK7gYOYMEYo2ytw-- 7, 19 -- Received: from [65.188.180.191] by web30104.mail.mud.yahoo.com via HTTP; Wed, 02 Jan 2008 21:16:46 GMT 7, 19 -- Date: Wed, 2 Jan 2008 21:16:46 +0000 (GMT) 7, 19 -- From: Saubhagya Silwal {sau_silwal-at-yahoo.com} 7, 19 -- Subject: Re: Embedding pine needles in JB-4 7, 19 -- To: Microscopy-at-Microscopy.Com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- Message-ID: {902929.90487.qm-at-web30104.mail.mud.yahoo.com} ==============================End of - Headers==============================
Sau, Not sure exactly if this will solve your problem, but for what its worth, you can get capsules that are like BEEMs except that they have a completely flat bottom. They are made by TAAB but available from major supply houses (no finanical connection). This gives a nice 7 mm (or so) flat surface. You can also get this out of a standard BEEM by flipping it upside down, although you have to mess about to keep the resin from leaking (definitely a mess, but possible to do).
Hope this helps, Tobias
} } Hi, } } Happy New Year!! } } Has anyone embedded pine needles in JB-4 and sectioned } for LM? I had problem with orientation when I used } plastic beem capsules before. I have been trying } silicon rubber molds under 10psi. But it doesn't } polymerize competely. What is the max psi i can go not } to damage both tissue and plastic. } I have been also thinking of switching to LR-white. } But I dont know if i get better result. I would } appreciate any kind of advice on this. } } Thanks. } } Sau Silwal }
Sorry for the late reply, but you might contact Jamie Weichert at UW-Madison: http://www.radiology.wisc.edu/research/contrastAgentLab/index.php
He works on this sort of problem for both MRI and microCT. The issue is more likely, can the instruments get the resolution you need? But I suspect they can.
Phil
} } Daar coleagues } I am working on a project on the vascular puncture inoculation } (VPI) of maize viruses into maize embyos. We need to have a 3-D } picture of the vascular bundles in (germinating) maize embyos, and a } colleage of mine suggested MRI as a possible means to achieve that. } I wonder if anyone has tried MRI on plants/ plant tissues, or if } someone can suggest another more suitable method (apart from } LM-serial sectioning). Thanks and Happy New Year to everyone. } } } El-Desouky Ammar, Ph.D. } Dept. of Entomology, OSU, } 019 Selby Hall, } OARDC, Wooster, OH 44691 } Tel: 330-263-3830. } FAX: 330-202-3563. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 22 -- From oshel1pe-at-cmich.edu Thu Jan 3 09:00:55 2008 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03F0tDU006319 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 09:00:55 -0600 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m03FIA9N005799 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 10:18:10 -0500 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 22 -- Thu, 3 Jan 2008 10:00:05 -0500 4, 22 -- Mime-Version: 1.0 4, 22 -- Message-Id: {f06240808c3a2a83b8dca-at-[141.209.160.249]} 4, 22 -- In-Reply-To: {200712281726.lBSHQR4i001693-at-ns.microscopy.com} 4, 22 -- References: {200712281726.lBSHQR4i001693-at-ns.microscopy.com} 4, 22 -- Date: Thu, 3 Jan 2008 10:00:48 -0500 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 22 -- Subject: Re: [Microscopy] MRI ? on maize embryos 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 22 -- X-OriginalArrivalTime: 03 Jan 2008 15:00:05.0856 (UTC) FILETIME=[5349E600:01C84E19] 4, 22 -- X-CanItPRO-Stream: default 4, 22 -- X-Spam-Score: -4 () L_EXCH_MF 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
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I am an amateur naturalist. I have made by myself a microtome and I also use to honing the blade with results quite good. Now I am testing a disposable blade. It was a kindly gift of a friend of mine who works in an histology laboratory. I wonder where could I find disposable blades because it seems they are working well . Otherwise it is quite difficult to make and honing a blade by myself. Does anybody know a factory that produces such kind of blades? Any suggestions would be greatly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jill.Verlander-at-medicine.ufl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
We returned to work today after a hiatus since 12/21. The afternoon before we shut down the lab for Christmas break, we turned off the scopes and chillers. All was well with the equipment before that.
Today, we tried to get everything going again, but the old Coolwell chiller (model S-075W) on our Zeiss EM10 is not working properly. When we tried to run it, there was no flow and the HiTemp warning light on the front panel was lit. This is supposed to shut off the system if the temp rises above 93 degrees F, which it is not (room temperature is 73 degrees, coolant temp about 71 degrees F). However, the "reset" switch on the P6 high temperature safety control box had not popped out.
If you turn the unit on and press the button (S-3) that bridges the high temperature limit control, and have the unit pumping only on itself (a short loop from supply to return), the pump runs normally. Intermittently, it will run normally either on "bypass" (which does not allow the compressor to come on) or on "normal" function. The compressor will come on when the unit is working and set on "normal." However, the unit will spontaneously shut down, and when it does, the hi temperature light comes on.
The compressor is cooled by house tap water: the supply and return lines are open and flowing. The coolant tank is full, magnetic float switch floating, and the filter looks okay - not pristine, but only slightly gray. We did have a "heat event" according to an unrelated computer that was left on during the break, but I don't see how that could have affected the chiller, since it was shut off at the time.
Does anybody have any suggestions as to what might be causing this problem and how to correct it?
Many thanks, and Happy New Year to all!
Jill Verlander Reed University of Florida College of Medicine Electron Microscopy Core Facility
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Email: jeff-at-tss-consulting.com Name: Jeff West
Organization: TSS Consulting Ltd.
Title-Subject: [Filtered] Employment Opportunity
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A minimum of 3 years experience with TEM sample prep and imaging is required. Preference is for candidates with Materials Science or Engineering degrees (BS or MS).
For further information and to apply for this position please contact: Jeff West TSS Consulting, Ltd. (877) 489-2425 Resumes may be sent to: jeff-at-tss-consulting.com
This Question was submitted to Ask-A-Microscopist by (susan.trant-at-viha.ca) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, December 31, 2007 at 15:28:34 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both susan.trant-at-viha.ca as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: susan.trant-at-viha.ca Name: Susan Trant
Education: Graduate College
Location: Victoria, British Columbia, Canada
Title: EM film
Question: Hello everyone
I purchased Eastman Fine Grain Release Positive Film 5302 from Electron Microscopy Sciences for my TEM. The film is much thicker than the current product that I am using. I contacted some photo experts and they said that I needed a product called Dektol for a film developer.(Kodak) Does anyone use this film and if so, what developer do you use and what temperature and length of time for processing? The photography shop that I talked to also said that you do not dilute the stock solution before use. We are receiving a new digital imaging system within the year.
Thanks
Sue Trant EM Technologist Vancouver Island Health Authority Victoria, British Columbia, Canada
I trashed my files on this film about six months ago thinking that I'd never need to consult it again. There are several things that I do remember that may be of interest to you. The following is from a 25 year old memory!
This film is quite good for TEM and the sheet film is thick also. You will need to do an exposure test to see what gives the best results. If you have an older scope you should be able to change the exposure time. Do several times of the same area.
Keep film away from light! I used a very dark red filtered light that was so dark that it was nearly useless even after giving my eyes time to adjust. To load the film both into the scope camera holder and into the developing tank I would turn my back to the light and load in front of me.
Dektol seems correct as the developer. I do not remember diluting it - try using it straight from the stock solution that you make from the powder. Adjust the temperature before turning out the room light. Try hot water around a beaker with the developer in it and stir gently with the thermometer. I used a brown gal. bottle to store the developer, kept in the darkroom for a month or so with no problems as to age. Just make sure the bottle is stoppered well. If the developer starts to turn brown it is time to make up a new batch. I think the temperature for making the developer is much hotter than the film developing temperature so make sure you make it up with enough time to cool before you want to use it. (Stoppers work better than caps in my opinion)
Four minute development stands out in my mind; agitated several times. I used the old apron type from Kodak to do the loading but any standard 35 mm developing tank should work well. Make positive that you do not have any bubbles stuck to the film by banging the tank on a counter surface a few times after the developer is in the tank with the film. Water rinse twice. Kodak Rapid fixer 4 min. with agitation several times Wash in running for 30 min. Photoflo rinse for 15 sec. Hang to dry with a weight on the bottom (to eliminate curling)
I used the film without perforations (holes along the sides). This increased the area of the image greatly. If you do have a camera that can use this film and have a negative holder that you can spare, find a shop that can make the viewing holes of the holder larger by a few mm. If scanning into a computer there will be no problem.
Hoping my recollections are accurate, Pat
Patricia Stranen Connelly NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
} ---------------------------------------------------------------------------- } Email: susan.trant-at-viha.ca } Name: Susan Trant } } Education: Graduate College } Location: Victoria, British Columbia, Canada } Title: EM film } } I purchased Eastman Fine Grain Release Positive Film 5302 from Electron } Microscopy Sciences for my TEM. The film is much thicker than the current } product that I am using. I contacted some photo experts and they said that I } needed a product called Dektol for a film developer.(Kodak) Does anyone use } this film and if so, what developer do you use and what temperature and length } of time for processing? The photography shop that I talked to also said that } you do not dilute the stock solution before use. } We are receiving a new digital imaging system within the year. } } Thanks } } Sue Trant } EM Technologist } Vancouver Island Health Authority } Victoria, British Columbia, } Canada
} ==============================End of - Headers==============================
==============================Original Headers============================== 14, 24 -- From connellyps-at-nhlbi.nih.gov Thu Jan 3 10:41:04 2008 14, 24 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03Gf4Bh003100 14, 24 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 10:41:04 -0600 14, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 14, 24 -- Thu, 3 Jan 2008 11:40:55 -0500 14, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.167]) with Microsoft Exchange Server HTTP-DAV ; 14, 24 -- Thu, 3 Jan 2008 16:40:54 +0000 14, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 14, 24 -- Date: Thu, 03 Jan 2008 11:38:30 -0500 14, 24 -- Subject: Re: [Microscopy] AskAMicroscopist: EM film 5302 14, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 14, 24 -- To: {susan.trant-at-viha.ca} 14, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 24 -- Message-ID: {C3A279B6.12C8%connellyps-at-nhlbi.nih.gov} 14, 24 -- Thread-Topic: [Microscopy] AskAMicroscopist: EM film 5302 14, 24 -- Thread-Index: AchOJxJuUQ2CdroaEdygxgANk2Yv1A== 14, 24 -- In-Reply-To: {200801031512.m03FCW6H008302-at-ns.microscopy.com} 14, 24 -- Mime-version: 1.0 14, 24 -- Content-type: text/plain; 14, 24 -- charset="ISO-8859-1" 14, 24 -- X-OriginalArrivalTime: 03 Jan 2008 16:40:55.0489 (UTC) FILETIME=[6926A310:01C84E27] 14, 24 -- Content-Transfer-Encoding: 8bit 14, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m03Gf4Bh003100 ==============================End of - Headers==============================
You are invited to attend a live, interactive, web-based instructional seminar:
========================================================== "Quantitative Image and Data Acquisition for Fluorescent Specimens" Advice from a Facility Director
Presented by Brian Matsumoto, Ph.D., University of California, Santa Barbara ==========================================================
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When: ===================== Monday, 7-January, 1:30PM (New York time; 10:30 AM California time.) Duration: Approximately 1 hour.
Attendees will learn about issues affecting the quantitative accuracy of fluorescence images acquired through a microscope and will pick up tips and suggestions for improving image quality, making better use of the camera's light collection abilities, and will learn the nomenclature associated with digital imaging. Attendees will leave with a better understanding of how to characterize and optimize their optical system, camera, and software. Bring your questions to this live, interactive web-based seminar.
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About the presenter ===================== Brian Matsumoto, Ph.D. is the Director of the Integrated Microscopy Facility and is an Associate Adjunct Professor for the department of Molecular, Cellular and Developmental Biology Department at the University of California Santa Barbara. He is the author of "Basic Methods in Light Microscopy" (Cambridge University Press) and editor of "Cell Biological Applications of Confocal Microscopy" (Academic Press).
More instructional webinars are also shown at http://magbiosystems.com/education Register for any session of interest and feel encouraged to pass this along to your colleagues.
Sponsored by the Microimaging Applications Group (MAG), a group of independent imaging companies working cooperatively to provide an unparalleled range of solutions for microimaging applications.
This seminar requires that attendees use a Java-enabled browser with a high bandwidth connection. Audio is via toll-free telephone.
There is no charge to participate in this or the other on-line seminars.
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Email: samuel.connell-at-stjude.org Name: Samuel Connell
Organization: St. Jude Childrenís Research Hospital
Title-Subject: [Filtered] Imaging Scientist ñ St. Jude Childrenís Research Hospital
Question: Imaging Scientist ñ St. Jude Childrenís Research Hospital.
Currently, St. Jude Childrenís Research Hospital has an opening for an Imaging Scientist (Job Number 17216) in the Cellular Imaging Department.
The successful candidate would be responsible for assisting faculty, postdoctoral researchers, staff, and collaborators in advance microscopy techniques and methodologies, including laser scanning and spinning disk confocal fluorescence microscopy, wide-field microscopy, live cell imaging, FRET, FLIM, FRAP and TIRF, as well as routine maintenance of the instrumentation infrastructure.
Requirements:
A Bachelor's degree in an appropriate scientific field plus a minimum of ten (10) years (post-degree) of relevant and productive work experience is required OR,
A Master's degree in an appropriate scientific field plus a minimum of nine (9) years (post-degree) of relevant and productive work experience is required OR,
A PhD in an appropriate scientific field plus five (5) years (post-degree) of relevant and productive work experience is required.
To apply please visit our Web site www.stjude.org/jobs
St. Jude Childrenís Research Hospital is an Equal Opportunity Employer.
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Email: mlibbee-at-gmail.com Name: Marissa
Title-Subject: [Filtered] Denton Desk II Maintenance
Question: Greetings! I want to clean up the Denton Desk II sputter coater (equipped with AuPd target) in my lab facility. Does anyone have any suggestions as to how I to clean the plastic encasing? Are there any solvents safe to use for this purpose?
If you are active in or interested in microscopy education, and would like details about an M&M 08 symposium on teaching microscopy and microanalysis, please reply offline by sending your contact information to:
eschumacher-at-mccrone.com
We would like to compile an email address list so that we can send information about the symposium directly to those who would like to receive it. The symposium will cover programs for classroom teaching at all levels, and other methods of training. Please feel free to forward this request to interested colleagues.
Thank you,
Elaine Schumacher, McCrone Associates Charles Lyman, Lehigh University
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Based on your words "disposable" and 'blade', I think you should try disposable Weck Prep Extra Long Blades (2.25 inches long). These are available from various EM supply houses in the US and maybe elsewhere. They do say Weck on them. So check and see if that name is on one side of the blade you got from histology. Some biologists call them surgical blades. So check with your friend to see if that is what he gave you.
In any case, these are single edged "razor blades" designed for surgical cutting or chopping of tissue. Weck Blades are much sharper than regular "razor blades". In my opinion, they are about 3-5 times sharper than ordinary razor blades based on my experience using them to block trim hard Epon epoxy.
Once you start using them, you will stop using regular razor blades and ignore the extra cost.
HTH,
Paul.
At 09:03 AM 1/3/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Plastic encasing? Are you sure? Our Denton Desk II has a glass cylinder, which we regualrly clean (the inside) of simply but carefully running a new (cleaned) razor blade around. Slides on the glass and peels of the metal. We remove the L-seals, and then we use an ethanol soaked cotton coth to remove any remaining bits and finger Prints. The aluminium bits we polish with a "Green-Scratchey-thing" (3M) and more ethanol, then wiped down with toweling.
The only "Plastic" is the teflon bits inside, and again we use ethanol and paper towels or cotton cloth to polish them up. The case work is painted metals and we use general purpose glass/surface cleaner on it.
On 3 Jan 2008 at 14:58, mlibbee-at-gmail.com wrote:
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Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 4 11:57:15 2008 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04HvF9R021636 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Jan 2008 11:57:15 -0600 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04HulEV020683; 10, 25 -- Fri, 4 Jan 2008 12:56:47 -0500 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Hukvd000531; 10, 25 -- Fri, 4 Jan 2008 12:56:46 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: "mlibbee-at-gmail.com" {mlibbee-at-gmail.com} 10, 25 -- Date: Fri, 04 Jan 2008 12:56:46 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] viaWWW: Denton Desk II Maintenance 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {477E2D0E.8384.5D96491-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- References: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
I have a full set of Kidak instructions for 5302. I can scan them and send along if you would like.
Oh, here's Kodak's online:
http://www.kodak.com/US/plugins/acrobat/en/motion/products/lab/h15302. pdf
But to answer:
Safelight: Kodak OA (greenish Yellow) or 1A (the deep dark red mentioned by Patricia Stranen Connelly).
develop:
20C
Dektol (1part dektol : 2 parts water) 2 to 4 mins.
Rinse (water or stopbath)
Fix Kodak fixer 2 to 4 mins
Wash 15 to 20 mins in water or (Hypoclear 1-2 mins then 5 min wash).
I used to regularly use this film for making black and white slides by contact printing B&W Negs. I have never used it for direct EM Exposure. But as far as I know it is NOT a directly invertable film (i.e. it will not give you a positive image after exposing it directly to the beam.) It is not a "35mm slide" type of film (without some fancy developing techniques).
On 3 Jan 2008 at 10:06, susan.trant-at-viha.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by (susan.trant-at-viha.ca) } from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, December 31, 2007 at 15:28:34 } Remember to consider the Grade/Age of the student when considering the Question } --------------------------------------------------------------------------- } Please reply to both susan.trant-at-viha.ca as well as to the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: susan.trant-at-viha.ca } Name: Susan Trant } } Education: Graduate College } } Location: Victoria, British Columbia, Canada } } Title: EM film } } Question: Hello everyone } } I purchased Eastman Fine Grain Release Positive Film 5302 from } Electron Microscopy Sciences for my TEM. The film is much thicker } than the current product that I am using. I contacted some photo } experts and they said that I needed a product called Dektol for a film } developer.(Kodak) Does anyone use this film and if so, what developer } do you use and what temperature and length of time for processing? The } photography shop that I talked to also said that you do not dilute the } stock solution before use. We are receiving a new digital imaging } system within the year. } } Thanks } } Sue Trant } EM Technologist } Vancouver Island Health Authority } Victoria, British Columbia, } Canada } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 11 -- From zaluzec-at-ultra5.microscopy.com Thu Jan 3 09:04:46 2008 } 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03F4iEP015281 } 10, 11 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 09:04:45 -0600 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240806c3a2a9f2f357-at-[206.69.208.22]} } 10, 11 -- Date: Thu, 3 Jan 2008 09:04:43 -0600 } 10, 11 -- To: microscopy-at-microscopy.com } 10, 11 -- From: susan.trant-at-viha.ca (by way of Ask-A-Microscopist) } 10, 11 -- Subject: AskAMicroscopist: EM film 5302 } 10, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 20, 25 -- From edelmare-at-muohio.edu Fri Jan 4 13:39:02 2008 20, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04Jd1PT004770 20, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Jan 2008 13:39:02 -0600 20, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 20, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Jd1aM002755; 20, 25 -- Fri, 4 Jan 2008 14:39:01 -0500 20, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 20, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Jd1rv024391; 20, 25 -- Fri, 4 Jan 2008 14:39:01 -0500 20, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 20, 25 -- To: "susan.trant-at-viha.ca" {susan.trant-at-viha.ca} 20, 25 -- Date: Fri, 04 Jan 2008 14:39:00 -0500 20, 25 -- MIME-Version: 1.0 20, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: EM film 5302 20, 25 -- CC: microscopy-at-Microscopy.com 20, 25 -- Message-ID: {477E4504.31396.636FA6B-at-edelmare.muohio.edu} 20, 25 -- Priority: normal 20, 25 -- In-reply-to: {200801031506.m03F6DV2020709-at-ns.microscopy.com} 20, 25 -- References: {200801031506.m03F6DV2020709-at-ns.microscopy.com} 20, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 20, 25 -- Content-type: text/plain; charset=US-ASCII 20, 25 -- Content-transfer-encoding: 7BIT 20, 25 -- Content-description: Mail message body 20, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Ok. Before you say that I'm glutton for punishment, just remember that I really love playing with these kinds of toys...
I just picked up an ISI Mini-SEM, and got it home to play with. It looked simple enough that I could start breaking apart the systems of the SEM and analyzing them independently. I've got the main blue control box with two modules in it, a brown box with what I assume is the HV system, the chamber and column assembly (This is the smallest chamber I've ever seen!), and the tan voltage converter box. Is there one more piece somewhere that I should have? Does anybody have any documentation on one of these that they could copy a page or two for me?
Thanks,
Justin A. Kraft
==============================Original Headers============================== 4, 27 -- From kraftpiano-at-gmail.com Fri Jan 4 14:49:58 2008 4, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.191]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04KnvXY018803 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 4 Jan 2008 14:49:58 -0600 4, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so5235092rvb.30 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 04 Jan 2008 12:49:57 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=UoJrkFgZvjOmsXeWVmx7z0cgeySQ64eop4mrdlZvpbI=; 4, 27 -- b=x1Xubwjgf4r/aDTxjY0beo3jKuxwTbC7F4LOKDw+MyBu7eTCSP93MNSzoUVTmR/Mjpc2rAzsZ4FLc5N/wlkRp9IvRkOnW03VMYHAj5G/yzOuPnR6JpHeaqs5321Pbnc5tMLbBsr9mcmf7+OyhOgR/uY1N+3UbWFnoU92CazLnk4= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=PMpGz08Zn0JxS3va82Mur3CpqCUc8dMK3GyuOnVTpy8l4+bh89hPwVM3VsQYavQKv+pTLbzIfQApuJASu2RLAnVWPx/RBA2Gdy6ETzvnbpgiJYfamOOljURMw5sA6mZeZ8/QTRRwrsBqWQWJEw5pZukuv8gr8K7hl+Wntt6WaqM= 4, 27 -- Received: by 10.141.172.6 with SMTP id z6mr4982758rvo.136.1199479797076; 4, 27 -- Fri, 04 Jan 2008 12:49:57 -0800 (PST) 4, 27 -- Received: by 10.140.161.11 with HTTP; Fri, 4 Jan 2008 12:49:57 -0800 (PST) 4, 27 -- Message-ID: {25e2b0d20801041249o6e2ab2fft231e300d7cb48422-at-mail.gmail.com} 4, 27 -- Date: Fri, 4 Jan 2008 15:49:57 -0500 4, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: ISI Mini-SEM 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Unlike Pat, I'm still using 5302. And unlike edelmare (sorry, seen your postings but we've never met and I don't know your name) I have always used it for EM. Did try to use it as fine grain film for pictures of gels many years ago, but when a 1 min exposure at F1.8 did not give sufficient exposure I gave that up. First, you are right, the base is more thick than the estar bases you are familiar with. I assume you have the sprocket film - Kodak stopped producing the sproketless some years ago. The problem with the sprocketless film was that apparently during the cutting process the film was friction fed. As a result you got an occasional scratch due to a piece of dust getting in the system between the gel and a roller. Scratches tended to be quite long, and gave the idea of 35mm film a bad name. There are essentially no problems with scratches on the sprocket film. The thicker base makes it also work better when loading and putting into developer spools - there is less chance of breaking the film at the sprocket hole. This means the Paterson steel spools work well.
As Pat said, the green/yellow OA filter is suffcient. Leaves plenty of light to work with in the dark room.
As far as developer - we have alwoays used the D-19 developer, full strength. Get packages to make 1G US and put into a collapsable bag - say the type juice concentrates come in for wine making kits. This reduces air head room and prevents oxidation of the developer. It will keep a lot longer that way. I'm not sure about the current status for getting developer from Kodak Canada. The last order we placed took some time. I have the recipe for making it from scratch and can send it to you next week if you need. On break for the rest of the week and not back in the lab until Monday.
I under-expose slightly and push develop. The time is 3:30 at 20-22C. If compulsive you wash with a week acetic acid stop solution (1% of stock, or about 0.4% true concentration acetic acid). Then into any clearing bath for 5 minutes.
The film has really fine grain. I regularly enlarge 10x for working prints and make publication prints at 4-5 times enlargement. You will be quite happy with the results once you get the exposure times worked out.
Since you are doing film, what chemistry are you using for your printing?
Also, Tina - if you see this - sorry about the Sugar Bowl, but if I'b been in New Orleans, the Rainbow Warriors would have been worth the price of admission alone.
And regards the World Under 20's in Pardubice - for the rest of you guys on the wrong side of the 49th - final score Canada 4, US 1
Go Canada Go.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Cell:204-781-6982 Fax:204-789- 3926
==============================Original Headers============================== 13, 22 -- From paul_hazelton-at-umanitoba.ca Fri Jan 4 15:32:53 2008 13, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04LWr6O032295 13, 22 -- for {microscopy-at-microscopy.com} ; Fri, 4 Jan 2008 15:32:53 -0600 13, 22 -- Received: from [192.168.100.101] (wnpgmb01dc2-104-85.dynamic.mts.net [142.161.104.85]) 13, 22 -- (authenticated bits=0) 13, 22 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m04LWm3b015751 13, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 13, 22 -- Fri, 4 Jan 2008 15:32:50 -0600 (CST) 13, 22 -- Message-ID: {477EA600.90903-at-umanitoba.ca} 13, 22 -- Date: Fri, 04 Jan 2008 15:32:48 -0600 13, 22 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 13, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 13, 22 -- X-Accept-Language: en-us, en 13, 22 -- MIME-Version: 1.0 13, 22 -- To: susan.trant-at-viha.ca, Microscopy Listserver {microscopy-at-microscopy.com} , 13, 22 -- Philip Oshel {oshel1pe-at-cmich.edu} 13, 22 -- Subject: Re: [Microscopy] AskAMicroscopist: EM film 5302 13, 22 -- References: {200801031507.m03F7i5T026197-at-ns.microscopy.com} 13, 22 -- In-Reply-To: {200801031507.m03F7i5T026197-at-ns.microscopy.com} 13, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There will be a PreMeeting Workshop covering ESEM at the ACMM-20 Meeting in Perth Western Australia in Feb. 2008.
Here is the meeting URL : http://www.microscopy.org.au/ACMM20
Follow the link to Workshops.
Nestor Your Friendly Neighborhood SysOp
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 14 -- From zaluzec-at-microscopy.com Sat Jan 5 09:48:15 2008 11, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 11, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m05FmEKJ027791; 11, 14 -- Sat, 5 Jan 2008 09:48:14 -0600 11, 14 -- Mime-Version: 1.0 11, 14 -- Message-Id: {p06240800c3a55694015d-at-[206.69.208.22]} 11, 14 -- In-Reply-To: {200801050918.m059IOem029941-at-ns.microscopy.com} 11, 14 -- References: {200801050918.m059IOem029941-at-ns.microscopy.com} 11, 14 -- Date: Sat, 5 Jan 2008 09:48:13 -0600 11, 14 -- To: microscopy-at-microscopy.com 11, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 11, 14 -- Subject: Re: Training Course on ESEM 11, 14 -- Cc: mmiralles-at-pi.ac.ae 11, 14 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
1.) I would definitely switch to LR white. We never use methacrylate resins anymore.
2.) The flat-bottomed capsules recommended by Tobias are much better for polymerizing LR white than BEEM-type capsules. The walls seem to be thicker and are therefore less permeable to oxygen. Also, they are offered in polypropylene. The PP capsules are really a pain to get the blocks out of, but they polymerize really nicely.
3.) I was told that putting LR white (and maybe methacrylate?) resins under vacuum can cause the initiator to evaporate from the resin, causing incomplete polymerization. If you need to vacuum to remove air from your needles, I would do it during fixation or during the post-fixation wash. If you want further details, just email me.
Have fun,
Andy Bowling
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Wednesday, January 02, 2008 3:50 PM To: Bowling, Andrew
Sau, Not sure exactly if this will solve your problem, but for what its worth, you can get capsules that are like BEEMs except that they have a completely flat bottom. They are made by TAAB but available from major supply houses (no finanical connection). This gives a nice 7 mm (or so) flat surface. You can also get this out of a standard BEEM by flipping it upside down, although you have to mess about to keep the resin from leaking (definitely a mess, but possible to do).
Hope this helps, Tobias
} } Hi, } } Happy New Year!! } } Has anyone embedded pine needles in JB-4 and sectioned for LM? I had } problem with orientation when I used plastic beem capsules before. I } have been trying silicon rubber molds under 10psi. But it doesn't } polymerize competely. What is the max psi i can go not to damage both } tissue and plastic. } I have been also thinking of switching to LR-white. } But I dont know if i get better result. I would appreciate any kind of } advice on this. } } Thanks. } } Sau Silwal }
Some methycrylates have significant advantages over LR White. We routinely use a mixture of butyl and methylmethacrylate (BMMA) that we got from a paper by Tobias Baskin. This resin can be removed from sections using acetone in a manner analogous to xylene treatment of paraffin sections. This greatly increases immunostaining. Unlike JB-4, this resin easily cuts on water filled troughs. Since it is made from generic methacrylate resins, it is less expensive than the proprietary formulations. It is no good for TEM but you can't have everything. For TEM immunocytochemistry, I prefer LR Gold to LR White since I feel it cuts better with similar immunoreactivity.
-----Original Message----- X-from: Andrew.Bowling-at-ARS.USDA.GOV [mailto:Andrew.Bowling-at-ARS.USDA.GOV] Sent: Saturday, January 05, 2008 1:14 PM To: Phillips, Thomas E.
Sal,
1.) I would definitely switch to LR white. We never use methacrylate resins anymore.
2.) The flat-bottomed capsules recommended by Tobias are much better for polymerizing LR white than BEEM-type capsules. The walls seem to be thicker and are therefore less permeable to oxygen. Also, they are offered in polypropylene. The PP capsules are really a pain to get the blocks out of, but they polymerize really nicely.
3.) I was told that putting LR white (and maybe methacrylate?) resins under vacuum can cause the initiator to evaporate from the resin, causing incomplete polymerization. If you need to vacuum to remove air from your needles, I would do it during fixation or during the post-fixation wash. If you want further details, just email me.
Have fun,
Andy Bowling
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Wednesday, January 02, 2008 3:50 PM To: Bowling, Andrew
Sau, Not sure exactly if this will solve your problem, but for what its worth, you can get capsules that are like BEEMs except that they have a completely flat bottom. They are made by TAAB but available from major supply houses (no finanical connection). This gives a nice 7 mm (or so) flat surface. You can also get this out of a standard BEEM by flipping it upside down, although you have to mess about to keep the resin from leaking (definitely a mess, but possible to do).
Hope this helps, Tobias
} } Hi, } } Happy New Year!! } } Has anyone embedded pine needles in JB-4 and sectioned for LM? I had } problem with orientation when I used plastic beem capsules before. I } have been trying silicon rubber molds under 10psi. But it doesn't } polymerize competely. What is the max psi i can go not to damage both } tissue and plastic. } I have been also thinking of switching to LR-white. } But I dont know if i get better result. I would appreciate any kind of } advice on this. } } Thanks. } } Sau Silwal }
Marissa, If it turns out that your chamber is glass (which I suspect), after you get it clean, buy yourself a can of unscented White Rain hairspray and spray the inside surface of the glass tube (I would hesitate to do this to plastic because of the possibility of incompatible solvents). The next time you want to clean, just place the glass in hot soapy water. When the hairspray dissolves, your Au/Pd will also depart with little or no effort, and no scratches or cut fingers.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Friday, January 04, 2008 1:02 PM To: kenconverse-at-qualityimages.biz
Plastic encasing? Are you sure? Our Denton Desk II has a glass cylinder, which we regualrly clean (the inside) of simply but carefully running a new (cleaned) razor blade around. Slides on the glass and peels of the metal. We remove the L-seals, and then we use an ethanol soaked cotton coth to remove any remaining bits and finger Prints. The aluminium bits we polish with a "Green-Scratchey-thing" (3M) and more ethanol, then wiped down with toweling.
The only "Plastic" is the teflon bits inside, and again we use ethanol and paper towels or cotton cloth to polish them up. The case work is painted metals and we use general purpose glass/surface cleaner on it.
On 3 Jan 2008 at 14:58, mlibbee-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa } } Title-Subject: [Filtered] Denton Desk II Maintenance } } Question: Greetings! I want to clean up the Denton Desk II sputter } coater (equipped with AuPd target) in my lab facility. Does anyone } have any suggestions as to how I to clean the plastic encasing? Are } there any solvents safe to use for this purpose? } } Thanks and Happy New Year!! } } Marissa } } Login Host: 63.163.107.100 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu Jan 3 13:58:09 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03Jw9hA001680 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 13:58:09 -0600 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c3a2eec41505-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 3 Jan 2008 13:58:07 -0600 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Denton Desk II Maintenance } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 4 11:57:15 2008 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04HvF9R021636 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Jan 2008 11:57:15 -0600 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04HulEV020683; 10, 25 -- Fri, 4 Jan 2008 12:56:47 -0500 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Hukvd000531; 10, 25 -- Fri, 4 Jan 2008 12:56:46 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: "mlibbee-at-gmail.com" {mlibbee-at-gmail.com} 10, 25 -- Date: Fri, 04 Jan 2008 12:56:46 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] viaWWW: Denton Desk II Maintenance 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {477E2D0E.8384.5D96491-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- References: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 26 -- From kenconverse-at-qualityimages.biz Sat Jan 5 19:28:32 2008 25, 26 -- Received: from dpmailmta05.doteasy.com (dpmailmta05-14.doteasy.com [65.61.218.94]) 25, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m061SWeY031404 25, 26 -- for {microscopy-at-msa.microscopy.com} ; Sat, 5 Jan 2008 19:28:32 -0600 25, 26 -- Received: from dpmail16.doteasy.com (unverified [192.168.101.16]) 25, 26 -- by dpmailmta05.doteasy.com (DEO) with ESMTP id 20210645-1814644 25, 26 -- for {microscopy-at-msa.microscopy.com} ; Sat, 05 Jan 2008 17:27:43 -0800 25, 26 -- Received: from cpe-72-227-100-25.maine.res.rr.com [72.227.100.25] by dpmail16.doteasy.com with SMTP; 25, 26 -- Sat, 5 Jan 2008 17:28:12 -0800 25, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 26 -- To: "MSA Listserver" {Microscopy-at-msa.microscopy.com} 25, 26 -- Subject: RE: [Microscopy] Re: viaWWW: Denton Desk II Maintenance 25, 26 -- Date: Sat, 5 Jan 2008 20:28:07 -0500 25, 26 -- Message-ID: {000c01c85003$65155540$6401a8c0-at-Ken} 25, 26 -- MIME-Version: 1.0 25, 26 -- Content-Type: text/plain; 25, 26 -- charset="us-ascii" 25, 26 -- X-Priority: 3 (Normal) 25, 26 -- X-MSMail-Priority: Normal 25, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 25, 26 -- Importance: Normal 25, 26 -- Thread-Index: AchO+9setx1O1FT6Tjaet3pJxiYZJQBBqjkg 25, 26 -- In-Reply-To: {200801041801.m04I1e4q026009-at-ns.microscopy.com} 25, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 25, 26 -- Content-Transfer-Encoding: 8bit 25, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m061SWeY031404 ==============================End of - Headers==============================
You are invited to attend our upcoming live, interactive, web-based instructional microscopy seminars.
Pre-registration is required and connection lines are limited so reserve yours now. There is no charge to participate.
See further descriptions and pre-register at: http://www.magbiosystems.com/education
Coming up this week (January 7 - 11):
========================================================== "Quantitative Image and Data Acquisition for Fluorescent Specimens" Advice from a Facility Director
Presented by Brian Matsumoto, Ph.D., University of California, Santa Barbara
I recommend Adobe Photoshop with plug-in Image Processing Tool Kit or FoveaPro, both from Reindeer Graphics for your image analysis needs.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
rao5-at-hotmail.c om To gary.m.brown-at-exxonmobil.com 12/26/07 09:14 cc AM Subject [Microscopy] viaWWW: Image Please respond processing software to rao5-at-hotmail.c om
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Question: Hi All, Greetings for Merry X-mas and New Year.... Is there any general purpose image processing software that can do grain size analysis? I like to process AFM, SEM and TEM images eithr in their native format or as bitmaps.
Also, do you recommend any software for processing Selective area diffraction patterns? currently I write IDL code again, not very easy for students to use.
In both cases the software is intended to be used for use in teaching & research both. Price is an important concern
This Question was submitted to Ask-A-Microscopist by (pmccurdy-at-lamar.colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 7, 2008 at 12:51:06 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both pmccurdy-at-lamar.colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: pmccurdy-at-lamar.colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Title: Cleaning a Moly aperture
Question: Is it possible to use an argon ion gun to clean a moly SEM aperture? If not, why not?
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Email: andrea-at-ncmir.ucsd.edu Name: Andrea Thor
Organization: UCSD
Title-Subject: [Filtered] problems cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface
Question: I am having some problems in cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface. I heard this is not uncommon when pushing the section thickness to the extreme (such as 3-5 microns). We usually deal with the situation by refacing the block after each thick section, but this of course would make true "serial" sectioning impossible. The blocks I am working with are either cardic left ventricle or striatal tissue embedded in Durcupan.
If anyone has tried or has a protocol or techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
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Email: levilr-at-hughes.net Name: Lee Levine
Organization: Consultant
Title-Subject: [Filtered] Goniometer eyepiece
Question: I saw a simple goniometer eyepiece for measuring contact angle. Does anyone know who sells these?
Hello Andrea, What kind of knife are you using? I get good results cutting serial thick sections of resin embedded tissues from 1 to 5 microns with a Diatome Diamond Histo-Knife. I have never experienced "pitting" of the block face. I have my own bag of tricks for collecting sections as they sometimes curl up like a wood shaving as they come off the knife especially the thicker ones. Dean Abel Biological Sciences University of Iowa Iowa City IA 52242
At 03:56 PM 1/7/2008, you wrote:
} Email: andrea-at-ncmir.ucsd.edu } Name: Andrea Thor } Organization: UCSD } } Title-Subject: problems cutting serial thick sections (above 1-2 } microns) without getting serious pitting of the blockface } } Question: I am having some problems in cutting serial thick sections } (above 1-2 microns) without getting serious pitting of the } blockface. I heard this is not uncommon when pushing the section } thickness to the extreme (such as 3-5 microns). We usually deal } with the situation by refacing the block after each thick section, } but this of course would make true "serial" sectioning impossible. } The blocks I am working with are either cardic left ventricle or } striatal tissue embedded in Durcupan. } } If anyone has tried or has a protocol or techniques, I'd be very } grateful to hear about them. It could save us tons of time and } frustration as we develop a new set of protocols. Thanks very much. } } Andrea
==============================Original Headers============================== 5, 22 -- From dean-abel-at-uiowa.edu Mon Jan 7 16:51:32 2008 5, 22 -- Received: from itsnt171.iowa.uiowa.edu (itsnt171.iowa.uiowa.edu [128.255.83.12]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m07MpWGB000837 5, 22 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 7 Jan 2008 16:51:32 -0600 5, 22 -- Received: from itsnt316.iowa.uiowa.edu ([128.255.62.155]) by itsnt171.iowa.uiowa.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 22 -- Mon, 7 Jan 2008 16:51:31 -0600 5, 22 -- Received: from abel01.uiowa.edu (128.255.62.21) by email.uiowa.edu 5, 22 -- (128.255.62.155) with Microsoft SMTP Server (TLS) id 8.0.744.0; Mon, 7 Jan 5, 22 -- 2008 16:51:30 -0600 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 5, 22 -- Date: Mon, 7 Jan 2008 16:51:33 -0600 5, 22 -- To: Andrea Thor {andrea-at-ncmir.ucsd.edu} , 5, 22 -- Microscopy Society of America 5, 22 -- {Microscopy-at-msa.microscopy.com} 5, 22 -- From: Dean Abel {dean-abel-at-uiowa.edu} 5, 22 -- Subject: Re: [Microscopy]: problems cutting serial thick sections 5, 22 -- In-Reply-To: {200801072156.m07LuKJ6002422-at-ns.microscopy.com} 5, 22 -- References: {200801072156.m07LuKJ6002422-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 22 -- Message-ID: {ef904fdf-3d96-45de-8040-d01d1f15cef8} 5, 22 -- X-OriginalArrivalTime: 07 Jan 2008 22:51:31.0301 (UTC) FILETIME=[D8614950:01C8517F] ==============================End of - Headers==============================
Instead of using "Technovit 3040", I am looking for an alternative glue for methyl methacrylate (Technovit 9100 Embedding Medium).
Any suggestions would be appreciated.
What I need is: - a mounting medium/glue/adhesive of some sort to attach metal stubs to specimen which have been embedded in methyl methacrylate (Technovit 9100 Embedding Medium).
The company has suggested Technovit* 3040 which: (1) is a yellow, fast-curing (5-10 minutes, depending on room temperature) methyl methacrylate-based resin.
(2) has a "chemical composition that warrants a firm, durable bond with Technovit and secure fixing of the specimens to the Histobloc."
(3) consists of "two components- powder and liquid- allowing simple mixing, easy adhering to the specimen, and fast curing. For fixing the mounts, a highly viscous consistency (i.e., a mixing ratio of approximately 2-3 parts per volume powder: 1 part per volume liquid) has proven to be the most advantageous."
Thanks in advance,
Fanny Chu Ultrastructural Imaging The James C Hogg iCAPTURE Lab, University of British Columbia, St. Paul's Hospital Site Rm 166, 1081 Burrard St, Vancouver, BC, Canada (604) 806-8346, x62712, x62703
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==============================Original Headers============================== 11, 17 -- From FChu-at-mrl.ubc.ca Mon Jan 7 17:34:00 2008 11, 17 -- Received: from mail.mrl.ubc.ca (mail.mrl.ubc.ca [137.82.67.155]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m07NXwGv014331 11, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 17:33:59 -0600 11, 17 -- Received: from mrlmail-MTA by mail.mrl.ubc.ca 11, 17 -- with Novell_GroupWise; Mon, 07 Jan 2008 15:33:33 -0800 11, 17 -- Message-Id: {4782462A.EB79.00BC.0-at-mrl.ubc.ca} 11, 17 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 11, 17 -- Date: Mon, 07 Jan 2008 15:32:59 -0800 11, 17 -- From: "Fanny Chu" {FChu-at-mrl.ubc.ca} 11, 17 -- To: {Microscopy-at-microscopy.com} 11, 17 -- Subject: Adhesive for methyl methacrylate (Technovit 9100 Embedding 11, 17 -- Medium) 11, 17 -- Mime-Version: 1.0 11, 17 -- Content-Type: text/plain; charset=ISO-8859-15 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- Content-Disposition: inline ==============================End of - Headers==============================
Hi, For what its worth, I have glued methacrylate blocks (mixtures of methyl and butyl) to epoxy stubs with cyanoacrylate type adhesives (brand name Krazy Glue, among others). THese adhesives are known in general to stick to metal, and they are cheap and available at any hardware store. Might work?
In this case, I hope you *do* get stuck! 8--).
Tobias
} } } Instead of using "Technovit 3040", I am looking for an alternative glue } for methyl methacrylate (Technovit 9100 Embedding Medium). } } Any suggestions would be appreciated. } } What I need is: } - a mounting medium/glue/adhesive of some sort to attach metal stubs to } specimen which have been embedded in methyl methacrylate (Technovit 9100 } Embedding Medium). } } The company has suggested Technovit* 3040 which: } (1) is a yellow, fast-curing (5-10 minutes, depending on room } temperature) methyl methacrylate-based resin. } } (2) has a "chemical composition that warrants a firm, durable bond with } Technovit and secure fixing of the specimens to the Histobloc." } } (3) consists of "two components- powder and liquid- allowing simple } mixing, easy adhering to the specimen, and fast curing. For fixing the } mounts, a highly viscous consistency (i.e., a mixing ratio of } approximately 2-3 parts per volume powder: 1 part per volume liquid) has } proven to be the most advantageous." } } Thanks in advance, } } Fanny Chu } Ultrastructural Imaging } The James C Hogg iCAPTURE Lab, } University of British Columbia, } St. Paul's Hospital Site } Rm 166, 1081 Burrard St, } Vancouver, BC, Canada } (604) 806-8346, x62712, x62703 }
My bean counters are at it again. I have been asked to look into the ways other labs charge for using equipment.
We have always charged by the hour as recorded on a meter that runs when the filament is on. Minimum charge is 0.1 hour. No charges for using specimen prep equipment or for my time other than the cost of consumable supplies.
They are asking about things like minimum charges, like 1 hour minimum to start, charging based on time and extent of 'room' use, charging a portion of my salary in addition to filament time, etc.
It is pretty much a brainstorming activity for now, so anything goes.
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 10, 20 -- From jmkrupp-at-ucsc.edu Mon Jan 7 18:10:22 2008 10, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m080AMO1007324 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 18:10:22 -0600 10, 20 -- Received: from [128.114.125.120] (HELO ucsc.edu) 10, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 20 -- with ESMTPS id 28141835 for microscopy-at-microscopy.com; Mon, 07 Jan 2008 16:10:17 -0800 10, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 10, 20 -- with PIPE id 39555430; Mon, 07 Jan 2008 16:10:16 -0800 10, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 10, 20 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 10, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 10, 20 -- with ESMTPA id 39555356 for microscopy-at-microscopy.com; Mon, 07 Jan 2008 16:10:00 -0800 10, 20 -- Mime-Version: 1.0 10, 20 -- Message-Id: {p06230905c3a86e0bb434-at-[128.114.25.127]} 10, 20 -- Date: Mon, 7 Jan 2008 16:09:58 -0800 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 20 -- Subject: How do you charge for use? 10, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Overview: Schafer Corporation, Sunol, CA, is seeking a skilled and innovative Staff Engineer / Scientist to add to our mass spectrometry group. SVL performs materials characterization and related analytical services on commercial and government contracts. The activities of the group include chemical and elemental analysis of materials on a production basis, maintenance of several mass spectrometers and ancillary equipment, development of new and improved analysis techniques, interpretation, and quality assurance of analytical data. The successful candidate will primarily write software, operate instruments, perform QC/QA, reduce, and analyze data.
Responsibilities: Primary duties include scientific analytical programming, instrument operation and maintenance, developing and optimizing analytical techniques, statistical analysis, and data interpretation. Will prepare and make presentations on technical findings. As a lead engineer or scientist, this individual will be a key resource for Schafer and our customers, maintaining our expertise in the field of high sensitivity, high precision isotopic analysis.
Qualifications: The ideal candidate must have a strong background in materials science or engineering, or a related field of physics, geology, chemistry, statistics, or mathematics. Should have experience in a scientific laboratory, preferably operating SIMS, TIMS, SEM, or TEM. Experience with high vacuum technology, electronics, mechanical design, cryogenic system, and ion optics is helpful. Should have demonstrated scientific programming experience (such as Labview or FORTRAN), including statistical analysis and data interpretation. Database familiarity is helpful. Must be able to work independently and as part of a team of scientists, engineers, and technicians. Good customer service focus and commitment to quality are required.
Other qualifications include: • Bachelor's degree in physical science or engineering with minimum 5 years of technical experience. • Demonstrated ability to perform complex professional engineering / scientific work, including scientific analysis, planning, and execution. • Analytical laboratory experience in software, statistics, data evaluation, and quality control. • Proven ability to clearly write and present scientific reports and proposals. • Must be a US citizen with the ability to obtain government security clearance.
Apply at: http://jobs-schafer.icims.com/schafer_jobs/jobs/candidate/job.jsp?jobid=1331 &mode=view Schafer Corporation is an Affirmative Action, Equal Opportunity Employer.
David Sams, Ph.D. Group Leader, Mass Spectrometry AEM Group Schafer Laboratories 6705 Vallecitos Rd. Sunol, CA 94586 dsams-at-schaferlabs.com
==============================Original Headers============================== 8, 20 -- From dsams-at-schaferlabs.com Mon Jan 7 18:37:27 2008 8, 20 -- Received: from mail.schaferlabs.com (mx1.schaferlabs.com [64.168.91.154]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m080bQBS020270 8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 18:37:26 -0600 8, 20 -- Received: from dsamsws ([10.10.10.1]) 8, 20 -- by mail.schaferlabs.com (8.12.11.20060308/8.12.11) with ESMTP id m080aKdq028197 8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 16:36:20 -0800 8, 20 -- Message-Id: {200801080036.m080aKdq028197-at-mail.schaferlabs.com} 8, 20 -- From: "David Sams" {dsams-at-schaferlabs.com} 8, 20 -- To: {Microscopy-at-microscopy.com} 8, 20 -- Subject: Job Posting: Mass Spectrometry Staff Engineer / Scientist 8, 20 -- Date: Mon, 7 Jan 2008 16:37:27 -0800 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 8, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 8, 20 -- Thread-Index: AchRjqTg+nCEQmc9SX2ktcftfgYhrg== 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m080bQBS020270 ==============================End of - Headers==============================
We used to do this, indeed, we were supposed to operate under "full cost recovery". However, in their wisdom(?), the corporate types in our organisation decided that any "unit" with operational costs (including salaries) less than $1 million per year could stop charging like this and be funded entirely from overheads. We still have to charge outsiders, and still record instrument use, but no more charging. From one extreme to the other.....
cheers, rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
On 8/1/08 11:15 AM, "jmkrupp-at-ucsc.edu" {jmkrupp-at-ucsc.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } My bean counters are at it again. I have been asked to look into the } ways other labs charge for using equipment. } } We have always charged by the hour as recorded on a meter that runs } when the filament is on. Minimum charge is 0.1 hour. No charges for } using specimen prep equipment or for my time other than the cost of } consumable supplies. } } They are asking about things like minimum charges, like 1 hour } minimum to start, charging based on time and extent of 'room' use, } charging a portion of my salary in addition to filament time, etc. } } It is pretty much a brainstorming activity for now, so anything goes. } } Jon }
==============================Original Headers============================== 9, 25 -- From prvs=Rosemary.White=886c556c5-at-csiro.au Mon Jan 7 20:50:42 2008 9, 25 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m082oeEm003539 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 20:50:41 -0600 9, 25 -- X-IronPort-AV: E=Sophos;i="4.24,255,1196600400"; 9, 25 -- d="scan'208";a="183307521" 9, 25 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 9, 25 -- by act-ironport-int.csiro.au with ESMTP; 08 Jan 2008 13:50:39 +1100 9, 25 -- Received: from EXACTN1-CBR.nexus.csiro.au ([152.83.131.131]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 9, 25 -- Tue, 8 Jan 2008 13:50:38 +1100 9, 25 -- Received: OWA.CSIRO.AU 152.83.131.131 from 138.194.3.58 138.194.3.58 via HTTP with MS-WebStorage 6.0.6249 9, 25 -- Received: 138.194.3.58 138.194.3.58 from via HTTP with MS-WebStorage 6.0.6249 9, 25 -- User-Agent: Microsoft-Entourage/11.0.0.040405 9, 25 -- Date: Tue, 08 Jan 2008 13:54:23 +1100 9, 25 -- Subject: Re: [Microscopy] How do you charge for use? 9, 25 -- From: Rosemary White {Rosemary.White-at-csiro.au} 9, 25 -- To: {jmkrupp-at-ucsc.edu} 9, 25 -- CC: {microscopy-at-microscopy.com} 9, 25 -- Message-ID: {C3A9310F.836B%Rosemary.White-at-csiro.au} 9, 25 -- In-Reply-To: {200801080015.m080F9aY017467-at-ns.microscopy.com} 9, 25 -- Mime-version: 1.0 9, 25 -- Content-type: text/plain; 9, 25 -- charset="US-ASCII" 9, 25 -- Content-transfer-encoding: 7bit 9, 25 -- X-OriginalArrivalTime: 08 Jan 2008 02:50:38.0856 (UTC) FILETIME=[40307080:01C851A1] ==============================End of - Headers==============================
I have no experience with the Coolwell cooler that you mention but the symptoms sound similar to our UK Flowcool cooler.
After 2 or 3 years the temperature readout becomes erratic and when I contacted the supplier/manufacturer they told me to replace the temperature probe in the water tank. They have supplied me with spares and on my machine it is a relatively simple operation.
If you can still contact the manufacturer maybe they can help.
Good luck.
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Jill.Verlander-at-medicine.ufl.edu
Hi Andrea,
Briefly, this type of problem is down to brittle resin blocks being sectioned at too fast a cutting speed and the thicker the section, the more likely it is to occur.
Try adjusting the hardener/plasticiser proportion of your embedding mix and/or reducing the polymerisation time/temperature to end up with a softer block.
With the blocks already processed, use the best available knife and reduce the cutting speed. If the chipping of the face recurs, your only remaining option is to reduce the section thickness.
Like Dean Abel suggests, I would be using a histodiamond (wet) with a cutting window speed of 1mm/sec and increasing the section thickness gradually from an initial 2 microns. I would also trim off most of the surrounding resin and shape the block to a point to reduce the cutting force on the knife, particularly as you are wanting to section at up to 5 microns.
Happy New Year!
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, Scotland, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: andrea-at-ncmir.ucsd.edu [mailto:andrea-at-ncmir.ucsd.edu] Sent: 07 January 2008 22:02 To: Mckinnon, Alastair D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea-at-ncmir.ucsd.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: andrea-at-ncmir.ucsd.edu Name: Andrea Thor
Organization: UCSD
Title-Subject: [Filtered] problems cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface
Question: I am having some problems in cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface. I heard this is not uncommon when pushing the section thickness to the extreme (such as 3-5 microns). We usually deal with the situation by refacing the block after each thick section, but this of course would make true "serial" sectioning impossible. The blocks I am working with are either cardic left ventricle or striatal tissue embedded in Durcupan.
If anyone has tried or has a protocol or techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
Hi Jon, We charge per/hour for equipment use: TEM, SEM, CPD, Sputter Coater, Digital LM and any dark room use (yes there are still a couple of researches using film!!).We do not allow users to process their samples here as there is not enough room nor do we train them. We can process the tissue for them at a price per every 2 samples (we figure an experimental and a control is pretty standard). This processing charge includes supplies and our time. We then charge per block for thick sections and another charge for thin sectioning. Our time is charged at rates that take into consideration who's hands are helping/training: SRA I, SRAII or SRAIV. We do have a few supplies we will sell to the researcher at our cost (tax and shipping figured into the equation): stubs, coated grids, 16% paraformaldehyde, etc. Hope this helps! Pat Kysar University of California, Davis Medical School, Pathology EM Lab 530-752-4701
----- Original Message ----- X-from: {jmkrupp-at-ucsc.edu} To: {pekysar-at-ucdavis.edu} Sent: Monday, January 07, 2008 4:15 PM
Dear Microscopists,
The first update of the list of meetings for microscopists in the year 2008 has been finished at the Petr's Microscopy Resources at the
Your submission of other meetings will be very appreciated (submission form is accessible from the page mentioned, the direct link for the submission is http://www.petr.isibrno.cz/microscopy/PMRform.php).
Regards, Petr Schauer ******************************************** Dr. Petr Schauer Scintillation and Cathodoluminescent Systems Institute of Scientific Instruments, AS CR, v.v.i. Academy of Sciences of the Czech Republic Kralovopolska 147, CZ-61264 Brno, Czech Republic ******************************************** tel.: +420 541 514 313 fax : +420 541 514 404, or +420 541 514 402 e-mail: petr-at-isibrno.cz www: http://www.petr.isibrno.cz/ ********************************************
==============================Original Headers============================== 6, 18 -- From PetrS-at-ISIBrno.Cz Wed Jan 9 08:39:46 2008 6, 18 -- Received: from pecka.isibrno.cz (pecka.isibrno.cz [195.178.70.10]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09EdhBT031987 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Jan 2008 08:39:45 -0600 6, 18 -- Received: from [195.178.70.170] (aligator.isibrno.cz [195.178.70.170]) 6, 18 -- by pecka.isibrno.cz (8.14.1/8.14.1) with ESMTP id m09EdPk3022178 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Jan 2008 15:39:25 +0100 (CET) 6, 18 -- (envelope-from PetrS-at-ISIBrno.Cz) 6, 18 -- Message-ID: {4784DC99.3060909-at-ISIBrno.Cz} 6, 18 -- Date: Wed, 09 Jan 2008 15:39:21 +0100 6, 18 -- From: Petr Schauer {PetrS-at-ISIBrno.Cz} 6, 18 -- Organization: ISI ASCR Brno 6, 18 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy ListServer {Microscopy-at-Microscopy.Com} 6, 18 -- Subject: Meetings for Microscopists in 2008 6, 18 -- Content-Type: text/plain; charset=ISO-8859-2; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am looking for a free software to convert eds to emsa extension file.
I am using an old software for Thermo/Noran EDS. It has two types of file format: Vantage (eds extension) and EMSA (emsa extension). Recent NORAN System Six can read emsa format, but not eds format.
I can open eds file using old software and save as emsa format. However, it is inconvenient. Is there any alternative method (like free software) to convert eds format to emsa format? Please advise.
Thank you,
Hiromi Konishi, Ph.D. UW-MADISON
==============================Original Headers============================== 6, 29 -- From hikonishi3-at-gmail.com Wed Jan 9 09:24:37 2008 6, 29 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.232]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09FOX31007588 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jan 2008 09:24:36 -0600 6, 29 -- Received: by wx-out-0506.google.com with SMTP id h30so120951wxd.21 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 09 Jan 2008 07:24:31 -0800 (PST) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 6, 29 -- bh=FxqSWvI1r65GAqgIcGjCQRehRahdZYVJZHkeOqMso6o=; 6, 29 -- b=XjUM7RL0u5pnygLItvC9PnsZgPRpRn4n6pd1J6WMM0CC0I1rKbGptr+wo+rAV1o2RytREUSLEdG/tcQbDWa7IWjlzjtlAcWJkvZ0eXYV6s//BZsfoQrweQEz3IgVUHlFtCxbdhT+CKpZW/G/9Y6iq4TW1k7HMvINljPRO/9jV+E= 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 6, 29 -- b=oiMxOou6cmZL1AQAJxFDY4B6plneFMaPknYID6zK2c1jPW1ttAah9C8QMOj6XFeu6NyZgRY8gkPaTFsYo3QSsCwUlj8PrIqNvPNbBNVcUyDMEBVGdWPozyzzgEGAu78bD/8QAeRqosHlNPtES7cGrMheDrqlCUQpUuXqLr2/ZkM= 6, 29 -- Received: by 10.142.102.5 with SMTP id z5mr314726wfb.15.1199892270489; 6, 29 -- Wed, 09 Jan 2008 07:24:30 -0800 (PST) 6, 29 -- Received: by 10.143.6.4 with HTTP; Wed, 9 Jan 2008 07:24:30 -0800 (PST) 6, 29 -- Message-ID: {456d33d00801090724k6f86a0du6643470c3b98a2a6-at-mail.gmail.com} 6, 29 -- Date: Wed, 9 Jan 2008 09:24:30 -0600 6, 29 -- From: "Hiromi Konishi" {hkonishi-at-wisc.edu} 6, 29 -- Sender: hikonishi3-at-gmail.com 6, 29 -- To: Microscopy-at-microscopy.com 6, 29 -- Subject: Thermo/Noran EDS: How to convert eds to emsa file? 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- Content-Disposition: inline 6, 29 -- X-Google-Sender-Auth: e7e12a6676b9b5ff ==============================End of - Headers==============================
Hiromi, Speak with your Thermo service folks. There is a software application they have that converts .eds format (and all the other Vantage file formats .grey etc.) to formats that are compatible with the System SIX software. It is intended as a one-time only tool to be used during the upgrade from a Vantage system to a System SIX system, but if you speak with their technical service folks, they might be able to work out a solution for you.
Good Luck, Bryan Bandli
hkonishi-at-wisc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello: } } I am looking for a free software to convert eds to emsa extension file. } } I am using an old software for Thermo/Noran EDS. It has two types of } file format: Vantage (eds extension) and EMSA (emsa extension). Recent } NORAN System Six can read emsa format, but not eds format. } } I can open eds file using old software and save as emsa format. } However, it is inconvenient. Is there any alternative method (like } free software) to convert eds format to emsa format? Please advise. } } Thank you, } } Hiromi Konishi, Ph.D. } UW-MADISON } } ==============================Original Headers============================== } 6, 29 -- From hikonishi3-at-gmail.com Wed Jan 9 09:24:37 2008 } 6, 29 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.232]) } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09FOX31007588 } 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jan 2008 09:24:36 -0600 } 6, 29 -- Received: by wx-out-0506.google.com with SMTP id h30so120951wxd.21 } 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 09 Jan 2008 07:24:31 -0800 (PST) } 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 29 -- d=gmail.com; s=gamma; } 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; } 6, 29 -- bh=FxqSWvI1r65GAqgIcGjCQRehRahdZYVJZHkeOqMso6o=; } 6, 29 -- b=XjUM7RL0u5pnygLItvC9PnsZgPRpRn4n6pd1J6WMM0CC0I1rKbGptr+wo+rAV1o2RytREUSLEdG/tcQbDWa7IWjlzjtlAcWJkvZ0eXYV6s//BZsfoQrweQEz3IgVUHlFtCxbdhT+CKpZW/G/9Y6iq4TW1k7HMvINljPRO/9jV+E= } 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 6, 29 -- d=gmail.com; s=gamma; } 6, 29 -- h=message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; } 6, 29 -- b=oiMxOou6cmZL1AQAJxFDY4B6plneFMaPknYID6zK2c1jPW1ttAah9C8QMOj6XFeu6NyZgRY8gkPaTFsYo3QSsCwUlj8PrIqNvPNbBNVcUyDMEBVGdWPozyzzgEGAu78bD/8QAeRqosHlNPtES7cGrMheDrqlCUQpUuXqLr2/ZkM= } 6, 29 -- Received: by 10.142.102.5 with SMTP id z5mr314726wfb.15.1199892270489; } 6, 29 -- Wed, 09 Jan 2008 07:24:30 -0800 (PST) } 6, 29 -- Received: by 10.143.6.4 with HTTP; Wed, 9 Jan 2008 07:24:30 -0800 (PST) } 6, 29 -- Message-ID: {456d33d00801090724k6f86a0du6643470c3b98a2a6-at-mail.gmail.com} } 6, 29 -- Date: Wed, 9 Jan 2008 09:24:30 -0600 } 6, 29 -- From: "Hiromi Konishi" {hkonishi-at-wisc.edu} } 6, 29 -- Sender: hikonishi3-at-gmail.com } 6, 29 -- To: Microscopy-at-microscopy.com } 6, 29 -- Subject: Thermo/Noran EDS: How to convert eds to emsa file? } 6, 29 -- MIME-Version: 1.0 } 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 } 6, 29 -- Content-Transfer-Encoding: 7bit } 6, 29 -- Content-Disposition: inline } 6, 29 -- X-Google-Sender-Auth: e7e12a6676b9b5ff } ==============================End of - Headers============================== } }
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==============================Original Headers============================== 6, 20 -- From bbandli-at-mvainc.com Wed Jan 9 09:59:25 2008 6, 20 -- Received: from smtp03.atlngahp.sys.nuvox.net (smtp-out3.atlngahp.sys.nuvox.net [70.43.63.20]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09FxMxs027503 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jan 2008 09:59:24 -0600 6, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 6, 20 -- by smtp03.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id m09FxFO9003591; 6, 20 -- Wed, 9 Jan 2008 10:59:15 -0500 6, 20 -- Message-ID: {4784EFD7.9050000-at-mvainc.com} 6, 20 -- Date: Wed, 09 Jan 2008 11:01:27 -0500 6, 20 -- From: bbandli {bbandli-at-mvainc.com} 6, 20 -- Reply-To: bbandli-at-mvainc.com 6, 20 -- Organization: MVA Scientific Consultants 6, 20 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: hkonishi-at-wisc.edu, Microscopy-at-microscopy.com 6, 20 -- Subject: Re: [Microscopy] Thermo/Noran EDS: How to convert eds to emsa file? 6, 20 -- References: {200801091526.m09FQ5pG008831-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200801091526.m09FQ5pG008831-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Is there some one who knows how to calculate number fractions (not length or pixellength fractions) of CSL boundaries (or grainboundaries in general) from TSL and / or HKL Channel Five datasets?
Thanks for thinking along with me,
Corrie ----------------------------------------------------------- Corrie van Hoek
CORUS RD & T Ceramics Research Center Building Code 3J-22 PO Box 1000 1970 CA Ijmuiden The Netherlands tel. 02514 92626 fax. 02514 70489
********************************************************************** This transmission is confidential and must not be used or disclosed by anyone other than the intended recipient. Neither Corus Group Limited nor any of its subsidiaries can accept any responsibility for any use or misuse of the transmission by anyone.
For address and company registration details of certain entities within the Corus group of companies, please visit http://www.corusgroup.com/entities
Hi! During some works in a room next to our TEM, one worker opened a canalisation of the watercooling system, emptying the water tank of the watercooling device. I filled it again with normal tap water but I think I have to add some product to avoid mold growth. Could you recommend one such product and where I can order it, especially those of you from Germany and Austria (we are located in Vienna, Austria)? Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM 1000S.
Thank you in advance, Stephane
____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 4, 19 -- From nizets2-at-yahoo.com Thu Jan 10 06:40:52 2008 4, 19 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0ACem4n001942 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 06:40:50 -0600 4, 19 -- Received: (qmail 40247 invoked by uid 60001); 10 Jan 2008 12:40:37 -0000 4, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 19 -- s=s1024; d=yahoo.com; 4, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 4, 19 -- b=Fn3gG5utMTGOd4DpVnbGJeUkMJ1vkheZBOP1Zlai3H7cinr/ZsdrN2y8+5E+BNqTAFvpRmsa+7R7cpvr9Lm5XjgKsb5AtFa1hKi8ajiPOcAevuTeV8DGXVwJ2vzxC7zQMd0X1Vr8LddylbtVm86IZpCLAk6Qsq3vB0sabN3cgcs=; 4, 19 -- X-YMail-OSG: QJ3hsoAVM1l_O63TUbf1U2TeLu.w7OQGeGK7boHrsuxxVDDcpK431mVgc_WIKpM7Fmag2Pq11gv0JaKuQnqAsHtsSe3IQ5PJX6ZT2Jr5GhK0JYN7Cdg- 4, 19 -- Received: from [80.122.101.100] by web37403.mail.mud.yahoo.com via HTTP; Thu, 10 Jan 2008 04:40:37 PST 4, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.158.1 4, 19 -- Date: Thu, 10 Jan 2008 04:40:37 -0800 (PST) 4, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 4, 19 -- Subject: Product for watercooling 4, 19 -- To: microscopy-at-microscopy.com 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; charset=us-ascii 4, 19 -- Message-ID: {403898.39040.qm-at-web37403.mail.mud.yahoo.com} ==============================End of - Headers==============================
We have just inherited a BAL-TEC 060 freeze fracture system.
I would like to ask if anyone out in there Microscopy Land has similar equipment and has written their own set of operation notes for this system that they would be willing to share with us, perhaps as a pdf file or similar?
We have also inherited with the freeze fracture system a Bal-Tec SBU 020 sandblasting Unit. Does anyone have one of these also and be wiling to share the operating notes?
Many thanks, kind regards
Allan
Allan Mitchell Technical Manager Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
==============================Original Headers============================== 12, 21 -- From allan.mitchell-at-stonebow.otago.ac.nz Thu Jan 10 16:44:05 2008 12, 21 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0AMi3hZ003133 12, 21 -- for {microscopy-at-msa.microscopy.com} ; Thu, 10 Jan 2008 16:44:04 -0600 12, 21 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 12, 21 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id m0AMi1eQ013135 12, 21 -- for {microscopy-at-msa.microscopy.com} ; Fri, 11 Jan 2008 11:44:01 +1300 12, 21 -- Received: from allan.otago.ac.nz ([139.80.40.92]) 12, 21 -- by galadriel.otago.ac.nz with esmtp (Exim 4.63) 12, 21 -- (envelope-from {allan.mitchell-at-stonebow.otago.ac.nz} ) 12, 21 -- id 1JD67z-0006uh-Dn 12, 21 -- for microscopy-at-msa.microscopy.com; Fri, 11 Jan 2008 11:43:59 +1300 12, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 21 -- Content-Transfer-Encoding: 7bit 12, 21 -- Message-Id: {11C3F12C-3D84-438C-A3D5-637CB20DBFEC-at-stonebow.otago.ac.nz} 12, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 21 -- To: microscopy-at-msa.microscopy.com 12, 21 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 12, 21 -- Subject: Bal-Tec 060 Freeze Etching System 12, 21 -- Date: Fri, 11 Jan 2008 11:44:36 +1300 12, 21 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I would suggest draining the water and replacing with distilled water. The Cl in tap water could lead to corrosion of metal in contact with the water. A good additive IMO is Ethylene Glycol as a 10% mix. I use technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled water for 5 gallons total in the Haskris R050 chiller reservoir.
Sometimes this holds up for a year.
gary g.
At 04:52 AM 1/10/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0AMutZj015411 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 16:56:56 -0600 11, 20 -- Message-Id: {200801102256.m0AMutZj015411-at-ns.microscopy.com} 11, 20 -- Received: (qmail 7535 invoked from network); 10 Jan 2008 14:56:57 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 7531, pid: 7533, t: 0.0916s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp4 with SMTP; 10 Jan 2008 14:56:57 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Thu, 10 Jan 2008 14:56:53 -0800 11, 20 -- To: nizets2-at-yahoo.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Product for watercooling 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200801101252.m0ACqLYm004944-at-ns.microscopy.com} 11, 20 -- References: {200801101252.m0ACqLYm004944-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-360B2309 ==============================End of - Headers==============================
We incorporate Sigma water bath treatment in the reservoir - 3.5ml per tank full (25L).
Seems to work well - lasting about 12 months and got this recommendation from Sigma Technical Services re using it in chiller circulators for EMs.
TechServ_Number: S5525 TechServ_Name: SigmaClean; water bath treatment TechServ_Brand: SIGMA
TechServ_re: is this product recommended for use in chiller circulators for the supply of cooling water for a transmission EM (Philips CM10).
Best regards,
Alastair
Alastair McKinnon Histology & EM Facility Manager, IMS R2.62 University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: 10 January 2008 23:00 To: Mckinnon, Alastair D.
I would suggest draining the water and replacing with distilled water. The Cl in tap water could lead to corrosion of metal in contact with the water. A good additive IMO is Ethylene Glycol as a 10% mix. I use technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled water for 5 gallons total in the Haskris R050 chiller reservoir.
Sometimes this holds up for a year.
gary g.
At 04:52 AM 1/10/2008, you wrote:
} ----------------------------------------------------------------------- } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
} watercooling device. } I filled it again with normal tap water but I think I have to add some } product to avoid mold growth. } Could you recommend one such product and where I can order it, } especially those of you from Germany and Austria (we are located in } Vienna, Austria)? } Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM 1000S. } } Thank you in advance, } Stephane
==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0AMutZj015411 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 16:56:56 -0600 11, 20 -- Message-Id: {200801102256.m0AMutZj015411-at-ns.microscopy.com} 11, 20 -- Received: (qmail 7535 invoked from network); 10 Jan 2008 14:56:57 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 7531, pid: 7533, t: 0.0916s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp4 with SMTP; 10 Jan 2008 14:56:57 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 --
All, After much fighting of corrosion and algae issues I settled years ago on what might be the easiest and most long term "solution". That is pure distilled water. And here's the secret, you don't need to add any chemical additives if you remove all clear plastic hoses from the system and replace them with black or otherwise opaque materials. No light, no growth.
With this "solution" I have found I can run for years with a crystal clear tank and with only an occasional speck of rust visible in the bottom of the tank, which is probably left over from the prior years of running god know what through the lines. This "solution" is currently running in several SEMs and microprobes, using a variety of chillers (mostly Haskris which I have found to be much more reliable than Coolwell).
Works for me anyway. john
At 07:58 AM 1/11/2008, a.d.mckinnon-at-abdn.ac.uk wrote:
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==============================Original Headers============================== 8, 21 -- From donovan-at-uoregon.edu Fri Jan 11 10:37:25 2008 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGbOfe005078 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:37:24 -0600 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 8, 21 -- (authenticated bits=0) 8, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id m0BGbNgM019167 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 08:37:23 -0800 8, 21 -- Message-Id: {200801111637.m0BGbNgM019167-at-smtp.uoregon.edu} 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Fri, 11 Jan 2008 08:37:13 -0800 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} 8, 21 -- Subject: Re: [Microscopy] Product for watercooling 8, 21 -- In-Reply-To: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} 8, 21 -- References: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 21 -- X-Virus-Scanned: ClamAV 0.92/5478/Fri Jan 11 07:39:22 2008 on mserv1 8, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I second John's "solution". It's what we have been using for our FESEM for several years without any problems. Opaque lines from the chiller to the scope are essential however. Even though this is what this scope has been on since day 1, we do have a small amount of rust/grit in the bottom of the tank but it hasn't been a problem. We also change the H2O every 6months (Per JEOL service). Cheers, Bryan Bandli
donovan-at-uoregon.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } All, } After much fighting of corrosion and algae issues I settled years ago } on what might be the easiest and most long term "solution". That is } pure distilled water. And here's the secret, you don't need to add } any chemical additives if you remove all clear plastic hoses from the } system and replace them with black or otherwise opaque materials. No } light, no growth. } } With this "solution" I have found I can run for years with a crystal } clear tank and with only an occasional speck of rust visible in the } bottom of the tank, which is probably left over from the prior years } of running god know what through the lines. This "solution" is } currently running in several SEMs and microprobes, using a variety of } chillers (mostly Haskris which I have found to be much more reliable } than Coolwell). } } Works for me anyway. } john } } At 07:58 AM 1/11/2008, a.d.mckinnon-at-abdn.ac.uk wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi, } } } } We incorporate Sigma water bath treatment in the reservoir - 3.5ml per } } tank full (25L). } } } } Seems to work well - lasting about 12 months and got this recommendation } } } } from Sigma Technical Services re using it in chiller circulators for } } } EMs. } } } } TechServ_Number: S5525 } } TechServ_Name: SigmaClean; water bath treatment } } TechServ_Brand: SIGMA } } } } TechServ_re: is this product recommended for use in chiller circulators } } for } } the supply of cooling water for a transmission EM (Philips CM10). } } } } Best regards, } } } } Alastair } } } } Alastair McKinnon } } Histology & EM Facility Manager, IMS R2.62 } } University of Aberdeen, Institute of Medical Science } } Foresterhill, Aberdeen, AB25 2ZD } } tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) } } fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk } } www.abdn.ac.uk/ims/h-em } } -----Original Message----- } } X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } } Sent: 10 January 2008 23:00 } } To: Mckinnon, Alastair D. } } Subject: [Microscopy] Re: Product for watercooling } } } } } } } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ } } ---- } } } } I would suggest draining the water and replacing with distilled water. } } The Cl in tap water could lead to corrosion of metal in contact with the } } water. A good additive IMO is Ethylene Glycol as a 10% mix. I use } } technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled } } water for 5 gallons total in the Haskris R050 chiller reservoir. } } } } Sometimes this holds up for a year. } } } } gary g. } } } } } } } } At 04:52 AM 1/10/2008, you wrote: } } } } } } } } } } } } } ----------------------------------------------------------------------- } } } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } of America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } Hi! } } } During some works in a room next to our TEM, one worker opened a } } } canalisation of the watercooling system, emptying the water tank of the } } } } } } watercooling device. } } } I filled it again with normal tap water but I think I have to add some } } } product to avoid mold growth. } } } Could you recommend one such product and where I can order it, } } } especially those of you from Germany and Austria (we are located in } } } Vienna, Austria)? } } } Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM } } } } } 1000S. } } } } } Thank you in advance, } } } Stephane } } } } } ==============================Original } } Headers============================== } } 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- } } Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net } } [66.60.130.145]) } } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } id m0AMutZj015411 } } 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 } } 16:56:56 -0600 } } 11, 20 -- Message-Id: {200801102256.m0AMutZj015411-at-ns.microscopy.com} } } 11, 20 -- Received: (qmail 7535 invoked from network); 10 Jan 2008 } } 14:56:57 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 7531, pid: } } 7533, t: 0.0916s } } 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 } } 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) } } 11, 20 -- by qsmtp4 with SMTP; 10 Jan 2008 14:56:57 -0800 } } 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- } } Date: Thu, 10 Jan 2008 14:56:53 -0800 11, 20 -- To: nizets2-at-yahoo.com } } 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: } } [Microscopy] Product for watercooling 11, 20 -- Cc: MSA listserver } } {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: } } {200801101252.m0ACqLYm004944-at-ns.microscopy.com} } } 11, 20 -- References: {200801101252.m0ACqLYm004944-at-ns.microscopy.com} } } 11, 20 -- Mime-Version: 1.0 } } 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; } } x-avg-checked=avg-ok-360B2309 ==============================End of - } } Headers============================== } } } } } } } } ==============================Original Headers============================== } } 25, 24 -- From a.d.mckinnon-at-abdn.ac.uk Fri Jan 11 09:47:45 2008 } } 25, 24 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk } } [139.133.7.13]) } } 25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m0BFlgKZ023642 } } 25, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 } } 09:47:44 -0600 } } 25, 24 -- Received: from ew-mail-b.uoa.abdn.ac.uk ([139.133.15.21] } } helo=VMAIL1.uoa.abdn.ac.uk) } } 25, 24 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52) } } 25, 24 -- id 1JDM6b-00024t-4r; Fri, 11 Jan 2008 15:47:37 +0000 } } 25, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 25, 24 -- Content-class: urn:content-classes:message } } 25, 24 -- MIME-Version: 1.0 } } 25, 24 -- Content-Type: text/plain; } } 25, 24 -- charset="US-ASCII" } } 25, 24 -- Subject: RE: [Microscopy] Re: Product for watercooling } } 25, 24 -- Date: Fri, 11 Jan 2008 15:45:11 -0000 } } 25, 24 -- Message-ID: } } {009B2FCFB3DD25408A4BBC240F091376BD20A5-at-VMAIL1.uoa.abdn.ac.uk} } } 25, 24 -- X-MS-Has-Attach: } } 25, 24 -- X-MS-TNEF-Correlator: } } 25, 24 -- Thread-Topic: [Microscopy] Re: Product for watercooling } } 25, 24 -- thread-index: AchT3EvpoZa+Q4/RS2y35Xsyfvhq1gAi/Eww } } 25, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} } } 25, 24 -- To: {gary-at-gaugler.com} } } 25, 24 -- Cc: {Microscopy-at-microscopy.com} } } 25, 24 -- Content-Transfer-Encoding: 8bit } } 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id m0BFlgKZ023642 } } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 8, 21 -- From donovan-at-uoregon.edu Fri Jan 11 10:37:25 2008 } 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGbOfe005078 } 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:37:24 -0600 } 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) } 8, 21 -- (authenticated bits=0) } 8, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id m0BGbNgM019167 } 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 08:37:23 -0800 } 8, 21 -- Message-Id: {200801111637.m0BGbNgM019167-at-smtp.uoregon.edu} } 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 8, 21 -- Date: Fri, 11 Jan 2008 08:37:13 -0800 } 8, 21 -- To: microscopy-at-microscopy.com } 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} } 8, 21 -- Subject: Re: [Microscopy] Product for watercooling } 8, 21 -- In-Reply-To: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} } 8, 21 -- References: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} } 8, 21 -- Mime-Version: 1.0 } 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 8, 21 -- X-Virus-Scanned: ClamAV 0.92/5478/Fri Jan 11 07:39:22 2008 on mserv1 } 8, 21 -- X-Virus-Status: Clean } ==============================End of - Headers============================== } }
-- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Bryan Bandli Senior Research Scientist MVA Scientific Consultants 3300 Breckinridge Blvd., Suite 400 (770) 662-8509 bbandli-at-mvainc.com www.mvainc.com ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 5, 20 -- From bbandli-at-mvainc.com Fri Jan 11 10:56:17 2008 5, 20 -- Received: from smtp03.atlngahp.sys.nuvox.net (smtp-out3.atlngahp.sys.nuvox.net [70.43.63.20]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGuHYF017808 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:56:17 -0600 5, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 5, 20 -- by smtp03.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id m0BGuCXC012385 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 11:56:12 -0500 5, 20 -- Message-ID: {4787A036.9030005-at-mvainc.com} 5, 20 -- Date: Fri, 11 Jan 2008 11:58:30 -0500 5, 20 -- From: bbandli {bbandli-at-mvainc.com} 5, 20 -- Reply-To: bbandli-at-mvainc.com 5, 20 -- Organization: MVA Scientific Consultants 5, 20 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 5, 20 -- MIME-Version: 1.0 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- Subject: Re: Product for watercooling 5, 20 -- References: {200801111637.m0BGbreR006045-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200801111637.m0BGbreR006045-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] TEM Recommendations
Question: I work in a multi-user bio-imaging facility, and we recently were funded to purchase a new TEM. We are interested in a TEM that is EM tomography capable with a high resolution CCD camera and is user-friendly. We plan to upgrade to include a cryo-stage in the near future, so an instrument that is cryo-ready would be beneficial.
I was wondering if anyone could provide me with recommendations for TEMs and their experience with tomography. Also, is there a good test sample that can be used to test EM tomography both with cryo and conventional preparations for the demos?
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Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] service for older Reichert Ultrqacut E ultramicrotomes
Question: We have 2 older (not ancient) working Ultracut E ultramicrotomes that we would like to have routine service on (cleaning, lubricating, etc). Leica will not service them any longer. Does anyone know of an independent person for the northeast region (Boston)? Thanks in advance.
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Email: alissa.susanne-at-gmail.com Name: Alissa H
Organization: University of Maryland
Title-Subject: [Filtered] specimen preparation
Question: We have a new Zeiss AxioImager here, and I've been given the task of not only learning how to use it, but also one of my main projects involves use of the microscope to evaluate fluorescence of some bacteria. I've transformed a plasmid with YFP on it into some streptococcus. I can find the buggers, take a reasonable Z stack, and even deconvolve the image.
What I'd like to learn is better ways to prepare my samples, right now I've just been making wet mounts with saline. I'd also like to learn how to better use Zeiss' computer program that we got with the microscope. I don't find the program intuitive, like I do for most computer programs, and am getting frustrated. If anyone can give me good suggestions on where I can read about these things, I'd really appreciate it. I feel like a total newbie, and I'm not quite sure where to go to get good information.
On Jan 11, 2008, at 3:30 PM, modla-at-dbi.udel.edu wrote:
} I work in a multi-user bio-imaging facility, and we recently were } funded to purchase a new TEM. We are interested in a TEM that is } EM tomography capable with a high resolution CCD camera and is user- } friendly. We plan to upgrade to include a cryo-stage in the near } future, so an instrument that is cryo-ready would be beneficial. } } } } I was wondering if anyone could provide me with recommendations for } TEMs and their experience with tomography. Also, is there a good } test sample that can be used to test EM tomography both with cryo } and conventional preparations for the demos? } Dear Shannon, To a great extent, the type of instrument necessary depends on the specimens for which you wish to do tomography. If, for example, you want to look at bacterial cells, then you will need a higher voltage than if you only wish to look at viruses or protein complexes. Our lab has had a very good experience with FEI instruments. The Tecnai T12, which, while not our primary tomographic instrument, has both tomographic and cryo capabilities, is very reliable and user friendly. It is an off-the-shelf, but very high-end TEM. It is limited, however, to specimens thinner than a typical bacterium. Our main instrument is a Tecnai TF30H Polara with all the bells and whistles, most of which are necessary for electron cryo-tomography, especially of whole bacterial cells. It has a field-emission source, necessary for good beam coherence for phase contrast; it operates at 300 kV, which provides the penetrating power to observe ~0.5 um specimens tilted to 70 degrees (an effective thickness of 1.5 um); its cartridge system for holding specimens provides accurate and reproducible tilting for good tracking during tilt-series data collection; it has an energy filter, which provides increased contrast and resolution by looking only at elastically scattered electrons; and, finally, it is equipped with a 4k x 4k GATAN Ultracam. It too is pretty reliable, but, being more complicated than the T12, there are more things that can go wrong. The only drawback to the Polara is its price, but I would hope that any administrator who wants to get into the electron cryo-tomography field would be willing to spend what is necessary. I would suggest using a 250 nm conventional plastic section for a room temperature test object and whatever is your favorite specimen--limited to a thickness that suits the instrument--for a cryo test object. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 23 -- From tivol-at-caltech.edu Fri Jan 11 18:56:09 2008 4, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0C0u9oY017616 4, 23 -- for {microscopy-at-msa.microscopy.com} ; Fri, 11 Jan 2008 18:56:09 -0600 4, 23 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 4, 23 -- by wood-ox-postvirus (Postfix) with ESMTP id D31AA13E03; 4, 23 -- Fri, 11 Jan 2008 16:56:07 -0800 (PST) 4, 23 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 4, 23 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 78C7213E6F; 4, 23 -- Fri, 11 Jan 2008 16:56:01 -0800 (PST) 4, 23 -- In-Reply-To: {200801112330.m0BNUpBP010814-at-ns.microscopy.com} 4, 23 -- References: {200801112330.m0BNUpBP010814-at-ns.microscopy.com} 4, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 4, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 23 -- Message-Id: {7C65E385-CA51-4A97-A54D-ABBD831BD3C9-at-caltech.edu} 4, 23 -- Cc: microscopy-at-msa.microscopy.com 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 23 -- Subject: Re: [Microscopy] viaWWW: TEM Recommendations 4, 23 -- Date: Fri, 11 Jan 2008 16:55:59 -0800 4, 23 -- To: modla-at-dbi.udel.edu 4, 23 -- X-Mailer: Apple Mail (2.753) 4, 23 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (r.kirk-at-kingston.ac.uk) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, January 13, 2008 at 07:07:47 ---------------------------------------------------------------------------
Email: r.kirk-at-kingston.ac.uk Name: Dr Ruth Kirk
Organization: Kingston University
Education: Undergraduate College
Location: Surrey, UK
Question: I am currently supervising a student project which will investigate the use of different types of preparation techniques for SEM of protists. Do you have any advice on aggregating the protists during processing? That is, can you stick them to a cover slip or similar?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both panxijiang-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: panxijiang-at-gmail.com Name: Xijiang Pan
Organization: Tsinghua University
Title-Subject: [Filtered] how to perform "bake out" procedure on the FEI CM series TEM
Question: I am wondering if there is anybody in this list farmiliar with the "bake out" procedure for the FEI CM serious TEM. The local service engineers do not have any experiences in such work. So if you know,please contact me. Thanks in advance.
Just out of curiosity, since a chiller is a relatively closed system, what about putting a dilute chlorine solution in, like that used in pools? Or perhaps a slight bleach additive? Would that damage the equipment?
--Justin A. Kraft
On Jan 11, 2008 12:05 PM, {bbandli-at-mvainc.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I second John's "solution". It's what we have been using for our FESEM } for several years without any problems. Opaque lines from the chiller } to the scope are essential however. Even though this is what this scope } has been on since day 1, we do have a small amount of rust/grit in the } bottom of the tank but it hasn't been a problem. We also change the H2O } every 6months (Per JEOL service). } Cheers, } Bryan Bandli } } donovan-at-uoregon.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } All, } } After much fighting of corrosion and algae issues I settled years ago } } on what might be the easiest and most long term "solution". That is } } pure distilled water. And here's the secret, you don't need to add } } any chemical additives if you remove all clear plastic hoses from the } } system and replace them with black or otherwise opaque materials. No } } light, no growth. } } } } With this "solution" I have found I can run for years with a crystal } } clear tank and with only an occasional speck of rust visible in the } } bottom of the tank, which is probably left over from the prior years } } of running god know what through the lines. This "solution" is } } currently running in several SEMs and microprobes, using a variety of } } chillers (mostly Haskris which I have found to be much more reliable } } than Coolwell). } } } } Works for me anyway. } } john } } } } At 07:58 AM 1/11/2008, a.d.mckinnon-at-abdn.ac.uk wrote: } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi, } } } } } } We incorporate Sigma water bath treatment in the reservoir - 3.5ml per } } } tank full (25L). } } } } } } Seems to work well - lasting about 12 months and got this recommendation } } } } } } from Sigma Technical Services re using it in chiller circulators for } } } } } EMs. } } } } } } TechServ_Number: S5525 } } } TechServ_Name: SigmaClean; water bath treatment } } } TechServ_Brand: SIGMA } } } } } } TechServ_re: is this product recommended for use in chiller circulators } } } for } } } the supply of cooling water for a transmission EM (Philips CM10). } } } } } } Best regards, } } } } } } Alastair } } } } } } Alastair McKinnon } } } Histology & EM Facility Manager, IMS R2.62 } } } University of Aberdeen, Institute of Medical Science } } } Foresterhill, Aberdeen, AB25 2ZD } } } tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) } } } fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk } } } www.abdn.ac.uk/ims/h-em } } } -----Original Message----- } } } X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } } } Sent: 10 January 2008 23:00 } } } To: Mckinnon, Alastair D. } } } Subject: [Microscopy] Re: Product for watercooling } } } } } } } } } } } } } } } ------------------------------------------------------------------------ } } } ---- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } } } ---- } } } } } } I would suggest draining the water and replacing with distilled water. } } } The Cl in tap water could lead to corrosion of metal in contact with the } } } water. A good additive IMO is Ethylene Glycol as a 10% mix. I use } } } technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled } } } water for 5 gallons total in the Haskris R050 chiller reservoir. } } } } } } Sometimes this holds up for a year. } } } } } } gary g. } } } } } } } } } } } } At 04:52 AM 1/10/2008, you wrote: } } } } } } } } } } } } } } } } } } } ----------------------------------------------------------------------- } } } } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } } of America To Subscribe/Unsubscribe -- } } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ----------------------------------------------------------------------- } } } } ----- } } } } } } } } Hi! } } } } During some works in a room next to our TEM, one worker opened a } } } } canalisation of the watercooling system, emptying the water tank of the } } } } } } } } watercooling device. } } } } I filled it again with normal tap water but I think I have to add some } } } } product to avoid mold growth. } } } } Could you recommend one such product and where I can order it, } } } } especially those of you from Germany and Austria (we are located in } } } } Vienna, Austria)? } } } } Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM } } } } } } } 1000S. } } } } } } } Thank you in advance, } } } } Stephane } } } } } } } ==============================Original } } } Headers============================== } } } 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- } } } Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net } } } [66.60.130.145]) } } } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id m0AMutZj015411 } } } 11, 20 -- for {microscopy-at-microscopy.com} ; 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} } } 25, 24 -- charset="US-ASCII" } } } 25, 24 -- Subject: RE: [Microscopy] Re: Product for watercooling } } } 25, 24 -- Date: Fri, 11 Jan 2008 15:45:11 -0000 } } } 25, 24 -- Message-ID: } } } {009B2FCFB3DD25408A4BBC240F091376BD20A5-at-VMAIL1.uoa.abdn.ac.uk} } } } 25, 24 -- X-MS-Has-Attach: } } } 25, 24 -- X-MS-TNEF-Correlator: } } } 25, 24 -- Thread-Topic: [Microscopy] Re: Product for watercooling } } } 25, 24 -- thread-index: AchT3EvpoZa+Q4/RS2y35Xsyfvhq1gAi/Eww } } } 25, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} } } } 25, 24 -- To: {gary-at-gaugler.com} } } } 25, 24 -- Cc: {Microscopy-at-microscopy.com} } } } 25, 24 -- Content-Transfer-Encoding: 8bit } } } 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } } ns.microscopy.com id m0BFlgKZ023642 } } } ==============================End of - Headers============================== } } } } } } } } } ==============================Original Headers============================== } } 8, 21 -- From donovan-at-uoregon.edu Fri Jan 11 10:37:25 2008 } } 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) } } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGbOfe005078 } } 8, 21 -- for {microscopy-at-microscopy.com} ; 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If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. } MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 } ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- } } } } } ==============================Original Headers============================== } 5, 20 -- From bbandli-at-mvainc.com Fri Jan 11 10:56:17 2008 } 5, 20 -- Received: from smtp03.atlngahp.sys.nuvox.net (smtp-out3.atlngahp.sys.nuvox.net [70.43.63.20]) } 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGuHYF017808 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:56:17 -0600 } 5, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) } 5, 20 -- by smtp03.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id m0BGuCXC012385 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 11:56:12 -0500 } 5, 20 -- Message-ID: {4787A036.9030005-at-mvainc.com} } 5, 20 -- Date: Fri, 11 Jan 2008 11:58:30 -0500 } 5, 20 -- From: bbandli {bbandli-at-mvainc.com} } 5, 20 -- Reply-To: bbandli-at-mvainc.com } 5, 20 -- Organization: MVA Scientific Consultants } 5, 20 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) } 5, 20 -- MIME-Version: 1.0 } 5, 20 -- To: Microscopy-at-microscopy.com } 5, 20 -- Subject: Re: Product for watercooling } 5, 20 -- References: {200801111637.m0BGbreR006045-at-ns.microscopy.com} } 5, 20 -- In-Reply-To: {200801111637.m0BGbreR006045-at-ns.microscopy.com} } 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 20 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 29 -- From kraftpiano-at-gmail.com Sun Jan 13 12:49:48 2008 3, 29 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0DInlkf028510 3, 29 -- for {microscopy-at-microscopy.com} ; Sun, 13 Jan 2008 12:49:47 -0600 3, 29 -- Received: by rv-out-0910.google.com with SMTP id k20so1660524rvb.30 3, 29 -- for {microscopy-at-microscopy.com} ; Sun, 13 Jan 2008 10:49:46 -0800 (PST) 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 29 -- d=gmail.com; s=gamma; 3, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 29 -- bh=bcp/zJX1peW1n8x2DKC4//HpaWYEo1lQK1S7slKox20=; 3, 29 -- b=SuLr1tQOXEU4RLmIJZj4fnKOALp7Kvqz7QSYrVZdzNdwpa54uEQf/Kjw/R5nf+89isn8wPUMxugW5a12zniVeb/qPjm6oBg1WrAG/Bh7zyv/MUgvHXxk72CsIrDbQTLAAJY053LF/KKKyVk9vr5ho+TcZFqeLYq/VdlIsHqrkuo= 3, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 29 -- d=gmail.com; s=gamma; 3, 29 -- h=message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 29 -- b=AoftEWdLtldcc19ecfiF3e5n7oXQv2FJtSN6La1Ek79iD8crC9bh0geNCN1+KpHj2zvebFB+VScxzHfoFaU3rfisJHVq0dQaziUKWfdXG1YKOhZuAHIjzeCs+7wqtqTzHJi+VwwRnhPH4376xoKqcKeZNdKl1NpRrT8tcV9Ya0k= 3, 29 -- Received: by 10.141.202.12 with SMTP id e12mr3312543rvq.91.1200250186517; 3, 29 -- Sun, 13 Jan 2008 10:49:46 -0800 (PST) 3, 29 -- Received: by 10.140.161.11 with HTTP; Sun, 13 Jan 2008 10:49:46 -0800 (PST) 3, 29 -- Message-ID: {25e2b0d20801131049i7ed2c6c5nc208fec10dbd475b-at-mail.gmail.com} 3, 29 -- Date: Sun, 13 Jan 2008 13:49:46 -0500 3, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 29 -- To: bbandli-at-mvainc.com, microscopy-at-microscopy.com 3, 29 -- Subject: Re: [Microscopy] Re: Product for watercooling 3, 29 -- In-Reply-To: {200801111705.m0BH5RW6027968-at-ns.microscopy.com} 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=ISO-8859-1 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- Content-Disposition: inline 3, 29 -- References: {200801111705.m0BH5RW6027968-at-ns.microscopy.com} ==============================End of - Headers==============================
While chlorine bleach will kill most stuff, at least one chiller manufacturer (Haskris) says that more than ~7ppm free Cl will damage the buna-N O-ring seals. I worked it out a few years ago and figured that it was ~50ml of household bleach in our chiller tank.
We have occasionally done a shock treatment with a high concentration of bleach to kill stuff in the tank, then flushed it out. We've not had any trouble with the seals so far. Minimizing the amount of light that gets to the water makes a real difference in the amount of gunk that develops.
Cheers, Henk
At 01:51 PM 1/13/2008, kraftpiano-at-gmail.com wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility (614) 292-0674 040 Fontana Labs, 116 W. 19th Ave www.ceof.ohio-state.edu
==============================Original Headers============================== 11, 26 -- From colijn.1-at-osu.edu Sun Jan 13 14:04:17 2008 11, 26 -- Received: from er6s1.ecr6.ohio-state.edu (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0DK4Hx2010488 11, 26 -- for {microscopy-at-microscopy.com} ; Sun, 13 Jan 2008 14:04:17 -0600 11, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 11, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31056) 11, 26 -- id {01MQ1RHJAGTSACU47A-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 11, 26 -- Sun, 13 Jan 2008 15:04:16 -0500 (EST) 11, 26 -- Received: from HOC3.osu.edu 11, 26 -- (d118-75-136-133.try.wideopenwest.com [75.118.133.136]) 11, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31056) 11, 26 -- with ESMTPA id {01MQ1RHI06OUACH3MM-at-ecr6.ohio-state.edu} ; Sun, 11, 26 -- 13 Jan 2008 15:04:16 -0500 (EST) 11, 26 -- Date: Sun, 13 Jan 2008 15:01:41 -0500 11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 26 -- Subject: Re: [Microscopy] Product for watercooling 11, 26 -- In-reply-to: {200801131851.m0DIpgXQ030978-at-ns.microscopy.com} 11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 26 -- To: kraftpiano-at-gmail.com 11, 26 -- Cc: microscopy-at-microscopy.com 11, 26 -- Message-id: {7.0.1.0.2.20080113144228.027d52c8-at-osu.edu} 11, 26 -- MIME-version: 1.0 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 26 -- References: {200801131851.m0DIpgXQ030978-at-ns.microscopy.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both PRICE-at-gw.med.sc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: PRICE-at-gw.med.sc.edu Name: Bob Price
Organization: USC- School of Medicine
Title-Subject: [Filtered] Basic Confocal Microscopy and Digital Imaging Workshop
Question: Dear List Members,
Appended below is information about our fourth Basic Confocal Microscopy and Digital Imaging Workshop that will be held at the University of South Carolina School of Medicine from June 16-20, 2008.
Workshop on Basic Confocal Microscopy and Digital Imaging: Brief Synopsis
The South Carolina EPSCoR/IDeA Program and the USC School of Medicine Instrumentation Resource Facility are pleased to announce the 4th annual workshop on Basic Confocal Microscopy and Digital Imaging. The hands-on workshop will target beginning and intermediate users of confocal microscopes and will provide lectures from experts in the field of confocal microscopy and the use of Adobe Photoshop and 3-D software for processing of confocal images. Lecture material will provide information on the basics of fluorescence and fluorescent probes, biological specimen preparation (fixation, staining, optical properties and mounting materials), strategies and protocols for selection of antibody labeling, the basic components of a confocal microscope (lasers, dichroic mirrors, microscope objectives, photomultiplier tubes, etc.) and an overview of some applications of confocal microscopy.
During the laboratory portion of the workshop specimens will be processed for double and triple labeling and proper selection of user adjustable parameters to optimize image collection will be addressed and demonstrated. Participants are welcome to process their own samples or to use samples that will be provided. Several point scanning and spinning disk confocal systems from various manufacturers will be available for use so participants will have ample time for hands on use of the instruments during the workshop.
Faculty scheduled to participate include:
Dr. Bob Price, Research Professor, Cell and Developmental Biology and Anatomy, and Director, Instrumentation Resource Facility, University of South Carolina School of Medicine (http://dba.med.sc.edu/price/irf/irf.htm) Dr. Jay Jerome, Associate Professor, Pathology and Cancer Biology, Vanderbilt University (https://medschool.mc.vanderbilt.edu/facultydata/php_files/show_faculty.php?id3=1032) Dr. Ralph Albrecht, Professor, Department of Animal Sciences, University of Wisconsin-Madison (http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/index.htm) Dr. John Mackenzie, Professor, Microbiology, North Carolina State University (http://www.ncsu.edu/cem/index.html) Dr. Tom Trusk, Associate Professor, Department of Cell Biology and Anatomy, Medical University of South Carolina (http://cba.musc.edu/faculty/TruskT.htm)
Past workshops have been very successful with over 50 attendees at each. For further information contact Bob Price (Price-at-med.sc.edu) or visit the workshop website: http://dba.med.sc.edu/price/irf/irf.htm
Robert L. Price, PhD Research Professor and Director, Instrumentation Resource Facility School of Medicine, USC Bldg 1 Room B60 6439 Garner's Ferry Road Columbia, SC 29208
Perhaps it would comfort you to know that I have exactly the same feeling with our Zeiss axiovert. The microscope itself is well designed but the software is user-unfriendly. The main operations, those obligatory steps that you use all the time are dispersed in different commands and subcommands. I constantly feel that I am using only 0,1% of the possibilities of the system, without hope of increasing this percentage. The best answer I can give you is to arrange a course with Zeiss.
Best regards, Stephane
PS: Z-stack on bacteria with a light microscope?? Are you sure you are not talking about confocal?
----- Original Message ---- X-from: "alissa.susanne-at-gmail.com" {alissa.susanne-at-gmail.com} To: nizets2-at-yahoo.com Sent: Saturday, January 12, 2008 12:38:35 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alissa.susanne-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: alissa.susanne-at-gmail.com Name: Alissa H
Organization: University of Maryland
Title-Subject: [Filtered] specimen preparation
Question: We have a new Zeiss AxioImager here, and I've been given the task of not only learning how to use it, but also one of my main projects involves use of the microscope to evaluate fluorescence of some bacteria. I've transformed a plasmid with YFP on it into some streptococcus. I can find the buggers, take a reasonable Z stack, and even deconvolve the image.
What I'd like to learn is better ways to prepare my samples, right now I've just been making wet mounts with saline. I'd also like to learn how to better use Zeiss' computer program that we got with the microscope. I don't find the program intuitive, like I do for most computer programs, and am getting frustrated. If anyone can give me good suggestions on where I can read about these things, I'd really appreciate it. I feel like a total newbie, and I'm not quite sure where to go to get good information.
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Fri Jan 11 17:31:25 2008 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BNVO20012072 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 17:31:25 -0600 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p06240803c3adacbe9638-at-[206.69.208.22]} 8, 11 -- Date: Fri, 11 Jan 2008 17:31:23 -0600 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: alissa.susanne-at-gmail.com (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: specimen preparation 8, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 20, 20 -- From nizets2-at-yahoo.com Mon Jan 14 08:11:50 2008 20, 20 -- Received: from web37413.mail.mud.yahoo.com (web37413.mail.mud.yahoo.com [209.191.91.145]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0EEBoZ5002890 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jan 2008 08:11:50 -0600 20, 20 -- Received: (qmail 52176 invoked by uid 60001); 14 Jan 2008 14:11:49 -0000 20, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 20 -- s=s1024; d=yahoo.com; 20, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 20, 20 -- b=6FI8gNzwtHWOpPeRmsrj+Kr2cwszebzN6qHTZJgP2U9qV0/6wLXogGBSkFxpes+oCYSNMRY8dnCrcgb2ILcPSDGu7wdBcko+hYb0NJY3WmfJvAeBMFinINIaEdQ2LMmtIshVXAzR6mCKXABFVVIeWRZIjb5kDfEWDpRvXd0d/VA=; 20, 20 -- X-YMail-OSG: qaADhCQVM1mMhSXYkNxinWbgAKMDmxo6WBuogdroqI.c7TkpIZoGpeIlVKFiEMVnWjjGs4x12FVdu_6LqTuaN_XGJG9IjdNDAgjXDnWa.QyzpMBKD8JExrg8uWlltpR1OkAqqnc3XAuqSyyeGc5PIKkIuRETqzD8Hfas.Q60lHW0 20, 20 -- Received: from [80.122.101.100] by web37413.mail.mud.yahoo.com via HTTP; Mon, 14 Jan 2008 06:11:49 PST 20, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.160 20, 20 -- Date: Mon, 14 Jan 2008 06:11:49 -0800 (PST) 20, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 20, 20 -- Subject: Re: [Microscopy] viaWWW: specimen preparation 20, 20 -- To: alissa.susanne-at-gmail.com 20, 20 -- Cc: microscopy-at-microscopy.com 20, 20 -- MIME-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=us-ascii 20, 20 -- Message-ID: {771437.52040.qm-at-web37413.mail.mud.yahoo.com} ==============================End of - Headers==============================
I agree about the opaque lines but found it much easier to use normal transparent hoses and wrap them in aluminium foil with a bit of tape. It's worked for years on our SEM.
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: bbandli-at-mvainc.com
Preventing the growth of algae in cooling water systems is discussed in detail in my book "Vacuum Methods in Electron Microscopy" Two chemicals commonly used to prevent algal growth in such systems are Chloramine T and dichlorophene. Both of these chemicals can be obtained from various specialty chemical companies. You can also probably obtain algaecides from companies (and merchants) that sell water beds, and swimming pool equipment. I do not recommend the use of ethylene glycol for several reasons that are discussed in my book. Also, remember that algae require light in order to grow, and so you can substantially inhibit their growth by fully excluding light from the system (i.e. cover the reservoir with a a light-tight cover, and use fully opaque tubing). Changing from ordinary tap water to distilled water will probably not give you much of an advantage, except for possibly minimizing the formation of a bit of scale in the heated parts of the diffusion pump. However, the amount of scale formation should be quite limited since it is a closed system containing a limited amount of water. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Mon Jan 14 15:19:40 2008 1, 14 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0ELJed0012094 1, 14 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jan 2008 15:19:40 -0600 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY skycaptain.mr.itd.umich.edu ID 478BD1EA.336C2.17443 ; 1, 14 -- 14 Jan 2008 16:19:38 -0500 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240800c3b17f44c5a5-at-[141.212.131.221]} 1, 14 -- Date: Mon, 14 Jan 2008 16:19:34 -0500 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: Algaecides 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slc6-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh Microscopy School
Title-Subject: [Filtered] Lehigh Microscopy School Courses
Question: There is still time to register for the 2008 Lehigh Microscopy School which will be held June 1-13, 2008. This will be the 38th year of course offerings which include:
SEM and X-Ray Microanalaysis (June 2-6)
Introduction to SEM and EDS for the New Operator (June 1)
Scanning Probe Microscopy: From Fundamentals to Advanced Applications (June 9-12)
Problem Solving with SEM, X-ray Microanalysis, and Electron Backscatter Patterns (June 9-13)
Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques (June 9-13)
Analytical Electron Microscopy at the Nanometer Scale (June 9-12)
Focused Ion Beam (FIB) Instrumentation and Applications (June 9-12)
Complete course descriptions and registration form are available at www.Lehigh.edu/microscopy. Contact Sharon Coe (Sharon.coe-at-Lehigh.edu) for more information.
Has anyone encountered problems with fumes escaping from specimen chamber of a Leica Freeze substitution unit ( AFS not AFS2.) during fluid changes.
A new user has noticed, especially when doing changes with Lowicryl HM20, that the fumes are escaping and therefore it is putting the user at risk and others who may be in the area at the time.The built in venting system of the AFS is ducted into a fume hood and appears to be working, at least we can feel air movement from it. Is it possible the fan is not working efficiently ?
In the short term, though not ideal, we are dealing with this by providing users with suitable respirator masks. A more long term/safer solution we are looking into is to have some sort of extract system that will draw fumes away from the user.
If you have encountered this problem before how did you overcome it?
Christine Richardson.
School of Biological & Biomedical Science Centre for Molecular Imaging University of Durham UK.
==============================Original Headers============================== 8, 28 -- From a.c.richardson-at-durham.ac.uk Tue Jan 15 07:18:37 2008 8, 28 -- Received: from hermes1.dur.ac.uk (hermes1.dur.ac.uk [129.234.8.20]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0FDIaBk008790 8, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jan 2008 07:18:37 -0600 8, 28 -- Received: from smtphost4.dur.ac.uk (smtphost4.dur.ac.uk [129.234.4.18]) 8, 28 -- by hermes1.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m0FDINM9008765 8, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jan 2008 13:18:28 GMT 8, 28 -- Received: from weaver.dur.ac.uk (weaver.dur.ac.uk [129.234.4.167]) 8, 28 -- by smtphost4.dur.ac.uk (8.13.7/8.13.7) with ESMTP id m0FDIMVp002984 8, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jan 2008 13:18:22 GMT 8, 28 -- Received: (from httpd-at-localhost) 8, 28 -- by weaver.dur.ac.uk (8.11.7p3+Sun/8.11.1) id m0FDIMX00491 8, 28 -- for Microscopy-at-microscopy.com; Tue, 15 Jan 2008 13:18:22 GMT 8, 28 -- X-Authentication-Warning: weaver.dur.ac.uk: httpd set sender to dbl0acr-at-smtphost.dur.ac.uk using -f 8, 28 -- Received: from biosci-71.dur.ac.uk (biosci-71.dur.ac.uk [129.234.133.71]) 8, 28 -- by webmailimpa.dur.ac.uk (IMP) with HTTP 8, 28 -- for {dbl0acr-at-imaphost.dur.ac.uk} ; Tue, 15 Jan 2008 13:18:22 +0000 8, 28 -- Message-ID: {1200403102.478cb29e3f019-at-webmailimpa.dur.ac.uk} 8, 28 -- Date: Tue, 15 Jan 2008 13:18:22 +0000 8, 28 -- From: a.c.richardson-at-durham.ac.uk 8, 28 -- To: Microscopy-at-microscopy.com 8, 28 -- Subject: TEM : AFS problem 8, 28 -- MIME-Version: 1.0 8, 28 -- Content-Type: text/plain; charset=ISO-8859-1 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.1 8, 28 -- X-Originating-IP: 129.234.133.71 8, 28 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
here is a description of the position. Please contact me for further inquiries.
Thanks and best regards,
Loic Vilde
Failure Analysis Engineer
Description :
You will apply your scientific and analytical skills to assist in improving product reliability through methodical root cause analysis of failed products.
Your responsibilities will include to design, plan and lead all the investigations necessary to identify the root cause of failed products (field returns or test samples). You will approve, archive and distribute the associated documentation.
You will also work on developing and implementing new investigative procedures specifically aimed at our designs and on organizing failure analysis conclusions in a knowledge database. Our products use diverse materials, from thermoplastics to Titanium parts, with a special emphasis on microelectronics components and printed circuit boards.
You will interface with the Product Development Team members and Manufacturing engineers, as well as with other Environmental Qualification engineers and technical experts from other Schlumberger Technology Centres, and external failure analysis labs.
Equipment used onsite to perform these investigations includes: cold mounting station, dye, precision cut-off machine, semi-automatic grinder/polisher, carbon coater, optical microscopes, variable pressure scanning electron microscope (VP-SEM) with energy-dispersive X-Ray microanalysis (EDS) and X-ray inspection system (Non-Destructive Testing).
Technical, product development, quality management and business related training will be provided.
Notes : International Candidates Will Be Considered. Employer will assist with relocation costs
Requirements :
Education and Qualifications: * PhD in Material Science or MSc with working experience in Failure Analysis domain * Minimum 3 years working experience in similar field * English (working language) Expertise in one or several of the below domains will be highly appreciated : * Scientific/technical experimentation and methods * Analytical investigation procedures, especially sample preparation for SEM/EDS analysis of microelectronics components and electronic assemblies Others : * Ability to organize and document investigations * Strong SEM/EDS and sample preparation skills * Team player with open and direct communication style * Solid computer skills/literacy, especially in data analysis * Effective oral and written communication skills Employer :
WesternGeco, the world's largest geophysical services company, provides comprehensive worldwide reservoir imaging, monitoring, and development services, with the most extensive geophysical survey crews and data processing centers in the industry, as well as the world's largest multiclient data library. Services range from 3D and time-lapse (4D) seismic surveys to multicomponent surveys for delineating prospects and reservoir management as well as electromagnetic surveys.
Loïc VILDE Environmental Qualification / Test and Integration Manager ------------------------------------------------------------- WesternGeco - Oslo Technology Center Schlumberger House Solbråveien 23 N-1372 Asker – Norway
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckee-at-helix.mgh.harvard.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] service for microtomes
Question: Thanks to everyone who responded to my question. You are great!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sandeep-at-frontedgetechnology.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Scanning Electron Microscope Repair & Maintenance
Question: Hello All,
We require repair and maintenance services for the SEM & X-ray detector. Please recommend the service providers. We are located in Southern California.
One of my users has been doing some subcellular fractionation of plant cells, and would like to look at the fractions to see if we can identify specific organelles. Are there any specific probes that will help us? I would appreciate any advice! We do have a confocal microscope, which should give us better resolution than a std fluorescence microscope.
Thanks in advance
shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1760 Facsimile/Télécopieur: 613-759-1701 Rm 2068 K.W. Neatby Bldg 960 Carling Ave. Central Experimental Farm Ottawa, ON K1A 0C6 millers-at-agr.gc.ca
==============================Original Headers============================== 10, 26 -- From MILLERS-at-AGR.GC.CA Wed Jan 16 15:07:25 2008 10, 26 -- Received: from agrpazsmtp1.agr.gc.ca (agrpazsmtp1.agr.gc.ca [192.197.71.31]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0GL7OT1009056 10, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Jan 2008 15:07:24 -0600 10, 26 -- Received: from onottaxsmtp2.AGR.GC.CA ([10.117.12.122]) 10, 26 -- by agrpazsmtp1.agr.gc.ca (8.13.6/8.13.3) with ESMTP id m0GL7ODl028085 10, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Jan 2008 16:07:24 -0500 10, 26 -- Received: from ONOTTAXMS2.AGR.GC.CA ([10.117.12.102]) by onottaxsmtp2.AGR.GC.CA with Microsoft SMTPSVC(6.0.3790.3959); 10, 26 -- Wed, 16 Jan 2008 16:07:24 -0500 10, 26 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 10, 26 -- Content-class: urn:content-classes:message 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; 10, 26 -- charset="iso-8859-1" 10, 26 -- Subject: LM/Confocal- need help identifying plant organelles 10, 26 -- Date: Wed, 16 Jan 2008 16:07:24 -0500 10, 26 -- Message-ID: {FD493497DF264A4E8DC71F1FFE49129D068C69-at-ONOTTAXMS2.AGR.GC.CA} 10, 26 -- X-MS-Has-Attach: 10, 26 -- X-MS-TNEF-Correlator: 10, 26 -- Thread-Topic: LM/Confocal- need help identifying plant organelles 10, 26 -- Thread-Index: AchYg8mtFSj+TLMSTEuQ2ADiaPNl8A== 10, 26 -- From: "Miller, Shea" {MILLERS-at-AGR.GC.CA} 10, 26 -- To: {Microscopy-at-Microscopy.Com} 10, 26 -- X-OriginalArrivalTime: 16 Jan 2008 21:07:24.0256 (UTC) FILETIME=[CA919E00:01C85883] 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0GL7OT1009056 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jason_jla-at-hotmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 16, 2008 at 11:53:09 ---------------------------------------------------------------------------
Email: jason_jla-at-hotmail.com Name: Jason Alexander
Organization: Immanuel-St. James
Education: K-8 Grade Grammar School
Location: Grand Rapids, MI
Question: Where can I purchase a good microscope that can be used for CELLS and MICROSTRUCTURES OF METALLIC SPECIMENS? I am writing a grant for a microscope and the organization recommended I ask you this question. Thank you for your time?
==============================Original Headers============================== 7, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Jan 16 19:04:36 2008 7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0H14Zlu028839 7, 12 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jan 2008 19:04:36 -0600 7, 12 -- Mime-Version: 1.0 7, 12 -- Message-Id: {p06240801c3b45a0b3cb3-at-[206.69.208.22]} 7, 12 -- Date: Wed, 16 Jan 2008 19:04:34 -0600 7, 12 -- To: microscopy-at-microscopy.com 7, 12 -- From: jason_jla-at-hotmail.com (by way of Ask-A-Microscopist) 7, 12 -- Subject: AskAMicroscopist: where to purchase a good microscope that can 7, 12 -- be used for CELLS and MICROSTRUCTURES 7, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (john.d.williams-at-colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 16, 2008 at 12:26:42 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both john.d.williams-at-colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: john.d.williams-at-colostate.edu Name: Prof. John D. Williams
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Title: Questions on an ISI-DS130S
Question: I'm being offered a scanning microscope as a donation. It is an ISI-DS130S and was in good working order 2 years ago before being crated up. Does anyone have any detailed information on this scope? I just need a good workhorse for inspecting heavily ion etched surfaces. The last significant contact I've had with an SEM was with Bob Lee at CSU in the late 1980s. So everyone should consider me a newbie. Thanks.
This Question was submitted to Ask-A-Microscopist by (john.d.williams-at-colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 16, 2008 at 22:03:38 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both john.d.williams-at-colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: john.d.williams-at-colostate.edu Name: Prof. John D. Williams
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Title: ISI-DS130S
Question: Does anyone have an ISI-DS130S operating/maintenance manual they would be willing sell/give me? Thanks, John Williams
Follow-up: I am grateful to Maryah Converse, daughter of Ken Converse of www.qualityimages.biz for translating the descriptions of the images of my SEM calendar "Microstructures 2008". All texts on page 14 are now in English language.
If anybody is able to put the complete calendar PDF www.elektronenmikroskopie.info/calendar_2008.pdf (39 MB) on a server with unrestricted traffic, please do so and let the list know. I will take the file off my server within 12 hours, because I spend all my free transfer volume for this month on my server ;-( If you would only like to get the updated page 14 with the English text, this is the place: www.elektronenmikroskopie.info/calendar_2008_p14.pdf (350 KB)
Best regards, Stefan
} } Dear All, } thanks for the good discussion and the helpful tips to all on the list. } } } With the best wishes for a } happy new year 2008, } success at work and in business, } } Stefan Diller } } } } Please feel free to download and print out my new SEM Calendar 2008 } with images from scanning electron microscopy: } www.elektronenmikroskopie.info/calendar_2008.pdf (39 MB). Images are } Copyright Stefan Diller 2008, please ask for permission prior to any } commercial use. }
Hi all, Happy New Year! Does anyone have experience thick-sectioning watermelon seeds? We're working with mature seeds - black seed coat - unfixed, not embedded. The seeds are left in a moist chamber overnight to imbibe water. Vibratome sectioning didn't work well. Any suggestions? Would fixing and embedding make them easier to section?
I think they're really only good for spitting...but the researcher doesn't want to hear that;-) Any help would be greatly appreciated. Many thanks, Beth
Beth Richardson Plant Biology Department University of Georgia Athens, GA 30602 706-542-1790
==============================Original Headers============================== 4, 19 -- From beth-at-plantbio.uga.edu Thu Jan 17 10:21:22 2008 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGLLmX009715 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:21:21 -0600 4, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) 4, 19 -- by dogwood.plantbio.uga.edu 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 4, 19 -- for microscopy-at-microscopy.com; 4, 19 -- Thu, 17 Jan 2008 11:21:15 -0500 4, 19 -- Message-Id: {80AFBBC9-F649-4422-84BA-331CE918C9EB-at-plantbio.uga.edu} 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) 4, 19 -- Subject: sectioning watermelon seeds 4, 19 -- Date: Thu, 17 Jan 2008 11:22:05 -0500 4, 19 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
Hello All, I have a researcher who wants to look at polyurethane coated wires with the SEM. His goal is to see any cracks, breaks or defects in the metal wire encased by the polyurethane. These are very tiny wires, he cannot remove the covering and he needs to look at the wire in sections for defects. We have a super SEM with a backscatter detector but no carbon coater. Any one have experience with this type of sample? How can I prep the samples for the SEM?? Any suggestions would be greatly appreciated!! Thanks! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
==============================Original Headers============================== 3, 21 -- From pekysar-at-ucdavis.edu Thu Jan 17 10:28:28 2008 3, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGSRFu017857 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:28:28 -0600 3, 21 -- Received: from mpat3317 ([169.237.197.34]) 3, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with SMTP id m0HGSQoB007412 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 08:28:27 -0800 (PST) 3, 21 -- Message-ID: {001701c85926$549f8970$22c5eda9-at-ucdsom.ucdavis.edu} 3, 21 -- From: "Pat Kysar" {pekysar-at-ucdavis.edu} 3, 21 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 21 -- Subject: SEM of Wires Encased in Polyurethane 3, 21 -- Date: Thu, 17 Jan 2008 08:30:54 -0800 3, 21 -- MIME-Version: 1.0 3, 21 -- Content-Type: text/plain; 3, 21 -- charset="iso-8859-1" 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-Priority: 3 3, 21 -- X-MSMail-Priority: Normal 3, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1914 3, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1914 3, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 ==============================End of - Headers==============================
Dear All, thanks to Kay at www.quantifoil.com at Jena, Germany the calendar PDF finally moved on to this download address: www.quantifoil.com/calendar_2008.pdf (38 MB) It will be available hopefully the rest of 2008.
If you would only like to get the updated page 14 with the English text, it will stay available here: www.elektronenmikroskopie.info/calendar_2008_p14.pdf (350 KB)
Visit the Geology Department at Davis. They will have a carbon coater.
On Jan 17, 2008, at 11:28 AM, pekysar-at-ucdavis.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello All, } I have a researcher who wants to look at polyurethane coated wires } with the } SEM. His goal is to see any cracks, breaks or defects in the metal } wire } encased by the polyurethane. These are very tiny wires, he cannot } remove the } covering and he needs to look at the wire in sections for defects. } We have a } super SEM with a backscatter detector but no carbon coater. Any one } have } experience with this type of sample? How can I prep the samples for } the } SEM?? Any suggestions would be greatly appreciated!! Thanks! } Pat Kysar } University of California, Davis } Medical School, Pathology } EM Lab } } ---------------------------------
Gordon L. Nord, Ph. D Senior Scientist International Environmental Research Foundation New York, NY
{http://www.ierfinc.org/}
==============================Original Headers============================== 5, 24 -- From gnord-at-mindspring.com Thu Jan 17 11:19:54 2008 5, 24 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HHJsFl009275 5, 24 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 17 Jan 2008 11:19:54 -0600 5, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 24 -- s=dk20050327; d=mindspring.com; 5, 24 -- b=sqpdoPTjE2p9akbrDPm5qWkCegFk57dghjW7lK6jTZszqv/ywq3qOM4hA51VyPIF; 5, 24 -- h=Received:Mime-Version:In-Reply-To:References:Content-Type:Message-Id:Content-Transfer-Encoding:From:Subject:Date:To:X-Mailer:X-ELNK-Trace:X-Originating-IP; 5, 24 -- Received: from [71.171.97.30] (helo=[10.0.1.198]) 5, 24 -- by elasmtp-mealy.atl.sa.earthlink.net with asmtp (Exim 4.34) 5, 24 -- id 1JFYPB-0003Pn-L2; Thu, 17 Jan 2008 12:19:53 -0500 5, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 24 -- In-Reply-To: {200801171628.m0HGSoN1019030-at-ns.microscopy.com} 5, 24 -- References: {200801171628.m0HGSoN1019030-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 24 -- Message-Id: {3B5CEB15-5891-4CC9-9762-AF690DE41DB5-at-mindspring.com} 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- From: Gordon Nord {gnord-at-mindspring.com} 5, 24 -- Subject: Re: [Microscopy] SEM of Wires Encased in Polyurethane 5, 24 -- Date: Thu, 17 Jan 2008 12:19:52 -0500 5, 24 -- To: pekysar-at-ucdavis.edu, Microscopy-at-MSA.Microscopy.com 5, 24 -- X-Mailer: Apple Mail (2.753) 5, 24 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d8885d2a9c731cc891172964ec30db4ae644ef813bcc8d13dfc1350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 5, 24 -- X-Originating-IP: 71.171.97.30 ==============================End of - Headers==============================
Greetings, This is the first announcement for students who will attend the Microscopy and Microanalysis Meeting in Albuquerque, Aug.3-7 and would like to apply for a Student Bursary to help defray meeting costs. The complete description of the Bursary is listed below, including the registration process.
The contact person this year that will coordinate the initial student bursary sign-up is: Amanda Lawrence (alawrence-at-entomology.msstate.edu ). Clayton Lohen (clohen-at-vt.edu) will be helping with coordination of bursary activities once on site.
STUDENT BURSARIES The Microscopy Society of America values student members and recognizes that they are the future of both the society and the field of microscopy in general. MSA is therefore pleased to offer student bursaries of $200 to registered students. The most important purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where these young scientists can meet and interact with the established microscopy community as well as assisting with the meeting.
Each bursary recipient will be expected to work for a minimum of 20 hours during the meeting and/or at the pre-meeting weekend events. A student may work up to an additional 20 hours, for a total of 40 hours. These extra hours will add to the bursary total at a rate of $10 per hour. The maximum bursary will therefore be $400. The duties will involve, but are not necessarily limited to, providing support in symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth, and monitoring use of the Internet Cafe. Applicants for the bursaries must be members of MSA or MAS, and enrolled as students at a recognized educational institution. All MSA or MAS student members are eligible for bursaries, including those who are recipients of MSA Presidential Scholars Awards and MAS Distinguished Scholar Awards. Eligible on the conference website, or at on-site registration. Bursaries are limited and early application is encouraged. Don't forget to check the website for special student housing discounts as well.
How it works: The registration form for the meeting will have a check box indicating that the applicant is a registered student and is requesting a bursary. The check box will have a note beside it reminding the applicant that the bursary requires them to work at the meeting.
Students who have applied for bursaries will be contacted by the initial student bursary co-coordinator (Amanda) to schedule meeting tasks prior to arrival in Albuquerque. The student is then expected to fulfill their assigned tasks and will be issued forms which must be signed by all of the contact persons listed, indicating that all assigned tasks have been performed. Upon completion of assignments and submission of the signed forms, the bursary check will be issued by the appropriate LAC representative.
We would also like to solicit volunteers from 'non-students' as well to help with meeting activities.
Should you have questions , please contact:
Amanda Lawrence (alawrence-at-entomology.msstate.edu) Electron Microscope Center 100 Twelve Lane Mississippi State University Mississippi State, MS 39762 (662)-325-3019 Work (662)-325-0246 Fax
==============================Original Headers============================== 13, 20 -- From ALawrence-at-entomology.msstate.edu Thu Jan 17 13:14:13 2008 13, 20 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HJECAA000748 13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 13:14:13 -0600 13, 20 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 13, 20 -- with Novell_GroupWise; Thu, 17 Jan 2008 13:14:11 -0600 13, 20 -- Message-Id: {478F549A.B26A.00E6.0-at-entomology.msstate.edu} 13, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 13, 20 -- Date: Thu, 17 Jan 2008 13:14:02 -0600 13, 20 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 13, 20 -- To: {microscopy-at-microscopy.com} 13, 20 -- Subject: M&M 2008 student bursary and volunteer request 13, 20 -- References: {478F515B020000E6000314B8-at-mailhost2.groupwise.msstate.edu} 13, 20 -- {478F520B020000E6000314BB-at-mailhost2.groupwise.msstate.edu} 13, 20 -- {478F549A020000E6000314BE-at-mailhost2.groupwise.msstate.edu} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=US-ASCII 13, 20 -- Content-Disposition: inline 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0HJECAA000748 ==============================End of - Headers==============================
I have several questions that may allow you to provide more specific information useful to the listserver community: Is the polyurethane cross-linked? Does he need to examine the wire in cross-section or might examination of the surface of the wire suffice? If a cross-section is required, which plane of the wire does he want to examine, i.e. the axial cross-section or the longitudinal cross-section? How do you intend to prepare the cross-sections: grinding and polishing; microtomy; FIB, etc?
If the polyurethane is not cross-linked, I suggest you consider dissolving it in a good solvent, thus leaving the bare wires available for subsequent sample preparation and microscopy.
Best regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
gnord-at-mindspri ng.com To gary.m.brown-at-exxonmobil.com 01/17/08 11:21 cc AM Subject [Microscopy] Re: SEM of Wires Please respond Encased in Polyurethane to gnord-at-mindspri ng.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Visit the Geology Department at Davis. They will have a carbon coater.
On Jan 17, 2008, at 11:28 AM, pekysar-at-ucdavis.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello All, } I have a researcher who wants to look at polyurethane coated wires } with the } SEM. His goal is to see any cracks, breaks or defects in the metal } wire } encased by the polyurethane. These are very tiny wires, he cannot } remove the } covering and he needs to look at the wire in sections for defects. } We have a } super SEM with a backscatter detector but no carbon coater. Any one } have } experience with this type of sample? How can I prep the samples for } the } SEM?? Any suggestions would be greatly appreciated!! Thanks! } Pat Kysar } University of California, Davis } Medical School, Pathology } EM Lab } } ---------------------------------
Gordon L. Nord, Ph. D Senior Scientist International Environmental Research Foundation New York, NY
{http://www.ierfinc.org/}
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==============================Original Headers============================== 33, 19 -- From gary.m.brown-at-exxonmobil.com Thu Jan 17 14:42:52 2008 33, 19 -- Received: from dalspc01.exxonmobil.com (dalspc01.exxonmobil.com [131.126.223.1]) 33, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HKgohL016608 33, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 14:42:51 -0600 33, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 33, 19 -- by dalspc01.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m0HKgmo7011902; 33, 19 -- Thu, 17 Jan 2008 14:42:48 -0600 (CST) 33, 19 -- In-Reply-To: {200801171721.m0HHLh8q015030-at-ns.microscopy.com} 33, 19 -- Subject: Re: [Microscopy] Re: SEM of Wires Encased in Polyurethane 33, 19 -- Importance: 33, 19 -- To: gnord-at-mindspring.com, microscopy-at-microscopy.com 33, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 33, 19 -- Message-ID: {OF7191A216.E3A105D0-ON862573D3.006F4302-862573D3.0071C2DC-at-exxonmobil.com} 33, 19 -- From: gary.m.brown-at-exxonmobil.com 33, 19 -- Date: Thu, 17 Jan 2008 14:42:34 -0600 33, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 33, 19 -- 02, 2006) at 01/17/2008 02:42:48 PM 33, 19 -- MIME-Version: 1.0 33, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgengle-at-uky.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mgengle-at-uky.edu Name: Mary Gail Engle
Organization: Imaging Facility, University of Kentucky
Title-Subject: [Filtered] Microwave processing for TEM
Question: Having just attended a microwave workshop and being impressed with the morphology of the tissue for "normal" EM, I was wondering if any of you have experience with, or an opinion regarding the quality of colloidal gold immunolabeling using the microwave. Speed is not necessarily an issue for us, but if we could get better morphology as well as good gold labeling, we would consider purchasing the instrument.
Thank you, Mary Gail Engle, Manager, Imaging Facility University of KY
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Email: atn5613-at-rit.edu Name: Algis N
Organization: Rochester Institute of Technology
Title-Subject: [Filtered] Target glue for PSD
Question: Hello All, I am doing some research using a plasma sputtering device (VCR Group IBS TM200S)to sputter metals onto a substrate. What type of adhesive should I use to adhere the metal foil targets I will be using to the target holder? Should it be conductive and what contamination concerns should I be looking for? Thanks. Algis
Pat, I'm a little confused by "he needs to look at the wire in sections". If you mean that the wire will be cross-sectioned, then advice has already been given: mount, grind and polish. If, on the other hand, you mean that short, perhaps specific, sections will be examined for surface defects on the metal wire and "he cannot remove the covering", my question is: how thick is the polyurethane insulation? If it's only a couple of microns thick, try going to your maximum kV and see if the beam will penetrate the insulation. BSE may give the best image, but SE might also work. If the insulation is too thick for beam penetration, you're left with either stripping or sectioning.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: pekysar-at-ucdavis.edu [mailto:pekysar-at-ucdavis.edu] Sent: Thursday, January 17, 2008 11:31 AM To: kenconverse-at-qualityimages.biz
Hello All, I have a researcher who wants to look at polyurethane coated wires with the SEM. His goal is to see any cracks, breaks or defects in the metal wire encased by the polyurethane. These are very tiny wires, he cannot remove the covering and he needs to look at the wire in sections for defects. We have a super SEM with a backscatter detector but no carbon coater. Any one have experience with this type of sample? How can I prep the samples for the SEM?? Any suggestions would be greatly appreciated!! Thanks! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
==============================Original Headers============================== 3, 21 -- From pekysar-at-ucdavis.edu Thu Jan 17 10:28:28 2008 3, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGSRFu017857 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:28:28 -0600 3, 21 -- Received: from mpat3317 ([169.237.197.34]) 3, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with SMTP id m0HGSQoB007412 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 08:28:27 -0800 (PST) 3, 21 -- Message-ID: {001701c85926$549f8970$22c5eda9-at-ucdsom.ucdavis.edu} 3, 21 -- From: "Pat Kysar" {pekysar-at-ucdavis.edu} 3, 21 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 21 --
Ken,
Unfortunately, many technologists are put in your position. That is, one may be asked to analyze potentially complex, multi-component materials without substantive guidance from the requestor, in areas outside one's expertise, and with little help within our immediate technical community.
I encourage you to put some of the responsibility back onto the requestor. He/she should be able to obtain information on the composition of the polyurethane insulation from the manufacturer. If the requestor is not willing to spend any time or effort to work the problem, just how important can it be? The last point I would make regards the scope of your technical capabilities. Specifically, do you have the sample preparation and microscopy capabilities that will be needed in the analysis of this material.
Best regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
kenconverse-at-qu alityimages.bi z To gary.m.brown-at-exxonmobil.com cc 01/18/08 02:55 PM Subject [Microscopy] RE: SEM of Wires Encased in Polyurethane Please respond to kenconverse-at-qu alityimages.bi z
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Pat, I'm a little confused by "he needs to look at the wire in sections". If you mean that the wire will be cross-sectioned, then advice has already been given: mount, grind and polish. If, on the other hand, you mean that short, perhaps specific, sections will be examined for surface defects on the metal wire and "he cannot remove the covering", my question is: how thick is the polyurethane insulation? If it's only a couple of microns thick, try going to your maximum kV and see if the beam will penetrate the insulation. BSE may give the best image, but SE might also work. If the insulation is too thick for beam penetration, you're left with either stripping or sectioning.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: pekysar-at-ucdavis.edu [mailto:pekysar-at-ucdavis.edu] Sent: Thursday, January 17, 2008 11:31 AM To: kenconverse-at-qualityimages.biz
Hello All, I have a researcher who wants to look at polyurethane coated wires with the SEM. His goal is to see any cracks, breaks or defects in the metal wire encased by the polyurethane. These are very tiny wires, he cannot remove the covering and he needs to look at the wire in sections for defects. We have a super SEM with a backscatter detector but no carbon coater. Any one have experience with this type of sample? How can I prep the samples for the SEM?? Any suggestions would be greatly appreciated!! Thanks! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
==============================Original Headers============================== 3, 21 -- From pekysar-at-ucdavis.edu Thu Jan 17 10:28:28 2008 3, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGSRFu017857 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:28:28 -0600 3, 21 -- Received: from mpat3317 ([169.237.197.34]) 3, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with SMTP id m0HGSQoB007412 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 08:28:27 -0800 (PST) 3, 21 -- Message-ID: {001701c85926$549f8970$22c5eda9-at-ucdsom.ucdavis.edu} 3, 21 -- From: "Pat Kysar" {pekysar-at-ucdavis.edu} 3, 21 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 21 --
I have been using a Hitachi 4800 SEM to image E. coli bacteria, but am not sure if what I've been seeing is representative of good fixation or sample preparation.
So far I've used a number of different fixatives (both with and without microwave assistance), the latest being 1% PFA, 1% GA, 50 mM cacodylate, pH 7.4. I have not been using any osmium. My main concern comes from the fact that the E. coli always look a bit bumpy and textured, not smooth and featureless like the B. subtilis I have also been imaging with more success. Also, I almost never see flagella.
My question is if anyone has recommendations for some resources in which I could find high resolution images of high-quality E. coli samples. When I try doing a Google Images search, or a quick look at PubMed, the micrographs I find are of considerably less resolution than I typically get with the Hitachi 4800 I've been using, so I'm never sure if the surface texture is normal and expected or completely anomalous.
Many thanks, Nate Chongsiriwatana
Graduate Student Northwestern University Department of Chemical and Biological Engineering
==============================Original Headers============================== 5, 20 -- From nathanielchongsiriwatana2008-at-u.northwestern.edu Fri Jan 18 16:06:13 2008 5, 20 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.177]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0IM6DdZ004719 5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2008 16:06:13 -0600 5, 20 -- Received: by py-out-1112.google.com with SMTP id p76so1642904pyb.6 5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2008 14:06:13 -0800 (PST) 5, 20 -- Received: by 10.110.39.5 with SMTP id m5mr2356250tim.55.1200693971970; 5, 20 -- Fri, 18 Jan 2008 14:06:11 -0800 (PST) 5, 20 -- Received: by 10.70.31.17 with HTTP; Fri, 18 Jan 2008 14:06:11 -0800 (PST) 5, 20 -- Message-ID: {2d96bb2a0801181406j256d4e1asc419ea1e6006dba6-at-mail.gmail.com} 5, 20 -- Date: Fri, 18 Jan 2008 16:06:11 -0600 5, 20 -- From: "Nathaniel Chongsiriwatana" {npchong-at-northwestern.edu} 5, 20 -- Sender: nathanielchongsiriwatana2008-at-u.northwestern.edu 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: SEM - resources for images of well-prepared E. coli? 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- Content-Disposition: inline 5, 20 -- X-Google-Sender-Auth: b4be85d1eb58bf35 ==============================End of - Headers==============================
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Email: vavv2009-at-yahoo.com Name: Arnold Villanueva
Question: I am wondering where one can get education (or some type of college degree or certification) to become a Microscopist .. for example, how does one obtain an associates degree (or certificate or Bachelor's) in Microscopy? any information would be greatly appreciated .. thank you!
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Email: pgan-at-ap.ansell.com Name: Gan Phay Fang
Organization: Ansell Shah Alam Sdn Bhd
Title-Subject: [Filtered] ISO 17025 Accreditation on SEM/EDX Analysis
Question: We are thinking of getting an ISO 17025 Accreditation on the SEM/EDX analysis. Our analysis is mostly getting the SEM micrographs and EDX analysis on the polymeric materials. Any advice and comments on starting up the preparation for the ISO 17025 Accreditation are much appreciated.
An Associates degree as an electron microscopy technician is available from the Madison Area Technical College (MATC) in Madison, WI, as well as from the San Joaquin Delta College of Stockton, CA. Central Michigan University in Mount Pleasant, MI also has an excellent program that combines an electron microscopy emphasis with an undergraduate biology degree. These are the programs that I am aware of.
Yours, Matthew Stephenson Impact Analytical/Michigan Molecular Institute
-----Original Message----- X-from: vavv2009-at-yahoo.com [mailto:vavv2009-at-yahoo.com] Sent: Monday, January 21, 2008 9:41 AM To: stephenson-at-impactanalytical.com
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please copy both vavv2009-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: vavv2009-at-yahoo.com Name: Arnold Villanueva
Question: I am wondering where one can get education (or some type of college degree or certification) to become a Microscopist .. for example, how does one obtain an associates degree (or certificate or Bachelor's) in Microscopy? any information would be greatly appreciated .. thank you!
Gan, is accreditation really needed? When our quality system was staring up and gelling (QS 9000, ISO 17025, now TS 16949), there was a lot of discussion in our company early on about how our SEM would fit in. The end result is that our SEM and EDS are not in our company's quality system. It is more of a diagnostic tool and it doesn't factor in to the production of our product (bearings). Our unit has a sticker on it that says "Not for product conformance".
Face it, The SEM is just an imaging device like a fancy camera. Are your cameras in the quality system?... probably not. Some people think because they have a highly expensive and technical piece of equipment, that it needs to be in the quality system. If you're measuring something, then this issue merits consideration.
As far as EDS analysis goes, I report semiquantitative results without numbers. My results are described in words, because numbers imply accuracy, and any quality auditor (or client) will want to know the error limits of reported numbers.
Stu Smalinskas, P.E. SKF North American Technical Center Plymouth, Michigan (734) 414-6862
--- pgan-at-ap.ansell.com wrote: } Email: pgan-at-ap.ansell.com } Name: Gan Phay Fang } } Organization: Ansell Shah Alam Sdn Bhd } } Title-Subject: [Filtered] ISO 17025 Accreditation on } SEM/EDX Analysis } } Question: We are thinking of getting an ISO 17025 } Accreditation on the SEM/EDX analysis. Our analysis } is mostly getting the SEM micrographs and EDX } analysis on the polymeric materials. Any advice and } comments on starting up the preparation for the ISO } 17025 Accreditation are much appreciated. } }
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==============================Original Headers============================== 7, 20 -- From smalinskas-at-yahoo.com Mon Jan 21 09:54:49 2008 7, 20 -- Received: from web34406.mail.mud.yahoo.com (web34406.mail.mud.yahoo.com [66.163.178.155]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0LFsm45023827 7, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Mon, 21 Jan 2008 09:54:48 -0600 7, 20 -- Received: (qmail 58235 invoked by uid 60001); 21 Jan 2008 15:54:48 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 20 -- b=iEnq4bAb1z8p8Sk8JYubgMOeyi+uZflUVWAg6+q/xTZr+dzf8O4+tlbv1u/2xly57GcPG/sZpCZ+uCXEoT48meUmi+c8Cw3D7e+7APMZN3vXAr2V7MWaeN75DuP+66cIQUHGbszTs8XG2wRHKhiUE4Eo3cBWmfT89TIrZ3znGcA=; 7, 20 -- X-YMail-OSG: Yj6FY.IVM1mqdKVW3.jO6.Gj1f3_mqyzPnprbOx_pMkB3.6VgG6Hd0cVTRjjFZ1PV_9Yps8LMcBxrWpebSjtr2xMpTCzrx0WOfh7kt_VUUlxUmkVg.A- 7, 20 -- Received: from [141.151.33.213] by web34406.mail.mud.yahoo.com via HTTP; Mon, 21 Jan 2008 07:54:47 PST 7, 20 -- Date: Mon, 21 Jan 2008 07:54:47 -0800 (PST) 7, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: ISO 17025 Accreditation on SEM/EDX Analysis 7, 20 -- To: pgan-at-ap.ansell.com, microscopy-at-ns.microscopy.com 7, 20 -- In-Reply-To: {200801211437.m0LEb8lF018849-at-ns.microscopy.com} 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=iso-8859-1 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Message-ID: {928897.43407.qm-at-web34406.mail.mud.yahoo.com} ==============================End of - Headers==============================
FEI servicemen recommend using only distilled or deionized (DI) water in chilled water recirculator systems.
A very effective algae killer is a long chain quaternary ammonium salt surfactant that you can buy at any quality swimming pool dealer. IMO, these quat's are much much better at killing algae than adding available chlorine from whatever chlorine source. The dosage rate for the very long chained surfactant is something like one quart per 10,000 gallon of water. That works out to a low PPM level of a biocide surfactant. Adding a chlorine-based chemical generates soluble chloride ions that can damage and can pit corrode stainless steel in DP tubing or SS fittings with O-ring channels.
There is another side to all this but this next side is rarely discussed. Algae problems are always discussed on the list server.
If you are running pure DI water, then how can your microscope ever plug up from green scale? On July 30, 2007 I emailed my FEI serviceman with 25-30 years of microscope experience with Philips-FEI and asked about algae and scaling. He emailed me the answers. No microscopes that they were asked to unplug were ever plugged up from algae. It was always green scale that caused the plugging and the scale was removed with a weak organic acid.
Here are the two sides of the chiller water problem in equation form.
This is the standard belief of the cause of green chiller water and is shown below as a simplified photosynthesis equation. Quat's efficiently interrupt the algae cycle on the left-hand side of the equation by breaking open algae cell walls and killing them. So they never really have a chance to multiply in a properly run system. Dissociated hypochlorous acid (HClO) from any chlorine source kills them too but generates additional chloride ions.
Notice that CO2 is involved in both reactions. Killing or preventing algae with a surfactant, an available chlorine oxidizer (hypochlorous acid), or stopping sunlight will eventually stop the first reaction. Stopping reaction one only shifts the chemistry focus to reaction two. The problem is that large stirred chiller systems dissolve more O2 and CO2 at higher rates than smaller chilled water systems, in my experience. These gases are dissolved in the pure DI water from the stirring and/or air exposure by the chiller's unsealed and open reservoir tank of water. Reaction two is slow and is normally unnoticed until the microscope plugs up. It takes awhile to notice the copper ion build up unless you regularly test the chilled water for PPM levels of copper by AA spectroscopy to determine the rate of copper ion buildup. Furthermore, short light paths of one to three centimeters or looking through a 6 mm piece of transparent tubing is just not that effective in spotting the first stages of soluble copper ion build up by trying to detect a faint initial green color.
I could say much more about the mechanisms, the effect of copper surface area of exposure, scale distribution in pipes, mechanical devices to add, and how the Ksp of copper carbonate enters into all this. Here are simple tests and hints to sort out the situation.
1. Test One. Adding a quaternary ammonium salt surfactant to green water will kill algae in about an hour. You can use excessive hypochlorite ion (bleach) on a chiller water sample also. A loss of green color means that a lot of algae were present. 2. Test Two. Adding ammonium hydroxide to a sample of green chiller water will turn any dissolved PPM levels of copper ions dark blue at a very high pH. A positive dark blue copper complex color means that soluble copper ions are being formed and those ions are tinting the water green. 3. BOTH of these two tests should be done to sort out what your system is doing. 4. PPM levels of copper ions will kill algae. 5. So you should either have a green algae problem or you have a green dissolved copper ion problem. When the copper ions build up in solution from the continuous dissolution of CO2 and O2, they will exceed the copper carbonate Ksp and start to precipitate the carbonate to form the green basic copper carbonate scale shown above. 6. In order to prevent scale formation, you MUST periodically drain out 25%-50% of the chiller water every 3-6 months. This draining and refilling action is another way to prevent the copper ions and carbonate ions from exceeding the Ksp value of copper carbonate, which has a lower Ksp than CaCO3. 7. If forced to use city water, you should flush out the "hard" city water in instrument dead volumes with DI water to help avoid any mixed carbonate scaling involving Ca and Mg. 8. Items 6 & 7 almost make an automatic DI water refill system at the reservoir tank mandatory in large systems. It only has to run when you will be flushing, refilling, or performing some other planned loss of chilled water.
Disclaimer: The way additional mechanical features and the chemistry interact can vary a lot in a custom installed central system and with the various needs of differnet user's instruments. It is important that users of central chilled water recirculators understand how their actions, such as flushing with city water, can have an impact on other users. IMO, this training is absolutely necessary for users and needed to make facility engineers understand why certain devices added to a central system are needed. This carbonate is the green corrosion product that slowly forms on copper roofs and gutters from only air and water exposure. The first thin brown scale color on copper roofs and in pipes is black to dark-brown copper oxide (CuO). After that forms and with further CO2 exposure, reaction two is followed.
HTH,
Paul Beauregard Senior Research Associate, retired Greensburg, PA 724-834-2247
==============================Original Headers============================== 17, 28 -- From beaurega-at-westol.com Mon Jan 21 11:22:28 2008 17, 28 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0LHMRe1009250 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 11:22:28 -0600 17, 28 -- X-Winbeam-MailScanner-Watermark: 1201540666.03028-at-rbmMn+OtGx2c+3wAVSvNqg 17, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 17, 28 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id m0LHHT8h024130 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 12:17:30 -0500 17, 28 -- Received: (qmail 17002 invoked by uid 89); 21 Jan 2008 17:17:29 -0000 17, 28 -- Received: from pitts-69-72-117-190.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.190) 17, 28 -- by mail.winbeam.com with SMTP; 21 Jan 2008 17:17:29 -0000 17, 28 -- Message-Id: {3.0.6.32.20080121121728.007b6560-at-pop3.norton.antivirus} 17, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified) 17, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 17, 28 -- Date: Mon, 21 Jan 2008 12:17:28 -0500 17, 28 -- To: microscopy-at-microscopy.com 17, 28 -- From: Beaurega {beaurega-at-westol.com} 17, 28 -- Subject: [Microscopy] RE: Product for watercooling 17, 28 -- Mime-Version: 1.0 17, 28 -- Content-Type: text/plain; charset="iso-8859-1" 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 17, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 17, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 17, 28 -- SpamAssassin (not cached, score=-5.139, required 4, 17, 28 -- autolearn=not spam, AWL 0.36, BAYES_00 -0.50, local_FROM_WB -1.00, 17, 28 -- local_HAM_FROM_WB -4.00) 17, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
I only have experience in sectioning seeds of Orobanche cumana. I was successful doing semi- and ultrathin sections after embedding in LR-White. The first prepartion step was to perforate the seeds with a fine needle. Without perforating the seed coat, it was impossible to get anything into these tiny seeds.
I think seeds are always diffcult, because their coat is quite tight, their water content extremely low, and they store masses of starch grains or other storage material which are difficult to fix and to section. Watermelon seeds are quite large. Maybe you can cut them and try to embed the pieces?
Good Luck, Anne
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } Happy New Year! } Does anyone have experience thick-sectioning watermelon seeds? We're } working with mature seeds - black seed coat - unfixed, not embedded. } The seeds are left in a moist chamber overnight to imbibe water. } Vibratome sectioning didn't work well. Any suggestions? Would fixing } and embedding make them easier to section? } } I think they're really only good for spitting...but the researcher } doesn't want to hear that;-) } Any help would be greatly appreciated. } Many thanks, } Beth } } Beth Richardson } Plant Biology Department } University of Georgia } Athens, GA 30602 } 706-542-1790 } } } ==============================Original Headers============================== } 4, 19 -- From beth-at-plantbio.uga.edu Thu Jan 17 10:21:22 2008 } 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu } [128.192.26.2]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m0HGLLmX009715 } 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:21:21 -0600 } 4, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) } 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) } 4, 19 -- by dogwood.plantbio.uga.edu } 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) } 4, 19 -- for microscopy-at-microscopy.com; } 4, 19 -- Thu, 17 Jan 2008 11:21:15 -0500 } 4, 19 -- Message-Id: {80AFBBC9-F649-4422-84BA-331CE918C9EB-at-plantbio.uga.edu} } 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes } 4, 19 -- Content-Transfer-Encoding: 7bit } 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) } 4, 19 -- Subject: sectioning watermelon seeds } 4, 19 -- Date: Thu, 17 Jan 2008 11:22:05 -0500 } 4, 19 -- X-Mailer: Apple Mail (2.915) } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 32 -- From heller-at-uni-hohenheim.de Mon Jan 21 14:44:01 2008 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0LKi0pI030518 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 14:44:01 -0600 7, 32 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 806DC11D401 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:58 +0100 (CET) 7, 32 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 7, 32 -- by localhost (webmail3.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 7, 32 -- with ESMTP id UrTnPOubZH1G for {Microscopy-at-microscopy.com} ; 7, 32 -- Mon, 21 Jan 2008 21:43:52 +0100 (CET) 7, 32 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 4E35B11D3FB 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 7, 32 -- id CA7B060153; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 7, 32 -- by webmail.uni-hohenheim.de (IMP) with HTTP 7, 32 -- for {heller-at-10.0.0.5} ; Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- Message-ID: {1200948231.47950407b2ce8-at-webmail.uni-hohenheim.de} 7, 32 -- Date: Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- From: heller-at-uni-hohenheim.de 7, 32 -- To: Microscopy-at-microscopy.com 7, 32 -- Subject: sectioning watermelon seeds 7, 32 -- References: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Type: text/plain; charset=ISO-8859-1 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 7, 32 -- X-Originating-IP: 144.41.103.45 ==============================End of - Headers==============================
I am not specialist in botanics, but my common sense tells me that perhaps you could cut the seed in 2 in the length, it would (1) make it thin enough for the fixative to efficiently penetrate (2) avoid the need to perforate the coat. If it is still too thick (for example the tissue next to the coat has artifacts), you could perhaps cut slices.
Regards,
Stephane
----- Original Message ---- X-from: "heller-at-uni-hohenheim.de" {heller-at-uni-hohenheim.de} To: nizets2-at-yahoo.com Sent: Monday, January 21, 2008 9:49:20 PM
Dear Beth,
I only have experience in sectioning seeds of Orobanche cumana. I was successful doing semi- and ultrathin sections after embedding in LR-White. The first prepartion step was to perforate the seeds with a fine needle. Without perforating the seed coat, it was impossible to get anything into these tiny seeds.
I think seeds are always diffcult, because their coat is quite tight, their water content extremely low, and they store masses of starch grains or other storage material which are difficult to fix and to section. Watermelon seeds are quite large. Maybe you can cut them and try to embed the pieces?
Good Luck, Anne
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } Happy New Year! } Does anyone have experience thick-sectioning watermelon seeds? We're } working with mature seeds - black seed coat - unfixed, not embedded. } The seeds are left in a moist chamber overnight to imbibe water. } Vibratome sectioning didn't work well. Any suggestions? Would fixing } and embedding make them easier to section? } } I think they're really only good for spitting...but the researcher } doesn't want to hear that;-) } Any help would be greatly appreciated. } Many thanks, } Beth } } Beth Richardson } Plant Biology Department } University of Georgia } Athens, GA 30602 } 706-542-1790 } } } ==============================Original Headers============================== } 4, 19 -- From beth-at-plantbio.uga.edu Thu Jan 17 10:21:22 2008 } 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu } [128.192.26.2]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m0HGLLmX009715 } 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:21:21 -0600 } 4, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) } 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) } 4, 19 -- by dogwood.plantbio.uga.edu } 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) } 4, 19 -- for microscopy-at-microscopy.com; } 4, 19 -- Thu, 17 Jan 2008 11:21:15 -0500 } 4, 19 -- Message-Id: {80AFBBC9-F649-4422-84BA-331CE918C9EB-at-plantbio.uga.edu} } 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes } 4, 19 -- Content-Transfer-Encoding: 7bit } 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) } 4, 19 -- Subject: sectioning watermelon seeds } 4, 19 -- Date: Thu, 17 Jan 2008 11:22:05 -0500 } 4, 19 -- X-Mailer: Apple Mail (2.915) } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 32 -- From heller-at-uni-hohenheim.de Mon Jan 21 14:44:01 2008 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0LKi0pI030518 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 14:44:01 -0600 7, 32 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 806DC11D401 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:58 +0100 (CET) 7, 32 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 7, 32 -- by localhost (webmail3.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 7, 32 -- with ESMTP id UrTnPOubZH1G for {Microscopy-at-microscopy.com} ; 7, 32 -- Mon, 21 Jan 2008 21:43:52 +0100 (CET) 7, 32 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 4E35B11D3FB 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 7, 32 -- id CA7B060153; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 7, 32 -- by webmail.uni-hohenheim.de (IMP) with HTTP 7, 32 -- for {heller-at-10.0.0.5} ; Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- Message-ID: {1200948231.47950407b2ce8-at-webmail.uni-hohenheim.de} 7, 32 -- Date: Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- From: heller-at-uni-hohenheim.de 7, 32 -- To: Microscopy-at-microscopy.com 7, 32 -- Subject: sectioning watermelon seeds 7, 32 -- References: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Type: text/plain; charset=ISO-8859-1 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 7, 32 -- X-Originating-IP: 144.41.103.45 ==============================End of - Headers==============================
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==============================Original Headers============================== 20, 20 -- From nizets2-at-yahoo.com Tue Jan 22 06:29:57 2008 20, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0MCTv0R011560 20, 20 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jan 2008 06:29:57 -0600 20, 20 -- Received: (qmail 86931 invoked by uid 60001); 22 Jan 2008 12:29:57 -0000 20, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 20 -- s=s1024; d=yahoo.com; 20, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 20, 20 -- b=6oC041OI8SU/KOraTWvfimPYAwKv35IFKryfR1ute6M6+i42b9g/GMbJNgWRGLL2gzJ9t1TLRm4r7jjCAEYFQPDiynzhu7PSXEFHqRFrJcbiBnPy9wrDRRUcGCP4IgQUtnd6mSWczx968yt0lEufGukRpNTMlUdvfMCRZ+Fiobo=; 20, 20 -- X-YMail-OSG: 2_q8r1IVM1nQHcRer8i4DDUeJEFsvREdXU5uUCLixlw70swFy0fXkLginyQ_AEhoEfU034wft0tOn8DZKR4KnfTfRHqcHKtRhpexRhLbnMo6z5r9Sd6oWlTdW08CQTc73VtNd0jmL.ChOBM5odO_sz83l1MuH4aoqlsz3WdiqVHn 20, 20 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Tue, 22 Jan 2008 04:29:56 PST 20, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.160 20, 20 -- Date: Tue, 22 Jan 2008 04:29:56 -0800 (PST) 20, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 20, 20 -- Subject: Re: [Microscopy] sectioning watermelon seeds 20, 20 -- To: heller-at-uni-hohenheim.de 20, 20 -- Cc: microscopy-at-microscopy.com 20, 20 -- MIME-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=us-ascii 20, 20 -- Message-ID: {195330.86606.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Email: susan.trant-at-viha.ca Name: Susan Trant
Organization: Vancouver Island Health Authority
Title-Subject: [Filtered] Jeol 100CX-II
Question: Hello Everyone
We have purchased a new Jeol TEM microscope. We are asking anyone out there if they would like our old Jeol 100CX-II. The vendors have told us that they will crate the microscope up and then it is ours to dispose of. The lucky party must pay to have the microscope shipped to their location.
Sue Trant EM Technologist Vancouver Island Health Authority 1952 Bay Street Victoria BC V8R 1J8 250-370-8402
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Title-Subject: [Filtered] Project MICRO: New Sandbox Contact
Question: We are pleased to announce that the Microscopy Society of Americaís (MSA) collection of sand for use with Project MICRO Microscopic Explorations activity 6 is now housed at McCrone Associates, Inc. in Westmont , Illinois; and is under the direction of Heidi Ullberg. Mr. Joe Neilly has faithfully dispatched sand samples to educators all over the country for many years. Joe has enjoyed his duties as ëKeeper of the Sandboxí, and as he passes the shovel to Heidi says that he will miss visiting the far reaches of the world - one sand sample at a time.
To view an inventory of the collection visit MSAís website at:
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Email: holsen-at-awscorp.com Name: Harald Olsen
Organization: American World Services
Title-Subject: [Filtered] vacuum pump applications to SEM
Question: Hello,
I work at American World Services in Washington, D.C., we represent the French vacuum pump company Normetex. I was referred to this listserv by Jon Norenburg of the American Microscopical Society as a way to get an answer to a question I have concerning and application for a specific type of ultrahigh vacuum pump.
I have heard that SEM and TEM rely on vacuum pumps, I am trying to figure out if they are similar to the product we represent. Normetex's pumps have traditionally been used in nuclear applications, as they are perfectly clean and dry and thus suited to a clean room environment. Because of their ability to safely handle corrosive or inert gases, I was wondering if they might also have an application with electron microscopy.
In terms of specifications, Normetex produces pumps that range in size from 15 m3/h to 600 m3/h, and produce an ultimate vacuum of 45 mbar on smaller models and 8x10-2 mbar on the larger models.
I am trying to figure out if these pumps would be appropriate for SEM or TEM, as well as who might best be able to make use of them. I realize that this is a very specific question, but I trust that if any resource would have an answer, it would be the members of this listserv.
Thank you,
Harald Olsen Project Assistant American World Services Corp. 1247 Wisconsin Ave., NW, Suite 201 Washington, DC 20007 (t) +1.202.296.3523 (f) +1.202.333.0017 holsen-at-awscorp.com www.awscorp.com
Has anyone on the list had any experiences (Good or Bad) updating the refrigeration system on a water chiller from R-12 to a current refrigerant (i.e. R-134a, etc.)?
We have had a older R-12 Haskris chiller die, and are looking at the possibility of replacing the compressor and condenser (water cooled), blowing and flushing out the lines, and refilling with a current R-12 replacement. The question is the mineral oil in the system. The R- 12 will replace easily, but what about the residual mineral oil.
Five years ago we updated a chiller, replacing the condenser and compressor, but we used synthetic oil (instead of mineral oil) however we still had R-12 so that´s what we used. The system has run perfectly fine 24/7 for 5 years. This would seem to say oil incompatibility is not a significant issue.
O.k., I´m being cheap. If I had $6000 I´d buy a new water chiller and be done with it. But I don´t. I´m hoping for a lower cost solution that will get me some time. Any thoughts?
Yes, we have professional refrigeration folks working on it but they are not sure on the smaller systems.
(Disclaimer: I have no financial interests in any refrigeration company, and have been working with Haskris systems for 25 years and love them.)
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 12, 23 -- From edelmare-at-muohio.edu Wed Jan 23 07:23:00 2008 12, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0NDMxco018731 12, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 23 Jan 2008 07:23:00 -0600 12, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 12, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m0NDMxZT009539 12, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 23 Jan 2008 08:22:59 -0500 12, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 12, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m0NDMx0D029237 12, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 23 Jan 2008 08:22:59 -0500 12, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 23 -- To: microscopy-at-Microscopy.com 12, 23 -- Date: Wed, 23 Jan 2008 08:22:59 -0500 12, 23 -- MIME-Version: 1.0 12, 23 -- Subject: Refurbishing - Updating Water chillers 12, 23 -- Message-ID: {4796F963.15055.D82BBEC-at-edelmare.muohio.edu} 12, 23 -- Priority: normal 12, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 23 -- Content-type: text/plain; charset=ISO-8859-1 12, 23 -- Content-description: Mail message body 12, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id m0NDMxco018731 ==============================End of - Headers==============================
Our highschool has a Cambridge S200 SEM that was donated to us by Motorola. They actually donated two, and after months of assembling the most trustworthy pieces together as one unit, the thing now powers up, the pumps work and the screen lights up. All I can see on the screen is snow. No moving of apertures, stage or console controls seem to affect what is seen on the screen or produces an image. I am reasonably sure that the Wallace unit is putting out all the high voltages, so the detector should be getting power. The detector was removed from the scope and tested with a light, and it does produce a signal under these circumstances. Connected back to the column though, I see no signal on my oscilloscope at the test point on the input board for the signal from the detector, suggesting it is not doing anything. Feeding a test signal into the microscope at this same test point, I can visualize the signal on the screen, so the video electronics are working.
The filament fail light goes out when I turn up the filament, so I assume electrons should be speedng down the column and hitting the specimen. To the best of my ability the filament is centered and aligned in the cap properly.
This seems to be the only remaining issue with the microscope, after solving numerous others, but it has me stumped. The detector apparantly works, but it isn't working! Any advice?
-Dr. Mike Brown Science Dept Chair AAEC_PV
==============================Original Headers============================== 8, 17 -- From mbrown-at-aaechighschools.com Wed Jan 23 13:05:04 2008 8, 17 -- Received: from smtpoutwbe08.prod.mesa1.secureserver.net (smtpoutwbe08.prod.mesa1.secureserver.net [208.109.78.210]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0NJ54jp025976 8, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Jan 2008 13:05:04 -0600 8, 17 -- Received: (qmail 26874 invoked from network); 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: from unknown (HELO gem-wbe24.prod.mesa1.secureserver.net) (64.202.189.227) 8, 17 -- by smtpoutwbe08.prod.mesa1.secureserver.net with SMTP; 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: (qmail 15048 invoked by uid 99); 23 Jan 2008 19:05:03 -0000 8, 17 -- Date: Wed, 23 Jan 2008 12:05:03 -0700 8, 17 -- From: mbrown-at-aaechighschools.com 8, 17 -- Subject: Issues with a Cambridge S200 SEM 8, 17 -- To: Microscopy-at-Microscopy.Com 8, 17 -- Message-ID: {20080123120503.889a5134facffa13190a02200f773d01.cdade38cdc.wbe-at-email.secureserver.net} 8, 17 -- MIME-Version: 1.0 8, 17 -- Content-Type: TEXT/plain; CHARSET=US-ASCII 8, 17 -- User-Agent: Web-Based Email 4.12.20 8, 17 -- X-Originating-IP: 71.35.53.132 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa
Title-Subject: [Filtered] Allied Epoxy Bond 110
Question: I recently found Allied's 2-part Epoxy Bond 110 in my lab but could not locate the mixing literature. I've emailed Allied but am rather impatient...Does anyone know the ratio of resin to hardener and the temperature necessary to cure the epoxy?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both swaffordisjim-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: swaffordisjim-at-gmail.com Name: Jim Swafford
Organization: retired on Dinjim ranch
Title-Subject: [Filtered] MT1 Ultra microtome
Question: Greetings. I am wanting to purchase a particular model of ultra microtome, Sorvall MT1, which was very popular in the late 1950's through 1960's. I anticipate using this instrument at Pittsburg State University which is a small school, ~6,000 students, located in Southeastern Kansas. If anyone knows the location of one of these microtomes that could be sold or donated, please contact me.
For quite some time I have wondered why NUNC did not make a Permanox Petri Dish smaller than 60 x 15 mm. I have grown to really appreciate the advantages of using the Permanox over standard Polystyrene dishes for tissue cultured cells grown for TEM experiments since every cell line that I have tried adheres well to them, they can withstand chemicals like acetone and propylene oxide that dissolve the polystyrene and the embedded cells come away from the Permanox so easily and smoothly.
With the special cells and reagents that I am now using for TEM, it is a big waste to grow cells over such a large area when a 35 x 15 mm dish would do nicely. I do want a dish not a chambered slide.
I would like to know if there are others (you) 1. who would switch to a smaller dish if they would be made available. (this is my pick) 2. who would like to use both the 60mm and 35mm dishes 3. who would only use the 60mm dishes If I get a reasonable response I will contact the company with my results to back up my request that they consider making the smaller dishes.
Comments are welcome.
For the survey, it may be best to answer me "Off-ListServer" so as not to fill up the emails of other members. I will let the ListServer know the tally after the replies come in.
Thanks to all, Pat
Patricia Stranen Connelly Biologist, Electron Microscopy NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov
==============================Original Headers============================== 10, 22 -- From connellyps-at-nhlbi.nih.gov Wed Jan 23 17:57:23 2008 10, 22 -- Received: from NIHCESSMTP3.hub.nih.gov (nihcessmtp3.hub.nih.gov [128.231.90.117]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0NNvNkj008142 10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 23 Jan 2008 17:57:23 -0600 10, 22 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 10, 22 -- Wed, 23 Jan 2008 18:57:18 -0500 10, 22 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.167]) with Microsoft Exchange Server HTTP-DAV ; 10, 22 -- Wed, 23 Jan 2008 23:57:18 +0000 10, 22 -- User-Agent: Microsoft-Entourage/11.3.6.070618 10, 22 -- Date: Wed, 23 Jan 2008 18:54:47 -0500 10, 22 -- Subject: Interest in smaller "Permanox" dishes? 10, 22 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 22 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 22 -- Message-ID: {C3BD3DF7.13F0%connellyps-at-nhlbi.nih.gov} 10, 22 -- Thread-Topic: Interest in smaller "Permanox" dishes? 10, 22 -- Thread-Index: AcheG1Vmk7YdFMoOEdyXCwANk2Yv1A== 10, 22 -- Mime-version: 1.0 10, 22 -- Content-type: text/plain; 10, 22 -- charset="ISO-8859-1" 10, 22 -- X-OriginalArrivalTime: 23 Jan 2008 23:57:18.0661 (UTC) FILETIME=[AFCC9F50:01C85E1B] 10, 22 -- Content-Transfer-Encoding: 8bit 10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0NNvNkj008142 ==============================End of - Headers==============================
I have a user who was trying to use a camera without microscope to image something that he could not get on the stage of the microscope. In playing around, he discovered that Nikon objectives actually have a C-mount compatible thread. When he screwed a 2cm extender and then a 20x objective onto the CCD camera, he was able to image a specimen at a distance of about 2cm. Naively, I would have said that an infinity corrected lens should not form an image in the plane of the CCD chip, but obviously, the specimen is not at the appropriate focal point of the lens (~2mm). Can someone explain why this works? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 4, 19 -- From david.knecht-at-uconn.edu Thu Jan 24 08:19:42 2008 4, 19 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0OEJgCb025204 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 24 Jan 2008 08:19:42 -0600 4, 19 -- Received: from d46h174.public.uconn.edu (d46h174.public.uconn.edu [137.99.46.174]) 4, 19 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id m0OEJY9H005844 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 24 Jan 2008 09:19:34 -0500 4, 19 -- Message-Id: {D27EFCEF-F4C3-469F-9367-6E949899AAB5-at-uconn.edu} 4, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) 4, 19 -- Subject: Infinity corrected objectives 4, 19 -- Date: Thu, 24 Jan 2008 09:19:34 -0500 4, 19 -- X-Mailer: Apple Mail (2.915) 4, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 4, 19 -- X-UConn-MailScanner: Found to be clean 4, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
Mike, Are you getting emission current? Does it (emission current) appear to behave properly as you heat the filament? If not, then there is some kind of problem with the gun circuit. If so, I'd say the beam isn't making it down the column. Are the various beam-steering circuits behaving properly? Have you tried pulling out all the apertures to try and get some response? Are both the upper and lower scan coils functioning properly? Any of the coils have the potential to drive the beam off to the side somewhere and make it disappear.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: mbrown-at-aaechighschools.com [mailto:mbrown-at-aaechighschools.com] Sent: Wednesday, January 23, 2008 2:08 PM To: kenconverse-at-qualityimages.biz
Our highschool has a Cambridge S200 SEM that was donated to us by Motorola. They actually donated two, and after months of assembling the most trustworthy pieces together as one unit, the thing now powers up, the pumps work and the screen lights up. All I can see on the screen is snow. No moving of apertures, stage or console controls seem to affect what is seen on the screen or produces an image. I am reasonably sure that the Wallace unit is putting out all the high voltages, so the detector should be getting power. The detector was removed from the scope and tested with a light, and it does produce a signal under these circumstances. Connected back to the column though, I see no signal on my oscilloscope at the test point on the input board for the signal from the detector, suggesting it is not doing anything. Feeding a test signal into the microscope at this same test point, I can visualize the signal on the screen, so the video electronics are working.
The filament fail light goes out when I turn up the filament, so I assume electrons should be speedng down the column and hitting the specimen. To the best of my ability the filament is centered and aligned in the cap properly.
This seems to be the only remaining issue with the microscope, after solving numerous others, but it has me stumped. The detector apparantly works, but it isn't working! Any advice?
-Dr. Mike Brown Science Dept Chair AAEC_PV
==============================Original Headers============================== 8, 17 -- From mbrown-at-aaechighschools.com Wed Jan 23 13:05:04 2008 8, 17 -- Received: from smtpoutwbe08.prod.mesa1.secureserver.net (smtpoutwbe08.prod.mesa1.secureserver.net [208.109.78.210]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0NJ54jp025976 8, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Jan 2008 13:05:04 -0600 8, 17 -- Received: (qmail 26874 invoked from network); 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: from unknown (HELO gem-wbe24.prod.mesa1.secureserver.net) (64.202.189.227) 8, 17 -- by smtpoutwbe08.prod.mesa1.secureserver.net with SMTP; 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: (qmail 15048 invoked by uid 99); 23 Jan 2008 19:05:03 -0000 8, 17 -- Date: Wed, 23 Jan 2008 12:05:03 -0700 8, 17 -- From: mbrown-at-aaechighschools.com 8, 17 -- Subject: Issues with a Cambridge S200 SEM 8, 17 -- To: Microscopy-at-Microscopy.Com 8, 17 -- Message-ID: {20080123120503.889a5134facffa13190a02200f773d01.cdade38cdc.wbe-at-email.secure server.net} 8, 17 -- MIME-Version: 1.0 8, 17 -- Content-Type: TEXT/plain; CHARSET=US-ASCII 8, 17 -- User-Agent: Web-Based Email 4.12.20 8, 17 -- X-Originating-IP: 71.35.53.132 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 27 -- From kenconverse-at-qualityimages.biz Thu Jan 24 10:29:15 2008 23, 27 -- Received: from dpmailmta05.doteasy.com (dpmailmta05-20.doteasy.com [65.61.218.100]) 23, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0OGTFIq010206 23, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 24 Jan 2008 10:29:15 -0600 23, 27 -- Received: from dpmail16.doteasy.com (unverified [192.168.101.16]) 23, 27 -- by dpmailmta05.doteasy.com (DEO) with ESMTP id 24373207-1814644 23, 27 -- for multiple; Thu, 24 Jan 2008 08:30:05 -0800 23, 27 -- Received: from cpe-72-227-100-25.maine.res.rr.com [72.227.100.25] by dpmail16.doteasy.com with SMTP; 23, 27 -- Thu, 24 Jan 2008 08:28:56 -0800 23, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 23, 27 -- To: {mbrown-at-aaechighschools.com} , 23, 27 -- "MSA Listserver" {Microscopy-at-msa.microscopy.com} 23, 27 -- Subject: RE: [Microscopy] Issues with a Cambridge S200 SEM 23, 27 -- Date: Thu, 24 Jan 2008 11:28:46 -0500 23, 27 -- Message-ID: {000e01c85ea6$326e9870$6401a8c0-at-Ken} 23, 27 -- MIME-Version: 1.0 23, 27 -- Content-Type: text/plain; 23, 27 -- charset="us-ascii" 23, 27 -- X-Priority: 3 (Normal) 23, 27 -- X-MSMail-Priority: Normal 23, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 23, 27 -- Thread-Index: Achd81aEYK6eZdxhSSmTrIW/5AYgLAAsa/Ig 23, 27 -- In-Reply-To: {200801231908.m0NJ8Qmw030307-at-ns.microscopy.com} 23, 27 -- Importance: Normal 23, 27 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 23, 27 -- Content-Transfer-Encoding: 8bit 23, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0OGTFIq010206 ==============================End of - Headers==============================
Hi All - I would appreciate hearing from users of environmental SEMs, specifically as to imaging live cells. This is a new area for me, as my expertise is in TEM, so I am trying to gather as much information as possible.
We already have a variable pressure SEM, which is not ideal for viewing cells, although to my knowledge we have never tried it. I have been looking at manufacturers of ESEMs, and have found the Zeiss EVO LS and the FEI Quanta. Any user opinions on these two systems are welcome as well.
Thanks, Jessica ____________________ Jessica Cervantes Bend Research Inc Bend, OR 97701 www.bendres.com
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==============================Original Headers============================== 6, 14 -- From cervantes-at-bendres.com Thu Jan 24 10:53:47 2008 6, 14 -- Received: from mail.bendres.com (mail.bendres.com [216.228.161.112] (may be forged)) 6, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0OGrl0m023702 6, 14 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Jan 2008 10:53:47 -0600 6, 14 -- MIME-Version: 1.0 6, 14 -- Content-Type: text/plain; 6, 14 -- charset="iso-8859-1" 6, 14 -- Subject: ESEM Imaging of Live Cells 6, 14 -- Date: Thu, 24 Jan 2008 08:53:47 -0800 6, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F150887EEE5E9-at-BRIEX04A} 6, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 6, 14 -- To: {Microscopy-at-microscopy.com} 6, 14 -- Content-Transfer-Encoding: 8bit 6, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0OGrl0m023702 ==============================End of - Headers==============================
The answer is quite simple. For the sake of simplicity, a compound microscope has two key "imaging" lenses: the objective and the eyepiece. Optically, the job of the eyepiece is to create a (usually) magnified image at the appropriate location to act as a "specimen" for the eyepiece. In order for there to be an image, the light carrying the original specimen information must convege to a point of focus. There are two ways to achieve that result: a. To place the object just beyond the front focal plane of the objective (FFPo), resulting in a real image at some fixed distance b. To place the object exactly at the FFPo, sending the imaging information up through the optical train in a bundle of rays which is either parallel to the optic axis (on-axis info) or some principle ray at some angle to that axis (off-axis info). Note that if these rays are parallel, they cannot converge to form an image. We say that the information "goes to inifinity", hence never forms an image. In this case, you need a second lens (the telan or tube lense) to create the necessary convergence at the right location for the eyepiece. The space between the back focal plane of the objective and the tube lens is what is known as infinity space, a design which gives considerable freedom to microscope designers.
So, if you take a lens that was meant to work in Condition b and move the object slightly further away from the front of the lens, you will change the optics to Condition a. This is another one of those cases, like NA, where what is written on the objective is only true when the microscope is properly set up and aligned for Koehler illumination.
Hope this was helpful!
Best regards, Barbara Foster, President
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Thank you to the dozens of you who sent in suggestions. Most suggestions were that electrons were not making it down the column to the specimen. I removed one of the apertures. Electrons are now getting down the column to the specimen when I dial in the place where the aperture used to be. When I turn up the filament until the fail light goes off, the screen flashes, and the nature of the snow on the screen changes. I can visualize vertical stripes down the screen. These move around to the left and right when I move the specimen. Rotating the image moves the lines also, but they always stay vertical. In fact rotating the specimen also moves the vertical lines but does not make them horizontal.
What is the issue here?
-Dr. Mike Brown Science Dept Chair AAEC_PV
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This Question was submitted to Ask-A-Microscopist by (stephen.ruiz-at-siemens.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 24, 2008 at 14:41:03 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both stephen.ruiz-at-siemens.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: stephen.ruiz-at-siemens.com Name: Stephen Ruiz
Organization: Siemens DX
Education: Graduate College
Location: Norwood, Ma., USA
Title: Digital SLR Camera
Question: Any suggestions on a good SLR digital camera for both micro and macro images?
David sent me a follow-up to this posting, asking how to incorporate all of this info into teaching microscopy. I know that a number of you teach and might find the infomration useful, so here's a copy of my answer to him.
Dear David,
There is a really neat way to do all of this. It starts with a simple experiment with a hand lens. a. Difference between object and image Using a simple hand lens, have the students look through the lens at their finger nails (be prepared for lots of silly groans!). First have them put their finger close to the lens, then have them slowly move it back. As they do, the image of their finger will become larger and larger. At some point it will disappear. Then, if they keep watching carefully, the image will reappear, inverted. Then, have them remove the lens and look directly at their finger. At this point I tell the, "Notice that at no point did your finger leave your hand." This really solidifies the concept of object (their finger) and image (what they saw through the lens, after the lens has operated on that information. Take home message: our job as microscopists is to capture in the image, with as much fidelity as possible, the information from the object. Second take home messages: Lenses can lie.
b. Find the focal length of the lens Using a simple, single hand lens, have the students find the focus the image of the overhead lights on the table in front of them. The distance from the physical center of the lens to the table top is the focal length. This set up the following concepts: Focal length Focal plane Front focal plane
c. Four cases of lens Now that they understand the concept of object/image and focal length, you can repeat Experiment A to illustrate the case of the object (1) inside the focal length (forms virtual, upright image on same side of the lens as the object (2) at the focal length (informrtion goes to infinity: no convergence of date; No image) (3) slightly beyond the focal length (real image, on other side of the lens; magnification determined by distance of object from lens) (4) a great distance beyond the focal length (light coming from "infinity"; rays form bundle which is parallel to either optic axis or principle ray through optical axis). You can reinforce all of these using simple ray diagrams found in any high school physics book.
d. All of this sets up the discussion for (1) Infinity vs. fixed tube length optics and why you just can't willy nilly change objectives from stands of one design to stands of the other (2) Spherical and chromatic aberration (3) Which then leads to discussions of different types of glassware on the microscope and how to make educated buying decisions based on corrections, working distances.
It's a great set of lecture/demonstrations that really carries through to discussions of NA, resolution versus detection, and contrast techniques...a little bit of physics that goes a long way. And because they are doing demonstrations throughout the lecture, they stay involved AND tend to remember it all (every teachers' dream).
At this point, I'd recommend getting a copy of my book... it's all in there... but we have just come to the end of the supply. I need to talk to Zeiss, to see if they are interested in updating and reprinting. My other challenge is finding the time to do that. I do have some lecture notes that I use when I teach, but they are primarily just the diagrams, etc.
Hope this was helpful.
Best regards, Barbara
At 04:11 PM 1/24/2008, you wrote: } THanks for the explanation. It makes sense and the object(ive) lesson is especially important (abberations aside). I will have to think how I can incorporate this into my microscopy course. Dave } } On Jan 24, 2008, at 2:17 PM, {mailto:bfostermme-at-sbcglobal.net} bfostermme-at-sbcglobal.net wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Dear Group - what is your opinion for a TEM bottom mounted camera for the best resolution for high mag - camera with lens or fiber optic? Appreciate any comments. Thanks Barbara
==============================Original Headers============================== 1, 18 -- From maloneyb-at-fiu.edu Fri Jan 25 05:14:12 2008 1, 18 -- Received: from fmailhost06.isp.att.net (fmailhost06.isp.att.net [204.127.217.106]) 1, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0PBECAj022401 1, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 05:14:12 -0600 1, 18 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 18 -- by isp.att.net (frfwmhc06) with ESMTP 1, 18 -- id {20080125111411H0600colrte} ; Fri, 25 Jan 2008 11:14:11 +0000 1, 18 -- X-Originating-IP: [74.166.189.137] 1, 18 -- Message-ID: {4799C431.5010803-at-fiu.edu} 1, 18 -- Date: Fri, 25 Jan 2008 06:12:49 -0500 1, 18 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 1, 18 -- X-Accept-Language: en-us, en 1, 18 -- MIME-Version: 1.0 1, 18 -- To: Microscopy-at-microscopy.com 1, 18 -- Subject: digital camera with lens vs. fiber optic coupled camera 1, 18 -- Content-Type: text/plain; charset=us-ascii; format=flowed 1, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
only fiber optic coupling - as far as I can tell. best regards Reinhard Rachel --
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 5, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jan 25 07:23:23 2008 5, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0PDNMpS006661 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 25 Jan 2008 07:23:22 -0600 5, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with SMTP id C6CE5552FB 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 25 Jan 2008 14:23:26 +0100 (CET) 5, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 5, 25 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id C0F58552E8 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 25 Jan 2008 14:23:26 +0100 (CET) 5, 25 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 5, 25 -- with Novell_GroupWise; Fri, 25 Jan 2008 14:23:21 +0100 5, 25 -- Message-Id: {4799F0D4.044C.0054.0-at-biologie.uni-regensburg.de} 5, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 5, 25 -- Date: Fri, 25 Jan 2008 14:23:16 +0100 5, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 5, 25 -- To: {Microscopy-at-Microscopy.Com} 5, 25 -- Subject: [Microscopy] digital camera 5, 25 -- References: {200801251114.m0PBEYRH022815-at-ns.microscopy.com} 5, 25 -- In-Reply-To: {200801251114.m0PBEYRH022815-at-ns.microscopy.com} 5, 25 -- Mime-Version: 1.0 5, 25 -- Content-Type: text/plain; charset=US-ASCII 5, 25 -- Content-Disposition: inline 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0PDNMpS006661 ==============================End of - Headers==============================
Sales and Sales Support Positions Available in the USA
Position #1 – Central and South Eastern Region Sales Representative US / Canada
Bitplane is looking for a biologist with strong computer skills and at least one year of hands-on experience using a confocal or similar 3D advanced light microscope.
Feel free to forward this e-mail if know someone in your facility who would be interested.
The duties of this position include:
• Customer visits and analysis of customer's imaging needs. • Demonstration of the software and onsite work with the customer • Organization of exhibitions and workshops. • Sales support of existing customers. The candidate is expected to have an outgoing personality with strong communication skills and should look forward to increased responsibility. At least 50% travel will be required. Representative is required to live in the territory they cover. Benefits include a base salary, performance based commission, 401K plan, healthcare, and vacation. Representative will work out of a home office. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow. We don't mind if the candidate does not have much business experience and we are prepared to show him/her the sales skills at the job.
Position #2 – Sales Support Manager
Bitplane is looking for a candidate with a science background and knowledge of the confocal and 3D microscope community.
Feel free to forward this e-mail if know someone in your facility who would be interested.
The duties of this position include:
• Identifying potential new regional customers via web searches, literature searches, marketing campaigns, trade shows, and customer referrals. • Introduction of Bitplane products and services to potential customers via email, phone, and Webex. • Understanding potential customers needs related to products offered by Bitplane. • Organizing and planning workshops and demonstrations for regional sales representatives
The candidate is expected to have an outgoing personality with strong communication skills and should look forward to increased responsibility. Travel not required. Benefits include a base salary, 401K plan, healthcare, and vacation. Representative will work out of a home office. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow.
Bitplane is an international company specializing in the sale of software for the visualization and analysis of 3D and 4D microscope images. More information can be found at www.bitplane.com
Interested persons should respond directly to Michael C. Wussow (mike-at-bitplane.com 651-336-4600) indicating which position they are interested in and providing a copy of their CV.
Bitplane Inc. Michael C. Wussow Vice President and General Manager Bitplane Inc.
Cell Phone: 651-336-4600 Fax: 866-691-9112 Toll Free: 1-888-3D-BITPX (332-4879) Visit Our Web Site At: www.bitplane.com
==============================Original Headers============================== 19, 32 -- From mike-at-bitplane.com Fri Jan 25 09:11:08 2008 19, 32 -- Received: from host55a.simplicato.com (host55a.simplicato.com [207.99.47.55]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0PFB85w023696 19, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 09:11:08 -0600 19, 32 -- Received: from localhost (localhost.simplicato.com [127.0.0.1]) 19, 32 -- by host55a.simplicato.com (Postfix) with ESMTP id 20D48B23581 19, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 10:11:08 -0500 (EST) 19, 32 -- Received: from host55a.simplicato.com ([127.0.0.1]) 19, 32 -- by localhost (host55a.simplicato.com [127.0.0.1]) (amavisd-new, port 10024) 19, 32 -- with ESMTP id 49479-02 for {Microscopy-at-microscopy.com} ; 19, 32 -- Fri, 25 Jan 2008 10:11:07 -0500 (EST) 19, 32 -- Received: from MikeLaptop (71-34-18-33.mpls.qwest.net [71.34.18.33]) 19, 32 -- by host55a.simplicato.com (Postfix) with ESMTP id 8B15AB234F1 19, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 10:11:07 -0500 (EST) 19, 32 -- Reply-To: {mike-at-bitplane.com} 19, 32 -- From: "Michael C. Wussow" {mike-at-bitplane.com} 19, 32 -- To: {Microscopy-at-microscopy.com} 19, 32 -- Subject: Job Posting 19, 32 -- Date: Fri, 25 Jan 2008 09:10:58 -0600 19, 32 -- Organization: Bitplane Inc. 19, 32 -- Message-ID: {030e01c85f64$7d911e90$78b35bb0$-at-com} 19, 32 -- MIME-Version: 1.0 19, 32 -- Content-Type: text/plain; 19, 32 -- charset="iso-8859-1" 19, 32 -- X-Mailer: Microsoft Office Outlook 12.0 19, 32 -- Thread-Index: AchfZDp2miYtgTJARIKUoKPxVIvtaw== 19, 32 -- Content-Language: en-us 19, 32 -- x-cr-hashedpuzzle: CMEP C/eL EBo8 EIwB FBH9 Gi0j H4KJ H91A IJTk IVtl JQmz JuZ1 Koc5 K8MC LPso LmMF;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{8504F353-188F-48D3-95C7-D470356798A6};bQBpAGsAZQBAAGIAaQB0AHAAbABhAG4AZQAuAGMAbwBtAA==;Fri, 25 Jan 2008 15:10:55 GMT;SgBvAGIAIABQAG8AcwB0AGkAbgBnAA== 19, 32 -- x-cr-puzzleid: {8504F353-188F-48D3-95C7-D470356798A6} 19, 32 -- X-Virus-Scanned: by amavisd-new at simplicato.com 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0PFB85w023696 ==============================End of - Headers==============================
We're having some beam instability problems with our tool. The local fixit person would like to rule out the high voltage as the cause. He asked me to find an HV tank to substitute. Is it possible someone has a tank (or other parts) sitting around, gathering dust?
Perhaps someone has had this problem before. After turning on the beam, the current drops. It wavers and then comes back only to repeat after a short time. The time periods are approximate; 20 minutes at first, and 10 minutes in the repeat cycle. If the beam is turned off for several minutes while the HV is ramped up, there will be a longer time period before the current drops again.
TIA, Annie --
+++++++++++++++++++++++++++++
R. Ann Bliss, Technologist Chemistry Materials, Earth and Life Sciences Materials Science and Technology Division Lawrence Livermore National Laboratory
_____________________________
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea-at-ncmir.ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: andrea-at-ncmir.ucsd.edu Name: Andrea Thor
Organization: UCSD
Title-Subject: [Filtered] problems cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface
Question: I am having some problems in cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface. I heard this is not uncommon when pushing the section thickness to the extreme (such as 3-5 microns). We usually deal with the situation by refacing the block after each thick section, but this of course would make true "serial" sectioning impossible. The blocks I am working with are either cardic left ventricle or striatal tissue embedded in Durcupan.
If anyone has tried or has a protocol or techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
This Question was submitted to Ask-A-Microscopist by (davefissell-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, January 26, 2008 at 04:33:33 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both davefissell-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: davefissell-at-yahoo.com Name: David Fissell
Organization: CSU-DH
Education: Graduate College
Location: Brownsburg, IN, USA
Title: Microscope Ergonomics
Question: Visual inspection with a microscope is a common work situation for many industries. It is believed, poor ergonomic design of workstations where work demands involving a microscope are high (4-6 hours/work day) result in substandard performance. My question: Are you familiar with any research/industry guidelines supporting or countering this believe? Do you know of any ergonomic guidelines/standards prescribed for microscopist workstation design? Thank You David PS: My specific area of research is the impact on visual perception in industrial behavioral situations.
I am not sure of any specific health and safety regulations issued by any any safety organizations.
But I would suggest that appropriate or similar regulations such as would be useful: 1. The provision, use and maintenance of workplace equipment (PUWER 1998 UK safety regulations) 2. Display Screen Equipment (computers and similar instruments) regulations (DSE 2002 UK safety regulations) 3. and specific safety handling regulations for specimens such as chemical, biological and microbiological where appropriate in the lab.
I did a quick Google search (see below) and found a few industry and university websites. They all seem to consider ergonomics, optimal operation, good maintenance, environment, work activity patterns and nature of the specimen and any chemicals as most important. Most users seem to prefer binocular eyepieces with a good range of easy to use adjustments (eg dioptre correction and interocular distance.
I hope this helps and sorry about all the UK safety references but I'm sure there will be similar US regulations.
Good luck
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: davefissell-at-yahoo.com
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Email: z.zhou-at-sheffield.ac.uk Name: Zoe Zhou
Organization: The University of Sheffield
Title-Subject: [Filtered] FIB
Question: I'm using a FEI quanta 200 3D focused ion beam microscope. I find tricky to control and understand the carbon deposition quality. I wonder what the gas injection process is while doing either C or Pt deposition pads. Is it an electron or ion plasma enhanced chemical vapour deposition process? Can anybody lead me to some related literatures?
David, This particular information is considered more critical in the area of Patholgy, either in pharmaceutical or hospital settings (or associated contract work). Some pathologists are expected to spend 40 hours a week doing nothing but reviewing thousands of microscope slides. It is one case where ergonomic statistics have definitely been researched extensively, since improving efficiency by a mere 5% can have quite an impact.
I personally do not have a source for this information, but a pathology oriented website probably could help.
Good luck, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: davefissell-at-yahoo.com [mailto:davefissell-at-yahoo.com] Sent: Saturday, January 26, 2008 10:57 AM To: Sobocinski, Gregg
This Question was submitted to Ask-A-Microscopist by (davefissell-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, January 26, 2008 at 04:33:33 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both davefissell-at-yahoo.com as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: davefissell-at-yahoo.com Name: David Fissell
Organization: CSU-DH
Education: Graduate College
Location: Brownsburg, IN, USA
Title: Microscope Ergonomics
Question: Visual inspection with a microscope is a common work situation for many industries. It is believed, poor ergonomic design of workstations where work demands involving a microscope are high (4-6 hours/work day) result in substandard performance. My question: Are you familiar with any research/industry guidelines supporting or countering this believe? Do you know of any ergonomic guidelines/standards prescribed for microscopist workstation design? Thank You David PS: My specific area of research is the impact on visual perception in industrial behavioral situations.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (agata.sena-at-hotmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 28, 2008 at 05:24:58 ---------------------------------------------------------------------------
Email: agata.sena-at-hotmail.com Name: Agata
Organization: Materials Division
Education: Graduate College
Location: Brazil
Question: Dear all:
I'm characterizing gold tips by SEM/EDS. We are measuring the end of the tips and some of them have a deposit. I observed Si, S, Cu and Zn as contaminant in the EDS spectra of the deposit. The measurements are made on a FEI-Nano Lab and EDAX equipments. I didn't see any carbon. Is it possible thta these impurities be coming from SEM chamber by e-beam exposion?
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Jan 28 08:27:32 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SERWxS027504 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:27:32 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1635869rvb.30 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=FYtPDGqDD30Dzu88HqWm+shucNHLy9hZt3NVOdJhC6Y=; 3, 27 -- b=X5AcftGmXmdCJBzp/o3OZ9ZOoRDE+QKF5eGUHj9zpkztc2vC/9FletPiPi4lR6N0a8G16szwIYtQZhSekmcckT/jBZaJkGaM0xM8KtyK2+OXAGtJgETHVpq+MhH1F7CRl198We4dFvKpuXVnx4wtIaYwq9p58M2AR0kHrUc5lD8= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=U+sLkfSEw5VwotTATdOJGxwxBbZ4F4rNGetrN/vO8SVtQ9W2vd7yf1w0LXGBiQgH2Ap4Ml04FjWEJDWlF7KQFApbF/4aTLyQRQSuCpt7iXdA03QFHVaxlQ1IHGcHBPSOPa8AY/Hv3rbEsXmRuZI5NXv6UQ7vO9mP271emE0D57s= 3, 27 -- Received: by 10.140.180.42 with SMTP id c42mr3461545rvf.145.1201530449361; 3, 27 -- Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20801280627xe92e3b5y529ee11de5ab638b-at-mail.gmail.com} 3, 27 -- Date: Mon, 28 Jan 2008 09:27:29 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Chiller pump and lifting power. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I'm sure the chiller manufacturer can answer this question for you. Our own experience in doing exactly the same thing was that we were able to run our (Haskris) chiller lines through the ceiling and move the chiller two rooms away----about 20 feet as the crow walks---with no problems whatsoever. However, when we needed that room for a new scope and moved the chiller again, we went a room too far with that extra 10-odd feet. The TEM began shutting down its lenses at frequent intervals and our JEOL engineer quickly figured out that the water flow was reduced to the point that we were running right at the edge of the water's ability to cool the lenses. A few degrees and the scope would shut them down. We were due to install a new TEM anyway, so he tweaked the temperature settings to allow a slight extra increase in temperature before it shut down, and we limped over the finish line until the new scope came.
Another caution--- when the lines run overhead be very careful to check for the development of leaks. Water spraying down on equipment can be fundamentally different that water puddling on the floor (we've had both happen and we've been lucky each time). Copper lines have the nasty tendency to occasionally develop pinhole leaks from being etched internally by distilled water.
So, yes, problems are possible, but it will be a function of chiller pumping capacity and distance from the scope. Again, check with the manufacturer.
Good luck!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 28, 2008 8:28 AM To: Tindall, Randy D.
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Jan 28 08:27:32 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SERWxS027504 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:27:32 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1635869rvb.30 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=FYtPDGqDD30Dzu88HqWm+shucNHLy9hZt3NVOdJhC6Y=; 3, 27 -- b=X5AcftGmXmdCJBzp/o3OZ9ZOoRDE+QKF5eGUHj9zpkztc2vC/9FletPiPi4lR6N0a8G16s zwIYtQZhSekmcckT/jBZaJkGaM0xM8KtyK2+OXAGtJgETHVpq+MhH1F7CRl198We4dFvKpuX Vnx4wtIaYwq9p58M2AR0kHrUc5lD8= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer-encoding:content-disposition; 3, 27 -- b=U+sLkfSEw5VwotTATdOJGxwxBbZ4F4rNGetrN/vO8SVtQ9W2vd7yf1w0LXGBiQgH2Ap4Ml 04FjWEJDWlF7KQFApbF/4aTLyQRQSuCpt7iXdA03QFHVaxlQ1IHGcHBPSOPa8AY/Hv3rbEsX mRuZI5NXv6UQ7vO9mP271emE0D57s= 3, 27 -- Received: by 10.140.180.42 with SMTP id c42mr3461545rvf.145.1201530449361; 3, 27 -- Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20801280627xe92e3b5y529ee11de5ab638b-at-mail.gmail.com} 3, 27 -- Date: Mon, 28 Jan 2008 09:27:29 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Chiller pump and lifting power. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From TindallR-at-missouri.edu Mon Jan 28 08:46:08 2008 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SEk7ep008180 17, 26 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:46:08 -0600 17, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 26 -- Mon, 28 Jan 2008 08:46:07 -0600 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="US-ASCII" 17, 26 -- Subject: RE: [Microscopy] Chiller pump and lifting power. 17, 26 -- Date: Mon, 28 Jan 2008 08:46:07 -0600 17, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7318-at-UM-XMAIL08.um.umsystem.edu} 17, 26 -- In-Reply-To: {200801281428.m0SESQQF029299-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] Chiller pump and lifting power. 17, 26 -- Thread-Index: Achhugvw/AQrtpSuR16k2kxC70lBTgAAEj+A 17, 26 -- References: {200801281428.m0SESQQF029299-at-ns.microscopy.com} 17, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 17, 26 -- To: {kraftpiano-at-gmail.com} 17, 26 -- Cc: {microscopy-at-microscopy.com} 17, 26 -- X-OriginalArrivalTime: 28 Jan 2008 14:46:07.0452 (UTC) FILETIME=[83E359C0:01C861BC] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0SEk7ep008180 ==============================End of - Headers==============================
X-from a plumbing point of view, your latter comment is correct: if the inlet and outlet are at the same height as before, there is no extra consideration from running the hose up and down. If the piping system were open, the height of the loop would be a factor. If there is a difference in height between inlet and outlet, that must be taken into account.
However, the bigger concern in this case is the pressure drop due to the sheer length of tubing involved. Moving the chiller to another room and adding 15 to 20 feet just to loop over the wall adds a lot of extra flow restriction. Extra fittings may also be significant. Chemical engineers have tables of equivalent tube lengths for the many kinds of fittings. I have forgotten the details, but each fitting adds a substantial length (maybe up to a foot) to the effective length of the loop. It is good to keep them to a minimum.
The pressure drop can be handled by switching to a larger pump or by switching to a larger size hose. However, neither option is cheap. Consider it as you make your plans.
Warren Straszheim
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 28, 2008 8:28 AM To: wesaia-at-iastate.edu
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 10, 37 -- From wesaia-at-iastate.edu Mon Jan 28 09:48:00 2008 10, 37 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 10, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SFm0au032609 10, 37 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 09:48:00 -0600 10, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 10, 37 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m0SFlwXu021996; 10, 37 -- Mon, 28 Jan 2008 09:47:58 -0600 10, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 10, 37 -- id 4d3b_fa867f32_cdb7_11dc_91cd_00137253420a; 10, 37 -- Mon, 28 Jan 2008 09:44:57 -0600 10, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 10, 37 -- by despam-10.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m0SFlso2027001; 10, 37 -- Mon, 28 Jan 2008 09:47:56 -0600 10, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 37 -- Mon, 28 Jan 2008 09:46:35 -0600 10, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 37 -- Content-class: urn:content-classes:message 10, 37 -- MIME-Version: 1.0 10, 37 -- Content-Type: text/plain; 10, 37 -- charset="us-ascii" 10, 37 -- Subject: RE: [Microscopy] Chiller pump and lifting power. 10, 37 -- Date: Mon, 28 Jan 2008 09:46:36 -0600 10, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270277488F-at-maire.eng.iastate.edu} 10, 37 -- In-Reply-To: {200801281428.m0SESDkE028709-at-ns.microscopy.com} 10, 37 -- X-MS-Has-Attach: 10, 37 -- X-MS-TNEF-Correlator: 10, 37 -- Thread-Topic: [Microscopy] Chiller pump and lifting power. 10, 37 -- Thread-Index: AchhugSu2bTucrHASEWOYXRAREX59wABgtog 10, 37 -- References: {200801281428.m0SESDkE028709-at-ns.microscopy.com} 10, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 10, 37 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 10, 37 -- Cc: {kraftpiano-at-gmail.com} 10, 37 -- X-OriginalArrivalTime: 28 Jan 2008 15:46:35.0057 (UTC) FILETIME=[F61BF210:01C861C4] 10, 37 -- X-PMX-Version: 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2008.1.28.73554 10, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='__CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 10, 37 -- Content-Transfer-Encoding: 8bit 10, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0SFm0au032609 ==============================End of - Headers==============================
Like Malcolm, I know of no ergonomic guidelines that are specific to microscopy. However, folks in our lab have much experience in the areas of optical, AFM, SEM, TEM and microtomy. We receive generalized training in ergonomics at my work. However, I have significant personal experience with ergo problems and know first-hand how painful and long-lasting the problems can become.
Ergonomically healthy posture when using a stand-alone optical microscope, TEM or microtome, i.e. without a computer interface, is relatively straightforward to achieve. Good, relaxed posture is important. Ensure that a chair equipped with arm rests is available that allows each analyst to look through the oculars without hunching over or straining upward. Minimizing eye strain is essential for long periods of time at an optical microscope. Ensure that each analyst knows how to adjust the binoculars to ones interpupillary distance and focus for each eye. Try to minimize bright lighting above or around the microscope. Glare and too-bright ambient lighting can pose problems during extending work on a microscope or computer.
Unfortunately, interfacing a computer, monitor, keyboard and mouse turns a relatively ergonomically simple instrument into a nightmare for posture. Our internal ergo consultants have provided some help although they contend that computerization of microscopes generates hard-to-resolve problems. The problem that occurs when one integrates a computer with any type of microscope is that the microscope and computer, ergonomically benign tools when used correctly, each become difficult to use. It seems that one may choose to be comfortable at one or the other but not both. Trade-offs tend to be the rule. I suggest that one achieve the best ergo-friendly setup for the tool that is used most frequently. If one tends to spend most of the time at the microscope and only intermittently work at the computer, then design the ergonomics of the work area around the scope; the converse should be applied if the primary tool used during analyses is the computer.
Several key points should be made. Please note that repeated reaching or twisting means even several times during multiple short sessions at the scope. Ergo problems tend to be cumulative during a day or even days: Never repeatedly twist the body to reach a tool. Rather, roll the chair to the tool and assume good posture. It takes a little longer but will be worthwhile in the end. Avoid repeated reaching, i.e. extending ones arm, to perform a task. This can quickly lead to severe shoulder and neck pain. Take a short break each half hour on the scope. Spending a couple of minutes to stretch the hands, shoulders, back and neck can work wonders. Finally, be aware of minor aches and pains that didn't seem to be there before. A hand, thumb, arm, shoulder, back or neck that is even slightly stiff or sore should be stretched and rested before continuing extensive work on the scope. Varying the type of work done during the day can be a big help in minimizing or eliminating ergo problems. A set of cold eyes is often useful to recognize problems of which the individual may not aware.
Several guidelines that we have found helpful in the use of computers follow: The computer monitor should be adjustable for each, not most, microscopist. Set the monitor height such that the top of the monitor field is an inch or so higher than the persons eyes when looking straight ahead. Avoid looking up or down at a monitor. I have found that looking upward at a monitor may cause very significant issues with the neck and shoulders, sometimes in a very short period of time. Like monitors, keyboards and mouse(mouses, mice ?) should be adjustable for each (not most) microscopist. Avoid reaching or extending the arms to reach the keyboard or mouse. Adjustable keyboard articulating trays are very useful. The keyboard and mouse should be set to neutral posture such that the lower arms are horizontally orientally and the tops of the hands do not bend up or down. Consider using fitting the computer with a trackball in addition to or instead of a mouse. When selecting a trackball, look for one with the largest ball available. The ball should be one that is moved with the fingers and/or hands, not the thumb. I use the Kensington Expert Mouse (I know that other good trackballs exist). The ball is about the size of a billiard ball.
Keep in mind that solving and correcting ergo problems in microscopy labs often requires patience and perseverance.
Best regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
----- Forwarded by Gary M Brown/Baytown/ExxonMobil on 01/28/08 10:11 AM -----
malcolm.haswel l-at-sunderland.a c.uk To gary.m.brown-at-exxonmobil.com cc 01/28/08 05:10 AM Subject [Microscopy] Re: AskAMicroscopist: Microscope Ergonomics Please respond to malcolm.haswel l-at-sunderland.a c.uk
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
David
I am not sure of any specific health and safety regulations issued by any any safety organizations.
But I would suggest that appropriate or similar regulations such as would be useful: 1. The provision, use and maintenance of workplace equipment (PUWER 1998 UK safety regulations) 2. Display Screen Equipment (computers and similar instruments) regulations (DSE 2002 UK safety regulations) 3. and specific safety handling regulations for specimens such as chemical, biological and microbiological where appropriate in the lab.
I did a quick Google search (see below) and found a few industry and university websites. They all seem to consider ergonomics, optimal operation, good maintenance, environment, work activity patterns and nature of the specimen and any chemicals as most important. Most users seem to prefer binocular eyepieces with a good range of easy to use adjustments (eg dioptre correction and interocular distance.
I hope this helps and sorry about all the UK safety references but I'm sure there will be similar US regulations.
Good luck
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: davefissell-at-yahoo.com
Hi,
The short answer is that it takes only 5 PSI of pressure for every 10 feet (one story tall) of vertical head or drop. So you only need 5 PSI extra to push "up hill". I am sure you have that much pressure. Suppose the chiller output is 50 PSI. The pressure at the top of the pipe would be the output of the chiller under normal SEM conditions minus 5 PSI. You will gain that 5 PSI back on the drop side of the inverted U tube. So the pressure at the SEM input should still be 50 PSI. If it's lower than 50, you could still have an air lock (see below). If you keep your bypass open, then the pressure will drop. Keep it closed and the SEM on-line during pressure measurements. The pressure losses from the delivery lines should be minor unless you use really small diameter pipes or have a lot of bends. Stick with 1/2" ID plastic, not copper. Use pipe rated for at least 100 PSI.
Your problem might be in eliminating the initial inverted U loop's air lock at the top of the loop. You might consider installing a tee and globe valve at the top of the loop to bleed off *most* of the tapped air.
Paul
At 08:28 AM 1/28/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 5, 28 -- From beaurega-at-westol.com Mon Jan 28 12:52:53 2008 5, 28 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.97.73]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SIqpUH006309 5, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 12:52:52 -0600 5, 28 -- X-Winbeam-MailScanner-Watermark: 1202151145.04289-at-xq9W19+GjPQfEER6/X3TDg 5, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 5, 28 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id m0SIqKlJ020496 5, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 13:52:23 -0500 5, 28 -- Received: (qmail 15267 invoked by uid 89); 28 Jan 2008 18:52:21 -0000 5, 28 -- Received: from pitts-69-72-12-130.dynamic-dialup.coretel.net (HELO millenium) (69.72.12.130) 5, 28 -- by mail.winbeam.com with SMTP; 28 Jan 2008 18:52:21 -0000 5, 28 -- Message-Id: {3.0.6.32.20080128135230.007d4720-at-pop3.norton.antivirus} 5, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 5, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 5, 28 -- Date: Mon, 28 Jan 2008 13:52:30 -0500 5, 28 -- To: microscopy-at-microscopy.com 5, 28 -- From: Beaurega {beaurega-at-westol.com} 5, 28 -- Subject: Re: [Microscopy] Chiller pump and lifting power. 5, 28 -- In-Reply-To: {200801281428.m0SES07q028210-at-ns.microscopy.com} 5, 28 -- Mime-Version: 1.0 5, 28 -- Content-Type: text/plain; charset="us-ascii" 5, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 5, 28 -- X-MailScanner-ID: m0SIqKlJ020496 5, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 5, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 5, 28 -- SpamAssassin (not cached, score=-0.5, required 4, autolearn=not spam, 5, 28 -- BAYES_00 -0.50) 5, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
Hi, we have recently started to work on corpus callosum formation in the developing mamalian forebrain. First we performed a pilot study to see the callosol axon projections on E 14 and E 17 mouse foetus brain regarding the reference studies. We could see the callosal axons on the coronal sections as shown in the references . But we decided to run the study with rat foetuses instead of mice because of the lack of the facilities. However we could not find any spesific timing information in the literature about CC formation in rats . We wonder when CC spesificly begins and ends to form during the rat gestation and also where we suppose to see the growing callosal axons on E 14, 15 and 17 on the coronal histological sections in rats.
I would greatly appreciate if you could inform me about these issues .
Thank you in advance for any information you can suggest me.
Yours Sincerely
Dr. Necat Yilmaz Mersin Universitesi Tip Fakultesi Histoloji ve Embriyoloji Anabilim Dali
==============================Original Headers============================== 12, 31 -- From nyilmaz-at-mersin.edu.tr Mon Jan 28 13:12:20 2008 12, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3]) 12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SJCJ7D019510 12, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 13:12:20 -0600 12, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 12, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 7702B3647A3; 12, 31 -- Mon, 28 Jan 2008 21:12:37 +0200 (EET) 12, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 12, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 12, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 12, 31 -- with ESMTP id oL8gEgGfzLXP; Mon, 28 Jan 2008 21:12:08 +0200 (EET) 12, 31 -- Received: from NEJAT1 (unknown [88.227.7.183]) 12, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 2FAFD36479E; 12, 31 -- Mon, 28 Jan 2008 21:12:08 +0200 (EET) 12, 31 -- Message-ID: {004301c861e1$a2b42530$0401a8c0-at-NEJAT1} 12, 31 -- From: "Nejat" {nyilmaz-at-mersin.edu.tr} 12, 31 -- To: {histonet-at-lists.utsouthwestern.edu} , 12, 31 -- "EM-Mail Group" {Microscopy-at-microscopy.com} 12, 31 -- Subject: Rat corpus callosum formation 12, 31 -- Date: Mon, 28 Jan 2008 21:10:57 +0200 12, 31 -- MIME-Version: 1.0 12, 31 -- Content-Type: text/plain; 12, 31 -- format=flowed; 12, 31 -- charset="windows-1254"; 12, 31 -- reply-type=original 12, 31 -- Content-Transfer-Encoding: 7bit 12, 31 -- X-Priority: 3 12, 31 -- X-MSMail-Priority: Normal 12, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 12, 31 -- Disposition-Notification-To: "Nejat" {nyilmaz-at-mersin.edu.tr} 12, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Anyone know of a plugin for the free image analysis program ImageTool that will allow it perform automatic particle size and aspect ratio(height to diameter) calculations? I understand this program can use Photoshop plugins also. Thanks in advance.
Gary M. Easton
Scanners Corporation Independent SEM Service 30 years experience Cambridge SEM's our specialty 410-857-7633
==============================Original Headers============================== 7, 21 -- From garyeaston-at-scannerscorp.com Mon Jan 28 15:34:33 2008 7, 21 -- Received: from omr14.networksolutionsemail.com (omr14.networksolutionsemail.com [205.178.146.64]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SLYXkX006297 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 15:34:33 -0600 7, 21 -- Received: from mail.networksolutionsemail.com (ns-omr14.mgt.hosting.dc2.netsol.com [10.49.6.77]) 7, 21 -- by omr14.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id m0SLYXEX000470 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 16:34:33 -0500 7, 21 -- Received: (qmail 31552 invoked by uid 78); 28 Jan 2008 21:34:32 -0000 7, 21 -- Received: from unknown (HELO ?127.0.0.1?) (70.22.75.237) 7, 21 -- by ns-omr14.lb.hosting.dc2.netsol.com with SMTP; 28 Jan 2008 21:34:32 -0000 7, 21 -- Message-ID: {479E4A67.2020901-at-scannerscorp.com} 7, 21 -- Date: Mon, 28 Jan 2008 16:34:31 -0500 7, 21 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com} 7, 21 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 7, 21 -- MIME-Version: 1.0 7, 21 -- To: Microscopy Society of America {microscopy-at-microscopy.com} 7, 21 -- Subject: Question about ImageTool 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Antivirus: avast! (VPS 080128-0, 01/28/2008), Outbound message 7, 21 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
On Jan 28, 2008, at 6:27 AM, kraftpiano-at-gmail.com wrote:
} I finally got sick of the noise and heat being thrown off by my water } chiller system for the SEM, so I decided to move it to another room. } Before I do this, though, the water lines have to be run up into the } ceiling, over about 20 feet, then down to the microscope again. I've } taken into consideration the connection end and plumbed in a valve } system so I have a cut-off to run water through the chiller and piping } without circulating it through the SEM, but I'm still wondering about } the lifting power of the pump. Does the power required to lift the } water lower the flow rate, or does the drop on the other end create } enough of a siphon effect to cancel out any effects of the lift? } } I seem to be able to reason this one out either way, depending on } which outcome I'm looking for...
Dear Justin, The chillers for the high-voltage scope in Albany were located in a sub-sub basement about 10 meters below the lowest point of the scope, and the water distribution manifold for the lenses was located another 5 meters above that. There was no difficulty supplying water to any part of the scope. The pump on the chiller pushed water up to the manifold, so the height is not a problem, and, as long as there is no way air can get into the lines, each liter of water pushed into the line forces one liter to be pushed into the tank, so the water should circulate the same way regardless of the height it is raised to. The flow rate can be affected by the length of the lines due to viscosity; i.e., the pump must provide enough power to overcome the resistance due to the friction of the water flowing through the lines, which is higher for longer and thinner lines. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Mon Jan 28 15:35:53 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SLZrHi007990 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 15:35:53 -0600 6, 22 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 85A541B91D 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 13:35:51 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 12DF013E70 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 13:35:48 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200801281427.m0SERjMg027658-at-ns.microscopy.com} 6, 22 -- References: {200801281427.m0SERjMg027658-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {A72D0999-8714-49D5-8162-0D71CB57ABB7-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] Chiller pump and lifting power. 6, 22 -- Date: Mon, 28 Jan 2008 13:35:54 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
We have been having major problems with our in-line tap water filters becoming clogged (sometimes on a bi-weekly basis). These string-filters remove sediment from the tap water that is used to remove heat from our closed-loop water chillers. Our Physical Plant has not been able to locate the source of the sludge and we end up paying them several hundred dollars to come and change two sets of filters.
Does anyone know of a large capacity (self-cleaning) filtration system that could be used on the water coming into our small, microscopy building? Since our major use of water is probably the water chillers, it may be more advantageous to go this route rather than relying on the small, under-sink string filters.
Thank you,
John Bozzola -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question was submitted to Ask-A-Microscopist by (august04-at-verizon.net) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 28, 2008 at 18:36:19 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both august04-at-verizon.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: august04-at-verizon.net Name: Charles Pique
Organization: None
Education: Graduate College
Location: Charleston, West Virginia, USA
Title: Brownian motion
Question: What magnification and conditions are needed to see the brownian motion?
As I recall, Brownian motion is in part due to the density and temperature of particles. The differentiation of particles is important from large objects (Gouy, 1889). The smaller the particles, the more impact external forces have on them. This includes thermal effects.
I think that the basic theory is formulated on particles in a fluid. If one takes this literally, one could collect rotifers and examine them under a microscope at perhaps 100X or less and study their movement. Then, increase the temperature of their medium and the movement will increase. The point is that there is no distinct and definitive direction for movement in either of the thermal conditions. Movement is random. There is no distinct vector for movement regardless of temperature.
In particular, the length of the movement is infinite over any length of interval. Being rather impossible, Brownian motion is purely an abstract concept. This is based on the inability of a particle to move an infinite distance in a finite length of time. Einstein had some publications about Brownian motion. You might do some research on/for these.
As an aside, some have identified the movement of employees in a company as Brownian motion...moving here and there to show activity but with no actual production output. But that is a stretch (?).
Let us know what you find out.
gary g
At 04:47 PM 1/28/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Mon Jan 28 19:30:09 2008 9, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0T1U9ww030590 9, 20 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 19:30:09 -0600 9, 20 -- Message-Id: {200801290130.m0T1U9ww030590-at-ns.microscopy.com} 9, 20 -- Received: (qmail 2377 invoked from network); 28 Jan 2008 17:30:08 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 2374, pid: 2375, t: 0.1109s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by qsmtp1 with SMTP; 28 Jan 2008 17:30:08 -0800 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Mon, 28 Jan 2008 17:30:02 -0800 9, 20 -- To: august04-at-verizon.net 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: Brownian motion 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200801290047.m0T0lr1f019415-at-ns.microscopy.com} 9, 20 -- References: {200801290047.m0T0lr1f019415-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20F23A01 ==============================End of - Headers==============================
My swimming pool has some very large filters and I can clean them myself in a half hour. It would require shutting off the microscope, though. Better to locate the source of sludge.
John Mardinly,
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Monday, January 28, 2008 2:06 PM To: Mardinly, John
We have been having major problems with our in-line tap water filters becoming clogged (sometimes on a bi-weekly basis). These string-filters remove sediment from the tap water that is used to remove heat from our closed-loop water chillers. Our Physical Plant has not been able to locate the source of the sludge and we end up paying them several hundred dollars to come and change two sets of filters.
Does anyone know of a large capacity (self-cleaning) filtration system that could be used on the water coming into our small, microscopy building? Since our major use of water is probably the water chillers, it may be more advantageous to go this route rather than relying on the small, under-sink string filters.
Thank you,
John Bozzola -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 28, 2008 6:28 AM To: Mardinly, John
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Jan 28 08:27:32 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SERWxS027504 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:27:32 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1635869rvb.30 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=FYtPDGqDD30Dzu88HqWm+shucNHLy9hZt3NVOdJhC6Y=; 3, 27 -- b=X5AcftGmXmdCJBzp/o3OZ9ZOoRDE+QKF5eGUHj9zpkztc2vC/9FletPiPi4lR6N0a8G16s zwIYtQZhSekmcckT/jBZaJkGaM0xM8KtyK2+OXAGtJgETHVpq+MhH1F7CRl198We4dFvKpuX Vnx4wtIaYwq9p58M2AR0kHrUc5lD8= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer-encoding:content-disposition; 3, 27 -- b=U+sLkfSEw5VwotTATdOJGxwxBbZ4F4rNGetrN/vO8SVtQ9W2vd7yf1w0LXGBiQgH2Ap4Ml 04FjWEJDWlF7KQFApbF/4aTLyQRQSuCpt7iXdA03QFHVaxlQ1IHGcHBPSOPa8AY/Hv3rbEsX mRuZI5NXv6UQ7vO9mP271emE0D57s= 3, 27 -- Received: by 10.140.180.42 with SMTP id c42mr3461545rvf.145.1201530449361; 3, 27 -- Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20801280627xe92e3b5y529ee11de5ab638b-at-mail.gmail.com} 3, 27 -- Date: Mon, 28 Jan 2008 09:27:29 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Chiller pump and lifting power. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 12, 33 -- From john.mardinly-at-intel.com Mon Jan 28 19:55:18 2008 12, 33 -- Received: from mga09.intel.com (mga09.intel.com [134.134.136.24]) 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0T1tIww016745 12, 33 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 19:55:18 -0600 12, 33 -- Received: from fmsmga002.fm.intel.com ([10.253.24.26]) 12, 33 -- by orsmga102.jf.intel.com with ESMTP; 28 Jan 2008 17:55:05 -0800 12, 33 -- X-ExtLoop1: 1 12, 33 -- X-IronPort-AV: E=Sophos;i="4.25,265,1199692800"; 12, 33 -- d="scan'208";a="293828247" 12, 33 -- Received: from orsmsx335.jf.intel.com ([10.22.226.40]) 12, 33 -- by fmsmga002.fm.intel.com with ESMTP; 28 Jan 2008 17:53:06 -0800 12, 33 -- Received: from orsmsx423.amr.corp.intel.com ([10.22.226.104]) by orsmsx335.jf.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 12, 33 -- Mon, 28 Jan 2008 17:54:04 -0800 12, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 33 -- Content-class: urn:content-classes:message 12, 33 -- MIME-Version: 1.0 12, 33 -- Content-Type: text/plain; 12, 33 -- charset="us-ascii" 12, 33 -- Subject: RE: [Microscopy] Chiller pump and lifting power. 12, 33 -- Date: Mon, 28 Jan 2008 17:54:03 -0800 12, 33 -- Message-ID: {36C438BD1408B1499A19BA5730AAFC63AE0BF1-at-orsmsx423.amr.corp.intel.com} 12, 33 -- In-Reply-To: {200801281427.m0SERiTd027631-at-ns.microscopy.com} 12, 33 -- X-MS-Has-Attach: 12, 33 -- X-MS-TNEF-Correlator: 12, 33 -- Thread-Topic: [Microscopy] Chiller pump and lifting power. 12, 33 -- Thread-Index: AchhufN2O1HAfgawS0G1eCfRnwMCPQAX7MAA 12, 33 -- References: {200801281427.m0SERiTd027631-at-ns.microscopy.com} 12, 33 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 12, 33 -- To: {kraftpiano-at-gmail.com} 12, 33 -- Cc: {Microscopy-at-msa.microscopy.com} 12, 33 -- X-OriginalArrivalTime: 29 Jan 2008 01:54:04.0532 (UTC) FILETIME=[D3B07340:01C86219] 12, 33 -- Content-Transfer-Encoding: 8bit 12, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0T1tIww016745 ==============================End of - Headers==============================
Our water line currently has one filter and no bypass; on days when I've had enough coffee to be especially lucid I can change a filter without the chillers noticing. However it is directly over a 208 3-phase outlet so while rushing to do the job fast, water drips and I end up standing in a pool of water while watching it drip into the outlet - not great.
I sketched out a plan (as yet unimplemented) for a system with 2 alternate branches so that the flow can be switched to an unused filter unit while the loop with the used filter is isolated and the filter replaced. I have planned a drain/flush spigot on the inlet side so I can flush the line upstream of the filters from time to time, or before a filter change. The standard filters are really cheap at McMaster-Carr (a couple of dollars, I think; I get a case at a time, we also have 'enriched' water). I can send the Prod# - we use a spun plastic (5um I think) instead of the string filters.
It is going to be hard to beat the economy of standard mass-produced filter units for a low volume application supporting some chillers, etc. If you have the usual 'before and after' pressure gages you can see the pressure drop developing across the active filter and change filters before trouble comes knocking.
Dale Callaham UMASS, Amherst
bozzola-at-siu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have been having major problems with our in-line tap water filters } becoming clogged (sometimes on a bi-weekly basis). These } string-filters remove sediment from the tap water that is used to } remove heat from our closed-loop water chillers. Our Physical Plant } has not been able to locate the source of the sludge and we end up } paying them several hundred dollars to come and change two sets of } filters. } } Does anyone know of a large capacity (self-cleaning) filtration } system that could be used on the water coming into our small, } microscopy building? Since our major use of water is probably the } water chillers, it may be more advantageous to go this route rather } than relying on the small, under-sink string filters. } } Thank you, } } John Bozzola
==============================Original Headers============================== 6, 20 -- From dac-at-research.umass.edu Mon Jan 28 20:03:23 2008 6, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0T23N8c003930 6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 20:03:23 -0600 6, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 6, 20 -- (authenticated bits=0) 6, 20 -- by race1.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m0T23MXu022800 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 6, 20 -- Mon, 28 Jan 2008 21:03:22 -0500 6, 20 -- Message-ID: {479E896E.8020000-at-research.umass.edu} 6, 20 -- Date: Mon, 28 Jan 2008 21:03:26 -0500 6, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 6, 20 -- MIME-Version: 1.0 6, 20 -- To: bozzola-at-siu.edu, Microscopy List {microscopy-at-microscopy.com} 6, 20 -- Subject: Re: [Microscopy] large capacity tap water filters 6, 20 -- References: {200801282211.m0SMB9Kk008641-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200801282211.m0SMB9Kk008641-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
In my experience a rock thin section consisting mostly of quartz will offer opportunities to see this at magnifications of about 400x and above - occasionally even lower. Quartz that is formed hydrothermally (by crystalization from silica-bearing water deep in the earth) typically contains fluid inclusions. These are visible as voids inside the quartz. Often they are not completely filled by liquid and the remaining space is occupied by a bubble of gas. Brownian motion will be apparent from the agitation of the bubble--- it will appear to be constantly jostled about by some unseen forces. If the heat from the microscope illuminator is not completely eliminated by a filter, the jostling will become more violent as the thin section warms up and decrease when the light is removed for a time.
You will need to view the thin section in transmitted light but it is not necessary to have an actual petrographic microscope to see the effect, a biological scope will do. Some other stone sections will exhibit two-phase (gas-liquid) inclusions. Calcite, for example may contain gas-liquid inclusions or liquid-liquid inclusions. The latter consist of immiscible liquids such as brine and petroleum. However, the low mass of a gas bubble in a fluid inclusion makes it a better candidate to observe the motion and quartz more often provides a clear view.
John Twilley Conservation Scientist
On Mon, 28 Jan 2008 19:46:21 -0500, {august04-at-verizon.net} wrote:
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I agree with Dale. You should change your own filters, if possible. I have seen the dual in-line filter and switching setups used in plants that he described. Get filters with a pressure relief button to make changing cartridges easier.
The real questions are, "What is plugging the (cartridge?) filter and what is the size of the 'dirt'? Once you know the ID, from TEM for example, then you might be able to find the source or cause.
McMaster-Carr shows a fiberglass unit very similar to a water softener. It is a 'sediment and dirt' filter unit with a back wash timer. It is item 9843K11, ~$400. It says, "Rated at 50 microns."
You could try to rent one from a water softener company with the option to buy. If you are lucky, a large dealer will have softener units used in apartments for a short periods of time that are quite new and half the price. I did that to get a year old iron filter that used KMnO4. I also bought a used water softener that had the resin and sand changed out by the dealer. Both worked just fine.
Paul
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Question: Does anyone happen to know the going price/value of a 14 year old Zeiss EM900 electron microscope? (My facility is planning to purchase a CCD camera for this particular scope, but then 2 or 3 years down the road, is planning on purchasing an entirely new scope altogether. In my humble opinion, it would make more sense to buy the new scope now, complete with the digital camera, so as not to try to retro-fit a camera we buy now for the Zeiss, onto a scope that may not be a Zeiss.) Thanks in advance for your time and perspectives. Rita Kenner
Hi Listers I am doing an analysis of some immuno-TEM. I am interested in the enrichment of gold particles on a membrane. I am probing a transmembrane protein with an IgG and then a secondary (gold labeled) IgG. What I am thinking is to count the gold particles that are within X nm of the membranes in question and the gold that is not within X distance of the membrane. Then define enrichment as;
(# gold within X nm of membrane)/(length of membrane * (2 * X)) __________________________________________________
(# gold particles in field)/(area of field)
My questions are; 1) does this make sense? 2) am I reinventing the wheel (I assume yes :-) 3) if 'yes' to #2, where should I go for info 4) (most important of all) what is the value of X?
Thank you David
==============================Original Headers============================== 6, 20 -- From Elliott-at-arizona.edu Tue Jan 29 06:32:29 2008 6, 20 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TCWSXR008708 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 29 Jan 2008 06:32:29 -0600 6, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 3F75D3D8D1E 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 29 Jan 2008 05:32:26 -0700 (MST) 6, 20 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7A9293D8D1C 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 29 Jan 2008 05:32:25 -0700 (MST) 6, 20 -- Message-Id: {BCDC9C19-A0F9-483B-A11B-C62646340EBB-at-arizona.edu} 6, 20 -- From: David Elliott {Elliott-at-arizona.edu} 6, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- Mime-Version: 1.0 (Apple Message framework v915) 6, 20 -- Subject: imuno-gold TEM analysis 6, 20 -- Date: Tue, 29 Jan 2008 05:32:19 -0700 6, 20 -- X-Mailer: Apple Mail (2.915) 6, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Any "scientific" light microscope will suffice. Just look at a suspension of bacteria at 40x or 100x objective (at 40x the bacteria are just very tiny spots but you can see them moving) and you will see them dancing around. If flagella are involved in the movement, they have a faster and more regular movement like turning in circles or rushing fast through the field of view. By extension, any particle with a size close to bacteria can be visualized as well, like the debris usually present in eucaryotic cell cultures, fine dusts...
Best regards,
Stephane
----- Original Message ---- X-from: "august04-at-verizon.net" {august04-at-verizon.net} To: nizets2-at-yahoo.com Sent: Tuesday, January 29, 2008 1:51:29 AM
This Question was submitted to Ask-A-Microscopist by (august04-at-verizon.net) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 28, 2008 at 18:36:19 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both august04-at-verizon.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: august04-at-verizon.net Name: Charles Pique
Organization: None
Education: Graduate College
Location: Charleston, West Virginia, USA
Title: Brownian motion
Question: What magnification and conditions are needed to see the brownian motion?
==============================Original Headers============================== 9, 11 -- From zaluzec-at-ultra5.microscopy.com Mon Jan 28 18:46:05 2008 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0T0k4im016746 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 18:46:05 -0600 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p06240801c3c427b741b0-at-[206.69.208.22]} 9, 11 -- Date: Mon, 28 Jan 2008 18:46:02 -0600 9, 11 -- To: microscopy-at-microscopy.com 9, 11 -- From: august04-at-verizon.net (by way of Ask-A-Microscopist) 9, 11 -- Subject: AskAMicroscopist: Brownian motion 9, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 20 -- From nizets2-at-yahoo.com Tue Jan 29 07:48:50 2008 22, 20 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) 22, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0TDmosY024424 22, 20 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 07:48:50 -0600 22, 20 -- Received: (qmail 49491 invoked by uid 60001); 29 Jan 2008 13:48:50 -0000 22, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 22, 20 -- s=s1024; d=yahoo.com; 22, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 22, 20 -- b=p7QLK/e7CXBp28SfC+cZrP3dtEsqnA0oM4wN9QcHL2YVcNa7kRcb+438Msy4sySyy21Bq8uqsuoYqWcd0rLTAwRtvK72Vn/rrX07VdJvU5gA3S+FaIhq0RkkkVS/f4xU9kD/ziFTRfSWs09xEILF/WX2/FIKbjX/gDJXkLex2To=; 22, 20 -- X-YMail-OSG: QdrD8V8VM1kb.qt_dur1QAiTJg_dMJ0WoeLWb7gY31xTxyaGDxAu9j_NlREIV1KIlNnSy0tVI7f7XYGHAj9UrNiftc7CsObCi_99yUHc57mkvRQ2W7N_Ped7r9nm5ZFadJ1yaxfXY9evarG99.sgeC2hWZDfLuZYp2vQV6TU1Lkf 22, 20 -- Received: from [80.122.101.100] by web37407.mail.mud.yahoo.com via HTTP; Tue, 29 Jan 2008 05:48:50 PST 22, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.160 22, 20 -- Date: Tue, 29 Jan 2008 05:48:50 -0800 (PST) 22, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 22, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: Brownian motion 22, 20 -- To: august04-at-verizon.net 22, 20 -- Cc: microscopy-at-microscopy.com 22, 20 -- MIME-Version: 1.0 22, 20 -- Content-Type: text/plain; charset=us-ascii 22, 20 -- Message-ID: {324504.48968.qm-at-web37407.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Email: tjzhang-at-umd.edu Name: Tim
Organization: UMD
Title-Subject: [Filtered] Help please: ESEM-E3
Question: Dear All,
There is an ESEM E3 in our lab that is over 15 years old but runs well.
Last week, I found the battery on CPU board runs out. I have ordered the battery on line but I need to re-load the "start software". I wonder who has this kind of software and how to send it to RAM memory on CUP board?
There were some recent postings regarding service and maintenance of older Ultracut and Ultracut E microtomes. I didn't see any responses providing resources for after-market service in the Midwest area, and so I'm raising the question again. Is there anyone reasonably near the Chicago area who provides service for both ambient and cryo units?
Thanks in advance,
Elaine
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont,IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 9, 27 -- From eschumacher-at-mccrone.com Tue Jan 29 08:46:00 2008 9, 27 -- Received: from pgp.mccrone.com (65-43-97-126.ded.ameritech.net [65.43.97.126]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TEk0A7007180 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:00 -0600 9, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 9, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 675481A8015 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:01 -0600 (CST) 9, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 9, 27 -- by pgp.mccrone.com (PGP Universal service); 9, 27 -- Tue, 29 Jan 2008 08:46:01 -0600 9, 27 -- X-PGP-Universal: processed 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="US-ASCII" 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Subject: After-Market Service for Ultracut Microtomes 9, 27 -- Date: Tue, 29 Jan 2008 08:45:54 -0600 9, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC04-at-MCCRONEMSG.tmg.mccrone.com} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: After-Market Service for Ultracut Microtomes 9, 27 -- Thread-Index: AchihaZa3fymcB7KR+ye8rnYL7E12A== 9, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 9, 27 -- To: {microscopy-at-microscopy.com} 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0TEk0A7007180 ==============================End of - Headers==============================
Hi Elaine, I use David Benjamin {dbenjamin-at-dbmicroservice.com} . He is based in the Atlanta area so this info may not seem helpful but Airtran has many direct flights to your area. He's a good serviceman for microtomes, cryostats, and microscopes.
best, Beth
On Jan 29, 2008, at 9:46 AM, eschumacher-at-mccrone.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings Fellow Microscopists, } } There were some recent postings regarding service and maintenance of } older Ultracut and Ultracut E microtomes. I didn't see any responses } providing resources for after-market service in the Midwest area, } and so } I'm raising the question again. Is there anyone reasonably near the } Chicago area who provides service for both ambient and cryo units? } } Thanks in advance, } } Elaine } } } Elaine Schumacher } Senior Research Scientist } McCrone Associates, Inc. } 850 Pasquinelli Drive } Westmont,IL 60559-5539 USA } 630-887-7100 (tel) } 630-887-7417 (fax) } E-mail: eschumacher-at-mccrone.com } Web Site: www.mccrone.com } } } } } ==============================Original } Headers============================== } 9, 27 -- From eschumacher-at-mccrone.com Tue Jan 29 08:46:00 2008 } 9, 27 -- Received: from pgp.mccrone.com } (65-43-97-126.ded.ameritech.net [65.43.97.126]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m0TEk0A7007180 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:00 } -0600 } 9, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) } 9, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 675481A8015 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:01 } -0600 (CST) } 9, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) } 9, 27 -- by pgp.mccrone.com (PGP Universal service); } 9, 27 -- Tue, 29 Jan 2008 08:46:01 -0600 } 9, 27 -- X-PGP-Universal: processed } 9, 27 -- Content-class: urn:content-classes:message } 9, 27 -- MIME-Version: 1.0 } 9, 27 -- Content-Type: text/plain; } 9, 27 -- charset="US-ASCII" } 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 27 -- Subject: After-Market Service for Ultracut Microtomes } 9, 27 -- Date: Tue, 29 Jan 2008 08:45:54 -0600 } 9, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC04-at-MCCRONEMSG.tmg.mccrone.com } } } 9, 27 -- X-MS-Has-Attach: } 9, 27 -- X-MS-TNEF-Correlator: } 9, 27 -- Thread-Topic: After-Market Service for Ultracut Microtomes } 9, 27 -- Thread-Index: AchihaZa3fymcB7KR+ye8rnYL7E12A== } 9, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} } 9, 27 -- To: {microscopy-at-microscopy.com} } 9, 27 -- Content-Transfer-Encoding: 8bit } 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m0TEk0A7007180 } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 21 -- From beth-at-plantbio.uga.edu Tue Jan 29 09:25:25 2008 6, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TFPOAA000611 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 09:25:25 -0600 6, 21 -- Received: from [128.192.26.46] ([128.192.26.46]) 6, 21 -- (authenticated user beth-at-plantbio.uga.edu) 6, 21 -- by dogwood.plantbio.uga.edu 6, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 6, 21 -- Tue, 29 Jan 2008 10:25:18 -0500 6, 21 -- Cc: microscopy microscopy {microscopy-at-microscopy.com} 6, 21 -- Message-Id: {EE74570B-F994-4E80-ADD5-9DC10280F656-at-plantbio.uga.edu} 6, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 6, 21 -- To: eschumacher-at-mccrone.com 6, 21 -- In-Reply-To: {200801291446.m0TEkbsK008814-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v915) 6, 21 -- Subject: Re: [Microscopy] After-Market Service for Ultracut Microtomes 6, 21 -- Date: Tue, 29 Jan 2008 10:26:27 -0500 6, 21 -- References: {200801291446.m0TEkbsK008814-at-ns.microscopy.com} 6, 21 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
I've got a surgeon who is working with human livers that are very hard from the embalming process and he had heard that microwaving tissue may soften up the tissue and make it easier to cut. I'm going to let him use my Biowave and try different wattages. I have never heard of this effect before. Has anybody out there heard of or actually know if hardened fixed tissue can be softened by microwaves. Thanks, all help is appreciated.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 4, 20 -- From tbargar-at-unmc.edu Tue Jan 29 09:29:00 2008 4, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TFT028004622 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 4, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 4, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 2FE894C084 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 (CST) 4, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 4, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id C51AF4C06E 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:28:58 -0600 (CST) 4, 20 -- Subject: Can hardened fixed tissue be "softened" by Microwave treatment? 4, 20 -- To: Microscopy-at-MSA.Microscopy.com 4, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 4, 20 -- Message-ID: {OF0B63F2D6.945FCCD8-ON862573DF.005434C9-862573DF.00550C8B-at-unmc.edu} 4, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 4, 20 -- Date: Tue, 29 Jan 2008 09:28:57 -0600 4, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/29/2008 09:28:58 4, 20 -- AM 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Brownian motion is readily demonstrated using small particles in an aqueous phase. For example, India ink (consisting of carbon black particles) can be observed under high magnification (500X and above) to see the random movement of the black particles. The particles seem to "shiver" or "quake" and sometimes move off in random directions. If you have phase contrast (or darkfield), this would enhance the viewing. If not, then try offsetting the substage condenser so that the light is coming in at an angle.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I am interested in buying a zeiss axioplan microscope, (late 80's early 90's) or later model axiotron if available. If anyone has one they would like to sell or know of a source I would greatly appreciate a reply. Off line is probably best. Cheers Pat
Patrick R Kelly Operations Manager Geotrack International Pty Ltd ABN16 006 821 209 37 Melville Road, Brunswick West, Victoria 3055 Australia Telephone: +613 93801077 Facsimile: +613 93801477 email: mail-at-geotrack.com.au web: http://www.geotrack.com.au
==============================Original Headers============================== 4, 24 -- From probe-at-geotrack.com.au Wed Jan 30 00:47:28 2008 4, 24 -- Received: from geotrack.com.au (mail.geotrack.com.au [203.222.101.142]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0U6lRLJ023731 4, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 00:47:28 -0600 4, 24 -- Received: from PAT (pat.geotrack.com.au [192.168.5.18]) 4, 24 -- by geotrack.com.au (Postfix) with SMTP id 8FFFB1C00151 4, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 17:47:11 +1100 (EST) 4, 24 -- Message-ID: {004201c8630b$fa2dba30$1205a8c0-at-geotrack.com.au} 4, 24 -- Reply-To: "Pat Kelly" {probe-at-geotrack.com.au} 4, 24 -- From: "Pat Kelly" {probe-at-geotrack.com.au} 4, 24 -- To: {microscopy-at-microscopy.com} 4, 24 -- Subject: zeiss axioplan microscope 4, 24 -- Date: Wed, 30 Jan 2008 17:47:27 +1100 4, 24 -- Organization: Geotrack International 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- format=flowed; 4, 24 -- charset="iso-8859-1"; 4, 24 -- reply-type=original 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I wish to purchase a flatbed scanner for TEM negatives. I have received recommendations for the Canon 9950F and the Epson V750-M. The former may no longer be available, as it is not listed on the Canon web site--only the 8800F is. Any suggestions would be welcome and highly appreciated. Vachik Hacopian
==============================Original Headers============================== 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 2008 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UDa95N022047 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 -0600 2, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id m0UDa8Jp027778 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 -0500 (EST) 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) 2, 18 -- Message-id: {fc.006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 2, 18 -- Subject: TEM - negative scanners 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) 2, 18 -- X-FC-SERVER-TZ: 15729388 2, 18 -- To: Microscopy-at-Microscopy.Com 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} 2, 18 -- MIME-Version: 1.0 2, 18 -- Content-Type: text/plain; charset=UTF-8 2, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We just changed the IGP pump of the gun chamber only 4 years after the installation of our Tecnai G20. This change was advised by a FEI engineer. We do not even have 400 working hours on this machine and I wondered if it was normal that we already have to change the pump. We work mainly with ultrathin sections of biological probes embedded in Epon. They are pretty contaminated with carbon, as evidenced by light circles remaining on the sections after even a very short illumination time (I am usually working at 120 kV, LaB6). I am wondering if this carbon contamination, evaporated by the electron beam, is not at least partly responsible for the dirtiness of the pumps (the pump of the column is bigger than the one for the gun, which would explain why it was not so dirty). If this is the case, perhaps a plasma cleaner would not only be a convenience for me but could bring a financial advantage for my boss (I know you see what I mean ;-)). What would be the cost of a plasma cleaner? What is your opinion on the question? Do you think that a plasma cleaner would increase the lifetime of the IGPs?
Best regards,
Stephane
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==============================Original Headers============================== 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 6, 18 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0UF0pGt005637 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 09:00:53 -0600 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 15:00:50 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.com; 6, 18 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:Message-ID; 6, 18 -- b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9wOTjIMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXfnLKDhyAOWq+S20/9pUAEHFJq8=; 6, 18 -- X-YMail-OSG: OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3IfmhgAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Wed, 30 Jan 2008 07:00:50 PST 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=us-ascii 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} ==============================End of - Headers==============================
Postdoctoral position at Northwestern University DFT and Electron Microscopy
A postdoctoral position is available at Northwestern University for someone with expertise in both DFT (Wien2k, Vasp, PWSCF) and transmission electron microscopy. The research will be in a number of different areas including charge density measurements at both surfaces and for bulk materials; structure and energies of surface reconstructions; oxides for use either as catalysts or in solid oxide fuel cells. The applicant must hold a recent Ph.D. in physics, chemistry, or materials science. Further requirements for this position are: (1) A good knowledge of electronic structure theory. (2) Experience with first-principles calculations such as Density Functional Theory. (3) Extensive hands-on experience with transmission electron microscopy and diffraction (4) Ability to communicate effectively with coworkers and collaborators. (5) Demonstrated ability to write high-quality manuscripts suitable for publication in peer-reviewed journals.
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 4, 27 -- From marksmsa-at-gmail.com Wed Jan 30 12:46:52 2008 4, 27 -- Received: from el-out-1112.google.com (el-out-1112.google.com [209.85.162.181]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UIkpuv025139 4, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 12:46:51 -0600 4, 27 -- Received: by el-out-1112.google.com with SMTP id z25so110171ele.4 4, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 10:46:51 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=VUPAIlevAYLo1qi15Tl9L4WgCnc923pGS7B9+XqMxdk=; 4, 27 -- b=b1xIIDcQclQCLwHMmJEymrfonRl5c0gXDr0SiX17t0Tet1dsOnh3lRPM0Xj3+t0DeF9+pHD/1UjVsR7ZrAy0PGs24lGbf3RO2OfIIVulahCI5IcK1qlpVDIVTg2D/UOej3J+Pi0/655XR8PzUhDEI4grr6G1Crm/6wNBAudrPNs= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=sX80fu+LLdH9157vrLZ9s3lfmJH/alUKBwXrdvhWdvKHh1ZdZ4W6prQPQt9C6Sb0ytDCrc4Nu/aNpxU7Pp1UOQ3oZZ/S6i+z25ohFrxiofIKgkeiscO46hKEVrtkQffRLlTRXFR1kscSSKZEwr4PjJC/1aOMZVEsRpqydcjmwEw= 4, 27 -- Received: by 10.142.84.3 with SMTP id h3mr584001wfb.34.1201718810259; 4, 27 -- Wed, 30 Jan 2008 10:46:50 -0800 (PST) 4, 27 -- Received: by 10.142.223.6 with HTTP; Wed, 30 Jan 2008 10:46:50 -0800 (PST) 4, 27 -- Message-ID: {e13ba6260801301046s104dacfhdc92b1b01dff683a-at-mail.gmail.com} 4, 27 -- Date: Wed, 30 Jan 2008 12:46:50 -0600 4, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- Subject: Postdoctoral Position 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Postdoctoral position at Northwestern University In-Situ Tribology at the Nanoscale
A postdoctoral position is available at Northwestern University for someone with expertise in mechanical properties at the nanoscale and transmission electron microscopy, and the ability to do materials modelling. The research will involve extending established dislocation and deformation models used for grain boundaries and interfaces in strong materials to weak interfaces relevant to tribological applications [1-3]. For instance, the work might involve testing the limits of applying conventional models for restricting the motion of dislocations due to point defects in the bulk to the problem of point defects at the interface of two sliding materials. The applicant must hold a recent Ph.D. in physics, chemistry, or materials science with a preference for materials science. Further requirements for this position are: (1) A good knowledge basic mechanical properties at the atomistic nanoscale. (2) Some experience with transmission electron microscopy and diffraction in order to collaborate with others doing more experimental work 3) Ability to communicate effectively with coworkers and collaborators. (5) Demonstrated ability to write high-quality manuscripts suitable for publication in peer-reviewed journals.
Interested applicants should contact L-marks -at- northwestern.edu and send a CV including the names of referees as well as a 1 page outline of how they might use core materials science mechanical property formalism's and theories to model tribological processes at the nanoscale?
1. A. P. Merkle and L. D. Marks, Tribology Letters 26 73 (2007) 2. A. P. Merkle and L. D. Marks, Philosophical Magazine Letters, 87, 527 (2007) 3. A. P. Merkal and L. D. Marks, Appl Phys Letts 90, 064101 (2007)
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 5, 27 -- From marksmsa-at-gmail.com Wed Jan 30 12:56:47 2008 5, 27 -- Received: from el-out-1112.google.com (el-out-1112.google.com [209.85.162.176]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UIulsO005392 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 12:56:47 -0600 5, 27 -- Received: by el-out-1112.google.com with SMTP id z25so113806ele.4 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 10:56:47 -0800 (PST) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=AI4O1eDs9HFTvWG0x2fklgT3b0p52FmTtm3slMXB0G0=; 5, 27 -- b=sYmAb8FlZAgtM2iDoYIlg0u/nSWcnjqUThppTCkPeBvAdEFI6i9MdWev1SUn0dRpCSRgdAmjuzpb9NNpsioM/G9Zkw9hUM5sSVXyi5ElJkpGu+ZtCzXsET2QrCOFQ96aFlGoOB6o+zC4P7Rpy6XSnbPXsGko6KoQqlGwrbC7MMA= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=vjbXqtQW/s8pfdARrDbsPtv4rbAfDE6WXe84dK3IDN9YLze5oZR9BE+x1yNDP3IWYipgXA9Tffa9/fWnyScQAizgq1zk2HhQYMJqhgO4kcBlbqkyKvr5FneTHnNOez0K0z5Gpq12WDgy0n3UPb9AHbm0I0xZjmh7FwJ0V4z8OvE= 5, 27 -- Received: by 10.142.246.8 with SMTP id t8mr600001wfh.8.1201719405750; 5, 27 -- Wed, 30 Jan 2008 10:56:45 -0800 (PST) 5, 27 -- Received: by 10.142.223.6 with HTTP; Wed, 30 Jan 2008 10:56:45 -0800 (PST) 5, 27 -- Message-ID: {e13ba6260801301056p6a7f29aas55134f1887f588e6-at-mail.gmail.com} 5, 27 -- Date: Wed, 30 Jan 2008 12:56:45 -0600 5, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 5, 27 -- To: microscopy {microscopy-at-microscopy.com} 5, 27 -- Subject: Postdoctoral Position in Nanoscale Tribology 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Here are the leads that were suggested for possible servicing of older Ultracut microtomes. You may find some of them to be useful for other types of equipment service as well.
Tek-Net, Jon Petz, Lakewood, NJ, 732-905-5530 They send a crate for shipment of the equipment, which you return to them when you get the repaired unit back.
David Benjamin, dbenjamin-at-dbmicroservice.com, Atlanta, GA
Sercomp International, www.sercompintl.com
Please note that I haven't investigated any of these resources yet for my specific Ultracut issues, but I wanted to share the information. Thanks very much to all who replied to my posting.
Regards,
Elaine
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont,IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Wed Jan 30 13:05:12 2008 11, 27 -- Received: from pgp.mccrone.com (65-43-97-126.ded.ameritech.net [65.43.97.126]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UJ5CcO018299 11, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 13:05:12 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id CE45A1A8013 11, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 13:05:12 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Wed, 30 Jan 2008 13:05:12 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Subject: Summary of Suggestions for Microtome Service 11, 27 -- Date: Wed, 30 Jan 2008 13:05:06 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC14-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Summary of Suggestions for Microtome Service 11, 27 -- Thread-Index: AchjcwalgYTpq17cQSK1HknLSVnvxg== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {microscopy-at-microscopy.com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0UJ5CcO018299 ==============================End of - Headers==============================
On Jan 30, 2008, at 7:01 AM, nizets2-at-yahoo.com wrote:
} We just changed the IGP pump of the gun chamber only 4 years after } the installation of our Tecnai G20. This change was advised by a } FEI engineer. } We do not even have 400 working hours on this machine and I } wondered if it was normal that we already have to change the pump. } We work mainly with ultrathin sections of biological probes } embedded in Epon. They are pretty contaminated with carbon, as } evidenced by light circles remaining on the sections after even a } very short illumination time (I am usually working at 120 kV, } LaB6). I am wondering if this carbon contamination, evaporated by } the electron beam, is not at least partly responsible for the } dirtiness of the pumps (the pump of the column is bigger than the } one for the gun, which would explain why it was not so dirty). If } this is the case, perhaps a plasma cleaner would not only be a } convenience for me but could bring a financial advantage for my } boss (I know you see what I mean ;-)). } What would be the cost of a plasma cleaner? } What is your opinion on the question? Do you think that a plasma } cleaner would increase the lifetime of the IGPs?
Dear Stephane, The IGP is on full time, so even though there have been few hours that the instrument was in use, the pump has been working for the entire 4 years. This is still a short period, especially since there are relatively few contaminants in the gun volume (or should be). The mechanism of ion pumps is that residual gas is ionized and the ions are accelerated by an electric field and become embedded in the getter, which can get filled to the extent that any additional ion incident on the getter will dislodge an ion that is already there. The lifetime, therefore, depends on how good the vacuum is and how large the surface of the getter is. Other things that can shorten pump lifetime are the presence of water in the column--which is not likely to be an issue for your usage and is more of a problem in cryoEM--and the formation of spurs on the getter surface. The indication for pump replacement is loss of performance, so if your vacuum is poor, where it had been good, then the pump should be replaced (assuming that a vacuum leak has not developed). I doubt that carbon is the problem, but I could be wrong about that. A plasma cleaner can remove contamination from the column, which will give you a cleaner instrument and a better vacuum, which, in turn, will increase pump lifetime, but I don't know whether that will be enough to cover the cost--I'll let the manufacturers of these systems answer that question. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Jan 30 14:17:14 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UKHEBK002305 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 14:17:14 -0600 6, 22 -- Received: from earth-dog.its.caltech.edu (earth-dog [192.168.1.3]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 0C1E21BAB8 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 12:17:02 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 0E7951BB11 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 12:16:59 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200801301501.m0UF17Bd005785-at-ns.microscopy.com} 6, 22 -- References: {200801301501.m0UF17Bd005785-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {94B58638-19FD-4442-A214-C1F9CFDB21DC-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] 6, 22 -- Date: Wed, 30 Jan 2008 12:17:06 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.4.5 ==============================End of - Headers==============================
I've been following this discussion with interest since I have never had an instrument with IGP's. I'm surprised to find that IGP's have a (sometimes rather) limited lifetime. When you say they need to be replaced, do you mean just the adsorptive surfaces or the entire sealed system? How much does this cost? A ballpark figure is fine. I need this information for planning and teaching purposes.
Thank you,
John Bozzola Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
==============================Original Headers============================== 4, 19 -- From bozzola-at-siu.edu Wed Jan 30 14:50:02 2008 4, 19 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UKo2r9015938 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 14:50:02 -0600 4, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136]) 4, 19 -- by cstmta3.siu.edu (Switch-3.3.0/Switch-3.3.0) with ESMTP id m0UKo0xV010337 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 14:50:01 -0600 (CST) 4, 19 -- Mime-Version: 1.0 4, 19 -- Message-Id: {a06240809c3c691fcef3f-at-[131.230.177.136]} 4, 19 -- In-Reply-To: {200801302018.m0UKIL32003821-at-ns.microscopy.com} 4, 19 -- References: {200801302018.m0UKIL32003821-at-ns.microscopy.com} 4, 19 -- Date: Wed, 30 Jan 2008 14:49:57 -0600 4, 19 -- To: Microscopy-at-msa.microscopy.com 4, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 4, 19 -- Subject: [Microscopy] Re: ion pump longevity 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 19 -- X-Spam-Score: 0.00% 4, 19 -- X-MASF: 0.00% 4, 19 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
Not seeing a discussion of bakeout, I'd bring that up.
New generation pumps have built-in bakeout coils and instrument vacuum connections also have a bakeout coil surrounding each one. These pumps are essential for high vacuum that is highly beneficial for LaB6 cathodes and especially critical for FE systems. For W systems, I have never seen an IGP unless it was set up to handle both W and LaB6.
Depending on the use of the system, eventually the gun chamber will become contaminated with gas and always does when the cathode or FE tip is changed. Part of the changeout process is to bakeout the gun chamber, IGPs and vacuum connections (the connections with a gazillion bolts like between IGP and column/chamber). During this time, isolation valves that seal off the column and the gun chamber are opened while baking so the main vacuum system pulls the junk out. When done, the valves are closed and IGPs start pulling the vacuum down further.
During the bakeout process, the junk in the Ta and Ti parts of the IGP also get dislodged and dumped. This can be a separate part of "recycling" an IGP that is staturated but not requiring repair. When the IGP needs repair/rebuild, the whole thing (minus magnet) is sent off for rebuild or exchange. The following list from Duniway gives some typical costs for this.
I have not seen prices or services for the newer IGPs.
Older tools that do not have bakeout coils built-in can still be baked using off the shelf heating coil tape. They are rated in watts, width and length and are controlled by a simple thermal feedback box. These units are typically around $500 or less and can save lots of bucks if a simple bakeout is all that is needed from time to time--and with cathode/filament change.
With bakeout when the gun is changed and with reasonable care, my experience is that IGPs last for many years...perhaps up to ten years of continuous service. But of course, there is always the flakey one that does develop a flake and trips the IGP power supply and must be replaced.
gary g.
At 12:51 PM 1/30/2008, you wrote:
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==============================Original Headers============================== 17, 20 -- From gary-at-gaugler.com Wed Jan 30 15:24:50 2008 17, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0ULOonG029294 17, 20 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 15:24:50 -0600 17, 20 -- Message-Id: {200801302124.m0ULOonG029294-at-ns.microscopy.com} 17, 20 -- Received: (qmail 32761 invoked from network); 30 Jan 2008 13:24:49 -0800 17, 20 -- Received: by simscan 1.1.0 ppid: 32758, pid: 32759, t: 0.1345s 17, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 17, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 17, 20 -- by smtp1 with SMTP; 30 Jan 2008 13:24:49 -0800 17, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 17, 20 -- Date: Wed, 30 Jan 2008 13:24:48 -0800 17, 20 -- To: bozzola-at-siu.edu 17, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 17, 20 -- Subject: Re: [Microscopy] Re: ion pump longevity 17, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 17, 20 -- In-Reply-To: {200801302051.m0UKpVjW018590-at-ns.microscopy.com} 17, 20 -- References: {200801302051.m0UKpVjW018590-at-ns.microscopy.com} 17, 20 -- Mime-Version: 1.0 17, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-72B419C3 ==============================End of - Headers==============================
On Jan 30, 2008, at 12:50 PM, bozzola-at-siu.edu wrote:
} I've been following this discussion with interest since I have never } had an instrument with IGP's. I'm surprised to find that IGP's have a } (sometimes rather) limited lifetime. When you say they need to be } replaced, do you mean just the adsorptive surfaces or the entire } sealed system? How much does this cost? A ballpark figure is fine. I } need this information for planning and teaching purposes.
Dear John, For the ion pumps I've dealt with, the getter assembly is replaced. This is the plates and whatever holds them at the proper separation. I've sent the pump back to the EM manufacturer and gotten a refurbished pump back. I have had only very limited experience with this, however, because the ion pumps on the HVEM were very large with correspondingly large capacity and didn't need replacing for the entire 20+ years I was in Albany, and the pump in our FEG here at Caltech was covered by the service contract. Apparently it developed a whisker on the getter, the symptoms of which were that the vacuum would get bad fairly quickly and shut the FEG off. This happened every few months until the problem was diagnosed. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Jan 30 15:27:07 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0ULR50D032447 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 15:27:06 -0600 6, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 154D61BAF1 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 13:27:04 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id CD64D13E3F 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 13:27:01 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200801302050.m0UKoAts016075-at-ns.microscopy.com} 6, 22 -- References: {200801302050.m0UKoAts016075-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {6EFB292B-2DF8-4A94-BA51-F77952586F50-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] Re: ion pump longevity 6, 22 -- Date: Wed, 30 Jan 2008 13:27:08 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
If the negtive is just slightly denser than "normal" the Canon scanner cannot give a good full tonal scan. The D-Max is insufficient. I have not tried the Epson Larry
vhacopian-at-wellesley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I wish to purchase a flatbed scanner for TEM negatives. I have received } recommendations for the Canon 9950F and the Epson V750-M. The former may } no longer be available, as it is not listed on the Canon web site--only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } ==============================Original Headers============================== } 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 2008 } 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) } 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UDa95N022047 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 -0600 } 2, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) } 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id m0UDa8Jp027778 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 -0500 (EST) } 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) } 2, 18 -- Message-id: {fc.006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} } 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 } 2, 18 -- Subject: TEM - negative scanners } 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) } 2, 18 -- X-FC-SERVER-TZ: 15729388 } 2, 18 -- To: Microscopy-at-Microscopy.Com } 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} } 2, 18 -- MIME-Version: 1.0 } 2, 18 -- Content-Type: text/plain; charset=UTF-8 } 2, 18 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 5, 28 -- From Larry.Ackerman-at-ucsf.edu Wed Jan 30 16:07:40 2008 5, 28 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UM7ewB023069 5, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 16:07:40 -0600 5, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 5, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 5, 28 -- Wed, 30 Jan 2008 14:10:41 -0800 5, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 5, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 5, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Wed, 30 Jan 2008 14:07:33 -0800 5, 28 -- Message-ID: {47A0F525.4040408-at-ucsf.edu} 5, 28 -- Date: Wed, 30 Jan 2008 14:07:33 -0800 5, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 5, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 5, 28 -- Organization: UCSF, NeuroAnatomy 5, 28 -- User-Agent: Thunderbird 2.0.0.9 (Macintosh/20071031) 5, 28 -- MIME-Version: 1.0 5, 28 -- To: Microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] TEM - negative scanners 5, 28 -- References: {200801301347.m0UDlJv5001465-at-ns.microscopy.com} 5, 28 -- In-Reply-To: {200801301347.m0UDlJv5001465-at-ns.microscopy.com} 5, 28 -- X-OriginalArrivalTime: 30 Jan 2008 22:07:33.0413 (UTC) 5, 28 -- FILETIME=[8393C550:01C8638C] 5, 28 -- X-WSS-ID: 6BBE2A6B29O5772273-01-01 5, 28 -- Content-Type: text/plain; 5, 28 -- charset=iso-8859-1; 5, 28 -- format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
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Email: hadden-at-wingate.edu Name: Lee Hadden
Organization: Wingate University
Title-Subject: [Filtered] vacuum problems JEOL JSM5600LV SEM
Question: Our JEOL 5600lv SEM does not attain operating vacuum. It goes through the PRE-EVAC and into the EVAC modes in normal time frame, but remains in EVAC, never going to READY for specimen observation. Any ideas for fixing the problem? Cooling water temp and flow rate OK as are DP temp and oil. The column will hold a vacuum for weeks + even with the SEM off.
It has been a week since my posting. I received 7 replies.
All replies were in favor of smaller dishes with one stating that 96 wells would be even better for his work.
I do not think that this is a large enough group of potential customers for the any company to consider it a valid request to make such a product.
The reason that I did not like the chamber slides was that there was so little flat area when I embedded the cells in a 4 well configuration. I also forgot to use LX-112 (Ladd) in my Epon formula instead of EM-Bed812 (Electron Microscopy Sciences) so the sides of the wells melted a bit making it very hard to get the sides off the polymerized Epon blocks. Perhaps I'll try removing the sides before embedding the next time and use the slide duplicating mold to hold the Epon onto the Permanox slide for curing or get some 2 well chambered slides.
One person, Stephane, asked where I got the 60mm dishes. My supplier is Electron Microscopy Sciences but I believe other suppliers may also carry them.
Thank you for participating. Pat
} For quite some time I have wondered why NUNC did not make a Permanox Petri } Dish smaller than 60 x 15 mm. I have grown to really appreciate the } advantages of using the Permanox over standard Polystyrene dishes for tissue } cultured cells grown for TEM experiments since every cell line that I have } tried adheres well to them, they can withstand chemicals like acetone and } propylene oxide that dissolve the polystyrene and the embedded cells come } away from the Permanox so easily and smoothly. } } With the special cells and reagents that I am now using for TEM, it is a big } waste to grow cells over such a large area when a 35 x 15 mm dish would do } nicely. I do want a dish not a chambered slide. } } I would like to know if there are others (you) } 1. who would switch to a smaller dish if they would be made available. } (this is my pick) } 2. who would like to use both the 60mm and 35mm dishes } 3. who would only use the 60mm dishes } If I get a reasonable response I will contact the company with my results to } back up my request that they consider making the smaller dishes. } } Comments are welcome. } } For the survey, it may be best to answer me "Off-ListServer" so as not to } fill up the emails of other members. I will let the ListServer know the } tally after the replies come in. } } Patricia Stranen Connelly } Biologist, Electron Microscopy } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road South } Bldg. 14E Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-480-6560 } connellyps-at-mail.nih.gov
==============================Original Headers============================== 9, 24 -- From connellyps-at-nhlbi.nih.gov Wed Jan 30 17:07:07 2008 9, 24 -- Received: from NIHCESSMTP3.hub.nih.gov (nihcessmtp3.hub.nih.gov [128.231.90.117]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UN772Y029774 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 17:07:07 -0600 9, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 24 -- Wed, 30 Jan 2008 18:07:02 -0500 9, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.169]) with Microsoft Exchange Server HTTP-DAV ; 9, 24 -- Wed, 30 Jan 2008 23:07:02 +0000 9, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 9, 24 -- Date: Wed, 30 Jan 2008 18:04:26 -0500 9, 24 -- Subject: Re: [Microscopy] Interest in smaller "Permanox" dishes?-Results 9, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 24 -- To: {connellyps-at-nhlbi.nih.gov} 9, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 24 -- Message-ID: {C3C66CAA.1484%connellyps-at-nhlbi.nih.gov} 9, 24 -- Thread-Topic: [Microscopy] Interest in smaller "Permanox" dishes?-Results 9, 24 -- Thread-Index: AchjlHWjtAmjls+HEdyflQANk2Yv1A== 9, 24 -- In-Reply-To: {200801240003.m0O03d9H016406-at-ns.microscopy.com} 9, 24 -- Mime-version: 1.0 9, 24 -- Content-type: text/plain; 9, 24 -- charset="ISO-8859-1" 9, 24 -- X-OriginalArrivalTime: 30 Jan 2008 23:07:02.0666 (UTC) FILETIME=[D3048EA0:01C86394] 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0UN772Y029774 ==============================End of - Headers==============================
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both sandra.gardner-at-xerox.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fvillalovoz-at-deltacollege.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fvillalovoz-at-deltacollege.edu Name: Frank Villalovoz
Question: San Joaquin Delta College is currently seeking candidates for a full-time faculty position in electron microscopy with experience in the biological sciences. The successful candidate should have a strong background in sample preparation for both the TEM and SEM. The position requires individual training of students in microscope operation, ultramicrotomy, critical point drying and all related biological techniques. Teaching responsibilities also include light and electron optics, digital imaging, and other aspects of microscopy as needed. Degree requirements and salary are dependent upon experience.
San Joaquin Delta College in Stockton, California has a two-year training program that has been training students for almost 40 years. It is one of only two programs in the nation at the community college level. We currently have four SEMs, three TEMs, one FIB, a new AFM, and a compliment of biological and materials sample preparation instrumentation. The facility has 18,000 square feet.
Applications and a job description can be obtained at http://www.deltacollege.edu
Application information can be obtained from Jackie Layman, Human Resources, email: jlayman-at-deltacollege.edu (209) 954-5058
For additional information about the laboratory contact:
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Email: ramshes-at-musc.edu Name: Venkat Ramshesh
Organization: Medical University of South Carolina
Title-Subject: [Filtered] Announcement: LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Question: Second Charleston Workshop on
LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Medical University of South Carolina
May 18-23, 2008
The Second Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB) Workshop provides a solid introduction to the concepts and practical applications of light microscopy relevant to modern cell and molecular biology. Students will have opportunities for extensive hands-on experience with state-of-the-art equipment for optical imaging,digital image processing, and fluorescence and confocal/multiphoton microscopy guided by experienced academic and commercial faculty. Lectures and laboratory exercises will include: optics of image formation; microscope alignment; phase contrast and differential interference contrast microscopy; video and digital cameras; contrast enhancement by analog and digital image processing; principles of fluorescence and fluorescence microscopy; ion imaging and fluorescent probes, including green fluorescent protein; fluorescence resonance energy transfer; and laser scanning confocal and multiphoton microscopy. A commercial faculty representing leading microscope manufacturers will make available for student use the latest and most advanced instrumentation for light microscopy, image detection and computerized image analysis. The LMB Workshop is designed for doctoral level scientists, advanced pre-doctoral students and high level technical personnel. No prior experience with microscopy is required. All students will benefit from in-depth interaction with instructors. Students are encouraged to bring their own specimens for analysis.
Tuition: $750.00
Application Deadline: April 1, 2008
Principal Instructors:
John J. Lemasters, M.D., Ph.D., Organizer
P. Darwin Bell, Ph.D.
Prakash Kara, Ph.D.
Margaret Kelly, Ph.D.
Peter Komlosi, Ph.D.
Anna-Liisa Nieminen, Ph.D.
Venkat Ramshesh, Ph.D.
To apply, send a curriculum vita and a brief letter describing your research interests and reasons for enrolling. Because the course is expected to be oversubscribed, applicants should inquire as soon as possible. Please indicate your complete mailing address, telephone/fax number and email address. Full consideration will be given to applications received by April 1, 2008.
For further information or to apply, contact:
Venkat K Ramshesh Medical University of South Carolina Center for Cell Death, Injury and Regeneration and Hollings Cancer Center 280 Calhoun Street, PO Box 250140 Charleston, SC 29425 Telephone (843) 792- 3530, FAX (843) 792-1617 E-mail: ramshes-at-musc.edu
A few cents too : as other have point out, the two main points are :
-at what vacuum levels has the pump worked ? If it works allways at -7 mbar or less, it will be earlier tired, by sputtering it Ti, building a conductive film on it insultors (leakage current, that means than even with a very good vacumm, one has a ion current from microamps to tenth of microamps), memory effects (giving pressure bursts which may shut off the gun, mmmmmmh !), and slow pumping rate. If it has worked at better than -9 mb, it will give good and faithfull service 20 years long. -It needs absolutly to be baked each time it has seen atomspheric pressure, to remove water vapor which limits its pumping cabability, as in each vacuum sytem. A lite bake is processed at 150-250 °C, a much stronger bakeout is done at 300°C. Above 300°C (up to 450 - 700°C) magnets must be removed, but this is more a refurbishment procedure than a maintenance one.
A aspect which is less known, at maybe this is your problem, is that hydrocarbons may paralyse the pumping process. Under the ion current of the pump and the e-beam of a TEM/SEM, one may polymerise them, generating a wide familly of carbon products. These can be a real poison for a ion pump. It's a known issue that one cannot put a ion pump on a vaccum vessel fororganics evaporation (only diff, turbo or cryo). One see always with a mass analyser a strong outgazing of CH4, CO, CO2 and much heavier spices, at the starting or at the shutt off of the HV of the pump, but in these cases much more. We have all seen the same problem with Penning gauges, which are a modified design of the ion pump (measuring without pumping, or pumping without measuring, different design and material choice !).
In the case of a TEM/SEM, one have diff pumps, more or less good traped, rotary vane pumps, more or les good traped too, and in your case, "carbon" samples, which outgas carbon spices. Do you have a LN2 trap near your sample stage ? Is a column vane between the sample stage and the gun ? What is the "normal" vaccum in the gun, and is it measured by a gauge, or the ion pump current ? This last point can give false diagnostic : one think the pump isn't good anymore, and the vacuum level is low, and in fact one have a good vacumm, but a wrong measuremnt, distored by the leakage current of the pump. And one changes the pump ! In that case, the real point to verify is how does the vacumm go down, dynamically, compared with the "normal" state.
I've more experience with ion pumps on clean UHV vessels than on TEMs, but our TEM (Topcon 02B, with Lab6) has the first pump, which begin to be now a bit tired after 15 years of work. Of coarse, we work on materials, not on biological samples.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
nizets2-at-yahoo.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } } We just changed the IGP pump of the gun chamber only 4 years after the installation of our Tecnai G20. This change was advised by a FEI engineer. } We do not even have 400 working hours on this machine and I wondered if it was normal that we already have to change the pump. } We work mainly with ultrathin sections of biological probes embedded in Epon. They are pretty contaminated with carbon, as evidenced by light circles remaining on the sections after even a very short illumination time (I am usually working at 120 kV, LaB6). I am wondering if this carbon contamination, evaporated by the electron beam, is not at least partly responsible for the dirtiness of the pumps (the pump of the column is bigger than the one for the gun, which would explain why it was not so dirty). If this is the case, perhaps a plasma cleaner would not only be a convenience for me but could bring a financial advantage for my boss (I know you see what I mean ;-)). } What would be the cost of a plasma cleaner? } What is your opinion on the question? Do you think that a plasma cleaner would increase the lifetime of the IGPs? } } Best regards, } } Stephane } } } ____________________________________________________________________________________ } Looking for last minute shopping deals? } Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping } } ==============================Original Headers============================== } 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 } 6, 18 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0UF0pGt005637 } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 09:00:53 -0600 } 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 15:00:50 -0000 } 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 18 -- s=s1024; d=yahoo.com; } 6, 18 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:Message-ID; } 6, 18 -- b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9wOTjIMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXfnLKDhyAOWq+S20/9pUAEHFJq8=; } 6, 18 -- X-YMail-OSG: OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3IfmhgAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- } 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Wed, 30 Jan 2008 07:00:50 PST } 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 } 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) } 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 18 -- To: microscopy-at-microscopy.com } 6, 18 -- MIME-Version: 1.0 } 6, 18 -- Content-Type: text/plain; charset=us-ascii } 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Jan 31 03:18:52 2008 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0V9Ioxc010602 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 03:18:51 -0600 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m0V9IjcQ016757 12, 29 -- ; Thu, 31 Jan 2008 10:18:45 +0100 (CET) 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 7CF3C107C7C5; 12, 29 -- Thu, 31 Jan 2008 10:17:03 +0100 (CET) 12, 29 -- Message-ID: {47A19224.20306-at-ipcms.u-strasbg.fr} 12, 29 -- Date: Thu, 31 Jan 2008 10:17:24 +0100 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 12, 29 -- MIME-Version: 1.0 12, 29 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com 12, 29 -- Subject: Re: [Microscopy]ion pump longevity 12, 29 -- References: {200801301556.m0UFucA3015156-at-ns.microscopy.com} 12, 29 -- In-Reply-To: {200801301556.m0UFucA3015156-at-ns.microscopy.com} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-IPCMS-MailScanner: Found to be clean 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.152]); Thu, 31 Jan 2008 10:18:45 +0100 (CET) 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/5619/Wed Jan 30 22:55:02 2008 on mr2.u-strasbg.fr 12, 29 -- X-Virus-Status: Clean 12, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 12, 29 -- version=3.1.8 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr2.u-strasbg.fr ==============================End of - Headers==============================
Avoid the 9950F at all costs (I have one at home, and a few years ago it was the best available but the Twain ScanGear software is absolute rubbish - it can't even scan negatives to A4 and refuses to scan if film isn't in the holders, ie. an unusual size). Plus it has lower resolution than the Epson V750 pro - as does the older Epson 4990 so avoid that one as well.
The present best of the flatbed scanner bunch for large negatives and under £1,000 (assuming you don't want to go to £10,000+) is the £600 Epson V750 Pro. We use it here to scan slide tissue sections as well as TEM negatives - it's scan detail is noticeably better than our Nikon 1x objective and it has far more even illumination (removing the microscope condenser for the 1x objective makes microscope illumination very uneven). Naturally go to a 4x or above objective and the microscope then to wins hands down - but if you want general overviews of a tissue section the Epson V750 is fast and built to scan flat things. Scans of film negatives are generally better in the supplied holders (as the height of the film is right for focus).
In the rush to digital, film scanners are becoming a niche area, so we are lucky there's still a few decent choices at under £1,000 - plus these cheap scanners knock the socks off far more expensive scanners produced in the late 1990s (in terms of image quality/resolution rather than ultimate build quality anyway).
TEM negatives should last 500 years correctly archived so most users scan at around 1,200 dpi for working copies rather than trying to archive images scanned at the full 6,400 dpi resolution where the image size is massive enough to be impractical for PC archiving (actually scanning at 3,200 dpi with the V750 often produces similar detail to 6,400 dpi with most film, but that’s still a very large image size in TIF). Don't throw away the B&W TEM film after scanning, it should last up to 100 times longer than any CD-RW/DVD-RW disk.
So have a look at the V750 Pro - it has better optics and is a more versatile scanner than it's sibling V700 - although other extras like colour targets are more related to colour balance for scanning colour film, but worth having none the less. It scans up to an A4 negative, plus it can scan in A4 reflective mode as well, and the twain Scan software is pretty good. Only downside is the flimsy film holders - some buy new/spare ones* or have their workshop build something suitable if they are scanning film all day.
I can send you a copy of my article on the subject of scanning film [RMS InFocus & Microscopy Now] if you are interested.
Independent V750 review (as a colour film slide scanner) http://www.photo-i.co.uk/index.html http://www.photo-i.co.uk/Reviews/interactive/Epson%20V750/page_1.htm
*e.g. Doug Fishers film holders http://www.betterscanning.com/ http://www.photo-i.co.uk/Reviews/interactive/Epson%204870/DF_holder/MF.htm but may not suite TEM film sizes
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: vhacopian-at-wellesley.edu [mailto:vhacopian-at-wellesley.edu] Sent: 30 January 2008 13:48 To: kjmorris-at-well.ox.ac.uk
I wish to purchase a flatbed scanner for TEM negatives. I have received recommendations for the Canon 9950F and the Epson V750-M. The former may no longer be available, as it is not listed on the Canon web site--only the 8800F is. Any suggestions would be welcome and highly appreciated. Vachik Hacopian
==============================Original Headers============================== 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 2008 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UDa95N022047 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 -0600 2, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id m0UDa8Jp027778 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 -0500 (EST) 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) 2, 18 -- Message-id: {fc.006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 2, 18 -- Subject: TEM - negative scanners 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) 2, 18 -- X-FC-SERVER-TZ: 15729388 2, 18 -- To: Microscopy-at-Microscopy.Com 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} 2, 18 -- MIME-Version: 1.0 2, 18 -- Content-Type: text/plain; charset=UTF-8 2, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 22, 23 -- From kjmorris-at-well.ox.ac.uk Thu Jan 31 04:48:26 2008 22, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VAmQvl026363 22, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jan 2008 04:48:26 -0600 22, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 22, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 22, 23 -- id 1JKWy1-0002S4-Mu 22, 23 -- for Microscopy-at-Microscopy.Com; Thu, 31 Jan 2008 10:48:25 +0000 22, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 22, 23 -- To: {Microscopy-at-Microscopy.Com} 22, 23 -- References: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} 22, 23 -- Subject: RE: [Microscopy] TEM - negative scanners 22, 23 -- Date: Thu, 31 Jan 2008 10:48:38 -0000 22, 23 -- Message-ID: {000901c863f6$d6aac6c0$b22c4381-at-CytoWhizz} 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; 22, 23 -- charset="iso-8859-1" 22, 23 -- X-Mailer: Microsoft Office Outlook 11 22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 22, 23 -- Thread-Index: AchjRrEXp2t+N5l4QUO/MqsR+Ixv+AAqcVsg 22, 23 -- In-Reply-To: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} 22, 23 -- Content-Transfer-Encoding: 8bit 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VAmQvl026363 ==============================End of - Headers==============================
The first step is to discover if the problem is related to poor ultimate vacuum or a bad gauge/controller. I have an independent vacuum gauge installed on my SEM for just this purpose.
Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
-----Original Message----- X-from: hadden-at-wingate.edu [mailto:hadden-at-wingate.edu] Sent: Wednesday, January 30, 2008 5:51 PM To: White, Woody N.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both hadden-at-wingate.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: hadden-at-wingate.edu Name: Lee Hadden
Organization: Wingate University
Title-Subject: [Filtered] vacuum problems JEOL JSM5600LV SEM
Question: Our JEOL 5600lv SEM does not attain operating vacuum. It goes through the PRE-EVAC and into the EVAC modes in normal time frame, but remains in EVAC, never going to READY for specimen observation. Any ideas for fixing the problem? Cooling water temp and flow rate OK as are DP temp and oil. The column will hold a vacuum for weeks + even with the SEM off.
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Wed Jan 30 16:41:28 2008 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UMfO5N004394 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 16:41:26 -0600 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240802c3c6ad86a149-at-[206.69.208.22]} 6, 11 -- Date: Wed, 30 Jan 2008 16:41:22 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: hadden-at-wingate.edu (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: vacuum problems JEOL JSM5600LV SEM 6, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers============================== ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Thu Jan 31 07:32:41 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VDWegm012346 2, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 07:32:40 -0600 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.7568410; 2, 28 -- Thu, 31 Jan 2008 08:32:23 -0500 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Thu, 31 Jan 2008 08:32:23 -0500 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- Subject: RE: [Microscopy] viaWWW: vacuum problems JEOL JSM5600LV SEM 2, 28 -- Date: Thu, 31 Jan 2008 08:32:22 -0500 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87A9B-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200801302251.m0UMpLvu028306-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] viaWWW: vacuum problems JEOL JSM5600LV SEM 2, 28 -- Thread-Index: AchjkrpFG10KD302Rj+uTDfWedbYiwAenvcg 2, 28 -- References: {200801302251.m0UMpLvu028306-at-ns.microscopy.com} 2, 28 -- To: {hadden-at-wingate.edu} , {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 31 Jan 2008 13:32:23.0279 (UTC) FILETIME=[B61D5FF0:01C8640D] 2, 28 -- From: "White, Woody N." {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="us-ascii" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VDWegm012346 ==============================End of - Headers==============================
Our unit (JEOL JSM 5800LV) had an identical problem. It would reach full vacuum, but not switch over to HT READY. This problem came all of a sudden; it didn't drift into the problem with pump times creeping into longer intervals. The fix for our unit was to adjust certain set points.
Our unit is under full service contract, so it was the manufacturer's service tech that repaired our problem. The fix was mentioned in the report... "adjusted TP 1 & 2 set points, TP1 set to 2.754, TP2 set to 1.584". I don't know what that specifically means, nor whether your unit has the same vacuum design. Maybe some of this information can help you.
} Name: Lee Hadden } } Organization: Wingate University } } Title-Subject: [Filtered] vacuum problems JEOL } JSM5600LV SEM } } Question: Our JEOL 5600lv SEM does not attain } operating vacuum. It goes through the PRE-EVAC and } into the EVAC modes in normal time frame, but } remains in EVAC, never going to READY for specimen } observation. Any ideas for fixing the problem? } Cooling water temp and flow rate OK as are DP temp } and oil. The column will hold a vacuum for weeks + } even with the SEM off. }
____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
==============================Original Headers============================== 7, 20 -- From smalinskas-at-yahoo.com Thu Jan 31 07:46:25 2008 7, 20 -- Received: from web34411.mail.mud.yahoo.com (web34411.mail.mud.yahoo.com [66.163.178.160]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0VDkPX9025546 7, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Thu, 31 Jan 2008 07:46:25 -0600 7, 20 -- Received: (qmail 44328 invoked by uid 60001); 31 Jan 2008 13:46:24 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 20 -- b=W7LFUax5kc+I2B9hyx1yi7y2YumVI4BxzQx8trMQaJX8pTTg0FbmH0HnJrI/nQ2dqNNzB/214pFfoUKhWaTHQruLzDl5eHllKyWB6xQFr+u0VDB56Hfq9bg7F1GXsMSRxOi7wPikBUC78ktEGF4kx5JyZAdYmqGAiWblfUjBLZ8=; 7, 20 -- X-YMail-OSG: DBqSMvEVM1kQTMv9VBnBvhOZnpZNJlhGcqcvFGNOo1LWg7ufNSaIg1YiQl4a7ZmSGxoTnpQM6vrc3oSB4lMYfO6jEz0QfaViSvy0dPsPDH_013gvTbg- 7, 20 -- Received: from [141.151.33.213] by web34411.mail.mud.yahoo.com via HTTP; Thu, 31 Jan 2008 05:46:24 PST 7, 20 -- Date: Thu, 31 Jan 2008 05:46:24 -0800 (PST) 7, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: vacuum problems JEOL JSM5600LV SEM 7, 20 -- To: hadden-at-wingate.edu, microscopy-at-ns.microscopy.com 7, 20 -- In-Reply-To: {200801302243.m0UMh72r008475-at-ns.microscopy.com} 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=iso-8859-1 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Message-ID: {732516.40379.qm-at-web34411.mail.mud.yahoo.com} ==============================End of - Headers==============================
I agree about the V750 pro being the current best in that price range. David
On Jan 31, 2008, at 3:52 AM, kjmorris-at-well.ox.ac.uk wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Vachik, } } Avoid the 9950F at all costs (I have one at home, and a few years } ago it was } the best available but the Twain ScanGear software is absolute } rubbish - it } can't even scan negatives to A4 and refuses to scan if film isn't } in the } holders, ie. an unusual size). Plus it has lower resolution than } the Epson } V750 pro - as does the older Epson 4990 so avoid that one as well. } } The present best of the flatbed scanner bunch for large negatives } and under } £1,000 (assuming you don't want to go to £10,000+) is the £600 } Epson V750 } Pro. We use it here to scan slide tissue sections as well as TEM } negatives - } it's scan detail is noticeably better than our Nikon 1x objective } and it has } far more even illumination (removing the microscope condenser for } the 1x } objective makes microscope illumination very uneven). Naturally go } to a 4x } or above objective and the microscope then to wins hands down - but } if you } want general overviews of a tissue section the Epson V750 is fast } and built } to scan flat things. Scans of film negatives are generally better } in the } supplied holders (as the height of the film is right for focus). } } In the rush to digital, film scanners are becoming a niche area, so } we are } lucky there's still a few decent choices at under £1,000 - plus } these cheap } scanners knock the socks off far more expensive scanners produced } in the } late 1990s (in terms of image quality/resolution rather than } ultimate build } quality anyway). } } TEM negatives should last 500 years correctly archived so most } users scan at } around 1,200 dpi for working copies rather than trying to archive } images } scanned at the full 6,400 dpi resolution where the image size is } massive } enough to be impractical for PC archiving (actually scanning at } 3,200 dpi } with the V750 often produces similar detail to 6,400 dpi with most } film, but } that’s still a very large image size in TIF). Don't throw away the } B&W TEM } film after scanning, it should last up to 100 times longer than any } CD-RW/DVD-RW disk. } } So have a look at the V750 Pro - it has better optics and is a more } versatile scanner than it's sibling V700 - although other extras } like colour } targets are more related to colour balance for scanning colour } film, but } worth having none the less. It scans up to an A4 negative, plus it } can scan } in A4 reflective mode as well, and the twain Scan software is } pretty good. } Only downside is the flimsy film holders - some buy new/spare ones* } or have } their workshop build something suitable if they are scanning film } all day. } } I can send you a copy of my article on the subject of scanning film } [RMS } InFocus & Microscopy Now] if you are interested. } } Independent V750 review (as a colour film slide scanner) } http://www.photo-i.co.uk/index.html } http://www.photo-i.co.uk/Reviews/interactive/Epson%20V750/page_1.htm } } *e.g. Doug Fishers film holders } http://www.betterscanning.com/ } http://www.photo-i.co.uk/Reviews/interactive/Epson%204870/DF_holder/ } MF.htm } but may not suite TEM film sizes } } Keith } } ---------------------------------------------------------------------- } ---- } Dr keith J. Morris } Molecular Cytogenetics and Microscopy Core } Laboratory 00/069 and 00/070 } The Wellcome Trust Centre for Human Genetics } Roosevelt Drive } Oxford } OX3 7BN } United Kingdom } Telephone: +44 (0)1865 287568 } Email: kjmorris-at-well.ox.ac.uk } Web-pages: http://www.well.ox.ac.uk/cytogenetics/ } } } } -----Original Message----- } X-from: vhacopian-at-wellesley.edu [mailto:vhacopian-at-wellesley.edu] } Sent: 30 January 2008 13:48 } To: kjmorris-at-well.ox.ac.uk } Subject: [Microscopy] TEM - negative scanners } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I wish to purchase a flatbed scanner for TEM negatives. I have } received } recommendations for the Canon 9950F and the Epson V750-M. The } former may } no longer be available, as it is not listed on the Canon web site-- } only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } ==============================Original } Headers============================== } 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 } 2008 } 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu } [149.130.13.51]) } 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m0UDa95N022047 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 } -0600 } 2, 18 -- Received: from firstclass.wellesley.edu } (firstclass.wellesley.edu } [149.130.13.40]) } 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id } m0UDa8Jp027778 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 } -0500 (EST) } 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) } 2, 18 -- Message-id: } {fc. } 006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} } 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 } 2, 18 -- Subject: TEM - negative scanners } 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) } 2, 18 -- X-FC-SERVER-TZ: 15729388 } 2, 18 -- To: Microscopy-at-Microscopy.Com } 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} } 2, 18 -- MIME-Version: 1.0 } 2, 18 -- Content-Type: text/plain; charset=UTF-8 } 2, 18 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 22, 23 -- From kjmorris-at-well.ox.ac.uk Thu Jan 31 04:48:26 2008 } 22, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m0VAmQvl026363 } 22, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jan 2008 } 04:48:26 -0600 } 22, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 22, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 22, 23 -- id 1JKWy1-0002S4-Mu } 22, 23 -- for Microscopy-at-Microscopy.Com; Thu, 31 Jan 2008 10:48:25 } +0000 } 22, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 22, 23 -- To: {Microscopy-at-Microscopy.Com} } 22, 23 -- References: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} } 22, 23 -- Subject: RE: [Microscopy] TEM - negative scanners } 22, 23 -- Date: Thu, 31 Jan 2008 10:48:38 -0000 } 22, 23 -- Message-ID: {000901c863f6$d6aac6c0$b22c4381-at-CytoWhizz} } 22, 23 -- MIME-Version: 1.0 } 22, 23 -- Content-Type: text/plain; } 22, 23 -- charset="iso-8859-1" } 22, 23 -- X-Mailer: Microsoft Office Outlook 11 } 22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 22, 23 -- Thread-Index: AchjRrEXp2t+N5l4QUO/MqsR+Ixv+AAqcVsg } 22, 23 -- In-Reply-To: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} } 22, 23 -- Content-Transfer-Encoding: 8bit } 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m0VAmQvl026363 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 23 -- From Elliott-at-arizona.edu Thu Jan 31 10:06:28 2008 6, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VG6Rrq012449 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 10:06:27 -0600 6, 23 -- Received: from gandalfs_amavis (amavis4.email.arizona.edu [10.0.0.207]) 6, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 10A6F3DF99B 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 09:06:27 -0700 (MST) 6, 23 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 6, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4F2AF3DF994 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 09:06:23 -0700 (MST) 6, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 23 -- In-Reply-To: {200801311052.m0VAqILG031107-at-ns.microscopy.com} 6, 23 -- References: {200801311052.m0VAqILG031107-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 6, 23 -- Message-Id: {05B35F5A-AA6E-442A-9337-89C5833EE59B-at-arizona.edu} 6, 23 -- From: David Elliott {Elliott-at-arizona.edu} 6, 23 -- Subject: Re: [Microscopy] RE: TEM - negative scanners 6, 23 -- Date: Thu, 31 Jan 2008 09:06:22 -0700 6, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 23 -- X-Mailer: Apple Mail (2.753) 6, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VG6Rrq012449 ==============================End of - Headers==============================
I agree with Jessica that you should use cryomicrotomy and collect dry sections. This is a challenging technique but with practice you will find it to be valuable. Microtome vendors and other training facilities offer workshops on the cryomicrotomy.
In our lab we use a 'plastic chuck' for sectioning films at ambient and low temperature. The chuck consists of a piece of plastic with approximate dimensions of 4 mm x 7 mm x 3 mm (width x length x thickness). These dimensions should be set to your personal preference and to fit your sample and microtome chuck. Use a stiff plastic such as high density polyethylene or polypropylene that will hold the sample tight but is not rigid or inflexible. Using a sharp razor blade, bisect the 3 mm thickness, cutting through nearly the full length of the plastic piece and leaving a couple of millimeters of at the other end of the piece to hold the arms of the chuck together. The end results is a Y-shaped piece of plastic (see diagram below). To use the chuck, slide the film between the arms of the plastic chuck leaving less than 1 mm exposed; the more film is exposed, the more likely it is to move side-to-side during microtomy. Listers may contact me off-line for a drawing of the chuck.
I recommend that you do not embed films in epoxy if at all possible. The embedding medium, whatever it might be, only contributes to the difficulty of cutting. The problem is that the film has several layers, each with different cutting properties at low temperature; the problem is often even worse at ambient temperature. Adding the embedding medium contributes another medium that most probably has very different cutting properties than any material in the film.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
cervantes-at-bend res.com To gary.m.brown-at-exxonmobil.com 01/30/08 07:40 cc PM Subject [Microscopy] RE: viaWWW: 2 questions Please respond concerning microtomy of thin films to cervantes-at-bend res.com
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Sandra -
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
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Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
As I have suggested in the past, a digital camera can be used to copy TEM negatives (or any larger format film). I put my negatives, emulsion side up, on a light box, mask off the unwanted light with heavy black paper or cardboard, and photograph the negative with an older (4 years or so) Canon G3 camera mounted on a copy stand to insure stability. Most better digital cameras will focus close enough to fill the frame with the negative (3.25 x 4 inches). I download the images to Photoshop and invert to a positive. This is much faster than using a flatbed scanner. The quality is fine for most applications. If you must have higher quality for publication or making mural size prints, take the negatives to a shop that has a drum scanner.
Geoff vhacopian-at-wellesley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I wish to purchase a flatbed scanner for TEM negatives. I have received } recommendations for the Canon 9950F and the Epson V750-M. The former may } no longer be available, as it is not listed on the Canon web site--only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } } -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 5, 28 -- From mcauliff-at-umdnj.edu Thu Jan 31 10:15:48 2008 5, 28 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VGFlbo027927 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 10:15:48 -0600 5, 28 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 5, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 41CDB4BF14 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:46 -0500 (EST) 5, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 5, 28 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 778864BE91 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:45 -0500 (EST) 5, 28 -- Received: from ([10.32.15.171]) 5, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.140113879; 5, 28 -- Thu, 31 Jan 2008 11:15:20 -0500 5, 28 -- MIME-version: 1.0 5, 28 -- Content-transfer-encoding: 7BIT 5, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 5, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 5, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0JVI00M3DNTJ8530-at-umduwc02.umdnj.edu} for 5, 28 -- microscopy-at-microscopy.com; Thu, 31 Jan 2008 11:15:20 -0500 (EST) 5, 28 -- Message-id: {47A1F430.8030401-at-umdnj.edu} 5, 28 -- Date: Thu, 31 Jan 2008 11:15:44 -0500 5, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 5, 28 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 5, 28 -- To: vhacopian-at-wellesley.edu, microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] TEM - negative scanners 5, 28 -- References: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} 5, 28 -- In-reply-to: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} ==============================End of - Headers==============================
I doubt if microwave exposure (cooking) will soften embalmed tissue. You might try soaking the organ in water (tap water) for a week, changing the water every day. There is good evidence that formalin-fixed tissue can be "unfixed", at least to some extent, by prolonged washing in water. Of course, it will depend on the composition of the embalming fluid, the length of the exposure, etc.
Geoff
tbargar-at-unmc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I've got a surgeon who is working with human livers that are very hard from } the embalming process and he had heard that microwaving tissue may soften } up the tissue and make it easier to cut. I'm going to let him use my } Biowave and try different wattages. I have never heard of this effect } before. Has anybody out there heard of or actually know if hardened fixed } tissue can be softened by microwaves. Thanks, all help is appreciated. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original Headers============================== } 4, 20 -- From tbargar-at-unmc.edu Tue Jan 29 09:29:00 2008 } 4, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) } 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TFT028004622 } 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 } 4, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) } 4, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 2FE894C084 } 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 (CST) } 4, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) } 4, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id C51AF4C06E } 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:28:58 -0600 (CST) } 4, 20 -- Subject: Can hardened fixed tissue be "softened" by Microwave treatment? } 4, 20 -- To: Microscopy-at-MSA.Microscopy.com } 4, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 } 4, 20 -- Message-ID: {OF0B63F2D6.945FCCD8-ON862573DF.005434C9-862573DF.00550C8B-at-unmc.edu} } 4, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} } 4, 20 -- Date: Tue, 29 Jan 2008 09:28:57 -0600 } 4, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/29/2008 09:28:58 } 4, 20 -- AM } 4, 20 -- MIME-Version: 1.0 } 4, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 29 -- From mcauliff-at-umdnj.edu Thu Jan 31 10:21:58 2008 9, 29 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VGLuWl014928 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 10:21:57 -0600 9, 29 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 9, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 0E6224BF07 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:21:56 -0500 (EST) 9, 29 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 9, 29 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 242A54BE8B 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:21:55 -0500 (EST) 9, 29 -- Received: from ([10.32.15.171]) 9, 29 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.140114859; 9, 29 -- Thu, 31 Jan 2008 11:21:30 -0500 9, 29 -- MIME-version: 1.0 9, 29 -- Content-transfer-encoding: 7BIT 9, 29 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Received: from [127.0.0.1] ([10.32.15.102]) 9, 29 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 9, 29 -- Oct 12 2007; 32bit)) with ESMTP id {0JVI00MMNO3U8530-at-umduwc02.umdnj.edu} for 9, 29 -- microscopy-at-microscopy.com; Thu, 31 Jan 2008 11:21:30 -0500 (EST) 9, 29 -- Message-id: {47A1F5A2.4070709-at-umdnj.edu} 9, 29 -- Date: Thu, 31 Jan 2008 11:21:54 -0500 9, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 29 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 9, 29 -- To: tbargar-at-unmc.edu, microscopy-at-microscopy.com 9, 29 -- Subject: Re: [Microscopy] Can hardened fixed tissue be "softened" by Microwave 9, 29 -- treatment? 9, 29 -- References: {200801291530.m0TFUBG4007543-at-ns.microscopy.com} 9, 29 -- In-reply-to: {200801291530.m0TFUBG4007543-at-ns.microscopy.com} ==============================End of - Headers==============================
My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., IMO.
If you think you have carbon or hydrocarbon issues, have your serviceman check the long column tubing liner just under the anode cap for a black coating of residue that builds up on the liner's inner wall. There should be a tool in the factory tool kit to remove this tubing liner. Look at the amount of the deposit and the color. Ask the serviceman if that amount of deposit seems normal or excessive. I would have a supply of 6-8 inch long wooden "Q-tip swabs" and Pol polish handy for him.
HTH,
Paul
At 09:01 AM 1/30/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 16 -- From beaurega-at-westol.com Thu Jan 31 11:24:28 2008 6, 16 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0VHOSca002606 6, 16 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:24:28 -0600 6, 16 -- Received: (qmail 16520 invoked by uid 89); 31 Jan 2008 17:24:26 -0000 6, 16 -- Received: from pitts-69-72-117-2.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.2) 6, 16 -- by mail.winbeam.com with SMTP; 31 Jan 2008 17:24:26 -0000 6, 16 -- Message-Id: {3.0.6.32.20080131122424.007b6420-at-pop3.norton.antivirus} 6, 16 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified) 6, 16 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 6, 16 -- Date: Thu, 31 Jan 2008 12:24:24 -0500 6, 16 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com 6, 16 -- From: Beaurega {beaurega-at-westol.com} 6, 16 -- Subject: Re: [Microscopy] ion pump longevity 6, 16 -- Mime-Version: 1.0 6, 16 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
A modern flatbed film scanner (e.g. the Epson V750) can scan a negative into PhotoShop within a few minutes at 1,200 dpi - and there's no photo download from camera to PC involved afterwards. At 6,400 dpi things do take a bit longer, but that's well beyond the resolution of just about any camera prime lens and indeed most colour slide film for that matter. If you compare a camera macro image of a negative/slide to an Epson V750 film scan, it's no contest - the Epson wins hands down.
TEM film has a higher theoretical resolution than 6,400 dpi but the resulting TIF image file size from a slow 6,400 dpi V750 scan would be highly prone to corruption if archived digitally on a PC anyway. You can easily use 3,200 dpi to 'enlarge' selected areas of the negative though. Plus we don't need Digital ICE scratch removal, which is the main process that markedly slows down film scan speeds [but it can introduce artifacts and affect detail, and anyway it isn't available for B&W film].
Besides, any process that passes an image back through another set of optics will always further degrade that image. You should use a 8-10x inspection magnifier and a light box to compare the scanned image with the original film - the difference in resolution is small but clearly visible. Whatever scanner is used, some detail is lost (just as detail was lost when the original view was captured). Plus you really need to get the film/specimen height and flatness right on a flatbed scanner, normally this means using the supplied film holder (if the films at the wrong height from the platen the scan image can be out of focus).
You do always get an apparent large reduction in shadow detail with a pro-sumer V750 type flat bed scanner with colour slide film (shadows look far too black), but the detail is actually still there and you can bring it right back to the correct level of brightness using Photoshop's 'shadow/highlight' command. This quickly restores the shadow detail to the scanned image. You can use PhotoShop's 'unsharp mask' [not much] to add back a bit of sharpness at 100% mag as well. Always look at the original film using a light box and 8-10x magnifier to ensure you get this post-scan processing just right if that’s important to you.
The Epson 750 Pro produces image scans of comparable (if not indistinguishable) quality to those of an £10k Hasselblad Imacon Flextight 848 semi-drum scanner - granted at far lower scan speeds. Besides 100 TEM film scans using a Hasselblad in your Reprographics department would cost you more than an Epson V750 pro anyway.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: mcauliff-at-umdnj.edu [mailto:mcauliff-at-umdnj.edu] Sent: 31 January 2008 16:23 To: kjmorris-at-well.ox.ac.uk
Greetings all:
As I have suggested in the past, a digital camera can be used to copy TEM negatives (or any larger format film). I put my negatives, emulsion side up, on a light box, mask off the unwanted light with heavy black paper or cardboard, and photograph the negative with an older (4 years or so) Canon G3 camera mounted on a copy stand to insure stability. Most better digital cameras will focus close enough to fill the frame with the negative (3.25 x 4 inches). I download the images to Photoshop and invert to a positive. This is much faster than using a flatbed scanner. The quality is fine for most applications. If you must have higher quality for publication or making mural size prints, take the negatives to a shop that has a drum scanner.
Geoff vhacopian-at-wellesley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I wish to purchase a flatbed scanner for TEM negatives. I have received } recommendations for the Canon 9950F and the Epson V750-M. The former may } no longer be available, as it is not listed on the Canon web site--only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } } -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 5, 28 -- From mcauliff-at-umdnj.edu Thu Jan 31 10:15:48 2008 5, 28 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VGFlbo027927 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 10:15:48 -0600 5, 28 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 5, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 41CDB4BF14 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:46 -0500 (EST) 5, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 5, 28 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 778864BE91 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:45 -0500 (EST) 5, 28 -- Received: from ([10.32.15.171]) 5, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.140113879; 5, 28 -- Thu, 31 Jan 2008 11:15:20 -0500 5, 28 -- MIME-version: 1.0 5, 28 -- Content-transfer-encoding: 7BIT 5, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 5, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 5, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0JVI00M3DNTJ8530-at-umduwc02.umdnj.edu} for 5, 28 -- microscopy-at-microscopy.com; Thu, 31 Jan 2008 11:15:20 -0500 (EST) 5, 28 -- Message-id: {47A1F430.8030401-at-umdnj.edu} 5, 28 -- Date: Thu, 31 Jan 2008 11:15:44 -0500 5, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 5, 28 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 5, 28 -- To: vhacopian-at-wellesley.edu, microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] TEM - negative scanners 5, 28 -- References: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} 5, 28 -- In-reply-to: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 23 -- From kjmorris-at-well.ox.ac.uk Thu Jan 31 11:33:56 2008 21, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 21, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VHXuHx016349 21, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jan 2008 11:33:56 -0600 21, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 21, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 21, 23 -- id 1JKdIR-0006Do-Qk 21, 23 -- for Microscopy-at-Microscopy.Com; Thu, 31 Jan 2008 17:33:55 +0000 21, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 21, 23 -- To: {Microscopy-at-Microscopy.Com} 21, 23 -- References: {200801311623.m0VGN5t8018582-at-ns.microscopy.com} 21, 23 -- Subject: RE: [Microscopy] Re: TEM - negative scanners 21, 23 -- Date: Thu, 31 Jan 2008 17:34:08 -0000 21, 23 -- Message-ID: {000901c8642f$7c139dc0$b22c4381-at-CytoWhizz} 21, 23 -- MIME-Version: 1.0 21, 23 -- Content-Type: text/plain; 21, 23 -- charset="iso-8859-1" 21, 23 -- X-Mailer: Microsoft Office Outlook 11 21, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 21, 23 -- Thread-Index: AchkJY/ZmC9QjEHURfuwINJhalAmEgAATiMg 21, 23 -- In-Reply-To: {200801311623.m0VGN5t8018582-at-ns.microscopy.com} 21, 23 -- Content-Transfer-Encoding: 8bit 21, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VHXuHx016349 ==============================End of - Headers==============================
I suspect that the Canon and Epson scanners will be more readily available for negatives but it is still worth checking the specification and prices of the Microtek i800 (semi pro) and i900 (pro). They both have pretty good resolution and an excellect Dmax as well as using a separate glassless drawer for negatives up to about 12 x 10 inches.
eg i800 review: "What's affordable about $400 list? How about a Dmax of 4.0, 48-bit color and 9600x4800 dpi optical resolution on a legal-sized scanning bed with your choice of High-Speed USB 2.0 or FireWire ports?" from http://www.imaging-resource.com/SCAN/MI8/MI8.HTM
The i900 is about twice the price but has an improved Dmax of 4.2.
I personally use the Microtek Scanmaker 8700 which still serves me well but doesn't have the resolution or high Dmax of the two above.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: vhacopian-at-wellesley.edu
In response to Tom Bargar's question,
I am not aware that microwaves can soften embalmed human liver tissues. On the other hand, plant biologists often soak plant tissues in glycerol to soften them for sectioning.
Check out the following article:
John C. Guenther and Frances Trail. 2005. The development and differentiation of Gibberella zeae (anamorph: Fusarium graminearum) during colonization of wheat. Mycologia, 97(1), 2005, pp. 229-237.
In this study, the authors did the following:
After a 24 h fixation, samples were placed in a solution of glycerol and ethanol (1:1) for an additional 24 h to soften tissues for sectioning.
They then further dehydrated the specimens in alcohol, embedded (in paraffin, in this case) and sectioned as usual. You might give it a try. Certainly, use the web to search for the use of glycerine as a softener since I know it is used successfully.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
All: is there anyone in the north Texas area that has an SEM that will take a 300mm (12-inch) wafer? I need to image the surface of a fully processed, bumped wafer without coating it or breaking it.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 3, 21 -- From r-holdford-at-ti.com Thu Jan 31 16:15:53 2008 3, 21 -- Received: from soda.ext.ti.com (soda.ext.ti.com [198.47.26.145]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VMFrei018228 3, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 16:15:53 -0600 3, 21 -- Received: from dlep32.itg.ti.com ([157.170.170.70]) 3, 21 -- by soda.ext.ti.com (8.13.7/8.13.7) with ESMTP id m0VMFllG014552 3, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 16:15:52 -0600 3, 21 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 3, 21 -- by dlep32.itg.ti.com (8.13.7/8.13.7) with ESMTP id m0VMFlia025841 3, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 16:15:47 -0600 (CST) 3, 21 -- Message-ID: {47A24892.2020405-at-ti.com} 3, 21 -- Date: Thu, 31 Jan 2008 16:15:46 -0600 3, 21 -- From: Becky Holdford {r-holdford-at-ti.com} 3, 21 -- Organization: SC Packaging Development -- FA Development 3, 21 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 3, 21 -- MIME-Version: 1.0 3, 21 -- To: MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 3, 21 -- Subject: 300mm wafer-capable SEM in North Texas? 3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Microbeam Analysis Society is sponsoring a Technical Conference on EBSD May 20-22 at the University of Wisconsin-Madison. The 3 day workshop will start with a full day tutorial session for newcomers to the technique. Student participation is being encouraged with 10 $500 scholarships. For more information and online registration materials, go to www. microbeamanalysis.org. Space is limited so early registration is strongly recommended.
John Fournelle johnf-at-geology.wisc.edu
==============================Original Headers============================== 2, 24 -- From johnf-at-geology.wisc.edu Thu Jan 31 18:09:44 2008 2, 24 -- Received: from mail.geology.wisc.edu (mail.geology.wisc.edu [144.92.206.10]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1109iK2003552 2, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 18:09:44 -0600 2, 24 -- Received: from localhost (mail.geology.wisc.edu [127.0.0.1]) 2, 24 -- by localhost (Postfix) with ESMTP id 095DD5C812D 2, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 18:09:44 -0600 (CST) 2, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu 2, 24 -- Received: from mail.geology.wisc.edu ([127.0.0.1]) 2, 24 -- by localhost (mail.geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 2, 24 -- with ESMTP id s1dDmOaTrh0F for {Microscopy-at-MSA.Microscopy.Com} ; 2, 24 -- Thu, 31 Jan 2008 18:09:37 -0600 (CST) 2, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 2, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 2, 24 -- (No client certificate requested) 2, 24 -- by mail.geology.wisc.edu (Postfix) with ESMTP id 6DBC15C8117 2, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 18:09:37 -0600 (CST) 2, 24 -- Mime-Version: 1.0 2, 24 -- Message-Id: {p0623091ec3c812cab2e5-at-[144.92.206.57]} 2, 24 -- Date: Thu, 31 Jan 2008 18:09:35 -0600 2, 24 -- To: Microscopy-at-MSA.Microscopy.Com 2, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 2, 24 -- Subject: EBSD workshop May 20-22 2, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikroskop-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mikroskop-at-gmail.com Name: Jack Hietpas
Organization: Syracuse University
Title-Subject: [Filtered] magnification in CL images
Question: Hello: We have a JEOL 8600 Superprobe with what has been described as the "Poor man's" cathodoluminescence (CL) detector, which is simply a PMT inserted in the optical path of the probe's integrated light microscope. I have a very basic question concerning the system: where does the magnification of CL image come from? In addition why is the field of view the exact same as that in BSE or SEI? I find it hard to believe (perhaps incorrectly) that the magnification comes from the light microscope: the magnification is continuous as opposed to the fixed mag (focal length) of the light microscope and the mag range of the CL images is beyond the useful mag of the microscope. What am I missing?
I don't know your exact setup, but my guess is that the signal from the PMT is synchronized with the beam scanning on the sample. For each position on the sample (pixel) the magnitude of the PMT is recorded as the intensity on the image. Just like a SE or BSE images.
Hendrix
On Jan 31, 2008 7:40 PM, {mikroskop-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mikroskop-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mikroskop-at-gmail.com } Name: Jack Hietpas } } Organization: Syracuse University } } Title-Subject: [Filtered] magnification in CL images } } Question: Hello: We have a JEOL 8600 Superprobe with what has been described as the "Poor man's" cathodoluminescence (CL) detector, which is simply a PMT inserted in the optical path of the probe's integrated light microscope. I have a very basic question concerning the system: where does the magnification of CL image come from? In addition why is the field of view the exact same as that in BSE or SEI? I find it hard to believe (perhaps incorrectly) that the magnification comes from the light microscope: the magnification is continuous as opposed to the fixed mag (focal length) of the light microscope and the mag range of the CL images is beyond the useful mag of the microscope. What am I missing? } } -jh } } } Login Host: 69.205.65.34 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Thu Jan 31 18:34:01 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m110XxSQ016698 } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 18:34:00 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240803c3c81966eb8e-at-[206.69.208.22]} } 8, 11 -- Date: Thu, 31 Jan 2008 18:33:58 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: mikroskop-at-gmail.com (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: magnification in CL images } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 30 -- From drix00-at-gmail.com Thu Jan 31 19:11:44 2008 4, 30 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.249]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m111BhZc030147 4, 30 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 19:11:44 -0600 4, 30 -- Received: by an-out-0708.google.com with SMTP id b33so224852ana.21 4, 30 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 17:11:43 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 30 -- bh=QmtmXaAwdd+cpAgKgBkc/W1R96IpgB3pQeYIOiuK0SM=; 4, 30 -- b=wjMK8fSGAuExBtV3TWnZiE+4QPtelxnQgJcLrbaTOHo14XvxXah3z52eMxBlVNMqYy+nb5xokNxQjsZP3lovq8Keyt+8QXnID9Nn4SkIizOuteiPFpg99h1gJOYPlji7iO8Kfg7J3B0tW7GgehSPjtj2hy+hOEbU+Br4A9Zf2fk= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 30 -- b=suK0R7qfXBlDj+kd5Wob9vjDoeA3lk70JL/FUAKSQEPupeM9JcaRXLhGAhIHwiX8BjR6K9KxfF5rJLCNNqqiqTyrBjCB2gWgbaEGvF2dKhCxbbzm4gAs3QRY6tf7oEozYBgnQ93KuarOfo+c6cgw5DB+EjN+7KGyPz2+H55Us5Q= 4, 30 -- Received: by 10.101.70.5 with SMTP id x5mr5961529ank.59.1201828303554; 4, 30 -- Thu, 31 Jan 2008 17:11:43 -0800 (PST) 4, 30 -- Received: by 10.100.91.18 with HTTP; Thu, 31 Jan 2008 17:11:43 -0800 (PST) 4, 30 -- Message-ID: {a779eeae0801311711y4f33dfa4hbf10abfc046f80e8-at-mail.gmail.com} 4, 30 -- Date: Thu, 31 Jan 2008 20:11:43 -0500 4, 30 -- From: drix {drix00-at-gmail.com} 4, 30 -- To: mikroskop-at-gmail.com 4, 30 -- Subject: Re: [Microscopy] viaWWW: magnification in CL images 4, 30 -- Cc: microscopy-at-microscopy.com 4, 30 -- In-Reply-To: {200802010040.m110eUxR025164-at-ns.microscopy.com} 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- Content-Disposition: inline 4, 30 -- References: {200802010040.m110eUxR025164-at-ns.microscopy.com} ==============================End of - Headers==============================
I have someone who wants to look at "changes" at the EM level caused by experimental drugs, in guinea pig eyes. Hopefully he'll come up with a more specific aim, but in the meantime does anyone have experience fixing guinea pigs? I'll get him to perfuse the animals, but would like to choose the best fixative. Any suggestions?
Thanks
Diana
Diana van Driel
Discipline of Ophthalmology Sydney University GPO Box 4337 Sydney, NSW AUSTRALIA 2001
The Microtek i800 scanner optical resolution is quoted at 4,800 dpi to the Epsons V700/V750s 6,400 dpi - so it's probably on a par with the older 4,800 dpi Epsons and Canons - both of which were perfectly fine for TEM film scans at 1,200 dpi anyway (other than Canon's Scangear being too stupid to notice any film was on the platter).
Dmax is less important with B&W film (and this includes TEM) but is essential resolving shadows with colour slides. Most modern scanners will have a high enough DMax for B&W TEM film. The Hasselblad flextight has a superb DMax and shadow detail in a colour slide was resolved very well, but no better than the cheaper Epsons after post-processsing (the colour slide film itself was the limiting factor really). The better resolution of the 8,000 dpi Hasselblad Flextight 848 drum scanner just picked out the detail of the colour film grain better, and surprisingly didn't really get any extra information from B&W TEM film (but I couldn't get to this scanner and tweak it optimally for the TEM film - it was operated by the universities Reprographics staff at £10 a scan.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: 31 January 2008 17:46 To: kjmorris-at-well.ox.ac.uk
Vachik
I suspect that the Canon and Epson scanners will be more readily available for negatives but it is still worth checking the specification and prices of the Microtek i800 (semi pro) and i900 (pro). They both have pretty good resolution and an excellect Dmax as well as using a separate glassless drawer for negatives up to about 12 x 10 inches.
eg i800 review: "What's affordable about $400 list? How about a Dmax of 4.0, 48-bit color and 9600x4800 dpi optical resolution on a legal-sized scanning bed with your choice of High-Speed USB 2.0 or FireWire ports?" from http://www.imaging-resource.com/SCAN/MI8/MI8.HTM
The i900 is about twice the price but has an improved Dmax of 4.2.
I personally use the Microtek Scanmaker 8700 which still serves me well but doesn't have the resolution or high Dmax of the two above.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: vhacopian-at-wellesley.edu
Diana,
Years ago when we were perfusing mice and hamsters, we used a modified Krebs' Perfusion Buffer to flush the circulatory system, then fixed with a solution of 1% glut, 3% formaldehyde in PBS. Recipe for 100 mL Krebs is as follows (added in the order in which they appear):
0.17 g dextrose 0.02 g NaNO2 0.028 g heparin 2.0 mL 1M HEPES 1.0 g BSA 0.16 mL CaCl2 solution (0.147 g/mL) pH 7.4
It takes about 40 mL to perfuse a mouse, so scale up accordingly.
Daryl Meyer
----- Original Message ----- X-from: dianavd-at-eye.usyd.edu.au
There are three little tricks for extending the life of ion pumps that have not been mentioned yet that I thought I could contribute based on years of experience with JEOL and Topcon TEM's:
1) The hammer. Yes, just smack the bajeezus out of the ion pump body (not the magnet) with a hammer. The shock knocks most of the whiskers and flakes loose. Do it with the emitter cold, because it will shock the whole TEM. Put emphasis on a large number of mild whacks in every direction of the pump housing. Keep the pump HV on, reading pump current and you will see lots of little spikes as the whiskers and flakes get dislodged. Wear hearing protection because you will need to do this for about 20 minutes. When you stop seeing any spike in ion current with hammer whacks, you are done.
2) Vent the system, pump it down, and restart the ion pump prematurely with over current protection turned off. The pump should get hot. That's basically a poor man's bake. It works.
3) Hi-pot. This is a technique where the service engineer brings in a high current ~10KV power supply and connects it to the pump for about an hour. The high voltage and high current blast all the whiskers and flakes quite nicely. However, if you have never done this yourself, get the service engineer to do it and be paranoid because it is VERY DANGEROUS! If the standard power supply for ion pumps wasn't dangerous enough, this is one that should treated with the greatest caution.
John Mardinly, Intel
This is not necessarily an opinion of Intel Corporation
-----Original Message----- X-from: beaurega-at-westol.com [mailto:beaurega-at-westol.com] Sent: Thursday, January 31, 2008 9:25 AM To: Mardinly, John
Hi,
My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., IMO.
If you think you have carbon or hydrocarbon issues, have your serviceman check the long column tubing liner just under the anode cap for a black coating of residue that builds up on the liner's inner wall. There should be a tool in the factory tool kit to remove this tubing liner. Look at the amount of the deposit and the color. Ask the serviceman if that amount of deposit seems normal or excessive. I would have a supply of 6-8 inch long wooden "Q-tip swabs" and Pol polish handy for him.
HTH,
Paul
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==============================Original Headers============================== 19, 32 -- From john.mardinly-at-intel.com Fri Feb 1 14:48:11 2008 19, 32 -- Received: from mga11.intel.com (mga11.intel.com [192.55.52.93]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m11KmAUv022712 19, 32 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Feb 2008 14:48:11 -0600 19, 32 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 19, 32 -- by fmsmga102.fm.intel.com with ESMTP; 01 Feb 2008 12:45:49 -0800 19, 32 -- X-ExtLoop1: 1 19, 32 -- X-IronPort-AV: E=Sophos;i="4.25,292,1199692800"; 19, 32 -- d="scan'208";a="511891848" 19, 32 -- Received: from orsmsx334.jf.intel.com ([10.22.226.45]) 19, 32 -- by fmsmga001.fm.intel.com with ESMTP; 01 Feb 2008 12:48:10 -0800 19, 32 -- Received: from orsmsx423.amr.corp.intel.com ([10.22.226.104]) by orsmsx334.jf.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 32 -- Fri, 1 Feb 2008 12:47:57 -0800 19, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 32 -- Content-class: urn:content-classes:message 19, 32 -- MIME-Version: 1.0 19, 32 -- Content-Type: text/plain; 19, 32 -- charset="us-ascii" 19, 32 -- Subject: RE: [Microscopy] Re: ion pump longevity 19, 32 -- Date: Fri, 1 Feb 2008 12:47:56 -0800 19, 32 -- Message-ID: {36C438BD1408B1499A19BA5730AAFC63BB8886-at-orsmsx423.amr.corp.intel.com} 19, 32 -- X-MS-Has-Attach: 19, 32 -- X-MS-TNEF-Correlator: 19, 32 -- Thread-Topic: [Microscopy] Re: ion pump longevity 19, 32 -- Thread-Index: AchkLze/C1E+7SDCT8eefxWk4GsNmwAxle8AAAeDmAA= 19, 32 -- References: {200801311724.m0VHObHi002733-at-ns.microscopy.com} 19, 32 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 19, 32 -- To: {beaurega-at-westol.com} 19, 32 -- Cc: {Microscopy-at-msa.microscopy.com} 19, 32 -- X-OriginalArrivalTime: 01 Feb 2008 20:47:57.0722 (UTC) FILETIME=[B9DEA7A0:01C86513] 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m11KmAUv022712 ==============================End of - Headers==============================
As usual Gary Brown is dead on with his comments. I agree that embedment is generally best avoided in these multilayer applications. We have seen swelling of the surface layer due to epoxy interaction as well, surprising some real polymer gurus around here. His specimen holder sounds elegant - I think I'll make one. We've taken a more down-and-dirty, and semi-disposable, approach here that may be useful in some of your applications.
We use some 15 mil (0.37 mm - thicker wouldn't hurt) high impact polystyrene (HIPS) stock that was lying around to form a specimen support by cutting a piece approximately twice as long as Gary cited, then folding it in half to form an open envelope. The specimen is sandwiched between the folded ends with the fold near the bottom of the chuck and the specimen just protruding from the open end of the sandwich, also just above the microtome jaws. Typically I will trim away the clamped envelope to form a little truncated pyramid at the top and trim the sample to project just above, such that almost all the sample is encased in the HIPS sandwich and the clamp. For specimens that need more support (i.e. very thin films), the disposable nature of this setup comes in handy. I trim away the entire assembly with the specimen inside to form a mesa with HIPS and sample on the face (this is especially helpful when cutting thick sections of multilayer films for light microscopy). The HIPS sides of the pyramid are beveled as well to keep the HIPS sectioning to a minimum. It is generally not a major problem to winnow the sections of interest away from the sectioned support material, but make sure you take the time to get to know the support material microstructure intimately just in case! Good luck!
Yours, Matt
Matthew Stephenson Impact Analytical/MMI 1910 W. Saint Andrews Rd. Midland, MI 48640 (989)832-5555 X506 stephenson-at-impactanalytical.com
-----Original Message----- X-from: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com] Sent: Thursday, January 31, 2008 11:19 AM To: stephenson-at-impactanalytical.com
Sandra,
I agree with Jessica that you should use cryomicrotomy and collect dry sections. This is a challenging technique but with practice you will find it to be valuable. Microtome vendors and other training facilities offer workshops on the cryomicrotomy.
In our lab we use a 'plastic chuck' for sectioning films at ambient and low temperature. The chuck consists of a piece of plastic with approximate dimensions of 4 mm x 7 mm x 3 mm (width x length x thickness). These dimensions should be set to your personal preference and to fit your sample and microtome chuck. Use a stiff plastic such as high density polyethylene or polypropylene that will hold the sample tight but is not rigid or inflexible. Using a sharp razor blade, bisect the 3 mm thickness, cutting through nearly the full length of the plastic piece and leaving a couple of millimeters of at the other end of the piece to hold the arms of the chuck together. The end results is a Y-shaped piece of plastic (see diagram below). To use the chuck, slide the film between the arms of the plastic chuck leaving less than 1 mm exposed; the more film is exposed, the more likely it is to move side-to-side during microtomy. Listers may contact me off-line for a drawing of the chuck.
I recommend that you do not embed films in epoxy if at all possible. The embedding medium, whatever it might be, only contributes to the difficulty of cutting. The problem is that the film has several layers, each with different cutting properties at low temperature; the problem is often even worse at ambient temperature. Adding the embedding medium contributes another medium that most probably has very different cutting properties than any material in the film.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
cervantes-at-bend res.com To gary.m.brown-at-exxonmobil.com 01/30/08 07:40 cc PM Subject [Microscopy] RE: viaWWW: 2 questions Please respond concerning microtomy of thin films to cervantes-at-bend res.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Sandra -
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
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Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yashaa-at-hotmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, February 1, 2008 at 16:42:01 ---------------------------------------------------------------------------
Email: yashaa-at-hotmail.com Name: Rasha ElBashir
Organization: Aachen university of applied science
Education: Graduate College
Location: Aachen, Germany
Question: I need some information for my study about the scanning transmission electron microscope (STEM), how does it work, what is it's principle and other basic information
Please Google "scanning transmission electron microscope" and within the first 10 entries you will be off to a great start.
Roseann
On Feb 1, 2008, at 3:00 PM, yashaa-at-hotmail.com wrote:
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==============================Original Headers============================== 6, 26 -- From RCsencsits-at-lbl.gov Fri Feb 1 17:18:07 2008 6, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m11NI6Wj000454 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 1 Feb 2008 17:18:06 -0600 6, 26 -- X-Ironport-SBRS: None 6, 26 -- X-IronPort-Anti-Spam-Filtered: true 6, 26 -- X-IronPort-Anti-Spam-Result: Ao8CAAo3o0eAAykY/2dsb2JhbACuZQ 6, 26 -- X-IronPort-AV: E=Sophos;i="4.25,292,1199692800"; 6, 26 -- d="scan'208";a="87803098" 6, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 6, 26 -- by ironport1.lbl.gov with ESMTP; 01 Feb 2008 15:18:05 -0800 6, 26 -- Received: from [131.243.35.239] (0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) 6, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id m11NI5QS016516; 6, 26 -- Fri, 1 Feb 2008 15:18:05 -0800 (PST) 6, 26 -- Cc: microscopy-at-microscopy.com 6, 26 -- Message-Id: {5F1C4471-F05A-4969-801F-E3457F60C30D-at-lbl.gov} 6, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 6, 26 -- To: yashaa-at-hotmail.com 6, 26 -- In-Reply-To: {200802012300.m11N0r1G031688-at-ns.microscopy.com} 6, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Mime-Version: 1.0 (Apple Message framework v915) 6, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: info on scanning transmission electron 6, 26 -- Date: Fri, 1 Feb 2008 15:18:04 -0800 6, 26 -- References: {200802012300.m11N0r1G031688-at-ns.microscopy.com} 6, 26 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
There is also a good overview on the FEI site. You can download "All you wanted to know about Electron MIcrosopy.... but didn't dare to ask."
Caveat: MME and The MIP have no commercial interest.
Hope this is helpful. Barbara Foster, President
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through July 2008. Call us today for details.
We are sorry to report that Optimizing Light Microscopy for the Biological and Clinical Laboratory is no longer available.
At 05:29 PM 2/1/2008, RCsencsits-at-lbl.gov wrote:
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Mine is manual insertion and retraction of the solid state STEM detector('s) diodes. The top side of the detector has four diodes and another underneath. Switching amongst the diodes provides BF and DF STEM.
Specimens are held in a standard 3mm diameter TEM grid and are typically imaged at 30KV for highest resolution. Instead of a constant beam of electrons flooding the specimen (like a TEM), the STEM does electron beam scanning of the specimen. Quite a different approach. Sometimes, very good and interesting results.
gary g.
At 02:52 PM 2/1/2008, you wrote:
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==============================Original Headers============================== 9, 21 -- From gary-at-gaugler.com Fri Feb 1 17:52:57 2008 9, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m11NqvpY026274 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 1 Feb 2008 17:52:57 -0600 9, 21 -- Message-Id: {200802012352.m11NqvpY026274-at-ns.microscopy.com} 9, 21 -- Received: (qmail 23015 invoked from network); 1 Feb 2008 15:52:56 -0800 9, 21 -- Received: by simscan 1.1.0 ppid: 23012, pid: 23013, t: 0.0776s 9, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 21 -- by smtp2 with SMTP; 1 Feb 2008 15:52:56 -0800 9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 21 -- Date: Fri, 01 Feb 2008 15:52:54 -0800 9, 21 -- To: yashaa-at-hotmail.com 9, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 21 -- Subject: Re: [Microscopy] AskAMicroscopist: info on scanning 9, 21 -- transmission electron 9, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 21 -- In-Reply-To: {200802012252.m11Mq68E022133-at-ns.microscopy.com} 9, 21 -- References: {200802012252.m11Mq68E022133-at-ns.microscopy.com} 9, 21 -- Mime-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-48833211 ==============================End of - Headers==============================
I learned about the plastic microtomy chuck from Lou Ban, a microscopist who worked at Exxon Chemical some time ago. I've used it for +20 years with success. Thanks, Lou.
You make good points regarding the specifics of use of the chuck during microtomy. Your disposable chuck is ideal for the case in which a cross-section of a thin film is needed for SEM/EDS. After cryo-sectioning or cryo-facing (cryo-planing), the chuck/film assembly can be transferred directly to the SEM for analysis.
We all have insight into what makes a better mousetrap, or in this case, a microtomy chuck. The folks in our lab over the years used the chuck with variations that fit their tastes and needs. Without folk's new ideas and modifications of old ones, man wouldn't be where he is today.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
stephenson-at-imp actanalytical. com To gary.m.brown-at-exxonmobil.com cc 02/01/08 03:51 PM Subject [Microscopy] RE: viaWWW: 2 questions concerning microtomy of Please respond thi to stephenson-at-imp actanalytical. com
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Greetings Sandra,
As usual Gary Brown is dead on with his comments. I agree that embedment is generally best avoided in these multilayer applications. We have seen swelling of the surface layer due to epoxy interaction as well, surprising some real polymer gurus around here. His specimen holder sounds elegant - I think I'll make one. We've taken a more down-and-dirty, and semi-disposable, approach here that may be useful in some of your applications.
We use some 15 mil (0.37 mm - thicker wouldn't hurt) high impact polystyrene (HIPS) stock that was lying around to form a specimen support by cutting a piece approximately twice as long as Gary cited, then folding it in half to form an open envelope. The specimen is sandwiched between the folded ends with the fold near the bottom of the chuck and the specimen just protruding from the open end of the sandwich, also just above the microtome jaws. Typically I will trim away the clamped envelope to form a little truncated pyramid at the top and trim the sample to project just above, such that almost all the sample is encased in the HIPS sandwich and the clamp. For specimens that need more support (i.e. very thin films), the disposable nature of this setup comes in handy. I trim away the entire assembly with the specimen inside to form a mesa with HIPS and sample on the face (this is especially helpful when cutting thick sections of multilayer films for light microscopy). The HIPS sides of the pyramid are beveled as well to keep the HIPS sectioning to a minimum. It is generally not a major problem to winnow the sections of interest away from the sectioned support material, but make sure you take the time to get to know the support material microstructure intimately just in case! Good luck!
Yours, Matt
Matthew Stephenson Impact Analytical/MMI 1910 W. Saint Andrews Rd. Midland, MI 48640 (989)832-5555 X506 stephenson-at-impactanalytical.com
-----Original Message----- X-from: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com] Sent: Thursday, January 31, 2008 11:19 AM To: stephenson-at-impactanalytical.com
Sandra,
I agree with Jessica that you should use cryomicrotomy and collect dry sections. This is a challenging technique but with practice you will find it to be valuable. Microtome vendors and other training facilities offer workshops on the cryomicrotomy.
In our lab we use a 'plastic chuck' for sectioning films at ambient and low temperature. The chuck consists of a piece of plastic with approximate dimensions of 4 mm x 7 mm x 3 mm (width x length x thickness). These dimensions should be set to your personal preference and to fit your sample and microtome chuck. Use a stiff plastic such as high density polyethylene or polypropylene that will hold the sample tight but is not rigid or inflexible. Using a sharp razor blade, bisect the 3 mm thickness, cutting through nearly the full length of the plastic piece and leaving a couple of millimeters of at the other end of the piece to hold the arms of the chuck together. The end results is a Y-shaped piece of plastic (see diagram below). To use the chuck, slide the film between the arms of the plastic chuck leaving less than 1 mm exposed; the more film is exposed, the more likely it is to move side-to-side during microtomy. Listers may contact me off-line for a drawing of the chuck.
I recommend that you do not embed films in epoxy if at all possible. The embedding medium, whatever it might be, only contributes to the difficulty of cutting. The problem is that the film has several layers, each with different cutting properties at low temperature; the problem is often even worse at ambient temperature. Adding the embedding medium contributes another medium that most probably has very different cutting properties than any material in the film.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
cervantes-at-bend res.com To gary.m.brown-at-exxonmobil.com 01/30/08 07:40 cc PM Subject [Microscopy] RE: viaWWW: 2 questions Please respond concerning microtomy of thin films to cervantes-at-bend res.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Sandra -
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both sandra.gardner-at-xerox.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
And if things are so bad then on have to try some of the tricks presented by John, here are two more :
-make a E-4 to E-6 O2 inlet in the pumpe while it's working. It may refresh the Ti surface, by O2 ion reactive etching. Not easy to preform on a microscope. One must have a leakvalve on the scope.
-make a vacuum in the 10E1 - 10 mbar range under air. Same effect that former trick, but with more pressure and not pure oxygen. It's a DC glow discharge. On a microscope it needs to put the vacuum control board in manual mode, and to short some securities... The pump will warm up too. One have together "baking" and ion etching.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
john.mardinly-at-intel.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } There are three little tricks for extending the life of ion pumps that } have not been mentioned yet that I thought I could contribute based on } years of experience with JEOL and Topcon TEM's: } } 1) The hammer. Yes, just smack the bajeezus out of the ion pump body } (not the magnet) with a hammer. The shock knocks most of the whiskers } and flakes loose. Do it with the emitter cold, because it will shock the } whole TEM. Put emphasis on a large number of mild whacks in every } direction of the pump housing. Keep the pump HV on, reading pump current } and you will see lots of little spikes as the whiskers and flakes get } dislodged. Wear hearing protection because you will need to do this for } about 20 minutes. When you stop seeing any spike in ion current with } hammer whacks, you are done. } } 2) Vent the system, pump it down, and restart the ion pump prematurely } with over current protection turned off. The pump should get hot. That's } basically a poor man's bake. It works. } } 3) Hi-pot. This is a technique where the service engineer brings in a } high current ~10KV power supply and connects it to the pump for about an } hour. The high voltage and high current blast all the whiskers and } flakes quite nicely. However, if you have never done this yourself, get } the service engineer to do it and be paranoid because it is VERY } DANGEROUS! If the standard power supply for ion pumps wasn't dangerous } enough, this is one that should treated with the greatest caution. } } John Mardinly, Intel } } This is not necessarily an opinion of Intel Corporation } } -----Original Message----- } X-from: beaurega-at-westol.com [mailto:beaurega-at-westol.com] } Sent: Thursday, January 31, 2008 9:25 AM } To: Mardinly, John } Subject: [Microscopy] Re: ion pump longevity } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi, } } My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., } IMO. } } If you think you have carbon or hydrocarbon issues, have your serviceman } check the long column tubing liner just under the anode cap for a black } coating of residue that builds up on the liner's inner wall. There } should } be a tool in the factory tool kit to remove this tubing liner. Look at } the } amount of the deposit and the color. Ask the serviceman if that amount } of } deposit seems normal or excessive. I would have a supply of 6-8 inch } long } wooden "Q-tip swabs" and Pol polish handy for him. } } HTH, } } Paul } } At 09:01 AM 1/30/08 -0600, you wrote: } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } } ----- } } } Dear listers, } } } } We just changed the IGP pump of the gun chamber only 4 years after the } } } installation of our Tecnai G20. This change was advised by a FEI } engineer. } } } We do not even have 400 working hours on this machine and I wondered if } } } it } was normal that we already have to change the pump. } } } We work mainly with ultrathin sections of biological probes embedded in } } } Epon. They are pretty contaminated with carbon, as evidenced by light } circles remaining on the sections after even a very short illumination } time } (I am usually working at 120 kV, LaB6). I am wondering if this carbon } contamination, evaporated by the electron beam, is not at least partly } responsible for the dirtiness of the pumps (the pump of the column is } bigger than the one for the gun, which would explain why it was not so } dirty). If this is the case, perhaps a plasma cleaner would not only be } a } convenience for me but could bring a financial advantage for my boss (I } know you see what I mean ;-)). } } } What would be the cost of a plasma cleaner? } } What is your opinion on the question? Do you think that a plasma } } } cleaner } would increase the lifetime of the IGPs? } } } Best regards, } } } } Stephane } } } } } } } } } ________________________________________________________________________ } ____ } ________ } } } Looking for last minute shopping deals? } } Find them fast with Yahoo! Search. } } } http://tools.search.yahoo.com/newsearch/category.php?category=shopping } } } ==============================Original } } } Headers============================== } } } 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 } } 6, 18 -- Received: from web37402.mail.mud.yahoo.com } } } (web37402.mail.mud.yahoo.com [209.191.91.134]) } } } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id } m0UF0pGt005637 } } } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 } } } 09:00:53 -0600 } } } 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 } } } 15:00:50 -0000 } } } 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 6, 18 -- s=s1024; d=yahoo.com; } } 6, 18 -- } } } h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:M } essa } ge-ID; } } } 6, 18 -- } } } b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9 } wOTj } IMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXf } nLKD } hyAOWq+S20/9pUAEHFJq8=; } } } 6, 18 -- X-YMail-OSG: } } } OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3 } Ifmh } gAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- } } } 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com } } } via HTTP; Wed, 30 Jan 2008 07:00:50 PST } } } 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 } } 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) } } 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 6, 18 -- To: microscopy-at-microscopy.com } } 6, 18 -- MIME-Version: 1.0 } } 6, 18 -- Content-Type: text/plain; charset=us-ascii } } 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} } } ==============================End of - } } } Headers============================== } } } } } } } ==============================Original } Headers============================== } 6, 16 -- From beaurega-at-westol.com Thu Jan 31 11:24:28 2008 } 6, 16 -- Received: from mail.winbeam.com (mail.winbeam.com } [64.84.96.10]) } 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m0VHOSca002606 } 6, 16 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 10, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Feb 4 07:01:57 2008 10, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.151]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m14D1uSU031600 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 07:01:56 -0600 10, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m14D1qDv064694 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 14:01:52 +0100 (CET) 10, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 49FFD107CE5F 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 Feb 2008 14:01:12 +0100 (CET) 10, 29 -- Message-ID: {47A70C99.7080603-at-ipcms.u-strasbg.fr} 10, 29 -- Date: Mon, 04 Feb 2008 14:01:13 +0100 10, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 10, 29 -- MIME-Version: 1.0 10, 29 -- To: Microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] ion pump longevity more 10, 29 -- References: {200802012057.m11Kv7On031382-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200802012057.m11Kv7On031382-at-ns.microscopy.com} 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-IPCMS-MailScanner: Found to be clean 10, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.151]); Mon, 04 Feb 2008 14:01:52 +0100 (CET) 10, 29 -- X-Virus-Scanned: ClamAV 0.88.7/5678/Mon Feb 4 02:15:53 2008 on mr1.u-strasbg.fr 10, 29 -- X-Virus-Status: Clean 10, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 10, 29 -- version=3.1.8 10, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr1.u-strasbg.fr ==============================End of - Headers==============================
Sorry for the oups! I've forgotten the unit for the first trick, one has to read "E-4 to E-6 mbar O2 (oxygene)". And a little "-" is missing in the second one " 10E-1 to 10 mbar".
It's not a good thing to answer to quickly !
And if things are so bad then on have to try some of the tricks presented by John, here are two more :
-make a E-4 to E-6 mabr O2 inlet in the pump while it's working. It may refresh the Ti surface, by O2 ion reactive etching. Not easy to perform on a microscope. One must have a leakvalve on the scope.
-make a vacuum in the 10E-1 - 10 mbar range under air. Same effect that former trick, but with more pressure and not pure oxygen. It's a DC glow discharge. On a microscope it needs to put the vacuum control board in manual mode, and to short some securities... The pump will warm up too. One have together "baking" and ion etching.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
john.mardinly-at-intel.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } There are three little tricks for extending the life of ion pumps that } have not been mentioned yet that I thought I could contribute based on } years of experience with JEOL and Topcon TEM's: } } 1) The hammer. Yes, just smack the bajeezus out of the ion pump body } (not the magnet) with a hammer. The shock knocks most of the whiskers } and flakes loose. Do it with the emitter cold, because it will shock the } whole TEM. Put emphasis on a large number of mild whacks in every } direction of the pump housing. Keep the pump HV on, reading pump current } and you will see lots of little spikes as the whiskers and flakes get } dislodged. Wear hearing protection because you will need to do this for } about 20 minutes. When you stop seeing any spike in ion current with } hammer whacks, you are done. } } 2) Vent the system, pump it down, and restart the ion pump prematurely } with over current protection turned off. The pump should get hot. That's } basically a poor man's bake. It works. } } 3) Hi-pot. This is a technique where the service engineer brings in a } high current ~10KV power supply and connects it to the pump for about an } hour. The high voltage and high current blast all the whiskers and } flakes quite nicely. However, if you have never done this yourself, get } the service engineer to do it and be paranoid because it is VERY } DANGEROUS! If the standard power supply for ion pumps wasn't dangerous } enough, this is one that should treated with the greatest caution. } } John Mardinly, Intel } } This is not necessarily an opinion of Intel Corporation } } -----Original Message----- } X-from: beaurega-at-westol.com [mailto:beaurega-at-westol.com] } Sent: Thursday, January 31, 2008 9:25 AM } To: Mardinly, John } Subject: [Microscopy] Re: ion pump longevity } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi, } } My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., } IMO. } } If you think you have carbon or hydrocarbon issues, have your serviceman } check the long column tubing liner just under the anode cap for a black } coating of residue that builds up on the liner's inner wall. There } should } be a tool in the factory tool kit to remove this tubing liner. Look at } the } amount of the deposit and the color. Ask the serviceman if that amount } of } deposit seems normal or excessive. I would have a supply of 6-8 inch } long } wooden "Q-tip swabs" and Pol polish handy for him. } } HTH, } } Paul } } At 09:01 AM 1/30/08 -0600, you wrote: } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } } ----- } } } Dear listers, } } } } We just changed the IGP pump of the gun chamber only 4 years after the } } } installation of our Tecnai G20. This change was advised by a FEI } engineer. } } } We do not even have 400 working hours on this machine and I wondered if } } } it } was normal that we already have to change the pump. } } } We work mainly with ultrathin sections of biological probes embedded in } } } Epon. They are pretty contaminated with carbon, as evidenced by light } circles remaining on the sections after even a very short illumination } time } (I am usually working at 120 kV, LaB6). I am wondering if this carbon } contamination, evaporated by the electron beam, is not at least partly } responsible for the dirtiness of the pumps (the pump of the column is } bigger than the one for the gun, which would explain why it was not so } dirty). If this is the case, perhaps a plasma cleaner would not only be } a } convenience for me but could bring a financial advantage for my boss (I } know you see what I mean ;-)). } } } What would be the cost of a plasma cleaner? } } What is your opinion on the question? Do you think that a plasma } } } cleaner } would increase the lifetime of the IGPs? } } } Best regards, } } } } Stephane } } } } } } } } } ________________________________________________________________________ } ____ } ________ } } } Looking for last minute shopping deals? } } Find them fast with Yahoo! Search. } } } http://tools.search.yahoo.com/newsearch/category.php?category=shopping } } } ==============================Original } } } Headers============================== } } } 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 } } 6, 18 -- Received: from web37402.mail.mud.yahoo.com } } } (web37402.mail.mud.yahoo.com [209.191.91.134]) } } } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id } m0UF0pGt005637 } } } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 } } } 09:00:53 -0600 } } } 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 } } } 15:00:50 -0000 } } } 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 6, 18 -- s=s1024; d=yahoo.com; } } 6, 18 -- } } } h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:M } essa } ge-ID; } } } 6, 18 -- } } } b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9 } wOTj } IMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXf } nLKD } hyAOWq+S20/9pUAEHFJq8=; } } } 6, 18 -- X-YMail-OSG: } } } OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3 } Ifmh } gAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- } } } 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com } } } via HTTP; Wed, 30 Jan 2008 07:00:50 PST } } } 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 } } 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) } } 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 6, 18 -- To: microscopy-at-microscopy.com } } 6, 18 -- MIME-Version: 1.0 } } 6, 18 -- Content-Type: text/plain; charset=us-ascii } } 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} } } ==============================End of - } } } Headers============================== } } } } } } } ==============================Original } Headers============================== } 6, 16 -- From beaurega-at-westol.com Thu Jan 31 11:24:28 2008 } 6, 16 -- Received: from mail.winbeam.com (mail.winbeam.com } [64.84.96.10]) } 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m0VHOSca002606 } 6, 16 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 } 11:24:28 -0600 } 6, 16 -- Received: (qmail 16520 invoked by uid 89); 31 Jan 2008 17:24:26 } -0000 } 6, 16 -- Received: from pitts-69-72-117-2.dynamic-dialup.coretel.net } (HELO millenium) (69.72.117.2) } 6, 16 -- by mail.winbeam.com with SMTP; 31 Jan 2008 17:24:26 -0000 } 6, 16 -- Message-Id: } {3.0.6.32.20080131122424.007b6420-at-pop3.norton.antivirus} } 6, 16 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus } (Unverified) } 6, 16 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) } 6, 16 -- Date: Thu, 31 Jan 2008 12:24:24 -0500 } 6, 16 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com } 6, 16 -- From: Beaurega {beaurega-at-westol.com} } 6, 16 -- Subject: Re: [Microscopy] ion pump longevity } 6, 16 -- Mime-Version: 1.0 } 6, 16 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 19, 32 -- From john.mardinly-at-intel.com Fri Feb 1 14:48:11 2008 } 19, 32 -- Received: from mga11.intel.com (mga11.intel.com [192.55.52.93]) } 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m11KmAUv022712 } 19, 32 -- for {Microscopy-at-msa.microscopy.com} ; 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-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
I was asked by a biological microscopist for information pertaining to preparation and microtomy of polymer films and materials. Like many of us, we are being asked to broaden our area of expertise into unfamiliar territory. The following suggestions are based on guidance by several other microscopists and much experience over the years. I don't claim to be an expert, only a microscopist with a few proven tricks up my sleeve. This is not a comprehensive or authoritative monograph, just friendly advice. My hope is that this will cause a flurry of responses with additional suggestions and ideas, questions (and answers) on preparation of other sample types, and correction of any wrong statements by me.
Like biological microscopy, material science offers many challenges. The broad spectrum of materials often requires only several key preparation and microscopy tools and techniques. I would start with the materials that you are presented with and worry about other potential challenges when they arise. That said, there are several approaches to microtomy of materials that are worth mentioning. As in every other aspect of microscopy, sample preparation is absolutely essential.
Frankly, I learned most of the methods that I used by trial and error with help from a few colleagues. I'm sure that a several if not many good review papers and books deal with preparation techniques for catalyst-related materials such as zeolites, aluminas, silicas, etc. These are exceptionally important materials used to support catalyst species. The microtome vendors can provide much insight into preparation of materials for microscopy. Alternatively, other organizations such as McCrone Associates may offer workshop.
Consider polishing if larger flat surfaces of hard materials are needed for SEM analysis. Look for contract labs that will grind and polish your samples until you have a sufficiently high sample volume that you need to invest in grinding and polishing equipment.
Microtomy is the key to successful analysis of polymers. Several points follow: I generally try to avoid embedment. Exceptions to this are fibers, fabrics and tiny particles that require rigid supports during microtomy. When embedment is unavoidable, try to match the hardness of the medium to the sample as best possible. IMPORTANT: Do not cure any embedding medium at a temperature approaching the polymer melting point. If you don't know the melting temperature, use an ambient curing epoxy such as EpoFix. Polyolefins, a major class of polymers including polyethylene, polypropylene, etc., require special care since their melting temperatures can be as low as 40-50C or as high as 130-170C. Embedded or not, section the polymer at a temperature below it's glass transition temperature (Tg). When uncertain, lower the cutting temperature to { -130C. Cryo-section slowly since friction induced at higher rates can cause heating of the polymer above it's Tg where it will soften and plastically deform.
I use a standard approach to microtomy of hard materials like minerals and catalyst supports. Low viscosity is an important requirement for any embedding medium for small particles. I generally use LR White - hard grade - without the accelerator because it is has very low viscosity, is devoid of accelerator, is easy to use. The low viscosity allows it to readily infiltrate most catalyst support particles giving good embedment. Oven cure LR White at 80-90C for several hours in a nitrogen-purged oven. On occasions, I will use EpoFix epoxy. Call your supplier of embedding resins to find an epoxy:accelerator mixture that will give a hard block. Cure at as high a temperature as is recommended by the manufacturer or your supplier. If the material is not in a finely powered form and grinding permits, use a garnet mortar and pestle to finely divide the material. Sprinkle the powder toward one end of a flat embedding mold. Take care to have good density of particles in the mold but avoid piles or clumps of catalyst since they may not be well infiltrated with the embedding medium. Degas the embedding medium in a vacuum to remove bubbles and absorbed air. Epoxies and other higher viscosity embedding resins will take longer to degas than lower viscosity resins like LR White. Slowly layer the degassed embedding medium into the mold. Fully cover the sample and fill the mold. Identify the sample with a small paper label printed in pencil (ink runs when exposed to epoxy or LR White) in the opposite end of the mold from the sample. Place the mold plus catalyst into a vacuum and infiltrate for half an hour or so. If the embedding resin begins to foam due to release of air from within the powder, gently break vacuum several times until the material is stable in the vacuum. Heat cure in a nitrogen-purged oven for the required time.
Best regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
==============================Original Headers============================== 12, 18 -- From gary.m.brown-at-exxonmobil.com Mon Feb 4 10:24:49 2008 12, 18 -- Received: from hoespc02.exxonmobil.com (hoespc02.exxonmobil.com [158.35.223.2]) 12, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m14GOm4m030674 12, 18 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 10:24:49 -0600 12, 18 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 12, 18 -- by hoespc02.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m14GOk97021184 12, 18 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 10:24:47 -0600 (CST) 12, 18 -- Subject: Microtomy of polymer films and hard particles 12, 18 -- Importance: 12, 18 -- To: microscopy-at-microscopy.com 12, 18 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 12, 18 -- Message-ID: {OFB8D03867.228FDDFC-ON862573E5.0058B16B-862573E5.005A27DB-at-exxonmobil.com} 12, 18 -- From: gary.m.brown-at-exxonmobil.com 12, 18 -- Date: Mon, 4 Feb 2008 10:24:44 -0600 12, 18 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 12, 18 -- 02, 2006) at 02/04/2008 10:24:47 AM 12, 18 -- MIME-Version: 1.0 12, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
We have a job opening for Director of the Imaging Technology Group ITG has a job opening for a new director.
The Beckman Institute at the University of Illinois, Urbana Champaign has a position opening for one (1) Director, Imaging Technology Group (ITG). The ITG's primary mission is to provide state-of-the-art imaging and visualization resources for researchers at the Institute and the University of Illinois. This mission is accomplished through two facilities: a Microscopy Suite, and a Visualization, Media, and Imaging Laboratory. A secondary mission of the ITG is to develop advanced technologies with an emphasis on projects in remote and virtual instrumentation. The ITG Director sets the vision for this group of approximately 25 staff members, writes grants and directs special projects to support that vision, and conducts daily management of the staff.
Read the announcement: http://www.itg.uiuc.edu/announcements/director.htm and if you have questions, please contact jobs-at-beckman.uiuc.edu.
Cheers,
Jonathan M. Ekman Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 N. Mathews Avenue Urbana, IL 61801 USA Tel: 217-244-6292 Fax: 217-244-6219
==============================Original Headers============================== 7, 19 -- From jekman-at-uiuc.edu Mon Feb 4 14:11:24 2008 7, 19 -- Received: from expredir3.cites.uiuc.edu (expredir3.cites.uiuc.edu [128.174.5.186]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m14KBNju018540 7, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 14:11:23 -0600 7, 19 -- Received: from rollinsitg (rollins.itg.uiuc.edu [130.126.126.232]) 7, 19 -- by expredir3.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id m14KBMnq017277 7, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 14:11:22 -0600 (CST) 7, 19 -- From: "Jon Ekman" {jekman-at-uiuc.edu} 7, 19 -- To: {Microscopy-at-microscopy.com} 7, 19 -- Subject: Job opening for Director of the Imaging Technology Group 7, 19 -- Date: Mon, 4 Feb 2008 14:11:22 -0600 7, 19 -- Message-ID: {000d01c8676a$1cd6c290$e87e7e82-at-rollinsitg} 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; 7, 19 -- charset="us-ascii" 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- X-Mailer: Microsoft Office Outlook 11 7, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 7, 19 -- Thread-Index: AchnahzBmSMGV2XiT/2U23oeuKbtzg== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT
Title-Subject: [Filtered] grease for Sample exchange holder JEOL 8600 Superprobe.
Question: Hello all, I am using a JEOL JXA 8600 superprobe at the moment. Unfortunately, the main microscopist is not available to help me with my query. I am having a problem with sample exchange, as the rod doesn't slide freely in and out of the exchange chamber. I have cleaned/degreased the rod and re-greased again with the lubricant that is there (no brand name available!) but after putting the sample in place, the rod becomes black and sticks on removal. As I am relatively new to microscopy - I would like some advice from someone with a similar instrument regarding the correct grease to use for this situation.
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Email: yongli-at-mcb.harvard.edu Name: Yong Li
Organization: Harvard University
Title-Subject: [Filtered] service for LKB 8800 Ultrotome III
Question: Hi,
There are a LKB 8800 Ultrotome III and its controller LKB 8802A in the lab and they need to be serviced. But as LKB has disappeared, I can't find any company to service on these two machines. Does anyone know a company that does services on the LKB machines?
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Email: jhuisken-at-gmail.com Name: Jan Huisken
Organization: UCSF
Title-Subject: [Filtered] Filter sets for Leica MZ16 F
Question: Hi there,
We are planning on upgrading our Leica Stereoscopes (MZ16F) with new filter sets. It turned out that Leica charges almost twice as much as we would pay at Chroma for just the glass (ca. $1400 vs. $750). Is anybody aware of a company that sells the empty filter holder so that we can mount the filters ourselves?
For a use such as the Jeol SEM's rod, I use Dow Corning High Vacuum Grease. It's a silicon type grease. I do not use Apezon (L or M) vacuum greases for dynamic use, only for static seals. Apiezon greases become sticky, and hard. Silicon grease outgases a bit when fresh, but slides very well. I suppose you may find other brands too (Nye, Monsanto or others). Some people use a dropplet of dif pump oil (DC704/705), but it needs to be cleaned and re-oiled more frequently.
The black traces may be dirt loosen by the freshly greased rod. In case, clean it away with ethanol or aceton, and but a new set of (new) grease. Wip away all grease which could accumulate at the end of the rod. It will be only a dust trap !
I would not use an "unkown" brand of grease, unless you know it has be given by the manufacturer of the intrument for that use.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
emer.ryan-at-dit.ie a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: emer.ryan-at-dit.ie } Name: Emer Ryan } } Organization: CREST DIT } } Title-Subject: [Filtered] grease for Sample exchange holder JEOL 8600 } Superprobe. } } Question: Hello all, } I am using a JEOL JXA 8600 superprobe at the moment. Unfortunately, } the main microscopist is not available to help me with my query. } I am having a problem with sample exchange, as the rod doesn't slide } freely in and out of the exchange chamber. } I have cleaned/degreased the rod and re-greased again with the } lubricant that is there (no brand name available!) but after putting } the sample in place, the rod becomes black and sticks on removal. } As I am relatively new to microscopy - I would like some advice from } someone with a similar instrument regarding the correct grease to use } for this situation. } } Thanks in advance. } } } Login Host: 147.252.65.57 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Feb 5 01:24:51 2008 } 8, 11 -- Received: from [10.1.1.21] (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m157OfHB002999 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 01:24:44 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c3cdbfa665aa-at-[10.1.1.21]} } 8, 11 -- Date: Tue, 5 Feb 2008 01:24:40 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: grease for Sample exchange holder JEOL 8600 Superprobe } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Feb 5 05:05:59 2008 10, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m15B5w9j015729 10, 30 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 05:05:59 -0600 10, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 30 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m15B5tSO063782 10, 30 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 12:05:55 +0100 (CET) 10, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 405767E4038 10, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 12:05:06 +0100 (CET) 10, 30 -- Message-ID: {47A842E9.2060007-at-ipcms.u-strasbg.fr} 10, 30 -- Date: Tue, 05 Feb 2008 12:05:13 +0100 10, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 30 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 10, 30 -- MIME-Version: 1.0 10, 30 -- To: Microscopy-at-microscopy.com 10, 30 -- Subject: Re: [Microscopy] viaWWW: grease for Sample exchange holder JEOL 8600 10, 30 -- Superprobe 10, 30 -- References: {200802050735.m157Zpfi028274-at-ns.microscopy.com} 10, 30 -- In-Reply-To: {200802050735.m157Zpfi028274-at-ns.microscopy.com} 10, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 30 -- Content-Transfer-Encoding: 8bit 10, 30 -- X-IPCMS-MailScanner: Found to be clean 10, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.152]); Tue, 05 Feb 2008 12:05:55 +0100 (CET) 10, 30 -- X-Virus-Scanned: ClamAV 0.88.7/5692/Tue Feb 5 08:21:55 2008 on mr2.u-strasbg.fr 10, 30 -- X-Virus-Status: Clean 10, 30 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 10, 30 -- version=3.1.8 10, 30 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr2.u-strasbg.fr ==============================End of - Headers==============================
The College of Microscopy, located in Westmont, IL, is offering the following electron microscopy short courses:
March 25 to 27 - Transmission Electron Microscopy
March 31 to April 4 - Scanning Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Tue Feb 5 09:15:13 2008 11, 27 -- Received: from pgp.mccrone.com (65-43-97-126.ded.ameritech.net [65.43.97.126]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m15FFD3G007402 11, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 09:15:13 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 4DF3E1A801A 11, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 09:15:14 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Tue, 05 Feb 2008 09:15:14 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Subject: Short Course Announcement: TEM and SEM 11, 27 -- Date: Tue, 5 Feb 2008 09:15:07 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC3D-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Short Course Announcement: TEM and SEM 11, 27 -- Thread-Index: AchoCePUhJHnuV+dQFaDzT0uNKb/Tg== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {microscopy-at-microscopy.com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m15FFD3G007402 ==============================End of - Headers==============================
As the topic is still discussed, here are a few sentences on our experience:
For an IGP pump, four years is short. 400 working hours far too short. On our FEI CM12, after 17 years of use, always at 120 keV, about 48 weeks each year and } 40 hours each week, at least 10 or sometimes 15 different users per year, we only have the second IGP now in place, since about 3 or 4 years. We have a TEM with a good vacuum, indeed. Since end 1999, we have a slow-scan CCD - so we do not use film any more. So the TEM is always on and under vacuum, at least 360 days per year. LaB6 filament: changed once in 3 years, only, although frequently used. What we do: we always use LN2, every day. Every evening, we shut down the IGP, for four hours, during the time when the anti-contaminator is warming up. This is part of the software package we have (v.12.5).
This extends the lifetime of the IGP, we were told, by preventing the water, which was trapped at the anti-contaminator, from reaching the IGP. - This apparently helps to keep a good vacuum status, in general.
I have no financial interest in FEI - just a satisfied customer. best regards - and hopefully always a good vacuum, Reinhard
--
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 8, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Tue Feb 5 14:47:56 2008 8, 23 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m15KltU0003015 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 14:47:56 -0600 8, 23 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 8, 23 -- by localhost (Postfix) with SMTP id 818BD55FA3 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 21:48:03 +0100 (CET) 8, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 8, 23 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id 7433D5607B 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 21:48:03 +0100 (CET) 8, 23 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 8, 23 -- with Novell_GroupWise; Tue, 05 Feb 2008 21:47:54 +0100 8, 23 -- Message-Id: {47A8D8F3.044C.0054.0-at-biologie.uni-regensburg.de} 8, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 8, 23 -- Date: Tue, 05 Feb 2008 21:45:23 +0100 8, 23 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 8, 23 -- To: {Microscopy-at-Microscopy.Com} 8, 23 -- Subject: ion pump longevity 8, 23 -- Mime-Version: 1.0 8, 23 -- Content-Type: text/plain; charset=US-ASCII 8, 23 -- Content-Disposition: inline 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m15KltU0003015 ==============================End of - Headers==============================
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
I have a book that covers all kinds of fiber fracture and damage, polymeric and natural ones. It's an atlas with lots of images and descriptions of the failure mode:
Atlas of fibre fracture and damage to textiles. Second Edition. Hearle J W S, Lomas B & Cooke W D. Woodhead Publishing. ISBN 1 85573 319 6
Best regards,
Petra __________________________________ Dr. Petra Wahlbring Lead Engineer Analytical Test Lab GTC*L Colmar-Berg, Luxembourg e-mail: petra.wahlbring-at-goodyear.com Tel. +352 8199 3725 Fax. +352 8199 5643
- May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the express written consent of The Goodyear Tire & Rubber Company. -
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
I am trying to prepare stripped and spread red blood cell skeletons for negative stain EM. Briefly it involves isolating red blood cells through centrifugation, hypotonic lysis to remove the hemoglobin, Triton X-100 extraction, and sucrose density gradient centrifugation under high salt conditions. This should yield the basic skeleton of the red blood cell which contains actin, spectrin, and 4.1. I am following the protocol by Shen et al (Ultrastructure of unit fragments of the skeleton of the human erythrocyte membrane. J. Cell. Biol. 99:810-821).
The problem I am having is that the skeletons I am isolating are aggregating on the grids, and I am not sure if it is a problem with my sample preparation protocol, my stain, or the grids. I've tried different support films (holey and continuous, plastic and carbon) and different negative stains (UA and Ammonium molybdate of different concentrations). The grids have been both glow-discharged and non-glow-discharged. Diluting the sample with buffer does not remedy the problem. Is there a chance my prep is aggregating in solution? Some protocols add a little DTT to their suspension so I will next experiment with that. If there is anyone who has experience preparing these skeletons, any pointers would be greatly appreciated. Thank you.
Andrea Nans Graduate Student NYU Med Center
==============================Original Headers============================== 4, 27 -- From andrea.nans-at-gmail.com Wed Feb 6 10:18:43 2008 4, 27 -- Received: from mu-out-0910.google.com (mu-out-0910.google.com [209.85.134.187]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m16GIgwo001754 4, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Feb 2008 10:18:42 -0600 4, 27 -- Received: by mu-out-0910.google.com with SMTP id g7so2529651muf.0 4, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 06 Feb 2008 08:18:41 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=MwZSAoi9R9uiFpjlI+xlIDpFDz+x4bkjcbdubnCYNds=; 4, 27 -- b=Z6ctCGjMqGcwrjqmFShqSjAMcQJhZWlOcdyNtIT5zQGQq42jWi83vLPGhw8iYlmDy4fZ9nROmmufvH0WMffTSzc7T3vh7u9iQvVoMx4uoS9kxieLyP4G6OfrZBaJ/YYjpd92/Zrj8JIeywtSOuZM0TUCW5Db2n4t5eJS0TcU9Kc= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=Iom8NtV0LwEIqbCtySHnT6WZOEHEBOpAUxmOmGe/fUaI8Elrpel4An3ZoIu6yMRenFKnJTXsSkDtNKNlLHnz8/TC/o3paD/hgBqaxxLIk5xTSYq2oZ6vVfvdvdhfTJmjzyKvqMtgyop61FINmohLaejdm1uVX6L57J+m1nthCT0= 4, 27 -- Received: by 10.82.159.15 with SMTP id h15mr18250597bue.36.1202314721523; 4, 27 -- Wed, 06 Feb 2008 08:18:41 -0800 (PST) 4, 27 -- Received: by 10.82.179.4 with HTTP; Wed, 6 Feb 2008 08:18:41 -0800 (PST) 4, 27 -- Message-ID: {f4f4e6200802060818o54a61c4w6b122e870c042861-at-mail.gmail.com} 4, 27 -- Date: Wed, 6 Feb 2008 11:18:41 -0500 4, 27 -- From: "Andrea Nans" {andrea.nans-at-gmail.com} 4, 27 -- To: Microscopy-at-Microscopy.Com 4, 27 -- Subject: TEM Sample Prep- Red Cell Skeleton Spread 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I am thinking about buying a Wacom Cintiq monitor/tablet so that I can trace objects during on the screen for Photoshop operations (e.g., area measurements using the Fovea Pro toolkit). I would welcome comments from those using this approach about its ease and success rate. The 6 x 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any thoughts on the size of the tablet or the DTF vs. Cintiq models would also be useful. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Lothar Engel et al., An Atlas of Polymer Damage - Surface Examination by Scanning Electron Microscope, Prentice-Hall, New Jersey (1981) has been useful to me. It is not comprehensive but helped in understanding the morphology of polyolefin failure as seen using optical, SEM and TEM.
Regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
yuhong.wu-at-solv ay.com To gary.m.brown-at-exxonmobil.com 02/05/08 11:25 cc PM Subject [Microscopy] viaWWW: Polymer Failure Please respond Analysis book to yuhong.wu-at-solv ay.com
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
This Question was submitted to Ask-A-Microscopist by (mcaruso2-at-uiuc.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, February 6, 2008 at 17:31:31 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both mcaruso2-at-uiuc.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: mcaruso2-at-uiuc.edu Name: Mary M Caruso
Organization: University of Illinois at Urbana-Champaign
Education: Graduate College
Location: Urbana, IL, USA
Title: Stain for imaging amines in epoxy resin
Question: Do you know of a stain for electron microscopy that is functional group specific? We would like to add a stain or dye that would tag amines in an epoxy matrix to show that some of them have not completely reacted in the 3D polymer network. I tried ninhydrin as a stain and can see the purple color appear on the surface of the resin but imaging these were more difficult. Thank you for your help. ~Mary
We used MetaMorph back at UCL and I bought a small $100 Wacom Graphire version 2 A5 Tablet for image editing and tracing regions. The pen with this set was superior to the latest (cheaper) Graphire v4 as the buttons were located further down the pen as a single rocker and using the buttons was far easier - which really affects how nice it is to use the tablet. The old style pen looked more like the far more expensive Intuos 3 style. We didn't need any larger than the little A5 pad for drawing around images of cells/tissue on 22 inch CRT screen (any photos/negatives were scanned into the PC rather than placed under the tablet to trace over). I would be interested to hear from any who found the larger A4/A3 Wacon Intuos useful for tracing (we don't do 'graphics' with it at work, it's always just tracing, although my kids use a Graphire 2 with Corel Painter X at home).
Initially the works Wacom Graphire 2 tablet was hardly used and I disliked it as the pen (and Wacom mouse) was so poor for basic Windows chores. However when I had hundreds of cells to trace the Wacom Graphire 2 pad came into it's own and it really helped tracing speed and accuracy (I just wouldn't use a mouse to trace now if the pad was available). That said you need the PC optical mouse as well to use menus etc.., they work happily with the pen, you just drop the pen into it's holder and take over the curser with the optical mouse when you have finished tracing (the pen providing the double click to complete the trace).
When an unknown user lost the Graphire Pen (it looks like a normal pen and probably got thrown away) we bought a cheap Graphire 4 pad - but I was really unhappy with the pen button locations and ended up paying £30 for a replacement Graphire 2 pen and used that pad instead (may just be habit). The graphire 4 was half the price of the Graphire 2 (which did include a rather naf mouse). So I would be tempted to go upmarket beyond the $80 Graphire 4, and go for something like an Intuos - and we found the smaller A5 size perfect to fit on the desktop with a MS Intellimouse mouse.
I think an A4 tablet might have taken up too much room, but we bought the little A5 Graphire 2 on a tight budget to try it out and never felt the need to upgrade. I think the Cintiq may be going over the top just to trace objects (and you can use the Intuos pen with the Cintiq if you upgrade later) - but if that's all someone ever does in the lab, why not. Personally I like using my PC monitor with a space saving A5 Wacom pad (that’s the same size as the mouse pad). Our old Magiscan image analyzer back in the late 1980s had a light pen for drawing on the CRT screen (push on the glass screen to 'mouse click') for image editing and that worked really well [largely as the Magiscan image editing software was so good], so an expensive Cintiq should be very successful as well. I did look into light pens but they are expensive and aren't so software/LCD screen friendly as tablets.
Enlarging the object [Zoom/magnify] onscreen really helped with accurate tracing, and metaMorph has a useful back untrace function if you slip too far off target. Some software just zooms in the area where you tracing, which can be useful (I think ImagePro Plus v4/5 [Bio-rad LaserPix] did that, but I can't remember exactly).
So the very cheap A5 wacom tablet was a success for us for occasional tracing of microscope images - nearly always for tracing around cells from wide field phase contrast [transmission] optical microscope images, eg. For cell extention/retraction studies during wound healing. Have you tried a cheaper Wacom Intuos 3 tablet and are wanting to upgrade for some reason ?
Regards
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: 06 February 2008 16:36 To: kjmorris-at-well.ox.ac.uk
I am thinking about buying a Wacom Cintiq monitor/tablet so that I can trace objects during on the screen for Photoshop operations (e.g., area measurements using the Fovea Pro toolkit). I would welcome comments from those using this approach about its ease and success rate. The 6 x 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any thoughts on the size of the tablet or the DTF vs. Cintiq models would also be useful. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
I'm looking for a control box for my Ultracut E ultramicrotome. If anyone has one they would like to sell please contact me. Thanks.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 4, 20 -- From tbargar-at-unmc.edu Thu Feb 7 09:35:00 2008 4, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17FZ0fH024002 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 7 Feb 2008 09:35:00 -0600 4, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 4, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id DFB89A0062 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 7 Feb 2008 09:34:57 -0600 (CST) 4, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 4, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id 6B64739804A 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 7 Feb 2008 09:34:56 -0600 (CST) 4, 20 -- Subject: looking for an Ultracut E control box 4, 20 -- To: Microscopy-at-MSA.Microscopy.com 4, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 4, 20 -- Message-ID: {OF1DB1D819.63B728E6-ON862573E8.00555EA9-862573E8.0055982B-at-unmc.edu} 4, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 4, 20 -- Date: Thu, 7 Feb 2008 09:34:55 -0600 4, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 02/07/2008 09:34:56 4, 20 -- AM 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
On Feb 7, 2008, at 3:40 AM, kjmorris-at-well.ox.ac.uk wrote:
} I have not used the Cintiq (I don't have that kind of money), but I have been using the Wacom Intuos for years now. For tracing objects it is fantastic. For use with photoshop it is great. I have found an added benefit. Switching between the 'pen' and the mouse has alleviated some of my carpal tunnel problems. The improvement has been great enough I got one for home also. Another advantage for me is that I now have a mouse with 5 buttons that can be individually programed for different applications. I have gotten very used to this convenience. It is hard to go back to the one button Mac mouse :-) I find the Wacom mouse (I have only used it on the larger tablets) to be very usable.
David } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am thinking about buying a Wacom Cintiq monitor/tablet so that I can } trace objects during on the screen for Photoshop operations (e.g., } area } measurements using the Fovea Pro toolkit). I would welcome comments } from those using this approach about its ease and success rate. The } 6 x } 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any } thoughts on the size of the tablet or the DTF vs. Cintiq models would } also be useful. Thanks, Tom } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } } } ==============================Original } Headers============================== } 6, 23 -- From PhillipsT-at-missouri.edu Wed Feb 6 10:23:14 2008 } 6, 23 -- Received: from um-nsmtpout1.um.umsystem.edu } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m16GNEgD004656 } 6, 23 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 6 Feb 2008 } 10:23:14 -0600 } 6, 23 -- Received: from UM-XMAIL06.um.umsystem.edu } ([209.106.228.32]) by } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 6, 23 -- Wed, 6 Feb 2008 10:23:13 -0600 } 6, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 6, 23 -- Content-class: urn:content-classes:message } 6, 23 -- MIME-Version: 1.0 } 6, 23 -- Content-Type: text/plain; } 6, 23 -- charset="us-ascii" } 6, 23 -- Subject: Wacom tablets/touch-screen monitors } 6, 23 -- Date: Wed, 6 Feb 2008 10:23:11 -0600 } 6, 23 -- Message-ID: } {0510DC719E56F64BB2AD84EE64CE6BAD03726878-at-UM-XMAIL06.um.umsystem.edu} } 6, 23 -- X-MS-Has-Attach: } 6, 23 -- X-MS-TNEF-Correlator: } 6, 23 -- Thread-Topic: Wacom tablets/touch-screen monitors } 6, 23 -- Thread-Index: Acho3JDHWj+Txz4CR9u0MVm9e7Fvlw== } 6, 23 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 6, 23 -- To: {Microscopy-at-msa.microscopy.com} } 6, 23 -- X-OriginalArrivalTime: 06 Feb 2008 16:23:13.0653 (UTC) } FILETIME=[924AD250:01C868DC] } 6, 23 -- Content-Transfer-Encoding: 8bit } 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m16GNEgD004656 } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 25, 23 -- From kjmorris-at-well.ox.ac.uk Thu Feb 7 04:36:05 2008 } 25, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 25, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m17Aa4Xs031815 } 25, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Feb 2008 04:36:05 } -0600 } 25, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 25, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 25, 23 -- id 1JN46u-0000lC-E5 } 25, 23 -- for Microscopy-at-Microscopy.Com; Thu, 07 Feb 2008 10:36:04 } +0000 } 25, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 25, 23 -- To: {Microscopy-at-Microscopy.Com} } 25, 23 -- References: {200802061635.m16GZtDL025918-at-ns.microscopy.com} } 25, 23 -- Subject: RE: [Microscopy] Wacom tablets/touch-screen } monitors } 25, 23 -- Date: Thu, 7 Feb 2008 10:36:24 -0000 } 25, 23 -- Message-ID: {000801c86975$49d64b50$b22c4381-at-CytoWhizz} } 25, 23 -- MIME-Version: 1.0 } 25, 23 -- Content-Type: text/plain; } 25, 23 -- charset="iso-8859-1" } 25, 23 -- X-Mailer: Microsoft Office Outlook 11 } 25, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 25, 23 -- Thread-Index: Acho3llI7zRt/XwiRjyXX6VRewlknAAiwREg } 25, 23 -- In-Reply-To: {200802061635.m16GZtDL025918-at-ns.microscopy.com} } 25, 23 -- Content-Transfer-Encoding: 8bit } 25, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m17Aa4Xs031815 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Thu Feb 7 09:54:53 2008 5, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.Arizona.EDU [128.196.133.168]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17FsrZh004883 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 7 Feb 2008 09:54:53 -0600 5, 22 -- Received: from frodos_amavis (amavis7.email.arizona.edu [10.0.0.235]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 97DC3290775 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 7 Feb 2008 08:54:52 -0700 (MST) 5, 22 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id B57F428C5A5 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 7 Feb 2008 08:54:44 -0700 (MST) 5, 22 -- Message-Id: {BCEA8D2D-9479-4951-B862-397D3CC039B0-at-arizona.edu} 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- In-Reply-To: {200802071040.m17Aeku2004223-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Mime-Version: 1.0 (Apple Message framework v915) 5, 22 -- Subject: Re: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 08:54:31 -0700 5, 22 -- References: {200802071040.m17Aeku2004223-at-ns.microscopy.com} 5, 22 -- X-Mailer: Apple Mail (2.915) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Memorial University of Newfoundland, located in St. John's, is pleased to announce a short course on "Mineral Liberation Analysis" this spring, May 12-14, as well as an advanced course May 15-16. The courses are aimed at minerals industry professionals and attendance is limited. More information can be found in the announcement broshure: http://www.mun.ca/creait/maf/MUN_MLA_Short_Course_2008_2.pdf
... and at our Micro-analysis Facility's website: http://www.mun.ca/creait/maf/index.php and http://www.mun.ca/creait/maf/mla.php
Briefly, "Mineral Liberation Analysis" (MLA) refers primarily to economic minerals and quantifying the degree to which they're liberated (or locked) relative to other non-economic minerals. However, these specific mineral relationships are only a subset of mineral associations for which the MLA is also quite capable of quantifying. Other applications include point-counting to rare-phase searches. In the past, MLA instrumentation has been employed primarily at minerals' industry research locations worldwide; however, has recently found its way into university research in the last several years.
"Mineral Liberation Analysis" is a software solution that automates the SEM's BEI and stage, as well as integrates high-speed x-ray spectral acquisition (e.g., dual Bruker SDD x-ray detectors). The software was developed at the University of Queensland's Julius Kruttschnitt Mineral Research Centre (JKMRC), which is now the technology transfer company JKTech Pty Ltd: http://www.jktech.com.au/Products_Services/MLA/index.htm
Genuinely, Michael Shaffer :o) SEM-MLA Research Coordinator INCO Innovation Ctr Memorial University 230 Elizabeth Avenue St. John's Newfoundland A1C5S7 (709) 737-6799 (Ofc & Msg) (709) 737-6790 (SEM Lab) (709) 737-6193 (FAX) http://www.mun.ca/creait/maf/
==============================Original Headers============================== 6, 27 -- From michael-at-shaffer.net Thu Feb 7 10:06:33 2008 6, 27 -- Received: from smtprelay.hostedemail.com (smtprelay0062.hostedemail.com [216.40.44.62]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17G6WQX017640 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 10:06:33 -0600 6, 27 -- Received: from emd2-omf02.hostedemail.com (ff-bigip1 [10.5.19.254]) 6, 27 -- by smtprelay01.hostedemail.com (Postfix) with ESMTP id 907F8FB9EF 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 16:06:31 +0000 (UTC) 6, 27 -- X-SpamScore: 50 6, 27 -- X-Spamcatcher-Summary: 50,0,0,418fa7ea1b279db1,adfbb84ca675e7e0,michael-at-shaffer.net,-,RULES_HIT:10:355:379:539:541:542:967:973:988:989:1042:1155:1156:1160:1260:1277:1311:1313:1314:1345:1391:1437:1515:1516:1518:1534:1541:1593:1594:1711:1730:1747:1766:1792:2075:2078:2198:2199:2377:2378:2380:2393:2525:2553:2567:2682:2685:2857:2859:2892:2933:2937:2939:2942:2945:2947:2951:2954:3022:3353:3636:3657:3865:3866:3867:3868:3869:3870:3871:3874:3934:3936:3938:3941:3944:4250:4321:4470:5007:6119: 6, 27 -- 6261:7679,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 6, 27 -- X-Spamcatcher-Explanation: 6, 27 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 6, 27 -- (Authenticated sender: michael-at-shaffer.net) 6, 27 -- by emd2-omf02.hostedemail.com (Postfix) with ESMTP 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 16:06:31 +0000 (UTC) 6, 27 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 27 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 27 -- Subject: Short course announcement: SEM-platform image analysis 6, 27 -- Date: Thu, 7 Feb 2008 12:43:59 -0330 6, 27 -- Message-ID: {000a01c869a4$745b9090$8d829986-at-CREAIT.MUN.CA} 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="US-ASCII" 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- X-Mailer: Microsoft Office Outlook 11 6, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 6, 27 -- Thread-Index: AchppHJoVzH9ijfXSOaB7eEctjC6KQ== ==============================End of - Headers==============================
Keith, thanks for a thorough and informative post! And Tom, have you actually been using a tablet already and now are considering an upgrade to Cintiq? Or you haven't use either yet?
Anyway, I have just one comment to add now: that our hand (even my clumsy one) seems to adjust very easily to any scaling when you trace with a Wacom pen on your tablet while looking at the screen. Seriously, I remember I was surprised at first. The hand learns immediately and has no trouble following the outline, even if the magnification on the screen is much higher. That means that bigger tablet may not be better. Think about how exactly you will be using it - setting on your desk in front of the monitor? or on your lap? on your knee sitting with your legs crossed? We found Wacom's second small size to be optimal. Bigger tablet can feel really bulky and harder to balance; besides, there are those buttons on the tablet's sides, and you won't be able to reach them as easy.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 5, 22 -- From vladislav_speransky-at-nih.gov Thu Feb 7 11:57:56 2008 5, 22 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17HvtHn002067 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 11:57:55 -0600 5, 22 -- X-IronPortListener: NIH_Relay 5, 22 -- X-SBRS: None 5, 22 -- X-IronPort-AV: E=Sophos;i="4.25,316,1199682000"; 5, 22 -- d="scan'208";a="300074426" 5, 22 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 22 -- by nihrelayxway3.hub.nih.gov with ESMTP; 07 Feb 2008 12:57:56 -0500 5, 22 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 22 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m17HvtTV21648775 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:57:55 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {4CE9FF05-2EF1-42AE-B116-D26BA3D66A52-at-nih.gov} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- Subject: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 12:57:37 -0500 5, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
I have not complained before about course presentations but I feel Michaels short course announcement goes beyond a course announcement and becomes a sales presentation on a product.
Those of us who earn our crust through microscopy and selling a service take great care not to step into a sales presentation situation; Michael I believe has overstepped the mark?
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
==============================Original Headers============================== 6, 28 -- From protrain-at-emcourses.com Thu Feb 7 12:02:24 2008 6, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17I2MV5006102 6, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:02:23 -0600 6, 28 -- Received: from 5ac8bf5d.bb.sky.com ([90.200.191.93]:3460 helo=HP6220) 6, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 6, 28 -- (envelope-from {protrain-at-emcourses.com} ) 6, 28 -- id 1JNB4l-0007D2-Tp by authid {f1f952b73f4f9e0d4f7dbb81b71a957d} with fixed_login 6, 28 -- for {microscopy-at-microscopy.com} ; Thu, 07 Feb 2008 20:02:21 +0200 6, 28 -- Message-ID: {001701c869b3$852e5dd0$0200a8c0-at-HP6220} 6, 28 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com} 6, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 6, 28 -- To: "American Society" {microscopy-at-microscopy.com} 6, 28 -- Subject: Michaels Short Course announcement 6, 28 -- Date: Thu, 7 Feb 2008 18:01:17 -0000 6, 28 -- Organization: Protrain 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; 6, 28 -- format=flowed; 6, 28 -- charset="Windows-1252"; 6, 28 -- reply-type=original 6, 28 -- Content-Transfer-Encoding: 7bit 6, 28 -- X-Priority: 3 6, 28 -- X-MSMail-Priority: Normal 6, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 6, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 6, 28 -- X-Scan-Signature: c3253b1522b319b8bde41fef99cb3562{17}} 6, 28 -- X-Trace: smtp01.dial-up.net 1JNB4l-0007D2-Tp cc945f1d1d9f618f8dfc8a00dac4f84b ==============================End of - Headers==============================
I haven't used any type of tablet before. I must admit that reading about them in some of the posts makes me wonder why I haven't. It is a less expensive option. But some of the other posts are clearly extolling the Cintiq which allows direct tracing on a near horizontal monitor. I have used a mouse to trace 1000's of objects and I am fairly adept but I know that I would have to be 10x faster tracing an object on a photograph or, in this case, the monitor. I was mostly looking to hear that this was as good as it seems on paper and it sounds like it should be. I am definitely going to bite the bullet and buy either the small or medium size monitor. I found the medium size one for 1900 and probably will go with that. I will eventually post a comment about my results once I get the new toy. I appreciate all the helpful comments. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: Thursday, February 07, 2008 11:59 AM To: Phillips, Thomas E.
Hello Tom and Keith,
Keith, thanks for a thorough and informative post! And Tom, have you actually been using a tablet already and now are considering an upgrade to Cintiq? Or you haven't use either yet?
Anyway, I have just one comment to add now: that our hand (even my clumsy one) seems to adjust very easily to any scaling when you trace with a Wacom pen on your tablet while looking at the screen. Seriously, I remember I was surprised at first. The hand learns immediately and has no trouble following the outline, even if the magnification on the screen is much higher. That means that bigger tablet may not be better. Think about how exactly you will be using it - setting on your desk in front of the monitor? or on your lap? on your knee sitting with your legs crossed? We found Wacom's second small size to be optimal. Bigger tablet can feel really bulky and harder to balance; besides, there are those buttons on the tablet's sides, and you won't be able to reach them as easy.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 5, 22 -- From vladislav_speransky-at-nih.gov Thu Feb 7 11:57:56 2008 5, 22 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17HvtHn002067 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 11:57:55 -0600 5, 22 -- X-IronPortListener: NIH_Relay 5, 22 -- X-SBRS: None 5, 22 -- X-IronPort-AV: E=Sophos;i="4.25,316,1199682000"; 5, 22 -- d="scan'208";a="300074426" 5, 22 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 22 -- by nihrelayxway3.hub.nih.gov with ESMTP; 07 Feb 2008 12:57:56 -0500 5, 22 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 22 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m17HvtTV21648775 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:57:55 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {4CE9FF05-2EF1-42AE-B116-D26BA3D66A52-at-nih.gov} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- Subject: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 12:57:37 -0500 5, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 26 -- From PhillipsT-at-missouri.edu Thu Feb 7 13:39:10 2008 16, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17JdAOi030895 16, 26 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 7 Feb 2008 13:39:10 -0600 16, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 26 -- Thu, 7 Feb 2008 13:39:09 -0600 16, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 16, 26 -- Content-class: urn:content-classes:message 16, 26 -- MIME-Version: 1.0 16, 26 -- Content-Type: text/plain; 16, 26 -- charset="us-ascii" 16, 26 -- Subject: RE: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Date: Thu, 7 Feb 2008 13:39:07 -0600 16, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD038C96E1-at-UM-XMAIL06.um.umsystem.edu} 16, 26 -- In-Reply-To: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- X-MS-Has-Attach: 16, 26 -- X-MS-TNEF-Correlator: 16, 26 -- Thread-Topic: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Thread-Index: AchpsyjSCQHx2QGeTue5OKjW/VH0WwADVTDg 16, 26 -- References: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 26 -- To: {vladislav_speransky-at-nih.gov} 16, 26 -- Cc: {Microscopy-at-msa.microscopy.com} 16, 26 -- X-OriginalArrivalTime: 07 Feb 2008 19:39:09.0951 (UTC) FILETIME=[1C0148F0:01C869C1] 16, 26 -- Content-Transfer-Encoding: 8bit 16, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m17JdAOi030895 ==============================End of - Headers==============================
We are attempting to repair a Wild M20 light microscope for a colleague. The fine focus knob is no longer independently moveable but is "fused" to the coarse focus. I believe the gearing mechanism and associated metal parts need to be cleaned of polymerized lubricant. I have disassembled the scope to gain access to the gearing but cannot figure out how to remove the focus knobs to expose the gearing. Does anyone know how this might be done? Thank you. -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
No I don't present my last book, I just need your help to solve a mysterious peak in EDX ;-) Here is the story: A colleague was analysing a piece of zeolite in SEM with EDX (Tescan SEM, Oxford Instr. EDX). The specimen was sputter-coated with carbon and the HT was 20 keV (W filament). I told her to try stick with a deadtime of about 20% and adjusted the parameters accordingly. At that moment we had the nice Al peak (1,49 keV), Si peak (1,74 keV) and a smaller but very clear Ca peak (3,69 keV). Happyness at its best. Then I left her to have a cup of coffee...eer I mean to study a very difficult case and when I was back she said she found some mercury in the sample! (which is absolutely unexpected even as contaminant). She had increased the spot size and had a deadtime of more than 70%!! And indeed she had a very tiny Hg peak at 2,19 keV! All others parameters being the same, when I decreased the spot size the Hg peak disappeared. This is obviously an artifact, but after reading the book from Goldstein, Newbury at al. I cannot find to which artifact belongs that Hg peak. Any thought about it?
Best regards,
Stephane
PS: I will summarize the answers I got from my question about the IGP pump replacement soon
____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Fri Feb 8 02:15:33 2008 7, 19 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m188FWUP013082 7, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 02:15:32 -0600 7, 19 -- Received: (qmail 83548 invoked by uid 60001); 8 Feb 2008 08:15:32 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 19 -- b=ZuHUvPzVvVcxTFeG/DZUKSUZzfXJBzbC2EligDPZuZSzGDp4Mb0sUIRO0Lh4loLr2QhuZxiokdnkAiLfSdv1Fel1wI3WHvn7WcOSPKBqJwjlPJfP3lelZHP6Engxx8ID1+6J9jh1paTTX1pQyPVYYUg/6OoA1RZjC+mYUb3WABM=; 7, 19 -- X-YMail-OSG: rwxGQEEVM1kny9k7VgIi2iKvuAvYQTepPc_QTIfgXqWgqlMCk3Rn8z0sIZUV_rHAAyGNJNFRGMtVSsxV9qKotYk4Z3Q.83iPMmNYIls6qgIfasJQUKU- 7, 19 -- Received: from [80.122.101.100] by web37410.mail.mud.yahoo.com via HTTP; Fri, 08 Feb 2008 00:15:32 PST 7, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 7, 19 -- Date: Fri, 8 Feb 2008 00:15:32 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: [Microscopy] The mercury mystery 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {345314.82936.qm-at-web37410.mail.mud.yahoo.com} ==============================End of - Headers==============================
I know it might sound silly, but why don't you have a go at making your own? You already have the tablet. http://www.bongofish.co.uk/wacom/wacom_pt1.html
I wasn't actually aware of the cintiq until the post on here, I've made do with a regular tablet. After seeing the cintiq kit that wacom sell I think it's superb, then I saw the price, and then I thought I'd bet I could make my own. A couple of searches and I found this website; many people have already done this with their own tablets and I'm about to have a bash too!
Hope this helps
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: 06 February 2008 16:34 To: a.a.evans-at-Bradford.ac.uk
I am thinking about buying a Wacom Cintiq monitor/tablet so that I can trace objects during on the screen for Photoshop operations (e.g., area measurements using the Fovea Pro toolkit). I would welcome comments from those using this approach about its ease and success rate. The 6 x 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any thoughts on the size of the tablet or the DTF vs. Cintiq models would also be useful. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
I have to say, having got so used to a mouse, I find a Wacom standard mouse pad A5 sized tablet and a stylus pen so intuitive as it is controlled just like the mouse (even to the same scale of movement), but with far better resolution - although I have to admit the little Wacom also meets my budget requirements rather well and I'm less fussy about an perfect trace (biological variation being rather greater than an exact outline). Plus MetaMorph can undo [backtrace] so easily.
With a light pen on a 15inch CRT you are moving your arm around a lot more (and back in the 1980/90s the screens were tough glass) - but I was impressed compared the basic 1980s ball mouse - although the image editing was hardware based back then not software, and so was lightening fast.
I wouldn't fancy a stylus on a modern soft fronted CRT screen - my touchscreen PDAs and video camera screens are looking pretty manky after a few years use with a stylus - as does my kids Nintendos, but they at least have a plastic sticky screen protector (that looks naff with bubbles everywhere). I find bending down to trace things a lot more fatiguing that looking horizontally at a vertical PC monitor. Plus the A5 Wacom just hot USB2 unplugs and goes in the drawer afterwards as desktop space is a premium these days. Also Wacom tablets survive a hot cup of coffee and sandwich crumbs with ease - and if they didn't they are also cheaper to replace (stylues pens excepted, they are always getting lost). I guess I just don't like tablet PCs really - handwriting is so last millennium - plus I want plenty of raw power i.e. an imaging workstation desktop [laptops need not apply].
I would be interested to get an update on the Cintiq if you get one - I'm sure a lot of others on the list-server would as well.
Thanks for an interesting post.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: 07 February 2008 19:52 To: kjmorris-at-well.ox.ac.uk
I haven't used any type of tablet before. I must admit that reading about them in some of the posts makes me wonder why I haven't. It is a less expensive option. But some of the other posts are clearly extolling the Cintiq which allows direct tracing on a near horizontal monitor. I have used a mouse to trace 1000's of objects and I am fairly adept but I know that I would have to be 10x faster tracing an object on a photograph or, in this case, the monitor. I was mostly looking to hear that this was as good as it seems on paper and it sounds like it should be. I am definitely going to bite the bullet and buy either the small or medium size monitor. I found the medium size one for 1900 and probably will go with that. I will eventually post a comment about my results once I get the new toy. I appreciate all the helpful comments. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: Thursday, February 07, 2008 11:59 AM To: Phillips, Thomas E.
Hello Tom and Keith,
Keith, thanks for a thorough and informative post! And Tom, have you actually been using a tablet already and now are considering an upgrade to Cintiq? Or you haven't use either yet?
Anyway, I have just one comment to add now: that our hand (even my clumsy one) seems to adjust very easily to any scaling when you trace with a Wacom pen on your tablet while looking at the screen. Seriously, I remember I was surprised at first. The hand learns immediately and has no trouble following the outline, even if the magnification on the screen is much higher. That means that bigger tablet may not be better. Think about how exactly you will be using it - setting on your desk in front of the monitor? or on your lap? on your knee sitting with your legs crossed? We found Wacom's second small size to be optimal. Bigger tablet can feel really bulky and harder to balance; besides, there are those buttons on the tablet's sides, and you won't be able to reach them as easy.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 5, 22 -- From vladislav_speransky-at-nih.gov Thu Feb 7 11:57:56 2008 5, 22 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17HvtHn002067 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 11:57:55 -0600 5, 22 -- X-IronPortListener: NIH_Relay 5, 22 -- X-SBRS: None 5, 22 -- X-IronPort-AV: E=Sophos;i="4.25,316,1199682000"; 5, 22 -- d="scan'208";a="300074426" 5, 22 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 22 -- by nihrelayxway3.hub.nih.gov with ESMTP; 07 Feb 2008 12:57:56 -0500 5, 22 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 22 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m17HvtTV21648775 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:57:55 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {4CE9FF05-2EF1-42AE-B116-D26BA3D66A52-at-nih.gov} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- Subject: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 12:57:37 -0500 5, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 26 -- From PhillipsT-at-missouri.edu Thu Feb 7 13:39:10 2008 16, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17JdAOi030895 16, 26 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 7 Feb 2008 13:39:10 -0600 16, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 26 -- Thu, 7 Feb 2008 13:39:09 -0600 16, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 16, 26 -- Content-class: urn:content-classes:message 16, 26 -- MIME-Version: 1.0 16, 26 -- Content-Type: text/plain; 16, 26 -- charset="us-ascii" 16, 26 -- Subject: RE: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Date: Thu, 7 Feb 2008 13:39:07 -0600 16, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD038C96E1-at-UM-XMAIL06.um.umsystem.edu} 16, 26 -- In-Reply-To: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- X-MS-Has-Attach: 16, 26 -- X-MS-TNEF-Correlator: 16, 26 -- Thread-Topic: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Thread-Index: AchpsyjSCQHx2QGeTue5OKjW/VH0WwADVTDg 16, 26 -- References: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 26 -- To: {vladislav_speransky-at-nih.gov} 16, 26 -- Cc: {Microscopy-at-msa.microscopy.com} 16, 26 -- X-OriginalArrivalTime: 07 Feb 2008 19:39:09.0951 (UTC) FILETIME=[1C0148F0:01C869C1] 16, 26 -- Content-Transfer-Encoding: 8bit 16, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m17JdAOi030895 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 22 -- From kjmorris-at-well.ox.ac.uk Fri Feb 8 03:41:41 2008 30, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 30, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m189fbU5008790 30, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 8 Feb 2008 03:41:38 -0600 30, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 30, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 30, 22 -- id 1JNPjk-00039Z-W4 30, 22 -- for Microscopy-at-Microscopy.Com; Fri, 08 Feb 2008 09:41:37 +0000 30, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 30, 22 -- To: {Microscopy-at-Microscopy.Com} 30, 22 -- References: {200802071952.m17Jq3sp010657-at-ns.microscopy.com} 30, 22 -- Subject: RE: [Microscopy] RE: RE: Wacom tablets/touch-screen monitors 30, 22 -- Date: Fri, 8 Feb 2008 09:41:57 -0000 30, 22 -- Message-ID: {000901c86a36$d8e45500$b22c4381-at-CytoWhizz} 30, 22 -- MIME-Version: 1.0 30, 22 -- Content-Type: text/plain; 30, 22 -- charset="us-ascii" 30, 22 -- Content-Transfer-Encoding: 7bit 30, 22 -- X-Mailer: Microsoft Office Outlook 11 30, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 30, 22 -- Thread-Index: AchpwulfBvyYUz37TFCmi3ahbZZK6AAbx/Yg 30, 22 -- In-Reply-To: {200802071952.m17Jq3sp010657-at-ns.microscopy.com} ==============================End of - Headers==============================
I am planning my 2008 year professional development right now, and I would like to take a short (but intensive) course in SEM imaging. I would like to learn how to bring out better images, as well as some sample preparation techniques for biological samples. I can get away for up to a week for a course, possibly with return visits at a later date for follow-up. If anyone has taken such a course, or offers one, please send your comments to me off-list.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Fri Feb 8 07:28:27 2008 3, 27 -- Received: from hs-out-2122.google.com (hs-out-0708.google.com [64.233.178.246]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18DSQ4r029395 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 07:28:27 -0600 3, 27 -- Received: by hs-out-2122.google.com with SMTP id 55so2231296hsc.2 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 08 Feb 2008 05:28:26 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=3pv8xHOR3W+r5uFAlFwkuUQ8MvP/js89aPiz8V3PLiU=; 3, 27 -- b=VOrT1pspi1ZLxxQDkzYBQs30PX7lT1NTJkkVRGDwj+HC9IMHBoyMrHSsCPEKf8Vza0hvB1FEG5H2ON8tQv5wyK9D6h+hamU+BxwTPTe7BGMz7iFQhlKQEWiykTcMyfDn+QxxQ2QIC6AlmnplOJff+3Sq8xLIOYHFEsGGIfhudIo= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=ZW0F8u9UadW4y29Hz08OGgkHM2IQN23l8N8PYOsoU6w5w3DvUvxHQfBko7TP0IskfHcsAKo8SS7ExJunpdO7ozMh+UlljEjYmZiW9S/njz+M7d03TSvywTmgQEBW6cay7mkkUMTLd9akQaIlVlN1WlwUQ+FlKodXoHwF2LkbKSo= 3, 27 -- Received: by 10.141.206.13 with SMTP id i13mr8481525rvq.211.1202477305311; 3, 27 -- Fri, 08 Feb 2008 05:28:25 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Fri, 8 Feb 2008 05:28:25 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20802080528g65072e1bw7f4a894d2155fb03-at-mail.gmail.com} 3, 27 -- Date: Fri, 8 Feb 2008 08:28:25 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: SEM Short Course recommendations. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
----- Original Message ---- X-from: "A.A.Evans-at-Bradford.ac.uk" {A.A.Evans-at-Bradford.ac.uk} To: nizets2-at-yahoo.com Sent: Friday, February 8, 2008 9:39:31 AM
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____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
==============================Original Headers============================== 35, 19 -- From nizets2-at-yahoo.com Fri Feb 8 07:43:16 2008 35, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 35, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m18DhGLl010046 35, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 07:43:16 -0600 35, 19 -- Received: (qmail 45074 invoked by uid 60001); 8 Feb 2008 13:43:16 -0000 35, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 35, 19 -- s=s1024; d=yahoo.com; 35, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 35, 19 -- b=yvzB1fhWrLCTEjVjxuDI1OZdOb+48GuKHPyVNzdq/cL6GR9ZpU3Xo1s7aeZ6VjU5CbvAuKJA0MQgmiGCijSAOhIQliOZ6/gayJjliC+oEunXPr0u488uj3WIkiWmFk2fQ6onfABRaWf+ko7Bcfp4G3WC2D3gm2n8M0OTj4cXBKE=; 35, 19 -- X-YMail-OSG: NfS_BpsVM1lBNjhWS3jzANGMW6FCX38RismqrT5rpvUTmZmZZAGZiNN1lLWwgeAP2fM1B_JshqjhnmZ4f90cJqKhbl2G56MPcE2pSfhdEiLV_g9PEjjcRpBG4fk1SNovBtTJgmw7S4MpTpqeZ04Nv.9d.VXuN2TbIypQTWlbFQ9c 35, 19 -- Received: from [80.121.2.186] by web37405.mail.mud.yahoo.com via HTTP; Fri, 08 Feb 2008 05:43:15 PST 35, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 35, 19 -- Date: Fri, 8 Feb 2008 05:43:15 -0800 (PST) 35, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 35, 19 -- Subject: Re: [Microscopy] Wacom tablets/touch-screen monitors 35, 19 -- To: microscopy-at-microscopy.com 35, 19 -- MIME-Version: 1.0 35, 19 -- Content-Type: text/plain; charset=us-ascii 35, 19 -- Message-ID: {118138.43483.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Lockheed Martin- KAPL, Inc. has an open position for a surface scientist (experienced in Auger / ESCA techniques). A brief description of the job is given below. NOTE: US Citizenship REQUIRED.
The complete job announcement (Req ID 66233BR) can be found at the Lockheed Martin career web site (LockheedMartin.com).
Jim McGee ************************************ James J. McGee Materials Engineer, Test Operations Lockheed Martin, KAPL, Inc. Mail Bin 149 PO Box 1072 Schenectady, NY 12301-1072
Required skills: MS degree in the physical sciences (e.g. materials science, chemistry, geology, solid state physics) or engineering, plus hands-on training and/or experience operating scanning Auger electron microscopy (SAM), Electron Spectroscopy for Chemical Analysis (ESCA), and Secondary Ion Mass Spectrometry (SIMS). Desired skills PhD degree in physical sciences with 5 years or more experience in surface and microanalysis (SAM, ESCA, and SIMS) and demonstrated problem solving experience in areas of metallurgy, alloy testing/development, solid inorganic materials, and/or ceramics. Previous experience and nuclear or radioactive materials, corrosion, metallurgy, and ceramics is a also desirable.
Specific Job Description: Characterize the surfaces of materials to support environmental testing and failure analysis of in-service components. Utilize field-emission Auger electron microscopy and imaging, ESCA spectroscopy and imaging, and SIMS. Execute data and image processing (e.g. Target-Factor Analysis (TFA, PCA), curve fitting, quantitative analysis, data assimilation, and reporting of results. Participate in multidisciplinary, collaborative investigations and team-oriented problem solving with other materials characterization specialists and materials scientists/engineers using associated analytical techniques (FEG-SEM, EBSD, EPMA, XRD, TEM, FTIR, Raman, FIB, TOF-SIMS).
The major duties will be the use of advanced surface analytical techniques to determine the microstructure and microchemistry of metals, alloys, and ceramic materials. Ensure instrumentation is properly maintained and in proper operating condition. Analyze and interpret data and communicate results to sponsoring groups by oral and written reports. Serve as part of research teams to design experiments that will elucidate structure-composition-property-processing relationships. Stay current in surface and microanalytical techniques and applications and propose/implement new capabilities or characterization tools.
==============================Original Headers============================== 14, 28 -- From mcgeejj-at-kapl.gov Fri Feb 8 08:22:47 2008 14, 28 -- Received: from mars.bias2000.bettis.gov (bettis.gov [65.170.176.212]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18EMkMN023867 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 08:22:46 -0600 14, 28 -- Received: from atlas.bias2000.bettis.gov (unverified) by mars.bias2000.bettis.gov 14, 28 -- (Clearswift SMTPRS 5.0.9) with SMTP id {T8504c37ef7ac1002681698-at-mars.bias2000.bettis.gov} for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 8 Feb 2008 09:22:51 -0500 14, 28 -- Received: from ibetpex02.bias2000.bettis.gov ([172.16.2.129]) by atlas.bias2000.bettis.gov with Microsoft SMTPSVC(6.0.3790.1830); 14, 28 -- Fri, 8 Feb 2008 09:22:50 -0500 14, 28 -- Content-class: urn:content-classes:message 14, 28 -- MIME-Version: 1.0 14, 28 -- Content-Type: text/plain; 14, 28 -- charset="us-ascii" 14, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 28 -- Subject: Open Position - Surface scientist for materials science applications 14, 28 -- Date: Fri, 8 Feb 2008 09:22:50 -0500 14, 28 -- Message-ID: {8807BA5B50FE0E40957843C1A36ACB83B36342-at-ibetpex02.bias2000.bettis.gov} 14, 28 -- In-reply-to: {8807BA5B50FE0E40957843C1A36ACB833D087D-at-ibetpex02.bias2000.bettis.gov} 14, 28 -- X-MS-Has-Attach: 14, 28 -- X-MS-TNEF-Correlator: 14, 28 -- Thread-Topic: Open Position - Surface scientist for materials science applications 14, 28 -- Thread-Index: Acg7M8tz2LXeTCaQQxK7z5idvCoucQvKUYTA 14, 28 -- References: {8807BA5B50FE0E40957843C1A36ACB833D087D-at-ibetpex02.bias2000.bettis.gov} 14, 28 -- From: "McGee, James" {mcgeejj-at-kapl.gov} 14, 28 -- To: {microscopy-at-microscopy.com} 14, 28 -- X-OriginalArrivalTime: 08 Feb 2008 14:22:50.0730 (UTC) FILETIME=[15EBBCA0:01C86A5E] 14, 28 -- Content-Transfer-Encoding: 8bit 14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m18EMkMN023867 ==============================End of - Headers==============================
The math would fit for the escape of Al x-rays during the detection of Ca (3.69 - 1.49). I wouldn't ordinarily expect that you would detect that sort of an escape peak, but at 70 dead time, who knows...? When your colleague cranked the dead time, did you also detect a new peak at 1.95, corresponding to Ca minus Si? If your mystery peak is an escape peak, I would think you would see this one as well.
Yours, Matt
Matthew Stephenson Impact Analytical/MMI 1910 W. Saint Andrews Rd. Midland, MI 48640 (989)832-5555 X506 stephenson-at-impactanalytical.com Visit Impact Analytical in Booth # 3305 at PittCon March 3rd - 7th
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Friday, February 08, 2008 3:23 AM To: stephenson-at-impactanalytical.com
Hi all!
No I don't present my last book, I just need your help to solve a mysterious peak in EDX ;-) Here is the story: A colleague was analysing a piece of zeolite in SEM with EDX (Tescan SEM, Oxford Instr. EDX). The specimen was sputter-coated with carbon and the HT was 20 keV (W filament). I told her to try stick with a deadtime of about 20% and adjusted the parameters accordingly. At that moment we had the nice Al peak (1,49 keV), Si peak (1,74 keV) and a smaller but very clear Ca peak (3,69 keV). Happyness at its best. Then I left her to have a cup of coffee...eer I mean to study a very difficult case and when I was back she said she found some mercury in the sample! (which is absolutely unexpected even as contaminant). She had increased the spot size and had a deadtime of more than 70%!! And indeed she had a very tiny Hg peak at 2,19 keV! All others parameters being the same, when I decreased the spot size the Hg peak disappeared. This is obviously an artifact, but after reading the book from Goldstein, Newbury at al. I cannot find to which artifact belongs that Hg peak. Any thought about it?
Best regards,
Stephane
PS: I will summarize the answers I got from my question about the IGP pump replacement soon
____________________________________________________________________________ ________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Fri Feb 8 02:15:33 2008 7, 19 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m188FWUP013082 7, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 02:15:32 -0600 7, 19 -- Received: (qmail 83548 invoked by uid 60001); 8 Feb 2008 08:15:32 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Ty pe:Message-ID; 7, 19 -- b=ZuHUvPzVvVcxTFeG/DZUKSUZzfXJBzbC2EligDPZuZSzGDp4Mb0sUIRO0Lh4loLr2QhuZxiokd nkAiLfSdv1Fel1wI3WHvn7WcOSPKBqJwjlPJfP3lelZHP6Engxx8ID1+6J9jh1paTTX1pQyPVYYU g/6OoA1RZjC+mYUb3WABM=; 7, 19 -- X-YMail-OSG: rwxGQEEVM1kny9k7VgIi2iKvuAvYQTepPc_QTIfgXqWgqlMCk3Rn8z0sIZUV_rHAAyGNJNFRGMtV SsxV9qKotYk4Z3Q.83iPMmNYIls6qgIfasJQUKU- 7, 19 -- Received: from [80.122.101.100] by web37410.mail.mud.yahoo.com via HTTP; Fri, 08 Feb 2008 00:15:32 PST 7, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 7, 19 -- Date: Fri, 8 Feb 2008 00:15:32 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: [Microscopy] The mercury mystery 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {345314.82936.qm-at-web37410.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 16, 26 -- From stephenson-at-impactanalytical.com Fri Feb 8 08:56:16 2008 16, 26 -- Received: from sa1.mitcon.org (sa1.mitcon.org [192.251.56.4]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18EuGON005155 16, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 08:56:16 -0600 16, 26 -- Received: from localhost (localhost [127.0.0.1]) 16, 26 -- by sa1.mitcon.org (Postfix) with ESMTP id 71D8B58967 16, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 09:56:16 -0500 (EST) 16, 26 -- X-Virus-Scanned: by amavisd-new-2.5.2 (20070627) (Debian) at mitcon.org 16, 26 -- Received: from sa1.mitcon.org ([127.0.0.1]) 16, 26 -- by localhost (sa1.mitcon.org [127.0.0.1]) (amavisd-new, port 10024) 16, 26 -- with ESMTP id yhkowhGbtSV5 for {microscopy-at-microscopy.com} ; 16, 26 -- Fri, 8 Feb 2008 09:56:14 -0500 (EST) 16, 26 -- X-Greylist: whitelisted by SQLgrey-1.6.8 16, 26 -- Received: from mitconexch.mitcon.org (mitconexch.mitcon.org [10.10.10.2]) 16, 26 -- by sa1.mitcon.org (Postfix) with ESMTP id 862E858964 16, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 09:56:14 -0500 (EST) 16, 26 -- Received: by mitconexch.mitcon.org with Internet Mail Service (5.5.2657.72) 16, 26 -- id {1J7CQV8L} ; Fri, 8 Feb 2008 09:56:14 -0500 16, 26 -- Message-ID: {089C7709BE9235448E3622C6F38D828F06C6959C-at-mitconexch.mitcon.org} 16, 26 -- From: stephenson-at-impactanalytical.com 16, 26 -- To: microscopy-at-microscopy.com 16, 26 -- Subject: RE: [Microscopy] The mercury mystery 16, 26 -- Date: Fri, 8 Feb 2008 09:56:10 -0500 16, 26 -- MIME-Version: 1.0 16, 26 -- X-Mailer: Internet Mail Service (5.5.2657.72) 16, 26 -- Content-Type: text/plain ==============================End of - Headers==============================
Good Morning: I have a potential project to compare the mechanical properties of collagen in the kneecap with its structure and arrangement. First I'm not sure this is a TEM project as another type of microscopy might be better. Secondly I assume I would have to embed and section the patella and could really use some help on the "how to". If anyone has experience working with this material I would be pleased to get some advice. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Fri Feb 8 09:28:31 2008 5, 26 -- Received: from dargo.cs.uoguelph.ca (dargo.cs.uoguelph.ca [131.104.94.197]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18FSUqi018813 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 09:28:30 -0600 5, 26 -- Received: from aragorn.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 5, 26 -- by dargo.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m18FSNCg003666 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 10:28:23 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by aragorn.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m18FSNsu011443 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 10:28:23 -0500 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Fri, 08 Feb 2008 10:28:23 -0500 5, 26 -- Message-ID: {20080208102823.yk0o50vlcs4sko48-at-webmail.uoguelph.ca} 5, 26 -- Date: Fri, 08 Feb 2008 10:28:23 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Collagen in the Patella 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.197 ==============================End of - Headers==============================
Dear listers, Below please find a description of a postdoctoral opportunity at Oak Ridge National Laboratory. Feel free to post or forward to any qualified candidates.
*Combined Scanning Transmission and Scanning Probe Microscopy of Ferroelectrics*
*Materials Science and Technology Division *
*Oak Ridge National Laboratory *
*Oak Ridge, Tennessee *
*Project Description: *
The Materials Science and Technology Division at Oak Ridge National Laboratory (ORNL) is seeking a candidate to fill a postdoctoral position in the field of combined scanning transmission electron microscopy and scanning probe microscopy of ferroelectric thin films. The position is available immediately. This program takes advantage of ORNL’s suite of advanced electron microscopes, including 4 aberration-corrected instruments, as well as recently acquired (S)TEM/STM and (S)TEM/AFM capabilities.
The successful applicant must demonstrate experience in electron microscope operation, preferably FEI microscopes, as well as skills in analysis and interpretation of microscopic and spectroscopic data. This position provides an opportunity to join an experienced team working in a highly collaborative environment on the projects ranging from heterogeneous catalysis to oxide materials to semiconductors. Close interactions with the scanning probe microscopy program at ORNL’s Center for Nanophase Materials Sciences (CNMS) are anticipated.
*Qualifications: PhD degree required *
This position requires a Ph.D. in Materials Science, Physics, or related field, with an emphasis on advanced TEM or STEM. Knowledge of oxide crystal chemistry is a plus. Excellent oral and written communication skills are required, and presentations and publication of scientific results in peer-reviewed journals are expected. The applicant must have the ability to work in a team and interact effectively with a broad range of colleagues. Applicants cannot have received the most recent degree more than five years prior to the date of application and must complete all degree requirements before starting their appointment.
*How to Apply: *
Qualified applicants may apply online at https://www2.orau.gov/ORNL_POST/ .
All applicants will need to register before they can begin the online application. For complete instructions, on how to apply, please see the instructions at
http://www.orau.gov/orise/edu/ornl/ornl-pdpm/application.htm . When applying for this position, please reference the position title and number ORNL08-23-MSTD). Questions regarding the position can be directed to Drs. Albina Y. Borisevich, albinab-at-ornl.gov {mailto:albinab-at-ornl.gov} , Stephen J. Pennycook, pennycooksj-at-ornl.gov {mailto:pennycooksj-at-ornl.gov} , and Sergei V. Kalinin, sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov} . Applications will be accepted until the position is filled. This appointment is offered through the ORNL Postgraduate Research Participation Program and is administered by the Oak Ridge Institute for Science and Education (ORISE). The program is open to all qualified U.S. and non-U.S. citizens without regard to race, color, age, religion, sex, national origin, physical or mental disability, or status as a Vietnam-era veteran or disabled veteran.
-- -- Albina Y. Borisevich R&D Associate Electron Microscopy Group Oak Ridge National Laboratory Materials Science and Technology Division PO Box 2008 Oak Ridge TN 37831-6031
http://stem.ornl.gov/
For express mail add: 1 Bethel Valley Road phone: (865) 576-4060 fax: (865) 574-4143
==============================Original Headers============================== 17, 25 -- From albinab-at-ornl.gov Fri Feb 8 14:48:18 2008 17, 25 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18KmIrJ012567 17, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 14:48:18 -0600 17, 25 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 17, 25 -- by emroute1.ornl.gov (PMDF V6.3-x14 #31501) 17, 25 -- with ESMTP id {0JVX0052CTSHE9-at-emroute1.ornl.gov} for 17, 25 -- microscopy-at-microscopy.com; Fri, 08 Feb 2008 15:48:18 -0500 (EST) 17, 25 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 17, 25 -- (PMDF V6.3-x14 #31501) id {0JVX00601TSH6H-at-emroute1.ornl.gov} for 17, 25 -- microscopy-at-microscopy.com; Fri, 08 Feb 2008 15:48:17 -0500 (EST) 17, 25 -- Received: from [160.91.48.156] (simulations1.ornl.gov [160.91.48.156]) 17, 25 -- by emroute1.ornl.gov (PMDF V6.3-x14 #31501) 17, 25 -- with ESMTPSA id {0JVX0017RTSHY6-at-emroute1.ornl.gov} for 17, 25 -- microscopy-at-microscopy.com; Fri, 08 Feb 2008 15:48:17 -0500 (EST) 17, 25 -- Date: Fri, 08 Feb 2008 15:48:17 -0500 17, 25 -- From: Albina Borisevich {albinab-at-ornl.gov} 17, 25 -- Subject: Postdoctoral position in Combined Scanning Transmission and Scanning 17, 25 -- Probe Microscopy of Ferroelectrics 17, 25 -- To: microscopy-at-microscopy.com 17, 25 -- Message-id: {47ACC011.9010404-at-ornl.gov} 17, 25 -- MIME-version: 1.0 17, 25 -- Content-type: text/plain; charset=UTF-8; format=flowed 17, 25 -- Content-transfer-encoding: 8BIT 17, 25 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) ==============================End of - Headers==============================
I have gone on line today to reserve my room for M&M 2008 and I chose the Double Tree Hotel. The rate for two was $125 instead of $119 as published on the Microscopy web site (I challenged this). There were no rooms available for Wed. and Thurs. at the discounted meeting rate. I inquired about non-discount rooms, not wishing to pack up and move and they were available at $165, if I remember correctly. Luckily the thrifty little voice in my head spoke up and I was able to get a AAA discount and the cost was $151 and $143.
The message from MSA was correct about the rooms going quickly!
Pat Connelly
==============================Original Headers============================== 5, 21 -- From connellyps-at-nhlbi.nih.gov Fri Feb 8 16:50:41 2008 5, 21 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18Mof2X029643 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 16:50:41 -0600 5, 21 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 5, 21 -- Fri, 8 Feb 2008 17:50:36 -0500 5, 21 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.167]) with Microsoft Exchange Server HTTP-DAV ; 5, 21 -- Fri, 8 Feb 2008 22:50:36 +0000 5, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 5, 21 -- Date: Fri, 08 Feb 2008 17:47:51 -0500 5, 21 -- Subject: Hotel for M&M 5, 21 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 5, 21 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 21 -- Message-ID: {C3D24647.1531%connellyps-at-nhlbi.nih.gov} 5, 21 -- Thread-Topic: Hotel for M&M 5, 21 -- Thread-Index: AchqpKJK4PmbVtaXEdyvlAANk2Yv1A== 5, 21 -- Mime-version: 1.0 5, 21 -- Content-type: text/plain; 5, 21 -- charset="US-ASCII" 5, 21 -- Content-transfer-encoding: 7bit 5, 21 -- X-OriginalArrivalTime: 08 Feb 2008 22:50:36.0270 (UTC) FILETIME=[04CC38E0:01C86AA5] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both friess-at-limedion.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: friess-at-limedion.de Name: Frank Frieþ
Organization: LMI
Title-Subject: [Filtered] 6300F/6400F/6600F
Question: Hi,
is there someone here who is running a JEOL 6xxxF Series with the serial (RS232) interface activated ? If it can be read (and maybe even be controled) some parameters like magnification, voltage etc. by a connected computer system (e.g. EDS) this would be an indicater that the serial is working. (as I found out the interface was often not activated when the devices were sold). I would be happy to find someone with a working RS232.
There are a few basic FA books on polymers out there. It seems that no one book covers the topic sufficiently to be "the" reference. The book you now have and the Engel book previously recommended are good. My other favorites: Failure of Plastics and Rubber Products by David Wright Plastics Failure Analysis and Prevention, John Moalli (Editor) Plastics Failure Guide by Myer Ezrin
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 3, 24 -- From hanke-at-mee-inc.com Fri Feb 8 18:05:11 2008 3, 24 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1905BLu024651 3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 18:05:11 -0600 3, 24 -- Received: (qmail 27771 invoked by uid 508); 8 Feb 2008 18:05:11 -0600 3, 24 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 3, 24 -- (clamdscan: 0.87/5723. spamassassin: 3.0.2. perlscan: 1.24-st-qms. 3, 24 -- Clear:RC:1(216.14.180.82):. 3, 24 -- Processed in 0.050229 secs); 09 Feb 2008 00:05:11 -0000 3, 24 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 3, 24 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.050229 secs Process 27764) 3, 24 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 3, 24 -- by mail.namisolutions.com with SMTP; 8 Feb 2008 18:05:11 -0600 3, 24 -- Message-ID: {47ACEE32.9080100-at-mee-inc.com} 3, 24 -- Date: Fri, 08 Feb 2008 18:05:06 -0600 3, 24 -- From: Larry Hanke {hanke-at-mee-inc.com} 3, 24 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 3, 24 -- MIME-Version: 1.0 3, 24 -- To: yuhong.wu-at-solvay.com, microscopy-at-microscopy.com 3, 24 -- Subject: Re: [Microscopy] viaWWW: Polymer Failure Analysis book 3, 24 -- References: {200802060523.m165NqpL004269-at-ns.microscopy.com} 3, 24 -- In-Reply-To: {200802060523.m165NqpL004269-at-ns.microscopy.com} 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, has someone out there a procedure for opening up the column of a 1997 EM 208S at the specimen stage to get a jammed prep-holder out again? Anything special to take care of?
Best regards, Stefan
Aktuelles: "Die Ästhetik des Unsichtbaren" - Pflanzenoberflächen unter dem Elektronenmikroskop Siehe www.elektronenmikroskopie.info/ausstelllungen/wuerzburg Mein Jahreskalender 2008 zum herunterladen und selbst ausdrucken im Format DIN A3: www.quantifoil.com/calendar_2008.pdf (38 MB) Falls Sie Bilder daraus kommerziell verwenden möchten fragen Sie mich bitte vorher...
Here is a summary of the different answers and finally what I think is the right one. I want to thank everybody for their help, I received lots of answers, almost all different but all very interesting.
1) Hg may be an escape peak of Ca. However in my book, and as someone stated, this does not really fit well. Ca (3,69) -1,74 (following my book) is not very near to 2,19 (Hg peak)
2) It is not a contamination, since this Hg peaks appears only at (too) high a deadtime. It clearly indicates an artifact.
3) One comment stated "It's the Ca peak (3.69) exciting the Al peak (1.49) leaving a 2.20 kV X-ray to exit the sample". I don't understand it!! (sorry)
3) The most reasonable answer is a pileup peak of O+Si. The 2 peaks are the 2 highest in the spectrum and their addition perfectly fits to the Hg peak. The person who proposed that solution seems to be experienced with this type of material and problem and this pileup does not seem so uncommon for high deadtimes.
I hope this can help others.
Best regards, Stephane
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==============================Original Headers============================== 10, 19 -- From nizets2-at-yahoo.com Tue Feb 12 05:01:35 2008 10, 19 -- Received: from web37409.mail.mud.yahoo.com (web37409.mail.mud.yahoo.com [209.191.91.141]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1CB1Yvo000426 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 05:01:34 -0600 10, 19 -- Received: (qmail 79686 invoked by uid 60001); 12 Feb 2008 11:01:34 -0000 10, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 19 -- s=s1024; d=yahoo.com; 10, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 19 -- b=sdb/1OOan8CsPMUHncrtuhqj8zYyHcAPSmDXyPjCm5lwwbhnNx79T6WLyjUmI4F01WlChpm93S2hkggQhuG9talchtgqOOvYPOxRT4N4gRLoJKr9/l8NyaKfyhBG/LlwjtJ1Rj78XiNuFzzLCc2uZdP+yOfNowahfwVsp7t/HR4=; 10, 19 -- X-YMail-OSG: 4Z_mM5gVM1myonBevvr8LbyC8CbETsr5OQopq60o42SdJcLD6.Mqkjnc0M9pMxFE848O5mOgCs5Q8mH097Rm8zB50PCE19nCOPx99SgYEPr6b.AzPzY- 10, 19 -- Received: from [80.122.101.100] by web37409.mail.mud.yahoo.com via HTTP; Tue, 12 Feb 2008 03:01:34 PST 10, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 10, 19 -- Date: Tue, 12 Feb 2008 03:01:34 -0800 (PST) 10, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 10, 19 -- Subject: the mercury mystery solved!! 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: text/plain; charset=us-ascii 10, 19 -- Message-ID: {494167.73968.qm-at-web37409.mail.mud.yahoo.com} ==============================End of - Headers==============================
I concur with number 3. I strongly suspected O+Si was the explanation. Since I hadn't seen it suggest as an answer, I threw some SiO2 into our older SEM yesterday afternoon and collected spectra at 20%, 30%, and 60% deadtime. I pasted the spectra and analytical results into a file which you may find here (ftp://www.marl.iastate.edu/General/SiO2_with_Hg.pdf ). You can see the "Hg" peak increase with increasing deadtime. You can also see the O+O and Si+Si sum peaks, but the "Hg" O+Si sum peak seems to be the highest in this case.
The analytical results quantify the effect. The Hg mass fraction is reported as 1.3, 1.7 and 6.8%. I did not push the exercise to lower deadtimes. I also did not try tweaking our old Kevex pulse processor to improve its pile-up rejection (for the record, the Kevex feeds an IXRF Systems box). I should repeat this experiment on our newer EDS system and see if it performs any better.
The moral of the story is that sum peaks are one of the main reasons that deadtime needs to be limited. Also, just because you can't see a peak (e.g., O or C) doesn't mean in can't pile up and appear in the spectrum.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, February 12, 2008 5:03 AM To: wesaia-at-iastate.edu
Dear list,
Here is a summary of the different answers and finally what I think is the right one. I want to thank everybody for their help, I received lots of answers, almost all different but all very interesting.
1) Hg may be an escape peak of Ca. However in my book, and as someone stated, this does not really fit well. Ca (3,69) -1,74 (following my book) is not very near to 2,19 (Hg peak)
2) It is not a contamination, since this Hg peaks appears only at (too) high a deadtime. It clearly indicates an artifact.
3) One comment stated "It's the Ca peak (3.69) exciting the Al peak (1.49) leaving a 2.20 kV X-ray to exit the sample". I don't understand it!! (sorry)
3) The most reasonable answer is a pileup peak of O+Si. The 2 peaks are the 2 highest in the spectrum and their addition perfectly fits to the Hg peak. The person who proposed that solution seems to be experienced with this type of material and problem and this pileup does not seem so uncommon for high deadtimes.
I hope this can help others.
Best regards, Stephane
==============================Original Headers============================== 19, 37 -- From wesaia-at-iastate.edu Tue Feb 12 09:15:23 2008 19, 37 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 19, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1CFFNn1023916 19, 37 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 12 Feb 2008 09:15:23 -0600 19, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 19, 37 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m1CFFNv5018276; 19, 37 -- Tue, 12 Feb 2008 09:15:23 -0600 19, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 19, 37 -- id 147f_50b9343c_d97d_11dc_9726_00137253420a; 19, 37 -- Tue, 12 Feb 2008 09:15:16 -0600 19, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 19, 37 -- by despam-10.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m1CFFGnv027152; 19, 37 -- Tue, 12 Feb 2008 09:15:21 -0600 19, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 19, 37 -- Tue, 12 Feb 2008 09:15:09 -0600 19, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 37 -- Content-class: urn:content-classes:message 19, 37 -- MIME-Version: 1.0 19, 37 -- Content-Type: text/plain; 19, 37 -- charset="us-ascii" 19, 37 -- Subject: RE: [Microscopy] the mercury mystery solved!! 19, 37 -- Date: Tue, 12 Feb 2008 09:15:20 -0600 19, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D727028845D3-at-maire.eng.iastate.edu} 19, 37 -- In-Reply-To: {200802121102.m1CB2rwt001699-at-ns.microscopy.com} 19, 37 -- X-MS-Has-Attach: 19, 37 -- X-MS-TNEF-Correlator: 19, 37 -- Thread-Topic: [Microscopy] the mercury mystery solved!! 19, 37 -- Thread-Index: AchtZteWLKGGWczDQ0C7FkvLJ5KnvQAHM+qA 19, 37 -- References: {200802121102.m1CB2rwt001699-at-ns.microscopy.com} 19, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 19, 37 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 19, 37 -- Cc: {nizets2-at-yahoo.com} 19, 37 -- X-OriginalArrivalTime: 12 Feb 2008 15:15:09.0402 (UTC) FILETIME=[0E5E13A0:01C86D8A] 19, 37 -- X-PMX-Version: 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2008.2.12.70212 19, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='ECARD_KNOWN_DOMAINS 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_SUBJ_9 0' 19, 37 -- Content-Transfer-Encoding: 8bit 19, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1CFFNn1023916 ==============================End of - Headers==============================
You can find a fairly detailed description of various vacuum greases in Sect. 10.12 (p.458) of my book 'Vacuum Methods in Electron Microscopy' I would recommend one of the perfluorinated polyphenyl ether greases Brayco 803 or Krytox LVP for most applications in electron microscopy. These greases are excellent lubricants, and are chemically inert (and so should not cause any corrosive reactions with metal parts), and have vapor pressures in the 10^-10 Torr range. However, I have recently learned of a grease called Santovac-5GB that is based on the polyphenyl ether vacuum fluid Santovac-5 which also has excellent properties (vapor pressure of 4 x 10^-10 Torr, stable up to 450 C, excellent as a lubricant, no tendency to spread or bleed, etc.) and which might be equally good. Some people prefer not to use the perfluorinated compounds in their instruments, and so this might be a good alternative choice. You can find a detailed description of both types of grease in the SPI online catalog. The perfluorinated polyphenyl ether diffusion pump fluids Fomblin Y-25/9 and Krytox 1625 are also very good lubricants, particularly for O-rings on rotating shafts. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Tue Feb 12 14:20:07 2008 1, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1CKK7bA015436 1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 14:20:07 -0600 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY hellskitchen.mr.itd.umich.edu ID 47B1FF75.490E8.32703 ; 1, 14 -- 12 Feb 2008 15:20:05 -0500 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240800c3d7a6c6b3b5-at-[141.212.131.221]} 1, 14 -- Date: Tue, 12 Feb 2008 15:20:02 -0500 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: Vacuum Grease 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Basically, the lifetime of ion getter pumps is determined by the amount of titanium available in their cathodes for producing the gettering action that is the basis of the pumps' operation, and also by the pressure range at which the pumps are operated. This matter is discussed in some detail in Sect. 7.1.8 (p 294) of my book Vacuum Methods in Electron Microscopy. As noted there, most manufacturers rate pump life on the basis of continuous operation at a pressure of 10^-6 Torr. Typical values are 45,000 to 50,000 hours (5 to 6 years). However, if the pump operates at a pressure of 10^-7 Torr (not unusual for pumps on the electron guns of modern instruments) then the operating life would be ten times as long, or of the order of 50 years. The development of "flakes", whiskers, and contamination on the insulators can shorten pump life, as mentioned by others. All of these factors are discussed in the above reference.. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Tue Feb 12 14:40:04 2008 1, 14 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1CKe4eo028454 1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 14:40:04 -0600 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY skycaptain.mr.itd.umich.edu ID 47B20422.33DAB.3474 ; 1, 14 -- 12 Feb 2008 15:40:02 -0500 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240801c3d7b048edf3-at-[141.212.131.221]} 1, 14 -- Date: Tue, 12 Feb 2008 15:40:01 -0500 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: Ion pump life 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wesley-smith-at-ukzn.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wesley-smith-at-ukzn.ac.za Name: James Wesley-Smith
Organization: University of KwaZulu-Natal, Durban, South Africa
Question: Dear colleagues If anyone out there has a Polaron E6300 vacuum evaporator, I would really appreciate if you could send me a scanned copy of the circuit diagramn needed for some troubleshooting.
Many thanks in advance!
James
Dr James Wesley-Smith Electron Microscope Unit University of KZN, Westville Campus Private Bag X54001 Durban 4000 South Africa
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-sheffield.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-sheffield.ac.uk Name: Zoe
Title-Subject: [Filtered] carbon contamination while doing EELS
Question: I found bad carbon contamination while put Cr3C2 samples in a 2010 FEGTEM for EELS analysis. They were thin film cross sections made by conventional sandwich method by Epoxy glue, and Gatan PIPS ion milling. I have examined three samples made by the same route, two turned out contaminated easily, but one seemed not much affected. The samples were subjected to selected area EELS in diffraction mode and STEM ADF imaiging. Carbon accumulated at the edge of the selected area circle. Once the beam was focused, carbon K-edge was enchanced in the EELS acquired. Amorphous carbon in EEL spectra stops me from studying the real carbon bonding from the Cr3C2 phase.
My questions are: (1)Why were some samples contaminated, other not much? (2)What could be the most possible contamination sources? (3)How can I minimise the contamination both during sample preperation and TEM examination? (3)How reliable is the carbon data acquired?
I suspect that may be breaking down your carbide to form your amorphous carbon when you are focusing the beam onto the sample.
I had a sample of a pulsed laser deposited diamond-like carbon film. You can see an image of this film on our website in the application note #59 (http://www.southbaytech.com/appnotes/59%20EELS%20of%20PLD%20DLC.pdf) that was prepared by MicroCleaving(TM). The film was amorphous, but there are alternating light and dark bands in the DLC film. EELS showed that the darker bands were SP3 bonding while the light bands were SP2/SP3 or more graphitic. We only had a parallel EELS on a CM200FEG. When the spot was focused down in order to isolate a dark band, the earliest EELS spectra showed no Pi-star peak, but almost instantly, started growing one and a spot formed. If the beam was spread out, no contamination was seen. It was only in the highly focused beam that we got it and in the first instant, you could clearly see that the dark bands had no SP2 bonding. I always wanted to revisit these samples with a GIF to examine the sample without the conversion, similar to what Jim Bentley did with his GIF and carbon films.
Another question for you if you think that it is actual contamination is have you plasma cleaned your sample? If you always plasma clean your samples before putting them in the microscope and other samples do not contaminate in your microscope, then the source is not your sample and not your microscope, but is probably the mechanism that I described above.
For a source of contamination, check the O-rings and lubrication on your whisper lock on the PIPS. The same Ar gas that is used for the guns is used for the lift assembly. A colleague of mine has demonstrated conclusively that the Ar gas was contaminated by the lubrication from the lift mechanism in the PIPS. We strongly advise our customers who have either a Gentle Mill(TM) or an IV3/4 equipped with a low energy gun to not share the gas line with a PIPS instrument for that reason. Your difference in contamination could be the amount of time the samples were left in the PIPS. However, if you plasma clean your samples, you will eliminate this source or any other external source of contamination on your samples.
Disclaimer: South Bay Technology manufactures and sells the MicroCleave(TM) Kit and the PC-2000 Plasma Cleaner. We also distribute the Technoorg-Linda Gentle Mill(TM) and IV3/4 ion mills in the United States.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: z.zhou-at-sheffield.ac.uk [mailto:z.zhou-at-sheffield.ac.uk] Sent: Tuesday, February 12, 2008 3:55 PM To: Walck-at-SouthBayTech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-sheffield.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-sheffield.ac.uk Name: Zoe
Title-Subject: [Filtered] carbon contamination while doing EELS
Question: I found bad carbon contamination while put Cr3C2 samples in a 2010 FEGTEM for EELS analysis. They were thin film cross sections made by conventional sandwich method by Epoxy glue, and Gatan PIPS ion milling. I have examined three samples made by the same route, two turned out contaminated easily, but one seemed not much affected. The samples were subjected to selected area EELS in diffraction mode and STEM ADF imaiging. Carbon accumulated at the edge of the selected area circle. Once the beam was focused, carbon K-edge was enchanced in the EELS acquired. Amorphous carbon in EEL spectra stops me from studying the real carbon bonding from the Cr3C2 phase.
My questions are: (1)Why were some samples contaminated, other not much? (2)What could be the most possible contamination sources? (3)How can I minimise the contamination both during sample preperation and TEM examination? (3)How reliable is the carbon data acquired?
I have had the same experience with many different samples. The contamination is one the sample and why or how some are cleaner than others seems to be a mystery. However it must be correlated to cleanliness of glues, acetone, and alcohols used during preparation. Try to keep them clean, generally not sharing with others helps. After a soak, follow with a rinse of fresh liquid before drying with clean air.
If possible plasma clean your specimens before putting them in the scope. Any plasma cleaning will help, but I have found the H2/O2 recipes superior to Ar or Ar/O2.
I am assuming that you are using the liquid N2 cold trap on the 2010. Have is cold before inserting the sample.
If you have some old liquid freon in the lab, you can use it to rinse the sample before inserting in the TEM. This is a great help, but not for the environment.
If you do not have freon, or access to a plasma cleaner, and must work with the samples as they are, then you could try "locking the carbon in place on the surface of the sample". This might be done by using a large spot size beam spread over a relatively large area, to bake the carbon in place. I am not sure how well this works or for how long you have to let the beam set to be useful, but others will probably offer advice on this approach.
Good luck. You are not alone in your plight.
Roseann
Roseann Csencsits, PhD Donner TEM Facility Manager Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Feb 12, 2008, at 4:01 PM, z.zhou-at-sheffield.ac.uk wrote:
} } } Email: z.zhou-at-sheffield.ac.uk } Name: Zoe } } Title-Subject: [Filtered] carbon contamination while doing EELS } } Question: I found bad carbon contamination while put Cr3C2 samples in } a 2010 FEGTEM for EELS analysis. They were thin film cross sections } made by conventional sandwich method by Epoxy glue, and Gatan PIPS } ion milling. I have examined three samples made by the same route, } two turned out contaminated easily, but one seemed not much affected. } The samples were subjected to selected area EELS in diffraction mode } and STEM ADF imaiging. Carbon accumulated at the edge of the selected } area circle. Once the beam was focused, carbon K-edge was enchanced } in the EELS acquired. Amorphous carbon in EEL spectra stops me from } studying the real carbon bonding from the Cr3C2 phase. } } My questions are: } (1)Why were some samples contaminated, other not much? } (2)What could be the most possible contamination sources? } (3)How can I minimise the contamination both during sample } preperation and TEM examination? } (3)How reliable is the carbon data acquired? } } Zoe
==============================Original Headers============================== 17, 26 -- From RCsencsits-at-lbl.gov Tue Feb 12 23:25:04 2008 17, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1D5P2Dk014947 17, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 23:25:03 -0600 17, 26 -- X-Ironport-SBRS: 4.7 17, 26 -- X-IronPort-Anti-Spam-Filtered: true 17, 26 -- X-IronPort-Anti-Spam-Result: AgAAAEIOskeAAykYk2dsb2JhbABBj3kBAQEBBwQGCSCcFw 17, 26 -- X-IronPort-AV: E=Sophos;i="4.25,344,1199692800"; 17, 26 -- d="scan'208";a="90460314" 17, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 17, 26 -- by ironport1.lbl.gov with ESMTP; 12 Feb 2008 21:24:40 -0800 17, 26 -- Received: from [10.0.1.6] (c-76-126-228-210.hsd1.ca.comcast.net [76.126.228.210]) 17, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id m1D5Oc5U016080; 17, 26 -- Tue, 12 Feb 2008 21:24:39 -0800 (PST) 17, 26 -- Cc: Microscopy-at-microscopy.com 17, 26 -- Message-Id: {DC3023B7-51DF-4CE1-96D3-00D4CED6C527-at-lbl.gov} 17, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 17, 26 -- To: z.zhou-at-sheffield.ac.uk 17, 26 -- In-Reply-To: {200802130001.m1D01VlZ020494-at-ns.microscopy.com} 17, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 17, 26 -- Content-Transfer-Encoding: 7bit 17, 26 -- Mime-Version: 1.0 (Apple Message framework v919.2) 17, 26 -- Subject: Re: [Microscopy] viaWWW: carbon contamination while doing EELS 17, 26 -- Date: Tue, 12 Feb 2008 17:34:42 -0800 17, 26 -- References: {200802130001.m1D01VlZ020494-at-ns.microscopy.com} 17, 26 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
The reason many microscopists don't want to use perfluorinated compounds in their electron microscopes goes back to the 1970s when diffusion pump oils based on these compounds were first introduced. (See Sect.5..4.5 p. 186 of "Vacuum Methods in Electron Microscopy") When first put into use as diffusion pump fluids it was found that these compounds broke down into small molecular fragments which were easily pumped out of the vacuum system, and were therefore essentially non-contaminating. Eureka! It was thought that the problem of oil contamination from diffusion pumps was solved. HOWEVER, after some use it was found that instruments using these fluids developed high-voltage instabilities due to micro-discharges along the ceramic insulators in their electron guns. This problem was more severe in TEMS, which operate at higher accelerating voltages, than in SEMs. About that time the Santovac polyphenyl-ether fluids came along, and most microscopists adopted them for use in their diffusion pumps..
I have not heard of anyone having this problem from the use of the Brayco or Krytox vacuum greases on O-rings, even on those in specimen stage mechanisms, however, and they are superb vacuum greases. They are excellent lubricants, they don't migrate or bleed, they are stable at temperatures up to around 250 C, and have vapor pressures in the 10^-10 Torr range.
I have not had any experience with the Santovac-5GB grease, but it sounds very interesting, too. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 14 -- From bigelow-at-umich.edu Wed Feb 13 14:16:11 2008 3, 14 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1DKGB0M005139 3, 14 -- for {microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 14:16:11 -0600 3, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 3, 14 -- BY tombraider.mr.itd.umich.edu ID 47B35009.7B868.11765 ; 3, 14 -- 13 Feb 2008 15:16:09 -0500 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06240802c3d8fa326965-at-[141.212.131.221]} 3, 14 -- Date: Wed, 13 Feb 2008 15:16:08 -0500 3, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 14 -- Subject: [Microscopy] RE: perfluorinated cmpds in EMs 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Position available at Binghamton University, one of the four university centers of the State University of New York, for an experienced electron microscopist to manage a facility that serves users in biological and materials sciences. Responsibilities include instrument maintenance (Hitachi H-7000 TEM, Hitachi S-570LB SEM), training users, service work. Applicants should have broad expertise in techniques for specimen preparation. Experience with light microscopic digital imaging also desirable. Bachelor's required; Master's preferred. Salary commensurate with education and experience. More information and application online at http:// binghamton.interviewexchange.com/. Screening of applications will begin March 1 and will continue until position is filled. The State University of New York and Binghamton University are Equal Opportunity/Affirmative Action Employers.
Dr. Curt Pueschel Associate Professor Department of Biological Sciences Binghamton University Binghamton, NY 13902-6000 607-777-2602
==============================Original Headers============================== 2, 17 -- From curtp-at-binghamton.edu Wed Feb 13 16:01:00 2008 2, 17 -- Received: from mpmail.binghamton.edu (mpmail.binghamton.edu [128.226.7.19]) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1DM0vUL021591 2, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 13 Feb 2008 16:00:59 -0600 2, 17 -- Received: from [128.226.188.61] ([128.226.188.61]) 2, 17 -- by mpmail.binghamton.edu (MOS 3.8.4-GA) 2, 17 -- with ESMTP id AZV35403; 2, 17 -- Wed, 13 Feb 2008 16:54:00 -0500 (EST) 2, 17 -- Mime-Version: 1.0 (Apple Message framework v753) 2, 17 -- Content-Transfer-Encoding: 7bit 2, 17 -- Message-Id: {D8B18AA0-C454-497F-A6ED-FE27855AAEF0-at-binghamton.edu} 2, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 2, 17 -- To: Microscopy-at-Microscopy.Com 2, 17 -- From: Curt Pueschel {curtp-at-binghamton.edu} 2, 17 -- Subject: Electron Microscopy Technician position available 2, 17 -- Date: Wed, 13 Feb 2008 16:53:59 -0500 2, 17 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
Dear List members, I am trying to source a UK or European supplier of anhydrous glutaraldehyde in acetone for use in freeze substitution protocols. Does anyone know of or have a preferred supplier? Thanks in advance. Christine. --
A.Christine.Richardson Laboratory Manager University of Durham School of Biological & Biomedical Science Centre for Molecular Imaging Science site South Rd Durham England DH1 3LE Tel: 0191 3341285\3341321 Fax: 0191 3341201 E-mail: microscopy.unit-at-dur.ac.uk
==============================Original Headers============================== 3, 28 -- From a.c.richardson-at-durham.ac.uk Wed Feb 13 16:18:23 2008 3, 28 -- Received: from hermes2.dur.ac.uk (hermes2.dur.ac.uk [129.234.8.21]) 3, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1DMINXu002200 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 16:18:23 -0600 3, 28 -- Received: from smtphost4.dur.ac.uk (smtphost4.dur.ac.uk [129.234.4.18]) 3, 28 -- by hermes2.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m1DMI6M7013729 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 22:18:11 GMT 3, 28 -- Received: from weaver.dur.ac.uk (weaver.dur.ac.uk [129.234.4.167]) 3, 28 -- by smtphost4.dur.ac.uk (8.13.7/8.13.7) with ESMTP id m1DMI6Xa024452 3, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 22:18:06 GMT 3, 28 -- Received: (from httpd-at-localhost) 3, 28 -- by weaver.dur.ac.uk (8.11.7p3+Sun/8.11.1) id m1DMI5O19476 3, 28 -- for Microscopy-at-microscopy.com; Wed, 13 Feb 2008 22:18:05 GMT 3, 28 -- X-Authentication-Warning: weaver.dur.ac.uk: httpd set sender to dbl0acr-at-smtphost.dur.ac.uk using -f 3, 28 -- Received: from host81-157-251-200.range81-157.btcentralplus.com (host81-157-251-200.range81-157.btcentralplus.com [81.157.251.200]) 3, 28 -- by webmailimpa.dur.ac.uk (IMP) with HTTP 3, 28 -- for {dbl0acr-at-imaphost.dur.ac.uk} ; Wed, 13 Feb 2008 22:18:05 +0000 3, 28 -- Message-ID: {1202941085.47b36c9da3752-at-webmailimpa.dur.ac.uk} 3, 28 -- Date: Wed, 13 Feb 2008 22:18:05 +0000 3, 28 -- From: a.c.richardson-at-durham.ac.uk 3, 28 -- To: Microscopy-at-microscopy.com 3, 28 -- Subject: Freeze substitution 3, 28 -- MIME-Version: 1.0 3, 28 -- Content-Type: text/plain; charset=ISO-8859-1 3, 28 -- Content-Transfer-Encoding: 8bit 3, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.1 3, 28 -- X-Originating-IP: 81.157.251.200 3, 28 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cogswell-at-nbnet.nb.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cogswell-at-nbnet.nb.ca Name: Steven Cogswell
Organization: University of New Brunswick Microscopy and Microanalysis Facility
Title-Subject: [Filtered] Looking for JXA-733 Parts, and to give away TEM and things
Question: Hello microscopy folks;
Hopefully you're all doing well. I'm looking to scrounge a couple of things, and I have a couple of things I'd like to give away to a good home.
What I'm really after is the "Magnification" front panel module that goes into a Jeol JXA-733 Superprobe (and I understand it's also on the 35's?). Slim box that slides into a rack, has the 10^4,10^3 etc buttons, probe scan, fine shift trims. Mine has had an unfortunate accident showing it's age and the buttons are broken. This machine is a real workhorse for us and I really wish to keep it going. If you have one of these I'd be interested in acquiring it from you. In fact, if you had other 733 bits and pieces I might be interested in those too.
As well, on a completely different instrument I'm looking for a replacement for the right-hand side persistent phosphor tv monitor that goes into a JEOL JSM-6400 (and probably a dozen other JEOL/non-JEOL units too). Mine works, but age is setting in and it's got a lot of burn-in. Does anyone have a not-so-burned in one they'd like to part with? Alternately, any cheap sources of these things?
I'm also looking for, of all things, a bell jar that goes onto an Edwards 306 carbon coater. Heck, if you had an old carbon rod holder for one (6mm/3mm, fine either way) I'd be interested in that too.
What I've got to give: In storage, I have a Philips EM201 TEM (100 kv, tungsten filament). It was working fine before it was decommissioned to be put into storage (Dec 2006). It's small by some standards (size of a largish desk, maybe 1500 lbs). It had been cleaned and reconditioned last around 2003 before being put away end of 2006.
I've got four old LKB Microtomes (Ultratomes, and an LKB Huxley). These are in various states of work/not work, and I'd rather give them away and forget about them than try and figure all that out. Lots of bits and pieces.
I've got an incomplete EMSCOPE 2000 cryo-sem unit (no sputter-coater power source, no rotary pump), with fittings to go onto a Jeol 6400, and I think fittings that went onto a microscope we never owned.
Everything I've got to give away is Free, the only caveat being you'd figure out and arrange the shipping. The TEM will fit into the back of a hefty pickup truck if you're enterprising and would like a road trip. We're in Fredericton NB, Canada. The microtomes are, as you'd expect, heavy things as well. I you're interested I can get you some more details and pictures if you'd like to contact me off list.
Best regards,
Steven Cogswell UNB Microscopy and Microanalysis Fredericton NB, Canada
Hi Christine, I can't ID the supplier you are looking for. But-- your stress on anhydrous reminds me -- I heard Paul Walther give a talk at the MSA meeting last August saying that it may not matter, it may actually preserve membranes better to have 1-5% water in the acetone, and 5% may be better than 1%.
Here is the Walther & Ziegler 2002 reference with abstract:
(2002) P. Walther and A. Ziegler Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208:3-10. Biological membranes are often poorly visible with the electron microscope after high-pressure freezing and freeze-substitution. The water content of the sample and of the substitution medium is one factor among others that strongly influences membrane visibility. In order to investigate this effect, high-pressure frozen yeast cells, rat-pancreas tissue and arthropod tissue were freeze-substituted with and without adding water to the substitution medium. The visibility of the biological membranes was generally improved if the substitution medium contained 1-5% water. The effect was especially pronounced in yeast cells, where membrane visibility was poor after freeze-substitution with water-free medium but good after addition of 5% water to the substitution medium.
Web of Science lists 30 papers citing this. I cherry-picked 12 titles, I'll supply references with with abstracts as and attachment off-list. One is a promising-sounding chapter by Kent McDonald in Vol 79 of Methods in Cell Biology (2007).
-mike reedy-
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
We have a JEM-4010, in operational condition, available for immediate aqusition. TEM only, no peripherals, film camera only. Fully serviced by JEOL engineers throughout its life. All relocation costs at your expense.
John V Nailon Operations Manager Centre for Microscopy and Microanalysis University of Queensland St.Lucia QLD 4072 Tel: 61 7 3346 3988
==============================Original Headers============================== 3, 31 -- From j.nailon-at-uq.edu.au Wed Feb 13 22:42:58 2008 3, 31 -- Received: from mailhub3.uq.edu.au (mailhub3.uq.edu.au [130.102.148.131]) 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1E4gu3q018570 3, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 22:42:57 -0600 3, 31 -- Received: from smtp3.uq.edu.au (smtp3.uq.edu.au [130.102.128.18]) 3, 31 -- by mailhub3.uq.edu.au (8.13.8/8.13.8) with ESMTP id m1E4gu9N008950 3, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 14:42:56 +1000 3, 31 -- Received: from uqexav02.soe.uq.edu.au (uqexav02.soe.uq.edu.au [130.102.4.249]) 3, 31 -- by smtp3.uq.edu.au (8.13.8/8.13.8) with ESMTP id m1E4gtE4025864 3, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 14:42:56 +1000 3, 31 -- Received: from UQEXMB1.soe.uq.edu.au ([130.102.4.216]) by uqexav02.soe.uq.edu.au with Microsoft SMTPSVC(6.0.3790.1830); 3, 31 -- Thu, 14 Feb 2008 14:42:55 +1000 3, 31 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 3, 31 -- Content-class: urn:content-classes:message 3, 31 -- MIME-Version: 1.0 3, 31 -- Content-Type: text/plain; 3, 31 -- charset="US-ASCII" 3, 31 -- Subject: JEM-4010 Available 3, 31 -- Date: Thu, 14 Feb 2008 14:42:54 +1000 3, 31 -- Message-ID: {F9BEBB3A5E6DEB498227A0739D547ADE01316DAF-at-UQEXMB1.soe.uq.edu.au} 3, 31 -- X-MS-Has-Attach: 3, 31 -- X-MS-TNEF-Correlator: 3, 31 -- Thread-topic: JEM-4010 Available 3, 31 -- Thread-index: AchuxBAbPUW2GpZQQ9Cr46m2TSz0ew== 3, 31 -- From: "John Nailon" {j.nailon-at-uq.edu.au} 3, 31 -- To: {Microscopy-at-microscopy.com} 3, 31 -- X-OriginalArrivalTime: 14 Feb 2008 04:42:55.0846 (UTC) FILETIME=[11084860:01C86EC4] 3, 31 -- X-UQ-FilterTime: 1202964176 3, 31 -- X-Scanned-By: MIMEDefang 2.58 on UQ Mailhub on 130.102.148.131 3, 31 -- Content-Transfer-Encoding: 8bit 3, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1E4gu3q018570 ==============================End of - Headers==============================
I have searched the archives and have found topics/answers that electron microscopy is not a threat for the unborn given the EM is monitored to have no leak.
However, browsing the web I could not find any information whether cathodoluminescence is as safe as well? We have a CL for installation very soon and I am hoping I could get an insight on this matter.
Thanks, Melina
==============================Original Headers============================== 6, 27 -- From mmiralles-at-pi.ac.ae Wed Feb 13 23:45:11 2008 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1E5j97w032331 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 23:45:10 -0600 6, 27 -- X-IronPort-AV: E=Sophos;i="4.25,350,1199649600"; 6, 27 -- d="scan'208";a="10672" 6, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 6, 27 -- by mx1.pi.ac.ae with ESMTP; 14 Feb 2008 09:39:29 +0400 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Thu, 14 Feb 2008 09:45:08 +0400 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="us-ascii" 6, 27 -- Subject: Off-topic: Cathodoluminescence Microscopy & Pregnancy 6, 27 -- Date: Thu, 14 Feb 2008 09:45:07 +0400 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B03D7FCA2-at-pi-exm.PI.AC.AE} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Off-topic: Cathodoluminescence Microscopy & Pregnancy 6, 27 -- Thread-Index: AchuzMFtuhAcRzwJQGS5WCawNyDeiQ== 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 14 Feb 2008 05:45:08.0356 (UTC) FILETIME=[C1C81C40:01C86ECC] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1E5j97w032331 ==============================End of - Headers==============================
} I am trying to source a UK or European supplier of anhydrous glutaraldehyde in acetone for use in freeze substitution protocols. Does anyone know of or have a preferred supplier? Thanks in advance.
The only source I know is Electron Microscopy Sciences www.emsdiasum.com
I have no interest in this company - just satisfied customer.
BTW: the necessity to use of anhydrous media for freeze-substitution is at least 'not clear'. Walther and Ziegler described in 2002 in J.Microscopy 208: 3-10 that freeze-substitution media may contain some 1 to 5 % water, and found the membranes to be visualized very nicely - better than without water. We have results supporting this finding.
best regards,
Reinhard Rachel
--
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 10, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Feb 14 01:26:47 2008 10, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1E7QltZ014820 10, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Feb 2008 01:26:47 -0600 10, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 10, 25 -- by localhost (Postfix) with SMTP id 74F94584C7 10, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Feb 2008 08:26:48 +0100 (CET) 10, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 10, 25 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id 6DE4E5559E 10, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Feb 2008 08:26:48 +0100 (CET) 10, 25 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 10, 25 -- with Novell_GroupWise; Thu, 14 Feb 2008 08:26:46 +0100 10, 25 -- Message-Id: {47B3FB3E.044C.0054.0-at-biologie.uni-regensburg.de} 10, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 10, 25 -- Date: Thu, 14 Feb 2008 08:26:38 +0100 10, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 10, 25 -- To: {Microscopy-at-Microscopy.Com} 10, 25 -- Subject: [Microscopy] Freeze substitution 10, 25 -- References: {200802132218.m1DMIeau002624-at-ns.microscopy.com} 10, 25 -- In-Reply-To: {200802132218.m1DMIeau002624-at-ns.microscopy.com} 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset=US-ASCII 10, 25 -- Content-Disposition: inline 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1E7QltZ014820 ==============================End of - Headers==============================
I concur to this, though indirectly. I also heard the talk of Prof. Walther (in Germany) and when I talked about it to a friend, he said he already tried this solution and also found some advantage to add a few water.
Regards,
Stephane
----- Original Message ---- X-from: "mike.reedy-at-cellbio.duke.edu" {mike.reedy-at-cellbio.duke.edu} To: nizets2-at-yahoo.com Sent: Thursday, February 14, 2008 4:00:25 AM
Hi Christine, I can't ID the supplier you are looking for. But-- your stress on anhydrous reminds me -- I heard Paul Walther give a talk at the MSA meeting last August saying that it may not matter, it may actually preserve membranes better to have 1-5% water in the acetone, and 5% may be better than 1%.
Here is the Walther & Ziegler 2002 reference with abstract:
(2002) P. Walther and A. Ziegler Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208:3-10. Biological membranes are often poorly visible with the electron microscope after high-pressure freezing and freeze-substitution. The water content of the sample and of the substitution medium is one factor among others that strongly influences membrane visibility. In order to investigate this effect, high-pressure frozen yeast cells, rat-pancreas tissue and arthropod tissue were freeze-substituted with and without adding water to the substitution medium. The visibility of the biological membranes was generally improved if the substitution medium contained 1-5% water. The effect was especially pronounced in yeast cells, where membrane visibility was poor after freeze-substitution with water-free medium but good after addition of 5% water to the substitution medium.
Web of Science lists 30 papers citing this. I cherry-picked 12 titles, I'll supply references with with abstracts as and attachment off-list. One is a promising-sounding chapter by Kent McDonald in Vol 79 of Methods in Cell Biology (2007).
-mike reedy-
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
==============================Original Headers============================== 11, 22 -- From mike.reedy-at-cellbio.duke.edu Wed Feb 13 20:54:57 2008 11, 22 -- Received: from athos.duhs.duke.edu (athos.duhs.duke.edu [152.16.195.41]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1E2svOj003255 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 20:54:57 -0600 11, 22 -- Received: from cellbio.duke.edu (voice.cellbio.duke.edu [152.16.14.127]) 11, 22 -- by athos.duhs.duke.edu (8.13.4/8.13.4) with ESMTP id m1E2sfGH7639208 11, 22 -- (version=TLSv1/SSLv3 cipher=DES-CBC3-SHA bits=168 verify=NO); 11, 22 -- Wed, 13 Feb 2008 21:54:41 -0500 11, 22 -- Received: from [152.16.179.69] (HELO [172.17.186.142]) 11, 22 -- by cellbio.duke.edu (CommuniGate Pro SMTP 4.1.8) 11, 22 -- with ESMTP id 5353057; Wed, 13 Feb 2008 21:54:40 -0500 11, 22 -- Mime-Version: 1.0 11, 22 -- Message-Id: {p06230955c3d953b60e7e-at-[172.17.186.142]} 11, 22 -- In-Reply-To: {200802132221.m1DMLLfR007452-at-ns.microscopy.com} 11, 22 -- References: {200802132221.m1DMLLfR007452-at-ns.microscopy.com} 11, 22 -- Date: Wed, 13 Feb 2008 21:54:23 -0500 11, 22 -- To: a.c.richardson-at-durham.ac.uk 11, 22 -- From: Mike Reedy {mike.reedy-at-cellbio.duke.edu} 11, 22 -- Subject: Re: [Microscopy] Freeze substitution 11, 22 -- Cc: "Microscopy Listserver" {microscopy-at-microscopy.com} 11, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 22 -- X-Scanned-By: MIMEDefang 2.51 on 152.16.195.41 ==============================End of - Headers==============================
____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
==============================Original Headers============================== 23, 19 -- From nizets2-at-yahoo.com Thu Feb 14 01:45:49 2008 23, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 23, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1E7jnNX028018 23, 19 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 01:45:49 -0600 23, 19 -- Received: (qmail 5164 invoked by uid 60001); 14 Feb 2008 07:45:49 -0000 23, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 19 -- s=s1024; d=yahoo.com; 23, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 23, 19 -- b=xVqNq46d1B2vS6AXMBYheLIANRL25CfYJFm+wR15JENgKdpmmyZl41wQqlVMwP6V3sMMrb/h675tb4MPHgB0/HwizkmdQ1+5kNegveNe7lBTBLyTLWhkFDSufDE02sxy+P+fAGyzIDCklGBMBkkQoTWx6VhN869vnaBBL4X3h0s=; 23, 19 -- X-YMail-OSG: OYdeIAgVM1mh6j0aLJABlLg9pVjjMosqxqi6CahJGwTNagGBRmzjdenkGiSjEbjSN6Z_p2cAGlxe5Qx81d4B4bHUoAa7aZXxVKZuYpj64d_PCB3J8gtb_qPdLbK2878YmBZha6iFQ.fTwNHHR4fw2jKHhofrEwDaZdyQLdoirU05 23, 19 -- Received: from [80.122.101.100] by web37405.mail.mud.yahoo.com via HTTP; Wed, 13 Feb 2008 23:45:48 PST 23, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 23, 19 -- Date: Wed, 13 Feb 2008 23:45:48 -0800 (PST) 23, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 23, 19 -- Subject: Re: [Microscopy] Re: Freeze substitution 23, 19 -- To: microscopy-at-microscopy.com 23, 19 -- MIME-Version: 1.0 23, 19 -- Content-Type: text/plain; charset=us-ascii 23, 19 -- Message-ID: {18894.855.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
We were talking about X-rays. I really doubt that photons can pass through your clothes and skin. The sunlight is probably much more intense that the feable light coming from the fluorescent screen. Or perhaps I did not understand the message?
Best regards, Stephane
----- Original Message ---- X-from: "mmiralles-at-pi.ac.ae" {mmiralles-at-pi.ac.ae} To: nizets2-at-yahoo.com Sent: Thursday, February 14, 2008 6:50:35 AM
Hello,
I have searched the archives and have found topics/answers that electron microscopy is not a threat for the unborn given the EM is monitored to have no leak.
However, browsing the web I could not find any information whether cathodoluminescence is as safe as well? We have a CL for installation very soon and I am hoping I could get an insight on this matter.
Thanks, Melina
==============================Original Headers============================== 6, 27 -- From mmiralles-at-pi.ac.ae Wed Feb 13 23:45:11 2008 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1E5j97w032331 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 23:45:10 -0600 6, 27 -- X-IronPort-AV: E=Sophos;i="4.25,350,1199649600"; 6, 27 -- d="scan'208";a="10672" 6, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 6, 27 -- by mx1.pi.ac.ae with ESMTP; 14 Feb 2008 09:39:29 +0400 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Thu, 14 Feb 2008 09:45:08 +0400 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="us-ascii" 6, 27 -- Subject: Off-topic: Cathodoluminescence Microscopy & Pregnancy 6, 27 -- Date: Thu, 14 Feb 2008 09:45:07 +0400 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B03D7FCA2-at-pi-exm.PI.AC.AE} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Off-topic: Cathodoluminescence Microscopy & Pregnancy 6, 27 -- Thread-Index: AchuzMFtuhAcRzwJQGS5WCawNyDeiQ== 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 14 Feb 2008 05:45:08.0356 (UTC) FILETIME=[C1C81C40:01C86ECC] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1E5j97w032331 ==============================End of - Headers==============================
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==============================Original Headers============================== 16, 20 -- From nizets2-at-yahoo.com Thu Feb 14 01:52:02 2008 16, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1E7q055004369 16, 20 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 01:52:02 -0600 16, 20 -- Received: (qmail 41355 invoked by uid 60001); 14 Feb 2008 07:52:00 -0000 16, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 20 -- s=s1024; d=yahoo.com; 16, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 16, 20 -- b=FLN70u3uATslt54BH0ILwXLaRBDR05qxi2ZS48agsjkapTHqZvl1+TNbTrqUGq30g6fcW0SLAjR+kW7aPpUDk9VvXPRq0OTfkE6SAFlFPwyw9RM33v9WiE0vYhMK75SvPfKoLO7GzSIXmzPUKkSi01WRQFR9/ZT4s3rdnaz6MCU=; 16, 20 -- X-YMail-OSG: L2X9jlgVM1n_UvfjM.Sgg4TgKUIv10yDgq88YPZYx7Dj3ze3FHsBKH7qrrc._MoLfJDy4TyifId7RMcoLmbkvsgc5Vtydefx_.Oz.KylGJfdgUogaMZilkOKkqNcGPSq6aXpMhNfJmelzkirNT4eJfEsjmfUctGNSIzByVc7x4tb 16, 20 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Wed, 13 Feb 2008 23:52:00 PST 16, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 16, 20 -- Date: Wed, 13 Feb 2008 23:52:00 -0800 (PST) 16, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 16, 20 -- Subject: Re: [Microscopy] Off-topic: Cathodoluminescence Microscopy & Pregnancy 16, 20 -- To: mmiralles-at-pi.ac.ae 16, 20 -- Cc: microscopy-at-microscopy.com 16, 20 -- MIME-Version: 1.0 16, 20 -- Content-Type: text/plain; charset=us-ascii 16, 20 -- Message-ID: {394157.39063.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
Dear List members, You are all of course right about the addition of small amounts of water. We get great results with up to 5% water. I apologies if my question was badly worded. I am really looking for a local supplier of 50 % or 70% glutaraldeyde in acetone. ( Can only think it was late when I wrote the email and had been reading papers detailing the use of anhydrous glutaraldeyde). Thank you for so many responses, as usual this list comes up trumps. Regards Christine.
School of Biological & Biomedical Science Centre for Molecular Imaging University of Durham Science site South Rd Durham England DH1 3LE Tel: 0191 3341285\3341321 Fax: 0191 3341201 E-mail: microscopy.unit-at-dur.ac.uk
==============================Original Headers============================== 4, 31 -- From a.c.richardson-at-durham.ac.uk Thu Feb 14 05:17:03 2008 4, 31 -- Received: from hermes1.dur.ac.uk (hermes1.dur.ac.uk [129.234.8.20]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EBH2GI029341 4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 05:17:03 -0600 4, 31 -- Received: from smtphost3.dur.ac.uk (smtphost3.dur.ac.uk [129.234.4.55]) 4, 31 -- by hermes1.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m1EBGkXM027519 4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 11:16:50 GMT 4, 31 -- Received: from weevil.dur.ac.uk (weevil.dur.ac.uk [129.234.8.4]) 4, 31 -- by smtphost3.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m1EBGgqd026134 4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 11:16:42 GMT 4, 31 -- Received: from weevil.dur.ac.uk (localhost [127.0.0.1]) 4, 31 -- by weevil.dur.ac.uk (8.13.6+Sun/8.11.1) with ESMTP id m1EBGgnT021811 4, 31 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 11:16:42 GMT 4, 31 -- Received: (from httpd-at-localhost) 4, 31 -- by weevil.dur.ac.uk (8.13.6+Sun/8.13.6/Submit) id m1EBGgLO021808 4, 31 -- for microscopy-at-microscopy.com; Thu, 14 Feb 2008 11:16:42 GMT 4, 31 -- X-Authentication-Warning: weevil.dur.ac.uk: httpd set sender to dbl0acr-at-smtphost.dur.ac.uk using -f 4, 31 -- Received: from biosci-71.dur.ac.uk (biosci-71.dur.ac.uk [129.234.133.71]) 4, 31 -- by webmailimpc.dur.ac.uk (IMP) with HTTP 4, 31 -- for {dbl0acr-at-imaphost.dur.ac.uk} ; Thu, 14 Feb 2008 11:16:42 +0000 4, 31 -- Message-ID: {1202987802.47b4231a27017-at-webmailimpc.dur.ac.uk} 4, 31 -- Date: Thu, 14 Feb 2008 11:16:42 +0000 4, 31 -- From: a.c.richardson-at-durham.ac.uk 4, 31 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 4, 31 -- Subject: FreezeSubstitution 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; charset=ISO-8859-1 4, 31 -- Content-Transfer-Encoding: 8bit 4, 31 -- User-Agent: Internet Messaging Program (IMP) 3.2.1 4, 31 -- X-Originating-IP: 129.234.133.71 4, 31 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
I don't know if this will be useful, but the following procedure is given to make anhydrous glutaraldehyde. The methanol and acetone should not be an issue; I have seen (and can't find the reference...) that methanol can "hold" more water at typical substitution temperatures (we have used 193K).
Dale Callaham
} Anhydrous glutaraldehyde } Procedures in Electron Microscopy, Principal Editors A.W. Robards and A.J. Wilson (Wiley) } 16:6.5 Freeze Substitution with dimethoxypropane (DMP) } 1) Acidify DMP by adding 3 drops 0.1M HCl per 25 ml DMP. } 2) Shake 25% aqueous glutaraldehyde with acidified DMP (converts the water to methanol and acetone) until the solution is clear. } 3) Add acetone (or methanol) and uranyl acetate, and cool to 200K. } 4) Dissolve the Osmium crystals in the medium at 200K.
reinhard.rachel-at-biologie.uni-regensburg.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I am trying to source a UK or European supplier of anhydrous glutaraldehyde in } acetone for use in freeze substitution protocols. } Does anyone know of or have a preferred supplier? } Thanks in advance. } } The only source I know is Electron Microscopy Sciences } www.emsdiasum.com } } I have no interest in this company - just satisfied customer. } } BTW: the necessity to use of anhydrous media for freeze-substitution is at least 'not clear'. } Walther and Ziegler described in 2002 in J.Microscopy 208: 3-10 that freeze-substitution media may contain some 1 to 5 % water, and found the membranes to be visualized very nicely - better than without water. } We have results supporting this finding. } } best regards, } } Reinhard Rachel }
==============================Original Headers============================== 7, 22 -- From dac-at-research.umass.edu Thu Feb 14 08:39:31 2008 7, 22 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EEdVDv016217 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 08:39:31 -0600 7, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 22 -- (authenticated bits=0) 7, 22 -- by race4.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1EEdUA6019104 7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 09:39:30 -0500 7, 22 -- Message-ID: {47B4536E.6070304-at-research.umass.edu} 7, 22 -- Date: Thu, 14 Feb 2008 09:42:54 -0500 7, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 22 -- Reply-To: dac-at-research.umass.edu 7, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 7, 22 -- MIME-Version: 1.0 7, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 22 -- Subject: Re: [Microscopy] Freeze substitution 7, 22 -- References: {200802140733.m1E7Xoom023849-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {200802140733.m1E7Xoom023849-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
I am exploring whether I can digitally capture video from an old high- resolution CRT. The CRT in question is connected to an electron microscope (JEOL JXA-8900R) and is used for taking Polaroid photos of the electron image. The CRT has a resolution of 1940 lines, but the scan rate is very slow: one frame in about 70 seconds. The rear of the CRT has two BNC connectors that apparently connected to the main viewing CRT on the microscope, and the only other connection seems to be the high voltage. I have found two commercial "frame grabbers" that cost $8000+ which could do capture this image signal and feed it into a computer, but I'd like to consider possibly cheaper or better alternatives. Does anyone have any experience with or ideas about digitally capturing this video signal and feeding it into a computer for viewing, storing, etc? I welcome any advice you might have.
Thanks, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu
==============================Original Headers============================== 4, 17 -- From frah0010-at-umn.edu Thu Feb 14 09:49:02 2008 4, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EFn2sC031041 4, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 09:49:02 -0600 4, 17 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu [160.94.146.133]) 4, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 4, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 09:48:56 -0600 (CST) 4, 17 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU 4, 17 -- Message-Id: {CE38E0D1-54A2-4595-8B13-2C010139FA6E-at-umn.edu} 4, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} 4, 17 -- To: microscopy-at-microscopy.com 4, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 17 -- Content-Transfer-Encoding: 7bit 4, 17 -- Mime-Version: 1.0 (Apple Message framework v919.2) 4, 17 -- Subject: Digital capture of CRT 4, 17 -- Date: Thu, 14 Feb 2008 09:48:55 -0600 4, 17 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
This is generally called "passive capture" - letting the microscope drive the scan and you just tap off the signals. I built a system for our JEOL JSM-5400 that we used for several years. It had 1920x1440 resolution - sample image: (http://www.bio.umass.edu/microscopy/images/pcb.tif).
You are welcome to any of the information/code/schematics.
Basically it detected the extended screen blanking signal that occurs on the JSM-5400 when Shutter is pressed to start an image. It then used the horizontal unblanking to gate a clock on the DMA board as a pixel clock to initiate the sampling (A/D) and each conversion completion triggered a DMA transfer to the PC DMA buffer. The horizontal blanking (end of line) signal triggered moving the "line" of data from the real memory DMA buffer (remember DOS and 64k?) to XMS. When the lines were all done, it prompted to save as TIF format.
I got away with an inexpensive 8-bit A/D converter (Maxim MAX-165) since the SEM controls for optimum brightness and contrast were matched to the full-scale input of the A/D converter.
There were some prescan/postscan lines and delays I worked out so it captured accurately what was centered on the CRT with the proper aspect ratio.
I have dropped this project beacuse it was written in assembly language, required DOS mode and a $250 DMA card (the capture circuit costs ~$30) and so this was getting difficult to support. People seemed interested but needed help building and setting up and I didn't have time for that. I was thinking of using an EPP (parallel) connection to avoid DMA but that was also dealing with "ports" in a way modern operating systems like to block.
I have been thinking about porting the process to a USB connection that will avoid the DMA card and would be more supportable. FTDI makes a chip that provides a direct USB interface and a FIFO interface for a converter/microcontroller side. They provide free a driver so that Visual Basic, etc. can interface with the operating system in a civil and acceptable way to collect the data and save to disk. Sparkfun Electronics has a "breakout board" for this chip ($18) that should ease development: http://www.sparkfun.com/commerce/product_info.php?products_id=7841 and they list the drivers and datasheets.
I have no economic interest in any of the products mentioned by name.
Cheers!
Dale Callaham
frah0010-at-umn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } I am exploring whether I can digitally capture video from an old high- } resolution CRT. The CRT in question is connected to an electron } microscope (JEOL JXA-8900R) and is used for taking Polaroid photos of } the electron image. The CRT has a resolution of 1940 lines, but the } scan rate is very slow: one frame in about 70 seconds. The rear of } the CRT has two BNC connectors that apparently connected to the main } viewing CRT on the microscope, and the only other connection seems to } be the high voltage. I have found two commercial "frame grabbers" } that cost $8000+ which could do capture this image signal and feed it } into a computer, but I'd like to consider possibly cheaper or better } alternatives. Does anyone have any experience with or ideas about } digitally capturing this video signal and feeding it into a computer } for viewing, storing, etc? I welcome any advice you might have. } } Thanks, } Ellery } } -------------------- } Ellery E. Frahm } Research Fellow & Manager } Electron Microprobe Laboratory } University of Minnesota - Twin Cities } Department of Geology & Geophysics } Lab Website: http://probelab.geo.umn.edu } } ==============================Original Headers============================== } 4, 17 -- From frah0010-at-umn.edu Thu Feb 14 09:49:02 2008 } 4, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) } 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EFn2sC031041 } 4, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 09:49:02 -0600 } 4, 17 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu [160.94.146.133]) } 4, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP } 4, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 09:48:56 -0600 (CST) } 4, 17 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU } 4, 17 -- Message-Id: {CE38E0D1-54A2-4595-8B13-2C010139FA6E-at-umn.edu} } 4, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} } 4, 17 -- To: microscopy-at-microscopy.com } 4, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 4, 17 -- Content-Transfer-Encoding: 7bit } 4, 17 -- Mime-Version: 1.0 (Apple Message framework v919.2) } 4, 17 -- Subject: Digital capture of CRT } 4, 17 -- Date: Thu, 14 Feb 2008 09:48:55 -0600 } 4, 17 -- X-Mailer: Apple Mail (2.919.2) } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 22 -- From dac-at-research.umass.edu Thu Feb 14 10:44:56 2008 14, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EGiuLK013632 14, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 10:44:56 -0600 14, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 14, 22 -- (authenticated bits=0) 14, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1EGitnS017505 14, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 14, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 11:44:55 -0500 14, 22 -- Message-ID: {47B470D3.8040900-at-research.umass.edu} 14, 22 -- Date: Thu, 14 Feb 2008 11:48:19 -0500 14, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 14, 22 -- Reply-To: dac-at-research.umass.edu 14, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 14, 22 -- MIME-Version: 1.0 14, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 14, 22 -- Subject: Re: [Microscopy] Digital capture of CRT 14, 22 -- References: {200802141555.m1EFtar1007700-at-ns.microscopy.com} 14, 22 -- In-Reply-To: {200802141555.m1EFtar1007700-at-ns.microscopy.com} 14, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 22 -- Content-Transfer-Encoding: 7bit 14, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
While there does seem to be some evidence suggesting a bit of water in the freeze sub mixture creates better contrast we have found that this generally results in 'allowing' some ice nucleation which creates the better contrast by separating the membranes from the cytoplasmic components but also increases your chances of getting unsuitable artifacts. We started adding 0.1% tannic acid to the initial acetone freeze sub mixture and after a couple of days of substitution we wash out the free tannic acid with clean acetone before adding any osmium. This resulted in nice membranes. Sorry but I do not have any published pictures using this, as of yet, just my two cents worth.
Garnet
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Garnet Martens
Research Manager BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-3354
==============================Original Headers============================== 8, 24 -- From gmartens-at-interchange.ubc.ca Thu Feb 14 11:00:35 2008 8, 24 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EH0YDG026453 8, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 11:00:35 -0600 8, 24 -- X-Ubc-Received: from mr5.mail-relay.ubc.ca (localhost [127.0.0.1]) 8, 24 -- by localhost (Postfix) with SMTP id 6564D11468 8, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 09:00:34 -0800 (PST) 8, 24 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 8, 24 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP 8, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 09:00:31 -0800 (PST) 8, 24 -- Date: Thu, 14 Feb 2008 09:00:29 -0800 8, 24 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} 8, 24 -- Subject: [Microscopy] Re: Freeze substitution 8, 24 -- In-reply-to: {200802141443.m1EEhhC2021678-at-ns.microscopy.com} 8, 24 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 8, 24 -- Message-id: {a06240802c3da22c766cf-at-[128.189.217.253]} 8, 24 -- MIME-version: 1.0 8, 24 -- Content-type: text/plain; format=flowed; charset=us-ascii 8, 24 -- References: {200802141443.m1EEhhC2021678-at-ns.microscopy.com} 8, 24 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.2.14.84746 8, 24 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 8, 24 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0 8, 24 -- X-Spam-Level: 8, 24 -- X-Spam-Flag: No ==============================End of - Headers==============================
I will contact clients who we did the work for to find out if they would like to write it up, or if they will let us write it up.
We wash out the tannic acid to avoid any ppt with the osmium. Quite often we would get a ppt, even at -20 - -30 which caused rather severe headaches when trying to recover the little bits of tissue or cells after HPF. We found that by washing a couple of times with clean acetone left us with a nice clean prep. The ppt does not affect the ultrastructure, it's just messy.
Cheers,
} Garnet, } } Were you planning on publishing this in a } peer-reviewed primary research journal? If that } is not a concern, this would make a good article } for Microscopy Today. Would you be interested in } writing it up for us? Being optimistic, I have } attached a copy of our Instructions to Authors. } } I first ran into this issue of leaving water in } the freeze-substitution fluids back in '03 or } so, and wondered why it worked. I have a } question though: why wash out the tannic acid? I } routinely use 1% tannic acid in OsO4 for } membrane preservation during chemical fixation } (no freeze-subsitution), why not leave it in } during freeze-substituition? } } Thank you for your attention. } } Phil } } } While there does seem to be some evidence suggesting a bit of water } } in the freeze sub mixture creates better contrast we have found that } } this generally results in 'allowing' some ice nucleation which } } creates the better contrast by separating the membranes from the } } cytoplasmic components but also increases your chances of getting } } unsuitable artifacts. We started adding 0.1% tannic acid to the } } initial acetone freeze sub mixture and after a couple of days of } } substitution we wash out the free tannic acid with clean acetone } } before adding any osmium. This resulted in nice membranes. Sorry } } but I do not have any published pictures using this, as of yet, just } } my two cents worth. } } } } Garnet } } -- } } Garnet Martens } } } } Research Manager } } BioImaging Facility } } University of British Columbia } } 6270 University Blvd. } } Vancouver, B.C. } } Canada } } V6T 1Z4 } } } } phone 604-822-3354 } -- } Philip Oshel } Technical Editor, Microscopy Today } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } } Attachment converted: Garnet's computer: } MT_Instructions to authors.pdf (PDF /«IC») } (001087A5)
-- Garnet Martens
Research Manager BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-3354
==============================Original Headers============================== 11, 28 -- From gmartens-at-interchange.ubc.ca Thu Feb 14 11:40:10 2008 11, 28 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca [137.82.45.15]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EHe9sw007712 11, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 11:40:10 -0600 11, 28 -- X-Ubc-Received: from mr8.mail-relay.ubc.ca (localhost [127.0.0.1]) 11, 28 -- by localhost (Postfix) with SMTP id 3710910BFD 11, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 09:40:09 -0800 (PST) 11, 28 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 11, 28 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP 11, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 09:40:03 -0800 (PST) 11, 28 -- Date: Thu, 14 Feb 2008 09:37:20 -0800 11, 28 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} 11, 28 -- Subject: [Microscopy] Re: Freeze substitution 11, 28 -- In-reply-to: {f06240807c3da271bc7f2-at-[141.209.160.249]} 11, 28 -- To: Philip Oshel {oshel1pe-at-cmich.edu} 11, 28 -- Cc: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 11, 28 -- Message-id: {a06240803c3da2c0891d0-at-[128.189.217.253]} 11, 28 -- MIME-version: 1.0 11, 28 -- Content-type: text/plain; format=flowed; charset=iso-8859-1 11, 28 -- References: {200802141704.m1EH4i9W000417-at-ns.microscopy.com} 11, 28 -- {f06240807c3da271bc7f2-at-[141.209.160.249]} 11, 28 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.2.14.92441 11, 28 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 11, 28 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_PHRASE5 0, __SANE_MSGID 0 11, 28 -- X-Spam-Level: 11, 28 -- X-Spam-Flag: No 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1EHe9sw007712 ==============================End of - Headers==============================
Check out references for water in the prep (works very well in our hands) from Walther et al. One is:
Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. Journal of Microscopy, Vol. 208, Pt 1 October 2002, pp. 310
One excellent review of methodology is by Tom Giddings at U. of Colorado. This is most certainly not the first reference for addition of tannic acid but should help you find original references.
Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. Journal of Microscopy, Vol. 212, Pt 1 October 2003, pp. 5361
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
} From: {gmartens-at-interchange.ubc.ca} } Reply-To: {gmartens-at-interchange.ubc.ca} } Date: Thu, 14 Feb 2008 11:42:32 -0600 } To: {dsherman-at-purdue.edu} } Subject: [Microscopy] Re: Freeze substitution } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Phil, } } I will contact clients who we did the work for to } find out if they would like to write it up, or if } they will let us write it up. } } We wash out the tannic acid to avoid any ppt with } the osmium. Quite often we would get a ppt, even } at -20 - -30 which caused rather severe headaches } when trying to recover the little bits of tissue } or cells after HPF. We found that by washing a } couple of times with clean acetone left us with a } nice clean prep. The ppt does not affect the } ultrastructure, it's just messy. } } Cheers, } } } } Garnet, } } } } Were you planning on publishing this in a } } peer-reviewed primary research journal? If that } } is not a concern, this would make a good article } } for Microscopy Today. Would you be interested in } } writing it up for us? Being optimistic, I have } } attached a copy of our Instructions to Authors. } } } } I first ran into this issue of leaving water in } } the freeze-substitution fluids back in '03 or } } so, and wondered why it worked. I have a } } question though: why wash out the tannic acid? I } } routinely use 1% tannic acid in OsO4 for } } membrane preservation during chemical fixation } } (no freeze-subsitution), why not leave it in } } during freeze-substituition? } } } } Thank you for your attention. } } } } Phil } } } } } While there does seem to be some evidence suggesting a bit of water } } } in the freeze sub mixture creates better contrast we have found that } } } this generally results in 'allowing' some ice nucleation which } } } creates the better contrast by separating the membranes from the } } } cytoplasmic components but also increases your chances of getting } } } unsuitable artifacts. We started adding 0.1% tannic acid to the } } } initial acetone freeze sub mixture and after a couple of days of } } } substitution we wash out the free tannic acid with clean acetone } } } before adding any osmium. This resulted in nice membranes. Sorry } } } but I do not have any published pictures using this, as of yet, just } } } my two cents worth. } } } } } } Garnet } } } -- } } } Garnet Martens } } } } } } Research Manager } } } BioImaging Facility } } } University of British Columbia } } } 6270 University Blvd. } } } Vancouver, B.C. } } } Canada } } } V6T 1Z4 } } } } } } phone 604-822-3354 } } -- } } Philip Oshel } } Technical Editor, Microscopy Today } } Microscopy Facility Supervisor } } Biology Department } } 024C Brooks Hall } } Central Michigan University } } Mt. Pleasant, MI 48859 } } (989) 774-3576 } } } } } } Attachment converted: Garnet's computer: } } MT_Instructions to authors.pdf (PDF /«IC») } } (001087A5) } } } -- } Garnet Martens } } Research Manager } BioImaging Facility } University of British Columbia } 6270 University Blvd. } Vancouver, B.C. } Canada } V6T 1Z4 } } phone 604-822-3354 } } } ==============================Original Headers============================== } 11, 28 -- From gmartens-at-interchange.ubc.ca Thu Feb 14 11:40:10 2008 } 11, 28 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca } [137.82.45.15]) } 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m1EHe9sw007712 } 11, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 11:40:10 } -0600 } 11, 28 -- X-Ubc-Received: from mr8.mail-relay.ubc.ca (localhost [127.0.0.1]) } 11, 28 -- by localhost (Postfix) with SMTP id 3710910BFD } 11, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 09:40:09 } -0800 (PST) } 11, 28 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca } [142.103.145.70]) } 11, 28 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP } 11, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 09:40:03 } -0800 (PST) } 11, 28 -- Date: Thu, 14 Feb 2008 09:37:20 -0800 } 11, 28 -- From: Garnet Martens {gmartens-at-interchange.ubc.ca} } 11, 28 -- Subject: [Microscopy] Re: Freeze substitution } 11, 28 -- In-reply-to: {f06240807c3da271bc7f2-at-[141.209.160.249]} } 11, 28 -- To: Philip Oshel {oshel1pe-at-cmich.edu} } 11, 28 -- Cc: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} } 11, 28 -- Message-id: {a06240803c3da2c0891d0-at-[128.189.217.253]} } 11, 28 -- MIME-version: 1.0 } 11, 28 -- Content-type: text/plain; format=flowed; charset=iso-8859-1 } 11, 28 -- References: {200802141704.m1EH4i9W000417-at-ns.microscopy.com} } 11, 28 -- {f06240807c3da271bc7f2-at-[141.209.160.249]} } 11, 28 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: } 2.6.0.325393, Antispam-Data: 2008.2.14.92441 } 11, 28 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca } 11, 28 -- X-PerlMx-Spam: Probability=7%, Report=__C230066_P5 0, __CT 0, __CTE } 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, } __PHISH_PHRASE5 0, __SANE_MSGID 0 } 11, 28 -- X-Spam-Level: } 11, 28 -- X-Spam-Flag: No } 11, 28 -- Content-Transfer-Encoding: 8bit } 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m1EHe9sw007712 } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 25 -- From dsherman-at-purdue.edu Thu Feb 14 11:54:41 2008 13, 25 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EHseVQ020797 13, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 11:54:41 -0600 13, 25 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 13, 25 -- Thu, 14 Feb 2008 12:54:40 -0500 13, 25 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 13, 25 -- Thu, 14 Feb 2008 17:54:40 +0000 13, 25 -- User-Agent: Microsoft-Entourage/11.3.6.070618 13, 25 -- Date: Thu, 14 Feb 2008 12:54:38 -0500 13, 25 -- Subject: Re: [Microscopy] Re: Freeze substitution 13, 25 -- From: Debby Sherman {dsherman-at-purdue.edu} 13, 25 -- To: {gmartens-at-interchange.ubc.ca} , 13, 25 -- "message to: MSA list" {microscopy-at-microscopy.com} 13, 25 -- CC: Philip Oshel {philip.oshel-at-cmich.edu} 13, 25 -- Message-ID: {C3D9EA8E.28800%dsherman-at-purdue.edu} 13, 25 -- Thread-Topic: [Microscopy] Re: Freeze substitution 13, 25 -- Thread-Index: AchvMqqF6V3rmtslEdy8gwARJN08Mg== 13, 25 -- In-Reply-To: {200802141742.m1EHgW3N011185-at-ns.microscopy.com} 13, 25 -- Mime-version: 1.0 13, 25 -- Content-type: text/plain; 13, 25 -- charset="ISO-8859-1" 13, 25 -- X-OriginalArrivalTime: 14 Feb 2008 17:54:40.0703 (UTC) FILETIME=[AC2234F0:01C86F32] 13, 25 -- Content-Transfer-Encoding: 8bit 13, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1EHseVQ020797 ==============================End of - Headers==============================
I would agree that you probably want a passive capture system. Normal frame grabbers are passive systems, but they are normally designed for capturing NTSC TV images. For $8000, maybe that system is more flexible and could match the full resolution of your scope.
We happen to use a system from Quartz PCI to passively capture images on one of our scopes. I think the cost was a bit more than $8000. {g} However, I believe it was money well spent. The imaging tools that are built into the program and the file handling are worth the extra cost.
Disclaimer: I have no financial interest in the company. There are other systems available from other companies that are probably worth considering.
Warren Straszheim Iowa State University
-----Original Message----- X-from: frah0010-at-umn.edu [mailto:frah0010-at-umn.edu] Sent: Thursday, February 14, 2008 9:50 AM To: wesaia-at-iastate.edu
Hello,
I am exploring whether I can digitally capture video from an old high- resolution CRT. The CRT in question is connected to an electron microscope (JEOL JXA-8900R) and is used for taking Polaroid photos of the electron image. The CRT has a resolution of 1940 lines, but the scan rate is very slow: one frame in about 70 seconds. The rear of the CRT has two BNC connectors that apparently connected to the main viewing CRT on the microscope, and the only other connection seems to be the high voltage. I have found two commercial "frame grabbers" that cost $8000+ which could do capture this image signal and feed it into a computer, but I'd like to consider possibly cheaper or better alternatives. Does anyone have any experience with or ideas about digitally capturing this video signal and feeding it into a computer for viewing, storing, etc? I welcome any advice you might have.
Thanks, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu
==============================Original Headers============================== 14, 37 -- From wesaia-at-iastate.edu Thu Feb 14 12:03:19 2008 14, 37 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 14, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EI3Jcm000964 14, 37 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 14 Feb 2008 12:03:19 -0600 14, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 14, 37 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m1EI3IVi013383; 14, 37 -- Thu, 14 Feb 2008 12:03:18 -0600 14, 37 -- Received: from (despam-11.iastate.edu [129.186.140.81]) by devirus-10.iastate.edu with smtp 14, 37 -- id 6f4c_193328b4_db27_11dc_82ce_00137253420a; 14, 37 -- Thu, 14 Feb 2008 12:03:07 -0600 14, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 14, 37 -- by despam-11.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m1EI3AOs019011; 14, 37 -- Thu, 14 Feb 2008 12:03:15 -0600 14, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 37 -- Thu, 14 Feb 2008 12:03:14 -0600 14, 37 -- Content-class: urn:content-classes:message 14, 37 -- MIME-Version: 1.0 14, 37 -- Content-Type: text/plain; 14, 37 -- charset="us-ascii" 14, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 37 -- Subject: RE: [Microscopy] Digital capture of CRT 14, 37 -- Date: Thu, 14 Feb 2008 12:03:27 -0600 14, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72702884A85-at-maire.eng.iastate.edu} 14, 37 -- In-Reply-To: {200802141549.m1EFnx6p032224-at-ns.microscopy.com} 14, 37 -- X-MS-Has-Attach: 14, 37 -- X-MS-TNEF-Correlator: 14, 37 -- Thread-Topic: [Microscopy] Digital capture of CRT 14, 37 -- Thread-Index: AchvIUQIbXtslAoXTKmWIYnXD0puUQAEepmw 14, 37 -- References: {200802141549.m1EFnx6p032224-at-ns.microscopy.com} 14, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 14, 37 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 14, 37 -- Cc: {frah0010-at-umn.edu} 14, 37 -- X-OriginalArrivalTime: 14 Feb 2008 18:03:14.0320 (UTC) FILETIME=[DE45F100:01C86F33] 14, 37 -- X-PMX-Version: 5.3.1.294258, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2008.2.14.95051 14, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='__CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0' 14, 37 -- Content-Transfer-Encoding: 8bit 14, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1EI3Jcm000964 ==============================End of - Headers==============================
Is that a cathodluminescence attachment to an EM, or is that a cathodoluminescence system in association with a table-top light microscope. I remember such systems being used in mineralogy. It involved illuminating a sample with an electron beam; I presume it was in some sort of vacuum chamber, but I do not know the details. I would be concerned about shielding in such a system.
If you are adding CL to your existing microscope, you are only adding a detector. The CL is already occurring if you have the right kind of sample; you just cannot see it with your current detectors. (However, I sometimes pick up strong CL on our IR chamber scope. I cut power to the illuminator and can watch the beam scanning the sample.)
You might be concerned if the addition of a new detector allows leakage of x-rays from the detector port. It would probably be worth a check, but I doubt that you will find anything to be concerned about.
Warren
-----Original Message----- X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae] Sent: Wednesday, February 13, 2008 11:47 PM To: wesaia-at-iastate.edu
Hi, All-
I was recently asked to image some surgical burrs (tiny, diamond-chip-studded drill bits) with SEM. Two were heavily coated with tissue debris. I successfully cleaned one off with chlorine bleach, but the other corroded into an ugly mess. This type of project is outside my range of experience (I tend to avoid clinical stuff). (I know how to get tissue off coral.)
More of these burrs are coming soon. Does anyone have any suggestions for cleaning these burrs while maintaining the integrity of the steel, adhesive, and diamond chips?
Aloha from sunny, warm, etc. Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 19 -- From tina-at-pbrc.hawaii.edu Thu Feb 14 12:45:58 2008 6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EIjvUP027372 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 12:45:58 -0600 6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m1EIjr8i014728 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 08:45:53 -1000 (HST) 6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m1EIjq4c014725 6, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 14 Feb 2008 08:45:52 -1000 (HST) 6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 19 -- Date: Thu, 14 Feb 2008 08:45:51 -1000 (HST) 6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 19 -- X-Sender: tina-at-halia 6, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 19 -- Subject: Cleaning of surgical burrs for SEM 6, 19 -- Message-ID: {Pine.GSO.4.21.0802140840260.14573-100000-at-halia} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Dear Tina, The first and best cleaning would be to ultrasonically clean the drill bit in alcohol for a minute or two. This won't damage the drill bit. Acetone might also be tried. The other thing that is used to take off organics is plasma cleaning. Even flaming in a Bunsen burner would probably burn off the organics and wouldn't hurt the drill bit if you were quick, followed by alcohol ultrasonic cleaning. The "adhesive" that holds the diamonds is usually sintered cobalt powder and is quite strong. Regards,
-----Original Message----- X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Sent: February 14, 2008 10:51 AM To: maryflet-at-interchange.ubc.ca
Hi, All-
I was recently asked to image some surgical burrs (tiny, diamond-chip-studded drill bits) with SEM. Two were heavily coated with tissue debris. I successfully cleaned one off with chlorine bleach, but the other corroded into an ugly mess. This type of project is outside my range of experience (I tend to avoid clinical stuff). (I know how to get tissue off coral.)
More of these burrs are coming soon. Does anyone have any suggestions for cleaning these burrs while maintaining the integrity of the steel, adhesive, and diamond chips?
Aloha from sunny, warm, etc. Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
Just a quick question. I received new Spurr's resin kits last October and noticed that one of the components is { {very} } much more viscous than the original formula. I had not ordered this in some time.
The kit is supplied by EMS. It is still called a "Spurr's Kit" and lists the original reference of Spurr, 1969 http://www.emsdiasum.com/microscopy/products/embedding/kits.aspx#14300 Orig kit ERL-4206 - 6 cps viscosity - 71g/epoxide equivalent New Kit ERL-4221 - 435 cps viscosity - 137g/epoxide eqiv.
The ERL-4221 is referred to as a direct replacement for the ERL-4206 (not really what I call a direct replacement except that it replaces the other component in the kit.......)
Interestingly, the (new)Techical Data Sheet mentions the viscosities of all the components EXCEPT for the ERL-4221.
I did several tests and found the quality of the cured resin to be noticably different; it seems much more brittle/crumbly - esp. the surface 2 mm of the cast block that are also a bit softer. In thin embedments it was entirely brittle. This is a comparison to blocks from the old mix cured in the same oven at the same time. I suppose the overall property difference could be explained by the "epoxide equivalent" differences (been a while since I had to deal with that!); the data sheet still lists the same grams for each component. The surface layer difference seems to be a different sensitivity to air (O2, moisture?) - the mix went straight into a sealed 70C oven with Drierite. Our lab is very low humidty.
Before I delve into the archives, can someone tell me if there has been discussion of this topic?
Thanks,
Dale Callaham Umass, Amherst, MA, USA
==============================Original Headers============================== 8, 20 -- From dac-at-research.umass.edu Thu Feb 14 13:42:04 2008 8, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EJg4ZZ021697 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 13:42:04 -0600 8, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 20 -- (authenticated bits=0) 8, 20 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1EJg3Wh017899 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 14:42:03 -0500 8, 20 -- Message-ID: {47B49A58.2040707-at-research.umass.edu} 8, 20 -- Date: Thu, 14 Feb 2008 14:45:28 -0500 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 20 -- Reply-To: dac-at-research.umass.edu 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 20 -- Subject: Spurr's Resin - new formula -question 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
You probably should be using an enzymatic cleaner such as is used for cleaning dental or surgical instruments. It is specifically designed to remove organic debris while not corroding the instruments.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Obviously, introducing a CL detector into an EM does not change risk factors associated with it operating under the same conditions as before, unless the mating of the detector to the column is done very poorly (unlikely). The concern about CL being a source of ionizing radiation probably stems from the fact that CL is used in Geology as a stand-alone technique with a light microscope. (often the features of interest are so large that a electron microscope would be superfluous.) In this case, the sample sits inside a shallow, evacuated box with a glass porthole through which it can be viewed while high voltage electrons bombard the surface. Since the operator is typically viewing the sample through a simple microscope while this goes on, he or she is in close proximity to the discharge. The normal construction of such a device takes this into account, with normal shielding to protect from HV electricity and X-rays emitted from the impact of the electron current on the sample. The method has been in use long enough that some CL devices of this type may pre-date current radiation shielding requirements and current attitudes toward minimizing exposure. Another potential problem is that home-built modifications of such a device, for example, to replace a broken sight glass, could have substituted a component of the shielding with something less effective.
Relion Industries' scientist Don Marshall supplies and refurbishes CL devices of several brands (the Luminoscope, etc.)and in my experience has been extremely helpful in providing information about the technique. I'm sure that he has a great deal of information on any safety aspects at hand:
Check out Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations" This article may not address all of your issues, but Ellis put in a lot of good information about the new ERL 4221.
Phil
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==============================Original Headers============================== 4, 22 -- From oshel1pe-at-cmich.edu Thu Feb 14 14:52:58 2008 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1EKqvp6029066 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 14:52:57 -0600 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m1EL8e9N002597 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 16:08:40 -0500 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 22 -- Thu, 14 Feb 2008 15:52:35 -0500 4, 22 -- Mime-Version: 1.0 4, 22 -- Message-Id: {f06240816c3da59bca5d4-at-[141.209.160.249]} 4, 22 -- In-Reply-To: {200802141945.m1EJjhwB028127-at-ns.microscopy.com} 4, 22 -- References: {200802141945.m1EJjhwB028127-at-ns.microscopy.com} 4, 22 -- Date: Thu, 14 Feb 2008 15:52:52 -0500 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 22 -- Subject: Re: [Microscopy] Spurr's Resin - new formula -question 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 22 -- X-OriginalArrivalTime: 14 Feb 2008 20:52:35.0592 (UTC) FILETIME=[86DD0080:01C86F4B] 4, 22 -- X-CanItPRO-Stream: default 4, 22 -- X-Spam-Score: -4 () L_EXCH_MF 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mcintyre-at-optics.rochester.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mcintyre-at-optics.rochester.edu Name: Brian McIntyre
Organization: Univ of Rochester
Title-Subject: [Filtered] JEOL 2000EX w/camera available May/June 08
Question: Hi all-
My workhorse 2000EX has to leave the lab by June. It has been continuously maintained by JEOL and recently had a 4MP bottom mount camera installed (SIA). It works well (graphite lattice with some effort) and would be a good microscope.
Unfortunately it is large and you'd have to cover all the disassembly and shipping costs. In addition the UofR would like about $40K for it (although I'm sure we'd negotiate).
Contact me offline at mcintyre-at-optics.rochester.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marko-at-wadsworth.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Good news for those hoping to submit a paper to Microscopy and Microanalysis 2008! We are extending the deadline from tomorrow to Monday, February 18, at 5PM Pacific Standard Time. Please go to http://bono.cup.org to register, and then submit your paper. The countdown timer has been reset. Please do not forget to approve your paper (usually by visiting the site a final time), once you have submitted it.
Don't miss your chance to participate in world's largest and most exciting microscopy meeting!
See http://mm2008.microscopy.org for more details on the meeting.
Thank you to all who sent information about the "new" Spurr's resin.
Since some replies came off-list, the answer is that this topic has been covered while I was happily working through my old stocks of Spurr's and not paying attention. In summary, there is information in the archives of the list and the formula does need adjusting from the old proportions. The key work on this seems to be the Ellis papers:
Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations"
Corrected Formulation for Spurr Low Viscosity Embedding Medium Using the Replacement Epoxide ERL 4221. Microsc Microanal 12(Supp 2), 2006 E. Ann Ellis Microscopy and Imaging Center, BSBW 119/MS 2257, Texas A&M University, College Station, TX 77843-2257
These instructions also give a new formulation: http://www.2spi.com/catalog/chem/instructions_spurr_kits.html
Again, thank you to all who replied.
Dale Callaham
==============================Original Headers============================== 8, 21 -- From dac-at-research.umass.edu Thu Feb 14 19:37:38 2008 8, 21 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1F1bcnR010703 8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 19:37:38 -0600 8, 21 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 8, 21 -- (authenticated bits=0) 8, 21 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1F1bbvh010309 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 20:37:38 -0500 8, 21 -- Message-ID: {47B4ECED.7050500-at-research.umass.edu} 8, 21 -- Date: Thu, 14 Feb 2008 20:37:49 -0500 8, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Microscopy List {microscopy-at-microscopy.com} 8, 21 -- Subject: Re: [Microscopy] Spurr's Resin - new formula -question ANSWERED, 8, 21 -- THANKS 8, 21 -- References: {3.0.6.32.20080214154534.007bbe20-at-pop3.norton.antivirus} 8, 21 -- In-Reply-To: {3.0.6.32.20080214154534.007bbe20-at-pop3.norton.antivirus} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I work with semiconductor samples, however here are three my favorite reagents for removing occasional organic contamination and debris; all can be used in ultrasonic cleaner. In the order of (personal) preference:
1) Joy dishwasher liquid: http://www.pjpmarketplace.com/servlet/the-1738/Joy%2C-Manual%2C-Dishwashing- Soap%2C/Detail?zmam=9154908&zmas=1&zmac=7&zmap=156041
All give best results if used warm or (if sample allows it) hot, and in combination with DI or distilled water for dilution and rinsing (avoid tap water). Joy seems to be the most "gentle" and Alconox the most "aggressive" of three, however it may be a purely subjective impression...
I have no commercial interest in manufacturers or distributors of these cleaners ;)
Cheers, Valery Ray ============================ www.partbeamsystech.com
-----Original Message----- X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Sent: Thursday, February 14, 2008 1:47 PM To: vray-at-partbeamsystech.com
Hi, All-
I was recently asked to image some surgical burrs (tiny, diamond-chip-studded drill bits) with SEM. Two were heavily coated with tissue debris. I successfully cleaned one off with chlorine bleach, but the other corroded into an ugly mess. This type of project is outside my range of experience (I tend to avoid clinical stuff). (I know how to get tissue off coral.)
More of these burrs are coming soon. Does anyone have any suggestions for cleaning these burrs while maintaining the integrity of the steel, adhesive, and diamond chips?
Aloha from sunny, warm, etc. Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
I'm working on a IC reliability topic and am wondering if anyone on the list has inputs about some of the issues.
The work is to stress IC using HALT/HAST but per JESD22-A110-B. The problem is to identify the amount of bias change per step and at what time points to change the bias. My feeling is that some amount of time is at normal bias, then some time later at normal+10%, then +20% and then 30%. After that, the cycle starts over.
When all of this is done, the EBSD grain data of interconnect metal will be compared to un-stressed grain data. The ICs could be flip chip BGA in plastic packages and/or ceramic. The die are removed from the package and then de-layered using plasma. Interconnects are Copper damascene and also Aluminum damascene and non-damascene.
Any one have any ideas about this? Off-list is fine. All inputs are appreciated.
gary g.
==============================Original Headers============================== 7, 17 -- From gary-at-gaugler.com Fri Feb 15 14:39:50 2008 7, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1FKdobI009118 7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 15 Feb 2008 14:39:50 -0600 7, 17 -- Message-Id: {200802152039.m1FKdobI009118-at-ns.microscopy.com} 7, 17 -- Received: (qmail 13865 invoked from network); 15 Feb 2008 12:39:49 -0800 7, 17 -- Received: by simscan 1.1.0 ppid: 13862, pid: 13863, t: 0.1901s 7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 17 -- by smtp1 with SMTP; 15 Feb 2008 12:39:49 -0800 7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 17 -- Date: Fri, 15 Feb 2008 12:39:47 -0800 7, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 7, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 17 -- Subject: IC stress testing prior to EBSD 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-2C8A5BC5 ==============================End of - Headers==============================
The IM&MF at Emory University has for sale, a DS130 LaB6 SEM. This is a dual stage (in-lens and below lens imaging) scanning electron microscope with a Robinson backscatter detector, all the manuals, & rotory pump. The upper stage has STEM capabilities. Please contact me off list for more information or to give me your best offer.
Thanks, Jeannette Taylor
-- Jeannette Taylor, Technologist II IM&MF, Interim Lab. Manager Cherry L. Emerson Hall, Room E106 Emory University 1515 Dickey Drive Atlanta, Georgia 30322
SDD detectors and pulse processors are really coming on strong. The newest generation of these are impressive. There is an issue I think with SDDs that has not been discussed. Or, I am missing something?
A Si(Li) or SDD will do a nice job of collecting spectra of elements with good resolution (~129eV) when cps and DT are low with longest filter time. Not a problem. However, the resolution of EDS degrades as filter time is decreased. This is true for both types of detectors. The advertised ability of SDDs to collect at high cps is true if the filter time is reduced to keep DT low.
So, suppose one is collecting a string of light elements--C, N, O, F, Na, Al and Si. Either detector can do this, if at highest resolution-- meaning, longest filter time. Now suppose the task is to do a fast map of these elements. Increasing cps via normal means causes DT to increase and thus requires shorter filter time. As this happens, resolution degrades to a point at some higher cps where the peaks are not individually resolved. The result is a useless map.
Thus, the claim of high cps ability is true on one hand but not across the board for spectra and mapping. Yes??
For Si(Li) and it seems for SDD mapping, one must create two element lists such that there is sufficient eV resolution between collected elements to result in meaningful maps. An example might be C, O, Na and Si as list one. List two would then be N, F, Al. It might be necessary to have three lists so that one could take advantage of higher cps and shorter mapping time. This would also help in drift correction. Perhaps there are other methods or details I am missing?
Off-list discussion regarding maker-specific stuff ought to be the MO in this case. General feedback via any method is appreciated as well.
gary g.
==============================Original Headers============================== 10, 17 -- From gary-at-gaugler.com Fri Feb 15 15:09:00 2008 10, 17 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1FL8wHK031618 10, 17 -- for {microscopy-at-microscopy.com} ; Fri, 15 Feb 2008 15:08:58 -0600 10, 17 -- Message-Id: {200802152108.m1FL8wHK031618-at-ns.microscopy.com} 10, 17 -- Received: (qmail 16475 invoked from network); 15 Feb 2008 13:08:41 -0800 10, 17 -- Received: by simscan 1.1.0 ppid: 16472, pid: 16473, t: 0.0747s 10, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 17 -- by qsmtp4 with SMTP; 15 Feb 2008 13:08:40 -0800 10, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 17 -- Date: Fri, 15 Feb 2008 13:08:55 -0800 10, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 10, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 17 -- Subject: SDD element mapping 10, 17 -- Mime-Version: 1.0 10, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-2C8A5BC5 ==============================End of - Headers==============================
gary-at-gaugler.com wrote: } } } Thus, the claim of high cps ability is true on } one hand but not across the board for spectra } and mapping. Yes?? }
Not quite. SDDs routinely get good resolution with processing times around 1/10 to 1/20 of the Si(Li) processing times required for best resolution.
So depending on your definition of "high", it is perfectly reasonable to run the same processing time always with an SDD. You can get to 30kcps easily, which would be screaming with a Si(Li), and keep the resolution around 130 eV. In many cases, you won't generate more than that if you want to keep the spatial resolution good. (Yes, this depends on the particular SDD/processor combo you have. They aren't all the same, and there have been generation changes just in the last year or so. Bruker's ads are currently claiming 125 eV *at 100kcps*.)
But who are we kidding here? Not so long ago 133 eV was considered great. Anything in the mid-130's is good enough to separate C, N, O and F nicely. Low 130's to mid 120's even splits the Ll lines from the L-alphas for the transition metals, which is awesome but probably not required for mapping.
There's another key point, which relates to Stephane's query of a few days ago (to which I will reply separately). The dead time will be much lower with SDDs than with Si(Li)'s, again because of the shorter processing time, which means you collect more of the X-rays you generate. At 30kcps and 130 eV or so, you can stay down around 10-15% dead time. That's a big win.
Pile-up (sum peaks) matters for the list of elements you describe as the rates go up, and their relative intensities are proportional to the *input* count rate, not throughput rate. 100 kcps at 50% DT is six times the input rate of 30kcps at 10% DT, so all other things being equal, the sum peaks will be 6x bigger in relative terms, not 3x. The O sum peak is within 9 eV of the Na K, for example. So it's a bit risky to try for the highest rates touted for the SDD, if you have significant peaks below 1 keV in the spectrum. If you're only mapping major elements, you can mostly ignore the pile-up, but if you want to pick up minor elements it can get you in trouble, especially if you want to try anything resembling "quantitative mapping".
Rick Mott, official pulse processor wizard for PulseTor
(Disclaimer: PulseTor makes SDDs and processors, so we have an obvious interest in promoting SDDs)
==============================Original Headers============================== 9, 19 -- From rmott-at-pulsetor.com Fri Feb 15 16:34:43 2008 9, 19 -- Received: from smtpout10.prod.mesa1.secureserver.net (smtpout10-04.prod.mesa1.secureserver.net [64.202.165.238]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1FMYfHW020613 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 15 Feb 2008 16:34:42 -0600 9, 19 -- Received: (qmail 559 invoked from network); 15 Feb 2008 22:34:38 -0000 9, 19 -- Received: from unknown (24.149.176.235) 9, 19 -- by smtpout10-04.prod.mesa1.secureserver.net (64.202.165.238) with ESMTP; 15 Feb 2008 22:34:37 -0000 9, 19 -- Message-ID: {47B6138A.804-at-pulsetor.com} 9, 19 -- Date: Fri, 15 Feb 2008 17:34:50 -0500 9, 19 -- From: Rick Mott {rmott-at-pulsetor.com} 9, 19 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.7.8) Gecko/20050511 9, 19 -- X-Accept-Language: en-us, en 9, 19 -- MIME-Version: 1.0 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- Subject: Re: [Microscopy] SDD element mapping 9, 19 -- References: {200802152116.m1FLGKMi017456-at-ns.microscopy.com} 9, 19 -- In-Reply-To: {200802152116.m1FLGKMi017456-at-ns.microscopy.com} 9, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Please could you advise me of the best way to produce false-colour images of black & white digital SEM captures.
Is there specific software for this, or is it generally done in somethig like Corel PhotoPaint (which I have experimented with).
I'd be really grateful for any advice.
Many thanks,
Anthony.
_________________________________
Dr Anthony Butcher Palynological Research School of Earth & Environmental Sciences University of Portsmouth Burnaby Building Burnaby Road Portsmouth PO1 3QL United kingdom Tel: (+44) 23 9284 2258 Fax: (+44) 23 9284 2244 anthony.butcher-at-port.ac.uk _________________________________
==============================Original Headers============================== 12, 22 -- From Anthony.Butcher-at-port.ac.uk Sat Feb 16 05:50:41 2008 12, 22 -- Received: from rioja.iso.port.ac.uk (rioja.iso.port.ac.uk [148.197.254.4]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1GBoeVi022871 12, 22 -- for {Microscopy-at-Microscopy.Com} ; Sat, 16 Feb 2008 05:50:41 -0600 12, 22 -- Received: from stirling.iso.port.ac.uk ([148.197.251.204]:35177) 12, 22 -- by rioja.iso.port.ac.uk with esmtp (Exim 4.66) 12, 22 -- (envelope-from {Anthony.Butcher-at-port.ac.uk} ) 12, 22 -- id 1JQLZ1-0001Zw-Oc 12, 22 -- for Microscopy-at-Microscopy.Com; Sat, 16 Feb 2008 11:50:39 +0000 12, 22 -- Received: from gwia2dm-MTA by stirling.iso.port.ac.uk 12, 22 -- with Novell_GroupWise; Sat, 16 Feb 2008 11:50:39 +0000 12, 22 -- Message-Id: {47B6CE08020000750004FA72-at-stirling.iso.port.ac.uk} 12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 12, 22 -- Date: Sat, 16 Feb 2008 11:50:31 +0000 12, 22 -- From: "Anthony Butcher" {Anthony.Butcher-at-port.ac.uk} 12, 22 -- To: {Microscopy-at-Microscopy.Com} 12, 22 -- Subject: False-colouring of SEM images 12, 22 -- Mime-Version: 1.0 12, 22 -- Content-Type: text/plain; charset=US-ASCII 12, 22 -- Content-Transfer-Encoding: 7bit 12, 22 -- Content-Disposition: inline 12, 22 -- X-Scan-Signature: d7130811212804196524fdc60755bb8d ==============================End of - Headers==============================
Dear Anthony, colouring images depends on various things and can be easier or harder to do...
The easiest way is having not one but two or more detectors on the SEM to use during aquiring an image. These different b&w images can be converted to RGB files with Photoshop or any other image editing software (ImageJ, ...), attributed with a chosen colour and mixed. This will give you a two- or more (numbers of detectors) coloured image, including all the mixed colours of the colours chosen for each b&w image. Most of the images on the following sites are made with only two SE / BSE detectors: www.elektronenmikroskopie.info/ausstellungen/wuerzburg (please give the site some seconds to load and then do right clicks to see further images) and www.quantifoil.com/calendar_2008.pdf Sometimes I used paths to alter parts of the image in colour (background etc.)
Normal way would be having only one b&w image to start with. Here you need to do a lot of work, basically starting with converting to RGB and changing to a colour fitting your specimen. Next you need to manipulate parts of the image using paths or masks ( I am accustomed to Photoshop, but I think any other editing software where you can work very precisely with paths, masks, etc will do it...) in colour and tonality. This will mostly lead to a more "artificial" re-inventing the specimen you had as a b&w image. Normally - unless you work very precisely - it will not look natural.
So: best would be to use multiple detectors and a scanning electronic like DISS5 from www.pointelectronic.de (...only satisfied customer...), which enables you to use max. 4 detectors parallel, use one detector for the basic colour, the other to use as coloured spot-lights...
----- Original Message ----- X-from: {Anthony.Butcher-at-port.ac.uk} To: {stefan.diller-at-t-online.de} Sent: Saturday, February 16, 2008 12:57 PM
Hi Anthony,
It is not clear to me what you mean by false coloring. Color assignment is done for a variety of reasons. If you mean the way people show a bacterium as {shades of green} in an otherwise grayscale image, better to let others help you with artwork. But keep in mind that this is artwork and please see the Rossner and Yamada paper (link at the end)....
SEM images are inherently just intensity data - a binary value representing the intensity of "signal" for each pixel position. This is traditionally "monochrome" or grayscale but color can be assigned to represent each intensity as well. It is easy with ImageJ (http://rsb.info.nih.gov/ij/)to assign a LUT to a grayscale image. This does not change any values, just substitutes colors for the grayscale value. To the extent that the colors or color mapping may not be, or be perceived as, linear intensity, this can be deceptive if used improperly.
As Stefan Diller pointed out, if you have different detectors and thus different information, these different views can be used to map an image in color, distinguishing the features revealed by those detectors.
Another reason for using color in SEM images is to show depth - if one (grayscale) image is taken and then the sample is tilted some degrees and another is taken, these images can be assigned to the red and green (or red and blue) channels of a RGB image. Depending on the software used, it either uses zero values for the blue channel, or an image of zero must be supplied for the empty channel. The RGB image, viewed through R-G (or R-B) glasses allows each eye to see only one view and the brain "sees" the depth.
If you are getting started with this, the link for Digital Imaging at the Molecular Expressions website is a good place to start for reading on digital imaging (including manipulations): http://micro.magnet.fsu.edu/primer/digitalimaging/index.html
But also { {very important} } reading (if the result is for publication) is the Rossner and Yamada paper in JCB (available as pdf): http://www.jcb.org/cgi/content/full/166/1/11
Cheers!
Dale
} } } ----- Original Message ----- } X-from: {Anthony.Butcher-at-port.ac.uk} } To: {stefan.diller-at-t-online.de} } Sent: Saturday, February 16, 2008 12:57 PM } Subject: [Microscopy] False-colouring of SEM images } } } } } } } } -------------------------------------------------------------------------- } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } -- } } Dear all, } } } } Please could you advise me of the best way to produce false-colour } } images of black & white digital SEM captures. } } } } Is there specific software for this, or is it generally done in somethig } } like Corel PhotoPaint (which I have experimented with). } } } } I'd be really grateful for any advice. } } } } Many thanks, } } } } Anthony. } } } } } } } } } } } } _________________________________ } } } } Dr Anthony Butcher } } Palynological Research } } School of Earth & Environmental Sciences } } University of Portsmouth } } Burnaby Building } } Burnaby Road } } Portsmouth } } PO1 3QL } } United kingdom } } Tel: (+44) 23 9284 2258 } } Fax: (+44) 23 9284 2244 } } anthony.butcher-at-port.ac.uk } } _________________________________ } } } } ==============================Original } Headers============================== } } 12, 22 -- From Anthony.Butcher-at-port.ac.uk Sat Feb 16 05:50:41 2008 } } 12, 22 -- Received: from rioja.iso.port.ac.uk (rioja.iso.port.ac.uk } [148.197.254.4]) } } 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m1GBoeVi022871 } } 12, 22 -- for {Microscopy-at-Microscopy.Com} ; Sat, 16 Feb 2008 05:50:41 -0600 } } 12, 22 -- Received: from stirling.iso.port.ac.uk ([148.197.251.204]:35177) } } 12, 22 -- by rioja.iso.port.ac.uk with esmtp (Exim 4.66) } } 12, 22 -- (envelope-from {Anthony.Butcher-at-port.ac.uk} ) } } 12, 22 -- id 1JQLZ1-0001Zw-Oc } } 12, 22 -- for Microscopy-at-Microscopy.Com; Sat, 16 Feb 2008 11:50:39 +0000 } } 12, 22 -- Received: from gwia2dm-MTA by stirling.iso.port.ac.uk } } 12, 22 -- with Novell_GroupWise; Sat, 16 Feb 2008 11:50:39 +0000 } } 12, 22 -- Message-Id: {47B6CE08020000750004FA72-at-stirling.iso.port.ac.uk} } } 12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 } } 12, 22 -- Date: Sat, 16 Feb 2008 11:50:31 +0000 } } 12, 22 -- From: "Anthony Butcher" {Anthony.Butcher-at-port.ac.uk} } } 12, 22 -- To: {Microscopy-at-Microscopy.Com} } } 12, 22 -- Subject: False-colouring of SEM images } } 12, 22 -- Mime-Version: 1.0 } } 12, 22 -- Content-Type: text/plain; charset=US-ASCII } } 12, 22 -- Content-Transfer-Encoding: 7bit } } 12, 22 -- Content-Disposition: inline } } 12, 22 -- X-Scan-Signature: d7130811212804196524fdc60755bb8d } } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 16, 25 -- From stefan.diller-at-t-online.de Sat Feb 16 07:54:01 2008 } 16, 25 -- Received: from mailout01.sul.t-online.com (mailout01.sul.t-online.de [194.25.134.80]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1GDrx6r006542 } 16, 25 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 07:54:00 -0600 } 16, 25 -- Received: from fwd26.aul.t-online.de } 16, 25 -- by mailout01.sul.t-online.com with smtp } 16, 25 -- id 1JQNUG-0003yR-02; Sat, 16 Feb 2008 14:53:52 +0100 } 16, 25 -- Received: from dose (VyqVLwZVZhKsUo6+Zl5OlT7GxEBnyMBxzUh1+o4AED6PguEeaubpRgbCibvCztbZTy-at-[91.10.218.248]) by fwd26.aul.t-online.de } 16, 25 -- with smtp id 1JQNTz-0ZSCxs0; Sat, 16 Feb 2008 14:53:35 +0100 } 16, 25 -- Message-ID: {001f01c870a3$5482d660$0f02a8c0-at-dose} } 16, 25 -- From: "Stefan Diller" {stefan.diller-at-t-online.de} } 16, 25 -- To: {microscopy-at-microscopy.com} } 16, 25 -- References: {200802161157.m1GBvCjt030630-at-ns.microscopy.com} } 16, 25 -- Subject: Re: [Microscopy] False-colouring of SEM images } 16, 25 -- Date: Sat, 16 Feb 2008 14:53:36 +0100 } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- Content-Type: text/plain; } 16, 25 -- charset="iso-8859-1" } 16, 25 -- Content-Transfer-Encoding: 7bit } 16, 25 -- X-Priority: 3 } 16, 25 -- X-MSMail-Priority: Normal } 16, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1914 } 16, 25 -- X-Mimeole: Produced By Microsoft MimeOLE V6.00.2800.1914 } 16, 25 -- X-ID: VyqVLwZVZhKsUo6+Zl5OlT7GxEBnyMBxzUh1+o4AED6PguEeaubpRgbCibvCztbZTy } 16, 25 -- X-TOI-MSGID: 877bc58a-bd03-46b2-8b34-5562a97f403e } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 20 -- From dac-at-research.umass.edu Sat Feb 16 10:03:55 2008 12, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1GG3toX028892 12, 20 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 10:03:55 -0600 12, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 12, 20 -- (authenticated bits=0) 12, 20 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1GG3rsv022216 12, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 12, 20 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 11:03:54 -0500 12, 20 -- Message-ID: {47B70979.8020100-at-research.umass.edu} 12, 20 -- Date: Sat, 16 Feb 2008 11:04:09 -0500 12, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 12, 20 -- MIME-Version: 1.0 12, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 12, 20 -- Subject: Re: [Microscopy] Re: False-colouring of SEM images 12, 20 -- References: {200802161403.m1GE3VY6015559-at-ns.microscopy.com} 12, 20 -- In-Reply-To: {200802161403.m1GE3VY6015559-at-ns.microscopy.com} 12, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On re-reading yesterday's remarks, I realized a clarification/apology is in order.
Improved resolution is always good, and I did not intend to downplay the achievement represented by Bruker's results, which are unmatched in the industry. The best SDDs are now reaching best resolutions which can't be obtained with Si(Li) at all.
With any system, though, potential pile-up at low energies can be minimized by selecting an intermediate shaping time and accepting a small loss of resolution in return for a significant reduction in input count rate, by lowering the dead time. The resolution change for an SDD is (generally) much less with processing time than for a Si(Li), so giving up a few eV can get you a 30-50% reduction in sum peaks.
That's not the same as saying resolution doesn't matter, which is how my previous post came across. Sorry...
Rick Mott, PulseTor LLC
==============================Original Headers============================== 9, 19 -- From rmott-at-pulsetor.com Sat Feb 16 10:48:50 2008 9, 19 -- Received: from smtpout09.prod.mesa1.secureserver.net (smtpout09-04.prod.mesa1.secureserver.net [64.202.165.17]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1GGmnnQ010562 9, 19 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 10:48:49 -0600 9, 19 -- Received: (qmail 11633 invoked from network); 16 Feb 2008 16:48:47 -0000 9, 19 -- Received: from unknown (24.149.176.235) 9, 19 -- by smtpout09-04.prod.mesa1.secureserver.net (64.202.165.17) with ESMTP; 16 Feb 2008 16:48:46 -0000 9, 19 -- Message-ID: {47B713F5.2070903-at-pulsetor.com} 9, 19 -- Date: Sat, 16 Feb 2008 11:48:53 -0500 9, 19 -- From: Rick Mott {rmott-at-pulsetor.com} 9, 19 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.7.8) Gecko/20050511 9, 19 -- X-Accept-Language: en-us, en 9, 19 -- MIME-Version: 1.0 9, 19 -- CC: microscopy-at-microscopy.com 9, 19 -- Subject: Re: [Microscopy] SDD element mapping 9, 19 -- References: {200802152116.m1FLGKMi017456-at-ns.microscopy.com} {47B6138A.804-at-pulsetor.com} 9, 19 -- In-Reply-To: {47B6138A.804-at-pulsetor.com} 9, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was curious when a filament starts to degrade if you see the effect larger at high or low kV. We have an ESEM that we are still trying to learn taking wet samples. At 5 kV to attempt less damage to the cells I can't seem to get a focus to clearly distinguish features less than 1mm. Then I tested imaging a normal sample under high vac with the low kV and still couldn't get a good focus on the sample. I don't remember ever using that low of a kV in high vac before but I thought even at 5kV you should be able to get a better focus than what was acheived, and at 30kV I was still able to get a clear crisp image on the order of 1 micron-100nm. Articles pulled from a few journals show other users with similar scopes getting fairly clean images on the scale of 10 microns. Is it possible I am doing something wrong or might the filament just being aging, it has been in the scope for 46 hours and our normal lifetime seems to be around 50 hours. Thanks.
-Jason Saredy University of North Florida
==============================Original Headers============================== 2, 27 -- From sarj0007-at-unf.edu Sat Feb 16 12:11:03 2008 2, 27 -- Received: from relay2.unf.edu (smarthost2.unf.edu [139.62.192.129]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1GIB3Lq026119 2, 27 -- for {Microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 12:11:03 -0600 2, 27 -- X-IronPort-Anti-Spam-Filtered: true 2, 27 -- X-IronPort-Anti-Spam-Result: Ao8CAD+2tkeLPsjX/2dsb2JhbACsWw 2, 27 -- Received: from crystal3.unfcsd.unf.edu ([139.62.200.215]) 2, 27 -- by relay2.unf.edu with ESMTP; 16 Feb 2008 13:11:01 -0500 2, 27 -- Received: from emerald.unfcsd.unf.edu ([139.62.200.208]) by CRYSTAL3.unfcsd.unf.edu with Microsoft SMTPSVC(6.0.3790.1830); 2, 27 -- Sat, 16 Feb 2008 13:11:03 -0500 2, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 2, 27 -- Content-class: urn:content-classes:message 2, 27 -- MIME-Version: 1.0 2, 27 -- Content-Type: text/plain; 2, 27 -- charset="iso-8859-1" 2, 27 -- Subject: W Filament and ESEM 2, 27 -- Date: Sat, 16 Feb 2008 13:11:02 -0500 2, 27 -- Message-ID: {D80383FB8CD3474B81CA3D67F248622901EBDCDC-at-EMERALD.unfcsd.unf.edu} 2, 27 -- X-MS-Has-Attach: 2, 27 -- X-MS-TNEF-Correlator: 2, 27 -- Thread-Topic: W Filament and ESEM 2, 27 -- Thread-Index: Achwx0ox1OfZgTG0SLmIF7LmKJkydw== 2, 27 -- From: "Saredy, Jason" {sarj0007-at-unf.edu} 2, 27 -- To: {Microscopy-at-microscopy.com} 2, 27 -- X-OriginalArrivalTime: 16 Feb 2008 18:11:03.0212 (UTC) FILETIME=[4A94AEC0:01C870C7] 2, 27 -- Content-Transfer-Encoding: 8bit 2, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1GIB3Lq026119 ==============================End of - Headers==============================
I've checked numbers and find that I'm probably wrong about comparisons between Si(Li) and SDD.
With a digital pulse processor and 10mm^2 Si(Li) detector, the resolution versus filter times are:
uS eV 102 131 51 132 25 141 12 161 6 172 3 225 1.6 257
and for 40mm^2 SDD:
uS eV 25 131 12 133 6 134 3 144 1.6 158 0.8 167
Both detectors are with ultra thin Moxtec polymer window. The resolution numbers are after calibration with Cu/Al peaks and 30% DT or less.
So it seems that the SDD has very good resolution across the time range compared to Si(Li). Thus, the main difference with filter time is to get DT down to 30% or less at whatever cps are reported. It then does not seem that filter time is going to adversely affect peak discrimination for mapping. If only alpha peaks are used, then pile up ought not be an issue either.
Guess I need to do more work with the SDD and different specimens and conditions.
gary g.
At 01:10 PM 2/15/2008, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 19 -- From gary-at-gaugler.com Sat Feb 16 15:59:40 2008 13, 19 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1GLxdiH014882 13, 19 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 15:59:40 -0600 13, 19 -- Message-Id: {200802162159.m1GLxdiH014882-at-ns.microscopy.com} 13, 19 -- Received: (qmail 1475 invoked from network); 16 Feb 2008 13:59:38 -0800 13, 19 -- Received: by simscan 1.1.0 ppid: 1472, pid: 1473, t: 0.1001s 13, 19 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 19 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 13, 19 -- by smtp1 with SMTP; 16 Feb 2008 13:59:38 -0800 13, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 19 -- Date: Sat, 16 Feb 2008 13:59:35 -0800 13, 19 -- To: MSA listserver {microscopy-at-microscopy.com} 13, 19 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 19 -- Subject: Re: [Microscopy] SDD element mapping 13, 19 -- In-Reply-To: {200802152110.m1FLAq6s004415-at-ns.microscopy.com} 13, 19 -- References: {200802152110.m1FLAq6s004415-at-ns.microscopy.com} 13, 19 -- Mime-Version: 1.0 13, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-1B7B2848 ==============================End of - Headers==============================
Some call false color "pseudo color." Either way, it is the conversion of a grey scale image to color.
The process can be done by combining color separations as b/w images from separate detectors or by using a look up table (LUT) for grey scale values to color. Combining detector images can be done using Dindima Spectrum while complex LUTs can be created with Maxim DL. Alternatively, one can use Photoshop to create layers with different colors of accentuation based on grey scale intensity.
The LUT methods typically require a post processing noise filter like Gaussian or median. The multiple detector images do not. If you take one image with one detector and another image with another detector, they likely will not be exactly aligned. An FFT alignment routine will bring them together. SIS analySIS Opti or Fovea Pro will do this. The interactive deconvolution feature of Fovea Pro is also handy to tweak final definition of image files that need this.
Some examples of software colorization of SEM pix can be seen at:
Some of these pix are somewhat aggressively compressed and display artifacts of compression. Try to ignore this.
gary g.
At 03:52 AM 2/16/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Sat Feb 16 19:49:28 2008 11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1H1nSTg001423 11, 20 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 19:49:28 -0600 11, 20 -- Message-Id: {200802170149.m1H1nSTg001423-at-ns.microscopy.com} 11, 20 -- Received: (qmail 11199 invoked from network); 16 Feb 2008 17:49:27 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 11195, pid: 11196, t: 0.1125s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp1 with SMTP; 16 Feb 2008 17:49:27 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Sat, 16 Feb 2008 17:49:24 -0800 11, 20 -- To: Anthony.Butcher-at-port.ac.uk 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] False-colouring of SEM images 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200802161152.m1GBqt8D025548-at-ns.microscopy.com} 11, 20 -- References: {200802161152.m1GBqt8D025548-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3B4B185A ==============================End of - Headers==============================
Although it is true that a Look Up Table (LUT) matches intensity values with color and intensity, one can:
1) selectively either modify the image first then apply an LUT (Windows and Mac have standard LUTs as do most software)
or
2) in certain programs (Photoshop is a good example), one can "paint" or "bucket" specific colors within certain boundaries or overlay certain colors in layers
Or
3) use one color that matches gray scale intensity to color intensity or a color intensity with boundaries (Example would be Fovea's plug in for Photoshop with thresholding conversions)
Or
4) Apply (more advanced) functions of color and intensity to the gray scale intensity (Ex. Using Matlab or Mathematica programming).
Any names used are not necessarily endorsements just convenient examples. I have no financial interest in any of those listed.
John Russ, on this listserve may have additional ideas.
The question to ask first is why do it? Always know why before doing it The second question is how can it be done in consideration of human factors (contrast, edge directions, frequency detection, color schemes, etc.)?
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
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-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Saturday, February 16, 2008 8:56 PM To: ph2-at-sprynet.com
Some call false color "pseudo color." Either way, it is the conversion of a grey scale image to color.
The process can be done by combining color separations as b/w images from separate detectors or by using a look up table (LUT) for grey scale values to color. Combining detector images can be done using Dindima Spectrum while complex LUTs can be created with Maxim DL. Alternatively, one can use Photoshop to create layers with different colors of accentuation based on grey scale intensity.
The LUT methods typically require a post processing noise filter like Gaussian or median. The multiple detector images do not. If you take one image with one detector and another image with another detector, they likely will not be exactly aligned. An FFT alignment routine will bring them together. SIS analySIS Opti or Fovea Pro will do this. The interactive deconvolution feature of Fovea Pro is also handy to tweak final definition of image files that need this.
Some examples of software colorization of SEM pix can be seen at:
Some of these pix are somewhat aggressively compressed and display artifacts of compression. Try to ignore this.
gary g.
At 03:52 AM 2/16/2008, you wrote: } --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Sat Feb 16 19:49:28 2008 11, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1H1nSTg001423 11, 20 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 19:49:28 -0600 11, 20 -- Message-Id: {200802170149.m1H1nSTg001423-at-ns.microscopy.com} 11, 20 -- Received: (qmail 11199 invoked from network); 16 Feb 2008 17:49:27 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 11195, pid: 11196, t: 0.1125s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp1 with SMTP; 16 Feb 2008 17:49:27 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Sat, 16 Feb 2008 17:49:24 -0800 11, 20 -- To: Anthony.Butcher-at-port.ac.uk 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] False-colouring of SEM images 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200802161152.m1GBqt8D025548-at-ns.microscopy.com} 11, 20 -- References: {200802161152.m1GBqt8D025548-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3B4B185A ==============================End of - Headers==============================
==============================Original Headers============================== 35, 26 -- From ph2-at-sprynet.com Sat Feb 16 20:10:01 2008 35, 26 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 35, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1H2A16x014092 35, 26 -- for {microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 20:10:01 -0600 35, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 35, 26 -- s=dk20050327; d=sprynet.com; 35, 26 -- b=Mh5Z3oNWvBbqMSDi4ZldY1Ti/LgUOmvoGf4TmUoCk3USkRRUSjDlrDhKsfBwp24m; 35, 26 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:Thread-Index:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 35, 26 -- Received: from [68.73.159.67] (helo=user915fa8f284) 35, 26 -- by elasmtp-dupuy.atl.sa.earthlink.net with asmtp (Exim 4.34) 35, 26 -- id 1JQYye-0004lR-41; Sat, 16 Feb 2008 21:10:00 -0500 35, 26 -- From: "Tony Havics" {ph2-at-sprynet.com} 35, 26 -- To: {gary-at-gaugler.com} , "Microscopy Listserve" {microscopy-at-microscopy.com} 35, 26 -- Subject: RE: [Microscopy] Re: False-colouring of SEM images 35, 26 -- Date: Sat, 16 Feb 2008 21:09:57 -0500 35, 26 -- MIME-Version: 1.0 35, 26 -- Content-Type: text/plain; 35, 26 -- charset="us-ascii" 35, 26 -- Content-Transfer-Encoding: 7bit 35, 26 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 35, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 35, 26 -- Thread-Index: AchxCDTTheT133mhSjO4ItgWYJ7RWAAABZtQ 35, 26 -- In-Reply-To: {200802170155.m1H1te2N009311-at-ns.microscopy.com} 35, 26 -- Message-ID: {E1JQYye-0004lR-41-at-elasmtp-dupuy.atl.sa.earthlink.net} 35, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f95a91b4b5d2c41c9d1a8d100a2754e1f4350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 35, 26 -- X-Originating-IP: 68.73.159.67 ==============================End of - Headers==============================
A colleague is looking for an in-situ FIB/TEM instrument to look at a couple of sample. He needs that the sample does not leave vacuum after FIB preparation. His thesis supervisor and him will prefer a location in Canada or close to McGill Univeristy (Montreal), but other location in North America would be considered.
I don't have a lot of details on the sample, but if you think that other technique can be used than an in-situ FIB/TEM I will transfer the information to my colleague.
Thanks, Hendrix
----------------------------------------------------------- Hendrix Demers Ph.D student ----------------------------------------------------------- Mining and Materials Engineering McGill University Wong building, room 2360 3610 University street Montreal, Quebec H3A 2B2 Ph. : 514-398-4755 #09511 hendrix.demers-at-mail.mcgill.ca -----------------------------------------------------------
==============================Original Headers============================== 5, 27 -- From drix00-at-gmail.com Mon Feb 18 11:41:52 2008 5, 27 -- Received: from hs-out-0708.google.com (hs-out-0708.google.com [64.233.178.244]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1IHfqM0021006 5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 11:41:52 -0600 5, 27 -- Received: by hs-out-0708.google.com with SMTP id 55so2626689hsc.2 5, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 09:41:51 -0800 (PST) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=WGduNT1vut0y5+M6i17eNI6FGDcMxS1Qh0Lguoq7Eow=; 5, 27 -- b=dHJU7buo8V/NHRGqSeOtd9V/jCT/hwulAr/ARkDpjRq6huLumMaKJfEHHJE+ljuaxxK+AatBzJJ4ZepyzL12tYQ7FvEVUipC4NCe7BfJyM4L1PYt80t2AyxCNvCzzSyx7Eo/t62u65XboeTQT3yOlv/qQXW7ShqGyCk4hRc2OnQ= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=bRt4btXScXImXs1mn+8cfJ4qSUC7mR6vI2KdMoR5BpowgAX/+qlITrQ/xhTd/kFDVGMzE/Cf+kuePfgarJYACxirFLc5825uGCBbirOyh/WGGm0XbNMpTo2bTBuRRhIvExw0NbNsjFDnAXjfNC/4279P5DYHdviorWYS3Q/KJmM= 5, 27 -- Received: by 10.100.154.9 with SMTP id b9mr11919082ane.70.1203356511286; 5, 27 -- Mon, 18 Feb 2008 09:41:51 -0800 (PST) 5, 27 -- Received: by 10.100.91.18 with HTTP; Mon, 18 Feb 2008 09:41:51 -0800 (PST) 5, 27 -- Message-ID: {a779eeae0802180941x7b5a2d33r557a06aa0f97be41-at-mail.gmail.com} 5, 27 -- Date: Mon, 18 Feb 2008 12:41:51 -0500 5, 27 -- From: drix {drix00-at-gmail.com} 5, 27 -- To: Microscopy-at-microscopy.com 5, 27 -- Subject: Looking for an in-situ FIB/TEM instrument in Canada or East of North America 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Just as an update. Yes the aperatures were replaced recently, the normal procedures for focusing are known on this end, and maintence as well. The normal sample refered to in the original post refered to a TEM grid with gold flakes. Thanks for suggesting resaturating the filament at the lower kV. Readjusting some things this morning it was noted how the stigmator didn't appear to be affecting the image until it was placed to an extreme. The images started to come in decently under high vac, but it leaves no room for fine tuning of the stigmator. So alignment problems perhaps with the lenses? And I am fairly certain we are not oversaturating the filament, but several members of the listserv commented our filament lifetime was much shorter than normal. Thanks for all the quick replies.
-Jason Saredy University of North Florida
________________________________
X-from: sarj0007-at-unf.edu [mailto:sarj0007-at-unf.edu] Sent: Sat 2/16/2008 1:15 PM To: Saredy, Jason
I was curious when a filament starts to degrade if you see the effect larger at high or low kV. We have an ESEM that we are still trying to learn taking wet samples. At 5 kV to attempt less damage to the cells I can't seem to get a focus to clearly distinguish features less than 1mm. Then I tested imaging a normal sample under high vac with the low kV and still couldn't get a good focus on the sample. I don't remember ever using that low of a kV in high vac before but I thought even at 5kV you should be able to get a better focus than what was acheived, and at 30kV I was still able to get a clear crisp image on the order of 1 micron-100nm. Articles pulled from a few journals show other users with similar scopes getting fairly clean images on the scale of 10 microns. Is it possible I am doing something wrong or might the filament just being aging, it has been in the scope for 46 hours and our normal lifetime seems to be around 50 hours. Thanks.
-Jason Saredy University of North Florida
==============================Original Headers============================== 2, 27 -- From sarj0007-at-unf.edu Sat Feb 16 12:11:03 2008 2, 27 -- Received: from relay2.unf.edu (smarthost2.unf.edu [139.62.192.129]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1GIB3Lq026119 2, 27 -- for {Microscopy-at-microscopy.com} ; Sat, 16 Feb 2008 12:11:03 -0600 2, 27 -- X-IronPort-Anti-Spam-Filtered: true 2, 27 -- X-IronPort-Anti-Spam-Result: Ao8CAD+2tkeLPsjX/2dsb2JhbACsWw 2, 27 -- Received: from crystal3.unfcsd.unf.edu ([139.62.200.215]) 2, 27 -- by relay2.unf.edu with ESMTP; 16 Feb 2008 13:11:01 -0500 2, 27 -- Received: from emerald.unfcsd.unf.edu ([139.62.200.208]) by CRYSTAL3.unfcsd.unf.edu with Microsoft SMTPSVC(6.0.3790.1830); 2, 27 -- Sat, 16 Feb 2008 13:11:03 -0500 2, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 2, 27 -- Content-class: urn:content-classes:message 2, 27 -- MIME-Version: 1.0 2, 27 -- Content-Type: text/plain; 2, 27 -- charset="iso-8859-1" 2, 27 -- Subject: W Filament and ESEM 2, 27 -- Date: Sat, 16 Feb 2008 13:11:02 -0500 2, 27 -- Message-ID: {D80383FB8CD3474B81CA3D67F248622901EBDCDC-at-EMERALD.unfcsd.unf.edu} 2, 27 -- X-MS-Has-Attach: 2, 27 -- X-MS-TNEF-Correlator: 2, 27 -- Thread-Topic: W Filament and ESEM 2, 27 -- Thread-Index: Achwx0ox1OfZgTG0SLmIF7LmKJkydw== 2, 27 -- From: "Saredy, Jason" {sarj0007-at-unf.edu} 2, 27 -- To: {Microscopy-at-microscopy.com} 2, 27 -- X-OriginalArrivalTime: 16 Feb 2008 18:11:03.0212 (UTC) FILETIME=[4A94AEC0:01C870C7] 2, 27 -- Content-Transfer-Encoding: 8bit 2, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1GIB3Lq026119 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 28 -- From sarj0007-at-unf.edu Mon Feb 18 12:19:59 2008 16, 28 -- Received: from relay1.unf.edu (relay1.unf.edu [139.62.192.128]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1IIJxhf002722 16, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 12:19:59 -0600 16, 28 -- X-IronPort-Anti-Spam-Filtered: true 16, 28 -- X-IronPort-Anti-Spam-Result: Ao8CAKdauUeLPsjW/2dsb2JhbACtBg 16, 28 -- Received: from crystal2.unfcsd.unf.edu ([139.62.200.214]) 16, 28 -- by relay1.unf.edu with ESMTP; 18 Feb 2008 13:19:58 -0500 16, 28 -- Received: from emerald.unfcsd.unf.edu ([139.62.200.208]) by CRYSTAL2.unfcsd.unf.edu with Microsoft SMTPSVC(6.0.3790.1830); 16, 28 -- Mon, 18 Feb 2008 13:19:58 -0500 16, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 28 -- Content-class: urn:content-classes:message 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Type: text/plain; 16, 28 -- charset="iso-8859-1" 16, 28 -- Subject: FW: [Microscopy] W Filament and ESEM 16, 28 -- Date: Mon, 18 Feb 2008 13:19:57 -0500 16, 28 -- Message-ID: {D80383FB8CD3474B81CA3D67F248622901EBDCEA-at-EMERALD.unfcsd.unf.edu} 16, 28 -- X-MS-Has-Attach: 16, 28 -- X-MS-TNEF-Correlator: 16, 28 -- Thread-Topic: [Microscopy] W Filament and ESEM 16, 28 -- Thread-Index: Achwx/SxjDPS34sZS3+r3CYwpmVcVQBkdp+1 16, 28 -- References: {200802161815.m1GIFlXd000803-at-ns.microscopy.com} 16, 28 -- From: "Saredy, Jason" {sarj0007-at-unf.edu} 16, 28 -- To: {Microscopy-at-microscopy.com} 16, 28 -- X-OriginalArrivalTime: 18 Feb 2008 18:19:58.0243 (UTC) FILETIME=[DE4F6F30:01C8725A] 16, 28 -- Content-Transfer-Encoding: 8bit 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1IIJxhf002722 ==============================End of - Headers==============================
A possible option is to put the specimen in a SEM sample tube, drill a hole in the plastic bottom, put clear lid on and place the whole thing is a Sample Saver 100. Pump down the Sample Saver, close the Saver and then the pet cock. The sample will be under vacuum for quite awhile.
These units are pretty handy for either transporting specimens under vacuum or N2 purge.
No financial interest in SS-100...I just use them.
gary g.
At 09:44 AM 2/18/2008, you wrote:
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==============================Original Headers============================== 12, 21 -- From gary-at-gaugler.com Mon Feb 18 12:26:41 2008 12, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1IIQVDj011059 12, 21 -- for {microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 12:26:38 -0600 12, 21 -- Message-Id: {200802181826.m1IIQVDj011059-at-ns.microscopy.com} 12, 21 -- Received: (qmail 21099 invoked from network); 18 Feb 2008 09:58:29 -0800 12, 21 -- Received: by simscan 1.1.0 ppid: 21095, pid: 21097, t: 0.1001s 12, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 12, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 12, 21 -- by smtp2 with SMTP; 18 Feb 2008 09:58:29 -0800 12, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 21 -- Date: Mon, 18 Feb 2008 09:58:24 -0800 12, 21 -- To: drix00-at-gmail.com 12, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 12, 21 -- Subject: Re: [Microscopy] Looking for an in-situ FIB/TEM instrument in 12, 21 -- Canada or East of North America 12, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 12, 21 -- In-Reply-To: {200802181744.m1IHiu4D023710-at-ns.microscopy.com} 12, 21 -- References: {200802181744.m1IHiu4D023710-at-ns.microscopy.com} 12, 21 -- Mime-Version: 1.0 12, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-60D435D8 ==============================End of - Headers==============================
Thanks for the input. But I'm "stuck" with EBSD as the analytical method.
I forgot some important details about the whole stress scenario(s). The first scenario is under the JESD22 temperature, pressure and DC static bias with timed changes to bias. The second scenario is quite different.
This setup takes a COTS IC, delayers it and runs EBSD on some of the interconnects. Flavor two heats the IC (these are small pieces of the original larger die) to 150C in the SEM for up to 150 hours. Every twenty hours, each selected interconnect is EBSD scanned--heating stays on. At the end of the run the last EBSD scan is done.
Flavor three has the IC specimen planar in the SEM. Selected interconnects are given mouse bites with a FIB and then put in the SEM at 150C and current is run through each one at a time using dual Omniprobe Autoprobe 200 computer controlled probes and a constant current source (about 2A/cm^2). The before and after metal failure EBSD data is compared.
The third flavor is a similar current and temperature stress of a fabricated serpentine Al pattern with and without a mouse bite. One set are on TEOS while another are on Ti wetting layer.
At Scanning 2005 in Monterey, I gave a presentation on flavor two. This showed a bath tub shaped curve of grain size over time. Recrystalization was occurring and the terminal grain size was less than the original size. But the preferred {111} orientation (Cu in this case) was very well maintained. Grain stress also was of no issue. Work on Al is different and very much depends on initial deposition of the Al. Grain boundary integrity can vary widely.
The main problem at this point is to define the bias levels and time points for the JESD22 stress testing. I am not finding any references for this.
gary g.
At 08:11 AM 2/18/2008, you wrote:
} At 02:52 PM 2/15/2008, gary-at-gaugler.com wrote: } Dear Gary } } This looks like an ideal application for STM.. and most likely STM + } Raman. You should able to easily measure voltage input vs } output. With Raman, you should be able to see the stress in the } grain change the Raman signal as well as to image it with the AFM. } } From the instrumentation side, there are several approaches. I } wrote an article about a year ago for American Lab in which I } discussed "integrated" vs. "interfaced" systems. Integrated are } complete, all in one systems. NTMDT (Russia) was the first to } built a fully integrated systems, with all functions of both the } spectrometer and AFM/STM integrated into one control } package. AIST-NT has now started to do something similar, I } believer. Marshall Bates can help you more on that (marshall-at-aist-nt.com. } } Interfaced systems marry AFMs to Raman systems, with each component } still under its own operating software. Horiba-JY and Renishaw are } the two main providers in this realm. Both work with a variety of } existing AFM/STM systems. } } Witec makes clever integrated systems, but I am not sure if they are } able to do the electrical measurements. However, they are worth looking into. } } Hope this was helpful. } } Caveat: MME has no commercial interest } Barbara Foster, President } } Microscopy/Microscopy Education } 7101 Royal Glen Trail, Suite A } McKinney TX 75070 } P: (972)924-5310 } Skype: fostermme } W: www.MicroscopyEducation.com } } } MME is now scheduling customized, on-site courses through July } 2008. Call us today for details. } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Greetings to all: } } } } I'm working on a IC reliability topic and } } am wondering if anyone on the list has inputs } } about some of the issues. } } } } The work is to stress IC using HALT/HAST but } } per JESD22-A110-B. The problem is to identify } } the amount of bias change per step and at what } } time points to change the bias. My feeling is } } that some amount of time is at normal bias, then } } some time later at normal+10%, then +20% and } } then 30%. After that, the cycle starts over. } } } } When all of this is done, the EBSD grain data } } of interconnect metal will be compared to } } un-stressed grain data. The ICs could be flip } } chip BGA in plastic packages and/or ceramic. } } The die are removed from the package and then } } de-layered using plasma. Interconnects are } } Copper damascene and also Aluminum damascene } } and non-damascene. } } } } Any one have any ideas about this? Off-list is } } fine. All inputs are appreciated. } } } } gary g. } } } } } } ==============================Original Headers============================== } } 7, 17 -- From gary-at-gaugler.com Fri Feb 15 14:39:50 2008 } } 7, 17 -- Received: from smtp1.mc.surewest.net } (qsmtp.mc.surewest.net [66.60.130.145]) } } 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id m1FKdobI009118 } } 7, 17 -- for {microscopy-at-microscopy.com} ; Fri, 15 Feb 2008 } 14:39:50 -0600 } } 7, 17 -- Message-Id: {200802152039.m1FKdobI009118-at-ns.microscopy.com} } } 7, 17 -- Received: (qmail 13865 invoked from network); 15 Feb 2008 } 12:39:49 -0800 } } 7, 17 -- Received: by simscan 1.1.0 ppid: 13862, pid: 13863, t: 0.1901s } } 7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 } } 7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) } } 7, 17 -- by smtp1 with SMTP; 15 Feb 2008 12:39:49 -0800 } } 7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } } 7, 17 -- Date: Fri, 15 Feb 2008 12:39:47 -0800 } } 7, 17 -- To: MSA listserver {microscopy-at-microscopy.com} } } 7, 17 -- From: Gary Gaugler {gary-at-gaugler.com} } } 7, 17 -- Subject: IC stress testing prior to EBSD } } 7, 17 -- Mime-Version: 1.0 } } 7, 17 -- Content-Type: text/plain; charset=us-ascii; } format=flowed; x-avg-checked=avg-ok-2C8A5BC5 } } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 21 -- From gary-at-gaugler.com Mon Feb 18 12:44:51 2008 13, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1IIiosM028955 13, 21 -- for {microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 12:44:50 -0600 13, 21 -- Message-Id: {200802181844.m1IIiosM028955-at-ns.microscopy.com} 13, 21 -- Received: (qmail 15308 invoked from network); 18 Feb 2008 09:44:49 -0800 13, 21 -- Received: by simscan 1.1.0 ppid: 15305, pid: 15306, t: 0.1696s 13, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 13, 21 -- by smtp2 with SMTP; 18 Feb 2008 09:44:49 -0800 13, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 21 -- Date: Mon, 18 Feb 2008 09:44:44 -0800 13, 21 -- To: Barbara Foster {bfoster-at-mme1.com} 13, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 21 -- Subject: Re: [Microscopy] IC stress testing prior to EBSD 13, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 21 -- In-Reply-To: {20080218161409.5421D2E443-at-mx2.calweb.com} 13, 21 -- References: {200802152050.m1FKo7ss021138-at-ns.microscopy.com} 13, 21 -- {20080218161409.5421D2E443-at-mx2.calweb.com} 13, 21 -- Mime-Version: 1.0 13, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-60D435D8 ==============================End of - Headers==============================
To all those who would llike to participate in Microscopy and Microanalysis 2008 in Albuquerque:
Due to the Monday holiday, you now have until 5 PM Pacific Standard Time on Tuesday to submit your paper.
Please go to http://bono.cup.org to submit your paper.
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See http://mm2008.microscopy.org for meeting details
Best regards,
Mike Marko for the M&M2008 Program Committee
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==============================Original Headers============================== 14, 29 -- From marko-at-wadsworth.org Mon Feb 18 14:36:25 2008 14, 29 -- Received: from mailserv.wadsworth.org (mailserv.wadsworth.org [199.184.30.43]) 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1IKaPxP012869 14, 29 -- for {microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 14:36:25 -0600 14, 29 -- Received: from info.wadsworth.org (nnewton [199.184.30.36]) 14, 29 -- by mailserv.wadsworth.org (8.13.8+Sun/8.13.8) with ESMTP id m1IKa03Q012452 14, 29 -- for {microscopy-at-microscopy.com} ; Mon, 18 Feb 2008 15:36:00 -0500 (EST) 14, 29 -- Received: from 199.184.30.23 14, 29 -- (SquirrelMail authenticated user marko) 14, 29 -- by info.wadsworth.org with HTTP; 14, 29 -- Mon, 18 Feb 2008 15:36:20 -0500 (EST) 14, 29 -- Message-ID: {57012.199.184.30.23.1203366980.squirrel-at-info.wadsworth.org} 14, 29 -- Date: Mon, 18 Feb 2008 15:36:20 -0500 (EST) 14, 29 -- Subject: MM2008 deadline now Tuesday 14, 29 -- From: "Michael Marko" {marko-at-wadsworth.org} 14, 29 -- To: microscopy-at-microscopy.com 14, 29 -- Reply-To: marko-at-wadsworth.org 14, 29 -- User-Agent: SquirrelMail/1.4.9a 14, 29 -- MIME-Version: 1.0 14, 29 -- Content-Type: text/plain;charset=iso-8859-1 14, 29 -- Content-Transfer-Encoding: 8bit 14, 29 -- X-Priority: 3 (Normal) 14, 29 -- Importance: Normal 14, 29 -- X-Wadsworth-MailScanner-Information: Please contact CSS for more information 14, 29 -- X-Wadsworth-MailScanner: Found to be clean 14, 29 -- X-Wadsworth-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 14, 29 -- score=-100.002, required 5, autolearn=not spam, SPF_HELO_PASS -0.00, 14, 29 -- SPF_PASS -0.00, USER_IN_SPF_WHITELIST -100.00) 14, 29 -- X-MailScanner-From: marko-at-wadsworth.org ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both smithj-at-winthrop.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: smithj-at-winthrop.edu Name: Julian Smith III
Organization: Winthrop University, Rock Hill, SC
Title-Subject: [Filtered] [TEM] High-Pressure Freezer near Charlotte, NC, USA?
Question: TEM folk I'm looking for a lab with a high-pressure nitrogen freezer within driving distance--we're just south of Charlotte NC in the southeastern US. We have some small worms that resist traditional anesthesia/immersion fixation, and I'd like to try high-pressure freezing/freeze-sub. Contact me off-list, or call my cell 'phone. Thanks in advance, Julian -- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
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Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] Detergents for Pre-embedding Immunogold
Question: We have been starting to run pre-embedding immunogold experiments. For monolayers of osteoblasts, we have used 0.05% Triton-X-100 as a detergent for permeabilization with mixed results. I was wondering what detergents and concentrations others have employed successfully. Also, I was wondering if there are any detergents that can be used for the detection of membrane-bound antigens. I have heard that saponin is a less harsh detergent and that it must be used in every step of the procedure, but I do not know what concentration is recommended.
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Question: For those still hoping to submit a paper to M&M2008, don't panic!
Due to President's day in the US, some labs may be closed today, so we are extending the paper submission deadline until Tuesday at 5 PM Pacific Standard Time.
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Question: Hello, Is anybody aware of how to incorporate drift correction in Cathodoluminescence measurement? The measurement is done in an SEM chamber with 15kV, 30-90Kx mag, ~10-12 min acquisition time. thanks -pratik
We use 0.1% Saponin. It does not destroy the ultrastructural appearance of the membranes, however the antibodies do not penetrate very deep into the tissue so I can not say whether it is very effective.
good luck
Gerd
-- Dr. Gerd Leitinger
Laboratory Coordinator Ultrastructure Research Center for Medical Research Medical University of Graz
Postal address: Institute of Cell Biology, Histology and Embryology Harrachgasse 21 8010 Graz Austria Tel. +43 316 380 4237 Fax. +43 316 380 9625 Mailto: Gerd.Leitinger-at-meduni-graz.at
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Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] Detergents for Pre-embedding Immunogold
Question: We have been starting to run pre-embedding immunogold experiments. For monolayers of osteoblasts, we have used 0.05% Triton-X-100 as a detergent for permeabilization with mixed results. I was wondering what detergents and concentrations others have employed successfully. Also, I was wondering if there are any detergents that can be used for the detection of membrane-bound antigens. I have heard that saponin is a less harsh detergent and that it must be used in every step of the procedure, but I do not know what concentration is recommended.
Invitrogen sells a line of "Click-iT" reagents that are metabolically incorporated into glycoproteins and then post-labeled with (for example) biotin. The chemistry uses an azide modified sugar which is metabolically incorporated and then reacted in the presence of copper with an alkyne linked to a reporter molecule. They are marketed as a method to detect glycoproteins after western blots.
I was wondering if anyone had tried imaging these reagents in cell culture?
I have NO connection with Invitrogen except for being an occasional customer.
Thanks, Mike
Michael J. Herron, U of MN, Dept. of Entomology herro001-at-umn.edu 612-624-3688 (office) 612-625-5299 (FAX)
==============================Original Headers============================== 9, 17 -- From herro001-at-umn.edu Tue Feb 19 12:32:17 2008 9, 17 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1JIWH0v009235 9, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Feb 2008 12:32:17 -0600 9, 17 -- Received: from [134.84.48.158] (x84-48-158.ej2393.umn.edu [134.84.48.158]) 9, 17 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 9, 17 -- Tue, 19 Feb 2008 12:32:17 -0600 (CST) 9, 17 -- X-Umn-Remote-Mta: [N] x84-48-158.ej2393.umn.edu [134.84.48.158] #+LO+TS+AU+HN 9, 17 -- Mime-Version: 1.0 (Apple Message framework v753) 9, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 17 -- Message-Id: {77FAB705-123A-4A85-A579-26653C1FFC87-at-umn.edu} 9, 17 -- Content-Transfer-Encoding: 7bit 9, 17 -- From: Michael Herron {herro001-at-umn.edu} 9, 17 -- Subject: Click-iT imaging? 9, 17 -- Date: Tue, 19 Feb 2008 12:32:18 -0600 9, 17 -- To: Microscopy-at-microscopy.com 9, 17 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
Good Afternoon: We have the Leica HPF. I will be freezing cell suspensions using the copper tube assembly to go into the cryomicrotome. It would appear that in order to get vitreous preps one should use a cryoprotectant. My question to the list is what cryoprotectants have proven useful and do they vary in composition with cell type? Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Tue Feb 19 12:57:52 2008 5, 26 -- Received: from gigi.cs.uoguelph.ca (gigi.cs.uoguelph.ca [131.104.94.210]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1JIvqO6022726 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 19 Feb 2008 12:57:52 -0600 5, 26 -- Received: from gimli.cs.uoguelph.ca (gimli.cs.uoguelph.ca [131.104.94.49]) 5, 26 -- by gigi.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m1JIvoIk022097 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 19 Feb 2008 13:57:50 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by gimli.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m1JIvoA1015733 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 19 Feb 2008 13:57:50 -0500 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Tue, 19 Feb 2008 13:57:50 -0500 5, 26 -- Message-ID: {20080219135750.9jfiqqok0cwcw4s4-at-webmail.uoguelph.ca} 5, 26 -- Date: Tue, 19 Feb 2008 13:57:50 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: High Pressure Freezing Cryoprotectant 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.210 ==============================End of - Headers==============================
Given that some of your samples contaminate and some do not, I would look first to cleanliness in sample preparation. It is easy to contaminate a sample with dirty solvents, such as acetone and methanol. Crystal bond (mounting wax) can also easily contaminate a sample. After soaking, it must be rinsed off thoroughly with clean acetone (or methanol) then blown dry.
Plasma cleaning is a good solution for most samples. If you use a plasma cleaner, it is best to clean prior to any TEM examination. An area exposed to the electron beam will be much harder to clean.
You can also observe the sample warm (100-200C) or cold (LN temperature) if the sample is compatible with either. Sample geometry can be significant, since the contamination is often migrating across the surface and polymerizing under the beam. In this case a flat sample will be worse.
Using the cold trap in the TEM will help if the contamination is coming from the TEM.
In general, I would tend to trust the carbon data if it does not change on the timescale of data collection.
I have not observed any correlation between the time spent in a PIPS and level of contamination. I find the argument from South Bay Technology (see below) that a PIPS can contaminate another instrument that shares an Ar supply to be not credible. The vapor pressure of the vacuum grease is 11 orders of magnitude lower than the pressure of the Argon in the lift mechanism, and it is flushed out each time the stage is raised. Perhaps they had a customer who used vacuum grease that was not approved, used a supply line that was previously contaminated, or used a cylinder that contained residual compressor oil. To suggest that every PIPS will contaminate the Ar supply is inappropriate to this forum. Gatan uses and recommends perfluoropolyether grease (hydrocarbon free) for lubrication.
Good luck, Steve
Disclaimer: Gatan manufactures TEM sample preparation equipment, including the PIPS ion mill and the Solarus plasma cleaner. ========================================= Steven T. Coyle, Ph.D. R&D Manager, Specimen Preparation Equipment Gatan Inc. 5794 W. Las Positas Blvd. Pleasanton, CA 94588 Direct (925) 224 7383 Fax (925) 463 0204 E-mail scoyle-at-gatan.com =========================================
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Tuesday, February 12, 2008 5:55 PM To: scoyle-at-gatan.com
Let me hazard a guess or two.
I suspect that may be breaking down your carbide to form your amorphous carbon when you are focusing the beam onto the sample.
I had a sample of a pulsed laser deposited diamond-like carbon film. You can see an image of this film on our website in the application note #59 (http://www.southbaytech.com/appnotes/59%20EELS%20of%20PLD%20DLC.pdf) that was prepared by MicroCleaving(TM). The film was amorphous, but there are alternating light and dark bands in the DLC film. EELS showed that the darker bands were SP3 bonding while the light bands were SP2/SP3 or more graphitic. We only had a parallel EELS on a CM200FEG. When the spot was focused down in order to isolate a dark band, the earliest EELS spectra showed no Pi-star peak, but almost instantly, started growing one and a spot formed. If the beam was spread out, no contamination was seen. It was only in the highly focused beam that we got it and in the first instant, you could clearly see that the dark bands had no SP2 bonding. I always wanted to revisit these samples with a GIF to examine the sample without the conversion, similar to what Jim Bentley did with his GIF and carbon films.
Another question for you if you think that it is actual contamination is have you plasma cleaned your sample? If you always plasma clean your samples before putting them in the microscope and other samples do not contaminate in your microscope, then the source is not your sample and not your microscope, but is probably the mechanism that I described above.
For a source of contamination, check the O-rings and lubrication on your whisper lock on the PIPS. The same Ar gas that is used for the guns is used for the lift assembly. A colleague of mine has demonstrated conclusively that the Ar gas was contaminated by the lubrication from the lift mechanism in the PIPS. We strongly advise our customers who have either a Gentle Mill(TM) or an IV3/4 equipped with a low energy gun to not share the gas line with a PIPS instrument for that reason. Your difference in contamination could be the amount of time the samples were left in the PIPS. However, if you plasma clean your samples, you will eliminate this source or any other external source of contamination on your samples.
Disclaimer: South Bay Technology manufactures and sells the MicroCleave(TM) Kit and the PC-2000 Plasma Cleaner. We also distribute the Technoorg-Linda Gentle Mill(TM) and IV3/4 ion mills in the United States.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: z.zhou-at-sheffield.ac.uk [mailto:z.zhou-at-sheffield.ac.uk] Sent: Tuesday, February 12, 2008 3:55 PM To: Walck-at-SouthBayTech.com
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Email: z.zhou-at-sheffield.ac.uk Name: Zoe
Title-Subject: [Filtered] carbon contamination while doing EELS
Question: I found bad carbon contamination while put Cr3C2 samples in a 2010 FEGTEM for EELS analysis. They were thin film cross sections made by conventional sandwich method by Epoxy glue, and Gatan PIPS ion milling. I have examined three samples made by the same route, two turned out contaminated easily, but one seemed not much affected. The samples were subjected to selected area EELS in diffraction mode and STEM ADF imaiging. Carbon accumulated at the edge of the selected area circle. Once the beam was focused, carbon K-edge was enchanced in the EELS acquired. Amorphous carbon in EEL spectra stops me from studying the real carbon bonding from the Cr3C2 phase.
My questions are: (1)Why were some samples contaminated, other not much? (2)What could be the most possible contamination sources? (3)How can I minimise the contamination both during sample preperation and TEM examination? (3)How reliable is the carbon data acquired?
Shannon, I have seen some excellent ultrastructure in preparations utilizing ethanol for permeabilization of tissue. Usually 50% ethanol was used for one possibly two hours. However, the percentage of glutaraldehyde in the primary fixation is a significant factor. Good results can be obtained with as little as 0.1% glutaraldehyde and if you are lucky 0.05%. For a monolayer of cells I would suggest 15 to 30 minutes of 50% alcohol. There are many protocols described in scientific literature and so many techniques and variations you could spend the rest of your life trying them!
Here is one exemplary reference with very nicely detailed protocols: Llewellyn-Smith IJ, Dicarlo SE, Collins HL, Keast JR. Enkephalin-immunoreactive interneurons extensively innervate sympathetic preganglionic neurons regulating the pelvic viscera. J Comp Neurol. 2005 Aug 1;488(3):278-89.
Good luck, Larry
modla-at-dbi.udel.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: modla-at-dbi.udel.edu } Name: Shannon Modla } } Organization: University of Delaware } } Title-Subject: [Filtered] Detergents for Pre-embedding Immunogold } } Question: We have been starting to run pre-embedding immunogold } experiments. For monolayers of osteoblasts, we have used 0.05% } Triton-X-100 as a detergent for permeabilization with mixed results. } I was wondering what detergents and concentrations others have } employed successfully. Also, I was wondering if there are any } detergents that can be used for the detection of membrane-bound } antigens. I have heard that saponin is a less harsh detergent and } that it must be used in every step of the procedure, but I do not } know what concentration is recommended. } } Shannon } } } Login Host: 71.242.52.182 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Feb 19 08:32:54 2008 } 8, 11 -- Received: from [192.168.1.9] (msdvpn8.msd.anl.gov [130.202.238.72]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1JEWpkR002987 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 19 Feb 2008 08:32:53 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240806c3e09906cf32-at-[192.168.1.9]} } 8, 11 -- Date: Tue, 19 Feb 2008 23:32:51 +0900 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: modla-at-dbi.udel.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Detergents for Pre-embedding Immunogold } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 7, 29 -- From Larry.Ackerman-at-ucsf.edu Tue Feb 19 14:28:34 2008 7, 29 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1JKSWqM018996 7, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Feb 2008 14:28:33 -0600 7, 29 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 7, 29 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 7, 29 -- Tue, 19 Feb 2008 12:26:22 -0800 7, 29 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 7, 29 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 7, 29 -- with Microsoft SMTPSVC(6.0.3790.3959); Tue, 19 Feb 2008 12:28:23 -0800 7, 29 -- Message-ID: {47BB3BD6.6090309-at-ucsf.edu} 7, 29 -- Date: Tue, 19 Feb 2008 12:28:06 -0800 7, 29 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 7, 29 -- Reply-to: larry.ackerman-at-ucsf.edu 7, 29 -- Organization: UCSF, NeuroAnatomy 7, 29 -- User-Agent: Thunderbird 2.0.0.9 (Macintosh/20071031) 7, 29 -- MIME-Version: 1.0 7, 29 -- To: modla-at-dbi.udel.edu, Microscopy-at-microscopy.com 7, 29 -- Subject: Re: [Microscopy] viaWWW: Detergents for Pre-embedding 7, 29 -- Immunogold 7, 29 -- References: {200802191451.m1JEpwrX012661-at-ns.microscopy.com} 7, 29 -- In-Reply-To: {200802191451.m1JEpwrX012661-at-ns.microscopy.com} 7, 29 -- X-OriginalArrivalTime: 19 Feb 2008 20:28:23.0642 (UTC) 7, 29 -- FILETIME=[F97FC3A0:01C87335] 7, 29 -- X-WSS-ID: 6BA5E4E429O7120467-01-01 7, 29 -- Content-Type: text/plain; 7, 29 -- charset=iso-8859-1; 7, 29 -- format=flowed 7, 29 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jayma.moore-at-ndsu.edu Name: Jayma Moore
Organization: Electron Microscopy Center, North Dakota State University
Title-Subject: [Filtered] Cryosectioning woes
Question: We are attempting to add cryoultramicrotomy to our repertoire of services for materials research, and have installed an RMC Powertome XL with CRX cryo unit, but the extension from ambient-temperature ultramicrotomy hasn't been easy. I know many of you are thinking "Welcome to my world!" Does anyone know of a comprehensive course to help us get up to speed, in addition to the May offering by RMC in Tucson? Or can you recommend some references? Thanks.
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Email: mpokroy-at-cvpath.org Name: Marsha A Pokroy
Organization: CVPath Institute, Inc.
Title-Subject: [Filtered] Open TEM Position
Question: Cardiovascular research organization based in Gaithersburg, Maryland seeks a full-time Transmission Electron Microscopy (TEM) Specialist. The specialist would assist with the analysis of cardiovascular research material using new TEM equipment. Candidate must have hands on experience working with TEM, and at least a college level science/histology background. Send resume/CV with slary requirements to Marsha Pokroy at mpokroy-at-cvpath.org.
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Email: phass-at-ethz.ch Name: Philipp Hass
Organization: ETH Z¸rich
Title-Subject: [Filtered] Staining of wood
Question: Hello, We are doing some investigations on glue-lines in wood. Our standard procedure so far is to boil the wood in water to soften it, produce microtome slices and stain them. However the boiling process might affect the condition of the adhesive and the wood itself. To avoid this effect, we want to survey little wooden cubes (untreated), where the investigated surface has only been smoothed with the microtome. To get a better contrast between the wood and the adhesive, we tried various staining methods (safranine, iodide-iodide-potassium, toluidine-blue), that have been mentioned in the literature. Anyway, the tests results were not satisfying. Not every part of the adhesive was dyed by the staining agent to the same degree making a determination of the adhesive penetration very difficult. My question now is: Has anybody some experience with a dry sample preparation of wood or glue lines respectively? And has anybody some experience with the staining of wood or adhesive? The wood is beech; the adhesives are PUR, UF and PVAC. Thanks, Philipp
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Email: 2kjc2-at-queensu.ca Name: Krys
Organization: Queen's University
Title-Subject: [Filtered] Sliding microtome?
Question: I was wondering if anyone has, or knows someone who has, a sliding microtome. I doing some tree-ring analysis on small Arctic willow stems, and the typical sanding procedures are too coarse for the microscopic rings. I'm following a methodology that calls for 15 micron sections cut on a sliding microtome. From what I've heard, a rotary microtome does not have the strength to cut this harder material. Since this is likely a one-time experiment, I don't want to buy one, I'm just looking for someone who may have one they're willing to cut my samples on. If anyone can help me out, can you please let me know? Thank you.
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Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] Detergents for Pre-embedding Immunogold and More
Question: I would like to thank those who have responded to my inquiry. Currently, the suggestions were:
1. 0.5% Triton-X-100 for 5 min in PBS For saponin, use 0.1% in PBS As a reference, refer to Humbel BM, De Jong, MDM, Muller, WH, and Verkleij AJ. 1998. Pr embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc Res Tech 42: 43-58.
2. 0.1% saponinóproduces improved ultrastructural preservation but poor antibody penetration
3. NEW Triton-X-100 at 0.01% during the block only. Incubate in primary overnight. For saponin, use 0.05-0.1% in every step.
4. 0.05% Saponin in every step. Saponin basically sits between (intercalates) cholesterol groups on the plasma membrane, once removed those "holes" seal up. The deeper you get into a cell (less cholesterol groups) the less permeabilization you get and hence labelling.
As a preface to my original question, we are using ultrasmall gold conjugates from Aurion and the Aurion SE-EM silver enhancement kit. We were attempting to perform a double-labeling experiment by using different enhancement times for the different primary antibodies. Both primaries are raised in the same species so labeling had to be performed consecutively. After the final enhancement, the samples were briefly postfixed with 0.5% OsO4 for 15 min. We used the following paper as an outline: Yi H, Leunissen JLM, Shi GM, Gutekunst CA and Hersch SM. 2001. A novel procedure for pre-embedding double immunogold-silver labeling at the ultrastructural level. J Histochem and Cytochem. 49: 279-284. The initial results showed a variation in the size of the particles such that the variation was more of a continuum rather than two discretely sized populations. There are numerous explanations for the negative results such as poor antibody penetration, a problem with the silver enhancement, or experimental error. To test the silver enhancement reaction, I adsorbed secondary antibody on poly-l-lysine coated grids and performed the enhancement. The outcome also showed a wide variation in the size of enhanced particles, leading me to wonder if this was normal. Although statistics were not performed, certain sized particles did seem more numerous than others, but the variation was still surprising to me. Therefore, this brings me ask more questions: Is there a way to make the silver enhancement reaction more uniform? Is the variability in the enhancement a result of a variation in the size of the ultrasmall gold conjugates or a variation in the enhancement reaction itself? Are there other silver enhancers/ gold enhancers that produce a more uniform reaction product? Has anyone else had success performing double-labeling using ultrasmall gold and silver enhancement and if so are there any tips or tricks?
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Email: kwylly-at-ppg.com Name: Kelly Wylly
Organization: PPG Industries, Inc.
Title-Subject: [Filtered] Quantomix WETSEM
Question: Has anyone had any experience (positive or negative) with the Quantomix WETSEM capsules? I found some comments by searching the archives, but I would like to correspond with anyone that has prepared a sample an placed it in their SEM.
In addition, there was a suggestion in past comments regarding SPI's "Wet Cell" for similar applications. Has anyone tried this option?
I would strongly consider RMC's in-depth workshop. No vendor's half-day of training will get you where you need to be. Leica also offers similar workshops. Although the Leica and RMC tools are different, the concerns are identical. In the end, you'll be better off if you can attend a good RMC workshop. You might also check to see if McCrone offers a workshop on cryomicrotomy of materials.
If you are cutting polymers, there are a few key points to keep in mind. (1) Know the glass transition temperature (Tg) of the polymer. Keep the knife and specimen temperatures well below Tg. If you aren't sure about Tg, look it up. Sawyer and Grubb's "Polymer Microscopy" will probably help. (2) Always keep the block faces small. (3) Cut slowly (0.1 - 0.3 mm/sec). (4) When not cutting, keep the specimen very close to the level of the knife edge to maintain the temperature of the specimen face about the same temperature as the knife edge. This helps prevent those infuriating moments when you cut only a very thick section or none at all. (5) Increase the speed (shorten the time) of the return stroke on the microtome to minimize the amount of time that the specimen spends above the knife edge (warmer) and below the knife edge (colder).
The hard part of cryo-ultramicrotomy is not cutting the sections but collecting them. Using the grid holder supplied with the microtome, position the grid very close to the knife edge. Carefully manipulate the section from the knife edge onto the grid using a herding hair like you would use for ambient temperature ultramicrotomy.
All this is easily said but difficult to do in practice. Practice makes perfect (or at least acceptable).
Best of luck,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
jayma.moore-at-nd su.edu To gary.m.brown-at-exxonmobil.com 02/19/08 04:59 cc PM Subject [Microscopy] viaWWW: Cryosectioning Please respond woes to jayma.moore-at-nd su.edu
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Title-Subject: [Filtered] Cryosectioning woes
Question: We are attempting to add cryoultramicrotomy to our repertoire of services for materials research, and have installed an RMC Powertome XL with CRX cryo unit, but the extension from ambient-temperature ultramicrotomy hasn't been easy. I know many of you are thinking "Welcome to my world!" Does anyone know of a comprehensive course to help us get up to speed, in addition to the May offering by RMC in Tucson? Or can you recommend some references? Thanks.
The STEM (Scanning Transmission Electron Microscope) at BNL (Brookhaven National Laboratory) is a User Facility.
It is a custom-built electron microscope optimized for imaging unstained biological molecules with minimal radiation damage.
Freeze-dried, unstained, unshadowed, isolated biological molecules are imaged in-focus, which permits:
1. Mass measurements to: - Determine stoichiometry and homogeneity of a complex. - Compare modified or re-assembled complexes with native structures. - Determine filament symmetry from mass per unit length.
2. Optimal visualization of metal clusters used as specific heavy atom labels.
3. Simultaneous imaging of DNA, protein, and gold labels in a complex.
Details about appropriate specimens, starting a new project, fee-for-service, microscope design and operation, and projects and publications, can be found on our web page at: http://www.biology.bnl.gov/stem/stem.html
Currently, the STEM facility has partial support from DOE and partial support from fee-for-service projects.
Inquiries should be directed to: Joseph Wall, P.I. wall-at-bnl.gov Martha Simon msimon-at-bnl.gov
==============================Original Headers============================== 10, 23 -- From msimon-at-bnl.gov Wed Feb 20 10:03:36 2008 10, 23 -- Received: from smtpgw.bnl.gov (smtpgw.bnl.gov [130.199.3.132]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1KG3aZB010914 10, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 20 Feb 2008 10:03:36 -0600 10, 23 -- Received: from orca.bio.bnl.gov ([130.199.120.74]) 10, 23 -- by smtpgw.bnl.gov with esmtp (bnl.gov SMTP on gw4) 10, 23 -- serial 1JRrPv-0007NN-DB; Wed, 20 Feb 2008 11:03:31 -0500 10, 23 -- Message-ID: {47BC4F53.5020704-at-bnl.gov} 10, 23 -- Date: Wed, 20 Feb 2008 11:03:31 -0500 10, 23 -- From: Martha Simon {msimon-at-bnl.gov} 10, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 10, 23 -- X-Accept-Language: en-us, en 10, 23 -- MIME-Version: 1.0 10, 23 -- To: Microscopy-at-Microscopy.Com 10, 23 -- CC: Simon MN {msimon-at-bnl.gov} , Wall {wall-at-bnl.gov} 10, 23 -- Subject: STEM at BNL: A User Facility 10, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 23 -- Content-Transfer-Encoding: 7bit 10, 23 -- X-BNL-MailScanner-Information: Please contact the ITD Service Desk for more information 10, 23 -- X-BNL-MailScanner: Found to be clean 10, 23 -- X-BNL-MailScanner-SpamCheck: 10, 23 -- X-BNL-MailScanner-From: msimon-at-bnl.gov 10, 23 -- X-Spam-Status: No ==============================End of - Headers==============================
We're thinking of changing our undergraduate Light Microscopy course text, and I thought I'd check with the microscopy gang. What we're looking for is a text that covers both the construction, optics (with theory, but not quantum optics or the like) and use of the light microscope, with DIC, Hoffman, fluorescence, etc. *and* microtechnique, preferably animal. Fixation, dehydration, embedding, sectioning, etc. I've got excellent examples of books that cover either the microscope *or* microtechnique, but not both. And, those are expensive. We're also hoping find something reasonably priced, not $80 to over $100, as most texts seem to be these days. (If necessary, we'll go with 2 books, one on the microscope and optics, and one on microtechnique, but again, price counts. 2 book, each one costing $60 or more is right out.) Any ideas? Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 20 -- From oshel1pe-at-cmich.edu Wed Feb 20 14:38:11 2008 3, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1KKcB8t006901 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Feb 2008 14:38:11 -0600 3, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m1KKrf9N028656 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Feb 2008 15:53:41 -0500 3, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 3, 20 -- Wed, 20 Feb 2008 15:37:53 -0500 3, 20 -- Mime-Version: 1.0 3, 20 -- Message-Id: {f0624080ec3e23fd80d6c-at-[141.209.160.249]} 3, 20 -- Date: Wed, 20 Feb 2008 15:38:07 -0500 3, 20 -- To: Microscopy-at-microscopy.com 3, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 20 -- Subject: text recommendation biological microscopy 3, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 20 -- X-OriginalArrivalTime: 20 Feb 2008 20:37:53.0622 (UTC) FILETIME=[77A58F60:01C87400] 3, 20 -- X-CanItPRO-Stream: default 3, 20 -- X-Spam-Score: -4 () L_EXCH_MF 3, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
It seems to me that EMS could include this kind of information with the kit!
Stephane
----- Original Message ---- X-from: "dac-at-research.umass.edu" {dac-at-research.umass.edu} To: nizets2-at-yahoo.com Sent: Friday, February 15, 2008 2:43:05 AM
Thank you to all who sent information about the "new" Spurr's resin.
Since some replies came off-list, the answer is that this topic has been covered while I was happily working through my old stocks of Spurr's and not paying attention. In summary, there is information in the archives of the list and the formula does need adjusting from the old proportions. The key work on this seems to be the Ellis papers:
Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations"
Corrected Formulation for Spurr Low Viscosity Embedding Medium Using the Replacement Epoxide ERL 4221. Microsc Microanal 12(Supp 2), 2006 E. Ann Ellis Microscopy and Imaging Center, BSBW 119/MS 2257, Texas A&M University, College Station, TX 77843-2257
These instructions also give a new formulation: http://www.2spi.com/catalog/chem/instructions_spurr_kits.html
Again, thank you to all who replied.
Dale Callaham
==============================Original Headers============================== 8, 21 -- From dac-at-research.umass.edu Thu Feb 14 19:37:38 2008 8, 21 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1F1bcnR010703 8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 19:37:38 -0600 8, 21 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 8, 21 -- (authenticated bits=0) 8, 21 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1F1bbvh010309 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Thu, 14 Feb 2008 20:37:38 -0500 8, 21 -- Message-ID: {47B4ECED.7050500-at-research.umass.edu} 8, 21 -- Date: Thu, 14 Feb 2008 20:37:49 -0500 8, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Microscopy List {microscopy-at-microscopy.com} 8, 21 -- Subject: Re: [Microscopy] Spurr's Resin - new formula -question ANSWERED, 8, 21 -- THANKS 8, 21 -- References: {3.0.6.32.20080214154534.007bbe20-at-pop3.norton.antivirus} 8, 21 -- In-Reply-To: {3.0.6.32.20080214154534.007bbe20-at-pop3.norton.antivirus} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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==============================Original Headers============================== 20, 19 -- From nizets2-at-yahoo.com Thu Feb 21 04:42:36 2008 20, 19 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 20, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1LAgaYI030837 20, 19 -- for {microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 04:42:36 -0600 20, 19 -- Received: (qmail 13393 invoked by uid 60001); 21 Feb 2008 10:42:35 -0000 20, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 19 -- s=s1024; d=yahoo.com; 20, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 20, 19 -- b=Q1MmHN4NGRju5mSMumSPS49yLBc6W8pfzsKh46rIgK5KzzZ6e5Mus9yEkTcrfpPM608IRL95qEEhiVZZy6r3HMD7+nD0KY6a3H0bgG+7obc1+DUAzS2lwPBJcgiZ6pMnvjntcr1MNpPw+9BMhuTRVviDJNU+/h3WZVnEDXH2hYg=; 20, 19 -- X-YMail-OSG: KR1pcPgVM1m4CzYWhZiAur2TO5r.3szu1JtQPh8y8bOzCXfpd42aYvphehKfBnNj8thyOj0uSroTewDAqOLwGSTYrJKbA05DpeXLFjeyy8ogoV9YlhbBpBFerCeDcC4kMBEbbphhdvPnRZdRMKjB1snW5dRE3CRKqMXqRD.Pow42a2T4ZDxkLgQ- 20, 19 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Thu, 21 Feb 2008 02:42:35 PST 20, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 20, 19 -- Date: Thu, 21 Feb 2008 02:42:35 -0800 (PST) 20, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 20, 19 -- Subject: Re: [Microscopy] Re: Spurr's Resin - new formula -question ANSWERED, 20, 19 -- To: microscopy-at-microscopy.com 20, 19 -- MIME-Version: 1.0 20, 19 -- Content-Type: text/plain; charset=us-ascii 20, 19 -- Message-ID: {402108.9720.qm-at-web37402.mail.mud.yahoo.com} ==============================End of - Headers==============================
Good morning to all. I have noticed a good deal of correspondence on the above subject and would like to offer some assistance if possible. Here at EMS we have been working with many labs over the past 2 years and have worked on many different types of samples to try to come up with the best formulations on using the new ERL 4221 in place of the 4206 in the Low Viscosity Kit. We have learned that in some cases the ERL 4221 can replace the 4206 without any formula change whatsoever; however we have also learned that there are distinct differences in the original ERL and the replacement ERL which causes many samples to be problematic. Below you will find modified formulations which seems to offer comparable results to the original Spurrs formulation. We have broken down our formulations to general tissue and samples and then we offer totally new recipes and procedures for more difficult samples. I hope that you find this work helpful and we look forward to answering any questions that you may have
Formulation: See end of Document for special formulations
ERL 4221 10 g DER 736 8 g NSA 25 g DMAE 0.3 g Cure Time at 70°C 8 hours Pot Life 3-4 days
Mixing Instructions: Add each component in turn to a disposable plastic beaker. An exact weight is recommended, and care must be used in dispensing the final amounts of each component so that no excess is added. The catalyst (DMAE) should be added last, after gently mixing the three other components. The complete formula should be mixed thoroughly. The complete mixture with the hardener can be used immediately for infiltration, and then for embedding. Although the mixture can be stored in a disposable syringe, well capped and with no air, in a freezer for several months it is highly recommended that freshly prepared embedding medium always be used. If you choose to store the mixture, it is imperative that you warm it thoroughly prior to use. Dehydration - Infiltration and Polymerization: This embedding media is compatible with all dehydrating agents: acetone, dioxane, ethanol, hexyleneglycol, isopropyl alcohol, propylene oxide, tert-butyl alcohol. The schedule and concentration can be established by the investigator however for a rule of thumb we recommend the following: Dehydration is generally done at room temperature in a graded series of Ethanol starting at 50% then going to 70, 95 and 100% for no less than 20-30 minutes each step. All dehydrating agent must totally be removed during infiltration due to the fact that it will effect curing. The embedding media is completely compatible with ethanol. Thus, it is not mandatory to have a change to propylene oxide prior to infiltration as is true for other epoxy resin mixtures. If working with plant cells it is recommended to use propylene oxide. The infiltration (one should employ a specimen rotator) can be started by adding the embedding media to the dehydrating fluid left in the vial with the tissue(1:2) . Swirl the mixture and allow it to stand for 2- 3 hours. Then replace with a 1:1 dehydrating agent/embedding medium, swirl and allow to sit overnight. Replace with a 1:3 dehydrating agent/embedding medium, swirl, and allow it to stand for another 2 to 3 hours. Pour and drain the mixture and add fresh embedding media (100% Spurr). For small specimens, 5-6hours; for large specimens, 5-6 hours followed by overnight. Curing takes 16-24 hours at 60°C. (The mixture can be left in an oven overnight).
Special Formulations:
For Biopsies from Kidney, Liver, GI, Esophagus Mixing Directions : ERL 4221 18ml DER 736 14ml NSA 48 ml DMAE 0.6ml Dehydration: 50%, 70%, 95% ETOH for 20-30 minutes each change 2 x 100% ETOH for 30 minutes Infiltration*: 1 part Spurr to 2 parts ETOH for 3 hours 1 TO 1 overnight/over weekend 3 to 1 (Spurr to ETOH) 3 hours 100% Spurr 6-8 hours Polymerization: Then Polymerize overnight (12 hour Max) at 70-80 Degrees C *All infiltration Procedures shall be run on a Rotator.
For Biopsies from Muscle, Nerve or Skin
Dehydration: 50%, 70%, 95% ETOH for 20-30 minutes each change 2 x 100% ETOH for 30 minutes Infiltration*: (Spurr/ETOH 100%) 1 part Spurr to 2 parts ETOH 24 hours (over weekend) 1 TO 1 24 hours (over weekend) 3 to 1 (Spurr to ETOH) 24 Hours 100% Spurr 24 Hours Polymerization: Then Polymerize overnight (12 hour Max) at 70-80 Degrees C *All infiltration Procedures shall be run on a Rotator.
Stacie Kirsch Electron Microscopy Sciences 1560 Industry Road Hatfield Pa 19440 Tel: 215-412-8400 Fax: 215-412-8450 WWW.emsdiasum.com
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==============================Original Headers============================== 11, 23 -- From SGKCCK-at-aol.com Thu Feb 21 05:37:11 2008 11, 23 -- Received: from imo-d23.mx.aol.com (imo-d23.mx.aol.com [205.188.139.137]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1LBbBhA013254 11, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 05:37:11 -0600 11, 23 -- Received: from SGKCCK-at-aol.com 11, 23 -- by imo-d23.mx.aol.com (mail_out_v38_r9.3.) id w.d47.214f5ed5 (37164) 11, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 06:36:58 -0500 (EST) 11, 23 -- Received: from webmail-mf11 (webmail-mf11.webmail.aol.com [64.12.88.224]) by cia-ma04.mx.aol.com (v121.4) with ESMTP id MAILCIAMA043-912c47bd625a29; Thu, 21 Feb 2008 06:36:58 -0500 11, 23 -- To: Microscopy-at-microscopy.com 11, 23 -- Subject: Low Viscosity NEw Formulation 11, 23 -- Date: Thu, 21 Feb 2008 06:36:58 -0500 11, 23 -- X-MB-Message-Source: WebUI 11, 23 -- X-AOL-IP: 151.197.12.151 11, 23 -- X-MB-Message-Type: User 11, 23 -- MIME-Version: 1.0 11, 23 -- From: sgkcck-at-aol.com 11, 23 -- Content-Type: text/plain; charset="utf-8"; format=flowed 11, 23 -- X-Mailer: AOL Webmail 34032-STANDARD 11, 23 -- Received: from 151.197.12.151 by webmail-mf11.sysops.aol.com (64.12.88.224) with HTTP (WebMailUI); Thu, 21 Feb 2008 06:36:58 -0500 11, 23 -- Message-Id: {8CA42953407DD8A-EF8-2D28-at-webmail-mf11.sysops.aol.com} 11, 23 -- X-Spam-Flag: NO 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1LBbBhA013254 ==============================End of - Headers==============================
First, thanks for the many responses. I'll be posting a book list. When I get more than a few. Mostly, people are recommending the various web sites, such as Molecular Expressions, Nikon's MicroscopyU, Olympus' site, and so forth. These are all excellent resources, and yes, have good Java tutorials. We do use these sites in our courses (and wish the EM companies would do the same for the EM and scanned-probe microscopies). But. We're looking for actual books. The kind the students can puzzle over, write in, keep for life (lots of the best light microscopy fixation and staining methods are in things like Bolles Lee, the Vade-Mecum, and so on ...) and cuddle in bed.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 20 -- From oshel1pe-at-cmich.edu Thu Feb 21 07:05:43 2008 3, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1LD5hiZ029142 3, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 07:05:43 -0600 3, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m1LDLC9N020715 3, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 08:21:12 -0500 3, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 3, 20 -- Thu, 21 Feb 2008 08:05:26 -0500 3, 20 -- Mime-Version: 1.0 3, 20 -- Message-Id: {f06240800c3e326a45666-at-[141.209.160.249]} 3, 20 -- Date: Thu, 21 Feb 2008 08:05:40 -0500 3, 20 -- To: Microscopy-at-microscopy.com 3, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 20 -- Subject: text recommendations update 3, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 20 -- X-OriginalArrivalTime: 21 Feb 2008 13:05:26.0700 (UTC) FILETIME=[6D3BBEC0:01C8748A] 3, 20 -- X-CanItPRO-Stream: default 3, 20 -- X-Spam-Score: -4 () L_EXCH_MF 3, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
J.D. Picket-Heaps = Jeremy Picket-Heaps School of Botany University of Melbourne
He visited the states last July and did a one-day course on "Working with Living Cells - Triumphs, Tribulations and Tragedies"
Very helpful, very nice. If you get an email to him (I don't have one handy) he would likely respond directly to you.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
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-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Thursday, February 21, 2008 9:40 AM To: ph2-at-sprynet.com
Search for papers by J.D. Pickett-Heaps. I don't have a reference, but I know that he has done classic work on algae and diatoms.
Dale
F.Christie-at-rbge.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Everyone, } } I'd be grateful if someone could recommend a protocol for diatom } embedding for TEM sectioning as this material is new to me. } } Thanks, } } Frieda } } } Frieda Christie, } Electron Microscopist, } Royal Botanic Garden, } 20A Inverleith Row, } Edinburgh } EH3 5LR } Tel: 0131 248 2817 } Fax: 0131 248 2901 } E-Mail: F.Christie-at-rbge.org.uk } http://www.rbge.org.uk } } } } } ==============================Original Headers============================== } 9, 26 -- From F.Christie-at-rbge.ac.uk Thu Feb 21 02:01:04 2008 } 9, 26 -- Received: from mailfilter.rbge.org.uk (mailfilter.rbge.org.uk [193.62.154.31]) } 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1L814TS011930 } 9, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 02:01:04 -0600 } 9, 26 -- Received: from mailfilter.rbge.org.uk (localhost.localdomain [127.0.0.1]) } 9, 26 -- by localhost (Email Security Appliance) with SMTP id B82D8164737 } 9, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 07:50:54 +0000 (GMT) } 9, 26 -- Received: from marmaduke.rbge.org.uk (unknown [10.0.20.20]) } 9, 26 -- by mailfilter.rbge.org.uk (Email Security Appliance) with ESMTP id B1220159264 } 9, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 07:50:54 +0000 (GMT) } 9, 26 -- content-class: urn:content-classes:message } 9, 26 -- MIME-Version: 1.0 } 9, 26 -- Content-Type: text/plain; } 9, 26 -- charset="us-ascii" } 9, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6619.12 } 9, 26 -- Subject: Diatom embedding protocol } 9, 26 -- Date: Thu, 21 Feb 2008 08:04:03 -0000 } 9, 26 -- Message-ID: {07478CCB1EF74F49911295DA60CCF5739C2768-at-marmaduke.rbge.org.uk} } 9, 26 -- X-MS-Has-Attach: } 9, 26 -- X-MS-TNEF-Correlator: } 9, 26 -- Thread-Topic: Diatom embedding protocol } 9, 26 -- Thread-Index: Ach0YEh+saP4fQaoQjmZ0Y+SX3BvQw== } 9, 26 -- From: "Frieda Christie" {F.Christie-at-rbge.ac.uk} } 9, 26 -- To: {Microscopy-at-microscopy.com} } 9, 26 -- Content-Transfer-Encoding: 8bit } 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1L814TS011930 } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 22 -- From dac-at-research.umass.edu Thu Feb 21 08:34:37 2008 4, 22 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1LEYbvn012611 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 08:34:37 -0600 4, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 4, 22 -- (authenticated bits=0) 4, 22 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1LEYatm027672 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 09:34:36 -0500 4, 22 -- Message-ID: {47BD8CD3.1060900-at-research.umass.edu} 4, 22 -- Date: Thu, 21 Feb 2008 09:38:11 -0500 4, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 22 -- Reply-To: dac-at-research.umass.edu 4, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 4, 22 -- MIME-Version: 1.0 4, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] Diatom embedding protocol 4, 22 -- References: {200802210809.m1L890Jq020895-at-ns.microscopy.com} 4, 22 -- In-Reply-To: {200802210809.m1L890Jq020895-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From ph2-at-sprynet.com Thu Feb 21 08:56:28 2008 17, 27 -- Received: from elasmtp-scoter.atl.sa.earthlink.net (elasmtp-scoter.atl.sa.earthlink.net [209.86.89.67]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1LEuRmS025968 17, 27 -- for {microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 08:56:27 -0600 17, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 27 -- s=dk20050327; d=sprynet.com; 17, 27 -- b=LQr9KvwCFGV9mE3u2ZvjBy/rBZaIqJaY9pvE3NMdvg81Pb/BTggfckZFmEcJn/aN; 17, 27 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:In-Reply-To:Thread-Index:Message-ID:X-ELNK-Trace:X-Originating-IP; 17, 27 -- Received: from [75.61.18.94] (helo=user915fa8f284) 17, 27 -- by elasmtp-scoter.atl.sa.earthlink.net with asmtp (Exim 4.34) 17, 27 -- id 1JSCqZ-0003KR-0x; Thu, 21 Feb 2008 09:56:27 -0500 17, 27 -- From: "Tony Havics" {ph2-at-sprynet.com} 17, 27 -- To: {dac-at-research.umass.edu} , 17, 27 -- "Microscopy Listserve" {microscopy-at-microscopy.com} 17, 27 -- Subject: RE: [Microscopy] Re: Diatom embedding protocol 17, 27 -- Date: Thu, 21 Feb 2008 09:56:24 -0500 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="US-ASCII" 17, 27 -- Content-Transfer-Encoding: 7bit 17, 27 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 17, 27 -- In-Reply-To: {200802211440.m1LEeQHp020580-at-ns.microscopy.com} 17, 27 -- Thread-Index: Ach0l7N5ZlgXg9EFTOSH+G1qyREOAAAAbQcg 17, 27 -- Message-ID: {E1JSCqZ-0003KR-0x-at-elasmtp-scoter.atl.sa.earthlink.net} 17, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f98f60b437238bb721ef19781af5aaf8a3350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 17, 27 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
I assisted a grad student from Ohio back in the early '70s with pictures for his thesis on diatoms. He discovered a type that was thought to only be in Europe.
The method we used was to lightly dust whole diatoms onto a collodion coated grid. They stuck like dust since the grids were NOT glow discharged. Then put the grids into the TEM.
I believe that most labs studying diatoms now use SEM.
Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} == } I'd be grateful if someone could recommend a protocol for diatom } embedding for TEM sectioning as this material is new to me. } } Frieda Christie, } Electron Microscopist, } Royal Botanic Garden, } 20A Inverleith Row, } Edinburgh } EH3 5LR } Tel: 0131 248 2817 } Fax: 0131 248 2901 } E-Mail: F.Christie-at-rbge.org.uk } http://www.rbge.org.uk
==============================Original Headers============================== 8, 24 -- From connellyps-at-nhlbi.nih.gov Thu Feb 21 09:14:35 2008 8, 24 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1LFEZS6006712 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 09:14:35 -0600 8, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 8, 24 -- Thu, 21 Feb 2008 10:14:31 -0500 8, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.165]) with Microsoft Exchange Server HTTP-DAV ; 8, 24 -- Thu, 21 Feb 2008 15:14:30 +0000 8, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 8, 24 -- Date: Thu, 21 Feb 2008 10:11:40 -0500 8, 24 -- Subject: Re: [Microscopy] Diatom embedding protocol 8, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 8, 24 -- To: {F.Christie-at-rbge.ac.uk} 8, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 24 -- Message-ID: {C3E2FEDC.15EC%connellyps-at-nhlbi.nih.gov} 8, 24 -- Thread-Topic: [Microscopy] Diatom embedding protocol 8, 24 -- Thread-Index: Ach0nA9FTgSR8uCPEdyv+gANk2Yv1A== 8, 24 -- In-Reply-To: {200802210808.m1L88QDj020159-at-ns.microscopy.com} 8, 24 -- Mime-version: 1.0 8, 24 -- Content-type: text/plain; 8, 24 -- charset="ISO-8859-1" 8, 24 -- X-OriginalArrivalTime: 21 Feb 2008 15:14:31.0209 (UTC) FILETIME=[75520990:01C8749C] 8, 24 -- Content-Transfer-Encoding: 8bit 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1LFEZS6006712 ==============================End of - Headers==============================
After an ISP meltdown, back on line I, would like to comment on Jason Saredy's problems.
I believe some of the answers that he has had could be a little misleading, but I will explain. Listers have commented that Jason's filament life (around 50 hours) could be a clue to his problem of not being able to obtain good quality images at 5kV. I feel this is a misunderstanding of the role that the filament plays in performance.
In Microscopy Today, March/April 2003 was an article called The Life and Death of the Tungsten Hairpin Filament, where I discussed the operating advantages and disadvantages that would be stimulated by the filament. In fact it is the position of the filament that varies its contribution towards resolution. The manufacturers generally guide operators to a filament to cathode distance that offers reasonable resolution with a reasonable filament life; the 50 hours that Jason was getting fits in with this criteria, I would say 50 to 60 hours is typical.
For higher performance we move the filament forwards towards the cap, resulting in a reduction in the filament life as it is worked harder to attaining higher emission/beam currents. The higher current enabling smaller spot sizes to be used whilst retaining a reasonable signal level. For longer filament life we move it back from the cathode cap, this reduces the emission/beam current, but will considerable increase filament life to the determent of image quality, just not enough electrons at the specimen level! Whilst I know many instruments that run up to 2,000 hours of filament life they are not used for high quality imaging. However many times in my career have I found the reason for poor performance is the SEM laboratory are not pushing the filament hard enough; you have to have a reasonable emission/beam current (80-120uA) if you want high performance
So if we look at Jason's problem a short filament life would tend to suggest a high emission/beam current! Assuming sensible levels of saturation the other reason for poor filament life would be a poor vacuum in the gun, but a SEM running for 50 hours on a filament would not suggest poor vacuum.
Moving on to his real problem, he is unable to obtain good quality images at 5kV because his stigmators are hard against the stop! When you work at low kV the cleanliness of the column is most important. At high kV the beam is able to penetrate column contamination to find an earth; no problems. Excess astigmatism will be the result if the kV is lowered reducing its ability to penetrate column contamination; producing excess charge.
So how does Jason overcome his problems? Firstly clean the final aperture, if this does not work clean the aperture holder, if this does not work check the ESEM full alignment and still no solution then finally clean the complete column.
A piece of advice to help operators but unfortunately to upset some of my many service engineer friends. When a SEM is serviced a 30kV picture will tell you that the gun is clean and happy, but more importantly in this era of low kV a low kV picture will show that the column is really clean. I have this saying that my dog could clean a column suitable for a good picture at 30kV but it takes a good service to clean the column for 2kV! Sorry service teams!
Happy scanning
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
==============================Original Headers============================== 13, 28 -- From protrain-at-emcourses.com Thu Feb 21 11:15:48 2008 13, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1LHFkWn004071 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 11:15:47 -0600 13, 28 -- Received: from 5ac8bf5d.bb.sky.com ([90.200.191.93]:3379 helo=HP6220) 13, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 13, 28 -- (envelope-from {protrain-at-emcourses.com} ) 13, 28 -- id 1JSF1L-000OdK-Tu by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 21 Feb 2008 19:15:44 +0200 13, 28 -- Message-ID: {000f01c874ad$61fd17c0$0200a8c0-at-HP6220} 13, 28 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com} 13, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 13, 28 -- To: "American Society" {microscopy-at-microscopy.com} 13, 28 -- Subject: [Microscopy] Re: W Filament and ESEM 13, 28 -- Date: Thu, 21 Feb 2008 17:15:24 -0000 13, 28 -- Organization: Protrain 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Type: text/plain; 13, 28 -- format=flowed; 13, 28 -- charset="Windows-1252"; 13, 28 -- reply-type=original 13, 28 -- Content-Transfer-Encoding: 7bit 13, 28 -- X-Priority: 3 13, 28 -- X-MSMail-Priority: Normal 13, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 13, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 13, 28 -- X-Scan-Signature: 3f4edaa198eaf45467af03745bdebb26{67}} 13, 28 -- X-Trace: smtp01.dial-up.net 1JSF1L-000OdK-Tu b1f3cdd1e0ac875463e7b3f702044281 ==============================End of - Headers==============================
I've been asked why I wish to section diatoms so just to clarify, I've been observing diatoms for many years using SEM but a colleague wishes to study the fine processes that occur at a cellular level during the sexual phase of reproduction. It is something of a challenge as you can imagine hence my request to the list server.
Our experience is that the new formulations resolve the issue of brittleness but other problems from the ERL4221 remain. The polymerized plastic swells if contacted with ethanol, such as when cleaning a block to remove dust after sawing it apart, and it has a significantly higher fluorescence in the case of embedding fluorescently labeled tissues.
Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 9, 24 -- From glenmac-at-u.washington.edu Fri Feb 22 12:04:45 2008 9, 24 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1MI4jOh021490 9, 24 -- for {microscopy-at-microscopy.com} ; Fri, 22 Feb 2008 12:04:45 -0600 9, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 9, 24 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m1MI4iIu032073 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 9, 24 -- for {microscopy-at-microscopy.com} ; Fri, 22 Feb 2008 10:04:44 -0800 9, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 9, 24 -- (authenticated authid=glenmac) 9, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m1MI4i2E014350 9, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 9, 24 -- for {microscopy-at-microscopy.com} ; Fri, 22 Feb 2008 10:04:44 -0800 9, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 9, 24 -- Content-Transfer-Encoding: 7bit 9, 24 -- Message-Id: {053028FA-858A-440B-8717-4D68D5DEC691-at-u.washington.edu} 9, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 9, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 9, 24 -- Subject: Re: Spurr's Resin 9, 24 -- Date: Fri, 22 Feb 2008 10:04:43 -0800 9, 24 -- X-Mailer: Apple Mail (2.753) 9, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.2.22.94832 9, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_800_899 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
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Email: david.tanner-at-ul.ie Name: David Tanner
Organization: Materials And Surface Science Institute, University of Limerick
Title-Subject: [Filtered] Postdoctoral Researcher in Electron Microscopy
Question: The Materials & Surface Science Institute (MSSI) was established in 1998 as a large-scale research institute focussed on the development and characterisation of novel materials. The Institute is the largest research institute at the University of Limerick and currently houses 200 researchers. A team of six support personnel supports the activities of the Institute. The research of the Institute is focused on four themes: Nanomaterials, Composite and Glass Materials, Biomaterials and Bio/Catalysis and Clean Technology. MSSI has been successful in obtaining Ä5.8 million to fund research in microscopy, nanomaterials and computational nanomechanics as part of the national consortium on Nanoscience and Nanoscale Technologies for Ireland (NANOTEIRE). As part of this research programme, MSSI is upgrading its existing microsopy facilities (Jeol 2011 TEM and 2 Jeol SEM instruments) by adding FEG-TEM and FEG-SEM instruments, together with ancillary equipment. The existing Kratos XPS is currently being upgraded. Applications are now invited for the following vacancies: (i) Postdoctoral Researcher in Electron Microscopy and (ii) Postdoctoral Researcher in Surface Science. The successful candidates will report to the Director or his nominee and will support the research activities of the Institute. He/she will assume responsibility for the operation and maintenance of specialised equipment and associated facilities in order to ensure an efficient service to satisfy the research requirements of the Institute. Applicants should have a Ph.D. in chemistry/physics/materials science or a relevant discipline. Good communication skills are essential. The successful candidate in electron microscopy should have demonstrable experience of working with transmission electron microscopes. For the surface science post the successful candidate will have demonstrable experience of working with ultrahigh vacuum systems and preferably experience in XPS. The successful candidates must be capable of working independently and of developing independent research projects. Information on MSSI can be obtained at www.ul.ie/mssi. Informal enquiries about the post can be made to Prof. Edmond Magner, Materials & Surface Science Institute, University of Limerick, Limerick, Ireland (Tel: (353) 61 202629, e-mail: edmond.magner-at-ul.ie). The appointments will be made on the postdoctoral researcher scale Ä38,623 ñ 54,774. Please note your application must include: 1) A letter/email of introduction indicating how you meet the criteria outlined in the advertisement and/or information for applicants. 2) A full curriculum vitae. 3) Copies of relevant publications that include evidence of instrumental expertise. The closing date for receipt of completed applications is March 7, 2008. Further information for applicants and application material is available from: Human Resources ñ Email: hr-at-ul.ie ñ Web: http://www.ul.ie/hrvacancies/ Applications are welcome from suitably qualified female and male candidates. The University is an equal opportunities employer and committed to selection on merit.
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Email: thatch-at-mit.edu Name: Tori Hatch
Organization: MIT/HHMI
Title-Subject: [Filtered] black and white print processor
Question: Hello,
I am looking for a black and white print processor to print 8.5 x 10 images. If anyone is still manufacturing these I'd like to know about them, but it appears that they are rapidly becoming dinasaurs. If you have a used machine with reasonably good (or replaceable) rollers to sell please contact me.
Thanks, Tori Hatch Horvitz Lab MIT / HHMI 617-253-6138
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Email: kirktons-at-union.edu Name: Scott Kirkton
Organization: Union College
Title-Subject: [Filtered] Preparing Grasshopper Legs for SEM
Question: Hi-
I am new to SEM and hope you can help.
I would like to measure external diameters of grasshopper legs (width, length) in a SEM. I want to minimize any distortion (shrinkage) but I am having trouble differentiating between various preparation techniques:
1) Air Dry
2) Chemical Fix: GA, OsO4,
3) Critical Point Drying
4) Drying with Organic Solvent: HMDS (Hexamethyldisilazane).
Does anyone have any suggestions for which one to choose? If so, could they recommend a published protocol?
eikonika-at-otenet.gr yorgosnikas-at-hotmail.com Tel/fax +30 210 8957677 Mobile +30 6945 107477
----- Original Message ----- X-from: {kirktons-at-union.edu} To: {eikonika-at-otenet.gr} Sent: Saturday, February 23, 2008 1:13 AM
As my wife asked me when she saw this email:
If you are only measuring the diameter of something this large, why an SEM?
Particularly when you've admitted to having slight distortions by prepping the samples?
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
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-----Original Message----- X-from: kirktons-at-union.edu [mailto:kirktons-at-union.edu] Sent: Friday, February 22, 2008 6:10 PM To: ph2-at-sprynet.com
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Email: kirktons-at-union.edu Name: Scott Kirkton
Organization: Union College
Title-Subject: [Filtered] Preparing Grasshopper Legs for SEM
Question: Hi-
I am new to SEM and hope you can help.
I would like to measure external diameters of grasshopper legs (width, length) in a SEM. I want to minimize any distortion (shrinkage) but I am having trouble differentiating between various preparation techniques:
1) Air Dry
2) Chemical Fix: GA, OsO4,
3) Critical Point Drying
4) Drying with Organic Solvent: HMDS (Hexamethyldisilazane).
Does anyone have any suggestions for which one to choose? If so, could they recommend a published protocol?
DId you ask around the Plant Scientists in the biology Department?
Secondly, if you can not find a slideing microtome (the ideal solution) here something to try. I had a student digitzing and measuring rings from tree cores, in order to get enough clarity of the rings we used a hand plane (the kind you would use for wood working), with a well sharpened and honed blade. He made a tray for holding the cores horizontally. In your case you should be able to make a jig for holding the stems and running a plane across the end. pick up the slices on a glass slide and go from there.
On 19 Feb 2008 at 17:57, 2kjc2-at-queensu.ca wrote:
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Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 9, 25 -- From edelmare-at-muohio.edu Sat Feb 23 14:15:42 2008 9, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1NKFgoI001721 9, 25 -- for {microscopy-at-Microscopy.com} ; Sat, 23 Feb 2008 14:15:42 -0600 9, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 9, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m1NKFgoa026812; 9, 25 -- Sat, 23 Feb 2008 15:15:42 -0500 9, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 9, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m1NKFffL020855; 9, 25 -- Sat, 23 Feb 2008 15:15:41 -0500 9, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 9, 25 -- To: "2kjc2-at-queensu.ca" {2kjc2-at-queensu.ca} 9, 25 -- Date: Sat, 23 Feb 2008 15:15:41 -0500 9, 25 -- MIME-Version: 1.0 9, 25 -- Subject: Re: [Microscopy] viaWWW: Sliding microtome? 9, 25 -- CC: microscopy-at-Microscopy.com 9, 25 -- Message-ID: {47C0389D.20955.5848166-at-edelmare.muohio.edu} 9, 25 -- Priority: normal 9, 25 -- In-reply-to: {200802192257.m1JMv666011566-at-ns.microscopy.com} 9, 25 -- References: {200802192257.m1JMv666011566-at-ns.microscopy.com} 9, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 25 -- Content-type: text/plain; charset=US-ASCII 9, 25 -- Content-transfer-encoding: 7BIT 9, 25 -- Content-description: Mail message body 9, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
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Question: during plant (leaf) tissue fixation, a protocol will often recommend application of vacuum to de-gas the tissue and cause it to fully submerge in the fixative. What level of vacuum is required to cause de-gassing of leaf material (approximately)? Our lab has a 'house' vacuum line but the level of vacuum it provides is not great, perhaps 0.25-0.5 atm max. When attempting to de-gas leaf tissue, this level of vac is not sufficient, at least not during a standard 2 hr fix period. On the opposite end of the spectrum, I don't want to overdo it and cause fixative to evaporate by supplying too great of a vacuum level. Does anyone have a value or range for appropriate vac level for this purpose? Thank you
Can anyone tell me what are contained in the Serial connection kit (PW6443/00) and the Remote control function (PW6460/00)?
We have a Philips CM100 TEM and I am trying to figure out how to get the remote control function to work. I found the CM Monitor and Remote control software (PW6470/40, rcm.sys driver and cmonitor.exe) but not anything on the Serial connection kit (PW6443/00), and the Remote control function (PW6460/00).
I am hoping to use the software to monitor the machine because it keeps shutting itself down after 2-3 days. All air and water flow seems to be fine, and vacuum appears to be normal except P2 is rather high (~78). The other major problem is that the wehnelt S.W. protection on the high tension buffer board is tripped right from startup and cannot be rectified by pushing the reset button; so high tension cannot be switch on.
I plan to call FEI next week but just hope that someone on the list is familiar with the cm100 to shed some light on the remote monitor function and the wehnelt S.W. protection reset. Thanks a lot.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
==============================Original Headers============================== 6, 20 -- From wpchan-at-u.washington.edu Sat Feb 23 15:27:54 2008 6, 20 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1NLRrZ8021026 6, 20 -- for {microscopy-at-microscopy.com} ; Sat, 23 Feb 2008 15:27:53 -0600 6, 20 -- Received: from homer24.u.washington.edu (homer24.u.washington.edu [140.142.15.10]) 6, 20 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m1NLRrqU010655 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 20 -- for {microscopy-at-microscopy.com} ; Sat, 23 Feb 2008 13:27:53 -0800 6, 20 -- Received: from localhost (wpchan-at-localhost) 6, 20 -- by homer24.u.washington.edu (8.13.7+UW06.06/8.13.7+Submit) with ESMTP id m1NLRqD9016237 6, 20 -- for {microscopy-at-microscopy.com} ; Sat, 23 Feb 2008 13:27:52 -0800 6, 20 -- Date: Sat, 23 Feb 2008 13:27:52 -0800 (PST) 6, 20 -- From: "W. Chan" {wpchan-at-u.washington.edu} 6, 20 -- To: microscopy-at-microscopy.com 6, 20 -- Subject: cm100 remote control 6, 20 -- Message-ID: {Pine.LNX.4.64.0802231229080.9631-at-homer24.u.washington.edu} 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 6, 20 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.2.23.131430 6, 20 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
For cleaning tissue from medical device explants, we use a product called Endozyme AW. It is an enzyme product that breaks down organic tissue. We have not had problems with any corrosion reactions for the devices with which we have used this product. The Alconox and Micro-90 products previously suggested are good for final cleaning and should be safe for the burrs, but will not readily break down dried tissue. (Alconox and Micro-90 can corrode some metals, including aluminum and zinc, so use with some caution.) As you now know, anything with chlorine should be avoided to prevent corrosion of the metal.
Good luck.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
} Hi, All- } } I was recently asked to image some surgical burrs (tiny, } diamond-chip-studded drill bits) with SEM. Two were heavily coated with } tissue debris. I successfully cleaned one off with chlorine bleach, but } the other corroded into an ugly mess. This type of project is outside my } range of experience (I tend to avoid clinical stuff). (I know how to get } tissue off coral.) } } More of these burrs are coming soon. Does anyone have any suggestions for } cleaning these burrs while maintaining the integrity of the steel, } adhesive, and diamond chips? } } Aloha from sunny, warm, etc. } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } } **************************************************************************** }
==============================Original Headers============================== 7, 24 -- From hanke-at-mee-inc.com Sun Feb 24 12:16:19 2008 7, 24 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1OIGJ1p013683 7, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 24 Feb 2008 12:16:19 -0600 7, 24 -- Received: (qmail 21605 invoked by uid 508); 24 Feb 2008 12:16:19 -0600 7, 24 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 7, 24 -- (clamdscan: 0.87/5948. spamassassin: 3.0.2. perlscan: 1.24-st-qms. 7, 24 -- Clear:RC:1(216.14.180.82):. 7, 24 -- Processed in 0.082362 secs); 24 Feb 2008 18:16:19 -0000 7, 24 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 7, 24 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.082362 secs Process 21599) 7, 24 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 7, 24 -- by mail.namisolutions.com with SMTP; 24 Feb 2008 12:16:19 -0600 7, 24 -- Message-ID: {47C1B46E.2040109-at-mee-inc.com} 7, 24 -- Date: Sun, 24 Feb 2008 12:16:14 -0600 7, 24 -- From: Larry Hanke {hanke-at-mee-inc.com} 7, 24 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 7, 24 -- MIME-Version: 1.0 7, 24 -- To: Microscopy-at-MSA.Microscopy.Com, tina-at-pbrc.hawaii.edu 7, 24 -- Subject: Re: [Microscopy] RE: Cleaning of surgical burrs for SEM 7, 24 -- References: {200802141906.m1EJ6tr3009163-at-ns.microscopy.com} 7, 24 -- In-Reply-To: {200802141906.m1EJ6tr3009163-at-ns.microscopy.com} 7, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: ewgroth-at-gmail.com Name: Eric W. Roth
Organization: NYUMC Skirball Institute for Biomolecular Medicine EM Core
Title-Subject: [Filtered] anti-HA antibody?
Question: Hello All,
We are going to try to detect HA tags via paraformaldehyde fixed LK4M embedded samples with immuno electron microscopy. Does anyone have a suggestion for a good anti-HA antibody?
My collegues from the TEM told me this morning that they are looking for a second hand CCD camera to be installed on our Topcon 02B TEM, operating at 200 keV.
There was last week a mail offering such a camera installed on a jeol TEM, if I remember right, but I throw the mail away a bit fast ! So I havn't the adress any more.
If this camera is still avaible, could you contact directly my collegue : ulhaq-at-ipcms.u-strasbg.fr.
Any other proposition is welcome too !
Thanks
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Hi, We're just starting out on a project that requires pre-embedding staining for immunocytochem; the specimens are going to be embedded in Epoxy and sectioned for light microscopy; eventually, we hope to extend the technique to pre-embedding immunocytochem for TEM. The specimens are small worms about 500µm by 80µm, and fairly permeable (no cuticle). We have good protocols for double-antibody technique in wholemounts using fluorescent-labeled second antibodies. We'd like to extend the protocol to pre-embedding labeling using enzyme-coupled second antibodies. What are my substrate options for HRP, both to give a label visible in the light-microscopic sections, and ultimately, to give an electron-dense label for TEM? Is there a better choice than HRP? Obviously, we also need a label that will survive ethanol/propylene oxide/epoxy embedding. Julian -- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
The Graduate Center for Materials Research at the Missouri University of Science and Technology (formerly known as the University of Missouri Rolla) is seeking applicants for a research post-doctoral fellow with primary responsibility of TEM sample preparation and analysis, especially for thin films and hard materials. A Helios Nanolab 600 FIB is available for sample preparation, as well as traditional TEM preparation equipment. Ability to utilize other materials characterization techniques such as Auger and X-Ray Photoelectron Spectroscopy, Raman Spectroscopy, and scanning electron microscopy is also desired. Experience in the characterization of interfaces is also beneficial, though not required. Candidate must be able to work independently and in a group, have excellent communication skills, and provide guidance to team members. Project is a part of a multi-university research initiative on diamond coatings.
Qualifications: Ph.D. degree in materials science or related field with extensive background in TEM. Candidates with FIB experience will be given preference. A minimum of 2 years of experience operating a TEM, analysis of images and electron diffraction results, and preparation of TEM samples is required.
Salary Range: Compensation will be commensurate with qualifications and experience.
Documents that MUST be attached by the applicant: Resume/Curriculum Vitae Cover Letter/Letter of Intent References
Contact: F. Scott Miller 223 McNutt Hall Missouri University of Science & Technology 1400 N. Bishop Ave. Rolla, MO 65409 USA Email: smiller-at-umr.edu
Or
Matt O¹Keefe 101 Straumanis Hall Missouri University of Science & Technology 401 W. 16th Street Rolla, MO 65409 USA Email:mjokeefe-at-mst.edu
Open Search: Feb. 20, 2008 Close Search: Until filled, applications reviewed beginning March 1, 2008
==============================Original Headers============================== 6, 25 -- From smiller-at-mst.edu Mon Feb 25 08:50:59 2008 6, 25 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1PEoxJ7021538 6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Feb 2008 08:50:59 -0600 6, 25 -- Received: from exproto2.srv.mst.edu ([131.151.244.4]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 25 -- Mon, 25 Feb 2008 08:50:59 -0600 6, 25 -- Received: from MST-VMAIL3.srv.mst.edu ([131.151.1.193]) by exproto2.srv.mst.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 25 -- Mon, 25 Feb 2008 08:50:58 -0600 6, 25 -- Received: from 131.151.111.190 ([131.151.111.190]) by MST-VMAIL3.srv.mst.edu ([131.151.1.203]) via Exchange Front-End Server minermail.umr.edu ([131.151.0.192]) with Microsoft Exchange Server HTTP-DAV ; 6, 25 -- Mon, 25 Feb 2008 14:50:58 +0000 6, 25 -- User-Agent: Microsoft-Entourage/11.3.6.070618 6, 25 -- Date: Mon, 25 Feb 2008 08:50:57 -0600 6, 25 -- Subject: TEM post-doctoral fellow 6, 25 -- From: Scott Miller {smiller-at-umr.edu} 6, 25 -- To: {Microscopy-at-microscopy.com} 6, 25 -- CC: "OKeefe, Matt" {mjokeefe-at-mst.edu} 6, 25 -- Message-ID: {C3E831F1.1AE00%smiller-at-umr.edu} 6, 25 -- Thread-Topic: TEM post-doctoral fellow 6, 25 -- Thread-Index: Ach3vdQJEkznZOOxEdybygADk71/Mg== 6, 25 -- Mime-version: 1.0 6, 25 -- Content-type: text/plain; 6, 25 -- charset="ISO-8859-1" 6, 25 -- X-OriginalArrivalTime: 25 Feb 2008 14:50:58.0694 (UTC) FILETIME=[D50C5A60:01C877BD] 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1PEoxJ7021538 ==============================End of - Headers==============================
I got my anti-HA from Roche and it has been working really well. (https://www.roche-applied-science.com/servlet/RCConfigureUser?URL=Store FramesetView&storeId=10202&catalogId=10202&langId=-1&countryId=us)
Make sure to choose the right clone for you.
Zhaojie
Zhaojie Zhang, Ph.D. Director, Microscopy Core Facility Department of Zoology and Physiology University of Wyoming Laramie, WY 82071 TEL: 307-766-3038 FAX: 307-766-5625 zzhang-at-uwyo.edu http://www.uwyo.edu/microscopy
-----Original Message----- X-from: ewgroth-at-gmail.com [mailto:ewgroth-at-gmail.com] Sent: Sunday, February 24, 2008 9:04 PM To: Z.J. Zhang
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both ewgroth-at-gmail.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: ewgroth-at-gmail.com Name: Eric W. Roth
Organization: NYUMC Skirball Institute for Biomolecular Medicine EM Core
Title-Subject: [Filtered] anti-HA antibody?
Question: Hello All,
We are going to try to detect HA tags via paraformaldehyde fixed LK4M embedded samples with immuno electron microscopy. Does anyone have a suggestion for a good anti-HA antibody?
This Question was submitted to Ask-A-Microscopist by (mcaruso2-at-uiuc.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, February 6, 2008 at 17:31:31 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both mcaruso2-at-uiuc.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: mcaruso2-at-uiuc.edu Name: Mary M Caruso
Organization: University of Illinois at Urbana-Champaign
Education: Graduate College
Location: Urbana, IL, USA
Title: Stain for imaging amines in epoxy resin
Question: Do you know of a stain for electron microscopy that is functional group specific? We would like to add a stain or dye that would tag amines in an epoxy matrix to show that some of them have not completely reacted in the 3D polymer network. I tried ninhydrin as a stain and can see the purple color appear on the surface of the resin but imaging these were more difficult. Thank you for your help. ~Mary
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (releech-at-telusplanet.net) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, February 25, 2008 at 09:56:04 ---------------------------------------------------------------------------
Email: releech-at-telusplanet.net Name: Robin Leech
Organization: Alberta Society of Professional Biologists
Education: Graduate College
Location: Edmonton, Alberta, Canada
Question: I have recently purchased an AO Spencer Stereo Microscope, made ca. 1955, with a polarizing function on it. I am interested in examining the eyes of spiders to check for spiders' abilities to use polarized light for aerial (ballooning) navigation. Thus, I am interested in polarized light that is both transmitted and reflected. There are no instructions with the polarizer. Does anyone have instructions that I may copy or purchase? Or, can someone give me some instructions?
Dear All I am new to the materials world so please excuse my ignorance on the following subject. A colleague wants to take a cross section of a polymer film that consists of 2 thin layers (in order to demonstrate the layering of the material). Both polymer layers are on the order of 15 nm thick and can only grown on silicon wafer or mica for some reason. They also can not be taken above/below 35/-20 centigrade respectively nor can they be put in many organic solvents. The question was has anyone had any experience of visualising the layers of films like these using EM? I have read some posts on films but they all seem to use unsuitable conditions or are for larger films. Any advice would be great fully received. Regards John
==============================Original Headers============================== 1, 27 -- From john.mitchels-at-gmail.com Mon Feb 25 12:56:37 2008 1, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1PIuaBk016711 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Feb 2008 12:56:36 -0600 1, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1581823rvb.30 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Feb 2008 10:56:35 -0800 (PST) 1, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 27 -- d=gmail.com; s=gamma; 1, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- bh=LPfWVVMx4QXO0wNPHNKWEsYDs5U0sIbORpakq+cXV1k=; 1, 27 -- b=Br2jPrc3ayIlHeargDahmTLchxZ+pru/EBQVJoQk4VMrFPj6OYBqtKfhjK5DWzlFZ8BM9O4+C8A3CB9xObFatnK8IrbzVF/88qRsH6iOfeB8s4LV97bVzX9h4J85WDjfr6MukUrRQA4Iffmi4Sf+i0S0aWmqGMMYxJQVS5or6wE= 1, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 27 -- d=gmail.com; s=gamma; 1, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- b=frHR34kDQCJ8oyLmsNLqec3LSiLFD15kS+HjTdO4Ofu1sSRqXCDn6/Z6ebuYKvNGiwhZWZ6eKWeUb6NHFBWYdA0tHobvUT4RQ/3bObbJfhmsyXXtKyPC0egmovMnoQfvQFldypjc7QADHeFt+E9W8A/7Fc4b9ScYlowc7WeA8yI= 1, 27 -- Received: by 10.141.44.13 with SMTP id w13mr2405101rvj.181.1203965795228; 1, 27 -- Mon, 25 Feb 2008 10:56:35 -0800 (PST) 1, 27 -- Received: by 10.141.33.4 with HTTP; Mon, 25 Feb 2008 10:56:35 -0800 (PST) 1, 27 -- Message-ID: {1b3cf7c30802251056k42c60b95x97fa4073e36603dd-at-mail.gmail.com} 1, 27 -- Date: Mon, 25 Feb 2008 18:56:35 +0000 1, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 1, 27 -- To: Microscopy-at-microscopy.com 1, 27 -- Subject: CS of nm scale thin films 1, 27 -- MIME-Version: 1.0 1, 27 -- Content-Type: text/plain; charset=ISO-8859-1 1, 27 -- Content-Transfer-Encoding: 7bit 1, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I suggest that you try staining with osmium tetroxide according to one of the methods listed in the reference below. I have limited experience with the staining of amines in polymers. However, I do have a hardcopy document that lists a dozen pages of stains that may be used with various polymer systems. As I recall, the document was given to me at M&M 2007 by someone from either the Tech Forum or the Texas Society for Microscopy (http://www.texasmicroscopy.org/). It is entitled Stains for Polymers and includes staining applications for optical microscopy, laser scanning confocal microscopy and TEM. The only reference that I have for the document is a copyright for the 2006 Rubber Division, American Chemical Society. My document refers readers to JMS, 20, 3439 (1985) for osmium staining methods for epoxies with amines. You might also try Polymer Microscopy by Sawyer and Grubbs.
Hope you find what you need.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
mcaruso2-at-uiuc. edu To gary.m.brown-at-exxonmobil.com 02/25/08 12:21 cc PM Subject [Microscopy] AskAMicroscopist: Stain Please respond for imaging amines in epoxy resin to mcaruso2-at-uiuc. edu
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Email: mcaruso2-at-uiuc.edu Name: Mary M Caruso
Organization: University of Illinois at Urbana-Champaign
Education: Graduate College
Location: Urbana, IL, USA
Title: Stain for imaging amines in epoxy resin
Question: Do you know of a stain for electron microscopy that is functional group specific? We would like to add a stain or dye that would tag amines in an epoxy matrix to show that some of them have not completely reacted in the 3D polymer network. I tried ninhydrin as a stain and can see the purple color appear on the surface of the resin but imaging these were more difficult. Thank you for your help. ~Mary
Here is the March 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Friday, February 28, 2008.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor ======================== Microscopy used to Show how Spiders Hide! Stephen W. Carmichael, Mayo Clinic
Improving EDS For Low Energy X-Rays Under 1000eV Using an Attachable Detector Optic David O’Hara, Greg Brown, Eric Lochner, Parallax Research, Inc., Tallahassee, FL
Unique Minilens Cooling System Results in a Compact TEM Dmitry Lysenkov[1], and William C. Monigle,[2]Carl Zeiss SMT AG, Nano Technology Systems Division,[1]Oberkochen, Germany, and [2]Peabody, MA
Simplification and Variation in TEM Focus Techniques Steve Chapman, Protrain, Buckingham, England
Extending Depth of Field in LC-SEM Scenes by Partitioning Sharpness Transforms H. Hariharan*, A. Koschan*, B. Abidi*, D. Page*, M. Abidi*, J. Frafjord** and S. Dekanich**, *IRIS Laboratory, University of Tennessee, Knoxville, TN, **Y12 National Security Complex, Oak Ridge, TN
Nanoparticle Measurement Through Visualisation Bob Carr and Andrew Malloy, NanoSight, Ltd. Wiltshire, U.K.
Microscopy and Microanalysis of Corona Textures in Eclogitic Greenschists from the Eastern Alps, Austria Robert Sturm. Salzburg, Austria
Wide Field and Deep Focus imaging in Photomicrography Optical and Software-Based Techniques Jörg Piper, Clinic Meduna, Bad Bertrich, Germany
A Simple Method for Imaging DNA using SEM N. Chatterjee, K. Andresen, M. Thomas, L. Pollack, E. Kirkland, Cornell University, Ithaca NY
Passive Mirror Imaging through a Solid-State Back-Scattered Electron Detector Fabrizio Croccolo and Claudia Riccardi, Dipartimento di Fisica “G. Occhialini” and PLASMAPROMETEO, Università degli Studi di Milano - Bicocca
Modified Cryo-Preparation for Studying Salt Glands in the Turf Grass Zoysia matrella Sheetal Rao, Michael W. Pendleton, Marla L. Binzel and E. Ann Ellis, Texas A&M University, College Station, TX
Electron Microscopy Vacuum Pump Basics Howard Tring, Vacuum and Low pressure Consulting, Spring City, PA
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kilroth100-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kilroth100-at-gmail.com Name: Andrew Gillies
Organization: Student
Title-Subject: [Filtered] Disinfectants
Question: I would like to know why using disinfectants may be a bad idea. What are the downsides to sterilizing everything? If you could respond with thoughts and other resources I could look at, that would be greatly appreciated.
We have a few copies of Optimizing Light Microscopy left and there is a good intro chapter in there on Pol that you will find very helpful. Contact me off line for ordering details.
Thanks, Barbara Foster
Barbara Foster, President
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
==============================Original Headers============================== 8, 24 -- From bfostermme-at-sbcglobal.net Mon Feb 25 17:45:40 2008 8, 24 -- Received: from smtp113.sbc.mail.mud.yahoo.com (smtp113.sbc.mail.mud.yahoo.com [68.142.198.212]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1PNjdVc011179 8, 24 -- for {microscopy-at-microscopy.com} ; Mon, 25 Feb 2008 17:45:40 -0600 8, 24 -- Message-Id: {200802252345.m1PNjdVc011179-at-ns.microscopy.com} 8, 24 -- Received: (qmail 43692 invoked from network); 25 Feb 2008 23:45:39 -0000 8, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 24 -- s=s1024; d=sbcglobal.net; 8, 24 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:In-Reply-To:References:Mime-Version:Content-Type; 8, 24 -- b=4Qymr6H9l4ydeBhk3s+Eat51awdglnMNp12e0CBmvwF1g2me3EAlJc8sHMgy5/4H97ocwZxgQEsffTsUZBCPv9M7KTFLSFs6RRfDgZADQsaFsTeUyIgbFU+K9hnXcn8HuIboBSyHiZm0h7w4cK1Q9NNoGYB+rEe3EOVKUXjtRQY= ; 8, 24 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-sbcglobal.net-at-68.94.19.176 with login) 8, 24 -- by smtp113.sbc.mail.mud.yahoo.com with SMTP; 25 Feb 2008 23:45:39 -0000 8, 24 -- X-YMail-OSG: iyDHa_wVM1nOrN3KZi2cnqMy3.SeKwcibvh2ilhRq.YlCmmJwGhFPnV_REdwjAuxZb6Q62GUK5ga0LWd8OjTfEU4MiwjQr2_XrEoZrHz7DQS07DheGE- 8, 24 -- X-Yahoo-Newman-Property: ymail-3 8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 24 -- Date: Mon, 25 Feb 2008 17:43:23 -0600 8, 24 -- To: microscopy-at-microscopy.com 8, 24 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 8, 24 -- Subject: Re: [Microscopy] AskAMicroscopist: AO Spencer Stereo 8, 24 -- Microscope Instructions 8, 24 -- In-Reply-To: {200802251830.m1PIUU7J011824-at-ns.microscopy.com} 8, 24 -- References: {200802251830.m1PIUU7J011824-at-ns.microscopy.com} 8, 24 -- Mime-Version: 1.0 8, 24 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Me too - and following my first attempt and the drastic affect on filament life I look forward to seeing your replies.
Sectioning went well (using Spurr as a supporting resin) but the sample was severely affected by beam damage (CM10; 80Kv) with the filament breaking a few days after viewing. Even after taking precautions to protect the section (formvar) and careful use of the beam intensity, a second and third filament broke shortly after examination.
I have just confirmed with FEI that fitting a cold trap would largely overcome this problem so I am suspending further viewing till I can get further details on this.
I would be grateful if any listers could confirm (or contradict) this hoped for solution and if you could please pass on any replies that have not included Microscopy-at-microscopy.com in the reply.
Best regards,
Alastair
Alastair McKinnon Histology & EM Facility Manager, IMS R2.62 University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] Sent: 25 February 2008 19:01 To: Mckinnon, Alastair D.
Dear All I am new to the materials world so please excuse my ignorance on the following subject. A colleague wants to take a cross section of a polymer film that consists of 2 thin layers (in order to demonstrate the layering of the material). Both polymer layers are on the order of 15 nm thick and can only grown on silicon wafer or mica for some reason. They also can not be taken above/below 35/-20 centigrade respectively nor can they be put in many organic solvents. The question was has anyone had any experience of visualising the layers of films like these using EM? I have read some posts on films but they all seem to use unsuitable conditions or are for larger films. Any advice would be great fully received. Regards John
==============================Original Headers============================== 1, 27 -- From john.mitchels-at-gmail.com Mon Feb 25 12:56:37 2008 1, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1PIuaBk016711 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Feb 2008 12:56:36 -0600 1, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1581823rvb.30 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Feb 2008 10:56:35 -0800 (PST) 1, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 27 -- d=gmail.com; s=gamma; 1, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- bh=LPfWVVMx4QXO0wNPHNKWEsYDs5U0sIbORpakq+cXV1k=; 1, 27 -- b=Br2jPrc3ayIlHeargDahmTLchxZ+pru/EBQVJoQk4VMrFPj6OYBqtKfhjK5DWzlFZ8BM9O 4+C8A3CB9xObFatnK8IrbzVF/88qRsH6iOfeB8s4LV97bVzX9h4J85WDjfr6MukUrRQA4Iff mi4Sf+i0S0aWmqGMMYxJQVS5or6wE= 1, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 27 -- d=gmail.com; s=gamma; 1, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer-encoding:content-disposition; 1, 27 -- b=frHR34kDQCJ8oyLmsNLqec3LSiLFD15kS+HjTdO4Ofu1sSRqXCDn6/Z6ebuYKvNGiwhZWZ 6eKWeUb6NHFBWYdA0tHobvUT4RQ/3bObbJfhmsyXXtKyPC0egmovMnoQfvQFldypjc7QADHe Ft+E9W8A/7Fc4b9ScYlowc7WeA8yI= 1, 27 -- Received: by 10.141.44.13 with SMTP id w13mr2405101rvj.181.1203965795228; 1, 27 -- Mon, 25 Feb 2008 10:56:35 -0800 (PST) 1, 27 -- Received: by 10.141.33.4 with HTTP; Mon, 25 Feb 2008 10:56:35 -0800 (PST) 1, 27 -- Message-ID: {1b3cf7c30802251056k42c60b95x97fa4073e36603dd-at-mail.gmail.com} 1, 27 -- Date: Mon, 25 Feb 2008 18:56:35 +0000 1, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 1, 27 -- To: Microscopy-at-microscopy.com 1, 27 -- Subject: CS of nm scale thin films 1, 27 -- MIME-Version: 1.0 1, 27 -- Content-Type: text/plain; charset=ISO-8859-1 1, 27 -- Content-Transfer-Encoding: 7bit 1, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 16, 24 -- From a.d.mckinnon-at-abdn.ac.uk Tue Feb 26 05:05:21 2008 16, 24 -- Received: from mailhub2.abdn.ac.uk (mailhub2.abdn.ac.uk [139.133.7.24]) 16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QB5Km4015042 16, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 05:05:20 -0600 16, 24 -- Received: from ew-mail-a.uoa.abdn.ac.uk ([139.133.15.20] helo=VMAIL1.uoa.abdn.ac.uk) 16, 24 -- by mailhub2.abdn.ac.uk with esmtp (Exim 4.52) 16, 24 -- id 1JTxce-0005ta-0c; Tue, 26 Feb 2008 11:05:20 +0000 16, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 24 -- Content-class: urn:content-classes:message 16, 24 -- MIME-Version: 1.0 16, 24 -- Content-Type: text/plain; 16, 24 -- charset="us-ascii" 16, 24 -- Subject: RE: [Microscopy] CS of nm scale thin films 16, 24 -- Date: Tue, 26 Feb 2008 10:55:21 -0000 16, 24 -- Message-ID: {009B2FCFB3DD25408A4BBC240F091376012AE74B-at-VMAIL1.uoa.abdn.ac.uk} 16, 24 -- X-MS-Has-Attach: 16, 24 -- X-MS-TNEF-Correlator: 16, 24 -- Thread-Topic: [Microscopy] CS of nm scale thin films 16, 24 -- thread-index: Ach34FmJkpZHj+LMR2i0S4+LAcK9GQAe6JOg 16, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} 16, 24 -- To: {john.mitchels-at-gmail.com} 16, 24 -- Cc: {Microscopy-at-microscopy.com} 16, 24 -- Content-Transfer-Encoding: 8bit 16, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1QB5Km4015042 ==============================End of - Headers==============================
Again it looks like we can get the money to upgrade our EM lab. I need advice on buying a digital camera for our CM12 TEM. Usual magnifications 1k to 100 k, occasionally higher. The usual application is biology, occasionally materials (with diffraction) and nanoparticles.
Should I go for a bottom or a side mounted camera (I am still afraid to be left without film with a bottom mounted camera)?
What is more important - pixel size or pixel count?
What about dynamic range - is 12 bit acceptable or should I go for 16 bit?
Any other recommendations?
Any comments on existing equipment are very welcome off-list.
Thank you,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
We have inherited an early version Nikon Epiphot(-TME) microscope in good working order. It has all kinds of filtering gadgets, some I'm not familiar with. If anyone has, or knows where I can get, a manual for this microscope I'd be very grateful. We would certainly pay for it, or a copy of it. Please contact me off-line if you have any info.
Thank you,
Jane L. LaGoy Lab Services Mgr./Development Engr. Bodycote HIP 155 River St. Andover, MA 01810 978-470-1620 x450 jane.lagoy-at-bodycote.com
This Email and all attachments hereto, if any, are covered by the provisions of the U.S. Electronic Communications Privacy Act. Additionally, all contents of this communication and all attachments hereto shall be deemed to be confidential and the contents thereof are proprietary to Bodycote Thermal Processing, Inc. ("Bodycote"). If this communication has been sent to you in error, please reply to the sender that you received it and then delete the message immediately. Any dissemination, distribution, copying or reproduction of this message other than by its intended recipient is strictly prohibited.
==============================Original Headers============================== 7, 31 -- From Jane.LaGoy-at-Bodycote.com Tue Feb 26 10:19:53 2008 7, 31 -- Received: from mail144.messagelabs.com (mail144.messagelabs.com [216.82.254.51]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1QGJq5Z020651 7, 31 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 26 Feb 2008 10:19:52 -0600 7, 31 -- X-VirusChecked: Checked 7, 31 -- X-Env-Sender: Jane.LaGoy-at-Bodycote.com 7, 31 -- X-Msg-Ref: server-2.tower-144.messagelabs.com!1204042791!23559393!1 7, 31 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- 7, 31 -- X-Originating-IP: [209.235.7.177] 7, 31 -- Received: (qmail 3523 invoked from network); 26 Feb 2008 16:19:52 -0000 7, 31 -- Received: from 177-209.235.7.appsitehosting.com (HELO VM200EXC02.ad.bodycote.na) (209.235.7.177) 7, 31 -- by server-2.tower-144.messagelabs.com with SMTP; 26 Feb 2008 16:19:52 -0000 7, 31 -- Received: from VM200EXC01.ad.bodycote.na ([10.200.20.9]) by VM200EXC02.ad.bodycote.na with Microsoft SMTPSVC(6.0.3790.3959); 7, 31 -- Tue, 26 Feb 2008 11:19:49 -0500 7, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4133 7, 31 -- Content-Class: urn:content-classes:message 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; 7, 31 -- charset="US-ASCII" 7, 31 -- Subject: manual for Epiphot or Epiphot-TME 7, 31 -- Date: Tue, 26 Feb 2008 11:19:52 -0500 7, 31 -- Message-ID: {412949C8D462414B9BE7A0921A9C841636B8ED-at-VM200EXC01.ad.bodycote.na} 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- Thread-Topic: manual for Epiphot or Epiphot-TME 7, 31 -- thread-index: Ach4k2pl8fmY4m6GRiqsiA4jJShDSg== 7, 31 -- From: "Jane LaGoy" {Jane.LaGoy-at-Bodycote.com} 7, 31 -- To: {Microscopy-at-MSA.Microscopy.com} 7, 31 -- X-OriginalArrivalTime: 26 Feb 2008 16:19:49.0895 (UTC) FILETIME=[691AD170:01C87893] 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1QGJq5Z020651 ==============================End of - Headers==============================
ICEMS Instituto de Ciência e Engenharia de Materiais e Superfícies of Instituto Superior Técnico, Technical University of Lisbon (www.icems.ist.utl.pt {http://www.icems.ist.utl.pt} ) has a free researcher position in characterisation of thin films and surfaces by cross-sectional transmission electron microscopy. The contract offered will have duration of up to 5 years, renewed yearly on mutual agreement.
The candidates must have a PhD degree in materials science, physics or a related field and more than 2 years of research experience after the PhD and 3 years experience operating a TEM. The candidate must be experienced in the characterisation of materials and thin films by transmission electron microscopy/cross-sectional TEM. Experience in TEM sample preparation for cross-sectional TEM analysis of thin films is desirable as well as the ability to use other materials characterization techniques such as SEM/EBSD/EDS and Auger electron spectroscopy.
The researcher should be willing to work as part of a large team, but is also expected to conduct independent research and generate and manage his own projects. The candidate must provide guidance in TEM to other team members and PhD students.
The applicants must submit the following documents for appreciation:
Curriculum Vitae Letter of Intent References
Contact: Prof. Rui Vilar Instituto Superior Técnico Av. Rovisco Pais 1049-001 Lisboa, Portugal Tel: +351-218418121 fax: +351-218418120 Email: rui.vilar-at-ist.utl.pt
==============================Original Headers============================== 6, 32 -- From rui.vilar-at-ist.utl.pt Tue Feb 26 12:27:22 2008 6, 32 -- Received: from smtp1.ist.utl.pt (smtp1.ist.utl.pt [193.136.128.21]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QIRLHh005897 6, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 12:27:21 -0600 6, 32 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 6, 32 -- by smtp1.ist.utl.pt (Postfix) with ESMTP id 5A36370004AE 6, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 18:27:18 +0000 (WET) 6, 32 -- X-Virus-Scanned: by amavisd-new-2.4.2 (20060627) (Debian) at ist.utl.pt 6, 32 -- Received: from smtp1.ist.utl.pt ([127.0.0.1]) 6, 32 -- by localhost (smtp1.ist.utl.pt [127.0.0.1]) (amavisd-new, port 10025) 6, 32 -- with LMTP id 7M05aBGg67dA for {Microscopy-at-microscopy.com} ; 6, 32 -- Tue, 26 Feb 2008 18:27:18 +0000 (WET) 6, 32 -- Received: from mail.ist.utl.pt (mail.ist.utl.pt [193.136.128.8]) 6, 32 -- by smtp1.ist.utl.pt (Postfix) with ESMTP id 28770700042F 6, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 18:27:18 +0000 (WET) 6, 32 -- Received: from [193.136.139.205] (mat5.civil.ist.utl.pt [193.136.139.205]) 6, 32 -- (Authenticated sender: ist12729-at-mail.ist.utl.pt) 6, 32 -- by mail.ist.utl.pt (Postfix) with ESMTP id DE5CC1400320 6, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 18:27:17 +0000 (WET) 6, 32 -- User-Agent: Microsoft-Entourage/11.3.6.070618 6, 32 -- Date: Tue, 26 Feb 2008 18:26:31 +0000 6, 32 -- Subject: Electron microscopy position 6, 32 -- From: Rui Vilar {rui.vilar-at-ist.utl.pt} 6, 32 -- To: {Microscopy-at-microscopy.com} 6, 32 -- Message-ID: {C3EA0A57.2BBE2%rui.vilar-at-ist.utl.pt} 6, 32 -- Thread-Topic: Electron microscopy position 6, 32 -- Thread-Index: Ach4pRu3WizCiuSYEdyQyQAX8gBXUA== 6, 32 -- Mime-version: 1.0 6, 32 -- Content-type: text/plain; 6, 32 -- charset="ISO-8859-1" 6, 32 -- Content-Transfer-Encoding: 8bit 6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1QIRLHh005897 ==============================End of - Headers==============================
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Title-Subject: [Filtered] exploding ceramics in FESEM
Question: I was just imaging a carbon coated piece of ceramic in our FESEM under normal conditions (dry, 0.8kV 5mm WD) and switched to a higher kV. Nothing I have not done before. Much to my surprise, the piece exploded, knocking three other samples off their respective stubs, and reducing the piece I was looking at to a pile of dust. This is a first for me. Has anyone experienced this before? Can anyone offer an explanation so I might avoid this in the future? Sorry, but I can't offer any more sample information at this time.
New York Microscopical Society 30 North Mountain Avenue Montclair, NJ 07042
Bernard Friedman Memorial Workshop
Polarized Light Microscopy May 3, 10, 17 & 24, 2008
An advanced course on polarized light microscopy which will cover the following topics: The nature of polarized light The origin and interpretation of interference colors Birefringence and crystal orientation, The Indicatrix Compensation and variable compensators Interference figures and their interpretation
The workshop will consist of four Consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of polarized light microscopy. The course instructors include Jan Hinsch formally of Leica, Inc., Mary McCann of McCann Imaging, John Reffner of Smiths Detection and N.Y.M.S. Instructor Don O'Leary.
WHEN: May 3, 10, 17 & 24, 2008 from 10 A.M. to 4 P.M.
COST: $425 for N.Y.M.S. members, $455 for non-members (includes membership). Lunch and course materials are included. Checks made out to N.Y.M.S.
WHO: advanced course for those who have completed "The Use of the Microscope" or are experienced in microscopy and familiar with the theory of its use.
HOW: Register using the form below. Limited to the first 12 registrants. Return form to Don O'Leary, 10 Sampson Street, Unit 113, Saddle Brook, NJ 07663 FURTHER INFORMATION: Call D. O'Leary (201)368-8849 e-mail dkoleary-at-verizon.net
PLEASE POST -------------------------------------------------------------------------- Registration Form Polarized Light Microscopy
Derrick, I've seen the mineral Lawsonite do this (very impressively) under the electron beam. I believe the term is "decrepitation".
This mineral contains a water molecule locked inside the crystal structure which might have something to do with it. john
At 10:50 AM 2/26/2008, dhorne-at-interchange.ubc.ca wrote:
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==============================Original Headers============================== 7, 24 -- From donovan-at-uoregon.edu Tue Feb 26 13:20:45 2008 7, 24 -- Received: from QMTA04.westchester.pa.mail.comcast.net (qmta04.westchester.pa.mail.comcast.net [76.96.62.40]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QJKjic013212 7, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 13:20:45 -0600 7, 24 -- Message-Id: {200802261920.m1QJKjic013212-at-ns.microscopy.com} 7, 24 -- Received: from OMTA12.westchester.pa.mail.comcast.net ([76.96.62.44]) 7, 24 -- by QMTA04.westchester.pa.mail.comcast.net with comcast 7, 24 -- id uCUh1Y0090xGWP8540T800; Tue, 26 Feb 2008 19:20:10 +0000 7, 24 -- Received: from SOURCE.uoregon.edu ([71.193.189.123]) 7, 24 -- by OMTA12.westchester.pa.mail.comcast.net with comcast 7, 24 -- id uKLj1Y0022gBFvr3Y00000; Tue, 26 Feb 2008 19:20:44 +0000 7, 24 -- X-Authority-Analysis: v=1.0 c=1 a=AIIq51hXrNMA:10 a=Zx37jsudAAAA:8 7, 24 -- a=HDmXKJO6idVEDcgGnKIA:9 a=j649Y4E1Vxf9r4jcc-IA:7 7, 24 -- a=6VYg4HFndyPmgAz0hRfcaBYwjDkA:4 a=G91thX30KR8A:10 a=9OHTkwyHC8cA:10 7, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 24 -- Date: Tue, 26 Feb 2008 11:18:41 -0800 7, 24 -- To: Microscopy-at-microscopy.com 7, 24 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 7, 24 -- Subject: Re: [Microscopy] viaWWW: exploding ceramics in FESEM 7, 24 -- Cc: dhorne-at-interchange.ubc.ca 7, 24 -- In-Reply-To: {200802261850.m1QIoDJP027309-at-ns.microscopy.com} 7, 24 -- References: {200802261850.m1QIoDJP027309-at-ns.microscopy.com} 7, 24 -- Mime-Version: 1.0 7, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I got a few answers from my request for class text recommendations. Several people also mentioned the plethora of websites with basic light microscopy information useful for introductory classes, so I've included a few of my bookmarks. It was nice to find the AFIP Methods manual still available, although Kiernan's text isn't. (Arg!) Foster's book "Optimizing the Light Microscope ..." is also out of print, although she still has a few copies left (there may be a new edition sometime, possibly). A major criterion was expense. There are lots of books available for $80 to $100 and more, a few of which cover both the basics of light microscopy and microscopes *and* microtechnique, but there are very few of these in the light microscopy realm. Unlike the EM world.
Phil P.S. Sorry Jim, I didn't find anything on embedding and microtomy or H&E staining in the Handbook. Or on Hoffman Modulation ...
Books:
Douglas Murphy; Fundamentals of Light Microscopy and Electronic Imaging put out by Wiley.
Basic Methods in Microscopy: Protocols And Concepts from Cells, a Laboratory Manual (Paperback) David L. Spector (Editor), Robert D. Goldman (Editor)
Introduction to Light Microscopy (Microscopy Handbooks) (Paperback) by Mrs H Bradbury Royal Microscopy Society
Contrast Techniques in Light Microscopy, by Bradbury & Bracegirdle 1996 Royal Microscopy Society Plus, several of the other books in the RMS series
Microscope series by Mortimer Abramowitz http://www.olympusamerica.com/seg_section/seg_emporiumbooks.asp
"Laboratory Methods in Histotechnology" E.B. Prophet et al. AFIP LABORATORY METH {=also known as FS07 $35.00 + $5.00 shp Prepayment is required on all orders. Fax your prepaid order to 1-301-578-1693. If you would rather pay by check please mail your order to: AMERICAN REGISTRY OF PATHOLOGY PUBLICATIONS DEPT. PO BOX 8188 SILVER SPRING, MD 20907 We accept VISA, Master Card and American Express. publications-at-arppress.org
Web sites: Nikon Microscopy U http://www.microscopyu.com/sitemap.html Olympus Microscopy Resource http://www.olympusmicro.com/ Molecular Expressions http://micro.magnet.fsu.edu/index.html (Molecular Expressions) Molecular Probes Fluorescence http://probes.invitrogen.com/resources/education/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 11, 20 -- From oshel1pe-at-cmich.edu Tue Feb 26 14:59:05 2008 11, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QKx5Xk029996 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 14:59:05 -0600 11, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 11, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m1QLEL9N002225 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 16:14:22 -0500 11, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 11, 20 -- Tue, 26 Feb 2008 15:58:49 -0500 11, 20 -- Mime-Version: 1.0 11, 20 -- Message-Id: {f06240808c3ea2cd9ed10-at-[141.209.160.249]} 11, 20 -- Date: Tue, 26 Feb 2008 15:59:00 -0500 11, 20 -- To: Microscopy-at-microscopy.com 11, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 11, 20 -- Subject: book & web site recommendations 11, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 20 -- X-OriginalArrivalTime: 26 Feb 2008 20:58:50.0185 (UTC) FILETIME=[63185B90:01C878BA] 11, 20 -- X-CanItPRO-Stream: default 11, 20 -- X-Spam-Score: -4 () L_EXCH_MF 11, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Hi all, For SEM gold-labeling studies, do most people use the DeHarven et al. (1984) method or the Helinski et al 1990 study to localize cell surface receptors using antibodies conjugated to gold?
Is there a new "gold" standard? Sorry couldn't resist the bad pun.
Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
*************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
==============================Original Headers============================== 13, 19 -- From beth-at-plantbio.uga.edu Tue Feb 26 15:13:01 2008 13, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QLD1Cr010582 13, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 15:13:01 -0600 13, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 13, 19 -- (authenticated user beth-at-plantbio.uga.edu) 13, 19 -- by dogwood.plantbio.uga.edu 13, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 13, 19 -- for microscopy-at-microscopy.com; 13, 19 -- Tue, 26 Feb 2008 16:12:56 -0500 13, 19 -- Message-Id: {AA5F2BBB-1F30-4245-855E-DCE2AAE4F973-at-plantbio.uga.edu} 13, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 13, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 19 -- Content-Transfer-Encoding: 7bit 13, 19 -- Mime-Version: 1.0 (Apple Message framework v919.2) 13, 19 -- Subject: SEM and gold-labeling 13, 19 -- Date: Tue, 26 Feb 2008 16:13:00 -0500 13, 19 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
The homogeneity of gold/silver particles is influenced by a range of factors. Size differences of the gold particles and the quality of silver enhancement are only one side to the story, but more importantly the specimen and the particular enhancement conditions play a role that should not be overlooked. And as a last factor, inhomogeneity may also be related to fusion of gold/silver particles during the enhancement process.
As far as the aspects of reagents and procedure are concerned, uniformity of the diameter of the gold/silver particles using Aurion R-Gent SE-EM may be improved by slowing down the formation process using a developer with an initiator:activator ratio of 1:60 (instead of the 1:40 ratio that is in general sufficient). This change needs to be compensated by an increase in enhancement time.
Next to the paper by Yi et al, we would like to cite the impressive paper by Ravi C. Balijepalli et al., which appeared in PNAS, May 9, 2006, vol 103(19), pp 7500-7505.
We hope these ideas help you further.
Jan Leunissen and Peter van de Plas
Aurion Costerweg 5 6702 AA Wageningen The Netherlands phone 31-317-497676 fax 31-317-415955 http://www.aurion.nl -----------------------------------------------------------------
==============================Original Headers============================== 14, 24 -- From leunissen-at-aurion.nl Tue Feb 26 15:20:30 2008 14, 24 -- Received: from mta02.xtra.co.nz (mta02.xtra.co.nz [210.54.141.253]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QLKSbm023344 14, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 15:20:29 -0600 14, 24 -- Received: from fep04.xtra.co.nz ([172.23.12.41]) by mta02.xtra.co.nz 14, 24 -- with ESMTP 14, 24 -- id {20080226212023.KORU7182.mta02.xtra.co.nz-at-fep04.xtra.co.nz} ; 14, 24 -- Wed, 27 Feb 2008 10:20:23 +1300 14, 24 -- Received: from [192.168.1.50] (really [122.57.249.42]) by fep04.xtra.co.nz 14, 24 -- with ESMTP 14, 24 -- id {20080226212022.DJSQ17371.fep04.xtra.co.nz-at-[192.168.1.50]} ; 14, 24 -- Wed, 27 Feb 2008 10:20:22 +1300 14, 24 -- In-Reply-To: {200802192256.m1JMuOAI009359-at-ns.microscopy.com} 14, 24 -- References: {200802192256.m1JMuOAI009359-at-ns.microscopy.com} 14, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 14, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 14, 24 -- Message-Id: {E6A4415B-C4DB-45EC-88DF-DE5A2604F412-at-aurion.nl} 14, 24 -- Cc: microscopy-at-microscopy.com 14, 24 -- Content-Transfer-Encoding: 7bit 14, 24 -- From: Jan Leunissen {leunissen-at-aurion.nl} 14, 24 -- Subject: Re: [Microscopy] viaWWW: Detergents for Pre-embedding Immunogold and More 14, 24 -- Date: Wed, 27 Feb 2008 10:25:54 +1300 14, 24 -- To: modla-at-dbi.udel.edu 14, 24 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
Zhang Z et al. (1999) Localization of myosin on sperm-cell-associated membranes of tobacco. Protoplasma 208:123-128
I am not sure if that a "gold" standard, but we did use SEM and 15 nm "gold" for the labeling. Let me know if you like to have a reprint.
Zhaojie
Zhaojie Zhang, Ph.D. Director, Microscopy Core Facility Department of Zoology and Physiology University of Wyoming Laramie, WY 82071 TEL: 307-766-3038 FAX: 307-766-5625 zzhang-at-uwyo.edu http://www.uwyo.edu/microscopy
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Tuesday, February 26, 2008 2:17 PM To: Z.J. Zhang
Hi all, For SEM gold-labeling studies, do most people use the DeHarven et al. (1984) method or the Helinski et al 1990 study to localize cell surface receptors using antibodies conjugated to gold?
Is there a new "gold" standard? Sorry couldn't resist the bad pun.
Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
************************************************************************ *** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
==============================Original Headers============================== 13, 19 -- From beth-at-plantbio.uga.edu Tue Feb 26 15:13:01 2008 13, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QLD1Cr010582 13, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 15:13:01 -0600 13, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 13, 19 -- (authenticated user beth-at-plantbio.uga.edu) 13, 19 -- by dogwood.plantbio.uga.edu 13, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 13, 19 -- for microscopy-at-microscopy.com; 13, 19 -- Tue, 26 Feb 2008 16:12:56 -0500 13, 19 -- Message-Id: {AA5F2BBB-1F30-4245-855E-DCE2AAE4F973-at-plantbio.uga.edu} 13, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 13, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 19 -- Content-Transfer-Encoding: 7bit 13, 19 -- Mime-Version: 1.0 (Apple Message framework v919.2) 13, 19 -- Subject: SEM and gold-labeling 13, 19 -- Date: Tue, 26 Feb 2008 16:13:00 -0500 13, 19 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
==============================Original Headers============================== 26, 30 -- From ZZhang-at-uwyo.edu Tue Feb 26 15:26:53 2008 26, 30 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QLQrYa003619 26, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 15:26:53 -0600 26, 30 -- Received: from UWMAIL.uwyo.edu (uwmail.uwyo.edu [172.26.4.76]) 26, 30 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id m1QLQi6Z019147; 26, 30 -- Tue, 26 Feb 2008 14:26:52 -0700 (MST) 26, 30 -- (envelope-from ZZhang-at-uwyo.edu) 26, 30 -- Received: from TELEGRAPH5.uwyo.edu ([10.84.60.120]) by UWMAIL.uwyo.edu with Microsoft SMTPSVC(6.0.3790.3959); 26, 30 -- Tue, 26 Feb 2008 14:26:47 -0700 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 30 -- Content-class: urn:content-classes:message 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Type: text/plain; 26, 30 -- charset="us-ascii" 26, 30 -- Subject: RE: [Microscopy] SEM and gold-labeling 26, 30 -- Date: Tue, 26 Feb 2008 14:26:38 -0700 26, 30 -- Message-ID: {C9C1AF307F12AF4087DEF87CB0E6A4AD02042E70-at-TELEGRAPH5.uwyo.edu} 26, 30 -- In-Reply-To: {200802262116.m1QLGpQR017573-at-ns.microscopy.com} 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- Thread-Topic: [Microscopy] SEM and gold-labeling 26, 30 -- Thread-Index: Ach4vO93OGmPYwlyQGCrUWuyFUTm2AAAKDvw 26, 30 -- References: {200802262116.m1QLGpQR017573-at-ns.microscopy.com} 26, 30 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 26, 30 -- To: {beth-at-plantbio.uga.edu} 26, 30 -- Cc: {Microscopy-at-microscopy.com} 26, 30 -- X-OriginalArrivalTime: 26 Feb 2008 21:26:47.0854 (UTC) FILETIME=[4B1054E0:01C878BE] 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1QLQrYa003619 ==============================End of - Headers==============================
I recommend that you check out Amazon.com for scientific texts. I have found several important texts at Amazon (through the variety of booksellers they use) that are out of print. The prices can be remarkably low, i.e. used copies of a non-scientific book I was looking for ranged in price from $1.03 to $16.00.
Regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
oshel1pe-at-cmich .edu To gary.m.brown-at-exxonmobil.com 02/26/08 03:01 cc PM Subject [Microscopy] book & web site Please respond recommendations to oshel1pe-at-cmich .edu
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Listers,
I got a few answers from my request for class text recommendations. Several people also mentioned the plethora of websites with basic light microscopy information useful for introductory classes, so I've included a few of my bookmarks. It was nice to find the AFIP Methods manual still available, although Kiernan's text isn't. (Arg!) Foster's book "Optimizing the Light Microscope ..." is also out of print, although she still has a few copies left (there may be a new edition sometime, possibly). A major criterion was expense. There are lots of books available for $80 to $100 and more, a few of which cover both the basics of light microscopy and microscopes *and* microtechnique, but there are very few of these in the light microscopy realm. Unlike the EM world.
Phil P.S. Sorry Jim, I didn't find anything on embedding and microtomy or H&E staining in the Handbook. Or on Hoffman Modulation ...
Books:
Douglas Murphy; Fundamentals of Light Microscopy and Electronic Imaging put out by Wiley.
Basic Methods in Microscopy: Protocols And Concepts from Cells, a Laboratory Manual (Paperback) David L. Spector (Editor), Robert D. Goldman (Editor)
Introduction to Light Microscopy (Microscopy Handbooks) (Paperback) by Mrs H Bradbury Royal Microscopy Society
Contrast Techniques in Light Microscopy, by Bradbury & Bracegirdle 1996 Royal Microscopy Society Plus, several of the other books in the RMS series
Microscope series by Mortimer Abramowitz http://www.olympusamerica.com/seg_section/seg_emporiumbooks.asp
"Laboratory Methods in Histotechnology" E.B. Prophet et al. AFIP LABORATORY METH {=also known as FS07 $35.00 + $5.00 shp Prepayment is required on all orders. Fax your prepaid order to 1-301-578-1693. If you would rather pay by check please mail your order to: AMERICAN REGISTRY OF PATHOLOGY PUBLICATIONS DEPT. PO BOX 8188 SILVER SPRING, MD 20907 We accept VISA, Master Card and American Express. publications-at-arppress.org
Web sites: Nikon Microscopy U http://www.microscopyu.com/sitemap.html Olympus Microscopy Resource http://www.olympusmicro.com/ Molecular Expressions http://micro.magnet.fsu.edu/index.html (Molecular Expressions) Molecular Probes Fluorescence http://probes.invitrogen.com/resources/education/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 11, 20 -- From oshel1pe-at-cmich.edu Tue Feb 26 14:59:05 2008 11, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QKx5Xk029996 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 14:59:05 -0600 11, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 11, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m1QLEL9N002225 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 16:14:22 -0500 11, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 11, 20 -- Tue, 26 Feb 2008 15:58:49 -0500 11, 20 -- Mime-Version: 1.0 11, 20 -- Message-Id: {f06240808c3ea2cd9ed10-at-[141.209.160.249]} 11, 20 -- Date: Tue, 26 Feb 2008 15:59:00 -0500 11, 20 -- To: Microscopy-at-microscopy.com 11, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 11, 20 -- Subject: book & web site recommendations 11, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 20 -- X-OriginalArrivalTime: 26 Feb 2008 20:58:50.0185 (UTC) FILETIME=[63185B90:01C878BA] 11, 20 -- X-CanItPRO-Stream: default 11, 20 -- X-Spam-Score: -4 () L_EXCH_MF 11, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 19 -- From gary.m.brown-at-exxonmobil.com Tue Feb 26 16:54:32 2008 34, 19 -- Received: from dalspc02.exxonmobil.com (dalspc02.exxonmobil.com [131.126.223.2]) 34, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QMsVlN018722 34, 19 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 16:54:31 -0600 34, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 34, 19 -- by dalspc02.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m1QMsTwa005284; 34, 19 -- Tue, 26 Feb 2008 16:54:29 -0600 (CST) 34, 19 -- In-Reply-To: {200802262101.m1QL1Sr6000707-at-ns.microscopy.com} 34, 19 -- Subject: Re: [Microscopy] book & web site recommendations 34, 19 -- Importance: 34, 19 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com 34, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 34, 19 -- Message-ID: {OF6CB488E1.506F8F4A-ON862573FB.007D48EE-862573FB.007DD526-at-exxonmobil.com} 34, 19 -- From: gary.m.brown-at-exxonmobil.com 34, 19 -- Date: Tue, 26 Feb 2008 16:54:25 -0600 34, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 34, 19 -- 02, 2006) at 02/26/2008 04:54:29 PM 34, 19 -- MIME-Version: 1.0 34, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nicastro-at-brandeis.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nicastro-at-brandeis.edu Name: Dr. Daniela Nicastro
Organization: Brandeis University, MA, USA
Title-Subject: [Filtered] Postdoc & EM Technician Positions available
Question: Dear All, 5 positions are available immediately in the lab of Dr. Daniela Nicastro at Brandeis University, MA, USA (near Boston).
1) We have openings for two candidates (one Postdoc and one EM Technician) with experience in high-pressure freezing, freeze-substitution and ultramicrotomy. The successful candidates will join the newly implemented cellular visualization facility for correlative light and electron microscopy to work closely with cell and neurobiologists. Further details about this opportunity are available at: {www.bio.brandeis.edu/nicastrolab/CLEM-Postdoc2008.pdf} {www.bio.brandeis.edu/nicastrolab/EM-Technician2008.pdf}
2) A postdoctoral position is available to study the 3D structure and function of molecular motors using cryo-electron tomography (Nicastro et al. 2006 Science 313:944). Further details about this opportunity are available at: {www.bio.brandeis.edu/nicastrolab/Motor_Postdoc2008.pdf}
3) Another postdoctoral position to study cilia and flagella in the model organism Chlamydomonas or Tetrahymena, using molecular genetic approaches including RNAi technology, biochemistry and high-resolution imaging. Further details about this opportunity are available at: {www.bio.brandeis.edu/nicastrolab/Flagella_Postdoc2008.pdf}
4) And a postdoctoral position to study the proteomics of cytoskeletal structures using biochemical approaches, mass spectrometry and imaging. Further details about this opportunity are available at: {www.bio.brandeis.edu/nicastrolab/Biochemistry_Postdoc2008.pdf}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hgabrisc-at-uno.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hgabrisc-at-uno.edu Name: Heike Gabrisch
Organization: University of New Orleans
Title-Subject: [Filtered] Post doctoral position
Question: Post doctoral position available at the University of New Orleans in a project on the development of new materials for thermoelectric applications. The responsibilities of this position focus on microstructural characterization of Half-Heusler based alloys with nano sized inclusions by TEM. Contact : Heike Gabrisch, hgabrisc-at-uno.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dgmorgan-at-ucdavis.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dgmorgan-at-ucdavis.edu Name: David Morgan
Organization: University of California
Title-Subject: [Filtered] book & web site recommendations
Question: Another great place to pick up out of print science books is through http://www.alibris.com. I haven't looked for any microscopy books there, but I have found a number of volumes dealing with crystallography that have been impossible to find elsewhere.
You can also search for a paper by Sarah Ellis. Sarah presented her beautiful pre-embedding double labeling work at the Ft. Lauderdale MSA meeting last August. I had the impression that she was planning to put out a paper soon after that. Thank you.
Hong
On Feb 26, 2008, at 4:22 PM, leunissen-at-aurion.nl wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Shannon, } } The homogeneity of gold/silver particles is influenced by a range of } factors. Size differences of the gold particles and the quality of } silver enhancement are only one side to the story, but more } importantly the specimen and the particular enhancement conditions } play a role that should not be overlooked. And as a last factor, } inhomogeneity may also be related to fusion of gold/silver particles } during the enhancement process. } } As far as the aspects of reagents and procedure are concerned, } uniformity of the diameter of the gold/silver particles using Aurion } R-Gent SE-EM may be improved by slowing down the formation process } using a developer with an initiator:activator ratio of 1:60 (instead } of the 1:40 ratio that is in general sufficient). } This change needs to be compensated by an increase in enhancement } time. } } Next to the paper by Yi et al, we would like to cite the impressive } paper by Ravi C. Balijepalli et al., which appeared in PNAS, May 9, } 2006, vol 103(19), pp 7500-7505. } } We hope these ideas help you further. } } } Jan Leunissen and Peter van de Plas } } Aurion } Costerweg 5 } 6702 AA Wageningen } The Netherlands } phone 31-317-497676 } fax 31-317-415955 } http://www.aurion.nl } ----------------------------------------------------------------- } } } } } } } } ==============================Original } Headers============================== } 14, 24 -- From leunissen-at-aurion.nl Tue Feb 26 15:20:30 2008 } 14, 24 -- Received: from mta02.xtra.co.nz (mta02.xtra.co.nz } [210.54.141.253]) } 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m1QLKSbm023344 } 14, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 } 15:20:29 -0600 } 14, 24 -- Received: from fep04.xtra.co.nz ([172.23.12.41]) by } mta02.xtra.co.nz } 14, 24 -- with ESMTP } 14, 24 -- id } {20080226212023.KORU7182.mta02.xtra.co.nz-at-fep04.xtra.co.nz} ; } 14, 24 -- Wed, 27 Feb 2008 10:20:23 +1300 } 14, 24 -- Received: from [192.168.1.50] (really [122.57.249.42]) by } fep04.xtra.co.nz } 14, 24 -- with ESMTP } 14, 24 -- id {20080226212022.DJSQ17371.fep04.xtra.co.nz-at- } [192.168.1.50]} ; } 14, 24 -- Wed, 27 Feb 2008 10:20:22 +1300 } 14, 24 -- In-Reply-To: {200802192256.m1JMuOAI009359-at-ns.microscopy.com} } 14, 24 -- References: {200802192256.m1JMuOAI009359-at-ns.microscopy.com} } 14, 24 -- Mime-Version: 1.0 (Apple Message framework v753) } 14, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 14, 24 -- Message-Id: {E6A4415B-C4DB-45EC-88DF-DE5A2604F412-at-aurion.nl} } 14, 24 -- Cc: microscopy-at-microscopy.com } 14, 24 -- Content-Transfer-Encoding: 7bit } 14, 24 -- From: Jan Leunissen {leunissen-at-aurion.nl} } 14, 24 -- Subject: Re: [Microscopy] viaWWW: Detergents for Pre- } embedding Immunogold and More } 14, 24 -- Date: Wed, 27 Feb 2008 10:25:54 +1300 } 14, 24 -- To: modla-at-dbi.udel.edu } 14, 24 -- X-Mailer: Apple Mail (2.753) } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 5, 24 -- From hyi-at-emory.edu Tue Feb 26 21:23:56 2008 5, 24 -- Received: from QMTA08.westchester.pa.mail.comcast.net (qmta08.westchester.pa.mail.comcast.net [76.96.62.80]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1R3Nu5L012171 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 21:23:56 -0600 5, 24 -- Received: from OMTA11.westchester.pa.mail.comcast.net ([76.96.62.36]) 5, 24 -- by QMTA08.westchester.pa.mail.comcast.net with comcast 5, 24 -- id uDzF1Y00E0mv7h0580zx00; Wed, 27 Feb 2008 03:23:30 +0000 5, 24 -- Received: from [71.236.25.99] ([71.236.25.99]) 5, 24 -- by OMTA11.westchester.pa.mail.comcast.net with comcast 5, 24 -- id uTPw1Y00C28HRMB3X00000; Wed, 27 Feb 2008 03:23:56 +0000 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=dq7uUA0Yzp8A:10 a=Zx37jsudAAAA:8 5, 24 -- a=DAAJhXmmAAAA:8 a=Y_leFtGsTWkjgpmjkFMA:9 a=U7SVkjGsluBgkqzLI5sA:7 5, 24 -- a=00Tfv21NRKnluT43gNIw4Mr2lT8A:4 a=G91thX30KR8A:10 a=7mayv77iRtYA:10 5, 24 -- Mime-Version: 1.0 (Apple Message framework v752.3) 5, 24 -- In-Reply-To: {200802262122.m1QLMewm027660-at-ns.microscopy.com} 5, 24 -- References: {200802262122.m1QLMewm027660-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 24 -- Message-Id: {52DA387C-1120-4D9E-BACB-9935139CEB4F-at-emory.edu} 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- From: Hong Yi {hyi-at-emory.edu} 5, 24 -- Subject: Re: [Microscopy] Re: viaWWW: Detergents for Pre-embedding Immunogold and More 5, 24 -- Date: Tue, 26 Feb 2008 22:23:55 -0500 5, 24 -- To: microscopy-at-microscopy.com 5, 24 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
I had too such "explosions" of samples. Once it was with an organic cristal, containing much OH, which slowly dehydrated under the vacuum and craked. It was too carbon coated, and suddendly it exploded trought the charge accumation in the splits.
An other time it was with a very bad sol-gel ceramic, where the grains were bad sintered. Charge accumulation dissociated the grains. One could see grains flying away individually until the sample intself exploded.
In the first case, the deshydratation giving cracks, which disrupted the carbon film, and charge could accumulate between ungrounded parts, which push back themself. In the second case, the carbon coting, as it is a point source evaporation, doesn't give a continous conductive film, between the grains. Same effect than with the former. And as grains fly away, the effect became more and more dramatic !
The ways to avoid this would be : - what you have done, low voltage SEM, at the "right" primary energy (not always easy to find, and changing from one place to an other), - If the sample is porous, multiple carbon coatings, with the sample tilted in different orientation in respect to the source. Or use magnetron Ir, Cr, Os coating, which is less directive. - low-vac / esem, where the charge cancelation is better done than in high vac SEMs. But, this is not compatible with FE-SEM for high resolution. It depends of the magnification you need to see what you want.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
dhorne-at-interchange.ubc.ca a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both dhorne-at-interchange.ubc.ca as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: dhorne-at-interchange.ubc.ca } Name: Derrick Horne } } Organization: Univeristy of British Columbia } } Title-Subject: [Filtered] exploding ceramics in FESEM } } Question: I was just imaging a carbon coated piece of ceramic in our FESEM under normal conditions (dry, 0.8kV 5mm WD) and switched to a higher kV. Nothing I have not done before. Much to my surprise, the piece exploded, knocking three other samples off their respective stubs, and reducing the piece I was looking at to a pile of dust. This is a first for me. Has anyone experienced this before? Can anyone offer an explanation so I might avoid this in the future? Sorry, but I can't offer any more sample information at this time. } } Login Host: 137.82.85.210 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Tue Feb 26 12:45:01 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QIixc2018879 } 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 12:45:00 -0600 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240803c3ea0e8ed2b8-at-[206.69.208.22]} } 6, 11 -- Date: Tue, 26 Feb 2008 12:44:57 -0600 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: dhorne-at-interchange.ubc.ca (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: exploding ceramics in FESEM } 6, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 11, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Feb 27 01:48:19 2008 11, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1R7mHUI032601 11, 29 -- for {microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 01:48:18 -0600 11, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 11, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m1R7mGb7025298 11, 29 -- for {microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 08:48:16 +0100 (CET) 11, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id C6F945E4004 11, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 27 Feb 2008 08:47:07 +0100 (CET) 11, 29 -- Message-ID: {47C51589.5010807-at-ipcms.u-strasbg.fr} 11, 29 -- Date: Wed, 27 Feb 2008 08:47:21 +0100 11, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 11, 29 -- MIME-Version: 1.0 11, 29 -- To: Microscopy-at-microscopy.com 11, 29 -- Subject: Re: [Microscopy] viaWWW: exploding ceramics in FESEM 11, 29 -- References: {200802261850.m1QIoGFh027428-at-ns.microscopy.com} 11, 29 -- In-Reply-To: {200802261850.m1QIoGFh027428-at-ns.microscopy.com} 11, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-IPCMS-MailScanner: Found to be clean 11, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 11, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.154]); Wed, 27 Feb 2008 08:48:16 +0100 (CET) 11, 29 -- X-Virus-Scanned: ClamAV 0.92.1/6006/Wed Feb 27 02:03:40 2008 on mr4.u-strasbg.fr 11, 29 -- X-Virus-Status: Clean 11, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=disabled 11, 29 -- version=3.2.4 11, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr4.u-strasbg.fr ==============================End of - Headers==============================
Scientific Services at The Jackson Laboratory is conducting bench-marking surveys in an effort to develop an understanding of the best current practices offered by peer institutions. In order to do this, we have developed a short survey, which will not be posted on this listserver, in accordance with the regulations. It is NOT a salary survey. Insteads, it deals with staffing, work volume, types of services offered to facility users and the cost of those services. If you are a core facility manager who is interested in this data and would be willing to fill out a short survey, please email me directly and I will forward the survey link to you. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution.
In advance, thank you very much.
Lesley Bechtold
Lesley S. Bechtold Senior Manager, Histopathology & Microscopy The Jackson Laboratory 600 Main St. Bar Harbor ME 04609 207-288-6322
==============================Original Headers============================== 7, 22 -- From lesley.bechtold-at-jax.org Wed Feb 27 07:36:53 2008 7, 22 -- Received: from ms1.jax.org (ms1.jax.org [192.43.249.188]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1RDar1I026494 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 07:36:53 -0600 7, 22 -- Received: from ms1.jax.org (localhost [127.0.0.1]) 7, 22 -- by localhost (Postfix) with SMTP id 67BC231831 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 08:36:53 -0500 (EST) 7, 22 -- Received: from jcs-mid-prod.jax.org (jcs-mid-prod.jax.org [192.43.249.134]) 7, 22 -- by ms1.jax.org (Postfix) with ESMTP id 1742B31808 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 08:36:53 -0500 (EST) 7, 22 -- Received: from jcs-mid-prod by jcs-mid-prod.jax.org 7, 22 -- with ESMTP id 292262641204119383; Wed, 27 Feb 2008 08:36:23 -0500 7, 22 -- Message-ID: {23478459.1204119383528.JavaMail.ocsadmin-at-jcs-mid-prod.jax.org} 7, 22 -- Date: Wed, 27 Feb 2008 08:36:23 -0500 (EST) 7, 22 -- From: lesley.bechtold-at-jax.org 7, 22 -- To: microscopy-at-microscopy.com 7, 22 -- Subject: Seeking Core EM Facility Information 7, 22 -- Mime-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-Priority: 3 7, 22 -- X-Mailer: Oracle Webmail Client(UIX) ==============================End of - Headers==============================
I would like to thank both Jan and Hong for their advice and comments. Our lab uses Aurion's products on a regular basis and we were mainly interested in troubleshooting our problems and optimizing our techniques. As we have never tried to perform a double-labeling silver enhancement experiment in the past, we did not anticipate it to work perfectly the first time, and many variables still need to be worked out. I greatly appreciate all the responses.
Sincerely,
Shannon
==============================Original Headers============================== 3, 24 -- From modla-at-dbi.udel.edu Wed Feb 27 08:42:20 2008 3, 24 -- Received: from mail.dbi.udel.edu (genome3.dbi.udel.edu [128.175.253.195]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1REgKPp009303 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 08:42:20 -0600 3, 24 -- Received: from localhost (localhost [127.0.0.1]) 3, 24 -- by mail.dbi.udel.edu (Postfix) with ESMTP id 2E311193ED8 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 09:42:20 -0500 (EST) 3, 24 -- X-Virus-Scanned: amavisd-new at dbi.udel.edu 3, 24 -- Received: from mail.dbi.udel.edu ([127.0.0.1]) 3, 24 -- by localhost (mail.dbi.udel.edu [127.0.0.1]) (amavisd-new, port 10024) 3, 24 -- with ESMTP id dZCraumK7AjL for {Microscopy-at-microscopy.com} ; 3, 24 -- Wed, 27 Feb 2008 09:42:19 -0500 (EST) 3, 24 -- Received: from [128.175.253.84] (host-253-84.nss.udel.edu [128.175.253.84]) 3, 24 -- by mail.dbi.udel.edu (Postfix) with ESMTP id 99830193ED6 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 09:42:19 -0500 (EST) 3, 24 -- Message-ID: {47C576CD.1060100-at-dbi.udel.edu} 3, 24 -- Date: Wed, 27 Feb 2008 09:42:21 -0500 3, 24 -- From: Shannon Modla {modla-at-dbi.udel.edu} 3, 24 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 3, 24 -- MIME-Version: 1.0 3, 24 -- To: Microscopy-at-microscopy.com 3, 24 -- Subject: Detergents for Pre-embedding Immunogold and More 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A colleague of a colleague is in need of advice on how to image ostracodes under the fluorescent microscope. If anyone has experience, feel free to email directly.
Thanks,
Scott Whittaker Head NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
-----Original Message----- X-from: Kornicker, Louis Sent: Wednesday, February 27, 2008 8:33 AM To: Whittaker, Scott
Philip,
I recommend that you check out Amazon.com for scientific texts. I have found several important texts at Amazon (through the variety of booksellers they use) that are out of print. The prices can be remarkably low, i.e. used copies of a non-scientific book I was looking for ranged in price from $1.03 to $16.00.
Regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
oshel1pe-at-cmich .edu To gary.m.brown-at-exxonmobil.com 02/26/08 03:01 cc PM Subject [Microscopy] book & web site Please respond recommendations to oshel1pe-at-cmich .edu
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Listers,
I got a few answers from my request for class text recommendations. Several people also mentioned the plethora of websites with basic light microscopy information useful for introductory classes, so I've included a few of my bookmarks. It was nice to find the AFIP Methods manual still available, although Kiernan's text isn't. (Arg!) Foster's book "Optimizing the Light Microscope ..." is also out of print, although she still has a few copies left (there may be a new edition sometime, possibly). A major criterion was expense. There are lots of books available for $80 to $100 and more, a few of which cover both the basics of light microscopy and microscopes *and* microtechnique, but there are very few of these in the light microscopy realm. Unlike the EM world.
Phil P.S. Sorry Jim, I didn't find anything on embedding and microtomy or H&E staining in the Handbook. Or on Hoffman Modulation ...
Books:
Douglas Murphy; Fundamentals of Light Microscopy and Electronic Imaging put out by Wiley.
Basic Methods in Microscopy: Protocols And Concepts from Cells, a Laboratory Manual (Paperback) David L. Spector (Editor), Robert D. Goldman (Editor)
Introduction to Light Microscopy (Microscopy Handbooks) (Paperback) by Mrs H Bradbury Royal Microscopy Society
Contrast Techniques in Light Microscopy, by Bradbury & Bracegirdle 1996 Royal Microscopy Society Plus, several of the other books in the RMS series
Microscope series by Mortimer Abramowitz http://www.olympusamerica.com/seg_section/seg_emporiumbooks.asp
"Laboratory Methods in Histotechnology" E.B. Prophet et al. AFIP LABORATORY METH {=also known as FS07 $35.00 + $5.00 shp Prepayment is required on all orders. Fax your prepaid order to 1-301-578-1693. If you would rather pay by check please mail your order to: AMERICAN REGISTRY OF PATHOLOGY PUBLICATIONS DEPT. PO BOX 8188 SILVER SPRING, MD 20907 We accept VISA, Master Card and American Express. publications-at-arppress.org
Web sites: Nikon Microscopy U http://www.microscopyu.com/sitemap.html Olympus Microscopy Resource http://www.olympusmicro.com/ Molecular Expressions http://micro.magnet.fsu.edu/index.html (Molecular Expressions) Molecular Probes Fluorescence http://probes.invitrogen.com/resources/education/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 11, 20 -- From oshel1pe-at-cmich.edu Tue Feb 26 14:59:05 2008 11, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1QKx5Xk029996 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 14:59:05 -0600 11, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 11, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m1QLEL9N002225 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Feb 2008 16:14:22 -0500 11, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 11, 20 -- Tue, 26 Feb 2008 15:58:49 -0500 11, 20 -- Mime-Version: 1.0 11, 20 -- Message-Id: {f06240808c3ea2cd9ed10-at-[141.209.160.249]} 11, 20 -- Date: Tue, 26 Feb 2008 15:59:00 -0500 11, 20 -- To: Microscopy-at-microscopy.com 11, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 11, 20 -- Subject: book & web site recommendations 11, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 20 -- X-OriginalArrivalTime: 26 Feb 2008 20:58:50.0185 (UTC) FILETIME=[63185B90:01C878BA] 11, 20 -- X-CanItPRO-Stream: default 11, 20 -- X-Spam-Score: -4 () L_EXCH_MF 11, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 19 -- From gary.m.brown-at-exxonmobil.com Wed Feb 27 10:38:15 2008 34, 19 -- Received: from dalspc02.exxonmobil.com (dalspc02.exxonmobil.com [131.126.223.2]) 34, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1RGcEsV011974 34, 19 -- for {microscopy-at-microscopy.com} ; Wed, 27 Feb 2008 10:38:14 -0600 34, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 34, 19 -- by dalspc02.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m1RGc8l5026255; 34, 19 -- Wed, 27 Feb 2008 10:38:09 -0600 (CST) 34, 19 -- In-Reply-To: {200802262101.m1QL1Sr6000707-at-ns.microscopy.com} 34, 19 -- Subject: Re: [Microscopy] book & web site recommendations 34, 19 -- Importance: 34, 19 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com 34, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 34, 19 -- Message-ID: {OF2F9D03F0.A62C18C7-ON862573FC.005B3BAE-862573FC.005B6185-at-exxonmobil.com} 34, 19 -- From: gary.m.brown-at-exxonmobil.com 34, 19 -- Date: Wed, 27 Feb 2008 10:38:07 -0600 34, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 34, 19 -- 02, 2006) at 02/27/2008 10:38:09 AM 34, 19 -- MIME-Version: 1.0 34, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
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Email: gnf3-at-cdc.gov Name: Maureen Metcalfe
Title-Subject: [Filtered] Carl Zeiss LSM 510
Question: Hi All, I am looking for a software and operating manual for a Carl Zeiss LSM 510 confocal. Does anyone have one they do not need any longer? Please contact me offline. Thank you, Maureen Metcalfe
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Email: friess-at-limedion.de Name: Frank Friess
Organization: LMI
Title-Subject: [Filtered] Used Emitter for JEOL 6xxxF
Question: Hello,
is there anyone here who maybe has an out of order cold field emitter of a 6300F, 6400F or 6600F and doesn¥t need it anymore ?
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Email: robert.snyder-at-fei.com Name: Robert W. Snyder
Organization: Max Planck Institute of Biochemistry & FEI Company
Title-Subject: [Filtered] Frontiers in Microscopy Symposium
Question: Conference: Frontiers in Microscopy Symposium
Frontiers in Microscopy Symposium plans to discuss and highlight future perspectives for the development and application of imaging techniques in structural biology and the impact for providing a molecular and structural understanding of fundamental processes in biology, medicine and human health.
More information: http://www.biochem.mpg.de/KRIOSymposium/
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A friend just sent me this note - I thought it was news worthy;-)
The GWB Library
The George W. Bush Presidential Library is now in the planning stages. You'll want to be the first at your corporation to make a contribution to this great man's legacy. The Library will include: The Hurricane Katrina Room, which is still under construction. The Alberto Gonzales Room, where you can't remember anything. The Texas Air National Guard Room, where you don't have to even show up.The Walter Reed Hospital Room, where they don't let you in. The Guantanamo Bay Room, where they don't let you out. The Weapons of Mass Destruction Room (which no one has been able to find).The Iraq War Room.After you complete your first tour, they make you to go back for a second, third, fourth, and sometimes fifth tour. The Dick Cheney Room, in the famous undisclosed location, complete with shooting gallery. Plans also include: The K- Street Project Gift Shop - where you can buy (or just steal) an election. The Airport Men's Room, where you can meet some of your favorite Republican Senators. Last, but not least, there will be an entire floor devoted to a 7/8 scale model of the President's ego. To highlight the President's accomplishments, the museum will have an electron microscope to help you locate them. When asked, President Bush said that he didn't care so much about the individual exhibits as long as his museum was better than his father's.
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
*************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
==============================Original Headers============================== 16, 19 -- From beth-at-plantbio.uga.edu Thu Feb 28 16:10:19 2008 16, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1SMAI9w027043 16, 19 -- for {microscopy-at-microscopy.com} ; Thu, 28 Feb 2008 16:10:19 -0600 16, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 16, 19 -- (authenticated user beth-at-plantbio.uga.edu) 16, 19 -- by dogwood.plantbio.uga.edu 16, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 16, 19 -- for microscopy-at-microscopy.com; 16, 19 -- Thu, 28 Feb 2008 17:10:12 -0500 16, 19 -- Message-Id: {C4D904E9-B175-47F2-AAF4-E16CD18A4090-at-plantbio.uga.edu} 16, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 16, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 16, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 16, 19 -- Content-Transfer-Encoding: 7bit 16, 19 -- Mime-Version: 1.0 (Apple Message framework v919.2) 16, 19 -- Subject: Electron Microscope in the Bush Library 16, 19 -- Date: Thu, 28 Feb 2008 17:10:16 -0500 16, 19 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
"I vaguely remembered a publication on this subject where the author(s) mentioned the size of the epitope, the dimensions and shape of primary and secondary antibodies (IgG) and the gold particles that seem to explain the localization of gold particles to the side of the epitope (structure) and may not be over the epitope. Can you give me the citation? Any other souces would be great."
Wen-Lang Lin, pH.D=20 Mayo Clinic=20 4500 San Pablo Road=20 Jacksonville, FL 32224=20
==============================Original Headers============================== 5, 22 -- From PhillipsT-at-missouri.edu Fri Feb 29 06:49:38 2008 5, 22 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TCnc5s029038 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 06:49:38 -0600 5, 22 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 22 -- Fri, 29 Feb 2008 06:49:38 -0600 5, 22 -- x-mimeole: Produced By Microsoft Exchange V6.5 5, 22 -- Content-class: urn:content-classes:message 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; 5, 22 -- charset="US-ASCII" 5, 22 -- Subject: 5, 22 -- Date: Fri, 29 Feb 2008 06:49:37 -0600 5, 22 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD03AF11A2-at-UM-XMAIL06.um.umsystem.edu} 5, 22 -- X-MS-Has-Attach: 5, 22 -- X-MS-TNEF-Correlator: 5, 22 -- Thread-Index: Ach60YryiWygkzZiRZ+I8/7m3iD0og== 5, 22 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- X-OriginalArrivalTime: 29 Feb 2008 12:49:38.0358 (UTC) FILETIME=[8B483D60:01C87AD1] 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1TCnc5s029038 ==============================End of - Headers==============================
Dear Listers Does anyone out there have experience of retipping filaments? We get ours done by Joel and they charge quite a lot for what would seem to be a simple process (with a high degree of duds as well). I just wanted know if this was something we could do with our facilities or would require some special technique/kit that is hard to do in a standard EM lab. Any suggestions about how to 'DIY' would be gratefully received. Regards John Bristol University, UK
==============================Original Headers============================== 1, 27 -- From john.mitchels-at-gmail.com Fri Feb 29 08:57:54 2008 1, 27 -- Received: from wr-out-0506.google.com (wr-out-0506.google.com [64.233.184.228]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TEvrdN019867 1, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 08:57:54 -0600 1, 27 -- Received: by wr-out-0506.google.com with SMTP id c57so7136786wra.4 1, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 06:57:53 -0800 (PST) 1, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 27 -- d=gmail.com; s=gamma; 1, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- bh=alJz4jcEG1FpeqkMFPku8s6fkFVUB8RnS+4rcF4/VE0=; 1, 27 -- b=JUCeEg9v7WaNoc3kx00FU6qUCCJsIh6TwpEezRC0BhMG8oPIkwrBtKc/QDTJP8wdGcMbUAX44p6M/HcAvvcvYB8pLXefNyflct43CAm0Mkz+L8H5izkvP99RJUk9UTfXWQsLLgnbh1ivPRM433t+m78RtPRB2LgTJBrus6mUSpw= 1, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 1, 27 -- d=gmail.com; s=gamma; 1, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 1, 27 -- b=v4HXGobXkiqQEi2LnbSA5yY/H7whZgynmiaQHypUMiTO6DdZj8z1C9S/O5IESeksv4O6mgeidxoOH6yk1lNkgWb/wz1yWSzV74tynLghmGVUL5GSfRZCMxygLP3W+BLgGDSqOJDZJliQXEGN7LDFbJ8WIKYQf5rCz6qXJ0mKTw0= 1, 27 -- Received: by 10.141.35.21 with SMTP id n21mr6398372rvj.115.1204297071951; 1, 27 -- Fri, 29 Feb 2008 06:57:51 -0800 (PST) 1, 27 -- Received: by 10.141.33.4 with HTTP; Fri, 29 Feb 2008 06:57:51 -0800 (PST) 1, 27 -- Message-ID: {1b3cf7c30802290657q6f7a1091r147ba206a803323c-at-mail.gmail.com} 1, 27 -- Date: Fri, 29 Feb 2008 14:57:51 +0000 1, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 1, 27 -- To: Microscopy-at-microscopy.com 1, 27 -- Subject: Filament Retipping 1, 27 -- MIME-Version: 1.0 1, 27 -- Content-Type: text/plain; charset=ISO-8859-1 1, 27 -- Content-Transfer-Encoding: 7bit 1, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I always appreciate a humor in all its forms, however, let me remind you that the Listserver rules state explicitly that off-topic (i.e. non-microscopy/microanalysis) subjects belong elsewhere. This most definitely includes political humor.
Please respect your colleagues and keep to our theme. If you are ever unsure, feel free to send me the posting first for comment. I tend to lean on the side of approval of most postings as long as I can see a benefit to the overall community.
You can also refer to the FAQ page for the general rules of operaton at:
Simmons, S.R. and R.M. Albrecht. 1989. Probe size and bound label conformation in colloidal gold-ligand labels and gold-immunolabels. Scanning Microscopy Suppl. 3:27-34.
Daryl Meyer
} "I vaguely remembered a publication on this subject where the author(s) } mentioned the size of the epitope, the dimensions and shape of primary } and secondary antibodies (IgG) and the gold particles that seem to } explain the localization of gold particles to the side of the epitope } (structure) and may not be over the epitope. Can you give me the } citation? Any other souces would be great." } } Wen-Lang Lin, pH.D=20 } Mayo Clinic=20 } 4500 San Pablo Road=20 } Jacksonville, FL 32224=20
==============================Original Headers============================== 5, 33 -- From dameyer-at-wisc.edu Fri Feb 29 09:18:16 2008 5, 33 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TFIGPY013783 5, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:18:16 -0600 5, 33 -- MIME-version: 1.0 5, 33 -- Content-transfer-encoding: 7BIT 5, 33 -- Content-disposition: inline 5, 33 -- Content-type: text/plain; charset=us-ascii 5, 33 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 5, 33 -- smtpauth1.wiscmail.wisc.edu 5, 33 -- (Sun Java(tm) System Messaging Server 6.3-5.02 (built Oct 12 2007; 32bit)) 5, 33 -- id {0JX000600AHSXB00-at-smtpauth1.wiscmail.wisc.edu} for 5, 33 -- Microscopy-at-microscopy.com; Fri, 29 Feb 2008 09:17:52 -0600 (CST) 5, 33 -- Received: from wiscmail.wisc.edu (store1.doit.wisc.edu [144.92.8.164]) 5, 33 -- by smtpauth1.wiscmail.wisc.edu 5, 33 -- (Sun Java(tm) System Messaging Server 6.3-5.02 (built Oct 12 2007; 32bit)) 5, 33 -- with ESMTP id {0JX000245AHSK820-at-smtpauth1.wiscmail.wisc.edu} for 5, 33 -- Microscopy-at-microscopy.com; Fri, 29 Feb 2008 09:17:52 -0600 (CST) 5, 33 -- Received: from [144.92.8.150] (Forwarded-For: 144.92.132.175, [127.0.0.1]) 5, 33 -- by store1.doit.wisc.edu (mshttpd); Fri, 29 Feb 2008 09:17:51 -0600 5, 33 -- Date: Fri, 29 Feb 2008 09:17:51 -0600 5, 33 -- From: Daryl Meyer {dameyer-at-wisc.edu} 5, 33 -- Subject: Re: [Microscopy] 5, 33 -- To: Microscopy-at-microscopy.com 5, 33 -- Message-id: {e48ea6377d44.47c7cdbf-at-wiscmail.wisc.edu} 5, 33 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-8.04 (built Feb 28 2007) 5, 33 -- Content-language: en 5, 33 -- X-Accept-Language: en 5, 33 -- Priority: normal 5, 33 -- X-Spam-Report: TrustedSender=yes, SenderIP=144.92.8.164 5, 33 -- X-Spam-PmxInfo: Server=avs-6, Version=5.4.1.325704, 5, 33 -- Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.2.29.70313, 5, 33 -- SenderIP=144.92.8.164 ==============================End of - Headers==============================
It has been a few years (someone phasing out a scope gave me several boxes of K-type filaments) but I had some JEOL filaments re-worked by Energy Beam Sciences and they seemed very good. I tried both standard and their AR filaments and they were well formed and centered as well as new JEOL filaments, and they worked well. I think they would do a few for you to see their product. Link below.
Dale Callaham University of Massachusetts -at- Amherst
john.mitchels-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } Does anyone out there have experience of retipping filaments? We get } ours done by Joel and they charge quite a lot for what would seem to } be a simple process (with a high degree of duds as well). I just } wanted know if this was something we could do with our facilities or } would require some special technique/kit that is hard to do in a } standard EM lab. Any suggestions about how to 'DIY' would be } gratefully received. } Regards } John } Bristol University, UK } } ==============================Original Headers============================== } 1, 27 -- From john.mitchels-at-gmail.com Fri Feb 29 08:57:54 2008 } 1, 27 -- Received: from wr-out-0506.google.com (wr-out-0506.google.com [64.233.184.228]) } 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TEvrdN019867 } 1, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 08:57:54 -0600 } 1, 27 -- Received: by wr-out-0506.google.com with SMTP id c57so7136786wra.4 } 1, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 06:57:53 -0800 (PST) } 1, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 1, 27 -- d=gmail.com; s=gamma; } 1, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 1, 27 -- bh=alJz4jcEG1FpeqkMFPku8s6fkFVUB8RnS+4rcF4/VE0=; } 1, 27 -- b=JUCeEg9v7WaNoc3kx00FU6qUCCJsIh6TwpEezRC0BhMG8oPIkwrBtKc/QDTJP8wdGcMbUAX44p6M/HcAvvcvYB8pLXefNyflct43CAm0Mkz+L8H5izkvP99RJUk9UTfXWQsLLgnbh1ivPRM433t+m78RtPRB2LgTJBrus6mUSpw= } 1, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 1, 27 -- d=gmail.com; s=gamma; } 1, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 1, 27 -- b=v4HXGobXkiqQEi2LnbSA5yY/H7whZgynmiaQHypUMiTO6DdZj8z1C9S/O5IESeksv4O6mgeidxoOH6yk1lNkgWb/wz1yWSzV74tynLghmGVUL5GSfRZCMxygLP3W+BLgGDSqOJDZJliQXEGN7LDFbJ8WIKYQf5rCz6qXJ0mKTw0= } 1, 27 -- Received: by 10.141.35.21 with SMTP id n21mr6398372rvj.115.1204297071951; } 1, 27 -- Fri, 29 Feb 2008 06:57:51 -0800 (PST) } 1, 27 -- Received: by 10.141.33.4 with HTTP; Fri, 29 Feb 2008 06:57:51 -0800 (PST) } 1, 27 -- Message-ID: {1b3cf7c30802290657q6f7a1091r147ba206a803323c-at-mail.gmail.com} } 1, 27 -- Date: Fri, 29 Feb 2008 14:57:51 +0000 } 1, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} } 1, 27 -- To: Microscopy-at-microscopy.com } 1, 27 -- Subject: Filament Retipping } 1, 27 -- MIME-Version: 1.0 } 1, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 1, 27 -- Content-Transfer-Encoding: 7bit } 1, 27 -- Content-Disposition: inline } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 22 -- From dac-at-research.umass.edu Fri Feb 29 09:32:26 2008 6, 22 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TFWQWB027038 6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:32:26 -0600 6, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 22 -- (authenticated bits=0) 6, 22 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1TFWPDv021974 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 10:32:25 -0500 6, 22 -- Message-ID: {47C8266D.9050603-at-research.umass.edu} 6, 22 -- Date: Fri, 29 Feb 2008 10:36:13 -0500 6, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 22 -- Reply-To: dac-at-research.umass.edu 6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 6, 22 -- MIME-Version: 1.0 6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] Filament Retipping 6, 22 -- References: {200802291508.m1TF85vC005493-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {200802291508.m1TF85vC005493-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Thank you all for your responses, I'm afraid I have worded my email rather ambiguously I meant to say that I was interested to know how easy it is to re tip the filaments myself rather than getting an external company to do it. I just wondered how it was done and whether anyone had experience of the process themselves in their own lab.
Thanks again John
==============================Original Headers============================== 3, 27 -- From john.mitchels-at-gmail.com Fri Feb 29 09:55:12 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TFtBvg008165 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:55:12 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so3650736rvb.30 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 07:55:10 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=Uz9LQuOI1uYAKLHS6tv0pyqk7Z6N41djFJ+bGwrsmbI=; 3, 27 -- b=lrsJdyesmgy+hTyLqECtUyOdaNN2KYEoVWz972rYXZ9pXa05bMgQ40i6d1DE57w+FTSKKueWc5LwBUSKVCHex1WrYGHkx83UYOPHOYwaArzfFVoYaXZGlNuY6p7FNWTa2xoYpxs/V8H+Oi0t3pRw4NIq/VjlRzMIAC+NcIR+Hos= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=vS05uBnfimwIpmf2GY4mmEppAt27IhnrP6yHFWaseM3Onq6KXyLWGDfiks9pfC+TRBT23lyMZMGz9E1SOHBJWUEd3X1m96KT/MH64KVKuu9F0xbDoYDfhVKtYmg9a+8Lpuyb/ghDUTNThhieQ28YYOrbPuSpGYPt7YMJSjFfjns= 3, 27 -- Received: by 10.141.1.2 with SMTP id d2mr6468194rvi.42.1204300510353; 3, 27 -- Fri, 29 Feb 2008 07:55:10 -0800 (PST) 3, 27 -- Received: by 10.141.33.4 with HTTP; Fri, 29 Feb 2008 07:55:10 -0800 (PST) 3, 27 -- Message-ID: {1b3cf7c30802290755t2637d96bgaef8295c74077323-at-mail.gmail.com} 3, 27 -- Date: Fri, 29 Feb 2008 15:55:10 +0000 3, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- Subject: Retipped Filaments part 2 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Dear John, Many years ago I used to re-tip the filaments of a Hitachi HU11-A TEM. These were simple, bent-wire filaments, not the twisted type. I bent a piece of 10-thou tungsten wire over a razor blade, cut to size and spot-welded the wire onto the posts of an old filament base. The TEM had a wide range of movement in the gun, so the centering was not too critical. The modern instruments often do not have enough range of gun alignment to use anything but their own, carefully pre-centered filaments. Perhaps if you could build a jig to hold everything in exactly the right place for the spot-welding, that would help. When I looked into re-tipping my modern Hitachi filaments, the cost of getting them re-tipped by an EM supplier was no lower than buying new, and they are too precise to do myself. Good luck.
-----Original Message----- X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] Sent: February 29, 2008 8:04 AM To: maryflet-at-interchange.ubc.ca
Dear Listers
Thank you all for your responses, I'm afraid I have worded my email rather ambiguously I meant to say that I was interested to know how easy it is to re tip the filaments myself rather than getting an external company to do it. I just wondered how it was done and whether anyone had experience of the process themselves in their own lab.
Thanks again John
==============================Original Headers============================== 3, 27 -- From john.mitchels-at-gmail.com Fri Feb 29 09:55:12 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TFtBvg008165 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:55:12 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so3650736rvb.30 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 07:55:10 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=Uz9LQuOI1uYAKLHS6tv0pyqk7Z6N41djFJ+bGwrsmbI=; 3, 27 -- b=lrsJdyesmgy+hTyLqECtUyOdaNN2KYEoVWz972rYXZ9pXa05bMgQ40i6d1DE57w+FTSKKueWc5 LwBUSKVCHex1WrYGHkx83UYOPHOYwaArzfFVoYaXZGlNuY6p7FNWTa2xoYpxs/V8H+Oi0t3pRw4N Iq/VjlRzMIAC+NcIR+Hos= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer -encoding:content-disposition; 3, 27 -- b=vS05uBnfimwIpmf2GY4mmEppAt27IhnrP6yHFWaseM3Onq6KXyLWGDfiks9pfC+TRBT23lyMZM Gz9E1SOHBJWUEd3X1m96KT/MH64KVKuu9F0xbDoYDfhVKtYmg9a+8Lpuyb/ghDUTNThhieQ28YYO rbPuSpGYPt7YMJSjFfjns= 3, 27 -- Received: by 10.141.1.2 with SMTP id d2mr6468194rvi.42.1204300510353; 3, 27 -- Fri, 29 Feb 2008 07:55:10 -0800 (PST) 3, 27 -- Received: by 10.141.33.4 with HTTP; Fri, 29 Feb 2008 07:55:10 -0800 (PST) 3, 27 -- Message-ID: {1b3cf7c30802290755t2637d96bgaef8295c74077323-at-mail.gmail.com} 3, 27 -- Date: Fri, 29 Feb 2008 15:55:10 +0000 3, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- Subject: Retipped Filaments part 2 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 12, 35 -- From maryflet-at-interchange.ubc.ca Fri Feb 29 11:48:21 2008 12, 35 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 12, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1THmL3l025584 12, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 11:48:21 -0600 12, 35 -- X-Ubc-Received: from mr6.mail-relay.ubc.ca (localhost [127.0.0.1]) 12, 35 -- by localhost (Postfix) with SMTP id CE3A815C61 12, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:48:20 -0800 (PST) 12, 35 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 12, 35 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP 12, 35 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:48:18 -0800 (PST) 12, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 12, 35 -- by smtp.interchange.ubc.ca 12, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 12, 35 -- with ESMTPS id {0JX000J09HGI3T-at-smtp.interchange.ubc.ca} for 12, 35 -- microscopy-at-microscopy.com; Fri, 29 Feb 2008 09:48:18 -0800 (PST) 12, 35 -- Date: Fri, 29 Feb 2008 09:48:01 -0800 12, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 12, 35 -- Subject: RE: [Microscopy] Retipped Filaments part 2 12, 35 -- In-reply-to: {200802291603.m1TG3XZ9018742-at-ns.microscopy.com} 12, 35 -- To: john.mitchels-at-gmail.com 12, 35 -- Cc: microscopy-at-microscopy.com 12, 35 -- Reply-to: maryflet-at-interchange.ubc.ca 12, 35 -- Message-id: {0JX000J0AHGI3T-at-smtp.interchange.ubc.ca} 12, 35 -- Organization: Materials Eng. 12, 35 -- MIME-version: 1.0 12, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 12, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 12, 35 -- Content-type: text/plain; charset=us-ascii 12, 35 -- Content-transfer-encoding: 7bit 12, 35 -- Thread-index: Ach67KNCDfGADr4CRAareHccqpTbPwACD8Mw 12, 35 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.2.29.93056 12, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 12, 35 -- X-PerlMx-Spam: Probability=7%, Report=ECARD_KNOWN_DOMAINS 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0 12, 35 -- X-Spam-Level: 12, 35 -- X-Spam-Flag: No ==============================End of - Headers==============================
I have a colleague who would like to get some SEM photos of the underside of a mushroom that grows locally on the sides of trees down here in Florida. I was wondering what experiences people have had drying such a specimen. Air drying will surely distort the structure, but I don't have access to a critical point dryer.
Any suggestions?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 27 -- From kraftpiano-at-gmail.com Fri Feb 29 17:18:50 2008 4, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TNIneU022162 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 17:18:50 -0600 4, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so3849233rvb.30 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 15:18:49 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=1zqEw4BiVmrMYEL/2xlrxwnyi0KOpEE34Rwo3jviKEg=; 4, 27 -- b=Ufa/+VVs+sYPFmOfb1pb46DWIpFowtDKKxg18xSJ0mKY++8Mi6AMJhA0Dh9rxWvIy+YynYENK1pxE7PISXZMalHJPhXCnCiCiFCSlh8CRqyAqa1Ho/me6jLsROR461EWTmjemxKjAP2FVtP5XoriqHe5O8k3f9V2oir/4tYQhzo= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=BAiyeR/Leqy30bDbbfPVbGwtUx/vhtyCQ8feOkm0klhn7hqOMDQFIE5qbgKADCXYGWkOiSuYKtWUY2VfwMYPrg7qr3eAaAIN1zklGxT7WFdnosn4c6rJjctc1ppCcNu+dvQM6dGL9EDhrYgIZkvgtuLOZA51T1A5zDgWp+q32rU= 4, 27 -- Received: by 10.141.28.12 with SMTP id f12mr6799656rvj.1.1204327129541; 4, 27 -- Fri, 29 Feb 2008 15:18:49 -0800 (PST) 4, 27 -- Received: by 10.140.161.11 with HTTP; Fri, 29 Feb 2008 15:18:49 -0800 (PST) 4, 27 -- Message-ID: {25e2b0d20802291518h3855dd6atafb352170574c9f5-at-mail.gmail.com} 4, 27 -- Date: Fri, 29 Feb 2008 18:18:49 -0500 4, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: SEM Specimen prep: This week, Fungus! 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I'm not a mycologist. Don't let the fungus mature too far, or dry out or it may shed the spores. If it is important, keep some sample fixed and dehydrated to 70% ethanol (store in fridge or freezer) and maybe you can get access to a CPD unit later.
There is a method for drying using hexamethyldisilazane (HMDS). This compound is available from the usual EM supply houses. This has a much lower surface tension than water and will probably do much better. You fix with aldehyde as usual, and postfix and dehydrate to 100% ethanol, immerse in HMDS for 5 min (small samples, longer for larger pieces), then drain and air-dry. There may be some special methods mycologists use. May be a good idea to en bloc treat with 2% aq UAc to add conductivity, or use one of the osmium-thiocarbohydrazide-osmium treatments.
Be careful with the HMDS, it is unstable when mixed with ethanol, yet that is how is is used. Read the MSDS well. I had a bottle of waste that developed much pressure after sitting a while.
There is another method for ebedding in a wax (Peldri, maybe from Ted Pella?) that sublimes under vacuum, but I've never used it. I read that it is a mess for the pump, etc., but gentler.
Hope this helps,
Dale
kraftpiano-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have a colleague who would like to get some SEM photos of the } underside of a mushroom that grows locally on the sides of trees down } here in Florida. I was wondering what experiences people have had } drying such a specimen. Air drying will surely distort the structure, } but I don't have access to a critical point dryer. } } Any suggestions? } } --Justin A. Kraft }
==============================Original Headers============================== 10, 20 -- From dac-at-research.umass.edu Fri Feb 29 17:53:37 2008 10, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TNrbJm003051 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 17:53:37 -0600 10, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 10, 20 -- (authenticated bits=0) 10, 20 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m1TNralo011255 10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 18:53:36 -0500 10, 20 -- Message-ID: {47C89B18.3080307-at-research.umass.edu} 10, 20 -- Date: Fri, 29 Feb 2008 18:54:00 -0500 10, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 10, 20 -- MIME-Version: 1.0 10, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 10, 20 -- Subject: Re: [Microscopy] SEM Specimen prep: This week, Fungus! 10, 20 -- References: {200802292325.m1TNPmc3031049-at-ns.microscopy.com} 10, 20 -- In-Reply-To: {200802292325.m1TNPmc3031049-at-ns.microscopy.com} 10, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
In my experience, most fungal tissues do not do well in HMDS. Likewise plant tissues are all over the map- some work well, others not so.
Vapor fixation over osmium crystal a couple of days in the fridge followed by slow air or freeze drying would be my first plan of attack, though admittadly I have not prepped the cap before.
________________________________
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Fri 2/29/2008 6:20 PM To: Whittaker, Scott
I have a colleague who would like to get some SEM photos of the underside of a mushroom that grows locally on the sides of trees down here in Florida. I was wondering what experiences people have had drying such a specimen. Air drying will surely distort the structure, but I don't have access to a critical point dryer.
Any suggestions?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 27 -- From kraftpiano-at-gmail.com Fri Feb 29 17:18:50 2008 4, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TNIneU022162 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 17:18:50 -0600 4, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so3849233rvb.30 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 15:18:49 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=1zqEw4BiVmrMYEL/2xlrxwnyi0KOpEE34Rwo3jviKEg=; 4, 27 -- b=Ufa/+VVs+sYPFmOfb1pb46DWIpFowtDKKxg18xSJ0mKY++8Mi6AMJhA0Dh9rxWvIy+YynYENK1pxE7PISXZMalHJPhXCnCiCiFCSlh8CRqyAqa1Ho/me6jLsROR461EWTmjemxKjAP2FVtP5XoriqHe5O8k3f9V2oir/4tYQhzo= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=BAiyeR/Leqy30bDbbfPVbGwtUx/vhtyCQ8feOkm0klhn7hqOMDQFIE5qbgKADCXYGWkOiSuYKtWUY2VfwMYPrg7qr3eAaAIN1zklGxT7WFdnosn4c6rJjctc1ppCcNu+dvQM6dGL9EDhrYgIZkvgtuLOZA51T1A5zDgWp+q32rU= 4, 27 -- Received: by 10.141.28.12 with SMTP id f12mr6799656rvj.1.1204327129541; 4, 27 -- Fri, 29 Feb 2008 15:18:49 -0800 (PST) 4, 27 -- Received: by 10.140.161.11 with HTTP; Fri, 29 Feb 2008 15:18:49 -0800 (PST) 4, 27 -- Message-ID: {25e2b0d20802291518h3855dd6atafb352170574c9f5-at-mail.gmail.com} 4, 27 -- Date: Fri, 29 Feb 2008 18:18:49 -0500 4, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: SEM Specimen prep: This week, Fungus! 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 17, 28 -- From WHITTAKS-at-si.edu Sat Mar 1 08:54:25 2008 17, 28 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m21EsOrX019743 17, 28 -- for {Microscopy-at-msa.microscopy.com} ; Sat, 1 Mar 2008 08:54:24 -0600 17, 28 -- Received: from SI-MSESMTPO-02.US.SINET.SI.EDU (SI-MSESMTP-N2.us.sinet.si.edu [160.111.49.76]) 17, 28 -- by si-mailout03.si.edu (Postfix) with ESMTP id 918226B1E; 17, 28 -- Sat, 1 Mar 2008 09:54:24 -0500 (EST) 17, 28 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.71]) by SI-MSESMTPO-02.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959); 17, 28 -- Sat, 1 Mar 2008 09:54:24 -0500 17, 28 -- x-mimeole: Produced By Microsoft Exchange V6.5 17, 28 -- Content-class: urn:content-classes:message 17, 28 -- MIME-Version: 1.0 17, 28 -- Content-Type: text/plain; 17, 28 -- charset="iso-8859-1" 17, 28 -- Subject: RE: [Microscopy] SEM Specimen prep: This week, Fungus! 17, 28 -- Date: Sat, 1 Mar 2008 09:54:23 -0500 17, 28 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA30133593A-at-SI-ECL02.US.SINET.SI.EDU} 17, 28 -- X-MS-Has-Attach: 17, 28 -- X-MS-TNEF-Correlator: 17, 28 -- Thread-Topic: [Microscopy] SEM Specimen prep: This week, Fungus! 17, 28 -- Thread-Index: Ach7KbWI58zyT+8uSmCLlaHs+kzlcgAgO9L/ 17, 28 -- References: {200802292320.m1TNKfYf024655-at-ns.microscopy.com} 17, 28 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 17, 28 -- To: {kraftpiano-at-gmail.com} 17, 28 -- Cc: "microscopy-at-msa.microscopy.com" {Microscopy-at-msa.microscopy.com} 17, 28 -- X-OriginalArrivalTime: 01 Mar 2008 14:54:24.0382 (UTC) FILETIME=[23B699E0:01C87BAC] 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m21EsOrX019743 ==============================End of - Headers==============================
You won't get good results if you don't CPD. I suggest you fix the specimens in glutar or PAF and keep them until you find CPD. If you wish send the vials to me and I will prepare them for you, for free.
This project is intended to support the continued functionality of older "Ultracut" (AO-Ultracut through Ultracut-E) microtomes. The design and quality of these instruments is exceptional. The mechanical components can last a very long time with good care. However, factory support of the earlier models has been dropped by the current owners of the Reichert line (Leica) so the models up through the Ultracut-E no longer have factory support. There are many older Ultracuts out there and this is an effort to help them live on.
The "Open_Cut Microtome Project" will act as a hub for information-sharing in support of these older Ultracut models, and specifically for an "open source" project to discuss, develop and make available replacement electronics modules to support the aftermarket maintenance and continued life of these older Ultracuts. The goal is to develop a standardized approach to modifications that will be in the best interest of long-term continued support.
I have created a Google Group - initially set as private - this could be changed - I'm new to the "Groups" thing - "private" keeps the group member list accessible to group members only. You just need to email me to be "invited". It is fine to join and be passive; it will keep you connected to the project developments and not take your time. Participation is good too. The group has no formal rules, just civility. Anyone have other ideas?
My reason for doing this is that my facility at Umass, Amherst has several older Ultracuts, and one of them has a dead controller. I also work with electronics a bit, and I respect the older machines and would not want to see any of them abandoned and discarded. Please spread the word that the older microtomes are still of great value! I just wanted to get this started and get the word out, and would be happy to step back if someone else wants to take charge of the group. I don't mean to impose standards - they should come from consensus. But I have been doing some thinking and have some ideas and proposals to get the conversation flowing - see the project home page (link below). Please contact me if you are interested in any aspect of this project, but we should move most of the specific traffic off the Microscopy List. You are welcome to contact me personally, or via the Group email.
Since the group is designated as private, a search of Google will not show it. You will only see the group information if you join. Therefore I have also set up a web page to act as a public face for project information for those looking for information or not wanting to join a "group": Open_Cut Microtome Project (http://people.umass.edu/dac/Open_Cut/) with links for my personal email and group email. I will post summary "group" news on the web page from time to time.
Please bear with me since I don't have a firm grip on managing the "group" thing yet, not even sure I did it correctly! I'm a group of 1!
Aftermarket vendors who service older Ultracuts are encouraged to submit their contact information for listing to the Open_Cut group and the webpage; please include any info like region covered, etc..
Finally, I know it is unbelievable, but I think there are a couple of people not involved with the Microscopy Listserver, so if you know someone with an older Ultracut, they might find this information useful to tape to the back of their microtome.....
Thanks!
Dale Callaham
==============================Original Headers============================== 14, 18 -- From dac-at-research.umass.edu Sun Mar 2 14:18:21 2008 14, 18 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 14, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m22KIKLX020495 14, 18 -- for {microscopy-at-microscopy.com} ; Sun, 2 Mar 2008 14:18:21 -0600 14, 18 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 14, 18 -- (authenticated bits=0) 14, 18 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m22KIJdT027939 14, 18 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 14, 18 -- for {microscopy-at-microscopy.com} ; Sun, 2 Mar 2008 15:18:20 -0500 14, 18 -- Message-ID: {47CB0BA4.2000203-at-research.umass.edu} 14, 18 -- Date: Sun, 02 Mar 2008 15:18:44 -0500 14, 18 -- From: Dale Callaham {dac-at-research.umass.edu} 14, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 14, 18 -- MIME-Version: 1.0 14, 18 -- To: Microscopy List {microscopy-at-microscopy.com} 14, 18 -- Subject: Announcement: Group for those concerned with older Ultracut microtomes 14, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We recently aquired by donation an old (but for us new) Gatan slow scan CCD camera, model 694. We have almost all the pieces, but we are missing the cable between the data aquisition card in the Macintosh and the camera control unit.
It has DB37 male and female connectors.
Could anybody who is using this type of camera measure the cable and send me the pinout and connections? It would be great!
Thanks in advance!
Jakab-Farkas Laszlo
==============================Original Headers============================== 7, 29 -- From jakabfarkaslaszlo-at-gmail.com Sun Mar 2 14:56:08 2008 7, 29 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.184]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m22Ku8oi002278 7, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 2 Mar 2008 14:56:08 -0600 7, 29 -- Received: by rv-out-0910.google.com with SMTP id k20so5049189rvb.30 7, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 02 Mar 2008 12:56:08 -0800 (PST) 7, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 29 -- d=gmail.com; s=gamma; 7, 29 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 7, 29 -- bh=5meX+A6mXWPjlr6HVR4A3OM/CFXxOpd3wsB6VT7Gx0Q=; 7, 29 -- b=TlDjMVlCtpbm+/ZCxjwaaAJmcx+1stBBsCbot0Zm9PjenoMx3I7yaH30x5ZQ/OgHo/v5o5+LqA0FkylJvmoaSxZS4tfPWFDxIU9Jkds4mDg2xz5FzNK7CEnRiSSjHuP+m1AbExGjsfZJI+wHVDPyYZNApIiEcgjcsEXDxPpreyM= 7, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 29 -- d=gmail.com; s=gamma; 7, 29 -- h=message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 7, 29 -- b=XkA3cQRII3XKAUi07zqec2WNG+1FCBHSJ3TZwlkEfHGqiNyWhQ4lA/yWxVab2/Q00/9DhaQpOE2/XkuYv55oriPHYBSfecYjQtRXyLeg4nH2UQ4LYkt+yup63DXP0jyBoJWs/gi5TomZhspLfXU5HTLAWTFPG18Fm6q0aOMS7E8= 7, 29 -- Received: by 10.141.70.18 with SMTP id x18mr7481220rvk.284.1204491368086; 7, 29 -- Sun, 02 Mar 2008 12:56:08 -0800 (PST) 7, 29 -- Received: by 10.141.88.8 with HTTP; Sun, 2 Mar 2008 12:56:08 -0800 (PST) 7, 29 -- Message-ID: {a3da032b0803021256u79748bddof9c7c549826ad423-at-mail.gmail.com} 7, 29 -- Date: Sun, 2 Mar 2008 22:56:08 +0200 7, 29 -- From: "=?ISO-8859-1?Q?Laszl=F3_Jakab-Farkas?=" {jflaci-at-ms.sapientia.ro} 7, 29 -- Sender: jakabfarkaslaszlo-at-gmail.com 7, 29 -- To: Microscopy-at-microscopy.com 7, 29 -- Subject: Missing cable, could somebody help? 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; charset=ISO-8859-1 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- Content-Disposition: inline 7, 29 -- X-Google-Sender-Auth: f76f9eec1c441c84 ==============================End of - Headers==============================
One such paper is Drenkhahn and Dermietzel 1988 Journal of Cell Biology 107:1037, but there are others as well.
On 1/3/08 12:00 AM, "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } "I vaguely remembered a publication on this subject where the author(s) } mentioned the size of the epitope, the dimensions and shape of primary } and secondary antibodies (IgG) and the gold particles that seem to } explain the localization of gold particles to the side of the epitope } (structure) and may not be over the epitope. Can you give me the } citation? Any other souces would be great." } } Wen-Lang Lin, pH.D=20 } Mayo Clinic=20 } 4500 San Pablo Road=20 } Jacksonville, FL 32224=20 } } } } } ==============================Original Headers============================== } 5, 22 -- From PhillipsT-at-missouri.edu Fri Feb 29 06:49:38 2008 } 5, 22 -- Received: from um-tsmtpout1.um.umsystem.edu } (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m1TCnc5s029038 } 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 06:49:38 -0600 } 5, 22 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by } um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 5, 22 -- Fri, 29 Feb 2008 06:49:38 -0600 } 5, 22 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 5, 22 -- Content-class: urn:content-classes:message } 5, 22 -- MIME-Version: 1.0 } 5, 22 -- Content-Type: text/plain; } 5, 22 -- charset="US-ASCII" } 5, 22 -- Subject: } 5, 22 -- Date: Fri, 29 Feb 2008 06:49:37 -0600 } 5, 22 -- Message-ID: } {0510DC719E56F64BB2AD84EE64CE6BAD03AF11A2-at-UM-XMAIL06.um.umsystem.edu} } 5, 22 -- X-MS-Has-Attach: } 5, 22 -- X-MS-TNEF-Correlator: } 5, 22 -- Thread-Index: Ach60YryiWygkzZiRZ+I8/7m3iD0og== } 5, 22 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 5, 22 -- To: {microscopy-at-microscopy.com} } 5, 22 -- X-OriginalArrivalTime: 29 Feb 2008 12:49:38.0358 (UTC) } FILETIME=[8B483D60:01C87AD1] } 5, 22 -- Content-Transfer-Encoding: 8bit } 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m1TCnc5s029038 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 22 -- From prvs=Rosemary.White=940da5579-at-csiro.au Sun Mar 2 15:45:40 2008 6, 22 -- Received: from act-MTAout2.csiro.au (act-MTAout2.csiro.au [150.229.7.38]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m22Ljdvj016157 6, 22 -- for {microscopy-at-microscopy.com} ; Sun, 2 Mar 2008 15:45:39 -0600 6, 22 -- X-IronPort-AV: E=Sophos;i="4.25,435,1199624400"; 6, 22 -- d="scan'208";a="187314428" 6, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 6, 22 -- by act-ironport-int.csiro.au with ESMTP; 03 Mar 2008 08:45:25 +1100 6, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 6, 22 -- Mon, 3 Mar 2008 08:45:24 +1100 6, 22 -- User-Agent: Microsoft-Entourage/11.0.0.040405 6, 22 -- Date: Mon, 03 Mar 2008 08:50:15 +1100 6, 22 -- Subject: Re: [Microscopy] 6, 22 -- From: Rosemary White {rosemary.white-at-csiro.au} 6, 22 -- To: {microscopy-at-microscopy.com} 6, 22 -- Message-ID: {C3F16C47.9807%rosemary.white-at-csiro.au} 6, 22 -- In-Reply-To: {200802291300.m1TD0QLl007315-at-ns.microscopy.com} 6, 22 -- Mime-version: 1.0 6, 22 -- Content-type: text/plain; 6, 22 -- charset="US-ASCII" 6, 22 -- Content-transfer-encoding: 7bit 6, 22 -- X-OriginalArrivalTime: 02 Mar 2008 21:45:25.0181 (UTC) FILETIME=[B91BA6D0:01C87CAE] ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (jlbrennan-at-comcast.net) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, March 2, 2008 at 16:12:57 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jlbrennan-at-comcast.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: My lab book poses a few questions on heat fixing slide to which I can not find the answers anywhere. I have reviewed the lab book cover to cover, check the related text and have spent the last hour+ doing various Google searchs and the closes I have come to an answer is your site.
We have been making slides to Gram stain in class. The book asks why they have to air dry and why the process can not be sped up by gently heating them in a flame. Can you explain why this can not be done?
Second, can the slide be over heated during the fixing process? If so what happens?
Sorry about that bad web link. The correct link is: http://people.umass.edu/dac/projects/Open_Cut/
Dale
dac-at-research.umass.edu wrote:
} Since the group is designated as private, a search of Google will not } show it. You will only see the group information if you join. Therefore } I have also set up a web page to act as a public face for project } information for those looking for information or not wanting to join a } "group": Open_Cut Microtome Project (http://people.umass.edu/dac/Open_Cut/) } with links for my personal email and group email. I will post summary } "group" news on the web page from time to time. } } Please bear with me since I don't have a firm grip on managing the } "group" thing yet, not even sure I did it correctly! I'm a group of 1! }
==============================Original Headers============================== 4, 20 -- From dac-at-research.umass.edu Sun Mar 2 16:52:29 2008 4, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m22MqSVU010733 4, 20 -- for {microscopy-at-microscopy.com} ; Sun, 2 Mar 2008 16:52:28 -0600 4, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 4, 20 -- (authenticated bits=0) 4, 20 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m22MqRbs004663 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 20 -- for {microscopy-at-microscopy.com} ; Sun, 2 Mar 2008 17:52:28 -0500 4, 20 -- Message-ID: {47CB2FC5.8060506-at-research.umass.edu} 4, 20 -- Date: Sun, 02 Mar 2008 17:52:53 -0500 4, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 4, 20 -- MIME-Version: 1.0 4, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 4, 20 -- Subject: Re: [Microscopy] Announcement: Ultracut Group 4, 20 -- References: {200803022028.m22KSXdm029535-at-ns.microscopy.com} 4, 20 -- In-Reply-To: {200803022028.m22KSXdm029535-at-ns.microscopy.com} 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Recently, we have bought a new 200kV TEM with a 2k CCD. When we acquire an image in full frame mode, we observe that five pixels at the very ends of the CCD are merged into one. This repeats through-out the circumference of the CCD. So, my image has 2038x2038 pixels of useful data. In half and quarter mode acquisitions we do not see this problem.
Previously, I was using 1k CCD's and never experienced anything like this. Is there some kind of technical issue with 2k cameras that they have to give-up five pixels around the CCD?
Thank you, Ayten Celik-Aktas.
==============================Original Headers============================== 4, 27 -- From celikaktas-at-gmail.com Mon Mar 3 02:03:23 2008 4, 27 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.187]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2383MYb005883 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 02:03:22 -0600 4, 27 -- Received: by fk-out-0910.google.com with SMTP id 18so9865770fkq.2 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 03 Mar 2008 00:03:22 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=0OK6Lg6XvMPppGZ5udtOJnHtfiP/v1IhqEdz2uSjjuo=; 4, 27 -- b=wz+a8DcxRcFgDOkVs7HEUNMMj/VAHuvoIHzusip+ktyVvrAPKY+GZFp8UTaP8DF6LahOtcAwArXYb2rVZB9vLMpM0yGQlxzrZ9QuQB0iOfEoHL/LOGHhp69KyUS+y+QkB3c40rsmpSFVxE8+SRo/miWnORJcqLJzxlZq3Hv4bS8= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=BXFTzFmuUQMFvb3Bm589n41umq9tOFthu9Z/eHmLtN5Zy0smy7AsWBaLuOZn/YvnyJ+aKdxaq2H/Aorn0hReoE+Ti+jH37SUsZaN0PR8hYj6ROduZMLggI7My0yVMOM9Xb5db+UhzQPLvtSF+FRHSpW+YfekTCsKdIqLMyl/MVQ= 4, 27 -- Received: by 10.82.161.19 with SMTP id j19mr32121808bue.0.1204531401893; 4, 27 -- Mon, 03 Mar 2008 00:03:21 -0800 (PST) 4, 27 -- Received: by 10.82.125.5 with HTTP; Mon, 3 Mar 2008 00:03:21 -0800 (PST) 4, 27 -- Message-ID: {1075c5c10803030003i632984c0if846f916aa6a2995-at-mail.gmail.com} 4, 27 -- Date: Mon, 3 Mar 2008 11:03:21 +0300 4, 27 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- Subject: TEM camera problem. 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=UTF-8 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I would like to correct a statement that I made erroneously in a previous email. We buy our re- tipped filaments currently from AGAR not Joel (The new ones come from them sorry for the typo). I apologise to Joel for indicating that there re-tips were some what of a lottery, they do not in fact sell retips.
Many thanks for all of the other responses. Apparently it may be easy(ish) to do with the correct set up so I am going to have to go and have a rummage for the equipment.
Regards John (Bristol University, UK)
==============================Original Headers============================== 4, 27 -- From john.mitchels-at-gmail.com Mon Mar 3 02:20:00 2008 4, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m238JwI4018960 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 02:19:58 -0600 4, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so5377186rvb.30 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 03 Mar 2008 00:19:55 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=+DEISss3dTZK1UBjo10Zqk+MFXLSa9G/RaJZ4H6Ol0I=; 4, 27 -- b=en19xYt4QNVlADmsmbF7O/9Evk1DRmqADLMVRsw7mgLs8r+RncYPzOA/BAUTfcEESElatmOZpuEnFlofzDjmj7ShikVsDRBSW0YSUJitxs5piPCf+Xz/8twHRuuQyuQQ0wJzlONE6Fapzwd13y470fYqhoXlXQY7XiOChKWH8Og= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=eb6lVCvSvYkbKbobBgzZ+i8g+pwxHL1VfoMm0b3F36LtrdLCiHN5NCD57ToSCc/aFJQ/EhUhWLM0n5nNl32GJeQRZsZSpMbfjqr6rVETdowQ+sMzv2iQDoVgB58yAr1vfkXC51hmBU2TqbTkR5Yx7fct0dP9ZYPFuqUTvzhhwxg= 4, 27 -- Received: by 10.141.63.20 with SMTP id q20mr7576957rvk.258.1204532395370; 4, 27 -- Mon, 03 Mar 2008 00:19:55 -0800 (PST) 4, 27 -- Received: by 10.141.170.6 with HTTP; Mon, 3 Mar 2008 00:19:55 -0800 (PST) 4, 27 -- Message-ID: {1b3cf7c30803030019l49b32459k799ec4b905f7c0c1-at-mail.gmail.com} 4, 27 -- Date: Mon, 3 Mar 2008 08:19:55 +0000 4, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- Subject: Re-tipped Filaments Pt3 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I always assumed heat fixing worked on two levels: it fixes the proteins etc.., it was found to work well, and is (or rather was*) very easy to do in the lab with a Bunsen burner. About three seconds is enough for this, over-heat the sample and you probably get just the carbon residue of what was bacteria (a Bunsen flame is 'hot') and possibly black smutty soot from the flame as well. Secondly heat fixing also kills the bacteria, which given the medical importance of most bacteria and their histo-stains, this is generally considered a good thing.
Stains have to get through the cell wall and this is by diffusion (indeed differential diffusion through cell walls of different structure is the basis of the Gram stain). Heat the stain & slide, and the liquid solvent carrier of the stain might boil off the slide leaving a gunky goo on the glass and little stain inside the bacteria (this is a bad thing).
Plus as http://www.fiu.edu/~makemson/MCB2000Lab/Exp2GramStain.pdf points out, just slight overheating during fixation can be a real problem and lead to false Gram stain results:
"Note that the success of the Gram stain relies upon the integrity of the cell wall. Gram positive bacteria that have been overly heat fixed resulting in destruction of all or parts of their cell wall can appear to be pink (Gram negative) or have pink areas. This is an artifact! Further, old moribund cultures of Gram positive cells can appear pink. This is because the cell wall has allowed the challenge rinse to enter the cell. Successful Gram stains should be done on young, growing cells." The above links adds in more details on heat fixation and the Gram staining process as well.
Some stains appreciate a bit of warming, and generally a staining protocol is followed because it works rather than the entire process is completely understood on the atomic level. Histology is as much an art as a science, and the more you do the better you get at it, even though the written method you are following often remains unchanged [and with classical coloured stains it was probably originally developed around 100 years ago].
Regards
Keith
*Our labs have banned naked flames, so the Bunsen is out [hot plates/ovens are in]. Scientists can be trusted to build hydrogen bombs and anti-gravity devices for stealth bombers, but not with a schoolkid's Bunsen burner these days.
Safety note :- after working with any kind of bacteria, once finished, immediately place the slides into a disinfectant solution and wash, wipe work surfaces down with an appropriate disinfectant / antibacterial agent such as 70 % ethanol, sodium hypochlorite (aqueous, 10% sol), or a household disinfectant made up to the manufacturer's directions, and wash hands with an antiseptic soap. Avoid hand contact with the eyes or mouth whilst working with bacteria, and always handle cultures in the correct manner, assume everything is a pathogen.
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: jlbrennan-at-comcast.net [mailto:jlbrennan-at-comcast.net] Sent: 02 March 2008 22:38 To: kjmorris-at-well.ox.ac.uk
This Question was submitted to Ask-A-Microscopist by (jlbrennan-at-comcast.net)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, March 2, 2008 at 16:12:57 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jlbrennan-at-comcast.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: My lab book poses a few questions on heat fixing slide to which I can not find the answers anywhere. I have reviewed the lab book cover to cover, check the related text and have spent the last hour+ doing various Google searchs and the closes I have come to an answer is your site.
We have been making slides to Gram stain in class. The book asks why they have to air dry and why the process can not be sped up by gently heating them in a flame. Can you explain why this can not be done?
Second, can the slide be over heated during the fixing process? If so what happens?
What you have to do after welding the filament on it supoort (and what is done on precentered filament from the manufacturers), is to warm the new filament under vacuum to outgas it and to relax the strains from the W-wire, and those induced by the bending/welding. One put the holder with it new filament freshly welded (or a filament batch) in a vacuum vessel (a all purpouse evaporator, for exemple) and one but some current, typicall 2A, a bit less then in working condition, on the filament for a few minutes or some tenth of minutes.
After that, one mount at each filament on a jig, which simulate the filament holder and wehnelt assembly, and one bend again the filament from what is needed to center correctly the tip on the wehenelt hole. On Jeol K-type, one adust the screws from the stainless steel ring to center the ceramic part, on bend what is needed the filament, and one correct again with the screw what is still necessary. A binocular with transmitted light is a good help, to see the shadow of the tip in the wehnelts's hole.
Not difficult... if one has spot welding apparatus, the jigs for the bending, welding, and centering, the evaporator and all what is needed to hold and connect the filaments in it !
We do this for electron guns used in Auger analysis, which have no internal centering possibility of the filament, and guns for Auger spectrometry do never work at saturated conditions, their filament has to be counted in years. But for SEM or TEM, it needs too much time to be gainfull.
On the other hand, it's interesting to have done this one time, to see and to touch what the worth of pre-centered filament !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } Thank you all for your responses, I'm afraid I have worded my email } rather ambiguously I meant to say that I was interested to know how } easy it is to re tip the filaments myself rather than getting an } external company to do it. I just wondered how it was done and whether } anyone had experience of the process themselves in their own lab. } } Thanks again } John } } ==============================Original Headers============================== } 3, 27 -- From john.mitchels-at-gmail.com Fri Feb 29 09:55:12 2008 } 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) } 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TFtBvg008165 } 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:55:12 -0600 } 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so3650736rvb.30 } 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 07:55:10 -0800 (PST) } 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 3, 27 -- d=gmail.com; s=gamma; } 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 3, 27 -- bh=Uz9LQuOI1uYAKLHS6tv0pyqk7Z6N41djFJ+bGwrsmbI=; } 3, 27 -- b=lrsJdyesmgy+hTyLqECtUyOdaNN2KYEoVWz972rYXZ9pXa05bMgQ40i6d1DE57w+FTSKKueWc5LwBUSKVCHex1WrYGHkx83UYOPHOYwaArzfFVoYaXZGlNuY6p7FNWTa2xoYpxs/V8H+Oi0t3pRw4NIq/VjlRzMIAC+NcIR+Hos= } 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 3, 27 -- d=gmail.com; s=gamma; } 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 3, 27 -- b=vS05uBnfimwIpmf2GY4mmEppAt27IhnrP6yHFWaseM3Onq6KXyLWGDfiks9pfC+TRBT23lyMZMGz9E1SOHBJWUEd3X1m96KT/MH64KVKuu9F0xbDoYDfhVKtYmg9a+8Lpuyb/ghDUTNThhieQ28YYOrbPuSpGYPt7YMJSjFfjns= } 3, 27 -- Received: by 10.141.1.2 with SMTP id d2mr6468194rvi.42.1204300510353; } 3, 27 -- Fri, 29 Feb 2008 07:55:10 -0800 (PST) } 3, 27 -- Received: by 10.141.33.4 with HTTP; Fri, 29 Feb 2008 07:55:10 -0800 (PST) } 3, 27 -- Message-ID: {1b3cf7c30802290755t2637d96bgaef8295c74077323-at-mail.gmail.com} } 3, 27 -- Date: Fri, 29 Feb 2008 15:55:10 +0000 } 3, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} } 3, 27 -- To: Microscopy-at-microscopy.com } 3, 27 -- Subject: Retipped Filaments part 2 } 3, 27 -- MIME-Version: 1.0 } 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 3, 27 -- Content-Transfer-Encoding: 7bit } 3, 27 -- Content-Disposition: inline } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Mar 3 06:58:45 2008 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.158]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23CwhD7021941 12, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 06:58:44 -0600 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m23CwcvP035831 12, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 13:58:38 +0100 (CET) 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id A7781424006 12, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 3 Mar 2008 13:57:45 +0100 (CET) 12, 29 -- Message-ID: {47CBF5CA.90303-at-ipcms.u-strasbg.fr} 12, 29 -- Date: Mon, 03 Mar 2008 13:57:46 +0100 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 12, 29 -- MIME-Version: 1.0 12, 29 -- To: Microscopy-at-microscopy.com 12, 29 -- Subject: Re: [Microscopy] Retipped Filaments part 2 12, 29 -- References: {200802291601.m1TG1kfL016714-at-ns.microscopy.com} 12, 29 -- In-Reply-To: {200802291601.m1TG1kfL016714-at-ns.microscopy.com} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-IPCMS-MailScanner: Found to be clean 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.158]); Mon, 03 Mar 2008 13:58:38 +0100 (CET) 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/6092/Mon Mar 3 06:04:26 2008 on mr8.u-strasbg.fr 12, 29 -- X-Virus-Status: Clean 12, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 12, 29 -- version=3.1.8 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr8.u-strasbg.fr ==============================End of - Headers==============================
Hello, It might be a problem of image clipping. Try to go through the camera and image acquisition setting in your camera software and look for "clipping border" item (or something similar) and set it to "No clipping". Best regards from Prague Oldrich
On 3 Mar 2008 at 2:07, celikaktas-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } Recently, we have bought a new 200kV TEM with a 2k CCD. When we } acquire an image in full frame mode, we observe that five pixels at } the very ends of the CCD are merged into one. This repeats through-out } the circumference of the CCD. So, my image has 2038x2038 pixels of } useful data. In half and quarter mode acquisitions we do not see this } problem. } } Previously, I was using 1k CCD's and never experienced anything like } this. Is there some kind of technical issue with 2k cameras that they } have to give-up five pixels around the CCD? } } Thank you, } Ayten Celik-Aktas. } } ==============================Original Headers============================== } 4, 27 -- From celikaktas-at-gmail.com Mon Mar 3 02:03:23 2008 } 4, 27 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.187]) } 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2383MYb005883 } 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 02:03:22 -0600 } 4, 27 -- Received: by fk-out-0910.google.com with SMTP id 18so9865770fkq.2 } 4, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 03 Mar 2008 00:03:22 -0800 (PST) } 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 27 -- d=gmail.com; s=gamma; } 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 4, 27 -- bh=0OK6Lg6XvMPppGZ5udtOJnHtfiP/v1IhqEdz2uSjjuo=; } 4, 27 -- b=wz+a8DcxRcFgDOkVs7HEUNMMj/VAHuvoIHzusip+ktyVvrAPKY+GZFp8UTaP8DF6LahOtcAwArXYb2rVZB9vLMpM0yGQlxzrZ9QuQB0iOfEoHL/LOGHhp69KyUS+y+QkB3c40rsmpSFVxE8+SRo/miWnORJcqLJzxlZq3Hv4bS8= } 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 27 -- d=gmail.com; s=gamma; } 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 4, 27 -- b=BXFTzFmuUQMFvb3Bm589n41umq9tOFthu9Z/eHmLtN5Zy0smy7AsWBaLuOZn/YvnyJ+aKdxaq2H/Aorn0hReoE+Ti+jH37SUsZaN0PR8hYj6ROduZMLggI7My0yVMOM9Xb5db+UhzQPLvtSF+FRHSpW+YfekTCsKdIqLMyl/MVQ= } 4, 27 -- Received: by 10.82.161.19 with SMTP id j19mr32121808bue.0.1204531401893; } 4, 27 -- Mon, 03 Mar 2008 00:03:21 -0800 (PST) } 4, 27 -- Received: by 10.82.125.5 with HTTP; Mon, 3 Mar 2008 00:03:21 -0800 (PST) } 4, 27 -- Message-ID: {1075c5c10803030003i632984c0if846f916aa6a2995-at-mail.gmail.com} } 4, 27 -- Date: Mon, 3 Mar 2008 11:03:21 +0300 } 4, 27 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} } 4, 27 -- To: Microscopy-at-microscopy.com } 4, 27 -- Subject: TEM camera problem. } 4, 27 -- MIME-Version: 1.0 } 4, 27 -- Content-Type: text/plain; charset=UTF-8 } 4, 27 -- Content-Transfer-Encoding: 7bit } 4, 27 -- Content-Disposition: inline } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 24 -- From benada-at-biomed.cas.cz Mon Mar 3 08:47:10 2008 6, 24 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23El9L7006303 6, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 08:47:10 -0600 6, 24 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 6, 24 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id D3794928018; 6, 24 -- Mon, 3 Mar 2008 15:47:04 +0100 (CET) 6, 24 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 6, 24 -- Organization: Institute of Microbiology 6, 24 -- To: celikaktas-at-gmail.com 6, 24 -- Date: Mon, 03 Mar 2008 15:47:05 +0100 6, 24 -- MIME-Version: 1.0 6, 24 -- Subject: Re: [Microscopy] TEM camera problem. 6, 24 -- CC: Microscopy-at-microscopy.com 6, 24 -- Message-ID: {47CC1D79.14546.2EDD60-at-benada.biomed.cas.cz} 6, 24 -- Priority: normal 6, 24 -- In-reply-to: {200803030807.m2387mYn010063-at-ns.microscopy.com} 6, 24 -- References: {200803030807.m2387mYn010063-at-ns.microscopy.com} 6, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 24 -- Content-type: text/plain; charset=US-ASCII 6, 24 -- Content-transfer-encoding: 7BIT 6, 24 -- Content-description: Mail message body 6, 24 -- X-Antivirus: avast! (VPS 080302-0, 02.03.2008), Outbound message 6, 24 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
Several people have contacted me about expanding the focus of the Open_Cut_Microtome_Project to include other lines of microtomes and that seems like a great idea, especially since many people have said they have and Ultracut and some other microtome(s). So, the group can be considered a forum for discussions and sharing on support of older microtomes in general.
And again, I apologize for the faulty link to the informational public web page. The correct link is: http://people.umass.edu/dac/projects/Open_Cut/ And I will get it updated to include this expanded focus. I have a developing list of the service providers on that web page, and vendors who support the older microtomes are encouraged to join, or send info regarding what they service, and what region they cover, and links to their pages, etc.
And in addition to telling me to add you to the list, I think one can email the group email to request joining. =================================== open_cut_project-at-googlegroups.com http://groups.google.com/group/open_cut_project ===================================
Cheers!
Dale Callaham
==============================Original Headers============================== 7, 20 -- From dac-at-research.umass.edu Mon Mar 3 09:11:11 2008 7, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23FBBxD019645 7, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 09:11:11 -0600 7, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 20 -- (authenticated bits=0) 7, 20 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m23FB6OV007628 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 10:11:07 -0500 7, 20 -- Message-ID: {47CC15F3.5070808-at-research.umass.edu} 7, 20 -- Date: Mon, 03 Mar 2008 10:14:59 -0500 7, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 20 -- Reply-To: dac-at-research.umass.edu 7, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 7, 20 -- MIME-Version: 1.0 7, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 20 -- Subject: Open_Cut Microtome Project is expanding to all older microtomes 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Everyone is invited to attend the Southeastern Microscopy Society (SEMS) annual meeting in sunny, warm, white sandy beach, Pensacola Beach, Florida April 16-18, 2008.
Please see the SEMS website www.southeasternmicroscopy.org for details.
Invited Speakers: Dr. Elaine Schumacher, McCrone Institute Dr. Alan W Nicholls, PhD, The University of Illinois at Chicago Dr. Ingeborg Schmidt-Krey, Georgia Tech, Atlanta GA Dr. Byung-Ho Kang, University of Florida
Banquet Speaker: Dr. Charles Mims, University of Georgia
Tutorials and Demonstrations: Marine Reef International- Hitachi Tabletop SEM JEOL-Remote access SEM and TEM
Workshops: *Cryoultramicrotomy Techniques & Applications in Biological & Materials Science*-Sponsored by Boeckler/RMC
Tour: Tour of McSwain Engineering, Pensacola, Florida, and Demonstration of Hitachi S-3400N and S-3700N microscopes. Sponsored by Hitachi HTA
Platform Presentations: Silicon Drift Detectors - The Future of X-Ray Microanalysis Raman Spectrometry in Biological Analysis The Four A*s of imaging, Analysis, Automation, Acquisition, and Art: Getting the most out of your digital images 3-D reconstruction of FIB manipulated materials What you can do with a Variable Pressure SEM Quantum Dots -TEM Applications
Vendors: Nikon JEOL Olympus EMS Bruker Optics . Bruker AXS Marine Reef International Zeiss SMT Hitachi HTA ICMAS Edax SEMTech Solutions Boeckeler Instruments Gatan S. Bryant Inc Nano and More Tousimis FEI ThermoScientific
Ruska Competition: Established as a tribute to Ernst and Helmut Ruska, students compete for best presentations in the biological and/or physical sciences. Winners are presented with a plaque and monetary award.
Art Competition: Prizes for the best light, SEM, and TEM photos.
Contributed papers are welcome. The deadline for abstract submission is March 10, 2008, and abstracts may be sent to jshields-at-cb.uga.edu or chumphrey-at-cdc.gov. Registration deadline is March 16, 2008.
Hope to see you there!
Robert Simmons, President rbsimmons-at-mindspring.com Giselle Thibaudeau, President-Elect Giselle-at-emcenter.msstate.edu Program Chairs: Charles Humphrey chumphrey-at-cdc.gov John Shields jshields-at-cb.uga.ed Local arrangements: Cynthia Goldsmith csg1-at-cdc.gov Amanda Lawrence alawrence-at-emcenter.msstate.edu Bill Monroe monroe-at-emcenter.msstate.edu; ;
==============================Original Headers============================== 16, 19 -- From ALawrence-at-entomology.msstate.edu Mon Mar 3 09:35:16 2008 16, 19 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23FZFsJ000788 16, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 3 Mar 2008 09:35:16 -0600 16, 19 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 16, 19 -- with Novell_GroupWise; Mon, 03 Mar 2008 09:35:15 -0600 16, 19 -- Message-Id: {47CBC64E.B26A.00E6.0-at-entomology.msstate.edu} 16, 19 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 16, 19 -- Date: Mon, 03 Mar 2008 09:35:10 -0600 16, 19 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 16, 19 -- To: {Microscopy-at-Microscopy.Com} 16, 19 -- Subject: Southeastern Microscopy Society annual meeting 16, 19 -- References: {47CBC52C020000E600035FA4-at-mailhost2.groupwise.msstate.edu} 16, 19 -- {47CBC542020000E600035FA6-at-mailhost2.groupwise.msstate.edu} 16, 19 -- {47CBC64E020000E600035FAB-at-mailhost2.groupwise.msstate.edu} 16, 19 -- Mime-Version: 1.0 16, 19 -- Content-Type: text/plain; charset=ISO-8859-15 16, 19 -- Content-Transfer-Encoding: 8bit 16, 19 -- Content-Disposition: inline ==============================End of - Headers==============================
I can confirm that we also have set the outermost pixel to an average value. For our CCD camera model, this greatly improves the 'quality' of the power spectrum, by the elimination of sharp edges. This feature needs to be set for each camera resolution, so for other settings, it may not exist. best regards, Reinhard --
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 4, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Mon Mar 3 12:02:53 2008 4, 23 -- Received: from rrzmta1.rz.uni-regensburg.de (rrzmta1.rz.uni-regensburg.de [194.94.155.51]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23I2qDA019334 4, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 3 Mar 2008 12:02:53 -0600 4, 23 -- Received: from rrzmta1.rz.uni-regensburg.de (localhost [127.0.0.1]) 4, 23 -- by localhost (Postfix) with SMTP id 7041E4F0F9 4, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 3 Mar 2008 19:02:59 +0100 (CET) 4, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 4, 23 -- by rrzmta1.rz.uni-regensburg.de (Postfix) with ESMTP id 687C34F0D4 4, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 3 Mar 2008 19:02:59 +0100 (CET) 4, 23 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 4, 23 -- with Novell_GroupWise; Mon, 03 Mar 2008 19:02:51 +0100 4, 23 -- Message-Id: {47CC4B53.044C.0054.0-at-biologie.uni-regensburg.de} 4, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 4, 23 -- Date: Mon, 03 Mar 2008 19:02:43 +0100 4, 23 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 4, 23 -- To: {Microscopy-at-Microscopy.Com} 4, 23 -- Subject: TEM camera problem 4, 23 -- Mime-Version: 1.0 4, 23 -- Content-Type: text/plain; charset=US-ASCII 4, 23 -- Content-Disposition: inline 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m23I2qDA019334 ==============================End of - Headers==============================
On Mar 3, 2008, at 12:03 AM, celikaktas-at-gmail.com wrote:
} } Recently, we have bought a new 200kV TEM with a 2k CCD. When we } acquire an image in full frame mode, we observe that five pixels at } the very ends of the CCD are merged into one. This repeats through-out } the circumference of the CCD. So, my image has 2038x2038 pixels of } useful data. In half and quarter mode acquisitions we do not see this } problem. } } Previously, I was using 1k CCD's and never experienced anything like } this. Is there some kind of technical issue with 2k cameras that they } have to give-up five pixels around the CCD? }
Dear Ayten, There are often bad pixels at the edge of CCD chips. With our 2k camera the 8 pixels nearest the border contained enough bad pixels that the camera was configured to set them equal to the values of the pixels just inboard of them. If you have a Gatan camera and DigitalMicrograph, there is a panel you can access that lists the pixels so designated. I'm pretty sure that the same is true for Tietz cameras and software, but I have no experience with them. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 5, 22 -- From tivol-at-caltech.edu Mon Mar 3 12:08:20 2008 5, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23I8J3D024675 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 3 Mar 2008 12:08:20 -0600 5, 22 -- Received: from earth-dog.its.caltech.edu (earth-dog [192.168.1.3]) 5, 22 -- by wood-ox-postvirus (Postfix) with ESMTP id 5C92013E65 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 3 Mar 2008 10:08:18 -0800 (PST) 5, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 5, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 4BD6913E5A 5, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 3 Mar 2008 10:08:15 -0800 (PST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- In-Reply-To: {200803030803.m2383aXl006033-at-ns.microscopy.com} 5, 22 -- References: {200803030803.m2383aXl006033-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {A7A68760-0ACD-4A07-A970-876E93353E14-at-caltech.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 5, 22 -- Subject: Re: [Microscopy] TEM camera problem. 5, 22 -- Date: Mon, 3 Mar 2008 10:08:20 -0800 5, 22 -- To: microscopy-at-msa.microscopy.com 5, 22 -- X-Mailer: Apple Mail (2.753) 5, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Buy a high-quality ink jet printer (Canon or Epson) that will take true black and photo-gray inks. Scan your negatives, adjust with PhotoShop and print with the ink jet. I had an Agfa DD 3700 that made great prints in about 75 seconds but getting chemistry is difficult as there is no market for these machines anymore. The new era is "desktop darkroom". Don't fight it.
Geoff
thatch-at-mit.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both thatch-at-mit.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: thatch-at-mit.edu } Name: Tori Hatch } } Organization: MIT/HHMI } } Title-Subject: [Filtered] black and white print processor } } Question: Hello, } } I am looking for a black and white print processor to print 8.5 x 10 } images. If anyone is still manufacturing these I'd like to know } about them, but it appears that they are rapidly becoming dinasaurs. } If you have a used machine with reasonably good (or replaceable) } rollers to sell please contact me. } } Thanks, } Tori Hatch } Horvitz Lab } MIT / HHMI } 617-253-6138 } } Login Host: 18.79.2.128 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Fri Feb 22 16:57:52 2008 } 8, 11 -- Received: from [10.59.1.8] (msdvpn8.msd.anl.gov [130.202.238.72]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1MMvnvL013595 } 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 22 Feb 2008 16:57:51 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240808c3e503dc22da-at-[10.59.1.8]} } 8, 11 -- Date: Sat, 23 Feb 2008 09:57:51 +1100 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: thatch-at-mit.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: black and white print processor } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 28 -- From mcauliff-at-umdnj.edu Mon Mar 3 13:17:15 2008 9, 28 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23JHFKx014548 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 13:17:15 -0600 9, 28 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 9, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E1B054BEE0 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 14:17:11 -0500 (EST) 9, 28 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 9, 28 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 198564BEC9 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 14:17:11 -0500 (EST) 9, 28 -- Received: from ([10.32.15.171]) 9, 28 -- by imail.umdnj.edu with ESMTP id 5502050.8182949; 9, 28 -- Mon, 03 Mar 2008 14:16:49 -0500 9, 28 -- MIME-version: 1.0 9, 28 -- Content-transfer-encoding: 7BIT 9, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 9, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 9, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0JX6007MQ5K0EW30-at-umduwc02.umdnj.edu} for 9, 28 -- microscopy-at-microscopy.com; Mon, 03 Mar 2008 14:16:49 -0500 (EST) 9, 28 -- Message-id: {47CC4EBF.9050303-at-umdnj.edu} 9, 28 -- Date: Mon, 03 Mar 2008 14:17:19 -0500 9, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 28 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 9, 28 -- To: thatch-at-mit.edu, microscopy-at-microscopy.com 9, 28 -- Subject: Re: [Microscopy] viaWWW: black and white print processor 9, 28 -- References: {200802222259.m1MMxmMK018873-at-ns.microscopy.com} 9, 28 -- In-reply-to: {200802222259.m1MMxmMK018873-at-ns.microscopy.com} ==============================End of - Headers==============================
It comes me in mind, I've forgotten somthing in my former mail.
One tool more is necessary to completely rebuild the filament set. The alumina (or melted glass) holder needs to be cleaned from the W deposit coming from the filament evaporation. Otherwise, one will have with time a leakage current that will give a bad control of the filament current maybe until a short. This cleaning is done with a sandblaster, used with fine alumina or SiC powder. It gives a nice white surface, a bit matter than when new.
Bon courage !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
jacques.faerber-at-ipcms.u-strasbg.fr a écrit : } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } On cent more to want Mary has told : } } What you have to do after welding the filament on it supoort (and what } is done on precentered filament from the manufacturers), is to warm the } new filament under vacuum to outgas it and to relax the strains from the } W-wire, and those induced by the bending/welding. One put the holder } with it new filament freshly welded (or a filament batch) in a vacuum } vessel (a all purpouse evaporator, for exemple) and one but some } current, typicall 2A, a bit less then in working condition, on the } filament for a few minutes or some tenth of minutes. } } After that, one mount at each filament on a jig, which simulate the } filament holder and wehnelt assembly, and one bend again the filament } from what is needed to center correctly the tip on the wehenelt hole. On } Jeol K-type, one adust the screws from the stainless steel ring to } center the ceramic part, on bend what is needed the filament, and one } correct again with the screw what is still necessary. A binocular with } transmitted light is a good help, to see the shadow of the tip in the } wehnelts's hole. } } Not difficult... if one has spot welding apparatus, the jigs for the } bending, welding, and centering, the evaporator and all what is needed } to hold and connect the filaments in it ! } } We do this for electron guns used in Auger analysis, which have no } internal centering possibility of the filament, and guns for Auger } spectrometry do never work at saturated conditions, their filament has } to be counted in years. But for SEM or TEM, it needs too much time to be } gainfull. } } On the other hand, it's interesting to have done this one time, to see } and to touch what the worth of pre-centered filament ! } } Hope it helps } } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } john.mitchels-at-gmail.com a écrit : } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear Listers } } } } Thank you all for your responses, I'm afraid I have worded my email } } rather ambiguously I meant to say that I was interested to know how } } easy it is to re tip the filaments myself rather than getting an } } external company to do it. I just wondered how it was done and whether } } anyone had experience of the process themselves in their own lab. } } } } Thanks again } } John } } } } ==============================Original Headers============================== } } 3, 27 -- From john.mitchels-at-gmail.com Fri Feb 29 09:55:12 2008 } } 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) } } 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1TFtBvg008165 } } 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 09:55:12 -0600 } } 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so3650736rvb.30 } } 3, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Feb 2008 07:55:10 -0800 (PST) } } 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 3, 27 -- d=gmail.com; s=gamma; } } 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } } 3, 27 -- bh=Uz9LQuOI1uYAKLHS6tv0pyqk7Z6N41djFJ+bGwrsmbI=; } } 3, 27 -- b=lrsJdyesmgy+hTyLqECtUyOdaNN2KYEoVWz972rYXZ9pXa05bMgQ40i6d1DE57w+FTSKKueWc5LwBUSKVCHex1WrYGHkx83UYOPHOYwaArzfFVoYaXZGlNuY6p7FNWTa2xoYpxs/V8H+Oi0t3pRw4NIq/VjlRzMIAC+NcIR+Hos= } } 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 3, 27 -- d=gmail.com; s=gamma; } } 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } } 3, 27 -- b=vS05uBnfimwIpmf2GY4mmEppAt27IhnrP6yHFWaseM3Onq6KXyLWGDfiks9pfC+TRBT23lyMZMGz9E1SOHBJWUEd3X1m96KT/MH64KVKuu9F0xbDoYDfhVKtYmg9a+8Lpuyb/ghDUTNThhieQ28YYOrbPuSpGYPt7YMJSjFfjns= } } 3, 27 -- Received: by 10.141.1.2 with SMTP id d2mr6468194rvi.42.1204300510353; } } 3, 27 -- Fri, 29 Feb 2008 07:55:10 -0800 (PST) } } 3, 27 -- Received: by 10.141.33.4 with HTTP; Fri, 29 Feb 2008 07:55:10 -0800 (PST) } } 3, 27 -- Message-ID: {1b3cf7c30802290755t2637d96bgaef8295c74077323-at-mail.gmail.com} } } 3, 27 -- Date: Fri, 29 Feb 2008 15:55:10 +0000 } } 3, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} } } 3, 27 -- To: Microscopy-at-microscopy.com } } 3, 27 -- Subject: Retipped Filaments part 2 } } 3, 27 -- MIME-Version: 1.0 } } 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } } 3, 27 -- Content-Transfer-Encoding: 7bit } } 3, 27 -- Content-Disposition: inline } } ==============================End of - Headers============================== } } } } } } ==============================Original Headers============================== } 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Mar 3 06:58:45 2008 } 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.158]) } 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m23CwhD7021941 } 12, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 06:58:44 -0600 } 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) } 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m23CwcvP035831 } 12, 29 -- for {microscopy-at-microscopy.com} ; Mon, 3 Mar 2008 13:58:38 +0100 (CET) } 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id A7781424006 } 12, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 3 Mar 2008 13:57:45 +0100 (CET) } 12, 29 -- Message-ID: {47CBF5CA.90303-at-ipcms.u-strasbg.fr} } 12, 29 -- Date: Mon, 03 Mar 2008 13:57:46 +0100 } 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 12, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) } 12, 29 -- MIME-Version: 1.0 } 12, 29 -- To: Microscopy-at-microscopy.com } 12, 29 -- Subject: Re: [Microscopy] Retipped Filaments part 2 } 12, 29 -- References: {200802291601.m1TG1kfL016714-at-ns.microscopy.com} } 12, 29 -- In-Reply-To: {200802291601.m1TG1kfL016714-at-ns.microscopy.com} } 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 29 -- Content-Transfer-Encoding: 8bit } 12, 29 -- X-IPCMS-MailScanner: Found to be clean } 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr } 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.158]); Mon, 03 Mar 2008 13:58:38 +0100 (CET) } 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/6092/Mon Mar 3 06:04:26 2008 on mr8.u-strasbg.fr } 12, 29 -- X-Virus-Status: Clean } 12, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled } 12, 29 -- version=3.1.8 } 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr8.u-strasbg.fr } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Mar 4 01:39:24 2008 8, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.151]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m247dNSt018428 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 01:39:23 -0600 8, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m247dIRd023767 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 08:39:18 +0100 (CET) 8, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id C1A5342400D 8, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Mar 2008 08:38:28 +0100 (CET) 8, 29 -- Message-ID: {47CCFC7A.9030509-at-ipcms.u-strasbg.fr} 8, 29 -- Date: Tue, 04 Mar 2008 08:38:34 +0100 8, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 29 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080227) 8, 29 -- MIME-Version: 1.0 8, 29 -- To: Microscopy-at-microscopy.com 8, 29 -- Subject: Retipped Filaments part 2 + something more 8, 29 -- References: {200803031307.m23D7bVI030646-at-ns.microscopy.com} 8, 29 -- In-Reply-To: {200803031307.m23D7bVI030646-at-ns.microscopy.com} 8, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 29 -- Content-Transfer-Encoding: 8bit 8, 29 -- X-IPCMS-MailScanner: Found to be clean 8, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.151]); Tue, 04 Mar 2008 08:39:18 +0100 (CET) 8, 29 -- X-Virus-Scanned: ClamAV 0.88.7/6120/Tue Mar 4 08:08:30 2008 on mr1.u-strasbg.fr 8, 29 -- X-Virus-Status: Clean 8, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 8, 29 -- version=3.1.8 8, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr1.u-strasbg.fr ==============================End of - Headers==============================
Hi folks, I'm trying to align a series of FIB/SEM images, which have been acquired using a FIB slice/SEM image/FIB slice... sequence. Due to drift (during an overnight run) there are some quite large shifts to account for.
Now I know biologists have been doing this kind of thing for yonks, so there must be plenty of cross-correlation packages out there. What do you recommend? ImageJ and Digital Micrograph plugins/macros would be the preferred option.
Many thanks
Richard Beanland
==============================Original Headers============================== 5, 22 -- From rb258-at-hermes.cam.ac.uk Tue Mar 4 07:29:38 2008 5, 22 -- Received: from ppsw-7.csi.cam.ac.uk (ppsw-7.csi.cam.ac.uk [131.111.8.137]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24DTckg017078 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 07:29:38 -0600 5, 22 -- X-Cam-SpamDetails: Not scanned 5, 22 -- X-Cam-AntiVirus: No virus found 5, 22 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 5, 22 -- Received: from canigou.msm.cam.ac.uk ([131.111.102.43]:1259) 5, 22 -- by ppsw-7.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.157]:587) 5, 22 -- with esmtpsa (PLAIN:rb258) (TLSv1:DHE-RSA-AES256-SHA:256) 5, 22 -- id 1JWXD5-0004m3-Pn (Exim 4.67) for microscopy-at-microscopy.com 5, 22 -- (return-path {rb258-at-hermes.cam.ac.uk} ); Tue, 04 Mar 2008 13:29:36 +0000 5, 22 -- Message-ID: {47CD4F5D.1090409-at-integrityscientific.com} 5, 22 -- Date: Tue, 04 Mar 2008 13:32:13 +0000 5, 22 -- From: Richard Beanland {contact-at-integrityscientific.com} 5, 22 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- Subject: Serial section alignment 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Sender: "Dr R. Beanland" {rb258-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eric.leroy-at-icmpe.cnrs.fr as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy
Organization: CNRS
Title-Subject: [Filtered] SIS TIFF images
Question: We have a SIS Megaview III camera attached to our Tecnai F20. The images produced by this camera are stored with the Analysis software that stores the spatial calibration with proprietary tags. From this, we are able to open the images with any imaging software (like ImageJ for example) but without the spatial calibration. Does anybody wrote a software or better an ImageJ macro or plugin to read the spatial calibration of TIFF SIS images?
Good Day: I have managed to freeze the samples, get them into the chuck of the microtome and trim them, I even get sections but I can't get the sections from the edge of the knife to the grid. They end up as little snow balls. I've been trying with an eyelash to lift or flick them over. Does anyone have a tip as to how this can be done reliably? Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Tue Mar 4 08:49:22 2008 5, 26 -- Received: from gigi.cs.uoguelph.ca (gigi.cs.uoguelph.ca [131.104.94.210]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24EnMYH012842 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 08:49:22 -0600 5, 26 -- Received: from legolas.cs.uoguelph.ca (legolas.cs.uoguelph.ca [131.104.94.56]) 5, 26 -- by gigi.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKid014501 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKqv010668 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Tue, 04 Mar 2008 09:49:20 -0500 5, 26 -- Message-ID: {20080304094920.hnv7ujrp1cscc48k-at-webmail.uoguelph.ca} 5, 26 -- Date: Tue, 04 Mar 2008 09:49:20 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Picking up cryo-sections 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.210 ==============================End of - Headers==============================
Hi I use AutoAligner by Bitplane. It does not fit your below stated requirements, but it is very good at doing my TEM images. http://www.bitplane.com/go/products/autoaligner
David
On Mar 4, 2008, at 6:34 AM, contact-at-integrityscientific.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi folks, } I'm trying to align a series of FIB/SEM images, which have been } acquired using a FIB slice/SEM image/FIB slice... sequence. Due to } drift (during an overnight run) there are some quite large shifts to } account for. } } Now I know biologists have been doing this kind of thing for yonks, } so } there must be plenty of cross-correlation packages out there. What do } you recommend? ImageJ and Digital Micrograph plugins/macros would be } the preferred option. } } Many thanks } } Richard Beanland } } } ==============================Original } Headers============================== } 5, 22 -- From rb258-at-hermes.cam.ac.uk Tue Mar 4 07:29:38 2008 } 5, 22 -- Received: from ppsw-7.csi.cam.ac.uk (ppsw-7.csi.cam.ac.uk } [131.111.8.137]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m24DTckg017078 } 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 07:29:38 } -0600 } 5, 22 -- X-Cam-SpamDetails: Not scanned } 5, 22 -- X-Cam-AntiVirus: No virus found } 5, 22 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ } 5, 22 -- Received: from canigou.msm.cam.ac.uk ([131.111.102.43]:1259) } 5, 22 -- by ppsw-7.csi.cam.ac.uk (smtp.hermes.cam.ac.uk } [131.111.8.157]:587) } 5, 22 -- with esmtpsa (PLAIN:rb258) (TLSv1:DHE-RSA-AES256-SHA:256) } 5, 22 -- id 1JWXD5-0004m3-Pn (Exim 4.67) for } microscopy-at-microscopy.com } 5, 22 -- (return-path {rb258-at-hermes.cam.ac.uk} ); Tue, 04 Mar 2008 } 13:29:36 +0000 } 5, 22 -- Message-ID: {47CD4F5D.1090409-at-integrityscientific.com} } 5, 22 -- Date: Tue, 04 Mar 2008 13:32:13 +0000 } 5, 22 -- From: Richard Beanland {contact-at-integrityscientific.com} } 5, 22 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) } 5, 22 -- MIME-Version: 1.0 } 5, 22 -- To: microscopy-at-microscopy.com } 5, 22 -- Subject: Serial section alignment } 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 22 -- Content-Transfer-Encoding: 7bit } 5, 22 -- Sender: "Dr R. Beanland" {rb258-at-hermes.cam.ac.uk} } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 22 -- From Elliott-at-arizona.edu Tue Mar 4 09:10:44 2008 6, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.arizona.edu [128.196.133.168]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24FAhw8025986 6, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 09:10:43 -0600 6, 22 -- Received: from frodos_amavis (amavis6.email.arizona.edu [10.0.0.209]) 6, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 5908D2E3E74 6, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 08:10:43 -0700 (MST) 6, 22 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 6, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 528242CCCDE 6, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 08:10:39 -0700 (MST) 6, 22 -- Message-Id: {A9ABCFC6-6FB3-4B88-8EAF-41E1E0DE6625-at-arizona.edu} 6, 22 -- From: David Elliott {Elliott-at-arizona.edu} 6, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 22 -- In-Reply-To: {200803041334.m24DY1Br021846-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v919.2) 6, 22 -- Subject: Re: [Microscopy] Serial section alignment 6, 22 -- Date: Tue, 4 Mar 2008 08:10:34 -0700 6, 22 -- References: {200803041334.m24DY1Br021846-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.919.2) 6, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
There are a couple of factors that may be causing your problems. 1. Static electricity - are you using an ionizer? 2. Speed of cutting - Either max out the cutting speed and pull off ribbons, catching them on an eyelash as soon as they begin forming. OR use a micromanipulator (Ladinsky 2006) and lower the cutting speed to 0.1-0.2mm/sec.
Cheers, KD Derr New York Structural Biology Center
-----Original Message----- X-from: bharris-at-uoguelph.ca [mailto:bharris-at-uoguelph.ca] Sent: Tuesday, March 04, 2008 9:52 AM To: kderr-at-nysbc.org
Good Day: I have managed to freeze the samples, get them into the chuck of the microtome and trim them, I even get sections but I can't get the sections from the edge of the knife to the grid. They end up as little snow balls. I've been trying with an eyelash to lift or flick them over. Does anyone have a tip as to how this can be done reliably? Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Tue Mar 4 08:49:22 2008 5, 26 -- Received: from gigi.cs.uoguelph.ca (gigi.cs.uoguelph.ca [131.104.94.210]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24EnMYH012842 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 08:49:22 -0600 5, 26 -- Received: from legolas.cs.uoguelph.ca (legolas.cs.uoguelph.ca [131.104.94.56]) 5, 26 -- by gigi.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKid014501 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKqv010668 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Tue, 04 Mar 2008 09:49:20 -0500 5, 26 -- Message-ID: {20080304094920.hnv7ujrp1cscc48k-at-webmail.uoguelph.ca} 5, 26 -- Date: Tue, 04 Mar 2008 09:49:20 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Picking up cryo-sections 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.210 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 23 -- From kderr-at-nysbc.org Tue Mar 4 09:15:15 2008 15, 23 -- Received: from nysbc.org (nysbc.org [74.211.206.2]) 15, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24FFAdD002399 15, 23 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:15:11 -0600 15, 23 -- Received: from CRYOEM03 ([10.10.10.1]) 15, 23 -- by nysbc.org (Kerio MailServer 6.5.0); 15, 23 -- Tue, 4 Mar 2008 10:19:33 -0500 15, 23 -- From: "kd Derr" {kderr-at-nysbc.org} 15, 23 -- To: {bharris-at-uoguelph.ca} 15, 23 -- Cc: {microscopy-at-microscopy.com} 15, 23 -- Subject: RE: [Microscopy] TEM: Picking up cryo-sections 15, 23 -- Date: Tue, 4 Mar 2008 10:13:26 -0500 15, 23 -- Message-ID: {008a01c87e0a$4b9fb670$7f05a8c0-at-CRYOEM03} 15, 23 -- MIME-Version: 1.0 15, 23 -- Content-Type: text/plain; 15, 23 -- charset="US-ASCII" 15, 23 -- Content-Transfer-Encoding: 7bit 15, 23 -- X-Priority: 3 (Normal) 15, 23 -- X-MSMail-Priority: Normal 15, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 15, 23 -- In-Reply-To: {200803041452.m24EqAwx017959-at-ns.microscopy.com} 15, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1914 15, 23 -- Importance: Normal ==============================End of - Headers==============================
I use a loop, dip it in my cryoprotectant (2.3M Sucrose with 10% PVP in my case) and lift the sections on the liquid. Then put the sections (and the drop of liquid) on the grid (make sure the grid is not too hydrophobic). I then invert the grid and float it on a dish of my cryoprotectant. The sections don't desiccate that way and when the section is picked up on the liquid-filled loop the spread out.
David
On Mar 4, 2008, at 7:52 AM, bharris-at-uoguelph.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good Day: I have managed to freeze the samples, get them into the } chuck of the microtome and trim them, I even get sections but I can't } get the sections from the edge of the knife to the grid. They end up } as little snow balls. I've been trying with an eyelash to lift or } flick them over. Does anyone have a tip as to how this can be done } reliably? Thanks bob harris } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } ==============================Original } Headers============================== } 5, 26 -- From bharris-at-uoguelph.ca Tue Mar 4 08:49:22 2008 } 5, 26 -- Received: from gigi.cs.uoguelph.ca (gigi.cs.uoguelph.ca } [131.104.94.210]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m24EnMYH012842 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 08:49:22 } -0600 } 5, 26 -- Received: from legolas.cs.uoguelph.ca } (legolas.cs.uoguelph.ca [131.104.94.56]) } 5, 26 -- by gigi.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } m24EnKid014501 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 } -0500 } 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain } [127.0.0.1]) } 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } m24EnKqv010668 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 } -0500 } 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by } webmail.uoguelph.ca } 5, 26 -- (Horde MIME library) with HTTP; Tue, 04 Mar 2008 09:49:20 } -0500 } 5, 26 -- Message-ID: {20080304094920.hnv7ujrp1cscc48k-at-webmail.uoguelph.ca } } } 5, 26 -- Date: Tue, 04 Mar 2008 09:49:20 -0500 } 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} } 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 5, 26 -- Subject: TEM: Picking up cryo-sections } 5, 26 -- MIME-Version: 1.0 } 5, 26 -- Content-Type: text/plain; } 5, 26 -- charset=ISO-8859-1; } 5, 26 -- DelSp="Yes"; } 5, 26 -- format="flowed" } 5, 26 -- Content-Disposition: inline } 5, 26 -- Content-Transfer-Encoding: 7bit } 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.210 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 7, 22 -- From Elliott-at-arizona.edu Tue Mar 4 09:15:58 2008 7, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.arizona.edu [128.196.133.168]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24FFu5X004003 7, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 09:15:57 -0600 7, 22 -- Received: from frodos_amavis (amavis2.email.arizona.edu [10.0.0.205]) 7, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 9D6192E3E74 7, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 08:15:55 -0700 (MST) 7, 22 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 7, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id D28E92E4452 7, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 08:15:52 -0700 (MST) 7, 22 -- Message-Id: {8845995E-EE19-4711-8017-5E1C658483FC-at-arizona.edu} 7, 22 -- From: David Elliott {Elliott-at-arizona.edu} 7, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 7, 22 -- In-Reply-To: {200803041452.m24Eq05G017563-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Mime-Version: 1.0 (Apple Message framework v919.2) 7, 22 -- Subject: Re: [Microscopy] TEM: Picking up cryo-sections 7, 22 -- Date: Tue, 4 Mar 2008 08:15:45 -0700 7, 22 -- References: {200803041452.m24Eq05G017563-at-ns.microscopy.com} 7, 22 -- X-Mailer: Apple Mail (2.919.2) 7, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Bob- Try the Tokyasu method: use a small platinum loop that has been dipped in 2.3M sucrose, hover it near the knife until it just starts to Microscopy Listserver {microscopy-at-microscopy.com} ,gel, then press the sucrose against the sections. Remove the loop from the chamber and allow the drop to thaw. Now, press the bottom of the drop against your grid to transfer the sections there. If you need to keep the sections frozen, this method is no good for you, and I must defer to others who deal in the world of continuously frozen stuff. Lee
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A colleague is looking to purchase a microscope to use in demos at local high schools. One experiment she wants to do is look at daphnia before and after exposure to caffeine. Apparently the heart rate really kicks up when they get a dose of espresso...
Is phase contrast necessary to image daphnia? Koeller illumination?
Thanks, Doug
..................................................................... Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy Assist. Scientific Investigator University of Arizona (office: AHSC 4212A) P.O. Box 245044 (voice: 520-626-2824) Tucson, AZ 85724-5044 USA (FAX: 520-626-2097) (email: Cromey-at-Arizona.edu) ..................................................................... http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
==============================Original Headers============================== 8, 26 -- From dcromey-at-email.arizona.edu Tue Mar 4 11:17:15 2008 8, 26 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24HHFZ5015582 8, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Mar 2008 11:17:15 -0600 8, 26 -- Received: from gandalfs_amavis (amavis3.email.arizona.edu [10.0.0.206]) 8, 26 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 08FDB47D7E8 8, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Mar 2008 10:17:15 -0700 (MST) 8, 26 -- Received: from localhost (hornet.email.arizona.edu [10.0.0.234]) 8, 26 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 099FA47D7CF 8, 26 -- for {microscopy-at-msa.microscopy.com} ; Tue, 4 Mar 2008 10:17:14 -0700 (MST) 8, 26 -- Received: from hepatic1.cba.arizona.edu (hepatic1.cba.arizona.edu 8, 26 -- [128.196.157.6]) by www.email.arizona.edu (Horde) with HTTP for 8, 26 -- {dcromey-at-email.arizona.edu} ; Tue, 4 Mar 2008 10:17:14 -0700 8, 26 -- Message-ID: {20080304101714.3yurcw84w0kgos00-at-www.email.arizona.edu} 8, 26 -- X-Priority: 3 (Normal) 8, 26 -- Date: Tue, 4 Mar 2008 10:17:14 -0700 8, 26 -- From: Doug Cromey {dcromey-at-email.arizona.edu} 8, 26 -- Reply-to: cromey-at-arizona.edu 8, 26 -- To: Microscopy_Listserv {microscopy-at-msa.microscopy.com} 8, 26 -- Subject: Daphnia - phase contrast? 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; charset="ISO-8859-1" 8, 26 -- Content-Disposition: inline 8, 26 -- Content-Transfer-Encoding: 7bit 8, 26 -- User-Agent: Internet Messaging Program (IMP) 4.0-cvs 8, 26 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Strangely enough for all the precision engineered and expensive equipment required for cryothin sectioning, it all comes down to the pickup. For my two bits, when I get a ribbon or a single section laying down on the knife face I use a platinum wire loop dipped in 2.3M sucrose in 0.1M phosphate buffer for high compression tissues such as skin (this allows them to stretch back out) or a mix of 1 part 2% methylcellulose and 2 parts 2.3M sucrose for low compression tissues such as thymus (this keeps them from stretching). It seems to work best for me if the droplet is fairly flat, not bulging with pickup solution. The pickup for sucrose must be fairly quick, about 2 seconds. If the droplet is frozen when it contacts the section, the section will have poor morphology. The drop should still be jellylike where it is cold enough that it doesn’t stick to the knife face like syrup. The methylcellulose mix gives you more time (perhaps three seconds) and gives you the added feature of smoking (vapor) when you put the loop into the cold chamber. The section should be picked up right when it vapor stops. In both cases the pickup should be a quick, smooth and a gentle press of the droplet against the sections. Don’t hesitate and allow the sections to fly up toward the loop (this might be the snowball). By the time the loop is retrieved, the droplet will be frozen. Have your grid (face up) on a clean surface and after waiting about 10 seconds for thawing, slowly contact the grid with the loop. The sections will adhere to the grid. I usually continue straight to immunolabeling so I gently place the grids face down on my solution of PBS with 0.5% BSA for the subsequent immunogold. I hope this helps.
Robert Underwood University of Washington Dermatology
On Tue, 4 Mar 2008 bharris-at-uoguelph.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good Day: I have managed to freeze the samples, get them into the } chuck of the microtome and trim them, I even get sections but I can't } get the sections from the edge of the knife to the grid. They end up } as little snow balls. I've been trying with an eyelash to lift or } flick them over. Does anyone have a tip as to how this can be done } reliably? Thanks bob harris } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } ==============================Original Headers============================== } 5, 26 -- From bharris-at-uoguelph.ca Tue Mar 4 08:49:22 2008 } 5, 26 -- Received: from gigi.cs.uoguelph.ca (gigi.cs.uoguelph.ca [131.104.94.210]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24EnMYH012842 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 08:49:22 -0600 } 5, 26 -- Received: from legolas.cs.uoguelph.ca (legolas.cs.uoguelph.ca [131.104.94.56]) } 5, 26 -- by gigi.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKid014501 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 } 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) } 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKqv010668 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 } 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca } 5, 26 -- (Horde MIME library) with HTTP; Tue, 04 Mar 2008 09:49:20 -0500 } 5, 26 -- Message-ID: {20080304094920.hnv7ujrp1cscc48k-at-webmail.uoguelph.ca} } 5, 26 -- Date: Tue, 04 Mar 2008 09:49:20 -0500 } 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} } 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 5, 26 -- Subject: TEM: Picking up cryo-sections } 5, 26 -- MIME-Version: 1.0 } 5, 26 -- Content-Type: text/plain; } 5, 26 -- charset=ISO-8859-1; } 5, 26 -- DelSp="Yes"; } 5, 26 -- format="flowed" } 5, 26 -- Content-Disposition: inline } 5, 26 -- Content-Transfer-Encoding: 7bit } 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.210 } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 24 -- From underwoo-at-u.washington.edu Tue Mar 4 11:43:04 2008 10, 24 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24Hh32M028962 10, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 11:43:04 -0600 10, 24 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103]) 10, 24 -- by mxout5.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m24Hh2pf010986 10, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 10, 24 -- Tue, 4 Mar 2008 09:43:02 -0800 10, 24 -- Received: from localhost (localhost [127.0.0.1]) 10, 24 -- by hymn13.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m24Hh2rk025699; 10, 24 -- Tue, 4 Mar 2008 09:43:02 -0800 10, 24 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Tue, 04 Mar 2008 09:43:02 PST 10, 24 -- Date: Tue, 4 Mar 2008 09:43:02 -0800 (PST) 10, 24 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 10, 24 -- To: bharris-at-uoguelph.ca, Microscopy List {Microscopy-at-MSA.Microscopy.Com} 10, 24 -- Subject: [Microscopy] Re: TEM: Picking up cryo-sections 10, 24 -- In-Reply-To: {200803041450.m24EoQvc014761-at-ns.microscopy.com} 10, 24 -- Message-ID: {Pine.LNX.4.43.0803040943020.12983-at-hymn13.u.washington.edu} 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: TEXT/PLAIN; charset=ISO-8859-1; format=flowed 10, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.4.93048 10, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='SUPERLONG_LINE 0.05, __C230066_P5 0, __CP_POSSIBLE_EXPLOIT_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NUM 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id m24Hh32M028962 ==============================End of - Headers==============================
Phase isn't needed for Daphnia, and in fact would likely be uselss. Too much "halo" on a thick specimen. DIC would be much better, or Hoffman Modulation. Daphnia show up fine in plain brightfield, but one of the interference methods (DIC or Hoffman) would help to clearly image the heart through all the guts, appendages, and other impedimentia.
Phil
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==============================Original Headers============================== 4, 23 -- From oshel1pe-at-cmich.edu Tue Mar 4 12:20:08 2008 4, 23 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24IK7qP010841 4, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 12:20:07 -0600 4, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 23 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m24IZ69N016217; 4, 23 -- Tue, 4 Mar 2008 13:35:07 -0500 4, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 23 -- Tue, 4 Mar 2008 13:19:54 -0500 4, 23 -- Mime-Version: 1.0 4, 23 -- Message-Id: {f06240809c3f342173f63-at-[141.209.160.249]} 4, 23 -- In-Reply-To: {200803041722.m24HMxDC022071-at-ns.microscopy.com} 4, 23 -- References: {200803041722.m24HMxDC022071-at-ns.microscopy.com} 4, 23 -- Date: Tue, 4 Mar 2008 13:20:02 -0500 4, 23 -- To: dcromey-at-email.arizona.edu 4, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 23 -- Subject: Re: [Microscopy] Daphnia - phase contrast? 4, 23 -- Cc: Microscopy-at-microscopy.com 4, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 23 -- X-OriginalArrivalTime: 04 Mar 2008 18:19:54.0748 (UTC) FILETIME=[586C63C0:01C87E24] 4, 23 -- X-CanItPRO-Stream: default 4, 23 -- X-Spam-Score: -4 () L_EXCH_MF 4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
There is an assumption in the replies that the poster is working with fixed and cryoprotected material. However, there is a clue in the original message that suggests the specimens may be rapidly-frozen and fully hydrated ("I have managed to freeze the samples, get them into the chuck of the microtome and trim them").
If the specimens are unfixed and not cryoprotected, non of the retrieval methods will work. However, using an anti-static line (deionizer) should help spread the sections out, but only if the knife is very sharp (and perhaps a diamond). The correct temperature is also important for sectioning un-fixed, un-cryoprotected material. I think the cryochamber should be held around -125 degrees for sectioning vitrified specimens, but it has been a long time since I did any of this.
Maybe the poster of the original message could clarify what specimens are being sectioned before we attempt an answer.
Regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {bharris-at-uoguelph.ca} } Reply-To: {bharris-at-uoguelph.ca} } Date: Tue, 4 Mar 2008 08:52:41 -0600 } To: {pwebster-at-hei.org} } Subject: [Microscopy] TEM: Picking up cryo-sections } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good Day: I have managed to freeze the samples, get them into the } chuck of the microtome and trim them, I even get sections but I can't } get the sections from the edge of the knife to the grid. They end up } as little snow balls. I've been trying with an eyelash to lift or } flick them over. Does anyone have a tip as to how this can be done } reliably? Thanks bob harris } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } ==============================Original Headers============================== } 5, 26 -- From bharris-at-uoguelph.ca Tue Mar 4 08:49:22 2008 } 5, 26 -- Received: from gigi.cs.uoguelph.ca (gigi.cs.uoguelph.ca } [131.104.94.210]) } 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m24EnMYH012842 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 08:49:22 -0600 } 5, 26 -- Received: from legolas.cs.uoguelph.ca (legolas.cs.uoguelph.ca } [131.104.94.56]) } 5, 26 -- by gigi.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m24EnKid014501 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 } 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain } [127.0.0.1]) } 5, 26 -- by legolas.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } m24EnKqv010668 } 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 09:49:20 -0500 } 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by } webmail.uoguelph.ca } 5, 26 -- (Horde MIME library) with HTTP; Tue, 04 Mar 2008 09:49:20 -0500 } 5, 26 -- Message-ID: {20080304094920.hnv7ujrp1cscc48k-at-webmail.uoguelph.ca} } 5, 26 -- Date: Tue, 04 Mar 2008 09:49:20 -0500 } 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} } 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 5, 26 -- Subject: TEM: Picking up cryo-sections } 5, 26 -- MIME-Version: 1.0 } 5, 26 -- Content-Type: text/plain; } 5, 26 -- charset=ISO-8859-1; } 5, 26 -- DelSp="Yes"; } 5, 26 -- format="flowed" } 5, 26 -- Content-Disposition: inline } 5, 26 -- Content-Transfer-Encoding: 7bit } 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.210 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 20 -- From PWebster-at-hei.org Tue Mar 4 12:51:37 2008 11, 20 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24Iparm025266 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 12:51:37 -0600 11, 20 -- Received: from 10.10.42.80 ([10.10.42.80]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 11, 20 -- Tue, 4 Mar 2008 18:51:33 +0000 11, 20 -- User-Agent: Microsoft-Entourage/11.4.0.080122 11, 20 -- Date: Tue, 04 Mar 2008 10:51:30 -0800 11, 20 -- Subject: Re: [Microscopy] TEM: Picking up cryo-sections 11, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 11, 20 -- To: {bharris-at-uoguelph.ca} 11, 20 -- CC: {microscopy-at-microscopy.com} 11, 20 -- Message-ID: {C3F2DA32.177C5%PWebster-at-hei.org} 11, 20 -- Thread-Topic: [Microscopy] TEM: Picking up cryo-sections 11, 20 -- Thread-Index: Ach+KMIUANAHAuocEdyaBQANk7Zh7g== 11, 20 -- In-Reply-To: {200803041452.m24EqfFd019013-at-ns.microscopy.com} 11, 20 -- Mime-version: 1.0 11, 20 -- Content-type: text/plain; 11, 20 -- charset="US-ASCII" 11, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
I am exploring methods to build high resolution 3D images of small fossil teeth where accurate measurements of tooth morphology can be made. If anyone has done this type of work or could recommend a method, I would love to hear from you.
Thanks
Roy Beavers
Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 23 -- From rbeavers-at-mail.smu.edu Tue Mar 4 13:14:16 2008 7, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24JEFWI006229 7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 13:14:16 -0600 7, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Tue, 4 Mar 2008 13:14:17 -0600 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: 3D SEM Images 7, 23 -- Date: Tue, 4 Mar 2008 13:14:17 -0600 7, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B303E57270-at-s31xe7.systems.smu.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: 3D SEM Images 7, 23 -- Thread-Index: Ach+K/An0BKaPU57RM63A1yOFt7htg== 7, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 04 Mar 2008 19:14:17.0508 (UTC) FILETIME=[F12E0E40:01C87E2B] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m24JEFWI006229 ==============================End of - Headers==============================
I'm trying to help someone who has some formalin-fixed tissue sections that have been cryoprotected in a 30% ethylene glycol/20% glycerol solution; we'd like to process the tissue for laser microdissection for purposes of RNA extraction. The difficulty is that cryoprotectant tends to absorb the laser wavelengths and heat the tissue during the cutting process, causing damage and decreasing the liklihood of successful RNA extraction (the likelihood of which has already been reduced by the fixation process).
I would imagine that there must be a way to de-cryoprotect through successive washes in a miscible solvent, I'm not sure if this would cause even greater complications with RNA work, or if fairly gentle standard protocols exist so I'm appealing to the collective knowledge of this list:) Has anyone experienced a need to remove cryoprotectant from tissue (brain) sections and are there perhaps methods to accomplish this that might be somewhat amenable to later extraction of RNA from select features in the tissue sections?
Thanks in advance for any advice or helpful hints.
Sincere Regards, Karl -- Karl Garsha Research Microscopy Specialist US-Southwest Region Leica Microsystems-Life Sciences Research www.leica-microsystems.com
==============================Original Headers============================== 5, 26 -- From garsha-at-itg.uiuc.edu Tue Mar 4 15:45:49 2008 5, 26 -- Received: from outmail137065.authsmtp.com (outmail137065.authsmtp.com [62.13.137.65]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24LjmkL026058 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 15:45:49 -0600 5, 26 -- Received: from outmail128177.authsmtp.com (outmail128177.authsmtp.com [62.13.128.177]) 5, 26 -- by punt4.authsmtp.com (8.14.1/8.14.1/Kp) with ESMTP id m24Lis4G096692 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 21:44:54 GMT 5, 26 -- Received: from [192.168.1.45] (ip68-110-1-221.tc.ph.cox.net [68.110.1.221]) 5, 26 -- (authenticated bits=0) 5, 26 -- by mail.authsmtp.com (8.13.8/8.13.8/Kp) with ESMTP id m24LjeOM066134 5, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 21:45:41 GMT 5, 26 -- Message-ID: {47CDC306.60300-at-itg.uiuc.edu} 5, 26 -- Date: Tue, 04 Mar 2008 14:45:42 -0700 5, 26 -- From: Karl Garsha {garsha-at-itg.uiuc.edu} 5, 26 -- Reply-To: karl.garsha-at-leica-microsystems.com 5, 26 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 5, 26 -- MIME-Version: 1.0 5, 26 -- To: microscopy-at-microscopy.com 5, 26 -- Subject: removing cryoprotectant from fixed tissue 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- X-Server-Quench: 5601aa41-ea34-11dc-9670-001185d377ca 5, 26 -- X-AuthRoute: OCdyaAgTClZaRR4B CiosDTNPDxgkOxYK DBMeOw5bK0AOTg9W KldyK1tYKloHTlZB SnhYBAkaUFluJDI3 dAhRbQ1NYEtEWwBg VEkHRFpMFQRoHx0f BBwfTRt3dQBZeDAt Z0IeBCUkIUR8c0d+ RgBVE2UFK2JmaGFO AURZalYFcwdXfx4R OU12UnQNfGUHZ3to QgNoYG86NCNFJSFS XAALIhoZW1cMBiQ7 Wx0JATwpAQULXSI2 ZxInOlMQVFoQKV4v PDNh 5, 26 -- X-Authentic-SMTP: 61633138303639.squirrel.dmpriest.net.uk:787/Kp 5, 26 -- X-Report-SPAM: If SPAM / abuse - report it at: http://www.authsmtp.com/abuse 5, 26 -- X-Virus-Status: No virus detected - but ensure you scan with your own anti-virus system! ==============================End of - Headers==============================
Energy Beam Sciences is the U.S. master distributor for MeX, a software product produced by Alicona Imaging. MeX is a 3-D imaging and metrology software solution for SEM applications. The product is designed to provide the specific type of capabilities that you are looking for.
Contact Jef Glen at jglen-at-ebsciences.com, 800-992-9037 (x342) for complete details.
rbeavers-at-mail.smu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Group, } } I am exploring methods to build high resolution 3D images of small } fossil teeth where accurate measurements of tooth morphology can be } made. If anyone has done this type of work or could recommend a method, } I would love to hear from you. } } Thanks } } Roy Beavers } } Southern Methodist University } Department of Earth Sciences } P.O. Box 750395 } Dallas, TX 75275 } Voice: 214-768-2756 } Fax: 214-768-2701 } Email: rbeavers-at-smu.edu } } } } ==============================Original Headers============================== } 7, 23 -- From rbeavers-at-mail.smu.edu Tue Mar 4 13:14:16 2008 } 7, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24JEFWI006229 } 7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 13:14:16 -0600 } 7, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); } 7, 23 -- Tue, 4 Mar 2008 13:14:17 -0600 } 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 7, 23 -- Content-class: urn:content-classes:message } 7, 23 -- MIME-Version: 1.0 } 7, 23 -- Content-Type: text/plain; } 7, 23 -- charset="us-ascii" } 7, 23 -- Subject: 3D SEM Images } 7, 23 -- Date: Tue, 4 Mar 2008 13:14:17 -0600 } 7, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B303E57270-at-s31xe7.systems.smu.edu} } 7, 23 -- X-MS-Has-Attach: } 7, 23 -- X-MS-TNEF-Correlator: } 7, 23 -- Thread-Topic: 3D SEM Images } 7, 23 -- Thread-Index: Ach+K/An0BKaPU57RM63A1yOFt7htg== } 7, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} } 7, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} } 7, 23 -- X-OriginalArrivalTime: 04 Mar 2008 19:14:17.0508 (UTC) FILETIME=[F12E0E40:01C87E2B] } 7, 23 -- Content-Transfer-Encoding: 8bit } 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m24JEFWI006229 } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 7, 27 -- From mdufraine-at-ebsciences.com Tue Mar 4 16:04:58 2008 7, 27 -- Received: from mx-outbound01.easydns.com (mailout1.easydns.com [205.210.42.66]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m24M4weL006973 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 16:04:58 -0600 7, 27 -- Received: from mail.ebsciences.com (65-115-7-18.dia.static.qwest.net [65.115.7.18]) 7, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 27 -- (No client certificate requested) 7, 27 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 0240E482AF; 7, 27 -- Tue, 4 Mar 2008 17:04:41 -0500 (EST) 7, 27 -- Received: from mdufraine.ebsciences.private ([10.10.0.190]) 7, 27 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 7, 27 -- (Exim 4.68) 7, 27 -- (envelope-from {mdufraine-at-ebsciences.com} ) 7, 27 -- id 1JWfFp-0007gK-Lx; Tue, 04 Mar 2008 17:04:57 -0500 7, 27 -- Message-ID: {47CDC789.9080903-at-ebsciences.com} 7, 27 -- Date: Tue, 04 Mar 2008 17:04:57 -0500 7, 27 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 7, 27 -- Organization: Energy Beam Sciences 7, 27 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 7, 27 -- MIME-Version: 1.0 7, 27 -- To: rbeavers-at-mail.smu.edu 7, 27 -- CC: microscopy-at-microscopy.com 7, 27 -- Subject: Re: [Microscopy] 3D SEM Images 7, 27 -- References: {200803041915.m24JF3PO007281-at-ns.microscopy.com} 7, 27 -- In-Reply-To: {200803041915.m24JF3PO007281-at-ns.microscopy.com} 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I may be misunderstanding the question - do you mean that you want the scale bar saved with your image in a form that can be read by other programmes? If so, it can be burnt into the image by the SIS software and saved that way. Let me know, if that is what you want, if you need further help to set this.
Diana
Diana van Driel
Discipline of Ophthalmology Sydney University GPO Box 4337 Sydney, NSW AUSTRALIA 2001
On 5 Mar 2008, at 1:34 AM, eric.leroy-at-icmpe.cnrs.fr wrote:
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==============================Original Headers============================== 11, 20 -- From dianavd-at-eye.usyd.edu.au Tue Mar 4 19:49:57 2008 11, 20 -- Received: from galen.med.usyd.edu.au (nugalen.med.usyd.edu.au [129.78.36.39]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m251nu5J026123 11, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 19:49:56 -0600 11, 20 -- Received: from [129.78.203.144] (helo=[129.78.203.144]) 11, 20 -- by galen.med.usyd.edu.au with esmtp (Exim 4.43) 11, 20 -- id 1JWilV-0004CH-Mu 11, 20 -- for Microscopy-at-microscopy.com; Wed, 05 Mar 2008 12:49:55 +1100 11, 20 -- Mime-Version: 1.0 (Apple Message framework v753) 11, 20 -- In-Reply-To: {200803041434.m24EYJUF001746-at-ns.microscopy.com} 11, 20 -- References: {200803041434.m24EYJUF001746-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 20 -- Message-Id: {669ED561-8398-4FEC-B938-BB6952408532-at-eye.usyd.edu.au} 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au} 11, 20 -- Subject: Re: [Microscopy] viaWWW: SIS TIFF images 11, 20 -- Date: Wed, 5 Mar 2008 12:49:43 +1100 11, 20 -- To: Microscopy {Microscopy-at-microscopy.com} 11, 20 -- X-Mailer: Apple Mail (2.753) 11, 20 -- X-Spam-Score: -1.4 (-) ==============================End of - Headers==============================
On 5 Mar 2008, at 12:32 AM, contact-at-integrityscientific.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi folks, } I'm trying to align a series of FIB/SEM images, which have been } acquired using a FIB slice/SEM image/FIB slice... sequence. Due to } drift (during an overnight run) there are some quite large shifts } to account for. } } Now I know biologists have been doing this kind of thing for } yonks, so } there must be plenty of cross-correlation packages out there. What do } you recommend? ImageJ and Digital Micrograph plugins/macros would be } the preferred option. } } Many thanks } } Richard Beanland } } } ==============================Original } Headers============================== } 5, 22 -- From rb258-at-hermes.cam.ac.uk Tue Mar 4 07:29:38 2008 } 5, 22 -- Received: from ppsw-7.csi.cam.ac.uk (ppsw-7.csi.cam.ac.uk } [131.111.8.137]) } 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m24DTckg017078 } 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 07:29:38 } -0600 } 5, 22 -- X-Cam-SpamDetails: Not scanned } 5, 22 -- X-Cam-AntiVirus: No virus found } 5, 22 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ } 5, 22 -- Received: from canigou.msm.cam.ac.uk ([131.111.102.43]:1259) } 5, 22 -- by ppsw-7.csi.cam.ac.uk (smtp.hermes.cam.ac.uk } [131.111.8.157]:587) } 5, 22 -- with esmtpsa (PLAIN:rb258) (TLSv1:DHE-RSA-AES256-SHA:256) } 5, 22 -- id 1JWXD5-0004m3-Pn (Exim 4.67) for } microscopy-at-microscopy.com } 5, 22 -- (return-path {rb258-at-hermes.cam.ac.uk} ); Tue, 04 Mar 2008 } 13:29:36 +0000 } 5, 22 -- Message-ID: {47CD4F5D.1090409-at-integrityscientific.com} } 5, 22 -- Date: Tue, 04 Mar 2008 13:32:13 +0000 } 5, 22 -- From: Richard Beanland {contact-at-integrityscientific.com} } 5, 22 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) } 5, 22 -- MIME-Version: 1.0 } 5, 22 -- To: microscopy-at-microscopy.com } 5, 22 -- Subject: Serial section alignment } 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 22 -- Content-Transfer-Encoding: 7bit } 5, 22 -- Sender: "Dr R. Beanland" {rb258-at-hermes.cam.ac.uk} } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 20 -- From dianavd-at-eye.usyd.edu.au Tue Mar 4 19:55:00 2008 9, 20 -- Received: from galen.med.usyd.edu.au (nugalen.med.usyd.edu.au [129.78.36.39]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m251sxhg032234 9, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 19:55:00 -0600 9, 20 -- Received: from [129.78.203.144] (helo=[129.78.203.144]) 9, 20 -- by galen.med.usyd.edu.au with esmtp (Exim 4.43) 9, 20 -- id 1JWiqP-00066J-2f 9, 20 -- for Microscopy-at-microscopy.com; Wed, 05 Mar 2008 12:54:59 +1100 9, 20 -- Mime-Version: 1.0 (Apple Message framework v753) 9, 20 -- In-Reply-To: {200803041332.m24DW5uJ019208-at-ns.microscopy.com} 9, 20 -- References: {200803041332.m24DW5uJ019208-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 20 -- Message-Id: {4DC34579-E823-473A-80D3-1895888FE8FF-at-eye.usyd.edu.au} 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au} 9, 20 -- Subject: Re: [Microscopy] Serial section alignment 9, 20 -- Date: Wed, 5 Mar 2008 12:54:51 +1100 9, 20 -- To: Microscopy {Microscopy-at-microscopy.com} 9, 20 -- X-Mailer: Apple Mail (2.753) 9, 20 -- X-Spam-Score: -1.2 (-) ==============================End of - Headers==============================
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Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] SEM X-section polishing
Question: I've recently acquired an old Buehler lapping wheel and am experimenting with a few x-section techniques for SEM work. I am using a final polishing cloth and .05um collodial silica for the final step and the suspension is sticking to the metal wiring. Despite my efforts to clean the surface (with acetone, DDwater, ect.), I can't free the surface of the remaining suspension material. Any advice in regards to freeing the surface of the sticky material will be greatly appreciated!!
Also, I read the recent inquiry and a few of the responses in regard to the removal or the Au/Pd coating for a biological specimen. What does one recommend for the coating removal for a material sample???
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Email: mgondo-at-optometry.uh.edu Name: Margaret Gondo
Organization: University of Houston College of Optometry
Title-Subject: [Filtered] getting service for a RMC MT-7000
Question: Does anyone know of a good service person to come in and service a RMC MT-7000 Ultramicrotome? We're having problems advancing/retracting.
It is very important to not let the colloidal silica dry on your sample. Once it dries it becomes very difficult to remove. South Bay Technology (www.southbaytech.com) does sell a sample cleaner that works very well at removing colloidal silica from samples. Try contacting them, you may be able to get a sample for testing. Of course, I think a bottle of the stuff is only about $10 so you may as well just buy some!
I hope this helps.
Best regards-
David
David Henriks Impact Scientific 32158 Camino Capistrano, Suite A #429 San Juan Capistrano, CA 92675
-----Original Message----- X-from: mlibbee-at-gmail.com [mailto:mlibbee-at-gmail.com] Sent: Tuesday, March 04, 2008 7:07 PM To: Henriks-at-cox.net
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Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] SEM X-section polishing
Question: I've recently acquired an old Buehler lapping wheel and am experimenting with a few x-section techniques for SEM work. I am using a final polishing cloth and .05um collodial silica for the final step and the suspension is sticking to the metal wiring. Despite my efforts to clean the surface (with acetone, DDwater, ect.), I can't free the surface of the remaining suspension material. Any advice in regards to freeing the surface of the sticky material will be greatly appreciated!!
Also, I read the recent inquiry and a few of the responses in regard to the removal or the Au/Pd coating for a biological specimen. What does one recommend for the coating removal for a material sample???
As you have discovered, SIS stores all this wonderful data in non-standard TIF tags in the header. One may read these data (and most of the others) using an IMAGING-C macro or module. The simple macro included below will prompt the user for a file name to store the active image as a TIF file and write the data to a second file with the same name and extension '.ini' These files may be read with the standard Win 32API functions from C, C++, and even Visual Basic. You could also write your own file format (with low level IO routines) that you could parse in your image processing program, but I find .ini files convenient. Anyway, this should give you an idea of how to do what you want. This could be put into an Imaging-C module and activated with a tool-button from analySIS.
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==============================Original Headers============================== 9, 22 -- From jrminter-at-rochester.rr.com Tue Mar 4 21:34:39 2008 9, 22 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m253Ydsh018792 9, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Mar 2008 21:34:39 -0600 9, 22 -- Received: from parrotxp ([69.207.155.112]) by hrndva-omta01.mail.rr.com 9, 22 -- with ESMTP 9, 22 -- id {20080305033439.TGTQ28731.hrndva-omta01.mail.rr.com-at-parrotxp} 9, 22 -- for {Microscopy-at-Microscopy.Com} ; Wed, 5 Mar 2008 03:34:39 +0000 9, 22 -- From: "John Minter" {jrminter-at-rochester.rr.com} 9, 22 -- To: {Microscopy-at-Microscopy.Com} 9, 22 -- References: {200803050150.m251oDPU026288-at-ns.microscopy.com} 9, 22 -- In-Reply-To: {200803050150.m251oDPU026288-at-ns.microscopy.com} 9, 22 -- Subject: [Microscopy] SIS TIFF images 9, 22 -- Date: Tue, 4 Mar 2008 22:34:19 -0500 9, 22 -- Message-ID: {000601c87e71$cc52c5c0$64f85140$-at-rr.com} 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; 9, 22 -- charset="windows-1250" 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Mailer: Microsoft Office Outlook 12.0 9, 22 -- Thread-Index: Ach+Y0EnwV1F1CaYRlC8NpnLlRGxLwADS8XQ 9, 22 -- Content-Language: en-us ==============================End of - Headers==============================
Marissa: I know what you mean! David Henriks is correct, do not let the co-Si dry on the sample. What I do is rinse the sample very well with running DI water, then add a drop of soap/detergent solution to the sample surface, then scrub *gently* with a cotton swab saturated with the soap/detergent solution, rinse again in running DI water and then dry with clean (no oil!) compressed air or dry N2. I use dry N2, but my building is plumbed with it. The scrubbing motion is basically rolling the saturated swab over the surface once or twice. For the soap/detergent, I use Micro-Organic soap from Allied High Tech, but Joy (or any other you might have) dishwashing liquid works well, also. One or two drops in about 25mL of DI water is a good mix. (Alconox would probably work, too.) You just need something to break the surface tension of the water.
For Au/Pd removal from a materials sample: if it's a cross-section face, just re-polish it. If your sputter coater has an etch capability, that's good also. For other things, you can try using KI+I2 etch. Recipe: 2.3 grams potassium iodide, 0.65 grams of Iodine and 50 mL of DI water. Stir until all solids are dissolved. This keeps almost indefinitely in a glass bottle. Be aware that this etch will attach Al and Cu to some extent. If you are coating something for the SEM that you know you'll need to do something else to, I recommend using carbon (if you have it). That will come off with an oxygen plasma in just a few seconds with no damage to your circuitry.
Drop me a line if you have any questions.
mlibbee-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa Libbee } } Title-Subject: [Filtered] SEM X-section polishing } } Question: I've recently acquired an old Buehler lapping wheel and am experimenting with a few x-section techniques for SEM work. I am using a final polishing cloth and .05um collodial silica for the final step and the suspension is sticking to the metal wiring. Despite my efforts to clean the surface (with acetone, DDwater, ect.), I can't free the surface of the remaining suspension material. Any advice in regards to freeing the surface of the sticky material will be greatly appreciated!! } } Also, I read the recent inquiry and a few of the responses in regard to the removal or the Au/Pd coating for a biological specimen. What does one recommend for the coating removal for a material sample??? } } Thanks! } } } } Login Host: 63.163.107.100 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Mar 4 20:58:24 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m252wNXK020796 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 20:58:23 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c3f3bcc3af08-at-[206.69.208.22]} } 8, 11 -- Date: Tue, 4 Mar 2008 20:58:22 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: SEM X-section polishing } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } }
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==============================Original Headers============================== 6, 24 -- From r-holdford-at-ti.com Tue Mar 4 23:30:31 2008 6, 24 -- Received: from bear.ext.ti.com (bear.ext.ti.com [192.94.94.41]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m255UU87014640 6, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 4 Mar 2008 23:30:31 -0600 6, 24 -- Received: from dlep32.itg.ti.com ([157.170.170.70]) 6, 24 -- by bear.ext.ti.com (8.13.7/8.13.7) with ESMTP id m255U5cU020606 6, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 24 -- Tue, 4 Mar 2008 23:30:15 -0600 6, 24 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 6, 24 -- by dlep32.itg.ti.com (8.13.7/8.13.7) with ESMTP id m255U4Gu028234; 6, 24 -- Tue, 4 Mar 2008 23:30:04 -0600 (CST) 6, 24 -- Message-ID: {47CE2FDB.6050703-at-ti.com} 6, 24 -- Date: Tue, 04 Mar 2008 23:30:03 -0600 6, 24 -- From: Becky Holdford {r-holdford-at-ti.com} 6, 24 -- Organization: SC Packaging Development -- FA Development 6, 24 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 6, 24 -- MIME-Version: 1.0 6, 24 -- To: mlibbee-at-gmail.com 6, 24 -- CC: MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 24 -- Subject: Re: [Microscopy] SEM X-section polishing 6, 24 -- References: {200803050258.m252wg1t021080-at-ns.microscopy.com} 6, 24 -- In-Reply-To: {200803050258.m252wg1t021080-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
on behalf of one of my colleagues, I'm looking for a seminar on 'failure analysis' (with an emphasis on fracture surface evaluation) in the UK (preferably... the budget...) or in the US.
I'd be gratefull for recommendations/suggestions.
Kind regards, Jens Lietzau
Electron Microscopy, X-Ray Diffraction & Spectroscopy Research & Product Development Centre Lohmar GKN Driveline International GmbH --------------------------------------------------------
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==============================Original Headers============================== 9, 25 -- From Jens.Lietzau-at-gkndriveline.com Wed Mar 5 03:30:34 2008 9, 25 -- Received: from Mail.gkndriveline.com (mail.gkndriveline.com [63.85.32.37]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m259UX9W003710 9, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 03:30:33 -0600 9, 25 -- Received: from MAILSRV13.DL.ADD.INT ([10.150.16.148] RDNS failed) by Mail.gkndriveline.com with Microsoft SMTPSVC(6.0.3790.3959); Wed, 5 Mar 2008 04:30:32 -0500 9, 25 -- Importance: normal 9, 25 -- Priority: normal 9, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4133 9, 25 -- Content-class: urn:content-classes:message 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; 9, 25 -- charset="us-ascii" 9, 25 -- Disposition-Notification-To: "Jens Lietzau" {Jens.Lietzau-at-gkndriveline.com} 9, 25 -- Subject: looking for failure analysis seminar in the UK (or US) 9, 25 -- Date: Wed, 5 Mar 2008 10:30:29 +0100 9, 25 -- Message-ID: {FF86EB43A37DBD4CBB64E85F075AD27401390A33-at-MAILSRV13.DL.ADD.INT} 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- Thread-Topic: looking for failure analysis seminar in the UK (or US) 9, 25 -- Thread-Index: Ach+o42RiUhAbNV2T8GnBDbQM1i44Q== 9, 25 -- From: "Jens Lietzau" {Jens.Lietzau-at-gkndriveline.com} 9, 25 -- To: {Microscopy-at-microscopy.com} 9, 25 -- X-OriginalArrivalTime: 05 Mar 2008 09:30:32.0524 (UTC) FILETIME=[8F1360C0:01C87EA3] 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m259UX9W003710 ==============================End of - Headers==============================
Hi Marissa, just one thing to add to the other replies, which I didn't see in them: collodal silica will precipitate (i.e. stop being colloidal) if the pH becomes at all acid. So the thing to do is to remove all the silica before this happens. Also, if the solution dries the silica will agglomerate. A mildly alkaline detergent works well - I usually use Decon 90 - and I wash/clean the sample in a weak solution straight off the polishing wheel, before it sees any DI water or other cleaning agent.
And thanks to all who replied about aligning serial sections. IMOD looks good (but I personally find the unix needed to get it going rather painful); if I manage to find a brand new version of PhotoShop I will give it a go; but for ease of use (and very impressive performance) the ImageJ plugin StackReg from epfl won hands down (from web link to a sub-pixel aligned image stack in about 20 minutes). Thanks!!
Richard Beanland
mlibbee-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa Libbee } } Title-Subject: [Filtered] SEM X-section polishing } } Question: I've recently acquired an old Buehler lapping wheel and am experimenting with a few x-section techniques for SEM work. I am using a final polishing cloth and .05um collodial silica for the final step and the suspension is sticking to the metal wiring. Despite my efforts to clean the surface (with acetone, DDwater, ect.), I can't free the surface of the remaining suspension material. Any advice in regards to freeing the surface of the sticky material will be greatly appreciated!! } } Also, I read the recent inquiry and a few of the responses in regard to the removal or the Au/Pd coating for a biological specimen. What does one recommend for the coating removal for a material sample??? } } Thanks! } } } } Login Host: 63.163.107.100 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Mar 4 20:58:24 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m252wNXK020796 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 4 Mar 2008 20:58:23 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c3f3bcc3af08-at-[206.69.208.22]} } 8, 11 -- Date: Tue, 4 Mar 2008 20:58:22 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: SEM X-section polishing } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== } }
==============================Original Headers============================== 6, 24 -- From rb258-at-hermes.cam.ac.uk Wed Mar 5 04:26:19 2008 6, 24 -- Received: from ppsw-1.csi.cam.ac.uk (ppsw-1.csi.cam.ac.uk [131.111.8.131]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m25AQIeA019046 6, 24 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 04:26:18 -0600 6, 24 -- X-Cam-SpamDetails: Not scanned 6, 24 -- X-Cam-AntiVirus: No virus found 6, 24 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 6, 24 -- Received: from canigou.msm.cam.ac.uk ([131.111.102.43]:1050) 6, 24 -- by ppsw-1.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.151]:587) 6, 24 -- with esmtpsa (PLAIN:rb258) (TLSv1:DHE-RSA-AES256-SHA:256) 6, 24 -- id 1JWqpB-0002zs-4E (Exim 4.67) 6, 24 -- (return-path {rb258-at-hermes.cam.ac.uk} ); Wed, 05 Mar 2008 10:26:13 +0000 6, 24 -- Message-ID: {47CE75E3.8030700-at-integrityscientific.com} 6, 24 -- Date: Wed, 05 Mar 2008 10:28:51 +0000 6, 24 -- From: Richard Beanland {contact-at-integrityscientific.com} 6, 24 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) 6, 24 -- MIME-Version: 1.0 6, 24 -- To: mlibbee-at-gmail.com, microscopy-at-microscopy.com 6, 24 -- Subject: Re: [Microscopy] viaWWW: SEM X-section polishing (+thanks) 6, 24 -- References: {200803050308.m2538DCo009637-at-ns.microscopy.com} 6, 24 -- In-Reply-To: {200803050308.m2538DCo009637-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- Sender: "Dr R. Beanland" {rb258-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
I have a question pertaining to the calculation of a shape factor from sedigraph and laser particle size distribution data. Does anyone know the relationship equation being used for the calculation or can point me to a source for it? Apparently years back these calculations were being made here but no one seems to recall how to go about it.
Thanks in advance for your help
George R Munzing Jr. BASF Catalysts R&D 25 Middlesex-Essex Tpk Iselin NJ 08830 732-205-7030 email: george.munzing-at-basf.com
==============================Original Headers============================== 4, 22 -- From george.munzing-at-basf.com Wed Mar 5 08:09:47 2008 4, 22 -- Received: from basfemgw2.basf-corp.com (basfemgw2.basf-corp.com [144.29.129.8]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m25E9kS9012690 4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 08:09:46 -0600 4, 22 -- Received: from basfimgw2.andipc.basf-corp.com (basfimgw2.basf-corp.com [144.29.129.9]) 4, 22 -- by basfemgw2.basf-corp.com (8.13.6/8.13.6) with ESMTP id m25E9kcI023946 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 09:09:46 -0500 (EST) 4, 22 -- Received: from basf-corp-gw99.na.basf.com (basf-corp-gw99.na.basf.com [10.195.132.173]) 4, 22 -- by basfimgw2.andipc.basf-corp.com (8.14.1/8.14.1) with ESMTP id m25E9kMp028074 4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 09:09:46 -0500 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- Subject: Need some help with shape factor calculations from PSD data 4, 22 -- MIME-Version: 1.0 4, 22 -- X-Mailer: Lotus Notes Release 6.5.5 CCH2 April 14, 2006 4, 22 -- Message-ID: {OF14B51A50.BB08C675-ON85257403.004DF9A6-05257403.004DCA13-at-notes.basf-corp.com} 4, 22 -- From: George Munzing {george.munzing-at-basf.com} 4, 22 -- Date: Wed, 5 Mar 2008 09:08:43 -0500 4, 22 -- X-MIMETrack: Serialize by Router on BASF-CORP-GW99/BASF-CORP/BASF(Release 6.5.5FP2|October 4, 22 -- 23, 2006) at 03/05/2008 09:09:46 AM, 4, 22 -- Serialize complete at 03/05/2008 09:09:46 AM 4, 22 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
46th Annual Conference 21 – 25 July 2008 University of Botswana
First ever to be hosted outside SA borders!
On behalf of the Microscopy Society of Southern Africa and the University of Botswana, we have the pleasure of inviting you to attend the 46th Annual Conference of MSSA.
We are looking forward receiving you!
MSSA Pre-Conf. Workshops: Monday 21st – Tuesday 22nd July 2008 MSSA Conference: Wednesday 23rd – Friday 25th July 2008
This bold decision to host MSSA in Botswana, also resulted in the natural choice to choose the mild winter climate (0°C - 27°C ) for the event – enabling delegates to optimise the opportunity by incorporating some "safariing" through one of the finest tourist destinations on the African continent. Gaborone, the capital of Botswana, is often cited as the world's fastest growing city. Named after Kgosi Gaborone - a leader who arrived with his people in this area at the end of the 18th century. Gabs, as it is affectionately known, lies off Botswana's safari circuit - it's in the more populous and better-watered southwest - and is usually seen as a stopover for self-drives safari's. On the mainland and among the islands in the Okavango Delta lion, hyenas, wild dog, buffalo, hippo and crocodiles congregate with a teeming variety of antelope and other smaller animals - warthog, mongoose, spotted genets, monkeys, bush babies and tree squirrels. The Central Kalahari Game Reserve, second largest protected area in the world, offers untamed expanses. In the area surrounding the Okavango Delta visitors will also find unforgettable beauty of the more than 400 species of birds. The best gateway to enter Botswana is via Johannesburg International Airport with regular connections throughout the whole world. Air Botswana flies regularly between Johannesburg, Harare and Victoria Falls. Provisional Scientific Programme - Microscopy in nanotechnology - Applications of Cs Corrected HRTEM in Materials Science - Microscopy in oxidation, corrosion, wear and failure analysis - Advances and applications in scanned probe microscopy - Characterisation and development of platinum group alloys (precious metals) - Microscopy in geology/mineralogy - Advances and applications of X-ray spectrometry - Advances in microscope instrumentation and techniques - Specimen preparation for Microscopy - Structural and Molecular Biology - Morphology and Functions - Quality control in microscopy and facility management - Applications of EM in Materials Characterization
INTERNATIONAL SPEAKERS: Thirtieth John Matthews Memorial Lecturer: Prof John Humphreys, School of Materials, University of Manchester. Boris Balinsky Lecture: Prof Mark Yeager from the Department of Cell Biology Scripps Research Institute, La Jolla, USA Invited Speaker: Dr. Marek Faryna, Institute of Metallurgy and Materials Engineering, Kraków, Poland;
PRECONFERENCE WORKSHOPS: Phase diagram course Optical and thin sectioning sample polishing course. Principles and practice of light microscopy TEM Digital imaging (OSIS) Conference Secretary: Mr Stephan Coetzee, MSSA 2008 c/o Electron Microscopy Unit University of Botswana Private Bag X22, Gabarone
The problem at hand is to look very closely at pairs of high-resolution SEM images on the computer monitor. I've managed to piece together two sets of six images each with the hugin open-source panoramic image processing software, but I find that I can see the details in those images only at 100% (pixel-for-pixel) resolution on the monitor of my PC. The process is made more complicated by the need to see small differences in contour on a generally spherical concave surface.
The two montages were made with about a 45 degree difference in viewing angle between the two sets, thereby making a stereo pair. The extreme parallax ought to provide the needed exaggeration in apparent surface relief.
The object under study is a less-than-hemispherical metal socket made with a hemispherical-ended drill. I'm looking at the details of the machining marks in the bowl-shaped socket. Think of looking at your dog's water bowl from just above the rim.
I can open both images in browser (or image-editing) windows that are part-screen (i.e., not maximized) and spaced to match my interpupillary distance, but to pan them I have to switch control from one image to the other in order to pan each image in turn ... that's painfully tedious and distracting.
Is there a way to bring two windows at once under the control of one mouse ? If that were the case, I could view my stereo pairs anywhere within the field of view, right on the monitor, and avoid a lot of cutting and pasting of the original pair of images into a large number of individual pairs that could be placed just 75mm or so apart.
I'm using linux/debian/etch on this PC, but I can use a WinXP laptop in a pinch ...
Thanks, George
George Langford, Sc.D. Principal Consultant Amenex Associates, Inc. amenex-at-amenex.com http://www.amenex.com/
==============================Original Headers============================== 9, 25 -- From amenex-at-amenex.com Wed Mar 5 09:04:31 2008 9, 25 -- Received: from c60.cesmail.net (c60.cesmail.net [216.154.195.49]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m25F4Vft007666 9, 25 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 5 Mar 2008 09:04:31 -0600 9, 25 -- Received: from unknown (HELO delta2) ([192.168.1.50]) 9, 25 -- by c60.cesmail.net with ESMTP; 05 Mar 2008 10:04:30 -0500 9, 25 -- Received: from pool-72-94-185-157.phlapa.fios.verizon.net 9, 25 -- (pool-72-94-185-157.phlapa.fios.verizon.net [72.94.185.157]) by 9, 25 -- webmail.spamcop.net (Horde MIME library) with HTTP; Wed, 05 Mar 2008 9, 25 -- 10:04:30 -0500 9, 25 -- Message-ID: {20080305100430.8ip7pu58g0so4c8c-at-webmail.spamcop.net} 9, 25 -- Date: Wed, 05 Mar 2008 10:04:30 -0500 9, 25 -- From: "George Langford, Sc.D." {amenex-at-amenex.com} 9, 25 -- To: Microscopy-at-msa.microscopy.com 9, 25 -- Cc: "George Langford, Sc.D." {amenex-at-amenex.com} 9, 25 -- Subject: Viewing montages with simultaneous panning 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; 9, 25 -- charset=ISO-8859-1; 9, 25 -- DelSp="Yes"; 9, 25 -- format="flowed" 9, 25 -- Content-Disposition: inline 9, 25 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.4) 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m25F4Vft007666 ==============================End of - Headers==============================
Image-Pro (Windows based) has a function for "Zoom and pan all images simultaneously"; this should do what you're asking for. It's a button in the toolbar next to the Zoom and Pan buttons.
Image-Pro also has tiling, if you want to do your panoramas there.
Kevin Ryan Media Cybernetics, Inc.
-----Original Message----- X-from: amenex-at-amenex.com [mailto:amenex-at-amenex.com] Sent: Wednesday, March 05, 2008 10:12 AM To: Kevin Ryan
The problem at hand is to look very closely at pairs of high-resolution SEM images on the computer monitor. I've managed to piece together two sets of six images each with the hugin open-source panoramic image processing software, but I find that I can see the details in those images only at 100% (pixel-for-pixel) resolution on the monitor of my PC. The process is made more complicated by the need to see small differences in contour on a generally spherical concave surface.
The two montages were made with about a 45 degree difference in viewing angle between the two sets, thereby making a stereo pair. The extreme parallax ought to provide the needed exaggeration in apparent surface relief.
The object under study is a less-than-hemispherical metal socket made with a hemispherical-ended drill. I'm looking at the details of the machining marks in the bowl-shaped socket. Think of looking at your dog's water bowl from just above the rim.
I can open both images in browser (or image-editing) windows that are part-screen (i.e., not maximized) and spaced to match my interpupillary distance, but to pan them I have to switch control from one image to the other in order to pan each image in turn ... that's painfully tedious and distracting.
Is there a way to bring two windows at once under the control of one mouse ? If that were the case, I could view my stereo pairs anywhere within the field of view, right on the monitor, and avoid a lot of cutting and pasting of the original pair of images into a large number of individual pairs that could be placed just 75mm or so apart.
I'm using linux/debian/etch on this PC, but I can use a WinXP laptop in a pinch ...
Thanks, George
George Langford, Sc.D. Principal Consultant Amenex Associates, Inc. amenex-at-amenex.com http://www.amenex.com/
==============================Original Headers============================== 21, 25 -- From kevin-at-mediacy.com Wed Mar 5 09:41:56 2008 21, 25 -- Received: from outbound.exchangemessage.net (outbound.exchangemessage.net [128.241.127.68]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m25Ffuxm021750 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 09:41:56 -0600 21, 25 -- Received: from MCLUS26006MAP.exmsg.net ([128.241.126.171]) by outbound.exchangemessage.net with Microsoft SMTPSVC(6.0.3790.1830); 21, 25 -- Wed, 5 Mar 2008 10:41:55 -0500 21, 25 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 21, 25 -- Content-class: urn:content-classes:message 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="utf-8" 21, 25 -- Subject: Commercial response - RE: [Microscopy] Viewing montages with simultaneous panning 21, 25 -- Date: Wed, 5 Mar 2008 10:42:57 -0500 21, 25 -- Message-ID: {254DA59D1DDEAB47AAC2EFC15FE7A8A1025B35A9-at-MCLUS26006MAP.exmsg.net} 21, 25 -- In-Reply-To: {200803051512.m25FCSJi018773-at-ns.microscopy.com} 21, 25 -- X-MS-Has-Attach: 21, 25 -- X-MS-TNEF-Correlator: 21, 25 -- Thread-Topic: Commercial response - RE: [Microscopy] Viewing montages with simultaneous panning 21, 25 -- Thread-Index: Ach+01Ti/YIKI3LvQOi7MnDqxXMZVQAA8DUg 21, 25 -- References: {200803051512.m25FCSJi018773-at-ns.microscopy.com} 21, 25 -- From: "Kevin Ryan" {kevin-at-mediacy.com} 21, 25 -- To: {Microscopy-at-microscopy.com} 21, 25 -- X-OriginalArrivalTime: 05 Mar 2008 15:41:55.0088 (UTC) FILETIME=[7084D100:01C87ED7] 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id m25Ffuxm021750 ==============================End of - Headers==============================
Hello all, Just about a year ago I have sent following message to listserver: ---------------------------------------------------------------------- We have following trouble: "Panasonic Lithium battery BR-2/3A 3V" which are used for battery back up in Philips CM12 electron microscope were exhausted after one year of use. This occurred for the second time. Is this normal ? Previously we had to replace them after several years but now we have to use the third set of batteries in two years. Has anybody any suggestion or hint? ---------------------------------------------------------------------
I have got several hints, thanks for them, but our trouble still remains. We have checked the expiry date of the batteries, ordered fresh new ones, checked the capacity of new ones before installation, etc. However, the battery lifetime is still about eight months. Any new suggestions? Unfortunately, there is no warning system checking the battery power in Philips CM12 software. The only one message exist that battery back-up failed. But after that, all microscope setting is lost. I am sorry for sending this again, but maybe someone other solved this problem in mean time.
Thanking you in advance. Oldrich
----------------------------------------------- Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 6, 21 -- From benada-at-biomed.cas.cz Wed Mar 5 10:03:35 2008 6, 21 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m25G3Yum002936 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 10:03:35 -0600 6, 21 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 6, 21 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id BB871928010 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 17:03:31 +0100 (CET) 6, 21 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 6, 21 -- Organization: Institute of Microbiology 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Date: Wed, 05 Mar 2008 17:03:32 +0100 6, 21 -- MIME-Version: 1.0 6, 21 -- Subject: Philips CM12 battery back-up trouble, again 6, 21 -- Message-ID: {47CED264.1524.1C825ED-at-benada.biomed.cas.cz} 6, 21 -- Priority: normal 6, 21 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 21 -- Content-type: text/plain; charset=US-ASCII 6, 21 -- Content-transfer-encoding: 7BIT 6, 21 -- Content-description: Mail message body 6, 21 -- X-Antivirus: avast! (VPS 080305-0, 05.03.2008), Outbound message 6, 21 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
We have recently acquired a used Balzers MED 010 coating system with the carbon/metal evaporation head. It still works great, and pumps down to 10^-4 or 10^-5 mbar no problem. We would like to expand its capabilities, and I was wondering if anybody has any old MED 010 parts kicking around? It currently has the sputtering/glow discharge control and the rotary stage control modules installed, but it did not come with a sputtering head or a rotary stage. If anyone has a sputtering/glow discharge head, a rotary stage, and a quartz thickness monitor with thickness monitor module that aren't being used, or knows where we could get these parts, we would be very grateful and would be willing to compensate you for the shipping and parts if necessary.
Thanks in advance, -- Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 6, 29 -- From bnross-at-interchange.ubc.ca Wed Mar 5 12:30:44 2008 6, 29 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m25IUhvV022908 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 12:30:44 -0600 6, 29 -- X-Ubc-Received: from mr6.mail-relay.ubc.ca (localhost [127.0.0.1]) 6, 29 -- by localhost (Postfix) with SMTP id 03C951597A 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 10:30:42 -0800 (PST) 6, 29 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 6, 29 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Mar 2008 10:30:41 -0800 (PST) 6, 29 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14]) 6, 29 -- by smtp.interchange.ubc.ca 6, 29 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 6, 29 -- with ESMTP id {0JX9006ISSR54F-at-smtp.interchange.ubc.ca} for 6, 29 -- Microscopy-at-microscopy.com; Wed, 05 Mar 2008 10:30:41 -0800 (PST) 6, 29 -- Date: Wed, 05 Mar 2008 10:30:41 -0800 (PST) 6, 29 -- From: Bradford Ross {bnross-at-interchange.ubc.ca} 6, 29 -- Subject: Coating: Surplus MED 010 Parts? 6, 29 -- To: Microscopy-at-microscopy.com 6, 29 -- Message-id: {5799715.3421204741841250.JavaMail.myubc2-at-handel.my.ubc.ca} 6, 29 -- MIME-version: 1.0 6, 29 -- X-Mailer: uPortal WEB email client 3.0 6, 29 -- Content-type: text/plain; charset=us-ascii 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.5.101256 6, 29 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 6, 29 -- X-PerlMx-Spam: Probability=7%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_900_999 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 6, 29 -- X-Spam-Level: 6, 29 -- X-Spam-Flag: No ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both james.romanow-at-uconn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: james.romanow-at-uconn.edu Name: Jim Romanow
Organization: The University of Connecticut
Title-Subject: [Filtered] Digital Camera for Olympus PhotoMax Light Scope?
Question: Hello everyone,
I would like to modify my 1960s Olympus Photomax light microscope to accept a digital camera or camera back. The images will mainly be used for evaluating stained thick epoxy sections. I have removed the shutter from the photo unit but left all of the lenses in place. At present the original Olympus PA-35, 35mm camera body, is attached with a bayonet mount. After reading information from many websites I realize that there are several kinds of bayonet mounts and adaptors as well as many camera options so I thought it best to ask for opinions and recommendations. Thank you in advance.
Best Regards, Jim
James S. Romanow The University of Connecticut Electron Microscopy Laboratory BSP Building Room G06, Unit 3242 91 North Eagleville Road Storrs, CT 06269-3242
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ramshes-at-musc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ramshes-at-musc.edu Name: Venkat Ramshesh
Organization: Medical Univeristy of South Carolina
Title-Subject: [Filtered] Second Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Question: Second Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB) Medical University of South Carolina May 18-23, 2008
The Second Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB) Workshop provides a solid introduction to the concepts and practical applications of light microscopy relevant to modern cell and molecular biology. Students will have opportunities for extensive hands-on experience with state-of-the-art equipment for optical imaging, digital image processing, and fluorescence and confocal/multiphoton microscopy guided by experienced academic and commercial faculty. Lectures and laboratory exercises will include: optics of image formation; microscope alignment; phase contrast and differential interference contrast microscopy; video and digital cameras; contrast enhancement by analog and digital image processing; principles of fluorescence and fluorescence microscopy; ion imaging and fluorescent probes, including green fluorescent protein; fluorescence resonance energy transfer; and laser scanning confocal and multiphoton microscopy. A commercial faculty representing leading microscope manufacturers will make available for student use the latest and most advanced instrumentation for light microscopy, image detection and computerized image analysis. The LMB Workshop is designed for doctoral level scientists, advanced pre-doctoral students and high level technical personnel. No prior experience with microscopy is required. All students will benefit from in-depth interaction with instructors. Students are encouraged to bring their own specimens for analysis.
Tuition: $750.00 Application Deadline: April 1, 2008 Principal Instructors: John J. Lemasters, M.D., Ph.D., Organizer P. Darwin Bell, Ph.D. Margaret Kelly, Ph.D. Peter Komlosi, Ph.D. Anna-Liisa Nieminen, Ph.D. Venkat Ramshesh, Ph.D.
To apply, send a curriculum vita and a brief letter describing your research interests and reasons for enrolling. Because the course is expected to be oversubscribed, applicants should inquire as soon as possible. Please indicate your complete mailing address, telephone/fax number and email address. Full consideration will be given to applications received by April 1, 2008.
For further information or to apply, contact: Venkat K Ramshesh Medical University of South Carolina Center for Cell Death, Injury and Regeneration and Hollings Cancer Center 280 Calhoun Street, PO Box 250140 Charleston, SC 29425 Telephone (843) 792- 3530, FAX (843) 792-1617 E-mail: ramshes-at-musc.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both atm2-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: atm2-at-psu.edu Name: Arthur Motta
Organization: Penn state University
Title-Subject: [Microscopy] Faculty Position in Materials Engineering
Question: Department of Mechanical and Materials Engineering
Tenure Track Faculty Position in Materials Engineering
The Department of Mechanical and Materials Engineering in the Faculty of Applied Science, Queenís University, invites applications for a tenure track faculty position at the Assistant Professor level in materials engineering, commencing January 1, 2009, subject to the availability of the chosen candidate. Queenís University, NSERC and the Canadian nuclear industry have established an internationally recognized Industrial Research Chair (IRC) program in Nuclear Materials. The chair program is now in its second five year term, with current funding of ~4M$. The senior Chair-holder works in the areas of radiation damage, radiation-induced deformation and properties of zirconium and nickel alloys in nuclear applications. The associate Chair-holder, who also holds a Canada Research Chair (CRC) in Mechanics of Materials, works in the areas of multi-scale modeling, mechanics of materials and materials characterization by neutron and X-ray diffraction, with a special emphasis on nuclear materials. The successful candidate will work closely with the Chair-holders, and will perform research independent but complementary to the chair program. One possible research area is materials characterization by electron-optical techniques and microstructure-property relationships. Further information on the chair program can be found at http://me.queensu.ca/research/nuclear/. Applicants must hold a Ph.D. degree in Materials and/or Metallurgical Engineering (or be near completion) and will have expertise in an area that supports the chair program. The successful candidate will be required to teach graduate and undergraduate courses and contribute to the development of curriculum, supervise graduate students, develop a vigorous research program, interact with industry (in particular the industrial sponsors of the Chair program) and make administrative contributions through service to the University, Faculty and Department. Registration as a Professional Engineer of Ontario, or eligibility to acquire registration in Canada, is an essential qualification. The Department of Mechanical and Materials Engineering currently has an enrolment of about 480 undergraduate students and over 100 graduate students in the M.Sc. (Eng.) and Ph.D. programs. Mechanical and Materials Engineering is an active partner in the Integrated Learning Centre (ILC). In addition to the NSERC IRC/CRC program in Nuclear Materials, research is supported by an NSERC Design Chair, a CRC in Computational Turbulence, a CRC in Tissue Engineering and a QRC in Fluid Dynamics and Multi-scale Phenomena. The Centre for Advanced Materials and Manufacturing (CAMM), the Centre for Manufacturing of Advanced Ceramics and Nanomaterials (CMACN), the Fuel Cell Research Centre (FCRC), the Human Mobility Research Centre (HMRC), the High Performance Computing Virtual Laboratory (HPCVL), and other initiatives, further support research in the department and the faculty. Further information on the department can be found at: http://me.queensu.ca/. Applicants should send their curriculum vitae, an e-mail address, the names and the mailing addresses of three referees, a statement of teaching and research interests, and three examples of relevant publications to: Chair, Appointments Committee Department of Mechanical and Materials Engineering, Queenís University McLaughlin Hall, 130 Stuart Street Kingston, Ontario K7L 3N6 FAX: (613) 533-6489 Review of applications will begin on May 1, 2008. Applications will be accepted until the position is filled. The University invites applications from all qualified individuals. Queenís is committed to employment equity and diversity in the workplace and welcomes applications from women, visible minorities, aboriginal people, persons with disabilities, and persons of any sexual orientation or gender identity.
Please do not take the test. It was sent to me by my aunt and when you take the test and click on view results it send it to every one in your address book. I sincerely apologize for that mistake.
Please do not take the test. It was sent to me by my aunt and when you take the test and click on view results it send it to every one in your address book. I sincerely apologize for that mistake.
We have had a request to HPF-FS drosophila eyes for ICC. This is new for us so I would really appreciate some help.
For normal microwave fixation we just cut the head in half and process using Glut in cacodyate with salts with embedding in EPON generic. This works quite well.
We will want to embed in HM-20 for the HPF eyes at low temperature. Can we effectively freeze whole eyes? What filler would you recommend, what substitution media, and what schedule for the FS and infiltration? Any tips on the embedding? They will have to be oriented but we may have to do that after polymerization.
Thanks, Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 7, 21 -- From dsherman-at-purdue.edu Thu Mar 6 10:37:56 2008 7, 21 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m26GbuoQ018525 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 6 Mar 2008 10:37:56 -0600 7, 21 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 21 -- Thu, 6 Mar 2008 11:37:56 -0500 7, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 7, 21 -- Thu, 6 Mar 2008 16:37:55 +0000 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 7, 21 -- Date: Thu, 06 Mar 2008 11:37:55 -0500 7, 21 -- Subject: HPF-Drosophila eyes 7, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C3F58813.2958A%dsherman-at-purdue.edu} 7, 21 -- Thread-Topic: HPF-Drosophila eyes 7, 21 -- Thread-Index: Ach/qG2Yq+waGeubEdyRDgARJN08Mg== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-OriginalArrivalTime: 06 Mar 2008 16:37:56.0164 (UTC) FILETIME=[6E4A1040:01C87FA8] ==============================End of - Headers==============================
We are in the process of upgrading our light microscope imaging system and I want to be sure I understand the parameters of camera selection correctly as far as resolution goes. Most of our high mag work is fluorescence and most of the low mag work is color. It seems since the CCD is positioned in the same focal plane, the resolution is controlled by the pixel size because it directly relates to the Nyquist sampling rate. The number of pixels or size of the array relates to the field of view or the area of your sample that can be recorded. Is this correct?
I have noted that with a pixel size of 6.8µm on our system the sampling rate goes from 2.5 using a 60x objective (good), down to to 1.5 using a 10x objective (bad). It seems you are penalized for having high quality low magnification lenses. Do I need two different cameras? One camera for high mag fluorescence and a different camera for low mag color? In order to get a 2.5 sampling rate for low magnification work do I need a pixel shifting camera or can you put an SLR camera back that seems to have 5 to 10 megapixel arrays for a lot cheaper (but what is the pixel size)?
The last question: I have noticed that some CCDs have 6.45µm pixel sizes which seems very good but they are interline CCDs. Does this mean they are spatially acting like 12.9µm pixels if every other line is being used?
Thanks for any comments
Robert Underwood University of Washington Dermatology
==============================Original Headers============================== 13, 23 -- From underwoo-at-u.washington.edu Thu Mar 6 14:52:15 2008 13, 23 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m26KqEJO009765 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Mar 2008 14:52:14 -0600 13, 23 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103]) 13, 23 -- by mxout4.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m26KqD8M007672 13, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Mar 2008 12:52:13 -0800 13, 23 -- Received: from localhost (localhost [127.0.0.1]) 13, 23 -- by hymn13.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m26KqDuo015418 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Mar 2008 12:52:13 -0800 13, 23 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Thu, 06 Mar 2008 12:52:13 PST 13, 23 -- Date: Thu, 6 Mar 2008 12:52:13 -0800 (PST) 13, 23 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 13, 23 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 13, 23 -- Subject: [Microscopy] CCD Pixel size / resolution 13, 23 -- Message-ID: {Pine.LNX.4.43.0803061252130.26971-at-hymn13.u.washington.edu} 13, 23 -- MIME-Version: 1.0 13, 23 -- Content-Type: TEXT/PLAIN; charset=ISO-8859-1; format=flowed 13, 23 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.6.123413 13, 23 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='SUPERLONG_LINE 0.05, __CP_MEDIA_2_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id m26KqEJO009765 ==============================End of - Headers==============================
Might anyone out there have a JEOL GS stage that they no longer want?
I'd also be interested in hearing from anyone with a non functioning 733. I'd like to acquire one to use for spare parts.
Cheers Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 11, 18 -- From mmcheath-at-syr.edu Thu Mar 6 15:58:55 2008 11, 18 -- Received: from SUEXCL-02.ad.syr.edu (suexbe-02.ad.syr.edu [128.230.108.46]) 11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m26Lwr2q024825 11, 18 -- for {Microscopy-at-Microscopy.Com} ; Thu, 6 Mar 2008 15:58:54 -0600 11, 18 -- Received: from 24.59.96.168 ([24.59.96.168]) by SUEXCL-02.ad.syr.edu ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu ([128.230.108.49]) with Microsoft Exchange Server HTTP-DAV ; 11, 18 -- Thu, 6 Mar 2008 21:58:52 +0000 11, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 11, 18 -- Date: Thu, 06 Mar 2008 16:58:51 -0500 11, 18 -- Subject: Looking for JEOL GS stage 11, 18 -- From: Michael Cheatham {mmcheath-at-syr.edu} 11, 18 -- To: {Microscopy-at-Microscopy.Com} 11, 18 -- Message-ID: {C3F5D34B.1B5AD%mmcheath-at-syr.edu} 11, 18 -- Thread-Topic: Looking for JEOL GS stage 11, 18 -- Thread-Index: Ach/1UMQgZJYfuvIEdykPAAWy6AryQ== 11, 18 -- Mime-version: 1.0 11, 18 -- Content-type: text/plain; 11, 18 -- charset="US-ASCII" 11, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Problem solved in this case. We did measurements with one of my colleague technician from the electronics department and made a straight pin to pin cable, and now the camera works!
Cheers!
Laci
==============================Original Headers============================== 4, 29 -- From jakabfarkaslaszlo-at-gmail.com Thu Mar 6 16:24:24 2008 4, 29 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.191]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m26MOOv8005963 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Mar 2008 16:24:24 -0600 4, 29 -- Received: by rv-out-0910.google.com with SMTP id k20so32108rvb.30 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 06 Mar 2008 14:24:23 -0800 (PST) 4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 29 -- d=gmail.com; s=gamma; 4, 29 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 4, 29 -- bh=P7lLtyb9r5GcSPQsOqY/w1TZhL+3LWuCfg9h+ci2d8Y=; 4, 29 -- b=KDec8zlgWswNnzyi6mlvQ2Bqcae7MhmmHpA5SkH1r0hbywxFzKMbzftL0sA9Er7wVm7pbITvy3vrgBFQmfgXl/jqlmORJ/8ulTng3kAhUV/uT5laPguTIBfc7RvMNcamJy+b7ubv01vgDG+dEm2zy9ra96tR+yt+uZdi30jrwOg= 4, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 29 -- d=gmail.com; s=gamma; 4, 29 -- h=message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 4, 29 -- b=mYez81pbsJ8bPmviknrhosNsX4ZYXcnT0u3VcviVYI/cZSEXVQWJZiFgwyFfO8NVFVaf2TEIeta7+k0bJw81RjIs3ZO7FchrkgTfJiW28vAGhjDQTOAnQVqYa3TfKj/MHRkJrg6SpZVXaPp4v/DY7+p5WbuJ+OqIYbomRQzP4Ms= 4, 29 -- Received: by 10.140.88.11 with SMTP id l11mr244354rvb.74.1204842263821; 4, 29 -- Thu, 06 Mar 2008 14:24:23 -0800 (PST) 4, 29 -- Received: by 10.141.88.8 with HTTP; Thu, 6 Mar 2008 14:24:23 -0800 (PST) 4, 29 -- Message-ID: {a3da032b0803061424l3271aa35kab01cd2363843b47-at-mail.gmail.com} 4, 29 -- Date: Fri, 7 Mar 2008 00:24:23 +0200 4, 29 -- From: "=?ISO-8859-1?Q?Laszl=F3_Jakab-Farkas?=" {jflaci-at-ms.sapientia.ro} 4, 29 -- Sender: jakabfarkaslaszlo-at-gmail.com 4, 29 -- To: Microscopy-at-microscopy.com 4, 29 -- Subject: [Microscopy] Missing cable, could somebody help? (Solved) 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset=ISO-8859-1 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- Content-Disposition: inline 4, 29 -- X-Google-Sender-Auth: 592fc94ab37fb1bd ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both randy-nessler-at-uiowa.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: randy-nessler-at-uiowa.edu Name: Randy Nessler
Organization: University of Iowa
Title-Subject: [Filtered] Mouse eye TEM processing
Question: I have a project where they want, no, almost demand that I process a whole mouse eye for TEM thin sectioning. I've had mixed success in the past with extended infiltrations and was wondering if anybody had a pet protocol they would be willing to share. TIA
This Question was submitted to Ask-A-Microscopist by (exploratorium-at-tiscali.it) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, March 6, 2008 at 12:51:04 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both exploratorium-at-tiscali.it as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: exploratorium-at-tiscali.it Name: giovanni de caro
Organization: museo luigi montalbÚ - casalciprano . italia
Education: 9-12th Grade High School
Location: Casalciprano, Italia
Question: We are in bad need of the english operating manual of the AMR 1000 scanning electron microscope. Anyone of you can provide us a photocopy of it? Thank you
(PS: to learn more about our activities pleas visit our website: http://web.tiscali.it/exploratorium)
A hi-sensitivity fluorescence camera [e.g. Hammamatsu Orca ER] is only a megapixel or so, but it scores by being very low noise and very sensitive - hence people pay it's £10k price tag. It can also do things like binning pixels for higher sensitivity with low noise. Plus it's only B&W so no red/green/ blue pixels and bayer mosaic processing to worry about. My Olympus SLR might have a 10 mega-pixel higher resolution but that's not what required for rapid low noise picture capture of fluorescence specimens. You can increase resolution by changing objective and optical zoom.
Similarly for colour capture you need a decent microscope colour camera rather video camera, and then you have to get into VDU colour correction, transmission lamp bulb spectral response, white balance correction, etc... - all a bit too much to go into on the list-server. These are a lot cheaper than B&W fluorescence cameras, and have many more pixels (although they are spilt three ways colour wise).
Generally I just call up our local wide-field microscope rep for that system, and select one of their recommended cameras, as they are pretty clued up on this area and if you run into problems, one manufacturer can't blame the other for any resulting problems (which can run on for years). If they don't know what works best with their microscopes they would be out of business these days.
If you aren't that bothered about absolute image quality, or quantifying image intensities and areas, then a standard prosumer colour camera attached to a microscope can be a cheap alternative for pretty pictures of bright-field samples - again manufacturers have recommended digital cameras and port adapters. But this isn't good enough for [monochrome] fluorescence images. There are many second party microscope camera suppliers that may undercut microscope manufacturers prices, but given the high cost of microscope imaging and sample preparation, I would make sure that I don't fall at the last hurdle.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: underwoo-at-u.washington.edu [mailto:underwoo-at-u.washington.edu] Sent: 06 March 2008 21:04 To: kjmorris-at-well.ox.ac.uk
Hello Fellow Microscopists,
We are in the process of upgrading our light microscope imaging system and I want to be sure I understand the parameters of camera selection correctly as far as resolution goes. Most of our high mag work is fluorescence and most of the low mag work is color. It seems since the CCD is positioned in the same focal plane, the resolution is controlled by the pixel size because it directly relates to the Nyquist sampling rate. The number of pixels or size of the array relates to the field of view or the area of your sample that can be recorded. Is this correct?
I have noted that with a pixel size of 6.8µm on our system the sampling rate goes from 2.5 using a 60x objective (good), down to to 1.5 using a 10x objective (bad). It seems you are penalized for having high quality low magnification lenses. Do I need two different cameras? One camera for high mag fluorescence and a different camera for low mag color? In order to get a 2.5 sampling rate for low magnification work do I need a pixel shifting camera or can you put an SLR camera back that seems to have 5 to 10 megapixel arrays for a lot cheaper (but what is the pixel size)?
The last question: I have noticed that some CCDs have 6.45µm pixel sizes which seems very good but they are interline CCDs. Does this mean they are spatially acting like 12.9µm pixels if every other line is being used?
Thanks for any comments
Robert Underwood University of Washington Dermatology
==============================Original Headers============================== 13, 23 -- From underwoo-at-u.washington.edu Thu Mar 6 14:52:15 2008 13, 23 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m26KqEJO009765 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Mar 2008 14:52:14 -0600 13, 23 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103]) 13, 23 -- by mxout4.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m26KqD8M007672 13, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Mar 2008 12:52:13 -0800 13, 23 -- Received: from localhost (localhost [127.0.0.1]) 13, 23 -- by hymn13.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m26KqDuo015418 13, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 6 Mar 2008 12:52:13 -0800 13, 23 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Thu, 06 Mar 2008 12:52:13 PST 13, 23 -- Date: Thu, 6 Mar 2008 12:52:13 -0800 (PST) 13, 23 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 13, 23 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 13, 23 -- Subject: [Microscopy] CCD Pixel size / resolution 13, 23 -- Message-ID: {Pine.LNX.4.43.0803061252130.26971-at-hymn13.u.washington.edu} 13, 23 -- MIME-Version: 1.0 13, 23 -- Content-Type: TEXT/PLAIN; charset=ISO-8859-1; format=flowed 13, 23 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.6.123413 13, 23 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='SUPERLONG_LINE 0.05, __CP_MEDIA_2_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id m26KqEJO009765 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 21 -- From kjmorris-at-well.ox.ac.uk Fri Mar 7 04:25:04 2008 27, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 27, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m27AP46t005139 27, 21 -- for {Microscopy-at-Microscopy.Com} ; Fri, 7 Mar 2008 04:25:04 -0600 27, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 27, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 27, 21 -- id 1JXZl9-0007v1-HZ 27, 21 -- for Microscopy-at-Microscopy.Com; Fri, 07 Mar 2008 10:25:03 +0000 27, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 27, 21 -- To: {Microscopy-at-Microscopy.Com} 27, 21 -- Subject: FW: [Microscopy] CCD Pixel size / resolution 27, 21 -- Date: Fri, 7 Mar 2008 10:25:52 -0000 27, 21 -- Message-ID: {000701c8803d$9e8c14c0$b22c4381-at-CytoWhizz} 27, 21 -- MIME-Version: 1.0 27, 21 -- Content-Type: text/plain; 27, 21 -- charset="iso-8859-1" 27, 21 -- X-Mailer: Microsoft Office Outlook 11 27, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 27, 21 -- Thread-Index: Ach/zZXmKjtJ/C3SQIq2NT9ZpThaaQAbGWogAADkXiA= 27, 21 -- Content-Transfer-Encoding: 8bit 27, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m27AP46t005139 ==============================End of - Headers==============================
Here is Tech Extraordinaire Chery Jensen's protocol for whole mouse eyes. For those without microwaves, you may need to experiment with times, but certainly fixation, etc., times should be longer than your standard mouse liver times. Cheryl recommends at least 1-2 hours in the osmium step. The extended infiltration times may mean that the microwave is overkill during infiltration, but better safe than sorry?
Note: the 2-ME buffer refers to 2-mercaptoethanol added to the buffer as a way of preventing osmium precipitation. We use this routinely now, due to a two-year period of fighting pepper contamination that popped up one day and refused to leave our lab. The 2-ME solved the problem completely, if odiferously. We have formulas, protocols, etc., on our website at http://www.emc.missouri.edu/pandp.htm.
Feel free to contact us with questions.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
CHERYL'S TEM PROTOCOL FOR WHOLE EYES
Primary fixation: MW 120W - 1min off, 80sec on, 3min off, & 40sec on at 350W PI (Katz/Sharma) Fixative Composition for optic nerve and retina 2.0% glutaraldehyde; 1.12% paraformaldehyde; 0.13 M Na-cacodylate; 0.13 mM CaCl2; pH 7.40
DAY 1 1). Buffer Rinses: 2-ME buffer - 3 rinses x 20min -at- on rocker. (0.01M 2-mercaptoethanol, 0.1M Na Cacodylate buffer, 0.17M sucrose)
2). Secondary fixation: 1% OsO4 (ex.300uL 2-ME:100uL4% OsO4) Microwave 120W under vacuum, on Coldspot (recirculating water bath---Ted Pella) - program: 1min off, 80 second irradiation, 3min off, 40 second irradiation.
3). Buffer Wash: 3 quick (on/off) rinses with 2-ME buffer then 2 x 5min
4). Milli-Q Water Wash: 1 quick (on/off) then 3x10min Milli-Q water rinses
5). Dehydration EtOH: Microwave 120W, under vacuum, on coldspot - 40 second irradiation each: 20%, 50%, 70%, 90%, 95% 4x100%EtOH
6). Resin Infiltration with LR White: Caution: do not use "old" LRW resin as it tends to polymerize during the infiltration steps! Microwave 250W, 3min each under vacuum on cold spot 2EtOH:1 LRW after MW place on rocker for approx 2hr; 1EtOH:1LRW after MW place on rocker overnight.
DAY 2 Continue Resin Infiltration: Early Morning: 1EtOH:2LRW after MW place on rocker Between 4 - 5pm 1st pure LRW resin exchange - after MW place on rocker overnight
DAY 3 Continue Resin Infiltration: Early Morning: 2nd pure LRW resin exchange - after MW place on rocker Between 4 - 5pm 3rd pure LRW resin exchange - after MW place on rocker overnight
DAY 4 Continue Resin Infiltration: Early Morning: 4th pure LRW resin exchange - after MW place on rocker Between 4 - 5pm 5th pure LRW resin exchange - after MW place on rocker overnight
Continue Resin Infiltration: Early Morning: 6th pure LRW resin exchange - after MW place on rocker
7). Embedding and Polymerization: 4pmish flat embed samples and polymerize for 24hr at 60C.
-----Original Message----- X-from: randy-nessler-at-uiowa.edu [mailto:randy-nessler-at-uiowa.edu] Sent: Thursday, March 06, 2008 6:36 PM To: Tindall, Randy D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both randy-nessler-at-uiowa.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: randy-nessler-at-uiowa.edu Name: Randy Nessler
Organization: University of Iowa
Title-Subject: [Filtered] Mouse eye TEM processing
Question: I have a project where they want, no, almost demand that I process a whole mouse eye for TEM thin sectioning. I've had mixed success in the past with extended infiltrations and was wondering if anybody had a pet protocol they would be willing to share. TIA
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Title-Subject: [Filtered] immunogold staining of DNA and/or chitosan
Question: Hello, Does there is someone that know if one can perform immunogold staining for DNA and chitosan polymer or if there are other methods for staining DNA and chitosan in electron microscopy?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hgabrisc-at-uno.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hgabrisc-at-uno.edu Name: Heike Gabrisch
Organization: University of New Orleans
Title-Subject: [Filtered] Desktop Microscopist
Question: I am trying to get in touch with Lacuna labs to order an extra key for Desktop Microscopist (purchased in 2002) but couldn't find a contact on the web. Does anyone have a contact address ?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (caesar.buie-at-gmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, March 7, 2008 at 13:07:07 ---------------------------------------------------------------------------
Email: caesar.buie-at-gmail.com Name: Caesar
Organization: OSU
Education: Undergraduate College
Location: columbus, ohio, usa
Question: I plan to learn how to prepare TEM samples from NiTi wires.
Hi all, I would like to do ellipsometry in a HF containing solution and I need your suggestions in what material I can use for the windows as an alternative to quartz.
Thanks in advance for any advice or helpful hints.
Hany
-- ********************************************************** Hany El-Sayed Graduate student Chemistry department University of Calgary, Alberta Canada 403-220-7306 x: 26322 helsayed-at-ucalgary.ca **********************************************************
==============================Original Headers============================== 5, 27 -- From ramadanhany-at-gmail.com Sat Mar 8 00:51:35 2008 5, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m286pYUL025194 5, 27 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2008 00:51:34 -0600 5, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so561932rvb.30 5, 27 -- for {microscopy-at-microscopy.com} ; Fri, 07 Mar 2008 22:51:34 -0800 (PST) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=/i9P3uiGZpZKsPfnLtMCfwKGRzmJ3zDHHPhHQZgIZds=; 5, 27 -- b=ksTZsnKopzFiYdMpQygVWH8bIrzSVRrXNvDU+Sb5dWTk4hBqe0LWW+QzKaXarlA3HKo5cq8iCb4k2NX+nwn9jSmN2/HWw8Ai6Cy6NoInEY8dNln2rmhqwQBjWZ0tUAE9WmU9r/0ZQHePOW1lIn/e+VTZxtsAjn1LMMBCgbJaLxY= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=EuoK6JbEcju22wFAGBcIEQdc+ScPohhlDXS6oM9X5fHncvpGIYo8VNxnA2vf+XRpvqoHt/pGJ1cpfruslynVWIpeysV0lzXojMKOqEwTnThviiujL8ZGqPTDzLgq3O/GVUa9jHDUIsBbjnxy47KemqBHTiB3PXnPCpkcWOk7ln8= 5, 27 -- Received: by 10.141.89.13 with SMTP id r13mr1478346rvl.177.1204959094352; 5, 27 -- Fri, 07 Mar 2008 22:51:34 -0800 (PST) 5, 27 -- Received: by 10.141.71.2 with HTTP; Fri, 7 Mar 2008 22:51:34 -0800 (PST) 5, 27 -- Message-ID: {8d8ce5a30803072251y73a04e91p89f6549a5142222b-at-mail.gmail.com} 5, 27 -- Date: Fri, 7 Mar 2008 23:51:34 -0700 5, 27 -- From: "Hany Ramadan" {ramadanhany-at-gmail.com} 5, 27 -- To: microscopy {microscopy-at-microscopy.com} 5, 27 -- Subject: Ellipsometry in HF solutions ..... any alternative to quartz windows? 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I'm trying to locate an affordable unit to (probably) plasma clean coated SEM pin stub specimens. This is to get rid of the HC contamination rectangles. The goal is to clean the specimen without damaging it. The specimen could have been coated with nothing, Pd, Au/Pd, Ir or Pt. Re-coating is OK. The specimens can be bio or metallurgical.
It seems that a small table top unit with low energy Ar plasma would do the job. My coater will not etch (Denton Desk IV TSC).
I don't think the SEM chamber cleaners are of use in my application since all pumps are dry scroll types. Chamber vent is not done since a load lock is used. Omnipresent C does not seem to be an issue in the SEM. It seems to be more towards specimens that are in atmosphere too long before going into vacuum storage.
Commercial feedback is welcomed off-line.
tnx, gary g.
==============================Original Headers============================== 7, 17 -- From gary-at-gaugler.com Sat Mar 8 15:35:58 2008 7, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m28LZwIs004166 7, 17 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2008 15:35:58 -0600 7, 17 -- Message-Id: {200803082135.m28LZwIs004166-at-ns.microscopy.com} 7, 17 -- Received: (qmail 9605 invoked from network); 8 Mar 2008 13:35:57 -0800 7, 17 -- Received: by simscan 1.1.0 ppid: 9602, pid: 9603, t: 0.1269s 7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 17 -- by smtp2 with SMTP; 8 Mar 2008 13:35:57 -0800 7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 17 -- Date: Sat, 08 Mar 2008 13:35:54 -0800 7, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 7, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 17 -- Subject: Specimen cleaning unit 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-48434BA ==============================End of - Headers==============================
Quite recently I had very positive experience with removing hydrocarbon contamination from semiconductor samples prior to backside FIB work by UV/ozone cleaning; about 10 years ago we also used UV generated ozone to remove HC contamination from CD-SEM test wafers (very similar to what you are trying to do).
Desktop device I used for FIB samples was made by Jelight (www.jelight.com), you can get a second-hand unit for about 5.5K$ (www.bidservice.com), or even make your own UV Ozone cleaner for under 1K$ (contact me offline for the details). Both room-air supply and CDA seemed to work just fine with Jelight cleaner; you can try to bleed low-pressure O2 through needle valve, or better yet to substitute air supply by Nitrox32 or Nitrox36 mix made for SCUBA diving (32% or 36% O2 enriched air) to speed up the cleaning. Advantage of using Nitrox is that the mix is controlled and much safer to handle then pure O2; get Nitrox from certified "oxygen clean" facility.
Hope this info is helpful and I have no association with mentioned vendors.
Cheers,
Valery Ray ========================= www.partbeamsystech.com
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Hi Listers:
I'm trying to locate an affordable unit to (probably) plasma clean coated SEM pin stub specimens. This is to get rid of the HC contamination rectangles. The goal is to clean the specimen without damaging it. The specimen could have been coated with nothing, Pd, Au/Pd, Ir or Pt. Re-coating is OK. The specimens can be bio or metallurgical.
It seems that a small table top unit with low energy Ar plasma would do the job. My coater will not etch (Denton Desk IV TSC).
I don't think the SEM chamber cleaners are of use in my application since all pumps are dry scroll types. Chamber vent is not done since a load lock is used. Omnipresent C does not seem to be an issue in the SEM. It seems to be more towards specimens that are in atmosphere too long before going into vacuum storage.
Commercial feedback is welcomed off-line.
tnx, gary g.
==============================Original Headers============================== 7, 17 -- From gary-at-gaugler.com Sat Mar 8 15:35:58 2008 7, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m28LZwIs004166 7, 17 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2008 15:35:58 -0600 7, 17 -- Message-Id: {200803082135.m28LZwIs004166-at-ns.microscopy.com} 7, 17 -- Received: (qmail 9605 invoked from network); 8 Mar 2008 13:35:57 -0800 7, 17 -- Received: by simscan 1.1.0 ppid: 9602, pid: 9603, t: 0.1269s 7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 17 -- by smtp2 with SMTP; 8 Mar 2008 13:35:57 -0800 7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 17 -- Date: Sat, 08 Mar 2008 13:35:54 -0800 7, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 7, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 17 -- Subject: Specimen cleaning unit 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-48434BA ==============================End of - Headers==============================
==============================Original Headers============================== 19, 30 -- From vray-at-partbeamsystech.com Sat Mar 8 21:50:40 2008 19, 30 -- Received: from n3a.bullet.mail.ac4.yahoo.com (n3a.bullet.mail.ac4.yahoo.com [76.13.13.66]) 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m293odJp029315 19, 30 -- for {microscopy-at-microscopy.com} ; Sat, 8 Mar 2008 21:50:39 -0600 19, 30 -- Received: from [76.13.13.25] by n3.bullet.mail.ac4.yahoo.com with NNFMP; 08 Mar 2008 19:49:39 -0000 19, 30 -- Received: from [68.142.237.88] by t4.bullet.mail.ac4.yahoo.com with NNFMP; 09 Mar 2008 03:52:04 -0000 19, 30 -- Received: from [216.252.111.166] by t4.bullet.re3.yahoo.com with NNFMP; 09 Mar 2008 03:52:04 -0000 19, 30 -- Received: from [127.0.0.1] by omp101.mail.re3.yahoo.com with NNFMP; 09 Mar 2008 03:52:04 -0000 19, 30 -- X-Yahoo-Newman-Id: 281519.62855.bm-at-omp101.mail.re3.yahoo.com 19, 30 -- Message-ID: {281519.62855.bm-at-omp101.mail.re3.yahoo.com} 19, 30 -- Received: (qmail 39026 invoked from network); 9 Mar 2008 03:52:04 -0000 19, 30 -- Received: from unknown (HELO cp1198275a) (partbeamsystech-at-75.67.53.103 with login) 19, 30 -- by smtp103.plus.mail.re1.yahoo.com with SMTP; 9 Mar 2008 03:52:03 -0000 19, 30 -- X-YMail-OSG: 6t3rTC4VM1nIcl3ESNbBfhXVSzWCqw2dax5ZEWgXILSN2fswcgX5TI.PDVmYbJNfHyakKsPlAGCVyN7S5bkBlQrOBvUgjtNW09TGpX7uUr4L.U0h3z7HHzUg2RfyJ1213dJHz93nn5Elmdi.rbUhCHfoUHhYa8MfX.Ixb_9YvLdwVG8ivOOiVeLbM9lh8RkE3.8BzzZ_g0QRsw-- 19, 30 -- X-Yahoo-Newman-Property: ymail-3 19, 30 -- Reply-To: {vray-at-partbeamsystech.com} 19, 30 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 19, 30 -- To: {gary-at-gaugler.com} 19, 30 -- Cc: {microscopy-at-microscopy.com} 19, 30 -- Subject: RE: [Microscopy] Specimen cleaning unit 19, 30 -- Date: Sat, 8 Mar 2008 22:54:26 -0500 19, 30 -- Organization: PBST / MEO Engineering 19, 30 -- MIME-Version: 1.0 19, 30 -- Content-Type: text/plain; 19, 30 -- charset="us-ascii" 19, 30 -- Content-Transfer-Encoding: 7bit 19, 30 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 19, 30 -- Thread-Index: AciBcjs6dYbVprEJTrON6L57TT0/5wAF6YRw 19, 30 -- In-Reply-To: {200803082137.m28LbQfP005874-at-ns.microscopy.com} 19, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I have someone who is interested in whether Methane Hydrate has, or can be, studied by either SEM or TEM. He is taking a Geology class where this came up and he came to me for information. I am totally unfamiliar with clathrates so I turn to the wisdom on the listserve for help. From a quick look on the web it looks like it would have to be a cryo technique. Has or is anyone out there done/doing EM on these?
Thanks for any information,
Thomas M. Murray, Ph.D. Senior Research Support Specialist / Instructor College of Nanoscale Science & Engineering University at Albany-SUNY 251 Fuller Rd. Albany, NY 12203
Colloidal gold has been used for the detection of DNA, but bear in mind that the size of the probe (gold particle and whatever other macromolecules are attached to it) is significantly greater than the size of the DNA molecule, therefore precise localization is difficult, if not impractical.
Another method for imaging DNA is platinum shadowcasting.
Daryl Meyer
----- Original Message ----- X-from: monica.nelea-at-polymtl.ca
Hi listers
I am going to be doing some TEM-audoradiography. I have done lots of audorad for light microscopy, but this is the first time I am trying TEM stuff. I have read a bunch of papers and think I know what I am doing, but:
1) do people have suggestions for photographic emulsion and developer? 2) where should I get the above? 3) does anyone have a good protocol?
Thank you all David
==============================Original Headers============================== 6, 20 -- From Elliott-at-arizona.edu Mon Mar 10 11:58:59 2008 6, 20 -- Received: from smtpgate.email.arizona.edu (frodo.email.arizona.edu [128.196.133.168]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2AGwwXw004200 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Mar 2008 11:58:58 -0500 6, 20 -- Received: from frodos_amavis (amavis2.email.arizona.edu [10.0.0.205]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 609CD2E9890 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Mar 2008 09:58:58 -0700 (MST) 6, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 1EE212E9795 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 10 Mar 2008 09:58:57 -0700 (MST) 6, 20 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- Message-Id: {6A9EF29A-A589-4EFE-B150-F01DDCD434A3-at-arizona.edu} 6, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- From: David Elliott {Elliott-at-arizona.edu} 6, 20 -- Subject: TEM-audorad question 6, 20 -- Date: Mon, 10 Mar 2008 09:58:56 -0700 6, 20 -- X-Mailer: Apple Mail (2.753) 6, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
We tried this years ago with a group from the USGS in Woods Hole http://woodshole.er.usgs.gov/project-pages/hydrates/ using our tungsten source SEM with a cryo stage. We were not able to discern anything of interest at the time but I still think it holds promise. Some ideas - use FIB, EDS or EBSD, SIMS or even a residual gas analyzer.
Louie Kerr
mullarkay-at-gmail.com wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have someone who is interested in whether Methane Hydrate has, or } can be, studied by either SEM or TEM. He is taking a Geology class } where this came up and he came to me for information. I am totally } unfamiliar with clathrates so I turn to the wisdom on the listserve } for help. From a quick look on the web it looks like it would have to } be a cryo technique. Has or is anyone out there done/doing EM on } these? } } Thanks for any information, } } Thomas M. Murray, Ph.D. } Senior Research Support Specialist / Instructor } College of Nanoscale Science & Engineering } University at Albany-SUNY } 251 Fuller Rd. } Albany, NY 12203 } } Phone: (518) 437-8636 } Fax: (518) 437-8687 } cell: (518) 487-9535 } tmurray-at-uamail.albany.edu } } ==============================Original Headers============================== } 4, 27 -- From mullarkay-at-gmail.com Mon Mar 10 10:33:58 2008 } 4, 27 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.168]) } 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2AFXwAm007981 } 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 10 Mar 2008 10:33:58 -0500 } 4, 27 -- Received: by ug-out-1314.google.com with SMTP id c2so6330772ugf.27 } 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 10 Mar 2008 08:33:57 -0700 (PDT) } 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 27 -- d=gmail.com; s=gamma; } 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 4, 27 -- bh=5rWQFyeDMHq6LhcOgDfUso0Te0eAjr0YVYNede7P60M=; } 4, 27 -- b=XVaPJrDXB2ldmo+dm+7Lz+oj0hPUzug6wBV6F9QGlCnzwKGih14A+daYBO7MH+FlZblDPQcRgn+xmdicqZ0C26ZYaDYnguet0tkdDTJAMOawD5JeO+pDUDp0nNp/G+6hGzkkilLRehljWfK/7+yMtmsUK4iHxp9MXH5TNF1Czrg= } 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 27 -- d=gmail.com; s=gamma; } 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 4, 27 -- b=SuPEoykb0WiD8Pn7dBYQHWcV0GTfSqxH+v9ckQInETyqZTDKArnqAdZIMo4z8Hljv1ti/raXwOKNWm5+7kxIU7HZ8Ml86mwovY3DIJOxibCNJAMZkZT1t7WqhLSIbvMmFk6dTy70bmBu6dlfZKoiVE8ZlpnTOdvQh0PQQBwcZYQ= } 4, 27 -- Received: by 10.142.177.7 with SMTP id z7mr1808049wfe.47.1205163236030; } 4, 27 -- Mon, 10 Mar 2008 08:33:56 -0700 (PDT) } 4, 27 -- Received: by 10.142.191.13 with HTTP; Mon, 10 Mar 2008 08:33:55 -0700 (PDT) } 4, 27 -- Message-ID: {eeb4d15b0803100833t631051edj30991d791f6daeb4-at-mail.gmail.com} } 4, 27 -- Date: Mon, 10 Mar 2008 11:33:55 -0400 } 4, 27 -- From: "Thomas Murray" {mullarkay-at-gmail.com} } 4, 27 -- To: microscopy-at-microscopy.com } 4, 27 -- Subject: EM of Clathrates (Methane Hydrate) } 4, 27 -- MIME-Version: 1.0 } 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 4, 27 -- Content-Transfer-Encoding: 7bit } 4, 27 -- Content-Disposition: inline } ==============================End of - Headers==============================
-- Louie Kerr Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
VISIT OUR WEB SITES: http://www.mbl.edu/ http://www.courses.mbl.edu/
==============================Original Headers============================== 7, 22 -- From lkerr-at-mbl.edu Mon Mar 10 12:38:12 2008 7, 22 -- Received: from adios.mbl.edu (adios.MBL.EDU [128.128.172.10]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2AHcCJ2018907 7, 22 -- for {microscopy-at-microscopy.com} ; Mon, 10 Mar 2008 12:38:12 -0500 7, 22 -- Received: from [128.128.168.104] (unknown [128.128.168.104]) 7, 22 -- by adios.mbl.edu (Postfix) with ESMTP id 88F621CE3FB; 7, 22 -- Mon, 10 Mar 2008 13:38:11 -0400 (EDT) 7, 22 -- Message-ID: {47D57204.1000007-at-mbl.edu} 7, 22 -- Date: Mon, 10 Mar 2008 13:38:12 -0400 7, 22 -- From: Louis Kerr {lkerr-at-mbl.edu} 7, 22 -- Reply-To: lkerr-at-mbl.edu 7, 22 -- Organization: Marine Biological Laboratory 7, 22 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 7, 22 -- X-Accept-Language: en-us, en 7, 22 -- MIME-Version: 1.0 7, 22 -- To: mullarkay-at-gmail.com 7, 22 -- CC: microscopy-at-microscopy.com 7, 22 -- Subject: Re: [Microscopy] EM of Clathrates (Methane Hydrate) 7, 22 -- References: {200803101535.m2AFZkxa009580-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {200803101535.m2AFZkxa009580-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=us-ascii; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both clive.standley-at-umassmed.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Biorad MP 2100 scan head removal
Question: I have a Biorad Radiance MP 2100 using an Olympus BX50 upright scope. I want to remove the scan head, I've removed all the set screws, but the unit is still heald in place by 2 pins. How do I remove the head?
The Terminal Deoxynucleotidyl Transferase (so called Tdt) method is an elegant technique to detect DNA strands in TEM. It is very sensitive, following his author Marc Thiry. It is based on an enzymatic reaction, this is not a direct immunogold detection. However you probably won't be able to reproduce the technique just by reading the papers of the above mentioned author. Actually it seems that nobody ever could, since he is the only one to be able to use it. But I can help you offline if you wish (you may even write in french, this is my mother tongue).
Best regards,
Stephane
----- Original Message ---- X-from: "monica.nelea-at-polymtl.ca" {monica.nelea-at-polymtl.ca} To: nizets2-at-yahoo.com Sent: Saturday, March 8, 2008 4:12:26 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both monica.nelea-at-polymtl.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] immunogold staining of DNA and/or chitosan
Question: Hello, Does there is someone that know if one can perform immunogold staining for DNA and chitosan polymer or if there are other methods for staining DNA and chitosan in electron microscopy?
==============================Original Headers============================== 7, 12 -- From zaluzec-at-microscopy.com Fri Mar 7 21:02:56 2008 7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2832t6S015317 7, 12 -- for {microscopy-at-microscopy.com} ; Fri, 7 Mar 2008 21:02:55 -0600 7, 12 -- Mime-Version: 1.0 7, 12 -- Message-Id: {p06240800c3f7b252ba7a-at-[206.69.208.22]} 7, 12 -- Date: Fri, 7 Mar 2008 21:02:53 -0600 7, 12 -- To: microscopy-at-microscopy.com 7, 12 -- From: monica.nelea-at-polymtl.ca (by way of MicroscopyListserver) 7, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: immunogold staining of DNA 7, 12 -- and/or chitosan 7, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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I am working with someone in the materials section here who is embedding silica particles in a solid matrix, and would like to be able to follow their distribution. The particles are Sio2 and must be monodisperse as he sets up the reaction. The linker in the matrix is a silane derivative. It had occurred to me that it might be possible to label the particles with a fluorescent dye, and visualize them either with a confocal or widefield instrument.
Oh yes, the particles are about 0.5 micron.
Any suggestions of ways to label the particles?
Much obliged,
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 10, 19 -- From jbs-at-temple.edu Tue Mar 11 16:07:08 2008 10, 19 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2BL78GU029811 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Mar 2008 16:07:08 -0500 10, 19 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40]) 10, 19 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id m2BL793H011822 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Mar 2008 16:07:09 -0500 10, 19 -- From: "Joel Sheffield" {jbs-at-temple.edu} 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- Date: Tue, 11 Mar 2008 17:05:16 -0400 10, 19 -- MIME-Version: 1.0 10, 19 -- Subject: Labeling SiO2 particles 10, 19 -- Reply-to: jbs-at-temple.edu 10, 19 -- Message-ID: {47D6BBCC.8299.8DD4474F-at-jbs.temple.edu} 10, 19 -- Priority: normal 10, 19 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 19 -- Content-type: text/plain; charset=US-ASCII 10, 19 -- Content-transfer-encoding: 7BIT 10, 19 -- Content-description: Mail message body ==============================End of - Headers==============================
Is anyone using QImaging's RV series cameras (2000RV or 4000RV) with MetaMorph? Here's the deal - the "R" series cameras are listed as being supported by MM, the "RV" series aren't. The MM folks haven't tested the RV cameras and tell me that they aren't sure what will happen. I've had trouble contacting the western US person for QImaging to ask them what they know about compatibility issues.....so I thought I'd try the collective wisdom & experience here. I know I could just request one in for a demo, but I don't want to bother if someone has already tried this and knows it either will or won't work.
Anybody interested in such a labeling may contact me offline.
Stephane
----- Original Message ---- X-from: "jbs-at-temple.edu" {jbs-at-temple.edu} To: nizets2-at-yahoo.com Sent: Tuesday, March 11, 2008 10:18:35 PM
Hi,
I am working with someone in the materials section here who is embedding silica particles in a solid matrix, and would like to be able to follow their distribution. The particles are Sio2 and must be monodisperse as he sets up the reaction. The linker in the matrix is a silane derivative. It had occurred to me that it might be possible to label the particles with a fluorescent dye, and visualize them either with a confocal or widefield instrument.
Oh yes, the particles are about 0.5 micron.
Any suggestions of ways to label the particles?
Much obliged,
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 10, 19 -- From jbs-at-temple.edu Tue Mar 11 16:07:08 2008 10, 19 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2BL78GU029811 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Mar 2008 16:07:08 -0500 10, 19 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40]) 10, 19 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id m2BL793H011822 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Mar 2008 16:07:09 -0500 10, 19 -- From: "Joel Sheffield" {jbs-at-temple.edu} 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- Date: Tue, 11 Mar 2008 17:05:16 -0400 10, 19 -- MIME-Version: 1.0 10, 19 -- Subject: Labeling SiO2 particles 10, 19 -- Reply-to: jbs-at-temple.edu 10, 19 -- Message-ID: {47D6BBCC.8299.8DD4474F-at-jbs.temple.edu} 10, 19 -- Priority: normal 10, 19 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 19 -- Content-type: text/plain; charset=US-ASCII 10, 19 -- Content-transfer-encoding: 7BIT 10, 19 -- Content-description: Mail message body ==============================End of - Headers==============================
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==============================Original Headers============================== 21, 19 -- From nizets2-at-yahoo.com Wed Mar 12 02:26:30 2008 21, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 21, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2C7QTTC015468 21, 19 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 02:26:30 -0500 21, 19 -- Received: (qmail 91031 invoked by uid 60001); 12 Mar 2008 07:26:29 -0000 21, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 21, 19 -- s=s1024; d=yahoo.com; 21, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 21, 19 -- b=KlAEBUmDqHAaAdJ2bn/5XWM7du37lgWq9fqK9fM7x/lXbw/k3zZwOUh5ad8gqTh+ntl6noEZTEjV7W3vcDJD2H2L4lOnp5LddUJlDTMmfuinc52Je0XQ57SBtva+8gsw3vjZr/9YYfRFfX1GgN0HiCC7Qs0Chr57DmcRk4nOM2g=; 21, 19 -- X-YMail-OSG: SwRaWxEVM1kuE1woaGm3mXSH9AMwHlETep68FhIwuRFHpPKrfoFbqefe6dCC.R9heshrPuMV9lpc8C32YC5rPckLEHcrp_68plN6iaplPu5IDC3FwIJQCxKRjkpt4.0.Afr4gMK748hf93YZsIb.HszzCaBDrPQ9RScgJmsvwb_M 21, 19 -- Received: from [80.122.101.100] by web37405.mail.mud.yahoo.com via HTTP; Wed, 12 Mar 2008 00:26:28 PDT 21, 19 -- X-Mailer: YahooMailRC/902.35 YahooMailWebService/0.7.162 21, 19 -- Date: Wed, 12 Mar 2008 00:26:28 -0700 (PDT) 21, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 21, 19 -- Subject: Re: [Microscopy] Labeling SiO2 particles 21, 19 -- To: microscopy-at-microscopy.com 21, 19 -- MIME-Version: 1.0 21, 19 -- Content-Type: text/plain; charset=us-ascii 21, 19 -- Message-ID: {971261.83906.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wfkho-at-streamyx.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wfkho-at-streamyx.com Name: WF Kho
Title-Subject: [Filtered] EDS analysis on thin contaminant layer
Question: Hi,
Any one out there has experience with EDS on aluminum bond pads? Sometimes due to certain wafer fabrication process a thin flurocarbon contamination layer is formed on the pads. I notice that if I use low kV (3-5kV) and tilt the sample (60 degrees or more) I get an increase in the sensitivity of detecting the flurocarbon layer compared to the case of zero tilt. I assume by tilting the sample the interaction volume is shallower and closer to the surface. My question...is sample tilting in such circumstances acceptable and would it cause any quantification errors?
You are taking the proper steps to better detect light element films. Nothing wrong with that.
It will affect quantitation values. There are several issues. If you are looking through the film and the sub-film matrix is contributing to the spectrum, that condition must be considered for quantitation corrections. The analysis geometry (tilt) and beam potential must also be considered and likely will not match "canned" EDS quant values. Add to that, the general error uncertainty associated with quantitation of light elements.
I suspect an accurate quant would be very difficult to guarantee. Good Luck!
Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
-----Original Message----- X-from: wfkho-at-streamyx.com [mailto:wfkho-at-streamyx.com] Sent: Wednesday, March 12, 2008 9:57 AM To: White, Woody N.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both wfkho-at-streamyx.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: wfkho-at-streamyx.com Name: WF Kho
Title-Subject: [Filtered] EDS analysis on thin contaminant layer
Question: Hi,
Any one out there has experience with EDS on aluminum bond pads? Sometimes due to certain wafer fabrication process a thin flurocarbon contamination layer is formed on the pads. I notice that if I use low kV (3-5kV) and tilt the sample (60 degrees or more) I get an increase in the sensitivity of detecting the flurocarbon layer compared to the case of zero tilt. I assume by tilting the sample the interaction volume is shallower and closer to the surface. My question...is sample tilting in such circumstances acceptable and would it cause any quantification errors?
==============================Original Headers============================== 7, 11 -- From zaluzec-at-microscopy.com Wed Mar 12 08:45:41 2008 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2CDjeCi015432 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 08:45:41 -0500 7, 11 -- Mime-Version: 1.0 7, 11 -- Message-Id: {p06240801c3fd8ee39088-at-[206.69.208.22]} 7, 11 -- Date: Wed, 12 Mar 2008 08:45:39 -0500 7, 11 -- To: microscopy-at-microscopy.com 7, 11 -- From: wfkho-at-streamyx.com (by way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: EDS analysis on thin contaminant layer 7, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers============================== ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 29 -- From nwwhite-at-babcock.com Wed Mar 12 10:18:56 2008 2, 29 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2CFIuvo032500 2, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 12 Mar 2008 10:18:56 -0500 2, 29 -- Received: from ([131.184.13.224]) 2, 29 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.8169063; 2, 29 -- Wed, 12 Mar 2008 11:18:30 -0400 2, 29 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 29 -- Wed, 12 Mar 2008 11:18:30 -0400 2, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 29 -- Content-class: urn:content-classes:message 2, 29 -- MIME-Version: 1.0 2, 29 -- Subject: RE: [Microscopy] viaWWW: EDS analysis on thin contaminant layer 2, 29 -- Date: Wed, 12 Mar 2008 11:18:29 -0400 2, 29 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87B01-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 29 -- In-Reply-To: {200803121357.m2CDvBbu027876-at-ns.microscopy.com} 2, 29 -- X-MS-Has-Attach: 2, 29 -- X-MS-TNEF-Correlator: 2, 29 -- Thread-Topic: [Microscopy] viaWWW: EDS analysis on thin contaminant layer 2, 29 -- Thread-Index: AciESQePKtRUVigcQ4qDPk7c3zW/ZgACiyqw 2, 29 -- References: {200803121357.m2CDvBbu027876-at-ns.microscopy.com} 2, 29 -- To: {wfkho-at-streamyx.com} , 2, 29 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 2, 29 -- X-OriginalArrivalTime: 12 Mar 2008 15:18:30.0719 (UTC) FILETIME=[54575CF0:01C88454] 2, 29 -- From: "White, Woody N." {nwwhite-at-babcock.com} 2, 29 -- Content-Type: text/plain; 2, 29 -- charset="us-ascii" 2, 29 -- Content-Transfer-Encoding: 8bit 2, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2CFIuvo032500 ==============================End of - Headers==============================
I recommend embedding the silica in LR White - hard grade - without accelerator. Ignore all the instructions about accelerator: the accelerator is neither needed nor desired for your prep since interference of curing of the resin by oxygen is not an issue when cured at high temperatures and one does not want to use an accelerator in heat curing. Place particle/resin suspension in open embedding containers of your choice, vacuum degas, and cure overnight at 80-90C in a nitrogen-purged oven. You'll get a hard, brittle block, ideal for cutting the silica.
You are on your own about creating a monodisperse suspension of particles in the resin. If you find a good way to do that, please reply to the listserver or to me off-line.
Regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
jbs-at-temple.edu
To 03/11/08 04:13 gary.m.brown-at-exxonmobil.com PM cc
Subject Please respond [Microscopy] Labeling SiO2 particles to jbs-at-temple.edu
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi,
I am working with someone in the materials section here who is embedding silica particles in a solid matrix, and would like to be able to follow their distribution. The particles are Sio2 and must be monodisperse as he sets up the reaction. The linker in the matrix is a silane derivative. It had occurred to me that it might be possible to label the particles with a fluorescent dye, and visualize them either with a confocal or widefield instrument.
Oh yes, the particles are about 0.5 micron.
Any suggestions of ways to label the particles?
Much obliged,
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 10, 19 -- From jbs-at-temple.edu Tue Mar 11 16:07:08 2008 10, 19 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2BL78GU029811 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Mar 2008 16:07:08 -0500 10, 19 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40]) 10, 19 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id m2BL793H011822 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 11 Mar 2008 16:07:09 -0500 10, 19 -- From: "Joel Sheffield" {jbs-at-temple.edu} 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- Date: Tue, 11 Mar 2008 17:05:16 -0400 10, 19 -- MIME-Version: 1.0 10, 19 -- Subject: Labeling SiO2 particles 10, 19 -- Reply-to: jbs-at-temple.edu 10, 19 -- Message-ID: {47D6BBCC.8299.8DD4474F-at-jbs.temple.edu} 10, 19 -- Priority: normal 10, 19 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 19 -- Content-type: text/plain; charset=US-ASCII 10, 19 -- Content-transfer-encoding: 7BIT 10, 19 -- Content-description: Mail message body ==============================End of - Headers==============================
==============================Original Headers============================== 35, 19 -- From gary.m.brown-at-exxonmobil.com Wed Mar 12 10:24:15 2008 35, 19 -- Received: from dalspc01.exxonmobil.com (dalspc01.exxonmobil.com [131.126.223.1]) 35, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2CFOBj2006595 35, 19 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 10:24:13 -0500 35, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 35, 19 -- by dalspc01.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m2CFO95s009594; 35, 19 -- Wed, 12 Mar 2008 10:24:10 -0500 (CDT) 35, 19 -- In-Reply-To: {200803112113.m2BLDQr2000883-at-ns.microscopy.com} 35, 19 -- Subject: Re: [Microscopy] Labeling SiO2 particles 35, 19 -- Importance: 35, 19 -- To: jbs-at-temple.edu, microscopy-at-microscopy.com 35, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 35, 19 -- Message-ID: {OFE5311D8D.8566F938-ON8625740A.0053C3E4-8625740A.00549B27-at-exxonmobil.com} 35, 19 -- From: gary.m.brown-at-exxonmobil.com 35, 19 -- Date: Wed, 12 Mar 2008 10:24:07 -0500 35, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 35, 19 -- 02, 2006) at 03/12/2008 10:24:10 AM 35, 19 -- MIME-Version: 1.0 35, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Tilting the die will yield more volumetric interaction with the bond pad metal. How much depends on your KV. F is pretty easy to detect and discriminate since it only has a K alpha peak at 0.677eV. So the challenge is to determine what other elements you want to include in a quant. 4KV-5KV would work well for F. If you are going to include Al and Si or lighter elements, perhaps 5KV-6KV would do a nice job--these elements would also be using the K alpha peaks. If the KV is too low, you will see this as high intensity ratio error. That would mean to up the KV a little until the ratio came down. Less than 10% or so is a decent value.
When you tilt, what happens to cps and DT? Is WD the same?
gary g.
At 06:48 AM 3/12/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Quantifying this system is a exceedingly difficult problem and realistically one which should not be attempted. There are many reasons. Here are a few.
1. It is possible to analyze thin films on top of known materials but you must use a thin-film-type quantitative correction algorithm. Your EDS software might provide one.
2. It is possible to analyze tilted samples but the correction algorithm must correct for the different absorption lengths due to the oblique incidence and the foreshortened exit path. In addition, the backscatter yield will be influenced by the oblique angle. Your EDS software might provide a tilted sample correction but I'd be skeptical about the results. Quantitative correction algorithms work best the closer your sample is to a flat, polished bulk sample at normal incidence. At a moderate cost, you may get away with a single deviation from this ideal but two or more deviations - forget about it.
3. Fluorocarbons contain at a minimum F, C, O and H. They may also contain Br and Cl. Since there are no H emission lines, we must estimate H by difference. The remaining elements are low Z and thus low energy. The line intensities will all be strongly modified by absorption. Strong absorption will further complicate issues 1 and 2. At normal incidence on a bulk sample quantifying fluorocarbons is a challenge, at oblique incidence on a thin film quantification is nearly impossible.
In a system like this, the best you can realistically expect is qualitative results. You can answer questions like is there any fluorine? oxygen? carbon? bromine? You might even be able to make major/minor/trace type distinctions. Your EDS software may happily spit out quantitative results to many decimal places. Don't believe any of it. You shouldn't even trust the first decimal digit.
Nicholas Ritchie Physicist Microanalysis Group National Institute of Standards and Technology
==============================Original Headers============================== 9, 22 -- From nicholas.ritchie-at-nist.gov Wed Mar 12 12:39:48 2008 9, 22 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2CHdmGW012639 9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 12:39:48 -0500 9, 22 -- Received: from smsd-fw.nist.gov (smsd-fw.nist.gov [129.6.126.23]) 9, 22 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id m2CHdhRe012056 9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 13:39:44 -0400 9, 22 -- Received: from mag165.nist.gov by smsd-fw.nist.gov 9, 22 -- via smtpd (for webmail.nist.gov [129.6.16.228]) with ESMTP; Wed, 12 Mar 2008 13:39:44 -0400 9, 22 -- Message-ID: {47D81561.4060305-at-nist.gov} 9, 22 -- Date: Wed, 12 Mar 2008 13:39:45 -0400 9, 22 -- From: "Nicholas W. M. Ritchie" {nicholas.ritchie-at-nist.gov} 9, 22 -- Reply-To: nicholas.ritchie-at-nist.gov 9, 22 -- Organization: N.I.S.T. 9, 22 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 9, 22 -- MIME-Version: 1.0 9, 22 -- To: Microscopy-at-microscopy.com 9, 22 -- Subject: Re: viaWWW: EDS analysis on thin contaminant layer 9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-NIST-MailScanner: Found to be clean 9, 22 -- X-NIST-MailScanner-From: nicholas.ritchie-at-nist.gov ==============================End of - Headers==============================
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Email: tonygr-at-mit.edu Name: Anthony Garratt-Reed
Organization: Massachusetts Institute of Technology
Title-Subject: [Filtered] Employment opportunity
Question: The Center for Materials Science and Engineering at the Massachusetts Institute of Technology has a vacancy for a research specialist in electron microscopy. A description of the vacancy, together with MIT's employment policies, benefits, and other information, with links for on-line application is available at the following URL: http://sh.webhire.com/servlet/av/jd?ai=631&ji=2188956&sn=I or applications may be forwarded by electronic or paper mail to Ms. Susan Dalton, CMSE Assistant Director, Room # 13-2106, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307, e-mail sdalton-at-mit.edu.
The application review process will be open until a qualified candidate has been identified. Questions about this position may be addressed to the undersigned.
I recently had to remove one of the Mitutoyo positioners from our JSM-35C for lubrication (It was really sticky) and I ended up taking the sleeve off of the shaft that it attaches to. I noticed that there is an opening and a gear, which would imply that there is some kind of motorized stage option for this scope. Does anybody have this option, and if so, could you send me a photo of the mount and how it connects to the scope? It would be an interesting experiment to see if I can auto-control the stage and produce mosaic images.
--Justin A. Kraft
==============================Original Headers============================== 2, 27 -- From kraftpiano-at-gmail.com Wed Mar 12 23:49:28 2008 2, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.187]) 2, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2D4nREw032030 2, 27 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 23:49:28 -0500 2, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so2650098rvb.30 2, 27 -- for {microscopy-at-microscopy.com} ; Wed, 12 Mar 2008 21:49:27 -0700 (PDT) 2, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 27 -- d=gmail.com; s=gamma; 2, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 27 -- bh=/maNF0P23mmhUT/pwOg2yyv+Iekn4kzHOkkMEVxgs1Q=; 2, 27 -- b=sp8tyFcR0u55GZY+lovskkgpecRLbi3N+07n+Zp5Wchn3zU78A9+8K1RDKrn/VjPv065WkA4RJ1bW9djcoTqp83Qrc4QkScVwwO2kidba76EBFAPOI9uU3k6el1XJ3qFwrxz3qPooHUAfP/iHGlq8Xf6yZAYip935E5spT8rwGM= 2, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 27 -- d=gmail.com; s=gamma; 2, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 2, 27 -- b=T5HwwnskQxsUbFT6EvHqp2hHGlzZcibMXTc4r5XmET8jzZyE5qj6NG0CVI8kuOF+Y+QGPfaElEvKnmAQTB0eYwYPzSJh1HO5VppOuAho9OxviLit4iLALSVJxYiNYYJYp5aq5qjytFfaX9F9XV35oijlJktaoN95SVxLyphMzhs= 2, 27 -- Received: by 10.141.212.5 with SMTP id o5mr5601566rvq.20.1205383767263; 2, 27 -- Wed, 12 Mar 2008 21:49:27 -0700 (PDT) 2, 27 -- Received: by 10.140.161.11 with HTTP; Wed, 12 Mar 2008 21:49:27 -0700 (PDT) 2, 27 -- Message-ID: {25e2b0d20803122149y5f2aff98jeda00fce5413b7c4-at-mail.gmail.com} 2, 27 -- Date: Wed, 12 Mar 2008 23:49:27 -0500 2, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 2, 27 -- To: microscopy-at-microscopy.com 2, 27 -- Subject: JEOL JSM-35C Motorized stage? 2, 27 -- MIME-Version: 1.0 2, 27 -- Content-Type: text/plain; charset=ISO-8859-1 2, 27 -- Content-Transfer-Encoding: 7bit 2, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
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Email: f.damico-at-unict.it Name: Fabio
Organization: University of Catania, Italy
Title-Subject: [Filtered] Lowicril and Peroxidase
Question: Dear all, I have some precious low temperature Lowicryl K4M embedded specimens. I wish to know if it is possible to perform an immunocytochemical reaction on semithin sections with the use of a peroxidase streptavidin system detection. I have not found any paper on the subject.
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: CSIR
Title-Subject: [Filtered] circuit diagram for Jeol EX1200
Question: Hello,
We have Jeol EX1200 which is down because of the failure in interface. To undertake the repair work we need the circuit diagrams for the computer boards. Here is the list of faulty boards. Is anyone is having the circuit diagram for the computer boards or spare boards mentioned below for the TEM Jeol Ex1200
I don't have schematics for the JEOL733 that I am trying to get up and running. JEOL may be able to provide me with what I need, but they may have to get the schematics from Japan and that may take up to a month. I am hoping that the Probeusers list or the Microscopy list may be the key to my success.
I am specifically looking for the component connecting diagram or what may be labeled as the INTERCNDIA schematic for a 733 with the following electronics modules in the main electronics control console:
Bottom: Scan Generator Magnification Secondary Electron Image Electron Optical System Accelerating Voltage MED Camera Unit
If anyone has a configuration like this one and you have the schematic that shows how all of these are wired one to the other I would appreciate hearing from you!
Cheers Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 14, 18 -- From mmcheath-at-syr.edu Thu Mar 13 12:15:10 2008 14, 18 -- Received: from SUEXCL-02.ad.syr.edu (suexbe-02.ad.syr.edu [128.230.108.46]) 14, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2DHF99P012076 14, 18 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Mar 2008 12:15:10 -0500 14, 18 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu ([128.230.108.50]) with Microsoft Exchange Server HTTP-DAV ; 14, 18 -- Thu, 13 Mar 2008 17:15:09 +0000 14, 18 -- User-Agent: Microsoft-Entourage/11.3.3.061214 14, 18 -- Date: Thu, 13 Mar 2008 13:15:06 -0400 14, 18 -- Subject: Looking for a 733 schematic 14, 18 -- From: Michael Cheatham {mmcheath-at-syr.edu} 14, 18 -- To: {PROBEUSERS-at-LISTS.UMN.EDU} , {Microscopy-at-Microscopy.Com} 14, 18 -- Message-ID: {C3FED95A.1B7E5%mmcheath-at-syr.edu} 14, 18 -- Thread-Topic: Looking for a 733 schematic 14, 18 -- Thread-Index: AciFLchEBt2H3PEhEdyObAAWy6AryQ== 14, 18 -- Mime-version: 1.0 14, 18 -- Content-type: text/plain; 14, 18 -- charset="US-ASCII" 14, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Our EDS system is not working and I have a couple of users who need some work done.
This could be a one shot deal, or if things keep going downhill here, a longer term prospect.
I told my users I would look around for some alternatives for them. If you have any ideas, let me know.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. I can't ride this year, but I invite you to check http://www.aidslifecycle.org/108 to learn about Team Slug, a group of UCSC folks riding this year.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu Mar 13 15:10:21 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2DKAKYw001452 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 13 Mar 2008 15:10:21 -0500 8, 20 -- Received: from [128.114.125.117] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 31273554 for microscopy-at-microscopy.com; Thu, 13 Mar 2008 13:10:20 -0700 8, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 67413521; Thu, 13 Mar 2008 13:10:20 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.128] (account jmkrupp-at-ucsc.edu HELO [128.114.25.128]) 8, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 67413476 for microscopy-at-microscopy.com; Thu, 13 Mar 2008 13:10:15 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p0623090ac3ff39de771c-at-[128.114.25.128]} 8, 20 -- Date: Thu, 13 Mar 2008 13:10:14 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: EDS for hire, SF Bay area? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: wfkho-at-streamyx.com Name: WF Kho
Organization: Freescale Semiconductor
Title-Subject: [Filtered] EDS analysis on thin contaminant layer
Question: Hello All,
Thank you very much for the advice from everyone.
I supppose only qualitative results can be obtained in analysing such a sample.
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Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Cooperative Oxford Laboratory
Title-Subject: [Filtered] chemical substitution
Question: I have been asked to research options to substitute Spurr's epoxy resin kit, propylene oxide and osmium tetroxide for non hazardous chemicals.
Has anyone tried and liked Quetol-651 in place of Spurr's? Do you know of other options that work well for low viscosity epoxy resins?
Do you like/prefer acetone in place of chloroform?
Do you like acetonitrile in place of propylene oxide?
I am relativley new to EM and prefer to use the old standards and am afraid to try new. Your expert opinions would be greatly appreciated.
You need osmium to "stain" lipids (make them electron dense) and give contrast to the tissue in the EM. Unosmicated tissue is very difficult to see in the EM. I have not used Quetol but it is worth a try. The new formulation of Spurr (replacement of ERL 4206 with ERL 4221) has resulted in increased viscosity. However, all of the epoxy resins are toxic so I don't know what you might gain with Quetol. Most embedding media (Epon, Spurr, Araldite) will mix with absolute ethanol, just not as quickly as prop. oxide does. So you can omit the prop.oxide if you are careful to remove all of the alcohol. I don't know about Quetol but a good EM techniques book could tell you.
Geoff
sue.tyler-at-noaa.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: sue.tyler-at-noaa.gov } Name: Sue Tyler } } Organization: Cooperative Oxford Laboratory } } Title-Subject: [Filtered] chemical substitution } } Question: I have been asked to research options to substitute Spurr's } epoxy resin kit, propylene oxide and osmium tetroxide for non } hazardous chemicals. } } Has anyone tried and liked Quetol-651 in place of Spurr's? Do you } know of other options that work well for low viscosity epoxy resins? } } Do you like/prefer acetone in place of chloroform? } } Do you like acetonitrile in place of propylene oxide? } } I am relativley new to EM and prefer to use the old standards and am } afraid to try new. Your expert opinions would be greatly appreciated. } } Thanks, } Sue } } Login Host: 205.156.36.37 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Fri Mar 14 09:24:44 2008 } 11, 11 -- Received: from [192.168.50.33] (msdvpn8.msd.anl.gov [130.202.238.72]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EEOggg027011 } 11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 09:24:44 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240802c4003b12f731-at-[192.168.50.33]} } 11, 11 -- Date: Fri, 14 Mar 2008 09:24:43 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: chemical substitution } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 28 -- From mcauliff-at-umdnj.edu Fri Mar 14 13:09:27 2008 8, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EI9PlD031845 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 13:09:25 -0500 8, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 8, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 82E31A7BFA 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 14:09:23 -0400 (EDT) 8, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 8, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 7BE13A7BE8 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 14:09:22 -0400 (EDT) 8, 28 -- Received: from ([10.32.15.171]) 8, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.144869851; 8, 28 -- Fri, 14 Mar 2008 14:09:03 -0400 8, 28 -- MIME-version: 1.0 8, 28 -- Content-transfer-encoding: 7BIT 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 8, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 8, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0JXQ00BR6FR22B00-at-umduwc02.umdnj.edu} for 8, 28 -- microscopy-at-microscopy.com; Fri, 14 Mar 2008 14:09:03 -0400 (EDT) 8, 28 -- Message-id: {47DABF5D.4000903-at-umdnj.edu} 8, 28 -- Date: Fri, 14 Mar 2008 14:09:33 -0400 8, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 28 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 8, 28 -- To: sue.tyler-at-noaa.gov, microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] viaWWW: chemical substitution 8, 28 -- References: {200803141426.m2EEQ8tJ029967-at-ns.microscopy.com} 8, 28 -- In-reply-to: {200803141426.m2EEQ8tJ029967-at-ns.microscopy.com} ==============================End of - Headers==============================
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Organization: Fred Hutchinson Cancer Research Center in Seattle, WA
Title-Subject: [Filtered] Employment Opportunity
Question: The Fred Hutchinson Cancer Research Electron Microscopy Resource has a vacancy for an Electron Microscopy Specialist. A description of the vacancy, employment policies, benefits, other information, and an on-line application is available at the following URL: http://www.fhcrc.org/about/jobs/
The application review process will be open until a qualified applicant has been identified. Questions about this position may be e-mailed to the above address and person.
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Email: ulutas-at-ieee.org Name: Mustafa Ulutas
Organization: Ondokuz Mayis University
Title-Subject: [Filtered] Noran Instruments, Voyager II, Software
Question: My question is on the Voyager II X-Ray Microanalysis Software running on a SparcStation IPX. The system was up and running until a hardware failure of the SCSI hard disk. Now we can not boot the system at all.
The Company's (Noran Instruments) web site (www.noran.com) seems to be dead too.
I'm planning to run QEMU on a x86 PC with a PCI SCSI adapter for VME controller board to install the OS (SunOS 4.1.1) and analysis software (Voyager II) from SCSI backup tapes.
As many of you have learned the BioMeda Company the manufacturer of the popular Crystal Mount, Gel Mount and similar products have not been available in the marketplace for quite a few months This has caused an uproar in the community and has left many customers stranded. We have been trying to address this issue and finally we have succeeded. We have been able to locate a former employee of the BioMeda company and they been manufacturing these formulations for themselves We have had the opportunity to test all of the new manufacturers products and we are elated to report that we can find any difference in the new product vs the original formulation
With this in mind we do have available from the new supplier the Gel and Crystal mount in all of the original sizes. Our part number is as follows:
17985-10 Gel-Mount 20 ml 17985-12 Crystal-Mount 30ml 17985-15 Crystal Mount 250 ml
If you have any questions please feel free to contact us
Thank you and we look forward to hearing from you
Stacie Kirsch Electron Microscopy Sciences 1560 Industry Road Hatfield Pa 19440 Tel: 215-412-8400 Fax: 215-412-8450 www.emsdiasum.com
==============================Original Headers============================== 9, 23 -- From SGKCCK-at-aol.com Fri Mar 14 13:51:15 2008 9, 23 -- Received: from imo-m25.mx.aol.com (imo-m25.mx.aol.com [64.12.137.6]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EIpEsp006582 9, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 13:51:15 -0500 9, 23 -- Received: from SGKCCK-at-aol.com 9, 23 -- by imo-m25.mx.aol.com (mail_out_v38_r9.3.) id w.c3c.2e2a810b (37117); 9, 23 -- Fri, 14 Mar 2008 14:51:09 -0400 (EDT) 9, 23 -- Received: from WEBMAIL-MB06 (webmail-mb06.webmail.aol.com [64.12.170.154]) by cia-ma01.mx.aol.com (v121.4) with ESMTP id MAILCIAMA013-90fd47dac91ced; Fri, 14 Mar 2008 14:51:08 -0400 9, 23 -- To: Microscopy-at-microscopy.com 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- Subject: BioMeda Gel Mount, Crystal Mount ETC 9, 23 -- Date: Fri, 14 Mar 2008 14:51:08 -0400 9, 23 -- X-AOL-IP: 141.158.58.97 9, 23 -- X-MB-Message-Source: WebUI 9, 23 -- Received: from 141.158.58.97 by WEBMAIL-MB06.sysops.aol.com (64.12.170.154) with HTTP (WebMailUI); Fri, 14 Mar 2008 14:51:08 -0400 9, 23 -- MIME-Version: 1.0 9, 23 -- From: sgkcck-at-aol.com 9, 23 -- X-MB-Message-Type: User 9, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 9, 23 -- X-Mailer: AOL Webmail 35304-STANDARD 9, 23 -- Cc: Histonet-requests-at-lists.utsouthwestern.edu, CONFOCAL-at-LISTSERV.BUFFALO.EDU 9, 23 -- Message-Id: {8CA541B7FA075D0-16E8-C5B-at-WEBMAIL-MB06.sysops.aol.com} 9, 23 -- X-Spam-Flag: NO ==============================End of - Headers==============================
Spurr's is no more since the most hazardous component was eliminated and replaced by ERL-4221, a more viscous ingredient that needs a different proportion in the mix. This was recently discussed on the list (check the archives), but in a nutshell make sure you have current info on the new component ratios. See the Ellis paper in Microscopy Today: E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006
I have used her Low Viscosity formula with Quetol-651 and it is a very nice resin in my opinion. Trims and sections VERY nicely and the sections seem very stable in the beam. Stains nicely too. I did not have BDMA so I used DMAE at 0.09g/10g "batch" (according to the Ellis formulas) and it is well polymerized at 16hr -at- 70C (I like a slow set mix). The 0.09g is just 5 drops from a Samco #241 plastic pipet; 4 drops took 2 day to harden, but was good then.
You can use acetone straight through, or ethanol, then change to acetone for infiltration. I don't need to use propylene oxide. There is much excellent work published that didn't use it.
Osmium tetroxide is dangerous - the vapor - but put the waste in a bottle with some vegetable oil and it is reduced and less of a problem to have around until waste pick-up. If there are chemists out there, please tell us the facts, but the reduced osmium product is not much of a concern (but still handle cautiously).
Wikipedia: Osmium in a metallic form is extremely dense, blue-white, brittle, and lustrous even at high temperatures, but proves to be extremely difficult to make. Powdered osmium is easier to make, but powdered osmium exposed to air leads to the formation of osmium tetroxide (OsO4), which is very toxic. The tetroxide is a powerful oxidizing agent, very volatile, water-soluble, pale yellow, crystalline solid with a strong smell that boils at 130°C. By contrast osmium dioxide (OsO2) is black, non-volatile and much less reactive and toxic.
Spurr-replacement "New Formulation" E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006
Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations"
All formulas are for a "10g batch" - with reference to the (epoxy + acid anhydride)
4.10 5.90 0.95 0.10 Hard 4.10 5.90 1.43 0.10 Standard 4.10 5.90 1.90 0.10 Soft
Low Viscosity Formula (Half the viscosity of the "Refomulated Spurr's") ------------------------- ERL 4221 2.22g Quetol-651 1.40g NSA 6.38g DER-736 1.43g BDMA 0.2g Some think BDMA should be less, 0.1g I (DAC) used 0.09g DMAE (5 drops from a Samco #241 pipet) and it set in 16h -at- 70C
Dale Callaham
sue.tyler-at-noaa.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: sue.tyler-at-noaa.gov } Name: Sue Tyler } } Organization: Cooperative Oxford Laboratory } } Title-Subject: [Filtered] chemical substitution } } Question: I have been asked to research options to substitute Spurr's } epoxy resin kit, propylene oxide and osmium tetroxide for non } hazardous chemicals. } } Has anyone tried and liked Quetol-651 in place of Spurr's? Do you } know of other options that work well for low viscosity epoxy resins? } } Do you like/prefer acetone in place of chloroform? } } Do you like acetonitrile in place of propylene oxide? } } I am relativley new to EM and prefer to use the old standards and am } afraid to try new. Your expert opinions would be greatly appreciated. } } Thanks, } Sue } } Login Host: 205.156.36.37 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Fri Mar 14 09:24:44 2008 } 11, 11 -- Received: from [192.168.50.33] (msdvpn8.msd.anl.gov [130.202.238.72]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EEOggg027011 } 11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 09:24:44 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240802c4003b12f731-at-[192.168.50.33]} } 11, 11 -- Date: Fri, 14 Mar 2008 09:24:43 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: chemical substitution } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 22 -- From dac-at-research.umass.edu Fri Mar 14 15:07:14 2008 17, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EK7DKR022779 17, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 15:07:14 -0500 17, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 17, 22 -- (authenticated bits=0) 17, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m2EK7DST009293 17, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 17, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 16:07:13 -0400 17, 22 -- Message-ID: {47DAE9F2.1010207-at-research.umass.edu} 17, 22 -- Date: Fri, 14 Mar 2008 16:11:14 -0500 17, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 17, 22 -- Reply-To: dac-at-research.umass.edu 17, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 17, 22 -- MIME-Version: 1.0 17, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 17, 22 -- Subject: Re: [Microscopy] viaWWW: chemical substitution 17, 22 -- References: {200803141435.m2EEZkqc012209-at-ns.microscopy.com} 17, 22 -- In-Reply-To: {200803141435.m2EEZkqc012209-at-ns.microscopy.com} 17, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 22 -- Content-Transfer-Encoding: 8bit 17, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Dear friends is there any feedback available from people using Deep See for applications with small animals? All the best Alby ---------------------------------------------------- "Water slowly flowed under the sky" (Cesare Pavese) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 6, 21 -- From sekkio-at-mac.com Sat Mar 15 02:59:51 2008 6, 21 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com [17.148.16.83]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2F7xod3026772 6, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Mar 2008 02:59:50 -0500 6, 21 -- Received: from mac.com (asmtp004-s [10.150.69.67]) 6, 21 -- by smtpoutm.mac.com (Xserve/smtpout020/MantshX 4.0) with ESMTP id m2F7xo7A010812 6, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Mar 2008 00:59:50 -0700 (PDT) 6, 21 -- Received: from [10.0.1.2] (host121-123-dynamic.8-87-r.retail.telecomitalia.it [87.8.123.121]) 6, 21 -- (authenticated bits=0) 6, 21 -- by mac.com (Xserve/asmtp004/MantshX 4.0) with ESMTP id m2F7xkKm003888 6, 21 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 6, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 15 Mar 2008 00:59:49 -0700 (PDT) 6, 21 -- Message-Id: {B0AA5D8D-D087-4A7F-A35B-0F0E56C27CF7-at-mac.com} 6, 21 -- From: Diaspro {sekkio-at-mac.com} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v919.2) 6, 21 -- Subject: is there any feedback using Deep See ? 6, 21 -- Date: Sat, 15 Mar 2008 08:59:46 +0100 6, 21 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
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Question: Hello To The Group Members, I am looking at teh autifluorescence of rice roots. I have following questions: The books say the following: 1. Suberin gives blue colour 2. Lipids/phenolic compounds give blue colour 3. Lignin gives blue/yellow green colour How do I interpret the results? I have tried specific stains,but still I believe there is green autofluorescence, and what they would say? Regards, RACHEL.
Our SEM pump stopped working today. Its spring break and there is nobody around till Monday. Please advise how should I leave the machine until someone can fix it.
thanks.
Sau
Send instant messages to your online friends http://uk.messenger.yahoo.com
==============================Original Headers============================== 6, 21 -- From sau_silwal-at-yahoo.com Sat Mar 15 14:14:56 2008 6, 21 -- Received: from web30108.mail.mud.yahoo.com (web30108.mail.mud.yahoo.com [209.191.69.40]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2FJEuwB011985 6, 21 -- for {Microscopy-at-Microscopy.Com} ; Sat, 15 Mar 2008 14:14:56 -0500 6, 21 -- Received: (qmail 7835 invoked by uid 60001); 15 Mar 2008 19:14:55 -0000 6, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 21 -- s=s1024; d=yahoo.com; 6, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 21 -- b=ZLDj6+DDZiKBuDC0pOzqKfbBdvQcXti5V1Y8dxdcJIrnBLjeIUl47eD8iVc0HDrHC00JqSU2wwzZm94zni0S50mMxOqykwyjpHxi+a0L5iSwr0kwThLv3rCzjwXRFdtJ1QiNWTHYGyr7Blc3oqkp9rV0QHRqYN1FE63BFPkir9I=; 6, 21 -- X-YMail-OSG: 9MDzwJgVM1kXqW_tfoo25i..rjudzWDK.bQpnBtlx9UHn2erkgaPa0yGho4pH3l1uyWzhaHSp5gFZCyNlKIzYtTyaw4JohpKMKpy5S6uVvLXMor3HTDVTnvH5bwj2A-- 6, 21 -- Received: from [152.13.163.122] by web30108.mail.mud.yahoo.com via HTTP; Sat, 15 Mar 2008 12:14:55 PDT 6, 21 -- X-Mailer: YahooMailRC/902.38 YahooMailWebService/0.7.185 6, 21 -- Date: Sat, 15 Mar 2008 12:14:55 -0700 (PDT) 6, 21 -- From: Saubhagya Silwal {sau_silwal-at-yahoo.com} 6, 21 -- Subject: SEM PUMP 6, 21 -- To: Microscopy-at-Microscopy.Com 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; charset=utf-8 6, 21 -- Message-ID: {618846.7435.qm-at-web30108.mail.mud.yahoo.com} 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2FJEuwB011985 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (Kathrine.Scholtz-at-students.wits.ac.za) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, March 15, 2008 at 12:15:07 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Kathrine.Scholtz-at-students.wits.ac.za as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: Kathrine.Scholtz-at-students.wits.ac.za Name: Kate Scholtz
Organization: University of the Witwatersrand
Education: Graduate College
Location: Johannesburg, South Africa
Title: Scanning electron microscopy
Question: Hi I am a graduate student from South Africa currently visiting a laboratory at Harvard University. I am currently attempting to view Vascular Smooth Muscle cells and cardiac myocytes using a Scanning Electron Microscope. I am fixing the cells with 3% Gluteraldehyde/3% Paraformaldehyde in 0.2M Sodium Cacodylate buffer, 1% Osmium Tetroxide and dehydrating in a series of graded ethanol into 100%. Following dehydration I am processing the cells in HMDS in varying concentrations (1:3; 1:1 and 3:1) in 100% ethanol and then leaving it in an incubator at 37C overnight to dehydrate. I am using HMDS as CPD resulted in large surface cracks and generally very ppor preservation. While the HMDS appears to work better I am still not getting good results. The cell membranes of all the cells are being pulled off exposing the inner cell structures. Does anyone know of a good way to keep the cell membranes intact? Or maybe suggestions on what I might be doing wrong to cause the ripping off of the cell membrane?
Kate, The one thing that pops out to me is that the etoh/hmds series should end with 100% hmds, 2 or 3 times, and then air dry. After removing the water, all the alcohol needs to be removed. Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Kathrine.Scholtz-at-students.wits.ac.za [mailto:Kathrine.Scholtz-at-students.wits.ac.za] Sent: Saturday, March 15, 2008 8:02 PM To: kenconverse-at-qualityimages.biz
This Question was submitted to Ask-A-Microscopist by (Kathrine.Scholtz-at-students.wits.ac.za) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, March 15, 2008 at 12:15:07 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Kathrine.Scholtz-at-students.wits.ac.za as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: Kathrine.Scholtz-at-students.wits.ac.za Name: Kate Scholtz
Organization: University of the Witwatersrand
Education: Graduate College
Location: Johannesburg, South Africa
Title: Scanning electron microscopy
Question: Hi I am a graduate student from South Africa currently visiting a laboratory at Harvard University. I am currently attempting to view Vascular Smooth Muscle cells and cardiac myocytes using a Scanning Electron Microscope. I am fixing the cells with 3% Gluteraldehyde/3% Paraformaldehyde in 0.2M Sodium Cacodylate buffer, 1% Osmium Tetroxide and dehydrating in a series of graded ethanol into 100%. Following dehydration I am processing the cells in HMDS in varying concentrations (1:3; 1:1 and 3:1) in 100% ethanol and then leaving it in an incubator at 37C overnight to dehydrate. I am using HMDS as CPD resulted in large surface cracks and generally very ppor preservation. While the HMDS appears to work better I am still not getting good results. The cell membranes of all the cells are being pulled off exposing the inner cell structures. Does anyone know of a good way to keep the cell membranes intact? Or maybe suggestions on what I might be doing wrong to cause the ripping off of the cell membrane?
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Email: barkdoll-at-gmail.com Name: Edwin Barkdoll
Title-Subject: [Filtered] battery for Zeiss M35 body
Question: Can anyone tell me what battery the Ziess M35 body takes? Thanks, Edwin
These older cameras used the 1.35V Hg battery. The new replacement is the Wein Zinc-Air PX625 1.35V cell.
You should be able to find this unit and I "think" that it will work.
gary g.
At 02:04 PM 3/16/2008, you wrote:
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==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Sun Mar 16 18:52:22 2008 7, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2GNqMlN009237 7, 20 -- for {microscopy-at-microscopy.com} ; Sun, 16 Mar 2008 18:52:22 -0500 7, 20 -- Message-Id: {200803162352.m2GNqMlN009237-at-ns.microscopy.com} 7, 20 -- Received: (qmail 20903 invoked from network); 16 Mar 2008 16:51:21 -0700 7, 20 -- Received: by simscan 1.1.0 ppid: 20900, pid: 20901, t: 0.0776s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by qsmtp4 with SMTP; 16 Mar 2008 16:51:20 -0700 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Sun, 16 Mar 2008 16:52:17 -0700 7, 20 -- To: barkdoll-at-gmail.com 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: battery for Zeiss M35 body 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200803162104.m2GL4BDF026166-at-ns.microscopy.com} 7, 20 -- References: {200803162104.m2GL4BDF026166-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-26AF225B ==============================End of - Headers==============================
As already discussed in the list, replacing Propyleneoxide with acetonitrile or acetone is clearly possible. As for chloroform, I can only guess you use it to flatten your ultrathin sections. The "elegant" way is to use a heating pen. I know nothing which could replace OsO4, but neutralizing it is possible (use unsaturated oil).
Regards, Stephane
----- Original Message ---- X-from: "sue.tyler-at-noaa.gov" {sue.tyler-at-noaa.gov} To: nizets2-at-yahoo.com Sent: Friday, March 14, 2008 3:33:08 PM
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Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Cooperative Oxford Laboratory
Title-Subject: [Filtered] chemical substitution
Question: I have been asked to research options to substitute Spurr's epoxy resin kit, propylene oxide and osmium tetroxide for non hazardous chemicals.
Has anyone tried and liked Quetol-651 in place of Spurr's? Do you know of other options that work well for low viscosity epoxy resins?
Do you like/prefer acetone in place of chloroform?
Do you like acetonitrile in place of propylene oxide?
I am relativley new to EM and prefer to use the old standards and am afraid to try new. Your expert opinions would be greatly appreciated.
==============================Original Headers============================== 11, 11 -- From zaluzec-at-microscopy.com Fri Mar 14 09:24:44 2008 11, 11 -- Received: from [192.168.50.33] (msdvpn8.msd.anl.gov [130.202.238.72]) 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EEOggg027011 11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 09:24:44 -0500 11, 11 -- Mime-Version: 1.0 11, 11 -- Message-Id: {p06240802c4003b12f731-at-[192.168.50.33]} 11, 11 -- Date: Fri, 14 Mar 2008 09:24:43 -0500 11, 11 -- To: microscopy-at-microscopy.com 11, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver) 11, 11 -- Subject: viaWWW: chemical substitution 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 23, 20 -- From nizets2-at-yahoo.com Mon Mar 17 06:58:55 2008 23, 20 -- Received: from web37409.mail.mud.yahoo.com (web37409.mail.mud.yahoo.com [209.191.91.141]) 23, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2HBwtWB032656 23, 20 -- for {microscopy-at-microscopy.com} ; Mon, 17 Mar 2008 06:58:55 -0500 23, 20 -- Received: (qmail 96491 invoked by uid 60001); 17 Mar 2008 11:58:54 -0000 23, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 20 -- s=s1024; d=yahoo.com; 23, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 23, 20 -- b=M7RC5VAZL1/che600ghNIFZe7NGn4wRbN5lHtxmWSHDlQXvG2C7SixbV468+fKiN1qjExTyaUTsMfXGA83udq86oI2Ni0N4Bc4gBqvK8cdKTFWpELOD2pIcOi6Wdn4LcUPLMw8Ch0ifYjmuUmOfxcKD/0vFfI86NrnBY5sDkKGY=; 23, 20 -- X-YMail-OSG: TzWtT1sVM1naBfRfcl31zgVz93AWN1rVxS3OirrwWI8x.kFJXUGfP3Eh.d38u7riflnzxq1DhE.gVFoJGW5wVJ11YpQD.IVzXXn3Fs_lqJUxryFFaRpyneo_e7ZD3q.k28GlnLQiPGPt8xSJbBccRn03OjU6.CEJWWu2pNwG6HxvkaDYNc03JJ8- 23, 20 -- Received: from [80.122.101.100] by web37409.mail.mud.yahoo.com via HTTP; Mon, 17 Mar 2008 04:58:54 PDT 23, 20 -- X-Mailer: YahooMailRC/902.38 YahooMailWebService/0.7.162 23, 20 -- Date: Mon, 17 Mar 2008 04:58:54 -0700 (PDT) 23, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 23, 20 -- Subject: Re: [Microscopy] viaWWW: chemical substitution 23, 20 -- To: sue.tyler-at-noaa.gov 23, 20 -- Cc: microscopy-at-microscopy.com 23, 20 -- MIME-Version: 1.0 23, 20 -- Content-Type: text/plain; charset=us-ascii 23, 20 -- Message-ID: {809459.95536.qm-at-web37409.mail.mud.yahoo.com} ==============================End of - Headers==============================
As previously mentioned, be sure to dry from pure HMDS. This usually takes 3 exchanges in 100% HMDS after the final 1:3 EtOH:HMDS. You might also try raising the drying temperature to 45 or 60 deg C.
Another thing that will help the membrane preservation is first, reduce the concentration of glutaraldehyde. 3% seems rather high, try 1.25%. Next, add 1% monomeric tannic acid to the glut and/or the osmium. Tannic acid works well to help preserve membranes. Sometimes it works best in one or the other of the primary or secondary fixatives, sometimes it works best in both.
Phil
} Email: Kathrine.Scholtz-at-students.wits.ac.za } Name: Kate Scholtz } } Organization: University of the Witwatersrand } } Education: Graduate College } } Location: Johannesburg, South Africa } } Title: Scanning electron microscopy } } Question: Hi } I am a graduate student from South Africa currently visiting a } laboratory at Harvard University. I am currently attempting to view } Vascular Smooth Muscle cells and cardiac myocytes using a Scanning } Electron Microscope. I am fixing the cells with 3% Gluteraldehyde/3% } Paraformaldehyde in 0.2M Sodium Cacodylate buffer, 1% Osmium } Tetroxide and dehydrating in a series of graded ethanol into 100%. } Following dehydration I am processing the cells in HMDS in varying } concentrations (1:3; 1:1 and 3:1) in 100% ethanol and then leaving it } in an incubator at 37C overnight to dehydrate. I am using HMDS as CPD } resulted in large surface cracks and generally very ppor } preservation. While the HMDS appears to work better I am still not } getting good results. The cell membranes of all the cells are being } pulled off exposing the inner cell structures. Does anyone know of a } good way to keep the cell membranes intact? Or maybe suggestions on } what I might be doing wrong to cause the ripping off of the cell } membrane? } } Thank you in advance } } Kind Regards } Kate Scholtz -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 24 -- From oshel1pe-at-cmich.edu Mon Mar 17 07:36:44 2008 5, 24 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2HCaiAs015464 5, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Mar 2008 07:36:44 -0500 5, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 24 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m2HCpF9N016338; 5, 24 -- Mon, 17 Mar 2008 08:51:16 -0400 5, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 5, 24 -- Mon, 17 Mar 2008 08:36:38 -0400 5, 24 -- Mime-Version: 1.0 5, 24 -- Message-Id: {f06240802c404153619e0-at-[141.209.160.249]} 5, 24 -- In-Reply-To: {200803160003.m2G03WHU012709-at-ns.microscopy.com} 5, 24 -- References: {200803160003.m2G03WHU012709-at-ns.microscopy.com} 5, 24 -- Date: Mon, 17 Mar 2008 08:36:37 -0400 5, 24 -- To: Kathrine.Scholtz-at-students.wits.ac.za 5, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 24 -- Subject: Re: [Microscopy] AskAMicroscopist :Vascular Smooth Muscle cells 5, 24 -- and cardiac 5, 24 -- Cc: Microscopy-at-microscopy.com 5, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 24 -- X-OriginalArrivalTime: 17 Mar 2008 12:36:39.0182 (UTC) FILETIME=[8BE12AE0:01C8882B] 5, 24 -- X-CanItPRO-Stream: default 5, 24 -- X-Spam-Score: -3.4 () J_CHICKENPOX_44,L_EXCH_MF 5, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
One thing to remember is that after your HMDS treatment, you need to be careful to keep your specimen away from humidity. Specifically, if that incubator has Petri dishes or other moist things in there, your specimen may be partially re-hydrating as it sits in there overnight. In my experience, HMDS dries very quickly with no need for higher temperatures. I would suggest that you allow the sample to air-dry, at ambient temperature, for 5-10 minutes in a fume hood and then transfer it to a dessicant cabinet or a bell jar with nice, blue dri-rite or silica gel. You can sort of wave the specimen at your nose periodically to see if the smell of HMDS is gone, and then move it to the dessicant (or straight into the coater). I think you are on the right track with high glut and OsO4; however, ethanol (especially absolute) can be highly extractive of lipids, and you say you are having trouble with the membranes, so I would also suggest you also try to minimize your exposure to ethanol. If these are monolayers of cells on glass, you can go as low as 5-10 minutes on those ethanol and HMDS exchanges.
Feel free to email me if you need more details of any of the above.
-----Original Message----- X-from: Kathrine.Scholtz-at-students.wits.ac.za [mailto:Kathrine.Scholtz-at-students.wits.ac.za] Sent: Saturday, March 15, 2008 6:07 PM To: Bowling, Andrew
This Question was submitted to Ask-A-Microscopist by (Kathrine.Scholtz-at-students.wits.ac.za) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, March 15, 2008 at 12:15:07 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both Kathrine.Scholtz-at-students.wits.ac.za as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: Kathrine.Scholtz-at-students.wits.ac.za Name: Kate Scholtz
Organization: University of the Witwatersrand
Education: Graduate College
Location: Johannesburg, South Africa
Title: Scanning electron microscopy
Question: Hi I am a graduate student from South Africa currently visiting a laboratory at Harvard University. I am currently attempting to view Vascular Smooth Muscle cells and cardiac myocytes using a Scanning Electron Microscope. I am fixing the cells with 3% Gluteraldehyde/3% Paraformaldehyde in 0.2M Sodium Cacodylate buffer, 1% Osmium Tetroxide and dehydrating in a series of graded ethanol into 100%. Following dehydration I am processing the cells in HMDS in varying concentrations (1:3; 1:1 and 3:1) in 100% ethanol and then leaving it in an incubator at 37C overnight to dehydrate. I am using HMDS as CPD resulted in large surface cracks and generally very ppor preservation. While the HMDS appears to work better I am still not getting good results. The cell membranes of all the cells are being pulled off exposing the inner cell structures. Does anyone know of a good way to keep the cell membranes intact? Or maybe suggestions on what I might be doing wrong to cause the ripping off of the cell membrane?
==============================Original Headers============================== 10, 12 -- From zaluzec-at-microscopy.com Sat Mar 15 18:58:11 2008 10, 12 -- Received: from [192.168.50.30] (msdvpn8.msd.anl.gov [130.202.238.72]) 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2FNw9aS006300 10, 12 -- for {microscopy-at-microscopy.com} ; Sat, 15 Mar 2008 18:58:10 -0500 10, 12 -- Mime-Version: 1.0 10, 12 -- Message-Id: {p06240800c40212e8cfab-at-[192.168.50.33]} 10, 12 -- Date: Sat, 15 Mar 2008 18:58:10 -0500 10, 12 -- To: microscopy-at-microscopy.com 10, 12 -- From: Kathrine.Scholtz-at-students.wits.ac.za (by way of Ask-A-Microscopist) 10, 12 -- Subject: AskAMicroscopist :Vascular Smooth Muscle cells and cardiac 10, 12 -- myocytes using a Scanning Electron Microscope 10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 19, 31 -- From Andrew.Bowling-at-ARS.USDA.GOV Mon Mar 17 13:50:15 2008 19, 31 -- Received: from messagescreen3.ars.usda.gov (messagescreen3.ars.usda.gov [199.133.180.150]) 19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2HIoEiX020037 19, 31 -- for {microscopy-at-microscopy.com} ; Mon, 17 Mar 2008 13:50:15 -0500 19, 31 -- Received: from CO-MAILBH-02.ARSNET.ARS.USDA.GOV ([199.133.183.227]) 19, 31 -- by messagescreen3.ars.usda.gov (8.13.8/8.13.8) with ESMTP id m2HIlYtX031949; 19, 31 -- Mon, 17 Mar 2008 13:50:13 -0500 19, 31 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 19, 31 -- Mon, 17 Mar 2008 12:49:41 -0600 19, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 31 -- Content-class: urn:content-classes:message 19, 31 -- MIME-Version: 1.0 19, 31 -- Content-Type: text/plain; 19, 31 -- charset="us-ascii" 19, 31 -- Subject: RE: [Microscopy] AskAMicroscopist :Vascular Smooth Muscle cells and cardiac 19, 31 -- Date: Mon, 17 Mar 2008 12:49:41 -0600 19, 31 -- Message-ID: {8017F94146BF634DA9414E4B9088525BEE7B8C-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 19, 31 -- In-Reply-To: {200803160007.m2G07OpX016563-at-ns.microscopy.com} 19, 31 -- X-MS-Has-Attach: 19, 31 -- X-MS-TNEF-Correlator: 19, 31 -- Thread-Topic: [Microscopy] AskAMicroscopist :Vascular Smooth Muscle cells and cardiac 19, 31 -- Thread-Index: AciG+bfUPYjDKr7HSaWtRR9XAdbdGgBavY+A 19, 31 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 19, 31 -- To: {Kathrine.Scholtz-at-students.wits.ac.za} , {microscopy-at-microscopy.com} 19, 31 -- X-OriginalArrivalTime: 17 Mar 2008 18:49:41.0963 (UTC) FILETIME=[A90E81B0:01C8885F] 19, 31 -- X-MessageScreenMessageID: 1205779814.974506.1253.187711515 19, 31 -- X-MessageScreenContentScore: Score of 0 assigned to Content 19, 31 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 19, 31 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 19, 31 -- Content-Transfer-Encoding: 8bit 19, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2HIoEiX020037 ==============================End of - Headers==============================
From zaluzec-at-aaem.amc.anl.gov Thu Mar 20 08:16:48 2008 Return-Path: {zaluzec-at-aaem.amc.anl.gov} Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2KDBUAk011767; Thu, 20 Mar 2008 08:16:47 -0500 Received: from [146.139.72.120] (aem120.amc.anl.gov [146.139.72.120]) by aaem.amc.anl.gov (8.12.11.20060308/8.12.10) with ESMTP id m2JEUPru020932; Wed, 19 Mar 2008 09:30:25 -0500 Mime-Version: 1.0 (Apple Message framework v753) Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Message-Id: {A84AE369-EA7E-495A-A4F2-5507A911B32E-at-aaem.amc.anl.gov} Cc: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} Content-Transfer-Encoding: 7bit
Colleagues...
DId you enjoy the quiet time? I didn't, resorting to old phone line connections was painfully slow.
In any event, the hard line failure is now fixed. You may resume postings.
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 5, 11 -- From zaluzec-at-microscopy.com Thu Mar 20 08:18:16 2008 5, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2KDI4nC014283 5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 08:18:16 -0500 5, 11 -- Mime-Version: 1.0 5, 11 -- Message-Id: {p06240800c40813f9b32a-at-[206.69.208.22]} 5, 11 -- Date: Thu, 20 Mar 2008 08:18:06 -0500 5, 11 -- To: microscopy-at-microscopy.com 5, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 5, 11 -- Subject: Administrivia: Network Back 0n-Line 5, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I'd like to point out from the previous post that corn oil is more effective than vegetable oil for somewhat neutralizing osmium tetroxide due to its higher percentage of unsaturated bonds, and more than "some" should probably be used. Below is a link that describes a specific proportion of corn oil to better ensure safety, which was obtained through a basic web search.
I just wanted to make sure no one is working with osmium while only half informed. This stuff is not a substance to use without full understanding.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Friday, March 14, 2008 4:17 PM To: Sobocinski, Gregg
Hi Sue,
Spurr's is no more since the most hazardous component was eliminated and replaced by ERL-4221, a more viscous ingredient that needs a different proportion in the mix. This was recently discussed on the list (check the archives), but in a nutshell make sure you have current info on the new component ratios. See the Ellis paper in Microscopy Today: E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006
I have used her Low Viscosity formula with Quetol-651 and it is a very nice resin in my opinion. Trims and sections VERY nicely and the sections seem very stable in the beam. Stains nicely too. I did not have BDMA so I used DMAE at 0.09g/10g "batch" (according to the Ellis formulas) and it is well polymerized at 16hr -at- 70C (I like a slow set mix). The 0.09g is just 5 drops from a Samco #241 plastic pipet; 4 drops took 2 day to harden, but was good then.
You can use acetone straight through, or ethanol, then change to acetone for infiltration. I don't need to use propylene oxide. There is much excellent work published that didn't use it.
Osmium tetroxide is dangerous - the vapor - but put the waste in a bottle with some vegetable oil and it is reduced and less of a problem to have around until waste pick-up. If there are chemists out there, please tell us the facts, but the reduced osmium product is not much of a concern (but still handle cautiously).
Wikipedia: Osmium in a metallic form is extremely dense, blue-white, brittle, and lustrous even at high temperatures, but proves to be extremely difficult to make. Powdered osmium is easier to make, but powdered osmium exposed to air leads to the formation of osmium tetroxide (OsO4), which is very toxic. The tetroxide is a powerful oxidizing agent, very volatile, water-soluble, pale yellow, crystalline solid with a strong smell that boils at 130°C. By contrast osmium dioxide (OsO2) is black, non-volatile and much less reactive and toxic.
Spurr-replacement "New Formulation" E. Ann Ellis, Microscopy Today, Vol 14, No 4, July 2006
Microscopy Today, July 2006, E. Ann Ellis "Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations"
All formulas are for a "10g batch" - with reference to the (epoxy + acid anhydride)
4.10 5.90 0.95 0.10 Hard 4.10 5.90 1.43 0.10 Standard 4.10 5.90 1.90 0.10 Soft
Low Viscosity Formula (Half the viscosity of the "Refomulated Spurr's") ------------------------- ERL 4221 2.22g Quetol-651 1.40g NSA 6.38g DER-736 1.43g BDMA 0.2g Some think BDMA should be less, 0.1g I (DAC) used 0.09g DMAE (5 drops from a Samco #241 pipet) and it set in 16h -at- 70C
Dale Callaham
sue.tyler-at-noaa.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: sue.tyler-at-noaa.gov } Name: Sue Tyler } } Organization: Cooperative Oxford Laboratory } } Title-Subject: [Filtered] chemical substitution } } Question: I have been asked to research options to substitute Spurr's } epoxy resin kit, propylene oxide and osmium tetroxide for non } hazardous chemicals. } } Has anyone tried and liked Quetol-651 in place of Spurr's? Do you } know of other options that work well for low viscosity epoxy resins? } } Do you like/prefer acetone in place of chloroform? } } Do you like acetonitrile in place of propylene oxide? } } I am relativley new to EM and prefer to use the old standards and am } afraid to try new. Your expert opinions would be greatly appreciated. } } Thanks, } Sue } } Login Host: 205.156.36.37 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Fri Mar 14 09:24:44 2008 } 11, 11 -- Received: from [192.168.50.33] (msdvpn8.msd.anl.gov [130.202.238.72]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EEOggg027011 } 11, 11 -- for {microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 09:24:44 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240802c4003b12f731-at-[192.168.50.33]} } 11, 11 -- Date: Fri, 14 Mar 2008 09:24:43 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: sue.tyler-at-noaa.gov (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: chemical substitution } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 22 -- From dac-at-research.umass.edu Fri Mar 14 15:07:14 2008 17, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2EK7DKR022779 17, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 15:07:14 -0500 17, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 17, 22 -- (authenticated bits=0) 17, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m2EK7DST009293 17, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 17, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Mar 2008 16:07:13 -0400 17, 22 -- Message-ID: {47DAE9F2.1010207-at-research.umass.edu} 17, 22 -- Date: Fri, 14 Mar 2008 16:11:14 -0500 17, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 17, 22 -- Reply-To: dac-at-research.umass.edu 17, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 17, 22 -- MIME-Version: 1.0 17, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 17, 22 -- Subject: Re: [Microscopy] viaWWW: chemical substitution 17, 22 -- References: {200803141435.m2EEZkqc012209-at-ns.microscopy.com} 17, 22 -- In-Reply-To: {200803141435.m2EEZkqc012209-at-ns.microscopy.com} 17, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 22 -- Content-Transfer-Encoding: 8bit 17, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 25, 25 -- From greggps-at-umich.edu Thu Mar 20 08:12:12 2008 25, 25 -- Received: from OWA01.adsroot.itcs.umich.edu (owa01.adsroot.itcs.umich.edu [141.211.27.138]) 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2KDBsQJ011811 25, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 08:12:01 -0500 25, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA01.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); 25, 25 -- Tue, 18 Mar 2008 10:29:25 -0400 25, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 25, 25 -- Content-class: urn:content-classes:message 25, 25 -- MIME-Version: 1.0 25, 25 -- Content-Type: text/plain; 25, 25 -- charset="iso-8859-1" 25, 25 -- Subject: Re: chemical substitution-Osmium safety 25, 25 -- Date: Tue, 18 Mar 2008 10:29:24 -0400 25, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF40270CF90-at-ECLUST2-VS3.adsroot.itcs.umich.edu} 25, 25 -- In-reply-to: {200803142017.m2EKHMA6001873-at-ns.microscopy.com} 25, 25 -- X-MS-Has-Attach: 25, 25 -- X-MS-TNEF-Correlator: 25, 25 -- Thread-topic: Re: chemical substitution-Osmium safety 25, 25 -- Thread-index: AciGEGvT9XU60E86R1mZKpgYCOlr8gC8LQDQ 25, 25 -- References: {200803142017.m2EKHMA6001873-at-ns.microscopy.com} 25, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 25, 25 -- To: {Microscopy-at-microscopy.com} 25, 25 -- X-OriginalArrivalTime: 18 Mar 2008 14:29:25.0125 (UTC) FILETIME=[771BE350:01C88904] 25, 25 -- Content-Transfer-Encoding: 8bit 25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2KDBsQJ011811 ==============================End of - Headers==============================
As an additional caveat, I will add this: Our Office of Environmental Safety does NOT want us to mix the osmium with oil. The company they use to take away our toxic stuff will not take it that way. We just collect it in a 500ml bottle and call them when its full. It has usually "gone black" by then. SO...check with your in-house safety people before you use any method.
Lee
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Dear All I have a JEOL JSM-5600LV Scanning Electron Microscope and I use the Secondary Electron (SE) detector for biological work. Suddenly I have lost the image and in its place a distorted herringbone pattern appears. Whatever I do via the SEM menu the distortion remains, while the controlling PC appears to be working properly. Can any one help by advising as to what may be at fault here? Best regards yorgos
Dr Yorgos Nikas Athens Innovative Microscopy Skra 36 Voula 16673 GREECE eikonika-at-otenet.gr Tel/fax +30 210 8957677 Mobile +30 6945 107477
==============================Original Headers============================== 3, 22 -- From eikonika-at-otenet.gr Thu Mar 20 11:48:14 2008 3, 22 -- Received: from kane.otenet.gr (kane.otenet.gr [195.170.0.77]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2KGmC6D031773 3, 22 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 11:48:13 -0500 3, 22 -- Received: from axeron (athedsl-181747.home.otenet.gr [85.74.29.145]) 3, 22 -- by kane.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id m2KGmBij004533 3, 22 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 18:48:11 +0200 3, 22 -- Message-ID: {000901c88aaa$2fd17a30$2101a8c0-at-axeron} 3, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 3, 22 -- To: {microscopy-at-microscopy.com} 3, 22 -- Subject: Urgent help needed Jeol SEM image distortion 3, 22 -- Date: Thu, 20 Mar 2008 18:48:12 +0200 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: text/plain; 3, 22 -- format=flowed; 3, 22 -- charset="iso-8859-7"; 3, 22 -- reply-type=original 3, 22 -- Content-Transfer-Encoding: 7bit 3, 22 -- X-Priority: 3 3, 22 -- X-MSMail-Priority: Normal 3, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 3, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
Do you have a backscattered electron detector, if so how does this image present?
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {eikonika-at-otenet.gr} To: {protrain-at-emcourses.com} Sent: Thursday, March 20, 2008 4:49 PM
Dear All I have a JEOL JSM-5600LV Scanning Electron Microscope and I use the Secondary Electron (SE) detector for biological work. Suddenly I have lost the image and in its place a distorted herringbone pattern appears. Whatever I do via the SEM menu the distortion remains, while the controlling PC appears to be working properly. Can any one help by advising as to what may be at fault here? Best regards yorgos
Dr Yorgos Nikas Athens Innovative Microscopy Skra 36 Voula 16673 GREECE eikonika-at-otenet.gr Tel/fax +30 210 8957677 Mobile +30 6945 107477
==============================Original Headers============================== 3, 22 -- From eikonika-at-otenet.gr Thu Mar 20 15:22:39 2008 3, 22 -- Received: from kane.otenet.gr (kane.otenet.gr [195.170.0.77]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2KKMcZe006210 3, 22 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 15:22:39 -0500 3, 22 -- Received: from axeron (athedsl-187725.home.otenet.gr [85.74.52.235]) 3, 22 -- by kane.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id m2JJ1csB001154 3, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Mar 2008 21:01:42 +0200 3, 22 -- Message-ID: {001301c889f3$adb026c0$2101a8c0-at-axeron} 3, 22 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 3, 22 -- To: {microscopy-at-microscopy.com} 3, 22 -- Subject: Urgent Help Needed JEOL SEM image distortion 3, 22 -- Date: Wed, 19 Mar 2008 21:01:39 +0200 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: text/plain; 3, 22 -- format=flowed; 3, 22 -- charset="iso-8859-7"; 3, 22 -- reply-type=original 3, 22 -- Content-Transfer-Encoding: 7bit 3, 22 -- X-Priority: 3 3, 22 -- X-MSMail-Priority: Normal 3, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3028 3, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3028 ==============================End of - Headers==============================
Today we just happened to have the service people from JEOL to work on our JEOL JSM-5800LV. I mentioned your problem and it didn't seem to ring a familiar bell with them. They said a service call seems to be needed and the SEM should be checked out with an oscilloscope and other diagnostic tools.
Stu Smalinskas, P.E. Metallurgist SKF USA
--- eikonika-at-otenet.gr wrote: } } Dear All } I have a JEOL JSM-5600LV Scanning Electron } Microscope and I use the } Secondary Electron (SE) detector for biological } work. Suddenly I have lost } the image and in its place a distorted herringbone } pattern appears. } Whatever I do via the SEM menu the distortion } remains, while the controlling } PC appears to be working properly. Can any one help } by advising as to what } may be at fault here? } Best regards } yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } eikonika-at-otenet.gr } Tel/fax +30 210 8957677 } Mobile +30 6945 107477 } }
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This is probably way to too simple, but I like simple solutions and am a hopeless optimist:
Have you inadverently switched the display to Y-modulation? This would result in a "herringbone" pattern on the display CRT. Solution: switch the display to TV or SL modes. It would be shame to have a service call for something this simple, but I will admit that I have come close a couple of times for similar oversights.
If it is something more complex, I recommend that you also reboot the system if that has not been done already. Keep in mind that some glitches in this unit will not reset from a soft reboot. Shut the power off completely and wait a few minutes before restarting the system.
Good luck.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
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I have to replace a couple of cables in our JSM-35C, and I was going to make them myself, which is no big feat for me.
Does anybody know what the name for the small BNC-like connectors is? I can't seem to find them online. I just need to know what the name is so I can order the cable ends.
Thanks,
Justin.
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 5, 27 -- From kraftpiano-at-gmail.com Thu Mar 20 18:54:16 2008 5, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2KNsG1S020526 5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 18:54:16 -0500 5, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so673247rvb.30 5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 16:54:16 -0700 (PDT) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=beta; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=bTvCNgq3GMjdsDwNDXC+3bEFrwbmGvhemjDUUqdx7Sk=; 5, 27 -- b=LKKx1iBIvVzhHaoYs/go/nLgbxNaIqDhPkY9VWEhIUrbOEgxv+3XS7yjQU0y0pn4vsZ/3XZpsm6etJIfn7vpY5mz9sGebyKWLpJHZW0HaawwGdBHDi3VsppfYJ96VJEq6x+XvEa8b65kFsIknvA/PGZlXQ3aMieW0H86dW148DI= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=beta; 5, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=p2r7/FAIhImS79YIc7h0tN2Y4LBEdW37JO3pPA4wdq2Q8leN0z3wVquENnzlpLaCtn5uwt6MnD2/aMg2aCy2X8Lm/enbYomG/wkJT0J/0XYMtftZL/AHRj5CCXKoDdMKwMBWk5SeT2+9xG/SjrSO+fNltzcBvUXwZAgevnaFjl0= 5, 27 -- Received: by 10.141.15.19 with SMTP id s19mr1166165rvi.75.1206057255981; 5, 27 -- Thu, 20 Mar 2008 16:54:15 -0700 (PDT) 5, 27 -- Received: by 10.140.161.11 with HTTP; Thu, 20 Mar 2008 16:54:15 -0700 (PDT) 5, 27 -- Message-ID: {25e2b0d20803201654v37afc14es194437208fb3be2b-at-mail.gmail.com} 5, 27 -- Date: Thu, 20 Mar 2008 18:54:15 -0500 5, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Subject: JEOL connectors. 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 3, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.173]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0836R001151 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 19:08:03 -0500 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=BWxOVqx7df3bWjl8u0trWM8NZbKmo2/jVjRz+52j6CY=; 3, 27 -- b=LKgmS9NYkJGO6ybLDF1cLlKPhU6LIS2aFu8V9IJqA+FlUV/xACyupt0kMCG/Z/JhvFSxp5nZsUfmeDg6jm7sMFdtK3fclLDk5l3LtQvmcMTwyjJ+kwBYYqS8Jtp2GCAH0PIRcq+9cJUenbPj7pwcP+9p9VPEsZIHviJYoVHwk84= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=MG3PAKN5EscmHPVM9cdNoWZNbUySwy4cYXgYX0ZkufFz7V1CosVRXuQB3pHne+Q/Kg9vHCasvVLiSrviGejuOj2oQ+UF4OG8UvF1T17pawDPBARWnTJW5S286wHTy7yPgAGnTvwvNUJLznn6E/7hJ2AIwKDsMKIvloeLCd7oj9U= 3, 27 -- Received: by 10.142.81.6 with SMTP id e6mr1940572wfb.205.1206058082290; 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Message-ID: {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} 3, 27 -- Subject: Double overhead charges 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Are you paying with federal funds? If so, they can't charge you more than they charge their own users. If you are paying with non-federal funds, it could be fair since most EM core facilities are running at a loss and subsidizing the facility for their own users. In this case you aren't really paying overhead but simply a fair market rate.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: marksmsa-at-gmail.com [mailto:marksmsa-at-gmail.com] Sent: Thursday, March 20, 2008 7:09 PM To: Phillips, Thomas E.
One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
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==============================Original Headers============================== 14, 28 -- From PhillipsT-at-missouri.edu Thu Mar 20 19:16:44 2008 14, 28 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0Gih4014155 14, 28 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 20 Mar 2008 19:16:44 -0500 14, 28 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 14, 28 -- Thu, 20 Mar 2008 19:16:43 -0500 14, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 28 -- MIME-Version: 1.0 14, 28 -- Content-Type: text/plain; 14, 28 -- charset="us-ascii" 14, 28 -- x-cr-puzzleid: {EDF79C64-4261-43C6-B727-663B849D5066} 14, 28 -- x-cr-hashedpuzzle: Acnz BkDg CeBs Ega8 FtM5 GCQ9 G6tI LS8P LvWZ Na02 OTYJ Oa2a OzVa SZDL S5Mq S5Wh;2;bQBhAHIAawBzAG0AcwBhAEAAZwBtAGEAaQBsAC4AYwBvAG0AOwBtAGkAYwByAG8AcwBjAG8AcAB5AEAAbQBzAGEALgBtAGkAYwByAG8AcwBjAG8AcAB5AC4AYwBvAG0A;Sosha1_v1;7;{EDF79C64-4261-43C6-B727-663B849D5066};cABoAGkAbABsAGkAcABzAHQAQABtAGkAcwBzAG8AdQByAGkALgBlAGQAdQA=;Fri, 21 Mar 2008 00:15:55 GMT;UgBFADoAIABbAE0AaQBjAHIAbwBzAGMAbwBwAHkAXQAgAEQAbwB1AGIAbABlACAAbwB2AGUAcgBoAGUAYQBkACAAYwBoAGEAcgBnAGUAcwA= 14, 28 -- Content-class: urn:content-classes:message 14, 28 -- Subject: RE: [Microscopy] Double overhead charges 14, 28 -- Date: Thu, 20 Mar 2008 19:15:55 -0500 14, 28 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD03D9E67E-at-UM-XMAIL06.um.umsystem.edu} 14, 28 -- In-Reply-To: {200803210008.m2L08ogA002418-at-ns.microscopy.com} 14, 28 -- X-MS-Has-Attach: 14, 28 -- X-MS-TNEF-Correlator: 14, 28 -- Thread-Topic: [Microscopy] Double overhead charges 14, 28 -- Thread-Index: AciK58W97qnIXxSzRRmL00JfaB+rPgAAJdUg 14, 28 -- References: {200803210008.m2L08ogA002418-at-ns.microscopy.com} 14, 28 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 14, 28 -- To: {marksmsa-at-gmail.com} 14, 28 -- Cc: {Microscopy-at-msa.microscopy.com} 14, 28 -- X-OriginalArrivalTime: 21 Mar 2008 00:16:43.0701 (UTC) FILETIME=[D7C35E50:01C88AE8] 14, 28 -- Content-Transfer-Encoding: 8bit 14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2L0Gih4014155 ==============================End of - Headers==============================
What do they look like? They could be SMA or Fischer. BNC are larger than either of these.
gary g.
At 04:56 PM 3/20/2008, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Thu Mar 20 20:11:29 2008 9, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2L1BTDe028445 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 20:11:29 -0500 9, 20 -- Message-Id: {200803210111.m2L1BTDe028445-at-ns.microscopy.com} 9, 20 -- Received: (qmail 5245 invoked from network); 20 Mar 2008 18:10:23 -0700 9, 20 -- Received: by simscan 1.1.0 ppid: 5239, pid: 5243, t: 0.1036s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by qsmtp4 with SMTP; 20 Mar 2008 18:10:23 -0700 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Thu, 20 Mar 2008 18:11:26 -0700 9, 20 -- To: kraftpiano-at-gmail.com 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] JEOL connectors. 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200803202356.m2KNui3j023218-at-ns.microscopy.com} 9, 20 -- References: {200803202356.m2KNui3j023218-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-6E8A15BE ==============================End of - Headers==============================
Sorry for the multiple messages, but I received a good number of responses that indicated that this connector must be seen to be identified. Here's a link to the photos:
http://www.jkraft.net/jeol.html
Thanks again for the assistance.
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
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Dear Dr. Marks- I charge a 70% overhead rate here, since that is what the NIH rate for my institution is. I know that other facilities here in NYC also have overhead "surcharges" for users from outside their institutions...I know of one that simply doubles its in-house fees. I also know of one institution that heavily subsidizes it in-house facilities and does not allow outsiders access. I did not institute this fee out of malice or willy-nilly, but rather at the direction of our office of Research and Sponsored Programs. I do understand the extra financial burden it places. I give full disclosure (as did the other university you consulted) and leave it to the client to decide about how/if to proceed.
Lee
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-- Leona Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
Justin, Actually, if you look carefully, there are quite a few differences between BNC and MB (miniature bayonet) connectors, like the male/female combinations. Anyway, search for miniature bayonet or type MB coaxial connectors. Make sure you get them for the correct cable size (diameter).
There exists a type called miniature BNC which is, in fact, identical to a BNC only smaller. It is not compatible with type MB connectors.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Thursday, March 20, 2008 9:58 PM To: kenconverse-at-qualityimages.biz
Sorry for the multiple messages, but I received a good number of responses that indicated that this connector must be seen to be identified. Here's a link to the photos:
http://www.jkraft.net/jeol.html
Thanks again for the assistance.
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
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==============================Original Headers============================== 18, 26 -- From kenconverse-at-qualityimages.biz Fri Mar 21 08:17:56 2008 18, 26 -- Received: from mta10.adelphia.net (mta10.adelphia.net [68.168.78.202]) 18, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LDHtCL027969 18, 26 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 21 Mar 2008 08:17:56 -0500 18, 26 -- Received: from Ken ([72.227.100.25]) by mta10.adelphia.net 18, 26 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 18, 26 -- id {20080321131757.QESM10451.mta10.adelphia.net-at-Ken} ; 18, 26 -- Fri, 21 Mar 2008 09:17:57 -0400 18, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 26 -- To: {kraftpiano-at-gmail.com} 18, 26 -- Cc: "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 18, 26 -- Subject: RE: [Microscopy] Clarification on JEOL connector types. 18, 26 -- Date: Fri, 21 Mar 2008 09:17:45 -0400 18, 26 -- Message-ID: {000801c88b55$f43e1710$6401a8c0-at-Ken} 18, 26 -- MIME-Version: 1.0 18, 26 -- Content-Type: text/plain; 18, 26 -- charset="us-ascii" 18, 26 -- X-Priority: 3 (Normal) 18, 26 -- X-MSMail-Priority: Normal 18, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 18, 26 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 18, 26 -- Importance: Normal 18, 26 -- Thread-Index: AciK9wLizjeQQ2ZNQdGD/yIYEYL8RAAXgigg 18, 26 -- In-Reply-To: {200803210158.m2L1wARf014666-at-ns.microscopy.com} 18, 26 -- Content-Transfer-Encoding: 8bit 18, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2LDHtCL027969 ==============================End of - Headers==============================
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Email: arnec-at-bio.umass.edu Name: Arne Christensen
Organization: UMass, Amherst
Title-Subject: [Filtered] Lipid raft markers in fixed tissue
Question: Can anyone recommend a marker for lipid rafts that will work on fixed tissue. I prefer a stain, or monoclonal antibody.
I have thus far found a monoclonal anti-GM1 and anti-SGC, in addition to a fluoraphore conjugated beta subunit of the choleratoxin. However, I have not yet found very nice published data of these used on fixed tissue.
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Organization: Arizona State University
Title-Subject: [Filtered] cryogens for rapid freezing at ambient pressure
Question: in the past our lab has used liquid propane as a cryogen for ambient-pressure plunge-freezing methods. Due to concerns about the hazards associated with openly handling propane during freezing and the need to store pressurized cannisters of propane on-site, we have begun looking into alternative, less hazardous cryogens to use for plunge-freezing. In particular, certain HCFCs such as DuPont's SUVA-124 appear promising. Does anyone in the community have experience using HCFCs or possibly other compounds for this technique? Thank you,
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I wonder if there is a good image analysis tool or method that can measure glass/carbon fiber (those found in polymers as fillers) length automatically. Typically we need 300+ data points/sample for statistical analysis. We use a semi-auto method and only the data log-in portion is automatic.
My understanding is that overlapping of fibers (which is unavoidable) makes automated measurements difficult. I'd like to ask for your suggestion/advice. Thank you very much!
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Email: etobrien-at-email.unc.edu Name: Tim O'Brien
Organization: UNC Chapel Hill
Title-Subject: [Filtered] Forces in Microscopy Course
Question: Persons interested in applying or assessing forces on cells, proteins DNA, and so on, may be interested in our fourth annual FORCE MEASUREMENT AND MANIPULATION IN BIOLOGICAL MICROSCOPY course held at UNC Chapel Hill from May 27-30th. We offer hands-on experience with combined AFM-fluorescence microscopy, magnetic and laser tweezers, and microfluidics, plus morning lectures on how to understand forces at the nm scale. Please google CISMM or go direclty to http://www.cs.unc.edu/cismm/ForcesWorkshop for more information!
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Email: sergequark-at-aol.com Name: meyer
Organization: Fudan university
Title-Subject: [Filtered] Keratin embedded in epon 812 resin, size reduction
Question: Hello,
I work on avian feathers and TEM pictures are required. I hear that technique including microscale, permit to mesure the size reduction of the keratin layer of the samples, embedded in keratin.
Could somebody can tell me some reference articles about this subject.
Folks- It's still early in the morning for me and perhaps I've got this wrong, but it appears that the main problem that Prof. Marks had was with his OWN university charging him overhead to use someone a TEM elsewhere. All EM is expensive and every university of which I've heard charges some form of overhead- usually more for outsiders and industry. However, charging it to use facilities they don't support....that does seem a bit like extortion to me. Years ago, I had occasion to use TEMs at a second university due to similar equipment needs that my school couldn't provide. I don't recall my home school charging our accounts to use off-site resources. John
} [Original Message] } From: {lcgould-at-med.cornell.edu} } To: {jwheckman-at-earthlink.net} } Date: 3/21/2008 6:13:33 AM } Subject: [Microscopy] Re: Double overhead charges } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Dr. Marks- } I charge a 70% overhead rate here, since that is what the NIH rate } for my institution is. I know that other facilities here in NYC also } have overhead "surcharges" for users from outside their } institutions...I know of one that simply doubles its in-house fees. } I also know of one institution that heavily subsidizes it in-house } facilities and does not allow outsiders access. } I did not institute this fee out of malice or willy-nilly, but rather } at the direction of our office of Research and Sponsored Programs. } I do understand the extra financial burden it places. I give full } disclosure (as did the other university you consulted) and leave it } to the client to decide about how/if to proceed. } } Lee } } } } } --------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------------- - } } } } One of my students would like to use a TEM at another university since } } we don't have an appropriate instrument. The other university charges } } $50/hr and (for their own faculty) they have an overhead rate of 50% } } on research expenses. They are requesting that I pay $75/hr (i.e. they } } will add overhead) and then my university wants to tack on their own } } overhead rate (50%) as well. } } } } I consider this unethical. I wonder if anyone has come across this } } before and has found a useful resolution beyond going bankrupt. } } } } -- } } Professor Laurence Marks } } Department of Materials Science and Engineering } } MSE Rm 2036 Cook Hall } } 2220 N Campus Drive } } Northwestern University } } Evanston, IL 60208, USA } } Tel: (847) 491-3996 Fax: (847) 491-7820 } } email: L-marks at northwestern dot edu } } Web: www.numis.northwestern.edu } } EMM2007 http://ns.crys.ras.ru/EMMM07/ } } Commission on Electron Diffraction of IUCR } } www.numis.northwestern.edu/IUCR_CED } } } } ==============================Original Headers============================== } } 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 } } 3, 27 -- Received: from wf-out-1314.google.com } } (wf-out-1314.google.com [209.85.200.173]) } } 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m2L0836R001151 } } 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 } } 19:08:03 -0500 } } 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 } } 3, 27 -- for {Microscopy-at-microscopy.com} ; 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} } 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) } } 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 } } 17:08:02 -0700 (PDT) } } 3, 27 -- Message-ID: } } {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} } } 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 } } 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} } } 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} } } 3, 27 -- Subject: Double overhead charges } } 3, 27 -- MIME-Version: 1.0 } } 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } } 3, 27 -- Content-Transfer-Encoding: 7bit } } 3, 27 -- Content-Disposition: inline } } ==============================End of - Headers============================== } } } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate in Biochemistry and } Cell & Developmental Biology } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Weill Cornell Medical College } } voice (212)746-6146 } fax (212)746-8175 } http://www.cornellcelldevbiology.org } http://www.cornellbiochem.org } } ==============================Original Headers============================== } 8, 25 -- From lcgould-at-med.cornell.edu Fri Mar 21 08:06:13 2008 } 8, 25 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LD6Dk6015268 } 8, 25 -- for {microscopy-at-microscopy.com} ; 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Folks- I may have this wrong, but it seemed to me that Prof Marks real problem was with his OWN university charging him overhead to use off-site TEM facilities. All EM is expensive to maintain and universities of which I've dealt with have some form of overhead charges- usually more for outsides and industry. However, a university charging overhead to use off-site TEM facilities that one's university doesn't support does seem a bit like over-extortion to me. I had a similar occasion, years ago, when my university couldn't provide the TEM equipment I needed and I used the outstanding facilities at a near-by university. It wasn't cheap but I don't recall our university charging anything additional to the accounts. John
} [Original Message] } From: {lcgould-at-med.cornell.edu} } To: {jwheckman-at-earthlink.net} } Date: 3/21/2008 6:13:33 AM } Subject: [Microscopy] Re: Double overhead charges } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Dr. Marks- } I charge a 70% overhead rate here, since that is what the NIH rate } for my institution is. I know that other facilities here in NYC also } have overhead "surcharges" for users from outside their } institutions...I know of one that simply doubles its in-house fees. } I also know of one institution that heavily subsidizes it in-house } facilities and does not allow outsiders access. } I did not institute this fee out of malice or willy-nilly, but rather } at the direction of our office of Research and Sponsored Programs. } I do understand the extra financial burden it places. I give full } disclosure (as did the other university you consulted) and leave it } to the client to decide about how/if to proceed. } } Lee } } } } } --------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------------- - } } } } One of my students would like to use a TEM at another university since } } we don't have an appropriate instrument. The other university charges } } $50/hr and (for their own faculty) they have an overhead rate of 50% } } on research expenses. They are requesting that I pay $75/hr (i.e. they } } will add overhead) and then my university wants to tack on their own } } overhead rate (50%) as well. } } } } I consider this unethical. I wonder if anyone has come across this } } before and has found a useful resolution beyond going bankrupt. } } } } -- } } Professor Laurence Marks } } Department of Materials Science and Engineering } } MSE Rm 2036 Cook Hall } } 2220 N Campus Drive } } Northwestern University } } Evanston, IL 60208, USA } } Tel: (847) 491-3996 Fax: (847) 491-7820 } } email: L-marks at northwestern dot edu } } Web: www.numis.northwestern.edu } } EMM2007 http://ns.crys.ras.ru/EMMM07/ } } Commission on Electron Diffraction of IUCR } } www.numis.northwestern.edu/IUCR_CED } } } } ==============================Original Headers============================== } } 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 } } 3, 27 -- Received: from wf-out-1314.google.com } } (wf-out-1314.google.com [209.85.200.173]) } } 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m2L0836R001151 } } 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 } } 19:08:03 -0500 } } 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 } } 3, 27 -- for {Microscopy-at-microscopy.com} ; 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} } 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) } } 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 } } 17:08:02 -0700 (PDT) } } 3, 27 -- Message-ID: } } {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} } } 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 } } 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} } } 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} } } 3, 27 -- Subject: Double overhead charges } } 3, 27 -- MIME-Version: 1.0 } } 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } } 3, 27 -- Content-Transfer-Encoding: 7bit } } 3, 27 -- Content-Disposition: inline } } ==============================End of - Headers============================== } } } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate in Biochemistry and } Cell & Developmental Biology } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Weill Cornell Medical College } } voice (212)746-6146 } fax (212)746-8175 } http://www.cornellcelldevbiology.org } http://www.cornellbiochem.org } } ==============================Original Headers============================== } 8, 25 -- From lcgould-at-med.cornell.edu Fri Mar 21 08:06:13 2008 } 8, 25 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LD6Dk6015268 } 8, 25 -- for {microscopy-at-microscopy.com} ; 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Thanks to the replies. I learned that the connectors are called KM connectors, and after some brief searching, I found out that they are primarily used in commercial aircraft aviation systems. (Someone did comment to me that the JSM-35 I have looks like an airplane cockpit!)
Anyway, for those who might be interested, here is a manufacturer I found for the connectors, complete with mechanical drawings and specs:
I am assuming that the funds to pay for the TEM time are coming from a grant. You are purchasing a service from another vendor (the other university) and your university will charge overhead on all non-equipment purchases.
Your service provider just happens to be a different university and this other university will charge overhead on all income coming in, just like your university. If this other university did not, you would be getting a cheaper rate than the in-house users of the TEM at the other university would. Remember the in-house users are paying $50/hr + overhead.
Mike
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One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 3, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.173]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0836R001151 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 19:08:03 -0500 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=BWxOVqx7df3bWjl8u0trWM8NZbKmo2/jVjRz+52j6CY=; 3, 27 -- b=LKgmS9NYkJGO6ybLDF1cLlKPhU6LIS2aFu8V9IJqA+FlUV/xACyupt0kMCG/Z/JhvFSxp5nZsUfmeDg6jm7sMFdtK3fclLDk5l3LtQvmcMTwyjJ+kwBYYqS8Jtp2GCAH0PIRcq+9cJUenbPj7pwcP+9p9VPEsZIHviJYoVHwk84= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=MG3PAKN5EscmHPVM9cdNoWZNbUySwy4cYXgYX0ZkufFz7V1CosVRXuQB3pHne+Q/Kg9vHCasvVLiSrviGejuOj2oQ+UF4OG8UvF1T17pawDPBARWnTJW5S286wHTy7yPgAGnTvwvNUJLznn6E/7hJ2AIwKDsMKIvloeLCd7oj9U= 3, 27 -- Received: by 10.142.81.6 with SMTP id e6mr1940572wfb.205.1206058082290; 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Message-ID: {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} 3, 27 -- Subject: Double overhead charges 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From mdunlap-at-ucmerced.edu Fri Mar 21 10:13:26 2008 10, 24 -- Received: from mario.ucmerced.edu (mario.ucmerced.edu [169.236.10.223]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LFDPXQ012725 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:13:26 -0500 10, 24 -- Received: from engwin.ucmerced.edu ([169.236.142.218]) 10, 24 -- (authenticated bits=0) 10, 24 -- by mario.ucmerced.edu (8.12.10/8.12.9) with ESMTP id m2LFDNY0023221 10, 24 -- (version=TLSv1/SSLv3 cipher=EDH-RSA-DES-CBC3-SHA bits=168 verify=NOT) 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 07:13:23 -0800 (PST) 10, 24 -- From: "Mike Dunlap" {mdunlap-at-ucmerced.edu} 10, 24 -- Reply-To: "mdunlap-at-ucmerced.edu" {mdunlap-at-ucmerced.edu} 10, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 24 -- Subject: RE: [Microscopy] Double overhead charges 10, 24 -- Date: Fri, 21 Mar 2008 08:13:19 -0700 10, 24 -- Organization: UC Merced 10, 24 -- Message-ID: {20080321081319559.00000003068-at-mdunlap} 10, 24 -- In-Reply-To: {200803210011.m2L0B7rO006648-at-ns.microscopy.com} 10, 24 -- References: {200803210011.m2L0B7rO006648-at-ns.microscopy.com} 10, 24 -- X-Mailer: Oracle Connector for Outlook 10.1.2.0.7 80425 (11.0.8206) 10, 24 -- X-Accept-Language: en-us, en 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=us-ascii 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2LFDPXQ012725 ==============================End of - Headers==============================
The Astromaterials Research and Exploration Science Directorate (ARES) at the NASA Johnson Space Center is accepting applications for a post-doctoral position in field-emission analytical STEM of natural nanomaterials from cometary dust particles, meteorites and lunar samples. This position provides an opportunity for a candidate with experience in STEM-based EELS and EDX compositional spectrum imaging and HAADF-imaging techniques to apply these techniques to unique and challenging problems in space science.
The candidate will have full access to a JEOL 2500SE 200kV field emission STEM equipped with a Gatan Tridiem GIF, slow scan CCD cameras and a thin window EDX detector and a JEOL 2000FX STEM also with CCD cameras and EDX. Sample preparation facilities include ultramicrotomes, ion mills and a new FEI Quanta 3D 600 dual-beam FIB/SEM that will be installed this summer.
The preferred qualifications for this position are a PhD in Geology/Planetary Science and experience in advanced TEM techniques, although candidates with a PhD and the requisite TEM expertise within the fields of materials science or solid state chemistry, and who demonstrate a motivation to work in the planetary science field, are also encouraged to apply. The candidate should have strong computer and communication (written and oral) skills and work well with others in a research group setting.
The position will be administered by the National Research Council or the Lunar and Planetary Institute and will be for 1 year with the possibility of renewal for 2 additional years.
Interested applicants should submit their vitae via email to: Dr. Lindsay Keller, Lindsay.P.Keller-at-nasa.gov.
==============================Original Headers============================== 7, 27 -- From roy.christoffersen-1-at-nasa.gov Fri Mar 21 10:34:12 2008 7, 27 -- Received: from ndjsbar02.ndc.nasa.gov (ndjsbar02.ndc.nasa.gov [198.120.25.39]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LFYBOf026253 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:34:11 -0500 7, 27 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) 7, 27 -- by ndjsbar02.ndc.nasa.gov (Spam Firewall) with ESMTP id 1ED876AB0F9 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:34:11 -0500 (CDT) 7, 27 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) by ndjsbar02.ndc.nasa.gov with ESMTP id tH15Hig8nFkApYES for {microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:34:11 -0500 (CDT) 7, 27 -- Received: from NDJSEVS22A.ndc.nasa.gov ([129.166.32.220]) by ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.1830); 7, 27 -- Fri, 21 Mar 2008 10:34:11 -0500 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- Subject: Postdoctoral Position: Analytical TEM of Astromaterials 7, 27 -- Date: Fri, 21 Mar 2008 10:34:10 -0500 7, 27 -- Message-ID: {0E899C0EBD04EF4FA965E0D375B2D4FB01FF3336-at-NDJSEVS22A.ndc.nasa.gov} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Postdoctoral Position: Analytical TEM of Astromaterials 7, 27 -- Thread-Index: AciLaQIe+tJzZmJXSKuoQ/dt85lBjQ== 7, 27 -- From: "Christoffersen, Roy (JSC-KR)[SAIC]" {roy.christoffersen-1-at-nasa.gov} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 21 Mar 2008 15:34:11.0091 (UTC) FILETIME=[02907E30:01C88B69] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2LFYBOf026253 ==============================End of - Headers==============================
First, I don't know how much recourse you will actually have. The powers-that-be may have their policies set. I don't know how much TEM time you are talking about. It may not be a large enough amount for them to consider changing the policy.
The situation is a little worse than double dipping for overhead. If you spend $50 on beam time for one hour, the other university will tack on an extra $25 for overhead and send you a bill for $75. You university will add on their 50% for another $37.50. So you got $50 of beam time and spent $62.50 in indirect costs. That is 125% overhead!
Maybe your funding agency could weigh in on that. It certainly cuts into the amount of work they can get done.
In principle it does not seem fair for a unit to collect overhead on work that is not being done at their site. Years ago, our university was the lead institution on a contract. We subcontracted a portion of the work to another university. As I recall, that portion of the funds was free from our university's overhead. This might be considered to be similar.
Warren
-----Original Message----- X-from: jwheckman-at-earthlink.net [mailto:jwheckman-at-earthlink.net] Sent: Friday, March 21, 2008 8:47 AM To: wesaia-at-iastate.edu
Hi Arne,
Sorry that I don't have a solution but can only tell you I have found the same problem. I have tried an antibody (mouse anti-GM1) that has not worked and also tried biotinylated choleratoxin B subunit with subsequent streptavidin gold. This also hasn't worked. I also can't find good published data showing labeling of fixed tissue sections. I have tried light and TEM level labeling of lightly fixed cryothin sections of cells and tissues without success.
Robert Underwood University of Washington Dermatology
On Fri, 21 Mar 2008 arnec-at-bio.umass.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both arnec-at-bio.umass.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: arnec-at-bio.umass.edu } Name: Arne Christensen } } Organization: UMass, Amherst } } Title-Subject: [Filtered] Lipid raft markers in fixed tissue } } Question: Can anyone recommend a marker for lipid rafts that will work on fixed tissue. I prefer a stain, or monoclonal antibody. } } I have thus far found a monoclonal anti-GM1 and anti-SGC, in addition to a fluoraphore conjugated beta subunit of the choleratoxin. However, I have not yet found very nice published data of these used on fixed tissue. } } Thank you, } Sincerely, } Arne } } } Login Host: 159.189.36.78 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Fri Mar 21 08:24:04 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LDO3Yv003054 } 9, 11 -- for {microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 08:24:04 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240803c409676f42ec-at-[206.69.208.22]} } 9, 11 -- Date: Fri, 21 Mar 2008 08:24:12 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: arnec-at-bio.umass.edu (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Lipid raft markers in fixed tissue } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 22 -- From underwoo-at-u.washington.edu Fri Mar 21 12:24:20 2008 9, 22 -- Received: from mxout4.cac.washington.edu (mxout4.cac.washington.edu [140.142.33.19]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LHOJCu023723 9, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 21 Mar 2008 12:24:20 -0500 9, 22 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103]) 9, 22 -- by mxout4.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m2LHOJKC024542 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 9, 22 -- Fri, 21 Mar 2008 10:24:19 -0700 9, 22 -- Received: from localhost (localhost [127.0.0.1]) 9, 22 -- by hymn13.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m2LHOJEO003886; 9, 22 -- Fri, 21 Mar 2008 10:24:19 -0700 9, 22 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Fri, 21 Mar 2008 10:24:19 PDT 9, 22 -- Date: Fri, 21 Mar 2008 10:24:19 -0700 (PDT) 9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 9, 22 -- To: arnec-at-bio.umass.edu, Microscopy List {Microscopy-at-MSA.Microscopy.Com} 9, 22 -- Subject: [Microscopy] viaWWW: Re:Lipid raft markers in fixed tissue 9, 22 -- In-Reply-To: {200803211326.m2LDQgmi009807-at-ns.microscopy.com} 9, 22 -- Message-ID: {Pine.LNX.4.43.0803211024190.9811-at-hymn13.u.washington.edu} 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 22 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.21.101130 9, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='SUPERLONG_LINE 0.05, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' ==============================End of - Headers==============================
Hello, I am currently using an Edwards S150B sputter coater (1981). The pressure control valve has been compromised and I would like to replace the unit. I need some suggestion about brands with pros and cons. Thanks Teresa
-- Teresa Sawyer Senior Faculty Research Assistant Electron Microscopy Facility Manager Botany and Plant Pathology 2082 Cordely Hall Corvallis OR 97331-2902 541-737-5645 541-737-2332 Fax:541-737-3573
==============================Original Headers============================== 3, 27 -- From sawyert-at-science.oregonstate.edu Sat Mar 22 12:51:42 2008 3, 27 -- Received: from smtp6.oregonstate.edu (smtp6.oregonstate.edu [128.193.15.46]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2MHpgZw029530 3, 27 -- for {Microscopy-at-Microscopy.Com} ; Sat, 22 Mar 2008 12:51:42 -0500 3, 27 -- Received: from localhost (localhost [127.0.0.1]) 3, 27 -- by smtp6.oregonstate.edu (Postfix) with ESMTP id B5B28C8852D 3, 27 -- for {Microscopy-at-Microscopy.Com} ; Sat, 22 Mar 2008 10:51:41 -0700 (PDT) 3, 27 -- X-Virus-Scanned: amavisd-new at oregonstate.edu 3, 27 -- Received: from smtp6.oregonstate.edu ([127.0.0.1]) 3, 27 -- by localhost (smtp.oregonstate.edu [127.0.0.1]) (amavisd-new, port 10024) 3, 27 -- with ESMTP id KQzkUnJsi+5q for {Microscopy-at-Microscopy.Com} ; 3, 27 -- Sat, 22 Mar 2008 10:51:41 -0700 (PDT) 3, 27 -- Received: from frontend.science.oregonstate.edu (mail.science.oregonstate.edu [128.193.224.69]) 3, 27 -- by smtp6.oregonstate.edu (Postfix) with ESMTP id 970E0C88529 3, 27 -- for {Microscopy-at-Microscopy.Com} ; Sat, 22 Mar 2008 10:51:41 -0700 (PDT) 3, 27 -- Received: from [128.193.225.119] (128-193-225-119.science.oregonstate.edu [128.193.225.119]) 3, 27 -- by frontend.science.oregonstate.edu (Postfix) with ESMTP id 882F7B3C051 3, 27 -- for {Microscopy-at-Microscopy.Com} ; Sat, 22 Mar 2008 10:51:41 -0700 (PDT) 3, 27 -- Message-ID: {47E5472D.1070605-at-science.oregonstate.edu} 3, 27 -- Date: Sat, 22 Mar 2008 10:51:41 -0700 3, 27 -- From: Teresa Sawyer {sawyert-at-science.oregonstate.edu} 3, 27 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 3, 27 -- MIME-Version: 1.0 3, 27 -- To: Microscopy Listserv {Microscopy-at-Microscopy.Com} 3, 27 -- Subject: sputter coater 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
M3S will hold its first meeting of 2008, Near Zero: High-Resolution Imaging at Low Temperatures, this Friday, March 28th, at Northwestern University in Evanston, IL. Registration and program information can be found on our website Meetings page;
www.midwestmicroscopy.org
Please do not reply to this posting regarding registration. We look forward to seeing you there.
Elaine Schumacher Program Coordinator Midwest Microscopy and Microanalysis Society elaine-at-midwestmicroscopy.org
==============================Original Headers============================== 8, 23 -- From eschumacher-at-mccrone.com Mon Mar 24 10:12:49 2008 8, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2OFCmnM009362 8, 23 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2008 10:12:49 -0500 8, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Mon, 24 Mar 2008 10:12:46 -0500 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Subject: Meeting: Midwest Microscopy and Microanalysis Society 8, 23 -- Date: Mon, 24 Mar 2008 10:12:45 -0500 8, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DD76-at-MCCRONEMSG.tmg.mccrone.com} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Meeting: Midwest Microscopy and Microanalysis Society 8, 23 -- Thread-Index: AciNwYNOAteM21LhS1qYZ31bux0nLg== 8, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 24 Mar 2008 15:12:46.0096 (UTC) FILETIME=[83E31100:01C88DC1] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2OFCmnM009362 ==============================End of - Headers==============================
I don't think this is correct. Now it has been some time since I was a graduate student and since I was on a university faculty, but we never paid an overhead charge on a purchase from a vendor. When the grant was awarded, there was an overhead charge on that that was pretty hefty and that percentage varies from university to university. To me, what is unethical for Prof. Marks' university is to charge overhead charges on grant monies that has already been charged the university's overhead charges. That is nothing more than double taxation.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mdunlap-at-ucmerced.edu [mailto:mdunlap-at-ucmerced.edu] Sent: Friday, March 21, 2008 8:19 AM To: Walck-at-SouthBayTech.com
I am assuming that the funds to pay for the TEM time are coming from a grant. You are purchasing a service from another vendor (the other university) and your university will charge overhead on all non-equipment purchases.
Your service provider just happens to be a different university and this other university will charge overhead on all income coming in, just like your university. If this other university did not, you would be getting a cheaper rate than the in-house users of the TEM at the other university would. Remember the in-house users are paying $50/hr + overhead.
Mike
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 3, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.173]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0836R001151 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 19:08:03 -0500 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=BWxOVqx7df3bWjl8u0trWM8NZbKmo2/jVjRz+52j6CY=; 3, 27 -- b=LKgmS9NYkJGO6ybLDF1cLlKPhU6LIS2aFu8V9IJqA+FlUV/xACyupt0kMCG/Z/JhvFSxp5nZsU fmeDg6jm7sMFdtK3fclLDk5l3LtQvmcMTwyjJ+kwBYYqS8Jtp2GCAH0PIRcq+9cJUenbPj7pwcP+ 9p9VPEsZIHviJYoVHwk84= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer -encoding:content-disposition; 3, 27 -- b=MG3PAKN5EscmHPVM9cdNoWZNbUySwy4cYXgYX0ZkufFz7V1CosVRXuQB3pHne+Q/Kg9vHCasvV LiSrviGejuOj2oQ+UF4OG8UvF1T17pawDPBARWnTJW5S286wHTy7yPgAGnTvwvNUJLznn6E/7hJ2 AIwKDsMKIvloeLCd7oj9U= 3, 27 -- Received: by 10.142.81.6 with SMTP id e6mr1940572wfb.205.1206058082290; 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Message-ID: {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} 3, 27 -- Subject: Double overhead charges 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From mdunlap-at-ucmerced.edu Fri Mar 21 10:13:26 2008 10, 24 -- Received: from mario.ucmerced.edu (mario.ucmerced.edu [169.236.10.223]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LFDPXQ012725 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:13:26 -0500 10, 24 -- Received: from engwin.ucmerced.edu ([169.236.142.218]) 10, 24 -- (authenticated bits=0) 10, 24 -- by mario.ucmerced.edu (8.12.10/8.12.9) with ESMTP id m2LFDNY0023221 10, 24 -- (version=TLSv1/SSLv3 cipher=EDH-RSA-DES-CBC3-SHA bits=168 verify=NOT) 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 07:13:23 -0800 (PST) 10, 24 -- From: "Mike Dunlap" {mdunlap-at-ucmerced.edu} 10, 24 -- Reply-To: "mdunlap-at-ucmerced.edu" {mdunlap-at-ucmerced.edu} 10, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 24 -- Subject: RE: [Microscopy] Double overhead charges 10, 24 -- Date: Fri, 21 Mar 2008 08:13:19 -0700 10, 24 -- Organization: UC Merced 10, 24 -- Message-ID: {20080321081319559.00000003068-at-mdunlap} 10, 24 -- In-Reply-To: {200803210011.m2L0B7rO006648-at-ns.microscopy.com} 10, 24 -- References: {200803210011.m2L0B7rO006648-at-ns.microscopy.com} 10, 24 -- X-Mailer: Oracle Connector for Outlook 10.1.2.0.7 80425 (11.0.8206) 10, 24 -- X-Accept-Language: en-us, en 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=us-ascii 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2LFDPXQ012725 ==============================End of - Headers==============================
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==============================Original Headers============================== 21, 22 -- From walck-at-southbaytech.com Mon Mar 24 11:49:36 2008 21, 22 -- Received: from flpi195.prodigy.net (flpi195.sbcis.sbc.com [207.115.20.197]) 21, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2OGnaZR026642 21, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Mar 2008 11:49:36 -0500 21, 22 -- X-ORBL: [99.154.21.201] 21, 22 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 21, 22 -- by flpi195.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id m2OGnX6H023625; 21, 22 -- Mon, 24 Mar 2008 09:49:34 -0700 21, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 21, 22 -- To: {Microscopy-at-microscopy.com} 21, 22 -- Cc: {mdunlap-at-ucmerced.edu} 21, 22 -- Subject: RE: [Microscopy] RE: Double overhead charges 21, 22 -- Date: Mon, 24 Mar 2008 09:49:46 -0700 21, 22 -- Message-ID: {003601c88dcf$130e2560$7801a8c0-at-dynamicbl8uno3} 21, 22 -- MIME-Version: 1.0 21, 22 -- Content-Type: text/plain; 21, 22 -- charset="windows-1250" 21, 22 -- Content-Transfer-Encoding: 7bit 21, 22 -- X-Mailer: Microsoft Office Outlook 11 21, 22 -- In-Reply-To: {200803211518.m2LFInho018237-at-ns.microscopy.com} 21, 22 -- thread-index: AciLZt/vjGJ72PBRRjCBIK7G2mCWdwCZ2rQw 21, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Scott - You are confused in your interpretation of how overhead works. Overhead is charged on all purchases from a vendor. The bill (both the direct costs of the item and the indirect costs of overhead) to the funding agency occurs when purchases are made, not when the grant is awarded up front. Faculty sometimes think in those terms but we all realize it doesn't work that way when we get to the end of a grant and/or want to rebudget money from one category to another.
The double overhead in this case is to another school. If it costs X amount for my school to process a grant and pay for heat and electricity in my lab, then it is appropriate for them to charge overhead. If I buy an item from a vendor, they also sometimes feel it necessary to bill me for the true cost of the item to themselves plus enough to pay their own accountants, mailroom staff, webdevelopers, and technical directors (that is their overhead and it is built into the cost). So if Prof. Marks wants to use the services at two universities, isn't this equivalent to him using twice the resources?
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Monday, March 24, 2008 11:51 AM To: Phillips, Thomas E.
I don't think this is correct. Now it has been some time since I was a graduate student and since I was on a university faculty, but we never paid an overhead charge on a purchase from a vendor. When the grant was awarded, there was an overhead charge on that that was pretty hefty and that percentage varies from university to university. To me, what is unethical for Prof. Marks' university is to charge overhead charges on grant monies that has already been charged the university's overhead charges. That is nothing more than double taxation.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mdunlap-at-ucmerced.edu [mailto:mdunlap-at-ucmerced.edu] Sent: Friday, March 21, 2008 8:19 AM To: Walck-at-SouthBayTech.com
I am assuming that the funds to pay for the TEM time are coming from a grant. You are purchasing a service from another vendor (the other university) and your university will charge overhead on all non-equipment purchases.
Your service provider just happens to be a different university and this other university will charge overhead on all income coming in, just like your university. If this other university did not, you would be getting a cheaper rate than the in-house users of the TEM at the other university would. Remember the in-house users are paying $50/hr + overhead.
Mike
------------------------------------------------------------------------ ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 3, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.173]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0836R001151 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 19:08:03 -0500 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=BWxOVqx7df3bWjl8u0trWM8NZbKmo2/jVjRz+52j6CY=; 3, 27 -- b=LKgmS9NYkJGO6ybLDF1cLlKPhU6LIS2aFu8V9IJqA+FlUV/xACyupt0kMCG/Z/JhvFSxp5 nZsU fmeDg6jm7sMFdtK3fclLDk5l3LtQvmcMTwyjJ+kwBYYqS8Jtp2GCAH0PIRcq+9cJUenbPj7p wcP+ 9p9VPEsZIHviJYoVHwk84= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer -encoding:content-disposition; 3, 27 -- b=MG3PAKN5EscmHPVM9cdNoWZNbUySwy4cYXgYX0ZkufFz7V1CosVRXuQB3pHne+Q/Kg9vHC asvV LiSrviGejuOj2oQ+UF4OG8UvF1T17pawDPBARWnTJW5S286wHTy7yPgAGnTvwvNUJLznn6E/ 7hJ2 AIwKDsMKIvloeLCd7oj9U= 3, 27 -- Received: by 10.142.81.6 with SMTP id e6mr1940572wfb.205.1206058082290; 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Message-ID: {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} 3, 27 -- Subject: Double overhead charges 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From mdunlap-at-ucmerced.edu Fri Mar 21 10:13:26 2008 10, 24 -- Received: from mario.ucmerced.edu (mario.ucmerced.edu [169.236.10.223]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LFDPXQ012725 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:13:26 -0500 10, 24 -- Received: from engwin.ucmerced.edu ([169.236.142.218]) 10, 24 -- (authenticated bits=0) 10, 24 -- by mario.ucmerced.edu (8.12.10/8.12.9) with ESMTP id m2LFDNY0023221 10, 24 -- (version=TLSv1/SSLv3 cipher=EDH-RSA-DES-CBC3-SHA bits=168 verify=NOT) 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 07:13:23 -0800 (PST) 10, 24 -- From: "Mike Dunlap" {mdunlap-at-ucmerced.edu} 10, 24 -- Reply-To: "mdunlap-at-ucmerced.edu" {mdunlap-at-ucmerced.edu} 10, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 24 --
Question posed:
So if Prof. Marks wants to use the services at two universities, isn't this equivalent to him using twice the resources?
Answer (from a consultant's perspective):
Not exactly.
1. As a consultant (and one who does economic evaluations like capital equivalents and future worth comparisons), it is a matter of processing costs and the expenses associated with that (direct and indirect).
2. For Engineering and Service oriented firms like ours:
The standard Overhead (OH) charge on an item is 10-15%. This has to cover administration, accounting, taxes, and insurance.
Admin -
time to administer resources and file them with accounting, including proportion allocated to office space, computers, etc.
Accounting -
Paying for accountants to measure, input data, allocate, and pay
Taxes
Any addition taxes, either direct or indirect through increased effective rates to a company (this can occur through the purchase of capital investments that cannot be written off in the 1st year).
Insurance -
Most Errors & Emissions (E&O) liability insurance is a flat rate on gross. So if I pass through $100, then 0.5-4% (depending on field and rating) will be paid to my insurance company. So if I don't mark up the OH cost for this, I'll loose 0.5-4% of that money.
Portions of some General liability insurance is also billed at a flat rate and thus another cost.
3. So, depending on the relative size of the project, one will need to cover a base amount (say $50-$500 depending on the level of inquiry and setup) plus a percentage (%) of the pass-through.
For a small job like this, $100 + 10% would be expected in the real world. Otherwise, it is not worth touching.
4. As I write this, consider these aspects when purchasing Accessories to Electron Microscopes (CTL, EDX, Software, etc.). There is a balance between having the accessories under one umbrella, versus separate. This can be a big deal when it comes to servicing an instrument.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
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-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Monday, March 24, 2008 1:19 PM To: ph2-at-sprynet.com
Scott - You are confused in your interpretation of how overhead works. Overhead is charged on all purchases from a vendor. The bill (both the direct costs of the item and the indirect costs of overhead) to the funding agency occurs when purchases are made, not when the grant is awarded up front. Faculty sometimes think in those terms but we all realize it doesn't work that way when we get to the end of a grant and/or want to rebudget money from one category to another.
The double overhead in this case is to another school. If it costs X amount for my school to process a grant and pay for heat and electricity in my lab, then it is appropriate for them to charge overhead. If I buy an item from a vendor, they also sometimes feel it necessary to bill me for the true cost of the item to themselves plus enough to pay their own accountants, mailroom staff, webdevelopers, and technical directors (that is their overhead and it is built into the cost). So if Prof. Marks wants to use the services at two universities, isn't this equivalent to him using twice the resources?
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Monday, March 24, 2008 11:51 AM To: Phillips, Thomas E.
I don't think this is correct. Now it has been some time since I was a graduate student and since I was on a university faculty, but we never paid an overhead charge on a purchase from a vendor. When the grant was awarded, there was an overhead charge on that that was pretty hefty and that percentage varies from university to university. To me, what is unethical for Prof. Marks' university is to charge overhead charges on grant monies that has already been charged the university's overhead charges. That is nothing more than double taxation.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mdunlap-at-ucmerced.edu [mailto:mdunlap-at-ucmerced.edu] Sent: Friday, March 21, 2008 8:19 AM To: Walck-at-SouthBayTech.com
I am assuming that the funds to pay for the TEM time are coming from a grant. You are purchasing a service from another vendor (the other university) and your university will charge overhead on all non-equipment purchases.
Your service provider just happens to be a different university and this other university will charge overhead on all income coming in, just like your university. If this other university did not, you would be getting a cheaper rate than the in-house users of the TEM at the other university would. Remember the in-house users are paying $50/hr + overhead.
Mike
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One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 3, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.173]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0836R001151 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 19:08:03 -0500 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=BWxOVqx7df3bWjl8u0trWM8NZbKmo2/jVjRz+52j6CY=; 3, 27 -- b=LKgmS9NYkJGO6ybLDF1cLlKPhU6LIS2aFu8V9IJqA+FlUV/xACyupt0kMCG/Z/JhvFSxp5 nZsU fmeDg6jm7sMFdtK3fclLDk5l3LtQvmcMTwyjJ+kwBYYqS8Jtp2GCAH0PIRcq+9cJUenbPj7p wcP+ 9p9VPEsZIHviJYoVHwk84= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer -encoding:content-disposition; 3, 27 -- b=MG3PAKN5EscmHPVM9cdNoWZNbUySwy4cYXgYX0ZkufFz7V1CosVRXuQB3pHne+Q/Kg9vHC asvV LiSrviGejuOj2oQ+UF4OG8UvF1T17pawDPBARWnTJW5S286wHTy7yPgAGnTvwvNUJLznn6E/ 7hJ2 AIwKDsMKIvloeLCd7oj9U= 3, 27 -- Received: by 10.142.81.6 with SMTP id e6mr1940572wfb.205.1206058082290; 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Message-ID: {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} 3, 27 -- Subject: Double overhead charges 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From mdunlap-at-ucmerced.edu Fri Mar 21 10:13:26 2008 10, 24 -- Received: from mario.ucmerced.edu (mario.ucmerced.edu [169.236.10.223]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LFDPXQ012725 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:13:26 -0500 10, 24 -- Received: from engwin.ucmerced.edu ([169.236.142.218]) 10, 24 -- (authenticated bits=0) 10, 24 -- by mario.ucmerced.edu (8.12.10/8.12.9) with ESMTP id m2LFDNY0023221 10, 24 -- (version=TLSv1/SSLv3 cipher=EDH-RSA-DES-CBC3-SHA bits=168 verify=NOT) 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 07:13:23 -0800 (PST) 10, 24 -- From: "Mike Dunlap" {mdunlap-at-ucmerced.edu} 10, 24 -- Reply-To: "mdunlap-at-ucmerced.edu" {mdunlap-at-ucmerced.edu} 10, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 24 --
I am not sure where you do business but I pay a lot more than 10% OH for lots of things. Assuming you mean any markup in cost over true manufacturing cost. Overhead could be defined in different ways but it has a definite legal definition in a University environment. 10% would not begin to cover the subsidy my LM core runs nor would it cover the EM core on my campus. In fact, 50% wouldn't cover it. But I promise my administration to make money as soon as the library does. On the other hand, I don't expect my administrators to subsidize investigators at other universities.
You are correct that a University's indirect costs (what is being called "overhead" in this discussion) is an official negotiated rate between the federal govt and schools. If a federally funded user from another campus or company comes to us, we charge them the same rate as our internal users (+ overhead) since the law is that you can't charge different users different rates if they are paying with federal monies - my users are paying my standard hourly rate plus the routine indirect rate. So you are incorrect when you say it would not apply from one school to another - it does, and it's the law. We just had an audit by the feds and this point was stressed. If a user from another university was paying only the direct costs (i.e., hourly rate), they would be paying less than our own users (who pay the hourly rate + indirect costs). This is against the law. If a private corporation comes to us, we charge significantly more than federally funded grants since this is legal. Is our indirect rate fair? No, it is an underestimate of our expenses but it was the best we could negotiate with the feds.
I understand what you are saying in point 3 below but still don't see its relevance. We are talking about services not capital equipment which has no indirect costs (i.e., "overhead" in the sense of this discussion) in the way federal grants work.
In regards to point 4 below - as I said above, I run my core at a deficit and that doesn't reflect the original capital expenditures. Most of the instruments in my core were bought with University money (not federal). I have no budget for buying new or replacement instrumentation and don't include this cost in my hourly fees. I spent about 780K on my last Confocal including all the peripheral toys and software. At $30/hr, I would need to bill 26,000 hours. At 40 hrs a week, that would be 650 weeks to break even. Except I need to pay a service contract, staff salaries and benefits, etc. And this doesn't include the cost of the building, heat, electricity that come out of my "overhead". So I don't feel so bad asking an outside user to pay more than $30/hr since it is a bargain.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: Tony Havics [mailto:ph2-at-sprynet.com] Sent: Monday, March 24, 2008 2:20 PM To: Phillips, Thomas E.
Question posed:
So if Prof. Marks wants to use the services at two universities, isn't this equivalent to him using twice the resources?
Answer (from a consultant's perspective):
Not exactly.
1. As a consultant (and one who does economic evaluations like capital equivalents and future worth comparisons), it is a matter of processing costs and the expenses associated with that (direct and indirect).
2. For Engineering and Service oriented firms like ours:
The standard Overhead (OH) charge on an item is 10-15%. This has to cover administration, accounting, taxes, and insurance.
Admin -
time to administer resources and file them with accounting, including proportion allocated to office space, computers, etc.
Accounting -
Paying for accountants to measure, input data, allocate, and pay
Taxes
Any addition taxes, either direct or indirect through increased effective rates to a company (this can occur through the purchase of capital investments that cannot be written off in the 1st year).
Insurance -
Most Errors & Emissions (E&O) liability insurance is a flat rate on gross. So if I pass through $100, then 0.5-4% (depending on field and rating) will be paid to my insurance company. So if I don't mark up the OH cost for this, I'll loose 0.5-4% of that money.
Portions of some General liability insurance is also billed at a flat rate and thus another cost.
3. So, depending on the relative size of the project, one will need to cover a base amount (say $50-$500 depending on the level of inquiry and setup) plus a percentage (%) of the pass-through.
For a small job like this, $100 + 10% would be expected in the real world. Otherwise, it is not worth touching.
4. As I write this, consider these aspects when purchasing Accessories to Electron Microscopes (CTL, EDX, Software, etc.). There is a balance between having the accessories under one umbrella, versus separate. This can be a big deal when it comes to servicing an instrument.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
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-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Monday, March 24, 2008 1:19 PM To: ph2-at-sprynet.com
Scott - You are confused in your interpretation of how overhead works. Overhead is charged on all purchases from a vendor. The bill (both the direct costs of the item and the indirect costs of overhead) to the funding agency occurs when purchases are made, not when the grant is awarded up front. Faculty sometimes think in those terms but we all realize it doesn't work that way when we get to the end of a grant and/or want to rebudget money from one category to another.
The double overhead in this case is to another school. If it costs X amount for my school to process a grant and pay for heat and electricity in my lab, then it is appropriate for them to charge overhead. If I buy an item from a vendor, they also sometimes feel it necessary to bill me for the true cost of the item to themselves plus enough to pay their own accountants, mailroom staff, webdevelopers, and technical directors (that is their overhead and it is built into the cost). So if Prof. Marks wants to use the services at two universities, isn't this equivalent to him using twice the resources?
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Monday, March 24, 2008 11:51 AM To: Phillips, Thomas E.
I don't think this is correct. Now it has been some time since I was a graduate student and since I was on a university faculty, but we never paid an overhead charge on a purchase from a vendor. When the grant was awarded, there was an overhead charge on that that was pretty hefty and that percentage varies from university to university. To me, what is unethical for Prof. Marks' university is to charge overhead charges on grant monies that has already been charged the university's overhead charges. That is nothing more than double taxation.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mdunlap-at-ucmerced.edu [mailto:mdunlap-at-ucmerced.edu] Sent: Friday, March 21, 2008 8:19 AM To: Walck-at-SouthBayTech.com
I am assuming that the funds to pay for the TEM time are coming from a grant. You are purchasing a service from another vendor (the other university) and your university will charge overhead on all non-equipment purchases.
Your service provider just happens to be a different university and this other university will charge overhead on all income coming in, just like your university. If this other university did not, you would be getting a cheaper rate than the in-house users of the TEM at the other university would. Remember the in-house users are paying $50/hr + overhead.
Mike
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One of my students would like to use a TEM at another university since we don't have an appropriate instrument. The other university charges $50/hr and (for their own faculty) they have an overhead rate of 50% on research expenses. They are requesting that I pay $75/hr (i.e. they will add overhead) and then my university wants to tack on their own overhead rate (50%) as well.
I consider this unethical. I wonder if anyone has come across this before and has found a useful resolution beyond going bankrupt.
-- Professor Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 3, 27 -- From marksmsa-at-gmail.com Thu Mar 20 19:08:03 2008 3, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.173]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2L0836R001151 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 19:08:03 -0500 3, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so1423999wfg.21 3, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime -version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=BWxOVqx7df3bWjl8u0trWM8NZbKmo2/jVjRz+52j6CY=; 3, 27 -- b=LKgmS9NYkJGO6ybLDF1cLlKPhU6LIS2aFu8V9IJqA+FlUV/xACyupt0kMCG/Z/JhvFSxp5 nZsU fmeDg6jm7sMFdtK3fclLDk5l3LtQvmcMTwyjJ+kwBYYqS8Jtp2GCAH0PIRcq+9cJUenbPj7p wcP+ 9p9VPEsZIHviJYoVHwk84= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer -encoding:content-disposition; 3, 27 -- b=MG3PAKN5EscmHPVM9cdNoWZNbUySwy4cYXgYX0ZkufFz7V1CosVRXuQB3pHne+Q/Kg9vHC asvV LiSrviGejuOj2oQ+UF4OG8UvF1T17pawDPBARWnTJW5S286wHTy7yPgAGnTvwvNUJLznn6E/ 7hJ2 AIwKDsMKIvloeLCd7oj9U= 3, 27 -- Received: by 10.142.81.6 with SMTP id e6mr1940572wfb.205.1206058082290; 3, 27 -- Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Received: by 10.142.153.15 with HTTP; Thu, 20 Mar 2008 17:08:02 -0700 (PDT) 3, 27 -- Message-ID: {e13ba6260803201708l7b79b280x49b79a9b8e22e792-at-mail.gmail.com} 3, 27 -- Date: Thu, 20 Mar 2008 19:08:02 -0500 3, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 3, 27 -- To: microscopy {Microscopy-at-microscopy.com} 3, 27 -- Subject: Double overhead charges 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 10, 24 -- From mdunlap-at-ucmerced.edu Fri Mar 21 10:13:26 2008 10, 24 -- Received: from mario.ucmerced.edu (mario.ucmerced.edu [169.236.10.223]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2LFDPXQ012725 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 10:13:26 -0500 10, 24 -- Received: from engwin.ucmerced.edu ([169.236.142.218]) 10, 24 -- (authenticated bits=0) 10, 24 -- by mario.ucmerced.edu (8.12.10/8.12.9) with ESMTP id m2LFDNY0023221 10, 24 -- (version=TLSv1/SSLv3 cipher=EDH-RSA-DES-CBC3-SHA bits=168 verify=NOT) 10, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Mar 2008 07:13:23 -0800 (PST) 10, 24 -- From: "Mike Dunlap" {mdunlap-at-ucmerced.edu} 10, 24 -- Reply-To: "mdunlap-at-ucmerced.edu" {mdunlap-at-ucmerced.edu} 10, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 24 --
Folks
The "Indirect Cost" rate (aka overhead) is not anything that anyone PI on a campus is going to change. That calcuation and negotiation happens at a much higher level. For my location, that happens in Albany (hopefully not in any motels).
However, the local rate structure for equipment usage is determined locally on my campus with the folks from our "research accounting department". This rate (recharge) is determined by the cost to operate/use/maintain the equipment. For this rate, I have some input.
For "off-campus" users (every those with Federal grants), we bill out the recharge rate plus 15%. The 15% does not come back to the lab. It goes to the accountants, grant managers, paper-pushers, etc.....
Nobody sees a double tax of the full "indirect cost" rate from BOTH universities.
just my two cents,
JQ
PS: OoO away.............
==============================Original Headers============================== 10, 12 -- From jquinn-at-www.matscieng.sunysb.edu Mon Mar 24 18:15:55 2008 10, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2ONFnth008800 10, 12 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2008 18:15:51 -0500 10, 12 -- Received: (from jquinn-at-localhost) 10, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m2P0G7J18516 10, 12 -- for microscopy-at-microscopy.com; Mon, 24 Mar 2008 19:16:07 -0500 10, 12 -- Date: Mon, 24 Mar 2008 19:16:07 -0500 10, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 10, 12 -- Message-Id: {200803250016.m2P0G7J18516-at-www.matscieng.sunysb.edu} 10, 12 -- To: microscopy-at-microscopy.com 10, 12 -- Subject: re: Double charge (headache) ==============================End of - Headers==============================
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Email: ramshes-at-musc.edu Name: Venkat Ramshesh
Organization: MUSC
Title-Subject: [Filtered] Deadline for applications extended to April 15 for LMB workshop
Question: Second Charleston Workshop on
LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Medical University of South Carolina
May 18-23, 2008
The Second Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB) Workshop provides a solid introduction to the concepts and practical applications of light microscopy relevant to modern cell and molecular biology. Students will have opportunities for extensive hands-on experience with state-of-the-art equipment for optical imaging, digital image processing, and fluorescence and confocal/multiphoton microscopy guided by experienced academic and commercial faculty. Lectures and laboratory exercises will include: optics of image formation; microscope alignment; phase contrast and differential interference contrast microscopy; video and digital cameras; contrast enhancement by analog and digital image processing; principles of fluorescence and fluorescence microscopy; ion imaging and fluorescent probes, including green fluorescent protein; fluorescence resonance energy transfer; and laser scanning confocal and multiphoton microscopy. A commercial faculty representing leading microscope manufacturers will make available for student use the latest and most advanced instrumentation for light microscopy, image detection and computerized image analysis. The LMB Workshop is designed for doctoral level scientists, advanced pre-doctoral students and high level technical personnel. No prior experience with microscopy is required. All students will benefit from in-depth interaction with instructors. Students are encouraged to bring their own specimens for analysis.
Tuition: $750.00
Application Deadline: April 15, 2008
Principal Instructors:
John J. Lemasters, M.D., Ph.D., Organizer
P. Darwin Bell, Ph.D.
Margaret Kelly, Ph.D.
Peter Komlosi, Ph.D.
Anna-Liisa Nieminen, Ph.D.
Venkat Ramshesh, Ph.D.
To apply, send a curriculum vita and a brief letter describing your research interests and reasons for enrolling. Because the course is expected to be oversubscribed, applicants should inquire as soon as possible. Please indicate your complete mailing address, telephone/fax number and email address. Full consideration will be given to applications received by April 15, 2008.
For further information or to apply, contact:
Venkat K Ramshesh Medical University of South Carolina Center for Cell Death, Injury and Regeneration and Hollings Cancer Center 280 Calhoun Street, PO Box 250140 Charleston, SC 29425 Telephone (843) 792- 3530, FAX (843) 792-1617 E-mail: ramshes-at-musc.edu
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Email: andel-at-cperi.certh.gr Name: Andreas Delimitis
Organization: Centre for Research & Technology Hellas - CPERI
Title-Subject: [Filtered] Oil leak in rotary pump of JEOL 2011 TEM
Question: Dear Listers,
I was wondering if anyone has experience with oil leaking from a TEM oil rotary pump.
We have a small, but continous, oil leak from the rotary pump of our JEOL 2011 TEM. The rotary pump model is RP-100GV(S), from Maruyama Vacuum Pumps Co. and the leak is from a small opening, in the middle of a circular window, at the rear of the pump body. Opening the window did not reveal any apparent connection between this and the oil tank to justify the leak.
Replacing the old oil with a new one did not have any beneficial effect. Also, both the oil level gauge and the drain plug do not leak at all.
Thanks in advance, Andreas
--------------------------------------------- Dr. Andreas Delimitis Associate (Application) Scientist C' Chemical Process Engineering Research Institute (CPERI) Centre for Research & Technology - Hellas (CERTH) 6th Km. Charilaou - Thermi road 57001 Thermi, Thessaloniki GREECE Tel: +30 2310 498259 E-mail: andel-at-cperi.certh.gr ---------------------------------------------
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] airlock/drybox for air sensitvie TEM samples
Question: There is a possibility, which is now just at the internal proposal stage, of a project that would we require that I handle air-senitive TEM specimens and load them into my top entry Hitachi H9000. I would appreciate any suggestions as to how to go about this, as well as an assessment of the price of the dry box or whatever else goes into this sort of thing.
These samples would also contain Li, which might also have some deliterious effect on the TEM. I would also appreciate comments addressing this issue.
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Email: hooghan-at-verizon.net Name: Bobby Hooghan
Organization: Ovonyx Technologies Inc.
Title-Subject: [Filtered] Accuracy of thin film EDS measurements!
Question: All, We would like to tap on the resources of this list server to answer a few of our questions.
We are a small company making phase change memories. We sputter deposit chalcogenide materials with the thickness of the films ranging from 5-75 nm. We have an FEI NNL dual beam system with an EDS, and a WDS systems on board. We use this tool to check for thickness and composition (using EDS) of the films we deposit. We cleave the wafer and look at it edge on for accurate thickness measurements.
Our questions are as follows:
1. Using about 5-20 kev and about 1nm spot size, how accurate can we get the composition of the films that we deposit, would 10% be reasonable and attainable? That is our challenge currently. We can get a reasonable cps and dead time in our EDS system. 2. Any other method of getting accurate composition measurements on these films?
Thanks in advance and apologize if it comes as a repeat posting, Bobby Hooghan Ovonyx Technologies Inc. Rochester Hills MI 248-299-6014
Hi Bobby, The bottom line is that if one doesn't correct for the thin film geometry one will have unpredictably poor accuracy whenever substrate x-rays are observed.
If you are interested in details, please contact me off-line and I can send you some PPT slides that demonstrate this with a NIST thin film standard I measured. john
At 08:21 PM 3/24/2008, hooghan-at-verizon.net wrote:
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==============================Original Headers============================== 8, 26 -- From donovan-at-uoregon.edu Mon Mar 24 22:57:03 2008 8, 26 -- Received: from QMTA05.westchester.pa.mail.comcast.net (qmta05.westchester.pa.mail.comcast.net [76.96.62.48]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2P3v2rq023928 8, 26 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2008 22:57:02 -0500 8, 26 -- Message-Id: {200803250357.m2P3v2rq023928-at-ns.microscopy.com} 8, 26 -- Received: from OMTA03.westchester.pa.mail.comcast.net ([76.96.62.27]) 8, 26 -- by QMTA05.westchester.pa.mail.comcast.net with comcast 8, 26 -- id 5ACr1Z00T0bG4ec550CP00; Tue, 25 Mar 2008 03:55:44 +0000 8, 26 -- Received: from SOURCE.uoregon.edu ([71.193.189.123]) 8, 26 -- by OMTA03.westchester.pa.mail.comcast.net with comcast 8, 26 -- id 5Fwz1Z00D2gBFvr3P00000; Tue, 25 Mar 2008 03:57:00 +0000 8, 26 -- X-Authority-Analysis: v=1.0 c=1 a=uFJ_ivy8914A:10 a=Zx37jsudAAAA:8 8, 26 -- a=dlBkIlLSAAAA:8 a=ZyhTh9hsfYjXWLvi9MYA:9 a=h4dkY8lI39DxchEKGicA:7 8, 26 -- a=l075dyfRzDzZy6Lq_ztqo6ZRFgIA:4 a=ILCZio5HsAgA:10 a=G91thX30KR8A:10 8, 26 -- a=WFqUXA311JwA:10 a=50e4U0PicR4A:10 8, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 26 -- Date: Mon, 24 Mar 2008 20:57:02 -0700 8, 26 -- To: hooghan-at-verizon.net 8, 26 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 8, 26 -- Subject: Re: [Microscopy] viaWWW: Accuracy of thin film EDS 8, 26 -- measurements! 8, 26 -- Cc: microscopy-at-microscopy.com 8, 26 -- In-Reply-To: {200803250321.m2P3LEaf001214-at-ns.microscopy.com} 8, 26 -- References: {200803250321.m2P3LEaf001214-at-ns.microscopy.com} 8, 26 -- Mime-Version: 1.0 8, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Our Leica UC6 will not turn on, and I wonder if a fuse has blown - but where would this be? I've inspected the power adapter, the control module (older touch-screen model) and microtome itself. Any ideas, anyone?
thanks, Rosemary White
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
==============================Original Headers============================== 6, 21 -- From prvs=Rosemary.White=96352fb0b-at-csiro.au Tue Mar 25 00:16:39 2008 6, 21 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2P5GcVw009581 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 00:16:39 -0500 6, 21 -- X-IronPort-AV: E=Sophos;i="4.25,550,1199624400"; 6, 21 -- d="scan'208";a="189019521" 6, 21 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 6, 21 -- by act-ironport-int.csiro.au with ESMTP; 25 Mar 2008 16:16:38 +1100 6, 21 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 6, 21 -- Tue, 25 Mar 2008 16:16:36 +1100 6, 21 -- User-Agent: Microsoft-Entourage/11.0.0.040405 6, 21 -- Date: Tue, 25 Mar 2008 16:21:48 +1100 6, 21 -- Subject: UC6 fuse? 6, 21 -- From: Rosemary White {rosemary.white-at-csiro.au} 6, 21 -- To: {Microscopy-at-microscopy.com} 6, 21 -- Message-ID: {C40ED71C.A0D9%rosemary.white-at-csiro.au} 6, 21 -- Mime-version: 1.0 6, 21 -- Content-type: text/plain; 6, 21 -- charset="US-ASCII" 6, 21 -- Content-transfer-encoding: 7bit 6, 21 -- X-OriginalArrivalTime: 25 Mar 2008 05:16:37.0226 (UTC) FILETIME=[666464A0:01C88E37] ==============================End of - Headers==============================
This depends on what the samples are. "Biological" doesn't help. Bone is biological, cultured cells are biological, medical implants, pollen, fungal hyphae ... etc. etc. etc. It also depends on how the samples were processed: fixed, dehydrated, mounted, stored. It also depends on the operating parameters of the SEM, kV and so forth. A good starting point is "Artifacts in Biological Electron Microscopy" by R. F. E. Crang and K.L. Klomparens (which I really wish they'd update with a new edition). And microscopy journals.
If you have questions about specific samples treated in specific ways, we could give you much more helpful answers.
Phil
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==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Tue Mar 25 07:15:31 2008 4, 24 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2PCFUOa011525 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 07:15:30 -0500 4, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 24 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m2PCTi9P001026; 4, 24 -- Tue, 25 Mar 2008 08:29:45 -0400 4, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 24 -- Tue, 25 Mar 2008 08:15:31 -0400 4, 24 -- Mime-Version: 1.0 4, 24 -- Message-Id: {f06240801c40e9bfdc27c-at-[141.209.160.249]} 4, 24 -- In-Reply-To: {200803250318.m2P3IDuw025524-at-ns.microscopy.com} 4, 24 -- References: {200803250318.m2P3IDuw025524-at-ns.microscopy.com} 4, 24 -- Date: Tue, 25 Mar 2008 08:15:26 -0400 4, 24 -- To: ppotts-at-heart.thi.tmc.edu 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: Re: [Microscopy] viaWWW: Artifacts in Critical Point Drying and 4, 24 -- Gold Sputtering 4, 24 -- Cc: Microscopy-at-microscopy.com 4, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 24 -- X-OriginalArrivalTime: 25 Mar 2008 12:15:31.0122 (UTC) FILETIME=[EB5C9120:01C88E71] 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Spam-Score: -4 () L_EXCH_MF 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Is the oil leaking from that small window on the side of the pump? Not so long time ago I had almost the same issue, and I had to disassemble the pump completely, the simmering at the shaft of the pump was oldened.
Laci
==============================Original Headers============================== 3, 31 -- From jakabfarkaslaszlo-at-gmail.com Tue Mar 25 07:36:13 2008 3, 31 -- Received: from wr-out-0506.google.com (wr-out-0506.google.com [64.233.184.238]) 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2PCaCkv024599 3, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 07:36:12 -0500 3, 31 -- Received: by wr-out-0506.google.com with SMTP id c57so2189775wra.9 3, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 05:36:12 -0700 (PDT) 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 31 -- d=gmail.com; s=beta; 3, 31 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references:x-google-sender-auth; 3, 31 -- bh=NFZ2WfZcAd5gCx05O+toqynjJpXaAxZ/HJuGsBym+yc=; 3, 31 -- b=Qs+kOJmFy524tB0J7NpCcDilcv7duL7CczdmOXCYVnegoIN5vKQJCvr0LjUYdXWmZq+mJiyugR4A0tPrCLhfSRcPc3hKKvlsoEAVoJVYX4zgPhIFJeCpy9Do5pae2bmGRK5JBULtWU2qjfuCuqZv4W6OsnxaG8LUCMvx2Nods4E= 3, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 31 -- d=gmail.com; s=beta; 3, 31 -- h=message-id:date:from:sender:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references:x-google-sender-auth; 3, 31 -- b=eft80b4d9LrLBamPy2bE0HfttXXECm6nNLWgLqaVJ0pxxONX2BkCrNhQRZIZjitxb+JIlxG/19yLK1TcYPbshqXMwGkX3/ffZjxbvnhjpDnMGVZ8CAnM/A7VPOE6sSS2jHohcUi/PUBjzsZz15/ijdj7giTMArAMQFHCVii4hxo= 3, 31 -- Received: by 10.141.163.12 with SMTP id q12mr3213406rvo.190.1206448571884; 3, 31 -- Tue, 25 Mar 2008 05:36:11 -0700 (PDT) 3, 31 -- Received: by 10.140.202.5 with HTTP; Tue, 25 Mar 2008 05:36:10 -0700 (PDT) 3, 31 -- Message-ID: {a3da032b0803250536q602bf65ct148504e96358360b-at-mail.gmail.com} 3, 31 -- Date: Tue, 25 Mar 2008 14:36:10 +0200 3, 31 -- From: "=?ISO-8859-1?Q?Laszl=F3_Jakab-Farkas?=" {jflaci-at-ms.sapientia.ro} 3, 31 -- Sender: jakabfarkaslaszlo-at-gmail.com 3, 31 -- To: Microscopy-at-microscopy.com 3, 31 -- Subject: Re: [Microscopy] viaWWW: Oil leak in rotary pump of JEOL 2011 TEM 3, 31 -- In-Reply-To: {200803250324.m2P3ObKh010072-at-ns.microscopy.com} 3, 31 -- MIME-Version: 1.0 3, 31 -- Content-Type: text/plain; charset=ISO-8859-1 3, 31 -- Content-Transfer-Encoding: 7bit 3, 31 -- Content-Disposition: inline 3, 31 -- References: {200803250324.m2P3ObKh010072-at-ns.microscopy.com} 3, 31 -- X-Google-Sender-Auth: 3cee43aa65c33984 ==============================End of - Headers==============================
Actually the external user does see double charge if they are paying with federal money. Their grant would already have been docked for their institution's indirect costs (with amount determined through negotiations between their institution and the granting agencies). They then pay the overhead assigned by the institution who did the work for them. Even private companies will have overhead to cover. It just is taken out up front.
I do not see any way around this. Every institution, public or private and for or not-for profit has overhead costs for supplying the physical facilities and institution management. Anyone using the facilities, whether internal or external, needs to share in these costs. That is just the way it is.
Unfortunately we also do not get back these extra fees generated by external use at the present time. This policy is under discussion at the moment so hopefully it will change in the future.
However, it is a lot less expensive to go out and use a needed piece of equipment elsewhere on occasion, paying user fees plus overhead than to pay for it's original cost plus maintenance. If there is significant need on your campus than start writing grants to acquire the instrumentation and hopefully user fees will cover future operating and maintenance costs.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
} From: {jquinn-at-www.matscieng.sunysb.edu} } Reply-To: {jquinn-at-www.matscieng.sunysb.edu} } Date: Mon, 24 Mar 2008 18:19:25 -0500 } To: {dsherman-at-purdue.edu} } Subject: [Microscopy] re: Double charge (headache) } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Folks } } The "Indirect Cost" rate (aka overhead) is not anything that } anyone PI on a campus is going to change. That calcuation } and negotiation happens at a much higher level. For my location, } that happens in Albany (hopefully not in any motels). } } However, the local rate structure for equipment usage is } determined locally on my campus with the folks from } our "research accounting department". This rate (recharge) } is determined by the cost to operate/use/maintain the equipment. } For this rate, I have some input. } } For "off-campus" users (every those with Federal grants), } we bill out the recharge rate plus 15%. The 15% does } not come back to the lab. It goes to the accountants, } grant managers, paper-pushers, etc..... } } Nobody sees a double tax of the full "indirect cost" rate } from BOTH universities. } } just my two cents, } } JQ } } PS: OoO away............. } } } ==============================Original Headers============================== } 10, 12 -- From jquinn-at-www.matscieng.sunysb.edu Mon Mar 24 18:15:55 2008 } 10, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu } [129.49.36.33]) } 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m2ONFnth008800 } 10, 12 -- for {microscopy-at-microscopy.com} ; Mon, 24 Mar 2008 18:15:51 -0500 } 10, 12 -- Received: (from jquinn-at-localhost) } 10, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m2P0G7J18516 } 10, 12 -- for microscopy-at-microscopy.com; Mon, 24 Mar 2008 19:16:07 -0500 } 10, 12 -- Date: Mon, 24 Mar 2008 19:16:07 -0500 } 10, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} } 10, 12 -- Message-Id: {200803250016.m2P0G7J18516-at-www.matscieng.sunysb.edu} } 10, 12 -- To: microscopy-at-microscopy.com } 10, 12 -- Subject: re: Double charge (headache) } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 23 -- From dsherman-at-purdue.edu Tue Mar 25 10:58:28 2008 9, 23 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2PFwREu013416 9, 23 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 10:58:28 -0500 9, 23 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 23 -- Tue, 25 Mar 2008 11:58:24 -0400 9, 23 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 9, 23 -- Tue, 25 Mar 2008 15:58:24 +0000 9, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 23 -- Date: Tue, 25 Mar 2008 11:58:23 -0400 9, 23 -- Subject: Re: [Microscopy] re: Double charge (headache) 9, 23 -- From: Debby Sherman {dsherman-at-purdue.edu} 9, 23 -- To: {jquinn-at-www.matscieng.sunysb.edu} , 9, 23 -- "message to: MSA list" {microscopy-at-microscopy.com} 9, 23 -- Message-ID: {C40E995F.2A6E1%dsherman-at-purdue.edu} 9, 23 -- Thread-Topic: [Microscopy] re: Double charge (headache) 9, 23 -- Thread-Index: AciOkQ2fTGX71fqEEdyqnAARJN08Mg== 9, 23 -- In-Reply-To: {200803242319.m2ONJPcg012250-at-ns.microscopy.com} 9, 23 -- Mime-version: 1.0 9, 23 -- Content-type: text/plain; 9, 23 -- charset="US-ASCII" 9, 23 -- Content-transfer-encoding: 7bit 9, 23 -- X-OriginalArrivalTime: 25 Mar 2008 15:58:24.0195 (UTC) FILETIME=[0E559130:01C88E91] ==============================End of - Headers==============================
Mark, I've given this some thought recently because as you know we sell the SampleSaver™ storage container and the Plasma Trimmer™ and the Plasma Trimming™ Rod. The SampleSaver™ is designed for storing atmospheric sensitive samples. The Plasma Trimming™ technique removes the damage and any oxide layers from the surface of TEM samples. So the following are some of the thoughts that I've had to answer exactly the same question that you have asked here.
We have racks that are specifically made for TEM samples and FIB-prepared TEM samples to fit into the SamplSaver™ containers. But these units are for storage and transportation purposes. If you have a glove box such as the one made by Omniprobe for loading and inserting air sensitive samples, then you can open and close our storage containers within their glovebox without the need to purge our containers and then use their system to transfer between FIB and TEM systems. If you don't have an FIB, you could still use their system. Their solution may be what you want. Here is the URL for their product: http://www.omniprobe.com/products/sst.htm I thought that our product was a perfect companion product for their Sensitive Sample Transfer system.
Another option that you may consider is to use a glove bag. This can also be used with our SampleSaver™ storage containers for loading and unloading samples from a TEM rod. There are two types of these, one large hole and two large hole models. These holes can be strapped around an object and then opened, maintaining your inert atmosphere. My idea has been to use these to bring our SampleSaver™ containers into them, load or unload the TEM rod, and then bring the bag with the TEM rod to the microscope and tie it around the entry port of the microscope and then to start a N2 purge. We also have the need for using something like this from taking our samples from a plasma cleaner after Plasma Trimming™ without the sample re-oxidizing. After Plasma Trimming™ the oxide is removed from the sample. I think that the glove bag solution would again work for this purpose. You would tie one of the large diameter ports to either the adapter port on the plasma cleaner or in our PC2000 case, around the chamber lid. Once the sample is in the bag, then it could be loaded in a SampleSaver™ for later insertion into a TEM rod, or it could be directly transferred to the TEM rod. Extending this idea further, samples that are removed from ion mills could be prevented from oxidizing using this approach.
We had seriously looked at marketing glove bags with our SampleSaver™ for exactly the purpose you are asking the question for. We decided not to do that for a number of reasons but instead have decided to just recommend the source. The bags were made by I2R whose assets were purchased by Glas-Col. See the following URL: http://www.glascol.com/data/file/webcontent/file-document-46.pdf
In addition, there are some other products that may be suitable for your purpose: http://www.sigmaaldrich.com/catalog/search/TablePage/9582543 http://www.coleparmer.com/catalog/product_view.asp?sku=0440838 http://www.erlab-dfs.com/us/index.html
I hope this information is useful to you.
Disclaimer: SBT manufactures and sells the SampleSaver(TM) storage container, PC-2000 plasma cleaner, Plasma Trimmer™, and Plasma Trimming™ rod. We do not have any commercial interest in either Omniprobe or Glas-Col.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: twigg-at-estd.nrl.navy.mil [mailto:twigg-at-estd.nrl.navy.mil] Sent: Monday, March 24, 2008 8:15 PM To: Walck-at-SouthBayTech.com
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] airlock/drybox for air sensitvie TEM samples
Question: There is a possibility, which is now just at the internal proposal stage, of a project that would we require that I handle air-senitive TEM specimens and load them into my top entry Hitachi H9000. I would appreciate any suggestions as to how to go about this, as well as an assessment of the price of the dry box or whatever else goes into this sort of thing.
These samples would also contain Li, which might also have some deliterious effect on the TEM. I would also appreciate comments addressing this issue.
Hi all, we are still searching for a suitable and surplus to your requirements, used but functioning TEM that may become available some time this year, if you have any suggestions could you let me know please, David.
==============================Original Headers============================== 2, 25 -- From david.llewellyn-at-anu.edu.au Tue Mar 25 16:43:47 2008 2, 25 -- Received: from anumail6.anu.edu.au (anumail6.anu.edu.au [130.56.64.140]) 2, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2PLhiWY017214 2, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 16:43:45 -0500 2, 25 -- Received: from smtphost.anu.edu.au (ds2.anu.edu.au [130.56.64.54]) 2, 25 -- by anumail6.anu.edu.au (8.13.8/8.13.8) with ESMTP id m2PLhglj004360 2, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 08:43:42 +1100 (EST) 2, 25 -- (envelope-from david.llewellyn-at-anu.edu.au) 2, 25 -- Received: from rsphymail.anu.edu.au (rsphymail.anu.edu.au [150.203.176.80]) 2, 25 -- by smtphost.anu.edu.au (8.14.1/8.14.1) with ESMTP id m2PLhgCc008590 2, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 2, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 08:43:42 +1100 (EST) 2, 25 -- Received: from [150.203.181.28] ([150.203.181.28]) 2, 25 -- by rsphymail.anu.edu.au (8.13.8/8.13.8) with ESMTP id m2PLhgL6010704 2, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 08:43:42 +1100 2, 25 -- Message-ID: {47E9720D.3090100-at-anu.edu.au} 2, 25 -- Date: Wed, 26 Mar 2008 08:43:41 +1100 2, 25 -- From: David Llewellyn {david.llewellyn-at-anu.edu.au} 2, 25 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 2, 25 -- MIME-Version: 1.0 2, 25 -- To: Microscopy-at-microscopy.com 2, 25 -- Subject: TEM 2, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 25 -- Content-Transfer-Encoding: 7bit 2, 25 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.25.142729 internal ==============================End of - Headers==============================
I am doing TEM on several cell cultures. The investigator is sure that some cell lines "appear" to have many vacuoles and other cell lines "appear" to have few vacuoles or none. The investigator would like me to quantify this condition. My question is how does one quantify this in such a way as to be statistically meaningful?
Secondly, the investigator feels that some cell lines have more mitochondria, while other cell lines have fewer mitochondria. Again they would like to get quantitative data of this condition. What are people's thoughts on how to approach this question?
If any respondent is really curious as to what I have already tried to explain to the investigator please include your telephone number and I will gladly call you. All help is appreciated.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 7, 20 -- From tbargar-at-unmc.edu Tue Mar 25 18:36:48 2008 7, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2PNamLN001969 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 18:36:48 -0500 7, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 7, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id F3ED3A0060 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 18:36:47 -0500 (CDT) 7, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 7, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id B2F56398060 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 18:36:46 -0500 (CDT) 7, 20 -- Subject: Vacuolated cells vs. non vacuolated cells 7, 20 -- To: Microscopy-at-MSA.Microscopy.com 7, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 7, 20 -- Message-ID: {OF76288709.261A67AE-ON86257417.007F5CB5-86257417.0081B55E-at-unmc.edu} 7, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 7, 20 -- Date: Tue, 25 Mar 2008 18:36:45 -0500 7, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 03/25/2008 06:36:46 7, 20 -- PM 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
I just got my new copy of Microscopy Today in the mail, and have proceeded my usual course of devouring it, when I cam across an advertisement for a new tabletop SEM from JEOL. Are tabletop SEMs the new bandwagon product for manufacturers to be producing now? I also saw an email regarding an Evex tabletop analytical model.
Also in this issue is a paper on a smaller TEM made possible by improved cooling methods.
With all of this miniaturization, I'm just curious what everyone thinks about the possibility of having SEMs and TEMs small enough to take into the field (Eventually)?
In general, do you see the push for smaller instruments as a positive move forward, or a quick "Bells and whistles" attraction to earn new revenue?
Just curious,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 7, 27 -- From kraftpiano-at-gmail.com Tue Mar 25 22:34:02 2008 7, 27 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.227]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2Q3Y2P2023202 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 22:34:02 -0500 7, 27 -- Received: by wx-out-0506.google.com with SMTP id h30so4872047wxd.21 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 20:34:01 -0700 (PDT) 7, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 27 -- d=gmail.com; s=beta; 7, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 7, 27 -- bh=vOrNr8SN6aULxK3i4pvVLM0gpObvTC1G/VUI1AKmJ28=; 7, 27 -- b=uCirNn3/Yv2PzDv3ZMQK9YiA585EDUwens0WElfmd59zltcLagSD5fBBwGtZRrDdIspUWmeXfFUvDjtQ2xbJJPlqXepm3enFY9phvDAFfenFkbj1Ud45SKqwCuInM78ilszicH1jwhjRMU7xCm7c19IezmEPON5Qh9dh17GW+PE= 7, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 27 -- d=gmail.com; s=beta; 7, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 7, 27 -- b=UwQtwLMN19o1pAajIkqc5SUTkenwRPpe0j9bJMawUlW2uK43wiT+yDfq9x8IzpZ3b5fB1/Oexncu35juy+GrePwpHZnDMThkwrpRFLrwnw8CBmeRorKLytIfkBqEk+OuJG3IlvUjbyqddIgT/s2POpd4p8oYfIW+Qe5jHOCOSZA= 7, 27 -- Received: by 10.140.133.10 with SMTP id g10mr4057463rvd.170.1206502440870; 7, 27 -- Tue, 25 Mar 2008 20:34:00 -0700 (PDT) 7, 27 -- Received: by 10.140.161.11 with HTTP; Tue, 25 Mar 2008 20:34:00 -0700 (PDT) 7, 27 -- Message-ID: {25e2b0d20803252034k3e389d71hb24de980093ee645-at-mail.gmail.com} 7, 27 -- Date: Tue, 25 Mar 2008 23:34:00 -0400 7, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 7, 27 -- To: microscopy-at-microscopy.com 7, 27 -- Subject: Table top SEM trend? 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1 7, 27 -- Content-Transfer-Encoding: 7bit 7, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ben.vandenberg-at-valeinco.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ben.vandenberg-at-valeinco.com Name: Ben Vanden Berg
Title-Subject: [Filtered] LM Equipment, Zeiss, Standard Universal and Ultraphot II
Question: I have 3 microscopes which have been collecting dust for years and I would like to sell or donate
Does anyone know the value of the following items and the best place to auction equipment like this?
All microscopes are compound models, Zeiss, 1960ís vintage
1 standard universal, polarizing, transmitted light equipment for reflected light 1 standard universal, polarizing, transmitted light, florescence and photography equipped 1 ultraphot II, monster photography machine numerous accessories
Well I guy I met through a mutual friend that developed an SEM for use on Mars. The entire column is 3 inches tall. I tried to convince him to commercialize it but I guess the NASA funding dried up and he has put it aside.
Tom Kaye
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Tuesday, March 25, 2008 8:39 PM To: tom-at-tomkaye.com
I just got my new copy of Microscopy Today in the mail, and have proceeded my usual course of devouring it, when I cam across an advertisement for a new tabletop SEM from JEOL. Are tabletop SEMs the new bandwagon product for manufacturers to be producing now? I also saw an email regarding an Evex tabletop analytical model.
Also in this issue is a paper on a smaller TEM made possible by improved cooling methods.
With all of this miniaturization, I'm just curious what everyone thinks about the possibility of having SEMs and TEMs small enough to take into the field (Eventually)?
In general, do you see the push for smaller instruments as a positive move forward, or a quick "Bells and whistles" attraction to earn new revenue?
Just curious,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 7, 27 -- From kraftpiano-at-gmail.com Tue Mar 25 22:34:02 2008 7, 27 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.227]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2Q3Y2P2023202 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 22:34:02 -0500 7, 27 -- Received: by wx-out-0506.google.com with SMTP id h30so4872047wxd.21 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 20:34:01 -0700 (PDT) 7, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 27 -- d=gmail.com; s=beta; 7, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 7, 27 -- bh=vOrNr8SN6aULxK3i4pvVLM0gpObvTC1G/VUI1AKmJ28=; 7, 27 -- b=uCirNn3/Yv2PzDv3ZMQK9YiA585EDUwens0WElfmd59zltcLagSD5fBBwGtZRrDdIspUWmeXfF UvDjtQ2xbJJPlqXepm3enFY9phvDAFfenFkbj1Ud45SKqwCuInM78ilszicH1jwhjRMU7xCm7c19 IezmEPON5Qh9dh17GW+PE= 7, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 27 -- d=gmail.com; s=beta; 7, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer -encoding:content-disposition; 7, 27 -- b=UwQtwLMN19o1pAajIkqc5SUTkenwRPpe0j9bJMawUlW2uK43wiT+yDfq9x8IzpZ3b5fB1/Oexn cu35juy+GrePwpHZnDMThkwrpRFLrwnw8CBmeRorKLytIfkBqEk+OuJG3IlvUjbyqddIgT/s2POp d4p8oYfIW+Qe5jHOCOSZA= 7, 27 -- Received: by 10.140.133.10 with SMTP id g10mr4057463rvd.170.1206502440870; 7, 27 -- Tue, 25 Mar 2008 20:34:00 -0700 (PDT) 7, 27 -- Received: by 10.140.161.11 with HTTP; Tue, 25 Mar 2008 20:34:00 -0700 (PDT) 7, 27 -- Message-ID: {25e2b0d20803252034k3e389d71hb24de980093ee645-at-mail.gmail.com} 7, 27 -- Date: Tue, 25 Mar 2008 23:34:00 -0400 7, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 7, 27 -- To: microscopy-at-microscopy.com 7, 27 -- Subject: Table top SEM trend? 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; charset=ISO-8859-1 7, 27 -- Content-Transfer-Encoding: 7bit 7, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 16, 22 -- From tom-at-TomKaye.com Tue Mar 25 23:13:36 2008 16, 22 -- Received: from w2k3-exchfe.mmsasp.local (mx1.mmsasp.com [66.102.127.13]) 16, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2Q4DaMr016533 16, 22 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 23:13:36 -0500 16, 22 -- Received: from tk7c797a275194 ([67.134.108.9]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959); 16, 22 -- Tue, 25 Mar 2008 23:13:35 -0500 16, 22 -- From: "Tom Kaye" {tom-at-TomKaye.com} 16, 22 -- To: {microscopy-at-microscopy.com} 16, 22 -- Subject: RE: [Microscopy] Table top SEM trend? 16, 22 -- Date: Tue, 25 Mar 2008 21:13:34 -0700 16, 22 -- Message-ID: {GOEAIJOJDGCJBPIFHOBGGEIFPIAA.tom-at-tomkaye.com} 16, 22 -- MIME-Version: 1.0 16, 22 -- Content-Type: text/plain; 16, 22 -- charset="iso-8859-1" 16, 22 -- Content-Transfer-Encoding: 7bit 16, 22 -- X-Priority: 3 (Normal) 16, 22 -- X-MSMail-Priority: Normal 16, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 16, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 22 -- In-Reply-To: {200803260339.m2Q3dRhN029581-at-ns.microscopy.com} 16, 22 -- Importance: Normal 16, 22 -- X-OriginalArrivalTime: 26 Mar 2008 04:13:36.0149 (UTC) FILETIME=[C31B9450:01C88EF7] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z0chen09-at-louisville.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Research Scientist position on Transmission Electron Microscopy
Question: A Research Scientist position is available on materials chracterization by TEM, SEM, and XRD at Institute for Advanced Materials & Renewable Energy, University of Louisville. Please visit https://saprodic.louisville.edu/psc/saprod/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL to apply for the position.
The message below was forwarded to me for attention. It is not my area but I was hoping someone on the listserver would have an answer so that I can assist.
Many thanks.
Best wishes,
Colin
Colin Reid Senior Experimental Officer, Centre for Microscopy and Analysis (CMA), East End 4, Trinity College Dublin, Dublin 2.
Could a spare a moment of your time, i am trying to identify mono-sodium urate crystals (gout) in the laboratory.
I have a polarising set-up on the microscope and this is working okay for the bi-refringence, i want to use a filter to help distinguish between gout / pseudo-gout crystals.
I am led to believe that this is either a first order red filter or a lambda plate, is this correct are they the same thing? A company has quoted over a 1000 euros for a first order filter and polarising set, previously i have used a filter that sat ontop of the lower polariser do you know if this is an option (which i presume would be a lot cheaper).
Thanks for your help --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 14, 41 -- From creid-at-tcd.ie Wed Mar 26 02:28:55 2008 14, 41 -- Received: from outbound7-dub-R.bigfish.com (outbound-dub.frontbridge.com [213.199.154.16]) 14, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2Q7SaA6020244 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 02:28:39 -0500 14, 41 -- Received: from outbound7-dub.bigfish.com (localhost.localdomain [127.0.0.1]) 14, 41 -- by outbound7-dub-R.bigfish.com (Postfix) with ESMTP id 4A5915D0BEB 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 07:28:29 +0000 (UTC) 14, 41 -- Received: from mail17-dub-R.bigfish.com (mail17-dub [10.5.7.14]) 14, 41 -- by outbound7-dub.bigfish.com (Postfix) with ESMTP id 353FB898043 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 07:28:29 +0000 (UTC) 14, 41 -- Received: from mail17-dub (localhost.localdomain [127.0.0.1]) 14, 41 -- by mail17-dub-R.bigfish.com (Postfix) with ESMTP id D3AEC1110337 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 07:28:28 +0000 (UTC) 14, 41 -- X-BigFish: VP 14, 41 -- X-MS-Exchange-Organization-Antispam-Report: OrigIP: 134.226.1.156;Service: EHS 14, 41 -- Received: by mail17-dub (MessageSwitch) id 1206516508714264_6371; Wed, 26 Mar 2008 07:28:28 +0000 (UCT) 14, 41 -- Received: from imx2.tcd.ie (wpad.iss.tcd.ie [134.226.1.156]) 14, 41 -- by mail17-dub.bigfish.com (Postfix) with ESMTP id 37CFD1B5805E 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 07:28:28 +0000 (UTC) 14, 41 -- Received: from Vams.imx2 (imx2.tcd.ie [134.226.1.156]) 14, 41 -- by imx2.tcd.ie (Postfix) with SMTP id 27BFA68003 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 07:28:28 +0000 (GMT) 14, 41 -- Received: from imx2.tcd.ie ([134.226.1.156]) 14, 41 -- by imx2.tcd.ie ([134.226.1.156]) 14, 41 -- with SMTP (gateway) id A07EEB24352; Wed, 26 Mar 2008 07:28:28 +0000 14, 41 -- Received: from CMA135064.tcd.ie (cma135064.em-unit.tcd.ie [134.226.135.64]) 14, 41 -- by imx2.tcd.ie (Postfix) with ESMTP id 1553368003 14, 41 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Mar 2008 07:28:28 +0000 (GMT) 14, 41 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 41 -- Date: Wed, 26 Mar 2008 07:30:16 +0000 14, 41 -- To: Microscopy-at-Microscopy.Com 14, 41 -- From: Colin Reid {creid-at-tcd.ie} 14, 41 -- Subject: Mono-sodium urate crystals 14, 41 -- Mime-Version: 1.0 14, 41 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 14, 41 -- Message-Id: {20080326072828.1553368003-at-imx2.tcd.ie} 14, 41 -- X-AntiVirus-Status: MessageID = A17EEB24352 14, 41 -- X-AntiVirus-Status: Host: imx2.tcd.ie 14, 41 -- X-AntiVirus-Status: Action Taken: 14, 41 -- X-AntiVirus-Status: NONE 14, 41 -- X-AntiVirus-Status: Checked by TCD Vexira. (version=1.57.6 VDF=9.123.21) ==============================End of - Headers==============================
} In general, do you see the push for smaller instruments as a } positive move forward, or a quick "Bells and whistles" } attraction to earn new revenue?
Personally, I believe the smaller SEMs are only intended to fill a niche. That is, I don't see any of them really competing with the resolving power capable of the larger SEMs (altho I haven't yet seen anything about this new JEOL). Perhaps the better question is this inherently true, or is there a real possibility of smaller SEMs imaging as well, and being as versatile(?) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 4, 30 -- From michael-at-shaffer.net Wed Mar 26 06:01:35 2008 4, 30 -- Received: from smtprelay.hostedemail.com (smtprelay0149.hostedemail.com [216.40.44.149]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QB1XNQ017809 4, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 06:01:35 -0500 4, 30 -- Received: from emd2-omf04.hostedemail.com (ff-bigip1 [10.5.19.254]) 4, 30 -- by smtprelay03.hostedemail.com (Postfix) with ESMTP id A64433F9E3; 4, 30 -- Wed, 26 Mar 2008 11:01:32 +0000 (UTC) 4, 30 -- X-SpamScore: 50 4, 30 -- X-Spamcatcher-Summary: 50,0,0,0da6c8bcb77348fb,d630ebdca8644581,michael-at-shaffer.net,-,RULES_HIT:10:355:379:481:539:541:542:599:601:945:967:973:988:989:1155:1156:1160:1260:1277:1311:1313:1314:1345:1359:1437:1515:1516:1518:1534:1539:1593:1594:1711:1730:1747:1766:1792:2075:2078:2378:2393:2525:2560:2563:2682:2685:2691:2857:2859:2933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3352:3636:3865:3866:3867:3868:3869:3870:3871:3873:3874:3934:3936:3938:3941:3944:5007:6261:7679,0,RBL:none, 4, 30 -- CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 4, 30 -- X-Spamcatcher-Explanation: 4, 30 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 4, 30 -- (Authenticated sender: michael-at-shaffer.net) 4, 30 -- by emd2-omf04.hostedemail.com (Postfix) with ESMTP; 4, 30 -- Wed, 26 Mar 2008 11:01:32 +0000 (UTC) 4, 30 -- From: "michael shaffer" {michael-at-shaffer.net} 4, 30 -- To: {kraftpiano-at-gmail.com} 4, 30 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 4, 30 -- References: {200803260334.m2Q3Yckf023812-at-ns.microscopy.com} 4, 30 -- Subject: RE: [Microscopy] Table top SEM trend? 4, 30 -- Date: Wed, 26 Mar 2008 08:40:02 -0230 4, 30 -- Message-ID: {001701c88f31$f1452870$8d829986-at-CREAIT.MUN.CA} 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; 4, 30 -- charset="us-ascii" 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- X-Mailer: Microsoft Office Outlook 11 4, 30 -- Thread-Index: AciO8lIO3a3ol5oWS7O3Fs2dQF8ppgAPtajQ 4, 30 -- In-Reply-To: {200803260334.m2Q3Yckf023812-at-ns.microscopy.com} 4, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I need to embed small metal samples for polishing purposes. At the moment I have Leica brand Historesin kit available. It consists of basic resin, activator and hardener. Instructions says that activator should be kept away from heavy metal compounds. That is why I'm not sure if it will be okay to use the mixture for embedding metal samples, including heavy metal alloys.
Kind Regards, Ayten Celik-Aktas.
==============================Original Headers============================== 3, 27 -- From celikaktas-at-gmail.com Wed Mar 26 07:00:20 2008 3, 27 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.185]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QC0HGt000518 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 07:00:18 -0500 3, 27 -- Received: by fk-out-0910.google.com with SMTP id 18so5439335fkq.2 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 05:00:17 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=EhIFxNSVeapLHVPjbXUUZkas4t+zzyXTBB1HKoxGM1Q=; 3, 27 -- b=VWRAZ4Yin0evV6OH7uY/wuiMQy4dBUxZv556OEuSFvdYWI7s77a2tpYws5pxf2nriSwLHrP28z12dQAVPbPY8ukt1cPmEKUnwAPv+8BCS9UD2RZS99bKkDyAnZ/SLTdvd2/VRwJzDYd1ymReiPSUY8Hr6K8cRgl8Pm+7C1X7WYY= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=n55eIKmd/bZptNIqX9x4b7H42Oha9/+9o+b9/01wUjUhHOKPljenSbmueQBUS2f48VaaK5ioHaPez1tA1hXTjBFVSBiOtmlSYnTUe4MYeFdg4gNwTMCkPpVwUnAyykUFrP9HMhy1QmY9LsRW2AGykglPYs2wANtdeIEgoaItNhU= 3, 27 -- Received: by 10.82.134.12 with SMTP id h12mr24108294bud.34.1206532816831; 3, 27 -- Wed, 26 Mar 2008 05:00:16 -0700 (PDT) 3, 27 -- Received: by 10.82.125.20 with HTTP; Wed, 26 Mar 2008 05:00:16 -0700 (PDT) 3, 27 -- Message-ID: {1075c5c10803260500x4465cc2cr8d43e03af858a217-at-mail.gmail.com} 3, 27 -- Date: Wed, 26 Mar 2008 14:00:16 +0200 3, 27 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- Subject: Embedding metal samples using hitoresin 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=UTF-8 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
"Evex Mini-SEM (Scanning Electron Microscope and X-ray NanoAnalysis System)"
A table top. Add it to JEOL and Hitachi.
I suspect there will be more and more - for microscope users as opposed to microscopists. As the price comes down, expect to see more - for quickly checking/screening production of materials and electronic/medical devices, welding schools and welds in general, on-site WMD response, etc.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
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-----Original Message----- X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net] Sent: Wednesday, March 26, 2008 7:09 AM To: ph2-at-sprynet.com
Justin A. Kraft asks:
} In general, do you see the push for smaller instruments as a } positive move forward, or a quick "Bells and whistles" } attraction to earn new revenue?
Personally, I believe the smaller SEMs are only intended to fill a niche. That is, I don't see any of them really competing with the resolving power capable of the larger SEMs (altho I haven't yet seen anything about this new JEOL). Perhaps the better question is this inherently true, or is there a real possibility of smaller SEMs imaging as well, and being as versatile(?) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 4, 30 -- From michael-at-shaffer.net Wed Mar 26 06:01:35 2008 4, 30 -- Received: from smtprelay.hostedemail.com (smtprelay0149.hostedemail.com [216.40.44.149]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QB1XNQ017809 4, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 06:01:35 -0500 4, 30 -- Received: from emd2-omf04.hostedemail.com (ff-bigip1 [10.5.19.254]) 4, 30 -- by smtprelay03.hostedemail.com (Postfix) with ESMTP id A64433F9E3; 4, 30 -- Wed, 26 Mar 2008 11:01:32 +0000 (UTC) 4, 30 -- X-SpamScore: 50 4, 30 -- X-Spamcatcher-Summary: 50,0,0,0da6c8bcb77348fb,d630ebdca8644581,michael-at-shaffer.net,-,RULES_HIT:10: 355:379:481:539:541:542:599:601:945:967:973:988:989:1155:1156:1160:1260:1277 :1311:1313:1314:1345:1359:1437:1515:1516:1518:1534:1539:1593:1594:1711:1730: 1747:1766:1792:2075:2078:2378:2393:2525:2560:2563:2682:2685:2691:2857:2859:2 933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3352:3636:3865:3866:3867:38 68:3869:3870:3871:3873:3874:3934:3936:3938:3941:3944:5007:6261:7679,0,RBL:no ne, 4, 30 -- CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 4, 30 -- X-Spamcatcher-Explanation: 4, 30 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 4, 30 -- (Authenticated sender: michael-at-shaffer.net) 4, 30 -- by emd2-omf04.hostedemail.com (Postfix) with ESMTP; 4, 30 -- Wed, 26 Mar 2008 11:01:32 +0000 (UTC) 4, 30 -- From: "michael shaffer" {michael-at-shaffer.net} 4, 30 -- To: {kraftpiano-at-gmail.com} 4, 30 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 4, 30 -- References: {200803260334.m2Q3Yckf023812-at-ns.microscopy.com} 4, 30 -- Subject: RE: [Microscopy] Table top SEM trend? 4, 30 -- Date: Wed, 26 Mar 2008 08:40:02 -0230 4, 30 -- Message-ID: {001701c88f31$f1452870$8d829986-at-CREAIT.MUN.CA} 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; 4, 30 -- charset="us-ascii" 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- X-Mailer: Microsoft Office Outlook 11 4, 30 -- Thread-Index: AciO8lIO3a3ol5oWS7O3Fs2dQF8ppgAPtajQ 4, 30 -- In-Reply-To: {200803260334.m2Q3Yckf023812-at-ns.microscopy.com} 4, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From ph2-at-sprynet.com Wed Mar 26 07:49:41 2008 19, 28 -- Received: from elasmtp-junco.atl.sa.earthlink.net (elasmtp-junco.atl.sa.earthlink.net [209.86.89.63]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QCnfCB015372 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 07:49:41 -0500 19, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 19, 28 -- s=dk20050327; d=sprynet.com; 19, 28 -- b=CictjFQKnmx8ePqDuILPzQL3ygFn3I8bOS9zcKKvyGtJ2umbYoz2+ZQgUJfn8dtQ; 19, 28 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:In-Reply-To:Thread-Index:Message-ID:X-ELNK-Trace:X-Originating-IP; 19, 28 -- Received: from [75.61.18.94] (helo=user915fa8f284) 19, 28 -- by elasmtp-junco.atl.sa.earthlink.net with esmtpa (Exim 4.67) 19, 28 -- (envelope-from {ph2-at-sprynet.com} ) 19, 28 -- id 1JeV4W-0003Yg-PZ 19, 28 -- for microscopy-at-microscopy.com; Wed, 26 Mar 2008 08:49:41 -0400 19, 28 -- From: "Tony Havics" {ph2-at-sprynet.com} 19, 28 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 19, 28 -- Subject: RE: [Microscopy] RE: Table top SEM trend? 19, 28 -- Date: Wed, 26 Mar 2008 08:49:34 -0400 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="US-ASCII" 19, 28 -- Content-Transfer-Encoding: 7bit 19, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 19, 28 -- In-Reply-To: {200803261108.m2QB8aJ5025736-at-ns.microscopy.com} 19, 28 -- Thread-Index: AciPMb0rRjStwijDQ4eSyEbQgOryjgADFtAw 19, 28 -- Message-ID: {E1JeV4W-0003Yg-PZ-at-elasmtp-junco.atl.sa.earthlink.net} 19, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f94e134a07ebfcf3089b9f8d00cb0a8a4a350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 19, 28 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Don't forget the FEI product- we have JEOL, Hitachi, FEI, Evex. Should we start taking odds on who's next?
--Justin.
On Wed, Mar 26, 2008 at 8:57 AM, {ph2-at-sprynet.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Coincidence? } } I just received an email yesterday on: } } "Evex Mini-SEM (Scanning Electron Microscope and X-ray NanoAnalysis System)" } } A table top. Add it to JEOL and Hitachi. } } I suspect there will be more and more - for microscope users as opposed to } microscopists. As the price comes down, expect to see more - for quickly } checking/screening production of materials and electronic/medical devices, } welding schools and welds in general, on-site WMD response, etc. } } } Tony } } ...................................................................... } Andrew Anthony "Tony" Havics, CHMM, CIH, PE } pH2, LLC } 5250 E US 36, Suite 830 } Avon, IN 46123 } www.ph2llc.com } } (317) 718-7020 off } (317) 718-7038 fax } (317) 409-3238 cell } } 90% of Risk Management is knowing where to place the decimal point...any } consultant can give you the other 10%(SM) } } This message is from pH2. This message and any attachments may contain } legally privileged or confidential information, and are intended only for } the individual or entity identified above as the addressee. 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All personal messages express views only of } the sender, which are not to be attributed to pH2 and may not be copied or } distributed without this statement. } } -----Original Message----- } X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net] } Sent: Wednesday, March 26, 2008 7:09 AM } To: ph2-at-sprynet.com } Subject: [Microscopy] RE: Table top SEM trend? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin A. Kraft asks: } } } In general, do you see the push for smaller instruments as a } } positive move forward, or a quick "Bells and whistles" } } attraction to earn new revenue? } } Personally, I believe the smaller SEMs are only intended to fill a niche. } That is, I don't see any of them really competing with the resolving power } capable of the larger SEMs (altho I haven't yet seen anything about this new } JEOL). Perhaps the better question is this inherently true, or is there a } real possibility of smaller SEMs imaging as well, and being as versatile(?) } ~~~~~~~~~~~~~~~~~~~~~~~~~ } cheerios, michael shaffer :o) } SEM-MLA Research Coordinator } INCO Innovation Centre } Memorial University } St. John's Newfoundland } http://www.mun.ca/creait/maf/ } } } ==============================Original Headers============================== } 4, 30 -- From michael-at-shaffer.net Wed Mar 26 06:01:35 2008 } 4, 30 -- Received: from smtprelay.hostedemail.com } (smtprelay0149.hostedemail.com [216.40.44.149]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m2QB1XNQ017809 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 06:01:35 } -0500 } 4, 30 -- Received: from emd2-omf04.hostedemail.com (ff-bigip1 [10.5.19.254]) } 4, 30 -- by smtprelay03.hostedemail.com (Postfix) with ESMTP id } A64433F9E3; } 4, 30 -- Wed, 26 Mar 2008 11:01:32 +0000 (UTC) } 4, 30 -- X-SpamScore: 50 } 4, 30 -- X-Spamcatcher-Summary: } 50,0,0,0da6c8bcb77348fb,d630ebdca8644581,michael-at-shaffer.net,-,RULES_HIT:10: } 355:379:481:539:541:542:599:601:945:967:973:988:989:1155:1156:1160:1260:1277 } :1311:1313:1314:1345:1359:1437:1515:1516:1518:1534:1539:1593:1594:1711:1730: } 1747:1766:1792:2075:2078:2378:2393:2525:2560:2563:2682:2685:2691:2857:2859:2 } 933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3352:3636:3865:3866:3867:38 } 68:3869:3870:3871:3873:3874:3934:3936:3938:3941:3944:5007:6261:7679,0,RBL:no } ne, } 4, 30 -- } CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not } bulk,SPF:,MSBL:none,DNSBL:none } 4, 30 -- X-Spamcatcher-Explanation: } 4, 30 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca } [134.153.130.141]) } 4, 30 -- (Authenticated sender: michael-at-shaffer.net) } 4, 30 -- by emd2-omf04.hostedemail.com (Postfix) with ESMTP; } 4, 30 -- Wed, 26 Mar 2008 11:01:32 +0000 (UTC) } 4, 30 -- From: "michael shaffer" {michael-at-shaffer.net} } 4, 30 -- To: {kraftpiano-at-gmail.com} } 4, 30 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} } 4, 30 -- References: {200803260334.m2Q3Yckf023812-at-ns.microscopy.com} } 4, 30 -- Subject: RE: [Microscopy] Table top SEM trend? } 4, 30 -- Date: Wed, 26 Mar 2008 08:40:02 -0230 } 4, 30 -- Message-ID: {001701c88f31$f1452870$8d829986-at-CREAIT.MUN.CA} } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Content-Type: text/plain; } 4, 30 -- charset="us-ascii" } 4, 30 -- Content-Transfer-Encoding: 7bit } 4, 30 -- X-Mailer: Microsoft Office Outlook 11 } 4, 30 -- Thread-Index: AciO8lIO3a3ol5oWS7O3Fs2dQF8ppgAPtajQ } 4, 30 -- In-Reply-To: {200803260334.m2Q3Yckf023812-at-ns.microscopy.com} } 4, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 19, 28 -- From ph2-at-sprynet.com Wed Mar 26 07:49:41 2008 } 19, 28 -- Received: from elasmtp-junco.atl.sa.earthlink.net (elasmtp-junco.atl.sa.earthlink.net [209.86.89.63]) } 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QCnfCB015372 } 19, 28 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 07:49:41 -0500 } 19, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 19, 28 -- s=dk20050327; d=sprynet.com; } 19, 28 -- b=CictjFQKnmx8ePqDuILPzQL3ygFn3I8bOS9zcKKvyGtJ2umbYoz2+ZQgUJfn8dtQ; } 19, 28 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-MimeOLE:In-Reply-To:Thread-Index:Message-ID:X-ELNK-Trace:X-Originating-IP; } 19, 28 -- Received: from [75.61.18.94] (helo=user915fa8f284) } 19, 28 -- by elasmtp-junco.atl.sa.earthlink.net with esmtpa (Exim 4.67) } 19, 28 -- (envelope-from {ph2-at-sprynet.com} ) } 19, 28 -- id 1JeV4W-0003Yg-PZ } 19, 28 -- for microscopy-at-microscopy.com; Wed, 26 Mar 2008 08:49:41 -0400 } 19, 28 -- From: "Tony Havics" {ph2-at-sprynet.com} } 19, 28 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} } 19, 28 -- Subject: RE: [Microscopy] RE: Table top SEM trend? } 19, 28 -- Date: Wed, 26 Mar 2008 08:49:34 -0400 } 19, 28 -- MIME-Version: 1.0 } 19, 28 -- Content-Type: text/plain; } 19, 28 -- charset="US-ASCII" } 19, 28 -- Content-Transfer-Encoding: 7bit } 19, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 } 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 19, 28 -- In-Reply-To: {200803261108.m2QB8aJ5025736-at-ns.microscopy.com} } 19, 28 -- Thread-Index: AciPMb0rRjStwijDQ4eSyEbQgOryjgADFtAw } 19, 28 -- Message-ID: {E1JeV4W-0003Yg-PZ-at-elasmtp-junco.atl.sa.earthlink.net} } 19, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f94e134a07ebfcf3089b9f8d00cb0a8a4a350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c } 19, 28 -- X-Originating-IP: 75.61.18.94 } ==============================End of - Headers============================== }
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==============================Original Headers============================== 6, 29 -- From kraftpiano-at-gmail.com Wed Mar 26 08:01:16 2008 6, 29 -- Received: from rn-out-0910.google.com (rn-out-0910.google.com [64.233.170.186]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QD1Frx028093 6, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 08:01:16 -0500 6, 29 -- Received: by rn-out-0910.google.com with SMTP id a43so2495290rne.10 6, 29 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 06:01:15 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=beta; 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 29 -- bh=R3QRd4hlPH24FgeONfs6jUWuQXg7GH+F0MrggCkeM+I=; 6, 29 -- b=YDAH//ZmyrYWCfxHCC0luQAhzPGskTh8L7qsMj1Ht/tRLczZuJm82syXHLBqMDmgzuzjPViOSCxd+s8qx+TZJx0G+BYB1r5Lt0kpSDcNQCBTY3KN1PjUgQ/0plE6Av3rX9ynEeY2dNUnh/8SOY0BnuwAdymOs1oiLZL3ztprTx0= 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 29 -- d=gmail.com; s=beta; 6, 29 -- h=message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 29 -- b=FyIAe8qIRDAEAWB6lZrJFwZPc4M8UbLAK/vC0VLGMjTaQQ/NPEvEXToatGfwr7pMyzsyZQtapLbylW8cQgNbDuhSIDKgYQtwRsqHZmh6GhDejFNA5LtL9XFcvGfMtpxYlmMFRei/WtM5l2wpdWXtqxJSHd8g6ngc11BDY5QvkAg= 6, 29 -- Received: by 10.141.15.19 with SMTP id s19mr19264rvi.39.1206536473134; 6, 29 -- Wed, 26 Mar 2008 06:01:13 -0700 (PDT) 6, 29 -- Received: by 10.140.161.11 with HTTP; Wed, 26 Mar 2008 06:01:13 -0700 (PDT) 6, 29 -- Message-ID: {25e2b0d20803260601s7f84464s5dbd489e029b0f83-at-mail.gmail.com} 6, 29 -- Date: Wed, 26 Mar 2008 09:01:13 -0400 6, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 6, 29 -- To: ph2-at-sprynet.com, microscopy-at-microscopy.com 6, 29 -- Subject: Re: [Microscopy] Table top SEM trend? 6, 29 -- In-Reply-To: {200803261257.m2QCveVF024932-at-ns.microscopy.com} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- Content-Disposition: inline 6, 29 -- References: {200803261257.m2QCveVF024932-at-ns.microscopy.com} ==============================End of - Headers==============================
JEOL had a small SEM a gajillion years ago (OK, so it was only in the 1970s) I remember John Bonicci wanting to donate one to a high school when it was being decommissioned from whatever lab it had been in. It was of the same generation as the JSM 25...I have no recollection about how well or poorly it worked, I just remember that it existed. Lee -- Lee Cohen-Gould Electron & Optical Microscopy Core Facilities Weill Cornell Medical College (212)746-6146 Rms A-105, LC-207 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
==============================Original Headers============================== 1, 25 -- From lcgould-at-med.cornell.edu Wed Mar 26 08:29:35 2008 1, 25 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 1, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QDTZ4p009677 1, 25 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 08:29:35 -0500 1, 25 -- Received: from mpx2.med.cornell.edu (pc113142-13.med.cornell.edu [140.251.11.119]) 1, 25 -- by smtp-gw2.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m2QDT9IT017756 1, 25 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:29:34 -0400 (EDT) 1, 25 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu [140.251.48.23]) 1, 25 -- by mpx2.med.cornell.edu 1, 25 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 25 -- with ESMTPA id {0JYC00J8XASJN270-at-mpx2.med.cornell.edu} for 1, 25 -- microscopy-at-microscopy.com; Wed, 26 Mar 2008 09:29:09 -0400 (EDT) 1, 25 -- Date: Wed, 26 Mar 2008 09:29:03 -0400 1, 25 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} 1, 25 -- Subject: [Microscopy] Re: Table top SEM trend? 1, 25 -- In-reply-to: {200803261308.m2QD8Uq0006643-at-ns.microscopy.com} 1, 25 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} 1, 25 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 25 -- Message-id: {p06230902c40fff5ff418-at-[140.251.48.23]} 1, 25 -- MIME-version: 1.0 1, 25 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 25 -- Content-transfer-encoding: 7BIT 1, 25 -- References: {200803261308.m2QD8Uq0006643-at-ns.microscopy.com} 1, 25 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.26.61618 1, 25 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_500_599 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
I think the ISI Mini SEM ( now Topcon) would have been the original of the species ? I used one for a short period in 1980. Maximum magnification, as I recall, was about X4K on a good day. Tiny CRT which required a tap on the side from time to time to stabilise the image. We still have negatives here from it. I know there were a few around at the time. The column/chamber was about 50cm high. Those were the days ?
Colin
At 13:36 26/03/2008, you wrote:
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I am looking for advice on purchasing of our first SEM. We received funds to construct a new 1,500 sq. ft. Class 1000 clean room and lab area and I am tasked with the responsibility of purchasing and finding donated equipment for the lab area. My first purchase will be a SEM and my experience is very limited. Because of limited funding we can only purchase one SEM and it will need to be capable of doing both material characteriztion and for use in our biosciences area. I realize this is a compromise. Given that we are a 2-year community college we will not be doing cutting edge R&D. Is there one SEM that can reasonably handle both tasks? It will be used primarily in a training environment and for use by outside companies in the area on a contract basis.
I'm leaning towards the Hitachi S-3500N. Any advice would be greatly appreciated.
CRAIG L. ANDERSON Saint Paul College Minnesota State Colleges & Universities System Voice: 651.846.1365 Fax: 651.846.1675
==============================Original Headers============================== 5, 20 -- From Craig.Anderson-at-saintpaul.edu Wed Mar 26 09:04:16 2008 5, 20 -- Received: from spc-mail.saintpaul.edu (mail.saintpaul.edu [204.77.52.74]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QE4G3p003517 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:04:16 -0500 5, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 20 -- Content-class: urn:content-classes:message 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" 5, 20 -- Subject: Advice on purchasing our first SEM 5, 20 -- Date: Wed, 26 Mar 2008 09:02:37 -0500 5, 20 -- Message-ID: {F3210C938F1D4243912CB54A6037F112D4B68B-at-spc-mail.saintpaul.edu} 5, 20 -- X-MS-Has-Attach: 5, 20 -- X-MS-TNEF-Correlator: 5, 20 -- Thread-Topic: Advice on purchasing our first SEM 5, 20 -- Thread-Index: AciPSgxcNLlMAwRcRIGsSjrc0S9azQ== 5, 20 -- From: "Craig Anderson Ext 1365" {Craig.Anderson-at-saintpaul.edu} 5, 20 -- To: {microscopy-at-microscopy.com} 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2QE4G3p003517 ==============================End of - Headers==============================
I got a glimpse of one of those a couple of years ago when I got my first TEM. It was sitting in an adjacent room but I didn't have time to study it and they didn't want to give it to me either... I had to settle for a free TEM. It's a hard world... :-)
It looked neat, I almost wish I got that instead of the TEM.
Göran
lcgould-at-med.cornell.edu wrote: } JEOL had a small SEM a gajillion years ago (OK, so it was only in the } 1970s) I remember John Bonicci wanting to donate one to a high } school when it was being decommissioned from whatever lab it had been } in. It was of the same generation as the JSM 25...I have no } recollection about how well or poorly it worked, I just remember that } it existed. } Lee }
==============================Original Headers============================== 5, 24 -- From axelsson-at-acc.umu.se Wed Mar 26 09:27:18 2008 5, 24 -- Received: from mail.acc.umu.se (mail.acc.umu.se [130.239.18.156]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QERHNn016911 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:27:18 -0500 5, 24 -- Received: from localhost (localhost [127.0.0.1]) 5, 24 -- by amavisd-new (Postfix) with ESMTP id 1CF1C83 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 15:27:17 +0100 (MET) 5, 24 -- X-Virus-Scanned: amavisd-new at acc.umu.se 5, 24 -- Received: from [192.168.1.4] (1-1-10-48a.um.um.bostream.se [82.182.239.55]) 5, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 24 -- (No client certificate requested) 5, 24 -- by mail.acc.umu.se (Postfix) with ESMTP id 811377C 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 15:27:06 +0100 (MET) 5, 24 -- Message-ID: {47EA5D36.1040203-at-acc.umu.se} 5, 24 -- Date: Wed, 26 Mar 2008 15:27:02 +0100 5, 24 -- From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= {axelsson-at-acc.umu.se} 5, 24 -- User-Agent: Thunderbird 2.0.0.6 (X11/20071022) 5, 24 -- MIME-Version: 1.0 5, 24 -- To: microscopy-at-microscopy.com 5, 24 -- Subject: Re: [Microscopy] Re: Table top SEM trend? 5, 24 -- References: {200803261329.m2QDTiEn009753-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {200803261329.m2QDTiEn009753-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I think the trend towards microscopic microscopes is going to continue. Back in 1998-1999 there were a series of papers about minute SEMs and electron sources: Dean and Chalamala, Applied Physics Letters, 1999 75(18):3017 on "The environmental stability of field emission from single-walled carbon nanotubes." Driskill-Smisth et al., Applied Physics Letters, 1999 75(18):2845 on "The 'nano-triode': A nanoscale field-emission tube." George, NASA Tech Briefs, August:25-26 on a electron microscope column a "few milimeters thick and about a centimeter square." All of which could add up to a FESEM small enough to require a light microscope to see it. Actually, I'm surprised there isn't an Apple iScan by now.
Phil
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==============================Original Headers============================== 3, 22 -- From oshel1pe-at-cmich.edu Wed Mar 26 09:39:47 2008 3, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QEdkVO029728 3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:39:47 -0500 3, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m2QErp9V001847 3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 10:53:59 -0400 3, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 3, 22 -- Wed, 26 Mar 2008 10:39:45 -0400 3, 22 -- Mime-Version: 1.0 3, 22 -- Message-Id: {f06240808c4100e2d53a6-at-[141.209.160.249]} 3, 22 -- In-Reply-To: {200803261352.m2QDqrNk029151-at-ns.microscopy.com} 3, 22 -- References: {200803261352.m2QDqrNk029151-at-ns.microscopy.com} 3, 22 -- Date: Wed, 26 Mar 2008 10:39:39 -0400 3, 22 -- To: Microscopy-at-microscopy.com 3, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 22 -- Subject: [Microscopy] Re: Re: Table top SEM trend? 3, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 22 -- X-OriginalArrivalTime: 26 Mar 2008 14:39:45.0743 (UTC) FILETIME=[3C54ADF0:01C88F4F] 3, 22 -- X-CanItPRO-Stream: default 3, 22 -- X-Spam-Score: -4 () L_EXCH_MF 3, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Hi Craig, I don't know the Hitachi instruments all that well, but the FEI Quanta 200 FEG ESEM instrument we bought two years ago is the most versatile, easy to use instrument I have found so far in my career. The BSE detector is large (18mm) so gives excellent BSE images for geology, the ESEM mode is perfect for biological (and other insulating materials), in high vac mode it has 3 to 4 nm resolution which is decent for materials applications and it has a reasonably long (10mm) working distance that makes it safe for all kinds of odd shaped samples and also useable with EDS and CL without changing the sample height.
Contact me directly if you'd like more details. john
At 07:12 AM 3/26/2008, you wrote:
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==============================Original Headers============================== 7, 25 -- From donovan-at-uoregon.edu Wed Mar 26 09:59:52 2008 7, 25 -- Received: from QMTA09.westchester.pa.mail.comcast.net (qmta09.westchester.pa.mail.comcast.net [76.96.62.96]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QExp9B010362 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:59:52 -0500 7, 25 -- Message-Id: {200803261459.m2QExp9B010362-at-ns.microscopy.com} 7, 25 -- Received: from OMTA07.westchester.pa.mail.comcast.net ([76.96.62.59]) 7, 25 -- by QMTA09.westchester.pa.mail.comcast.net with comcast 7, 25 -- id 5qRJ1Z03Z1GhbT85901M00; Wed, 26 Mar 2008 14:58:21 +0000 7, 25 -- Received: from SOURCE.uoregon.edu ([71.193.189.123]) 7, 25 -- by OMTA07.westchester.pa.mail.comcast.net with comcast 7, 25 -- id 5qzo1Z0052gBFvr3T00000; Wed, 26 Mar 2008 14:59:51 +0000 7, 25 -- X-Authority-Analysis: v=1.0 c=1 a=9uT894auGtgA:10 a=Zx37jsudAAAA:8 7, 25 -- a=VnlNNZAcMOVsZqMuc40A:9 a=q2EVoi7AQHf8trPqfBAA:7 7, 25 -- a=Tv5wKexsf0FeqfonZQUhEfXlJXYA:4 a=G91thX30KR8A:10 a=2TDmcdGY2UwA:10 7, 25 -- a=Byk4NIk2KzMA:10 a=50e4U0PicR4A:10 7, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 25 -- Date: Wed, 26 Mar 2008 07:59:51 -0700 7, 25 -- To: Craig.Anderson-at-saintpaul.edu 7, 25 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 7, 25 -- Subject: Re: [Microscopy] Advice on purchasing our first SEM 7, 25 -- Cc: microscopy-at-microscopy.com 7, 25 -- In-Reply-To: {200803261412.m2QEC5wi011755-at-ns.microscopy.com} 7, 25 -- References: {200803261412.m2QEC5wi011755-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
You are much better off than myself....I have yet had time to even get the plastic wrapping off the latest Microscopy Today.
In response to the last part of the post:
"In general, do you see the push for smaller instruments as a positive move forward, or a quick "Bells and whistles" attraction to earn new revenue?"
Positive move forward. I'm a firm believer these table top microscopes will be key in interesting the younger generation in science, technology, engineering and mathematics (STEM). The SEM images are easy to interpret and open up a whole new landscape where the students can be discoverers of new patterns and morphology on the micro to nano scale!
Another benefit of the table top SEM, with a small footprint and ease of use, is that it is finding use in different disciplines. For example design science. Students at the Pratt Institute Design School are using a table top SEM (under the instruction of Jonas Coersmeier) as a way to observe patterns on the micro and nanoscale that can be used in the design of an aquatic center. There progress can be seen on the blog www.probelog.com/sem/ or www.probelog.com/span and the goal of the table top SEM enabled design project is
"it is important that they clearly define which particular tectonic/structural/morphological characteristics they are interested in per group/project, so they do not randomly search for cool images but focus on specific effects. I want them to have established a taxonomy for their image collection (in terminology and diagram), so the material is put in context and processed effectively in analytical drawings and generative models ('nanotectonica') In general I want them to establish an expertise that is based on specific charactersitica that can be found in different areas within the vast field of natural samples."
The price tag of the table top SEMs are probably attractive to smaller companies. The reality I struggle with is that schools, (middle, high school) still do not have access to these instruments for educational activities. Maybe getting a few of the microscopes into museums and bringing them to legislature offices to have our representatives actually use them may be a good way to get some help with state and federal funds which would lower the price tag so more schools can use them.
It is a bit harsh to assume that the manufacturers only want to earn new revenue. I routinely think about the amount of engineering and labor that goes into making these instruments and want to thank all EM manufacturers for making my workday fun and go much faster. Besides, where would science be without these tools to "see" things synthesized in the lab and acquire materials properties on the nanoscale?
Anywho, just my 2 cents.
Donovan
kraftpiano-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I just got my new copy of Microscopy Today in the mail, and have } proceeded my usual course of devouring it, when I cam across an } advertisement for a new tabletop SEM from JEOL. Are tabletop SEMs the } new bandwagon product for manufacturers to be producing now? I also } saw an email regarding an Evex tabletop analytical model. } } Also in this issue is a paper on a smaller TEM made possible by } improved cooling methods. } } With all of this miniaturization, I'm just curious what everyone } thinks about the possibility of having SEMs and TEMs small enough to } take into the field (Eventually)? } } In general, do you see the push for smaller instruments as a positive } move forward, or a quick "Bells and whistles" attraction to earn new } revenue? } } Just curious, } } Justin A. Kraft } }
==============================Original Headers============================== 13, 32 -- From donovan.leonard-at-gmail.com Wed Mar 26 10:16:55 2008 13, 32 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.168]) 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QFGsew023562 13, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 10:16:54 -0500 13, 32 -- Received: by wf-out-1314.google.com with SMTP id 23so3887545wfg.21 13, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 08:16:53 -0700 (PDT) 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 32 -- d=gmail.com; s=beta; 13, 32 -- h=domainkey-signature:received:received:message-id:date:from:user-agent:mime-version:to:subject:references:in-reply-to:content-type:content-transfer-encoding; 13, 32 -- bh=lKTy9hrt2uNzc2SMfsc645O4XBEmjxt+u4ynzkpCNP4=; 13, 32 -- b=ri4ObSpOSs5Ojqhjirhqq3PvsLzB+O1CAFDCQSiErhbfyKqMRl4wQkhSk/nr312jnvMH46UbV53ol2EsRHB/KFzFEPv3l3S3laLzB12YYn8kxTXGnEErWX7RCCg8Qz1kOypi5MZsa1Dvzag1VthPr+9FBfblvJOD9CXIbaz3ZWw= 13, 32 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 32 -- d=gmail.com; s=beta; 13, 32 -- h=message-id:date:from:user-agent:mime-version:to:subject:references:in-reply-to:content-type:content-transfer-encoding; 13, 32 -- b=HL9aNqxz9NIQuUs2rAxVwUtM4OQpOON6vOLJbUDfj6Zd0++iP4dK2dkEXbcEPLoMeDNi1XU4Gb3w1y0RUWVssY8Mwx8e5ahjL6uNfyCqkI/lifq15Ob7A9lZwl3V5fKTp2mDcMMV9J1iis7ZvIraT8WgGgOY/9Zs+G1+TIxZyiQ= 13, 32 -- Received: by 10.143.33.19 with SMTP id l19mr247321wfj.18.1206544612334; 13, 32 -- Wed, 26 Mar 2008 08:16:52 -0700 (PDT) 13, 32 -- Received: from ip-152010152199.phys.appstate.edu ( [152.10.152.199]) 13, 32 -- by mx.google.com with ESMTPS id 9sm14816199wrl.31.2008.03.26.08.16.51 13, 32 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 13, 32 -- Wed, 26 Mar 2008 08:16:51 -0700 (PDT) 13, 32 -- Message-ID: {47EA68E2.3010400-at-gmail.com} 13, 32 -- Date: Wed, 26 Mar 2008 11:16:50 -0400 13, 32 -- From: Donovan N Leonard {donovan.leonard-at-gmail.com} 13, 32 -- User-Agent: Thunderbird 2.0.0.12 (Macintosh/20080213) 13, 32 -- MIME-Version: 1.0 13, 32 -- To: Microscopy-at-microscopy.com 13, 32 -- Subject: Re: [Microscopy] Table top SEM trend? 13, 32 -- References: {200803260341.m2Q3f4gu031666-at-ns.microscopy.com} 13, 32 -- In-Reply-To: {200803260341.m2Q3f4gu031666-at-ns.microscopy.com} 13, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The question of whether there is a tabletop trend remains to be seen. What is certain, however, is that the technology to significantly reduce SEM (and TEM...read on) size has drastically improved due to innovations in electron optics and vacuum technologies.
A real trend will develop if commercially available equipment continues to meet users needs. The equipment should provide all the benefits of a smaller (table top) footprint, without trading off imaging results.
There is no question that there is a renaissance of benchtop SEM, as FEI, Hitachi and now JEOL have introduced benchtop SEM with ~ 20K magnification in the past few years...clearly they believe that there is a market demand.
Here at Delong (DISCLAIMER - I WORK FOR DELONG AND WE MAKE BENCHTOP TEM AND SEM), we've felt that way for a long time. We introduced our benchtop TEM-SEM a few years back, and our main innovations (aside from sharing the benchtop footprint with the other manufacturers) was - and remains - operations at a low 5 kV and the use of a FEG which is why we have 3 nm or better resolution (more than 200K magnification in SEM) in both our TEM and SEM modes.
Prior introductions of the technology in decades past were probably a little rushed. Today's benchtop SEM (and TEM) are better (both in terms of specifications and reliability), more user friendly and a more economical alternative for researchers to meet their imaging needs.
So, it appears that it has the makings of a trend. Time will tell.
Ephram
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-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Tuesday, March 25, 2008 11:43 PM To: msa-at-lv-em.com
I just got my new copy of Microscopy Today in the mail, and have proceeded my usual course of devouring it, when I cam across an advertisement for a new tabletop SEM from JEOL. Are tabletop SEMs the new bandwagon product for manufacturers to be producing now? I also saw an email regarding an Evex tabletop analytical model.
Also in this issue is a paper on a smaller TEM made possible by improved cooling methods.
With all of this miniaturization, I'm just curious what everyone thinks about the possibility of having SEMs and TEMs small enough to take into the field (Eventually)?
In general, do you see the push for smaller instruments as a positive move forward, or a quick "Bells and whistles" attraction to earn new revenue?
Just curious,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 7, 27 -- From kraftpiano-at-gmail.com Tue Mar 25 22:34:02 2008 7, 27 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.227]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2Q3Y2P2023202 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 22:34:02 -0500 7, 27 -- Received: by wx-out-0506.google.com with SMTP id h30so4872047wxd.21 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Mar 2008 20:34:01 -0700 (PDT) 7, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 27 -- d=gmail.com; s=beta; 7, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime -version:content-type:content-transfer-encoding:content-disposition; 7, 27 -- bh=vOrNr8SN6aULxK3i4pvVLM0gpObvTC1G/VUI1AKmJ28=; 7, 27 -- b=uCirNn3/Yv2PzDv3ZMQK9YiA585EDUwens0WElfmd59zltcLagSD5fBBwGtZRrDdIspUWmeXfF UvDjtQ2xbJJPlqXepm3enFY9phvDAFfenFkbj1Ud45SKqwCuInM78ilszicH1jwhjRMU7xCm7c19 IezmEPON5Qh9dh17GW+PE= 7, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 27 -- d=gmail.com; s=beta; 7, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer -encoding:content-disposition; 7, 27 -- b=UwQtwLMN19o1pAajIkqc5SUTkenwRPpe0j9bJMawUlW2uK43wiT+yDfq9x8IzpZ3b5fB1/Oexn cu35juy+GrePwpHZnDMThkwrpRFLrwnw8CBmeRorKLytIfkBqEk+OuJG3IlvUjbyqddIgT/s2POp d4p8oYfIW+Qe5jHOCOSZA= 7, 27 -- Received: by 10.140.133.10 with SMTP id g10mr4057463rvd.170.1206502440870; 7, 27 -- Tue, 25 Mar 2008 20:34:00 -0700 (PDT) 7, 27 -- Received: by 10.140.161.11 with HTTP; Tue, 25 Mar 2008 20:34:00 -0700 (PDT) 7, 27 -- Message-ID: {25e2b0d20803252034k3e389d71hb24de980093ee645-at-mail.gmail.com} 7, 27 -- Date: Tue, 25 Mar 2008 23:34:00 -0400 7, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 7, 27 -- To: microscopy-at-microscopy.com 7, 27 --
Dear Craig, I purchased the Hitachi S-3000N a few years ago and have found it a very versatile, valuable instrument for all manner of samples. The S-3000N has the same column and electronics as the S3500N, but the chamber is a bit smaller and the price is lower. The chamber is still large enough for a four-inch square sample. The students use it all day for materials characterization, bio-materials and all manner of university, research-type samples. It is now six years old and has not yet had a service call, except when I upgraded the computer to Windows XP. We also got the 5-axis motorized stage, which is very helpful. Regards,
-----Original Message----- X-from: Craig.Anderson-at-saintpaul.edu [mailto:Craig.Anderson-at-saintpaul.edu] Sent: March 26, 2008 7:14 AM To: maryflet-at-interchange.ubc.ca
I am looking for advice on purchasing of our first SEM. We received funds to construct a new 1,500 sq. ft. Class 1000 clean room and lab area and I am tasked with the responsibility of purchasing and finding donated equipment for the lab area. My first purchase will be a SEM and my experience is very limited. Because of limited funding we can only purchase one SEM and it will need to be capable of doing both material characteriztion and for use in our biosciences area. I realize this is a compromise. Given that we are a 2-year community college we will not be doing cutting edge R&D. Is there one SEM that can reasonably handle both tasks? It will be used primarily in a training environment and for use by outside companies in the area on a contract basis.
I'm leaning towards the Hitachi S-3500N. Any advice would be greatly appreciated.
CRAIG L. ANDERSON Saint Paul College Minnesota State Colleges & Universities System Voice: 651.846.1365 Fax: 651.846.1675
==============================Original Headers============================== 5, 20 -- From Craig.Anderson-at-saintpaul.edu Wed Mar 26 09:04:16 2008 5, 20 -- Received: from spc-mail.saintpaul.edu (mail.saintpaul.edu [204.77.52.74]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QE4G3p003517 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:04:16 -0500 5, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 20 -- Content-class: urn:content-classes:message 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" 5, 20 -- Subject: Advice on purchasing our first SEM 5, 20 -- Date: Wed, 26 Mar 2008 09:02:37 -0500 5, 20 -- Message-ID: {F3210C938F1D4243912CB54A6037F112D4B68B-at-spc-mail.saintpaul.edu} 5, 20 -- X-MS-Has-Attach: 5, 20 -- X-MS-TNEF-Correlator: 5, 20 -- Thread-Topic: Advice on purchasing our first SEM 5, 20 -- Thread-Index: AciPSgxcNLlMAwRcRIGsSjrc0S9azQ== 5, 20 -- From: "Craig Anderson Ext 1365" {Craig.Anderson-at-saintpaul.edu} 5, 20 -- To: {microscopy-at-microscopy.com} 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2QE4G3p003517 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 36 -- From maryflet-at-interchange.ubc.ca Wed Mar 26 11:11:04 2008 15, 36 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5]) 15, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QGB3ZX018562 15, 36 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 11:11:04 -0500 15, 36 -- X-Ubc-Received: from mr3.mail-relay.ubc.ca (localhost [127.0.0.1]) 15, 36 -- by localhost (Postfix) with SMTP id A492AB7DB 15, 36 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:11:03 -0700 (PDT) 15, 36 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 15, 36 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP 15, 36 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 09:11:02 -0700 (PDT) 15, 36 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 15, 36 -- by smtp.interchange.ubc.ca 15, 36 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 15, 36 -- with ESMTPS id {0JYC002YEIAEFC-at-smtp.interchange.ubc.ca} for 15, 36 -- microscopy-at-microscopy.com; Wed, 26 Mar 2008 09:11:02 -0700 (PDT) 15, 36 -- Date: Wed, 26 Mar 2008 09:10:32 -0700 15, 36 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 15, 36 -- Subject: RE: [Microscopy] Advice on purchasing our first SEM 15, 36 -- In-reply-to: {200803261414.m2QEETAb013837-at-ns.microscopy.com} 15, 36 -- To: Craig.Anderson-at-saintpaul.edu 15, 36 -- Cc: microscopy-at-microscopy.com 15, 36 -- Reply-to: maryflet-at-interchange.ubc.ca 15, 36 -- Message-id: {0JYC002YFIAEFC-at-smtp.interchange.ubc.ca} 15, 36 -- Organization: Materials Eng. 15, 36 -- MIME-version: 1.0 15, 36 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 15, 36 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 15, 36 -- Content-type: text/plain; charset=iso-8859-1 15, 36 -- Thread-index: AciPS7TgYr2whlifSNe5dqBGwMHWDAAD1oDg 15, 36 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.3.26.85733 15, 36 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 15, 36 -- X-PerlMx-Spam: Probability=7%, Report=OEM_SOFTWARE_X1 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0 15, 36 -- X-Spam-Level: 15, 36 -- X-Spam-Flag: No 15, 36 -- Content-Transfer-Encoding: 8bit 15, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2QGB3ZX018562 ==============================End of - Headers==============================
I'm having a problem with Ultracut S series (Leica/Reichert) ultra-microtome. None of the displays light on the control box when it is switched on, nor do any of the buttons control their functions on the microtome.
The fuse checks out OK and our electrician tested the power-supply and found it to be functional. He can't really do any more without a circuit diagram. Does anyone have this information that could share it with us?
Specifics: Ultracut S control box with the following ID from the nameplate.
Type: 654901 Serial #: 89439
Thanks, Jay
Jay Campbell University of Wisconsin Dept of Biochemistry 433 Babcock Dr Madison, WI 53706
608 890 0219 microtomy-at-gmail.com
==============================Original Headers============================== 9, 27 -- From microtomy-at-gmail.com Wed Mar 26 12:41:04 2008 9, 27 -- Received: from wf-out-1314.google.com (wf-out-1314.google.com [209.85.200.171]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QHf1xn003634 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 12:41:03 -0500 9, 27 -- Received: by wf-out-1314.google.com with SMTP id 23so3947054wfg.21 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 10:41:00 -0700 (PDT) 9, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 27 -- d=gmail.com; s=beta; 9, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 9, 27 -- bh=jiWyhhfqZmyoe3kIcMs5WgUxLFL7xM58MXYYfdHc+1E=; 9, 27 -- b=ZIr3r2QCBeHqlyyoybq8BHnMWARRrJkf7AO8q97lhMrOJxbkwUQiVSXYffDDOEZjmlP0olxCe3Z6D8e1TurNYEK8e26DSmK4tHVW0Ist1sVCnmix5g+CwBAh/R5B0V2hj/UqiN51mqNtuDK1XbaV9L3Oa6iKiqwV+JrnHQBT25k= 9, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 9, 27 -- d=gmail.com; s=beta; 9, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 9, 27 -- b=WbO2fUd1vAXTDD6sfs+r6HmX98rJkTEgn0WvQUPZKykFGiNeTwoHjvbJwGkX4OoOe6PsFINXp1C+pOMeyKaDeByqfea28FbJeg90aPTJYaDhqZ29+0uWIesSOB5xWne3S1AAdZGIfF7Xr9lb0enwbIVYfPn3TH3cawEeKxIja0E= 9, 27 -- Received: by 10.142.179.12 with SMTP id b12mr462551wff.88.1206553260962; 9, 27 -- Wed, 26 Mar 2008 10:41:00 -0700 (PDT) 9, 27 -- Received: by 10.143.36.14 with HTTP; Wed, 26 Mar 2008 10:41:00 -0700 (PDT) 9, 27 -- Message-ID: {ef6bda5c0803261041w41e64002k36ed80e184e22b48-at-mail.gmail.com} 9, 27 -- Date: Wed, 26 Mar 2008 12:41:00 -0500 9, 27 -- From: "Jay Campbell" {microtomy-at-gmail.com} 9, 27 -- To: microscopy {microscopy-at-microscopy.com} 9, 27 -- Subject: Ultracut S microtome problem 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; charset=ISO-8859-1 9, 27 -- Content-Transfer-Encoding: 7bit 9, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Hi Tom There are two ways that I have used. One involves serial sectioning though cells (Elliott in Microscopy Today Jan 2007) and reconstructing the cells. That way you know how many/how large/what shape the vacuoles are (Elliott et al, PNAS 2008). The second way is to use the well worked out tools of Stereology. The book I like for this is "Unbiased Stereology - Three-Dimensional Measurement in Microscopy" which is available from Amazon for about $18. Feel free to contact me if you would like to talk.
David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Mar 25, 2008, at 4:40 PM, tbargar-at-unmc.edu wrote: } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } Same project, with two questions. } } I am doing TEM on several cell cultures. The investigator is sure } that some } cell lines "appear" to have many vacuoles and other cell lines } "appear" to } have few vacuoles or none. The investigator would like me to } quantify } this condition. My question is how does one quantify this in such } a way as } to be statistically meaningful? } } Secondly, the investigator feels that some cell lines have more } mitochondria, while other cell lines have fewer mitochondria. } Again they } would like to get quantitative data of this condition. What are } people's } thoughts on how to approach this question? } } If any respondent is really curious as to what I have already } tried to } explain to the investigator please include your telephone number } and I will } gladly call you. All help is appreciated. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original } Headers============================== } 7, 20 -- From tbargar-at-unmc.edu Tue Mar 25 18:36:48 2008 } 7, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu } [192.198.54.127]) } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m2PNamLN001969 } 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 } 18:36:48 -0500 } 7, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) } 7, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id F3ED3A0060 } 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 } 18:36:47 -0500 (CDT) } 7, 20 -- Received: from unmcnotes01.unmc.edu } (host-137-197-103-35.unmc.edu [137.197.103.35]) } 7, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id B2F56398060 } 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 } 18:36:46 -0500 (CDT) } 7, 20 -- Subject: Vacuolated cells vs. non vacuolated cells } 7, 20 -- To: Microscopy-at-MSA.Microscopy.com } 7, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 } 7, 20 -- Message-ID: {OF76288709.261A67AE- } ON86257417.007F5CB5-86257417.0081B55E-at-unmc.edu} } 7, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} } 7, 20 -- Date: Tue, 25 Mar 2008 18:36:45 -0500 } 7, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/ } Servers/UNEBR at 03/25/2008 06:36:46 } 7, 20 -- PM } 7, 20 -- MIME-Version: 1.0 } 7, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - } Headers============================== }
==============================Original Headers============================== 8, 22 -- From Elliott-at-arizona.edu Wed Mar 26 16:07:46 2008 8, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2QL7kc1026200 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 26 Mar 2008 16:07:46 -0500 8, 22 -- Received: from gandalfs_amavis (amavis3.email.arizona.edu [10.0.0.206]) 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id BF31A544FC3 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 26 Mar 2008 14:07:43 -0700 (MST) 8, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2F36C544FC0 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 26 Mar 2008 14:07:39 -0700 (MST) 8, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 8, 22 -- In-Reply-To: {200803252340.m2PNe93o006582-at-ns.microscopy.com} 8, 22 -- References: {200803252340.m2PNe93o006582-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 22 -- Message-Id: {0104EA3E-BA6A-4C0F-B99C-4A1DDFF7E3BD-at-arizona.edu} 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- From: David Elliott {Elliott-at-arizona.edu} 8, 22 -- Subject: Re: [Microscopy] Vacuolated cells vs. non vacuolated cells 8, 22 -- Date: Wed, 26 Mar 2008 14:07:38 -0700 8, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 8, 22 -- X-Mailer: Apple Mail (2.753) 8, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Has anyone proved the specs of your system? How about the other desk top systems. I don't believe the claims. If this was so fabulous, all the other SEM makers would not be selling their tools and no one would be buying them...IMO.
The mix of "nano" in this theme is way off target. With a system that works up to 20KX, 65A oxide is not going to be seen. Try to use this system for SWCNT or MWCNT. No can do.
I think the simple SEMs are in a good position for specific applications. That is the same for the higher power FEGSEMs. There are specific application areas and therefore, the necessity of tools that meet that application.
Of course, YMMV.
gary g.
At 08:49 AM 3/26/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Wed Mar 26 20:50:43 2008 11, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2R1ohiT016705 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 26 Mar 2008 20:50:43 -0500 11, 20 -- Message-Id: {200803270150.m2R1ohiT016705-at-ns.microscopy.com} 11, 20 -- Received: (qmail 1510 invoked from network); 26 Mar 2008 18:50:42 -0700 11, 20 -- Received: by simscan 1.1.0 ppid: 1507, pid: 1508, t: 0.2204s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp2 with SMTP; 26 Mar 2008 18:50:42 -0700 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Wed, 26 Mar 2008 18:50:41 -0700 11, 20 -- To: msa-at-lv-em.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Desktop SEM 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200803261549.m2QFn0sg007957-at-ns.microscopy.com} 11, 20 -- References: {200803261549.m2QFn0sg007957-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-391AB0 ==============================End of - Headers==============================
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Email: gxx-at-u.washington.edu Name: Xiaoxia
Organization: The University of Washington
Title-Subject: [Filtered] Glass knife maker
Question: In our nanotechnology center, we have a brand new leica EM UC6 microtome, and plan to buy a glass maker to make glass knife used in the Leica microtome.I had some experience with LKB 7800 knife maker, however, i couldn't find any retailer for this products( I did find some used one on sale). Does this brand not exsit any more? Or any people knows there is other knife maker could make the same knife(trangular knife).
Your microscopes are far too big and specialist for ebay here in the UK (although in the US similar beasts are regularly sold on-line and so there is an active ebay auction market there – even I’ve bought obsolete microscope spares from there occasionally). But auctions are a gamble - I've seen identical 1860s JB Dancer micro-photographic slides go for £350 and as little as £25 [although it's more difficult to counterfeit a Zeiss Universal]. The sales pitch on ebay, positive feedback, and auction time planning are crucial.
These universals of yours need a lot of room – ‘my’ old Zeiss Universals at Harwell were monsters, and I remember at the MRC our photographer’s grey Ultraphot II looked like a Minbari battleship, parked in it’s own little room.
Normally I’d suggest contact an amateur microscope club (they aren't as common as the night sky telescope crowd but there's plenty about) – they might not have the pockets (cash) though. There are also specialist on-line microscope stores that sell on this type of kit, probably to labs, but I doubt they will offer you top dollar as they need to make a profit, but it might be $3,000 to $5,000 or so, as it appears they can try and sell up to $8,000 or more, see below) - view them on-line and just ask to get a guide to price (all the extras like fluorescence, objective set and phase contrast would have added to it's price years ago and so affect it’s resale value). Your Universals are in another league to most portable lab microscopes, and that limits the potential buyers (as most private buyers couldn’t afford them or the wife might move out as the microscope moved in). With fluorescence and Hg lamps it’s not really suitable for schools, but a 16+ college might be very interested. I have seen a few very nice ‘modern’ 1980s Leitz Laborlux phase contrast photo-microscopes for around £1,5000 (without fluorescence) on ebay, and I wouldn’t have thought that the Universal was any more desirable than one of these - at home I just use an old British Cooke, Troughton & Simms of York microscope, that’s only 14 inches high with a large mirror for illumination and built like a precision dreadnought [with pretty decent optics up to 600x mag, oil immersion, but no phase], but then it cost next to nothing.
http://www.buntgrp.com/used_microscopes.htm
You could ask these online microscope shop guys how much they are selling theirs for if it’s not shown (I suspect it’s well over two thousand dollars for a fluorescence Universal - but it depends on the number on the market, condition and what’s fitted on the microscope, and whether there’s any buyers): http://www.microscopestore.com/Used_Specialty_13_136.html http://www.niscorp.us/specials_-_Zeiss_Universal_Microscope.htm
The discussion at http://sci.tech-archive.net/Archive/sci.techniques.microscopy/2006-09/msg00062.html is relevant (suggesting up to $8,000 for well fitted out Universal back in 2006 - but thats via a retail store not a private seller).
Also try the workplace if you are involved in science, as many scientists may have an interest in buying a ‘cheap’ quality microscope for the lab. Those in the countryside will probably have more free bench-top space than us city Boys.
These microscopes aren't decorative in-home items like pretty little brass ones from the 1860s - they are big, industrial, expensive and are only suitable for enthusiasts/scientists/students who want to use them regularly. Some hospital/science museums/institutes may like them as well though as display items if yours are in poor condition optically (Zeiss can still clean them up for you, but I doubt they can offer anything in hardware terms other than a photocopy/pdf of the original manuals that’s worth having). If the internal optics seem fine, I'd leave any maintenance to the buyer, unless you are knowledgeable about cleaning external microscope optics.
These Universals might be difficult items to shift due to their intrinsic value (assuming they are working perfectly), design style/age and bulk, but there are prospective buyers out there, and I suspect in these days of on-line selling/buying they will be fairly easy to find.
X-from memory, the Ultraphot II is such a large odd-looking beast I don’t know if that will be so easy to shift now that the microscope imaging media of the colour slide is all but dead (although fitting a modern digital camera shouldn’t be impossible). If only you knew the names of a few world famous scientists who might have used it.
Would I pay $8,000+ for a Zeiss Universal? – well I think I could have got my old one for free eight years ago when our lab was shut down, but we decided it was just too large, heavy and complicated for our local school [it had a motorized XY stage], so probably not – ours was eventually given away to another lab locally, [as it wasn’t our money if we sold it anyway]. If these are your personal property it would probably be worthwhile investing time selling them. Although asking up to $8,000 doesn’t mean you’ll get it – but if you can prove it’s as good as new, who knows. I've never actually sold one though, but at least I found I could easily give a few away - accepter collects [even a massive Bio-Rad 1024 laser confocal microscope, no longer supported by Zeiss Microscience, was gratefully accepted as a freebie by a nearby institute recently].
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
-----Original Message----- X-from: ben.vandenberg-at-valeinco.com [mailto:ben.vandenberg-at-valeinco.com] Sent: 26 March 2008 04:13 To: kjmorris-at-well.ox.ac.uk
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Email: ben.vandenberg-at-valeinco.com Name: Ben Vanden Berg
Title-Subject: [Filtered] LM Equipment, Zeiss, Standard Universal and Ultraphot II
Question: I have 3 microscopes which have been collecting dust for years and I would like to sell or donate
Does anyone know the value of the following items and the best place to auction equipment like this?
All microscopes are compound models, Zeiss, 1960ís vintage
1 standard universal, polarizing, transmitted light equipment for reflected light 1 standard universal, polarizing, transmitted light, florescence and photography equipped 1 ultraphot II, monster photography machine numerous accessories
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
The Microscopy Society of the Ohio River Valley (MSORV) will be having it's Spring Meeting on Wednesday April 9, 2008, 3-7PM, at the Fawcett Center at The Ohio State University in Columbus, OH.
This is one you don't want to miss! We are very fortunate to be able to have an MAS Tour Speaker presenting at this meeting along with three of our colleagues. 1) MAS Tour Speaker - Louis T. Germinario, Eastman Chemical Company, Corporate Analytical Services, Kingsport, TN, 37662. "Nanoscale Thermal Property Characterization of Polymers, Thin Films and Coatings"
2) Angela Blissett, The Ohio State University, Columbus, OH. "Regulation of Collagen Fibrillogenesis by Kinase-Dead DDR2". Co-authors: Derek Garbellini, Ed Calomeni, Cosmin Mihai, Terry Elton and Gunjan Agarwal.
3) Arda Genc, The Ohio State University, Columbus, OH. "New Experiences in Aberration Corrected Electron Microscopy".
4) Jim Waldman, The Ohio State University, Columbus, OH "Microscopy in the Molecular Age: STILL a Tool for Discovery in Virology"
Directions to the Fawcett Center and the complete agenda are located on our website: www.msorv.org
RSVP: By March 31, 2008 to pamela.lloyd-at-wpafb.af.mil
Although there is no registration fee for this meeting, it is required that you Pre-register.
Hope to see you there!
Pamela F. Lloyd MSORV Secretary
==============================Original Headers============================== 12, 30 -- From Pamela.Lloyd-at-WPAFB.AF.MIL Thu Mar 27 05:30:38 2008 12, 30 -- Received: from fohfwa005.oh.afmc.af.mil (fohfwa005.oh.afmc.af.mil [131.28.29.204]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2RAUctL009624 12, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2008 05:30:38 -0500 12, 30 -- Received: from FOHMLRL04.enterprise.afmc.ds.af.mil (fohmlrl04.enterprise.afmc.ds.af.mil [131.28.34.158]) 12, 30 -- by fohfwa005.oh.afmc.af.mil with ESMTP id m2RAUcJu010182 12, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2008 10:30:38 GMT 12, 30 -- X-AuditID: 831c229e-00001ea400000164-a2-47eb774d5615 12, 30 -- Received: from FOHMLBH03.Enterprise.afmc.ds.af.mil ([10.1.1.1]) by FOHMLRL04.enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.1830); 12, 30 -- Thu, 27 Mar 2008 06:30:37 -0400 12, 30 -- Received: from VFOHMLAO13.Enterprise.afmc.ds.af.mil ([131.28.34.135]) by FOHMLBH03.Enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.2942); 12, 30 -- Thu, 27 Mar 2008 06:30:37 -0400 12, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 30 -- Content-class: urn:content-classes:message 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; 12, 30 -- charset="us-ascii" 12, 30 -- Subject: Spring Meeting - MSORV (Microscopy Society of the Ohio River Valley) 12, 30 -- Date: Thu, 27 Mar 2008 06:30:37 -0400 12, 30 -- Message-ID: {29A7C5F65D54744CBFD97CD62A33DEE90301D57C-at-VFOHMLAO13.Enterprise.afmc.ds.af.mil} 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- Thread-Topic: Spring Meeting - MSORV (Microscopy Society of the Ohio River Valley) 12, 30 -- Thread-Index: AciP9ZixXOq7Ai8QQWGxtw9/HhB7Pg== 12, 30 -- From: "Lloyd, Pamela F CTR USAF AFMC AFRL/RXPJ" {Pamela.Lloyd-at-WPAFB.AF.MIL} 12, 30 -- To: {Microscopy-at-microscopy.com} 12, 30 -- X-OriginalArrivalTime: 27 Mar 2008 10:30:37.0677 (UTC) FILETIME=[990091D0:01C88FF5] 12, 30 -- X-Brightmail-Tracker: AAAAAA== 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2RAUctL009624 ==============================End of - Headers==============================
If you want to "go microscopic", I agree that stereology is probably the way to go, but I don't think that you need to spend the time and energy (and expertise) for 3D reconstruction. In the end you just want to know if the vacuoles are more numerous/bigger, you don't really care to know exactly their volume.
You have the chance to work with cells in culture, which means that the cell population is relatively homogenous. Cutting through a cell pellet or through flat-embedded monolayers will give you plenty of cells to count and measure with joy and excitement. Flat-embedding would probably offer the advantage that most of the cells in one sample will be cut at the same level and the cell area (in sections) offered for analysis will be in the same order. Flat-embedding also preserves the cell morphology in situ. However to embed and cut monolayers is a little more complicated than to collect and pellet the cells. Another problem with flat-embedding is that you may cut right where the vacuoles are in one sample and in the other, cut in a part of the cell layer where the vacuoles are not. In this regard cutting through pellets will give you fully randomized cutting directions in all cells.
The principle here is to photograph randomly or systematically a lot of cells, to measure the total cell surface and the surface of the compartment of interest (vacuole for example) and to make statistics. The statistics will tell you if your result is significant or not. The good news is that you can quantify both the vacuoles AND mitochondria with the same set of pictures. It would probably be worthwhile to collect their number as well as their surface (which could give you the mean surface for example.). If you are not familiar with stereology it would be worthwhile to read some book because this technique, like others, has its pitfalls and one can easily produce biased results. However, once correctly performed, it is pretty easy.
This the EM solution.
Another MUCH faster solution, but I have no experience in this field, would be to find a fluorescent dye for vacuoles and mitochondria, and to FACS the cells. I am confident it should be pretty straightforward, just talk with people of the field. Look by Molecular Probes for organelle-specific dyes, they are not the cheapest but their have really good products (no interest here).
Best regards,
Stephane
P.S: working as a microscopist does not dispense from thinking about solutions outside the field of microscopy. Yes, there is reasearch outside microscopy! (although we all agree it is not as satisfying as microscopy :-D)
----- Original Message ---- X-from: "tbargar-at-unmc.edu" {tbargar-at-unmc.edu} To: nizets2-at-yahoo.com Sent: Wednesday, March 26, 2008 12:41:38 AM
Same project, with two questions.
I am doing TEM on several cell cultures. The investigator is sure that some cell lines "appear" to have many vacuoles and other cell lines "appear" to have few vacuoles or none. The investigator would like me to quantify this condition. My question is how does one quantify this in such a way as to be statistically meaningful?
Secondly, the investigator feels that some cell lines have more mitochondria, while other cell lines have fewer mitochondria. Again they would like to get quantitative data of this condition. What are people's thoughts on how to approach this question?
If any respondent is really curious as to what I have already tried to explain to the investigator please include your telephone number and I will gladly call you. All help is appreciated.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 7, 20 -- From tbargar-at-unmc.edu Tue Mar 25 18:36:48 2008 7, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2PNamLN001969 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 18:36:48 -0500 7, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 7, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id F3ED3A0060 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 18:36:47 -0500 (CDT) 7, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 7, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id B2F56398060 7, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 25 Mar 2008 18:36:46 -0500 (CDT) 7, 20 -- Subject: Vacuolated cells vs. non vacuolated cells 7, 20 -- To: Microscopy-at-MSA.Microscopy.com 7, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 7, 20 -- Message-ID: {OF76288709.261A67AE-ON86257417.007F5CB5-86257417.0081B55E-at-unmc.edu} 7, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 7, 20 -- Date: Tue, 25 Mar 2008 18:36:45 -0500 7, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 03/25/2008 06:36:46 7, 20 -- PM 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
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==============================Original Headers============================== 24, 20 -- From nizets2-at-yahoo.com Thu Mar 27 06:53:42 2008 24, 20 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 24, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2RBrghQ025373 24, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2008 06:53:42 -0500 24, 20 -- Received: (qmail 92105 invoked by uid 60001); 27 Mar 2008 11:53:41 -0000 24, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 24, 20 -- s=s1024; d=yahoo.com; 24, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 24, 20 -- b=fu49lrFFSvXHT4vElAVwMSLmT/D+vYfraozb13g3DIT3CxZ80gQDnUNN1g5V66agxNr4vZVjbw9hOOV9GRmy9iiY6lz+OgahUhULdc/zzD0Z1IsD3fT4NQYDcE0upTgSH9bteg3XX0d+apky/eMjsnWrA7j4KFaEEfUTbj70oPE=; 24, 20 -- X-YMail-OSG: HZnb9r4VM1kjbKldKO9rGoNa8ePWWFkObJVbLGpFQNTIW6G8H4rTAWYJV1jau3.EHCMskaShSJzQJT5g.w5b735yyaDg__2s_U0nYpw.krMmutR4P.PJIZd0E..ngSFtYu.rgDzMEGXJupgKjIongfnYZw9t33lK05UA_swIb8P_m_Jq6gX_B2I- 24, 20 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Thu, 27 Mar 2008 04:53:41 PDT 24, 20 -- X-Mailer: YahooMailRC/902.40 YahooMailWebService/0.7.185 24, 20 -- Date: Thu, 27 Mar 2008 04:53:41 -0700 (PDT) 24, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 24, 20 -- Subject: Re: [Microscopy] Vacuolated cells vs. non vacuolated cells 24, 20 -- To: tbargar-at-unmc.edu 24, 20 -- Cc: microscopy-at-microscopy.com 24, 20 -- MIME-Version: 1.0 24, 20 -- Content-Type: text/plain; charset=us-ascii 24, 20 -- Message-ID: {615690.90907.qm-at-web37408.mail.mud.yahoo.com} ==============================End of - Headers==============================
We bought our glass knife maker from RMC. It uses the balanced break method. I find it less intimidating to set up then the LKB models I have used in the past.
Cheers, KD Derr New York Structural Biology Center
-----Original Message----- X-from: gxx-at-u.washington.edu [mailto:gxx-at-u.washington.edu] Sent: Thursday, March 27, 2008 12:13 AM To: kderr-at-nysbc.org
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both gxx-at-u.washington.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: gxx-at-u.washington.edu Name: Xiaoxia
Organization: The University of Washington
Title-Subject: [Filtered] Glass knife maker
Question: In our nanotechnology center, we have a brand new leica EM UC6 microtome, and plan to buy a glass maker to make glass knife used in the Leica microtome.I had some experience with LKB 7800 knife maker, however, i couldn't find any retailer for this products( I did find some used one on sale). Does this brand not exsit any more? Or any people knows there is other knife maker could make the same knife(trangular knife).
As the physical size gets smaller the maximum voltage you can use on a vacuum tube gets lower and lower due to the smaller distance between elements in the tube and the lower the voltage required to arc over these distances. In the end an electron microscope is only a very specialized vacuum tube.
Nuvistors about the smallest production vacuum tube ever made. http://www.thevalvepage.com/valvetek/Nuvistor/nuvistor.htm All I know of had had low plate voltages. The in the example 7895 had 110 volt specification on the plate. It would probably work at 200 or 300 volts DC and fail in spectacular fashion in a few hours. Most vacuum tubes can be run at 2 or 3 times their rated voltage and current with added cooling at the price of a dramatic shortening of their life.
I have seen circuits with Nuvistors run with plate voltages as low as 12 volts. Using 12, 24 and 42 volts on the plate of Nuvistors is common.
If one was going to experiment with diminutive SEMs a Nuvistor wouldseem to me to be a good place to start. With a little surgery theyshould give you an cheap repeatable election source.
If you can get useful information from a SEM operated at low voltages making a diminutive SEM with a corresponding reduction in the field of view is probable possible and safe to do. No one is likely to get hurt very bad with less than 200 volts at the low current levels SEM operate.
If anyone wan't to ty thirei had a making one I think I can find a hand full of Nuvistors around here some place or find some one that does.
Best Wishes Gordon Couger Stillwater OK www.science-info.net {http://www.science-info.net}
I think the trend towards microscopic microscopes is going to continue. Back in 1998-1999 there were a series of papers about minute SEMs and electron sources: Dean and Chalamala, Applied Physics Letters, 1999 75(18):3017 on "The environmental stability of field emission from single-walled carbon nanotubes." Driskill-Smisth et al., Applied Physics Letters, 1999 75(18):2845 on "The 'nano-triode': A nanoscale field-emission tube." George, NASA Tech Briefs, August:25-26 on a electron microscope column a "few milimeters thick and about a centimeter square." All of which could add up to a FESEM small enough to require a light microscope to see it. Actually, I'm surprised there isn't an Apple iScan by now.
Phil
==============================Original Headers============================== 16, 18 -- From gcouger-at-science-info.net Thu Mar 27 11:24:23 2008 16, 18 -- Received: from smtp102.biz.mail.mud.yahoo.com (smtp102.biz.mail.mud.yahoo.com [68.142.200.237]) 16, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m2RGOMiE030095 16, 18 -- for {microscopy-at-microscopy.com} ; Thu, 27 Mar 2008 11:24:23 -0500 16, 18 -- Received: (qmail 76390 invoked from network); 27 Mar 2008 16:24:22 -0000 16, 18 -- Received: from unknown (HELO gordon-cougers-computer.local) (gcouger-at-science-info.net-at-74.195.225.38 with plain) 16, 18 -- by smtp102.biz.mail.mud.yahoo.com with SMTP; 27 Mar 2008 16:24:22 -0000 16, 18 -- X-YMail-OSG: Y57FLokVM1mRpoEcBUt3I5VGwAgKBPuWhawI6326QXOnTksFqEe5Lw3pfW6bLHIWQDPpSUmYpMKAqPHx3NJcQaehWTz4DNw3hq4peG.Teg4wwNCO6ew- 16, 18 -- X-Yahoo-Newman-Property: ymail-3 16, 18 -- Message-ID: {47EBCA34.9030007-at-science-info.net} 16, 18 -- Date: Thu, 27 Mar 2008 11:24:20 -0500 16, 18 -- From: Gordon Couger {gcouger-at-science-info.net} 16, 18 -- User-Agent: Thunderbird 2.0.0.12 (Macintosh/20080213) 16, 18 -- MIME-Version: 1.0 16, 18 -- To: microscopy-at-microscopy.com 16, 18 -- Subject: Re: [Microscopy] Table top SEM trend? 16, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Neat stuff. The articles referenced, though, where actually being used to propose an "on chip" SEM. Very low voltage would be a benefit, as it would make the "nanoguns" essentially surface instruments with potentially high resolution. Using a carbon nanotube as a field-emitter would eliminate the need to demagnify a virtual source, and would give a very small spot-size at the sample. Check out T. George's article. The real problem would be the same one that delayed development of the SEM in the first place: detecting the secondaries with a reasonable signal-noise ratio. There was a note related to this in the Oct 2000 Microscopy Today.
Phil
} Hi Phil, } } As the physical size gets smaller the maximum voltage you can use on } a vacuum tube gets lower and lower due to the smaller distance } between elements in the tube and the lower the voltage required to } arc over these distances. In the end an electron microscope is only } a very specialized vacuum tube. } } Nuvistors about the smallest production vacuum tube ever made. } http://www.thevalvepage.com/valvetek/Nuvistor/nuvistor.htm All I } know of had had low plate voltages. The in the example 7895 had 110 } volt specification on the plate. It would probably work at 200 or } 300 volts DC and fail in spectacular fashion in a few hours. Most } vacuum tubes can be run at 2 or 3 times their rated voltage and } current with added cooling at the price of a dramatic shortening of } their life. } } I have seen circuits with Nuvistors run with plate voltages as low } as 12 volts. Using 12, 24 and 42 volts on the plate of Nuvistors is } common. } } If one was going to experiment with diminutive SEMs a Nuvistor } wouldseem to me to be a good place to start. With a little surgery } theyshould give you an cheap repeatable election source. } } If you can get useful information from a SEM operated at low } voltages making a diminutive SEM with a corresponding reduction in } the field of view is probable possible and safe to do. No one is } likely to get hurt very bad with less than 200 volts at the low } current levels SEM operate. } } If anyone wan't to ty thirei had a making one I think I can find a } hand full of Nuvistors around here some place or find some one that } does. } } Best Wishes } Gordon Couger } Stillwater OK } www.science-info.net } } } From: "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu} } } I think the trend towards microscopic microscopes is going to } continue. Back in 1998-1999 there were a series of papers about } minute SEMs and electron sources: } Dean and Chalamala, Applied Physics Letters, 1999 75(18):3017 on "The } environmental stability of field emission from single-walled carbon } nanotubes." } Driskill-Smisth et al., Applied Physics Letters, 1999 75(18):2845 on } "The 'nano-triode': A nanoscale field-emission tube." } George, NASA Tech Briefs, August:25-26 on a electron microscope } column a "few milimeters thick and about a centimeter square." } All of which could add up to a FESEM small enough to } require a light } microscope to see it. } Actually, I'm surprised there isn't an Apple iScan by now. } } Phil }
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 23 -- From oshel1pe-at-cmich.edu Thu Mar 27 12:30:40 2008 5, 23 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2RHUeCW013274 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Mar 2008 12:30:40 -0500 5, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 23 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m2RHii9Z018862; 5, 23 -- Thu, 27 Mar 2008 13:44:49 -0400 5, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 5, 23 -- Thu, 27 Mar 2008 13:30:41 -0400 5, 23 -- Mime-Version: 1.0 5, 23 -- Message-Id: {f06240804c411882428be-at-[141.209.160.249]} 5, 23 -- In-Reply-To: {862482.5138.qm-at-web410.biz.mail.mud.yahoo.com} 5, 23 -- References: {862482.5138.qm-at-web410.biz.mail.mud.yahoo.com} 5, 23 -- Date: Thu, 27 Mar 2008 13:30:34 -0400 5, 23 -- To: "gordon.couger-at-gmail.com" {gordon.couger-at-gmail.com} 5, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 23 -- Subject: Re: [Microscopy] Table top SEM trend? 5, 23 -- Cc: Microscopy-at-microscopy.com 5, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 23 -- X-OriginalArrivalTime: 27 Mar 2008 17:30:41.0263 (UTC) FILETIME=[478297F0:01C89030] 5, 23 -- X-CanItPRO-Stream: default 5, 23 -- X-Spam-Score: -3.5 () L_EXCH_MF,TO_ADDRESS_EQ_REAL 5, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Leica-microsystems makes the latest version of the LKB Knifemaker, along with all supplies and accessories. They also provide maintenance, parts, etc.
You can find it at: leica-microsystems.com/EM_Specimen_Prep
} Email: gxx-at-u.washington.edu } Name: Xiaoxia } } Organization: The University of Washington } } Title-Subject: [Filtered] Glass knife maker } } Question: In our nanotechnology center, we have a brand new leica EM } UC6 microtome, and plan to buy a glass maker to make glass knife used } in the Leica microtome.I had some experience with LKB 7800 knife } maker, however, i couldn't find any retailer for this products( I did } find some used one on sale). Does this brand not exsit any more? Or } any people knows there is other knife maker could make the same } knife(trangular knife).
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: microscopy-at-marigoldresearch.com Name: Marilyn Dwyer
Organization: Marigold Mgt & Research (Retained Search)
Question: Our F500 Life Sciences client is seeking a direct hire Product Manager for Electron Microscopy, located in Madison WI. Relocation.
If you can act as the voice of the customer and provide strategic direction in the development and implementation of specifications for new products, this might be the next career move for you. This person will have responsibility for outbound focused marketing and support the x-ray microanalysis portfolio including Product Positioning, Market Segmentation, and Pricing Structures. Travel.
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Hi I am flat embedding some very thin biological samples (they are grown on aclar film and embedded). Normally it is very easy to find the sample, it is on the edge of the plastic.
For some of what I am doing, after I remove the aclar film I put more epon in its place and incubate it again. This works very well to protect the biology that is right at the air/epon interface. The difficulty is that it can be VERY hard to find the biology.
I was wondering if I would color the epon (we are using Embed-812 - EM Science (#14120)) so that it would be easy to find the line between the colored and uncolored epon?
Thank you David
_________________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 8, 20 -- From Elliott-at-Arizona.edu Fri Mar 28 13:15:43 2008 8, 20 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2SIFhoV003909 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 13:15:43 -0500 8, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 494E7554F4F 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 11:15:43 -0700 (MST) 8, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 0B555554F4C 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 11:15:43 -0700 (MST) 8, 20 -- Mime-Version: 1.0 (Apple Message framework v753) 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- Message-Id: {29BD405C-88B3-415F-B8B4-A3A3CB5563DB-at-Arizona.edu} 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 8, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 8, 20 -- From: David Elliott {Elliott-at-Arizona.edu} 8, 20 -- Subject: coloring epon 8, 20 -- Date: Fri, 28 Mar 2008 11:15:41 -0700 8, 20 -- X-Mailer: Apple Mail (2.753) 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I remember reading (but have never tried myself) that for specimens that are sandwich embedded between aclar sheets, one can cut out the specimens that are good and rub a black wax pencil over the edges of the wafer before re-embedding. I can't remember where I read this, though. Maybe something from Peter Vesk or Jeremy Pickett-Heaps or more recently, in MT? In your case, you might be able to circle the specimen?
Andy
________________________ Andrew J. Bowling, Ph.D. Research Plant Physiologist USDA - ARS - SWSRU 141 Experiment Station Rd. Stoneville, MS 38776 (662)686-5285
-----Original Message----- X-from: Elliott-at-Arizona.edu [mailto:Elliott-at-Arizona.edu] Sent: Friday, March 28, 2008 12:26 PM To: Bowling, Andrew
Hi I am flat embedding some very thin biological samples (they are grown on aclar film and embedded). Normally it is very easy to find the sample, it is on the edge of the plastic.
For some of what I am doing, after I remove the aclar film I put more epon in its place and incubate it again. This works very well to protect the biology that is right at the air/epon interface. The difficulty is that it can be VERY hard to find the biology.
I was wondering if I would color the epon (we are using Embed-812 - EM Science (#14120)) so that it would be easy to find the line between the colored and uncolored epon?
Thank you David
_________________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 8, 20 -- From Elliott-at-Arizona.edu Fri Mar 28 13:15:43 2008 8, 20 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2SIFhoV003909 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 13:15:43 -0500 8, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 494E7554F4F 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 11:15:43 -0700 (MST) 8, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 0B555554F4C 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 11:15:43 -0700 (MST) 8, 20 -- Mime-Version: 1.0 (Apple Message framework v753) 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- Message-Id: {29BD405C-88B3-415F-B8B4-A3A3CB5563DB-at-Arizona.edu} 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 8, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 8, 20 -- X-from: David Elliott {Elliott-at-Arizona.edu} 8, 20 -- Subject: coloring epon 8, 20 -- Date: Fri, 28 Mar 2008 11:15:41 -0700 8, 20 -- X-Mailer: Apple Mail (2.753) 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
==============================Original Headers============================== 20, 34 -- From Andrew.Bowling-at-ARS.USDA.GOV Fri Mar 28 14:05:34 2008 20, 34 -- Received: from messagescreen2.ars.usda.gov (messagescreen2.ars.usda.gov [199.133.30.201]) 20, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2SJ5YMm020566 20, 34 -- for {microscopy-at-microscopy.com} ; Fri, 28 Mar 2008 14:05:34 -0500 20, 34 -- Received: from messagescreen2.ars.usda.gov (localhost [127.0.0.1]) 20, 34 -- by messagescreen2.ars.usda.gov (8.13.8/8.13.8) with ESMTP id m2SJ5StE012878 20, 34 -- for {microscopy-at-microscopy.com} ; Fri, 28 Mar 2008 14:05:32 -0500 20, 34 -- Received: from localhost (localhost [[UNIX: localhost]]) 20, 34 -- by messagescreen2.ars.usda.gov (8.13.8/8.13.8/Submit) with SMTP id m2SJ2Cwc010507 20, 34 -- for {microscopy-at-microscopy.com} ; Fri, 28 Mar 2008 14:02:12 -0500 20, 34 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 20, 34 -- Fri, 28 Mar 2008 13:00:19 -0600 20, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 34 -- Content-class: urn:content-classes:message 20, 34 -- MIME-Version: 1.0 20, 34 -- Content-Type: text/plain; 20, 34 -- charset="US-ASCII" 20, 34 -- Subject: RE: [Microscopy] coloring epon 20, 34 -- Date: Fri, 28 Mar 2008 13:00:19 -0600 20, 34 -- Message-ID: {8017F94146BF634DA9414E4B9088525B01054107-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 20, 34 -- In-Reply-To: {200803281825.m2SIPtDE014535-at-ns.microscopy.com} 20, 34 -- X-MS-Has-Attach: 20, 34 -- X-MS-TNEF-Correlator: 20, 34 -- Thread-Topic: [Microscopy] coloring epon 20, 34 -- thread-index: AciRASrghoYIOHeaQf66HhG+Rq/eWAACZs0g 20, 34 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 20, 34 -- To: {Elliott-at-Arizona.edu} , {microscopy-at-microscopy.com} 20, 34 -- X-OriginalArrivalTime: 28 Mar 2008 19:00:19.0734 (UTC) FILETIME=[F7BDE360:01C89105] 20, 34 -- X-MessageScreenMessageID: 1206730932.899835.1248.103874609 20, 34 -- X-MessageScreenContentScore: Score of 0 assigned to Content 20, 34 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 20, 34 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 20, 34 -- Content-Transfer-Encoding: 8bit 20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2SJ5YMm020566 ==============================End of - Headers==============================
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Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Cooperative Oxford Laboratory, NOAA
Title-Subject: [Filtered] Knife Maker
Question: I purchased the new Leica knife maker at the same time I purchased the UC6. I have been using both successfuly for four years. I highly recommend both. Good luck in your purchase.
I realize that I was unclear in my question. I am not cutting the epon face (in which case circling the biology would help), but I am cutting cross-sections. Thus when I am facing my block, I am looking down at the edge of the biology. I am trying to remove as much of the excess epon as possible without losing the biology.
Were I to write on the block face I would risk damaging the biology right at the air/epon interface.
David
On Mar 28, 2008, at 3:39 PM, Ralph Common wrote: } I use a histology pen that is highly resistant to solvents to circle } the } area of interest before the second embedding. It helps a lot. } } Ralph Common } Michigan State University } } -----Original Message----- } From: Elliott-at-Arizona.edu [mailto:Elliott-at-Arizona.edu] } Sent: Friday, March 28, 2008 1:22 PM } To: rcommon-at-msu.edu } Subject: [Microscopy] coloring epon } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } I am flat embedding some very thin biological samples (they are grown } on aclar film and embedded). Normally it is very easy to find the } sample, it is on the edge of the plastic. } } For some of what I am doing, after I remove the aclar film I put more } epon in its place and incubate it again. This works very well to } protect the biology that is right at the air/epon interface. The } difficulty is that it can be VERY hard to find the biology. } } I was wondering if I would color the epon (we are using Embed-812 - } EM Science (#14120)) so that it would be easy to find the line } between the colored and uncolored epon? } } Thank you } David } } _________________________ } } } David Elliott Ph.D. } Assistant Professor - Department of Cell Biology and Anatomy } Director, Research Microscopy Core Service } University of Arizona College of Medicine } PO Box 245004 } Tucson, AZ 85724 } } Voice: 520-626-7870 } Fax: 520-626-2097 } } ==============================Original } Headers============================== } 8, 20 -- From Elliott-at-Arizona.edu Fri Mar 28 13:15:43 2008 } 8, 20 -- Received: from smtpgate.email.arizona.edu } (gandalf.email.Arizona.EDU [128.196.133.169]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m2SIFhoV003909 } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } 13:15:43 -0500 } 8, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu } [10.0.0.204]) } 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 494E7554F4F } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } 11:15:43 -0700 (MST) } 8, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } 0B555554F4C } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } 11:15:43 -0700 (MST) } 8, 20 -- Mime-Version: 1.0 (Apple Message framework v753) } 8, 20 -- Content-Transfer-Encoding: 7bit } 8, 20 -- Message-Id: {29BD405C-88B3-415F-B8B4- } A3A3CB5563DB-at-Arizona.edu} } 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } 8, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 8, 20 -- From: David Elliott {Elliott-at-Arizona.edu} } 8, 20 -- Subject: coloring epon } 8, 20 -- Date: Fri, 28 Mar 2008 11:15:41 -0700 } 8, 20 -- X-Mailer: Apple Mail (2.753) } 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 7, 23 -- From Elliott-at-Arizona.edu Fri Mar 28 20:09:12 2008 7, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2T19Bpj001069 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 20:09:11 -0500 7, 23 -- Received: from gandalfs_amavis (amavis2.email.arizona.edu [10.0.0.205]) 7, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 78380557689 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 18:09:11 -0700 (MST) 7, 23 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 7, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id D6ADA5576B6 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 18:09:09 -0700 (MST) 7, 23 -- Message-Id: {F695DF22-04B3-497B-8775-250FC313989A-at-Arizona.edu} 7, 23 -- From: David Elliott {Elliott-at-Arizona.edu} 7, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 7, 23 -- In-Reply-To: {002701c89124$96273060$7d7a0923-at-msu.edu} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) 7, 23 -- Subject: Re: [Microscopy] coloring epon 7, 23 -- X-Priority: 3 (Normal) 7, 23 -- Date: Fri, 28 Mar 2008 18:09:08 -0700 7, 23 -- References: {002701c89124$96273060$7d7a0923-at-msu.edu} 7, 23 -- X-Mailer: Apple Mail (2.919.2) 7, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (Kathrine.Scholtz-at-students.wits.ac.za) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, March 15, 2008 at 12:15:07 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both Kathrine.Scholtz-at-students.wits.ac.za as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: Kathrine.Scholtz-at-students.wits.ac.za Name: Kate Scholtz
Organization: University of the Witwatersrand
Education: Graduate College
Location: Johannesburg, South Africa
Title: Scanning electron microscopy
Question: Hi I am a graduate student from South Africa currently visiting a laboratory at Harvard University. I am currently attempting to view Vascular Smooth Muscle cells and cardiac myocytes using a Scanning Electron Microscope. I am fixing the cells with 3% Gluteraldehyde/3% Paraformaldehyde in 0.2M Sodium Cacodylate buffer, 1% Osmium Tetroxide and dehydrating in a series of graded ethanol into 100%. Following dehydration I am processing the cells in HMDS in varying concentrations (1:3; 1:1 and 3:1) in 100% ethanol and then leaving it in an incubator at 37C overnight to dehydrate. I am using HMDS as CPD resulted in large surface cracks and generally very ppor preservation. While the HMDS appears to work better I am still not getting good results. The cell membranes of all the cells are being pulled off exposing the inner cell structures. Does anyone know of a good way to keep the cell membranes intact? Or maybe suggestions on what I might be doing wrong to cause the ripping off of the cell membrane?
I think that something is not right with the CPD processing; there is no reason that cells or muscle should have large cracks after CPD if done correctly. You may want to review the protocol and read the instructions very carefully and review the procedure with someone there who is very familiar with the equipment. There is some chance that the equipment, if automated, is not functioning correctly or that there is a misunderstanding about the process steps.
Especially check the following:
* The final changes of ethanol (or amyl acetate, etc.) before going into the CPD must be absolutely free of water.
* The sample must stay covered at all times by fluid: do not allow the sample to "drain" and become uncovered by fluid at any point in the exchange of ethanol to liquid CO2. The Balzers CPD030 has instructions on the case to "drain and refill several times" but this should never leave the sample drained, always use multiple partial drain/fill cycles to remove the original solvent.
* Depending on the chamber geometry and amount of liquid CO2 that you start with when beginning the heating phase, the sample could possibly "go dry" before the critical point is attained. Follow the instructions carefully; they usually state the starting level of liquid CO2. If too much the pressure can become too high and blow out the protective burst-disc.
* Agitate the sample (depends on your system how this would be done) to promote exchange and full elimination of the ethanol (etc.). You should not smell ethanol (etc) when the final exchanges are made, nor when you open the chamber at the end.
* Heat slowly in the heating phase. This is typically automatic, but should take ~15 min or more to reach the final temp (~38C).
* Vent very slowly - should take another 10-15 min. to come back to room temperature. This is often manual, so make sure you leak it slowly.
* Once out and properly dried, you must keep it dry; minimize handling in the atmosphere, especially if humid conditions.
* Be careful that glue or conductive paint solvents do not wick into the very dry and porous sample during mounting, again wetting it.
If you would send a copy of the CPD protocol it would be helpful for assessing possible problems. Again, there is no reason that you should have large surface cracks in this sample.
Kathrine.Scholtz-at-students.wits.ac.za wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by } (Kathrine.Scholtz-at-students.wits.ac.za) } from } http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html } on Saturday, March 15, 2008 at 12:15:07 } Remember to consider the Grade/Age of the student when considering the Question } --------------------------------------------------------------------------- } Please reply to both Kathrine.Scholtz-at-students.wits.ac.za as well } as to the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: Kathrine.Scholtz-at-students.wits.ac.za } Name: Kate Scholtz } } Organization: University of the Witwatersrand } } Education: Graduate College } } Location: Johannesburg, South Africa } } Title: Scanning electron microscopy } } Question: Hi } I am a graduate student from South Africa currently visiting a } laboratory at Harvard University. I am currently attempting to view } Vascular Smooth Muscle cells and cardiac myocytes using a Scanning } Electron Microscope. I am fixing the cells with 3% Gluteraldehyde/3% } Paraformaldehyde in 0.2M Sodium Cacodylate buffer, 1% Osmium } Tetroxide and dehydrating in a series of graded ethanol into 100%. } Following dehydration I am processing the cells in HMDS in varying } concentrations (1:3; 1:1 and 3:1) in 100% ethanol and then leaving it } in an incubator at 37C overnight to dehydrate. I am using HMDS as CPD } resulted in large surface cracks and generally very ppor } preservation. While the HMDS appears to work better I am still not } getting good results. The cell membranes of all the cells are being } pulled off exposing the inner cell structures. Does anyone know of a } good way to keep the cell membranes intact? Or maybe suggestions on } what I might be doing wrong to cause the ripping off of the cell } membrane? } } Thank you in advance } } Kind Regards } Kate Scholtz } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 12 -- From zaluzec-at-microscopy.com Sat Mar 29 07:02:50 2008 } 10, 12 -- Received: from [192.168.1.107] (msdvpn8.msd.anl.gov [130.202.238.72]) } 10, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2TC2mHj006494 } 10, 12 -- for {microscopy-at-microscopy.com} ; Sat, 29 Mar 2008 07:02:49 -0500 } 10, 12 -- Mime-Version: 1.0 } 10, 12 -- Message-Id: {p06240806c413e04dc214-at-[192.168.1.107]} } 10, 12 -- Date: Sat, 29 Mar 2008 07:02:52 -0500 } 10, 12 -- To: microscopy-at-microscopy.com } 10, 12 -- From: Kathrine.Scholtz-at-students.wits.ac.za (by way of Ask-A-Microscopist) } 10, 12 -- Subject: AskAMicroscopist: Does anyone know of a good way to keep the cell } 10, 12 -- membranes intact? } 10, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 22 -- From dac-at-research.umass.edu Sat Mar 29 08:51:54 2008 17, 22 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2TDpsMw022386 17, 22 -- for {microscopy-at-microscopy.com} ; Sat, 29 Mar 2008 08:51:54 -0500 17, 22 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 17, 22 -- (authenticated bits=0) 17, 22 -- by race4.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m2TDpg16000495 17, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 17, 22 -- Sat, 29 Mar 2008 09:51:43 -0400 17, 22 -- Message-ID: {47EE5788.8050008-at-research.umass.edu} 17, 22 -- Date: Sat, 29 Mar 2008 09:51:52 -0500 17, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 17, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.12) Gecko/20080201 SeaMonkey/1.1.8 17, 22 -- MIME-Version: 1.0 17, 22 -- To: Kathrine.Scholtz-at-students.wits.ac.za, 17, 22 -- Microscopy List {microscopy-at-microscopy.com} 17, 22 -- Subject: Re: [Microscopy] AskAMicroscopist: Does anyone know of a good way 17, 22 -- to keep the cell 17, 22 -- References: {200803291208.m2TC8qiA015019-at-ns.microscopy.com} 17, 22 -- In-Reply-To: {200803291208.m2TC8qiA015019-at-ns.microscopy.com} 17, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I'm surprised at the interest generated by this equipment, thanks to everyone who replied especially to Mr. Morris and his enthusiasm
A bit of background which I left out in my original post, the microscopes are in Sudbury Ontario Canada, fairly remote from most people with interest, shipping these will cost a bit. The lab here supports mining exploration, thus the microscopes and accessories are set-up for petrographic/mineralogical work.
I will look into getting some value for the microscopes failing that I will contact those of you who were looking for a donation
Regards,
Ben
-----Original Message----- X-from: Keith J Morris [mailto:kjmorris-at-well.ox.ac.uk] Sent: March 27, 2008 5:50 AM To: Vandenberg, Ben (Sudbury) Cc: Microscopy-at-microscopy.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ben.vandenberg-at-valeinco.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ben.vandenberg-at-valeinco.com Name: Ben Vanden Berg
Title-Subject: [Filtered] LM Equipment, Zeiss, Standard Universal and Ultraphot II
Question: I have 3 microscopes which have been collecting dust for years and I would like to sell or donate
Does anyone know the value of the following items and the best place to auction equipment like this?
All microscopes are compound models, Zeiss, 1960ís vintage
1 standard universal, polarizing, transmitted light equipment for reflected light 1 standard universal, polarizing, transmitted light, florescence and photography equipped 1 ultraphot II, monster photography machine numerous accessories
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
We invite you to submit an abstract for the 9th Asia-pacific Microscopy Conference (APMC9). APMC is a new name of APEM (formerly known as Asia-Pacific Electron Microscopy). APMC9 will be held in Jeju international convention center, Korea on November 2-7, 2008.
The conference has a long history of 52 years focusing on the materials science, medical science, biological science, and instrumentation and analytical techniques of microscopy. In addition to the scientific sessions of oral and poster presentation, photographic competition, social events and tours are programmed. APMC9 reserved funding for scholarship as well.
Please visit www.apmc9.or.kr for the information of the APMC9.
We look forward to your participation and seeing you in beautiful resort island, Jeju, in Korea.
Young-Woon Kim Associate Professor Department of Materials Science and Engineering Seoul National University Tel) +82-2-880-7977 Fax) +82-2-883-8197 E-mail) APMC9-at-plaza.snu.ac.kr
==============================Original Headers============================== 13, 28 -- From youngwk-at-snu.ac.kr Mon Mar 31 23:35:54 2008 13, 28 -- Received: from nabi1.snu.ac.kr (nabi1.snu.ac.kr [147.46.10.149]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m314ZqiV000572 13, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 31 Mar 2008 23:35:52 -0500 13, 28 -- Received: from [147.46.10.146] ([147.46.10.146]) 13, 28 -- by nabi1.snu.ac.kr ([147.46.10.149]) 13, 28 -- with ESMTP id 2008040113:35:52:391136.581.3024419744 13, 28 -- for {Microscopy-at-microscopy.com} ; 13, 28 -- Tue, 01 Apr 2008 13:35:52 +0900 (KST) 13, 28 -- Received: from [147.46.233.110] ([147.46.233.110]) 13, 28 -- by auk2.snu.ac.kr ([147.46.10.146]) 13, 28 -- with ESMTP id 2008040113:35:50:487671.32404.92744624 13, 28 -- for {Microscopy-at-microscopy.com} ; 13, 28 -- Tue, 01 Apr 2008 13:35:50 +0900 (KST) 13, 28 -- From: "Young-Woon Kim" {youngwk-at-snu.ac.kr} 13, 28 -- To: {Microscopy-at-microscopy.com} 13, 28 -- Subject: APMC9 call for papers - deadline extended to 4/30 13, 28 -- Date: Tue, 1 Apr 2008 13:34:57 +0900 13, 28 -- Message-ID: {014401c893b1$bd41a6f0$37c4f4d0$-at-ac.kr} 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Type: text/plain; 13, 28 -- charset="us-ascii" 13, 28 -- Content-Transfer-Encoding: 7bit 13, 28 -- X-Mailer: Microsoft Office Outlook 12.0 13, 28 -- Thread-Index: AciTsb0P6YB/POwVTdGJk1yBrJJJqw== 13, 28 -- Content-Language: ko 13, 28 -- X-TERRACE-SPAMMARK: NO (SR:15.62) 13, 28 -- (by Terrace) ==============================End of - Headers==============================
1. What is the proper way to sterilze a water dipping lens for use in an aseptic environment?
2. What should be the heating rate to minimize thermal shock to a microscope objective lens when bringing it in and out of the incubation chamber e.g., between 37C and 20C?
Thanks.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
I have found out a little bit more about this option on the CM100 after talking ot FEI. I hope this info can be useful for other users of the Philips CM100 TEM.
The serial connection kit is simply a null modem cable. This option needs to be enabled in the key attached to the System Definition Board and a few jumpers set on the SDB and the single board computer. The remote software for the CM series is rather limited in only uploading and downloading alignment data and will not be useful to monitor other operating parameters e.g., the ones relevant for system shutdowns that I am experiencing.
Reseating the boards and a cold start with RAM init solved the problem with the wehnelt S.W. protection and I can now actually turn on the high tension and use the scope. But the scope is still shutting down every 4-5 days. One thing I do know is that the circulating water is fine, there is no overheating. FEI will come and do a check up to see what can be wrong. If anybody is interested, I can report back when the problem is solved. Cheers.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
On Sat, 23 Feb 2008, wpchan-at-u.washington.edu wrote:
} Can anyone tell me what are contained in the Serial connection kit } (PW6443/00) and the Remote control function (PW6460/00)? } } We have a Philips CM100 TEM and I am trying to figure out how to get the } remote control function to work. I found the CM Monitor and Remote } control software (PW6470/40, rcm.sys driver and cmonitor.exe) but not } anything on the Serial connection kit (PW6443/00), and the Remote control } function (PW6460/00). } } I am hoping to use the software to monitor the machine because it keeps } shutting itself down after 2-3 days. All air and water flow seems to be } fine, and vacuum appears to be normal except P2 is rather high (~78). } The other major problem is that the wehnelt S.W. protection on the high } tension buffer board is tripped right from startup and cannot be rectified } by pushing the reset button; so high tension cannot be switch on. } } I plan to call FEI next week but just hope that someone on the list is } familiar with the cm100 to shed some light on the remote monitor function } and the wehnelt S.W. protection reset. Thanks a lot.
Hello, I have some comments on monitoring Philips CM100 scope via Remote function: Is it true, that communication between CM100 and PC goes through NullModem cable. However, one can monitor also other data from CM100, not only the alignment. There is a nice package of CM Remote Utilities written by Max Otten. It's 16bit windows application, but it can run on Win2k, WinNT, Win3.1, Win98 (There are some troubles with WinXP). This package contains an utility CMLIST.exe. By this utility you can read and monitor following data from CM100:
Nearly all the data you need to know about the microscope and its state in time course.
We use this utility when we are in troubles with our CM100 (not so frequently ;-)).
If you need more details, please let me know.
Best regards from Prague Oldrich
On 1 Apr 2008 at 15:55, wpchan-at-u.washington.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have found out a little bit more about this option on the CM100 after } talking ot FEI. I hope this info can be useful for other users of the } Philips CM100 TEM. } } The serial connection kit is simply a null modem cable. This option needs } to be enabled in the key attached to the System Definition Board and a few } jumpers set on the SDB and the single board computer. The remote software } for the CM series is rather limited in only uploading and downloading } alignment data and will not be useful to monitor other operating } parameters e.g., the ones relevant for system shutdowns that I am } experiencing. } } Reseating the boards and a cold start with RAM init solved the problem } with the wehnelt S.W. protection and I can now actually turn on the high } tension and use the scope. But the scope is still shutting down every 4-5 } days. One thing I do know is that the circulating water is fine, there is } no overheating. FEI will come and do a check up to see what can be wrong. } If anybody is interested, I can report back when the problem is solved. } Cheers. } } -- } Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) } The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/) } } On Sat, 23 Feb 2008, wpchan-at-u.washington.edu wrote: } } } Can anyone tell me what are contained in the Serial connection kit } } (PW6443/00) and the Remote control function (PW6460/00)? } } } } We have a Philips CM100 TEM and I am trying to figure out how to get the } } remote control function to work. I found the CM Monitor and Remote } } control software (PW6470/40, rcm.sys driver and cmonitor.exe) but not } } anything on the Serial connection kit (PW6443/00), and the Remote control } } function (PW6460/00). } } } } I am hoping to use the software to monitor the machine because it keeps } } shutting itself down after 2-3 days. All air and water flow seems to be } } fine, and vacuum appears to be normal except P2 is rather high (~78). } } The other major problem is that the wehnelt S.W. protection on the high } } tension buffer board is tripped right from startup and cannot be rectified } } by pushing the reset button; so high tension cannot be switch on. } } } } I plan to call FEI next week but just hope that someone on the list is } } familiar with the cm100 to shed some light on the remote monitor function } } and the wehnelt S.W. protection reset. Thanks a lot. } } ==============================Original Headers============================== } 6, 22 -- From wpchan-at-u.washington.edu Tue Apr 1 15:52:35 2008 } 6, 22 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m31KqZLh005123 } 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2008 15:52:35 -0500 } 6, 22 -- Received: from homer22.u.washington.edu (homer22.u.washington.edu [140.142.15.9]) } 6, 22 -- by mxout5.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m31KqYTa024055 } 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2008 13:52:35 -0700 } 6, 22 -- Received: from localhost (wpchan-at-localhost) } 6, 22 -- by homer22.u.washington.edu (8.13.7+UW06.06/8.13.7+Submit) with ESMTP id m31KqYq3009154 } 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 1 Apr 2008 13:52:34 -0700 } 6, 22 -- Date: Tue, 1 Apr 2008 13:52:34 -0700 (PDT) } 6, 22 -- From: "W. Chan" {wpchan-at-u.washington.edu} } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- Subject: Re: [Microscopy] cm100 remote control } 6, 22 -- In-Reply-To: {200802232130.m1NLUVa0027787-at-ns.microscopy.com} } 6, 22 -- Message-ID: {Pine.LNX.4.64.0804011308030.24409-at-homer22.u.washington.edu} } 6, 22 -- References: {200802232130.m1NLUVa0027787-at-ns.microscopy.com} } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } 6, 22 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.4.1.134231 } 6, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' } ==============================End of - Headers==============================
------------------------------------------ Oldrich Benada Mikrobiologický ústav AV CR Videnska 1083 142 20 Praha 4 - Krc
==============================Original Headers============================== 12, 25 -- From benada-at-biomed.cas.cz Wed Apr 2 01:50:42 2008 12, 25 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m326odAu010194 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Apr 2008 01:50:42 -0500 12, 25 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 12, 25 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 7FFFC1A44417; 12, 25 -- Wed, 2 Apr 2008 08:50:38 +0200 (CEST) 12, 25 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 12, 25 -- Organization: Institute of Microbiology 12, 25 -- To: wpchan-at-u.washington.edu 12, 25 -- Date: Wed, 02 Apr 2008 08:50:39 +0200 12, 25 -- MIME-Version: 1.0 12, 25 -- Subject: Re: [Microscopy] Re: cm100 remote control 12, 25 -- CC: microscopy-at-microscopy.com 12, 25 -- Message-ID: {47F348DF.4995.2C3590-at-benada.biomed.cas.cz} 12, 25 -- Priority: normal 12, 25 -- In-reply-to: {200804012055.m31KtMSD009073-at-ns.microscopy.com} 12, 25 -- References: {200804012055.m31KtMSD009073-at-ns.microscopy.com} 12, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 25 -- Content-type: text/plain; charset=ISO-8859-2 12, 25 -- Content-description: Mail message body 12, 25 -- X-Antivirus: avast! (VPS 080401-0, 01.04.2008), Outbound message 12, 25 -- X-Antivirus-Status: Clean 12, 25 -- Content-Transfer-Encoding: 8bit 12, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id m326odAu010194 ==============================End of - Headers==============================
We have three CM microscopes with the current commercial version of the remote control software running on computers with XP Pro. I have been pleased with the software and have been able to write a couple of small programs (Visual C++) that help us track diagnostics. One simply polls the microscope to see if the HT is on and writes a log file when it shuts off (typically during the night or over a weekend.) This has been useful for diagnostics. I found that the VB tutorial they include is a few versions back and causes some issues, but the Delphi tutor runs just fine and can read most of the microscope info.
The problem presented by the original poster reminds me of some of the problems we have diagnosed. When the microscope shuts down does it give a diagnostic message or does the computer go completely down? The high value of P2 does not concern me if it comes down when the buffer tank is pumped (you can trigger this by pressing the vacuum ON button.) When there is a problem with the buffer being pumped, one often gets a CBP (critical backing pressure) error message because the diff pump sees a pressure rise. That typically does not cause a full shutdown of the microscope, but just the vacuum system.
Have you had your building electrician monitor the power to the microscope over time? Our building managers have a habit of shutting down services at night and turning them on in the early morning. The voltage can drift significantly with the load. You may find that the load on the line has changed and the voltage drifts out of spec. This can often be remedied by changing the tpas used on the isolation transformer. This is best done under the guidance of a service engineer.
Wish you well. Trying to keep older microscopes running can be really frustrating.
==============================Original Headers============================== 4, 19 -- From jrminter-at-rochester.rr.com Wed Apr 2 06:22:12 2008 4, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m32BMBaN002501 4, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Apr 2008 06:22:11 -0500 4, 19 -- Received: from hrndva-web20-z02 ([10.128.132.171]) 4, 19 -- by hrndva-smta02.mail.rr.com with ESMTP 4, 19 -- id {20080402112210.OUAS7675.hrndva-smta02.mail.rr.com-at-hrndva-web20-z02} 4, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Apr 2008 11:22:10 +0000 4, 19 -- Message-ID: {25808196.434531207135330978.JavaMail.root-at-hrndva-web20-z02} 4, 19 -- Date: Wed, 2 Apr 2008 7:22:10 -0400 4, 19 -- From: {jrminter-at-rochester.rr.com} 4, 19 -- To: Microscopy-at-microscopy.com 4, 19 -- Subject: Re: [Microscopy] cm100 remote control 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; charset=utf-8 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- X-Priority: 3 (Normal) 4, 19 -- Sensitivity: Normal 4, 19 -- X-Originating-IP: from 69.207.155.112 by webmail.rochester.rr.com; Wed, 2 Apr 2008 11:22:10 +0000 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Research Fellowship in Electron Microscopy
Question: Research Fellowship in Electron Microscopy 2008 Monash University, Melbourne, Australia. ______________________________________
The Faculty of Science and the Monash Centre for Electron Microscopy (MCEM) have established a Research Fellowship in Electron Microscopy to attract an outstanding researcher to conduct an independent program of research of major importance in the area of electron microscopy in the physical sciences.
The Fellow will be provided with a salary, a contribution to research expenses, a travel grant and access to the electron microscopy facilities in the MCEM. The MCEM has a suite of instrumentation, including a double-aberration corrected FEI Titan 80-300 FEG-S/TEM, a JEOL 2100F FEG-S/TEM and a JEOL 7001F FEG-SEM, all of which are located in a purpose built, ultrastable building designed to optimise instrument performance (see www.mcem.monash.edu.au for details).
Salary Range: Appointment will be at either Academic Level A or Level B, depending upon qualifications and experience. ($48, 896 - $66, 360 p.a) Academic Level A plus generous superannuation ($69, 853 - $82, 951 p.a) Academic Level B plus generous superannuation
Duration: Three ñyear appointment
Enquiries: A/Prof Joanne Etheridge, Director, MCEM on (+613) 9905 1836 or email: joanne.etheridge-at-mcem.monash.edu.au
To Apply: Applicants must read the document ìConditions of Fellowship in Electron Microscopyî and fill out the ìApplication Form for Fellowship in Electron Microscopyî which are available at: http://www.mcem.monash.edu.au/news-activities/research-fellowship-08.html.
The completed application form must be submitted as a signed PDF via email to: Madeleine.Einsiedel-at-mcem.monash.edu.au by 30 April 2008.
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Email: camiller-at-anatomy.iupui.edu Name: Caroline Miller
Organization: Indiana Microscopy Society
Title-Subject: [Filtered] Meeting Announcement
Question: The Indiana Microscopy Society is holding their annual Spring Meeting on the campus of Indiana University, Bloomington on April 18th. Check out the INMS website, www.indianamicroscopy.org for more details.
When the scope shuts down, the computer goes dead and only the 24 V standby power remains on. I have to turn off the power completely, wait a few hours, and then I can turn the machine back on. We did have the + and - 15 V power supplies failed recently, and the 24 V power supply sometimes does not come on right away. So the problem could be related to these powersupplies.
I have never checked the incoming power and may be I should ask our Physical Plant people to take a look. Thanks for the advice.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
On Wed, 2 Apr 2008, jrminter-at-rochester.rr.com wrote:
} We have three CM microscopes with the current commercial version of the } remote control software running on computers with XP Pro. I have been } pleased with the software and have been able to write a couple of small } programs (Visual C++) that help us track diagnostics. One simply polls } the microscope to see if the HT is on and writes a log file when it } shuts off (typically during the night or over a weekend.) This has been } useful for diagnostics. I found that the VB tutorial they include is a } few versions back and causes some issues, but the Delphi tutor runs just } fine and can read most of the microscope info. } } The problem presented by the original poster reminds me of some of the } problems we have diagnosed. When the microscope shuts down does it give } a diagnostic message or does the computer go completely down? The high } value of P2 does not concern me if it comes down when the buffer tank is } pumped (you can trigger this by pressing the vacuum ON button.) When } there is a problem with the buffer being pumped, one often gets a CBP } (critical backing pressure) error message because the diff pump sees a } pressure rise. That typically does not cause a full shutdown of the } microscope, but just the vacuum system. } } Have you had your building electrician monitor the power to the } microscope over time? Our building managers have a habit of shutting } down services at night and turning them on in the early morning. The } voltage can drift significantly with the load. You may find that the } load on the line has changed and the voltage drifts out of spec. This } can often be remedied by changing the tpas used on the isolation } transformer. This is best done under the guidance of a service engineer. } } Wish you well. Trying to keep older microscopes running can be really frustrating.
I am observing 110 nm thick sections of Epon-embedded biological material at 80 kV by TEM (copper grids). The material was: osmium and en-bloc uranyl contrasted, then lead citrate and uranyl acetate constrasted. Then I perform EDX analysis of parts of these sections. Obviously, at low mag, I get the regular peaks for the expected elements present: Cu, Pb, U, Os. At higher magnification, I see only the peaks for Pb and Cu, I don't see the U and Os peaks probably because they are too weak, it is possible and it is all right. But strangely I get a pretty high signal at 2,610 keV, which I attributed to Cl. This peak is higher than the 2,340 keV peak of Pb, which tells me that it is not due to Pb. It is not Ag too. It may be Rn but I really wonder how I could detect so much Rn in a deep vacuum! I wondered if people who do EDX on biological sections also see a Cl peak. I would be surprised it is due to the salt present in the tissue, not only because the technique is probable not sensitive enough to detect it, but also because in this case I should also see a Na peak. Please note that I took a background signal in Epon without biological material and I can only detect Cu.
Any idea someone?
Stephane
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Email: mbisher-at-princeton.edu Name: Margaret Bisher
Question: I have someone here who would like me to try to fix their cells with potassium permanganate as the primary fixative.
I know that this was done many years ago. That is about all I can find when I do a search - that it was used before aldehydes became the standard fixative of choice.
Does anyone have some sort of protocol that I could try? I will be working with tissue culture cells and really interested in looking at cell membranes, which is why we want to try it.
Thanks for any help you might be able to pass my way.
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Email: meng.wang-at-monsanto.com Name: Megan
Title-Subject: [Filtered] Immunohistochemistry vs. Western Blot
Question: Dear Fellow Scientists,
I'm wondering what is the current consensus on Western blot and immunohistochemistry. Does a good journal "requires" both a correct band in Western blot AND immunolocalization data for publication? If your protein does not show up in Western but can be detected with immunohistochemistry, would null mutant and a competition assay sufficient to support what is seen with immunohistochemistry is real?
My second somewhat related question is: is Western blot considered more sensitive than immunohistocheistry? My understanding is that it depends on the type of protein you are working with and the expression pattern. For example, if the protein of interest is expressed only in a very small population of cells, Western may end up diluting the protein.
It would be good to hear what other scientists have in mind.
Permangenate was used as a plant cell fixative --look up papers by Mollenhauer from the 1960's, and also by J.D. Robertson in his studies of membrane structure --those papers are from the early 1960's. The original paper was by Luft, J.H.. J. Biophys. Biochem. Cytology (the precursor to J. Cell Bio) 2:799-801 (1956) You can probably get some insight from issues of the Journal of Cell Biology at that time.
I also have a copy of a book by Clinton Dawes from 1988 that compiles many of the techniques. The book was published by Ladd, the EM supply company, and was called "Introduction to Biological Electron Microscopy". I used it for many years when I taught an EM course here.
Best of Luck!
Date sent: Thu, 3 Apr 2008 08:45:32 -0500 To: jbs-at-temple.edu X-from: mbisher-at-princeton.edu Send reply to: mbisher-at-princeton.edu
Hi Meng, There is no simple answer to your question. Many papers show immunocytochemistry and no westerns (please note, we capitalize Southern to recognize Professor Southern who invented the DNA blot; but the protein blot was not invented by a Professor Western, so western blot must not be capitalized). They do this when well characterized antibodies are used. However, if you have made an antibody, I doubt you could publish immunocytochem without supporting western blots. The western is needed to help show that the ab is specific. On the other hand, I doubt you be obliged to show immunocytochemistry. If you have nice westerns and a bunch of ip's with a new antibody, then that would probably be fine. Of course, there may be reasons why doing localization would support/confirm your conclusions but I don't think such work is required to demonstrate the usefulness of an ab.
Hope this helps, Tobias Baskin } } } Email: meng.wang-at-monsanto.com } Name: Megan } } Title-Subject: [Filtered] Immunohistochemistry vs. Western Blot } } Question: Dear Fellow Scientists, } } I'm wondering what is the current consensus on Western blot and } immunohistochemistry. Does a good journal "requires" both a correct } band in Western blot AND immunolocalization data for publication? If } your protein does not show up in Western but can be detected with } immunohistochemistry, would null mutant and a competition assay } sufficient to support what is seen with immunohistochemistry is real? } } My second somewhat related question is: is Western blot considered } more sensitive than immunohistocheistry? My understanding is that it } depends on the type of protein you are working with and the } expression pattern. For example, if the protein of interest is } expressed only in a very small population of cells, Western may end } up diluting the protein. } } It would be good to hear what other scientists have in mind. } } Thanks much, } } Megan }
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I need a JOEL OM-40 vacuum optical microscope that had the hole in the prism to transmit the electron beam. These were used to place samples right on the Rowland Circle of the WDS systems of 840 and 6400 microprobes. I have another application involving focused MeV ions. I would happily play for such a used microscope if it is in good condition. The manual would be nice as well.
Barney L. Doyle, Manager Sandia National Laboratories Radiation Solid Interactions Dept. 1111 PO Box 5800, MS 1056 Albuquerque, NM, 87185 Phone: 1 505 844 7568 Fax: 1 505 844 7775
==============================Original Headers============================== 5, 33 -- From bldoyle-at-sandia.gov Thu Apr 3 18:38:30 2008 5, 33 -- Received: from sentry.sandia.gov (mm03snlnto.sandia.gov [132.175.109.20]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m33NcTpI031013 5, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Apr 2008 18:38:29 -0500 5, 33 -- Received: from [134.253.165.159] by sentry.sandia.gov with ESMTP (SMTP 5, 33 -- Relay 01 (Email Firewall v6.3.1)); Thu, 03 Apr 2008 17:38:15 -0600 5, 33 -- X-Server-Uuid: AA8306FD-23D1-4E5B-B133-B2D9F10C3631 5, 33 -- Received: from ES04SNLNT.srn.sandia.gov ([134.253.165.154]) by 5, 33 -- Cas1.srn.sandia.gov ([134.253.165.159]) with mapi; Thu, 3 Apr 2008 5, 33 -- 17:38:15 -0600 5, 33 -- From: "Doyle, Barney L" {bldoyle-at-sandia.gov} 5, 33 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 5, 33 -- Date: Thu, 3 Apr 2008 17:38:14 -0600 5, 33 -- Subject: Need JOEL OM-40, will buy 5, 33 -- Thread-Topic: Need JOEL OM-40, will buy 5, 33 -- Thread-Index: AciRB7xhfqLC531wS12nogZrpJC0VwE2+X8Q 5, 33 -- Message-ID: {A21F951765B778488C2FF4E3AF836A5F202C0DEA55-at-ES04SNLNT.srn.sandia.gov} 5, 33 -- Accept-Language: en-US 5, 33 -- Content-Language: en-US 5, 33 -- X-MS-Has-Attach: 5, 33 -- X-MS-TNEF-Correlator: 5, 33 -- acceptlanguage: en-US 5, 33 -- MIME-Version: 1.0 5, 33 -- X-TMWD-Spam-Summary: TS=20080403233816; SEV=2.2.2; DFV=B2008040318; 5, 33 -- IFV=2.0.4,4.0-9; AIF=B2008040318; RPD=5.02.0125; ENG=IBF; 5, 33 -- RPDID=7374723D303030312E30413031303230312E34374635364136372E303034342C73733D312C6667733D30; 5, 33 -- CAT=NONE; CON=NONE 5, 33 -- X-MMS-Spam-Filter-ID: B2008040318_5.02.0125_4.0-9 5, 33 -- X-WSS-ID: 6BEBB5ED2GK3507091-01-01 5, 33 -- Content-Type: text/plain; 5, 33 -- charset=us-ascii 5, 33 -- Content-Transfer-Encoding: 8bit 5, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m33NcTpI031013 ==============================End of - Headers==============================
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Email: judi.medlin-at-jax.org Name: Judi Medlin
Organization: The Jackson Laboratory
Title-Subject: [Filtered] Short Course on Frontiers in Microscopy: Whole Animal Imaging
Question: Greetings from Maine! The Jackson Laboratory is offering a short course this summer on Whole Animal Imaging. Details are posted online at http://courses.jax.org/2008/microscopy_08.html
We are currently accepting applications, the application form may be downloaded from the website.
Any assistance you may be able to provide by bringing this to the attention of your listserv members would be greatly appreciated! Have a wonderful day, Judi Medlin Conference Specialist p: 207-288-6326
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Question: Seeking full time Lab Technician to prepare samples in asebstos microscopy laboratory. Will train the right person. What is the right person? Degree in sciences and microscopy preparation experience preferred but not necessary, attention to detail, must be productive and quality oriented. Immediate need. Laboratory located in Kennesaw, GA.
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On May 2, an impressive line-up of speakers will discuss analysis at the atomic scale at the Advanced Materials/ Failure Analysis (AMFA) Workshop. Discounted registration is available until April 11.
This one-day workshop occurs immediately post the IRPS conference held at the same location. The registration fee is nominal, and the 2008 program continues the AMFA tradition of new information presented by leading-edge researchers. This is an opportunity you won't want to miss. ------------------------------------------------------------------------ --------------------------------------
The 2008 AMFA Workshop is financially sponsored by Omniprobe, Inc., FEI Company, and Gatan. Technical co-sponsors include the IEEE Reliability Society and the Electronic Device Failure Analysis Society. Members of the societies receive a registration discount.
PROGRAM This year's exciting single day program consists of invited speakers covering a variety of topics emphasizing analysis at the atomic scale in a series of 40 minute presentations, each followed by 20 minutes of facilitator-led audience discussion.
David Joy - Oak Ridge National Labs & University of Tennessee "Is There a Future for the SEM?"
Phillip Russell - Appalachian State University "Nanoscale Applications of FIB Systems"
Alan Street - Qualcomm "Failure Analysis in an IFM Environment"
Martino Poggio - IBM / Stanford "Ultrasensitive Force Detection Applied to Nuclear Magnetic Resonance"
Brian Gorman - University of North Texas "3-D Atomic Scale Characterization of Semiconductors Using Atom Probe Tomography"
Rod Ruoff - University of Texas "In-situ Nano-manipulation for New Science"
REGISTRATION Online registration is open: {http://www.amfaworkshop.org/registration.html}
Standard Fees: $250.00 for IEEE or EDFAS members, $300.00 for non-members. $50 Early Bird Discount expires APRIL 11, 2008.
Registration for the AMFA workshop provides the following: - A full day of talks on a variety of emerging and high-impact subjects from speakers invited on the basis of exceptional new content and presentation expertise. - Participation in break-out discussions. - Two breaks and a buffet lunch. - A CD containing the full set of presentations.
Online registration closes at 5pm on MAY 1, 2008. On-site registration will be available on MAY 2, 2008 from 7am to noon by the Phoenix Ballroom, Hyatt Regency Hotel.
} It is very strange that you don't see the Cl peak from just } Epon since most Epons do contain chlorine - at least those used for } routine embedding of biological specimens. Do you not see the Cl at } low mags? All sorts of other issues could be in play - one needs to } know the exact conditions - beam current (beam damage, mass } loss/gain?), probe time, side entry or high angle x-ray detector? } (take-off angle), tilted or horizontal specimen. Are you probing } very close to a grid bar (absorption by the Cu?), are there any } apertures in the way (you need to remove the objective aperture), } are you in Scanning or TEM mode? etc.
PI
} } Dear Listers, } } I am observing 110 nm thick sections of Epon-embedded biological } material at 80 kV by TEM (copper grids). } The material was: osmium and en-bloc uranyl contrasted, then lead } citrate and uranyl acetate constrasted. } Then I perform EDX analysis of parts of these sections. Obviously, } at low mag, I get the regular peaks for the expected elements } present: Cu, Pb, U, Os. } At higher magnification, I see only the peaks for Pb and Cu, I don't } see the U and Os peaks probably because they are too weak, it is } possible and it is all right. } But strangely I get a pretty high signal at 2,610 keV, which I } attributed to Cl. This peak is higher than the 2,340 keV peak of Pb, } which tells me that it is not due to Pb. It is not Ag too. It may be } Rn but I really wonder how I could detect so much Rn in a deep } vacuum! } I wondered if people who do EDX on biological sections also see a Cl } peak. I would be surprised it is due to the salt present in the } tissue, not only because the technique is probable not sensitive } enough to detect it, but also because in this case I should also see } a Na peak. } Please note that I took a background signal in Epon without } biological material and I can only detect Cu. } } Any idea someone? } } Stephane } } } ______________________________________________________________________________
-- Peter Ingram Adj. Professor of Pathology, Duke University Medical Center Box 90319 LaSalle Street Extension DURHAM NC USA 27708-0319
I received, as usual, lots of helpful messages to my question and I want to thank all of you who answered. All critics go in the same direction: Cl peak should come from Epon. I was skeptical, since I compared a spectrum in the biological material with a spectrum in Epon alone next to the biological material and there I could find no Cl peak. Of course I compared both in the same conditions, same mag, same kV, same beam size, without obj. aperture, and so on. One person said that I should consider the fact that Cl could be evaporated in the column due to the beam energy.
Taking these remarks in consideration, I collected several EDX spectra in areas with Epon alone and found a Cl peak! I don’t know why sometimes I find no peak for Cl, but the most probable cause is that it has been evaporated by the beam. I tested multiple spectra collections of the same area and it confirms this hypothesis: the Cl peak decreases at each further data collection!
Thanks again for your help!
Stephane
____________________________________________________________________________________ You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. http://tc.deals.yahoo.com/tc/blockbuster/text5.com
==============================Original Headers============================== 7, 21 -- From nizets2-at-yahoo.com Fri Apr 4 03:38:00 2008 7, 21 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m348bx7o012643 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 4 Apr 2008 03:37:59 -0500 7, 21 -- Received: (qmail 22976 invoked by uid 60001); 4 Apr 2008 08:37:59 -0000 7, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 21 -- s=s1024; d=yahoo.com; 7, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 21 -- b=fI1OexPvDeL4M5mO51pHBMW73NrqeZ+1iTuAOSK7FsbS7OVViiHutZ9Po2UPq0bx+ewC6DSdKkhGroEp+SLQq/3OEB7dBY85iiHK+HEG7NrljbabdBlIsasMUd7qaXDEyCS2VNFtdNLRV/OzHL98lu27SNEzZQrshrYIiNB5xJU=; 7, 21 -- X-YMail-OSG: nma7nSkVM1k1BOpikbV7USBcbeXwrJZ728I3f9TawqGsFrNN25bWtqsjDWPp_bq90MtsXcqT7y62qNm6OdJG9LWFjjqG6Ojb7R2iCMPdRBrMuwFaCps- 7, 21 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Fri, 04 Apr 2008 01:37:59 PDT 7, 21 -- X-Mailer: YahooMailRC/902.40 YahooMailWebService/0.7.185 7, 21 -- Date: Fri, 4 Apr 2008 01:37:59 -0700 (PDT) 7, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 21 -- Subject: Re: [Microscopy] Chlorine, EDX and TEM - SOLVED 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=utf-8 7, 21 -- Message-ID: {534080.22763.qm-at-web37408.mail.mud.yahoo.com} 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m348bx7o012643 ==============================End of - Headers==============================
For the past few years the Annual Microscopy & Microanalysis meeting has hosted a session for the children who travel with us to the meeting. This session received the title "It's a Family Affair" as a last minute name idea back for Honolulu - and it stuck.
So the organisers are wondering and looking for a perhaps better name?
With all the creative minds on the list - any suggestions?
Thanks!
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 8, 22 -- From edelmare-at-muohio.edu Fri Apr 4 07:15:35 2008 8, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m34CFZp9002330 8, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Apr 2008 07:15:35 -0500 8, 22 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 8, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m34CFZHF018096 8, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Apr 2008 08:15:35 -0400 8, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 8, 22 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m34CFY9p014316 8, 22 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Apr 2008 08:15:34 -0400 8, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 8, 22 -- To: microscopy-at-Microscopy.com 8, 22 -- Date: Fri, 04 Apr 2008 08:15:36 -0400 8, 22 -- MIME-Version: 1.0 8, 22 -- Subject: M&M Meeting Question 8, 22 -- Message-ID: {47F5E3A8.11970.86A599E-at-edelmare.muohio.edu} 8, 22 -- Priority: normal 8, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 8, 22 -- Content-type: text/plain; charset=US-ASCII 8, 22 -- Content-transfer-encoding: 7BIT 8, 22 -- Content-description: Mail message body 8, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
As I had these days to take some metallurgical pictures on the optical microscope for some students, I have fight (and lost), to have an acceptable white balance setting of the digital camera, in DIC mode.
I made the settings in bright field, where it was ok, but switch to DIC, the colors were far from what I see in the occulars. Light turqoise blue become marine blue and so on. All colors are much too saturated. Playing in DIC with the white balance gave nothing as one has nowhere somthing white. And the color setings arn't valid in live mode, only on captured pictures.
Has someone an advice to get a correct white balance in DIC mode (reflected light) or cross polar (transimited light). The camera is JenOptik Progress C10+ and the control software is the ProCap-2.1. But I suppose it's the same way, wathever the camera brand.
Thanks in advance
Jacques F.
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
You are trying to indicate the interface, correct? A light Sputter coating of the initial interface would work nicely eh? And will not harm the biology and would be very visable in the TEM or LM.
On 28 Mar 2008 at 21:10, Elliott-at-Arizona.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } I realize that I was unclear in my question. I am not cutting the } epon face (in which case circling the biology would help), but I am } cutting cross-sections. Thus when I am facing my block, I am looking } down at the edge of the biology. I am trying to remove as much of the } excess epon as possible without losing the biology. } } Were I to write on the block face I would risk damaging the biology } right at the air/epon interface. } } David } } } On Mar 28, 2008, at 3:39 PM, Ralph Common wrote: } } I use a histology pen that is highly resistant to solvents to circle } } the } } area of interest before the second embedding. It helps a lot. } } } } Ralph Common } } Michigan State University } } } } -----Original Message----- } } From: Elliott-at-Arizona.edu [mailto:Elliott-at-Arizona.edu] } } Sent: Friday, March 28, 2008 1:22 PM } } To: rcommon-at-msu.edu } } Subject: [Microscopy] coloring epon } } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi } } I am flat embedding some very thin biological samples (they are grown } } on aclar film and embedded). Normally it is very easy to find the } } sample, it is on the edge of the plastic. } } } } For some of what I am doing, after I remove the aclar film I put more } } epon in its place and incubate it again. This works very well to } } protect the biology that is right at the air/epon interface. The } } difficulty is that it can be VERY hard to find the biology. } } } } I was wondering if I would color the epon (we are using Embed-812 - } } EM Science (#14120)) so that it would be easy to find the line } } between the colored and uncolored epon? } } } } Thank you } } David } } } } _________________________ } } } } } } David Elliott Ph.D. } } Assistant Professor - Department of Cell Biology and Anatomy } } Director, Research Microscopy Core Service } } University of Arizona College of Medicine } } PO Box 245004 } } Tucson, AZ 85724 } } } } Voice: 520-626-7870 } } Fax: 520-626-2097 } } } } ==============================Original } } Headers============================== } } 8, 20 -- From Elliott-at-Arizona.edu Fri Mar 28 13:15:43 2008 } } 8, 20 -- Received: from smtpgate.email.arizona.edu } } (gandalf.email.Arizona.EDU [128.196.133.169]) } } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } m2SIFhoV003909 } } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } } 13:15:43 -0500 } } 8, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu } } [10.0.0.204]) } } 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } } 494E7554F4F } } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } } 11:15:43 -0700 (MST) } } 8, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } } 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } } 0B555554F4C } } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } } 11:15:43 -0700 (MST) } } 8, 20 -- Mime-Version: 1.0 (Apple Message framework v753) } } 8, 20 -- Content-Transfer-Encoding: 7bit } } 8, 20 -- Message-Id: {29BD405C-88B3-415F-B8B4- } } A3A3CB5563DB-at-Arizona.edu} } } 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } } 8, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } } 8, 20 -- From: David Elliott {Elliott-at-Arizona.edu} } } 8, 20 -- Subject: coloring epon } } 8, 20 -- Date: Fri, 28 Mar 2008 11:15:41 -0700 } } 8, 20 -- X-Mailer: Apple Mail (2.753) } } 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original Headers============================== } 7, 23 -- From Elliott-at-Arizona.edu Fri Mar 28 20:09:12 2008 } 7, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2T19Bpj001069 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 20:09:11 -0500 } 7, 23 -- Received: from gandalfs_amavis (amavis2.email.arizona.edu [10.0.0.205]) } 7, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 78380557689 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 18:09:11 -0700 (MST) } 7, 23 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) } 7, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id D6ADA5576B6 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 18:09:09 -0700 (MST) } 7, 23 -- Message-Id: {F695DF22-04B3-497B-8775-250FC313989A-at-Arizona.edu} } 7, 23 -- From: David Elliott {Elliott-at-Arizona.edu} } 7, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 7, 23 -- In-Reply-To: {002701c89124$96273060$7d7a0923-at-msu.edu} } 7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 7, 23 -- Content-Transfer-Encoding: 7bit } 7, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) } 7, 23 -- Subject: Re: [Microscopy] coloring epon } 7, 23 -- X-Priority: 3 (Normal) } 7, 23 -- Date: Fri, 28 Mar 2008 18:09:08 -0700 } 7, 23 -- References: {002701c89124$96273060$7d7a0923-at-msu.edu} } 7, 23 -- X-Mailer: Apple Mail (2.919.2) } 7, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Apr 4 12:02:20 2008 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m34H2KbX008619 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Apr 2008 12:02:20 -0500 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m34H2IQO027732; 10, 25 -- Fri, 4 Apr 2008 13:02:18 -0400 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m34H2HJs023301; 10, 25 -- Fri, 4 Apr 2008 13:02:18 -0400 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: "Elliott-at-Arizona.edu" {Elliott-at-Arizona.edu} 10, 25 -- Date: Fri, 04 Apr 2008 13:02:17 -0400 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] Re: coloring epon 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {47F626D9.21672.970CBE0-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200803290110.m2T1AAOX002216-at-ns.microscopy.com} 10, 25 -- References: {200803290110.m2T1AAOX002216-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Alternatives (which had slipped my mind), since I used to play with left over resin as a student.
These are for coloring the unpolymerized "Topping" layer.
1) Carbon particles from a carbon rod sharpener work very well and settle on to the interface. (You could try pencil dust or hewlett- packard laser jet toner. I know HP toner does not disolve in resin but not all others do)
2) Using older accelerator results in very yellow/orange resins and may work we for you.
3) A number of light microscopy "stain crytals" mix nicely into unpolymerized resin and result in weird colors: Fast Green, Crystal violet, etc. They are dry some should not result in polymerization or sectioning problems (but to be honest I only used them for paper weights and desktop play things, I never sectioned them). Oh, yes the colors are not the "Normal" colors I assume due to the fact that they are not in an aqueous solution.
Good luck.
On 28 Mar 2008 at 21:10, Elliott-at-Arizona.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } I realize that I was unclear in my question. I am not cutting the } epon face (in which case circling the biology would help), but I am } cutting cross-sections. Thus when I am facing my block, I am looking } down at the edge of the biology. I am trying to remove as much of the } excess epon as possible without losing the biology. } } Were I to write on the block face I would risk damaging the biology } right at the air/epon interface. } } David } } } On Mar 28, 2008, at 3:39 PM, Ralph Common wrote: } } I use a histology pen that is highly resistant to solvents to circle } } the } } area of interest before the second embedding. It helps a lot. } } } } Ralph Common } } Michigan State University } } } } -----Original Message----- } } From: Elliott-at-Arizona.edu [mailto:Elliott-at-Arizona.edu] } } Sent: Friday, March 28, 2008 1:22 PM } } To: rcommon-at-msu.edu } } Subject: [Microscopy] coloring epon } } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi } } I am flat embedding some very thin biological samples (they are grown } } on aclar film and embedded). Normally it is very easy to find the } } sample, it is on the edge of the plastic. } } } } For some of what I am doing, after I remove the aclar film I put more } } epon in its place and incubate it again. This works very well to } } protect the biology that is right at the air/epon interface. The } } difficulty is that it can be VERY hard to find the biology. } } } } I was wondering if I would color the epon (we are using Embed-812 - } } EM Science (#14120)) so that it would be easy to find the line } } between the colored and uncolored epon? } } } } Thank you } } David } } } } _________________________ } } } } } } David Elliott Ph.D. } } Assistant Professor - Department of Cell Biology and Anatomy } } Director, Research Microscopy Core Service } } University of Arizona College of Medicine } } PO Box 245004 } } Tucson, AZ 85724 } } } } Voice: 520-626-7870 } } Fax: 520-626-2097 } } } } ==============================Original } } Headers============================== } } 8, 20 -- From Elliott-at-Arizona.edu Fri Mar 28 13:15:43 2008 } } 8, 20 -- Received: from smtpgate.email.arizona.edu } } (gandalf.email.Arizona.EDU [128.196.133.169]) } } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } m2SIFhoV003909 } } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } } 13:15:43 -0500 } } 8, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu } } [10.0.0.204]) } } 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } } 494E7554F4F } } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } } 11:15:43 -0700 (MST) } } 8, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) } } 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id } } 0B555554F4C } } 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 } } 11:15:43 -0700 (MST) } } 8, 20 -- Mime-Version: 1.0 (Apple Message framework v753) } } 8, 20 -- Content-Transfer-Encoding: 7bit } } 8, 20 -- Message-Id: {29BD405C-88B3-415F-B8B4- } } A3A3CB5563DB-at-Arizona.edu} } } 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed } } 8, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } } 8, 20 -- From: David Elliott {Elliott-at-Arizona.edu} } } 8, 20 -- Subject: coloring epon } } 8, 20 -- Date: Fri, 28 Mar 2008 11:15:41 -0700 } } 8, 20 -- X-Mailer: Apple Mail (2.753) } } 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } } ==============================End of - } } Headers============================== } } } } } } } ==============================Original Headers============================== } 7, 23 -- From Elliott-at-Arizona.edu Fri Mar 28 20:09:12 2008 } 7, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) } 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2T19Bpj001069 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 20:09:11 -0500 } 7, 23 -- Received: from gandalfs_amavis (amavis2.email.arizona.edu [10.0.0.205]) } 7, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 78380557689 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 18:09:11 -0700 (MST) } 7, 23 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) } 7, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id D6ADA5576B6 } 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 28 Mar 2008 18:09:09 -0700 (MST) } 7, 23 -- Message-Id: {F695DF22-04B3-497B-8775-250FC313989A-at-Arizona.edu} } 7, 23 -- From: David Elliott {Elliott-at-Arizona.edu} } 7, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} } 7, 23 -- In-Reply-To: {002701c89124$96273060$7d7a0923-at-msu.edu} } 7, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 7, 23 -- Content-Transfer-Encoding: 7bit } 7, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) } 7, 23 -- Subject: Re: [Microscopy] coloring epon } 7, 23 -- X-Priority: 3 (Normal) } 7, 23 -- Date: Fri, 28 Mar 2008 18:09:08 -0700 } 7, 23 -- References: {002701c89124$96273060$7d7a0923-at-msu.edu} } 7, 23 -- X-Mailer: Apple Mail (2.919.2) } 7, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 15, 25 -- From edelmare-at-muohio.edu Fri Apr 4 12:21:03 2008 15, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m34HL3mv021920 15, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Apr 2008 12:21:03 -0500 15, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 15, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m34HL1hN011185; 15, 25 -- Fri, 4 Apr 2008 13:21:01 -0400 15, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 15, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m34HL15A028112; 15, 25 -- Fri, 4 Apr 2008 13:21:01 -0400 15, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 15, 25 -- To: "Elliott-at-Arizona.edu" {Elliott-at-Arizona.edu} 15, 25 -- Date: Fri, 04 Apr 2008 13:21:00 -0400 15, 25 -- MIME-Version: 1.0 15, 25 -- Subject: Re: [Microscopy] Re: coloring epon 15, 25 -- CC: microscopy-at-Microscopy.com 15, 25 -- Message-ID: {47F62B3C.30902.981EE93-at-edelmare.muohio.edu} 15, 25 -- Priority: normal 15, 25 -- In-reply-to: {200803290110.m2T1AAOX002216-at-ns.microscopy.com} 15, 25 -- References: {200803290110.m2T1AAOX002216-at-ns.microscopy.com} 15, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 15, 25 -- Content-type: text/plain; charset=US-ASCII 15, 25 -- Content-transfer-encoding: 7BIT 15, 25 -- Content-description: Mail message body 15, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Dear Members. I have some samples that I think are labeled with an antibody conjugated to a gold particle. The samples were labeled using a preembedding method. I wonder if there is any chemical reaction that generates a product visible under the light microscope that can be used on thick resin sections to detect the presence of gold particles. Thanks!!!!
Jaime Llodra Graduate Student NYU
==============================Original Headers============================== 2, 23 -- From jal490-at-nyu.edu Fri Apr 4 12:35:43 2008 2, 23 -- Received: from mx1.nyu.edu (MX1.NYU.EDU [128.122.108.241]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m34HZgxx002709 2, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 4 Apr 2008 12:35:43 -0500 2, 23 -- Received: from mail.nyu.edu (F3.HOME.NYU.EDU [128.122.108.93]) 2, 23 -- by mx1.nyu.edu (8.13.8/8.13.8) with ESMTP id m34HZg0h018136 2, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 4 Apr 2008 13:35:42 -0400 (EDT) 2, 23 -- Received: from [216.165.126.103] by mail.alt.home.nyu.edu (mshttpd); Fri, 2, 23 -- 04 Apr 2008 13:35:42 -0400 2, 23 -- From: Jaime A Llodra {jal490-at-nyu.edu} 2, 23 -- To: Microscopy-at-Microscopy.Com 2, 23 -- Message-ID: {f20f95c2807.47f62eae-at-mail.nyu.edu} 2, 23 -- Date: Fri, 04 Apr 2008 13:35:42 -0400 2, 23 -- X-Mailer: Sun Java(tm) System Messenger Express 6.3-5.02 (built Oct 12 2, 23 -- 2007; 32bit) 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Language: en 2, 23 -- Subject: Detection of nanogold particles by some color development? 2, 23 -- X-Accept-Language: en 2, 23 -- Priority: normal 2, 23 -- Content-Type: text/plain; charset=us-ascii 2, 23 -- Content-Disposition: inline 2, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both krschier-at-mtu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Both MSA and MAS offer Student Awards (PSA & DSA respectively) In addition there are Student Bursaries all of whihc are to help students attend the meeting. You are too late to apply for the PSA/DSA , but you can still apply for the Student Bursary.
Look at the meeting WWW page
http://mm2008.microscopy.org
Follow the navigation link called Awards/Support scroll down the whole page to look over all the possible awards and their details. Some are for technical staff but the majority are for students.
Nestor Your Friendly Neighborhood SysOp
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear All, there are 20 more seats available thanks to the generosity of the Ettore Majorana Center that will be assigned on a FIFO rule (first in first out), for
INTERNATIONAL SCHOOL OF BIOPHYSICS «ANTONIO BORSELLINO» 36th Course: “MULTIDIMENSIONAL OPTICAL FLUORESCENCE MICROSCOPY TOWARDS NANOSCOPY”, that will be held in ERICE, SICILY, ITALY on 19- 29 APRIL 2008. Prof. A. ZICHICHI is the PRESIDENT OF CCSEM AND DIRECTOR OF THE CENTRE, and Prof. A. DIASPRO (UNIVERSITY OF GENOA) and Prof. V.TORRE (SISSA, TRIESTE), are DIRECTORS OF THE COURSE (http://www.ccsem.infn.it/). Download poster in the News at www.lambs.it.
PURPOSE OF THE COURSE A bright new future has appeared, as the nano-era has taken and placed a whole new array of tools in the hands of biophysicists, who are keen to go deeper into the intricacies of how biological systems work. Forever pushing the boundaries, biophysicists are sliding the research focus from the micro- towards the nano- and even sub-nano scale. Now, Biophysics is a molecular science, rapidly moving to the nanoscale, demanding for an interdisciplinary approach more than in the past, as in the Antonio Borsellino’s expectations. It seeks to explain biological function in terms of the molecular structures and properties of specific molecules. As part of this effort, some biophysicists are involved in inventing new methods and building new instruments for monitoring these structures. Many of the exciting new developments in microscopy and more specifically in optical microscopy, in terms of imaging and manipulation, spectroscopy and visualization, are a segment and necessity of this trend. The recent advances to the “nano” level, both as complement to electron and scanning probe microscopy and as development of the so-called optical nanoscopy, witnesses the relevance of the field.
TOPICS and LECTURERS In vivo imaging, SHG *; P. BIANCHINI, University of Genoa, IT Fluorescence Spectroscopy, GFP Photophysics *; R. BIZZARRI, NESTINFM, SNS, Pisa, IT; Optics, Confocal Microscopy, THG *; F. BRAKENHOFF, University of Amsterdam, NL; FRAP, Single particle tracking *; K. BRAECKMANS, Ghent University, BE; Single molecule force spectroscopy *; J. BRUJIC, New York University, USA – EBSA lecturer; Fluctuation Microscopies for biological tissues G. CHIRICO, University of Milan-Bicocca, IT; Micro-particle manipulation *; D. COJOC, TASC, INFM, Trieste, IT; SHG, CARS, 2PE *; C. COMBS, NIH, Bethesda, USA; 2PE, 3D imaging *; A. DIASPRO, University of Genoa,IT – EBSA President Elect, BJ EBM; Micro/Nano Optical Manipulation *; E. Di FABRIZIO, University of Catanzaro Magna Graecia, IT; Raster Image Correlation Spectroscopy, Photon Counting *; M. DIGMAN, UC Irvine, USA; High-content screening *; M. FARETTA, IFOM-IEO, Milan, IT; Correlative Microscopy *; U. FASCIO, University of Milan, IT; Fluorescence Lifetime, FRET *; H.C. GERRITSEN, Utrecht University, NL; FCS, Global Data Analysis *; E. GRATTON, UC Irvine, USA – BJ EBM; Time lapse imaging *; S. GUIDO, University of Naples, IT; Fluorescente Optical Nanoscopy *; S. HELL, MPI, Goettingen, DE; Scanning Microscopy, Optical aberrations *; M. MARTINEZ CORRAL, Univ. of Valencia, ES; Optical systems, Scanning Microscopy *; F. QUERCIOLI, CNR-ISC, Florence, IT; 2PE imaging in vivo *; GIMMI RATTO, Institute of Neuroscience, CNR, Pisa, IT; 2PE, Fast scanning methods *; P. SAGGAU, Baylor College of Med. Houston, Texas,USA; Correlative Microscopy at cryo-Temperatures *; A. SARTORI, Institut Pasteur, Paris, FR; Light Scattering, FCS applications *; P.L. SAN BIAGIO, CNR-IBF, Palermo, IT – SIBPA President; Molecular landscapes by means of AFM *; G. SCOLES, Princeton University, USA; Linear and Non linear Optical Microscopy *; C. SHEPPARD, Ntl Univ. of Singapore, Singapore; Laser Light Sheet Fluorescence Microscopy *; P. KELLER, EMBL, Heidelberg, DE; Laser scissors and tweezers in cell biology *; I. TOLIC-NORRELYKKE, MPI, Dresden, DE – SIBPA Lecturer; Fluorescence imaging in Neuroscience *; V. TORRE, SISSA, Trieste, IT; Quantitative colocalization *; C. USAI, CNR-IBF, Genoa, IT; Confocal Microscopy, Structured light methods *; T. WILSON, University of Oxford, UK; Photoswitch-activatable fluorescent proteins, Lifetime *; F.WOUTERS, Univ. of Goettingen, DE.
PRACTICAL INFORMATION
Deadline: April 15th, 2008 Send an e-mail, asap, to: diaspro-at-fisica.unige.it
Cost: 1000 Euros, it includes scientific material, lodging and meals for the whole period (can be paid on site or by bank transfer - ask for coordinates) and transportation from/to the Palermo/Trapani airport. Participants are supposed to arrive in Erice on April 19th, possibly not later than 5 p.m. Please, provide asap your travel details. The course starts on April 20th 8:30 a.m. and ends on April 28th , 2008, 8:00 p.m.
Lessons scheduling, the Program is built on the topics reported in this message: 8,30-9,15; 9,30-10,15; break; 10,45-11,30; 11,45-12,30; LUNCH; 14,45-15,15 Companies and Students time; 15,30-16,15; 16,30-17,15; break; 17,45-18,30; 18,45-19,30; 21-23 Free time in the Canteen. There will be time for Students presentations (10-20 minutes slots) There will be the possibility of submitting an article for the Springer related book.
There are 20 more seats available thanks to the generosity of the Ettore Majorana Center that will be assigned on a FIFO rule (first in first out)
ALL the BEST ALBY
---------------------------------------------------- "Water slowly flowed under the sky" (Cesare Pavese) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 21, 24 -- From sekkio-at-mac.com Sun Apr 6 01:46:07 2008 21, 24 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com [17.148.16.78]) 21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m366k7cF002984 21, 24 -- for {microscopy-at-microscopy.com} ; Sun, 6 Apr 2008 01:46:07 -0500 21, 24 -- Received: from mac.com (asmtp008-s [10.150.69.71]) 21, 24 -- by smtpoutm.mac.com (Xserve/smtpout015/MantshX 4.0) with ESMTP id m366vvPm024231; 21, 24 -- Sat, 5 Apr 2008 23:57:57 -0700 (PDT) 21, 24 -- Received: from [10.0.1.2] (host118-121-dynamic.21-79-r.retail.telecomitalia.it [79.21.121.118]) 21, 24 -- (authenticated bits=0) 21, 24 -- by mac.com (Xserve/asmtp008/MantshX 4.0) with ESMTP id m366vpmP017228 21, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 21, 24 -- Sat, 5 Apr 2008 23:57:53 -0700 (PDT) 21, 24 -- Message-Id: {B7C1942F-467D-497D-A29F-DE6B49FF6563-at-mac.com} 21, 24 -- From: Diaspro {sekkio-at-mac.com} 21, 24 -- To: microscopy-at-microscopy.com, MicroscopyListServer by {venu-at-purdue.edu} , 21, 24 -- Confocal List Microscopy {CONFOCAL-at-LISTSERV.BUFFALO.EDU} , 21, 24 -- mplsm-users-at-yahoogroups.com 21, 24 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 21, 24 -- Mime-Version: 1.0 (Apple Message framework v919.2) 21, 24 -- Subject: Last minute Seats - School on Multidimensional Microscopy, Erice Sicily 19-29 April 2008 21, 24 -- Date: Sun, 6 Apr 2008 08:57:50 +0200 21, 24 -- X-Mailer: Apple Mail (2.919.2) 21, 24 -- Content-Transfer-Encoding: 8bit 21, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m366k7cF002984 ==============================End of - Headers==============================
What's the most practicable solvent for cleaning old (but not burnt) Santovac from a diff pump?
cheers
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 7, 26 -- From r.sims-at-auckland.ac.nz Sun Apr 6 20:21:25 2008 7, 26 -- Received: from mailhost.auckland.ac.nz (moe.its.auckland.ac.nz [130.216.12.35]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m371LOj6004724 7, 26 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Apr 2008 20:21:25 -0500 7, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id EEC2A480584 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Apr 2008 13:21:22 +1200 (NZST) 7, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 7, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 7, 26 -- by localhost (moe.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 7, 26 -- with ESMTP id RDefoyK900uy for {Microscopy-at-microscopy.com} ; 7, 26 -- Mon, 7 Apr 2008 13:21:22 +1200 (NZST) 7, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 7, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id CCF2B480563 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Apr 2008 13:21:22 +1200 (NZST) 7, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Date: Mon, 07 Apr 2008 13:24:17 +1200 7, 26 -- MIME-Version: 1.0 7, 26 -- Subject: Cleanup Santovac? 7, 26 -- Message-ID: {47FA2081.22714.144A3AA-at-r.sims.auckland.ac.nz} 7, 26 -- Priority: normal 7, 26 -- X-mailer: Pegasus Mail for Windows (4.41) 7, 26 -- Content-type: text/plain; charset=US-ASCII 7, 26 -- Content-transfer-encoding: 7BIT 7, 26 -- Content-description: Mail message body ==============================End of - Headers==============================
I remembered this, but couldn't put my finge on it at the time. I ran across it by accident and figured I'd send it.
Talbot, et al.: Image analysis of insulation mineral fibres, J Microsc, 200, 3, 251-268, 2000.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
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-----Original Message----- X-from: yuhong.wu-at-solvay.com [mailto:yuhong.wu-at-solvay.com] Sent: Friday, March 21, 2008 9:37 AM To: ph2-at-sprynet.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying
please copy both yuhong.wu-at-solvay.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I wonder if there is a good image analysis tool or method that can measure glass/carbon fiber (those found in polymers as fillers) length automatically. Typically we need 300+ data points/sample for statistical analysis. We use a semi-auto method and only the data log-in portion is automatic.
My understanding is that overlapping of fibers (which is unavoidable) makes automated measurements difficult. I'd like to ask for your suggestion/advice. Thank you very much!
Disclaimer: Ladd Research has used and sold Santovac, Solvon B, Acetone, Vacuum Evaporators, Diff Pumps and other Microscopy supplies for over 50 years
At 09:28 PM 4/6/2008, r.sims-at-auckland.ac.nz wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Listers, I have a client who wants to immuno-gold label some fungus infected plant material. I have read some papers on the subject but want more advice. Please contact me off list with questions and advice.
Thanks for all your help, Jeannette
-- Jeannette Taylor, Technologist II Integrated Electron Microscopy Core Cherry L. Emerson Hall, Room E106 Emory University 1515 Dickey Drive Atlanta, Georgia 30322
There are lots of sources of colorants for epoxies; the main problem is finding a dye (preferably not a pigment) that mixes perfectly with epoxy resin and leaves the cured material crystal-clear, even under magnification. I searched for "epoxy dye" using Google and got many hits. The first one happened to be www.eagerplastics.com/7701.htm, which took me to a company that could probably consult on a good colorant. I know nothing about this company; and there are numerous others. So choose at your own risk.
Petrographers often need a colored polymer embedment. A common choice is a blue-dyed epoxy, which is sold by some petrography or microscopy supply houses. An interesting table is available on-line which gives as a footnote some info on epoxy dye suppliers: www.petrography.net/Table%20-%20Castable%20resins%20and%20dyes.pdf
-------Roy Arrowood (915)747-6934 direct (915)747-5468 secretaries arrowood-at-utep.edu M-201L Engineering Science Complex University of Texas at El Paso
==============================Original Headers============================== 5, 20 -- From arrowood-at-utep.edu Mon Apr 7 13:47:09 2008 5, 20 -- Received: from itdsrvmail07.utep.edu (itdsrvmail07.utep.edu [129.108.0.197]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m37Il76j000974 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Apr 2008 13:47:09 -0500 5, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 20 -- Content-class: urn:content-classes:message 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="us-ascii" 5, 20 -- Subject: dyes for epoxy 5, 20 -- Date: Mon, 7 Apr 2008 12:47:06 -0600 5, 20 -- Message-ID: {02AF78229B459A4383FF97E542A9FA2D0AC1E0-at-itdsrvmail07.utep.edu} 5, 20 -- X-MS-Has-Attach: 5, 20 -- X-MS-TNEF-Correlator: 5, 20 -- Thread-Topic: dyes for epoxy 5, 20 -- Thread-Index: AciY38cuPL86NtilRkO4tV1LVOdGzA== 5, 20 -- From: "Arrowood, Roy" {arrowood-at-utep.edu} 5, 20 -- To: {Microscopy-at-microscopy.com} 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m37Il76j000974 ==============================End of - Headers==============================
The Santovac diffusion pump fluid is a polyphenyl ether compound with a molecular weight of about 455. As such, it is both highly viscous and rather insoluble. (see p.183-4 of Vacuum Methods in Electron Microscopy). However, since it is based on a molecular structure consisting of benzene rings (i.e. the phenyl groups) it will tend to be most soluble in solvents that also contain benzene rings, such as toluene and xylene. Acetone and methyl-ethyl-ketone are also helpful solvents, and mixtures of these with toluene and xylene are sometimes recommended. The usual procedure is to wipe off as much of the fluid as possible with dry rags or tissues, then follow up by wiping with pads moistened with one of these solvents.
For parts that can withstand such mistreatment I have also found that washing with hot water and liberal amounts of one of the modern detergents that are formulated for degreasing automobile engines can be helpful.
Finally, rinse with isopropyl alcohol. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
Bazett-Jones (http://www.sickkids.ca/bazett-joneslab/default.asp) has done some work with Quantum Dots using an energy filtered TEM. See: Nisman R, Dellaire G, Ren Y, Li R, Bazett-Jones DP.
Application of quantum dots as probes for correlative fluorescence, conventional, and energy-filtered transmission electron microscopy. J Histochem Cytochem. 2004 Jan;52(1):13-8. PMID: 14688213 [PubMed - indexed for MEDLINE]
Wadowska-at-upei.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } I am looking for reference papers on quantum dots application for electron microscopy. Has anybody used QD for EM? } TIA } Dorota } } } } ==============================Original Headers============================== } 3, 21 -- From Wadowska-at-grpwise.novell.upei.ca Fri Mar 28 08:05:28 2008 } 3, 21 -- Received: from mx2.upei.ca (magellanic.cs.upei.ca [137.149.3.22]) } 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m2SD5RpC032704 } 3, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2008 08:05:27 -0500 } 3, 21 -- Received: from [137.149.64.174] (helo=grpwise.novell.upei.ca) } 3, 21 -- by mx2.upei.ca with esmtp (Exim 4.50 #1 (Debian)) } 3, 21 -- id 1JfEGs-00048q-SC } 3, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Mar 2008 10:05:26 -0300 } 3, 21 -- Received: from gwiadom-MTA by grpwise.novell.upei.ca } 3, 21 -- with Novell_GroupWise; Fri, 28 Mar 2008 10:05:26 -0300 } 3, 21 -- Message-Id: {47ECC2CD.EA7E.0081.0-at-groupwise.upei.ca} } 3, 21 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP } 3, 21 -- Date: Fri, 28 Mar 2008 10:05:00 -0300 } 3, 21 -- From: "Dorota Wadowska" {Wadowska-at-upei.ca} } 3, 21 -- To: {Microscopy-at-microscopy.com} } 3, 21 -- Subject: EM quantum dots } 3, 21 -- Mime-Version: 1.0 } 3, 21 -- Content-Type: text/plain; charset=US-ASCII } 3, 21 -- Content-Disposition: inline } 3, 21 -- Content-Transfer-Encoding: 8bit } 3, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m2SD5RpC032704 } ==============================End of - Headers============================== }
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
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Email: mlibbee-at-gmail.com Name: Marissa Libbee
Title-Subject: [Filtered] SEM HV Tank
Question: Hello Listers,
Alas, the seven year old SEM I use is down for repair and the component that contributed to the maintenance issue is the HV tank. My expertise in anything HV-tank related is close to nil and I am wondering if anyone has an accessible literature recommendation that may improve my understanding of the anatomy of the tank. I welcome any email descriptions as well.
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Email: mbisher-at-princeton.edu Name: Peggy Bisher
Question: I just ordered and received a vacuum tissue processor for paraffin embedding. I was wondering if any of you could share with me your recipes for processing.
Up until now the students have been processing their samples manually for paraffin embedding.
I have quite a few good reference books that I have been that I have looked through but I thought it would be good to ask the following questions:
What sort of times and concentrations of fixative, ethanol, zylene, etc. do you have your machine set up for when you put your tissue in to process?
One of the books I read goes from formalin right into 95% ethanol and that just sounds wrong to me. Coming from the TEM background, I would never consider starting at 95%, I am a bit concerned about that. I just was wondering what some of you might d.
I am hoping you might be able to let me know what your recipe is for standard tissue processing. Over here most of the students work with small tissue samples, drosphila and even c. elegans. On occasion, there might be bone tissue, but that is rare.
Any suggestions/ good starting points would be most helpful.
Hi listers, I vaguely remember there is a contral on 2010F to adjust the frequency and amplitude when aligning the HT center. But I haven't used it for years, and cannot recall the knob or command. Does anyone know it, or it does not exist at all?
Thanks
Shu Miao
==============================Original Headers============================== 3, 20 -- From s.miao-at-sheffield.ac.uk Tue Apr 8 20:20:02 2008 3, 20 -- Received: from stoat.shef.ac.uk (stoat.shef.ac.uk [143.167.2.187]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m391K1x9016429 3, 20 -- for {Microscopy-at-Microscopy.Com} ; Tue, 8 Apr 2008 20:20:02 -0500 3, 20 -- Received: from [78.148.233.230] (helo=[127.0.0.1]) 3, 20 -- by stoat.shef.ac.uk with esmtpsa (TLSv1:RC4-MD5:128) 3, 20 -- (Exim 4.68) 3, 20 -- (envelope-from {s.miao-at-sheffield.ac.uk} ) 3, 20 -- id 1JjOyn-0000Jj-H9 3, 20 -- for Microscopy-at-Microscopy.Com; Wed, 09 Apr 2008 02:20:01 +0100 3, 20 -- Message-ID: {47FC19BB.3060003-at-sheffield.ac.uk} 3, 20 -- Date: Wed, 09 Apr 2008 02:19:55 +0100 3, 20 -- From: Shu Miao {s.miao-at-sheffield.ac.uk} 3, 20 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 3, 20 -- MIME-Version: 1.0 3, 20 -- To: Microscopy-at-Microscopy.Com 3, 20 -- Subject: TEM- amplitude and frequency of HT center on Jeol-2010F 3, 20 -- Content-Type: text/plain; charset=UTF-8; format=flowed 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-S0phie-Scan: no ==============================End of - Headers==============================
Hello: I would like to know the synthetic method of polishing diamond suspension or paste (commercial products). I think that most are synthesized by CVD method. Is there any other synthetic method of polishing diamond? Please advise.
Dear Peggy- You didn't say which processor you have. We have a TissueTek VIP150. It stores up to 9 protocols so that we have a variety of conditions available to us, depending on the samples being processed. Just as for EM, different types of samples (size, tissue type, hardness) require different protocols. Since we are a Core Facility, we have some clients who do their own processing and some who, in order to save money (we charge per run of the machine), collect samples until they have a sizable batch. Teh machine hold up to 150 plastic tissue cassettes. These people often hold their samples in 70% EtOH until they bring them down, so some of our protocols start there, rather than with formalin. You are right, you should not jump directly into 95% ethanol. Our alcohol steps are 50, 70, 85, 95,95, 100,100 and then into Citrasolve (a less toxic xylene replacement based on d-limonene) then paraffin. Times vary, but ours range from 15min/alcohol up to 1 hr/alcohol, with up to 1 hr in each of 2 paraffin steps. I hope this is helpful, Lee -- Lee Cohen-Gould Electron & Optical Microscopy Core Facilities Weill Cornell Medical College (212)746-6146 Rms A-105, LC-207 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
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Email: kimmich46-at-gmail.com Name: rob kimmich
Organization: amateur
Title-Subject: [Filtered] used microscopes
Question: I have a turn of the twentieth century brass B&L compound scope with 10X and 40X objectives. It works well. I would like to donate it somewhere, rather than send it to be melted down.
I wonder if someone of the list has already worked with this microscope. Has someone a feedback to share? What is the vacuum necessary in the column and specimen chamber? Is a low-vacuum possible? What are the qualities and drawbacks of this system? What about EDX analysis?
Stephane
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
I have not used one of these instruments yet, but I can easily answer one of your questions.
XEDS analysis is NOT possible using the He Ion microscope.
The reason for this is that the kinetic energy of the He Ion beam is insufficient to ionize the inner shells of the respective atomic species. You will need an Ion beam accelerated to MV not KV to generate characteristic x-rays. However other types of analysis based upon the energy of the backscattered ion beam might be possible.
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 5, 11 -- From zaluzec-at-microscopy.com Thu Apr 10 07:55:40 2008 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3ACtdrg030062 5, 11 -- for {microscopy-at-microscopy.com} ; Thu, 10 Apr 2008 07:55:40 -0500 5, 11 -- Mime-Version: 1.0 5, 11 -- Message-Id: {p06240800c423bba10f79-at-[206.69.208.22]} 5, 11 -- Date: Thu, 10 Apr 2008 07:55:38 -0500 5, 11 -- To: microscopy-at-microscopy.com 5, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 5, 11 -- Subject: [Re]]: He Ion microscope Carl Zeiss 5, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
How about something like Ion Backscattered Diffraction, a word swap equivalent to electron backscattered diffraction (EBSD). Is this a possibility? Is it useful?
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
An excellent article on this subject by R. Schwarzer can be found in the January issue of Microscopy Today, pg 34. The Acronym swap has "Crystal Orientation Maps", or COM replacing EBSD.
Ron Anderson, MT Editor
mmcheath-at-syr.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } How about something like Ion Backscattered Diffraction, a word swap } equivalent to electron backscattered diffraction (EBSD). Is this a } possibility? Is it useful? } } TIA } Mike } } } ******************************************************************** } Michael M. Cheatham } 312 Heroy Geology Laboratory Phone (315)-443-1261 } Syracuse University Fax (315)-443-3363 } Syracuse, NY 13244-1070 } } email:mmcheath-at-syr.edu } http://earthsciences.syr.edu } } owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html } owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html } owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html } owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html } ******************************************************************** } } ------ Forwarded Message } X-from: {zaluzec-at-microscopy.com} } Reply-To: {zaluzec-at-microscopy.com} } Date: Thu, 10 Apr 2008 08:04:18 -0500 } To: {mmcheath-at-syr.edu} } Subject: [Microscopy] [Re]]: He Ion microscope Carl Zeiss } } microscopy-at-microscopy.com } } ------ End of Forwarded Message } } } ==============================Original Headers============================== } 10, 19 -- From mmcheath-at-syr.edu Thu Apr 10 08:11:55 2008 } 10, 19 -- Received: from SUEXCL-02.ad.syr.edu (suexbe-02.ad.syr.edu [128.230.108.46]) } 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3ADBtC7010689 } 10, 19 -- for {microscopy-at-microscopy.com} ; Thu, 10 Apr 2008 08:11:55 -0500 } 10, 19 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu ([128.230.108.49]) with Microsoft Exchange Server HTTP-DAV ; } 10, 19 -- Thu, 10 Apr 2008 13:11:55 +0000 } 10, 19 -- User-Agent: Microsoft-Entourage/11.3.3.061214 } 10, 19 -- Date: Thu, 10 Apr 2008 09:11:53 -0400 } 10, 19 -- Subject: FW: [Microscopy] [Re]]: He Ion microscope Carl Zeiss } 10, 19 -- From: Michael Cheatham {mmcheath-at-syr.edu} } 10, 19 -- To: {microscopy-at-microscopy.com} } 10, 19 -- Message-ID: {C4238A59.1C407%mmcheath-at-syr.edu} } 10, 19 -- Thread-Topic: [Microscopy] [Re]]: He Ion microscope Carl Zeiss } 10, 19 -- Thread-Index: AcibDHG6sIw+zAb/Ed2wKQAWy6AryQ== } 10, 19 -- In-Reply-To: {200804101304.m3AD4IrA007515-at-ns.microscopy.com} } 10, 19 -- Mime-version: 1.0 } 10, 19 -- Content-type: text/plain; } 10, 19 -- charset="US-ASCII" } 10, 19 -- Content-transfer-encoding: 7bit } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 4, 19 -- From randerson20-at-tampabay.rr.com Thu Apr 10 09:50:10 2008 4, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3AEo87Z026517 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 10 Apr 2008 09:50:09 -0500 4, 19 -- Received: from [127.0.0.1] (really [24.73.73.214]) 4, 19 -- by hrndva-omta01.mail.rr.com with ESMTP 4, 19 -- id {20080410145008.TLOS13774.hrndva-omta01.mail.rr.com-at-[127.0.0.1]} ; 4, 19 -- Thu, 10 Apr 2008 14:50:08 +0000 4, 19 -- Message-ID: {47FE291E.8060702-at-tampabay.rr.com} 4, 19 -- Date: Thu, 10 Apr 2008 10:50:06 -0400 4, 19 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 4, 19 -- User-Agent: Thunderbird 2.0.0.12 (Windows/20080213) 4, 19 -- MIME-Version: 1.0 4, 19 -- To: mmcheath-at-syr.edu, Listserver {Microscopy-at-Microscopy.Com} 4, 19 -- Subject: Re: [Microscopy] FW: [Re]]: He Ion microscope Carl Zeiss 4, 19 -- References: {200804101312.m3ADCHft010913-at-ns.microscopy.com} 4, 19 -- In-Reply-To: {200804101312.m3ADCHft010913-at-ns.microscopy.com} 4, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Many thanks to all who responded to my query, in fact I found trichloroethylene (1,1,2- Trichloroethene) to be more effective than either toluene or isopropanol for dissolving the Santovac that was left on the DP parts after I had heated them to 60 deg and wiped them with tissues.
I imagine that the more-widely-available perchloroethylene (tetrachloroethene) would work just as well.
Then I rinsed with isopropanol and dried in the 60deg oven before reassembly.
thanks again
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 9, 26 -- From r.sims-at-auckland.ac.nz Thu Apr 10 15:22:00 2008 9, 26 -- Received: from mailhost.auckland.ac.nz (curly.its.auckland.ac.nz [130.216.12.33]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3AKLt9B017677 9, 26 -- for {microscopy-at-microscopy.com} ; Thu, 10 Apr 2008 15:22:00 -0500 9, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id BD6DF9C73C 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2008 08:21:54 +1200 (NZST) 9, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 9, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 9, 26 -- by localhost (curly.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 9, 26 -- with ESMTP id VbdScT8zEyWY for {microscopy-at-microscopy.com} ; 9, 26 -- Fri, 11 Apr 2008 08:21:54 +1200 (NZST) 9, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 9, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 9AD3B9C736 9, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Apr 2008 08:21:54 +1200 (NZST) 9, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 9, 26 -- To: microscopy-at-microscopy.com 9, 26 -- Date: Fri, 11 Apr 2008 08:24:51 +1200 9, 26 -- MIME-Version: 1.0 9, 26 -- Subject: Cleaning up Santovac 9, 26 -- Message-ID: {47FF2053.20971.253554-at-r.sims.auckland.ac.nz} 9, 26 -- Priority: normal 9, 26 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 26 -- Content-type: text/plain; charset=US-ASCII 9, 26 -- Content-transfer-encoding: 7BIT 9, 26 -- Content-description: Mail message body ==============================End of - Headers==============================
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Email: mbisher-at-princeton.edu Name: Peggy Bisher
Organization: Princeton University
Title-Subject: [Filtered] Potassium Permanganate Fixation - Collection of Responses
Question: Tina Carvalho thought it would be a good for me to collect all the responses I received regarding my question about this topic. So here they are:
1. Joel Sheffield (jbs-at-temple.edu):
Permanganate was used as a plant cell fixative ñ look up papers by Mollenhauer from the 1960ís, and also by J.D. Robertson in his studies of membrane structure ñ those papers are from the early 1960ís. The original paper was by Luft, J.H.. J. Biophys. Biochem. Cytology (the precursor to J. Cell Bio) 2:799-801 (1956) You can probably get some insight from issues of the Journal of Cell Biology at that time.
I also have a copy of a book by Clinton Dawes from 1988 that compiles many of the techniques. The book was published by Ladd, the EM supply company, and was called ìIntroduction to Biological Electron Microscopyî. I used it for many years when I taught an EM course here.
2. Russell Spear (rzs-at-plantpath.wisc.edu)
} From Hyat 1970 Principles and Techniques of Electron Microscopy, Luftsís Buffered KMnO4
1.2% Potassium permanganate 1 part Buffer of choice
pH 7.4 ñ 7.6 fix 15 min ñ 12 hr at 0C, rinse in cold 25% EtOH, warm to room temp in fresh 25% EtOH, dehydrate as usual. Remember using for plant material a couple of times made for great contrast but had to be careful not to overfix as everything goes dark.
Can be combined with glut, but have never tried it.
3. Gregg Sobocinski (greggps-at-umich.edu) University of Michigan
Consider checking out any book by M.A. Hyatt on fixation or TEM. He describes a 3% KmnO4 in 0.1-0.2M acetate buffer pH 5.0 or in a Krebs-Ringer glucose solution at pH 7.0 for neutral buffer situations. He also describes tissues that would benefit from the different buffers.
Regards, Gregg
4. Karen Bentley (Karen_Bentley-at-URMC.Rochester.edu) University of Rochester Medical Center
} From Hyattís book I found a method:
3& KMnO4 in 0.1 acetate buffer (pH 5.0) for 30 min at 4C. Rinse briefly in buffer only and stain cells in bloc with uranyl acetate. Ultrathin sections are viewed without poststaining.
OR-
You can stain the grids (nickel or gold types only) with KMnO4 solution to increase contrast of routinely processed cells. However your resin mixture (if EPON) cannot be used due to cross reactivity of the potassium perman. With NMA component of the resin mixture.
So if you used Spurrís resin or Durcopan etc. as long as there is no NMA you are fine. I usually use Spurrís resin for popping off cells from glass coverslips.
GRID STAINING METHOD: 1.0% aqueous KMnO4 (do not filter solution let mix for 15-30) Immerse grids in large drops of stain for 30 min. or so. Rinse grids thoroughly with dist. H2O If you see precipitate on the grids you can remove that with a 0.025% solution of citric acid for 0.5-1 minute only. Rinse dist. H20.
Good Luck! Karen Bentley
5. Leona Cohen-Gould (lcgould-at-med.cornell.edu) Weill Cornell Medical College
Iíve used Pot. Permang. With great success to fix yeast, but not with cultured cells. I have just tried it with mycobacteria Ö weíre cutting the blocks today, so I donít yet know how it worked.
The protocol I use for yeast is: 2% KMnO2 (aqueous) for 30 min water wash for 30 min 1% sodium metaperiodate (aq) for 30 min water wash 15 min then I buffer wash, osmicate, etc. as usual
If you want to improve membrane preservation, you can stick with aldehydes and use a formula developed by Karnovsky (1968, J Cell Biol vol89, abs. 418). Itís a modified Bouinís fix containing 0.02% picric acid to a solution of 4% pfa, 2.5% glut in 0.1 M Na-caco buffer at pH 7.2,
Picric acid in the lab will make your safety people unhappy. I have had the same 100gm bottle in my hood for } 12 years. I keep the crystals well covered with water and draw off the saturated solution to use in my fix, topping off the water as the level drops. I make sure not to allow any crystals to collect around the rim of the bottle. Its when the crystals dry and lose their water of hydration that they become explosive.
I also use potassium ferricyanide in my osmium to further enhance membranes.
Good Luck, Lee.
6. Michael Standing (provoem-at-msn.com) BYU Microscopy Lab
I have a book that has information about permangenate stains. I would be happy to fax a copy of the section to you if you want to send me your fax number. The book is:
Pease, D.C. ìHistological Techniques for Electron Microscopyî second edition, Academic Press, New York and London, 1964
The section gives several protocols and indicates that buffered v. unbuffered fixatitves doesnít make much difference. And unbuffered protocol by Tahmisian (1964) is:
KMnO4 1.3 gm NaCl 4.5 gm Distilled water to make 100ml
Put the sample in fixative for 15 minutes to 2 hours (time is important and may need to be experimented with).
Mollenhauer (1959) recommends unbuffered 2-5% solutions at room temperature for the same above times.
I have received a request from a user who wants to have an image of SEM emission image along with EDS results. The user claims that he wants to use that information to estimate error in EDS analysis. This is the first time I have heard something like this. What would be the effect of e-beam alignment on EDS results, as long as it is aligned well (best we can do manually) along the optic axis?
At 06:52 -0500 12/04/08, celikaktas-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Which error is he talking about?
If he is refering to spatial resolution, this is largely (entirely?) dependent on the sample. -- Larry Stoter
(Working on a Microsoft-free computer)
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==============================Original Headers============================== 5, 16 -- From larry-at-cymru666.plus.com Sat Apr 12 11:58:58 2008 5, 16 -- Received: from ptb-relay03.plus.net (ptb-relay03.plus.net [212.159.14.214]) 5, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3CGwvDi005680 5, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 12 Apr 2008 11:58:57 -0500 5, 16 -- Received: from [87.114.137.14] (helo=[192.168.1.2]) 5, 16 -- by ptb-relay03.plus.net with esmtp (Exim) id 1Jkj43-00048X-Vc; Sat, 12 Apr 2008 17:58:56 +0100 5, 16 -- Mime-Version: 1.0 5, 16 -- Message-Id: {p06240800c4269a159e34-at-[192.168.1.2]} 5, 16 -- In-Reply-To: {200804121152.m3CBqBJC019926-at-ns.microscopy.com} 5, 16 -- References: {200804121152.m3CBqBJC019926-at-ns.microscopy.com} 5, 16 -- Date: Sat, 12 Apr 2008 17:58:40 +0100 5, 16 -- To: celikaktas-at-gmail.com, Microscopy-at-MSA.Microscopy.Com 5, 16 -- From: Larry Stoter {larry-at-cymru666.plus.com} 5, 16 -- Subject: Re: [Microscopy] Effect of beam alignment on EDS results 5, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 16 -- X-Plusnet-Relay: 79a365fbd37fc5329cc6bab9cc1a7213 ==============================End of - Headers==============================
I can readily show you BSE images (and x-ray maps) of polished samples taken at low magnification that show brightness variation across the field. There is a substantial fall-off in brightness toward the corners. Presumable the bright area is centered in the field. If not then there is need for alignment.
However, I am afraid that this client is straining at a gnat and swallowing a camel.
Are the EDS analyses being done without normalizing the results? If so, the brightness variation will affect the totals or any element determined by difference. I expect the results are being normalized so there would be no effect. Does your client understand the distinction? With normalization, a phase probed at the corner of the field would report the same composition as the same phase probed at the center of the field.
Is the spectrum being collected from a homogeneous area? Perhaps the user wants to be sure that all phases are being represented evenly across the field of view. That is understandable - but wrong! EDS matrix corrections assume a homogenous sample volume. Let's assume an Ni-Al sample prepared by butting up chunks of the two elements against each other. If you are correcting the Al emission from one side for the effect of Ni atoms on the other side, that would be wrong. The generation of x-rays and they interaction with both Al and Ni atoms would only occur along a narrow band of the interface between the two phases. Otherwise, the spectrum is just the sum of the signals from the pure phases. Virtually no correction would be necessary.
Is the client aware of the accuracy limitations of EDS? If they are inter-comparing analyses taken on the same system, they may be able to make some sensitive comparisons. However, counting statistics probably limit repeatability to a few tenths of a percent or worse. Absolute accuracy could be off by a few percent. What level of improvement will result from their exercise?
I maintain that EDS is quite a powerful technique - when done correctly. My impression is that the effect your client is concerned about is probably the least of their worries.
Warren Straszheim Materials Analysis Lab Iowa State University
________________________________________ X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] Sent: Sat 4/12/2008 6:39 AM To: wesaia-at-iastate.edu
This Question was submitted to Ask-A-Microscopist by (charlieangelwannabe-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, April 12, 2008 at 16:16:18 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both charlieangelwannabe-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Organization: San Joaquin Delta College CMAS Department
Education: Undergraduate College
Location: Stockton, CA, USA
Title: SEM Sample Prep. of Opals
Question: To preform a resolution study with Opals, with the use of a SEM 6100 EDS, how would the specimen be prepared , prior to mounting on the aluminum stub?
This Question was submitted to Ask-A-Microscopist by (charlieangelwannabe-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, April 12, 2008 at 16:24:48 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both charlieangelwannabe-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Organization: San Joaquin Delta College CMAS Department
Education: Undergraduate College
Location: Stockton, CA, USA
Title: SEM sample prep of materials
Question: I am preforming a special studies assignment of the effects of force on the surface of metals. If a sample was obtained of an expelled bullet what would be the best way to prepare the surface for viewing with the SEM 6100 JOEL? Also if Gun Shot Residue (GSR) was to be collected for SEM analysis would replication tape be a good source to collect the sample and how would one go about this form of preparation?
This Question was submitted to Ask-A-Microscopist by (charlieangelwannabe-at-yahoo.com) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, April 12, 2008 at 16:29:05 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both charlieangelwannabe-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Organization: San Joaquin Delta College CMAS Department
Education: Undergraduate College
Location: Stockton, CA, USA
Title: SEM sample prep of materials
Question: When preparing quartz to be viewed and analyzed with the use of a SEM 6100 JEOL could the sample be fractured and attached to an aluminum stub with the use of double sticky carbon tape and carbon coated with the evaporator? Or is there a better approach to this study of a quartz sample?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Kochde83-at-gmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, April 8, 2008 at 21:28:54 ---------------------------------------------------------------------------
Email: Kochde83-at-gmail.com Name: Diana Koch
Organization: Cincinnati Children's Hospital and Medical Center
Education: Undergraduate College
Location: Cincinnati, Ohio, USA
Question: I am interested in becoming an expert at confocal and Electron Microscopy. What is the best educational path to achieve these goals? Are there certifications for microscopy? Are there recognized schools or training courses/ institutions that specialize in microscopy training? Whatever information you can provide me will be most helpful. Thank you.
This client wants the screen capture image of the "electron beam" (how well the e-beam is centered, aligned) that we can see during alignment process. I'm not sure if this could be used for estimation of error for EDS results at all.
Of course, I obtain SEM and BSE images of the areas that I have examine for EDS work.
Kind Regards, Ayten.
On Sat, Apr 12, 2008 at 6:37 AM, Ayten Celik-Aktas {celikaktas-at-gmail.com} wrote: } Dear all, } } I have received a request from a user who wants to have an image of } SEM emission image along with EDS results. The user claims that he } wants to use that information to estimate error in EDS analysis. This } is the first time I have heard something like this. What would be the } effect of e-beam alignment on EDS results, as long as it is aligned } well (best we can do manually) along the optic axis? } } Kind Regards, } Ayten Celik-Aktas }
I will second Warren's assessment of the situation. It sounds like your client is concerned about the molehills but is overlooking the mountains.
Perhaps your client knows something about a different analytical technique (such as WDS, where beam alignment is important to preserve the Rowland circle geometry) and is trying to bring that bit of knowledge to EDS. Perhaps your client is simply working from specifications written by someone else and is holding to them because they are required to keep their procedures the same over time. Perhaps some earlier EDS analyst included this in a report, and now your client believes it is always important to know. Perhaps they're trying to "break the rules" of EDS, as Warren described, and are hoping a heterogeneous region will be evenly illuminated by the beam. There are many possibilities about why they want this information, but that's not the most important question right now...
The question at this point, Ayten, is: What would you like us to help you do?
I see two basic options:
1) Do you want ideas about a way to image your beam alignment and simply satisfy their request?
2) Do you want to educate them and convince them that this really isn't the biggest source of error?
Option #1 is clearly the easiest one: you can satisfy your client and simply be finished with it. If I was asked to do this, I would either: (A) place a cathodoluminescent mineral in the microprobe, align the luminous spot with the cross-hairs of the visible-light microscope that is aligned with our electron optical column, capture an image of this, and give that image to the client; or (B) burn a hole in or leave a carbon deposition spot on a sample, capture an image of how the mark sits at the center of the electron image, and give that image to the client. Those methods aren't perfect, but I think that they would satisfy most clients who'd ask to know such a thing.
Option #2 is probably the more "responsible" thing to do, but it is also going to be more difficult. I've been in a similar situation before. I've had to deal with a corporate client who wanted to superalloy samples analyzed, and they wanted to start using my lab, rather than the lab they had been using, because my lab was within courier distance. The first step, though, was to show that I could give the same results as the other lab, and I had to use the procedures established by that other lab. The problem was that the other lab was using procedures with which I didn't agree, including rastering the beam across a heterogeneous area to get an "average" composition. This heterogeneous area included metals with serious issues of absorption and fluorescence, so the correction procedures were essentially being lied to. I voiced my concerns with the client, but they didn't get it. Nor did they understand that slightly different ZAF methods produced different results because of these ill- chosen procedures. In the end, the client didn't end up using our lab because consistency, not a better procedure, was more important to them. Educating a new corporate client about their misconceptions can be highly challenging, and sometimes they really don't want to know.
Option #2 is the better for all of us as it would improve your client's understanding of X-ray microanalysis. But I certainly wouldn't blame at all you for following Option #1 and just trying to give the client what they want (or think they want).
Or you could try to do both Options #1 and #2 -- give them what they want *and* educate them why it is not important.
So let us know what you'd like to do, Ayten, and we'll be better able to help you with ideas and references.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
On Apr 13, 2008, at 1:48 PM, celikaktas-at-gmail.com wrote:
} This client wants the screen capture image of the "electron beam" (how } well the e-beam is centered, aligned) that we can see during alignment } process. I'm not sure if this could be used for estimation of error } for EDS results at all. } } Of course, I obtain SEM and BSE images of the areas that I have } examine for EDS work. } } Kind Regards, } Ayten
That makes it clearer. I could not see how the alignment would have any effect on EDS accuracy. Maybe it does. It would affect probe current and volumetric interaction most likely. If it affects accuracy, how so?
Depending on the SEM, getting a beam image can be easy. With LEO/Zeiss, use Emission mode. With FEI/Philips, use X-Over mode. Not informed about other brands.
gary g.
At 11:43 AM 4/13/2008, you wrote:
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==============================Original Headers============================== 7, 21 -- From gary-at-gaugler.com Sun Apr 13 22:11:33 2008 7, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m3E3BWL6010183 7, 21 -- for {microscopy-at-microscopy.com} ; Sun, 13 Apr 2008 22:11:33 -0500 7, 21 -- Message-Id: {200804140311.m3E3BWL6010183-at-ns.microscopy.com} 7, 21 -- Received: (qmail 3969 invoked from network); 13 Apr 2008 20:11:32 -0700 7, 21 -- Received: by simscan 1.1.0 ppid: 3963, pid: 3965, t: 0.0962s 7, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 21 -- by smtp2 with SMTP; 13 Apr 2008 20:11:32 -0700 7, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 21 -- Date: Sun, 13 Apr 2008 20:13:19 -0700 7, 21 -- To: celikaktas-at-gmail.com 7, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 21 -- Subject: Re: [Microscopy] Clarification. Re: Effect of beam alignment 7, 21 -- on EDS results 7, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 21 -- In-Reply-To: {200804131843.m3DIhGI7004827-at-ns.microscopy.com} 7, 21 -- References: {200804131843.m3DIhGI7004827-at-ns.microscopy.com} 7, 21 -- Mime-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-4803423B ==============================End of - Headers==============================
We had various automatic fibre length measurements programs that cost us thousands back in the 1990’s – the best one was called FIBRE and part of the hardwired Joyce Loebl/Applied Imaging Magiscan Colour image analysis system - that in some ways still knocks the spots of many modern image analysis programs available these days [but then it did cost £80,000].
As with any automatic image analysis it’s the microscope image quality not the Fibre software program that’s paramount. With phase contrast or DIC we could easily automatically measure fibre length, but to be honest with the amount of editing the thresholded binary image needed to remove spurious objects and fill in missing bits, it was far easier just to draw a line along the fibre length and measure that instead [so that’s what we often did]. This was even when using object classifiers to automatically delete obvious non-fibrous objects (our recovered test material wasn’t always ‘fibers’ once it started to dissolve in the lung). There were also problems with overlaying fibres and fibre ends (going beyond the frame) that needed manually editing as well. And all that was when we had a perfect image of the fibres suitable for thresholding/detection – not always easy to achieve.
If freebie ImageJ can’t threshold (i.e. detect) all the fibres without the need to manually edit the binary image then chances are a far more expensive program like MetaMorph and Image Pro Plus won’t do a lot better (although it will offer far better automatic filtering/removal of obviously non-fibrous detected objects). Most Image Analysis programs still mainly rely on algorithms developed twenty or more years ago, and recent advances concentrate more on image processing to improve detail (e.g. deconvolution) – plus most binary image [thresholded] editors seem very poor compared to these old image analysis systems, and today I’m often reduced to using Photoshop rather than the editing tools provided [which works]. We are still a long way from getting machines to distinguish fibres in a busy image (although the wood fibre industry has developed algorithms that try to do this, but I don’t know how successful they are at full automation). Magiscan Fibre appeared to skeletize the thesholded fibre and then did a classy series of ‘perpendicular’ lines along the ‘horizontal’ curved fibre length to measure the diameter as well – it looked really cool on the screen with a perfect test image and we used it to impress the visitors [and then measured it all semi-manually later when they had gone home].
We used an optical on-screen light-pen for drawing lines/editing and these days a Wacom Intuos/Cintiq stylus tablet would do the same thing (a standard mouse just isn’t as good). We abandoned the Fibre program (ie. never used it) in the end, particularly as it always got the fibre diameter wrong due to optical effects with the phase contrast halo around our bifringent MMVF fibre samples [not the programs fault], and we finally went to mainly imaging under SEM (manually measuring the fibre length/diameter live). You couldn’t easily threshold fibres when they were viewed under SEM as the background [Nucleopore filters*] was often very difficult to distinguish from the fibres. Our fibres were amphibole/serpentine asbestos and MMVF replacements (glass/rockwool and ceramic). Fibre counting was always far easier by eye, although imaging on the PC VDU with a frame overlaid was far better than peering down the microscope (we manually scored all the fields onto paper and then input the field counts into a spreadsheet – so that we could trace all the measurements under QA & ISO-9001). All fibre size data was directly accumulated on the PC.
Most image analysis programs have macros that semi-automate almost all of the process anyway, pausing only at the thresholding/editing stages. Plus you can get Visual basic add-ins that allow more complex programming (and more of your time). Image Pro Plus http://www.mediacy.com/index.aspx?page=IQmaterialsParticle has a specific particle, including fibres, measurement module (called IQMaterials) that should be similar, possibly better or possibly worse, than our old Magiscan FIBRE program – so check that out. Never tried it, but I expect the above still applies – you do need that good image to start with (so sample preparation and microscope setup are paramount).
Regards
Keith
* http://www.sterlitech.com/products/membranes/polycarbonate/PCTEMembrane.htm? gclid=CJOTwoOI05ICFQQx1AodSzmVGA --------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
I wonder if there is a good image analysis tool or method that can measure glass/carbon fiber (those found in polymers as fillers) length automatically. Typically we need 300+ data points/sample for statistical analysis. We use a semi-auto method and only the data log-in portion is automatic.
My understanding is that overlapping of fibers (which is unavoidable) makes automated measurements difficult. I'd like to ask for your suggestion/advice. Thank you very much!
The Microscopy Society of America did have a TEM certification, but I can't find it on their website anymore. Courses: 2-year Associates degree programs are offered by Madison Area Technical College, in Madison, Wisconsin http://matcmadison.edu/matc/ASP/showprogram.asp?programnumber=106361 and by San Joaquin Delta Community College in Stockton, CA http://www.deltacollege.edu/dept/electmicro/index.html Both are excellent programs. LeHigh University offers excellent short courses in various microscopies, but not (I think) confocal http://www.lehigh.edu/microscopy/ McCrone Institute has microscopy short-courses, but I don't know if they cover what you want. http://www.collegeofmicroscopy.com/?gclid=CICoqczM2pICFQUpIgodeywC5w
There are more: Two particularly good (and intensive) programs run by Kent McDonald (UC Berkeley) on cryoEM, and Jim Pawley (UW Madison), a live-cell imaging/confocal short course. There are others, more than I can remember or have links for. You should get replies about these, and they are regularly announced on the microscopy listserver. You should join it.
And, Central Michigan U. (see my signature) has a microscopy concentration within the Biology major and coursework in light, confocal, SEM and TEM. These courses could also be taken outside of the Biology major (say, as a returning student), or as part of a M.S. program. http://microscopy.bio.cmich.edu/
Phil
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The MSA Certification Board is alive and well and vibrant. Please contact: Dr John Petrali, its current Chairperson at: "Petrali, John P Dr USAMRICD" {john.p.petrali-at-us.army.mil} . He will be more than happy to fill you in. Lee (an active member of the Cert. Board)
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-- Lee Cohen-Gould Electron & Optical Microscopy Core Facilities Weill Cornell Medical College (212)746-6146 Rms A-105, LC-207 http://www.cornellcelldevbiology.org http://www.cornellbiochem.org
I need to change the drive belt on our Ultracut microtome. This is a real oldie, an AO Ultracut circa 1981.
I have changed the belt before, but it has been a long time. I think the last time I just found an O-ring that fit and did it. Now I need to do it again and was wondering if there is an 'official' part I should use or special kind of O-ring that might last longer. Also, don't have anything that says what size to use, last time I just guessed.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to make a donation. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 7, 20 -- From jmkrupp-at-ucsc.edu Mon Apr 14 15:22:41 2008 7, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3EKMeY9009184 7, 20 -- for {microscopy-at-microscopy.com} ; Mon, 14 Apr 2008 15:22:40 -0500 7, 20 -- Received: from [128.114.125.117] (HELO ucsc.edu) 7, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 7, 20 -- with ESMTPS id 32722069 for microscopy-at-microscopy.com; Mon, 14 Apr 2008 13:22:40 -0700 7, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 7, 20 -- with PIPE id 81389588; Mon, 14 Apr 2008 13:22:39 -0700 7, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 7, 20 -- Received: from [128.114.25.59] (account jmkrupp-at-ucsc.edu HELO [128.114.25.59]) 7, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 7, 20 -- with ESMTPA id 81389544 for microscopy-at-microscopy.com; Mon, 14 Apr 2008 13:22:38 -0700 7, 20 -- Mime-Version: 1.0 7, 20 -- Message-Id: {p06230906c4296bfdf8fd-at-[128.114.25.59]} 7, 20 -- Date: Mon, 14 Apr 2008 13:22:35 -0700 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 7, 20 -- Subject: Ultracut drive belt? 7, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Also, Merritt College in Oakland now has a one year (night and weekends) Certificate in Bioscience Microscopy. Check it out at www.merritt.edu/microscopy. Gisele Giorgi
I am looking for a TEM stain that is specific to proteins containing the post-translationally modified amino acid 3,4- dihydroxyphenylalanine (DOPA). I am aware of a number of LM stains, but have not been able to find anything for TEM. I am interested in detecting this amino acid both in its native form (i.e. unoxidized) and after cross-linking (following oxidation to DOPA quinone) in the adhesive apparatus of mussels.
Any ideas or suggested resources would be appreciated. Eli
Eli Sone Institute of Biomaterials and Biomedical Engineering (IBBME) University of Toronto Rosebrugh Building, Room 405 164 College St. Toronto, Ontario M5S 3G9
Don't ever ditch that baby! They can live forever! Helmut and Mario at MOC just serviced ours and it cuts like the day it was installed!
There is an official part. I have the number at work, but even better, just contact MOC and Helmut can get it for you. The official part is kind of pricey, about $70 I think, but how many do you use? I have MOC contact info on our "Open_Cut" page: http://people.umass.edu/dac/projects/Open_Cut/Ultracut_Service.html
By the way, please join our group if you would like; it is a somewhat inactive group of us who have older microtomes - a place to share such information as this.
I found some belts that might work - check Stock Drive Products https://sdp-si.com/eStore/ click "belts & cables" and "round endless belts". Cheaper, but you are experimenting....
What I did as an emergency fix - seemed to work fine - was to get some green 1/4" polycord belt from Small Parts, Inc.; it is a bit stiffer and the minimum specified radius is a bit larger than the servo pulley, but it was working fine. This stuff is sold by the foot, cut to length and the ends heated just to melting, and gently pressed together in a v-channel (for alignment) and then the "flash" is trimmed. Again, it is stiffer and probably should be considered a short-term fix.
Good luck!
Dale Callaham Umass -at- Amherst
jmkrupp-at-ucsc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } I need to change the drive belt on our Ultracut microtome. This is a } real oldie, an AO Ultracut circa 1981. } } I have changed the belt before, but it has been a long time. I think } the last time I just found an O-ring that fit and did it. Now I need } to do it again and was wondering if there is an 'official' part I } should use or special kind of O-ring that might last longer. Also, } don't have anything that says what size to use, last time I just } guessed. } } Thanks } } Jon
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Email: yngli-at-ucdavis.edu Name: YING LI
Organization: University of Carlifornia Davis
Title-Subject: [Filtered] Anyone knows how to use the nanoprobe mode in TEM Phillips CM12?
Question: Anyone knows how to use the nanoprobe mode in TEM Phillips CM12? If you know can you just tell me the simple procedure?
I just want to use it to get the some micro-diffraction pattern.
This Question was submitted to Ask-A-Microscopist by (kathy.flynn-at-canyons.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, April 14, 2008 at 13:22:19 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both kathy.flynn-at-canyons.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
This Kathy Flynn from College of the Canyons, a community college in Santa Clarita, CA. Our Math&Science division is purchasing a small table-top electron microscope to use in lower division undergraduate labs. We are looking at the Hitachi TM-1000 and Evex SX-1500. Could you please comment on ease-of-use and capabilities of these instruments? Any help you provide would be greatly appreciated. Thanks.
Stephane, I am from the ALIS unit of Carl Zeiss SMT, the manufacturer of the Helium Ion Microscope (HIM). In response to your questions:
The specimen chamber is held at a vacuum similar to what would be found in
a FIB or high vacuum SEM: around 1E-7 torr. The gun base pressure must be at near UHV (1E-9 torr); however, it is much higher when running the ion gun due to the flow of the source gas to the emitter. There is differential pumping in the column to maintain the desired pressure in each region.
Low vacuum is generally not possible in any ion beam microscope because the scattering cross section is much higher for an ion beam traversing a gas than for an electron beam. Above a pressure of about 1E-4 torr significant spreading of the primary beam is noticeable. However, at least
one of the two reasons that one typically pursues low vacuum operation is charge control, and this is accomplished in a ion beam tool by the use of a low energy electron flood gun. Sample hydration is the other driver for low vac, but there is no immediate solution for that application.
Three notable features of HIM are the small probe size, the reduced interaction volume with the sample, and the different contrast mechanisms created by the signal generation from a primary helium ion beam. The high brightness of the source (at least 6E9 at voltage) allow for sub-nanometer probes to be formed. A probe size of 0.25nm should be achievable, 0.5nm has been measured. The low energy of the secondary electrons created by an impinging ion beam in a sample translates into a small escape depth (a few nm). Combined with the small lateral size of the probe, this means that the volume probed per pixel is small (no BSE's or SE-II's). Finally, the contrast mechanisms are qualitatively different for an ion beam generated image, giving different image information - and generally more gray levels than an SEM image. It is also possible to collect backscattered helium ions for imaging (since the ion mass is low), yielding a further type of analysis.
To answer another question posed, there is an analogue for ions to EBSD: it is referred to as the ion blocking pattern, IBP. One can read about it in the January, 2008 article in Microscopy Today (p.34).
Surfaces do need to be kept clean for the technique to work well - the microscope can both create and see hydrocarbon deposition better than an SEM. This can be addressed through engineering, however, and is not a fundamental limitation. Sputtering damage is minimal, but not zero. Helium
implantation damage can occur, but this is usually at a dose much higher than is required for imaging.
Nestor already answered correctly about EDX. However, the energy analysis of backscattered helium ions can provide elemental analysis akin to RBS. We are developing this capability.
Regards, Larry Scipioni Director of Applications Research ALIS Business Unit Carl Zeiss SMT, Inc.
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==============================Original Headers============================== 14, 24 -- From l.scipioni-at-smt.zeiss.com Tue Apr 15 07:20:55 2008 14, 24 -- Received: from USNYMTASMTP01.zeiss.com (usnymtasmtp01.zeiss.com [165.254.160.245]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3FCKt3F026079 14, 24 -- for {Microscopy-at-Microscopy.Com} ; Tue, 15 Apr 2008 07:20:55 -0500 14, 24 -- Received: from USMASN01.zeiss.org ([10.18.85.5]) 14, 24 -- by USNYMTASMTP01.zeiss.com (Lotus Domino Release 7.0.3FP1) 14, 24 -- with ESMTP id 2008041508262439-12761 ; 14, 24 -- Tue, 15 Apr 2008 08:26:24 -0400 14, 24 -- To: Microscopy-at-Microscopy.Com 14, 24 -- Subject: [Re]]: He ion microscope Carl Zeiss 14, 24 -- Message-ID: {OF95422C0E.F0F7A576-ON8525742C.00435719-8525742C.0043D480-at-zeiss.org} 14, 24 -- Date: Tue, 15 Apr 2008 08:20:52 -0400 14, 24 -- From: l.scipioni-at-smt.zeiss.com 14, 24 -- MIME-Version: 1.0 14, 24 -- X-Mailer: Lotus Notes Release 7.0 August 18, 2005 14, 24 -- X-Disclaimed: 18363 14, 24 -- X-MIMETrack: Itemize by SMTP Server on USNYMTASMTP01/Thornwood/US/Zeiss(Release 7.0.3FP1|February 14, 24 -- 24, 2008) at 04/15/2008 08:26:24 AM, 14, 24 -- Serialize by Router on USNYMTASMTP01/Thornwood/US/Zeiss(Release 7.0.3FP1|February 14, 24 -- 24, 2008) at 04/15/2008 08:26:25 AM, 14, 24 -- Serialize complete at 04/15/2008 08:26:25 AM 14, 24 -- Content-Type: text/plain; charset="US-ASCII" 14, 24 -- Content-Transfer-Encoding: 8bit 14, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3FCKt3F026079 ==============================End of - Headers==============================
Does anyone have a side entry stage and/or a large gap objective lens pole piece for a JEOL 4000? So many have been decommissioned that there should be some pieces hiding in closets somewhere.
Thanks, Roseann
Roseann Csencsits Lawrence Berkeley Lab Donner Lab 365 1 Cyclotron Road Berkeley CA 94720 510-486-4548
==============================Original Headers============================== 5, 23 -- From rcsencsits-at-lbl.gov Tue Apr 15 16:47:22 2008 5, 23 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3FLlLf3001119 5, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Apr 2008 16:47:21 -0500 5, 23 -- X-Ironport-SBRS: 2.9 5, 23 -- X-IronPort-Anti-Spam-Filtered: true 5, 23 -- X-IronPort-Anti-Spam-Result: AvkBALm/BEiAAykYgWdsb2JhbACRWQEBECabAQ 5, 23 -- X-IronPort-AV: E=Sophos;i="4.25,662,1199692800"; 5, 23 -- d="scan'208";a="99974835" 5, 23 -- Received: from mta1.lbl.gov ([128.3.41.24]) 5, 23 -- by ironport1.lbl.gov with ESMTP; 15 Apr 2008 14:47:13 -0700 5, 23 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) 5, 23 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id m3FLlCnk007107 5, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 15 Apr 2008 14:47:13 -0700 (PDT) 5, 23 -- Message-Id: {66D98BD8-0374-4BB1-81CB-3F27834B3C19-at-lbl.gov} 5, 23 -- From: Roseann Csencsits {rcsencsits-at-lbl.gov} 5, 23 -- To: Microscopy-at-microscopy.com 5, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) 5, 23 -- Subject: 4000 side entry stage 5, 23 -- Date: Tue, 15 Apr 2008 14:47:08 -0700 5, 23 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
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Email: bloos-at-sun.ac.za Name: Ben Loos
Organization: Stellenbosch University
Title-Subject: [Filtered] resin embedded tissue
Question: Dear collegues,
this is the first time I submit a question to the list-so I hope it works.
Could anyone advice me, I have a client who had resin embedded tissue, and wants immunofluorescence staining done on it.
As i only have experience with paraffin embedded tissue, I would like to know, how the sections should be treated to "de-resin", and whether some unmasking step for better antigen exposure is needed? I have had a look, but couldn't find sufficient protocols. Could someone point me into the right direction please?
thanks a lot, your advice is much appreciated Ben
Ben Loos
Cell Imaging Unit -Fluorescence Live Cell Imaging & Flow Cytometry- Central Analytical Facility-CAF
Stellenbosch University - South Africa Department of Physiological Sciences Mike de Vries Building Office 2045 7600 Stellenbosch Tel: +27 84 893 2707 Fax:+27 21 808 3145 email: bloos-at-sun.ac.za website: www.sun.ac.za/saf
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Email: etobrien-at-email.unc.edu Name: E. Tim O'Brien
Organization: UNC-Chapel Hill
Title-Subject: [Filtered] Carolina Workshop on Force Measurements and Manipulation in Biological
Question: Carolina Workshop on Force Measurements and Manipulation in Biological Microscopy at UNC Chapel Hill on May 27-30, 2008.
The workshop is hosted by our NIH resource CISMM (Computer Integrated Systems for Microscopy and Manipulation) See http://www.cs.unc.edu/Research/nano/cismm/index.html for an overview of CISMM. The workshop consists of morning lectures that provide a framework for understanding and analyzing forces on the micro and nanoscale, and serve as an introduction to the afternoonís experiments. The afternoon sessions are hands-on laboratories where you work with live samples: single molecules of DNA, epithelial cells, and biological fibers such as fibrin fibers on structured surfaces. Experiments use laser tweezers, an integrated atomic force microscopy/optical microscopy system, and 3D magnetic systems integrated with a fluorescence confocal microscope. We also include an introduction to microfluidics. Finally, participants spend a half day learning to use some of the many free software packages available from our resource that facilitate the analysis of forces in biological systems. Registration is limited to 18, so you have lots of hands-on time with the microscopes. The $775 fee includes light breakfasts, snacks and one dinner. Our keynote speaker will be John Weisel of the Dept. of Cell & Developmental Biology at the University of Pennsylvania school of Medicine.
http://www.cs.unc.edu/Research/nano/cismm/ForcesWorkshop.htm Hope to see you there!
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (andel-at-cperi.certh.gr) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, April 16, 2008 at 04:18:45 ---------------------------------------------------------------------------
Email: andel-at-cperi.certh.gr Name: Andreas Delimitis
Organization: Centre for Research & Technology Hellas - CPERI
Education: Graduate College
Location: Thermi, Thessaloniki, Greece
Question: RE: Oil leak in rotary pump of JEOL 2011 TEM
I would like to thank all the listers who responded to the RP oil leak problem, for their quite useful advice and suggestions they provided.
Thanks a lot, Andreas
--------------------------------------------- Dr. Andreas Delimitis Associate (Application) Scientist C' Chemical Process Engineering Research Institute (CPERI) Centre for Research & Technology - Hellas (CERTH) 6th Km. Charilaou - Thermi road 57001 Thermi, Thessaloniki GREECE Tel: +30 2310 498259 E-mail: andel-at-cperi.certh.gr ---------------------------------------------
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Email: isabel.nogueira-at-ist.utl.pt Name: Isabel Dias Nogueira
Organization: ICEMS/IST (Lisbon-Portugal)
Title-Subject: [Filtered] SEM and TEM: algae in chiller
Question: Dear fellow microscopists,
We have a conventional SEM, a FEG-SEM (the most recent acquisition) and a TEM, all with water circulation provided by the same chiller. Lately we have noticed some algae forming inside the hose of the FEG-SEM, which is a bit more transparent than the rest. We have changed the water in the chiller, but with no results. My questions are: 1) Is it possible to use some product to remove these algae without damaging any part of the microscopes? If not, what can we do? 2) Do you use destilled water in such chillers?
Thank you for sharing any experience you might have on this subject, Isabel Dias Nogueira
} } } Preventing the growth of algae in cooling water systems is discussed } } in detail in my book "Vacuum Methods in Electron Microscopy" Two } } chemicals commonly used to prevent algal growth in such systems are } } Chloramine T and dichlorophene. Both of these chemicals can be } } obtained from various specialty chemical companies. You can also } } probably obtain algaecides from companies (and merchants) that sell } } water beds, and swimming pool equipment. I do not recommend the use } } of ethylene glycol for several reasons that are discussed in my book. } } Also, remember that algae require light in order to grow, and so you } } can substantially inhibit their growth by fully excluding light from } } the system (i.e. cover the reservoir with a a light-tight cover, and } } use fully opaque tubing). Changing from ordinary tap water to } } distilled water will probably not give you much of an advantage, } } except for possibly minimizing the formation of a bit of scale in the } } heated parts of a diffusion pump. However, the amount of scale } } formation should be quite limited since it is a closed system } containing a limited amount of water. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Wed Apr 16 14:42:55 2008 1, 14 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3GJgttu021638 1, 14 -- for {microscopy-at-microscopy.com} ; Wed, 16 Apr 2008 14:42:55 -0500 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY hackers.mr.itd.umich.edu ID 480656BD.5B6BE.27407 ; 1, 14 -- 16 Apr 2008 15:42:53 -0400 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240800c42c06cc0b30-at-[141.212.131.221]} 1, 14 -- Date: Wed, 16 Apr 2008 15:42:50 -0400 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy]RE: algae in water chillers 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This question, and many like it, come up from time-to-time, and the answers are frequently found in Wil's excellent book, which should, IMHO, be required reading for anyone concerned with the technical aspects of keeping EM vacuum systems working well. It is wonderfully informative, and well enough written to be a pleasure to read.
If there was a copy in every EM lab, Wil wouldn't need to so often write the sentence:
"......................... is discussed in detail in my book "Vacuum Methods in Electron Microscopy"
Buy the book!
cheers
Ritchie Sims
ps I have no commercial relationship with Wil or with the publishers or distributors of the book. I have, though, from time-to-time, exchanged cordial emails with Wil, who has been a mine of information and extremely helpful.
On 16 Apr 2008 at 14:44, bigelow-at-umich.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } } Preventing the growth of algae in cooling water systems is discussed } } } in detail in my book "Vacuum Methods in Electron Microscopy" Two } } } chemicals commonly used to prevent algal growth in such systems are } } } Chloramine T and dichlorophene. Both of these chemicals can be } } } obtained from various specialty chemical companies. You can also } } } probably obtain algaecides from companies (and merchants) that sell } } } water beds, and swimming pool equipment. I do not recommend the use } } } of ethylene glycol for several reasons that are discussed in my book. } } } Also, remember that algae require light in order to grow, and so you } } } can substantially inhibit their growth by fully excluding light from } } } the system (i.e. cover the reservoir with a a light-tight cover, and } } } use fully opaque tubing). Changing from ordinary tap water to } } } distilled water will probably not give you much of an advantage, } } } except for possibly minimizing the formation of a bit of scale in the } } } heated parts of a diffusion pump. However, the amount of scale } } } formation should be quite limited since it is a closed system } } containing a limited amount of water. } -- } Wilbur C. Bigelow, Professor Emeritus } Materials Sci. & Engr., Univ. of Michigan } Ann Arbor, Michigan 48109-2136 } e-mail: bigelow-at-umich.edu; } Fx:734-763-4788; Ph:734-975-0858 } Address mail to: 2911 Whittier Court } Ann Arbor, MI 48104-6731 }
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 17, 28 -- From r.sims-at-auckland.ac.nz Wed Apr 16 15:46:39 2008 17, 28 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3GKkcDi011224 17, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Apr 2008 15:46:39 -0500 17, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 17, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 26F2418919 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 08:46:33 +1200 (NZST) 17, 28 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 17, 28 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 17, 28 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 17, 28 -- with ESMTP id haJlth4AgUln for {microscopy-at-microscopy.com} ; 17, 28 -- Thu, 17 Apr 2008 08:46:33 +1200 (NZST) 17, 28 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 17, 28 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 0DFEB1815B 17, 28 -- for {microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 08:46:33 +1200 (NZST) 17, 28 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 17, 28 -- To: microscopy-at-microscopy.com 17, 28 -- Date: Thu, 17 Apr 2008 08:46:31 +1200 17, 28 -- MIME-Version: 1.0 17, 28 -- Subject: Re: [Microscopy] RE: algae in water chillers 17, 28 -- Message-ID: {48070E67.3162.A8089-at-r.sims.auckland.ac.nz} 17, 28 -- Priority: normal 17, 28 -- In-reply-to: {200804161944.m3GJiR5d023112-at-ns.microscopy.com} 17, 28 -- References: {200804161944.m3GJiR5d023112-at-ns.microscopy.com} 17, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 17, 28 -- Content-type: text/plain; charset=US-ASCII 17, 28 -- Content-transfer-encoding: 7BIT 17, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
Dear Isabel, When we got a new water re-circulator a few years ago, they told me that, if it got some algae in it, empty all the water out, fill it with tap water, run it around for a while, then empty the tap water out and replace with distilled water. The tap water in most cities now has enough chlorine in it to kill the algae and flush it out, but they want the re-circulator run routinely with distilled. The other thing I have heard is that just one or two drops of bleach in the tank will kill any organisms without harming any components. Good luck,
-----Original Message----- X-from: isabel.nogueira-at-ist.utl.pt [mailto:isabel.nogueira-at-ist.utl.pt] Sent: April 16, 2008 6:12 AM To: maryflet-at-interchange.ubc.ca
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Email: isabel.nogueira-at-ist.utl.pt Name: Isabel Dias Nogueira
Organization: ICEMS/IST (Lisbon-Portugal)
Title-Subject: [Filtered] SEM and TEM: algae in chiller
Question: Dear fellow microscopists,
We have a conventional SEM, a FEG-SEM (the most recent acquisition) and a TEM, all with water circulation provided by the same chiller. Lately we have noticed some algae forming inside the hose of the FEG-SEM, which is a bit more transparent than the rest. We have changed the water in the chiller, but with no results. My questions are: 1) Is it possible to use some product to remove these algae without damaging any part of the microscopes? If not, what can we do? 2) Do you use destilled water in such chillers?
Thank you for sharing any experience you might have on this subject, Isabel Dias Nogueira
I would keep all Cl out of the system. If any remains, it will corrode the cheap brass most systems use. This brass is high in Zn and is much more susceptible to corrosion than Imperial brass (but it cost $1 more). For my Haskris chiller, I run 10% Ethylene gylcol with distilled water. I try to find the purest DW I can. I've taken selected drops from different DW jugs and put on a clean microscope slide. Let dry and see what remains. Most will have residue. The one that has the least or none is the one to use.
Zeiss chose clear hoses for my SEM but I see no problem since using ethylene glycol. Be sure to change chiller filter(s) about twice a year. Of course, YMMV.
gary g.
At 03:49 PM 4/16/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Email: saubut-at-nrcan.gc.ca Name: Sarah Aubut
Organization: Natural Resources Canada
Title-Subject: [Filtered] SEM camera
Question: Hello,
I work with an Hitachi S-570 SEM that is currently setup with a Polaroid film holder, and was previously setup with a 35mm film camera. I am wondering how easily a digital camera could be substituted for the 35mm film camera. If possible, it would be much more affordable then refitting the microscope with a digital system.
I would appreciate any advice on the subject!!
Thank You! Sarah Aubut Cell Biologist Great Lakes Forestry Centre Sault Ste Marie, ON
The Polaroid back or the 35mm option are still film. They capture line scans as the image is displayed in slow scan on the capture back/port. The problem is that digital cameras are one shot devices--they take a pix of what is there. As such, they do not integrate an image. The final image is not on the record CRT.
I can't think of any way to do this digitally unless using a passive or active digital capture system. A digital camera is not going to work, IMO. However, if you find a workable solution, please let us know.
What is missing is a frame buffer that would store all line scans and present them as a final (total) image as TV which could be frame grabbed as 640x480. But a good record CRT is good for about 4000x3000 lines/inch.
gary g.
At 05:16 PM 4/16/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Wed Apr 16 19:53:14 2008 7, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m3H0rE9o012070 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 16 Apr 2008 19:53:14 -0500 7, 20 -- Message-Id: {200804170053.m3H0rE9o012070-at-ns.microscopy.com} 7, 20 -- Received: (qmail 29879 invoked from network); 16 Apr 2008 17:53:13 -0700 7, 20 -- Received: by simscan 1.1.0 ppid: 29864, pid: 29866, t: 0.1089s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by smtp2 with SMTP; 16 Apr 2008 17:53:13 -0700 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Wed, 16 Apr 2008 17:53:13 -0700 7, 20 -- To: saubut-at-nrcan.gc.ca 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: SEM camera 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200804170016.m3H0GWem028640-at-ns.microscopy.com} 7, 20 -- References: {200804170016.m3H0GWem028640-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-44D73C87 ==============================End of - Headers==============================
It IS possible to fit a digital camera onto an older SEM. I used a Nikon D70S, focussed onto the recording CRT of our Hitachi S-2460N and I took some nice images with the digital camera in the "bulb" mode. That's the key: you must have a digital camera that will "open the shutter" for the entire exposure and close it afterwards. Not many digital cameras have this feature.
In operation, I find the image of interest, open the digital camera shutter with a wireless remote trigger, start the SEM slow scan exposure, wait until the SEM slow scan is over, close the digital camera shutter using the wireless remote. These images are as good (noise-wise) as one would capture with conventional film. The resolution is not as good a film, of course. Yes, there are a LOT of tradeoffs. It is an inconvenience to operate in this mode--unless you are used to using film. Then, it's nearly the same as shooting conventional film. Once the microscopy is complete, remove the memory card from the camera and download them to your computer. One advantage of this method is that the mag bar generated by the SEM will be recorded on the digital image (unlike digital capture systems that insert their own). The cost of this system is basically the cost of the digital camera and modification of the existing film camera holder to accept the digital cam ($1,200 total, in our case).
Having said this, I must confess that on our other microscope, also an S570, we retrofitted a 4pi digital capture system that we are quite pleased with. On the 2460, that I described above, we only rarely need digital images greater than 1 MB. So, we use the X-ray analysis system to capture images. When we need higher resolution digitals, then we could use the Nikon camera.
BTW, an article describing the use of a Canon digital camera to capture images was published in Microscopy Today several years ago. You might check the archives.....or perhaps the author could give us the citation. I apologize for not remembering the author of this very useful technical tip..... aging gray matter, I suppose.
JB
} } Question: Hello, } } I work with an Hitachi S-570 SEM that is currently setup with a } Polaroid film holder, and was previously setup with a 35mm film } camera. I am wondering how easily a digital camera could be } substituted for the 35mm film camera. If possible, it would be much } more affordable then refitting the microscope with a digital system. } } I would appreciate any advice on the subject!! } } Thank You! } Sarah Aubut } Cell Biologist } Great Lakes Forestry Centre } Sault Ste Marie, ON } } saubut-at-nrcan.gc.ca
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
There are two major considerations when using a digital camera to take slow scan images. (I have a background in astronomy, so I'm very familiar with CCD devices and the inherent difficulties with them. I also tried to set up a digital SLR on a Topcon/ISI ABT-SX40)
First, I'm going to make a couple of assumptions. I am going to assume that the digital camera is a digital SLR, like the Nikon D70. I am also going to assume that it has a 'bulb' feature, and that it is going to be able to be mounted directly, sans lens, to the photographic system on the SEM.
The two major concerns are the alignment of the scan and the size of the chip. The chip is made up of 'buckets' (pixels) which basically count how many photons hit the bucket, and register that as an intensity value on the final photo. These buckets are arranged in a nice x-y pattern. If the scan of the microscope is such that the scan line overlaps two rows, or is at an angle to the x-y orientation of the CCD pixels, you can get a bleed-over effect that causes some distortion in your final image. The Nikon D70 has a pixel size of 8x8 microns, and a resolution of 3000x2000 pixels, so it might not be as obvious an artifact at first, but once you start zooming in, you will see it. This can be corrected, though, given some careful alignment when setting it up.
The second major concern is the size of the chip. A 35mm film negative will record an image in a 36 x 24mm area. The CCD chip is physically smaller, recording an image in a space of 24 x 16mm (For a Nikon D70- you can get this measurement off of the specs on your camera). This being said, when you view your images, you have to take into account that you are either not going to get the full image- if you are photographing without any compensating lenses for size, for instance, you might lose the micron bar and information lines at the bottom of the image, for example, or you are going to be off on your measurement calibration if you do correct the optics to capture the full image.
Of minor concern is pixel saturation. When the shutter is open for a longer period of time, and a line dwells in a spot for longer than a microsecond, you have the potential of maxing out the intensity resolution of the pixel. This was a problem that we ran into with using CCD cameras on telescopes- a nearby star would be bright enough to "Fill the buckets" of the CCD pixel, which would then overflow into neighboring pixels. CCDs for the most part are more sensitive to light than film, so make sure that the intensity of the photo CRT is set at the lowest possible value to register a satisfactory image, and you won't max out the intensity on the CCD.
I hope this helps. I know I seem to go off on the D70, but it's the camera I have, and therefore have the booklet with the specs sitting in front of me.
--Justin A. Kraft On Wed, Apr 16, 2008 at 8:19 PM, {saubut-at-nrcan.gc.ca} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both saubut-at-nrcan.gc.ca as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: saubut-at-nrcan.gc.ca } Name: Sarah Aubut } } Organization: Natural Resources Canada } } Title-Subject: [Filtered] SEM camera } } Question: Hello, } } I work with an Hitachi S-570 SEM that is currently setup with a Polaroid film holder, and was previously setup with a 35mm film camera. I am wondering how easily a digital camera could be substituted for the 35mm film camera. If possible, it would be much more affordable then refitting the microscope with a digital system. } } I would appreciate any advice on the subject!! } } Thank You! } Sarah Aubut } Cell Biologist } Great Lakes Forestry Centre } Sault Ste Marie, ON } } saubut-at-nrcan.gc.ca } } Login Host: 198.103.92.143 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 11 -- From zaluzec-at-microscopy.com Wed Apr 16 19:15:11 2008 } 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3H0F9Y1025812 } 10, 11 -- for {microscopy-at-microscopy.com} ; Wed, 16 Apr 2008 19:15:10 -0500 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240801c42c46f9b2db-at-[206.69.208.22]} } 10, 11 -- Date: Wed, 16 Apr 2008 19:15:08 -0500 } 10, 11 -- To: microscopy-at-microscopy.com } 10, 11 -- From: saubut-at-nrcan.gc.ca (by way of MicroscopyListserver) } 10, 11 -- Subject: viaWWW: SEM camera } 10, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
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To protect our chillers from algal growth and freezing ( chiller lives outside) we run them with the heat transfer fluid Hexid A4 from applied thermal control. http://www.app-therm.com/product_hexid.asp. Its pricey but we have no problems with algal growth.
I have no commercial interest, just a satisfied customer.
A.Christine.Richardson Laboratory Manager University of Durham School of Biological & Biomedical Science Centre for Molecular Imaging Science site South Rd Durham England DH1 3LE Tel: 0191 3341285\3341321 Fax: 0191 3341201 E-mail: microscopy.unit-at-dur.ac.uk
==============================Original Headers============================== 9, 29 -- From a.c.richardson-at-durham.ac.uk Thu Apr 17 01:50:18 2008 9, 29 -- Received: from hermes1.dur.ac.uk (hermes1.dur.ac.uk [129.234.8.20]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3H6oHQ2016273 9, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 01:50:17 -0500 9, 29 -- Received: from smtphost4.dur.ac.uk (smtphost4.dur.ac.uk [129.234.4.18]) 9, 29 -- by hermes1.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m3H6o4Pg026100; 9, 29 -- Thu, 17 Apr 2008 07:50:08 +0100 9, 29 -- Received: from weaver.dur.ac.uk (weaver.dur.ac.uk [129.234.4.167]) 9, 29 -- by smtphost4.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m3H6nw46031622; 9, 29 -- Thu, 17 Apr 2008 07:49:58 +0100 9, 29 -- Received: (from httpd-at-localhost) 9, 29 -- by weaver.dur.ac.uk (8.11.7p3+Sun/8.11.1) id m3H6nvo27441; 9, 29 -- Thu, 17 Apr 2008 07:49:57 +0100 (BST) 9, 29 -- X-Authentication-Warning: weaver.dur.ac.uk: httpd set sender to dbl0acr-at-smtphost.dur.ac.uk using -f 9, 29 -- Received: from host86-137-33-210.range86-137.btcentralplus.com (host86-137-33-210.range86-137.btcentralplus.com [86.137.33.210]) 9, 29 -- by webmailimpa.dur.ac.uk (IMP) with HTTP 9, 29 -- for {dbl0acr-at-imaphost.dur.ac.uk} ; Thu, 17 Apr 2008 07:49:57 +0100 9, 29 -- Message-ID: {1208414997.4806f315acdcc-at-webmailimpa.dur.ac.uk} 9, 29 -- Date: Thu, 17 Apr 2008 07:49:57 +0100 9, 29 -- From: a.c.richardson-at-durham.ac.uk 9, 29 -- To: Microscopy-at-microscopy.com 9, 29 -- Cc: isabel.nogueira-at-ist.utl.pt 9, 29 -- MIME-Version: 1.0 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- User-Agent: Internet Messaging Program (IMP) 3.2.1 9, 29 -- X-Originating-IP: 86.137.33.210 9, 29 -- X-MailScanner-ID: m3H6nw46031622 9, 29 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
To protect our chillers from algal growth and freezing ( chiller lives outside) we run them with the heat transfer fluid Hexid A4 from applied thermal control. http://www.app-therm.com/product_hexid.asp. Its pricey but we have no problems with algal growth.
I have no commercial interest, just a satisfied customer.
A.Christine.Richardson Laboratory Manager University of Durham School of Biological & Biomedical Science Centre for Molecular Imaging Science site South Rd Durham England DH1 3LE Tel: 0191 3341285\3341321 Fax: 0191 3341201 E-mail: microscopy.unit-at-dur.ac.uk
==============================Original Headers============================== 5, 30 -- From a.c.richardson-at-durham.ac.uk Thu Apr 17 01:58:09 2008 5, 30 -- Received: from hermes1.dur.ac.uk (hermes1.dur.ac.uk [129.234.8.20]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3H6w8bq027656 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 01:58:08 -0500 5, 30 -- Received: from smtphost3.dur.ac.uk (smtphost3.dur.ac.uk [129.234.4.55]) 5, 30 -- by hermes1.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m3H6vtRL026566; 5, 30 -- Thu, 17 Apr 2008 07:57:59 +0100 5, 30 -- Received: from weaver.dur.ac.uk (weaver.dur.ac.uk [129.234.4.167]) 5, 30 -- by smtphost3.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m3H6vlWB022344; 5, 30 -- Thu, 17 Apr 2008 07:57:47 +0100 5, 30 -- Received: (from httpd-at-localhost) 5, 30 -- by weaver.dur.ac.uk (8.11.7p3+Sun/8.11.1) id m3H6vlH27679; 5, 30 -- Thu, 17 Apr 2008 07:57:47 +0100 (BST) 5, 30 -- X-Authentication-Warning: weaver.dur.ac.uk: httpd set sender to dbl0acr-at-smtphost.dur.ac.uk using -f 5, 30 -- Received: from host86-137-33-210.range86-137.btcentralplus.com (host86-137-33-210.range86-137.btcentralplus.com [86.137.33.210]) 5, 30 -- by webmailimpa.dur.ac.uk (IMP) with HTTP 5, 30 -- for {dbl0acr-at-imaphost.dur.ac.uk} ; Thu, 17 Apr 2008 07:57:47 +0100 5, 30 -- Message-ID: {1208415467.4806f4eb499b3-at-webmailimpa.dur.ac.uk} 5, 30 -- Date: Thu, 17 Apr 2008 07:57:47 +0100 5, 30 -- From: a.c.richardson-at-durham.ac.uk 5, 30 -- To: Microscopy-at-microscopy.com 5, 30 -- Cc: isabel.nogueira-at-ist.utl.pt 5, 30 -- Subject: Algae in water chillers 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.1 5, 30 -- X-Originating-IP: 86.137.33.210 5, 30 -- X-MailScanner-ID: m3H6vlWB022344 5, 30 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
Coming next month is a big experiment in which I'll have to embed 48 specimen in Epon each week during 3 months. Usually I do the embedding of my specimen, cut and analyse them before starting new embedding. So many embeddings in a row is quite unusual for me. My question is about the management of Epon preparation. I'll make 2 runs of 24 samples per week and would not like to prepare new Epon each week. I suppose it is no big deal to leave fresh Epon 3-4 days at 4°C until the next run, but longer? I wonder what would be wise? Preparing a big stock and freezing aliquots? When I bring EPON from RT to 4°C, is there no risk that water condense? Should I cover the surface with Parafilm? Any useful opinion here?
Stephane
PS: suggestion of automatic embedding machines are NOT welcome. The option of microwave-embedding to increase the turnover has not been retained by my boss :-(
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==============================Original Headers============================== 7, 21 -- From nizets2-at-yahoo.com Thu Apr 17 06:19:35 2008 7, 21 -- Received: from web37401.mail.mud.yahoo.com (web37401.mail.mud.yahoo.com [209.191.91.133]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m3HBJZl0022410 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 06:19:35 -0500 7, 21 -- Received: (qmail 53878 invoked by uid 60001); 17 Apr 2008 11:19:32 -0000 7, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 21 -- s=s1024; d=yahoo.com; 7, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 21 -- b=wz5S8caUZZXgz48cakMrm45G1TtL+YVOGfH68DUhs9NukXeb7TSqa0yFkJkJBCnu+IklDmGqs6SuQ4fZk2q5UZfzpByUzOpo+pNqoj8c/SHwF/qgdHGxTlWo7gJLMh0QXtbkTtcSDQJJHF1wqoJYYuWqbrwaRFl6HL3lHntf620=; 7, 21 -- X-YMail-OSG: Bbwb_QcVM1m7bZ5DCFfgxnbGGvm3Xs0X_nATnldom2FvWDY77ZunNyMLQysVpPBpuAIiGHC050SS1KilHfls6RdGNVXFlAZ.XdmGcJYZ.eWCjXhjFP6jy7y8G34- 7, 21 -- Received: from [80.122.101.100] by web37401.mail.mud.yahoo.com via HTTP; Thu, 17 Apr 2008 04:19:32 PDT 7, 21 -- X-Mailer: YahooMailRC/902.40 YahooMailWebService/0.7.185 7, 21 -- Date: Thu, 17 Apr 2008 04:19:32 -0700 (PDT) 7, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 21 -- Subject: Plenty of Epon to manage 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=iso-8859-1 7, 21 -- Message-ID: {636513.53512.qm-at-web37401.mail.mud.yahoo.com} 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3HBJZl0022410 ==============================End of - Headers==============================
I remember well that acetonitrile has been advised as a substitute for PO between ethanol dehydration and Epon embedding. I have always used ethanol and then PO until now but I am eager at using AN instead. Now I wonder if I could not use AN for dehydration too, so it could be used all along! For dehydration factors like penetration time, lipid extraction and hardening of the tissue are important to take into account but having no experience with AN I have no idea. Do some of you use AN for dehydration and Epon embedding? Are there differences between ethanol and AN dehydration (time, hardness,...) to take into account? Also, I have to split the whole procedure into 2 days, usually I stopped in ethanol 70%. Where should I stop until next day with AN?
Best regards, Stephane
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I routinely follow one of the options you listed: "Preparing a big stock and freezing aliquots?"
I mix up a big batch of my epoxy resin (I used Embed812 with hardeners NSA & NMA) and suck it up into disposable 60 cc plastic syringes with catheter tip. I cap the catheter tip with a 1.5 ml Eppendorf microcentrifuge tube which is then wrapped with Parafilm. This will keep moisture out during freeze storage and during warm-up before use. If not all resin is used from syringe, it can be easily re-sealed and placed back into the freezer. And there is never an air space carrying humidity inside the syringe.
NOTE: I do NOT mix in the accelerator into the big batch (I use BDMA). After warming up a syringe of resin (takes about 20 minutes) I then measure out the amount I need and add the BDMA and mix it in just before use. This way you assure that you always start out with minimum viscosity of resin. If you add the accelerator to the bulk mixture and freeze store, the viscosity can increase significantly, even over 24 hours in freezer, much more over months of storage. Without the accelerator added, no increase in viscosity will occur over many months of storage in the freezer.
During the day of use, I do store my disposable beaker of resin mixed with accelerator in the fridge or freezer, sealed with Parafilm, between changes of resin just to slow down the polymerization rate. Or sometimes I leave the syringe of unaccelerated resin out at room temp during the day and just mix in accelerator before each change of resin. Unaccelerated resin will not increase in viscosity at all during the day at room temperature. In fact, it takes a few months to see any increase of viscosity in unaccelerated resin left in a sealed vial at room temp. Try that test with your first batch and see how it looks after 3 months that you will do this big experiment.
Good luck!
Gib
PS (Tissue processor.....tissue processor.....) ;} ) -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America To
} My question is about the management of Epon } preparation. I'll make 2 runs of 24 samples } per week and would not like to prepare new } Epon each week. I suppose it is no big deal } to leave fresh Epon 3-4 days at 4°C until the } next run, but longer? I wonder what would be } wise? Preparing a big stock and freezing } aliquots? When I bring EPON from RT to 4°C, } is there no risk that water condense? Should } I cover the surface with Parafilm? Any useful } opinion here? } } Stephane } } PS: suggestion of automatic embedding } machines are NOT welcome. The option of } microwave-embedding to increase the turnover } has not been retained by my boss :-(
To restrict the growth of algae in the fluid reservoir, I recommend the reservoir cover be kept in place and that all recirculation lines be opaque. This will eliminate the entrance of light, which is required for the growth of most algae.
The use of Chloramine-T, 1 gram per 3.5 liters is recommended. Other algicides can be harmful to the unit's internal components. We use a Thermo recirculating chiller for our Hitachi TEM. Thermo recommends distilled/deionized water with 0.05 to 0.1 megohm-cm reading. Highly distilled/deionized water, above the 3 megohm-cm region, may become aggressive and is not recommended for use with units with wetted parts other than stainless steel. distilled/deionized water in the 15 megohm-cm region is definitely aggressive and should not be used. Do not use a Deionization (DI) filter withinhibited EG. A DI filter will remove inhibitorsfrom the solution rendering the fluid ineffective against corrosion prevention. Also inhibitors increase fluid conductivity.
Proper reservoir cleaning is necessary. Make a monthly visual inspection of the reservoir after initial installation. After several months, the frequency of cleaning will be established. Use a soft cloth for cleaning; do not use steel wool or other abrasive materials. They can scratch the steel surface and initiate rusting.
Best wishes,
Er. IPS Gill
-- Room No. 37, Electron Microscopy & Nanoscience Laboratory, PUNJAB AGRICULTURAL UNIVERSITY LUDHIANA 141 004 INDIA Tel: (+91) 161 2401960-79 Ext. 288 Web: www.pau.edu/emnl Mobile: +91-987-877-2577 email: ipgill-at-gmail.com
On 16/04/2008, isabel.nogueira-at-ist.utl.pt {isabel.nogueira-at-ist.utl.pt} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both isabel.nogueira-at-ist.utl.pt as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: isabel.nogueira-at-ist.utl.pt } Name: Isabel Dias Nogueira } } Organization: ICEMS/IST (Lisbon-Portugal) } } Title-Subject: [Filtered] SEM and TEM: algae in chiller } } Question: Dear fellow microscopists, } } We have a conventional SEM, a FEG-SEM (the most recent acquisition) and a } TEM, all with water circulation provided by the same chiller. } Lately we have noticed some algae forming inside the hose of the FEG-SEM, } which is a bit more transparent than the rest. } We have changed the water in the chiller, but with no results. } My questions are: } 1) Is it possible to use some product to remove these algae without damaging } any part of the microscopes? If not, what can we do? } 2) Do you use destilled water in such chillers? } } Thank you for sharing any experience you might have on this subject, } Isabel Dias Nogueira } } Login Host: 193.136.128.7 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Wed Apr 16 08:01:47 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m3GD1j1x012004 } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 16 Apr 2008 08:01:47 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c42ba91a102b-at-[206.69.208.22]} } 8, 11 -- Date: Wed, 16 Apr 2008 08:01:44 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: isabel.nogueira-at-ist.utl.pt (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: SEM and TEM: algae in chiller } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
-- Room No. 37, Electron Microscopy & Nanoscience Laboratory, PUNJAB AGRICULTURAL UNIVERSITY LUDHIANA 141 004 INDIA Tel: (+91) 161 2401960-79 Ext. 288 Web: www.pau.edu/emnl Mobile: +91-987-877-2577
These are important concerns. Yes, I used a digital SLR with a Nikon Micro Nikkor lens that was focussed on the recording monitor. When recording the image, I adjusted gamma so that the image was rather flat in appearance (and then did a final adjustment in Photoshop). I included as much of the photo-CRT as possible (including the mag scale) and only had to minimally crop the final captured image to exclude areas outside of the photo-CRT.
I am very pleased with the results, but realize that it is somewhat inconvenient (maybe not even practical) if you have multiple or infreqent users who may not remember all the steps.
John
} } --Justin A. Kraft } On Wed, Apr 16, 2008 at 8:19 PM, {saubut-at-nrcan.gc.ca} wrote:
} There are two major considerations when using a digital camera to take } slow scan images. (I have a background in astronomy, so I'm very } familiar with CCD devices and the inherent difficulties with them. I } also tried to set up a digital SLR on a Topcon/ISI ABT-SX40) } } First, I'm going to make a couple of assumptions. I am going to } assume that the digital camera is a digital SLR, like the Nikon D70. } I am also going to assume that it has a 'bulb' feature, and that it is } going to be able to be mounted directly, sans lens, to the } photographic system on the SEM. } } The two major concerns are the alignment of the scan and the size of } the chip. The chip is made up of 'buckets' (pixels) which basically } count how many photons hit the bucket, and register that as an } intensity value on the final photo. These buckets are arranged in a } nice x-y pattern. If the scan of the microscope is such that the scan } line overlaps two rows, or is at an angle to the x-y orientation of } the CCD pixels, you can get a bleed-over effect that causes some } distortion in your final image. The Nikon D70 has a pixel size of 8x8 } microns, and a resolution of 3000x2000 pixels, so it might not be as } obvious an artifact at first, but once you start zooming in, you will } see it. This can be corrected, though, given some careful alignment } when setting it up. } } The second major concern is the size of the chip. A 35mm film } negative will record an image in a 36 x 24mm area. The CCD chip is } physically smaller, recording an image in a space of 24 x 16mm (For a } Nikon D70- you can get this measurement off of the specs on your } camera). This being said, when you view your images, you have to take } into account that you are either not going to get the full image- if } you are photographing without any compensating lenses for size, for } instance, you might lose the micron bar and information lines at the } bottom of the image, for example, or you are going to be off on your } measurement calibration if you do correct the optics to capture the } full image. } } Of minor concern is pixel saturation. When the shutter is open for a } longer period of time, and a line dwells in a spot for longer than a } microsecond, you have the potential of maxing out the intensity } resolution of the pixel. This was a problem that we ran into with } using CCD cameras on telescopes- a nearby star would be bright enough } to "Fill the buckets" of the CCD pixel, which would then overflow into } neighboring pixels. CCDs for the most part are more sensitive to } light than film, so make sure that the intensity of the photo CRT is } set at the lowest possible value to register a satisfactory image, and } you won't max out the intensity on the CCD. } } I hope this helps. I know I seem to go off on the D70, but it's the } camera I have, and therefore have the booklet with the specs sitting } in front of me. } } --Justin A. Kraft } On Wed, Apr 16, 2008 at 8:19 PM, {saubut-at-nrcan.gc.ca} wrote:
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Any hints at getting this tough "bug" embedded in resin for TEM? I've tried very long and gradual dehydration and infiltration with varying resins, solvents. Tried a microwave technique as well. They are all unstable under the beam and tend to pull away.
dot -at- UCHSC in Denver
==============================Original Headers============================== 2, 17 -- From dorothy.dill-at-uchsc.edu Thu Apr 17 16:57:47 2008 2, 17 -- Received: from mailout3.uchsc.edu (mailout3.UCHSC.EDU [140.226.190.222]) 2, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3HLvlP1025877 2, 17 -- for {microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 16:57:47 -0500 2, 17 -- Received: from [140.226.41.77] (dhcp-41-77.UCHSC.EDU [140.226.41.77]) 2, 17 -- by mailout3.uchsc.edu (8.13.8/8.13.8) with ESMTP id m3HDrgDB000730 2, 17 -- for {microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 07:53:44 -0600 2, 17 -- (envelope-from dorothy.dill-at-uchsc.edu) 2, 17 -- Mime-Version: 1.0 (Apple Message framework v753) 2, 17 -- Content-Transfer-Encoding: 7bit 2, 17 -- Message-Id: {6AD06DC3-F199-4DA8-BCFC-54E9E39F37B8-at-uchsc.edu} 2, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 2, 17 -- To: microscopy-at-microscopy.com 2, 17 -- From: Dorothy Dill {dorothy.dill-at-uchsc.edu} 2, 17 -- Subject: Resin embedding of M.Tuberculosis 2, 17 -- Date: Thu, 17 Apr 2008 15:55:13 -0600 2, 17 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
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I am expecting a nasal cilia biopsy next week. The clinician phoned and asked if he could do a brush biopsy (with a cytology brush) to collect the cilia, as opposed to an excisional tissue biopsy. I've never heard of a brush biopsy for cilia - are any of you doing EM on nasal cilia collected with a cytology brush? How do you orient the cilia? If I may ask, what is your processing protocol? (I did tell the clinician no, he would have to take the standard tissue biopsy, but I am interested in learning about this brush biopsy technique.) Thanks in advance for your tips & tricks & info. Rita Kenner Marshfield Clinic
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Email: ipgill-at-gmail.com Name: ER. IPS GILL
Organization: Electron Microscopy & Nanoscience Laboratory
Title-Subject: [Filtered] Re: algae SEM and TEM chiller
Question: Dear Dr.Isabel D. Nogueira,
To restrict the growth of algae in the fluid reservoir, I recommend the reservoir cover be kept in place and that all recirculation lines be opaque. This will eliminate the entrance of light, which is required for the growth of most algae.
The use of Chloramine-T, 1 gram per 3.5 liters is recommended. Other algicides can be harmful to the unit's internal components. We use a Thermo recirculating chiller for our Hitachi TEM. Thermo recommends distilled/deionized water with 0.05 to 0.1 megohm-cm reading. Highly distilled/deionized water, above the 3 megohm-cm region, may become aggressive and is not recommended for use with units with wetted parts other than stainless steel. distilled/deionized water in the 15 megohm-cm region is definitely aggressive and should not be used. Do not use a Deionization (DI) filter withinhibited EG. A DI filter will remove inhibitorsfrom the solution rendering the fluid ineffective against corrosion prevention. Also inhibitors increase fluid conductivity.
Proper reservoir cleaning is necessary. Make a monthly visual inspection of the reservoir after initial installation. After several months, the frequency of cleaning will be established. Use a soft cloth for cleaning; do not use steel wool or other abrasive materials. They can scratch the steel surface and initiate rusting.
Best wishes,
Er. IPS Gill
-- Room No. 37, Electron Microscopy & Nanoscience Laboratory, PUNJAB AGRICULTURAL UNIVERSITY LUDHIANAÝÝ 141 004ÝÝÝÝÝÝ INDIA Tel: (+91) 161 2401960-79ÝÝÝÝÝÝÝÝ Ext. 288 Web:ÝÝ www.pau.edu/emnl Mobile: +91-987-877-2577 email: ipgill-at-gmail.com
Today I have the impression that our EDX detector coupled to our Tecnai G20 TEM microscope is just going crazy. The Dead Time (DT) is constantly on 100% when I want to analyse a particle (objective aperture out). I use classical copper grids and I took care not to be near a grid bar while reading. Here is what I did to extend the diagnostic:
1) When I position the beam on a grid bar, the CPS (counts per second) go up to 300 000 and DT 100%, as expected 2) When I target the center of a grid hole with the objective aperture out, I get a count of 30 and DT 100% 3) When I take the specimen holder out and insert the objective aperture, I have a CPS of 5 and a DT of 100% 4) When I take the specimen holder out and take the objective aperture out, I have a CPS of 0 and a DT of 0%, but sometimes it goes suddenly to 100% then come back to 0% (frequency, about every 2 sec) 5) I can retract the detector, in this case I get the same result of point 4)
I am a biologist, so I cannot understand much of what is happening, but for me it look like the processor is down. What I wonder is why the counts look correct while the DT is crazy. How can I have a CPS of 5 and a DT of 100%???
I never let the dewar warm up (but there is a protection in this case) and the only thing I did recently is calibrate the instrument (last week).
The instrument is from EDAX. I contacted them 2 weeks ago to ask for instructions on how to calibrate it, but still got no answer. So I don't expect much help from their side.
Stephane
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Email: emer.ryan-at-dit.ie Name: Emer
Organization: CREST DIT
Title-Subject: [Filtered] sputter coating
Question: Dear all, a question regarding applying conductive coatings. We're in the process of procuring 2 new FESEMs and I need to update our sample prep equipment. We have an Edwards Auto 306 and a vintage (workhorse)Poloran E5000. I am aware that we need to update to a new sputter coater with e.g. Pt/Pd targets; however it has been suggested to me that we use the Edwards Evaporator for all our coatings needs. I don't think that this will suffice - but apart from the evaporator being slower, can anyone help me build a list of reasons why we need a new sputter coater? Input greatly appreciated.
Set up your normal operating conditions, eg, objective aperture out, etc. then, turn off the e-beam. If you still get 100% deadtime and various count rates, it could mean that your detector FET, attached to the SI(Li) crystal, is blown.
If deadtime and counts go to zero, check condensor aperture, could someone have pulled out the condenser aperture? That would result in very high beam currents down the column perhaps resulting in enough counts to paralyze your system in the test conditions you presented.
As for EDAX, don't give up so soon, contact Steve Mann there, by phone. He's been quite responsive to issues I've had on my EDS systems in the past.
Or, give it a rest overnight. Then try again. Possible software glitch.....?
Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America To
I have been operating an EMS575X Turbo Sputter coater for nearly two years. Besides the fact that it is fast as you already knew, the samples rotate so all sides get an even coat and the machine is built to be compatible with a "Film Thickness Monitor" (FTM) which we chose as an option. This has proven to be a great help and a money saving component in that the Sputter Coater now stops sputtering automatically when the desired coating thickness is obtained - no guessing or timing! Less electricity is used per run and a pre-determined amount of target is used for each run (thickness easily changed for different types of samples) eliminating wasted expensive metal.
The negative was the additional cost of the FTM.
The above is my personal opinion and I have no financial interest etc., just a happy user.
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
} Email: emer.ryan-at-dit.ie } Name: Emer } Organization: CREST DIT } Title-Subject: [Filtered] sputter coating } } Question: Dear all, a question regarding applying conductive coatings. We're } in the process of procuring 2 new FESEMs and I need to update our sample prep } equipment. } We have an Edwards Auto 306 and a vintage (workhorse)Poloran E5000. I am aware } that we need to update to a new sputter coater with e.g. Pt/Pd targets; } however it has been suggested to me that we use the Edwards Evaporator for all } our coatings needs. } I don't think that this will suffice - but apart from the evaporator being } slower, can anyone help me build a list of reasons why we need a new sputter } coater? Input greatly appreciated.
==============================Original Headers============================== 16, 29 -- From connellyps-at-nhlbi.nih.gov Fri Apr 18 12:43:13 2008 16, 29 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IHhCU8025379 16, 29 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 12:43:12 -0500 16, 29 -- X-IronPortListener: Outbound_SMTP 16, 29 -- X-IronPort-AV: E=Sophos;i="4.25,678,1199682000"; 16, 29 -- d="scan'208";a="12073661" 16, 29 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 16, 29 -- by nihxway2out.hub.nih.gov with ESMTP; 18 Apr 2008 13:40:05 -0400 16, 29 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 16, 29 -- Fri, 18 Apr 2008 13:43:04 -0400 16, 29 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 16, 29 -- Fri, 18 Apr 2008 17:43:04 +0000 16, 29 -- User-Agent: Microsoft-Entourage/11.4.0.080122 16, 29 -- Date: Fri, 18 Apr 2008 13:39:43 -0400 16, 29 -- Subject: Re: [Microscopy] viaWWW: sputter coating 16, 29 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 16, 29 -- To: {emer.ryan-at-dit.ie} 16, 29 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 29 -- Message-ID: {C42E551F.1A37%connellyps-at-nhlbi.nih.gov} 16, 29 -- Thread-Topic: [Microscopy] viaWWW: sputter coating 16, 29 -- Thread-Index: Acihey9/bkX/rw1uEd2rNQANk2Yv1A== 16, 29 -- In-Reply-To: {200804181315.m3IDF8Pj027293-at-ns.microscopy.com} 16, 29 -- Mime-version: 1.0 16, 29 -- Content-type: text/plain; 16, 29 -- charset="ISO-8859-1" 16, 29 -- X-OriginalArrivalTime: 18 Apr 2008 17:43:04.0994 (UTC) FILETIME=[A7E57020:01C8A17B] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3IHhCU8025379 ==============================End of - Headers==============================
THE APPLICANTS WILL BE SERVED ON A FIRST IN FIRST OUT BASIS.. SEND ME AN E-MAIL AND COME TO ERICE...
Il giorno 06/apr/08, alle ore 08:57, Diaspro ha scritto:
} Dear All, } there are ONLY 2 more seats available thanks to the generosity of the } Ettore Majorana Center for } } INTERNATIONAL SCHOOL OF BIOPHYSICS «ANTONIO BORSELLINO» 36th Course: } “MULTIDIMENSIONAL OPTICAL FLUORESCENCE } MICROSCOPY TOWARDS NANOSCOPY”, that will be held in ERICE, SICILY, } ITALY on 19- 29 } APRIL 2008. Prof. A. ZICHICHI is the PRESIDENT OF CCSEM AND DIRECTOR } OF THE CENTRE, } and Prof. A. DIASPRO (UNIVERSITY OF GENOA) and Prof. V.TORRE (SISSA, } TRIESTE), are } DIRECTORS OF THE COURSE (http://www.ccsem.infn.it/). Download poster } in the News at www.lambs.it. } } } PURPOSE OF THE COURSE } A bright new future has appeared, as the nano-era has taken and placed } a whole new array of tools in the } hands of biophysicists, who are keen to go deeper into the intricacies } of how biological systems work. } Forever pushing the boundaries, biophysicists are sliding the research } focus from the micro- towards the } nano- and even sub-nano scale. Now, Biophysics is a molecular science, } rapidly moving to the nanoscale, } demanding for an interdisciplinary approach more than in the past, as } in the Antonio Borsellino’s } expectations. It seeks to explain biological function in terms of the } molecular structures and properties of } specific molecules. As part of this effort, some biophysicists are } involved in inventing new methods and } building new instruments for monitoring these structures. Many of the } exciting new developments in } microscopy and more specifically in optical microscopy, in terms of } imaging and manipulation, spectroscopy } and visualization, are a segment and necessity of this trend. The } recent advances to the “nano” level, both as } complement to electron and scanning probe microscopy and as } development of the so-called optical } nanoscopy, witnesses the relevance of the field. } } TOPICS and LECTURERS } In vivo imaging, SHG *; P. BIANCHINI, University of Genoa, IT } Fluorescence Spectroscopy, GFP Photophysics *; R. BIZZARRI, NESTINFM, } SNS, Pisa, IT; } Optics, Confocal Microscopy, THG *; F. BRAKENHOFF, University of } Amsterdam, NL; FRAP, Single } particle tracking *; K. BRAECKMANS, Ghent University, BE; } Single molecule force spectroscopy *; J. BRUJIC, New York University, } USA – EBSA lecturer; } Fluctuation Microscopies for biological tissues G. CHIRICO, University } of Milan-Bicocca, IT; } Micro-particle manipulation *; D. COJOC, TASC, INFM, Trieste, IT; } SHG, CARS, 2PE *; C. COMBS, NIH, Bethesda, USA; } 2PE, 3D imaging *; A. DIASPRO, University of Genoa,IT – EBSA President } Elect, BJ EBM; } Micro/Nano Optical Manipulation *; E. Di FABRIZIO, University of } Catanzaro Magna Graecia, IT; } Raster Image Correlation Spectroscopy, Photon Counting *; M. DIGMAN, } UC Irvine, USA; } High-content screening *; M. FARETTA, IFOM-IEO, Milan, IT; } Correlative Microscopy *; U. FASCIO, University of Milan, IT; } Fluorescence Lifetime, FRET *; H.C. GERRITSEN, Utrecht University, NL; } FCS, Global Data Analysis *; E. GRATTON, UC Irvine, USA – BJ EBM; } Time lapse imaging *; S. GUIDO, University of Naples, IT; } Fluorescente Optical Nanoscopy *; S. HELL, MPI, Goettingen, DE; } Scanning Microscopy, Optical aberrations *; M. MARTINEZ CORRAL, Univ. } of Valencia, ES; } Optical systems, Scanning Microscopy *; F. QUERCIOLI, CNR-ISC, } Florence, IT; } 2PE imaging in vivo *; GIMMI RATTO, Institute of Neuroscience, CNR, } Pisa, IT; } 2PE, Fast scanning methods *; P. SAGGAU, Baylor College of Med. } Houston, Texas,USA; Correlative } Microscopy at cryo-Temperatures *; A. SARTORI, Institut Pasteur, } Paris, FR; } Light Scattering, FCS applications *; P.L. SAN BIAGIO, CNR-IBF, } Palermo, IT – SIBPA President; } Molecular landscapes by means of AFM *; G. SCOLES, Princeton } University, USA; } Linear and Non linear Optical Microscopy *; C. SHEPPARD, Ntl Univ. of } Singapore, Singapore; } Laser Light Sheet Fluorescence Microscopy *; P. KELLER, EMBL, } Heidelberg, DE; } Laser scissors and tweezers in cell biology *; I. TOLIC-NORRELYKKE, } MPI, Dresden, DE – SIBPA Lecturer; } Fluorescence imaging in Neuroscience *; V. TORRE, SISSA, Trieste, IT; } Quantitative colocalization *; C. USAI, CNR-IBF, Genoa, IT; } Confocal Microscopy, Structured light methods *; T. WILSON, University } of Oxford, UK; } Photoswitch-activatable fluorescent proteins, Lifetime *; F.WOUTERS, } Univ. of Goettingen, DE. } } PRACTICAL INFORMATION } } Deadline: April 15th, 2008 } Send an e-mail, asap, to: diaspro-at-fisica.unige.it } } Cost: 1000 Euros, it includes scientific material, lodging and meals } for the whole period (can be paid on site or by bank transfer - ask } for coordinates) and transportation from/to the Palermo/Trapani } airport. } Participants are supposed to arrive in Erice on April 19th, possibly } not later than 5 p.m. Please, provide asap your travel details. } The course starts on April 20th 8:30 a.m. and ends on April 28th , } 2008, 8:00 p.m. } } Lessons scheduling, the Program is built on the topics reported in } this message: 8,30-9,15; 9,30-10,15; break; } 10,45-11,30; 11,45-12,30; LUNCH; 14,45-15,15 Companies and Students } time; 15,30-16,15; 16,30-17,15; } break; 17,45-18,30; 18,45-19,30; 21-23 Free time in the Canteen. There } will be time for Students presentations (10-20 minutes slots) } There will be the possibility of submitting an article for the } Springer related book. } } There are 20 more seats available thanks to the generosity of the } Ettore Majorana Center that will be assigned on a FIFO rule (first in } first out) } } ALL the BEST } ALBY } } } } } ---------------------------------------------------- } "Water slowly flowed under the sky" (Cesare Pavese) } ----------------------------------------------------- } Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, } Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 } Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it } ; } EBSA is Biophysics in Europe - European Biophysical Societies' } Association www.ebsa.org } ---------------------------------------------- } } } } } } } ------------------------------------ } } MPLSM-Users, the Multiphoton Mailing List, } Steve M. 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---------------------------------------------------- "Water slowly flowed under the sky" (Cesare Pavese) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 10, 26 -- From sekkio-at-mac.com Fri Apr 18 13:53:14 2008 10, 26 -- Received: from smtpoutm.mac.com (smtpoutm.mac.com [17.148.16.67]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IIrDmU008395 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 13:53:13 -0500 10, 26 -- Received: from mac.com (asmtp002-s [10.150.69.65]) 10, 26 -- by smtpoutm.mac.com (Xserve/smtpout004/MantshX 4.0) with ESMTP id m3IIrCK5004184; 10, 26 -- Fri, 18 Apr 2008 11:53:12 -0700 (PDT) 10, 26 -- Received: from [10.0.1.2] (host145-127-dynamic.16-79-r.retail.telecomitalia.it [79.16.127.145]) 10, 26 -- (authenticated bits=0) 10, 26 -- by mac.com (Xserve/asmtp002/MantshX 4.0) with ESMTP id m3IIr5sH017140 10, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 10, 26 -- Fri, 18 Apr 2008 11:53:08 -0700 (PDT) 10, 26 -- Cc: microscopy-at-microscopy.com, MicroscopyListServer by {venu-at-purdue.edu} , 10, 26 -- Confocal List Microscopy {CONFOCAL-at-LISTSERV.BUFFALO.EDU} 10, 26 -- Message-Id: {44D24BD9-9A2B-4AD5-B36B-8787F5E1B555-at-mac.com} 10, 26 -- From: Diaspro {sekkio-at-mac.com} 10, 26 -- To: mplsm-users-at-yahoogroups.com 10, 26 -- In-Reply-To: {B7C1942F-467D-497D-A29F-DE6B49FF6563-at-mac.com} 10, 26 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 10, 26 -- Mime-Version: 1.0 (Apple Message framework v919.2) 10, 26 -- Subject: 2 Last minute Seats - School on Multidimensional Microscopy, Erice Sicily 19-29 April 2008 10, 26 -- Date: Fri, 18 Apr 2008 20:53:04 +0200 10, 26 -- References: {B7C1942F-467D-497D-A29F-DE6B49FF6563-at-mac.com} 10, 26 -- X-Mailer: Apple Mail (2.919.2) 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3IIrDmU008395 ==============================End of - Headers==============================
The same CuAl standard produces a normal spectrum on other instruments.
Questions: Have you seen this type of symptom before? Does this spectrum suggest a part of the system that is malfunctioning?
History: we sent the controller to PGT/Bruker for evaluation and their bench test did not produce the split peaks. Their only suggestion now is to send the entire detector. Before doing that, we wanted to do a little troubleshooting here.
Thanks for any suggestions.
--John John Chandler, Ph.D. Manager, Electron Microscopy Lab Colorado School of Mines 1500 Illinois Street Golden, CO 80401-1887 jpchandl-at-mines.edu 303.384.2203 (office) 970.219.5706 (cell)
==============================Original Headers============================== 11, 19 -- From jpchandl-at-mines.edu Fri Apr 18 15:08:00 2008 11, 19 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU [138.67.130.5]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IK80ns024827 11, 19 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 15:08:00 -0500 11, 19 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 11, 19 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id m3IK7urP030401 11, 19 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 14:07:59 -0600 11, 19 -- From: "John Chandler" {jpchandl-at-mines.edu} 11, 19 -- To: {microscopy-at-microscopy.com} 11, 19 -- Subject: EDX: Split peaks on spectra 11, 19 -- Date: Fri, 18 Apr 2008 14:07:57 -0600 11, 19 -- Message-ID: {001d01c8a18f$e4d8d530$ad1c438a-at-mines.edu} 11, 19 -- MIME-Version: 1.0 11, 19 -- Content-Type: text/plain; 11, 19 -- charset="us-ascii" 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- X-Mailer: Microsoft Office Outlook 11 11, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 11, 19 -- Thread-Index: Acihj+SrJoOam56LQBOqpSdM+UMDbg== ==============================End of - Headers==============================
That looks a bit familiar to a failure we had about six years ago. Our Ge detector warmed up and apparently cracked. I am guessing that lead to incomplete charge collection for each photon and gave a shadow effect. You can see an image of one of our problem spectra at ftp://www.marl.iastate.edu/General/ISIS/Ge_detector%20failing/Failing%20 Ge%20detector.gif. I overlaid spectra of Al and Ti.
Our only recourse was to get the crystal replaced. We sent it overseas to Gresham They did good work for us at a fair price but waiting on customs is a hassle.
I also understand Jim Nicolino does similar work with his company PulseTor/AAT 1816 St. Johns Bluff Road Suite 305 Jacksonville, Florida 32246 904.646.3069 FAX 904.646.3131 JNicolino-at-comcast.net We found out about him after we sent the system off to Gresham. I cannot vouch for his work, but I suppose others here can.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: jpchandl-at-mines.edu [mailto:jpchandl-at-mines.edu] Sent: Friday, April 18, 2008 3:09 PM To: wesaia-at-iastate.edu
We are looking for some insights from the list. One of our instruments has the following configuration:
Philips CM200 TEM with scanning attachment PGT EDX: Prism 2000 detector and Avalon controller
We recently started seeing spectra with "split peaks". An example spectrum is at:
The same CuAl standard produces a normal spectrum on other instruments.
Questions: Have you seen this type of symptom before? Does this spectrum suggest a part of the system that is malfunctioning?
History: we sent the controller to PGT/Bruker for evaluation and their bench test did not produce the split peaks. Their only suggestion now is to send the entire detector. Before doing that, we wanted to do a little troubleshooting here.
Thanks for any suggestions.
--John John Chandler, Ph.D. Manager, Electron Microscopy Lab Colorado School of Mines 1500 Illinois Street Golden, CO 80401-1887 jpchandl-at-mines.edu 303.384.2203 (office) 970.219.5706 (cell)
==============================Original Headers============================== 18, 37 -- From wesaia-at-iastate.edu Fri Apr 18 15:33:33 2008 18, 37 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 18, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IKXX2j006047 18, 37 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 18 Apr 2008 15:33:33 -0500 18, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 18, 37 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m3IKXVqW023286; 18, 37 -- Fri, 18 Apr 2008 15:33:31 -0500 18, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 18, 37 -- id 51b2_78526062_0d86_11dd_98c2_00137253420a; 18, 37 -- Fri, 18 Apr 2008 15:31:47 -0500 18, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 18, 37 -- by despam-10.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m3IKXNTY010078; 18, 37 -- Fri, 18 Apr 2008 15:33:24 -0500 18, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 18, 37 -- Fri, 18 Apr 2008 15:33:28 -0500 18, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 37 -- Content-class: urn:content-classes:message 18, 37 -- MIME-Version: 1.0 18, 37 -- Content-Type: text/plain; 18, 37 -- charset="us-ascii" 18, 37 -- Subject: RE: [Microscopy] EDX: Split peaks on spectra 18, 37 -- Date: Fri, 18 Apr 2008 15:34:44 -0500 18, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72702C6668B-at-maire.eng.iastate.edu} 18, 37 -- In-Reply-To: {200804182009.m3IK91eH025964-at-ns.microscopy.com} 18, 37 -- X-MS-Has-Attach: 18, 37 -- X-MS-TNEF-Correlator: 18, 37 -- Thread-Topic: [Microscopy] EDX: Split peaks on spectra 18, 37 -- Thread-Index: AcihkA6SweA2QMeBTUmfzjHzZ8LixgAAjtDg 18, 37 -- References: {200804182009.m3IK91eH025964-at-ns.microscopy.com} 18, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 18, 37 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 18, 37 -- Cc: {jpchandl-at-mines.edu} 18, 37 -- X-OriginalArrivalTime: 18 Apr 2008 20:33:28.0924 (UTC) FILETIME=[75D559C0:01C8A193] 18, 37 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.4.18.132030 18, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_2000_2999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_2_BODY 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0' 18, 37 -- Content-Transfer-Encoding: 8bit 18, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3IKXX2j006047 ==============================End of - Headers==============================
We had someone try this here once. I think we sort of shook and spun the cells off the brush after fixation (immersed the brush in fixative right after collection), then pelleted the cells in agar and then processed as normal. It was a bit on the fussy side, but I think they got sections. They didn't pursue it, so we never perfected the technique! But this was on young children, so regular tissue biopsy was out.
Aloha, Tina
} Email: kenner.rita-at-marshfieldclinic.org } Name: Rita Kenner } } Organization: Marshfield Clinic, Marshfield WI. 715-387-9159 } } Title-Subject: [Filtered] Nasal cilia: brush biopsy } } Question: Greetings all - } } I am expecting a nasal cilia biopsy next week. The clinician phoned and asked if he could do a brush biopsy (with a cytology brush) to collect the cilia, as opposed to an excisional tissue biopsy. I've never heard of a brush biopsy for cilia - are any of you doing EM on nasal cilia collected with a cytology brush? How do you orient the cilia? If I may ask, what is your processing protocol? } (I did tell the clinician no, he would have to take the standard tissue biopsy, but I am interested in learning about this brush biopsy technique.) Thanks in advance for your tips & tricks & info. } Rita Kenner } Marshfield Clinic } } Login Host: 192.236.21.41 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu Apr 17 19:03:02 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3I031lE011095 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 17 Apr 2008 19:03:02 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c42d95a6250f-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 17 Apr 2008 19:02:59 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: kenner.rita-at-marshfieldclinic.org (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Nasal cilia: brush biopsy } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 21 -- From tina-at-pbrc.hawaii.edu Fri Apr 18 15:41:19 2008 6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IKfIPC018795 6, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 18 Apr 2008 15:41:19 -0500 6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m3IKfDdC014887 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 21 -- Fri, 18 Apr 2008 10:41:14 -1000 (HST) 6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m3IKfCDQ014884; 6, 21 -- Fri, 18 Apr 2008 10:41:12 -1000 (HST) 6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 21 -- Date: Fri, 18 Apr 2008 10:41:11 -1000 (HST) 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 21 -- X-Sender: tina-at-halia 6, 21 -- To: kenner.rita-at-marshfieldclinic.org 6, 21 -- cc: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 21 -- Subject: Re: [Microscopy] viaWWW: Nasal cilia: brush biopsy 6, 21 -- In-Reply-To: {200804180004.m3I04eQN014178-at-ns.microscopy.com} 6, 21 -- Message-ID: {Pine.GSO.4.21.0804181037500.14238-100000-at-halia} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Does anybody have a manual (Or any information) regarding an Edwards EQ80f? It appears to be a leak detector of some kind, but I just have the control box, not the detector head. I would like to get some info on the detector head and see what I can do to use this to pinpoint leaks in my SEM.
Thanks,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
I just received a second JSM-35C which I used to get some parts for mine. I have a huge amount of parts left over, though, and I thought I'd offer them to the group. The only parts I don't have are the transformer, the metal skins, and the chassis. Otherwise, I have the lenses and electronics all parted out (Including the HV tank and pump box) and am willing to separate anything you may need.
I also have two EDS detectors, one is a Kevex 3200-0068 and the other is a Tracor Micro-Z detector. I also have a Kevex Delta-III cabinet (with keyboard minus monitor)
I just picked up a Link Analytical eXL system, which is in working order, and I would like a detector that is compatible with it.
Or, if anyone has an extra EDS system that works, that they would be willing to trade, I really want to show the students what elemental analysis is and what we are capable of.
So, if you have anything of interest that you want to trade for parts, let me know. I'm more than happy to package something up.
Thanks,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
I am going to put a disclaimer up front. We manufacture and sell the IBS/e ion sputter coating system.
Now, having said that, consider a sphere sitting on the tilting and rotating holder of your sputter system. Now consider the amount of time each surface element of the sphere is pointed at the sputter target. When your stage is tilted at 90°, if it doesn't stay there longer to account for the amount of time to cover each surface element with the same amount of material as when the stage is at 0°, then the coating over the surface will not be uniform. In other words, the stage should spend less time going through 0° than when it is turning around at 90°.
Further, because you want to coat the sample uniformly, it is not beneficial to coat the sample fast. You want to grow the film slowly so that you have better control over both the film thickness growth and the uniformity of the film by allowing your stage to go over all possible angular ranges over the duration of growth. In our system, I will actually purposely grow some films slower to enhance the uniformity of highly topographic samples.
Magnetron sputter systems are fast, but they have a couple of disadvantages. Magnetron systems can "spit" material sometimes. These are larger particles that can be incorporated into the film. Also, the distribution of the sputtered material changes over time as a function of the wear pattern in the target. This can affect the accuracy of a quartz crystal thin film monitor system.
Don't forget that you also have to run the unit a little before coating to get rid of any surface oxide on the target. Different target materials oxidize differently, so the amount of time that you have to pre-sputter the target is dependent on the material. In practice, what this means is that you have to have a shutter for your sample so that it is not coating the sample while you are conditioning the target.
On the other hand, an ion beam system has a fairly stable deposition pattern over time. The deposition rate has a cosine distribution that doesn't matter too much about how the target is eroded over time. It is slower, which I think is a good thing for high resolution SEM coatings. And it does not "spit".
One other thing that separates the systems. You can't deposit magnetic materials easily with magnetron sputtering. Why is this important? Several investigators are using Ni and Fe thin films to use as catalyst layers for the growth of carbon nanotubes. Another thing is that you can readily change targets with an ion beam. In our system, we can have up to four targets that can be switched while under vacuum. The Gatan system has two targets. What this means is that you can use these systems to put down multi-layer thin film coatings that can be used for calibration samples and standard samples for TEM. I've actually performed the calculations for using a bi-layer thin film with known thicknesses to act as a thin film binary standard for AEM. I just haven't done the experiments yet.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: connellyps-at-nhlbi.nih.gov [mailto:connellyps-at-nhlbi.nih.gov] Sent: Friday, April 18, 2008 10:49 AM To: Walck-at-SouthBayTech.com
Emer,
I have been operating an EMS575X Turbo Sputter coater for nearly two years. Besides the fact that it is fast as you already knew, the samples rotate so all sides get an even coat and the machine is built to be compatible with a "Film Thickness Monitor" (FTM) which we chose as an option. This has proven to be a great help and a money saving component in that the Sputter Coater now stops sputtering automatically when the desired coating thickness is obtained - no guessing or timing! Less electricity is used per run and a pre-determined amount of target is used for each run (thickness easily changed for different types of samples) eliminating wasted expensive metal.
The negative was the additional cost of the FTM.
The above is my personal opinion and I have no financial interest etc., just a happy user.
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
} Email: emer.ryan-at-dit.ie } Name: Emer } Organization: CREST DIT } Title-Subject: [Filtered] sputter coating } } Question: Dear all, a question regarding applying conductive coatings. } We're in the process of procuring 2 new FESEMs and I need to update } our sample prep equipment. } We have an Edwards Auto 306 and a vintage (workhorse)Poloran E5000. I } am aware that we need to update to a new sputter coater with e.g. } Pt/Pd targets; however it has been suggested to me that we use the } Edwards Evaporator for all our coatings needs. } I don't think that this will suffice - but apart from the evaporator } being slower, can anyone help me build a list of reasons why we need a } new sputter coater? Input greatly appreciated.
==============================Original Headers============================== 16, 29 -- From connellyps-at-nhlbi.nih.gov Fri Apr 18 12:43:13 2008 16, 29 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IHhCU8025379 16, 29 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 12:43:12 -0500 16, 29 -- X-IronPortListener: Outbound_SMTP 16, 29 -- X-IronPort-AV: E=Sophos;i="4.25,678,1199682000"; 16, 29 -- d="scan'208";a="12073661" 16, 29 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 16, 29 -- by nihxway2out.hub.nih.gov with ESMTP; 18 Apr 2008 13:40:05 -0400 16, 29 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 16, 29 -- Fri, 18 Apr 2008 13:43:04 -0400 16, 29 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 16, 29 -- Fri, 18 Apr 2008 17:43:04 +0000 16, 29 -- User-Agent: Microsoft-Entourage/11.4.0.080122 16, 29 -- Date: Fri, 18 Apr 2008 13:39:43 -0400 16, 29 -- Subject: Re: [Microscopy] viaWWW: sputter coating 16, 29 -- X-from: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 16, 29 -- To: {emer.ryan-at-dit.ie} 16, 29 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 29 -- Message-ID: {C42E551F.1A37%connellyps-at-nhlbi.nih.gov} 16, 29 -- Thread-Topic: [Microscopy] viaWWW: sputter coating 16, 29 -- Thread-Index: Acihey9/bkX/rw1uEd2rNQANk2Yv1A== 16, 29 -- In-Reply-To: {200804181315.m3IDF8Pj027293-at-ns.microscopy.com} 16, 29 -- Mime-version: 1.0 16, 29 -- Content-type: text/plain; 16, 29 -- charset="ISO-8859-1" 16, 29 -- X-OriginalArrivalTime: 18 Apr 2008 17:43:04.0994 (UTC) FILETIME=[A7E57020:01C8A17B] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3IHhCU8025379 ==============================End of - Headers==============================
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==============================Original Headers============================== 34, 23 -- From walck-at-southbaytech.com Fri Apr 18 16:31:34 2008 34, 23 -- Received: from flpi195.prodigy.net (flpi195.sbcis.sbc.com [207.115.20.197]) 34, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3ILVYno025907 34, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 16:31:34 -0500 34, 23 -- X-ORBL: [99.154.21.201] 34, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 34, 23 -- by flpi195.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id m3ILVSVx003034; 34, 23 -- Fri, 18 Apr 2008 14:31:30 -0700 34, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 34, 23 -- To: {Microscopy-at-microscopy.com} 34, 23 -- Cc: {connellyps-at-nhlbi.nih.gov} 34, 23 -- Subject: RE: [Microscopy] Re: viaWWW: sputter coating -magnetrons versus ion beams. 34, 23 -- Date: Fri, 18 Apr 2008 14:31:37 -0700 34, 23 -- Message-ID: {00ad01c8a19b$982fa040$7801a8c0-at-dynamicbl8uno3} 34, 23 -- MIME-Version: 1.0 34, 23 -- Content-Type: text/plain; 34, 23 -- charset="iso-8859-1" 34, 23 -- X-Mailer: Microsoft Office Outlook 11 34, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 34, 23 -- Thread-Index: AcihfIM+HuKE4X0HR3OOdbGrR4Rx7gABjZ7w 34, 23 -- In-Reply-To: {200804181749.m3IHnCun030771-at-ns.microscopy.com} 34, 23 -- Content-Transfer-Encoding: 8bit 34, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3ILVYno025907 ==============================End of - Headers==============================
I have recently gotten into this work and have had mixed results. A lot of the cells I have looked at are severely damaged, cell debris all over the sample that is held together by mucus (think Boogers!) and the cells are in many different orientations (perfect cross-sections of the cilia wanted of course and I had 3 on my first section and 0 on the second sample sectioned twice which had a few bacteria) . The fixation follows a ciliary motion study so I requested a control to see if there is a difference between freshly fixed cells and those which have been in media for various lengths of time and I am not sure of the temperature during the study. The problem is that they can not take scrapings (do not know about brushes) from healthy children, only sick ones, AND the samples are so small that they do not want to sacrifice half to do a comparison study.
I am going on a fact finding trip next week to UNC where some people there have been doing this type of work for a while now and I hope to learn what they are doing to get decent results. Perhaps someone from that lab will have seen your request for information and answered too.
The fixative that I have been advised to use is cold 2% glutaraldehyde+2% paraformaldehyde+0.5% tannic acid+0.05M Phosphate Buffer (Sorenson's) at pH 7.2 followed by 1% OsO4, no UA, EtOH dehydration Epon embedding.
Good luck! Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
} Hi, Rita- } } We had someone try this here once. I think we sort of shook and spun the } cells off the brush after fixation (immersed the brush in fixative right } after collection), then pelleted the cells in agar and then processed as } normal. It was a bit on the fussy side, but I think they got sections. } They didn't pursue it, so we never perfected the technique! But this was } on young children, so regular tissue biopsy was out. } } Aloha, Tina * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } Email: kenner.rita-at-marshfieldclinic.org } } Name: Rita Kenner } } Organization: Marshfield Clinic, Marshfield WI. 715-387-9159 } } Title-Subject: [Filtered] Nasal cilia: brush biopsy } } } } I am expecting a nasal cilia biopsy next week. The clinician phoned and asked } } if he could do a brush biopsy (with a cytology brush) to collect the cilia, } } as opposed to an excisional tissue biopsy. I've never heard of a brush } } biopsy for cilia - are any of you doing EM on nasal cilia collected with a } } cytology brush? How do you orient the cilia? If I may ask, what is your } } processing protocol? } } (I did tell the clinician no, he would have to take the standard tissue } } biopsy, but I am interested in learning about this brush biopsy technique.) } } Thanks in advance for your tips & tricks & info. } } Rita Kenner } } Marshfield Clinic
==============================Original Headers============================== 9, 28 -- From connellyps-at-nhlbi.nih.gov Fri Apr 18 16:36:41 2008 9, 28 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3ILafCp000864 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 16:36:41 -0500 9, 28 -- X-IronPortListener: Outbound_SMTP 9, 28 -- X-IronPort-AV: E=Sophos;i="4.25,679,1199682000"; 9, 28 -- d="scan'208";a="8973027" 9, 28 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 9, 28 -- by nihxway3out.hub.nih.gov with ESMTP; 18 Apr 2008 17:31:14 -0400 9, 28 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 28 -- Fri, 18 Apr 2008 17:36:36 -0400 9, 28 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 9, 28 -- Fri, 18 Apr 2008 21:36:36 +0000 9, 28 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 28 -- Date: Fri, 18 Apr 2008 17:33:16 -0400 9, 28 -- Subject: Re: [Microscopy] Re: viaWWW: Nasal cilia: brush biopsy 9, 28 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 28 -- Message-ID: {C42E8BDC.1A57%connellyps-at-nhlbi.nih.gov} 9, 28 -- Thread-Topic: [Microscopy] Re: viaWWW: Nasal cilia: brush biopsy 9, 28 -- Thread-Index: Acihm8/lDr1ptw2PEd2rNQANk2Yv1A== 9, 28 -- In-Reply-To: {200804182045.m3IKjbQx026707-at-ns.microscopy.com} 9, 28 -- Mime-version: 1.0 9, 28 -- Content-type: text/plain; 9, 28 -- charset="ISO-8859-1" 9, 28 -- X-OriginalArrivalTime: 18 Apr 2008 21:36:36.0509 (UTC) FILETIME=[4768F4D0:01C8A19C] 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3ILafCp000864 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Problems coating with Denton Vacuum Coater
Question: Hello,
I am a new user of SEM and am having problems getting a coating of platinum with my Denton Vacuum coater (DV-502A). I currently use a Technics Hummer V to coat with Au/Pd, but since it is pretty old I would like to use the Denton with Pt as backup, plus as I understand it Pt is better to use for imaging. I have no problems coating with carbon using the Denton, but whenever I try to coat with Pt, I have trouble. The basket that the current runs through always breaks and instead of getting a nice sputter, I just get a spark. I've tried turning up the current very very slowly and pausing before going each notch and that doesn't seem to work. Can anyone give me any good pointers? Thanks!
We had an older dual beam VCR Group microsputter coater mounted on an Edwards 306A. Both units integrated fine. However, it was a bear to change the Edwards back and forth betwen microsputtering and other thermal evaporations.
1. The Edwards is a fine unit but it is oil pumped. That was not a big problem with our Cr coatings but others wanted a turbopump on it.
2. We had to cover all the feed throughs with a steel bell jar that was more like a collar. The collar was very heavy and had a large diameter. The microsputter coater fit on top of this collar. It took two of us to change back and forth from the microsputter coater mode to regular Edwards' modes.
3. You need lots of bench space for the bell jar, the heavy collar, and the sputter unit. A desktop sputter unit is MUCH smaller, lighter, and takes less bench top space.
4. Cycle times are longer with a large free standing large bell jar evaporator. They have their place and uses. However, then there is the LN2 trap issue and waiting for cooling and pump down with LN2. Then you might need a rotator system.
Do you really want to go there?
I took a 30+ year old vacuum evaporator from another group that was headed to the dumpster and rebuilt it. It was not fun. Some nut opened all the valves and pumped the thing overnight with the DP heater on and the vent valve open. (Don't hire a plumber as a vacuum technician.) The DP chimney had carbon foam in it the next day. That's just one of the 15+ things that were wrong with the evaporator before I got it. It took three months of my spare time to finish the rebuild. Once I got it working, then I had to convert my new used evaporator from a dual thermal boat unit. I installed a Ladd carbon rod and tungsten basket unit in the evaporator. So I got the new smaller evaporator unit working and we seldom had to change the Edwards over again.
What's the moral of the story? That's how much I/we hated converting the Edwards. If you are a weight lifter, you will love converting back and forth. I eventually passed on converting. The dedicated carbon rod evaporator setup I built put a stop to the repeated conversions between carbon to chrome to carbon to Pt, to carbon, etc.
Scott Walck at South Bay Technologies might want to chime in there on the oil and turbopump issue. He saw this unit in operation. SBT makes high resolution coating units.
There are other units available from other manufacturers too.
JMO,
Paul
At 08:05 AM 4/18/08 -0500, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 27 -- From beaurega-at-westol.com Fri Apr 18 17:33:24 2008 14, 27 -- Received: from smtp-gateway-6.winbeam.com (smtp-gateway-6.winbeam.com [64.84.96.16] (may be forged)) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3IMXOk0000570 14, 27 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 17:33:24 -0500 14, 27 -- X-Winbeam-MailScanner-Watermark: 1209162654.89053-at-Ren78967hVQyU9L8WAWFCg 14, 27 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 14, 27 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id m3IMUchG026293 14, 27 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 18:30:39 -0400 14, 27 -- Received: (qmail 29706 invoked by uid 89); 18 Apr 2008 22:30:36 -0000 14, 27 -- Received: from pitts-69-72-117-236.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.236) 14, 27 -- by mail.winbeam.com with SMTP; 18 Apr 2008 22:30:36 -0000 14, 27 -- Message-Id: {3.0.6.32.20080418193036.007ef100-at-pop3.norton.antivirus} 14, 27 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 14, 27 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 14, 27 -- Date: Fri, 18 Apr 2008 19:30:36 -0400 14, 27 -- To: microscopy-at-microscopy.com 14, 27 -- From: Beaurega {beaurega-at-westol.com} 14, 27 -- Subject: Re: [Microscopy] viaWWW: sputter coating 14, 27 -- Mime-Version: 1.0 14, 27 -- Content-Type: text/plain; charset="us-ascii" 14, 27 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 14, 27 -- X-MailScanner-ID: m3IMUchG026293 14, 27 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 14, 27 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 14, 27 -- SpamAssassin (not cached, score=-1.851, required 4, 14, 27 -- autolearn=not spam, AWL 0.15, BAYES_00 -2.00) 14, 27 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
There is a new bench-top high vacuum platform for research & development from Oxford Vacuum Science, it uses thermal evaporation, providing a real, low-cost alternative to electron beam technology:
www.oxford-vacuum.com
Best regards. Dietrich
} At 08:05 AM 4/18/08 -0500, you wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } replying } } please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: emer.ryan-at-dit.ie } } Name: Emer } } } } Organization: CREST DIT } } } } Title-Subject: [Filtered] sputter coating } } } } Question: Dear all, a question regarding applying conductive coatings. } We're in the process of procuring 2 new FESEMs and I need to update our } sample prep equipment. } } We have an Edwards Auto 306 and a vintage (workhorse)Poloran E5000. I am } aware that we need to update to a new sputter coater with e.g. Pt/Pd } targets; however it has been suggested to me that we use the Edwards } Evaporator for all our coatings needs. } } I don't think that this will suffice - but apart from the evaporator being } slower, can anyone help me build a list of reasons why we need a new } sputter coater? Input greatly appreciated. } } } } } } Login Host: 147.252.66.47 } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Fri Apr 18 08:04:39 2008 } } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m3ID4bm9019216 } } 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 } 08:04:39 -0500 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: {p06240804c42e4cb00c24-at-[206.69.208.22]} } } 7, 11 -- Date: Fri, 18 Apr 2008 08:04:37 -0500 } } 7, 11 -- To: microscopy-at-microscopy.com } } 7, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver) } } 7, 11 -- Subject: viaWWW: sputter coating } } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } } ==============================End of - Headers============================== } } } } } } } } ==============================Original Headers============================== } 14, 27 -- From beaurega-at-westol.com Fri Apr 18 17:33:24 2008 } 14, 27 -- Received: from smtp-gateway-6.winbeam.com } (smtp-gateway-6.winbeam.com [64.84.96.16] (may be forged)) } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m3IMXOk0000570 } 14, 27 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 17:33:24 } -0500 } 14, 27 -- X-Winbeam-MailScanner-Watermark: } 1209162654.89053-at-Ren78967hVQyU9L8WAWFCg } 14, 27 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) } 14, 27 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP id } m3IMUchG026293 } 14, 27 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 18:30:39 } -0400 } 14, 27 -- Received: (qmail 29706 invoked by uid 89); 18 Apr 2008 22:30:36 } -0000 } 14, 27 -- Received: from pitts-69-72-117-236.dynamic-dialup.coretel.net } (HELO millenium) (69.72.117.236) } 14, 27 -- by mail.winbeam.com with SMTP; 18 Apr 2008 22:30:36 -0000 } 14, 27 -- Message-Id: {3.0.6.32.20080418193036.007ef100-at-pop3.norton.antivirus} } 14, 27 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus } 14, 27 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) } 14, 27 -- Date: Fri, 18 Apr 2008 19:30:36 -0400 } 14, 27 -- To: microscopy-at-microscopy.com } 14, 27 -- From: Beaurega {beaurega-at-westol.com} } 14, 27 -- Subject: Re: [Microscopy] viaWWW: sputter coating } 14, 27 -- Mime-Version: 1.0 } 14, 27 -- Content-Type: text/plain; charset="us-ascii" } 14, 27 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact } Technical Support for more information } 14, 27 -- X-MailScanner-ID: m3IMUchG026293 } 14, 27 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of } MailScanner) } 14, 27 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), } 14, 27 -- SpamAssassin (not cached, score=-1.851, required 4, } 14, 27 -- autolearn=not spam, AWL 0.15, BAYES_00 -2.00) } 14, 27 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 28 -- From didi-at-specs.com Fri Apr 18 18:06:34 2008 8, 28 -- Received: from mail11b.verio-web.com (mail11b.verio-web.com [204.202.242.87]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m3IN6YDa014131 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 18:06:34 -0500 8, 28 -- Received: from mx39.stngva01.us.mxservers.net (204.202.242.107) 8, 28 -- by mail11b.verio-web.com (RS ver 1.0.95vs) with SMTP id 3-0730949993 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 19:06:33 -0400 (EDT) 8, 28 -- Received: from unknown [161.58.148.83] (EHLO mmm1125.verio-web.com) 8, 28 -- by va1-mx39.stngva01.us.mxservers.net (mxl_mta-3.1.0-03) 8, 28 -- with ESMTP id 97929084.2306866080.14860.00-002.va1-mx39.stngva01.us.mxservers.net (envelope-from {didi-at-specs.com} ); 8, 28 -- Fri, 18 Apr 2008 19:06:33 -0400 (EDT) 8, 28 -- Received: (qmail 24753 invoked from network); 18 Apr 2008 23:06:32 -0000 8, 28 -- Received: from unknown (HELO elaine.specs.com) (72.77.197.187) 8, 28 -- by with SMTP; 18 Apr 2008 23:06:32 -0000 8, 28 -- Message-Id: {5.2.1.1.0.20080418190218.023b7df0-at-specs.com} 8, 28 -- X-Sender: didi-at-specs.com (Unverified) 8, 28 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 8, 28 -- Date: Fri, 18 Apr 2008 19:07:43 -0400 8, 28 -- To: Microscopy-at-microscopy.com 8, 28 -- From: Dietrich von Diemar {didi-at-specs.com} 8, 28 -- Subject: Re: viaWWW: sputter coating 8, 28 -- In-Reply-To: {200804182240.m3IMeSf1011321-at-ns.microscopy.com} 8, 28 -- Mime-Version: 1.0 8, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 28 -- X-Spam: [F=0.0100000000; S=0.010(2008040101); MH=0.500(2008040918)] 8, 28 -- X-MAIL-FROM: {didi-at-specs.com} 8, 28 -- X-SOURCE-IP: [161.58.148.83] 8, 28 -- X-SF-Loop: 1 ==============================End of - Headers==============================
I have an ISI WB-6 SEM in limbo. The final lens is shorted and we have moved on to a replacement SEM. I need to do something with the ISI but before dumping it, I thought some of you may want a shot at it.
It is 22 years old and shows some wear and tear. Most systems are OK, but it doesn't work unless the lens gets fixed, maybe it could be a parts machine for someone. If you are interested, let me know and we can figure out the best thing to do with it.
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to make a donation. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 6, 20 -- From jmkrupp-at-ucsc.edu Fri Apr 18 18:36:49 2008 6, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3INan2Z027448 6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 18:36:49 -0500 6, 20 -- Received: from [128.114.125.117] (HELO ucsc.edu) 6, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 6, 20 -- with ESMTPS id 32971409 for microscopy-at-microscopy.com; Fri, 18 Apr 2008 16:36:49 -0700 6, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 6, 20 -- with PIPE id 83902363; Fri, 18 Apr 2008 16:36:49 -0700 6, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 6, 20 -- Received: from [128.114.25.109] (account jmkrupp-at-ucsc.edu HELO [128.114.25.109]) 6, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 6, 20 -- with ESMTPA id 83902354 for microscopy-at-microscopy.com; Fri, 18 Apr 2008 16:36:45 -0700 6, 20 -- Mime-Version: 1.0 6, 20 -- Message-Id: {p0623090ec42edffdab58-at-[128.114.25.109]} 6, 20 -- Date: Fri, 18 Apr 2008 16:36:43 -0700 6, 20 -- To: microscopy-at-microscopy.com 6, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 6, 20 -- Subject: ISI WB-6 going, going ........ 6, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Your description of using the DV-502A for carbon evaporation and using a basket makes me wonder if you are using thermal evaporation and not sputtering. If attempting thermal evaporation, platinum metal alloys with tungsten basket material and does not evaporate at the tungsten BP; going hotter will cause the tungsten filament/basket to evaporate and burn out. That is one reason other methods were used for Pt evaporation. In the older units with thermal evaporation, including carbon, Pt is often evaporated by wrapping a bit of fine Pt wire around the turned down peg of one of the carbon rods, or else using Pt:C sintered pellets in the drilled out end of one of the rods - but I think that this last method is not widely done now - I think most mfgs have dropped the sintered pellets from their catalogs.
I'm looking at a DV-502A in my browser: http://www.msg.ucsf.edu/em/EMNEW2/equipment.html
and this one is equipped for E-beam evaporation: (http://www.prism.princeton.edu/PRISM_cleanroom/equip/denton/denton.htm)
Can you please clarify what equipment/method you are trying?
But.... You can get a Pt target for most sputter coaters and by simply changing the target, you can change the material. With a Polaron E5100 (an old unit with DC sputtering) we can change targets in a couple of minutes. Check to see if there is one available for your unit. I have a name you can contact who can refurb the target surface or supply complete targets for older units; let me know if that is useful.
Dale
Dale Callaham Umass -at- Amherst
kristi.majni-at-basf.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both kristi.majni-at-basf.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: kristi.majni-at-basf.com } Name: Kristi Majni } } Organization: BASF } } Title-Subject: [Filtered] Problems coating with Denton Vacuum Coater } } Question: Hello, } } I am a new user of SEM and am having problems getting a coating of platinum with my Denton Vacuum coater (DV-502A). I currently use a Technics Hummer V to coat with Au/Pd, but since it is pretty old I would like to use the Denton with Pt as backup, plus as I understand it Pt is } better to use for imaging. I have no problems coating with carbon using the Denton, but whenever I try to coat with Pt, I have trouble. The basket that the current runs through always breaks and instead of getting a nice } sputter, I just get a spark. I've tried turning up the current very very slowly and pausing before going each notch and that doesn't seem to work. Can anyone give me any good pointers? Thanks! } } Login Host: 144.29.1.16 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Fri Apr 18 16:50:20 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3ILoIdg019284 } 7, 11 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 16:50:18 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240800c42ec80af5d2-at-[206.69.208.22]} } 7, 11 -- Date: Fri, 18 Apr 2008 16:50:16 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: kristi.majni-at-basf.com (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Problems coating with Denton Vacuum Coater } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 20 -- From dac-at-research.umass.edu Sun Apr 20 09:15:18 2008 9, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3KEFIEt023860 9, 20 -- for {microscopy-at-microscopy.com} ; Sun, 20 Apr 2008 09:15:18 -0500 9, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 9, 20 -- (authenticated bits=0) 9, 20 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m3KEFElf028066 9, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 9, 20 -- Sun, 20 Apr 2008 10:15:14 -0400 9, 20 -- Message-ID: {480B5E13.9080809-at-research.umass.edu} 9, 20 -- Date: Sun, 20 Apr 2008 10:15:31 -0500 9, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 9, 20 -- MIME-Version: 1.0 9, 20 -- To: kristi.majni-at-basf.com, Microscopy List {microscopy-at-microscopy.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: Problems coating with Denton Vacuum Coater 9, 20 -- References: {200804182155.m3ILt2fE027779-at-ns.microscopy.com} 9, 20 -- In-Reply-To: {200804182155.m3ILt2fE027779-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
International Workshop on Electron Crystallography of Membrane Proteins,
UC Davis, California, USA, September 7-13, 2008
We have the pleasure of announcing the opening of registration for a one-week International Workshop on Electron Crystallography of Membrane Proteins to be held at UC Davis, California (Sep 7-13, 2008).
The workshop has two major aims: 1. Training: To provide a unique forum to train skilled biochemists and electron microscopists (PhD, Post docs and beyond), in membrane protein 2D crystallization, electron crystallographic data collection and data processing. Topics will cover all aspects of electron crystallography, including: - Membrane protein solubilisation and crystallization - Sample preparation for electron microscopy - Cryo-EM data collection (imaging and electron diffraction) - Data processing with the MRC, IPLT and 2dx software packages - Data evaluation and model building 2. Advancing the technology: To bring together many of the leading groups in electron crystallography to forge innovative collaborations for new technology development.
Workshop format: The workshop will feature lectures in the morning, practicals in the afternoon, student poster session before dinner, and science talks in the evening. Computer practicals will use machines in a local computer room. However, students are also invited to bring their own laptop computer (Linux or OSX), where we can try to install the image processing software packages and run the processing on your own computers during the workshop.
Program: The detailed program is available at http://2dx.org/workshop/2008 (click on the “Program” link top left). This workshop will be focusing exclusively on topics related to electron crystallography. Invited speakers include Anchi Cheng, Andreas Engel, Andreas Schenk, Ansgar Philippsen, Ben Hankamer, Bob Glaeser, Bryant Gipson, Catherine Venien- Bryan, Daniel Levy, Dieter Typke, Gyobu Nobuhiko, Hans Hebert, Henning Stahlberg, Herve Remigy, Iban Ubarretxena-Belandia, Ken Downing, Michael Landsberg, Nigel Browning, Thomas Walz, Werner Kühlbrandt, Xiangyan Zeng, and Yifan Cheng.
Registration: The workshop is currently limited to 20 participants. The fee for academic participants is US$200, which covers registration, breakfast and lunch during the workshop. Lodging is not included in the registration fee, and can be reserved either with the UC Davis campus guest accommodation (http://www.confhsg.ucdavis.edu/UGR.htm, only 16 units available at $65/night on a first come first serve basis), or with a local hotel (http://daviswiki.org/Hotels, we can assist in finding a hotel). Registration can be completed at http://2dx.org/workshop/2008/registration before the deadline of 1 June 2008.
Further information: For further information please contact Henning Stahlberg (HStahlberg-at-ucdavis.edu ) Tom Walz (twalz-at-hms.harvard.edu), or Ben Hankamer (b.hankamer-at-imb.uq.edu.au ). For assistance with the local organization, please contact Bryant Gipson (BRGipson-at-ucdavis.edu).
Henning Stahlberg, Molecular & Cellular Biology, Briggs Hall 5, University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab), Fax: +1-530-752 3085 mailto:HStahlberg-at-ucdavis.edu, Skype:henningstahlberg http://stahlberglab.ucdavis.edu http://2dx.org _____________________________________________________________
==============================Original Headers============================== 20, 26 -- From HStahlberg-at-ucdavis.edu Sun Apr 20 10:06:38 2008 20, 26 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3KF6crB006177 20, 26 -- for {Microscopy-at-microscopy.com} ; Sun, 20 Apr 2008 10:06:38 -0500 20, 26 -- Received: from [192.168.1.125] ([75.44.203.224]) 20, 26 -- (authenticated bits=0) 20, 26 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id m3KF4KA8029440 20, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 20, 26 -- Sun, 20 Apr 2008 08:06:02 -0700 (PDT) 20, 26 -- Message-Id: {3B9E1901-2E43-4C79-8650-E744525EB95D-at-ucdavis.edu} 20, 26 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu} 20, 26 -- To: Microscopy-at-microscopy.com 20, 26 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 20, 26 -- Mime-Version: 1.0 (Apple Message framework v919.2) 20, 26 -- Subject: Workshop on Electron Crystallography of Membrane Proteins, Sept. 7-13, 2008, at UC Davis 20, 26 -- Date: Sun, 20 Apr 2008 08:04:20 -0700 20, 26 -- Cc: "Gipson Bryant R." {BRGipson-at-ucdavis.edu} , 20, 26 -- Hankamer Ben {b.hankamer-at-imb.uq.edu.au} , 20, 26 -- Walz Tom {twalz-at-hms.harvard.edu} , 20, 26 -- Stahlberg Henning {HStahlberg-at-ucdavis.edu} 20, 26 -- X-Mailer: Apple Mail (2.919.2) 20, 26 -- X-Virus-Scanned: ClamAV version 0.92, clamav-milter version 0.92 on av5 20, 26 -- X-Virus-Status: Clean 20, 26 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3KF6crB006177 ==============================End of - Headers==============================
A possibility, considering you mentioned a re-calibration... Be certain the low energy end of your spectrum is properly inhibited. This is usually a "threshold" adjustment. Uninhibited noise at the low end (typically below Be/B) will be counted in the DT value.
Another possibility, as mentioned is a bad FET pre-amp. Another is poor dewar vacuum / blown thin window.
One thing that "got" me was when I forgot to be certain my IR camera illumination was off (it defaults to ON when the viewing program is executed). The detector can see the IR and goes berserk!
Good Luck! Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Friday, April 18, 2008 6:01 AM To: White, Woody N.
Dear all!
Today I have the impression that our EDX detector coupled to our Tecnai G20 TEM microscope is just going crazy. The Dead Time (DT) is constantly on 100% when I want to analyse a particle (objective aperture out). I use classical copper grids and I took care not to be near a grid bar while reading. Here is what I did to extend the diagnostic:
1) When I position the beam on a grid bar, the CPS (counts per second) go up to 300 000 and DT 100%, as expected 2) When I target the center of a grid hole with the objective aperture out, I get a count of 30 and DT 100% 3) When I take the specimen holder out and insert the objective aperture, I have a CPS of 5 and a DT of 100% 4) When I take the specimen holder out and take the objective aperture out, I have a CPS of 0 and a DT of 0%, but sometimes it goes suddenly to 100% then come back to 0% (frequency, about every 2 sec) 5) I can retract the detector, in this case I get the same result of point 4)
I am a biologist, so I cannot understand much of what is happening, but for me it look like the processor is down. What I wonder is why the counts look correct while the DT is crazy. How can I have a CPS of 5 and a DT of 100%???
I never let the dewar warm up (but there is a protection in this case) and the only thing I did recently is calibrate the instrument (last week).
The instrument is from EDAX. I contacted them 2 weeks ago to ask for instructions on how to calibrate it, but still got no answer. So I don't expect much help from their side.
Stephane
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==============================Original Headers============================== 9, 19 -- From nizets2-at-yahoo.com Fri Apr 18 04:51:30 2008 9, 19 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m3I9pUMv031459 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 18 Apr 2008 04:51:30 -0500 9, 19 -- Received: (qmail 19226 invoked by uid 60001); 18 Apr 2008 09:51:29 -0000 9, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 19 -- s=s1024; d=yahoo.com; 9, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Conten t-Type:Message-ID; 9, 19 -- b=xkkS8bqFM2bkq963ObsNsEM3c5+Npoxfpx8k3CWhhlvHjTsYfLE9jiIPVK7sg5MqXCoD5Z Rmvz0HFoS7n3/iWpevqJewl31DU3YbkCXxFhkaiFTVDtqyanAQrZeWzlqrnvrnR59xEnrnCq cE8t1XTNbMgyqb9DkizHIvgASZCaU=; 9, 19 -- X-YMail-OSG: RW0gKhwVM1mJGIckFdjX6WPOB1tiB9Y6jZ6MUo28E3cXAejykTEYGtMIJ3YMjc2Qi9IFN9R8 5hRpQb_DzLUvC7IaPBEH3udlsWBsy6K16Vmm2rHlu8cz3NuLRds- 9, 19 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Fri, 18 Apr 2008 02:51:29 PDT 9, 19 -- X-Mailer: YahooMailRC/902.40 YahooMailWebService/0.7.185 9, 19 -- Date: Fri, 18 Apr 2008 02:51:29 -0700 (PDT) 9, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 19 -- Subject: EDX going crazy 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- MIME-Version: 1.0 9, 19 -- Content-Type: text/plain; charset=us-ascii 9, 19 -- Message-ID: {844741.19051.qm-at-web37402.mail.mud.yahoo.com} ==============================End of - Headers============================== ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 29 -- From nwwhite-at-babcock.com Mon Apr 21 07:00:25 2008 2, 29 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3LC0OUv021001 2, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 21 Apr 2008 07:00:24 -0500 2, 29 -- Received: from ([131.184.13.224]) 2, 29 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.8749880; 2, 29 -- Mon, 21 Apr 2008 08:00:09 -0400 2, 29 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 29 -- Mon, 21 Apr 2008 08:00:09 -0400 2, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 29 -- Content-class: urn:content-classes:message 2, 29 -- MIME-Version: 1.0 2, 29 -- Subject: RE: [Microscopy] EDX going crazy 2, 29 -- Date: Mon, 21 Apr 2008 08:00:08 -0400 2, 29 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87B75-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 29 -- In-Reply-To: {200804181000.m3IA0f19010680-at-ns.microscopy.com} 2, 29 -- X-MS-Has-Attach: 2, 29 -- X-MS-TNEF-Correlator: 2, 29 -- Thread-Topic: [Microscopy] EDX going crazy 2, 29 -- Thread-Index: AcihOyakOsfLb1xFRc+LxB/xp2A28ACat60g 2, 29 -- References: {200804181000.m3IA0f19010680-at-ns.microscopy.com} 2, 29 -- To: {nizets2-at-yahoo.com} , 2, 29 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 2, 29 -- X-OriginalArrivalTime: 21 Apr 2008 12:00:09.0381 (UTC) FILETIME=[3F1D4D50:01C8A3A7] 2, 29 -- From: "White, Woody N." {nwwhite-at-babcock.com} 2, 29 -- Content-Type: text/plain; 2, 29 -- charset="us-ascii" 2, 29 -- Content-Transfer-Encoding: 8bit 2, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3LC0OUv021001 ==============================End of - Headers==============================
Coincidentally, I have worked with both of these instruments.
When you say "basket", I assume you are referring to the ones made up of a single strand of tungsten wire and shaped like a tornado. If so, that may be your problem, since the platinum (wire, I assume) does not make good contact with the tungsten basket in order to heat up and melt before the basket does.
Instead, I recommend using a triple-stranded, tungsten wire that has been bent into a V shape. In practice, you wind the platinum very tightly at the tip of the V that is pointed downward. After getting to very high vacuum (at least 10^-4 Pa), slowly heat up the V-filament until it turns a cherry red. Leave it at that point for 30 sec or so in order to achieve any outgassing. Then, continue bringing up the current very slowly until you see the Pt start to melt. At this point, be cautious, since too large of an increase in heating will cause the partially melted Pt to splatter off of the filament, showering your specimen with large chunks of Pt.
Continue increasing the heat to the tungsten filament until the Pt completely melts. If you are lucky, it will form a droplet suspended from the tip of the V; however, most often, with triple stranded wires, it simply disappears into the strands of the wires (by capillary action, I surmise). At this point, you can turn up the heat to nearly white-hot and get the melted Pt to evaporate. It is my understanding that if you wait too long, however, the Pt may anneal with the W of the filament, making it very difficult to evaporate.
Here is one source of the triple stranded, V-shaped filament (Electron Microscopy Sciences):
All providers of EM supplies should stock this item. So, go with your preferred vendor.
If you do not want to buy the filaments, you can make one from single stranded tungsten wire by carefully bending it into a V, like the commercial versions. Single stranded filaments DO work but you have to be even more careful since the Pt flies off much more prematurely than with the triple stranded wires. However, when properly melted, you will see a droplet of melted Pt suspended from the tip of the V. If you carefully observe the droplet through dark photographic film (to protect your eyes), as you increase the heat, it will start to spin. This is the point where it is starting to evaporate, so you need to increase the heat only a little more to get it to totally evaporate.
As you are aware, the filaments get quite bright and you must protect your eyesight by viewing the process through exposed and developed photographic films that are quite dark. Alternately, you could use welders glasses to view the event.
You should practice this a couple times before putting in a critical specimen. Once you master it, you can get high resolution coatings rather easily.
Good luck and let us know how it turned out.
JB
Title-Subject: [Filtered] Problems coating with Denton Vacuum Coater
Question: Hello,
I am a new user of SEM and am having problems getting a coating of platinum with my Denton Vacuum coater (DV-502A). I currently use a Technics Hummer V to coat with Au/Pd, but since it is pretty old I would like to use the Denton with Pt as backup, plus as I understand it Pt is better to use for imaging. I have no problems coating with carbon using the Denton, but whenever I try to coat with Pt, I have trouble. The basket that the current runs through always breaks and instead of getting a nice sputter, I just get a spark. I've tried turning up the current very very slowly and pausing before going each notch and that doesn't seem to work. Can anyone give me any good pointers? Thanks!
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 19, 20 -- From oshel1pe-at-cmich.edu Mon Apr 21 07:04:19 2008 19, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 19, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3LC4JIZ025997 19, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Apr 2008 07:04:19 -0500 19, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 19, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m3LCHY9N032726 19, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Apr 2008 08:17:34 -0400 19, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 19, 20 -- Mon, 21 Apr 2008 08:04:38 -0400 19, 20 -- Mime-Version: 1.0 19, 20 -- Message-Id: {f06240800c43233318cea-at-[141.209.160.249]} 19, 20 -- Date: Mon, 21 Apr 2008 08:04:16 -0400 19, 20 -- To: Microscopy-at-microscopy.com 19, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 19, 20 -- Subject: Fwd: RE: Problem with Denton DV-502 19, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 19, 20 -- X-OriginalArrivalTime: 21 Apr 2008 12:04:38.0390 (UTC) FILETIME=[DF74D160:01C8A3A7] 19, 20 -- X-CanItPRO-Stream: default 19, 20 -- X-Spam-Score: -3.8 () L_EXCH_MF,L_USD 19, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pst3-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pst3-at-lehigh.edu Name: Samson Penn
Organization: Lehigh University
Title-Subject: [Filtered] [SEM] In need of JEOL stage
Question: I am in need of an SEM stage from a 6400, 6300 or an 840 JEOL JSM. Please contact me. Thank you
A postdoctoral position is available at Northwestern University to work on Precession Electron Diffraction, as well as to perform collaborative transmission electron microscopy in conjunction with a number of other faculty and research groups. A strong background in transmission electron diffraction and microscopy is required, and a good working knowledge of dynamical diffraction would be useful.
To apply, send a copy of your CV including the names of three referees to L-marks-at-northwestern.edu
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dsplan-at-solohill.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dsplan-at-solohill.com Name: David Splan
Organization: SoloHill Engineering, Inc.
Title-Subject: [Filtered] Particle analysis
Question: I was hoping someone could look over what I am doing to analyze particles with ur new microscope and give me some hints or suggestions...
I am trying to compare the number of fines (particles less than 100 microns) between two samples- one was sieved with tween and one was not. Also trying to detect small particles stuck to the larger ones, which are 170 microns (our main product). We are trying to eliminate ALL fines, especially those adhering to the larger beads.
I do not see big differences in the number of fines under the microscope when the samples are viewed "as is", so I have had to extract the fines from each sample, run them over a filter and analyze the filters (inverted onto a slide or a 60mm culture dish).
I am using a Nikon TI-E inverted scope with motorized stage and NIS-elements software. I perform a scan of a 3mm x 3mm area, taking photos of 90 fields within that area. Using NIS-elements, I am able to count and sort based on object features. For detection of small particles adhered to larger ones, I am sorting by the "Roughness" object feature.
If anyone has any ideas or suggestions of other techniques I would appreciate it.
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Email: dsplan-at-solohill.com Name: David Splan
Organization: SoloHill Engineering, Inc.
Title-Subject: [Filtered] Measuring background
Question: Just wondering how people measure (and subtract) the background (scratches and other imperfections in the glass) when measuring particles on a microscope. Thanks.
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Email: gxx-at-u.washington.edu Name: Xiaoxia
Organization: The Univresity fo Washington
Title-Subject: [Filtered] EMI cancellation system for TEM
Question: Hello, I have a question about EMI cancellation system. We plan to buy one for our brand new FEI Tecnai F20 TEM, since EMI inside the room higher than 80nT, which affects STEM image resolution. Seemed likt it is easier to buy and install a cancellation than do the room sheild. I did some search, and find Spicer Consulting providing magnetic field cancellation system. But I am wondering how effective the cancellation system works. If any one has used this system before, could you share your opinon with me?
I take a bright field image (same focal plane as your subject, but a region where there is no data), and a dark image (same exposure time as your data image, but do not open the shutter to the camera) and then use imageJ with the Normalize plugin http://rsb.info.nih.gov/ij/plugins/normalize.html
David
On Apr 21, 2008, at 5:39 PM, dsplan-at-solohill.com wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both dsplan-at-solohill.com as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: dsplan-at-solohill.com } Name: David Splan } } Organization: SoloHill Engineering, Inc. } } Title-Subject: [Filtered] Measuring background } } Question: Just wondering how people measure (and subtract) the } background (scratches and other imperfections in the glass) when } measuring particles on a microscope. } Thanks. } } David Splan } } Login Host: 64.50.254.118 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Mon Apr 21 19:33:54 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m3M0Xql5013811 } 7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 21 Apr 2008 19:33:53 } -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240806c432e2dec08a-at-[206.69.208.22]} } 7, 11 -- Date: Mon, 21 Apr 2008 19:33:51 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: dsplan-at-solohill.com (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Measuring background } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Mon Apr 21 20:13:58 2008 5, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3M1DvhI020403 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 21 Apr 2008 20:13:58 -0500 5, 22 -- Received: from gandalfs_amavis (amavis6.email.arizona.edu [10.0.0.209]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 5704D606057 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 21 Apr 2008 18:13:56 -0700 (MST) 5, 22 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id AF620606051 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 21 Apr 2008 18:13:51 -0700 (MST) 5, 22 -- Message-Id: {F6FDA381-660C-45DB-BB9C-47E2ED9A6BF5-at-arizona.edu} 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- In-Reply-To: {200804220039.m3M0dqFF027299-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Mime-Version: 1.0 (Apple Message framework v919.2) 5, 22 -- Subject: Re: [Microscopy] viaWWW: Measuring background 5, 22 -- Date: Mon, 21 Apr 2008 18:13:50 -0700 5, 22 -- References: {200804220039.m3M0dqFF027299-at-ns.microscopy.com} 5, 22 -- X-Mailer: Apple Mail (2.919.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I'm looking for a price estimate for breaking down and packing for shipping a Philips CM-12 STEM. Vendor contacts welcome. No shipping yet, just "how much to pack up?" But ... Anyone want to buy a CM-12? Under vacuum, works, side-mount digital camera, old below-column digital camera, no x-ray.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 2, 20 -- From oshel1pe-at-cmich.edu Tue Apr 22 11:30:56 2008 2, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3MGUupq014029 2, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Apr 2008 11:30:56 -0500 2, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 2, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m3MGi87E028836 2, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Apr 2008 12:44:10 -0400 2, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 2, 20 -- Tue, 22 Apr 2008 12:31:15 -0400 2, 20 -- Mime-Version: 1.0 2, 20 -- Message-Id: {f06240811c433c2addd11-at-[141.209.160.249]} 2, 20 -- Date: Tue, 22 Apr 2008 12:30:53 -0400 2, 20 -- To: Microscopy-at-microscopy.com 2, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 20 -- Subject: cost estimate to pack a STEM 2, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 20 -- X-OriginalArrivalTime: 22 Apr 2008 16:31:16.0305 (UTC) FILETIME=[495EAC10:01C8A496] 2, 20 -- X-CanItPRO-Stream: default 2, 20 -- X-Spam-Score: -4 () L_EXCH_MF 2, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Algae in chiller - follow up
Question: Dear colleagues, As suggested by one of you, I ran some tests to make sure we had algae in our system. To my surprise, the composition of the deposit I removed from the hose was mainly Fe, Zn and Cu... I suppose they come from the water pipes, and that our filters have not been changed as often as they should have been. Appart from changing the filters, cleaning the water deposit of the chiller, is there anything else I can do to remove this deposit from inside the microscopes?
Thank you all once again...
Isabel Nogueira MicroLab ICEMS/IST Lisboa - Portugal isabel.nogueira-at-ist.utl.pt
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Email: zhiping_luo-at-hotmail.com Name: Zhiping Luo
Organization: Texas A&M University
Title-Subject: [Filtered] RE: EDX going crazy
Question: Stephane,
High deadtime is a common problem related to the EDS maintenance. Several possibilities for this:
1. Thick ice layer buildup on the window. Consult the manufacture how to remove it. For Oxford INCA, simply run the detector conditioning program (refer to http://www.oxinst.com/wps/wcm/resources/file/ebd6db4f1d7f723/EDS-Hardware-Explained.pdf). This should be done in weeks/months.
2. Detector needs correct re-calibration from time to time (in months/years).
If still not working, unlikely your service can be skipped.
Zhiping Luo Microscopy and Imaging Center Biological Sciences Building West 119 Texas A&M University, College Station, TX 77843-2257 Phone: (979) 845-1129 (main) or 862-2883 (direct) FAX: (979) 847-8933 E-mail: luo-at-tamu.edu or luo-at-mic.tamu.edu http://www.tamu.edu/mic
} Dear all! } } Today I have the impression that our EDX detector coupled to our Tecnai } G20 TEM microscope is just going crazy. } The Dead Time (DT) is constantly on 100% when I want to analyse a } particle (objective aperture out). } I use classical copper grids and I took care not to be near a grid bar } while reading. } Here is what I did to extend the diagnostic: } } 1) When I position the beam on a grid bar, the CPS (counts per second) } go up to 300 000 and DT 100%, as expected } 2) When I target the center of a grid hole with the objective aperture } out, I get a count of 30 and DT 100% } 3) When I take the specimen holder out and insert the objective } aperture, I have a CPS of 5 and a DT of 100% } 4) When I take the specimen holder out and take the objective aperture } out, I have a CPS of 0 and a DT of 0%, but sometimes it goes suddenly to } 100% then come back to 0% (frequency, about every 2 sec) } 5) I can retract the detector, in this case I get the same result of } point 4) } } I am a biologist, so I cannot understand much of what is happening, but } for me it look like the processor is down. } What I wonder is why the counts look correct while the DT is crazy. } How can I have a CPS of 5 and a DT of 100%??? } } I never let the dewar warm up (but there is a protection in this case) } and the only thing I did recently is calibrate the instrument (last } week). } } The instrument is from EDAX. I contacted them 2 weeks ago to ask for } instructions on how to calibrate it, but still got no answer. So I don't } expect much help from their side. } } Stephane
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Email: tatineni-at-ncat.edu Name: Balaji Tatineni
Organization: NCAT State University
Title-Subject: [Filtered] Chemistry
Question: I would like to measure the particle density per unit volume in TEM images.
I am not sure I am the only one that sent you a suggestion. I sent you the procedure and suggestion to determine if you were dealing with either green algae or green copper carbonate residues. Either case is possible. So that is absolutely the first task. I am glad that someone found out what I did. Green does not mean that algae is the only cause of green deposits. See the Microscopy.com archives on Jan 21, 2008 for algae and carbonate reactions involving CO2.
As shown in the attachment article sent to Microscopy Today and you, the chemical reaction of CO2 will attack copper sources including brass. I suspect that your zinc is from alloys like brass and not galvanized pipes. Impellers on pumps can also be bronze and slowly eroded away over the years. So tin can also be found. The iron could be from a lot of sources but stainless steel fittings or tubing makes a lot of sense. Chlorides will pit corrode stainless steel. You can never eliminate all sources of copper, it's alloys, and iron in instruments or chiller loops.
Water filters can catch precipitates in suspension such as calcium and magnesium carbonates (city water contamination) and/or copper carbonate (erosion of copper and its alloys). A water filter will not stop the scaling by itself but is required at the inlet of every instrument, IMO. The scaling problem is Ksp based and that involves dissolved ion concentrations.
Removing the residual or plugging deposits that you can't see is tricky. Servicemen use Lime Away or CLR to remove the scale. Both these products contain a mild acid. They are citric acid and phosphoric acid based. FYI, some phosphates can be insoluble.
Dilute hydrochloric acid works to remove carbonates but it has some risk. You run a water circulation loop into an open bucket using a small circulating pump. Start out with a low concentration of HCl. All sorts of CO2 bubbles and black rubber particles can be seen in a white bucket exiting the return line. In the bottom of the bucket, put a couple of old dark copper US pennies or other equivalent copper coins. They must be oxidized. Once the initial bubbles are formed and any blockage is opened up a bit, you can then slowly add HCl while still circulating. Do not dump HCl additions onto the copper coins. Remove them temporarily while adding HCl. When you see the copper penny coins turn bright and the copper oxide is removed by the return line solution, then that is the highest HCl concentration you should use. You have started to attack the copper tubing oxides in your microscope and the pennies are your indicator for that attack. Immediately switch to pure DI water from another bucket and flush the system. Do not recirculate any water at this point. This flushing can take a while and requires frequent water dumping and refilling of the buckets. Monitor the output of the circulation from the microscope with silver nitrate to test for a white silver chloride precipitate. Once you think you have not detected any more chlorides from any residual HCl, flush a few more volumes of pure DI water and switch to freshly flushed chiller lines. Circulate fresh DI water for a few days. Test for chlorides. If you see chlorides, flush the system again. When you find no chlorides, add your algaecide, hopefully a low PPM level of a quaternary ammonium salt surfactant and not any chlorine based "bleach".
Try to seal the open reservoir airtight. It is the source of both CO2 reactions, whether algae or copper carbonate based. As CO2 is dissolved in the copper erosion case, the solubility product (Ksp) of copper carbonate will be exceeded before calcium carbonate. Once you exceed a Ksp, you will start to form scale. The ion concentrations will build up from CO2 mixing in the open reservoir, even in a HVAC heat exchanger based system. Monitor any transparent lines for a green color build up in the future. If you see the green build up, then you did not exchange the old water with new DI water often enough (3-6 months). You must replace the DI water periodically to prevent exceeding the Ksp of any carbonates that are sure to form.
It helps to have valves installed so that one can selectively flush the various branch lines to a diffusion pump or lenses. Deposits do not form uniformly. The output from DPs will scale more than the inlets. It would seem that hot water would keep the ions in solution. That is not what I/we found. So the green monitor tubing line should be after a DP, if possible. Check all the flow rates after cleaning out any lines. Check them agaisnt the flow specifications of the manufacturer.
You said that you were surprised at the composition of the green deposits. I was not. I spent two months killing alage that didn't exist by following my supervisor's algae idea. The more chloramine-T we added, the darker green the water turned. I took our water and added HCL and then ammonium hydroxide. The water turned green in acid and blue in NH4OH base. After a few blue and green color cycles back and forth, I looked at him and said, "I don't think we have a blue-green alga problem in our chiller. This is how dissolved copper ions perform chemically."
Like you., I got the message and eventually did some relevant inorganic testing. I would recommend having a serviceman flush the scale out with acid. If you have a good idea of how the lines run in the scope, then you can try descaling yourself.
JMO,
Paul Beauregard
At 11:32 PM 4/22/08 -0500, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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I am cleaning out a cabinet and would like to get rid of surplus parts. I have an old Bosch video camera (PAL) for a Zeiss 10 TEM that I'd love to get rid of. It's probably from the 1980s.
We also used to use the microscope for cryo, and I'd be happy to get rid of the goniometer/cryo control unit and the microdose focus unit. I am not completely sure I can disconnect the microdose focus unit, but the goniometer control is off. May need repair.
I am not yet ready to part out the rest of the TEM. It is not running, but I know I can make it run for at least a few hours on about a day's notice, so I'm keeping it as long as I have the room for it!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 19 -- From tina-at-pbrc.hawaii.edu Wed Apr 23 15:08:21 2008 7, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3NK8Jg2017413 7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Apr 2008 15:08:20 -0500 7, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m3NK8G0R009498 7, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Apr 2008 10:08:17 -1000 (HST) 7, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m3NK8GDI009495 7, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 23 Apr 2008 10:08:16 -1000 (HST) 7, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 7, 19 -- Date: Wed, 23 Apr 2008 10:08:15 -1000 (HST) 7, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 19 -- X-Sender: tina-at-halia 7, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 7, 19 -- Subject: Old parts from Zeiss 10/A TEM 7, 19 -- Message-ID: {Pine.GSO.4.21.0804231003270.9315-100000-at-halia} 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Among other things, we've got a couple of older AAs (2 Varian Spectra AA 400s; 1 flame, 1 graphite furnace) that are in marginal shape but still work. I know that Varian does not make parts for them anymore so they could be a valuable resource. We're working on acquiring some new instrumentation and are clearing house at the moment. I want to get rid of these but don't want to just throw them out. Is anyone aware of any surplus dealers or organizations who will take items like these off your hands to keep them available?
Cheers,
Rob Dean Geology Technician Kaufman Hall 148 Dickinson College P.O. Box 1773, Carlisle PA 17013 email: deanr-at-dickinson.edu phone: 717-245-1109 cell: 717-579-0644 fax: 717-245-1971
* Please consider your environmental responsibility before printing this e-mail or any other document.
==============================Original Headers============================== 8, 22 -- From deanr-at-dickinson.edu Wed Apr 23 15:44:43 2008 8, 22 -- Received: from exmail.dickinson.edu (exmail.DICKINSON.EDU [64.9.56.31]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3NKihLb031139 8, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Apr 2008 15:44:43 -0500 8, 22 -- Received: from DKNEXCCR.FAS.LCL ([172.16.152.32]) by dknexcas1.FAS.LCL 8, 22 -- ([172.16.152.21]) with mapi; Wed, 23 Apr 2008 16:44:40 -0400 8, 22 -- From: "Dean, Robert" {deanr-at-dickinson.edu} 8, 22 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 22 -- Date: Wed, 23 Apr 2008 16:44:43 -0400 8, 22 -- Subject: Misc. Old AAs/Lab equip 8, 22 -- Thread-Topic: Misc. Old AAs/Lab equip 8, 22 -- Thread-Index: AcilgtwJbkKk/Re2R/uukYqIfvwp1A== 8, 22 -- Message-ID: {DC785C1D16AD5C42A0987D89F09EF4BD01BA00DEF54F-at-DKNEXCCR.FAS.LCL} 8, 22 -- Accept-Language: en-US 8, 22 -- Content-Language: en-US 8, 22 -- X-MS-Has-Attach: 8, 22 -- X-MS-TNEF-Correlator: 8, 22 -- acceptlanguage: en-US 8, 22 -- Content-Type: text/plain; charset="iso-8859-1" 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Transfer-Encoding: 8bit 8, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3NKihLb031139 ==============================End of - Headers==============================
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Email: stroud-at-nrl.navy.mil Name: Rhonda Stroud
Organization: Naval Research Laboratory
Title-Subject: [Filtered] job opening: om/em characterization of metals
Question: The Materials Science and Technology Division of the Naval Research Laboratory has an immediate opening for a materials scientist to carry out OM/EM characterization of rapidly solidified metallic alloys. The position is initially offered at the contractor level, but conversion to civil service is expected. A Ph. D. in Materials Science, Physics or Mechanical Engineering is required.
I am looking to convert from tungsten filaments to LaB6 for a Philips CM-100 TEM. I would like opinions as to use experiences with filaments from different sources.
Is there a particular source that you have found that produces more reliable filaments with good life-times? What is the anticipated lifetime? This particular microscope tends to have a very good column vacuum.
Have you had any problems with student use in a multi-user facility? One reason that I have stuck with tungsten is that they are inexpensive and easily changed... Minimal expense if students over-saturate or saturate too quickly and shorten life-time.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 7, 21 -- From dsherman-at-purdue.edu Thu Apr 24 07:12:31 2008 7, 21 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3OCCVdl006413 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 07:12:31 -0500 7, 21 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 21 -- Thu, 24 Apr 2008 08:12:30 -0400 7, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 7, 21 -- Thu, 24 Apr 2008 12:12:30 +0000 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 7, 21 -- Date: Thu, 24 Apr 2008 08:12:30 -0400 7, 21 -- Subject: LaB6 filament use 7, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C435F16E.2BDDB%dsherman-at-purdue.edu} 7, 21 -- Thread-Topic: LaB6 filament use 7, 21 -- Thread-Index: AcimBHfMtjoHtxH3Ed2lmAARJN08Mg== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-OriginalArrivalTime: 24 Apr 2008 12:12:30.0955 (UTC) FILETIME=[785DC3B0:01C8A604] ==============================End of - Headers==============================
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Email: watsona-at-uthscsa.edu Name: Aaron M Watson
Organization: U Texas Health Science Center -at-SA
Title-Subject: [Filtered] sterilization by vacuum
Question: We are looking at cells on metals and different polymers and were wondering if anyone has use vacuum as a sterilization method as oppose to autoclaving. Under just UV we have had some bacterial contamination and some of the polymers do not do well with the temp of autoclaving. So I guess my question would be using vacuum would the surface become sterile enough to grow endolethial cells and monocytes?
Thank for your help in advance.
Aaron Watson Research Associate UTHSCSA San Antonio, TX
Vacuum alone will not sterilize your surface. It must be used in combination ethylene oxide (the real sterilizer). Are you using the correct UV wavelength (a real germicidal lamp)? Other ways to decontaminate surfaces would be: immersion in 70% ethanol for 5-10 minutes, gas sterilization using ethylene oxide (IF you know how to do this; otherwise, it's dangerous).
} Question: We are looking at cells on metals and different polymers } and were wondering if anyone has use vacuum as a sterilization method } as oppose to autoclaving. Under just UV we have had some bacterial } contamination and some of the polymers do not do well with the temp } of autoclaving. So I guess my question would be using vacuum would } the surface become sterile enough to grow endolethial cells and } monocytes?
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I am going to change the oil of our turbo (Pfeiffer TPU 270/330), but I don't have the manual of it. The pump has being used with our sputtering system for many year and the oil is really dirty. The question is, anyone know how I can drain the oil without removing the pump from the setup? It has two windows on both side of the pump and an access port on the middle of the window. This port can be removed, but I cannot drain the oil. At least not without tilting the pump.
It is a pretty old pump without the manual. If someone remember how the oil was drained it will be great. :) Thank you.
Carlos
-- Carlos Kazuo Inoki SUNY at Albany Deparment of Physics 1400 Washington Ave. Albany, NY 12222
==============================Original Headers============================== 5, 28 -- From kazuo-at-csc.albany.edu Thu Apr 24 19:59:09 2008 5, 28 -- Received: from smtp.albany.edu (mail1.its.albany.edu [169.226.1.105]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3P0x9uG021562 5, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 19:59:09 -0500 5, 28 -- Received: from mail-fe1.its.albany.edu (mail-fe1.its.albany.edu [169.226.1.167]) 5, 28 -- by smtp.albany.edu (8.13.8/8.13.8) with ESMTP id m3P0x6kp019975 5, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 20:59:09 -0400 (EDT) 5, 28 -- Received: (from webservd-at-localhost) 5, 28 -- by mail-fe1.its.albany.edu (8.13.8/8.13.8/Submit) id m3P0vlPG000782; 5, 28 -- Thu, 24 Apr 2008 20:57:47 -0400 (EDT) 5, 28 -- X-Authentication-Warning: mail-fe1.its.albany.edu: webservd set sender to kazuo-at-csc.albany.edu using -f 5, 28 -- Received: from 74.67.63.165 5, 28 -- (SquirrelMail authenticated user kazuo) 5, 28 -- by webmail.albany.edu with HTTP; 5, 28 -- Thu, 24 Apr 2008 20:57:47 -0400 (EDT) 5, 28 -- Message-ID: {46648.74.67.63.165.1209085067.squirrel-at-webmail.albany.edu} 5, 28 -- Date: Thu, 24 Apr 2008 20:57:47 -0400 (EDT) 5, 28 -- Subject: Pfeiffer turbopump oil change 5, 28 -- From: "Carlos Kazuo Inoki" {kazuo-at-csc.albany.edu} 5, 28 -- To: Microscopy-at-microscopy.com 5, 28 -- Reply-To: kazuo-at-csc.albany.edu 5, 28 -- User-Agent: SquirrelMail/1.4.11 5, 28 -- MIME-Version: 1.0 5, 28 -- Content-Type: text/plain;charset=iso-8859-1 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-Priority: 3 (Normal) 5, 28 -- Importance: Normal 5, 28 -- X-Scanned-By: MIMEDefang 2.64 on 169.226.1.44 ==============================End of - Headers==============================
} . . . We are looking at cells on metals and different polymers and } were wondering if anyone has use vacuum as a sterilization } method. . . So I guess my question would be using vacuum would the } surface become sterile enough to grow endolethial cells and monocytes?
If exposure to vacuum was sufficient, NASA wouldn't bother with their strict sterilization and cleaning procedures for space probes.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
I worked for Balzer/Pfeiffer for a number of years with this model TMP. If it's gone this long without a regular oil change, LEAVE IT BE. You are more likely to cause a failure by changing the oil than not. This is an obsolete pump with little chance of repair. If it fails you'll need to replace the pump & controller anyway. If it's not broken don't fix it.
Al Coritz Technical Engineer Electron Microscopy Sciences www.emsdiasum.com
-----Original Message----- X-from: kazuo-at-csc.albany.edu [mailto:kazuo-at-csc.albany.edu] Sent: Thursday, April 24, 2008 9:00 PM To: Sampleprep-at-earthlink.net
Hello,
I am going to change the oil of our turbo (Pfeiffer TPU 270/330), but I don't have the manual of it. The pump has being used with our sputtering system for many year and the oil is really dirty. The question is, anyone know how I can drain the oil without removing the pump from the setup? It has two windows on both side of the pump and an access port on the middle of the window. This port can be removed, but I cannot drain the oil. At least not without tilting the pump.
It is a pretty old pump without the manual. If someone remember how the oil was drained it will be great. :) Thank you.
Carlos
-- Carlos Kazuo Inoki SUNY at Albany Deparment of Physics 1400 Washington Ave. Albany, NY 12222
==============================Original Headers============================== 5, 28 -- From kazuo-at-csc.albany.edu Thu Apr 24 19:59:09 2008 5, 28 -- Received: from smtp.albany.edu (mail1.its.albany.edu [169.226.1.105]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3P0x9uG021562 5, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 19:59:09 -0500 5, 28 -- Received: from mail-fe1.its.albany.edu (mail-fe1.its.albany.edu [169.226.1.167]) 5, 28 -- by smtp.albany.edu (8.13.8/8.13.8) with ESMTP id m3P0x6kp019975 5, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 20:59:09 -0400 (EDT) 5, 28 -- Received: (from webservd-at-localhost) 5, 28 -- by mail-fe1.its.albany.edu (8.13.8/8.13.8/Submit) id m3P0vlPG000782; 5, 28 -- Thu, 24 Apr 2008 20:57:47 -0400 (EDT) 5, 28 -- X-Authentication-Warning: mail-fe1.its.albany.edu: webservd set sender to kazuo-at-csc.albany.edu using -f 5, 28 -- Received: from 74.67.63.165 5, 28 -- (SquirrelMail authenticated user kazuo) 5, 28 -- by webmail.albany.edu with HTTP; 5, 28 -- Thu, 24 Apr 2008 20:57:47 -0400 (EDT) 5, 28 -- Message-ID: {46648.74.67.63.165.1209085067.squirrel-at-webmail.albany.edu} 5, 28 -- Date: Thu, 24 Apr 2008 20:57:47 -0400 (EDT) 5, 28 -- Subject: Pfeiffer turbopump oil change 5, 28 -- From: "Carlos Kazuo Inoki" {kazuo-at-csc.albany.edu} 5, 28 -- To: Microscopy-at-microscopy.com 5, 28 -- Reply-To: kazuo-at-csc.albany.edu 5, 28 -- User-Agent: SquirrelMail/1.4.11 5, 28 -- MIME-Version: 1.0 5, 28 -- Content-Type: text/plain;charset=iso-8859-1 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-Priority: 3 (Normal) 5, 28 -- Importance: Normal 5, 28 -- X-Scanned-By: MIMEDefang 2.64 on 169.226.1.44 ==============================End of - Headers==============================
Good thing water bears are not pathogenic! Sterilization is relative...
From http://en.wikipedia.org/wiki/Tardigrade
Water bears are able to survive in extreme environments that would kill almost any other animal. They can survive temperatures close to absolute zero[4], temperatures as high as 151°C (303°F), 1,000 times more radiation than any other animal[5], nearly a decade without water, and can also survive in a vacuum like that found in space.
Dale Callaham Umass -at- Amherst
watsona-at-uthscsa.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both watsona-at-uthscsa.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: watsona-at-uthscsa.edu } Name: Aaron M Watson } } Organization: U Texas Health Science Center -at-SA } } Title-Subject: [Filtered] sterilization by vacuum } } Question: We are looking at cells on metals and different polymers } and were wondering if anyone has use vacuum as a sterilization method } as oppose to autoclaving. Under just UV we have had some bacterial } contamination and some of the polymers do not do well with the temp } of autoclaving. So I guess my question would be using vacuum would } the surface become sterile enough to grow endolethial cells and } monocytes? } } Thank for your help in advance. } } Aaron Watson } Research Associate } UTHSCSA } San Antonio, TX } } Login Host: 129.111.64.63 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Thu Apr 24 17:26:12 2008 } 8, 11 -- Received: from [130.202.15.123] (msdvpn8.msd.anl.gov [130.202.238.72]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3OMQ7mC023975 } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 17:26:09 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c436b966277b-at-[10.0.0.246]} } 8, 11 -- Date: Thu, 24 Apr 2008 17:26:04 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: watsona-at-uthscsa.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: sterilization by vacuum } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (sven.ernst-at-student.curtin.edu.au) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, April 25, 2008 at 02:13:56 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both sven.ernst-at-student.curtin.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: sven.ernst-at-student.curtin.edu.au Name: Sven Ernst
Organization: Curtin University of Technology
Education: Undergraduate College
Location: Perth, WA, Australia
Title: FFTs in focussing and removing objective astigmatism in TEM
Question: Hi,
I was just wondering, how is a TEM focussed using fast Fourier transforms?
Also, how do FFTs compare to Fresnel fringes for for focussing and removing objective astigmatism at high and low magnifications?
Excuse the delay, but there has been a problem accessing the listserve.
There is actually a whole new family of these table-top models for you to choose from. In addition to the two that you mentioned, JEOL just announced a joint venture with Nikon for the NeoScope (to be sold through your local Nikon dealer/rep), and, of course, there is FEI's Phenom. Both the Phenom and the NeoScope were on display about a month ago at Pittcon (New Orleans). The "family" is segmenting itself: The T1000 and Evex systems are upgradable to EDS. Phenom and Neoscope are intended to be extensions of light microscopy, with greater depth of field and higher mags. Each has its unique advantages. I've covered them briefly in an article which is coming out in American Lab News this month (www.iscpubs.com should have an electronic copy) and will speak more about them at the upcoming M&M meeting (EM section: Nanotechnology). My recommendation: the normal pathway. Visit the websites and download the literature then ask for a demo to see which feels most comfortable and bes t meets your needs.
Let me know what you decide!
Caveat: No commercial interest.
Best regards, Barbara Foster, President
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through Sept 2008. Call us today for details.
Thanks to everyone for the response. I did manage to suck the oil from the pump. Just a piece of curiosity. I did try to get the syringe from our chemistry store. I did discover that now days we need to have a permission to purchase one. Even without the needles. And this includes doing all the paperwork and getting a few signs from the management people. Was much easier to buy a pipette instead. :)
Regards,
Carlos
-- Carlos Kazuo Inoki SUNY at Albany Deparment of Physics 1400 Washington Ave. Albany, NY 12222
==============================Original Headers============================== 6, 28 -- From kazuo-at-csc.albany.edu Fri Apr 25 10:56:11 2008 6, 28 -- Received: from smtp.albany.edu (mail1.its.albany.edu [169.226.1.105]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3PFuBHi005847 6, 28 -- for {microscopy-at-microscopy.com} ; Fri, 25 Apr 2008 10:56:11 -0500 6, 28 -- Received: from mail-fe1.its.albany.edu (mail-fe1.its.albany.edu [169.226.1.167]) 6, 28 -- by smtp.albany.edu (8.13.8/8.13.8) with ESMTP id m3PFuA4w011093 6, 28 -- for {microscopy-at-microscopy.com} ; Fri, 25 Apr 2008 11:56:10 -0400 (EDT) 6, 28 -- Received: (from webservd-at-localhost) 6, 28 -- by mail-fe1.its.albany.edu (8.13.8/8.13.8/Submit) id m3PFtJA8001420; 6, 28 -- Fri, 25 Apr 2008 11:55:19 -0400 (EDT) 6, 28 -- X-Authentication-Warning: mail-fe1.its.albany.edu: webservd set sender to kazuo-at-csc.albany.edu using -f 6, 28 -- Received: from 169.226.175.214 6, 28 -- (SquirrelMail authenticated user kazuo) 6, 28 -- by webmail.albany.edu with HTTP; 6, 28 -- Fri, 25 Apr 2008 11:55:19 -0400 (EDT) 6, 28 -- Message-ID: {49276.169.226.175.214.1209138919.squirrel-at-webmail.albany.edu} 6, 28 -- Date: Fri, 25 Apr 2008 11:55:19 -0400 (EDT) 6, 28 -- Subject: Re: [Microscopy] Pfeiffer turbopump oil change 6, 28 -- From: "Carlos Kazuo Inoki" {kazuo-at-csc.albany.edu} 6, 28 -- To: microscopy-at-microscopy.com 6, 28 -- Reply-To: kazuo-at-csc.albany.edu 6, 28 -- User-Agent: SquirrelMail/1.4.11 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain;charset=iso-8859-1 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-Priority: 3 (Normal) 6, 28 -- Importance: Normal 6, 28 -- X-Scanned-By: MIMEDefang 2.64 on 169.226.1.44 ==============================End of - Headers==============================
FFT from a thin amorphous film contains information of the objective phase contrast transfer function (PCTF). By fitting the 2D intensity distribution in a single FFT image, one can measure the amount of mean defocus and the focus variation as a function of the azimuth angle, i.e. astigmatism. This is how focus and astigmatism are measured using the FFT image.
Compared with the Fresnel fringe method, the FFT method is far more accurate at high magnification and high resolution conditions, with a typical accuracy of 1-2nm in both focus and astigmatism measurement. At low magnifications, Fresnel method is preferred since it is easy to do and the accuracy requirement is not high.
Also the FFT image can be used to do coma-free alignment, a must for obtaining HREM images under the right focus condition that can be correctly interpreted using the structural model. At low magnifications, rotation alignment is adequate.
Hope this helps.
Good luck!
Ming Pan Gatan, Inc. www.gatan.com
-----Original Message----- X-from: sven.ernst-at-student.curtin.edu.au [mailto:sven.ernst-at-student.curtin.edu.au] Sent: Friday, April 25, 2008 7:10 AM To: mpan-at-gatan.com
This Question was submitted to Ask-A-Microscopist by (sven.ernst-at-student.curtin.edu.au) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, April 25, 2008 at 02:13:56 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both sven.ernst-at-student.curtin.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: sven.ernst-at-student.curtin.edu.au Name: Sven Ernst
Organization: Curtin University of Technology
Education: Undergraduate College
Location: Perth, WA, Australia
Title: FFTs in focussing and removing objective astigmatism in TEM
Question: Hi,
I was just wondering, how is a TEM focussed using fast Fourier transforms?
Also, how do FFTs compare to Fresnel fringes for for focussing and removing objective astigmatism at high and low magnifications?
Debbie, My experience with LaB6 is on SEMs, which operate at higher temperatures and emissions. I prefer the cathodes from Kimball Physics http://www.kimphys.com/index.htm Once the proper filament current is established, just leave it on. The SEMs typically run 40-60 µA and I just replace them at each semi-annual routine visit. I believe TEMs run at lower emission currents, therefore lower temperatures and longer life. As for multiple users, just tell them they CAN'T touch the filament temperature anymore. I'm sure a lot of tears will be shed over that.
The Kimball website also has a number of downloadable documents that are very helpful in understanding some of the particular peculiarities of LaB6 in general, and their design in particular.
No financial interest beyond keeping my own customers happy.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Thursday, April 24, 2008 8:16 AM To: kenconverse-at-qualityimages.biz
Hi all,
I am looking to convert from tungsten filaments to LaB6 for a Philips CM-100 TEM. I would like opinions as to use experiences with filaments from different sources.
Is there a particular source that you have found that produces more reliable filaments with good life-times? What is the anticipated lifetime? This particular microscope tends to have a very good column vacuum.
Have you had any problems with student use in a multi-user facility? One reason that I have stuck with tungsten is that they are inexpensive and easily changed... Minimal expense if students over-saturate or saturate too quickly and shorten life-time.
Debby
Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 7, 21 -- From dsherman-at-purdue.edu Thu Apr 24 07:12:31 2008 7, 21 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3OCCVdl006413 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 24 Apr 2008 07:12:31 -0500 7, 21 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 21 -- Thu, 24 Apr 2008 08:12:30 -0400 7, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 7, 21 -- Thu, 24 Apr 2008 12:12:30 +0000 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 7, 21 -- Date: Thu, 24 Apr 2008 08:12:30 -0400 7, 21 -- Subject: LaB6 filament use 7, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C435F16E.2BDDB%dsherman-at-purdue.edu} 7, 21 -- Thread-Topic: LaB6 filament use 7, 21 -- Thread-Index: AcimBHfMtjoHtxH3Ed2lmAARJN08Mg== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-OriginalArrivalTime: 24 Apr 2008 12:12:30.0955 (UTC) FILETIME=[785DC3B0:01C8A604] ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From kenconverse-at-qualityimages.biz Fri Apr 25 17:21:03 2008 21, 25 -- Received: from mta13.adelphia.net (mta13.mail.adelphia.net [68.168.78.44]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3PML2T4015333 21, 25 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 25 Apr 2008 17:21:02 -0500 21, 25 -- Received: from Ken ([72.227.100.25]) by mta13.adelphia.net 21, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 21, 25 -- id {20080425222022.LSVK21875.mta13.adelphia.net-at-Ken} ; 21, 25 -- Fri, 25 Apr 2008 18:20:22 -0400 21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 25 -- To: {dsherman-at-purdue.edu} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 21, 25 -- Subject: RE: [Microscopy] LaB6 filament use 21, 25 -- Date: Fri, 25 Apr 2008 18:20:49 -0400 21, 25 -- Message-ID: {000001c8a722$9dfd29c0$6401a8c0-at-Ken} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="iso-8859-1" 21, 25 -- X-Priority: 3 (Normal) 21, 25 -- X-MSMail-Priority: Normal 21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 21, 25 -- Importance: Normal 21, 25 -- Thread-Index: AcimBQKtyUIdXT+GTouHPfIPa3oJUQBGyGog 21, 25 -- In-Reply-To: {200804241216.m3OCGKHA010584-at-ns.microscopy.com} 21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3PML2T4015333 ==============================End of - Headers==============================
Sending for a colleague. Please respond to jinsong-wu-at-northwestern.edu, NOT to me!
Shuyou
=================
Postdoctoral Fellow in Nanotechnology at Northwestern University
We are now looking for a postdoctoral fellow, in the field of advanced analytical transmission electron microscopy (TEM). The postdoctoral fellow under the direction of Prof. Vinayak Dravid, Director of NUANCE Center will be doing advanced materials characterization, with a focus on applications in thermoelectric materials. With a recently installed FEI Helios NanoLab and the field emission JOEL-2100 TEM, the postdoc will explore the interface
of focused ion beam and analytical transmission electron microscopy. The expectation is that the postdoc will achieve high quality research results in the area of transmission electron microscopy and its research applications in materials science and engineering, with publication in highly regarded international journals.
The successful applicant will have earned a Ph.D. in materials science, physics, chemistry, geosciences, or a related field and have experience in the following: Experience required: 1. hands-on skills with TEM instrumentation and a solid experimental background in the applications of transmission electron microscopy 2. experience in scanning transmission electron microscopy (STEM) and EELS, high-resolution electron microscopy (HREM), and electron diffraction 3. TEM sample preparation 4. data analysis.
Send a resume or CV, a publication list, and names and contact information of three references to jinsong-wu-at-northwestern.edu. Northwestern University is an equal opportunity employer.
==============================Original Headers============================== 12, 19 -- From syli-at-northwestern.edu Fri Apr 25 18:15:22 2008 12, 19 -- Received: from merle.it.northwestern.edu (merle.it.northwestern.edu [129.105.16.57]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3PNFLMr029281 12, 19 -- for {microscopy-at-microscopy.com} ; Fri, 25 Apr 2008 18:15:21 -0500 12, 19 -- Received: from prep1 (navajo.ms.northwestern.edu [129.105.37.229]) 12, 19 -- by merle.it.northwestern.edu (Postfix) with ESMTP id AF604744F 12, 19 -- for {microscopy-at-microscopy.com} ; Fri, 25 Apr 2008 18:15:21 -0500 (CDT) 12, 19 -- From: "Shuyou Li" {syli-at-northwestern.edu} 12, 19 -- To: {microscopy-at-microscopy.com} 12, 19 -- Subject: Postdoctoral Fellow in Nanotechnology at Northwestern University 12, 19 -- Date: Fri, 25 Apr 2008 18:15:40 -0500 12, 19 -- Message-ID: {061d01c8a72a$473e7640$d5bb62c0$-at-edu} 12, 19 -- MIME-Version: 1.0 12, 19 -- Content-Type: text/plain; 12, 19 -- charset="us-ascii" 12, 19 -- Content-Transfer-Encoding: 7bit 12, 19 -- X-Mailer: Microsoft Office Outlook 12.0 12, 19 -- Thread-Index: AcinKkTFSWrENxx1TzWUzGFfn1H45g== 12, 19 -- Content-Language: en-us ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both steven-fischer-at-hotmail.co.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: steven-fischer-at-hotmail.co.uk Name: Steve
Title-Subject: [Filtered] SEM and LM Future of Microscopy
Question: Fellow microscopists
I would like to open a new thread to discuss the future of microscopy, applications and uses in problem solving etc.
I use both a research grade light microscope and a high resolution SEM (1.5kev to 30kev high vacuum) with elemental analysis capability. The samples I look at are mostly materials based: problem solving, research substances, contaminants etc as opposed to biological samples.
Are there any reasons to think that worldwide microscopy requirements are likely to fall off (economically, scientifically or technology.)
Are things headed towards a macroscopic view?
What other applications are there for microscopy?
Please be as detailed or succint as you wish.
The reason why I ask is that I am in the unfortunate position where I have to justify my job as a microscopist and would like to call upon the community to help with ideas to counter a redundancy.
FFT from any image contains frequency information (usually the phase information is not used). As such, an angular change in the Fourier domain (ex. 2-D intensity image) can represent astigmatism; and broadening in the Fourier domain represents spread of data. In general, the eye perceives a change in small frequencies as focus changes. Thus minimizing angular features minimizes astigmatism, and minimizing spread mathematically (stochastically) or visually by looking at a 2-D representation of the Fourier domain will improve focus.
John Russ in his The Image Processing Handbook book briefly touches on pieces of this [Disclaimer: I make no proceeds from this statement]. I have put together a presentation that includes these aspects for light microscopy, SEM, and TEM. Perhaps I'll try to clean it up and submit it for an article, but in the mean time I've sent it to you off-line (as no attachments are permitted on this listserve)
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
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-----Original Message----- X-from: mpan-at-gatan.com [mailto:mpan-at-gatan.com] Sent: Friday, April 25, 2008 5:35 PM To: ph2-at-sprynet.com
Dear Sven,
FFT from a thin amorphous film contains information of the objective phase contrast transfer function (PCTF). By fitting the 2D intensity distribution in a single FFT image, one can measure the amount of mean defocus and the focus variation as a function of the azimuth angle, i.e. astigmatism. This is how focus and astigmatism are measured using the FFT image.
Compared with the Fresnel fringe method, the FFT method is far more accurate at high magnification and high resolution conditions, with a typical accuracy of 1-2nm in both focus and astigmatism measurement. At low magnifications, Fresnel method is preferred since it is easy to do and the accuracy requirement is not high.
Also the FFT image can be used to do coma-free alignment, a must for obtaining HREM images under the right focus condition that can be correctly interpreted using the structural model. At low magnifications, rotation alignment is adequate.
Hope this helps.
Good luck!
Ming Pan Gatan, Inc. www.gatan.com
-----Original Message----- X-from: sven.ernst-at-student.curtin.edu.au [mailto:sven.ernst-at-student.curtin.edu.au] Sent: Friday, April 25, 2008 7:10 AM To: mpan-at-gatan.com
This Question was submitted to Ask-A-Microscopist by (sven.ernst-at-student.curtin.edu.au) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, April 25, 2008 at 02:13:56 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both sven.ernst-at-student.curtin.edu.au as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: sven.ernst-at-student.curtin.edu.au Name: Sven Ernst
Organization: Curtin University of Technology
Education: Undergraduate College
Location: Perth, WA, Australia
Title: FFTs in focussing and removing objective astigmatism in TEM
Question: Hi,
I was just wondering, how is a TEM focussed using fast Fourier transforms?
Also, how do FFTs compare to Fresnel fringes for for focussing and removing objective astigmatism at high and low magnifications?
I do not see a drop off in the workload on our microsopes. If anything, I see more people asking for more answers. Also, they want the answers yesterday.
just my two cents.....
Jim
PS: OoO away.............
} Email: steven-fischer-at-hotmail.co.uk } Name: Steve } } Title-Subject: [Filtered] SEM and LM Future of Microscopy } } Question: Fellow microscopists } } I would like to open a new thread to discuss the future of } microscopy, applications and uses in problem solving etc. } } I use both a research grade light microscope and a high resolution } SEM (1.5kev to 30kev high vacuum) with elemental analysis capability. } The samples I look at are mostly materials based: problem solving, } research substances, contaminants etc as opposed to biological } samples. } } Are there any reasons to think that worldwide microscopy requirements } are likely to fall off (economically, scientifically or technology.) } } Are things headed towards a macroscopic view? } } What other applications are there for microscopy? } } Please be as detailed or succint as you wish. } } The reason why I ask is that I am in the unfortunate position where I } have to justify my job as a microscopist and would like to call upon } the community to help with ideas to counter a redundancy. } } Many thanks. }
==============================Original Headers============================== 7, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Apr 26 13:08:34 2008 7, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3QI8YcU017145 7, 12 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 13:08:34 -0500 7, 12 -- Received: (from jquinn-at-localhost) 7, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m3QI8G916174 7, 12 -- for microscopy-at-microscopy.com; Sat, 26 Apr 2008 14:08:16 -0400 7, 12 -- Date: Sat, 26 Apr 2008 14:08:16 -0400 7, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 7, 12 -- Message-Id: {200804261808.m3QI8G916174-at-www.matscieng.sunysb.edu} 7, 12 -- To: microscopy-at-microscopy.com 7, 12 -- Subject: re: SEM and LM Future of Microscopy ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eyork-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eyork-at-ucsd.edu Name: Evelyn York
Organization: Scripps Institution of Oceanography
Title-Subject: [Filtered] E-SEM of Adipose Tissue
Question: We are trying to analyze adipose tissue using a environmental scanning electron microscope equipped with an energy dispersion spectrometer. First we tried processing these cells for HV SEM with various fixations, hydrations and using a critical point dryer. I am not an expert with these cells but after trying four different fixation protocols developed for high vacuum SEM I'd like to find a way to look at these with E-SEM. We are hoping one of you will know an appropriate protocol that you won't mind sharing with us off line.
I would like to invite you to submit cell biology images and videos to the Image & Video Library of the American Society for Cell Biology.
Have a look at our holdings: http://cellimages.ascb.org and follow the link under "Submit Resources" to set up an account and make your submissions. They will be reviewed by a member of our Editorial Board.
We're currently developing a website called CellBASE (Kerry Bloom is the Ed-in-Chief) with resources targeted at more defined topics - we're still building the content, but you can have a look at http://cellbase.ascb.org .
I'm looking forward to your submissions! Please feel free to email or call if you have any questions.
Cheers, Dave
David L. Ennist, PhD Director, Digital Resources The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 TEL: 301-347-9315 FAX: 301-347-9310 email: DEnnist-at-ascb.org
==============================Original Headers============================== 8, 17 -- From DENNIST-at-ascb.org Mon Apr 28 08:36:06 2008 8, 17 -- Received: from ascb-3.ascb.local (smtp.ascb.org [65.216.147.136]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m3SDa6fc024328 8, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 28 Apr 2008 08:36:06 -0500 8, 17 -- Received: from ASCB_DOMAIN-MTA by ascb.ascb.org 8, 17 -- with Novell_GroupWise; Mon, 28 Apr 2008 09:36:28 -0400 8, 17 -- Message-Id: {s8159a9c.040-at-ascb.ascb.org} 8, 17 -- X-Mailer: Novell GroupWise Internet Agent 6.5.7 8, 17 -- Date: Mon, 28 Apr 2008 09:35:51 -0400 8, 17 -- From: "David Ennist" {DENNIST-at-ascb.org} 8, 17 -- To: {Microscopy-at-Microscopy.Com} 8, 17 -- Subject: LM, SEM, TEM - Invitation to submit images to ASCB cell 8, 17 -- biology Image & Video Library 8, 17 -- Mime-Version: 1.0 8, 17 -- Content-Type: text/plain; charset=US-ASCII 8, 17 -- Content-Transfer-Encoding: 7bit 8, 17 -- Content-Disposition: inline ==============================End of - Headers==============================
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Email: hbrinkies-at-swin.edu.au Name: Hans Brinkies
Organization: Swinburne, University of Technology - Australia
Title-Subject: [Filtered] That's it
Question: After more than 45 years in the in the field of electron microscopy I like to use this site to say thank you to my Australian and overseas colleagues for their advise and help over the years. I will be retiring on May 1st and say finally 'goodbye' to my hobby called 'microscopy'. But I am sure that I will be missing the many 'happy hours' we had.
Kind regards Hans G Brinkies Swinburne, University of Technology Hawthorn - Melbourne - Australia
Our workload has increased enormously (over ten-fold!) over the past 7-8 years and shows absolutely no signs of slowing down. If anything the growth is still accelerating, to the point where just keeping up and having reasonable turn-around time is becoming difficult.
The growth in nano-particle research is fueling much of this, but general EM seems to be also on the increase in our lab. The slump of a decade ago seems to be long over.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: jquinn-at-www.matscieng.sunysb.edu [mailto:jquinn-at-www.matscieng.sunysb.edu] Sent: Saturday, April 26, 2008 1:10 PM To: Tindall, Randy D.
Steve and company
I do not see a drop off in the workload on our microsopes. If anything, I see more people asking for more answers. Also, they want the answers yesterday.
just my two cents.....
Jim
PS: OoO away.............
} Email: steven-fischer-at-hotmail.co.uk } Name: Steve } } Title-Subject: [Filtered] SEM and LM Future of Microscopy } } Question: Fellow microscopists } } I would like to open a new thread to discuss the future of microscopy, } applications and uses in problem solving etc. } } I use both a research grade light microscope and a high resolution SEM } (1.5kev to 30kev high vacuum) with elemental analysis capability. } The samples I look at are mostly materials based: problem solving, } research substances, contaminants etc as opposed to biological samples. } } Are there any reasons to think that worldwide microscopy requirements } are likely to fall off (economically, scientifically or technology.) } } Are things headed towards a macroscopic view? } } What other applications are there for microscopy? } } Please be as detailed or succint as you wish. } } The reason why I ask is that I am in the unfortunate position where I } have to justify my job as a microscopist and would like to call upon } the community to help with ideas to counter a redundancy. } } Many thanks. }
==============================Original Headers============================== 7, 12 -- From jquinn-at-www.matscieng.sunysb.edu Sat Apr 26 13:08:34 2008 7, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3QI8YcU017145 7, 12 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 13:08:34 -0500 7, 12 -- Received: (from jquinn-at-localhost) 7, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m3QI8G916174 7, 12 -- for microscopy-at-microscopy.com; Sat, 26 Apr 2008 14:08:16 -0400 7, 12 -- Date: Sat, 26 Apr 2008 14:08:16 -0400 7, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 7, 12 -- Message-Id: {200804261808.m3QI8G916174-at-www.matscieng.sunysb.edu} 7, 12 -- To: microscopy-at-microscopy.com 7, 12 -- Subject: re: SEM and LM Future of Microscopy ==============================End of - Headers==============================
==============================Original Headers============================== 19, 25 -- From TindallR-at-missouri.edu Mon Apr 28 09:46:36 2008 19, 25 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3SEkakk020583 19, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2008 09:46:36 -0500 19, 25 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 19, 25 -- Mon, 28 Apr 2008 09:46:34 -0500 19, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 25 -- Content-class: urn:content-classes:message 19, 25 -- MIME-Version: 1.0 19, 25 -- Content-Type: text/plain; 19, 25 -- charset="US-ASCII" 19, 25 -- Subject: RE: [Microscopy] re: SEM and LM Future of Microscopy 19, 25 -- Date: Mon, 28 Apr 2008 09:46:33 -0500 19, 25 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD75C5-at-UM-XMAIL08.um.umsystem.edu} 19, 25 -- In-Reply-To: {200804261810.m3QIAJdN018885-at-ns.microscopy.com} 19, 25 -- X-MS-Has-Attach: 19, 25 -- X-MS-TNEF-Correlator: 19, 25 -- Thread-Topic: [Microscopy] re: SEM and LM Future of Microscopy 19, 25 -- Thread-Index: AcinyM1VqOm+DsZgREelmEODkeU9cQBdLdaA 19, 25 -- References: {200804261810.m3QIAJdN018885-at-ns.microscopy.com} 19, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 19, 25 -- To: {microscopy-at-microscopy.com} 19, 25 -- X-OriginalArrivalTime: 28 Apr 2008 14:46:34.0229 (UTC) FILETIME=[A7707250:01C8A93E] 19, 25 -- Content-Transfer-Encoding: 8bit 19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3SEkakk020583 ==============================End of - Headers==============================
I'm back and I'm in the process of bringing back to life a microscope facility that has 1 TEM and 2 confocals. I'm looking for what y'all are currently charging users (all kinds campus users, industry and some what related to the university users).
If you would reply to me off the list that would be great. Just send me a listing of your current prices. If you could tell me what system you use to determine your rates that would be helpful too. I have to come up with a new price list and I want our prices to be in line with what others are charging.
Thanks! Remember to send me the info off-list.
Paula :-)
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I'd like to purchase additional welding googles for use while coating specimens in our new vacuum evaporator (via thermal evaporation using sharpened carbon rods). I have one old pair of welding googles, but I have no idea what shade they are (they don't appear to be labelled). Does anyone know the minimum shade value for this purpose? 5? 10? 15? Something in-between? I and other researchers in the lab enjoy seeing, so I'd like to choose the right shade.
Thanks, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
==============================Original Headers============================== 4, 17 -- From frah0010-at-umn.edu Mon Apr 28 23:10:41 2008 4, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3T4AeZD018334 4, 17 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2008 23:10:41 -0500 4, 17 -- Received: from [10.0.1.8] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206]) 4, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 4, 17 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2008 23:10:40 -0500 (CDT) 4, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN 4, 17 -- Message-Id: {9F18DB6B-FA54-4B33-8D5C-5E5FBD06CAEA-at-umn.edu} 4, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} 4, 17 -- To: microscopy-at-microscopy.com 4, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 17 -- Content-Transfer-Encoding: 7bit 4, 17 -- Mime-Version: 1.0 (Apple Message framework v919.2) 4, 17 -- Subject: Proper shade for vacuum evaporation 4, 17 -- Date: Mon, 28 Apr 2008 23:10:36 -0500 4, 17 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
Just thought I'd let you know, the problem was death of the power supply unit - our electronics workshop opened it up and it was all blackened in one corner.... Rather than paying the $2,200 for an exact replacement, we replaced it with a $150 unit from the local hardware shop - and the microtome works fine. We now have our instruments on UPS's so hopefully this won't happen again.
Original message: Our Leica UC6 will not turn on, and I wonder if a fuse has blown - but where would this be? I've inspected the power adapter, the control module (older touch-screen model) and microtome itself. Any ideas, anyone?
thanks, Rosemary White
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
You should get ANSI Publication Z87.1, "Practice for Occupational and Educational Eye and Face Protection". This may be an out of date reference, I'm taking out of my 7th edition welding handbook copyright 1984. But if you were to call them up and ask for that, they would know if it's been updated.
In the same book, there is a table 10.1 "Suggested viewing filter plates". Your specific process is not listed, but the process called "Air-carbon arc cutting" is probably close. For welding currents under 500 amps the minimum suggested shade is a 10. For above 500 amps the minimum suggested shade is 11. As an FYI, in all processes listed in this table, there are no shades listed above an 11 as the minimum shade required.
Good luck,
dj
On Mon, 28 Apr 2008, frah0010-at-umn.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'd like to purchase additional welding googles for use while coating } specimens in our new vacuum evaporator (via thermal evaporation using } sharpened carbon rods). I have one old pair of welding googles, but I } have no idea what shade they are (they don't appear to be labelled). } Does anyone know the minimum shade value for this purpose? 5? 10? } 15? Something in-between? I and other researchers in the lab enjoy } seeing, so I'd like to choose the right shade. } } Thanks, } Ellery } } -------------------- } Ellery E. Frahm } Research Fellow & Manager } Electron Microprobe Laboratory } University of Minnesota - Twin Cities } Department of Geology & Geophysics } Lab Website: http://probelab.geo.umn.edu } Personal Website: http://umn.edu/~frah0010 } } } ==============================Original Headers============================== } 4, 17 -- From frah0010-at-umn.edu Mon Apr 28 23:10:41 2008 } 4, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) } 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3T4AeZD018334 } 4, 17 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2008 23:10:41 -0500 } 4, 17 -- Received: from [10.0.1.8] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206]) } 4, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP } 4, 17 -- for {microscopy-at-microscopy.com} ; Mon, 28 Apr 2008 23:10:40 -0500 (CDT) } 4, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN } 4, 17 -- Message-Id: {9F18DB6B-FA54-4B33-8D5C-5E5FBD06CAEA-at-umn.edu} } 4, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} } 4, 17 -- To: microscopy-at-microscopy.com } 4, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 4, 17 -- Content-Transfer-Encoding: 7bit } 4, 17 -- Mime-Version: 1.0 (Apple Message framework v919.2) } 4, 17 -- Subject: Proper shade for vacuum evaporation } 4, 17 -- Date: Mon, 28 Apr 2008 23:10:36 -0500 } 4, 17 -- X-Mailer: Apple Mail (2.919.2) } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 21 -- From dljones-at-bestweb.net Tue Apr 29 05:12:44 2008 8, 21 -- Received: from mta4.srv.hcvlny.cv.net (mta4.srv.hcvlny.cv.net [167.206.4.199]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3TACiAQ028228 8, 21 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2008 05:12:44 -0500 8, 21 -- Received: from Pappee (ool-44c62883.dyn.optonline.net [68.198.40.131]) 8, 21 -- by mta4.srv.hcvlny.cv.net 8, 21 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 8, 21 -- with ESMTP id {0K0300D8A0D7PYM0-at-mta4.srv.hcvlny.cv.net} for 8, 21 -- microscopy-at-microscopy.com; Tue, 29 Apr 2008 06:12:44 -0400 (EDT) 8, 21 -- Date: Tue, 29 Apr 2008 06:12:52 -0400 (Eastern Daylight Time) 8, 21 -- From: "David L. Jones" {dljones-at-bestweb.net} 8, 21 -- Subject: Re: [Microscopy] Proper shade for vacuum evaporation 8, 21 -- In-reply-to: {200804290414.m3T4EmfU023816-at-ns.microscopy.com} 8, 21 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 8, 21 -- To: frah0010-at-umn.edu 8, 21 -- Cc: microscopy-at-microscopy.com 8, 21 -- Message-id: {Pine.WNT.4.64.0804290602270.5608-at-Pappee} 8, 21 -- MIME-version: 1.0 8, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 8, 21 -- Content-transfer-encoding: 7BIT 8, 21 -- References: {200804290414.m3T4EmfU023816-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi to all! I thought it may help others to share my experience. I tried dehydration and Epon embedding with only acetonitrile as agent. I used it instead of ethanol dehydration then PO (propylenoxide) then Epon in my classical protocol. Well...it didn't work. The sections contain holes or cracks which really look like bad polymerization. Specially artifacts (holes or cracks) are visible along the nuclear enveloppe and with lipidic organelles like the mucus-filled vacuoles of goblet cells (I tried with pieces of gut). Nothing is visible in LM on semi-thin sections, only under the TEM. Apart from the artifacts, the morphology is excellent and the contrast too. I can only guess that acetonitrile dehydration requires longer or more frequent steps than ethanol. My dehydration protocol for small pieces of organ in ethanol is 2x10' per step (50%-70%-95%-100%) and I did the same with acetonitrile. I could of course wash 3x or extend the incubation time but I wish not to experiment further with acetonitrile.
Enjoy your day Stephane
----- Original Message ---- X-from: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} To: nizets2-at-yahoo.com Sent: Thursday, April 17, 2008 2:30:40 PM
Hi to all!
I remember well that acetonitrile has been advised as a substitute for PO between ethanol dehydration and Epon embedding. I have always used ethanol and then PO until now but I am eager at using AN instead. Now I wonder if I could not use AN for dehydration too, so it could be used all along! For dehydration factors like penetration time, lipid extraction and hardening of the tissue are important to take into account but having no experience with AN I have no idea. Do some of you use AN for dehydration and Epon embedding? Are there differences between ethanol and AN dehydration (time, hardness,...) to take into account? Also, I have to split the whole procedure into 2 days, usually I stopped in ethanol 70%. Where should I stop until next day with AN?
Best regards, Stephane
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Good Morning: I operate a multi instrument pay for use facility. The income goes towards consumables, new equipment and service contracts. The question has been raised as to how I will charge users who require say 10 hours, embeddings, thin films or more. Currently I give a break (30%) to researchers who commit and pay up front for 50 filament hours but charge a flat fee for sample prep. Should I charge a flat fee for each immuno-label, whole mount and embedding or have a sliding scale once a certain number is reached? What about the cryo techniques such as thin films, freeze substitution and cryo sections a flat fee or sliding scale? What about the person who tells you at the start that he or she doesn't have enough money to pay the going rate? If you can get them great images they can publish and perhaps get grants to fund future work. Would you cut them off at a number of critical samples in hopes of future gain? I know we are all dealing with this and have seen it discussed on the listserver frequently. Does anyone have it figured out? If so please let us all know how you do things so we can too. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 6, 27 -- From bharris-at-uoguelph.ca Tue Apr 29 10:43:19 2008 6, 27 -- Received: from dargo.cs.uoguelph.ca (dargo.cs.uoguelph.ca [131.104.94.197]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3TFhIxT007030 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2008 10:43:18 -0500 6, 27 -- Received: from gimli.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 6, 27 -- by dargo.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m3TFhI4k012564 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2008 11:43:18 -0400 6, 27 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 6, 27 -- by gimli.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m3TFhDAO000387 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Apr 2008 11:43:18 -0400 6, 27 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca 6, 27 -- (Horde MIME library) with HTTP; Tue, 29 Apr 2008 11:43:13 -0400 6, 27 -- Message-ID: {20080429114313.t8krbxiskggs4wgg-at-webmail.uoguelph.ca} 6, 27 -- Date: Tue, 29 Apr 2008 11:43:13 -0400 6, 27 -- From: Robert J Harris {bharris-at-uoguelph.ca} 6, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 27 -- Subject: EM manager: Fixed vs Sliding rates 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset=ISO-8859-1; 6, 27 -- DelSp="Yes"; 6, 27 -- format="flowed" 6, 27 -- Content-Disposition: inline 6, 27 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 6, 27 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.197 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3TFhIxT007030 ==============================End of - Headers==============================
Dear Stephane, I have only ever substituted Acetonitrile in place of the PO, I've never tried to use it to dehydrate, only for the infiltration steps. It works great for that purpose. Good luck, Jo Dee
~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease
Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 29, 2008 8:15 AM To: jfish-at-gladstone.ucsf.edu
Hi to all! I thought it may help others to share my experience. I tried dehydration and Epon embedding with only acetonitrile as agent. I used it instead of ethanol dehydration then PO (propylenoxide) then Epon in my classical protocol. Well...it didn't work. The sections contain holes or cracks which really look like bad polymerization. Specially artifacts (holes or cracks) are visible along the nuclear enveloppe and with lipidic organelles like the mucus-filled vacuoles of goblet cells (I tried with pieces of gut). Nothing is visible in LM on semi-thin sections, only under the TEM. Apart from the artifacts, the morphology is excellent and the contrast too. I can only guess that acetonitrile dehydration requires longer or more frequent steps than ethanol. My dehydration protocol for small pieces of organ in ethanol is 2x10' per step (50%-70%-95%-100%) and I did the same with acetonitrile. I could of course wash 3x or extend the incubation time but I wish not to experiment further with acetonitrile.
Enjoy your day Stephane
----- Original Message ---- X-from: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} To: nizets2-at-yahoo.com Sent: Thursday, April 17, 2008 2:30:40 PM
Hi to all!
I remember well that acetonitrile has been advised as a substitute for PO between ethanol dehydration and Epon embedding. I have always used ethanol and then PO until now but I am eager at using AN instead. Now I wonder if I could not use AN for dehydration too, so it could be used all along! For dehydration factors like penetration time, lipid extraction and hardening of the tissue are important to take into account but having no experience with AN I have no idea. Do some of you use AN for dehydration and Epon embedding? Are there differences between ethanol and AN dehydration (time, hardness,...) to take into account? Also, I have to split the whole procedure into 2 days, usually I stopped in ethanol 70%. Where should I stop until next day with AN?
Best regards, Stephane
____________________________________________________________________________ ________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erin-at-aaisolutions.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: erin-at-aaisolutions.com Name: Erin Curry
Organization: Alliance Analytical
Title-Subject: [Filtered] Leica TCS SL Confocal available now!
Question: Hello all, Apologies for the off-topic post. We have a beautiful Leica TCS SL confocal that's just been brought up to spec by our Leica technician. We'd love to find it a new home and will certainly try to work within your budget if at all possible. Please contact Erin for photos and more details. Best regards, Erin Curry erin-at-aaisolutions.com (650) 324-2569
As other have pointed out, workload is not diminishing, but rather increasing.
But, what is changing, as I see, is the way microscopy is done. One think effectively more and more than one don't need a "microscopist"; than microscopes, as they work with a computers are just push button / click mouse devices. The origin is as much in the increasing workload, which need more time spent on the microscope, more than one man can give, as in the way some instruments are built, given the impression that "microsopist" is not a craft/trade ("métier" what's the adequate word in english ?). Everyone is able to do microscopy...
I'll give two illustrations :
First, all today EDS systems could be described, in an exagerate way, 5 fonctions : picture, acquire, ident, quant, report. The report gives results with two or three decimals, a total of 100.000 % etc... No need for a setup, nor for an analyste. Each fresh arrived student can do EDS,... and go back with poor results. But he/she doesn't kow how they are poor. There is no microanalyste anymore to explain him/her some basic rules. And he/she has so much to do trying to get results with other techniques like SEM, TEM, XRD, FTIR, Raman, SIMS, AES, VGT, AHHKB, RUIS, etc etc that he/she cannot learn everything.
Of coarse, I exagerate... but so fiew.
I see a second illustration in these new comming "desktop SEMs" about which there are much questions on the list. OK, I'll be the "vilain petit canard", the ugly duckling, which brings a false note in the music. But I would like to cartoon too these expensive toys : -take the best SEM you could build, -fix a price a bit higher than a good optical microscope and a bit lower than a low-end SEM -fix the profit you want to do, -simplify pitiless your SEM until it fits in the price -replace the maximum settings by auto functions -give the result to a designer, to create a fashoned look. You have now a click/mouse SEM with four functions : on, auto-focus, (auto)-mag, (auto)-capture. If you pay a bit more, the SEM will choose for your the right place of the sample and take a picture in full-auto mode at the best magnification, respecting the Imaging Gold Rules by the use of the new super build-in proprietary/patended WkEZY algorithm, etc... You don't need to think, buy and click !
An exageration, mmmmmhhh ? OK, I agree than there are specific domaines where such SEM may be useful, for exemple as field instruments, or for some routine observations. But there is much too noise about them, and I fear the main result they will produce is to enforce the false idee that one do not need a microscopist. Hey, a primary school child can now do SEM, why pay a microscopist !
Comming back to Steve's question, I must say that I understand it well. I have no fear for my job/position, but I see other places where things evoluate in the wrong way. The microscopist retire, is not replaced, everyone (or no-one, and the microscope is shut down) uses the microscope, and if one need it to do correct job, one have to clean, realigne, etc first. The results are missused equipement, poor or false results, which had could be right with the right settings, etc. One look after structurations in a polymere on pictures taken at 30 keV from a samples covered with 0.5 micron gold ! At the end, the methode itself can be discredited (EDS is not precise, is not accurate, etc. what have I soon heard). And it's not well accepted, if one have to say someone that he/she missused the methode. This point was evocated in a thread in the last weeks on the list.
Am I exagerating again ? Are other not confronted to such situations ?
Tell me I'm wrong, I've so the tendancy to be pessimistic ! ;-)
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
steven-fischer-at-hotmail.co.uk a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both steven-fischer-at-hotmail.co.uk as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: steven-fischer-at-hotmail.co.uk } Name: Steve } } Title-Subject: [Filtered] SEM and LM Future of Microscopy } } Question: Fellow microscopists } } I would like to open a new thread to discuss the future of } microscopy, applications and uses in problem solving etc. } } I use both a research grade light microscope and a high resolution } SEM (1.5kev to 30kev high vacuum) with elemental analysis capability. } The samples I look at are mostly materials based: problem solving, } research substances, contaminants etc as opposed to biological } samples. } } Are there any reasons to think that worldwide microscopy requirements } are likely to fall off (economically, scientifically or technology.) } } Are things headed towards a macroscopic view? } } What other applications are there for microscopy? } } Please be as detailed or succint as you wish. } } The reason why I ask is that I am in the unfortunate position where I } have to justify my job as a microscopist and would like to call upon } the community to help with ideas to counter a redundancy. } } Many thanks. } } } Login Host: 85.12.64.148 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 14, 27 -- From zaluzec-at-microscopy.com Sat Apr 26 08:08:29 2008 } 14, 27 -- Received: from sodium.colo.stayonline.net (neon.colo.stayonline.net [69.25.86.37]) } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3QD8T3c011105 } 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 08:08:29 -0500 } 14, 27 -- X-ASG-Debug-ID: 1209215299-737e005c0001-4CH8be } 14, 27 -- X-Barracuda-URL: http://192.168.5.11:8000/cgi-bin/mark.cgi } 14, 27 -- Received: from [172.16.0.236] (unknown [12.170.146.2]) } 14, 27 -- by sodium.colo.stayonline.net (Spam Firewall) with ESMTP id 69222264B68 } 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 09:08:20 -0400 (EDT) } 14, 27 -- Received: from [172.16.0.236] ([12.170.146.2]) by sodium.colo.stayonline.net with ESMTP id 6irR7OUfBFTki9Iq for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 09:08:20 -0400 (EDT) } 14, 27 -- Mime-Version: 1.0 } 14, 27 -- Message-Id: {p06240802c438d9babf80-at-[172.16.0.236]} } 14, 27 -- Date: Sat, 26 Apr 2008 08:08:25 -0500 } 14, 27 -- To: microscopy-at-microscopy.com } 14, 27 -- From: steven-fischer-at-hotmail.co.uk (by way of MicroscopyListserver) } 14, 27 -- X-ASG-Orig-Subj: viaWWW: SEM and LM Future of Microscopy } 14, 27 -- Subject: viaWWW: SEM and LM Future of Microscopy } 14, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 14, 27 -- X-Barracuda-Connect: UNKNOWN[12.170.146.2] } 14, 27 -- X-Barracuda-Start-Time: 1209215300 } 14, 27 -- X-Barracuda-Spam-Score: 0.10 } 14, 27 -- X-Barracuda-Spam-Status: No, SCORE=0.10 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests=RDNS_NONE } 14, 27 -- X-Barracuda-Spam-Report: Code version 3.1, rules version 3.1.48892 } 14, 27 -- Rule breakdown below } 14, 27 -- pts rule name description } 14, 27 -- ---- ---------------------- -------------------------------------------------- } 14, 27 -- 0.10 RDNS_NONE Delivered to trusted network by a host with no rDNS } ==============================End of - Headers============================== }
==============================Original Headers============================== 19, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Apr 30 06:44:27 2008 19, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UBiQoD030653 19, 31 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 06:44:26 -0500 19, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 19, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m3UBiNIM023731 19, 31 -- ; Wed, 30 Apr 2008 13:44:23 +0200 (CEST) 19, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 19, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 2F24710876B1; 19, 31 -- Wed, 30 Apr 2008 13:43:45 +0200 (CEST) 19, 31 -- Message-ID: {48185B70.90302-at-ipcms.u-strasbg.fr} 19, 31 -- Date: Wed, 30 Apr 2008 13:43:44 +0200 19, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 19, 31 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080306) 19, 31 -- MIME-Version: 1.0 19, 31 -- To: steven-fischer-at-hotmail.co.uk, Microscopy-at-microscopy.com 19, 31 -- Subject: Re: [Microscopy] viaWWW: Future of "the Microscopist" and some related 19, 31 -- comments about desktop SEMs 19, 31 -- References: {200804261317.m3QDHruL019550-at-ns.microscopy.com} 19, 31 -- In-Reply-To: {200804261317.m3QDHruL019550-at-ns.microscopy.com} 19, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 31 -- Content-Transfer-Encoding: 8bit 19, 31 -- X-IPCMS-MailScanner: Found to be clean 19, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 19, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 30 Apr 2008 13:44:23 +0200 (CEST) 19, 31 -- X-Virus-Scanned: ClamAV 0.93/6668/Tue Apr 8 13:57:49 2008 on mr2.u-strasbg.fr 19, 31 -- X-Virus-Status: Clean 19, 31 -- X-Spam-Status: No, score=1.5 required=5.0 tests=IP_LINK_PLUS,NORMAL_HTTP_TO_IP, 19, 31 -- WEIRD_PORT autolearn=disabled version=3.2.4 19, 31 -- X-Spam-Level: * 19, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr2.u-strasbg.fr ==============================End of - Headers==============================
We have the happy task of reorganizing our light microscopy labs and are seeking to replace our motley assortment of tables, benches and old computer desks with more ergonomically suitable ones for our light microscopes. To our surprise, Internet searches aren't turning up much and so we would love to hear from people who've found good solutions. We are hoping to find desks/tables that are adjustable in height without being knee-killers, have a cut-out in the table top, can accept casters for mobility, and ideally have a way to hold a computer underneath or on the side. If we were to get really greedy, we'd like an integrated power strip and a way to hold multiple flat screen monitors.
Any suggestions would be greatly appreciated.
Thanks, Pete
Peter Y. Eastman ? Microscopy Group ? Central Analytical Support ? peastman-at-rohmhaas.com -------------- Opinions expressed are mine and not those of Rohm and Haas Company --------------
==============================Original Headers============================== 5, 17 -- From PEastman-at-rohmhaas.com Wed Apr 30 07:03:27 2008 5, 17 -- Received: from EXTHO12.rohmhaas.com (extho12.rohmhaas.com [136.141.2.90]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UC3REw011463 5, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Apr 2008 07:03:27 -0500 5, 17 -- To: Microscopy-at-Microscopy.Com 5, 17 -- Subject: ergonomic microscope tables 5, 17 -- MIME-Version: 1.0 5, 17 -- X-Mailer: Lotus Notes 653HF134 February 24, 2005 5, 17 -- From: Peter Y Eastman {PEastman-at-rohmhaas.com} 5, 17 -- Message-ID: {OF859F9F85.AC2D1AD9-ON8525743B.0042106F-8525743B.00423AE9-at-rohmhaas.com} 5, 17 -- Date: Wed, 30 Apr 2008 08:03:24 -0400 5, 17 -- X-MIMETrack: Serialize by Router on EXTHO12/ROHEXT(Release 7.0.2FP2|May 14, 2007) at 04/30/2008 5, 17 -- 08:03:21 AM, 5, 17 -- Serialize complete at 04/30/2008 08:03:21 AM 5, 17 -- Content-Type: text/plain; charset="ISO-8859-1" 5, 17 -- Content-Transfer-Encoding: 8bit 5, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3UC3REw011463 ==============================End of - Headers==============================
I do only biological TEM and LM, but I find myself getting busier as time progresses. My role is morphing from strictly microscope work into technical support for LM, micro and macro imaging systems, and the software to run them. I also find myself in a consultant role for other departments buying microscopes that do not know the best configurations.
While I agree with another post that the basic operation of microscope/imaging system is becoming increasingly simpler, the basics of microscopy still elude most of the people that I interact with daily. One of the most common problems I fix for people is a misaligned condenser. It seems that no matter how many times I show them how to fix the problem, I will still get the call when it happens again.
Justifying your job is never a fun proposition, but then neither is letting a million dollars worth of equipment fall into disuse because nobody can get a good image from it.
Good luck, Steve
Steven Lee Chief Technologist Electron Microscopy Laboratory Baylor University Medical Center 3500 Gaston Ave Dallas, TX 75246 D 214.820.3302  214.820.4110 2 stevenle-at-baylorhealth.edu
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==============================Original Headers============================== 11, 30 -- From StevenLe-at-BaylorHealth.edu Wed Apr 30 07:31:21 2008 11, 30 -- Received: from bhdappagnt02.baylorhealth.edu (mailhost1.bhcs.com [65.248.93.160]) 11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UCVLKC025168 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 07:31:21 -0500 11, 30 -- Received: from BHDAEXIMS02.bhcs.pvt ([10.100.14.73]) 11, 30 -- by bhdappagnt02.baylorhealth.edu (8.14.1/8.14.1) with ESMTP id m3UCVKku011918 11, 30 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 07:31:20 -0500 11, 30 -- X-TM-IMSS-Message-ID: {46f9026b0004cafa-at-bhcs.pvt} 11, 30 -- Received: from BHDAEXHT01.bhcs.pvt ([10.5.12.193]) by bhcs.pvt ([10.100.14.73]) with ESMTP (TREND IMSS SMTP Service 7.0; TLS: TLSv1/SSLv3,128bits,RC4-MD5) id 46f9026b0004cafa ; Wed, 30 Apr 2008 07:31:21 -0600 11, 30 -- Received: from BHDAEXVM32.bhcs.pvt ([10.5.3.122]) by BHDAEXHT01.bhcs.pvt 11, 30 -- ([10.5.12.193]) with mapi; Wed, 30 Apr 2008 07:31:20 -0500 11, 30 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu} 11, 30 -- To: "steven-fischer-at-hotmail.co.uk" {steven-fischer-at-hotmail.co.uk} , 11, 30 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 30 -- Date: Wed, 30 Apr 2008 07:31:19 -0500 11, 30 -- Subject: Future of microscopy 11, 30 -- Thread-Topic: Future of microscopy 11, 30 -- Thread-Index: AciqvhdPbxNfds3XTxecjy286NAauQ== 11, 30 -- Message-ID: {492DD4D34D5DC5489E59D73B0506681C0B61067A02-at-BHDAEXVM32.bhcs.pvt} 11, 30 -- Accept-Language: en-US 11, 30 -- Content-Language: en-US 11, 30 -- X-MS-Has-Attach: 11, 30 -- X-MS-TNEF-Correlator: 11, 30 -- acceptlanguage: en-US 11, 30 -- Content-Type: text/plain; charset="utf-8" 11, 30 -- MIME-Version: 1.0 11, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7113:2.4.4,1.2.40,4.0.166 definitions=2008-04-30_05:2008-04-29,2008-04-30,2008-04-30 signatures=0 11, 30 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0804140000 definitions=main-0804300065 11, 30 -- Content-Transfer-Encoding: 8bit 11, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id m3UCVLKC025168 ==============================End of - Headers==============================
Thanks for taking one for the team! The excellent contrast you got must be because of little extraction...
I believe your original protocol was very good, that's what I use - ethanol and the PO. A rest in 70% ethanol - in the fridge, right?.. PO gives one a great piece of mind. It is a serious hazard in a teaching laboratory, but if it is just you and a few other people, all knowing what they are doing - I can't see why not use PO.
There we publications, you most likely know, where they used acetone instead of ethanol and got less extraction, as measured in collected fluids. I still prefer the ethanol, it smells much better...
Good day for you too, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Begin forwarded message:
} From: nizets2-at-yahoo.com } Date: April 29, 2008 11:04:54 AM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] Re: Acetonitrile as dehydration agent - Results } Reply-To: nizets2-at-yahoo.com } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi to all! } I thought it may help others to share my experience. } I tried dehydration and Epon embedding with only acetonitrile as } agent. I used it instead of ethanol dehydration then PO } (propylenoxide) then Epon in my classical protocol. } Well...it didn't work. The sections contain holes or cracks which } really look like bad polymerization. Specially artifacts (holes or } cracks) are visible along the nuclear enveloppe and with lipidic } organelles like the mucus-filled vacuoles of goblet cells (I tried } with pieces of gut). Nothing is visible in LM on semi-thin } sections, only under the TEM. } Apart from the artifacts, the morphology is excellent and the } contrast too. } I can only guess that acetonitrile dehydration requires longer or } more frequent steps than ethanol. } My dehydration protocol for small pieces of organ in ethanol is } 2x10' per step (50%-70%-95%-100%) and I did the same with } acetonitrile. } I could of course wash 3x or extend the incubation time but I wish } not to experiment further with acetonitrile. } } Enjoy your day } Stephane } } ----- Original Message ---- } X-from: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } To: nizets2-at-yahoo.com } Sent: Thursday, April 17, 2008 2:30:40 PM } Subject: [Microscopy] Acetonitrile as dehydration agent } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi to all! } } I remember well that acetonitrile has been advised as a substitute } for PO between ethanol dehydration and Epon embedding. } I have always used ethanol and then PO until now but I am eager at } using AN instead. Now I wonder if I could not use AN for } dehydration too, so it could be used all along! For dehydration } factors like penetration time, lipid extraction and hardening of } the tissue are important to take into account but having no } experience with AN I have no idea. } Do some of you use AN for dehydration and Epon embedding? Are there } differences between ethanol and AN dehydration (time, hardness,...) } to take into account? } Also, I have to split the whole procedure into 2 days, usually I } stopped in ethanol 70%. Where should I stop until next day with AN? } } Best regards, } Stephane } } } } ______________________________________________________________________ } ______________ } Be a better friend, newshound, and } know-it-all with Yahoo! Mobile. Try it now. http:// } mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ } } ==============================Original } Headers============================== } 5, 19 -- From nizets2-at-yahoo.com Thu Apr 17 07:26:19 2008 } 5, 19 -- Received: from web37401.mail.mud.yahoo.com } (web37401.mail.mud.yahoo.com [209.191.91.133]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
(But can you summarize this to a single bulleted item so we don't have to read and understand it?) :-)
Dale
jacques.faerber-at-ipcms.u-strasbg.fr wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Steve, and lister } } As other have pointed out, workload is not diminishing, but rather } increasing. } } But, what is changing, as I see, is the way microscopy is done. } One think effectively more and more than one don't need a } "microscopist"; than microscopes, as they work with a computers are just } push button / click mouse devices. The origin is as much in the } increasing workload, which need more time spent on the microscope, more } than one man can give, as in the way some instruments are built, given } the impression that "microsopist" is not a craft/trade ("métier" what's } the adequate word in english ?). Everyone is able to do microscopy... } } I'll give two illustrations : } } First, all today EDS systems could be described, in an exagerate } way, 5 fonctions : } picture, acquire, ident, quant, report. } The report gives results with two or three decimals, a total of 100.000 } % etc... No need for a setup, nor for an analyste. Each fresh arrived } student can do EDS,... and go back with poor results. But he/she } doesn't kow how they are poor. There is no microanalyste anymore to } explain him/her some basic rules. And he/she has so much to do trying to } get results with other techniques like SEM, TEM, XRD, FTIR, Raman, SIMS, } AES, VGT, AHHKB, RUIS, etc etc that he/she cannot learn everything. } } Of coarse, I exagerate... but so fiew. } } I see a second illustration in these new comming "desktop SEMs" about } which there are much questions on the list. } OK, I'll be the "vilain petit canard", the ugly duckling, which brings a } false note in the music. But I would like to cartoon too these expensive } toys : } -take the best SEM you could build, } -fix a price a bit higher than a good optical microscope and a bit } lower than a low-end SEM } -fix the profit you want to do, } -simplify pitiless your SEM until it fits in the price } -replace the maximum settings by auto functions } -give the result to a designer, to create a fashoned look. } You have now a click/mouse SEM with four functions : } on, auto-focus, (auto)-mag, (auto)-capture. } If you pay a bit more, the SEM will choose for your the right place of } the sample and take a picture in full-auto mode at the best } magnification, respecting the Imaging Gold Rules by the use of the new } super build-in proprietary/patended WkEZY algorithm, etc... } You don't need to think, buy and click ! } } An exageration, mmmmmhhh ? } OK, I agree than there are specific domaines where such SEM may be } useful, for exemple as field instruments, or for some routine } observations. But there is much too noise about them, and I fear the } main result they will produce is to enforce the false idee that one do } not need a microscopist. Hey, a primary school child can now do SEM, why } pay a microscopist ! } } } Comming back to Steve's question, I must say that I understand it well. } I have no fear for my job/position, but I see other places where things } evoluate in the wrong way. The microscopist retire, is not replaced, } everyone (or no-one, and the microscope is shut down) uses the } microscope, and if one need it to do correct job, one have to clean, } realigne, etc first. The results are missused equipement, poor or false } results, which had could be right with the right settings, etc. One look } after structurations in a polymere on pictures taken at 30 keV from a } samples covered with 0.5 micron gold ! } At the end, the methode itself can be discredited (EDS is not precise, } is not accurate, etc. what have I soon heard). And it's not well } accepted, if one have to say someone that he/she missused the methode. } This point was evocated in a thread in the last weeks on the list. } } Am I exagerating again ? Are other not confronted to such situations ? } } Tell me I'm wrong, I've so the tendancy to be pessimistic ! ;-) } } } Jacques } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } steven-fischer-at-hotmail.co.uk a écrit : } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both steven-fischer-at-hotmail.co.uk as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: steven-fischer-at-hotmail.co.uk } } Name: Steve } } } } Title-Subject: [Filtered] SEM and LM Future of Microscopy } } } } Question: Fellow microscopists } } } } I would like to open a new thread to discuss the future of } } microscopy, applications and uses in problem solving etc. } } } } I use both a research grade light microscope and a high resolution } } SEM (1.5kev to 30kev high vacuum) with elemental analysis capability. } } The samples I look at are mostly materials based: problem solving, } } research substances, contaminants etc as opposed to biological } } samples. } } } } Are there any reasons to think that worldwide microscopy requirements } } are likely to fall off (economically, scientifically or technology.) } } } } Are things headed towards a macroscopic view? } } } } What other applications are there for microscopy? } } } } Please be as detailed or succint as you wish. } } } } The reason why I ask is that I am in the unfortunate position where I } } have to justify my job as a microscopist and would like to call upon } } the community to help with ideas to counter a redundancy. } } } } Many thanks. } } } } } } Login Host: 85.12.64.148 } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 14, 27 -- From zaluzec-at-microscopy.com Sat Apr 26 08:08:29 2008 } } 14, 27 -- Received: from sodium.colo.stayonline.net (neon.colo.stayonline.net [69.25.86.37]) } } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3QD8T3c011105 } } 14, 27 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 4, 23 -- From dac-at-research.umass.edu Wed Apr 30 10:26:47 2008 4, 23 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UFQlVn025846 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 10:26:47 -0500 4, 23 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 4, 23 -- (authenticated bits=0) 4, 23 -- by race4.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m3UFQkxr002931 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 11:26:46 -0400 4, 23 -- Message-ID: {48189DFE.2070302-at-research.umass.edu} 4, 23 -- Date: Wed, 30 Apr 2008 11:27:42 -0500 4, 23 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 23 -- Reply-To: dac-at-research.umass.edu 4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 4, 23 -- MIME-Version: 1.0 4, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 23 -- Subject: Re: [Microscopy] Re: viaWWW: Future of "the Microscopist" and some 4, 23 -- related 4, 23 -- References: {200804301151.m3UBpXIj006758-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {200804301151.m3UBpXIj006758-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Jacques, What I've always loved about SEMs is that they are not microscopes but a signal generator and a signal processor. Tell me what you want to see and I can probably show it to you. Then we can sit down and have a long talk about whether or not it's real. This applies several times over to EDS. "But the computer said..." I really don't care what the computer said. If you don't understand the equipment, the physics of its signal generation and the many artifacts that can be generated, then you have no business putting out results that others, equally ignorant on the subject, are depending on. Sometimes lives depend on the results. Are we willing to pay for skilled people? Sadly, often the answer is no. Should we pay for skilled people? Absolutely yes.
The high level of automation has created a monster in more areas than just microanalysis. If it's easy to get pretty pictures and quantitative results to several decimal places, it must not require any skill, right? (and the quant results were off of a very rough fracture surface...must be accurate)
It seems everything from SEMs to the Wall Street Journal is being dumbed down. Sorry to be so pessimistic.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Wednesday, April 30, 2008 7:48 AM To: kenconverse-at-qualityimages.biz
Hi Steve, and lister
As other have pointed out, workload is not diminishing, but rather increasing.
But, what is changing, as I see, is the way microscopy is done. One think effectively more and more than one don't need a "microscopist"; than microscopes, as they work with a computers are just push button / click mouse devices. The origin is as much in the increasing workload, which need more time spent on the microscope, more than one man can give, as in the way some instruments are built, given the impression that "microsopist" is not a craft/trade ("métier" what's the adequate word in english ?). Everyone is able to do microscopy...
I'll give two illustrations :
First, all today EDS systems could be described, in an exagerate way, 5 fonctions : picture, acquire, ident, quant, report. The report gives results with two or three decimals, a total of 100.000 % etc... No need for a setup, nor for an analyste. Each fresh arrived student can do EDS,... and go back with poor results. But he/she doesn't kow how they are poor. There is no microanalyste anymore to explain him/her some basic rules. And he/she has so much to do trying to get results with other techniques like SEM, TEM, XRD, FTIR, Raman, SIMS, AES, VGT, AHHKB, RUIS, etc etc that he/she cannot learn everything.
Of coarse, I exagerate... but so fiew.
I see a second illustration in these new comming "desktop SEMs" about which there are much questions on the list. OK, I'll be the "vilain petit canard", the ugly duckling, which brings a false note in the music. But I would like to cartoon too these expensive toys : -take the best SEM you could build, -fix a price a bit higher than a good optical microscope and a bit lower than a low-end SEM -fix the profit you want to do, -simplify pitiless your SEM until it fits in the price -replace the maximum settings by auto functions -give the result to a designer, to create a fashoned look. You have now a click/mouse SEM with four functions : on, auto-focus, (auto)-mag, (auto)-capture. If you pay a bit more, the SEM will choose for your the right place of the sample and take a picture in full-auto mode at the best magnification, respecting the Imaging Gold Rules by the use of the new super build-in proprietary/patended WkEZY algorithm, etc... You don't need to think, buy and click !
An exageration, mmmmmhhh ? OK, I agree than there are specific domaines where such SEM may be useful, for exemple as field instruments, or for some routine observations. But there is much too noise about them, and I fear the main result they will produce is to enforce the false idee that one do not need a microscopist. Hey, a primary school child can now do SEM, why pay a microscopist !
Comming back to Steve's question, I must say that I understand it well. I have no fear for my job/position, but I see other places where things evoluate in the wrong way. The microscopist retire, is not replaced, everyone (or no-one, and the microscope is shut down) uses the microscope, and if one need it to do correct job, one have to clean, realigne, etc first. The results are missused equipement, poor or false results, which had could be right with the right settings, etc. One look after structurations in a polymere on pictures taken at 30 keV from a samples covered with 0.5 micron gold ! At the end, the methode itself can be discredited (EDS is not precise, is not accurate, etc. what have I soon heard). And it's not well accepted, if one have to say someone that he/she missused the methode. This point was evocated in a thread in the last weeks on the list.
Am I exagerating again ? Are other not confronted to such situations ?
Tell me I'm wrong, I've so the tendancy to be pessimistic ! ;-)
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
steven-fischer-at-hotmail.co.uk a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both steven-fischer-at-hotmail.co.uk as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: steven-fischer-at-hotmail.co.uk } Name: Steve } } Title-Subject: [Filtered] SEM and LM Future of Microscopy } } Question: Fellow microscopists } } I would like to open a new thread to discuss the future of } microscopy, applications and uses in problem solving etc. } } I use both a research grade light microscope and a high resolution } SEM (1.5kev to 30kev high vacuum) with elemental analysis capability. } The samples I look at are mostly materials based: problem solving, } research substances, contaminants etc as opposed to biological } samples. } } Are there any reasons to think that worldwide microscopy requirements } are likely to fall off (economically, scientifically or technology.) } } Are things headed towards a macroscopic view? } } What other applications are there for microscopy? } } Please be as detailed or succint as you wish. } } The reason why I ask is that I am in the unfortunate position where I } have to justify my job as a microscopist and would like to call upon } the community to help with ideas to counter a redundancy. } } Many thanks. } } } Login Host: 85.12.64.148 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 14, 27 -- From zaluzec-at-microscopy.com Sat Apr 26 08:08:29 2008 } 14, 27 -- Received: from sodium.colo.stayonline.net (neon.colo.stayonline.net [69.25.86.37]) } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3QD8T3c011105 } 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 08:08:29 -0500 } 14, 27 -- X-ASG-Debug-ID: 1209215299-737e005c0001-4CH8be } 14, 27 -- X-Barracuda-URL: http://192.168.5.11:8000/cgi-bin/mark.cgi } 14, 27 -- Received: from [172.16.0.236] (unknown [12.170.146.2]) } 14, 27 -- by sodium.colo.stayonline.net (Spam Firewall) with ESMTP id 69222264B68 } 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 09:08:20 -0400 (EDT) } 14, 27 -- Received: from [172.16.0.236] ([12.170.146.2]) by sodium.colo.stayonline.net with ESMTP id 6irR7OUfBFTki9Iq for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 09:08:20 -0400 (EDT) } 14, 27 -- Mime-Version: 1.0 } 14, 27 -- Message-Id: {p06240802c438d9babf80-at-[172.16.0.236]} } 14, 27 -- Date: Sat, 26 Apr 2008 08:08:25 -0500 } 14, 27 -- To: microscopy-at-microscopy.com } 14, 27 -- From: steven-fischer-at-hotmail.co.uk (by way of MicroscopyListserver) } 14, 27 -- X-ASG-Orig-Subj: viaWWW: SEM and LM Future of Microscopy } 14, 27 -- Subject: viaWWW: SEM and LM Future of Microscopy } 14, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 14, 27 -- X-Barracuda-Connect: UNKNOWN[12.170.146.2] } 14, 27 -- X-Barracuda-Start-Time: 1209215300 } 14, 27 -- X-Barracuda-Spam-Score: 0.10 } 14, 27 -- X-Barracuda-Spam-Status: No, SCORE=0.10 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests=RDNS_NONE } 14, 27 -- X-Barracuda-Spam-Report: Code version 3.1, rules version 3.1.48892 } 14, 27 -- Rule breakdown below } 14, 27 -- pts rule name description } 14, 27 -- ---- ---------------------- -------------------------------------------------- } 14, 27 -- 0.10 RDNS_NONE Delivered to trusted network by a host with no rDNS } ==============================End of - Headers============================== }
==============================Original Headers============================== 19, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Apr 30 06:44:27 2008 19, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UBiQoD030653 19, 31 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 06:44:26 -0500 19, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 19, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m3UBiNIM023731 19, 31 -- ; Wed, 30 Apr 2008 13:44:23 +0200 (CEST) 19, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 19, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 2F24710876B1; 19, 31 -- Wed, 30 Apr 2008 13:43:45 +0200 (CEST) 19, 31 -- Message-ID: {48185B70.90302-at-ipcms.u-strasbg.fr} 19, 31 -- Date: Wed, 30 Apr 2008 13:43:44 +0200 19, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 19, 31 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080306) 19, 31 -- MIME-Version: 1.0 19, 31 -- To: steven-fischer-at-hotmail.co.uk, Microscopy-at-microscopy.com 19, 31 -- Subject: Re: [Microscopy] viaWWW: Future of "the Microscopist" and some related 19, 31 -- comments about desktop SEMs 19, 31 -- References: {200804261317.m3QDHruL019550-at-ns.microscopy.com} 19, 31 -- In-Reply-To: {200804261317.m3QDHruL019550-at-ns.microscopy.com} 19, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 31 -- Content-Transfer-Encoding: 8bit 19, 31 -- X-IPCMS-MailScanner: Found to be clean 19, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 19, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 30 Apr 2008 13:44:23 +0200 (CEST) 19, 31 -- X-Virus-Scanned: ClamAV 0.93/6668/Tue Apr 8 13:57:49 2008 on mr2.u-strasbg.fr 19, 31 -- X-Virus-Status: Clean 19, 31 -- X-Spam-Status: No, score=1.5 required=5.0 tests=IP_LINK_PLUS,NORMAL_HTTP_TO_IP, 19, 31 -- WEIRD_PORT autolearn=disabled version=3.2.4 19, 31 -- X-Spam-Level: * 19, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr2.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 33, 26 -- From kenconverse-at-qualityimages.biz Wed Apr 30 10:38:26 2008 33, 26 -- Received: from mta15.adelphia.net (mta15.adelphia.net [68.168.78.77]) 33, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UFcPCd005799 33, 26 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 30 Apr 2008 10:38:25 -0500 33, 26 -- Received: from Ken ([72.227.100.25]) by mta15.adelphia.net 33, 26 -- (InterMail vM.6.01.05.04 201-2131-123-105-20051025) with ESMTP 33, 26 -- id {20080430153753.FUBM29625.mta15.adelphia.net-at-Ken} ; 33, 26 -- Wed, 30 Apr 2008 11:37:53 -0400 33, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 33, 26 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr} , 33, 26 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 33, 26 -- Subject: RE: [Microscopy] Re: viaWWW: Future of "the Microscopist" and some related 33, 26 -- Date: Wed, 30 Apr 2008 11:38:12 -0400 33, 26 -- Message-ID: {000501c8aad8$3390bac0$6401a8c0-at-Ken} 33, 26 -- MIME-Version: 1.0 33, 26 -- Content-Type: text/plain; 33, 26 -- charset="iso-8859-1" 33, 26 -- X-Priority: 3 (Normal) 33, 26 -- X-MSMail-Priority: Normal 33, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 33, 26 -- Importance: Normal 33, 26 -- Thread-Index: AciquAEl+0z3qpf+TImX6VhYUCzOAgAHW5cg 33, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 33, 26 -- In-Reply-To: {200804301147.m3UBlkY6002421-at-ns.microscopy.com} 33, 26 -- Content-Transfer-Encoding: 8bit 33, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3UFcPCd005799 ==============================End of - Headers==============================
I see an increase in microscope users and a decrease in microscopists. The first uses comparisons to do science the other induction. This will be the future of increased microscope (and associated instrument) use, that is systemize to maintain lower cost and bring tasks to the lowest common denominator in a business sense, leaving induction for the expert microscopists.
Ten years ago I submitted an op-ed style article to a journal entitled, "The Decline of the Light Microscope" (it was declined), from it I thought I'd pull a couple of pieces which I think are relevant to microscopy as a whole:
"Aristotle(1) wrote that,'In a practical science, so much depends on particular circumstances that only general rules can be given.' This is the epitome of microscopy. Secondly, in Lewis Carrol's Alice Through the Looking Glass(2), Humpty Dumpty responds to a question regarding a definition with,'When I use a word...it means just what I choose it to mean...'; so it is with science - the Humpty Dumpty debate. You have your truth and I have mine. Developing a truthful understanding of what science is and what are its limitations is crucial to defining science, and microscopy."
And
" Where the Truth Lies (pun intended) {Und}
Good microscopy adheres to a philosophical base, one not easily perceived on the surface, and this increases variability by permitting alternative interpretations. From this standpoint, I would suggest that science and the public at large are more concerned with the reduction of variability, i.e., better precision, rather than the pursuit of truth, i.e., accuracy. The trend toward reduction in variability is clearly seen in the ISO 9000 and 14000 standards that use the criteria of conformance with regard to consistent production (variability reduction) and not necessarily the production of a good product. This focus tends to ostracize microscopists and promote microscope users."
Just some food for thought.
Tony
ps One could state the same for the term Scientist
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
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-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Wednesday, April 30, 2008 11:43 AM To: ph2-at-sprynet.com
Jacques, What I've always loved about SEMs is that they are not microscopes but a signal generator and a signal processor. Tell me what you want to see and I can probably show it to you. Then we can sit down and have a long talk about whether or not it's real. This applies several times over to EDS. "But the computer said..." I really don't care what the computer said. If you don't understand the equipment, the physics of its signal generation and the many artifacts that can be generated, then you have no business putting out results that others, equally ignorant on the subject, are depending on. Sometimes lives depend on the results. Are we willing to pay for skilled people? Sadly, often the answer is no. Should we pay for skilled people? Absolutely yes.
The high level of automation has created a monster in more areas than just microanalysis. If it's easy to get pretty pictures and quantitative results to several decimal places, it must not require any skill, right? (and the quant results were off of a very rough fracture surface...must be accurate)
It seems everything from SEMs to the Wall Street Journal is being dumbed down. Sorry to be so pessimistic.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Wednesday, April 30, 2008 7:48 AM To: kenconverse-at-qualityimages.biz
Hi Steve, and lister
As other have pointed out, workload is not diminishing, but rather increasing.
But, what is changing, as I see, is the way microscopy is done. One think effectively more and more than one don't need a "microscopist"; than microscopes, as they work with a computers are just push button / click mouse devices. The origin is as much in the increasing workload, which need more time spent on the microscope, more than one man can give, as in the way some instruments are built, given the impression that "microsopist" is not a craft/trade ("métier" what's the adequate word in english ?). Everyone is able to do microscopy...
I'll give two illustrations :
First, all today EDS systems could be described, in an exagerate way, 5 fonctions : picture, acquire, ident, quant, report. The report gives results with two or three decimals, a total of 100.000 % etc... No need for a setup, nor for an analyste. Each fresh arrived student can do EDS,... and go back with poor results. But he/she doesn't kow how they are poor. There is no microanalyste anymore to explain him/her some basic rules. And he/she has so much to do trying to get results with other techniques like SEM, TEM, XRD, FTIR, Raman, SIMS, AES, VGT, AHHKB, RUIS, etc etc that he/she cannot learn everything.
Of coarse, I exagerate... but so fiew.
I see a second illustration in these new comming "desktop SEMs" about which there are much questions on the list. OK, I'll be the "vilain petit canard", the ugly duckling, which brings a false note in the music. But I would like to cartoon too these expensive toys : -take the best SEM you could build, -fix a price a bit higher than a good optical microscope and a bit lower than a low-end SEM -fix the profit you want to do, -simplify pitiless your SEM until it fits in the price -replace the maximum settings by auto functions -give the result to a designer, to create a fashoned look. You have now a click/mouse SEM with four functions : on, auto-focus, (auto)-mag, (auto)-capture. If you pay a bit more, the SEM will choose for your the right place of the sample and take a picture in full-auto mode at the best magnification, respecting the Imaging Gold Rules by the use of the new super build-in proprietary/patended WkEZY algorithm, etc... You don't need to think, buy and click !
An exageration, mmmmmhhh ? OK, I agree than there are specific domaines where such SEM may be useful, for exemple as field instruments, or for some routine observations. But there is much too noise about them, and I fear the main result they will produce is to enforce the false idee that one do not need a microscopist. Hey, a primary school child can now do SEM, why pay a microscopist !
Comming back to Steve's question, I must say that I understand it well. I have no fear for my job/position, but I see other places where things evoluate in the wrong way. The microscopist retire, is not replaced, everyone (or no-one, and the microscope is shut down) uses the microscope, and if one need it to do correct job, one have to clean, realigne, etc first. The results are missused equipement, poor or false results, which had could be right with the right settings, etc. One look after structurations in a polymere on pictures taken at 30 keV from a samples covered with 0.5 micron gold ! At the end, the methode itself can be discredited (EDS is not precise, is not accurate, etc. what have I soon heard). And it's not well accepted, if one have to say someone that he/she missused the methode. This point was evocated in a thread in the last weeks on the list.
Am I exagerating again ? Are other not confronted to such situations ?
Tell me I'm wrong, I've so the tendancy to be pessimistic ! ;-)
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
steven-fischer-at-hotmail.co.uk a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both steven-fischer-at-hotmail.co.uk as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: steven-fischer-at-hotmail.co.uk } Name: Steve } } Title-Subject: [Filtered] SEM and LM Future of Microscopy } } Question: Fellow microscopists } } I would like to open a new thread to discuss the future of } microscopy, applications and uses in problem solving etc. } } I use both a research grade light microscope and a high resolution } SEM (1.5kev to 30kev high vacuum) with elemental analysis capability. } The samples I look at are mostly materials based: problem solving, } research substances, contaminants etc as opposed to biological } samples. } } Are there any reasons to think that worldwide microscopy requirements } are likely to fall off (economically, scientifically or technology.) } } Are things headed towards a macroscopic view? } } What other applications are there for microscopy? } } Please be as detailed or succint as you wish. } } The reason why I ask is that I am in the unfortunate position where I } have to justify my job as a microscopist and would like to call upon } the community to help with ideas to counter a redundancy. } } Many thanks. } } } Login Host: 85.12.64.148 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 14, 27 -- From zaluzec-at-microscopy.com Sat Apr 26 08:08:29 2008 } 14, 27 -- Received: from sodium.colo.stayonline.net (neon.colo.stayonline.net [69.25.86.37]) } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3QD8T3c011105 } 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 08:08:29 -0500 } 14, 27 -- X-ASG-Debug-ID: 1209215299-737e005c0001-4CH8be } 14, 27 -- X-Barracuda-URL: http://192.168.5.11:8000/cgi-bin/mark.cgi } 14, 27 -- Received: from [172.16.0.236] (unknown [12.170.146.2]) } 14, 27 -- by sodium.colo.stayonline.net (Spam Firewall) with ESMTP id 69222264B68 } 14, 27 -- for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 09:08:20 -0400 (EDT) } 14, 27 -- Received: from [172.16.0.236] ([12.170.146.2]) by sodium.colo.stayonline.net with ESMTP id 6irR7OUfBFTki9Iq for {microscopy-at-microscopy.com} ; Sat, 26 Apr 2008 09:08:20 -0400 (EDT) } 14, 27 -- Mime-Version: 1.0 } 14, 27 -- Message-Id: {p06240802c438d9babf80-at-[172.16.0.236]} } 14, 27 -- Date: Sat, 26 Apr 2008 08:08:25 -0500 } 14, 27 -- To: microscopy-at-microscopy.com } 14, 27 -- From: steven-fischer-at-hotmail.co.uk (by way of MicroscopyListserver) } 14, 27 -- X-ASG-Orig-Subj: viaWWW: SEM and LM Future of Microscopy } 14, 27 -- Subject: viaWWW: SEM and LM Future of Microscopy } 14, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 14, 27 -- X-Barracuda-Connect: UNKNOWN[12.170.146.2] } 14, 27 -- X-Barracuda-Start-Time: 1209215300 } 14, 27 -- X-Barracuda-Spam-Score: 0.10 } 14, 27 -- X-Barracuda-Spam-Status: No, SCORE=0.10 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests=RDNS_NONE } 14, 27 -- X-Barracuda-Spam-Report: Code version 3.1, rules version 3.1.48892 } 14, 27 -- Rule breakdown below } 14, 27 -- pts rule name description } 14, 27 -- ---- ---------------------- -------------------------------------------------- } 14, 27 -- 0.10 RDNS_NONE Delivered to trusted network by a host with no rDNS } ==============================End of - Headers============================== }
==============================Original Headers============================== 19, 31 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Apr 30 06:44:27 2008 19, 31 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 19, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UBiQoD030653 19, 31 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 06:44:26 -0500 19, 31 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 19, 31 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m3UBiNIM023731 19, 31 -- ; Wed, 30 Apr 2008 13:44:23 +0200 (CEST) 19, 31 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 19, 31 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 2F24710876B1; 19, 31 -- Wed, 30 Apr 2008 13:43:45 +0200 (CEST) 19, 31 -- Message-ID: {48185B70.90302-at-ipcms.u-strasbg.fr} 19, 31 -- Date: Wed, 30 Apr 2008 13:43:44 +0200 19, 31 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 19, 31 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080306) 19, 31 -- MIME-Version: 1.0 19, 31 -- To: steven-fischer-at-hotmail.co.uk, Microscopy-at-microscopy.com 19, 31 -- Subject: Re: [Microscopy] viaWWW: Future of "the Microscopist" and some related 19, 31 -- comments about desktop SEMs 19, 31 -- References: {200804261317.m3QDHruL019550-at-ns.microscopy.com} 19, 31 -- In-Reply-To: {200804261317.m3QDHruL019550-at-ns.microscopy.com} 19, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 31 -- Content-Transfer-Encoding: 8bit 19, 31 -- X-IPCMS-MailScanner: Found to be clean 19, 31 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 19, 31 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.152]); Wed, 30 Apr 2008 13:44:23 +0200 (CEST) 19, 31 -- X-Virus-Scanned: ClamAV 0.93/6668/Tue Apr 8 13:57:49 2008 on mr2.u-strasbg.fr 19, 31 -- X-Virus-Status: Clean 19, 31 -- X-Spam-Status: No, score=1.5 required=5.0 tests=IP_LINK_PLUS,NORMAL_HTTP_TO_IP, 19, 31 -- WEIRD_PORT autolearn=disabled version=3.2.4 19, 31 -- X-Spam-Level: * 19, 31 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr2.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 33, 26 -- From kenconverse-at-qualityimages.biz Wed Apr 30 10:38:26 2008 33, 26 -- Received: from mta15.adelphia.net (mta15.adelphia.net [68.168.78.77]) 33, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m3UFcPCd005799 33, 26 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 30 Apr 2008 10:38:25 -0500 33, 26 -- Received: from Ken ([72.227.100.25]) by mta15.adelphia.net 33, 26 -- (InterMail vM.6.01.05.04 201-2131-123-105-20051025) with ESMTP 33, 26 -- id {20080430153753.FUBM29625.mta15.adelphia.net-at-Ken} ; 33, 26 -- Wed, 30 Apr 2008 11:37:53 -0400 33, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 33, 26 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr} , 33, 26 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 33, 26 -- Subject: RE: [Microscopy] Re: viaWWW: Future of "the Microscopist" and some related 33, 26 -- Date: Wed, 30 Apr 2008 11:38:12 -0400 33, 26 -- Message-ID: {000501c8aad8$3390bac0$6401a8c0-at-Ken} 33, 26 -- MIME-Version: 1.0 33, 26 -- Content-Type: text/plain; 33, 26 -- charset="iso-8859-1" 33, 26 -- X-Priority: 3 (Normal) 33, 26 -- X-MSMail-Priority: Normal 33, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 33, 26 -- Importance: Normal 33, 26 -- Thread-Index: AciquAEl+0z3qpf+TImX6VhYUCzOAgAHW5cg 33, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 33, 26 -- In-Reply-To: {200804301147.m3UBlkY6002421-at-ns.microscopy.com} 33, 26 -- Content-Transfer-Encoding: 8bit 33, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m3UFcPCd005799 ==============================End of - Headers==============================
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Seems to me that there is now a very diverse range of instruments with imaging as well as analysis capacity, all with digital user interface of some sort. Familiarity with computers means novices see a familiar type of interface, and these days it means that almost every conceivable instrument parameter is saved for every image/dataset collected.
However, as Ken points out, this also means that complex instrumentation tends to get treated like any old office desktop.
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Email: mcintyre-at-optics.rochester.edu Name: Brian McIntyre
Organization: Univ of Rochester
Title-Subject: [Filtered] Electron Microscopy class projects
Question: Hi all-
'Tis the time when my students must post a web version of their class projects. There are few, but some very good, projects this year. Please take a minute to look and offer comments.
} } Forbidden } } } } You don't have permission to access } http://www.seas.rochester.edu/cgi-bin/FormMail.pl on this server.
For this one:
} These specimens seem to be of large dimensions--not sub-micron, or } certainly not 250nm or less. Direct write E-beam is for the lowest } layers at say 120nm, 90nm or 65nm. How does this work follow suit } and how does it complement or replace OPC?
gary g.
At 04:43 PM 4/30/2008, you wrote:
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==============================Original Headers============================== 13, 20 -- From gary-at-gaugler.com Wed Apr 30 20:06:58 2008 13, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4116wDX026555 13, 20 -- for {microscopy-at-microscopy.com} ; Wed, 30 Apr 2008 20:06:58 -0500 13, 20 -- Message-Id: {200805010106.m4116wDX026555-at-ns.microscopy.com} 13, 20 -- Received: (qmail 21041 invoked from network); 30 Apr 2008 18:06:57 -0700 13, 20 -- Received: by simscan 1.1.0 ppid: 21036, pid: 21037, t: 0.0776s 13, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 13, 20 -- by smtp2 with SMTP; 30 Apr 2008 18:06:57 -0700 13, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 20 -- Date: Wed, 30 Apr 2008 18:08:59 -0700 13, 20 -- To: mcintyre-at-optics.rochester.edu 13, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 20 -- Subject: Re: [Microscopy] viaWWW: Electron Microscopy class projects 13, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 20 -- In-Reply-To: {200804302343.m3UNhKZV013608-at-ns.microscopy.com} 13, 20 -- References: {200804302343.m3UNhKZV013608-at-ns.microscopy.com} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-35F711E3 ==============================End of - Headers==============================
I'm looking for an image of a TEM/SEM pole piece contaminated with naoparticles / magnetic pieces etc. I would like to use such an image to educate my customers. Image(s) will tell a very clear story. Sometimes words are not enough.
Kind Regards, Ayten Celik-Aktas, PhD
==============================Original Headers============================== 3, 27 -- From celikaktas-at-gmail.com Thu May 1 05:24:14 2008 3, 27 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.187]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m41AO8kn026519 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 May 2008 05:24:11 -0500 3, 27 -- Received: by fk-out-0910.google.com with SMTP id z23so602518fkz.2 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 01 May 2008 03:24:07 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=IS52YdRU2PUYz7VRM7kuwRmPOCg0jUuttRzFE03FFGs=; 3, 27 -- b=EFwdnDBBr76EluXj/uD/kIiBr11OhB3w5EpDVfOfJZRDoqtvKDkjA3vZABKCsFCjAa9YRB9ra+I6T9XKkqnwCeUBR1eZZSckNetXbuR8/ZwIIcK/d4GqhLfIf3wHMotvMOvg77J7UPCbJX82uTzwJjAscRJMH8DQmDr0dDhCoWg= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=iVgxcYpM35GF+YyitPdF05pS1b8BfCrJOP1gHGqiAwDXF1LLJNZOdH8mRdqhjQ/zLZry9/5A+dClOCZvOrBS0jCpJKwi5UcCo5j0PdisjtD5t0P00UKf6SHb9WO1cX3a8NW7rY98anajuCpzor/AKovjY7Vk8itS0aO1pubkj2Y= 3, 27 -- Received: by 10.82.145.7 with SMTP id s7mr223874bud.81.1209637447583; 3, 27 -- Thu, 01 May 2008 03:24:07 -0700 (PDT) 3, 27 -- Received: by 10.82.126.2 with HTTP; Thu, 1 May 2008 03:24:07 -0700 (PDT) 3, 27 -- Message-ID: {1075c5c10805010324l4b2bff7bi748da284979d6531-at-mail.gmail.com} 3, 27 -- Date: Thu, 1 May 2008 13:24:07 +0300 3, 27 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} 3, 27 -- To: microscopy {microscopy-at-microscopy.com} 3, 27 -- Subject: image of contaminated pole piece 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=UTF-8 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] problems with plant tissue for immuno-TEM
Question: Does anyone have experience or advice concerning the use of epoxy resins such as Quetol or Spurr's for on-section antibody labeling in general and with plant leaf tissue specifically? We have experienced problems using acrylic resins such as LR White and Lowicryl with leaf tissue. The main issue seems to be poor infiltration of the resin, which results in blocks that produce holes around and throughout the tissue in the sections. A variety of approaches have been tried with the acrylics but with only marginal success, so we are considering an epoxy as a possible alternative. Any suggestions or insights are greatly appreciated.
Project MICRO's online database of books, videos, CDs, DVDs, and websites has been revised again, and it's worth a visit. It's intended to support MICRO (MSA's middle school outreach program) - but you'll find items that will interest all of you - even if you're an old mossback like me. DVD is a new category, and now you can search for books in Spanish (half a dozen good ones). Try a search by publication year if you want to see what is new. There's even a book of poetry about microorganisms that Nobel Laureate Arthur Kornberg wrote for his grandchildren; it's illustrated with light and electron micrographs, and the poems even rhyme!
See the URL below. -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America Box 117, 45301 Caspar Point Road Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 2, 21 -- From schooley-at-mcn.org Thu May 1 17:52:43 2008 2, 21 -- Received: from dns4.mcn.org (dns4.mcn.org [216.150.240.31]) 2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m41Mqgeh010955 2, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 1 May 2008 17:52:42 -0500 2, 21 -- Received: from [66.81.74.84] 2, 21 -- by dns4.mcn.org with esmtpa (Exim 4.69) 2, 21 -- (envelope-from {schooley-at-mcn.org} ) 2, 21 -- id 1JrhdA-0002RT-6I; Thu, 01 May 2008 15:52:01 -0700 2, 21 -- Mime-Version: 1.0 2, 21 -- Message-Id: {a06200700c43ff3bc9f23-at-[66.81.75.37]} 2, 21 -- Date: Thu, 1 May 2008 15:52:19 -0700 2, 21 -- To: Microscopy-at-MSA.Microscopy.Com 2, 21 -- From: Caroline Schooley {schooley-at-mcn.org} 2, 21 -- Subject: Microscopy education 2, 21 -- Cc: ech-at-interchange.ubc.ca, jjpayne-at-mmm.com, pfungosakR-at-aol.com, 2, 21 -- douglas.taatjes-at-uvm.edu, gwc-at-u.arizona.edu, nev-at-ccmr.cornell.edu, 2, 21 -- paige-johnson-at-utulsa.edu, sbarlow-at-sunstroke.sdsu.edu, 2, 21 -- seraphin-at-u.arizona.edu, alice.dohnalkova-at-pnl.gov, dkoleary-at-verizon.net, 2, 21 -- JeanDP-at-aol.com, mccanns-at-tiac.net, lesley.bechtold-at-jax.org, 2, 21 -- msi-at-soe.ucsc.edu, rosslm-at-missouri.edu 2, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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We have old Philips SEM 525, which we would like to get rid off. This microscope was completely operational a year ago, but was not in use since that time. If you have any interest, please contact me directly.
If anyone out there knows of, owns or has bits from a Mk1 or Mk2 Jeol 1200 EX TEM that they are thinking about parting with then let me know with the terms. It doesn't mater where in the world and in what condition. It all maybe of interest. As we have one that needs a few bits to get ours up and running perfectly and would welcome the parts. If anyone happens to have a quick release multi specimen rod knocking around in the back of the cabinet that would be immediately appreciated.
Regards John
==============================Original Headers============================== 3, 27 -- From john.mitchels-at-gmail.com Fri May 2 04:00:16 2008 3, 27 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.234]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4290F2Q020296 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 2 May 2008 04:00:15 -0500 3, 27 -- Received: by wx-out-0506.google.com with SMTP id h30so1493854wxd.21 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 02 May 2008 02:00:15 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=94YcqMDWLB26sOeiv2C/ZEoG3U4eCOeKTUqpCgELpWM=; 3, 27 -- b=ZABdWdzr7ul7G8ru6Ujqtg77CFlBCU9m2W6HGJ4rwnrPjSnqf0G4KMDY+qeWDKL2eiDl3SWIxQsFAlCM9KCwXsN3YmHNwTe5wsq19kgVhieaJmLhEqCJ/Wrb2kIsbT3SjvSwuiNlcqDpFHI61if7k29Vsy8Q3j2FFsyT57AthJ0= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=NqF8r9DjFT8/6JMr+VGIhFu7fq+FR7Fg+a/QLAP6gl+3rsaYnklYZyEP6Gz1VTAZN9YWUjN4+fwEE9971FUQEp1a9UA6jbNCVUEf1rHUb6d5D4232zxG9dYzvJmcRtkR+TQLW/PmfeoE8DR0F79xIO8Pm/jvVlrqo4RXe9FYpPM= 3, 27 -- Received: by 10.90.49.3 with SMTP id w3mr4363611agw.106.1209718815052; 3, 27 -- Fri, 02 May 2008 02:00:15 -0700 (PDT) 3, 27 -- Received: by 10.90.90.17 with HTTP; Fri, 2 May 2008 02:00:14 -0700 (PDT) 3, 27 -- Message-ID: {1b3cf7c30805020200n6ade2213hb976baf7612771fa-at-mail.gmail.com} 3, 27 -- Date: Fri, 2 May 2008 10:00:14 +0100 3, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: TEM MK1/2 JEOL 1200EX 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Hi David, Experiment...some antibodies work fine with Spurr's embedded samples and others do not. Sometimes increasing the amount of NaCl in your buffer (0.5M NaCl) helps.
Let me know if you want a protocol. best, Beth
************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
*************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
----- Original Message ----- X-from: dlowry-at-asu.edu To: beth-at-plantbio.uga.edu Sent: Thu, 01 May 2008 08:08:17 -0400
David, Another option is to try butyl methyl methacrylate. Although this resin is optimal for light level work, it is usuable in TEM. You just need to be a little careful about beam damage (the resin has no crosslinker and so it is a bit soft in the beam). It also will not preserve ultrastructure as crisply as epoxy or even LRwhite, but it has excellent immuno props and it infiltrates just fine. I can also send you a reference where it was used for immunoTEM (albeit not on leaves).
As ever, Tobias } } Hi David, } Experiment...some antibodies work fine with Spurr's embedded samples } and others do not. Sometimes increasing the amount of NaCl in your } buffer (0.5M NaCl) helps. } } Let me know if you want a protocol. } best, } Beth } } } ************************************** } Beth Richardson } Electron Microscopy Lab Coordinator } Plant Biology Department } University of Georgia } Athens, GA 30602-7271 } } http://www.plantbio.uga.edu/emlab/ } } Phone - (706) 542-1790 & FAX - (706) 542-1805 } } } } } ----- Original Message ----- } X-from: dlowry-at-asu.edu } To: } beth-at-plantbio.uga.edu } Sent: Thu, 01 May 2008 08:08:17 -0400 } Subject: } [Microscopy] viaWWW: problems with plant tissue for immuno-TEM } } } } } } } } } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: dlowry-at-asu.edu } } Name: David Lowry } } } } Organization: Arizona State University } } } } Title-Subject: [Filtered] problems with plant tissue for immuno-TEM } } } } Question: Does anyone have experience or advice concerning the use of } } epoxy resins such as Quetol or Spurr's for on-section antibody } } labeling in general and with plant leaf tissue specifically? We have } } experienced problems using acrylic resins such as LR White and } } Lowicryl with leaf tissue. The main issue seems to be poor } } infiltration of the resin, which results in blocks that produce holes } } around and throughout the tissue in the sections. A variety of } } approaches have been tried with the acrylics but with only marginal } } success, so we are considering an epoxy as a possible alternative. } } Any suggestions or insights are greatly appreciated. } } } } } } } }
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Email: yglukhoy-at-mectech.com Name: yuri glukhoy
Organization: MeC Technology, Inc
Title-Subject: [Filtered] scanning electron microscope
Question: Who is developing an ion beam system for etching of samples inside of the chamber of SEM? Yuri Glukhoy, PhD Chief Scientist MEC Tech, Inc. 2200 Industrial Way South Toms River, NJ 08755 (732) 505-0308 ext 353 yglukhoy-at-mectech.com http: / www.mectech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zerfasp-at-ors.od.nih.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] slightly used microscope
Question: Dear Microscopist, Does anyone have a slightly used TEM microscope for sale. I was hoping to purhase a used microscope with about 15+ years of serviceable life. Preferably the EM is located in the USA or Canada for easy transportation.
You could get a Gatan 682(?) and etch outside the chamber or get a twin beam system that does ion and e-beam. Probably $30K for Gatan and $500K for twin beam.
Another option is to etch using the etch mode of a sputter coater. Depends on what your end use is.
No financial interest.
gary g.
At 04:17 PM 5/2/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 21 -- From gary-at-gaugler.com Fri May 2 18:22:40 2008 7, 21 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m42NMd3s004855 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 May 2008 18:22:39 -0500 7, 21 -- Message-Id: {200805022322.m42NMd3s004855-at-ns.microscopy.com} 7, 21 -- Received: (qmail 22266 invoked from network); 2 May 2008 16:22:36 -0700 7, 21 -- Received: by simscan 1.1.0 ppid: 22263, pid: 22264, t: 0.1222s 7, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 21 -- by smtp1 with SMTP; 2 May 2008 16:22:36 -0700 7, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 21 -- Date: Fri, 02 May 2008 16:24:36 -0700 7, 21 -- To: yglukhoy-at-mectech.com 7, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 21 -- Subject: Re: [Microscopy] viaWWW: ion beam system for etching of 7, 21 -- samples inside of the 7, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 21 -- In-Reply-To: {200805022317.m42NH3xq028354-at-ns.microscopy.com} 7, 21 -- References: {200805022317.m42NH3xq028354-at-ns.microscopy.com} 7, 21 -- Mime-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-2BC4641B ==============================End of - Headers==============================
Yuri, We manufacture and sell the IBS/e ion beam sputter and etch system for SEM for ion polishing and etching samples ex-situ from the SEM. Baltec manufactures an ion milling system for TEM preparation that has an SEM column on it. And of course there are dual beam FIB systems that Gary mentioned.
You could easily put an ion gun into an SEM system. There are ion gun kits from several manufactures, we or one of our competitors could probably sell you a gun. However, if you do that you need to consider what you are getting into. Have you ever looked inside a ion sputter coating system? You will sputter material all over your SEM. It will coat all surfaces. The distribution of species from a surface from an ion beam has a cosine distribution. You will coat any detector with material, you will coat over insulators and possibly short out signal lines and control lines. Any rubbing surface that may have had a dry lubricant will now have a non-lubricious coating that could cause problems with moving parts in the stage. In short, the addition of an ion beam to an SEM is probably not a good idea without major considerations in the design.
Now, having said that, there are solutions that allow you to move samples from one system to another either under vacuum or under protective atmospheres. You could adapt what is already available or design your own. You could also design a load lock system for you SEM that would have an ion gun in place. If I were to do it, this is probably the route that I would take.
I hope this helps.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: yglukhoy-at-mectech.com [mailto:yglukhoy-at-mectech.com] Sent: Friday, May 02, 2008 4:19 PM To: Walck-at-SouthBayTech.com
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Email: yglukhoy-at-mectech.com Name: yuri glukhoy
Organization: MeC Technology, Inc
Title-Subject: [Filtered] scanning electron microscope
Question: Who is developing an ion beam system for etching of samples inside of the chamber of SEM? Yuri Glukhoy, PhD Chief Scientist MEC Tech, Inc. 2200 Industrial Way South Toms River, NJ 08755 (732) 505-0308 ext 353 yglukhoy-at-mectech.com http: / www.mectech.com
Scott; Robert Jamison and I built an ion gun into an SEM as described in:
Combining an Argon Ion Mill and a Field Emission Scanning Electron Microscope for Ultra High Precision Endpoint Detection in Preparing TEM Cross-Sections of Micro-Electronic Devices, with R. Jamison, Proceedings, 14 International Congress on Electron Microscopy, 1998, p. 403.
A huge problem we had was contamination of the apertures by the sputtered material. Yes, the gunk went up the pole piece and deposited non-conducting material on the apertures resulting in uncontrollable astigmatism. A lollipop shaped shield had to be inserted over the end of the lens to protect the apertures during milling.
John Mardinly, Numonyx
-----Original Message----- X-from: walck-at-southbaytech.com [mailto:walck-at-southbaytech.com] Sent: Friday, May 02, 2008 7:12 PM To: MARDINLY, A
Yuri, We manufacture and sell the IBS/e ion beam sputter and etch system for SEM for ion polishing and etching samples ex-situ from the SEM. Baltec manufactures an ion milling system for TEM preparation that has an SEM column on it. And of course there are dual beam FIB systems that Gary mentioned.
You could easily put an ion gun into an SEM system. There are ion gun kits from several manufactures, we or one of our competitors could probably sell you a gun. However, if you do that you need to consider what you are getting into. Have you ever looked inside a ion sputter coating system? You will sputter material all over your SEM. It will coat all surfaces. The distribution of species from a surface from an ion beam has a cosine distribution. You will coat any detector with material, you will coat over insulators and possibly short out signal lines and control lines. Any rubbing surface that may have had a dry lubricant will now have a non-lubricious coating that could cause problems with moving parts in the stage. In short, the addition of an ion beam to an SEM is probably not a good idea without major considerations in the design.
Now, having said that, there are solutions that allow you to move samples from one system to another either under vacuum or under protective atmospheres. You could adapt what is already available or design your own. You could also design a load lock system for you SEM that would have an ion gun in place. If I were to do it, this is probably the route that I would take.
I hope this helps.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: yglukhoy-at-mectech.com [mailto:yglukhoy-at-mectech.com] Sent: Friday, May 02, 2008 4:19 PM To: Walck-at-SouthBayTech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both yglukhoy-at-mectech.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: yglukhoy-at-mectech.com Name: yuri glukhoy
Organization: MeC Technology, Inc
Title-Subject: [Filtered] scanning electron microscope
Question: Who is developing an ion beam system for etching of samples inside of the chamber of SEM? Yuri Glukhoy, PhD Chief Scientist MEC Tech, Inc. 2200 Industrial Way South Toms River, NJ 08755 (732) 505-0308 ext 353 yglukhoy-at-mectech.com http: / www.mectech.com
This firm is a great place to find high end scientific equipment including electron microscopes though I have never actually purchased an item from them. I have no financial interest in the firm but appreciate their monthly email listings.
Larry
zerfasp-at-ors.od.nih.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both zerfasp-at-ors.od.nih.gov as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: zerfasp-at-ors.od.nih.gov } Name: Patricia Zerfas } } Title-Subject: [Filtered] slightly used microscope } } Question: Dear Microscopist, } Does anyone have a slightly used TEM microscope for sale. I was } hoping to purhase a used microscope with about 15+ years of } serviceable life. Preferably the EM is located in the USA or Canada } for easy transportation. } } Thanks, } Patricia Zerfas } } Login Host: 128.231.88.7 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Fri May 2 18:14:47 2008 } 6, 11 -- Received: from [172.16.10.134] (msdvpn8.msd.anl.gov [130.202.238.72]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m42NEjOe023897 } 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 2 May 2008 18:14:47 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240801c44150d48b83-at-[172.16.10.134]} } 6, 11 -- Date: Fri, 2 May 2008 18:14:47 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: zerfasp-at-ors.od.nih.gov (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: slightly used microscope } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 10, 28 -- From Larry.Ackerman-at-ucsf.edu Mon May 5 12:21:22 2008 10, 28 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m45HLJOc008083 10, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 5 May 2008 12:21:21 -0500 10, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 10, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 10, 28 -- Mon, 05 May 2008 10:21:01 -0700 10, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 10, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 10, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Mon, 5 May 2008 10:21:12 -0700 10, 28 -- Message-ID: {481F41FE.5060303-at-ucsf.edu} 10, 28 -- Date: Mon, 05 May 2008 10:21:02 -0700 10, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 10, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 10, 28 -- Organization: UCSF, NeuroAnatomy 10, 28 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 10, 28 -- MIME-Version: 1.0 10, 28 -- To: zerfasp-at-ors.od.nih.gov, Microscopy-at-microscopy.com 10, 28 -- Subject: Re: [Microscopy] viaWWW: slightly used microscope 10, 28 -- References: {200805022327.m42NRrqt018768-at-ns.microscopy.com} 10, 28 -- In-Reply-To: {200805022327.m42NRrqt018768-at-ns.microscopy.com} 10, 28 -- X-OriginalArrivalTime: 05 May 2008 17:21:12.0095 (UTC) 10, 28 -- FILETIME=[6A5EB2F0:01C8AED4] 10, 28 -- X-WSS-ID: 64019E7729O12656667-01-01 10, 28 -- Content-Type: text/plain; 10, 28 -- charset=iso-8859-1; 10, 28 -- format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Here is the May 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Friday May 9, 2008.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor ========================= Looking at Proteins Interacting and Folding inside Cells Stephen W. Carmichael, Mayo Clinic
Life In and Under the Antarctic Ice Sheets Shawn Doyle, Pierre Amato and Brent Christner Louisiana State University
Identifying Foreign Material Contamination in Food and Food Ingredients V. L. St. Jeor, A. Lape, A. R. Muroski, C. McGuire, J.S. Kruger, and D. L. Elmore, Cargill Incorporated, Excelsior, MN
Development of TV-rate CCD Cameras for in-situ Electron Microscopy Lancy Tsung, Bill Mollon, Ming Pan, Yan Jia, Paul Mooney, and Chengye Mao, Gatan, Inc. Pleasanton, CA
Signature Analysis Applied to EDS Microanalysis J.W. Colby & D. C. Ward, xk, Incorporated, Clackamas, OR
Automated Image Acquisition of Polymer Blend Morphology in an SEM C. S. Todd1, J. Blackson1, G. Bar2, E. Garcia-Meitin3, D. Reuschle3 M. Janus4, M. Darus5 and A. Nickles5 , Dow Chemical, 1Midland, MI, 2Schkopau, Germany, 3Freeport, TX. FEI Company, 4Eindhoven, Netherlands, 5Hillsboro, OR
Surface Preparation of Uranium by Ion Milling Donald A. Carpenter and Robert L. Bridges, Y-12 National Security Complex, Oak Ridge, TN
Using Iodine Vapour Staining to Visualize Starch Distribution in a Dry, Extruded Food Jacquie Bond1 and Allan K Hardacre2 ,1 Scion Research Ltd, Rotorua, 2 New Zealand Institute for Crop & Food Research Ltd, Palmerston North, New Zealand
A Method to Characterize and Correct Elliptical Distortion in Electron Diffraction Patterns Vincent D.-H. Hou and Du Li, Micron Technology, Inc., Boise, ID
Insect Epicuticular Grease Visualised by Scanning Probe Microscopy Stanislav Gorb, Dagmar Voigt, Henrik Peisker, Max-Planck Institute for Metals Research, Stuttgart, Germany
Functional Collaborative Remote Microscopy: Inter-Continental Atomic Resolution Imaging J .M. Perkins,* D. A. Blom,*** D. W. McComb,* and L. F. Allard**, *Imperial College London, UK, ** Oak Ridge National Laboratory, Oak Ridge, TN, ***University of South Carolina, Columbia, SC
Why is it Difficult to Simulate EBSD Patterns Accurately? Alwyn Eades and Andrew Deal, Lehigh University, Bethlehem, PA
Quick Sample Preparation and EFTEM Elemental Characterization of FAB Based Defects C.T. Schamp, B.T. Valdez, J. Gazda, Cerium Labs, Austin, TX
Industry News
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==============================Original Headers============================== 21, 17 -- From randerson20-at-tampabay.rr.com Mon May 5 14:13:12 2008 21, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 21, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m45JDBEV018413 21, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 5 May 2008 14:13:11 -0500 21, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 21, 17 -- by hrndva-omta03.mail.rr.com with ESMTP 21, 17 -- id {20080505191310.SGNX16750.hrndva-omta03.mail.rr.com-at-[127.0.0.1]} 21, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 5 May 2008 19:13:10 +0000 21, 17 -- Message-ID: {481F5C43.3010503-at-tampabay.rr.com} 21, 17 -- Date: Mon, 05 May 2008 15:13:07 -0400 21, 17 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 21, 17 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 21, 17 -- MIME-Version: 1.0 21, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 21, 17 -- Subject: May 2008 Microscopy Today Table of Contents 21, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
The Analytical Imaging Facility (www.aecom.yu.edu/aif), a component of the Gruss-Lipper Biophotonics Center, the Cancer Center and a core resource of the Albert Einstein College of Medicine, is seeking a Director of Light Microscopy. We seek a dynamic, motivated individual with expertise in current techniques for fluorescence imaging of cells and tissues If you are looking for the opportunity to work in a state of the art imaging facility with leading biomedical scientists you are encouraged to apply.
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**************************************************************************** Frank Macaluso, Administrative Director and Director of Electron Microscopy phone: 718-430-3547 Analytical Imaging Facility fax: 718-430-8996 Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu 1300 Morris Park Avenue http://www.aecom.yu.edu/aif/ Bronx, NY 10461 ****************************************************************************
==============================Original Headers============================== 6, 25 -- From macaluso-at-aecom.yu.edu Mon May 5 15:05:10 2008 6, 25 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m45K53lm024465 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2008 15:05:08 -0500 6, 25 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 6, 25 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 7E4E99F0084 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2008 15:45:31 -0400 (EDT) 6, 25 -- X-AuditID: 816201a0-a769ebb0000015ac-51-481f63db741f 6, 25 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 6, 25 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 3000D718002 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 5 May 2008 15:45:31 -0400 (EDT) 6, 25 -- Received: from aif1.aecom.yu.edu (aif1.aif.aecom.yu.edu [129.98.80.55]) 6, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 25 -- (No client certificate requested) 6, 25 -- by post.aecom.yu.edu (Postfix) with ESMTP id 0CEE15F; 6, 25 -- Mon, 5 May 2008 15:45:30 -0400 (EDT) 6, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 25 -- Date: Mon, 05 May 2008 15:46:47 -0400 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- From: Frank Macaluso {macaluso-at-aecom.yu.edu} 6, 25 -- Subject: Position Open, Director of Light Microscopy 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 25 -- Message-Id: {20080505194531.0CEE15F-at-post.aecom.yu.edu} 6, 25 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scott.streiker-at-udri.udayton.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scott.streiker-at-udri.udayton.edu Name: Scott Streiker
Organization: University of Dayton Research Institute
Title-Subject: [Filtered] Sample preparation short course(s)
Question: Can anyone recommend a short course,in USA, on applications of embedding techniques, cryo-sectioning, and/or ultrathin sectioning of polymer composite materials for TEM and SEM analysis? Thanks, Scott Streiker University of Dayton Research Institute Dayton, Ohio
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rboehrin-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rboehrin-at-vt.edu Name: Robert Boehringer
Organization: Va tech
Title-Subject: [Filtered] RE: viaWWW: Future of "the Microscopist" and some related
Question: The following comment really struck me as an essential issue.
"I see an increase in microscope users and a decrease in microscopists. The first uses comparisons to do science the other induction."
One of the interesting episodes of microscopy was the failure of textbooks for a period of 99 years to correctly describe the structure of the mammalian liver. This oversight was due to a misinterpretation, or more correctly an inductive mistake in determining the 3-d structure observed on slices under a microscope.
Although this was a spectacular error there have been others including descriptions of the structure of ER, glands in avian gut, mitochondria, and so forth.
There is also an inherent problem with doing comparisons. The quantification of histological information is often based on comparisons. This simple assumption that, "It's ok, I'm just comparing." is simply a bad decision. The underlying assumption is that comparisons are possible.
"I see more cells, there must be more cells" is a common comparison. This statement is not necessarily true! Sometimes it is true. Sometimes it is not. A good example of the condition in which it is not true is the assessment of cell numbers in the hippocampus across specimens of different ages. The differences seen in young and old specimens are not a result of changes in cell loss, or gain for that matter.
Relegating the microscope to a comparison tool is begging for the generation of gobs of incorrect results.
Mr. Steve Fischer, Short answer: The new technologies are easier to use than ever, but you still need an expert to make sure people are getting 2008 quality images from expensive microscope technologies instead of "good enough" images, which leave too many openings for misinterpretation and waste the quality available.
Are you in industry or academia? My background includes biological TEM for a dozen years in industry, followed by a return to academia. The industry job had me diversify to lighter microscopy areas due to less demand for the TEM technology.
The respect for electron microscopy has gone down with the evolution of other techniques of IHC, PCR, laser confocal microscopy, etc, but there will always be a need for the resolution which only EM provides. In addition, the degree of error which results from relying on the new technologies alone is starting to become realized. Every technology has its caveats and biases, so the take home lesson is to use more than one to support your data. We are finding that small lots of EM samples to support the other data has become valuable when competing for publications and grants. There will also always be situations that specifically require EM.
Regarding both EM and LM skills: I currently work for a department that had half-time individuals handling TEM and advance microscopy training and maintenance duties. Since I've been brought in full time, I've been told that the maintenance levels went down and the quality of work coming from the equipment went up. I also like to think that the data is coming out faster, since I'm answering user questions quickly and clearly.
We still don't use the whole value of our confocal microscopes, but I'm educating the researchers to start planning experiments that will.
Feel free to contact me off list if you want to ask questions about my opinions and experience.
Regards, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan Ann Arbor, Michigan USA
The 2 TEMs that we use are being upgraded for digital capture. Actually, one is being retrofitted with a camera very soon, the other will be replaced in the near future.
What software are people using for 3D reconstructions from TEM? Line tracing, surface rendering, segmentation and seed fill, EM tomography.....
We have Amira, and Neurolucida which we use with confocal images. Amira is heavily disliked due to its interface and learning curve, the Neurolucida much easier to use. We are familiar with Osirix, and use ImageJ a lot, but they are not really usable for reconconstructions from serial sections.
Thanks in advance,
Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 11, 24 -- From glenmac-at-u.washington.edu Tue May 6 17:25:52 2008 11, 24 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m46MPpN1015028 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 17:25:51 -0500 11, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.141] (may be forged)) 11, 24 -- by mxout1.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m46MPorJ008054 11, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 15:25:50 -0700 11, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 11, 24 -- (authenticated authid=glenmac) 11, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m46MPoqt031316 11, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 15:25:50 -0700 11, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 11, 24 -- Content-Transfer-Encoding: 7bit 11, 24 -- Message-Id: {B4433C26-A3D2-4BEE-9CEC-CAA8A77025B7-at-u.washington.edu} 11, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 11, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 11, 24 -- Subject: TEM 3D software 11, 24 -- Date: Tue, 6 May 2008 15:25:49 -0700 11, 24 -- X-Mailer: Apple Mail (2.753) 11, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.6.151235 11, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_1100_1199 0, BODY_SIZE_5000_LESS 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
To align the images I use AutoAligner from Bitplane (it is good for x,y & r). Line tracing is done in ImageJ. Segmentation of the data is done in Photoshop or in software I have had written. Seed Fill is done in my custom software (I would offer it, but it is very specific for what I do). 3D reconstruction and surface renderings are done in Volocity. Analysis of volumes, surface areas, shapes and locations are also done in Volocity.
Some of the methods (and many of the results) were published in Elliott et al, PNAS Feb 2008.
I guess I need to say it, I have no financial....
David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On May 6, 2008, at 4:24 PM, glenmac-at-u.washington.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } The 2 TEMs that we use are being upgraded for digital capture. } Actually, one is being retrofitted with a camera very soon, the other } will be replaced in the near future. } } What software are people using for 3D reconstructions from TEM? Line } tracing, surface rendering, segmentation and seed fill, EM } tomography..... } } We have Amira, and Neurolucida which we use with confocal images. } Amira is heavily disliked due to its interface and learning curve, } the Neurolucida much easier to use. We are familiar with Osirix, and } use ImageJ a lot, but they are not really usable for } reconconstructions from serial sections. } } Thanks in advance, } } Glen } } } } Glen MacDonald } Core for Communication Research } Virginia Merrill Bloedel Hearing Research Center } Box 357923 } University of Washington } Seattle, WA 98195-7923 USA } (206) 616-4156 } glenmac-at-u.washington.edu } } ********************************************************************** } ** } ****** } The box said "Requires WindowsXP or better", so I bought a Macintosh. } ********************************************************************** } ** } ****** } } } } ==============================Original } Headers============================== } 11, 24 -- From glenmac-at-u.washington.edu Tue May 6 17:25:52 2008 } 11, 24 -- Received: from mxout1.cac.washington.edu } (mxout1.cac.washington.edu [140.142.32.134]) } 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m46MPpN1015028 } 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 } 17:25:51 -0500 } 11, 24 -- Received: from smtp.washington.edu (smtp.washington.edu } [140.142.32.141] (may be forged)) } 11, 24 -- by mxout1.cac.washington.edu (8.13.7+UW06.06/8.13.7 } +UW07.09) with ESMTP id m46MPorJ008054 } 11, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=OK) } 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 } 15:25:50 -0700 } 11, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) } 11, 24 -- (authenticated authid=glenmac) } 11, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) } with ESMTP id m46MPoqt031316 } 11, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 } 15:25:50 -0700 } 11, 24 -- Mime-Version: 1.0 (Apple Message framework v753) } 11, 24 -- Content-Transfer-Encoding: 7bit } 11, 24 -- Message-Id: {B4433C26-A3D2-4BEE-9CEC- } CAA8A77025B7-at-u.washington.edu} } 11, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 11, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" } {microscopy-at-microscopy.com} } 11, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} } 11, 24 -- Subject: TEM 3D software } 11, 24 -- Date: Tue, 6 May 2008 15:25:49 -0700 } 11, 24 -- X-Mailer: Apple Mail (2.753) } 11, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: } 2.6.0.325393, Antispam-Data: 2008.5.6.151235 } 11, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, } Report='BODY_SIZE_1100_1199 0, BODY_SIZE_5000_LESS 0, __C230066_P5 } 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, } __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION } 0, __SANE_MSGID 0' } ==============================End of - } Headers============================== }
==============================Original Headers============================== 12, 22 -- From Elliott-at-arizona.edu Tue May 6 19:06:49 2008 12, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.Arizona.EDU [128.196.133.168]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4706maD025054 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 May 2008 19:06:48 -0500 12, 22 -- Received: from frodos_amavis (amavis1.email.arizona.edu [10.0.0.204]) 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 58206456E86 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 May 2008 17:06:47 -0700 (MST) 12, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 12, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7637F456DF4 12, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 6 May 2008 17:06:46 -0700 (MST) 12, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 12, 22 -- In-Reply-To: {200805062324.m46NO80f020902-at-ns.microscopy.com} 12, 22 -- References: {200805062324.m46NO80f020902-at-ns.microscopy.com} 12, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 22 -- Message-Id: {CC86E750-324A-491A-82E6-FF4AA3CB27EB-at-arizona.edu} 12, 22 -- Content-Transfer-Encoding: 7bit 12, 22 -- From: David Elliott {Elliott-at-arizona.edu} 12, 22 -- Subject: Re: [Microscopy] TEM 3D software 12, 22 -- Date: Tue, 6 May 2008 17:06:43 -0700 12, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 12, 22 -- X-Mailer: Apple Mail (2.753) 12, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Amira' power is well worth the learning curve. Their customer support used to be very good, too, - you an call and simply ask how to do something, if it is not on the manual. I found that making a quick 3D rendering from serial section works very nice in Amira. You have version 4.**, right?..
I notice IMOD is not in your list? You can trace easily using their 3Dmod module and get some really nice surface rendering.
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Begin forwarded message:
} From: glenmac-at-u.washington.edu } Date: May 6, 2008 6:45:09 PM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] TEM 3D software } Reply-To: glenmac-at-u.washington.edu } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } The 2 TEMs that we use are being upgraded for digital capture. } Actually, one is being retrofitted with a camera very soon, the other } will be replaced in the near future. } } What software are people using for 3D reconstructions from TEM? Line } tracing, surface rendering, segmentation and seed fill, EM } tomography..... } } We have Amira, and Neurolucida which we use with confocal images. } Amira is heavily disliked due to its interface and learning curve, } the Neurolucida much easier to use. We are familiar with Osirix, and } use ImageJ a lot, but they are not really usable for } reconconstructions from serial sections. } } Thanks in advance, } } Glen } } } } Glen MacDonald } Core for Communication Research } Virginia Merrill Bloedel Hearing Research Center } Box 357923 } University of Washington } Seattle, WA 98195-7923 USA } (206) 616-4156 } glenmac-at-u.washington.edu } } ********************************************************************** } ** } ****** } The box said "Requires WindowsXP or better", so I bought a Macintosh. } ********************************************************************** } ** } ****** } } } } ==============================Original } Headers============================== } 11, 24 -- From glenmac-at-u.washington.edu Tue May 6 17:25:52 2008 } 11, 24 -- Received: from mxout1.cac.washington.edu } (mxout1.cac.washington.edu [140.142.32.134]) } 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m46MPpN1015028 } 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 } 17:25:51 -0500 } 11, 24 -- Received: from smtp.washington.edu (smtp.washington.edu } [140.142.32.141] (may be forged)) } 11, 24 -- by mxout1.cac.washington.edu (8.13.7+UW06.06/8.13.7 } +UW07.09) with ESMTP id m46MPorJ008054 } 11, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=OK) } 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 } 15:25:50 -0700 } 11, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) } 11, 24 -- (authenticated authid=glenmac) } 11, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) } with ESMTP id m46MPoqt031316 } 11, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 11, 24 -- for {microscopy-at-microscopy.com} ; Tue, 6 May 2008 } 15:25:50 -0700 } 11, 24 -- Mime-Version: 1.0 (Apple Message framework v753) } 11, 24 -- Content-Transfer-Encoding: 7bit } 11, 24 -- Message-Id: {B4433C26-A3D2-4BEE-9CEC- } CAA8A77025B7-at-u.washington.edu} } 11, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 11, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" } {microscopy-at-microscopy.com} } 11, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} } 11, 24 -- Subject: TEM 3D software } 11, 24 -- Date: Tue, 6 May 2008 15:25:49 -0700 } 11, 24 -- X-Mailer: Apple Mail (2.753) } 11, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: } 2.6.0.325393, Antispam-Data: 2008.5.6.151235 } 11, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, } Report='BODY_SIZE_1100_1199 0, BODY_SIZE_5000_LESS 0, __C230066_P5 } 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, } __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION } 0, __SANE_MSGID 0' } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From vladislav_speransky-at-nih.gov Tue May 6 19:28:44 2008 8, 23 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m470SgLV027829 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 6 May 2008 19:28:42 -0500 8, 23 -- X-IronPortListener: NIH_Relay 8, 23 -- X-SBRS: None 8, 23 -- X-IronPort-AV: E=Sophos;i="4.27,445,1204520400"; 8, 23 -- d="scan'208";a="190670520" 8, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 8, 23 -- by nihrelayxway4.hub.nih.gov with ESMTP; 06 May 2008 20:27:45 -0400 8, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 8, 23 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m470SffL10950712 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 6 May 2008 20:28:41 -0400 (EDT) 8, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 8, 23 -- References: {200805062245.m46Mj9q9016945-at-ns.microscopy.com} 8, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 23 -- Message-Id: {8B4D9C74-A9A0-46F6-8115-48B0E08DCB8E-at-nih.gov} 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 23 -- Subject: Fwd: [Microscopy] TEM 3D software 8, 23 -- Date: Tue, 6 May 2008 20:28:07 -0400 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
Gatan's I-PREP system allows etching, polishing, or coating of a sample in an airlock attached to the SEM. It includes vacuum transfer into the SEM and LN cooling. http://www.gatan.com/sem/i-prep.php
Steve
========================================= Steven T. Coyle, Ph.D. Gatan Inc. 5794 W. Las Positas Blvd. Pleasanton, CA 94588 Direct (925) 224 7383 Fax (925) 463 0204 E-mail scoyle-at-gatan.com =========================================
-----Original Message----- X-from: yglukhoy-at-mectech.com [mailto:yglukhoy-at-mectech.com] Sent: Friday, May 02, 2008 4:28 PM To: scoyle-at-gatan.com
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Email: yglukhoy-at-mectech.com Name: yuri glukhoy
Organization: MeC Technology, Inc
Title-Subject: [Filtered] scanning electron microscope
Question: Who is developing an ion beam system for etching of samples inside of the chamber of SEM? Yuri Glukhoy, PhD Chief Scientist MEC Tech, Inc. 2200 Industrial Way South Toms River, NJ 08755 (732) 505-0308 ext 353 yglukhoy-at-mectech.com http: / www.mectech.com
As many will know I train microscopists around the world seeing what is happening to our topic and I agree totally with the comments in this mail.
We developed a paper on quality in electron microscopy laboratories particularly to point out that standards could do with improvement and offering a protocol to help. Unfortunately it is human nature to believe that we are all doing a good job!
As the number of instruments decline, seen world wide due to cost and other implications, our standards should increase with my thought that those who make no effort to improve standards should be the laboratories who die!
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {greggps-at-umich.edu} To: {protrain-at-emcourses.com} Sent: Tuesday, May 06, 2008 3:39 PM
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Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] Maximum lenght of vacuum lines
Question: All,
The oil mechanical vacuum pumps (a few each of belt drive and direct drive) for our 4 ea EM's are currently located in a service spline directly behind the instruments. Currently, the pump to scope distance is about 4-5 feet using rubber hose. Our safety folks are asking that we relocate the pumps. This will involve 1) moving them into the scope lab itself or into another room at the end of the hallway. I'm worried about noise and vibration if we relocate into the lab. If we put all the pumps into a single room it will involve long vacuuum line runs. Safety insists we will use hard tubing. The vacuum line runs could be up to 20 or more feet between the pumps and EM's. Also, the tubing run will involved 2 right angle bends. How far can I locate the pumps from scopes? Does it matter if the bends are mitered. Should I use larger diameter tubing than the current rubber hose?
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Email: s.miao-at-sheffield.ac.uk Name: shu miao
Title-Subject: [Filtered] Eucentric height and DV value on JEOL 2010
Question: Hello all, I got a question on setting the eucentric height on JEOL 2010. Normally, the eucentric height is determined by adjusting the z-position to minimize the sample's movement while tilting the sample around the x-axis. Once this is set, the sample can be focused by OBJ focus. When I do this on our JEOL 2010, however, the DV value associate with the OBJ focus is far away from 0 (the recommended value by engineer). This means the optimal OBJ focus plane is not same as the eucentric height. Based on your experience and knowledge, which one I should stick to, the encentric height or the DV=0? Personally, I think for high resolution work which has high requirement of OBJ lens performance, I should keep DV close to 0. Any comments are welcome.
Shu Miao Department of Engineering Materials University of Sheffield
Very simply the pumping speed is such that it follows the inverse square law. The further away the pump the slower the pump down. Bends in a pumping line slow the procedure even further.
When we design an instrument we expect the rotary pumps to be within 5 feet of the instrument and the facilities provided with the machine are designed to reduce vibration.
The only area that you may not like is the noise from the pump, however to use an acoustic cover is a far better solution that to have the pumps at the distance that you indicate.
Good luck
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {opmills-at-mtu.edu} To: {protrain-at-emcourses.com} Sent: Thursday, May 08, 2008 2:01 AM
What is the "safety" issue? Is this Brownian activity of a useless entity? Some units are good but others are not. Are they really doing something to make the situation better or are they just trying to justify their existence?
This safety stuff is really getting irritating and divisive.
gary g.
At 06:28 PM 5/7/2008, you wrote:
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==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Wed May 7 23:45:33 2008 7, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m484jTsP015077 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 7 May 2008 23:45:32 -0500 7, 20 -- Message-Id: {200805080445.m484jTsP015077-at-ns.microscopy.com} 7, 20 -- Received: (qmail 28375 invoked from network); 7 May 2008 21:43:14 -0700 7, 20 -- Received: by simscan 1.1.0 ppid: 28372, pid: 28373, t: 0.1168s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by qsmtp4 with SMTP; 7 May 2008 21:43:14 -0700 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Wed, 07 May 2008 21:45:26 -0700 7, 20 -- To: opmills-at-mtu.edu 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: Maximum lenght of vacuum lines 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200805080128.m481Sgvf018010-at-ns.microscopy.com} 7, 20 -- References: {200805080128.m481Sgvf018010-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-7B56590B ==============================End of - Headers==============================
protrain-at-emcourses.com wrote: } Very simply the pumping speed is such that it follows the inverse square } law.
Conductance of tubing is inversely proportional to tubing length, not inverse square. However, it is proportional to 4th power of diameter of tubing.
So, slight increase in diameter will compensate for the increased length. Doubling diameter would enable one to increase length 16 times for same conductance.
} The further away the pump the slower the pump down. Bends in a } pumping line slow the procedure even further.
Only if you use too small diameter tubing.
} When we design an instrument we expect the rotary pumps to be within 5 feet } of the instrument and the facilities provided with the machine are designed } to reduce vibration. } } The only area that you may not like is the noise from the pump, however to } use an acoustic cover is a far better solution that to have the pumps at the } distance that you indicate.
Well, not everyone agrees: http://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1001&context=physicsgay They make quite good points about their view..
For the original question: Simple solution is to increase diameter to compensate for length.
==============================Original Headers============================== 10, 19 -- From kristian.ukkonen-at-iki.fi Thu May 8 01:56:22 2008 10, 19 -- Received: from mail.mobnet.fi (mail.mobnet.fi [83.145.201.107]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m486uI9I000644 10, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2008 01:56:19 -0500 10, 19 -- Received: from [192.168.1.35] (unverified [83.145.228.24]) 10, 19 -- by mobnet-security.org (Mobnet Security - Farm 1) with ESMTP id 9695952-1801699 10, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 08 May 2008 09:56:20 +0300 10, 19 -- Message-ID: {4822A411.2090206-at-iki.fi} 10, 19 -- Date: Thu, 08 May 2008 09:56:17 +0300 10, 19 -- From: Kristian Ukkonen {kristian.ukkonen-at-iki.fi} 10, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 10, 19 -- X-Accept-Language: en-us, en 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Microscopy-at-microscopy.com 10, 19 -- Subject: Re: viaWWW: Maximum lenght of vacuum lines 10, 19 -- References: {200805080403.m4843V9l008195-at-ns.microscopy.com} 10, 19 -- In-Reply-To: {200805080403.m4843V9l008195-at-ns.microscopy.com} 10, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi to all! I am struggling with a professor because we have 2 different opinions on the following problem: We have a project involving the analysis of rat organs both in histology and in EM. The histology part only requires H&E staining, no special labeling and no immunofluorescence. No immunolabeling for EM neither. Well the histologists want the whole organs to be fixed for histology, then I could get a small piece for EM. No problem for me. But they want the organs fixed in formalin and I want them fixed in Karnovsky (Formaldehyde + glutaraldehyde). I wonder why it would not be possible to fix the whole organs in Karnovsky. Is Karnovsky fixation a problem for histology??! I know glutaraldehyde should not be used for IF, but we have no IF planned. I also know that glutaradehyde can lead to overfixation artifacts, but the material is being processed immediately and not intended to be stored for a long time. Another issue may be that histologists do not wish to work with cacodylate buffer, but they could simply change the fixation medium once I got my piece of tissue. I would appreciate your opinion and comments on the feasibility of using Karnovsky as fixation medium for histology. I wish you all have such a wonderful shiny day as we have here in Vienna. Best regards,
Stephane
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==============================Original Headers============================== 5, 21 -- From nizets2-at-yahoo.com Thu May 8 03:13:39 2008 5, 21 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m488DZjd010555 5, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 03:13:36 -0500 5, 21 -- Received: (qmail 60241 invoked by uid 60001); 8 May 2008 08:06:54 -0000 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 21 -- s=s1024; d=yahoo.com; 5, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 21 -- b=0a+ib7bGzn5UyLRM/aZxWE5q+QT38jnNV+rltJ3QXlB+d0bWHoATYCJWxU3Dbt8VeZDKsvMY4OX4nnmnbM1HbBADOm43gevaMlv4Nzo9JcTQEi/SPgABRTHzyDsZ6RfUSisLMbyESBvC0u/YX4t6bWWIv+n3m/E8QvcliGmgxU0=; 5, 21 -- X-YMail-OSG: e0OmUT0VM1mHQCDlFJwDqPHQv5no8a79H0g7x_MhbuZ2XESOrSD_Yo7EkEyneDWdKYoiHAsq.B5QPk0IkGkpUxNj_2k5V8p.fEfa5snAUAZAAfyQC_UnBEcE8EU- 5, 21 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Thu, 08 May 2008 01:06:54 PDT 5, 21 -- X-Mailer: YahooMailRC/975.23 YahooMailWebService/0.7.185 5, 21 -- Date: Thu, 8 May 2008 01:06:54 -0700 (PDT) 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 21 -- Subject: karnovsky and histology 5, 21 -- To: microscopy-at-microscopy.com 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 5, 21 -- Message-ID: {643729.60203.qm-at-web37406.mail.mud.yahoo.com} 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m488DZjd010555 ==============================End of - Headers==============================
Karnovsky's works fine for routine histology. It does not have to be used in cacodylate buffer. Phosphate works fine. Maunsbach and Afzelius, "Biomedical Electron Microscopy"* has excellent comparative micrographs of cacodylate and phosphate buffers. Your problem is less likely to be over-fixation and more likely to be under-fixation. Fixing of light-level histology tissue pieces really takes 24 to 48 hours**. The formalin penetrates quickly enough, it just doesn't fix that quickly. Glut penetrates more slowly, but fixes quicker when it gets in. Wouldn't help to fix first in formalin then change to Karnovsky's for your EM. The benefit of using glut would be gone.
Phil
*Excellent text. Every bio EM person should have this book. **Times for complete fixation in formalin is a common thread on the histonet mailing list. Usually grumping about how clinical turn-around times are too fast for proper/complete fixation.
} Hi to all! } I am struggling with a professor because we have } 2 different opinions on the following problem: } We have a project involving the analysis of rat } organs both in histology and in EM. The } histology part only requires H&E staining, no } special labeling and no immunofluorescence. } No immunolabeling for EM neither. } Well the histologists want the whole organs to } be fixed for histology, then I could get a small } piece for EM. No problem for me. But they want } the organs fixed in formalin and I want them } fixed in Karnovsky (Formaldehyde + } glutaraldehyde). I wonder why it would not be } possible to fix the whole organs in } Karnovsky.ÝIs Karnovsky fixation a problem for } histology??! } I know glutaraldehyde should not be used for IF, } but we have no IF planned. I also know that } glutaradehyde can lead to overfixation } artifacts, but the material is being processed } immediately and not intended to be stored for a } long time. Another issue may be that } histologists do not wish to work with cacodylate } buffer, but they could simply change the } fixation medium once I got my piece of tissue. } I would appreciate your opinion and comments on } the feasibility of using Karnovsky as fixation } medium for histology. } I wish you all have such a wonderful shiny day as we have here in Vienna. } Best regards, } } Stephane -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 25 -- From oshel1pe-at-cmich.edu Thu May 8 08:00:30 2008 6, 25 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48D0Sww009228 6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 8 May 2008 08:00:28 -0500 6, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 25 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m48DCx7G010809; 6, 25 -- Thu, 8 May 2008 09:13:06 -0400 6, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 6, 25 -- Thu, 8 May 2008 09:00:57 -0400 6, 25 -- Mime-Version: 1.0 6, 25 -- Message-Id: {f06240805c448a765a97d-at-[141.209.160.249]} 6, 25 -- In-Reply-To: {200805081151.m48Bp2tc001704-at-ns.microscopy.com} 6, 25 -- References: {200805081151.m48Bp2tc001704-at-ns.microscopy.com} 6, 25 -- Date: Thu, 8 May 2008 09:00:24 -0400 6, 25 -- To: nizets2-at-yahoo.com 6, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 25 -- Subject: Re: [Microscopy] karnovsky and histology 6, 25 -- Cc: Microscopy-at-microscopy.com 6, 25 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 25 -- X-OriginalArrivalTime: 08 May 2008 13:00:57.0765 (UTC) FILETIME=[8EBE4550:01C8B10B] 6, 25 -- X-CanItPRO-Stream: default 6, 25 -- X-Spam-Score: -4 () L_EXCH_MF 6, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m48D0Sww009228 ==============================End of - Headers==============================
Can anyone offer advice on how to make polishing suspensions for:
1 micron Alumina 0.05 micron Alumina
Is there a way to make these water based, but have someway to keep the particles in suspension for as long as possible?
Anyone have a recipe for making their own Colloidal Silica suspensions? Is so, where did you purchase the raw materials?
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 11, 19 -- From mmcheath-at-syr.edu Thu May 8 07:56:26 2008 11, 19 -- Received: from SUEXCL-02.ad.syr.edu (suexbe-02.ad.syr.edu [128.230.108.46]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48CuKuj008678 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 07:56:22 -0500 11, 19 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu ([128.230.108.49]) with Microsoft Exchange Server HTTP-DAV ; 11, 19 -- Thu, 8 May 2008 12:56:20 +0000 11, 19 -- User-Agent: Microsoft-Entourage/11.3.3.061214 11, 19 -- Date: Thu, 08 May 2008 08:56:18 -0400 11, 19 -- Subject: Recipes for making polishing suspensions 11, 19 -- From: Michael Cheatham {mmcheath-at-syr.edu} 11, 19 -- To: {microscopy-at-microscopy.com} 11, 19 -- Message-ID: {C44870B2.1CF0F%mmcheath-at-syr.edu} 11, 19 -- Thread-Topic: Recipes for making polishing suspensions 11, 19 -- Thread-Index: AcixCuf9Jl2bjBz+Ed2FPAAWy6AryQ== 11, 19 -- In-Reply-To: {200804101304.m3AD4IrA007515-at-ns.microscopy.com} 11, 19 -- Mime-version: 1.0 11, 19 -- Content-type: text/plain; 11, 19 -- charset="US-ASCII" 11, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
The reason the histology types want to avoid glutaraldehyde fixation is that it hardens tissue much more than formalin alone making it difficult to cut. Also, it causes "excessive" uptake of eosin in a routine H&E stain. My recommendation would be to fix by perfusion with buffered formalin, then remove a small portion and put in a form+glut fix for EM.
Geoff
oshel1pe-at-cmich.edu wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Stephane, } } Karnovsky's works fine for routine histology. It } does not have to be used in cacodylate buffer. } Phosphate works fine. Maunsbach and Afzelius, } "Biomedical Electron Microscopy"* has excellent } comparative micrographs of cacodylate and } phosphate buffers. } Your problem is less likely to be over-fixation } and more likely to be under-fixation. Fixing of } light-level histology tissue pieces really takes } 24 to 48 hours**. The formalin penetrates quickly } enough, it just doesn't fix that quickly. Glut } penetrates more slowly, but fixes quicker when it } gets in. } Wouldn't help to fix first in formalin then } change to Karnovsky's for your EM. The benefit of } using glut would be gone. } } Phil } } *Excellent text. Every bio EM person should have this book. } **Times for complete fixation in formalin is a } common thread on the histonet mailing list. } Usually grumping about how clinical turn-around } times are too fast for proper/complete fixation. } } } } Hi to all! } } I am struggling with a professor because we have } } 2 different opinions on the following problem: } } We have a project involving the analysis of rat } } organs both in histology and in EM. The } } histology part only requires H&E staining, no } } special labeling and no immunofluorescence. } } No immunolabeling for EM neither. } } Well the histologists want the whole organs to } } be fixed for histology, then I could get a small } } piece for EM. No problem for me. But they want } } the organs fixed in formalin and I want them } } fixed in Karnovsky (Formaldehyde + } } glutaraldehyde). I wonder why it would not be } } possible to fix the whole organs in } } Karnovsky.ÝIs Karnovsky fixation a problem for } } histology??! } } I know glutaraldehyde should not be used for IF, } } but we have no IF planned. I also know that } } glutaradehyde can lead to overfixation } } artifacts, but the material is being processed } } immediately and not intended to be stored for a } } long time. Another issue may be that } } histologists do not wish to work with cacodylate } } buffer, but they could simply change the } } fixation medium once I got my piece of tissue. } } I would appreciate your opinion and comments on } } the feasibility of using Karnovsky as fixation } } medium for histology. } } I wish you all have such a wonderful shiny day as we have here in Vienna. } } Best regards, } } } } Stephane } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
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I have to agree with Gary. You need to know WHY they want them moved. Is it a fire hazard, a fume issue, etc. You might point out to them that it is far more dangerous to have the pumps in the same room than to have them isolated. This involves inhaling the fumes, etc. If they persist, you MUST insist that they put in writing that they will pay to fix any problems (e.g., poor vacuum to the instrument) that result from this move. They must realize the consequences of this forced move.
JB
} } What is the "safety" issue? Is this Brownian activity } of a useless entity? Some units are good but others } are not. Are they really doing something to make } the situation better or are they just trying to } justify their existence? } } This safety stuff is really getting irritating } and divisive. } } gary g. } } } } Title-Subject: [Filtered] Maximum lenght of vacuum lines } } } } Question: All, } } } } The oil mechanical vacuum pumps (a few each of belt drive and direct } } drive) for our 4 ea EM's are currently located in a service spline } } directly behind the instruments. Currently, the pump to scope } } distance is about 4-5 feet using rubber hose. Our safety folks are } } asking that we relocate the pumps. This will involve 1) moving them } } into the scope lab itself or into another room at the end of the } } hallway. I'm worried about noise and vibration if we relocate into } } the lab. If we put all the pumps into a single room it will involve } } long vacuuum line runs. Safety insists we will use hard tubing. The } } vacuum line runs could be up to 20 or more feet between the pumps and } } EM's. Also, the tubing run will involved 2 right angle bends. How far } } can I locate the pumps from scopes? Does it matter if the bends are } } mitered. Should I use larger diameter tubing than the current rubber } } hose? } } } } Thanks, OWen } }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Has anyone tried converting an SEM in to something that could be used for e-beam lithography?
I have an old SEM sitting around and a researcher has his eye on it to do something like that. I don't know what to tell him or where to start looking for ideas.
Thanks
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to learn more. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu May 8 12:31:50 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48HVlxE013276 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 12:31:48 -0500 8, 20 -- Received: from [128.114.125.126] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 33915737 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 102012950; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu HELO [128.114.25.231]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 102012945 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:41 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230900c448e8d5b53c-at-[128.114.25.231]} 8, 20 -- Date: Thu, 8 May 2008 10:31:40 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: SEM into E-beam litho? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I'm in a new EM lab and they don't have any books on EM, go figure. What I'm wondering is: What is the best way to embed nerve (rat spinal cord and sciatic nerve). I don't have a lot of nerve experience, just a lot of nerve ;).
Also my student wants to know how long can something sit in Karnovsky's before something bad happens to the ultrastructure. I told her it's better to leave something stored in buffer but then we started wondering-how long is too long when it comes to leaving something in fix. They left their first samples in Karnovsky's for 6 months, is that pushing it?
Thanks in advance for your answers and advice.
Paula :-) VMRF Core EM/Confocal Facility VA Hospital San Diego 858-552-8585 x2397
____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ
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Valery Ray - neither affiliated with nor having any interest in the above vendors ========================= www.partbeamsystech.com
-----Original Message----- X-from: jmkrupp-at-ucsc.edu [mailto:jmkrupp-at-ucsc.edu] Sent: Thursday, May 08, 2008 2:17 PM To: vray-at-partbeamsystech.com
Hi:
Has anyone tried converting an SEM in to something that could be used for e-beam lithography?
I have an old SEM sitting around and a researcher has his eye on it to do something like that. I don't know what to tell him or where to start looking for ideas.
Thanks
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to learn more. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu May 8 12:31:50 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48HVlxE013276 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 12:31:48 -0500 8, 20 -- Received: from [128.114.125.126] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 33915737 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 102012950; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu HELO [128.114.25.231]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 102012945 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:41 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230900c448e8d5b53c-at-[128.114.25.231]} 8, 20 -- Date: Thu, 8 May 2008 10:31:40 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: SEM into E-beam litho? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 24, 29 -- From vray-at-partbeamsystech.com Thu May 8 14:21:37 2008 24, 29 -- Received: from n3.bullet.mail.re3.yahoo.com (n3.bullet.mail.re3.yahoo.com [68.142.237.110]) 24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m48JLYpZ031787 24, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 14:21:34 -0500 24, 29 -- Received: from [68.142.237.87] by n3.bullet.mail.re3.yahoo.com with NNFMP; 08 May 2008 11:09:06 -0000 24, 29 -- Received: from [69.147.75.181] by t3.bullet.re3.yahoo.com with NNFMP; 08 May 2008 19:16:32 -0000 24, 29 -- Received: from [127.0.0.1] by omp102.mail.re1.yahoo.com with NNFMP; 08 May 2008 19:16:32 -0000 24, 29 -- X-Yahoo-Newman-Id: 589713.1286.bm-at-omp102.mail.re1.yahoo.com 24, 29 -- Message-ID: {589713.1286.bm-at-omp102.mail.re1.yahoo.com} 24, 29 -- Received: (qmail 39806 invoked from network); 8 May 2008 19:16:32 -0000 24, 29 -- Received: from unknown (HELO cp1198275a) (partbeamsystech-at-75.67.54.109 with login) 24, 29 -- by smtp109.plus.mail.re1.yahoo.com with SMTP; 8 May 2008 19:16:32 -0000 24, 29 -- X-YMail-OSG: 8vjElyYVM1k2ieayGHogkQ0Yi_gyQI4zvawFqD6H.3QMxUDD5ar4sDRV7De0D7IYio8gEwIc19ib9ETT11JCw601bFiiH1Y4SCPQ2mYuWAHstwmBqYIPIrm4c9ncP2Gabog7NqBKyTgpdOr4kkvvA7ZHrRX8h8524EjKGmatKB5KjWmPII7d85xjokjq1RHhC8Fy5xIe18JTHprXzQ-- 24, 29 -- X-Yahoo-Newman-Property: ymail-3 24, 29 -- Reply-To: {vray-at-partbeamsystech.com} 24, 29 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 24, 29 -- To: {jmkrupp-at-ucsc.edu} 24, 29 -- Cc: {microscopy-at-microscopy.com} 24, 29 -- Subject: RE: [Microscopy] SEM into E-beam litho? 24, 29 -- Date: Thu, 8 May 2008 15:19:03 -0400 24, 29 -- Organization: PBST / MEO Engineering 24, 29 -- MIME-Version: 1.0 24, 29 -- Content-Type: text/plain; 24, 29 -- charset="us-ascii" 24, 29 -- Content-Transfer-Encoding: 7bit 24, 29 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 24, 29 -- Thread-Index: AcixN7ZO5krRWDStSBGWioRQ14XLvQACCJ8g 24, 29 -- In-Reply-To: {200805081816.m48IGwAX020151-at-ns.microscopy.com} 24, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I would like to know the justification for moving the pumps. Is it that the exhaust is not properly ventilated? Could it be that lab equipment is not allowed in utility spaces? Fire code?
} From mail-at-ns.microscopy.com Wed May 7 20:51:31 2008 } Date: Wed, 7 May 2008 19:49:36 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: opmills-at-mtu.edu } Subject: [Microscopy] viaWWW: Maximum lenght of vacuum lines } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] Maximum lenght of vacuum lines } } Question: All, } } The oil mechanical vacuum pumps (a few each of belt drive and direct } drive) for our 4 ea EM's are currently located in a service spline } directly behind the instruments. Currently, the pump to scope } distance is about 4-5 feet using rubber hose. Our safety folks are } asking that we relocate the pumps. This will involve 1) moving them } into the scope lab itself or into another room at the end of the } hallway. I'm worried about noise and vibration if we relocate into } the lab. If we put all the pumps into a single room it will involve } long vacuuum line runs. Safety insists we will use hard tubing. The } vacuum line runs could be up to 20 or more feet between the pumps and } EM's. Also, the tubing run will involved 2 right angle bends. How far } can I locate the pumps from scopes? Does it matter if the bends are } mitered. Should I use larger diameter tubing than the current rubber } hose? } } Thanks, OWen } } } Login Host: 141.219.192.45 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Wed May 7 19:21:57 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m480Ls5u007655 } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 7 May 2008 19:21:55 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c447f812bbc1-at-[206.69.208.22]} } 9, 11 -- Date: Wed, 7 May 2008 19:21:53 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Maximum lenght of vacuum lines } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu May 8 13:52:13 2008 9, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48Iq9nK025868 9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 13:52:12 -0500 9, 12 -- Received: (from jquinn-at-localhost) 9, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m48Hon504793; 9, 12 -- Thu, 8 May 2008 13:50:49 -0400 9, 12 -- Date: Thu, 8 May 2008 13:50:49 -0400 9, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 9, 12 -- Message-Id: {200805081750.m48Hon504793-at-www.matscieng.sunysb.edu} 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- Subject: re: Maximum lenght of vacuum lines ==============================End of - Headers==============================
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Email: ldemp-at-mse.ufl.edu Name: Amelia Dempere
Organization: University of Florida
Title-Subject: [Filtered] ON-LINE CERTIFICATE IN MATERIALS CHARACTERIZATION AT THE UNIVERSITY OF FLORIDA
Question: The Major Analytical Instrumentation Center (MAIC) in the Materials Science and Engineering Department at the University of Florida is offering, starting fall 2008, an on-line Certificate in Materials Characterization. Details of the 9-credit certificate, delivered through UF EDGE (Electronic Delivery of Graduate Engineering), can be found at http://www.ufedge.eng.ufl.edu/pdf/brochure/MSE_Cert_EDGE_7.pdf Please contact Dr. Pamela Dickrell, Director of UF-EDGE or Dr. Luisa Amelia Dempere, Director of MAIC, for questions or additional information.
Has anyone tried converting an SEM in to something that could be used for e-beam lithography?
I have an old SEM sitting around and a researcher has his eye on it to do something like that. I don't know what to tell him or where to start looking for ideas.
Thanks
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to learn more. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu May 8 18:20:07 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48HVlxE013276 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 12:31:48 -0500 8, 20 -- Received: from [128.114.125.126] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 33915737 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 102012950; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu HELO [128.114.25.231]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 102012945 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:41 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230900c448e8d5b53c-at-[128.114.25.231]} 8, 20 -- Date: Thu, 8 May 2008 10:31:40 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: SEM into E-beam litho? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We have problem with filament life on our Tecnai12, with diffusion pump and IGP. The scope is 5 years old. We are using W filaments supplied by FEI. We are getting between 20 to 30 hrs, rarely up to 50 hrs of life from a filament. Usually we operate at low emission settings, less than 10 microA. The filament current is stable and does not fluctuate more than 10% of the selected value. Scope performance has not changed. We have had several attempts by FEI engineers to find out the cause which they have failed to do so far. This problem is not operator related since we have experimented with saturating carefully the filament and then leaving the beam on at 120kV until the filament burned out without anyone using the scope during that period. The filament lasted 27 hrs.
This problem has started about a year and a half ago. Before that we had unbelievably long filament lifetimes. The record is 1097 hrs!!! Usually we used to get lifetimes consistently over 300 hrs, with typical time between 500 to 700 hrs. Since the problem developed we have replaced the IGP, the ceramic insulator in the gun, gun chamber O-rings, specimen stage air-lock valve, we have tested different Wehnelt caps, boxes of filaments old and new, the outputs of the power supply have been measured and they are stable and within the specs and nothing seems to make a difference. The Wehnelt cap aperture size and distance is consistently the same as at the time we were getting hundreds of hours of lifetime. Typically the scope is operated at vacuum in the range of 10 to 20 log readings which corresponds to 1x10-7 to 5x10-7 Torr. At the same vacuum levels we used to get very long lifetimes.
Any clues or suggestions what could have gone wrong.
Thank you,
Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel:951 827 2998 fax: 951 827 2489
bozhilov-at-ucr.edu ========================
==============================Original Headers============================== 11, 19 -- From bozhilov-at-ucr.edu Thu May 8 18:10:15 2008 11, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48NADaf028934 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 18:10:13 -0500 11, 19 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 11, 19 -- by sentoku.ucr.edu (MOS 3.8.5-GA) 11, 19 -- with ESMTP id CIK99288 11, 19 -- for {microscopy-at-microscopy.com} ; 11, 19 -- Thu, 8 May 2008 16:00:12 -0700 (PDT) 11, 19 -- Mime-Version: 1.0 (Apple Message framework v753) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {82E8CADD-D286-4002-AC0A-C7B026B269F3-at-ucr.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 11, 19 -- Subject: Tecnai12 filament life 11, 19 -- Date: Thu, 8 May 2008 15:58:42 -0700 11, 19 -- X-Mailer: Apple Mail (2.753) 11, 19 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentoku.ucr.edu) ==============================End of - Headers==============================
I'm in a new EM lab and they don't have any books on EM, go figure. What I'm wondering is: What is the best way to embed nerve (rat spinal cord and sciatic nerve). I don't have a lot of nerve experience, just a lot of nerve ;).
Also my student wants to know how long can something sit in Karnovsky's before something bad happens to the ultrastructure. I told her it's better to leave something stored in buffer but then we started wondering-how long is too long when it comes to leaving something in fix. They left their first samples in Karnovsky's for 6 months, is that pushing it?
Thanks in advance for your answers and advice.
Paula :-) VMRF Core EM/Confocal Facility VA Hospital San Diego 858-552-8585 x2397
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==============================Original Headers============================== 7, 20 -- From patpxs-at-yahoo.com Thu May 8 18:34:22 2008 7, 20 -- Received: from web50609.mail.re2.yahoo.com (web50609.mail.re2.yahoo.com [206.190.38.248]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m48HbifQ014152 7, 20 -- for {microscopy-at-msa.microscopy.com} ; Thu, 8 May 2008 12:37:44 -0500 7, 20 -- Received: (qmail 88810 invoked by uid 60001); 8 May 2008 17:31:03 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 20 -- b=jXtYY/aq82vAzZJwOGTIAovTYMBe9nVsI6icAaKfVmFPKSptMUDC3CrCOOMmAQxWtjeLh+1mbqO+g4wmXntoNfex2p/DPMsorG2ukjbRtQj/nkas1fwjKQXXRkNP0sB6PwzDqiJnOKlGjTVD6QUC/yTuUxIzmmmNy3ygibCQNQY=; 7, 20 -- X-YMail-OSG: 5mkEnQoVM1mvRG12NKUxonQSSDyNhGLBzDffPtYlsIo7VLAJipgvQEcimZeilCZcw6EZNHGujOhzrZlQDAeFYLJAkq8zV7T1EsJYyVatiDAU5c.qTvd9mi4Q1Ho- 7, 20 -- Received: from [132.239.85.200] by web50609.mail.re2.yahoo.com via HTTP; Thu, 08 May 2008 10:31:03 PDT 7, 20 -- X-Mailer: YahooMailWebService/0.7.185 7, 20 -- Date: Thu, 8 May 2008 10:31:03 -0700 (PDT) 7, 20 -- From: Paula Sicurello {patpxs-at-yahoo.com} 7, 20 -- Reply-To: patpxs-at-yahoo.com 7, 20 -- Subject: Embedding Nerve 7, 20 -- To: MSA BB {microscopy-at-msa.microscopy.com} 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Message-ID: {695636.88701.qm-at-web50609.mail.re2.yahoo.com} ==============================End of - Headers==============================
We have problem with filament life on our Tecnai12, with diffusion pump and IGP. The scope is 5 years old. We are using W filaments supplied by FEI. We are getting between 20 to 30 hrs, rarely up to 50 hrs of life from a filament. Usually we operate at low emission settings, less than 10 microA. The filament current is stable and does not fluctuate more than 10% of the selected value. Scope performance has not changed. We have had several attempts by FEI engineers to find out the cause which they have failed to do so far. This problem is not operator related since we have experimented with saturating carefully the filament and then leaving the beam on at 120kV until the filament burned out without anyone using the scope during that period. The filament lasted 27 hrs.
This problem has started about a year and a half ago. Before that we had unbelievably long filament lifetimes. The record is 1097 hrs!!! Usually we used to get lifetimes consistently over 300 hrs, with typical time between 500 to 700 hrs. Since the problem developed we have replaced the IGP, the ceramic insulator in the gun, gun chamber O-rings, specimen stage air-lock valve, we have tested different Wehnelt caps, boxes of filaments old and new, the outputs of the power supply have been measured and they are stable and within the specs and nothing seems to make a difference. The Wehnelt cap aperture size and distance is consistently the same as at the time we were getting hundreds of hours of lifetime. Typically the scope is operated at vacuum in the range of 10 to 20 log readings which corresponds to 1x10-7 to 5x10-7 Torr. At the same vacuum levels we used to get very long lifetimes.
Any clues or suggestions what could have gone wrong.
Thank you,
Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel:951 827 2998 fax: 951 827 2489
bozhilov-at-ucr.edu ========================
==============================Original Headers============================== 11, 19 -- From bozhilov-at-ucr.edu Thu May 8 18:41:07 2008 11, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48NADaf028934 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 18:10:13 -0500 11, 19 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 11, 19 -- by sentoku.ucr.edu (MOS 3.8.5-GA) 11, 19 -- with ESMTP id CIK99288 11, 19 -- for {microscopy-at-microscopy.com} ; 11, 19 -- Thu, 8 May 2008 16:00:12 -0700 (PDT) 11, 19 -- Mime-Version: 1.0 (Apple Message framework v753) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {82E8CADD-D286-4002-AC0A-C7B026B269F3-at-ucr.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 11, 19 -- Subject: Tecnai12 filament life 11, 19 -- Date: Thu, 8 May 2008 15:58:42 -0700 11, 19 -- X-Mailer: Apple Mail (2.753) 11, 19 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentoku.ucr.edu) ==============================End of - Headers==============================
Take a look at the most recent "Microscopy Today" where I talked about resolution and eucentric position.
If you wish to have the highest resolution you forget about the eucentric point and lower the sample further into the lens (requires a higher current to focus). The shorter focal length reduces aberrations, increases the magnification and increases the resolution capabilities of the instrument.
Good luck
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {s.miao-at-sheffield.ac.uk} To: {protrain-at-emcourses.com} Sent: Thursday, May 08, 2008 2:02 AM
Owen,
I have to agree with Gary. You need to know WHY they want them moved. Is it a fire hazard, a fume issue, etc. You might point out to them that it is far more dangerous to have the pumps in the same room than to have them isolated. This involves inhaling the fumes, etc. If they persist, you MUST insist that they put in writing that they will pay to fix any problems (e.g., poor vacuum to the instrument) that result from this move. They must realize the consequences of this forced move.
JB
} } What is the "safety" issue? Is this Brownian activity } of a useless entity? Some units are good but others } are not. Are they really doing something to make } the situation better or are they just trying to } justify their existence? } } This safety stuff is really getting irritating } and divisive. } } gary g. } } } } Title-Subject: [Filtered] Maximum lenght of vacuum lines } } } } Question: All, } } } } The oil mechanical vacuum pumps (a few each of belt drive and direct } } drive) for our 4 ea EM's are currently located in a service spline } } directly behind the instruments. Currently, the pump to scope } } distance is about 4-5 feet using rubber hose. Our safety folks are } } asking that we relocate the pumps. This will involve 1) moving them } } into the scope lab itself or into another room at the end of the } } hallway. I'm worried about noise and vibration if we relocate into } } the lab. If we put all the pumps into a single room it will involve } } long vacuuum line runs. Safety insists we will use hard tubing. The } } vacuum line runs could be up to 20 or more feet between the pumps and } } EM's. Also, the tubing run will involved 2 right angle bends. How far } } can I locate the pumps from scopes? Does it matter if the bends are } } mitered. Should I use larger diameter tubing than the current rubber } } hose? } } } } Thanks, OWen } }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I would like to know the justification for moving the pumps. Is it that the exhaust is not properly ventilated? Could it be that lab equipment is not allowed in utility spaces? Fire code?
} From mail-at-ns.microscopy.com Wed May 7 20:51:31 2008 } Date: Wed, 7 May 2008 19:49:36 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: opmills-at-mtu.edu } Subject: [Microscopy] viaWWW: Maximum lenght of vacuum lines } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] Maximum lenght of vacuum lines } } Question: All, } } The oil mechanical vacuum pumps (a few each of belt drive and direct } drive) for our 4 ea EM's are currently located in a service spline } directly behind the instruments. Currently, the pump to scope } distance is about 4-5 feet using rubber hose. Our safety folks are } asking that we relocate the pumps. This will involve 1) moving them } into the scope lab itself or into another room at the end of the } hallway. I'm worried about noise and vibration if we relocate into } the lab. If we put all the pumps into a single room it will involve } long vacuuum line runs. Safety insists we will use hard tubing. The } vacuum line runs could be up to 20 or more feet between the pumps and } EM's. Also, the tubing run will involved 2 right angle bends. How far } can I locate the pumps from scopes? Does it matter if the bends are } mitered. Should I use larger diameter tubing than the current rubber } hose? } } Thanks, OWen } } } Login Host: 141.219.192.45 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Wed May 7 19:21:57 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m480Ls5u007655 } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 7 May 2008 19:21:55 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c447f812bbc1-at-[206.69.208.22]} } 9, 11 -- Date: Wed, 7 May 2008 19:21:53 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Maximum lenght of vacuum lines } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 12 -- From jquinn-at-www.matscieng.sunysb.edu Thu May 8 19:00:17 2008 9, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 9, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48Iq9nK025868 9, 12 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 13:52:12 -0500 9, 12 -- Received: (from jquinn-at-localhost) 9, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m48Hon504793; 9, 12 -- Thu, 8 May 2008 13:50:49 -0400 9, 12 -- Date: Thu, 8 May 2008 13:50:49 -0400 9, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 9, 12 -- Message-Id: {200805081750.m48Hon504793-at-www.matscieng.sunysb.edu} 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- Subject: re: Maximum lenght of vacuum lines ==============================End of - Headers==============================
Valery Ray - neither affiliated with nor having any interest in the above vendors ========================= www.partbeamsystech.com
-----Original Message----- X-from: jmkrupp-at-ucsc.edu [mailto:jmkrupp-at-ucsc.edu] Sent: Thursday, May 08, 2008 2:17 PM To: vray-at-partbeamsystech.com
Hi:
Has anyone tried converting an SEM in to something that could be used for e-beam lithography?
I have an old SEM sitting around and a researcher has his eye on it to do something like that. I don't know what to tell him or where to start looking for ideas.
Thanks
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to learn more. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu May 8 12:31:50 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48HVlxE013276 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 12:31:48 -0500 8, 20 -- Received: from [128.114.125.126] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 33915737 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 102012950; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu HELO [128.114.25.231]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 102012945 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:41 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230900c448e8d5b53c-at-[128.114.25.231]} 8, 20 -- Date: Thu, 8 May 2008 10:31:40 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: SEM into E-beam litho? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 24, 29 -- From vray-at-partbeamsystech.com Thu May 8 19:05:24 2008 24, 29 -- Received: from n3.bullet.mail.re3.yahoo.com (n3.bullet.mail.re3.yahoo.com [68.142.237.110]) 24, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m48JLYpZ031787 24, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 14:21:34 -0500 24, 29 -- Received: from [68.142.237.87] by n3.bullet.mail.re3.yahoo.com with NNFMP; 08 May 2008 11:09:06 -0000 24, 29 -- Received: from [69.147.75.181] by t3.bullet.re3.yahoo.com with NNFMP; 08 May 2008 19:16:32 -0000 24, 29 -- Received: from [127.0.0.1] by omp102.mail.re1.yahoo.com with NNFMP; 08 May 2008 19:16:32 -0000 24, 29 -- X-Yahoo-Newman-Id: 589713.1286.bm-at-omp102.mail.re1.yahoo.com 24, 29 -- Message-ID: {589713.1286.bm-at-omp102.mail.re1.yahoo.com} 24, 29 -- Received: (qmail 39806 invoked from network); 8 May 2008 19:16:32 -0000 24, 29 -- Received: from unknown (HELO cp1198275a) (partbeamsystech-at-75.67.54.109 with login) 24, 29 -- by smtp109.plus.mail.re1.yahoo.com with SMTP; 8 May 2008 19:16:32 -0000 24, 29 -- X-YMail-OSG: 8vjElyYVM1k2ieayGHogkQ0Yi_gyQI4zvawFqD6H.3QMxUDD5ar4sDRV7De0D7IYio8gEwIc19ib9ETT11JCw601bFiiH1Y4SCPQ2mYuWAHstwmBqYIPIrm4c9ncP2Gabog7NqBKyTgpdOr4kkvvA7ZHrRX8h8524EjKGmatKB5KjWmPII7d85xjokjq1RHhC8Fy5xIe18JTHprXzQ-- 24, 29 -- X-Yahoo-Newman-Property: ymail-3 24, 29 -- Reply-To: {vray-at-partbeamsystech.com} 24, 29 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 24, 29 -- To: {jmkrupp-at-ucsc.edu} 24, 29 -- Cc: {microscopy-at-microscopy.com} 24, 29 -- Subject: RE: [Microscopy] SEM into E-beam litho? 24, 29 -- Date: Thu, 8 May 2008 15:19:03 -0400 24, 29 -- Organization: PBST / MEO Engineering 24, 29 -- MIME-Version: 1.0 24, 29 -- Content-Type: text/plain; 24, 29 -- charset="us-ascii" 24, 29 -- Content-Transfer-Encoding: 7bit 24, 29 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 24, 29 -- Thread-Index: AcixN7ZO5krRWDStSBGWioRQ14XLvQACCJ8g 24, 29 -- In-Reply-To: {200805081816.m48IGwAX020151-at-ns.microscopy.com} 24, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Hi Formalin or formaldehyde. I normally stay away from formalin for TEM work due to the MeOH. I have never liked the results. Am I alone in this? David
On May 8, 2008, at 9:26 AM, mcauliff-at-umdnj.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The reason the histology types want to avoid glutaraldehyde fixation } is } that it hardens tissue much more than formalin alone making it } difficult } to cut. } Also, it causes "excessive" uptake of eosin in a routine H&E stain. } My recommendation would be to fix by perfusion with buffered formalin, } then remove a small portion and put in a form+glut fix for EM. } } Geoff } } } oshel1pe-at-cmich.edu wrote: } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Stephane, } } } } Karnovsky's works fine for routine histology. It } } does not have to be used in cacodylate buffer. } } Phosphate works fine. Maunsbach and Afzelius, } } "Biomedical Electron Microscopy"* has excellent } } comparative micrographs of cacodylate and } } phosphate buffers. } } Your problem is less likely to be over-fixation } } and more likely to be under-fixation. Fixing of } } light-level histology tissue pieces really takes } } 24 to 48 hours**. The formalin penetrates quickly } } enough, it just doesn't fix that quickly. Glut } } penetrates more slowly, but fixes quicker when it } } gets in. } } Wouldn't help to fix first in formalin then } } change to Karnovsky's for your EM. The benefit of } } using glut would be gone. } } } } Phil } } } } *Excellent text. Every bio EM person should have this book. } } **Times for complete fixation in formalin is a } } common thread on the histonet mailing list. } } Usually grumping about how clinical turn-around } } times are too fast for proper/complete fixation. } } } } } } } Hi to all! } } } I am struggling with a professor because we have } } } 2 different opinions on the following problem: } } } We have a project involving the analysis of rat } } } organs both in histology and in EM. The } } } histology part only requires H&E staining, no } } } special labeling and no immunofluorescence. } } } No immunolabeling for EM neither. } } } Well the histologists want the whole organs to } } } be fixed for histology, then I could get a small } } } piece for EM. No problem for me. But they want } } } the organs fixed in formalin and I want them } } } fixed in Karnovsky (Formaldehyde + } } } glutaraldehyde). I wonder why it would not be } } } possible to fix the whole organs in } } } Karnovsky.ÝIs Karnovsky fixation a problem for } } } histology??! } } } I know glutaraldehyde should not be used for IF, } } } but we have no IF planned. I also know that } } } glutaradehyde can lead to overfixation } } } artifacts, but the material is being processed } } } immediately and not intended to be stored for a } } } long time. Another issue may be that } } } histologists do not wish to work with cacodylate } } } buffer, but they could simply change the } } } fixation medium once I got my piece of tissue. } } } I would appreciate your opinion and comments on } } } the feasibility of using Karnovsky as fixation } } } medium for histology. } } } I wish you all have such a wonderful shiny day as we have here in } } } Vienna. } } } Best regards, } } } } } } Stephane } } } } } } -- } -- } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583 } mcauliff-at-umdnj.edu } ********************************************** } } } } } } ==============================Original } Headers============================== } 10, 29 -- From mcauliff-at-umdnj.edu Thu May 8 09:09:16 2008 } 10, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU } [130.219.34.124]) } 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m48E9BGX018870 } 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 09:09:14 } -0500 } 10, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) } 10, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id } 11E48A7B8C } 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 } 10:09:11 -0400 (EDT) } 10, 29 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) } 10, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 2739DA7BB8 } 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 } 10:09:10 -0400 (EDT) } 10, 29 -- Received: from ([10.32.15.171]) } 10, 29 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.150219744; } 10, 29 -- Thu, 08 May 2008 10:08:45 -0400 } 10, 29 -- Received: from [127.0.0.1] ([10.32.15.102]) } 10, 29 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging } Server 6.3-5.02 (built } 10, 29 -- Oct 12 2007; 32bit)) with ESMTP id {0K0J003QJZALIZI0-at-umduwc02.umdnj.edu } } for } 10, 29 -- microscopy-at-microscopy.com; Thu, 08 May 2008 10:08:45 } -0400 (EDT) } 10, 29 -- Date: Thu, 08 May 2008 10:09:23 -0400 } 10, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} } 10, 29 -- Subject: Re: [Microscopy] Re: karnovsky and histology } 10, 29 -- In-reply-to: {200805081343.m48DhSsH015349-at-ns.microscopy.com} } 10, 29 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com } 10, 29 -- Message-id: {48230993.6040507-at-umdnj.edu} } 10, 29 -- MIME-version: 1.0 } 10, 29 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed } 10, 29 -- References: {200805081343.m48DhSsH015349-at-ns.microscopy.com} } 10, 29 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) } 10, 29 -- Content-Transfer-Encoding: 8bit } 10, 29 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by } ns.microscopy.com id m48E9BGX018870 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 23 -- From Elliott-at-arizona.edu Thu May 8 19:09:27 2008 5, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4909RBr007578 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 19:09:27 -0500 5, 23 -- Received: from gandalfs_amavis (amavis8.email.arizona.edu [10.0.0.236]) 5, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2516A64ADF5 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 15:30:03 -0700 (MST) 5, 23 -- Received: from [150.135.145.124] (unknown [150.135.145.124]) 5, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 6759F64CA1B 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 15:29:59 -0700 (MST) 5, 23 -- Message-Id: {7854C660-A518-45DE-B296-2F789D9FB66C-at-arizona.edu} 5, 23 -- From: David Elliott {Elliott-at-arizona.edu} 5, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 23 -- In-Reply-To: {200805081626.m48GQR8q004687-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 5, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) 5, 23 -- Subject: Re: [Microscopy] karnovsky and histology 5, 23 -- Date: Thu, 8 May 2008 15:29:57 -0700 5, 23 -- References: {200805081626.m48GQR8q004687-at-ns.microscopy.com} 5, 23 -- X-Mailer: Apple Mail (2.919.2) 5, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4909RBr007578 ==============================End of - Headers==============================
This can be done to write to wafers or reticles. Wafer writing is hampered by an inability to blank the beam and precisely position it for the next write, and so on. The other issue is writing speed. Writing to a wafer can take a very long time. Plus, the SEM beam must be very stable during the entire write time and across the entire wafer or the area being written.
Commercial units are from MEBES and include several models. For converting a SEM, Raith makes adapters to do this:
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==============================Original Headers============================== 12, 20 -- From gary-at-gaugler.com Thu May 8 19:16:19 2008 12, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m490GJBR023353 12, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 19:16:19 -0500 12, 20 -- Message-Id: {200805090016.m490GJBR023353-at-ns.microscopy.com} 12, 20 -- Received: (qmail 26490 invoked from network); 8 May 2008 14:29:39 -0700 12, 20 -- Received: by simscan 1.1.0 ppid: 26487, pid: 26488, t: 0.1178s 12, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 12, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 12, 20 -- by smtp1 with SMTP; 8 May 2008 14:29:39 -0700 12, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 20 -- Date: Thu, 08 May 2008 14:29:38 -0700 12, 20 -- To: jmkrupp-at-ucsc.edu 12, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 12, 20 -- Subject: Re: [Microscopy] SEM into E-beam litho? 12, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 12, 20 -- In-Reply-To: {200805081838.m48Ic3Kp023664-at-ns.microscopy.com} 12, 20 -- References: {200805081838.m48Ic3Kp023664-at-ns.microscopy.com} 12, 20 -- Mime-Version: 1.0 12, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-739E5990 ==============================End of - Headers==============================
Hi All, I have been using a UV resin along with HPMA to clear and adhere filters with algae (GN6 mixed ester nitrocellulose) to slides. It has worked well and did not interfere with the autofluorescence of chlorophyll or phycoerythrin. Electro-Lite has discontinued that particular resin and I need to find a similar replacement. Does anyone have any suggestions about other UV manufacturers or experience with autofluorescence and UV resins? Thanks much, Ann. -- Ann St. Amand, Ph.D., CLP President PhycoTech, Inc. 620 Broad St., Suite 100 St. Joseph, Michigan 49085 USA
Krassimir, If the electronics are stable (especially the filament drive ... I don't consider 10% anything close to stable, 1-2%/hr is satisfactory), then I would be looking for a vacuum leak in the immediate gun area. You haven't said exactly where you're measuring the vacuum. That can make a big difference, depending upon the vacuum design (I'm not familiar with the Tecnai scopes). One can very easily get pressure differences of a decade or more between sections of the vacuum system even without a significant leak. Have you put a leak detector or RGA on it?
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] Sent: Thursday, May 08, 2008 7:44 PM To: kenconverse-at-qualityimages.biz
We have problem with filament life on our Tecnai12, with diffusion pump and IGP. The scope is 5 years old. We are using W filaments supplied by FEI. We are getting between 20 to 30 hrs, rarely up to 50 hrs of life from a filament. Usually we operate at low emission settings, less than 10 microA. The filament current is stable and does not fluctuate more than 10% of the selected value. Scope performance has not changed. We have had several attempts by FEI engineers to find out the cause which they have failed to do so far. This problem is not operator related since we have experimented with saturating carefully the filament and then leaving the beam on at 120kV until the filament burned out without anyone using the scope during that period. The filament lasted 27 hrs.
This problem has started about a year and a half ago. Before that we had unbelievably long filament lifetimes. The record is 1097 hrs!!! Usually we used to get lifetimes consistently over 300 hrs, with typical time between 500 to 700 hrs. Since the problem developed we have replaced the IGP, the ceramic insulator in the gun, gun chamber O-rings, specimen stage air-lock valve, we have tested different Wehnelt caps, boxes of filaments old and new, the outputs of the power supply have been measured and they are stable and within the specs and nothing seems to make a difference. The Wehnelt cap aperture size and distance is consistently the same as at the time we were getting hundreds of hours of lifetime. Typically the scope is operated at vacuum in the range of 10 to 20 log readings which corresponds to 1x10-7 to 5x10-7 Torr. At the same vacuum levels we used to get very long lifetimes.
Any clues or suggestions what could have gone wrong.
Thank you,
Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel:951 827 2998 fax: 951 827 2489
bozhilov-at-ucr.edu ========================
==============================Original Headers============================== 11, 19 -- From bozhilov-at-ucr.edu Thu May 8 18:41:07 2008 11, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48NADaf028934 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 18:10:13 -0500 11, 19 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 11, 19 -- by sentoku.ucr.edu (MOS 3.8.5-GA) 11, 19 -- with ESMTP id CIK99288 11, 19 -- for {microscopy-at-microscopy.com} ; 11, 19 -- Thu, 8 May 2008 16:00:12 -0700 (PDT) 11, 19 -- Mime-Version: 1.0 (Apple Message framework v753) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {82E8CADD-D286-4002-AC0A-C7B026B269F3-at-ucr.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 11, 19 -- Subject: Tecnai12 filament life 11, 19 -- Date: Thu, 8 May 2008 15:58:42 -0700 11, 19 -- X-Mailer: Apple Mail (2.753) 11, 19 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentoku.ucr.edu) ==============================End of - Headers==============================
==============================Original Headers============================== 23, 25 -- From kenconverse-at-qualityimages.biz Thu May 8 20:49:10 2008 23, 25 -- Received: from mta9.adelphia.net (mta9.adelphia.net [68.168.78.199]) 23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m491n92p014349 23, 25 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 20:49:10 -0500 23, 25 -- Received: from Ken ([72.227.100.25]) by mta9.adelphia.net 23, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 23, 25 -- id {20080509015131.DRWM26458.mta9.adelphia.net-at-Ken} ; 23, 25 -- Thu, 8 May 2008 21:51:31 -0400 23, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 23, 25 -- To: {bozhilov-at-ucr.edu} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 23, 25 -- Subject: RE: [Microscopy] Tecnai12 filament life 23, 25 -- Date: Thu, 8 May 2008 21:48:59 -0400 23, 25 -- Message-ID: {000001c8b176$d9e54e00$6401a8c0-at-Ken} 23, 25 -- MIME-Version: 1.0 23, 25 -- Content-Type: text/plain; 23, 25 -- charset="us-ascii" 23, 25 -- X-Priority: 3 (Normal) 23, 25 -- X-MSMail-Priority: Normal 23, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 23, 25 -- Importance: Normal 23, 25 -- Thread-Index: AcixZWS9bTBja2R6RGStmjX3JEbNLwAD6dog 23, 25 -- In-Reply-To: {200805082343.m48Nhxdf025683-at-ns.microscopy.com} 23, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 23, 25 -- Content-Transfer-Encoding: 8bit 23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m491n92p014349 ==============================End of - Headers==============================
Jon, The SEM "is" your basic e-beam lithography instrument. All you need is external control (see Joe Nabity at http://www.jcnabity.com/ ) and an electrostatic beam blanker (see Earl Weltmer at http://www.semservice.com ) Stage automation can be a plus, but for small scale experiments it isn't necessary.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: jmkrupp-at-ucsc.edu [mailto:jmkrupp-at-ucsc.edu] Sent: Thursday, May 08, 2008 3:20 PM To: kenconverse-at-qualityimages.biz
Hi:
Has anyone tried converting an SEM in to something that could be used for e-beam lithography?
I have an old SEM sitting around and a researcher has his eye on it to do something like that. I don't know what to tell him or where to start looking for ideas.
Thanks
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to learn more. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Thu May 8 12:31:50 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48HVlxE013276 8, 20 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 12:31:48 -0500 8, 20 -- Received: from [128.114.125.126] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 33915737 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- Received: by email-prod-fe-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 102012950; Thu, 08 May 2008 10:31:43 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu HELO [128.114.25.231]) 8, 20 -- by email-prod-fe-2.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 102012945 for microscopy-at-microscopy.com; Thu, 08 May 2008 10:31:41 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230900c448e8d5b53c-at-[128.114.25.231]} 8, 20 -- Date: Thu, 8 May 2008 10:31:40 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: SEM into E-beam litho? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 20, 25 -- From kenconverse-at-qualityimages.biz Thu May 8 20:57:34 2008 20, 25 -- Received: from mta13.adelphia.net (mta13.adelphia.net [68.168.78.44]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m491vYGP030806 20, 25 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 20:57:34 -0500 20, 25 -- Received: from Ken ([72.227.100.25]) by mta13.adelphia.net 20, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 20, 25 -- id {20080509015641.TBDK21875.mta13.adelphia.net-at-Ken} ; 20, 25 -- Thu, 8 May 2008 21:56:41 -0400 20, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 25 -- To: {jmkrupp-at-ucsc.edu} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 20, 25 -- Subject: RE: [Microscopy] SEM into E-beam litho? 20, 25 -- Date: Thu, 8 May 2008 21:57:24 -0400 20, 25 -- Message-ID: {000101c8b178$06bb28e0$6401a8c0-at-Ken} 20, 25 -- MIME-Version: 1.0 20, 25 -- Content-Type: text/plain; 20, 25 -- charset="us-ascii" 20, 25 -- X-Priority: 3 (Normal) 20, 25 -- X-MSMail-Priority: Normal 20, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 20, 25 -- Importance: Normal 20, 25 -- Thread-Index: AcixRWxshHDJ74DmQiKgu9ezkcmBXwAMYqcw 20, 25 -- In-Reply-To: {200805081920.m48JKH6D031576-at-ns.microscopy.com} 20, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 20, 25 -- Content-Transfer-Encoding: 8bit 20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m491vYGP030806 ==============================End of - Headers==============================
Hi Krassimir! I know I won't offer a very deep thought, but sometimes there are very simple answers to complex problems (however the contrary is also possible ;-)). Perhaps you are using a bad serie of filaments. Did you try with another serie? Can you analyse the filaments with a SEM? Regards, Stephane
----- Original Message ---- X-from: "bozhilov-at-ucr.edu" {bozhilov-at-ucr.edu} To: nizets2-at-yahoo.com Sent: Friday, May 9, 2008 1:45:32 AM
We have problem with filament life on our Tecnai12, with diffusion pump and IGP. The scope is 5 years old. We are using W filaments supplied by FEI. We are getting between 20 to 30 hrs, rarely up to 50 hrs of life from a filament. Usually we operate at low emission settings, less than 10 microA. The filament current is stable and does not fluctuate more than 10% of the selected value. Scope performance has not changed. We have had several attempts by FEI engineers to find out the cause which they have failed to do so far. This problem is not operator related since we have experimented with saturating carefully the filament and then leaving the beam on at 120kV until the filament burned out without anyone using the scope during that period. The filament lasted 27 hrs.
This problem has started about a year and a half ago. Before that we had unbelievably long filament lifetimes. The record is 1097 hrs!!! Usually we used to get lifetimes consistently over 300 hrs, with typical time between 500 to 700 hrs. Since the problem developed we have replaced the IGP, the ceramic insulator in the gun, gun chamber O-rings, specimen stage air-lock valve, we have tested different Wehnelt caps, boxes of filaments old and new, the outputs of the power supply have been measured and they are stable and within the specs and nothing seems to make a difference. The Wehnelt cap aperture size and distance is consistently the same as at the time we were getting hundreds of hours of lifetime. Typically the scope is operated at vacuum in the range of 10 to 20 log readings which corresponds to 1x10-7 to 5x10-7 Torr. At the same vacuum levels we used to get very long lifetimes.
Any clues or suggestions what could have gone wrong.
Thank you,
Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel:951 827 2998 fax: 951 827 2489
bozhilov-at-ucr.edu ========================
==============================Original Headers============================== 11, 19 -- From bozhilov-at-ucr.edu Thu May 8 18:41:07 2008 11, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m48NADaf028934 11, 19 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 18:10:13 -0500 11, 19 -- Received: from [138.23.185.162] (igppb116g.ucr.edu [138.23.185.162]) 11, 19 -- by sentoku.ucr.edu (MOS 3.8.5-GA) 11, 19 -- with ESMTP id CIK99288 11, 19 -- for {microscopy-at-microscopy.com} ; 11, 19 -- Thu, 8 May 2008 16:00:12 -0700 (PDT) 11, 19 -- Mime-Version: 1.0 (Apple Message framework v753) 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- Message-Id: {82E8CADD-D286-4002-AC0A-C7B026B269F3-at-ucr.edu} 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- To: microscopy-at-microscopy.com 11, 19 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 11, 19 -- Subject: Tecnai12 filament life 11, 19 -- Date: Thu, 8 May 2008 15:58:42 -0700 11, 19 -- X-Mailer: Apple Mail (2.753) 11, 19 -- X-Junkmail-Whitelist: YES (by domain whitelist at sentoku.ucr.edu) ==============================End of - Headers==============================
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==============================Original Headers============================== 23, 22 -- From nizets2-at-yahoo.com Fri May 9 03:25:54 2008 23, 22 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m498Psq2021522 23, 22 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 03:25:54 -0500 23, 22 -- Received: (qmail 11283 invoked by uid 60001); 9 May 2008 08:25:54 -0000 23, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 22 -- s=s1024; d=yahoo.com; 23, 22 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 23, 22 -- b=WKGYM0CgTOA8vZRHqMMtCx+t2XQMMS7LEH/P+pREXKn7mzoiD5kyejSoUUJN9RHG21vkV1WpiSUUBKb0RBHXIoKu+du2VLTkxMS49vOO5AFG7/a9jRtZwnA3KHy18ev3zvaua9AQciXU+3nHT0KCAmr2M3QTWs4i9l5zXiqGj68=; 23, 22 -- X-YMail-OSG: LrWJ1mYVM1lfFscYGoABWAWFIgRQ7dtE8Ulci5EtlebH7ttymkwEQvekLzj2LudA.pvWXjMZGd4uHxM1ivyJiMxov9XQEEbdvMD5wOESK_GmeK53ILv4rUik2X5auhXIJ2B.Rl73d7WfhilBTh32FpMGgqUVacobdOiUj6Sc0mffsqg55BY7 23, 22 -- Received: from [80.122.101.100] by web37403.mail.mud.yahoo.com via HTTP; Fri, 09 May 2008 01:25:54 PDT 23, 22 -- X-Mailer: YahooMailRC/975.23 YahooMailWebService/0.7.185 23, 22 -- Date: Fri, 9 May 2008 01:25:54 -0700 (PDT) 23, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 23, 22 -- Subject: Re: [Microscopy] Tecnai12 filament life 23, 22 -- To: bozhilov-at-ucr.edu 23, 22 -- Cc: microscopy-at-microscopy.com 23, 22 -- MIME-Version: 1.0 23, 22 -- Content-Type: text/plain; charset=iso-8859-1 23, 22 -- Message-ID: {58881.10720.qm-at-web37403.mail.mud.yahoo.com} 23, 22 -- Content-Transfer-Encoding: 8bit 23, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m498Psq2021522 ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society is holding an Optical Techniques Workshop on Wednesday, June 11, 2008, at the College of Microscopy in Westmont, IL. Our topic is optical and microspectroscopic analysis of cross-sectioned materials. Registration information and a preliminary program can be found on our website:
www.midwestmicroscopy.org
Please follow the link to the Meetings page.
Regards,
Elaine Schumacher M3S Program Coordinator
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
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==============================Original Headers============================== 10, 23 -- From eschumacher-at-mccrone.com Fri May 9 07:49:42 2008 10, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m49CnggR009224 10, 23 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 07:49:42 -0500 10, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Fri, 9 May 2008 07:49:42 -0500 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="iso-8859-1" 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Subject: Meeting of the Midwest Microscopy and Microanalysis Society: June 11 Optical Techniques Workshop 10, 23 -- Date: Fri, 9 May 2008 07:49:41 -0500 10, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DE3C-at-MCCRONEMSG.tmg.mccrone.com} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Meeting of the Midwest Microscopy and Microanalysis Society: June 11 Optical Techniques Workshop 10, 23 -- Thread-Index: Acix0yYCvo+iGnuwT0mEsXtgcEwfDA== 10, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 09 May 2008 12:49:42.0516 (UTC) FILETIME=[26AD3340:01C8B1D3] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m49CnggR009224 ==============================End of - Headers==============================
I know this seems too obvious, but why can't you just slice the fresh organs, take a slice for EM, then fix the rest of the organ. This procedure is okay with the American FDA for pharmaceutical tissue collection, why is this a problem for your situation?
I am aware that this method is easier for some tissues than others. For brain and spinal cord, perfusion would be the way to go. Reproductive organs also might create some trouble, but the other major organs should be fine.
I'm curious to know how many animals and how many organs you are considering. You might not get ideal fixation, but there IS a compromise that you and the histology group should be able to reach.
Regards, Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, May 08, 2008 10:06 AM To: Sobocinski, Gregg
Hi to all! I am struggling with a professor because we have 2 different opinions on the following problem: We have a project involving the analysis of rat organs both in histology and in EM. The histology part only requires H&E staining, no special labeling and no immunofluorescence. No immunolabeling for EM neither. Well the histologists want the whole organs to be fixed for histology, then I could get a small piece for EM. No problem for me. But they want the organs fixed in formalin and I want them fixed in Karnovsky (Formaldehyde + glutaraldehyde). I wonder why it would not be possible to fix the whole organs in Karnovsky. Is Karnovsky fixation a problem for histology??! I know glutaraldehyde should not be used for IF, but we have no IF planned. I also know that glutaradehyde can lead to overfixation artifacts, but the material is being processed immediately and not intended to be stored for a long time. Another issue may be that histologists do not wish to work with cacodylate buffer, but they could simply change the fixation medium once I got my piece of tissue. I would appreciate your opinion and comments on the feasibility of using Karnovsky as fixation medium for histology. I wish you all have such a wonderful shiny day as we have here in Vienna. Best regards,
Stephane
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==============================Original Headers============================== 5, 21 -- From nizets2-at-yahoo.com Thu May 8 03:13:39 2008 5, 21 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m488DZjd010555 5, 21 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 03:13:36 -0500 5, 21 -- Received: (qmail 60241 invoked by uid 60001); 8 May 2008 08:06:54 -0000 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 21 -- s=s1024; d=yahoo.com; 5, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 21 -- b=0a+ib7bGzn5UyLRM/aZxWE5q+QT38jnNV+rltJ3QXlB+d0bWHoATYCJWxU3Dbt8VeZDKsvMY4OX4nnmnbM1HbBADOm43gevaMlv4Nzo9JcTQEi/SPgABRTHzyDsZ6RfUSisLMbyESBvC0u/YX4t6bWWIv+n3m/E8QvcliGmgxU0=; 5, 21 -- X-YMail-OSG: e0OmUT0VM1mHQCDlFJwDqPHQv5no8a79H0g7x_MhbuZ2XESOrSD_Yo7EkEyneDWdKYoiHAsq.B5QPk0IkGkpUxNj_2k5V8p.fEfa5snAUAZAAfyQC_UnBEcE8EU- 5, 21 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Thu, 08 May 2008 01:06:54 PDT 5, 21 -- X-Mailer: YahooMailRC/975.23 YahooMailWebService/0.7.185 5, 21 -- Date: Thu, 8 May 2008 01:06:54 -0700 (PDT) 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 21 -- Subject: karnovsky and histology 5, 21 -- To: microscopy-at-microscopy.com 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 5, 21 -- Message-ID: {643729.60203.qm-at-web37406.mail.mud.yahoo.com} 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m488DZjd010555 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 25 -- From greggps-at-umich.edu Fri May 9 09:37:45 2008 15, 25 -- Received: from OWA02.adsroot.itcs.umich.edu (owa02.adsroot.itcs.umich.edu [141.211.27.139]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m49EbjV7028400 15, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 09:37:45 -0500 15, 25 -- Received: from ECLUST2-VS3.adsroot.itcs.umich.edu ([141.211.27.143]) by OWA02.adsroot.itcs.umich.edu with Microsoft SMTPSVC(6.0.3790.1830); 15, 25 -- Fri, 9 May 2008 10:37:45 -0400 15, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 15, 25 -- Content-class: urn:content-classes:message 15, 25 -- MIME-Version: 1.0 15, 25 -- Content-Type: text/plain; 15, 25 -- charset="iso-8859-1" 15, 25 -- Subject: RE: [Microscopy] Karnovsky and histology 15, 25 -- Date: Fri, 9 May 2008 10:37:39 -0400 15, 25 -- Message-ID: {3F08051AB847224F9B89DAB301137EF402A46B52-at-ECLUST2-VS3.adsroot.itcs.umich.edu} 15, 25 -- In-Reply-To: {200805081405.m48E5ejT018452-at-ns.microscopy.com} 15, 25 -- X-MS-Has-Attach: 15, 25 -- X-MS-TNEF-Correlator: 15, 25 -- Thread-Topic: [Microscopy] Karnovsky and histology 15, 25 -- Thread-Index: AcixFKYsyy65B6GsTNu2IVjSplAgcwAzDDGg 15, 25 -- References: {200805081405.m48E5ejT018452-at-ns.microscopy.com} 15, 25 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 15, 25 -- To: {nizets2-at-yahoo.com} , {microscopy-at-microscopy.com} 15, 25 -- X-OriginalArrivalTime: 09 May 2008 14:37:45.0315 (UTC) FILETIME=[3EB9D330:01C8B1E2] 15, 25 -- Content-Transfer-Encoding: 8bit 15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m49EbjV7028400 ==============================End of - Headers==============================
Hi Listers For years I have been using Aclar film to grow cells on. I then fix, embed for TEM and polymerize my Epon. I then peel off the aclar and have my cells in the epon. Rather standard stuff. Recently I got some new film. It was thicker than the old (about which I do not care), but the film is now sticking to the Epon. It is VERY hard to remove. This is a problem for me. Has anyone else experienced this? Are there any suggestions on what to do about this? Thank you David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 4, 20 -- From Elliott-at-Arizona.edu Fri May 9 13:05:52 2008 4, 20 -- Received: from smtpgate.email.arizona.edu (frodo.email.Arizona.EDU [128.196.133.168]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m49I5qKd015989 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 May 2008 13:05:52 -0500 4, 20 -- Received: from frodos_amavis (amavis10.email.arizona.edu [10.0.0.238]) 4, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id C1007458C04 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 May 2008 11:05:51 -0700 (MST) 4, 20 -- Received: from [150.135.145.124] (unknown [150.135.145.124]) 4, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 96FB6458B9F 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 9 May 2008 11:05:51 -0700 (MST) 4, 20 -- Message-Id: {0DFFE286-D682-47AB-842C-11C529C37CF7-at-Arizona.edu} 4, 20 -- From: David Elliott {Elliott-at-Arizona.edu} 4, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 4, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- Mime-Version: 1.0 (Apple Message framework v919.2) 4, 20 -- Subject: aclar film question 4, 20 -- Date: Fri, 9 May 2008 11:05:51 -0700 4, 20 -- X-Mailer: Apple Mail (2.919.2) 4, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
My histologist friend tells me that tissues fixed in glut are more difficult to cut for LM (if embedded in paraffin). I believe they are harder and more brittle. Also, the staining properties may shift away from what the pathologist is used to seeing. So, most histotechs adhere to a standarized set of protocols for the sake of uniformity.
If the tissues are to be handled expeditiously, why not have them hand you formaldehyde fixed material (not Formalin, that contains methanol). You could prepare some good quality formaldehyde of the proper concentration for them and when they hand you your piece of tissue, slice it into smaller pieces and fix it in Karnovski.
JB
} } We have a project involving the analysis of rat } organs both in histology and in EM. The } histology part only requires H&E staining, no } special labeling and no immunofluorescence. } No immunolabeling for EM neither. } Well the histologists want the whole organs to } be fixed for histology, then I could get a small } piece for EM. No problem for me. But they want } the organs fixed in formalin and I want them } fixed in Karnovsky (Formaldehyde + } glutaraldehyde). I wonder why it would not be } possible to fix the whole organs in } Karnovsky.ÝIs Karnovsky fixation a problem for } histology??! } I know glutaraldehyde should not be used for IF, } but we have no IF planned. I also know that } glutaradehyde can lead to overfixation } artifacts, but the material is being processed } immediately and not intended to be stored for a } long time. Another issue may be that } histologists do not wish to work with cacodylate } buffer, but they could simply change the } fixation medium once I got my piece of tissue.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
What is a good method of attaching a silicon chip to an aluminum SEM stub, so that: a) the electrical contact is good, allowing high-resolution imaging (50 kx or higher); and b) the mechanical stability is good, so that the specimen can survive shipping?
I am aware of the following materials: colloidal carbon in isopropyl alcohol conductive carbon adhesive tabs and I am curious about the experience of other microscopists.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 4, 24 -- From donc-at-asmicro.com Fri May 9 16:40:21 2008 4, 24 -- Received: from smtp106.sbc.mail.re2.yahoo.com (smtp106.sbc.mail.re2.yahoo.com [68.142.229.99]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m49LeLtp014758 4, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 16:40:21 -0500 4, 24 -- Received: (qmail 67498 invoked from network); 9 May 2008 21:40:20 -0000 4, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-76.252.40.5 with login) 4, 24 -- by smtp106.sbc.mail.re2.yahoo.com with SMTP; 9 May 2008 21:40:20 -0000 4, 24 -- X-YMail-OSG: o2ScAX0VM1neKwVGyKkYSnGG9KLdBGJ8.0BOmBx91NhekozvPv_ouy37Jadm5I9nF6G3Q1yP5VYRL94G5gcIvS6v5LIywl.rnMT.2FyexelNcEmPqmna_t39WNbVaz6zC_f3JsE2eIVvrtSkv9PgpXkH 4, 24 -- X-Yahoo-Newman-Property: ymail-3 4, 24 -- Message-ID: {007801c8b21d$1f066e50$9b01a8c0-at-asm15} 4, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 4, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 4, 24 -- Subject: Sample mounting 4, 24 -- Date: Fri, 9 May 2008 17:39:09 -0400 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- format=flowed; 4, 24 -- charset="iso-8859-1"; 4, 24 -- reply-type=original 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Normal way is to use Carbon double sticky tabs. If the die is SOI, then coat the outer edges with colloidal Ag. This will prevent it from charging. For shipping, single stub storage tubes work well.
Another mounting method is to use colloidal Ag to mount the die. Care is needed to avoid wicking over the edges of the die.
gary g.
At 02:42 PM 5/9/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Fri May 9 16:54:40 2008 7, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m49LseqU027617 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 16:54:40 -0500 7, 20 -- Message-Id: {200805092154.m49LseqU027617-at-ns.microscopy.com} 7, 20 -- Received: (qmail 25470 invoked from network); 9 May 2008 14:54:40 -0700 7, 20 -- Received: by simscan 1.1.0 ppid: 25466, pid: 25467, t: 0.1093s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by smtp2 with SMTP; 9 May 2008 14:54:39 -0700 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Fri, 09 May 2008 14:54:37 -0700 7, 20 -- To: donc-at-asmicro.com 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] Sample mounting 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200805092142.m49LgFLU017289-at-ns.microscopy.com} 7, 20 -- References: {200805092142.m49LgFLU017289-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-55B409C ==============================End of - Headers==============================
Dear Don, We have had problems in the past with high-resolution imaging (} 100,000X) of samples secured with double-sided carbon adhesive discs, although I use them all the time for routine, low-mag work. At high mags the sample crept under the beam. I use cyanoacrylate ("super glue") to hold the sample securely, then a bit of colloidal carbon in one corner to ground them. Unfortunately, you cannot then recover them. I have also used a hot-glue gun to secure samples, again grounding with colloidal graphite. Soaking in acetone later will remove the colloidal carbon and loosen the glue, so the sample can be removed. Regards,
-----Original Message----- X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com] Sent: May 9, 2008 2:47 PM To: maryflet-at-interchange.ubc.ca
What is a good method of attaching a silicon chip to an aluminum SEM stub, so that: a) the electrical contact is good, allowing high-resolution imaging (50 kx or higher); and b) the mechanical stability is good, so that the specimen can survive shipping?
I am aware of the following materials: colloidal carbon in isopropyl alcohol conductive carbon adhesive tabs and I am curious about the experience of other microscopists.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 4, 24 -- From donc-at-asmicro.com Fri May 9 16:40:21 2008 4, 24 -- Received: from smtp106.sbc.mail.re2.yahoo.com (smtp106.sbc.mail.re2.yahoo.com [68.142.229.99]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m49LeLtp014758 4, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 16:40:21 -0500 4, 24 -- Received: (qmail 67498 invoked from network); 9 May 2008 21:40:20 -0000 4, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-76.252.40.5 with login) 4, 24 -- by smtp106.sbc.mail.re2.yahoo.com with SMTP; 9 May 2008 21:40:20 -0000 4, 24 -- X-YMail-OSG: o2ScAX0VM1neKwVGyKkYSnGG9KLdBGJ8.0BOmBx91NhekozvPv_ouy37Jadm5I9nF6G3Q1yP5VYR L94G5gcIvS6v5LIywl.rnMT.2FyexelNcEmPqmna_t39WNbVaz6zC_f3JsE2eIVvrtSkv9PgpXkH 4, 24 -- X-Yahoo-Newman-Property: ymail-3 4, 24 -- Message-ID: {007801c8b21d$1f066e50$9b01a8c0-at-asm15} 4, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 4, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 4, 24 -- Subject: Sample mounting 4, 24 -- Date: Fri, 9 May 2008 17:39:09 -0400 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- format=flowed; 4, 24 -- charset="iso-8859-1"; 4, 24 -- reply-type=original 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 35 -- From maryflet-at-interchange.ubc.ca Fri May 9 17:28:07 2008 13, 35 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) 13, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m49MS63r008637 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 17:28:06 -0500 13, 35 -- X-Ubc-Received: from mr5.mail-relay.ubc.ca (localhost [127.0.0.1]) 13, 35 -- by localhost (Postfix) with SMTP id 7E975114D4 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 15:28:06 -0700 (PDT) 13, 35 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 13, 35 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 15:28:05 -0700 (PDT) 13, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 13, 35 -- by smtp.interchange.ubc.ca 13, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 13, 35 -- with ESMTP id {0K0M008XMH2S0U-at-smtp.interchange.ubc.ca} for 13, 35 -- microscopy-at-microscopy.com; Fri, 09 May 2008 15:28:05 -0700 (PDT) 13, 35 -- Date: Fri, 09 May 2008 15:27:44 -0700 13, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 13, 35 -- Subject: RE: [Microscopy] Sample mounting 13, 35 -- In-reply-to: {200805092146.m49Lkbm4024604-at-ns.microscopy.com} 13, 35 -- To: donc-at-asmicro.com 13, 35 -- Cc: microscopy-at-microscopy.com 13, 35 -- Reply-to: maryflet-at-interchange.ubc.ca 13, 35 -- Message-id: {0K0M008XNH2S0U-at-smtp.interchange.ubc.ca} 13, 35 -- Organization: Materials Eng. 13, 35 -- MIME-version: 1.0 13, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 13, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 13, 35 -- Content-type: text/plain; charset=us-ascii 13, 35 -- Content-transfer-encoding: 7bit 13, 35 -- Thread-index: AciyHikyqRzwHizZR226d8trt/8J7gABMzsg 13, 35 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.9.151242 13, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 13, 35 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, ECARD_KNOWN_DOMAINS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 13, 35 -- X-Spam-Level: 13, 35 -- X-Spam-Flag: No ==============================End of - Headers==============================
We have a FEI Quanta FEG SEM and would like to optimize it for low keV imaging. I really don't care if my microscope can perform above 2keV. The normal alignment procedure is to start at 30keV and align the top part of the column and then go to 1keV and align the lower part of the column. I am thinking that if we were to align the top part at 2keV and align the bottom part of the column at 1keV that we would be more optimized for 2keV and below. Does anyone think that this is a good idea or are there better ways to get optimized low keV images.
Richard
-- ........................................................................ Richard E. Stallcup II, PhD Applications Manager, NanoWorks® Tools; Senior Scientist
The information contained in this transmission is privileged and confidential and is intended only for the use of the addressee(s).
This e-mail is sent for business reasons only and should be considered confidential. ........................................................................
==============================Original Headers============================== 10, 25 -- From rstallcup-at-zyvex.com Fri May 9 18:13:21 2008 10, 25 -- Received: from lsv-dfw-mproxy01.zyvex.com (host50.zyvex.com [216.138.97.50]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m49NDLsP022039 10, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 May 2008 18:13:21 -0500 10, 25 -- Received: from [10.0.0.152] ([10.0.0.152]) 10, 25 -- (authenticated bits=0) 10, 25 -- by lsv-dfw-mproxy01.zyvex.com (8.13.1/8.13.1) with ESMTP id m49NDKTL027261 10, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 9 May 2008 18:13:20 -0500 10, 25 -- Message-ID: {4824DA66.1030302-at-zyvex.com} 10, 25 -- Date: Fri, 09 May 2008 18:12:38 -0500 10, 25 -- From: Richard Stallcup {rstallcup-at-zyvex.com} 10, 25 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) 10, 25 -- MIME-Version: 1.0 10, 25 -- To: Microscopy-at-microscopy.com 10, 25 -- Subject: Low keV imaging 10, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-Spam-Status: No, score=-1.4 required=5.0 tests=ALL_TRUSTED 10, 25 -- autolearn=disabled version=3.1.8 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on 10, 25 -- lsv-dfw-mproxy01.zyvex.com 10, 25 -- X-Virus-Scanned: ClamAV 0.90.3/7030/Sun May 4 23:02:10 2008 on lsv-dfw-mproxy01.zyvex.com 10, 25 -- X-Virus-Status: Clean 10, 25 -- Received-SPF: pass (lsv-dfw-mproxy01.zyvex.com: 10.0.0.152 is authenticated by a trusted mechanism) ==============================End of - Headers==============================
I have been involved with several instances of moving rough pumps, and being a former student of Will Bigelow, dutifully calculated the conductance using the class notes that are now in his text book on vacuum technology. OK, the conductance and pumping speeds match, but what will surprise you is that the rough pump line suddenly has a volume that has increased by quite a lot, and when you hit that button that says "Pump", the "WHOOOMPF" you will hear the first time that roughing valve opens will really be a shock. Consider carefully whether your system can withstand the internal turbulence that this will cause. EDX vendors believe that pumping turbulence throws particulate material around with sufficient force to put pinholes in EDX detector's thin windows. There is also potential for physically moving thin apertures, lifting pole pieces, and who knows what else. The best thing to do here (aside from keeping pumping lines short) is install a throttle valve where the roughing line connects to the microscope. One way to do this is insert a butterfly valve in the large diameter roughing line in which you have drilled a small hole, maybe on the order of .25-.5 inch. Close the butterfly valve before pumping, then open it after the initial surge has subsided.
John Mardinly Numonyx
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Thursday, May 08, 2008 5:00 PM To: MARDINLY, A
Owen,
I have to agree with Gary. You need to know WHY they want them moved. Is it a fire hazard, a fume issue, etc. You might point out to them that it is far more dangerous to have the pumps in the same room than to have them isolated. This involves inhaling the fumes, etc. If they persist, you MUST insist that they put in writing that they will pay to fix any problems (e.g., poor vacuum to the instrument) that result from this move. They must realize the consequences of this forced move.
JB
} } What is the "safety" issue? Is this Brownian activity } of a useless entity? Some units are good but others } are not. Are they really doing something to make } the situation better or are they just trying to } justify their existence? } } This safety stuff is really getting irritating } and divisive. } } gary g. } } } } Title-Subject: [Filtered] Maximum lenght of vacuum lines } } } } Question: All, } } } } The oil mechanical vacuum pumps (a few each of belt drive and direct } } drive) for our 4 ea EM's are currently located in a service spline } } directly behind the instruments. Currently, the pump to scope } } distance is about 4-5 feet using rubber hose. Our safety folks are } } asking that we relocate the pumps. This will involve 1) moving them } } into the scope lab itself or into another room at the end of the } } hallway. I'm worried about noise and vibration if we relocate into } } the lab. If we put all the pumps into a single room it will involve } } long vacuuum line runs. Safety insists we will use hard tubing. The } } vacuum line runs could be up to 20 or more feet between the pumps and } } EM's. Also, the tubing run will involved 2 right angle bends. How far } } can I locate the pumps from scopes? Does it matter if the bends are } } mitered. Should I use larger diameter tubing than the current rubber } } hose? } } } } Thanks, OWen } }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I will second Mary's problems with double-sided carbon tabs. I have the same trouble with double-sided carbon tape. I use colloidal carbon paint to mount my samples for high mag work, making sure the paint is fully dry before I put the sample in the SEM. I've also used Mary's SuperGlue suggestion. Use minimal glue and ground with carbon paint. CrystalBond thermal adhesive works well and has the added ability to be removed with acetone.
maryflet-at-interchange.ubc.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Don, } We have had problems in the past with high-resolution imaging (} 100,000X) of } samples secured with double-sided carbon adhesive discs, although I use them } all the time for routine, low-mag work. At high mags the sample crept under } the beam. I use cyanoacrylate ("super glue") to hold the sample securely, } then a bit of colloidal carbon in one corner to ground them. Unfortunately, } you cannot then recover them. I have also used a hot-glue gun to secure } samples, again grounding with colloidal graphite. Soaking in acetone later } will remove the colloidal carbon and loosen the glue, so the sample can be } removed. } Regards, } } Mary Fletcher } Electron Microscopist } Materials Eng. UBC } #309 - 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } Canada } Tel: 604-822-5648 } Fax: 604-822-3619 } email: maryflet-at-interchange.ubc.ca } } } -----Original Message----- } X-from: donc-at-asmicro.com [mailto:donc-at-asmicro.com] } Sent: May 9, 2008 2:47 PM } To: maryflet-at-interchange.ubc.ca } Subject: [Microscopy] Sample mounting } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } What is a good method of attaching a silicon chip to an aluminum SEM stub, } so that: } a) the electrical contact is good, allowing high-resolution imaging (50 kx } or higher); and } b) the mechanical stability is good, so that the specimen can survive } shipping? } } I am aware of the following materials: } colloidal carbon in isopropyl alcohol } conductive carbon adhesive tabs } and I am curious about the experience of other microscopists. } } regards, } Don } ============================================= } Don Chernoff, Ph.D., President } Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com } 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 } INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) } web: http://www.asmicro.com Fax: 317-895-5652 } [business activities: analytical services in AFM, AFM probes, consulting, } training, } calibration and test specimens, calibration and measurement software, } used NanoScope equipment.] } ============================================= } } } ==============================Original Headers============================== } 4, 24 -- From donc-at-asmicro.com Fri May 9 16:40:21 2008 } 4, 24 -- Received: from smtp106.sbc.mail.re2.yahoo.com } (smtp106.sbc.mail.re2.yahoo.com [68.142.229.99]) } 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } m49LeLtp014758 } 4, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 16:40:21 } -0500 } 4, 24 -- Received: (qmail 67498 invoked from network); 9 May 2008 21:40:20 } -0000 } 4, 24 -- Received: from unknown (HELO asm15) } (asmicro-at-sbcglobal.net-at-76.252.40.5 with login) } 4, 24 -- by smtp106.sbc.mail.re2.yahoo.com with SMTP; 9 May 2008 21:40:20 } -0000 } 4, 24 -- X-YMail-OSG: } o2ScAX0VM1neKwVGyKkYSnGG9KLdBGJ8.0BOmBx91NhekozvPv_ouy37Jadm5I9nF6G3Q1yP5VYR } L94G5gcIvS6v5LIywl.rnMT.2FyexelNcEmPqmna_t39WNbVaz6zC_f3JsE2eIVvrtSkv9PgpXkH } 4, 24 -- X-Yahoo-Newman-Property: ymail-3 } 4, 24 -- Message-ID: {007801c8b21d$1f066e50$9b01a8c0-at-asm15} } 4, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} } 4, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} } 4, 24 -- Subject: Sample mounting } 4, 24 -- Date: Fri, 9 May 2008 17:39:09 -0400 } 4, 24 -- MIME-Version: 1.0 } 4, 24 -- Content-Type: text/plain; } 4, 24 -- format=flowed; } 4, 24 -- charset="iso-8859-1"; } 4, 24 -- reply-type=original } 4, 24 -- Content-Transfer-Encoding: 7bit } 4, 24 -- X-Priority: 3 } 4, 24 -- X-MSMail-Priority: Normal } 4, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 } 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 13, 35 -- From maryflet-at-interchange.ubc.ca Fri May 9 17:28:07 2008 } 13, 35 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) } 13, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m49MS63r008637 } 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 17:28:06 -0500 } 13, 35 -- X-Ubc-Received: from mr5.mail-relay.ubc.ca (localhost [127.0.0.1]) } 13, 35 -- by localhost (Postfix) with SMTP id 7E975114D4 } 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 15:28:06 -0700 (PDT) } 13, 35 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) } 13, 35 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP } 13, 35 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 15:28:05 -0700 (PDT) } 13, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) } 13, 35 -- by smtp.interchange.ubc.ca } 13, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) } 13, 35 -- with ESMTP id {0K0M008XMH2S0U-at-smtp.interchange.ubc.ca} for } 13, 35 -- microscopy-at-microscopy.com; Fri, 09 May 2008 15:28:05 -0700 (PDT) } 13, 35 -- Date: Fri, 09 May 2008 15:27:44 -0700 } 13, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} } 13, 35 -- Subject: RE: [Microscopy] Sample mounting } 13, 35 -- In-reply-to: {200805092146.m49Lkbm4024604-at-ns.microscopy.com} } 13, 35 -- To: donc-at-asmicro.com } 13, 35 -- Cc: microscopy-at-microscopy.com } 13, 35 -- Reply-to: maryflet-at-interchange.ubc.ca } 13, 35 -- Message-id: {0K0M008XNH2S0U-at-smtp.interchange.ubc.ca} } 13, 35 -- Organization: Materials Eng. } 13, 35 -- MIME-version: 1.0 } 13, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 13, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 } 13, 35 -- Content-type: text/plain; charset=us-ascii } 13, 35 -- Content-transfer-encoding: 7bit } 13, 35 -- Thread-index: AciyHikyqRzwHizZR226d8trt/8J7gABMzsg } 13, 35 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.9.151242 } 13, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca } 13, 35 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, ECARD_KNOWN_DOMAINS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 } 13, 35 -- X-Spam-Level: } 13, 35 -- X-Spam-Flag: No } ==============================End of - Headers============================== } }
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The "WHOOOMPF" sound and much longer pumping time which you are describing will indeed take place if entire roughing line is vented and if roughing pump is stopped during the vent. One way to deal with it will be to install a throttle valve, as you described, but in this case one still has to deal with increased pumping time due to the need to pump air from the volume of roughing line during each circle.
Another way of running instrument with long roughing lines is to install into the roughing line electric (or pneumatic) shutoff valve as close as practical to the place where roughing line connect to SEM, and to control this valve from the relay which intended to stop roughing pump during vent and start it during pumping, in such a way that the valve is closed during vent and opened during pumping. Roughing pump should be connected directly to power and run all the time, even when instrument is vented, thus keeping roughing lines constantly evacuated, so this volume does not need to be pumped each circle. The operation, as far as SEM is concerned, will be the same as if roughing pump, with pumping speed limited by the conductance of the long roughing line, was installed where the shutoff valve now is.
This will save pumping time with, but have downside of increased hours on the roughing pump (bill pump rebuilding costs to Safety Department :)))).
Cheers,
Valery Ray ======================== www.partbeamsystech.com
-----Original Message----- X-from: A.MARDINLY-at-numonyx.com [mailto:A.MARDINLY-at-numonyx.com] Sent: Friday, May 09, 2008 7:53 PM To: vray-at-partbeamsystech.com
I have been involved with several instances of moving rough pumps, and being a former student of Will Bigelow, dutifully calculated the conductance using the class notes that are now in his text book on vacuum technology. OK, the conductance and pumping speeds match, but what will surprise you is that the rough pump line suddenly has a volume that has increased by quite a lot, and when you hit that button that says "Pump", the "WHOOOMPF" you will hear the first time that roughing valve opens will really be a shock. Consider carefully whether your system can withstand the internal turbulence that this will cause. EDX vendors believe that pumping turbulence throws particulate material around with sufficient force to put pinholes in EDX detector's thin windows. There is also potential for physically moving thin apertures, lifting pole pieces, and who knows what else. The best thing to do here (aside from keeping pumping lines short) is install a throttle valve where the roughing line connects to the microscope. One way to do this is insert a butterfly valve in the large diameter roughing line in which you have drilled a small hole, maybe on the order of .25-.5 inch. Close the butterfly valve before pumping, then open it after the initial surge has subsided.
John Mardinly Numonyx
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Thursday, May 08, 2008 5:00 PM To: MARDINLY, A
Owen,
I have to agree with Gary. You need to know WHY they want them moved. Is it a fire hazard, a fume issue, etc. You might point out to them that it is far more dangerous to have the pumps in the same room than to have them isolated. This involves inhaling the fumes, etc. If they persist, you MUST insist that they put in writing that they will pay to fix any problems (e.g., poor vacuum to the instrument) that result from this move. They must realize the consequences of this forced move.
JB
} } What is the "safety" issue? Is this Brownian activity } of a useless entity? Some units are good but others } are not. Are they really doing something to make } the situation better or are they just trying to } justify their existence? } } This safety stuff is really getting irritating } and divisive. } } gary g. } } } } Title-Subject: [Filtered] Maximum lenght of vacuum lines } } } } Question: All, } } } } The oil mechanical vacuum pumps (a few each of belt drive and direct } } drive) for our 4 ea EM's are currently located in a service spline } } directly behind the instruments. Currently, the pump to scope } } distance is about 4-5 feet using rubber hose. Our safety folks are } } asking that we relocate the pumps. This will involve 1) moving them } } into the scope lab itself or into another room at the end of the } } hallway. I'm worried about noise and vibration if we relocate into } } the lab. If we put all the pumps into a single room it will involve } } long vacuuum line runs. Safety insists we will use hard tubing. The } } vacuum line runs could be up to 20 or more feet between the pumps and } } EM's. Also, the tubing run will involved 2 right angle bends. How far } } can I locate the pumps from scopes? Does it matter if the bends are } } mitered. Should I use larger diameter tubing than the current rubber } } hose? } } } } Thanks, OWen } }
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
take a good look at the glass/porcelain base of the burnt filaments that lasted less than 30 hrs. Can you positively see deposited (tungsten) material on the base surface around the filament?
additionally - do you have at least an impression, if not positive observation, that emission current somewhat increases on its own at any time during filament life? Irrelevant to arcing. Does not have to go up steadily all the time from installation to burn-out. But at least a few hours of slow steady increase, however small.
If any of the above is a 'yes', then I might have an idea.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {bozhilov-at-ucr.edu} To: {vitalylazar-at-att.net} Sent: Thursday, May 08, 2008 7:42 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both m_jarnik-at-fccc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: m_jarnik-at-fccc.edu Name: Michal Jarnik
Organization: FCCC
Title-Subject: [Filtered] Durst Enblarger
Question: Due to renovations and limited storage space, we have to donate our Durst photgraphic enlarger, Durst Laborator S-45. A large, freestanding unit, good condition. Transport is your responsibility - and we need to move it soon. We also have a paper processor (Agfa, I believe) and some auxilliary equipment.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rstallcup-at-zyvex.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rstallcup-at-zyvex.com Name: Richard Stallcup
Organization: Zyvex Instruments
Title-Subject: [Filtered] Low keV imaging
Question: We have a FEI Quanta FEG SEM and would like to optimize it for low keV imaging. I really don't care if my microscope can perform above 2keV. The normal alignment procedure is to start at 30keV and align the top part of the column and then go to 1keV and align the lower part of the column.
I am thinking that if we were to align the top part at 2keV and align the bottom part of the column at 1keV that we would be more optimized for 2keV and below.
Does anyone think that this is a good idea or are there better ways to get optimized low keV images.
Can any of you advise me on the following needs/problems? We've done EM of glycerol-extracted insect light muscle (IFM) since 1965, but almost no fresh muscle and no LM histology whatsoever. Now at last I want a little good-quality wide-area LM of my particular material, quite large for an insect.
I've recently Karnovsky-fixed flight muscle in situ of a freshly dissected live giant waterbug (Lethocerus indicus), each dorsal longitudinal muscle of length 15 mm, and cross-sectional area 6 x 10 mm. I will take some surface fibers for my usual EM study. I am seeking a recommended fee-for-service lab that can embed three blocks in GMA to cut and stain LM sections 1-3 microns thick presenting three different views of the muscle.----- XS, LS tangent to surface in mid-muscle, and LS including insertion into cuticle at one end of the muscle so I can see the epidermal tendon cells that replace collagen tendons in arthropods. I'd like the largest practical block faces (how large is that in GMA?) for the first 2 views, but need only small-area block-face in the cuticle area
(The last may be a problem, bevel-trimming the block so cuticle is absent from the first few sections, then appear in thinnest possible profile in next few sections before it becomes too thick to let intact and undistorted sections be cut.) -- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
all right, then I suspect the deposit creates an electrical conductance pass between wehnelt and filament. If you look at the schematic diagram of HT generator, you will see this pass being electrically parallel to gun bias resistors. These resistors are switched back and forth for gun bias control in automatic bias circuit, that is common in modern EM guns. The less resistance - the higher the bias setting- the more current forced from the filament. This deposit may effectively increase the gun bias, with obvious consequences for filament life expectancy.
You may prevent this deposit from connecting the circuit, in the following way. Find a piece of copper/brass/bronze thin sheet or thick foil, some 10 mil to 20 mil will do. Actually can be any metal that will not corrode and is easy to solder. Cut a washer from this material. This washer must be soldered on top of filament holding nut, just 2 or 3 points soldering, no durability required. The external diameter of this washer must be little less than the external diameter of the filament holding nut, and the internal diameter must be much smaller, just enough to accommodate filament pins. Make sure of the following:
a) distance between filament pins and inner diameter of washer must be not less than 2.5mm, (3mm is good) - do not make it too large, otherwise it will not work; b) washer must not touch filament base - suppose it will bend and come very close to the base, that is OK, but must not touch the base; c) washer does not have to be perfectly even and round, yet (!!!) all must be flat and smooth, with no bumps and sharp points - solder, edges of the washer, etc.
Deposit will still form. But the washer will 'cast the shadow' on the outer part of filament base, so the deposit will not close the circuit.
The actual reasons for excessive tungsten evaporation/erosion may vary. Perhaps you have bad batch of filaments, which is less likely. Or perhaps your HT generator circuit is outside the specs which is more likely. Additionally, if my guess was correct, then a filament base with very rough surface may prolong filament life. Bottom line, this method worked for me, on Philips CM series TEMs and it is much preferred to any other remedy as long as it works. Actual configuration of this additional element may vary depending on wehnelt design for a particular TEM model.
I would e-mail you a picture of this fix but I am out of the country for another 2 weeks - the picture will be available then.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: "K. N. Bozhilov" {bozhilov-at-ucr.edu} To: "Vitaly Feingold" {vitalylazar-at-att.net} Sent: Saturday, May 10, 2008 9:18 AM
P.S.
A possible alternative - again presuming my guess was correct- a Kimball Physics LaB6 filament might be a solution. It has carbon heater and carbon does not evaporate much at these temperatures. In fact I installed this filament on a CM-10 with same problem a couple of months ago and it works so far without the modification that I described in the previous posting. Too shorter time to be certain whether it is a fix or not.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: "K. N. Bozhilov" {bozhilov-at-ucr.edu} To: "Vitaly Feingold" {vitalylazar-at-att.net} Sent: Saturday, May 10, 2008 9:18 AM
Don,
I too have experienced stability issues with carbon tape. In my line of work the Z axis is also critical and carbon tape contracts for many hours after the SEM is evacuated. I have had very good results with putting the silicon chip directly on an aluminum sample holder and using single sided SEM compatible copper tape with nickel colloid adhesive to tape it down. I use my tweezers to burnish the tape onto the chip top, edges, and aluminum holder. The conductive adhesive on the edges of the chip gives a good electrical connection. One nice feature of this technique is that you do not have any solvents that have to dry before imaging. The negative side of this technique is that some part of the chips top will have tape covering it and adhesive residue is left if you peel off the tape. Acetone will remove the residue.
I have successfully shipped samples all over the world that have been prepared this way.
Richard
........................................................................ Richard E. Stallcup II, PhD Applications Manager, NanoWorks® Tools; Senior Scientist
Greetings, This is a call for those attending the Microscopy and Microanalysis Meeting in Albuquerque, Aug.3-7 that would like to help with meeting activities by serving as a student bursary or volunteer. The activities are, but are not necessarily limited to, providing support in the short courses or symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth, monitoring use of the Internet Cafe, and assisting at social events or evening tutorials. We need your help to make the meetings run smoothly.
Students have the opportunity to apply for a Student Bursary which can defray meeting costs. Bursaries will be paid $10 an hour and may work up to a total of 40 hours during the course of the meetings. Checks for time worked will be given at the end of the meeting or whenever the bursary departs. Not only does being a bursary help with the cost of the meeting, it provides an opportunity where young scientists can meet and interact with the established microscopy community.
Volunteers (those other than students) with a desire to help with meeting activities would be appreciated too. Although not paid per hour as students, they will receive some compensation toward their meeting costs.
In addition, students as well as volunteers will get a $10 cash meal allotment for each morning and/or afternoon session worked and a Polo shirt.
The contact person this year that will coordinate the initial student bursary and volunteer sign-up is: Amanda Lawrence (alawrence-at-entomology.msstate.edu ). Clayton Loehn (cloehn-at-vt.edu) will be helping with coordination of activities once on site.
If you would like to help with meeting activities please contact me and I will add your name to the bursary/volunteer list. Students also will need to check the "I wish to apply for a bursary" on the registration form and need to be a members of MSA or MAS.
Shortly I will email a copy of the master task list to the volunteers and bursaries to allow them the opportunity sign-up for the areas and times they would like to work. This would then be sent back to me and list sign-up will be on a first-come first-served basis. Specific instructions for activities will be emailed closer to meeting time.
Thank you in advance for help and should you have questions concerning the process, please contact:
Amanda Lawrence (alawrence-at-entomology.msstate.edu) Electron Microscope Center 100 Twelve Lane Mississippi State University Mississippi State, MS 39762 (662)-325-3019 Work (662)-325-0246 Fax
==============================Original Headers============================== 13, 20 -- From ALawrence-at-entomology.msstate.edu Sun May 11 06:49:06 2008 13, 20 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4BBn6iJ031702 13, 20 -- for {microscopy-at-microscopy.com} ; Sun, 11 May 2008 06:49:06 -0500 13, 20 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 13, 20 -- with Novell_GroupWise; Sun, 11 May 2008 06:49:05 -0500 13, 20 -- Message-Id: {482696DC020000E60003D83B-at-mailhost2.groupwise.msstate.edu} 13, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 13, 20 -- Date: Sun, 11 May 2008 06:49:00 -0500 13, 20 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 13, 20 -- To: {microscopy-at-microscopy.com} 13, 20 -- Subject: M&M 2008 request for volunteers and bursaries 13, 20 -- References: {478F515B020000E6000314B8-at-mailhost2.groupwise.msstate.edu} 13, 20 -- {4825221B020000E60003D755-at-mailhost2.groupwise.msstate.edu} 13, 20 -- {482696DC020000E60003D83B-at-mailhost2.groupwise.msstate.edu} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=US-ASCII 13, 20 -- Content-Disposition: inline 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4BBn6iJ031702 ==============================End of - Headers==============================
Yes you are on the right lines for most instruments, but I do not know the FEI FEG too well.
The lower the kV that you use to align the instrument the better it should be at the lower levels. This is standard practice for most W SEM but in that case we often raise the anode to keep the gun fields optimised for best performance.
Good luck
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {rstallcup-at-zyvex.com} To: {protrain-at-emcourses.com} Sent: Saturday, May 10, 2008 12:14 AM
Hi Stephane,
I always used glutaraldehyde as secondary fixative and storage in cacodylate buffer. Tissue was initially fixed with OsO4 dissolved in Freon though as I worked with lung tissue (Freon/OsO4 prevented lung fluids dissolving in fixative or being washed away during intra-tracheal fixation). I only embedded tissue in Spurr's or LR White resin (think the latter), never paraffin wax, so fixed tissue 'hardness' wasn't an issue. However the tissue was only used for light microscopy and autoradiography production (inhaled U-235, Pu-239/238 oxides as hard insoluble particles). H&E stains were fine if a little dark tinted due to OsO4, and we could cut sections down to nominally 0.5 um for high quality light microscope images. Some occasional TEM samples were taken from the same tissue, although the hard actinide oxide particles played havoc with the diamond knife.
I only mention this as tissue sections prepared this way were in my opinion clearly superior to standard formalin/wax embedding of tissue (that’s not good for lung morphometry anyway). The main downside was the histology lab occasionally lost the sections when removing the hard resin from the sections on slides, as they batched them through on masse. For the autoradiographs we removed the resin ourselves in our labs (from CR-39 plastic) and took more individual care, always used fresh sodium ethoxide solutions (histology changed there's every week or so), and had few problems.
So it can be done, although everything needs to go up a notch from standard batched histology production in terms of time and cost.
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: Elliott-at-arizona.edu [mailto:Elliott-at-arizona.edu] Sent: 09 May 2008 01:15 To: kjmorris-at-well.ox.ac.uk
Hi Formalin or formaldehyde. I normally stay away from formalin for TEM work due to the MeOH. I have never liked the results. Am I alone in this? David
On May 8, 2008, at 9:26 AM, mcauliff-at-umdnj.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The reason the histology types want to avoid glutaraldehyde fixation } is } that it hardens tissue much more than formalin alone making it } difficult } to cut. } Also, it causes "excessive" uptake of eosin in a routine H&E stain. } My recommendation would be to fix by perfusion with buffered formalin, } then remove a small portion and put in a form+glut fix for EM. } } Geoff } } } oshel1pe-at-cmich.edu wrote: } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Stephane, } } } } Karnovsky's works fine for routine histology. It } } does not have to be used in cacodylate buffer. } } Phosphate works fine. Maunsbach and Afzelius, } } "Biomedical Electron Microscopy"* has excellent } } comparative micrographs of cacodylate and } } phosphate buffers. } } Your problem is less likely to be over-fixation } } and more likely to be under-fixation. Fixing of } } light-level histology tissue pieces really takes } } 24 to 48 hours**. The formalin penetrates quickly } } enough, it just doesn't fix that quickly. Glut } } penetrates more slowly, but fixes quicker when it } } gets in. } } Wouldn't help to fix first in formalin then } } change to Karnovsky's for your EM. The benefit of } } using glut would be gone. } } } } Phil } } } } *Excellent text. Every bio EM person should have this book. } } **Times for complete fixation in formalin is a } } common thread on the histonet mailing list. } } Usually grumping about how clinical turn-around } } times are too fast for proper/complete fixation. } } } } } } } Hi to all! } } } I am struggling with a professor because we have } } } 2 different opinions on the following problem: } } } We have a project involving the analysis of rat } } } organs both in histology and in EM. The } } } histology part only requires H&E staining, no } } } special labeling and no immunofluorescence. } } } No immunolabeling for EM neither. } } } Well the histologists want the whole organs to } } } be fixed for histology, then I could get a small } } } piece for EM. No problem for me. But they want } } } the organs fixed in formalin and I want them } } } fixed in Karnovsky (Formaldehyde + } } } glutaraldehyde). I wonder why it would not be } } } possible to fix the whole organs in } } } Karnovsky.ÝIs Karnovsky fixation a problem for } } } histology??! } } } I know glutaraldehyde should not be used for IF, } } } but we have no IF planned. I also know that } } } glutaradehyde can lead to overfixation } } } artifacts, but the material is being processed } } } immediately and not intended to be stored for a } } } long time. Another issue may be that } } } histologists do not wish to work with cacodylate } } } buffer, but they could simply change the } } } fixation medium once I got my piece of tissue. } } } I would appreciate your opinion and comments on } } } the feasibility of using Karnovsky as fixation } } } medium for histology. } } } I wish you all have such a wonderful shiny day as we have here in } } } Vienna. } } } Best regards, } } } } } } Stephane } } } } } } -- } -- } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583 } mcauliff-at-umdnj.edu } ********************************************** } } } } } } ==============================Original } Headers============================== } 10, 29 -- From mcauliff-at-umdnj.edu Thu May 8 09:09:16 2008 } 10, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU } [130.219.34.124]) } 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m48E9BGX018870 } 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 09:09:14 } -0500 } 10, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) } 10, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id } 11E48A7B8C } 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 } 10:09:11 -0400 (EDT) } 10, 29 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) } 10, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 2739DA7BB8 } 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 8 May 2008 } 10:09:10 -0400 (EDT) } 10, 29 -- Received: from ([10.32.15.171]) } 10, 29 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.150219744; } 10, 29 -- Thu, 08 May 2008 10:08:45 -0400 } 10, 29 -- Received: from [127.0.0.1] ([10.32.15.102]) } 10, 29 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging } Server 6.3-5.02 (built } 10, 29 -- Oct 12 2007; 32bit)) with ESMTP id {0K0J003QJZALIZI0-at-umduwc02.umdnj.edu } } for } 10, 29 -- microscopy-at-microscopy.com; Thu, 08 May 2008 10:08:45 } -0400 (EDT) } 10, 29 -- Date: Thu, 08 May 2008 10:09:23 -0400 } 10, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} } 10, 29 -- Subject: Re: [Microscopy] Re: karnovsky and histology } 10, 29 -- In-reply-to: {200805081343.m48DhSsH015349-at-ns.microscopy.com} } 10, 29 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com } 10, 29 -- Message-id: {48230993.6040507-at-umdnj.edu} } 10, 29 -- MIME-version: 1.0 } 10, 29 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed } 10, 29 -- References: {200805081343.m48DhSsH015349-at-ns.microscopy.com} } 10, 29 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) } 10, 29 -- Content-Transfer-Encoding: 8bit } 10, 29 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by } ns.microscopy.com id m48E9BGX018870 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 23 -- From Elliott-at-arizona.edu Thu May 8 19:09:27 2008 5, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4909RBr007578 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 19:09:27 -0500 5, 23 -- Received: from gandalfs_amavis (amavis8.email.arizona.edu [10.0.0.236]) 5, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2516A64ADF5 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 15:30:03 -0700 (MST) 5, 23 -- Received: from [150.135.145.124] (unknown [150.135.145.124]) 5, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 6759F64CA1B 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 8 May 2008 15:29:59 -0700 (MST) 5, 23 -- Message-Id: {7854C660-A518-45DE-B296-2F789D9FB66C-at-arizona.edu} 5, 23 -- From: David Elliott {Elliott-at-arizona.edu} 5, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 23 -- In-Reply-To: {200805081626.m48GQR8q004687-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 5, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) 5, 23 -- Subject: Re: [Microscopy] karnovsky and histology 5, 23 -- Date: Thu, 8 May 2008 15:29:57 -0700 5, 23 -- References: {200805081626.m48GQR8q004687-at-ns.microscopy.com} 5, 23 -- X-Mailer: Apple Mail (2.919.2) 5, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4909RBr007578 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 23 -- From kjmorris-at-well.ox.ac.uk Mon May 12 03:58:58 2008 17, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 17, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4C8wvGt028952 17, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 May 2008 03:58:57 -0500 17, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 17, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 17, 23 -- id 1JvTs1-0002bG-08 17, 23 -- for Microscopy-at-Microscopy.Com; Mon, 12 May 2008 09:58:57 +0100 17, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 17, 23 -- To: {Microscopy-at-Microscopy.Com} 17, 23 -- References: {200805090014.m490EmqZ021767-at-ns.microscopy.com} 17, 23 -- Subject: RE: [Microscopy] Re: karnovsky and histology 17, 23 -- Date: Mon, 12 May 2008 10:00:50 +0100 17, 23 -- Message-ID: {001501c8b40e$acdb3c90$b22c4381-at-CytoWhizz} 17, 23 -- MIME-Version: 1.0 17, 23 -- Content-Type: text/plain; 17, 23 -- charset="iso-8859-1" 17, 23 -- X-Mailer: Microsoft Office Outlook 11 17, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 17, 23 -- Thread-Index: AcixabI7EEjLSOYtRtid1pKCDGhKuAAU8icg 17, 23 -- In-Reply-To: {200805090014.m490EmqZ021767-at-ns.microscopy.com} 17, 23 -- Content-Transfer-Encoding: 8bit 17, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4C8wvGt028952 ==============================End of - Headers==============================
thanks to all who responded to my query } } What software are people using for 3D reconstructions from TEM? Line } tracing, surface rendering, segmentation and seed fill, EM } tomography.....
I was not aware of IMOD (tracing and 3D surface rendering) and Tomography. Other applications mentioned were Neurolucida and Amira both for tracing, rendering and analysis; and combinations of section alignment tools from Bitplane, tracing in ImageJ, homebrew seed fill, analysis in Volocity.
Looks like our current tools of ImageJ and Neurolucida will cover most of our needs, and I will try IMOD.
Thanks again, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 9, 24 -- From glenmac-at-u.washington.edu Mon May 12 10:25:32 2008 9, 24 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CFPVfw027153 9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 10:25:32 -0500 9, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 9, 24 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m4CFPcSG001176 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 08:25:38 -0700 9, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 9, 24 -- (authenticated authid=glenmac) 9, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m4CFPbAE000877 9, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 08:25:38 -0700 9, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 9, 24 -- Content-Transfer-Encoding: 7bit 9, 24 -- Message-Id: {7C388E17-B537-495F-86CC-74B8AEC5D8F8-at-u.washington.edu} 9, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 9, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 9, 24 -- Subject: TEM 3D software 9, 24 -- Date: Mon, 12 May 2008 08:25:36 -0700 9, 24 -- X-Mailer: Apple Mail (2.753) 9, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.12.81244 9, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_1000_1099 0, BODY_SIZE_5000_LESS 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Glen, I didn't see Imaris from Bitplane listed which is my current favorite particularly for confocal microscopy data but also for any stack of images. Larry
glenmac-at-u.washington.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Angel, } No, but that will be added to my list. } } http://www.cgl.ucsf.edu/chimera/ } } } Thanks, } Glen } On May 12, 2008, at 8:45 AM, Angel Paredes wrote: } } } Hi Glen, } } } } Did anyone mention chimera? } } } } angel paredes } } } } On Mon, 12 May 2008 glenmac-at-u.washington.edu wrote: } } } } } } } } } } } --------------------------------------------------------------------- } } } ------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } } MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } --------------------------------------------------------------------- } } } ------- } } } } } } thanks to all who responded to my query } } } } What software are people using for 3D reconstructions from TEM? } } } } Line } } } } tracing, surface rendering, segmentation and seed fill, EM } } } } tomography..... } } } I was not aware of IMOD (tracing and 3D surface rendering) and } } } Tomography. } } } Other applications mentioned were Neurolucida and Amira both for } } } tracing, rendering and analysis; and combinations of section } } } alignment tools from Bitplane, tracing in ImageJ, homebrew seed fill, } } } analysis in Volocity. } } } } } } Looks like our current tools of ImageJ and Neurolucida will cover } } } most of our needs, and I will try IMOD. } } } } } } Thanks again, } } } Glen } } } } } } } } } Glen MacDonald } } } Core for Communication Research } } } Virginia Merrill Bloedel Hearing Research Center } } } Box 357923 } } } University of Washington } } } Seattle, WA 98195-7923 USA } } } (206) 616-4156 } } } glenmac-at-u.washington.edu } } } } } } ********************************************************************* } } } *** } } } ****** } } } The box said "Requires WindowsXP or better", so I bought a Macintosh. } } } ********************************************************************* } } } *** } } } ****** } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 9, 24 -- From glenmac-at-u.washington.edu Mon May 12 10:25:32 2008 } } } 9, 24 -- Received: from mxout3.cac.washington.edu } } } (mxout3.cac.washington.edu [140.142.32.166]) } } } 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id m4CFPVfw027153 } } } 9, 24 -- for {microscopy-at-microscopy.com} ; 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-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 5, 28 -- From Larry.Ackerman-at-ucsf.edu Mon May 12 11:29:14 2008 5, 28 -- Received: from emfmcb02.ucsfmedicalcenter.org (EMFMCB02.ucsfmedicalcenter.org [64.54.46.98]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CGTDqp022169 5, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 11:29:13 -0500 5, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 5, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 5, 28 -- Mon, 12 May 2008 09:29:12 -0700 5, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 5, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 5, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Mon, 12 May 2008 09:29:25 -0700 5, 28 -- Message-ID: {48287064.2010204-at-ucsf.edu} 5, 28 -- Date: Mon, 12 May 2008 09:29:24 -0700 5, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 5, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 5, 28 -- Organization: UCSF, NeuroAnatomy 5, 28 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 5, 28 -- MIME-Version: 1.0 5, 28 -- To: Microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] Re: TEM 3D software 5, 28 -- References: {200805121559.m4CFxuiH019222-at-ns.microscopy.com} 5, 28 -- In-Reply-To: {200805121559.m4CFxuiH019222-at-ns.microscopy.com} 5, 28 -- X-OriginalArrivalTime: 12 May 2008 16:29:25.0024 (UTC) 5, 28 -- FILETIME=[574D8A00:01C8B44D] 5, 28 -- X-WSS-ID: 6436AFD229O13164500-01-01 5, 28 -- Content-Type: text/plain; 5, 28 -- charset=iso-8859-1; 5, 28 -- format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, I have had a problem accessing my Thumbs Plus Database (Cerious). I am using Pro version7 build 2251. When the problem started we tried to repair the database from outside the Thumbs program, but no one including our IS person had authorization to repair or compress the database. I managed to import the file as a text file, but only the older files that had been added with version4 were imported, and eventually wrote over the database even though I had saved it under another name. In the last three weeks I have tried contacting the company at least four times by internet support form and once by phone but have had no reply, so if anyone else has had this problem and came up with a solution please share. Thank-You, Pat Glazebrook
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==============================Original Headers============================== 7, 23 -- From pglazebrook-at-metrohealth.org Mon May 12 11:49:35 2008 7, 23 -- Received: from mfapp02.metrohealth.org (mfapp02.metrohealth.org [199.249.228.39]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CGnYp4002949 7, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 11:49:34 -0500 7, 23 -- Received: from mfapp02.metrohealth.org (127.0.0.1) by mfapp02.metrohealth.org (MlfMTA v3.2r9) id h51qho0171st for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 12:49:32 -0400 (envelope-from {pglazebrook-at-metrohealth.org} ) 7, 23 -- Received: from SNSMTP.metrohealth.org ([169.240.14.106]) 7, 23 -- by mfapp02.metrohealth.org (SonicWALL 6.1.1.9685) 7, 23 -- with ESMTP; Mon, 12 May 2008 12:49:32 -0400 7, 23 -- Received: from GWDSMTP-MTA by SNSMTP.metrohealth.org 7, 23 -- with Novell_GroupWise; Mon, 12 May 2008 12:49:35 -0400 7, 23 -- Message-Id: {48283CD30200002D00002692-at-SNSMTP.metrohealth.org} 7, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 7, 23 -- Date: Mon, 12 May 2008 12:49:23 -0400 7, 23 -- From: "Patricia Glazebrook" {pglazebrook-at-metrohealth.org} 7, 23 -- To: "microscopy" {Microscopy-at-microscopy.com} 7, 23 -- Subject: Problem with ThumbsPlus database 7, 23 -- Mime-Version: 1.0 7, 23 -- Content-Type: text/plain; charset=US-ASCII 7, 23 -- Content-Disposition: inline 7, 23 -- X-Mlf-Version: 6.1.1.9685 7, 23 -- X-Mlf-UniqueId: o200805121649320154239 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4CGnYp4002949 ==============================End of - Headers==============================
A five grid specimen holder was manufactured by JEOL for their 100CX 100CX II and 1200 series TEMs. I am trying to find one. JEOL-USA does not have one but is checking with Japan. Does anyone have one lying unused that I might obtain? Thanks, Larry -- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 3, 26 -- From Larry.Ackerman-at-ucsf.edu Mon May 12 12:51:35 2008 3, 26 -- Received: from emfmcb02.ucsfmedicalcenter.org (EMFMCB02.ucsfmedicalcenter.org [64.54.46.98]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CHpYuj017913 3, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 12:51:34 -0500 3, 26 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 3, 26 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 3, 26 -- Mon, 12 May 2008 10:51:15 -0700 3, 26 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 3, 26 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 3, 26 -- with Microsoft SMTPSVC(6.0.3790.3959); Mon, 12 May 2008 10:51:28 -0700 3, 26 -- Message-ID: {4828839E.5030806-at-ucsf.edu} 3, 26 -- Date: Mon, 12 May 2008 10:51:26 -0700 3, 26 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 3, 26 -- Reply-to: larry.ackerman-at-ucsf.edu 3, 26 -- Organization: UCSF, NeuroAnatomy 3, 26 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 3, 26 -- MIME-Version: 1.0 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Subject: JEOL five grid specimen holder 3, 26 -- X-OriginalArrivalTime: 12 May 2008 17:51:28.0181 (UTC) 3, 26 -- FILETIME=[CDBBAE50:01C8B458] 3, 26 -- X-WSS-ID: 64365C1929O13177974-01-01 3, 26 -- Content-Type: text/plain; 3, 26 -- charset=iso-8859-1; 3, 26 -- format=flowed 3, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
What is the problem's symptoms? One thing Thumbs does not always like are 16-bit files.
Every folder that has been accessed with Thumbs will have a thumbs.db file for that folder. This is copied to Program Files\Thumbs\thumbs.db when each folder is accessed. So, the actual database is in each sub-directory's folder.
You can open the .db with MS Access and see that it is OK. But you ought to be able to Repair the .db from inside Thumbs. Why does this not work?
gary g.
At 09:51 AM 5/12/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Mon May 12 13:02:20 2008 7, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4CI2Kp9027919 7, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 13:02:20 -0500 7, 20 -- Message-Id: {200805121802.m4CI2Kp9027919-at-ns.microscopy.com} 7, 20 -- Received: (qmail 18682 invoked from network); 12 May 2008 11:02:35 -0700 7, 20 -- Received: by simscan 1.1.0 ppid: 18679, pid: 18680, t: 0.1074s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by smtp1 with SMTP; 12 May 2008 11:02:35 -0700 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Mon, 12 May 2008 11:02:31 -0700 7, 20 -- To: pglazebrook-at-metrohealth.org 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] Problem with ThumbsPlus database 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200805121651.m4CGpZEK005574-at-ns.microscopy.com} 7, 20 -- References: {200805121651.m4CGpZEK005574-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-33056FA2 ==============================End of - Headers==============================
Single sided copper tape works fine. Also I have successfully used double sided copper tape on the aluminum stub instead of the double sided carbon tape.
Smitha.
donc-at-asmicro.com wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } What is a good method of attaching a silicon chip to an aluminum SEM stub, } so that: } a) the electrical contact is good, allowing high-resolution imaging (50 kx } or higher); and } b) the mechanical stability is good, so that the specimen can survive } shipping? } } I am aware of the following materials: } colloidal carbon in isopropyl alcohol } conductive carbon adhesive tabs } and I am curious about the experience of other microscopists. } } regards, } Don } ============================================= } Don Chernoff, Ph.D., President } Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com } 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 } INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) } web: http://www.asmicro.com Fax: 317-895-5652 } [business activities: analytical services in AFM, AFM probes, consulting, } training, } calibration and test specimens, calibration and measurement software, } used NanoScope equipment.] } ============================================= } } } ==============================Original Headers============================== } 4, 24 -- From donc-at-asmicro.com Fri May 9 16:40:21 2008 } 4, 24 -- Received: from smtp106.sbc.mail.re2.yahoo.com (smtp106.sbc.mail.re2.yahoo.com [68.142.229.99]) } 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m49LeLtp014758 } 4, 24 -- for {microscopy-at-microscopy.com} ; Fri, 9 May 2008 16:40:21 -0500 } 4, 24 -- Received: (qmail 67498 invoked from network); 9 May 2008 21:40:20 -0000 } 4, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-76.252.40.5 with login) } 4, 24 -- by smtp106.sbc.mail.re2.yahoo.com with SMTP; 9 May 2008 21:40:20 -0000 } 4, 24 -- X-YMail-OSG: o2ScAX0VM1neKwVGyKkYSnGG9KLdBGJ8.0BOmBx91NhekozvPv_ouy37Jadm5I9nF6G3Q1yP5VYRL94G5gcIvS6v5LIywl.rnMT.2FyexelNcEmPqmna_t39WNbVaz6zC_f3JsE2eIVvrtSkv9PgpXkH } 4, 24 -- X-Yahoo-Newman-Property: ymail-3 } 4, 24 -- Message-ID: {007801c8b21d$1f066e50$9b01a8c0-at-asm15} } 4, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} } 4, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} } 4, 24 -- Subject: Sample mounting } 4, 24 -- Date: Fri, 9 May 2008 17:39:09 -0400 } 4, 24 -- MIME-Version: 1.0 } 4, 24 -- Content-Type: text/plain; } 4, 24 -- format=flowed; } 4, 24 -- charset="iso-8859-1"; } 4, 24 -- reply-type=original } 4, 24 -- Content-Transfer-Encoding: 7bit } 4, 24 -- X-Priority: 3 } 4, 24 -- X-MSMail-Priority: Normal } 4, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 } 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 10, 32 -- From vvi-at-cypress.com Mon May 12 13:33:38 2008 10, 32 -- Received: from ns1.cypress.com (ns1.cypress.com [157.95.67.4]) 10, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CIXcdN012211 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 13:33:38 -0500 10, 32 -- Received: from corpmail.cypress.com (corpmail [157.95.1.2]) 10, 32 -- by ns1.cypress.com (8.12.10/8.12.10) with ESMTP id m4CIXoIU017928 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 11:33:50 -0700 (PDT) 10, 32 -- Received: from cobweb.mis.cypress.com (cobweb.mis.cypress.com [172.16.2.5]) 10, 32 -- by corpmail.cypress.com (8.12.10/8.12.10) with ESMTP id m4CIXi2U007423 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 11:33:45 -0700 (PDT) 10, 32 -- Received: from mailhost.cmi.cypress.com ([172.18.28.87]) 10, 32 -- by cobweb.mis.cypress.com (8.12.11/8.12.11) with ESMTP id m4CIXg7Q025506 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 11:33:42 -0700 (PDT) 10, 32 -- Received: from bitmap4.cmi.cypress.com (cmi [172.18.28.27]) 10, 32 -- by mailhost.cmi.cypress.com (8.13.4/8.13.4) with ESMTP id m4CIWw7o005783 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 13:32:58 -0500 10, 32 -- Received: from [172.18.23.63] ([172.18.23.63]) 10, 32 -- by bitmap4.cmi.cypress.com (8.11.7p3+Sun/8.11.6) with ESMTP id m4CIXaJ18327 10, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 12 May 2008 13:33:36 -0500 (CDT) 10, 32 -- Message-ID: {48288D82.5090509-at-cypress.com} 10, 32 -- Date: Mon, 12 May 2008 13:33:38 -0500 10, 32 -- From: Smitha Dronavalli {vvi-at-cypress.com} 10, 32 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923) 10, 32 -- X-Accept-Language: en-us, en 10, 32 -- MIME-Version: 1.0 10, 32 -- To: Microscopy-at-microscopy.com 10, 32 -- Subject: Re: [Microscopy] Sample mounting 10, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 32 -- Content-Transfer-Encoding: 7bit 10, 32 -- X-Cypress-MailScanner-Information: Please contact the ISP for more information 10, 32 -- X-Cypress-MailScanner: Found to be clean 10, 32 -- X-Cypress-MailScanner-From: vvi-at-cypress.com ==============================End of - Headers==============================
Vitaly mentioned the Kimball-Physics LaB6: on our heavily used CM12 - the predecessor to the Tecnai 12 - we used and are using Kimball-Physics LaB6 filaments - only three in the last 10 years. The vacuum of the TEM was (very) good, always, and still is .... I keep my fingers crossed. This means: I am a very satisfied customer ... and I do recommend this type. I have no relation to any company, neither FEI nor to Kimball-Physics.
Reinhard
--
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 6, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Mon May 12 13:58:20 2008 6, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CIwJG4025573 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 May 2008 13:58:19 -0500 6, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 6, 25 -- by localhost (Postfix) with SMTP id 1A8A27389E 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 May 2008 20:58:25 +0200 (CEST) 6, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 6, 25 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id 1358472A0B 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Mon, 12 May 2008 20:58:25 +0200 (CEST) 6, 25 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 6, 25 -- with Novell_GroupWise; Mon, 12 May 2008 20:58:16 +0200 6, 25 -- Message-Id: {4828AF61.044C.0054.0-at-biologie.uni-regensburg.de} 6, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 6, 25 -- Date: Mon, 12 May 2008 20:58:09 +0200 6, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 6, 25 -- To: {Microscopy-at-Microscopy.Com} 6, 25 -- Subject: Re: Tecnai12 filament life 6, 25 -- References: {200805110155.m4B1tF6d006378-at-ns.microscopy.com} 6, 25 -- In-Reply-To: {200805110155.m4B1tF6d006378-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=US-ASCII 6, 25 -- Content-Disposition: inline 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4CIwJG4025573 ==============================End of - Headers==============================
Does anybody have a schematic for a simple piranni gauge meter? I would like to plug it into my JSM-35C's piranni gauges to double-check the vacuum circuitry. The circuits aren't showing a vacuum, but the penning gauge is, although the vacuum is below the range of the penning gauge. I just want to be able to plug an alternate meter in to see what the vacuum is doing, relative to atmosphere. I know the pinout of the piranni head has one evacuated gauge tube for reference and an open tube for measuring, I just need to get a basic idea for the circuitry to build the meter part.
Or, if anybody has an extra piranni meter sitting around that isn't being used, I'd be interested in it.
Thanks,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 5, 27 -- From kraftpiano-at-gmail.com Mon May 12 14:41:38 2008 5, 27 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.249]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CJfcF7007249 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 14:41:38 -0500 5, 27 -- Received: by rv-out-0708.google.com with SMTP id k29so5482471rvb.30 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 12:41:37 -0700 (PDT) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=4jDM9m2xiR93wuk8VYKdIAbM1uqZNa/KCXe4/Z/hQII=; 5, 27 -- b=ORvYxdLHBtXKTuwYnyo+I2F/FBhQ5RJRKkzs94rPFPWL+X5C/nSUw0yR88IFCBQZbjh6Xh6qiAU8W8W51w54H8x0nqm68KQt5tJEJ7CrvZvKw7e1cuT64wWOi8YYnaCHP0o9aFpJRW7m0X9/opKefWI3sFMNNx0QcBaGURcPVcY= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=FE33ThF4V/dU/eFXQnFjC4MxcUq3JEOjiWSBlOkqrs+Aans2bmbrfAn9VmiAzX1ZsZhHEGiIoHd3xmRqUpLwwhqi7dfXCUqGExByCig2KrSdzO1psGqh9a4QC5JSnWpWSLT+kVJw0CW+dDquFkLdRten0FPi3mVX1FugvOGQYXM= 5, 27 -- Received: by 10.140.180.11 with SMTP id c11mr3857423rvf.137.1210621297818; 5, 27 -- Mon, 12 May 2008 12:41:37 -0700 (PDT) 5, 27 -- Received: by 10.140.161.11 with HTTP; Mon, 12 May 2008 12:41:37 -0700 (PDT) 5, 27 -- Message-ID: {25e2b0d20805121241v5d64a2a5q44ca497b6b98fc28-at-mail.gmail.com} 5, 27 -- Date: Mon, 12 May 2008 15:41:37 -0400 5, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Subject: Piranni gauge schematics 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
The question of the acceptable length of a rough pumping line has been pretty well covered by others who have already commented on this matter. However, if you want to see an actual calculation then you can find one on pages 44-45 of my book, "Vacuum Methods in Electron Microscopy"
If you do install a pumping line of considerably greater volume than the existing one, and if the pump-out valve is located at the end of this line nearest to the instrument, then you should give serious consideration to the phenomenon mentioned by Dr. Mardinly. That is, assume that the vacuum chamber is at atmospheric pressure, the pump-out valve is closed, and the pumping line is evacuated. Then if the pump-out valve is suddenly opened the sudden rush of air from the chamber into the rather large volume of the vacuum line could indeed cause a serious turbulence in the instrument chamber.
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 14 -- From bigelow-at-umich.edu Mon May 12 15:44:10 2008 3, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CKiAGk021900 3, 14 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 15:44:10 -0500 3, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 3, 14 -- BY hellskitchen.mr.itd.umich.edu ID 4828AC16.D9AD3.8420 ; 3, 14 -- 12 May 2008 16:44:06 -0400 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06240801c44e57bc4264-at-[141.212.131.221]} 3, 14 -- Date: Mon, 12 May 2008 16:44:04 -0400 3, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 14 -- Subject: [Microscopy]RE; Length of pump line 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I am helping some researchers get started on a project doing TEM of polytene chromosomes.
They have a paper describing the technique and everything looks familiar to me except one thing.
After EtOH dehydration, the paper describes using 'xylene extractions' before embedding the sample. It is at the same point in the prep that I would be thinking acetone or PO. I have not heard of using xylene at this stage, maybe someone could enlighten me.
Thanks
Jon --
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I am riding in the 2008 San Jose Livestrong Challenge to raise money to support victims of all forms of cancer. Go to http://www.livestrongchallenge.org to learn more. Start by choosing 'San Jose', then search for my name.
==============================Original Headers============================== 8, 20 -- From jmkrupp-at-ucsc.edu Mon May 12 18:48:26 2008 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4CNmQDJ009880 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 18:48:26 -0500 8, 20 -- Received: from [128.114.125.120] (HELO ucsc.edu) 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 8, 20 -- with ESMTPS id 34087239 for microscopy-at-microscopy.com; Mon, 12 May 2008 16:48:25 -0700 8, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 8, 20 -- with PIPE id 96385974; Mon, 12 May 2008 16:48:25 -0700 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu HELO [128.114.25.231]) 8, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 8, 20 -- with ESMTPA id 96385933 for microscopy-at-microscopy.com; Mon, 12 May 2008 16:48:17 -0700 8, 20 -- Mime-Version: 1.0 8, 20 -- Message-Id: {p06230909c44e867b3f87-at-[128.114.25.231]} 8, 20 -- Date: Mon, 12 May 2008 16:48:16 -0700 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 8, 20 -- Subject: 'Xylene extractions'? 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
From solicitorjrbriggs032-at-yahoo.co.uk Tue May 13 02:05:18 2008 Return-Path: {solicitorjrbriggs032-at-yahoo.co.uk} Received: from robbie.34sp.com (robbie.34sp.com [80.82.115.91]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4D75BOJ004048 for {microscopylistserverarchive-at-microscopy.com} ; Tue, 13 May 2008 02:05:11 -0500 Received: (qmail 1595 invoked from network); 13 May 2008 08:01:19 +0100 Received: from localhost (127.0.0.1) by localhost with SMTP; 13 May 2008 08:01:19 +0100 Received: from 196-220-6-118.netcomng.com (196-220-6-118.netcomng.com [196.220.6.118]) by webmail.nandhra.co.uk (Horde MIME library) with HTTP; Tue, 13 May 2008 08:01:17 +0100 Message-ID: {20080513080117.1cpbka7ne0owoooo-at-webmail.nandhra.co.uk}
some Kimball Physics LaB6 cathodes may be mechanically instable within first 50 or so hours of use, and gun tilt might need repetitive adjustment before cathode becomes stable. Worst case - cathode may have to be re-centered.
However, these cathodes take lots of punishment and still work for years. Leaky/dirty specimen airlock, fast heating/cooling of LaB6, vacuum accidents, arcing, etc. In other words multi-user environment with little supervision.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {reinhard.rachel-at-biologie.uni-regensburg.de} To: {vitalylazar-at-att.net} Sent: Monday, May 12, 2008 3:00 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ewgroth-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ewgroth-at-gmail.com Name: Eric W. Roth
Organization: NYU
Title-Subject: [Filtered] EM of Platelets
Question: Hello All,
I am trying to do some EM on mouse platelets. Does anyone have a good protocol on how to purify mouse platelets? Also, how much blood will I need in order to get a pellet? Any suggestions will be greatly appreciated.
We have a Jeol 5600LV and we are having an ongoing problem that Jeol say is an 'environmental issue'. We get interference on the images that manifest as bars down the image on the higher scan settings (this is a single solid bar on the right hand side of the image on scan 3, 3 bars on scan 4 across the whole image) These bars do not always seem to vary in their position with changes in KV and WD adjustments. Although the pronounced nature of the bars can be partially reduced by increasing the KV. on The effect on the image is was intermittent and now it seems to be permanently present. The lines represent as solid bars running vertically down the screen. Its not caused by vibration.
Essentially we want to find out if it is a field, we have had Jeol in to try and track down the field but with no success but they are adamant that there must be one despite being unable to locate it themselves. We don't really want to spend money on an expensive cancellation system if that is not the problem so has anyone out there had experience of:
(a) shielding microscopes cheaply in order to ascertain if it is a field. It doesn't mater if its really crude its just for initial diagnostic purposes. (b) similar problems with ageing microscopes indicating another possible fault? (c) any situation/equipment that may have produced similar effects.
Any idea would be great fully received as this is an ongoing problem that is currently confounding both us and Jeol alike. Thanks in advance. John
==============================Original Headers============================== 6, 27 -- From john.mitchels-at-gmail.com Tue May 13 07:35:01 2008 6, 27 -- Received: from hs-out-0708.google.com (hs-out-0708.google.com [64.233.178.242]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4DCZ0Bs005079 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 07:35:01 -0500 6, 27 -- Received: by hs-out-0708.google.com with SMTP id k27so2133166hsc.2 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 05:35:00 -0700 (PDT) 6, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 27 -- d=gmail.com; s=gamma; 6, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 27 -- bh=x/7eAJ0u2VPAnkjf59uapOzXeZik4CwqtIjJWGI/PWk=; 6, 27 -- b=rICl1kXJH6tlWXCKvg+NfPPBKegkdA9YiyGB8ecAKPmcg5YCmoylZhylpOfZexMOvkW0pAjzxgRncFl1TSwb8V6OjMBY1nLWDI0pNPIuWvP5s8P5XsL/KxXlLKTAZhgqwmks18kvXq12DHvmcfvwf8b+CLTmskMjVDax2oKpGpI= 6, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 27 -- d=gmail.com; s=gamma; 6, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 6, 27 -- b=UHkB7rfptWsQ9IadbOqNZvCX0PUzbHWWoa4uKPJGP/OLpBDAgwpW18AIuZBSC10+dljF1SdtMaceadUn/4axmpXdLApZj72dYcGVQxT/AKSkJnXHVmapCpnzxQrcorLgtxh29zgeWSwTLv8JDwx4zQIpXKkQzSZwFYBrHYFDfNo= 6, 27 -- Received: by 10.90.86.10 with SMTP id j10mr12210403agb.104.1210682100366; 6, 27 -- Tue, 13 May 2008 05:35:00 -0700 (PDT) 6, 27 -- Received: by 10.90.90.17 with HTTP; Tue, 13 May 2008 05:35:00 -0700 (PDT) 6, 27 -- Message-ID: {1b3cf7c30805130535wd010b82l99490ed984b58571-at-mail.gmail.com} 6, 27 -- Date: Tue, 13 May 2008 13:35:00 +0100 6, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 6, 27 -- To: microscopy-at-microscopy.com 6, 27 -- Subject: Interference on an SEM 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; charset=ISO-8859-1 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
We are planning to get rid of our old Zeiss EM10C TEM and would like to know if there is still an interest out there for this old type of microscope. If so, you may have the whole instrument or parts from it for free, as long as you come here (Alnarp, Sweden) to pick it/them up. (In case of very small parts, we might consider sending them at the addressee’s expense.)
The offer also includes the following accessories: 1. Rotary pump (Alcatel) 2. Water cooler (Van der Heiden Kühlmobil) 3. Vacuum evaporator (JEOL JEE-4X) 4. LKB 8800 Ultrotome III 5. LKB 2168 Ultrostainer Carlsberg System 6. Two service manuals in English for Zeiss EM10C 7. Enlarger (Durst Laborator 1200) for various film sizes
Best regards, Kerstin Brismar
****************************** Kerstin Brismar B.Sc., Research engineer, Photographer EM Unit, Dept. of Plant Protection Biology SLU (Swedish University of Agricultural Sciences) P.O. Box 102 (Delivery: Växtskyddsvägen 1) SE-230 53 Alnarp, Sweden Phone: +46 40 41 55 05 Fax: +46 40 41 55 19 E-mail: Kerstin.Brismar-at-ltj.slu.se http://www.ltj.slu.se/2/O2_spe1.html
******************************
==============================Original Headers============================== 9, 17 -- From Kerstin.Brismar-at-ltj.slu.se Tue May 13 09:33:02 2008 9, 17 -- Received: from alnus.slu.se (alnus.slu.se [194.47.49.5]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4DEX02n030354 9, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 13 May 2008 09:33:01 -0500 9, 17 -- Received: from vv-238.ltj.slu.se (vv-238.vv.slu.se [194.47.228.116]) 9, 17 -- by alnus.slu.se (8.13.7/8.13.7) with ESMTP id m4DEWxe5020951 9, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 13 May 2008 16:32:59 +0200 (CEST) 9, 17 -- Message-Id: {200805131432.m4DEWxe5020951-at-alnus.slu.se} 9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 17 -- Date: Tue, 13 May 2008 16:32:46 +0200 9, 17 -- To: Microscopy-at-Microscopy.Com 9, 17 -- From: Kerstin Brismar {Kerstin.Brismar-at-ltj.slu.se} 9, 17 -- Subject: TEM - SPARE PARTS OPPORTUNITY ! 9, 17 -- Mime-Version: 1.0 9, 17 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 9, 17 -- Content-Transfer-Encoding: 8bit 9, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4DEX02n030354 ==============================End of - Headers==============================
Could that be to extract lipids and/or some other stuff (polytene chromosomes are prepared from some insect, right?) and thus "clarify" the EM view? Just a guess...
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Begin forwarded message:
} From: jmkrupp-at-ucsc.edu } Date: May 12, 2008 7:49:33 PM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] 'Xylene extractions'? } Reply-To: jmkrupp-at-ucsc.edu } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi: } } I am helping some researchers get started on a project doing TEM of } polytene chromosomes. } } They have a paper describing the technique and everything looks } familiar to me except one thing. } } After EtOH dehydration, the paper describes using 'xylene } extractions' before embedding the sample. It is at the same point in } the prep that I would be thinking acetone or PO. I have not heard of } using xylene at this stage, maybe someone could enlighten me. } } Thanks } } Jon } -- } } Jonathan Krupp } Microscopy & Imaging Lab } C230 Earth & Marine Science } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-ucsc.edu } } I am riding in the 2008 San Jose Livestrong Challenge to raise money } to support victims of all forms of cancer. Go to } http://www.livestrongchallenge.org to learn more. Start by choosing } 'San Jose', then search for my name. } } ==============================Original } Headers============================== } 8, 20 -- From jmkrupp-at-ucsc.edu Mon May 12 18:48:26 2008 } 8, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m4CNmQDJ009880 } 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 12 May 2008 } 18:48:26 -0500 } 8, 20 -- Received: from [128.114.125.120] (HELO ucsc.edu) } 8, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) } 8, 20 -- with ESMTPS id 34087239 for microscopy-at-microscopy.com; } Mon, 12 May 2008 16:48:25 -0700 } 8, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro } PIPE 5.1.11) } 8, 20 -- with PIPE id 96385974; Mon, 12 May 2008 16:48:25 -0700 } 8, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) } 8, 20 -- Received: from [128.114.25.231] (account jmkrupp-at-ucsc.edu } HELO [128.114.25.231]) } 8, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) } 8, 20 -- with ESMTPA id 96385933 for microscopy-at-microscopy.com; } Mon, 12 May 2008 16:48:17 -0700 } 8, 20 -- Mime-Version: 1.0 } 8, 20 -- Message-Id: {p06230909c44e867b3f87-at-[128.114.25.231]} } 8, 20 -- Date: Mon, 12 May 2008 16:48:16 -0700 } 8, 20 -- To: microscopy-at-microscopy.com } 8, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} } 8, 20 -- Subject: 'Xylene extractions'? } 8, 20 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 23 -- From vladislav_speransky-at-nih.gov Tue May 13 12:40:18 2008 7, 23 -- Received: from nihrelayxway5.hub.nih.gov (nihrelayxway5.hub.nih.gov [128.231.90.99]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4DHeIWR018721 7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 13 May 2008 12:40:18 -0500 7, 23 -- X-IronPortListener: NIH_Relay 7, 23 -- X-SBRS: None 7, 23 -- X-IronPort-AV: E=Sophos;i="4.27,480,1204520400"; 7, 23 -- d="scan'208";a="176013452" 7, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 7, 23 -- by nihrelayxway5.hub.nih.gov with ESMTP; 13 May 2008 13:40:17 -0400 7, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 7, 23 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m4DHeHig12893866 7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 13 May 2008 13:40:17 -0400 (EDT) 7, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 7, 23 -- References: {200805122349.m4CNnXTW011298-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 23 -- Message-Id: {1D9C1EBB-4C3D-42CA-9AC2-76FFB718FDE5-at-nih.gov} 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 7, 23 -- Subject: Fwd: [Microscopy] 'Xylene extractions'? 7, 23 -- Date: Tue, 13 May 2008 13:39:36 -0400 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
I would think that there was a mistake in writing up the Materials and Methods section of the paper. My guess is that PO was used. 'Xlyene extractions' sounds too much like LM.
In the past when something in a paper sounded strange I have contacted the author and asked to have the EM person call me back (now email). In this way I could ask exactly what was done, not what the author thought was done and the procedure was usually as I expected. Sometimes papers get submitted without the technical staff being asked to proof read them or at least the Methods section. Happened to me once and never again!
Regards, Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} ---
} I am helping some researchers get started on a project doing TEM of } polytene chromosomes. } } They have a paper describing the technique and everything looks } familiar to me except one thing. } } After EtOH dehydration, the paper describes using 'xylene } extractions' before embedding the sample. It is at the same point in } the prep that I would be thinking acetone or PO. I have not heard of } using xylene at this stage, maybe someone could enlighten me. } } Jonathan Krupp } Microscopy & Imaging Lab } C230 Earth & Marine Science } University of California } Santa Cruz, CA 95064 } (831) 459-2477 } jmkrupp-at-ucsc.edu
==============================Original Headers============================== 8, 27 -- From connellyps-at-nhlbi.nih.gov Tue May 13 14:33:52 2008 8, 27 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4DJXpKk004686 8, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 14:33:51 -0500 8, 27 -- X-IronPortListener: Outbound_SMTP 8, 27 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 8, 27 -- by nihxway5out.hub.nih.gov with ESMTP; 13 May 2008 15:33:51 -0400 8, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 8, 27 -- Tue, 13 May 2008 15:33:46 -0400 8, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.165]) with Microsoft Exchange Server HTTP-DAV ; 8, 27 -- Tue, 13 May 2008 19:33:46 +0000 8, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 8, 27 -- Date: Tue, 13 May 2008 15:30:15 -0400 8, 27 -- Subject: Re: [Microscopy] 'Xylene extractions'? 8, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 8, 27 -- To: {jmkrupp-at-ucsc.edu} , 8, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 27 -- Message-ID: {C44F6487.1C4C%connellyps-at-nhlbi.nih.gov} 8, 27 -- Thread-Topic: [Microscopy] 'Xylene extractions'? 8, 27 -- Thread-Index: Aci1L8TOAyV5GSEjEd2S1QANk2Yv1A== 8, 27 -- In-Reply-To: {200805122353.m4CNrxei017714-at-ns.microscopy.com} 8, 27 -- Mime-version: 1.0 8, 27 -- Content-type: text/plain; 8, 27 -- charset="ISO-8859-1" 8, 27 -- X-OriginalArrivalTime: 13 May 2008 19:33:46.0468 (UTC) FILETIME=[42D9B240:01C8B530] 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4DJXpKk004686 ==============================End of - Headers==============================
Dear all, I have been asked to found the best method to freeze entire rat organs for an undetermined period of time, maybe years. They may be recovered any time for histology observation. I told the people that it was probably better to leave them in a fixative at 4°C but they want them frozen. I am not very experienced in cryopreservation (except for cells in culture), especially of whole organs. For me the ideal way would be to perfuse with glycerol (I seem to remember something like 60%) but I simply wonder if we master the technique to cryopreserve whole rat organs. Fact is, here we need only to maintain the structure, not the activity! Problem is, the organs cannot be perfused. They will be fixed in formalin and given to me whole. If anyone has experience or infos to share, they are welcome. Stephane
P.S: don't laugh: they just wanted to quick-freeze them by directly plunging the organs in liquid nitrogen!
==============================Original Headers============================== 5, 21 -- From nizets2-at-yahoo.com Wed May 14 04:58:21 2008 5, 21 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4E9wJBu013979 5, 21 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2008 04:58:20 -0500 5, 21 -- Received: (qmail 52964 invoked by uid 60001); 14 May 2008 09:58:18 -0000 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 21 -- s=s1024; d=yahoo.com; 5, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 21 -- b=Ugb9vRZAp8URzwCI3wPnghgAYSea9wVL7Ns9i23nrZtzYMfvs8axWVV0CnBNS+Q7ujDZPm8ab9CLkBivhz8DOfRup4644oJHeVLiiWgPGecRP9Q1MdqHtTJn0t8Gej7IE31WJtYnsAiN+nqmx3uAF0mNTY6pPpiIWlw0qT1O5AM=; 5, 21 -- X-YMail-OSG: r.1ABgkVM1nSNshliAQjRtqrpjanTBdVflfVjg59iUVM4_mafkpP66FQpKMn7PN1HppsueOzhNCaMjanTQLz.axgQShfpB2rJF2jZw-- 5, 21 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Wed, 14 May 2008 02:58:18 PDT 5, 21 -- X-Mailer: YahooMailRC/975.23 YahooMailWebService/0.7.185 5, 21 -- Date: Wed, 14 May 2008 02:58:18 -0700 (PDT) 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 21 -- Subject: =?iso-8859-1?Q?Storage_of_organs_at_-=B0C?= 5, 21 -- To: microscopy-at-microscopy.com 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 5, 21 -- Message-ID: {636315.52694.qm-at-web37408.mail.mud.yahoo.com} 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4E9wJBu013979 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ederoever-at-nalco.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ederoever-at-nalco.com Name: Emond de Roever
Organization: Nalco Europe BV
Title-Subject: [Filtered] Image blurring due to Oxford EDS detector 7117 in ESEM
Question: I would like to know whether ESEM-2020 and other ESEM users ever experienced image-interference problems caused by an Oxford 7117 detector. If my ("new") 7117 detector is placed too near to the sample (and beam) the image becomes fuzzy and blurred, due to the presence of a small magnet in the nose of the detector. So the detector has to be placed several cm away from the sample, to prevent this fuzziness. This is not optimal for EDS. Is this blurring normal for 7117 detectors placed close to the sample and is this a familiar problem to ESEM users? Or is the magnet in this particular detector malfunctioning and/or not working properly? I recently got this demo 7117 detector after having used another detector (not 7117) for over 10 years without problems, on my ESEM-2020. I did not get a clear reply from Oxford, they said it should not be a problem.
I am assisting a German company in their search for potential end-users of their Large Chamber SEM for the CEO to meet with end of this month/beginning of June.
The SEM can handle sample sizes up to 35 cubic FEET in volume, so samples do not need to be destroyed or broken down to fit a standard SEM. System is already used at Tinker AFB and Y-12 National Security Complex.
Does anyone know who the target group would be for this? I've tried Universities with no luck, as they require smaller, not larger SEMs. What would the potential applications be for this outside of Aerospace? Perhaps museums? Thank you for your guidance!
Travis Biggs German-American Chamber of Commerce tbiggs-at-gaccsouth.com
==============================Original Headers============================== 9, 22 -- From tbiggs-at-gaccsouth.com Wed May 14 08:48:58 2008 9, 22 -- Received: from mail.gaccsouth.com (74.223.164.194.nw.nuvox.net [74.223.164.194]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4EDmwoO022365 9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2008 08:48:58 -0500 9, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 22 -- Content-class: urn:content-classes:message 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; 9, 22 -- charset="us-ascii" 9, 22 -- Subject: German Large Chamber SEM 9, 22 -- Date: Wed, 14 May 2008 09:48:58 -0400 9, 22 -- Message-ID: {57439DEBA7E4EB4DA2CF17F69B3EF4F0DDB9CD-at-gaccsouth-dc01.gaccsouth.atl} 9, 22 -- In-Reply-To: {200805141335.m4EDZvSC021812-at-ns.microscopy.com} 9, 22 -- X-MS-Has-Attach: 9, 22 -- X-MS-TNEF-Correlator: 9, 22 -- Thread-Topic: German Large Chamber SEM 9, 22 -- Thread-Index: Aci1x3S6FaO/HrJnTiyy1HxyN+3o1gAAYpvw 9, 22 -- References: {200805141335.m4EDZvSC021812-at-ns.microscopy.com} 9, 22 -- From: "Travis Biggs" {tbiggs-at-gaccsouth.com} 9, 22 -- To: {microscopy-at-microscopy.com} 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4EDmwoO022365 ==============================End of - Headers==============================
Travis, I doubt most museums would have the budget for something like that. NTSB (National Transportation Safety Board) and FBI are the only ones that come to mind outside of aerospace companies and NASA.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: tbiggs-at-gaccsouth.com [mailto:tbiggs-at-gaccsouth.com] Sent: Wednesday, May 14, 2008 9:52 AM To: kenconverse-at-qualityimages.biz
Hello everyone,
I am assisting a German company in their search for potential end-users of their Large Chamber SEM for the CEO to meet with end of this month/beginning of June.
The SEM can handle sample sizes up to 35 cubic FEET in volume, so samples do not need to be destroyed or broken down to fit a standard SEM. System is already used at Tinker AFB and Y-12 National Security Complex.
Does anyone know who the target group would be for this? I've tried Universities with no luck, as they require smaller, not larger SEMs. What would the potential applications be for this outside of Aerospace? Perhaps museums? Thank you for your guidance!
Travis Biggs German-American Chamber of Commerce tbiggs-at-gaccsouth.com
==============================Original Headers============================== 9, 22 -- From tbiggs-at-gaccsouth.com Wed May 14 08:48:58 2008 9, 22 -- Received: from mail.gaccsouth.com (74.223.164.194.nw.nuvox.net [74.223.164.194]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4EDmwoO022365 9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2008 08:48:58 -0500 9, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 22 -- Content-class: urn:content-classes:message 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; 9, 22 -- charset="us-ascii" 9, 22 -- Subject: German Large Chamber SEM 9, 22 -- Date: Wed, 14 May 2008 09:48:58 -0400 9, 22 -- Message-ID: {57439DEBA7E4EB4DA2CF17F69B3EF4F0DDB9CD-at-gaccsouth-dc01.gaccsouth.atl} 9, 22 -- In-Reply-To: {200805141335.m4EDZvSC021812-at-ns.microscopy.com} 9, 22 -- X-MS-Has-Attach: 9, 22 -- X-MS-TNEF-Correlator: 9, 22 -- Thread-Topic: German Large Chamber SEM 9, 22 -- Thread-Index: Aci1x3S6FaO/HrJnTiyy1HxyN+3o1gAAYpvw 9, 22 -- References: {200805141335.m4EDZvSC021812-at-ns.microscopy.com} 9, 22 -- From: "Travis Biggs" {tbiggs-at-gaccsouth.com} 9, 22 -- To: {microscopy-at-microscopy.com} 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4EDmwoO022365 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From kenconverse-at-qualityimages.biz Wed May 14 09:08:59 2008 21, 25 -- Received: from mta11.adelphia.net (mta11.adelphia.net [68.168.78.205]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4EE8xMn003513 21, 25 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 09:08:59 -0500 21, 25 -- Received: from Ken ([72.227.100.25]) by mta11.adelphia.net 21, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 21, 25 -- id {20080514140736.XYLL15333.mta11.adelphia.net-at-Ken} ; 21, 25 -- Wed, 14 May 2008 10:07:36 -0400 21, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 25 -- To: {tbiggs-at-gaccsouth.com} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 21, 25 -- Subject: RE: [Microscopy] German Large Chamber SEM 21, 25 -- Date: Wed, 14 May 2008 10:08:39 -0400 21, 25 -- Message-ID: {000201c8b5cc$02b4fa70$6401a8c0-at-Ken} 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="us-ascii" 21, 25 -- X-Priority: 3 (Normal) 21, 25 -- X-MSMail-Priority: Normal 21, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 21, 25 -- Importance: Normal 21, 25 -- Thread-Index: Aci1ya3cwXqeBFlVROO2A6dtNSBmuQAAbsQg 21, 25 -- In-Reply-To: {200805141351.m4EDpxqs026593-at-ns.microscopy.com} 21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4EE8xMn003513 ==============================End of - Headers==============================
Travis, I cannot resist asking how long it takes to pump down that cavernous chamber? And whether you all make a sputter coater for such mammoth samples???
Thanks, Tobias
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
John; External magnetic field interference is always worse with lower KV and longer working distance. It usually manifests itself as line to line displacement of the image. There should also be a way to synch or unsynch the raster to 60 cycle to see it get worse. If none of these behaviors are evident, it is probably not an external field. As far as chasing down the source goes, the simple Extech 480823 that you can now get at Amazon.com for $99 http://www.amazon.com/gp/product/B00023RXDC will enable you to find the field and measure it easily. Another thing to look at is very dirty power. I'm not sure how to measure that, but I'm sure your local electrician can. In that case, a UPS or isolation transformer can solve the problem. Of course, the microscopy power supply should have some giant capacitors to do a significant amount of isolation, and maybe JEOL needs to look at that. If your SEM is old, capacitors could easily be not performing properly.
John Mardinly, Numonyx
-----Original Message----- X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com] Sent: Tuesday, May 13, 2008 5:42 AM To: MARDINLY, A
Dear Listers
We have a Jeol 5600LV and we are having an ongoing problem that Jeol say is an 'environmental issue'. We get interference on the images that manifest as bars down the image on the higher scan settings (this is a single solid bar on the right hand side of the image on scan 3, 3 bars on scan 4 across the whole image) These bars do not always seem to vary in their position with changes in KV and WD adjustments. Although the pronounced nature of the bars can be partially reduced by increasing the KV. on The effect on the image is was intermittent and now it seems to be permanently present. The lines represent as solid bars running vertically down the screen. Its not caused by vibration.
Essentially we want to find out if it is a field, we have had Jeol in to try and track down the field but with no success but they are adamant that there must be one despite being unable to locate it themselves. We don't really want to spend money on an expensive cancellation system if that is not the problem so has anyone out there had experience of:
(a) shielding microscopes cheaply in order to ascertain if it is a field. It doesn't mater if its really crude its just for initial diagnostic purposes. (b) similar problems with ageing microscopes indicating another possible fault? (c) any situation/equipment that may have produced similar effects.
Any idea would be great fully received as this is an ongoing problem that is currently confounding both us and Jeol alike. Thanks in advance. John
==============================Original Headers============================== 6, 27 -- From john.mitchels-at-gmail.com Tue May 13 07:35:01 2008 6, 27 -- Received: from hs-out-0708.google.com (hs-out-0708.google.com [64.233.178.242]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4DCZ0Bs005079 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 07:35:01 -0500 6, 27 -- Received: by hs-out-0708.google.com with SMTP id k27so2133166hsc.2 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 05:35:00 -0700 (PDT) 6, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 27 -- d=gmail.com; s=gamma; 6, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 6, 27 -- bh=x/7eAJ0u2VPAnkjf59uapOzXeZik4CwqtIjJWGI/PWk=; 6, 27 -- b=rICl1kXJH6tlWXCKvg+NfPPBKegkdA9YiyGB8ecAKPmcg5YCmoylZhylpOfZexMOvkW0pA jzxgRncFl1TSwb8V6OjMBY1nLWDI0pNPIuWvP5s8P5XsL/KxXlLKTAZhgqwmks18kvXq12DH vmcfvwf8b+CLTmskMjVDax2oKpGpI= 6, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 27 -- d=gmail.com; s=gamma; 6, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer-encoding:content-disposition; 6, 27 -- b=UHkB7rfptWsQ9IadbOqNZvCX0PUzbHWWoa4uKPJGP/OLpBDAgwpW18AIuZBSC10+dljF1S dtMaceadUn/4axmpXdLApZj72dYcGVQxT/AKSkJnXHVmapCpnzxQrcorLgtxh29zgeWSwTLv 8JDwx4zQIpXKkQzSZwFYBrHYFDfNo= 6, 27 -- Received: by 10.90.86.10 with SMTP id j10mr12210403agb.104.1210682100366; 6, 27 -- Tue, 13 May 2008 05:35:00 -0700 (PDT) 6, 27 -- Received: by 10.90.90.17 with HTTP; Tue, 13 May 2008 05:35:00 -0700 (PDT) 6, 27 -- Message-ID: {1b3cf7c30805130535wd010b82l99490ed984b58571-at-mail.gmail.com} 6, 27 -- Date: Tue, 13 May 2008 13:35:00 +0100 6, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} 6, 27 -- To: microscopy-at-microscopy.com 6, 27 -- Subject: Interference on an SEM 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; charset=ISO-8859-1 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From A.MARDINLY-at-numonyx.com Wed May 14 11:13:38 2008 16, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4EGDbhE002304 16, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 11:13:37 -0500 16, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 16, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 6AA701E81F2; 16, 29 -- Wed, 14 May 2008 10:40:36 -0400 (EDT) 16, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 16, 29 -- Wed, 14 May 2008 12:13:37 -0400 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 29 -- Content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="us-ascii" 16, 29 -- Subject: RE: [Microscopy] Interference on an SEM 16, 29 -- Date: Wed, 14 May 2008 12:13:36 -0400 16, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF939A2C2-at-EXDRESBENMX012.numonyx.local} 16, 29 -- In-Reply-To: {200805131241.m4DCfcJD024853-at-ns.microscopy.com} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] Interference on an SEM 16, 29 -- Thread-Index: Aci09sbobdvFBddkTcSslOLyk+vmXwA5UVPQ 16, 29 -- References: {200805131241.m4DCfcJD024853-at-ns.microscopy.com} 16, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 16, 29 -- To: {john.mitchels-at-gmail.com} 16, 29 -- Cc: {Microscopy-at-MSA.Microscopy.Com} 16, 29 -- X-OriginalArrivalTime: 14 May 2008 16:13:37.0280 (UTC) FILETIME=[773AC800:01C8B5DD] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4EGDbhE002304 ==============================End of - Headers==============================
I myself haven't had experience with (or real need of) such a mammoth SEM, so I can only offer second-hand information. Several of our UTEP Metallurgy and Materials Engineering alumni now work at a facility with one of these instruments, and I have had a few conversations with some of them about this new instrument.
The large chamber would be of value when doing some failure investigation work, in which one needs to find or assess damage (corrosion, microcracks, porosity, etc.) over the surface of a large part. If the microscopist has to cut the part into tiny pieces to get it into the chamber, the damage caused by holding, handling, and cutting can destroy important evidence, or introduce misleading artifacts. If litigation looms, this concern is amplified.
A giant chamber also might allow SEM microscopy as a nondestructive evaluation method in periodic depot maintenance of big machinery. It could allow the engineering staff to better assess the severity of wear and tear or damage on a large part, and to make better-informed decisions about whether the deterioration is severe enough to remove the part from service. Many times we need to keep older equipment in service, even after the production lines have been dismantled and replacement parts are very difficult to obtain. (This consideration might apply to marine and railroad equipment, aircraft, refinery and chemical-plant equipment, etc.--any heavy industry where expensive capital needs to be carefully monitored for a long service life.)
So, as potential buyers of this type of instrument, you might look to the largest failure investigation firms, and big (captive or job-shop) depot-maintenance and refurbishment companes that serve heavy industry. (Plus Government labs.)
You say it is an ongoing problem, but give me the impression they were not always there. You also indicate they were intermittent when they first started appearing, and are always present, now.
How long ago did they first start to appear? Have you had the system for a while, where it performed nicely, before the current symptom started to appear? (you do say "aging microscope" near the bottom) Has there been any new construction, or equipment installations, in the vicinity of your system? Was there a repair, parts replaced, on your system? If so, is there a relationship, time wise?
First, to answer your question about shielding, there is no effective way to easily and cheaply block outside fields. Even expensive Active Isolation systems have their limitations.
Are these bars directly vertical, or do they angle down across the screen? If the bars look basically the same at 15kV and 4mm working distance, as they do at 1kV and 15mm working distance, then it is probably from with in the system. If Jeol cannot "find", and show you the "environmental" influence, then it is probably not there. You should be able to pick up, and see, a field that is strong enough to influence the beam that much. If the bars are vertical, then I find it difficult to believe an outside field would be that synchronous with the scan of the SEM.
I had a Jeol 6400F SEM that had a problem from the start. Jeol had pre-checked the room for fields and vibrations, and had given the room their blessing. After the system arrived, and was set up, there was an intermittent quirk in the image. Jeol could not figure it out, or stop it, so they decided it was from fields outside the system. They could not detect them, or show them to me, but they must be there! After I had relocated the system to a different building, several miles away from the first, to a pretested and approved room, I still had the exact same symptoms. (Just to be clear, my symptoms were different than yours) They thoroughly checked the system again, and then brought in an active field and vibration blocking system, thinking they were going to prove it was the environment, and not the system. They were wrong.
If they can't detect, and see the environmental fields, I don't believe they are affecting your system.
Can you answer any of the questions above? (Or, did you already answer those questions for yourself?)
Darrell
john.mitchels-at-gmail.com wrote on 05/13/2008 07:35:37 AM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } We have a Jeol 5600LV and we are having an ongoing problem that Jeol } say is an 'environmental issue'. We get interference on the images } that manifest as bars down the image on the higher scan settings (this } is a single solid bar on the right hand side of the image on scan 3, 3 } bars on scan 4 across the whole image) These bars do not always seem } to vary in their position with changes in KV and WD adjustments. } Although the pronounced nature of the bars can be partially reduced by } increasing the KV. on The effect on the image is was intermittent and } now it seems to be permanently present. The lines represent as solid } bars running vertically down the screen. Its not caused by vibration. } } Essentially we want to find out if it is a field, we have had Jeol in } to try and track down the field but with no success but they are } adamant that there must be one despite being unable to locate it } themselves. We don't really want to spend money on an expensive } cancellation system if that is not the problem so has anyone out there } had experience of: } } (a) shielding microscopes cheaply in order to ascertain if it is a } field. It doesn't mater if its really crude its just for initial } diagnostic purposes. } (b) similar problems with ageing microscopes indicating another } possible fault? } (c) any situation/equipment that may have produced similar effects. } } } Any idea would be great fully received as this is an ongoing problem } that is currently confounding both us and Jeol alike. } Thanks in advance. } John } } ==============================Original Headers============================== } 6, 27 -- From john.mitchels-at-gmail.com Tue May 13 07:35:01 2008 } 6, 27 -- Received: from hs-out-0708.google.com (hs-out-0708.google. } com [64.233.178.242]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m4DCZ0Bs005079 } 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 07:35:01 -0500 } 6, 27 -- Received: by hs-out-0708.google.com with SMTP id k27so2133166hsc.2 } 6, 27 -- for {microscopy-at-microscopy.com} ; Tue, 13 May 2008 } 05:35:00 -0700 (PDT) } 6, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 27 -- d=gmail.com; s=gamma; } 6, 27 -- h=domainkey-signature:received:received:message-id: } date:from:to:subject:mime-version:content-type:content-transfer- } encoding:content-disposition; } 6, 27 -- bh=x/7eAJ0u2VPAnkjf59uapOzXeZik4CwqtIjJWGI/PWk=; } 6, 27 -- } b=rICl1kXJH6tlWXCKvg+NfPPBKegkdA9YiyGB8ecAKPmcg5YCmoylZhylpOfZexMOvkW0pAjzxgRncFl1TSwb8V6OjMBY1nLWDI0pNPIuWvP5s8P5XsL/KxXlLKTAZhgqwmks18kvXq12DHvmcfvwf8b+CLTmskMjVDax2oKpGpI= } 6, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 6, 27 -- d=gmail.com; s=gamma; } 6, 27 -- h=message-id:date:from:to:subject:mime-version: } content-type:content-transfer-encoding:content-disposition; } 6, 27 -- } b=UHkB7rfptWsQ9IadbOqNZvCX0PUzbHWWoa4uKPJGP/OLpBDAgwpW18AIuZBSC10+dljF1SdtMaceadUn/4axmpXdLApZj72dYcGVQxT/AKSkJnXHVmapCpnzxQrcorLgtxh29zgeWSwTLv8JDwx4zQIpXKkQzSZwFYBrHYFDfNo= } 6, 27 -- Received: by 10.90.86.10 with SMTP id j10mr12210403agb.104. } 1210682100366; } 6, 27 -- Tue, 13 May 2008 05:35:00 -0700 (PDT) } 6, 27 -- Received: by 10.90.90.17 with HTTP; Tue, 13 May 2008 05:35: } 00 -0700 (PDT) } 6, 27 -- Message-ID: } {1b3cf7c30805130535wd010b82l99490ed984b58571-at-mail.gmail.com} } 6, 27 -- Date: Tue, 13 May 2008 13:35:00 +0100 } 6, 27 -- From: "John Mitchels" {john.mitchels-at-gmail.com} } 6, 27 -- To: microscopy-at-microscopy.com } 6, 27 -- Subject: Interference on an SEM } 6, 27 -- MIME-Version: 1.0 } 6, 27 -- Content-Type: text/plain; charset=ISO-8859-1 } 6, 27 -- Content-Transfer-Encoding: 7bit } 6, 27 -- Content-Disposition: inline } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 28 -- From milesd-at-us.ibm.com Wed May 14 13:38:45 2008 13, 28 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4EIci2K000901 13, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 13:38:45 -0500 13, 28 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 13, 28 -- by e6.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id m4EIesTP028804 13, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 14:40:54 -0400 13, 28 -- Received: from d01av04.pok.ibm.com (d01av04.pok.ibm.com [9.56.224.64]) 13, 28 -- by d01relay04.pok.ibm.com (8.13.8/8.13.8/NCO v8.7) with ESMTP id m4EIchOn130082 13, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 14:38:44 -0400 13, 28 -- Received: from d01av04.pok.ibm.com (loopback [127.0.0.1]) 13, 28 -- by d01av04.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id m4EIchTR019202 13, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 14:38:43 -0400 13, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 13, 28 -- by d01av04.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id m4EIchnv019193 13, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 14 May 2008 14:38:43 -0400 13, 28 -- In-Reply-To: {200805131235.m4DCZbfg006596-at-ns.microscopy.com} 13, 28 -- To: Microscopy-at-MSA.Microscopy.Com 13, 28 -- MIME-Version: 1.0 13, 28 -- Subject: Re: [Microscopy] Interference on an SEM 13, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 13, 28 -- Message-ID: {OF20270FF3.E11262AE-ON85257449.0065E561-05257449.006BE28F-at-us.ibm.com} 13, 28 -- From: Darrell Miles {milesd-at-us.ibm.com} 13, 28 -- Date: Wed, 14 May 2008 13:38:42 -0500 13, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 7.0.2FP2 IGS702FP2HF8|March 13, 28 -- 12, 2008) at 05/14/2008 14:38:43, 13, 28 -- Serialize complete at 05/14/2008 14:38:43 13, 28 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
You have clearly carried out some tests for fields but here are more.
1. Long working distance (25-30mm) does the problem become greater? 2. Short working distance (5-7mm) does the problem become less?
Or
3. Low kV (5) does the problem become greater? 4. High kV (30) does the problem become less?
Each of these series proves the field problem if you have a change.
Third test
I have rotated a column 45 degrees, the question being is the problem the same or does it change? A change in physical orientation providing a change in the problem indicates it is external as internal (microscope) faults will stay exactly the same.
Good luck
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {john.mitchels-at-gmail.com} To: {protrain-at-emcourses.com} Sent: Tuesday, May 13, 2008 1:35 PM
Do UPS cause magnetic interference to EM? If so, what solutions for UPS
are in use in EM labs. The question came us as to whether a UPS in rooms adjacent to Ems might interfere with them.
Thanks in advance for any input on this. --aryeh -- Aryeh Weiss School of Engineering Bar Ilan University Ramat Gan 52900 Israel
Ph: 972-3-5317638 FAX: 972-3-7384050
==============================Original Headers============================== 7, 17 -- From aryeh-at-cc.huji.ac.il Thu May 15 03:40:17 2008 7, 17 -- Received: from fortimail.biu.ac.il (fortimail.biu.ac.il [132.70.196.100]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4F8eGfQ028403 7, 17 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2008 03:40:17 -0500 7, 17 -- Received: from [127.0.0.1] ([132.70.133.104]) 7, 17 -- by fortimail.biu.ac.il with ESMTP id m4F9eiKE029947 7, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 15 May 2008 11:40:45 +0200 7, 17 -- Message-ID: {482BF84D.3030209-at-cc.huji.ac.il} 7, 17 -- Date: Thu, 15 May 2008 11:46:05 +0300 7, 17 -- From: Aryeh Weiss {aryeh-at-cc.huji.ac.il} 7, 17 -- Reply-To: aryeh-at-cc.huji.ac.il 7, 17 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 7, 17 -- MIME-Version: 1.0 7, 17 -- To: Microscopy-at-microscopy.com 7, 17 -- Subject: UPS interference to EM 7, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We did some measures on UPS last fall, before the installation of a new TEM, as we took the opportunity to add UPSs too on the SEM and the existing TEM. The question was where localise them, on one hand to answer the same question then you, and on the other hand to avoid long cables between them and the scopes.
The specifications of the manufacturer gives only some indications in the MHz/GHz range, to assure that there are no interferences with phones, wifi etc. For EM, it's more the 0-10 kHz band which is important.
We did the measure with a 100 kHz spectrum analyser and a coil, in a room far from all power lines, at distances from 3 and 6m. The results 6 m away show that compared to the local background (UPS out), if it is powered on without load, frequency between 400 and 1500 Hz increase a bit (from 0.2 to 0.4 mG). With a resistive load of 6 kW, frequency between 1500 and 3000 Hz increase from the same order. The UPS itself has a frequency at 8 kHz of less than 0.05 mG, with it's harmonics at 16, 32, etc kHz.
The conclusion was that if we put each UPS 3-5 m away from it's scope (in the technical room, next to the scope) shure we will be quite. The noise from the power line supplying the scope and its power supply itself wil produce much more interferences. And it's the case. For the SEM, there is a level of less than 0.2 mG near the column with the UPS without load, and it goes up to 0.4 mG when I switch the SEM on !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
aryeh-at-cc.huji.ac.il a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Do UPS cause magnetic interference to EM? If so, what solutions for UPS } } are in use in EM labs. The question came us as to whether a UPS in rooms } adjacent to Ems might interfere with them. } } Thanks in advance for any input on this. } --aryeh }
==============================Original Headers============================== 10, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu May 15 07:06:36 2008 10, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4FC6ZfU020246 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2008 07:06:36 -0500 10, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m4FC6X4S026181 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2008 14:06:33 +0200 (CEST) 10, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 331B8108937F 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Thu, 15 May 2008 14:05:38 +0200 (CEST) 10, 29 -- Message-ID: {482C2712.2040400-at-ipcms.u-strasbg.fr} 10, 29 -- Date: Thu, 15 May 2008 14:05:38 +0200 10, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 29 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080502) 10, 29 -- MIME-Version: 1.0 10, 29 -- To: Microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] UPS interference to EM 10, 29 -- References: {200805150846.m4F8kFqZ004140-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200805150846.m4F8kFqZ004140-at-ns.microscopy.com} 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-IPCMS-MailScanner: Found to be clean 10, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.154]); Thu, 15 May 2008 14:06:33 +0200 (CEST) 10, 29 -- X-Virus-Scanned: ClamAV 0.93/6668/Tue Apr 8 13:57:49 2008 on mr4.u-strasbg.fr 10, 29 -- X-Virus-Status: Clean 10, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=disabled 10, 29 -- version=3.2.4 10, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr4.u-strasbg.fr ==============================End of - Headers==============================
The Electron Microscopy Core at the University of Missouri is hosting the 6th Annual Short Course and Workshop on Computer-Assisted Image Analysis and Measurement taught by Dr. John C. Russ on June 24-27, 2008. This popular course is intended to familiarize users of image analysis hardware and software with the fundamental principles and methods available to obtain meaningful results.
This year¹s workshop will be expanded from 3 to 4 days. The first morning will begin with ³An Introduction to Adobe Photoshop for Scientific Imaging² to serve as a primer for attendees with no experience using Photoshop and as a refresher for those who have not used it on a regular basis. Also, more time will be allotted for the lab exercises and for students to develop imaging strategies while working on their own micrographs.
Image analysis and measurement techniques are utilized in a broad range of applications usually to extract numerical values (number, size, shape, etc.) or the location of objects. In other cases, global structural parameters such as the volume and surface of features are of interest. These measurements may require image processing to correct defects, enhance features, compare multiple images, recognize objects, or other steps. Measurements on individual features, or on the image as a whole, must then be obtained and interpreted in a proper stereological context to obtain useful data. Statistical interpretation of the data allows comparisons of different populations, understanding of distribution plots, and other inferences about the original objects. Structural modeling and geometric probability can be used to develop models for this interpretation.
The course relies heavily on tightly coupled lectures and hands-on exercises covering a wide variety of methodologies, approaches and tools, through a set of practical, step-by-step instructions to minimize the learning curve. No specific background is assumed, although users should already be familiar with microscopy or other imaging technologies. The software platform for the examples and exercises is Adobe Photoshop utilizing a comprehensive set of plug-ins from Reindeer Graphics.
Dr. Russ is the internationally acclaimed author of innumerable articles and several books on image analysis techniques and digital imaging, including the The Image Processing Handbook. He is widely known for his workshops and short courses and has helped to develop software packages to make image analysis methods more accessible to non-specialists. Many of the examples used in the course involve light or electron microscope images, but students are invited to bring their own images (TIFF files) for discussion and analysis. Ample time is provided at the end of the course for individual instruction.
The registration fee is $1400. Enrollment is limited and there are still seats available. More information can be found at: www.emc.missouri.edu/works.htm or by contacting the course coordinator Lou Ross.
Lou Ross Senior Electron Microscope Specialist Electron Microscopy Core Facility W136 Veterinary Medicine University of Missouri Columbia, MO 65211-5120 (573) 882-4777, fax 884=2227 email: rosslm-at-missouri.edu http://www.emc.missouri.edu
==============================Original Headers============================== 4, 22 -- From RossLM-at-missouri.edu Thu May 15 07:15:04 2008 4, 22 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4FCF1VD028679 4, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 15 May 2008 07:15:03 -0500 4, 22 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 4, 22 -- Thu, 15 May 2008 07:15:01 -0500 4, 22 -- Received: from 12.207.81.210 ([12.207.81.210]) by UM-XMAIL06.um.umsystem.edu ([209.106.228.42]) via Exchange Front-End Server webmail.um.umsystem.edu ([209.106.228.51]) with Microsoft Exchange Server HTTP-DAV ; 4, 22 -- Thu, 15 May 2008 12:15:01 +0000 4, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 4, 22 -- Date: Thu, 15 May 2008 07:14:59 -0500 4, 22 -- Subject: 6th Annual Short Course on Computer-Assisted Image Analysis 4, 22 -- From: Lou Ross {rosslm-at-missouri.edu} 4, 22 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 4, 22 -- Message-ID: {C4519373.1006%rosslm-at-missouri.edu} 4, 22 -- Thread-Topic: 6th Annual Short Course on Computer-Assisted Image Analysis 4, 22 -- Thread-Index: Aci2hUtIifuTfCJ4Ed2LcgAewg+K4g== 4, 22 -- Mime-version: 1.0 4, 22 -- Content-type: text/plain; 4, 22 -- charset="ISO-8859-1" 4, 22 -- X-OriginalArrivalTime: 15 May 2008 12:15:01.0195 (UTC) FILETIME=[4C9751B0:01C8B685] 4, 22 -- Content-Transfer-Encoding: 8bit 4, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4FCF1VD028679 ==============================End of - Headers==============================
The College of Microscopy, located in Westmont, IL, is offering a short course in Polarized Light Microscopy June 16-20, 2008.
A Microscopy Camp will be held June 23-27, 2008, and is intended for high school and middle school teachers. Each teacher will receive a Motic microscope, digital camera and software to take back to their classroom.
For further details and registration information for the PLM course or for Microscopy Camp, please follow the link below:
www.collegeofmicroscopy.com
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
Aryeh; It depends on the particular make and model of the UPS. For the Phase One (Now Danaher) UPS for our 2010F, JEOL recommends over 30 feet distance from the UPS to the column, and that is for a 200KV microscope!
John Mardinly, Numonyx
-----Original Message----- X-from: aryeh-at-cc.huji.ac.il [mailto:aryeh-at-cc.huji.ac.il] Sent: Thursday, May 15, 2008 1:49 AM To: MARDINLY, A
Do UPS cause magnetic interference to EM? If so, what solutions for UPS
are in use in EM labs. The question came us as to whether a UPS in rooms adjacent to Ems might interfere with them.
Thanks in advance for any input on this. --aryeh -- Aryeh Weiss School of Engineering Bar Ilan University Ramat Gan 52900 Israel
Ph: 972-3-5317638 FAX: 972-3-7384050
==============================Original Headers============================== 7, 17 -- From aryeh-at-cc.huji.ac.il Thu May 15 03:40:17 2008 7, 17 -- Received: from fortimail.biu.ac.il (fortimail.biu.ac.il [132.70.196.100]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4F8eGfQ028403 7, 17 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2008 03:40:17 -0500 7, 17 -- Received: from [127.0.0.1] ([132.70.133.104]) 7, 17 -- by fortimail.biu.ac.il with ESMTP id m4F9eiKE029947 7, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 15 May 2008 11:40:45 +0200 7, 17 -- Message-ID: {482BF84D.3030209-at-cc.huji.ac.il} 7, 17 -- Date: Thu, 15 May 2008 11:46:05 +0300 7, 17 -- From: Aryeh Weiss {aryeh-at-cc.huji.ac.il} 7, 17 -- Reply-To: aryeh-at-cc.huji.ac.il 7, 17 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 7, 17 -- MIME-Version: 1.0 7, 17 -- To: Microscopy-at-microscopy.com 7, 17 -- Subject: UPS interference to EM 7, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From A.MARDINLY-at-numonyx.com Thu May 15 12:23:32 2008 16, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4FHNVQT013555 16, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 15 May 2008 12:23:31 -0500 16, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 16, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 08D324141C9; 16, 29 -- Thu, 15 May 2008 14:00:55 -0400 (EDT) 16, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 16, 29 -- Thu, 15 May 2008 13:23:30 -0400 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 29 -- Content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="us-ascii" 16, 29 -- Subject: RE: [Microscopy] UPS interference to EM 16, 29 -- Date: Thu, 15 May 2008 13:23:18 -0400 16, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF939A645-at-EXDRESBENMX012.numonyx.local} 16, 29 -- In-Reply-To: {200805150849.m4F8nFbH008701-at-ns.microscopy.com} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] UPS interference to EM 16, 29 -- Thread-Index: Aci2aKKD7XesZ+wLQ/qog+QvGGQt5gAR1+/A 16, 29 -- References: {200805150849.m4F8nFbH008701-at-ns.microscopy.com} 16, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 16, 29 -- To: {aryeh-at-cc.huji.ac.il} 16, 29 -- Cc: {Microscopy-at-MSA.Microscopy.Com} 16, 29 -- X-OriginalArrivalTime: 15 May 2008 17:23:30.0922 (UTC) FILETIME=[653F84A0:01C8B6B0] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4FHNVQT013555 ==============================End of - Headers==============================
We use two of them in our microscopy fscility. john
At 01:12 PM 5/15/2008, gmartens-at-interchange.ubc.ca wrote:
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Email: duleyml-at-muohio.edu Name: Matt Duley
Organization: Miami University
Title-Subject: [Filtered] "Pure" water??
Question: Good afternoon all,
We are a multiuser EM and Light microscopy facility. We are going to be moving our facility across campus in the next year (or so) and will be losing easy access to the Nanopure water system we have been using. We use the Nanopure water for rinses etc. The water here in Oxford is not good, we get contaminants even with the Nanopure system that show up at the TEM level. We have begun using ìmolecular gradeî water from Fischer Scientific for our fixatives and stains, UA, lead citrate etc. When we made the switch to the molecular grade water for our fixatives and stains most of the problems went away. Obviously, that stuff is too expensive to use for rinses and general use. My question is this, would it be worth our while to pursue a glass still, (there is a ìbrokenî one in another dept., heating element I think) to take with us when we move, or do we get another Nanopure system? What are your labs doing for ìpureî water?
Your thoughts?
Matthew L. Duley
Electron Microscopist Electron Microscopy Facility 359 Pearson Hall Miami University Oxford, Ohio 45056 513.529.4164 FAX 513.529.4243 Duleyml-at-muohio.edu
-----Original Message----- X-from: spamMfilter-at-microscopy.com [mailto:spamMfilter-at-microscopy.com] Sent: Thursday, May 15, 2008 4:26 PM To: Barbara Plowman
I'm sure there are several solutions to obtaining pure water.
Here is the sequence of purification that we currently use: tap water is run through a string filter to remove particulates, a carbon filter to remove organics, two deionizers (high capacity followed by high purity), finally glass distillation.
In another research lab (earlier in my career), we used two glass stills: one high capacity still to produce the initial product that was stored in a 15 gallon glass reservoir and a smaller still that was connected to the 15 gallon tank to redistill it. This worked fairly well.
You should start with tap water, rather than "house distilled" water that may available from a spigot in your building. These "in house" units sometimes condense steam from boilers that are used to heat the building. Unfortunately, volatile amines are added to the steam to prevent corrosion or clogging of pipes and, guess what, they distill over and end up causing precipitation problems with your EM reagents.
JB
} } } Email: duleyml-at-muohio.edu } Name: Matt Duley } } We are a multiuser EM and Light microscopy } facility. We are going to be moving our facility } across campus in the next year (or so) and will } be losing easy access to the Nanopure water } system we have been using. We use the Nanopure } water for rinses etc. The water here in Oxford } is not good, we get contaminants even with the } Nanopure system that show up at the TEM level. } We have begun using Ïmolecular gradeÓ water from } Fischer Scientific for our fixatives and stains, } UA, lead citrate etc. When we made the switch to } the molecular grade water for our fixatives and } stains most of the problems went away. } Obviously, that stuff is too expensive to use for } rinses and general use. My question is this, } would it be worth our while to pursue a glass } still, (there is a ÏbrokenÓ one in another dept., } heating element I think) to take with us when we } move, or do we get another Nanopure system? What } are your labs doing for ÏpureÓ water?
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: Bplowman-at-pacific.edu Name: Barbara Plowman
Organization: UOP Arthur A Dugoni School of Dentistry
Title-Subject: [Filtered] vacuum cleaner
Question: Hi! I got a great little commercial Orek (used) on EBay for $89. They have all sorts of used Oreks. Just read carefully and take your pick!
My view is that fixatives aren't good for long term wet storage - for formalin (e.g. 10% formaldehyde) fixed tissue supplied by colleagues, I generally transferred the tissue to a 70 to 75% ethanol for long term storage - and that worked fine (they were often for autoradiograph and simple H&E production). Unless tiddly mouse organs, I sliced larger tissue though - I guess I just like every looking nice. I don't have my original data that lead me to do all this, but an internet search came up the following that agrees with my recollections: Formalin 'makes a very poor long-term preservative for many specimens, resulting in the decalcification of bones, distortion of tissues, and acidic decomposition of specimens.. Yes, formic acid is created when formaldehyde reacts with oxygen. Long Term storage in formic acid, even low levels, will cause destruction of Proteins in the tissue."
Plus "Additional cross-links are formed, which can interfere with staining, by blocking epitopes or distorting the proteins so they are not stainable. A review of the Internet shows that the Australian Museum Fish site states they have fixed in "formaldehyde and then stored in alcohol for the last 80 years". See links below.
Why would you risk further structural tissue damage by freezing, once you have used liquid fixatives etc.. anyway? - unless you had a very good reason, and granted many do. [I have used untreated pre-chilled [4oC] tissue blocks frozen into the cryo-wax block at [-25oC] on the cryostat and then sectioned/archived in a -20oC freezer - or I have snap frozen in Al foil boats floating on super-cooled liquids - all with no fixatives/protectants prior to freezing - I fix the cut section]. Snap freezing in super-cool liquids supposedly produces the better histology, i.e. less ice crystal damage. A lot suggest isopentane at -160oC is better than liquid nitrogen, but I stuck to my floating foil boats [they are kinda fun], making sure no liquid ever contacts the wet specimen until fully frozen - the tissue freezes up from the cold dry foil surface. Any N2 would boil off otherwise when it contacts the 'warm' specimen and freeze it too slowly. Never tried large organs though (you are going to need a bigger boat! - probably a block of steel with it's base in the super-coolant). You can fix the cryostat tissue section (alcohol) after it's cut or liquid fix before if that's what they are doing, and use cryo-protectant (e.g. sucrose), why not.
I only freeze tissue though when I wish to avoid liquid fixatives or need things 'frozen at that moment' - i.e. when I really have to. I've never used any cryo-protectant or room temperature liquids on the tissue before freezing [though many have]. Fixed tissue will still need to be snap frozen to minimise ice crystal damage, but I would use a cryo-protectant (e.g. sucrose). I would think it would be more difficult to snap freeze a very large organ without ice crystal damage, although the fixative and cryo-protectant may well make this easier - pre-cool to 4oC and immersion in isopentane has been suggested. Fixed tissue is supposed to look a tad better when cryostat sectioned (never tried it, but see last link), but then fixed tissue and standard room-temp histology looks even better than any frozen sections.
For one large study I rapid liquid nitrogen froze tissue (murine testis) that had soluble Pu-239 within it. As these were for autoradiograph production, absolutely no liquids could be used as it could relocate the internal solubilised Pu. So no cryo-protectant or suchlike, the whole fresh organs [and associated tissue] were snap frozen on the foil boats (and then stored under liquid nitrogen with one small tissue bit per cryo-tube). Over the next month, frozen cryostat section autoradiographs were prepared using K5 liquid [film] emulsion coated slides or similar, such that the tissue was never de-frosted until autoradiograph [film] development. Section quality ranged from poor to appalling, but it was those little Pu-239 alpha-particle tracks we were interested in. It never crossed my mind to cryo-freeze histological tissue that might go on for further standard liquid processing, i.e. just for archiving tissue. Although Liquid Nitrogen is naturally standard for preserving/archiving live cell lines, bacteria etc.. that might need re-culturing again (and wouldn't take kindly to fixatives). The pathologyinnovations site link below is pretty good for cryostat/frozen tissue sectioning advice.
If your sample is already in liquid fixative nominally at room temperature then I would leave it that way (i.e. transfer for long term storage into a liquid such as 70% ethanol, or cacodylate buffer if fixed by gluteraldehyde, and on into a 4oC fridge). I always stored in a fridge to eliminate any unpleasantness [it's very cold and dark] and it will minimise evaporation.
As I mentioned previously though formalin definitely wasn't my preferred fixative, I used osmium tetroxide/Freon, gluteraldehyde secondary fixative and long term storage in cacodylate buffer for 5 to 10 years (in the fridge) with unblocked samples still OK for good TEM sections many years later. My tissue was mostly rodent lung and always sliced into 'slices' of 3 to 5 mm, one slice per small storage vial (screw top Packard plastic 10ml liquid scintillation vials from memory) - certainly not whole organs. As a new post-doc I was given some lung slices in gluteraldehyde fixative and I assumed they were in cacodylate buffer. Off to our histology and six months later the sections were so awful that we had to repeat the time-point again for new lung tissue (and with long-term storage in cacodylate buffer all was fine). So I don't like storing in fixative - even formalin.
So my vote is ask - why freeze over continued liquid storage exactly, when your sample is in RT liquid formalin already? Are you sectioning later with a cryostat or conventional histology? There can be good reasons to combine liquid fix/standard processing and freezing (see 'uptight molluscs'). If you are using conventional room temperature histology post freezing, then think: why freeze?
Well that’s my opinion. I like questions like this on the list-server, it reminds me why I do things [i.e. it' not all just habit and 'well it mostly works OK']. However freezing tissue samples for cryostat sectioning/histology is one of those really critical operations, hence there's certainly a lot of options, opinions and methods [tried, theorized and discussed] out there.
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
http://www.histosearch.com/histonet/Sep04A/Re.HistonetLongtermstoragB.html http://www.nps.gov/history/museum/publications/conserveogram/11-01.pdf http://mollus.oxfordjournals.org/cgi/reprint/62/1/124.pdf relaxing the uptight molluscs
http://www.pathologyinnovations.com/new_page_3.htm the awful frozen section, and how to avoid it. You can get good frozen sections, e.g. if you fix the cryo-section using liquid fixatives (ethanol).
http://www.histosearch.com/histonet/Nov05A/Re.HistonetamIsnap-freezi.html freezing with isopentane chat
http://www.biotech.ufl.edu/EM/data/freeze.html freezing frozen section with cryoprotectant
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 14 May 2008 11:10 To: kjmorris-at-well.ox.ac.uk
Dear all, I have been asked to found the best method to freeze entire rat organs for an undetermined period of time, maybe years. They may be recovered any time for histology observation. I told the people that it was probably better to leave them in a fixative at 4°C but they want them frozen. I am not very experienced in cryopreservation (except for cells in culture), especially of whole organs. For me the ideal way would be to perfuse with glycerol (I seem to remember something like 60%) but I simply wonder if we master the technique to cryopreserve whole rat organs. Fact is, here we need only to maintain the structure, not the activity! Problem is, the organs cannot be perfused. They will be fixed in formalin and given to me whole. If anyone has experience or infos to share, they are welcome. Stephane
P.S: don't laugh: they just wanted to quick-freeze them by directly plunging the organs in liquid nitrogen!
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==============================Original Headers============================== 32, 23 -- From kjmorris-at-well.ox.ac.uk Fri May 16 04:05:27 2008 32, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 32, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4G95Q6F009239 32, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 May 2008 04:05:27 -0500 32, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 32, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 32, 23 -- id 1JwvsU-0003xn-8T 32, 23 -- for Microscopy-at-Microscopy.Com; Fri, 16 May 2008 10:05:26 +0100 32, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 32, 23 -- To: {Microscopy-at-Microscopy.Com} 32, 23 -- References: {200805141010.m4EAALlV025657-at-ns.microscopy.com} 32, 23 -- Subject: =?iso-8859-1?Q?RE:_=5BMicroscopy=5D_Storage_of_organs_at_-=B0C?= 32, 23 -- Date: Fri, 16 May 2008 10:07:23 +0100 32, 23 -- Message-ID: {000001c8b734$417b4840$b22c4381-at-CytoWhizz} 32, 23 -- MIME-Version: 1.0 32, 23 -- Content-Type: text/plain; 32, 23 -- charset="iso-8859-1" 32, 23 -- X-Mailer: Microsoft Office Outlook 11 32, 23 -- In-Reply-To: {200805141010.m4EAALlV025657-at-ns.microscopy.com} 32, 23 -- Thread-Index: Aci1qrlsATwkEeRpS+uveuCQCZvx0AAD6KYg 32, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 32, 23 -- Content-Transfer-Encoding: 8bit 32, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4G95Q6F009239 ==============================End of - Headers==============================
Hello: We have started looking at the 3D software that is out for SEM's. I assume there are some of you who have been through this and was hoping for some comments. Are there some preferred systems, how useful have you found these to be once you started using them, what limitations turned up. Thanks for any help and please contact me off the list serve if you think appropriate. Steve
Stephen McCartney Senior Research Associate Nanoscale Characterization and Fabrication Laboratory (NCFL) Institute for Critical Technology and Applied Science (ICTAS) 1991 Kraft Drive Va Tech Blacksburg, VA 24061 540-231-9765
==============================Original Headers============================== 3, 20 -- From stmccart-at-vt.edu Fri May 16 07:45:14 2008 3, 20 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GCjEri032616 3, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 07:45:14 -0500 3, 20 -- Received: from freya.cc.vt.edu (IDENT:mirapoint-at-[10.1.1.15]) 3, 20 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id m4GCjDHG026748 3, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 08:45:13 -0400 3, 20 -- Received: from mri-system.vt.edu ([198.82.148.26]) 3, 20 -- by freya.cc.vt.edu (MOS 3.8.6-GA) 3, 20 -- with ESMTP id AOE76448; 3, 20 -- Fri, 16 May 2008 08:45:13 -0400 (EDT) 3, 20 -- Message-Id: {5.2.1.1.0.20080516084025.0145dbb8-at-pop.vt.edu} 3, 20 -- X-Sender: stmccart-at-pop.vt.edu 3, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 3, 20 -- Date: Fri, 16 May 2008 08:45:12 -0400 3, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 20 -- From: Stephen McCartney {stmccart-at-vt.edu} 3, 20 -- Subject: SEM 3D software 3, 20 -- Mime-Version: 1.0 3, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Email: s.miao-at-sheffield.ac.uk Name: Shu Miao
Title-Subject: [Filtered] Diamond wire saw
Question: Hello all, I'm looking for advice to buy a diamond wire saw. We will mostly to use it cut cross-section samples. Currently, we are looking at the WELL 3032 and South Bay Technology 810. I had pleasant experience with 3032 before. But the 810 also looks good. What I worry is the 810 holds the sample facedown. Will a small pallet comes off from the holder when cutting a cross-section stack? I encountered this problem when using a verticle saw like WELL 3242. Does anyone have recommendation on other brands? Thanks
Shu Miao Department of Engineering Materials University of Sheffield
I'm a big fan of the Nilfisk industrial vacuums, especially since some of them are designed specifically for hazardous products. The GM 80 with variable speed control has been extremely useful, and it has a true HEPA exhaust filter as well as an internal dust collection bag. They're not cheap, but they last literally forever.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635 Fax: +1 (931) 685-6613 robert.zonis-at-sanford.com www.papermate.com
-----Original Message----- X-from: gmartens-at-interchange.ubc.ca [mailto:gmartens-at-interchange.ubc.ca] Sent: Thursday, May 15, 2008 3:14 PM To: Zonis, Robert
Hi
We are in the market for a good vacuum cleaner to clean around our microscopes and other equipment. Any recommendations?
Garnet
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==============================Original Headers============================== 19, 33 -- From Robert.Zonis-at-Sanford.com Fri May 16 08:11:10 2008 19, 33 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.245.99]) 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4GDB9Jf020930 19, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 16 May 2008 08:11:10 -0500 19, 33 -- X-VirusChecked: Checked 19, 33 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 19, 33 -- X-Msg-Ref: server-9.tower-192.messagelabs.com!1210943467!1009025!13 19, 33 -- X-StarScan-Version: 5.5.12.14.2; banners=sanford.com,-,- 19, 33 -- X-Originating-IP: [198.176.16.26] 19, 33 -- Received: (qmail 12004 invoked from network); 16 May 2008 13:11:10 -0000 19, 33 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) (198.176.16.26) 19, 33 -- by server-9.tower-192.messagelabs.com with SMTP; 16 May 2008 13:11:10 -0000 19, 33 -- Received: from nashbsasebe01.nr.ad.newellco.com ([10.217.223.198]) by naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 33 -- Fri, 16 May 2008 08:11:04 -0500 19, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 33 -- Content-class: urn:content-classes:message 19, 33 -- MIME-Version: 1.0 19, 33 -- Content-Type: text/plain; 19, 33 -- charset="us-ascii" 19, 33 -- Subject: RE: [Microscopy] vacuum cleaner 19, 33 -- Date: Fri, 16 May 2008 08:10:37 -0500 19, 33 -- Message-ID: {8809C39726DC5F43824A3D3BE5D0286A0EF906D6-at-nashbsasebe01.nr.ad.newellco.com} 19, 33 -- X-MS-Has-Attach: 19, 33 -- X-MS-TNEF-Correlator: 19, 33 -- Thread-Topic: [Microscopy] vacuum cleaner 19, 33 -- Thread-Index: Aci2yCoNFI6+Uso6Tw6oonp/R/femQAjBv6w 19, 33 -- References: {200805152013.m4FKDZON009742-at-ns.microscopy.com} 19, 33 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 19, 33 -- To: {gmartens-at-interchange.ubc.ca} 19, 33 -- Cc: {Microscopy-at-microscopy.com} 19, 33 -- X-OriginalArrivalTime: 16 May 2008 13:11:04.0876 (UTC) FILETIME=[4BEA0EC0:01C8B756] 19, 33 -- Content-Transfer-Encoding: 8bit 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4GDB9Jf020930 ==============================End of - Headers==============================
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I don't have good experience with both of the wire saws which you have mentioned ( South bay and Well3032). After made a long search finally end up in buying wire saw (WS22) which was manufactured in Poland, you need to have to find the distributor in UK. I am pretty happy with the cutting but only the problem wire tension may not stay for long, so needs to adjusted time to time. You can find more information about this wire saw by the following link
http://www.princetonscientific.com/pages/WIRE.PDF
The price is also pretty reasonable and one can use Boron carbide other as a cutting grid. It takes long time for cutting ceramic materials, other than that everything is fine.
-------------- Rao Karavadi Research Associate Center for Advanced Materials & Nanotechnology Lehigh University Bethlehem, PA 18015 USA
I just use a very cheap (£45) cylinder vacuum cleaner (see link). You always need the upholstery brush and the narrow nozzle (and never need the tubes, carpet head). Variable speed suction is very useful to save sucking up glass slides. Just keep away from the optics (or whatever they are called on an EM). Most important are disposable dust bags and full HEPA filtration (get spare bags/HEPA filters sets when ordering) - don't use bagless cleaners as too much dust is released when emptying the container (even if they have HEPA filters) - OK at home when emptying outdoors but awful with the lab bin. A flat rear really helps as the cleaner can sit on it and the hose goes vertically up towards the microscope (and it can wheel about laid back down again). The cleaner lasts forever as it's only used once a month or so for deep fluff (I'm not house proud).
Apparently all VAX cyclinders have HEPA filtration. I'm not advocating VAX particularly, just the HEPA spec, bag and variable suction (and low price). I'm sure say a Miele does it with more style (and cost). My previous one at UCL was Morphy Richards, but it was as cheap, small and looked/worked identical to the VAX. Don't need massive suction so 1800watts is fine/overkill, and small is beautiful.
Also use it to clean out dust bunnies from the microscope PCs (don't touch the circuit board though as it finds the static generated disagreeable). Dust in PCs leads to overheating and failure. Use an air jet to blow dust from the power supply enclosure and motherboard, heatsinks, memory, fans etc, with the vacuum cleaner hose nearby (and cough/sneeze a lot). Our confocal engineer often borrows the vacuum cleaner to clean out the laser control box hardware as well. Downside, make sure no-one else can borrow it, my UCL one was used by contractors and lab staff for goodness knows what, just because it was lying about and easy to nick.
It can be a fight to get permission to buy one - say it's only for the microscopes and you aren't doing the cleaners job (I don't know if anyone does that these days). Dust is the enemy of the microscopist (and gaming PCs, Lungs etc...).
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: Robert.Zonis-at-Sanford.com [mailto:Robert.Zonis-at-Sanford.com] Sent: 16 May 2008 14:17 To: kjmorris-at-well.ox.ac.uk
Garnet,
I'm a big fan of the Nilfisk industrial vacuums, especially since some of them are designed specifically for hazardous products. The GM 80 with variable speed control has been extremely useful, and it has a true HEPA exhaust filter as well as an internal dust collection bag. They're not cheap, but they last literally forever.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635 Fax: +1 (931) 685-6613 robert.zonis-at-sanford.com www.papermate.com
-----Original Message----- X-from: gmartens-at-interchange.ubc.ca [mailto:gmartens-at-interchange.ubc.ca] Sent: Thursday, May 15, 2008 3:14 PM To: Zonis, Robert
Hi
We are in the market for a good vacuum cleaner to clean around our microscopes and other equipment. Any recommendations?
Garnet
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==============================Original Headers============================== 19, 33 -- From Robert.Zonis-at-Sanford.com Fri May 16 08:11:10 2008 19, 33 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.245.99]) 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4GDB9Jf020930 19, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 16 May 2008 08:11:10 -0500 19, 33 -- X-VirusChecked: Checked 19, 33 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 19, 33 -- X-Msg-Ref: server-9.tower-192.messagelabs.com!1210943467!1009025!13 19, 33 -- X-StarScan-Version: 5.5.12.14.2; banners=sanford.com,-,- 19, 33 -- X-Originating-IP: [198.176.16.26] 19, 33 -- Received: (qmail 12004 invoked from network); 16 May 2008 13:11:10 -0000 19, 33 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) (198.176.16.26) 19, 33 -- by server-9.tower-192.messagelabs.com with SMTP; 16 May 2008 13:11:10 -0000 19, 33 -- Received: from nashbsasebe01.nr.ad.newellco.com ([10.217.223.198]) by naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 33 -- Fri, 16 May 2008 08:11:04 -0500 19, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 33 -- Content-class: urn:content-classes:message 19, 33 -- MIME-Version: 1.0 19, 33 -- Content-Type: text/plain; 19, 33 -- charset="us-ascii" 19, 33 -- Subject: RE: [Microscopy] vacuum cleaner 19, 33 -- Date: Fri, 16 May 2008 08:10:37 -0500 19, 33 -- Message-ID: {8809C39726DC5F43824A3D3BE5D0286A0EF906D6-at-nashbsasebe01.nr.ad.newellco.com} 19, 33 -- X-MS-Has-Attach: 19, 33 -- X-MS-TNEF-Correlator: 19, 33 -- Thread-Topic: [Microscopy] vacuum cleaner 19, 33 -- Thread-Index: Aci2yCoNFI6+Uso6Tw6oonp/R/femQAjBv6w 19, 33 -- References: {200805152013.m4FKDZON009742-at-ns.microscopy.com} 19, 33 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 19, 33 -- To: {gmartens-at-interchange.ubc.ca} 19, 33 -- Cc: {Microscopy-at-microscopy.com} 19, 33 -- X-OriginalArrivalTime: 16 May 2008 13:11:04.0876 (UTC) FILETIME=[4BEA0EC0:01C8B756] 19, 33 -- Content-Transfer-Encoding: 8bit 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4GDB9Jf020930 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 23 -- From kjmorris-at-well.ox.ac.uk Fri May 16 10:21:29 2008 35, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 35, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GFLR1R026840 35, 23 -- for {Microscopy-at-Microscopy.Com} ; Fri, 16 May 2008 10:21:28 -0500 35, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 35, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 35, 23 -- id 1Jx1OW-00073K-Rb 35, 23 -- for Microscopy-at-Microscopy.Com; Fri, 16 May 2008 15:58:52 +0100 35, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 35, 23 -- To: {Microscopy-at-Microscopy.Com} 35, 23 -- References: {200805161317.m4GDHPGU005213-at-ns.microscopy.com} 35, 23 -- Subject: RE: [Microscopy] RE: vacuum cleaner 35, 23 -- Date: Fri, 16 May 2008 16:00:49 +0100 35, 23 -- Message-ID: {000001c8b765$a1026dd0$b22c4381-at-CytoWhizz} 35, 23 -- MIME-Version: 1.0 35, 23 -- Content-Type: text/plain; 35, 23 -- charset="iso-8859-1" 35, 23 -- X-Mailer: Microsoft Office Outlook 11 35, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 35, 23 -- Thread-Index: Aci3VzBGVhgrnwyjTiCIFvALe8u3sQAATNQg 35, 23 -- In-Reply-To: {200805161317.m4GDHPGU005213-at-ns.microscopy.com} 35, 23 -- Content-Transfer-Encoding: 8bit 35, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4GFLR1R026840 ==============================End of - Headers==============================
You indicate that the problem only started with this detector. I would suspect the detector, but I don't think I would suspect the magnet.
The magnet (or the detector in general) should be expected to introduce aberrations as you bring the detector closer to the beam. You need to readjust focus and stigmation as you move the detector. Hopefully, you have plenty of room for adjustment.
If you think the problem is with the magnet, you should be able to temporarily remove the collimator which holds the magnet and check for the problem. I have only seen a few EDS detectors, but for all I have seen the collimator simply slides over the end of the detector and friction holds it in place. You should be able to remove it with the detector still on the SEM. Of course, you need to BE CAREFUL working around the thin window in the end of the detector. ANY direct contact with the window will almost certainly break it.
If the problem remains with the collimator/magnet removed, then I would suspect something else about the detector. Perhaps there is a stray electrical field that is causing the problem. You may be able to check that by powering off your EDS system and disconnecting all electrical connections from the detector.
If neither of those simple tests takes care of the problem, then you do have a challenging issue.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: ederoever-at-nalco.com [mailto:ederoever-at-nalco.com] Sent: Wednesday, May 14, 2008 8:22 AM To: wesaia-at-iastate.edu
COMMERCIAL REPLY: Smart Imaging Technologies has 3D image analysis software that not only does 3D object rendering but offers capability for fully automated metrology and reporting. This solution was selected by Advanced Imaging as 2008 Software Solution of the Year and by R&D100 in 2007 for most innovative technology.
For more information, please visit http://smartimtech.com/3d_analysis.htm or contact us directly.
Ira Bleiweiss Smart Imaging Technologies 713.589.3216 direct 877.280.1100 ext.1004 Toll Free (US) ira-at-simagis.us www.smartimtech.com
Advanced Software Solutions for Science and Industry
-----Original Message----- X-from: stmccart-at-vt.edu [mailto:stmccart-at-vt.edu] Sent: Friday, May 16, 2008 7:56 AM
I would be very cautious operating the microscope and using the EDX detector close to the sample without the collimator, as you could damage the detector. The magnets are there for a purpose: keeping backscattered electrons from hitting the detector. If you try this experiment, keep the accelerating voltage as low as possible. Of course you won't get a lot of X-rays either, but you will be able to verify whether the collimator is causing your problem. If the answer is yes, try to get a replacement detector from Oxford. There is a chance that one of the magnets is in backwards, which could cause your problem.
John Mardinly, Numonyx
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Friday, May 16, 2008 8:33 AM To: MARDINLY, A
You indicate that the problem only started with this detector. I would suspect the detector, but I don't think I would suspect the magnet.
The magnet (or the detector in general) should be expected to introduce aberrations as you bring the detector closer to the beam. You need to readjust focus and stigmation as you move the detector. Hopefully, you have plenty of room for adjustment.
If you think the problem is with the magnet, you should be able to temporarily remove the collimator which holds the magnet and check for the problem. I have only seen a few EDS detectors, but for all I have seen the collimator simply slides over the end of the detector and friction holds it in place. You should be able to remove it with the detector still on the SEM. Of course, you need to BE CAREFUL working around the thin window in the end of the detector. ANY direct contact with the window will almost certainly break it.
If the problem remains with the collimator/magnet removed, then I would suspect something else about the detector. Perhaps there is a stray electrical field that is causing the problem. You may be able to check that by powering off your EDS system and disconnecting all electrical connections from the detector.
If neither of those simple tests takes care of the problem, then you do have a challenging issue.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: ederoever-at-nalco.com [mailto:ederoever-at-nalco.com] Sent: Wednesday, May 14, 2008 8:22 AM To: wesaia-at-iastate.edu
You would have to shave slices off the surface, image the new surface and then input the images into SIMAGIS. So long as the images can be indexed, they may be stacked.
Regards, Ira
-----Original Message----- X-from: Bill Miller [mailto:microbill-at-mohawk.net] Sent: Friday, May 16, 2008 11:46 AM To: ira-at-simagis.us
==============================Original Headers============================== 11, 22 -- From ira-at-simagis.us Fri May 16 11:59:24 2008 11, 22 -- Received: from smtp137.sat.emailsrvr.com (smtp137.sat.emailsrvr.com [66.216.121.137]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GGxOn0018550 11, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 May 2008 11:59:24 -0500 11, 22 -- Received: from relay3.relay.sat.mlsrvr.com (localhost [127.0.0.1]) 11, 22 -- by relay3.relay.sat.mlsrvr.com (SMTP Server) with ESMTP id 2108420CD8B; 11, 22 -- Fri, 16 May 2008 12:59:24 -0400 (EDT) 11, 22 -- Received: by relay3.relay.sat.mlsrvr.com (Authenticated sender: ira-AT-simagis.us) with ESMTP id 0C91920CD2C 11, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 16 May 2008 12:59:24 -0400 (EDT) 11, 22 -- From: "Ira Bleiweiss" {ira-at-simagis.us} 11, 22 -- To: {Microscopy-at-microscopy.com} 11, 22 -- Subject: RE: [Microscopy] RE: SEM 3D software--Commercial Reply 11, 22 -- Date: Fri, 16 May 2008 11:59:17 -0500 11, 22 -- Message-ID: {015e01c8b776$2dabb1f0$04010a0a-at-iblaptop} 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-Type: text/plain; 11, 22 -- charset="us-ascii" 11, 22 -- Content-Transfer-Encoding: 7bit 11, 22 -- X-Mailer: Microsoft Office Outlook 11 11, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 11, 22 -- thread-index: Aci3dFry9V6fnOoAQTaDVkilaLGbhwAAHafA 11, 22 -- In-Reply-To: {0K0Y00GXCZWZ7QD0-at-mta2.srv.hcvlny.cv.net} ==============================End of - Headers==============================
Dear colleagues Currently, we have an open postdoctoral position in the fields of nanoscale electromechanics, piezoresponse force microscopy, and band-excitation SPM. The full announcement is listed below, and can also be found on http://orise.orau.gov/sep/needs/files/ORNL08-79-CNMS.pdf
Please feel free to distribute this announcements further to your colleagues, and contact me for additional details. Yours Sergei V. Kalinin
Postdoctoral Research Associate in Scanning Probe Microscopy for Functional Imaging Center for Nanophase Materials Sciences Oak Ridge National Laboratory Oak Ridge, Tennessee ORNL08-79-CNMS Project Description:
The Center for Nanophase Materials Science (CNMS) at Oak Ridge National Laboratory (ORNL) is seeking a candidate to fill a postdoctoral position in the field of scanning probe microscopy. This program takes advantage of recently developed capabilities for Piezoresponse Force Microscopy (PFM) and a family of Band Excitation Scanning Probe Microscopies, including new spectroscopic modes for studies of polarization dynamics and energy transfer in a wide range of materials, including oxides and polymers. The CNMS (http://cnms.ornl.qov/) is a collaborative nanoscience user research facility established by the Office of Science, U.S. Department of Energy. The CNMS has a diverse spectrum of nanoscience research activities including emphasis on synthesis and characterization of complex oxides, polymers, catalysts, and nanostructures. The Scanning Probe group has six microscopes with capabilities developed at ORNL for imaging functional properties.
Qualifications: This position requires a Ph.D. in the physical sciences, with an emphasis on atomic force microscopy. The successful applicant must demonstrate experience in the experimental application, development, and quantitative interpretation of modern scanning probe techniques. A working knowledge of MatLab is an advantage. This position provides an opportunity to join an experienced team to develop methods and applications in directions such as identification and control of static and dynamic ferroelectric phenomena at the nanometer scale, electromechanical probing on the single-molecule level, and energy or time-resolved measurements. Excellent oral and written communication skills in English are required. Presentations to an international scientific audience and publication of scientific results in peer-reviewed journals are required. . The applicant must have the ability to work in a team and interact effectively with a broad range of colleagues. In addition, the candidate will be involved in the CNMS user program, with wide opportunities for scientific collaborations. All degree requirements must be completed before starting the appointment.
How to Apply: Qualified applicants must apply online at https://www2.orau.gov/ORNL_POST/. All applicants will need to register before they can begin the online application. For complete instructions, on how to apply, please see the instructions at http://www.orau.gov/orise/edu/ornl/ornl-pdpm/application.htm. When applying for this position, please reference the position title and number.
Questions regarding the position can be directed to Dr. Sergei V. Kalinin, (sergei2-at-ornl.gov). Applications will be accepted until October 1, 2008 or until the position is filled.
This appointment is offered through the ORNL Postgraduate Research Participation Program and is administered by the Oak Ridge Institute for Science and Education (ORISE). The program is open to all qualified U.S. and non-U.S. citizens without regard to race, color, age, religion, sex, national origin, physical or mental disability, or status as a Vietnam-era veteran or disabled veteran.
==============================Original Headers============================== 10, 25 -- From sergei2-at-ornl.gov Fri May 16 12:44:42 2008 10, 25 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GHigZd001053 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 12:44:42 -0500 10, 25 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 10, 25 -- by emroute3.ornl.gov (PMDF V6.3-x14 #31501) 10, 25 -- with ESMTP id {0K0Z00E1L2MH4D-at-emroute3.ornl.gov} for 10, 25 -- microscopy-at-microscopy.com; Fri, 16 May 2008 13:44:41 -0400 (EDT) 10, 25 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 10, 25 -- (PMDF V6.3-x14 #31501) id {0K0Z00E012MH7A-at-emroute3.ornl.gov} for 10, 25 -- microscopy-at-microscopy.com; Fri, 16 May 2008 13:44:41 -0400 (EDT) 10, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 10, 25 -- by emroute3.ornl.gov (PMDF V6.3-x14 #31501) 10, 25 -- with ESMTP id {0K0Z00CBQ2MHXZ-at-emroute3.ornl.gov} for 10, 25 -- microscopy-at-microscopy.com; Fri, 16 May 2008 13:44:41 -0400 (EDT) 10, 25 -- Date: Fri, 16 May 2008 13:44:41 -0400 10, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 10, 25 -- Subject: Postdoctoral Position - Band Excitation SPM and Piezoresponse Force 10, 25 -- Microscopy 10, 25 -- To: microscopy-at-microscopy.com 10, 25 -- Message-id: {482DC809.1020805-at-ornl.gov} 10, 25 -- MIME-version: 1.0 10, 25 -- Content-type: text/plain; format=flowed; charset=windows-1252 10, 25 -- Content-transfer-encoding: 7bit 10, 25 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) ==============================End of - Headers==============================
I have a student interested in looking at carbon nanotubes and experimenting with them. Other than synthesizing them on-site, are there any sources for a small quantity that I could purchase at a reasonable price?
Thanks,
Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 27 -- From kraftpiano-at-gmail.com Fri May 16 13:48:54 2008 4, 27 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.241]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GImrwU017141 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 13:48:54 -0500 4, 27 -- Received: by rv-out-0708.google.com with SMTP id k29so424333rvb.30 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 11:48:53 -0700 (PDT) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=EzkgACEW70B8JNrgxDqAlLFkBQRXdKvgtNNcr910N+w=; 4, 27 -- b=UgtQcnt7J4NGHD3k4R2iJH7Y2WsvghS28qJk/WDyLO5nbjjNFomZrae/wQmyNxytwWyOmDAHkYIwZpEjVkiKmni3cUWthsrP3LI1EtWKViUdt03g12Al2UR4ofHDTpbjTxKi0/HFW0gBYAcwXhSeaH67jPVlw38ACNerXE5jTcQ= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=x3aGHrPZMsbhFYcgljn5VpN5ccc8boKZBtqDg0Z6XhCk3cuArVY3tk4sJFJYocYatc+6WxuG8yu30ivKq2rq1djqZHLWLypdNLbHRYipL3FC848MUbsf3rNvXPfEz2Vvr0T6xY3Nq5fH4NiZEhQlNCvoX3ftndm37+y8dVsTCdM= 4, 27 -- Received: by 10.141.22.1 with SMTP id z1mr1983625rvi.67.1210963733272; 4, 27 -- Fri, 16 May 2008 11:48:53 -0700 (PDT) 4, 27 -- Received: by 10.140.161.11 with HTTP; Fri, 16 May 2008 11:48:53 -0700 (PDT) 4, 27 -- Message-ID: {25e2b0d20805161148l6dd737c7g3112c721c2453b08-at-mail.gmail.com} 4, 27 -- Date: Fri, 16 May 2008 14:48:53 -0400 4, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: Carbon nanotube source. 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Have you considered contacting other universities? Center for Nanoscale Science at Rice University for instance?
You could also try contacting Mike Foley at Cheap Tubes. He publishes his CNT prices on his website, cheaptubes.com. I have no idea how he certifies the quality/purity of his products and this contact information is not to be construed as an endorsement by Smartimtech.
Regards,
Ira Bleiweiss Smart Imaging Technologies 713.589.3500 ira-at-simagis.us www.smartimtech.com
Advanced Software Solutions for Science and Industry
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Friday, May 16, 2008 1:56 PM To: ira-at-simagis.us
You can get many types of single and multi-walled CNTs from:
http://www.nanoamor.com/
I've bought several types. They are located in Los Alamos, NM and have an office in Houston, TX. My last buy was 1234NMG, 5g, $80. This type is 60-100nm and is used for SEM testing and IC applications. You would likely want a larger size.
gary g.
At 12:11 PM 5/16/2008, you wrote: } -----Original Message----- } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } Sent: Friday, May 16, 2008 1:56 PM } Subject: [Microscopy] Carbon nanotube source. } } I have a student interested in looking at carbon nanotubes and } experimenting with them. Other than synthesizing them on-site, are } there any sources for a small quantity that I could purchase at a } reasonable price? } } Thanks, } } Justin A. Kraft
==============================Original Headers============================== 8, 20 -- From gary-at-gaugler.com Fri May 16 15:36:35 2008 8, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4GKaZ4Z015052 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 15:36:35 -0500 8, 20 -- Message-Id: {200805162036.m4GKaZ4Z015052-at-ns.microscopy.com} 8, 20 -- Received: (qmail 32386 invoked from network); 16 May 2008 13:36:34 -0700 8, 20 -- Received: by simscan 1.1.0 ppid: 32381, pid: 32384, t: 0.1487s 8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 8, 20 -- by smtp1 with SMTP; 16 May 2008 13:36:34 -0700 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 20 -- Date: Fri, 16 May 2008 13:36:31 -0700 8, 20 -- To: kraftpiano-at-gmail.com 8, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 20 -- Subject: Re: [Microscopy] RE: Carbon nanotube source. 8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 20 -- In-Reply-To: {200805161911.m4GJBBl3000949-at-ns.microscopy.com} 8, 20 -- References: {200805161911.m4GJBBl3000949-at-ns.microscopy.com} 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-443D2336 ==============================End of - Headers==============================
We have several researchers who need to coat some wafers with 1-2 micrometers of aluminum. We tried using our conventional technique (10 cm of 10 mil Al wire evaporated from a tri-wire tungsten filament) but can only obtain extremely thin layers. What should we be doing to obtain the thick layers?
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
} From mail-at-ns.microscopy.com Fri May 16 14:50:51 2008 } Date: Fri, 16 May 2008 13:49:42 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: kraftpiano-at-gmail.com } Reply-to: kraftpiano-at-gmail.com } Subject: [Microscopy] Carbon nanotube source. } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have a student interested in looking at carbon nanotubes and } experimenting with them. Other than synthesizing them on-site, are } there any sources for a small quantity that I could purchase at a } reasonable price? } } Thanks, } } Justin A. Kraft } } -- } "America believes in education; the average professor earns more money } in a year than a professional athlete earns in a whole week." Evan } Esar
==============================Original Headers============================== 8, 12 -- From jquinn-at-www.matscieng.sunysb.edu Fri May 16 16:36:34 2008 8, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 8, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GLaW30009995 8, 12 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 16:36:33 -0500 8, 12 -- Received: (from jquinn-at-localhost) 8, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m4GLbY527369 8, 12 -- for microscopy-at-microscopy.com; Fri, 16 May 2008 17:37:34 -0400 8, 12 -- Date: Fri, 16 May 2008 17:37:34 -0400 8, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 8, 12 -- Message-Id: {200805162137.m4GLbY527369-at-www.matscieng.sunysb.edu} 8, 12 -- To: microscopy-at-microscopy.com 8, 12 -- Subject: re: nanotubes ==============================End of - Headers==============================
On May 16, 2008, at 2:18 PM, bozzola-at-siu.edu wrote:
} We have several researchers who need to coat some wafers with 1-2 } micrometers of aluminum. We tried using our conventional technique } (10 cm of 10 mil Al wire evaporated from a tri-wire tungsten } filament) but can only obtain extremely thin layers. What should we } be doing to obtain the thick layers?
Dear John, I had success producing thick Al layers by evaporating Al foil in a basket of W, which can be obtained from a number of EM supply houses. The basket has two arms and is conical with the wire wound to form the part that a ball of crumpled foil will fit into. I think that one could use as much foil as necessary, since Al has a relatively low boiling point. (The same is not true for Ti, which can melt and short enough W wire that the temperature never achieves the } 3000 K necessary to evaporate it.) I cannot say how flat or uniform the layer is except that Al evaporated onto plate glass makes a good mirror. You could also evaporate as usual, then repeat until a 1-2 um layer is built up, but this may not be practical unless you can get ~100 nm or more per individual layer. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri May 16 17:44:03 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4GMi3sS026060 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 May 2008 17:44:03 -0500 6, 22 -- Received: from earth-dog.its.caltech.edu (earth-dog [192.168.1.3]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id BA98E1BA74 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 May 2008 15:44:01 -0700 (PDT) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 793CE1B9B9 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 May 2008 15:43:59 -0700 (PDT) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200805162118.m4GLIell029414-at-ns.microscopy.com} 6, 22 -- References: {200805162118.m4GLIell029414-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {2447C9C0-27DD-4944-B8ED-7643182FF77D-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] evaporating thick aluminum layers 6, 22 -- Date: Fri, 16 May 2008 15:44:04 -0700 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.4.5 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Calculating Vascular Density and Length
Question: I am studying vascular network in a in vivo flatmounted diseased mouse model. With fluorescence, it easy to see that a difference in vascular density between the diseased mouse and the normal mouse. What is the best software and approach to measure vessel length and density? -Eunice
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Email: gcathro-at-nibsc.ac.uk Name: Gill Cathro
Organization: NIBSC
Title-Subject: [Filtered] NIBSC Cryo-EM Workshop
Question: JEOL, Leica/Bal-Tec and NIBSC are holding a dedicated Cryo-EM Workshop based on current generation cryo-EM technologies installed at the NIBSC Imaging Laboratory (South Mimms, Herts, UK). The course will be held over 5 days commencing Monday 22nd September to Friday 26th September 2008.
The Workshop will embrace cryo-preparation techniques using Cryo-FEGSEM, Cryo-TEM with tomography and the many associated preparation techniques: Freeze fracture (Bal-Tec), High pressure freezing, Cryo-sectioning, Freeze substitution, High resolution Cryo-FEG, Immuno-labelling
The ethos of the course is very much ëhands oní with technical support lectures from Scientists in these fields and application support from JEOL, Bal-Tec and EM Systems.
Registration Form and Workshop Programme: Can be downloaded from NIBSC website: www.nibsc.ac.uk/emconference.html
For any further information please contact Gill Cathro on: gcathro-at-nibsc.ac.uk
Thermal evaporation of aluminum from W baskets is problematic. The molten Al reacts with the W to form a eutectic (Al-W dendrite sample anyone!) and the W wire often breaks before you get a very thick film.
Also, when making thick (} 100nm) metal films, internal stress in the film becomes a problem. I've had films peel from the substrate due to the stress. I'm not sure if this is more of a problem with thermally evaporated films compared to sputtered films or not. Perhaps someone more familiar with the deposition processes can further clarify the issue. Since thick films are grown, there must be ways to do it.
Would it be better to grow a thick film by electro-deposition similar to the way they grow TEM grids?
Cheers, Henk
At 06:46 PM 5/16/2008, tivol-at-caltech.edu wrote:
} On May 16, 2008, at 2:18 PM, bozzola-at-siu.edu wrote: } } } We have several researchers who need to coat some wafers with 1-2 } } micrometers of aluminum. We tried using our conventional technique } } (10 cm of 10 mil Al wire evaporated from a tri-wire tungsten } } filament) but can only obtain extremely thin layers. What should we } } be doing to obtain the thick layers? } } } Dear John, } I had success producing thick Al layers by evaporating Al foil in a } basket of W, which can be obtained from a number of EM supply } houses. The basket has two arms and is conical with the wire wound } to form the part that a ball of crumpled foil will fit into. I think } that one could use as much foil as necessary, since Al has a } relatively low boiling point. (The same is not true for Ti, which } can melt and short enough W wire that the temperature never achieves } the } 3000 K necessary to evaporate it.) I cannot say how flat or } uniform the layer is except that Al evaporated onto plate glass makes } a good mirror. You could also evaporate as usual, then repeat until } a 1-2 um layer is built up, but this may not be practical unless you } can get ~100 nm or more per individual layer. } Yours, } Bill Tivol, PhD } EM Scientist } Electron Cryo-Microscopy Facility } Broad Center, Mail Code 114-96 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 6, 22 -- From tivol-at-caltech.edu Fri May 16 17:44:03 2008 } 6, 22 -- Received: from outgoing-mail.its.caltech.edu } (outgoing-mail.its.caltech.edu [131.215.239.19]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m4GMi3sS026060 } 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 May } 2008 17:44:03 -0500 } 6, 22 -- Received: from earth-dog.its.caltech.edu (earth-dog [192.168.1.3]) } 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id BA98E1BA74 } 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 May } 2008 15:44:01 -0700 (PDT) } 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu } [131.215.2.133]) } 6, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 793CE1B9B9 } 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 16 May } 2008 15:43:59 -0700 (PDT) } 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) } 6, 22 -- In-Reply-To: {200805162118.m4GLIell029414-at-ns.microscopy.com} } 6, 22 -- References: {200805162118.m4GLIell029414-at-ns.microscopy.com} } 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 6, 22 -- Message-Id: {2447C9C0-27DD-4944-B8ED-7643182FF77D-at-caltech.edu} } 6, 22 -- Content-Transfer-Encoding: 7bit } 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 6, 22 -- Subject: Re: [Microscopy] evaporating thick aluminum layers } 6, 22 -- Date: Fri, 16 May 2008 15:44:04 -0700 } 6, 22 -- To: microscopy-at-msa.microscopy.com } 6, 22 -- X-Mailer: Apple Mail (2.753) } 6, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.4.5 } ==============================End of - Headers==============================
Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility (614) 292-0674 040 Fontana Labs, 116 W. 19th Ave www.ceof.ohio-state.edu
==============================Original Headers============================== 11, 24 -- From colijn.1-at-osu.edu Fri May 16 19:15:27 2008 11, 24 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4H0FRw5012728 11, 24 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 19:15:27 -0500 11, 24 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 11, 24 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31224) 11, 24 -- id {01MUVAI4U80G8Y102H-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 11, 24 -- Fri, 16 May 2008 20:15:26 -0400 (EDT) 11, 24 -- Received: from HOC3.osu.edu (d118-75-42-25.try.wideopenwest.com [75.118.25.42]) 11, 24 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31224) 11, 24 -- with ESMTPA id {01MUVAI47FO28Y3CO3-at-ecr6.ohio-state.edu} ; Fri, 11, 24 -- 16 May 2008 20:15:26 -0400 (EDT) 11, 24 -- Date: Fri, 16 May 2008 20:14:59 -0400 11, 24 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 24 -- Subject: Re: [Microscopy] Re: evaporating thick aluminum layers 11, 24 -- In-reply-to: {200805162246.m4GMkWHn028454-at-ns.microscopy.com} 11, 24 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 24 -- To: tivol-at-caltech.edu, Microscopy Listserver {microscopy-at-microscopy.com} 11, 24 -- Message-id: {7.0.1.0.2.20080516195436.010f0c18-at-osu.edu} 11, 24 -- MIME-version: 1.0 11, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 24 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 24 -- References: {200805162246.m4GMkWHn028454-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: wcrgs-at-aol.com Name: R Obie
Organization: WCRG
Title-Subject: [Filtered] Microscope identification and user manual
Question: Hello all. We have come across what appears to be a very nice microscope; appears to be manufactured by Leitz Wetzlar. Product has some identifying statements and numbers; Germany 618782 A02 1172 (taped on). A Polaroid MP-4 camera was attached. Product uses one interchangeable objective at a time and has different condensers. Images of the microscope can be found by opening internet explorer and going to ftp://www.woodcoatingsresearchgroup.com/ Type in Username - wcrg-customer1 password-wcrg1102
My questions are: Can anyone identify this scope on have experience with it? Does anyone have a manual for this scope and its accessories? Can anyone offer suggestions for attaching a digital camera? Can anyone offer suggestions for obtaining additional objectives? I am particularly interested in } 50X.
You have a Leitz Otholux set up for but transmited and reflected polarized light. They made from 1934 thoug 1974. I have the documentation for it at www.science-info.net/docs/leitz I think it has been fitted with more modern lamp housings. I may have the documentation for them as well.
Look all all the documentation labeled Otholux. The zip files are duplicates of large PDF files that can be used if the pdf files hang up on down load. Also look at anything that references polarized light as well.
I also have much more documentation for many brands of scopes and links to more on the site: www.science-info.net
It is free to all that need it.
While the stand looks like its had a long life and a lot of use if any scope will stand up to it this one will. Even though it has been out of production for 34 years I still see new parts for them and there are plenty of good used parts available world wide.
Refurbished ones are still being sold to do research today by researchers on a limited budgets or one that need attachments not available on modern scopes. It wold not surprise me to find Otholuxes in labs still doing their jobs 100 years from now if I were still alive. They are that well built and there are that many parts still out there. I doubt that is true of any other scope.
I have seen many things built using an Otholux as starting point. In fact the International Space Station uses the fine focus block from one for something they could find a mondern device to do.
best wishes Gordon Couger Stillwater, OK
----- Original Message ---- X-from: "wcrgs-at-aol.com" |--wcrgs-at-aol.com--| To: gcouger-at-science-info.net Sent: Saturday, May 17, 2008 3:14:31 PM
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Email: wcrgs-at-aol.com Name: R Obie
Organization: WCRG
Title-Subject: [Filtered] Microscope identification and user manual
Question: Hello all. We have come across what appears to be a very nice microscope; appears to be manufactured by Leitz Wetzlar. Product has some identifying statements and numbers; Germany 618782 A02 1172 (taped on). A Polaroid MP-4 camera was attached. Product uses one interchangeable objective at a time and has different condensers. Images of the microscope can be found by opening internet explorer and going to ftp://www.woodcoatingsresearchgroup.com/ Type in Username - wcrg-customer1 password-wcrg1102
My questions are: Can anyone identify this scope on have experience with it? Does anyone have a manual for this scope and its accessories? Can anyone offer suggestions for attaching a digital camera? Can anyone offer suggestions for obtaining additional objectives? I am particularly interested in --|50X.
I.P.S. GILL has invited you to join the 'Electron Microscopy & Nano Lab' community.
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Best wishes,
Gill
-- Room No. 37, Electron Microscopy & Nanoscience Laboratory, PUNJAB AGRICULTURAL UNIVERSITY LUDHIANA 141 004 INDIA Tel: (+91) 161 2401960-79 Ext. 288 Web: www.pau.edu/emnl Mobile: +91-987-877-2577
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I.P.S. GILL has invited you to join the 'Electron Microscopy & Nano Lab' community.
To view the details of 'Electron Microscopy & Nano Lab' community page, visit: http://www.orkut.com/Community.aspx?cmm=51394277
-------------------------------------------------------------------------------------------------------------------------- Kindly click on the link above and join the community.
Best wishes,
Gill
-- Room No. 37, Electron Microscopy & Nanoscience Laboratory, PUNJAB AGRICULTURAL UNIVERSITY LUDHIANA 141 004 INDIA Tel: (+91) 161 2401960-79 Ext. 288 Web: www.pau.edu/emnl Mobile: +91-987-877-2577
-- Room No. 37, Electron Microscopy & Nanoscience Laboratory, PUNJAB AGRICULTURAL UNIVERSITY LUDHIANA 141 004 INDIA Tel: (+91) 161 2401960-79 Ext. 288 Web: www.pau.edu/emnl Mobile: +91-987-877-2577
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Please note that the recent posting by IPGill is a link to a social networking environment. It has no obvious microscopy connection. I would strongly suggest that you ignore this.
I have removed IPGILL from authorized subscribers listing.
Nestor Your Friendly Neighborhood SysOp.
==============================Original Headers============================== 4, 11 -- From zaluzec-at-microscopy.com Sun May 18 09:21:01 2008 4, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 4, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4IEKxDn029713 4, 11 -- for {microscopy-at-microscopy.com} ; Sun, 18 May 2008 09:21:00 -0500 4, 11 -- Mime-Version: 1.0 4, 11 -- Message-Id: {p06240801c455ea489d1a-at-[206.69.208.22]} 4, 11 -- Date: Sun, 18 May 2008 09:20:59 -0500 4, 11 -- To: microscopy-at-microscopy.com 4, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 4, 11 -- Subject: Administrivia: Warning: Recent Invitation 4, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I need a camera lucida for making drawings of silver stained preparations. I was wandering if any of you could know where I can get one. I looked for in the web but I only found a provider of prism camera lucida for student microscopes. I have and Axiscop Zeiss microscope and don´t know if this kind of camera lucida can be also used with it. Another possibility could be that in any of your institutions there is one that is no longer in use...?
Best wishes
María E. Castelló, PhD Associate Scientist Integrative and Computational Neuroscience Department Instituto de Investigaciones Biológicas Clemente Estable Montevideo URUGUAY
==============================Original Headers============================== 5, 28 -- From maritacastello-at-gmail.com Sun May 18 20:48:59 2008 5, 28 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.190]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4J1mxCC018694 5, 28 -- for {microscopy-at-microscopy.com} ; Sun, 18 May 2008 20:48:59 -0500 5, 28 -- Received: by fk-out-0910.google.com with SMTP id 18so1675516fks.2 5, 28 -- for {microscopy-at-microscopy.com} ; Sun, 18 May 2008 18:48:58 -0700 (PDT) 5, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 28 -- d=gmail.com; s=gamma; 5, 28 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 28 -- bh=5H4NyIqoU41nk30xAiEWmCMKeuaaeTO8Sowgh7jLDxo=; 5, 28 -- b=No1cLKkHzPhozgtLy9CoQ8Sp5gobppHyIy6yJi7hZcOJCetXjeAzwoJs+CxPDFxoc45p+dPCeXQghtY+Vg9fz1D0noUExB9WTZmwvmWmuEOImWmOcsakR4mP9Y0cRPBcvawOHiwtYDi1btx522ZkNyznFWCf0BLYvvxjGtCEoHQ= 5, 28 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 28 -- d=gmail.com; s=gamma; 5, 28 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 28 -- b=o+sFFSBqt8P8hAYx46mqL+158PaPrlf6PhXXmOxKGFFwWJgZb/y0z8fs/93oT3m928E+Ow0NsYPMwI88Yefv2JsKg3xNRgeytebNfI93zlcqxO7OR1OqGnsZBfuHlCcb+e7UmG7MS2KspYcxi7nEuM9o5+/QbU2Y8IuVZyB0AaY= 5, 28 -- Received: by 10.125.82.5 with SMTP id j5mr5413982mkl.48.1211161738455; 5, 28 -- Sun, 18 May 2008 18:48:58 -0700 (PDT) 5, 28 -- Received: by 10.86.9.19 with HTTP; Sun, 18 May 2008 18:48:58 -0700 (PDT) 5, 28 -- Message-ID: {42767f520805181848q76367aeexf02cb59b9991df43-at-mail.gmail.com} 5, 28 -- Date: Sun, 18 May 2008 22:48:58 -0300 5, 28 -- From: "Maria Castello" {maritacastello-at-gmail.com} 5, 28 -- To: microscopy-at-microscopy.com 5, 28 -- Subject: camera lucida for Axioscop Zeiss microscope 5, 28 -- MIME-Version: 1.0 5, 28 -- Content-Type: text/plain; charset=ISO-8859-1 5, 28 -- Content-Disposition: inline 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4J1mxCC018694 ==============================End of - Headers==============================
I forgot to mention a particular ‘hazard’ of long term storage of frozen biopsy tissue, even under even liquid nitrogen or at -70oC, which is the sample drying out (sublimation of the ice crystals). Sometimes we want this and call it freeze drying other times we don’t and call it desiccation. Often you would normally want to prevent the desiccation of soft tissue samples during their storage while frozen, although I have freeze dried cut sections for preservation and use it for tissue samples like bone (I’ve even seen human lung tissue preserved quite well by drying a small cube of lung in the sun – although I don’t have the protocol).
Embedding the tissue in cryo-wax (cryostat OCT compound) helps reduce freeze-drying at -20oC or even lower. We did get problems with sectioning soft tissue when it was stored for over a month in air inside a cryotube under liquid nitrogen, but this was snap-frozen unfixed and no cryo-protectant (and we didn’t intend storing them for that long). Presumably your fixed tissue would be stored in a similar manner (i.e. fairly dry, so that it snap freezes very rapidly) and so will be subject to freeze drying - the effects of which can range from beneficial (preservation) to harmful (physical distortion and chemical disruption). Some wrap the sample in plastic film or tin foil to minimise freeze drying (and use cryo-wax OCT*), although this does make handling more difficult afterwards. Certainly you need to seal the tissue in something with no air spaces for long-term storage. The cryostat OCT embedding wax itself will also ‘freeze dry’ if stored poorly, making the tissue block pretty impossible to section afterwards*. Again this is also a complex issue that should be thought through before the long term storage of frozen samples.
Regards
Keith
*e.g. http://www.bristol.ac.uk/vetpath/cpl/cryostat.html#Pictures --------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 14 May 2008 11:10 To: kjmorris-at-well.ox.ac.uk
Dear all, I have been asked to found the best method to freeze entire rat organs for an undetermined period of time, maybe years. They may be recovered any time for histology observation. I told the people that it was probably better to leave them in a fixative at 4°C but they want them frozen. I am not very experienced in cryopreservation (except for cells in culture), especially of whole organs. For me the ideal way would be to perfuse with glycerol (I seem to remember something like 60%) but I simply wonder if we master the technique to cryopreserve whole rat organs. Fact is, here we need only to maintain the structure, not the activity! Problem is, the organs cannot be perfused. They will be fixed in formalin and given to me whole. If anyone has experience or infos to share, they are welcome. Stephane
P.S: don't laugh: they just wanted to quick-freeze them by directly plunging the organs in liquid nitrogen!
==============================Original Headers============================== 5, 21 -- From nizets2-at-yahoo.com Wed May 14 04:58:21 2008 5, 21 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4E9wJBu013979 5, 21 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2008 04:58:20 -0500 5, 21 -- Received: (qmail 52964 invoked by uid 60001); 14 May 2008 09:58:18 -0000 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 21 -- s=s1024; d=yahoo.com; 5, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Ty pe:Content-Transfer-Encoding:Message-ID; 5, 21 -- b=Ugb9vRZAp8URzwCI3wPnghgAYSea9wVL7Ns9i23nrZtzYMfvs8axWVV0CnBNS+Q7ujDZPm8ab9 CLkBivhz8DOfRup4644oJHeVLiiWgPGecRP9Q1MdqHtTJn0t8Gej7IE31WJtYnsAiN+nqmx3uAF0 mNTY6pPpiIWlw0qT1O5AM=; 5, 21 -- X-YMail-OSG: r.1ABgkVM1nSNshliAQjRtqrpjanTBdVflfVjg59iUVM4_mafkpP66FQpKMn7PN1HppsueOzhNCa MjanTQLz.axgQShfpB2rJF2jZw-- 5, 21 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Wed, 14 May 2008 02:58:18 PDT 5, 21 -- X-Mailer: YahooMailRC/975.23 YahooMailWebService/0.7.185 5, 21 -- Date: Wed, 14 May 2008 02:58:18 -0700 (PDT) 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 21 -- Subject: =?iso-8859-1?Q?Storage_of_organs_at_-=B0C?= 5, 21 -- To: microscopy-at-microscopy.com 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 5, 21 -- Message-ID: {636315.52694.qm-at-web37408.mail.mud.yahoo.com} 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4E9wJBu013979 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 21 -- From kjmorris-at-well.ox.ac.uk Mon May 19 08:11:14 2008 30, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 30, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JDBDKf032247 30, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 May 2008 08:11:14 -0500 30, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 30, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 30, 21 -- id 1Jy58z-0003Ez-1W 30, 21 -- for Microscopy-at-Microscopy.Com; Mon, 19 May 2008 14:11:13 +0100 30, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 30, 21 -- To: {Microscopy-at-Microscopy.Com} 30, 21 -- Subject: =?iso-8859-1?Q?FW:_=5BMicroscopy=5D_Storage_of_organs_at_-=B0C?= 30, 21 -- Date: Mon, 19 May 2008 14:13:13 +0100 30, 21 -- Message-ID: {000f01c8b9b2$17c0fce0$b22c4381-at-CytoWhizz} 30, 21 -- MIME-Version: 1.0 30, 21 -- Content-Type: text/plain; 30, 21 -- charset="iso-8859-1" 30, 21 -- X-Mailer: Microsoft Office Outlook 11 30, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 30, 21 -- Thread-Index: Aci5shefwPe18dhcQeemlYK3XQsFlA== 30, 21 -- Content-Transfer-Encoding: 8bit 30, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4JDBDKf032247 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (rpsmith-at-esf.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, May 19, 2008 at 09:50:29 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both rpsmith-at-esf.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: rpsmith-at-esf.edu Name: Robert P. Smith, MS
Organization: SUNY ESF
Education: Graduate College
Location: Syracuse, NY USA
Title: LR Gold
Question: Lately, I had trouble UV polymerizing samples in LR Gold. Sometimes, the samples are soft and rubbery in the plastic, other times the plastic is cloudy and when cut, numerous small holes are present. What am I doing wrong??
Until you get whatever purification system you decide upon, may I suggest that Molecular Grade purified water seems an overkill? I understand it is certified RNAse free, or something to that effect, and so costs at least 2x as much as other lab bottled purified water sold. The one we use is labeled "distilled deionized"; it has been working well for EM.
Hope this saves you some small $$ in the meanwhile. Let me know if you are interested what exactly the label on our water says. Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Begin forwarded message:
} From: duleyml-at-muohio.edu } Date: May 15, 2008 7:14:26 PM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] viaWWW: "Pure" water?? } Reply-To: duleyml-at-muohio.edu } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both duleyml-at-muohio.edu as well as the MIcroscopy } Listserver } ---------------------------------------------------------------------- } ----- } } Email: duleyml-at-muohio.edu } Name: Matt Duley } } Organization: Miami University } } Title-Subject: [Filtered] "Pure" water?? } } Question: Good afternoon all, } } We are a multiuser EM and Light microscopy } facility. We are going to be moving our facility } across campus in the next year (or so) and will } be losing easy access to the Nanopure water } system we have been using. We use the Nanopure } water for rinses etc. The water here in Oxford } is not good, we get contaminants even with the } Nanopure system that show up at the TEM level. } We have begun using ìmolecular gradeî water from } Fischer Scientific for our fixatives and stains, } UA, lead citrate etc. When we made the switch to } the molecular grade water for our fixatives and } stains most of the problems went away. } Obviously, that stuff is too expensive to use for } rinses and general use. My question is this, } would it be worth our while to pursue a glass } still, (there is a ìbrokenî one in another dept., } heating element I think) to take with us when we } move, or do we get another Nanopure system? What } are your labs doing for ìpureî water? } } Your thoughts? } } Matthew L. Duley } } Electron Microscopist } Electron Microscopy Facility } 359 Pearson Hall } Miami University } Oxford, Ohio 45056 } 513.529.4164 } FAX 513.529.4243 } Duleyml-at-muohio.edu } } Login Host: 134.53.14.105 } ---------------------------------------------------------------------- } ----- } } } ==============================Original } Headers============================== } 11, 13 -- From zaluzec-at-microscopy.com Thu May 15 18:13:21 2008 } 11, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 11, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m4FNDJvx032281 } 11, 13 -- for {microscopy-at-microscopy.com} ; Thu, 15 May 2008 } 18:13:21 -0500 } 11, 13 -- Mime-Version: 1.0 } 11, 13 -- Message-Id: {p06240801c4527400e005-at-[206.69.208.22]} } 11, 13 -- Date: Thu, 15 May 2008 18:13:17 -0500 } 11, 13 -- To: microscopy-at-microscopy.com } 11, 13 -- From: duleyml-at-muohio.edu (by way of MicroscopyListserver) } 11, 13 -- Subject: viaWWW: "Pure" water?? } 11, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; } format="flowed" } 11, 13 -- Content-Transfer-Encoding: 8bit } 11, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4FNDJvx032281 } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 24 -- From vladislav_speransky-at-nih.gov Mon May 19 13:31:18 2008 9, 24 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JIVHII009317 9, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 13:31:17 -0500 9, 24 -- X-IronPortListener: NIH_Relay 9, 24 -- X-SBRS: None 9, 24 -- X-IronPort-AV: E=Sophos;i="4.27,511,1204520400"; 9, 24 -- d="scan'208";a="192656810" 9, 24 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 9, 24 -- by nihrelayxway4.hub.nih.gov with ESMTP; 19 May 2008 14:24:37 -0400 9, 24 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 9, 24 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m4JIOb7R14659970 9, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 14:24:37 -0400 (EDT) 9, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 9, 24 -- References: {200805152314.m4FNEQ6o001314-at-ns.microscopy.com} 9, 24 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 24 -- Message-Id: {99F32416-259C-408A-A9A7-7B1FA3C1ED5F-at-nih.gov} 9, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 9, 24 -- Subject: Fwd: [Microscopy] viaWWW: "Pure" water?? 9, 24 -- Date: Mon, 19 May 2008 14:24:34 -0400 9, 24 -- To: Microscopy-at-microscopy.com 9, 24 -- X-Mailer: Apple Mail (2.753) 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4JIVHII009317 ==============================End of - Headers==============================
I hope someone can point me in the right direction.
We have a post-doc using our facility that has been searching for a specific type of material to wick solutions from her specimen grids. She says that in the past, she had used something that drew fluids away slowly, such that her sample (specifically suspended particulates) would safely "lower" onto the mesh (or film substrate) without being sucked off the grid and absorbed by the wicking material. (Because of the protocol she used, she couldn't wait each time for the solution to evaporate.) She didn't think it was filter paper, and described it as coming in rectangular strips and being just brittle enough that it would "snap" if you bent it, leaving clean edges without loose fibers to potentially contaminate the grids. She tried contacting the technician that originally showed her this, but that person didn't know (or couldn't remember) what the product was.
I would also be interested in this product for similar reasons (sometimes filter paper and dental paper points are much too fast and evaporation is too slow). Does anyone have any suggestions?
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
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==============================Original Headers============================== 10, 23 -- From LettJ-at-ent.wustl.edu Mon May 19 15:37:23 2008 10, 23 -- Received: from MAIL3.wusm-pcf.wustl.edu (mail3.wusm-pcf.wustl.edu [128.252.17.170]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JKbN1u028867 10, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 15:37:23 -0500 10, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL3.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Mon, 19 May 2008 15:36:35 -0500 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="us-ascii" 10, 23 -- Subject: TEM: wicking or blotting grids 10, 23 -- Date: Mon, 19 May 2008 15:37:22 -0500 10, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C390B1B7F-at-EX04.wusm-pcf.wustl.edu} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: TEM: wicking or blotting grids 10, 23 -- Thread-Index: Aci58B/0iRZZmd/WS+GoUZ5OhYbycQ== 10, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 10, 23 -- To: {Microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 19 May 2008 20:36:35.0037 (UTC) FILETIME=[079230D0:01C8B9F0] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4JKbN1u028867 ==============================End of - Headers==============================
Whenever I had something like that being necessary, for instance for removing surplus methylcellulose over ultrathin cryosections I used wet filter paper, or wet tissue. That will remove the solution to be blotted off very slowly.
Good luck!
Jan Leunissen
Aurion - President Costerweg 5 6702 AA Wageningen The Netherlands phone 31-317-497676 fax 31-317-415955 http://www.aurion.nl
==============================Original Headers============================== 5, 24 -- From leunissen-at-aurion.nl Mon May 19 15:53:27 2008 5, 24 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JKrPSH009832 5, 24 -- for {microscopy-at-microscopy.com} ; Mon, 19 May 2008 15:53:26 -0500 5, 24 -- Received: from mta05.xtra.co.nz ([172.23.11.51]) by fep01.xtra.co.nz 5, 24 -- with ESMTP 5, 24 -- id {20080519205325.ZSET14436.fep01.xtra.co.nz-at-mta05.xtra.co.nz} ; 5, 24 -- Tue, 20 May 2008 08:53:25 +1200 5, 24 -- Received: from [192.168.1.50] (really [122.57.247.121]) by mta05.xtra.co.nz 5, 24 -- with ESMTP 5, 24 -- id {20080519205325.VKLJ8207.mta05.xtra.co.nz-at-[192.168.1.50]} ; 5, 24 -- Tue, 20 May 2008 08:53:25 +1200 5, 24 -- In-Reply-To: {200805192037.m4JKbmbJ029239-at-ns.microscopy.com} 5, 24 -- References: {200805192037.m4JKbmbJ029239-at-ns.microscopy.com} 5, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 24 -- Message-Id: {659B651B-A4E2-464A-B76C-AB31E532883F-at-aurion.nl} 5, 24 -- Cc: microscopy-at-microscopy.com 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- From: Jan Leunissen {leunissen-at-aurion.nl} 5, 24 -- Subject: [Microscopy] Re: TEM: wicking or blotting grids 5, 24 -- Date: Tue, 20 May 2008 08:53:23 +1200 5, 24 -- To: LettJ-at-ent.wustl.edu 5, 24 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
I am looking for a stage that makes it possible to look at an object from all sides, in other words, one that can be rotated along the three possible axes independently. I have found one option, produced by keyence, but I would like to find other solutions, preferably ones that are more within the budget range that we have. Can anyone help?
Kim -- http://www.kimvdlinde.com
==============================Original Headers============================== 3, 23 -- From kim-at-kimvdlinde.com Mon May 19 16:03:51 2008 3, 23 -- Received: from bio.fsu.edu (bio.fsu.edu [128.186.38.55]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JL3oD6023013 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 16:03:50 -0500 3, 23 -- Received: from [128.186.178.252] (illegally-used-at.fsu [128.186.178.252] (may be forged)) 3, 23 -- by bio.fsu.edu (8.13.8/8.13.8) with ESMTP id m4JKnPgS013385 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 16:49:25 -0400 (EDT) 3, 23 -- (envelope-from kim-at-kimvdlinde.com) 3, 23 -- Message-ID: {4831EAE6.9050601-at-kimvdlinde.com} 3, 23 -- Date: Mon, 19 May 2008 17:02:30 -0400 3, 23 -- From: Kim van der Linde {kim-at-kimvdlinde.com} 3, 23 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 3, 23 -- MIME-Version: 1.0 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- Subject: Flexible stages with 6 degrees of freedom 3, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 23 -- Content-Transfer-Encoding: 7bit 3, 23 -- X-FSU-Biology-MailScanner-Information: Please contact astuy-at-bio.fsu.edu for more information 3, 23 -- X-FSU-Biology-MailScanner: Found to be clean 3, 23 -- X-FSU-Biology-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 3, 23 -- score=-1.8, required 4, autolearn=not spam, ALL_TRUSTED -1.80) 3, 23 -- X-FSU-Biology-MailScanner-From: kim-at-kimvdlinde.com 3, 23 -- X-Spam-Status: No ==============================End of - Headers==============================
If speed is an issue, you could try using 'paper points'. These are thin enough (there are different sizes so that you can pick the correct wicking speed) that you can have lots of control over the process.
Such as 71011-01 found at http://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx
David
On May 19, 2008, at 1:40 PM, LettJ-at-ent.wustl.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello, } } I hope someone can point me in the right direction. } } We have a post-doc using our facility that has been searching for a } specific type of material to wick solutions from her specimen grids. } She says that in the past, she had used something that drew fluids } away } slowly, such that her sample (specifically suspended particulates) } would } safely "lower" onto the mesh (or film substrate) without being sucked } off the grid and absorbed by the wicking material. (Because of the } protocol she used, she couldn't wait each time for the solution to } evaporate.) She didn't think it was filter paper, and described it as } coming in rectangular strips and being just brittle enough that it } would } "snap" if you bent it, leaving clean edges without loose fibers to } potentially contaminate the grids. She tried contacting the } technician } that originally showed her this, but that person didn't know (or } couldn't remember) what the product was. } } I would also be interested in this product for similar reasons } (sometimes filter paper and dental paper points are much too fast and } evaporation is too slow). Does anyone have any suggestions? } } Thank you, } } Jaci } } Jaclynn Lett } Senior Research Technician, EM Facility } Research Center for Auditory and Vestibular Studies } Department of Otolaryngology } Washington University School of Medicine } 660 S. Euclid Ave., Campus Box 8115 } St. Louis, MO 63110 } } Voice: 314-747-7257 } Fax: 314-747-7230 } Email: lettj-at-ent.wustl.edu } http://otocore.wustl.edu } } {br/} The materials in this message are private and may contain } Protected Healthcare Information. If you are not the intended } recipient, be advised that any unauthorized use, disclosure, } copying or the taking of any action in reliance on the contents of } this information is strictly prohibited. If you have received this } email in error, please immediately notify the sender via telephone } or return mail. } } } ==============================Original } Headers============================== } 10, 23 -- From LettJ-at-ent.wustl.edu Mon May 19 15:37:23 2008 } 10, 23 -- Received: from MAIL3.wusm-pcf.wustl.edu (mail3.wusm- } pcf.wustl.edu [128.252.17.170]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m4JKbN1u028867 } 10, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 } 15:37:23 -0500 } 10, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) } by MAIL3.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959); } 10, 23 -- Mon, 19 May 2008 15:36:35 -0500 } 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 10, 23 -- Content-class: urn:content-classes:message } 10, 23 -- MIME-Version: 1.0 } 10, 23 -- Content-Type: text/plain; } 10, 23 -- charset="us-ascii" } 10, 23 -- Subject: TEM: wicking or blotting grids } 10, 23 -- Date: Mon, 19 May 2008 15:37:22 -0500 } 10, 23 -- Message-ID: } {1C8B3F4710BBDD4CA24CB5269F2B4C390B1B7F-at-EX04.wusm-pcf.wustl.edu} } 10, 23 -- X-MS-Has-Attach: } 10, 23 -- X-MS-TNEF-Correlator: } 10, 23 -- Thread-Topic: TEM: wicking or blotting grids } 10, 23 -- Thread-Index: Aci58B/0iRZZmd/WS+GoUZ5OhYbycQ== } 10, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} } 10, 23 -- To: {Microscopy-at-microscopy.com} } 10, 23 -- X-OriginalArrivalTime: 19 May 2008 20:36:35.0037 (UTC) } FILETIME=[079230D0:01C8B9F0] } 10, 23 -- Content-Transfer-Encoding: 8bit } 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4JKbN1u028867 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 8, 22 -- From Elliott-at-arizona.edu Mon May 19 17:34:23 2008 8, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JMYMrj008024 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 19 May 2008 17:34:22 -0500 8, 22 -- Received: from gandalfs_amavis (amavis9.email.arizona.edu [10.0.0.237]) 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id A7C0064F0F9 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 19 May 2008 15:34:21 -0700 (MST) 8, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 8, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 3755564F0BB 8, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 19 May 2008 15:34:20 -0700 (MST) 8, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 8, 22 -- In-Reply-To: {200805192040.m4JKeiv6000949-at-ns.microscopy.com} 8, 22 -- References: {200805192040.m4JKeiv6000949-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 22 -- Message-Id: {8F9ABACF-5240-434C-9BDE-BA8F33570D40-at-arizona.edu} 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- From: David Elliott {Elliott-at-arizona.edu} 8, 22 -- Subject: Re: [Microscopy] TEM: wicking or blotting grids 8, 22 -- Date: Mon, 19 May 2008 15:34:15 -0700 8, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 8, 22 -- X-Mailer: Apple Mail (2.753) 8, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Disclaimer: SBT manufactures and sells wire saws and low speed diamond cut-off saws. I put this here because of the commercial nature of the information provided in answering the question, but I am also trying to put some general knowledge information in the answer to
South Bay Technology makes another diamond saw which holds the sample horizontally where the wire comes down to the sample. This is the model 850. Both the 810 and the 850 can use two types of wire blades, stainless steel and diamond impregnated. The stainless steel is used with an abrasive slurry, usually with boron carbide. Boron carbide is very hard and has a low density and stays in suspension very nicely. This will give the smoothest cut with the least damage. We have several application notes that discuss the kerf, cutting rates, cut quality compared to our low speed diamond saws using different blades. Basically, an abrasive slurry wire blade using a stainless steel wire will give you the smoothest cuts with the least amount of damage compared to a diamond impregnated, diamond coated, or a low speed diamond cut-off saw. The smallest stainless steel wire will also give the minimum kerf, so if you have a small sample size and you want to minimize loss, this is an option that does that.
On the sample holding mechanism: Our 810 is similar to our 650 and 660 low speed saws in having the sample upside down and delivered to the blade. There are a number of other competitors on the market that have a similar mechanism and these have worked quite well for many years. In addition, we and our competitors have developed a number of different types of holders for different types of samples that could alleviate your fears for your samples. I wouldn't worry about that aspect at all.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: s.miao-at-sheffield.ac.uk [mailto:s.miao-at-sheffield.ac.uk] Sent: Friday, May 16, 2008 6:10 AM To: Walck-at-SouthBayTech.com
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Email: s.miao-at-sheffield.ac.uk Name: Shu Miao
Title-Subject: [Filtered] Diamond wire saw
Question: Hello all, I'm looking for advice to buy a diamond wire saw. We will mostly to use it cut cross-section samples. Currently, we are looking at the WELL 3032 and South Bay Technology 810. I had pleasant experience with 3032 before. But the 810 also looks good. What I worry is the 810 holds the sample facedown. Will a small pallet comes off from the holder when cutting a cross-section stack? I encountered this problem when using a verticle saw like WELL 3242. Does anyone have recommendation on other brands? Thanks
Shu Miao Department of Engineering Materials University of Sheffield
This Question was submitted to Ask-A-Microscopist by (rpsmith-at-esf.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, May 19, 2008 at 14:42:37 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both rpsmith-at-esf.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: rpsmith-at-esf.edu Name: Robert P. Smith, MS
Organization: SUNY ESF
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Location: Syracuse, NY USA
Title: certification
Question: Does anyone know how an Electron Microscope Lab becomes certified?
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Email: ess26-at-runbox.com Name: Eddy S
Title-Subject: [Filtered] AFM Systems Cost
Question: Hi,
I am interested in buying an AFM system for our University Laboratory, and would like please to have an estimate about the likely costs in purchasing and setting up such a system. Also, it would nice to have a quick feedback on particular systems by users if possible.
The only materials I have experience with of that wet slowly and are brittle are nitrocellulose (and similar) membranes, used for protein/RNA/DNA blots or in micropore filters. The wet filter paper idea seems the simplest and cheapest, though!
cheers, Roseamry
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
==============================Original Headers============================== 6, 22 -- From prvs=Rosemary.White=0187dd7cf-at-csiro.au Mon May 19 18:37:02 2008 6, 22 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JNafei030054 6, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 18:36:56 -0500 6, 22 -- X-IronPort-AV: E=Sophos;i="4.27,511,1204462800"; 6, 22 -- d="scan'208";a="163828264" 6, 22 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 6, 22 -- by vic-ironport-int.csiro.au with ESMTP; 20 May 2008 08:21:10 +1000 6, 22 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 6, 22 -- Tue, 20 May 2008 08:21:08 +1000 6, 22 -- User-Agent: Microsoft-Entourage/11.0.0.040405 6, 22 -- Date: Tue, 20 May 2008 08:26:20 +1000 6, 22 -- Subject: Re: [Microscopy] Re: TEM: wicking or blotting grids 6, 22 -- From: Rosemary White {rosemary.white-at-csiro.au} 6, 22 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 6, 22 -- Message-ID: {C4583BAC.B6DB%rosemary.white-at-csiro.au} 6, 22 -- In-Reply-To: {200805192059.m4JKxkcw019612-at-ns.microscopy.com} 6, 22 -- Mime-version: 1.0 6, 22 -- Content-type: text/plain; 6, 22 -- charset="US-ASCII" 6, 22 -- Content-transfer-encoding: 7bit 6, 22 -- X-OriginalArrivalTime: 19 May 2008 22:21:09.0428 (UTC) FILETIME=[A3664740:01C8B9FE] ==============================End of - Headers==============================
Living multiple lives as both microscopist and molecular virologist I think i've probably used most options for doing negative staining, and have done all sorts of transblotting. The first thought would have been points or filter paper cut so that you had no loose fibres for wicking as with Dave Elliot's point. However, Rosemary's observation is probably best. Your description of the brittleness and flow rate for what the post-doc used fits something like Zetaprobe right down the line. Go over and borrow a little from your local contact in the molecular biology group and try it. It should do the job. If you don't have a contact, call Skip Virgin's group, I'm sure he would have some. Explain the problem and they should help out - he's actually an OK guy. Not sure how close you will be to him in the Wash U med school complex, but he is close.
Just discovered that there had been a change in the address from the list server. As a result my institution had decided that it's mail was spam. Couldn't figure out why I was only getting a few things, 1-2 weeks apart. Fixed it - as the line goes "I'm Back".
Paul
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca {mailto:paul_hazelton-at-umanitoba.ca} Phone:204-789-3313 (w); 204-489-6924 (h) Cell:204-781-6982 Fax:204-789-392
==============================Original Headers============================== 5, 22 -- From paul_hazelton-at-umanitoba.ca Mon May 19 19:32:37 2008 5, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4K0WaWm015219 5, 22 -- for {microscopy-at-microscopy.com} ; Mon, 19 May 2008 19:32:36 -0500 5, 22 -- Received: from [192.168.100.102] (wnpgmb014vw-ad05-245-173.dynamic.mts.net [205.200.245.173]) 5, 22 -- (authenticated bits=0) 5, 22 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m4K0WWNM029070 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 22 -- Mon, 19 May 2008 19:32:35 -0500 (CDT) 5, 22 -- Message-ID: {48321C23.1080401-at-umanitoba.ca} 5, 22 -- Date: Mon, 19 May 2008 19:32:35 -0500 5, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 22 -- Organization: University of Manitoba 5, 22 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 5, 22 -- MIME-Version: 1.0 5, 22 -- To: LettJ-at-ent.wustl.edu, Microscopy Listserver {microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] TEM: wicking or blotting grids 5, 22 -- References: {200805192039.m4JKdeAM031862-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200805192039.m4JKdeAM031862-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
Has anyone tried coating cryo-blocks with oil, as in the "Protective Oil" sold by Instrumedics? Have not tried this, but wondered if other sorts of oil would work as well? It's supposed to prevent dehydration.
cheers, Rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
On 19/5/08 11:20 PM, "kjmorris-at-well.ox.ac.uk" {kjmorris-at-well.ox.ac.uk} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi Stephane, } } I forgot to mention a particular Œhazard‚ of long term storage of frozen } biopsy tissue, even under even liquid nitrogen or at -70oC, which is the } sample drying out (sublimation of the ice crystals). Sometimes we want this } and call it freeze drying other times we don‚t and call it desiccation. } Often you would normally want to prevent the desiccation of soft tissue } samples during their storage while frozen, although I have freeze dried cut } sections for preservation and use it for tissue samples like bone (I‚ve even } seen human lung tissue preserved quite well by drying a small cube of lung } in the sun ˆ although I don‚t have the protocol). } } Embedding the tissue in cryo-wax (cryostat OCT compound) helps reduce } freeze-drying at -20oC or even lower. We did get problems with sectioning } soft tissue when it was stored for over a month in air inside a cryotube } under liquid nitrogen, but this was snap-frozen unfixed and no } cryo-protectant (and we didn‚t intend storing them for that long). } Presumably your fixed tissue would be stored in a similar manner (i.e. } fairly dry, so that it snap freezes very rapidly) and so will be subject to } freeze drying - the effects of which can range from beneficial } (preservation) to harmful (physical distortion and chemical disruption). } Some wrap the sample in plastic film or tin foil to minimise freeze drying } (and use cryo-wax OCT*), although this does make handling more difficult } afterwards. Certainly you need to seal the tissue in something with no air } spaces for long-term storage. The cryostat OCT embedding wax itself will } also Œfreeze dry‚ if stored poorly, making the tissue block pretty } impossible to section afterwards*. Again this is also a complex issue that } should be thought through before the long term storage of frozen samples. } } Regards } } Keith } } } *e.g. http://www.bristol.ac.uk/vetpath/cpl/cryostat.html#Pictures } --------------------------------------------------------------------------- } Dr Keith J. Morris } Molecular Cytogenetics and Microscopy Core } Laboratory 00/069 and 00/070 } The Wellcome Trust Centre for Human Genetics } Roosevelt Drive } Oxford } OX3 7BN } United Kingdom } Telephone: +44 (0)1865 287568 } Email: kjmorris-at-well.ox.ac.uk } Web-pages: http://www.well.ox.ac.uk/cytogenetics/ } } } } } } } } } } } } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: 14 May 2008 11:10 } To: kjmorris-at-well.ox.ac.uk } Subject: [Microscopy] Storage of organs at -°C } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } I have been asked to found the best method to freeze entire rat organs for } an undetermined period of time, maybe years. } They may be recovered any time for histology observation. I told the people } that it was probably better to leave them in a fixative at 4°C but they want } them frozen. } I am not very experienced in cryopreservation (except for cells in culture), } especially of whole organs. For me the ideal way would be to perfuse with } glycerol (I seem to remember something like 60%) but I simply wonder if we } master the technique to cryopreserve whole rat organs. Fact is, here we need } only to maintain the structure, not the activity! Problem is, the organs } cannot be perfused. They will be fixed in formalin and given to me whole. } If anyone has experience or infos to share, they are welcome. } Stephane } } P.S: don't laugh: they just wanted to quick-freeze them by directly plunging } the organs in liquid nitrogen! } } } } } } ==============================Original Headers============================== } 5, 21 -- From nizets2-at-yahoo.com Wed May 14 04:58:21 2008 } 5, 21 -- Received: from web37408.mail.mud.yahoo.com } (web37408.mail.mud.yahoo.com [209.191.91.140]) } 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } m4E9wJBu013979 } 5, 21 -- for {microscopy-at-microscopy.com} ; Wed, 14 May 2008 04:58:20 } -0500 } 5, 21 -- Received: (qmail 52964 invoked by uid 60001); 14 May 2008 09:58:18 } -0000 } 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 5, 21 -- s=s1024; d=yahoo.com; } 5, 21 -- } h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Ty } pe:Content-Transfer-Encoding:Message-ID; } 5, 21 -- } b=Ugb9vRZAp8URzwCI3wPnghgAYSea9wVL7Ns9i23nrZtzYMfvs8axWVV0CnBNS+Q7ujDZPm8ab9 } CLkBivhz8DOfRup4644oJHeVLiiWgPGecRP9Q1MdqHtTJn0t8Gej7IE31WJtYnsAiN+nqmx3uAF0 } mNTY6pPpiIWlw0qT1O5AM=; } 5, 21 -- X-YMail-OSG: } r.1ABgkVM1nSNshliAQjRtqrpjanTBdVflfVjg59iUVM4_mafkpP66FQpKMn7PN1HppsueOzhNCa } MjanTQLz.axgQShfpB2rJF2jZw-- } 5, 21 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via } HTTP; Wed, 14 May 2008 02:58:18 PDT } 5, 21 -- X-Mailer: YahooMailRC/975.23 YahooMailWebService/0.7.185 } 5, 21 -- Date: Wed, 14 May 2008 02:58:18 -0700 (PDT) } 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 5, 21 -- Subject: =?iso-8859-1?Q?Storage_of_organs_at_-=B0C?= } 5, 21 -- To: microscopy-at-microscopy.com } 5, 21 -- MIME-Version: 1.0 } 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 } 5, 21 -- Message-ID: {636315.52694.qm-at-web37408.mail.mud.yahoo.com} } 5, 21 -- Content-Transfer-Encoding: 8bit } 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4E9wJBu013979 } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 30, 21 -- From kjmorris-at-well.ox.ac.uk Mon May 19 08:11:14 2008 } 30, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 30, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4JDBDKf032247 } 30, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 19 May 2008 08:11:14 -0500 } 30, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 30, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 30, 21 -- id 1Jy58z-0003Ez-1W } 30, 21 -- for Microscopy-at-Microscopy.Com; Mon, 19 May 2008 14:11:13 +0100 } 30, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 30, 21 -- To: {Microscopy-at-Microscopy.Com} } 30, 21 -- Subject: } =?iso-8859-1?Q?FW:_=5BMicroscopy=5D_Storage_of_organs_at_-=B0C?= } 30, 21 -- Date: Mon, 19 May 2008 14:13:13 +0100 } 30, 21 -- Message-ID: {000f01c8b9b2$17c0fce0$b22c4381-at-CytoWhizz} } 30, 21 -- MIME-Version: 1.0 } 30, 21 -- Content-Type: text/plain; } 30, 21 -- charset="iso-8859-1" } 30, 21 -- X-Mailer: Microsoft Office Outlook 11 } 30, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 30, 21 -- Thread-Index: Aci5shefwPe18dhcQeemlYK3XQsFlA== } 30, 21 -- Content-Transfer-Encoding: 8bit } 30, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4JDBDKf032247 } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 24 -- From prvs=Rosemary.White=0187dd7cf-at-csiro.au Mon May 19 18:36:55 2008 13, 24 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37]) 13, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JNafeh030054 13, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 18:36:45 -0500 13, 24 -- X-IronPort-AV: E=Sophos;i="4.27,511,1204462800"; 13, 24 -- d="scan'208";a="163827938" 13, 24 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 13, 24 -- by vic-ironport-int.csiro.au with ESMTP; 20 May 2008 08:14:03 +1000 13, 24 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 13, 24 -- Tue, 20 May 2008 08:14:03 +1000 13, 24 -- User-Agent: Microsoft-Entourage/11.0.0.040405 13, 24 -- Date: Tue, 20 May 2008 08:19:14 +1000 13, 24 -- Subject: Re: [Microscopy] FW: [Microscopy] Storage of organs at 13, 24 -- -=?ISO-8859-1?B?sA==?=C 13, 24 -- From: Rosemary White {rosemary.white-at-csiro.au} 13, 24 -- To: {Microscopy-at-microscopy.com} 13, 24 -- Message-ID: {C4583A02.B6D9%rosemary.white-at-csiro.au} 13, 24 -- In-Reply-To: {200805191320.m4JDKPmu009751-at-ns.microscopy.com} 13, 24 -- Mime-version: 1.0 13, 24 -- Content-type: text/plain; 13, 24 -- charset="UTF-8" 13, 24 -- X-OriginalArrivalTime: 19 May 2008 22:14:03.0288 (UTC) FILETIME=[A5667980:01C8B9FD] 13, 24 -- Content-Transfer-Encoding: 8bit 13, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4JNafeh030054 ==============================End of - Headers==============================
Vlad is right, Molecular Biology grade water is a very expensive, highly specific product, and using it would be very much overkill.
As for your other problems, First: any water system may have scale build up. There were some very nice studies done in the 60's demonstrating scale. For the life of me I cannot remember any of the references, however. This is, in my humble opinion, one of the greatest problems we will ever face.
Second, the longer the water sits there, the greater the chance of some microorganism growing in it - even if you think there is nothing it can grow on. My memory is auxotrophs will grow in just plain water if they only have light as an energy source. May have the particular classification wrong, if so, some one will graciously provide the right classification.
Third, clean bottles are not necessarily clean. Our wash-up insists things come out clean - they don't. We make our own "molecular biology grade" water - it's prepared by treatment with DEPC. Had to throw out a whole batch because the student had not checked to ensure the bottle caps were clean. The all had a small amount of agarose dried on the lids. It had come off and contaminated the water during the preparation procedures. Moral of the story - Check and clean your bottles yourself.
I have used distilled reverse osmosis water - belive it is the same as your nanopure - for EM for the last 20+ years, and before that it was double distilled water. It can work well, but you have to apply some precaution. In our case, I insist on some overkill. First, check the bottles to ensure they are clean - including the lids. Second, filter fresh distilled R/O (or other) water into the bottles using a 0.2micron bottle top filter. Should remove just about anything, including most spores. Third, autoclave the water after filtering - as I said, it is overkill. Fourth, pour out your water from the bottle into a beaker, and discard what you don't use from the beaker. If you were careful the stock bottle should be OK. Do Not Try To save the water in the beaker for later use - it will usually work out, but remember Murphy, the one time it is critical, it will not work out. I usually prepare about 10L at a time, half in 500ml bottles, the rest in 100-250ml bottles.
I use the water in all steps, from preparing stains - positive or negative, for buffer stock and final solutions, for fixative preparations, for diluting, and for all water based rinses. Note, I also filter my stock concentrated buffer solutions when they are made, and the stains at each use.
Any Nanopure/RO or whatever water source should work well if you apply reasonable precautions, from just filtering through a 0.2 micron filter to the full preparation I insist on here.
Paul
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca {mailto:paul_hazelton-at-umanitoba.ca} Phone:204-789-3313 (w); 204-489-6924 (h) Cell:204-781-6982 Fax:204-789-392
==============================Original Headers============================== 14, 22 -- From paul_hazelton-at-umanitoba.ca Mon May 19 20:55:01 2008 14, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4K1t1NR003148 14, 22 -- for {microscopy-at-microscopy.com} ; Mon, 19 May 2008 20:55:01 -0500 14, 22 -- Received: from [192.168.100.102] (wnpgmb014vw-ad05-245-173.dynamic.mts.net [205.200.245.173]) 14, 22 -- (authenticated bits=0) 14, 22 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m4K1sqVa029791 14, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 14, 22 -- for {microscopy-at-microscopy.com} ; Mon, 19 May 2008 20:54:56 -0500 (CDT) 14, 22 -- Message-ID: {48322F70.7040900-at-umanitoba.ca} 14, 22 -- Date: Mon, 19 May 2008 20:54:56 -0500 14, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 14, 22 -- Organization: University of Manitoba 14, 22 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 14, 22 -- MIME-Version: 1.0 14, 22 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 14, 22 -- Subject: Re: [Microscopy] Fwd: viaWWW: "Pure" water?? 14, 22 -- References: {200805191835.m4JIZjag012450-at-ns.microscopy.com} 14, 22 -- In-Reply-To: {200805191835.m4JIZjag012450-at-ns.microscopy.com} 14, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 22 -- Content-Transfer-Encoding: 7bit 14, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
You say nothing about size and weight of your object, but if it's in the cm (or up to a few cm) range (and weight in relation), you could look at mechano-optical components manufacturer like Thor-Labs, Micro-Control, Physik-Intsrumente, etc. They have all single translation and rotation stages, which can be mounted together to build a 5 or 6 degrees of freedom stage. Something like a "lego" system !
An other option coud be to find an old Fedorov sample stage used in mineralogy for polarised light microscopy. They have most 3 rotations and two translations. These are not more manufactured, and can only be found, maybe with some diffculies, as second hand. But if there is a mineralogical lab in your neighbourhood, they may have one in a drawer.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
kim-at-kimvdlinde.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } I am looking for a stage that makes it possible to look at an object } from all sides, in other words, one that can be rotated along the three } possible axes independently. I have found one option, produced by } keyence, but I would like to find other solutions, preferably ones that } are more within the budget range that we have. Can anyone help? } } Kim }
==============================Original Headers============================== 9, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue May 20 07:51:31 2008 9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4KCpUqg013335 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 20 May 2008 07:51:30 -0500 9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m4KCpSBe037881 9, 29 -- for {microscopy-at-microscopy.com} ; Tue, 20 May 2008 14:51:28 +0200 (CEST) 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 570A13EC03B 9, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 20 May 2008 14:38:12 +0200 (CEST) 9, 29 -- Message-ID: {4832C634.8030006-at-ipcms.u-strasbg.fr} 9, 29 -- Date: Tue, 20 May 2008 14:38:12 +0200 9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 29 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080502) 9, 29 -- MIME-Version: 1.0 9, 29 -- To: Microscopy-at-microscopy.com 9, 29 -- Subject: Re: [Microscopy] Flexible stages with 6 degrees of freedom 9, 29 -- References: {200805192109.m4JL9QMY031273-at-ns.microscopy.com} 9, 29 -- In-Reply-To: {200805192109.m4JL9QMY031273-at-ns.microscopy.com} 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.154]); Tue, 20 May 2008 14:51:28 +0200 (CEST) 9, 29 -- X-Virus-Scanned: ClamAV 0.93/6668/Tue Apr 8 13:57:49 2008 on mr4.u-strasbg.fr 9, 29 -- X-Virus-Status: Clean 9, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=disabled 9, 29 -- version=3.2.4 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr4.u-strasbg.fr ==============================End of - Headers==============================
Thanks very much for the reply. My main aim, at least initially, is to use the AFM as a non-destructive characterization tool to locate and image nanomaterials, such as nanoparticles and nanowires, instead of an SEM for example that tends to leave contaminations and even damage from the electron beam. These materials will be mostly lying flat on the surface of a silicon dioxide chip with dimensions of about 5 mm at max. Initially, I just want to know the dimensions and locations of these materials, and I believe initially I and my students will be the only users of the instrument. But, would be nice if the system could be easily upgraded later on to perform more advanced functions, such as moving nanomaterials, lithography, etc. My total budget is about $200K (£100K?).
Thanks again.
Eddy
----- Start Original Message ----- Sent: Tue, 20 May 2008 08:49:51 +0100 X-from: John Mitchels {john.mitchels-at-bristol.ac.uk} To: ess26-at-runbox.com
Hi Jaci,
I don't know what your original material might be but I have tried many different filter papers for the purpose of slow and even wicking of grids and have found that a #40 Whatman has a nice rate.
Bob Underwood University of Washington
On Mon, 19 May 2008 LettJ-at-ent.wustl.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } I hope someone can point me in the right direction. } } We have a post-doc using our facility that has been searching for a } specific type of material to wick solutions from her specimen grids. } She says that in the past, she had used something that drew fluids away } slowly, such that her sample (specifically suspended particulates) would } safely "lower" onto the mesh (or film substrate) without being sucked } off the grid and absorbed by the wicking material. (Because of the } protocol she used, she couldn't wait each time for the solution to } evaporate.) She didn't think it was filter paper, and described it as } coming in rectangular strips and being just brittle enough that it would } "snap" if you bent it, leaving clean edges without loose fibers to } potentially contaminate the grids. She tried contacting the technician } that originally showed her this, but that person didn't know (or } couldn't remember) what the product was. } } I would also be interested in this product for similar reasons } (sometimes filter paper and dental paper points are much too fast and } evaporation is too slow). Does anyone have any suggestions? } } Thank you, } } Jaci } } Jaclynn Lett } Senior Research Technician, EM Facility } Research Center for Auditory and Vestibular Studies } Department of Otolaryngology } Washington University School of Medicine } 660 S. Euclid Ave., Campus Box 8115 } St. Louis, MO 63110 } } Voice: 314-747-7257 } Fax: 314-747-7230 } Email: lettj-at-ent.wustl.edu } http://otocore.wustl.edu } } {br/} The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. } } } ==============================Original Headers============================== } 10, 23 -- From LettJ-at-ent.wustl.edu Mon May 19 15:37:23 2008 } 10, 23 -- Received: from MAIL3.wusm-pcf.wustl.edu (mail3.wusm-pcf.wustl.edu [128.252.17.170]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4JKbN1u028867 } 10, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 19 May 2008 15:37:23 -0500 } 10, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL3.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.3959); } 10, 23 -- Mon, 19 May 2008 15:36:35 -0500 } 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 10, 23 -- Content-class: urn:content-classes:message } 10, 23 -- MIME-Version: 1.0 } 10, 23 -- Content-Type: text/plain; } 10, 23 -- charset="us-ascii" } 10, 23 -- Subject: TEM: wicking or blotting grids } 10, 23 -- Date: Mon, 19 May 2008 15:37:22 -0500 } 10, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C390B1B7F-at-EX04.wusm-pcf.wustl.edu} } 10, 23 -- X-MS-Has-Attach: } 10, 23 -- X-MS-TNEF-Correlator: } 10, 23 -- Thread-Topic: TEM: wicking or blotting grids } 10, 23 -- Thread-Index: Aci58B/0iRZZmd/WS+GoUZ5OhYbycQ== } 10, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} } 10, 23 -- To: {Microscopy-at-microscopy.com} } 10, 23 -- X-OriginalArrivalTime: 19 May 2008 20:36:35.0037 (UTC) FILETIME=[079230D0:01C8B9F0] } 10, 23 -- Content-Transfer-Encoding: 8bit } 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4JKbN1u028867 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 22 -- From underwoo-at-u.washington.edu Tue May 20 10:02:08 2008 9, 22 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4KF28Y9011423 9, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 20 May 2008 10:02:08 -0500 9, 22 -- Received: from hymn13.u.washington.edu (hymn13.u.washington.edu [140.142.4.103]) 9, 22 -- by mxout2.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m4KF27bI025307 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 9, 22 -- Tue, 20 May 2008 08:02:07 -0700 9, 22 -- Received: from localhost (localhost [127.0.0.1]) 9, 22 -- by hymn13.u.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m4KF27ZE030410; 9, 22 -- Tue, 20 May 2008 08:02:07 -0700 9, 22 -- X-Auth-Received: from [128.208.106.143] by hymn13.u.washington.edu via HTTP; Tue, 20 May 2008 08:02:07 PDT 9, 22 -- Date: Tue, 20 May 2008 08:02:07 -0700 (PDT) 9, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 9, 22 -- To: LettJ-at-ent.wustl.edu, Microscopy List {Microscopy-at-MSA.Microscopy.Com} 9, 22 -- Subject: Re: [Microscopy] TEM: wicking or blotting grids 9, 22 -- In-Reply-To: {200805192038.m4JKcogH030680-at-ns.microscopy.com} 9, 22 -- Message-ID: {Pine.LNX.4.43.0805200802070.14041-at-hymn13.u.washington.edu} 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 9, 22 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.20.74647 9, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_NOT_1 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' ==============================End of - Headers==============================
Thanks for the reply. Congrads for your new AFM! I am mostly interested in AFMming nanomaterials, such as nanoparticles and nanowires for research purposes at a university lab. Initially to non-destructively measure their dimensions and positions. So basically I am looking for something good and reliable, but within a reasonable budget (I have $200K to spend). Also, would be nice if the system could be upgraded to more functions later on without spending a fortune on it. As suggested, I will look into Veeco and the Asylum Research systems (cost?). Are the inverted optical microscope in AFMs used to locate samples? Or have other functions? In other words, are they a must in an AFM?
Thanks again.
Eddy
P.S. Appreciate it if you could kindly send me a PM about the prices you found from your research. Thank you.
----- Start Original Message ----- Sent: Tue, 20 May 2008 07:12:33 -0500 X-from: "Craig Anderson Ext 1365" {Craig.Anderson-at-saintpaul.edu} To: {ess26-at-runbox.com}
You, your colleagues and your students are invited to the Bio-Inspired Design - Enabling Technologies in the Life and Materials Sciences Conference to be held at Mississippi State University (MSU), August 20-22, 2008.
This conference is being funded by MSU in response to a growing interdisciplinary effort focused toward the advancement of technology beyond current capabilities and to bridge Life and Materials Sciences through Bio-Inspired Designs. Several MSU core facilities contributing to this interdisciplinary enterprise include the Center for Advanced Vehicular Systems, the Electron Microscope Center, and the Facility for Organismal and Cellular Imaging. This effort seeks to 1) attract regional and National interests working at the interface between Life and Materials Sciences, 2) involve students, faculty, corporate partners and industry in these interdisciplinary efforts, and 3) serve as a forum for the ex-change of knowledge through solicitations for abstracts and presentations coupled with open information exchange groups and discussion panels to round out a dynamic program.
We have a keynote speaker list that includes recognized leaders in the Life and Materials Sciences. Speakers were chosen because each has a cross-disciplinary focus that transcends the *Bio-Inspired Design* concept. We will also have a series of demos/tutorials/workshops sponsored by corporate partners and focused on sharing state-of-the-art materials and life science technologies. We believe this symposium will act as a catalyst for on-campus, regional, and national initiatives in this important area. Our vision for conference outcomes is to facilitate conversation and idea exchange between the life scientists and engineers from diverse areas.
We ask that you share this information widely and encourage participation by engineers, architects, life scientists and any others interested in Bio-Inspiration in the broad sense. Please go to www.bioinspired.msstate.edu for more information on the keynote speakers, program, and registration. We will waive all registration fees for students. A key contact will be Anna Chromiak (achromiak-at-ads.msstate.edu) if you have any questions regarding logistics.
Please give this invitation your positive consideration and distribute it to your faculty/colleagues/students and industry partners. We look forward to hearing from you and having your participation at the BioInspired Design conference, August 20-22.
Sincerely, Giselle Thibaudeau, Director Electron Microscope Center, giselle-at-emcenter.msstate.edu Randall German, Center for Advanced Vehicular Systems Director, german-at-cavs.msstate.edu Scott Willard, co-Director Facility for Organismal and Cellular Imaging, swillard-at-ads.msstate.edu
==============================Original Headers============================== 8, 18 -- From ALawrence-at-entomology.msstate.edu Tue May 20 16:27:26 2008 8, 18 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4KLRQ6T021768 8, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 20 May 2008 16:27:26 -0500 8, 18 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 8, 18 -- with Novell_GroupWise; Tue, 20 May 2008 16:27:25 -0500 8, 18 -- Message-Id: {4832FBE7.B26A.00E6.0-at-entomology.msstate.edu} 8, 18 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 8, 18 -- Date: Tue, 20 May 2008 16:27:19 -0500 8, 18 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 8, 18 -- To: {Microscopy-at-Microscopy.Com} 8, 18 -- Subject: BioInspired Design Conference 8, 18 -- References: {4832F9D1020000E60003E88B-at-mailhost2.groupwise.msstate.edu} 8, 18 -- {4832FBE7020000E60003E898-at-mailhost2.groupwise.msstate.edu} 8, 18 -- Mime-Version: 1.0 8, 18 -- Content-Type: text/plain; charset=ISO-8859-15 8, 18 -- Content-Transfer-Encoding: 8bit 8, 18 -- Content-Disposition: inline ==============================End of - Headers==============================
Thanks for the feedback. I can see that there is some kind of consensus from your reply and the others on the advantages of Asylum's AFM for research. I will see if my budget allow me to get a deal from them. Otherwise, Veeco seems also a decent choice with improving their customer support and service (hope they are reading this!).
Thanks a lot to everybody for your replies.
Eddy
----- Start Original Message ----- Sent: Tue, 20 May 2008 09:59:39 -0500 X-from: "Scott Maclaren" {maclaren-at-mrl.uiuc.edu} To: {ess26-at-runbox.com}
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==============================Original Headers============================== 8, 29 -- From Resume-at-fei.com Wed May 21 10:53:16 2008 8, 29 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LFrGOb008649 8, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 21 May 2008 10:53:16 -0500 8, 29 -- X-WSS-ID: 0K186ST-01-K08-01 8, 29 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 8, 29 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 27FE983651D 8, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 21 May 2008 08:53:16 -0700 (PDT) 8, 29 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 8, 29 -- Wed, 21 May 2008 08:53:14 -0700 8, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 29 -- Content-class: urn:content-classes:message 8, 29 -- MIME-Version: 1.0 8, 29 -- Content-Type: text/plain; 8, 29 -- charset="us-ascii" 8, 29 -- Subject: RE: Welcome to the Microscopy ListServer:Info,Rules & FAQ 8, 29 -- Date: Wed, 21 May 2008 08:53:14 -0700 8, 29 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB81174CCA5-at-hlexc03.w2k.feico.com} 8, 29 -- In-Reply-To: {200805211309.m4LD9DlI030485-at-ns.microscopy.com} 8, 29 -- X-MS-Has-Attach: 8, 29 -- X-MS-TNEF-Correlator: 8, 29 -- Thread-Topic: Welcome to the Microscopy ListServer:Info,Rules & FAQ 8, 29 -- Thread-Index: Aci7Q+Om8EF2if5GQiSeRUDOKBGDawAFrVBA 8, 29 -- References: {200805211309.m4LD9DlI030485-at-ns.microscopy.com} 8, 29 -- From: "Resume" {Resume-at-fei.com} 8, 29 -- To: {Microscopy-at-Microscopy.Com} 8, 29 -- X-OriginalArrivalTime: 21 May 2008 15:53:14.0957 (UTC) FILETIME=[C78F57D0:01C8BB5A] 8, 29 -- Content-Transfer-Encoding: 8bit 8, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4LFrGOb008649 ==============================End of - Headers==============================
I am interested in liquid suspension/dispersing techniques that will allow me to disperse small particles (10 to 0.1 microns in size) onto an SEM substrate so the individual particles are well separated (i.e. no clumping/flocculating etc.). While I suspect this is a well known problem in SEM sample prep, maybe with some known techniques, I've been trying things without any outside help so far.
I've had some success using ethanol with a few drops of a high quality commercial surfactant, but after the ethanol evaporates the oily surfactant is left as a coating on the particles, which makes SEM impractical.
The material I'm working with are small inorganic (mineral) particles. But the material aside, I'm wondering in particular if the life science side of the EM house has some suggestions that might help.
Thanks,
Roy Christoffersen SAIC FE-STEM Facility Manager, ARES Directorate NASA Johnson Space Center 281-244-8380
==============================Original Headers============================== 7, 27 -- From roy.christoffersen-1-at-nasa.gov Wed May 21 11:45:59 2008 7, 27 -- Received: from ndjsbar02.ndc.nasa.gov (ndjsbar02.ndc.nasa.gov [198.120.25.39]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LGjwv5024756 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:45:58 -0500 7, 27 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) 7, 27 -- by ndjsbar02.ndc.nasa.gov (Spam Firewall) with ESMTP id C235D472564 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:45:53 -0500 (CDT) 7, 27 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) by ndjsbar02.ndc.nasa.gov with ESMTP id aLulHLOJZrnR26Xi for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:45:53 -0500 (CDT) 7, 27 -- Received: from NDJSEVS22A.ndc.nasa.gov ([129.166.32.220]) by ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Wed, 21 May 2008 11:45:53 -0500 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- Subject: SEM sample prep: particle dispersions 7, 27 -- Date: Wed, 21 May 2008 11:45:53 -0500 7, 27 -- Message-ID: {0E899C0EBD04EF4FA965E0D375B2D4FB02448E84-at-NDJSEVS22A.ndc.nasa.gov} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: SEM sample prep: particle dispersions 7, 27 -- thread-index: Aci7YiHnd48XabcEQlK/URpfNE9w2Q== 7, 27 -- From: "Christoffersen, Roy (JSC-KR)[SAIC]" {roy.christoffersen-1-at-nasa.gov} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 21 May 2008 16:45:53.0872 (UTC) FILETIME=[226B8500:01C8BB62] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4LGjwv5024756 ==============================End of - Headers==============================
On May 21, 2008, at 9:46 AM, roy.christoffersen-1-at-nasa.gov wrote:
} I am interested in liquid suspension/dispersing techniques that will } allow me to disperse small particles (10 to 0.1 microns in size) } onto an } SEM substrate so the individual particles are well separated (i.e. no } clumping/flocculating etc.). While I suspect this is a well known } problem in SEM sample prep, maybe with some known techniques, I've } been } trying things without any outside help so far. } } I've had some success using ethanol with a few drops of a high quality } commercial surfactant, but after the ethanol evaporates the oily } surfactant is left as a coating on the particles, which makes SEM } impractical. } } The material I'm working with are small inorganic (mineral) particles. } But the material aside, I'm wondering in particular if the life } science } side of the EM house has some suggestions that might help.
Dear Roy, The closest I come to a problem like yours is putting 10 nm Au particles on cryoTEM grids. They have a tendency to aggregate, and I want them well-dispersed, so I try to evaporate the water they're suspended in rapidly. I have found that putting the grids in a 50-70 degree oven does the job. You can also try to taylor the properties of your SEM substrate. If the particles bind to the substrate, then they should have less tendency to clump. Another thought is to use an atomizer to deposit small drops of your suspension onto the substrate. If the substrate can be kept warm, the drops will evaporate quickly, so the particles should be in fixed positions, more or less, and this should give you a good distribution. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed May 21 12:23:12 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LHNCxA007354 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 21 May 2008 12:23:12 -0500 6, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 6, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id C792B13E8D 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 21 May 2008 10:23:10 -0700 (PDT) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 0F05813E88 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 21 May 2008 10:23:06 -0700 (PDT) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200805211646.m4LGk98v024890-at-ns.microscopy.com} 6, 22 -- References: {200805211646.m4LGk98v024890-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {BC33C8A6-E94E-41E4-AC8B-26559BA1B84A-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] SEM sample prep: particle dispersions 6, 22 -- Date: Wed, 21 May 2008 10:23:14 -0700 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Chuck Garber described a technique for separating particles in an email to the listserver some time ago which I've attached below. I don't know if it will help in your situation, but it is probably worth looking at.
Cheers, Henk Colijn
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Oldrich Benada wrote: ===================================================== I need some advice. I was asked to do some analysis of silica particles (size distribution) for chemist in our institute. Particle size should be in the range of 3 to 6 um. I do not have any experiences with such sample. Could someone give me a tip how to prepare sample for TEM (or SEM)? ====================================================== The problem is that those pesky silica particles don't know that they are supposed to separate and stay away from each other when dispersed in a liquid followed by a droplet of this liquid suspension being placed on a solid surface. They tend to agglomerate very quickly leading to a difficult- to-analyze situation, especially using automated means of analysis. You are correct in that the size range expected could be on the order of 3-6 nm.
This is the ideal application for the camphor/naphthalene method which I described several years ago. Credit for the technique, or at least the one who taught it to me was an innovative microscopist then working at the DuPont Experimental Station in Wilmington, DE by the name of Robert P. Schatz, in about 1968, now deceased. Take a 60% camphor/40% naphthalene mixture and heat it to twenty or so degrees above room temperature on a hot plate in a small beaker or flask, the two organics are miscible in each other and this is the eutectic composition.
Once a clear liquid, add a small amount of the silica (not more than 0.1%), which disperses quite readily. Then, using a pipette, take out some liquid and put a drop onto a carbon coated glass slide, at which time the drop is instantly frozen solid (it is at room temperature). Put the slide into your vacuum evaporator to pump out all night, and the "magic" is that the solid eutectic sublimes at room temperature at a rate that by morning, it is completely gone, leaving the silica particles uniformly dispersed on the carbon film!
The rest is obvious. You can pick this up on a grid, as is, or in order to bring out more contrast, Pt/C shadow, probably using an angle not more than 30 degrees. You can float the "replica" off of the slide directly onto a grid and viola! you have particles completely dispersed, virtually no doublets or triplets, and a field quite amenable for automated image analysis (as a bonus).
One important further suggestion: Some times these silica particles tend to fuse together as little "chains". If you suspect this is happening, be sure to take the micrographs as stereo pairs because you can in fact capture this three dimensional spatial information.
Disclaimer: We do not sell either the camphor or naphthalene so have no vested interest in whether people use this method or not. It is just a really neat method for the preparation of fine particle samples in this size range. We are obviously set up to use this method as a service for others, however.
Chuck
At 12:47 PM 05/21/08, roy.christoffersen-1-at-nasa.gov wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 17, 26 -- From colijn.1-at-osu.edu Wed May 21 12:43:09 2008 17, 26 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LHh9BL020906 17, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 12:43:09 -0500 17, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 17, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31224) 17, 26 -- id {01MV1W9GZJSG8XTTTH-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 17, 26 -- Wed, 21 May 2008 13:43:08 -0400 (EDT) 17, 26 -- Received: from HOC1.ecr6.ohio-state.edu 17, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 17, 26 -- (PMDF V6.3-x13 #31224) 17, 26 -- with ESMTPA id {01MV1W9GNFPY8XS3BW-at-ecr6.ohio-state.edu} ; Wed, 17, 26 -- 21 May 2008 13:43:08 -0400 (EDT) 17, 26 -- Date: Wed, 21 May 2008 13:42:19 -0400 17, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 17, 26 -- Subject: Re: [Microscopy] SEM sample prep: particle dispersions 17, 26 -- In-reply-to: {200805211647.m4LGlT9X027152-at-ns.microscopy.com} 17, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 17, 26 -- To: roy.christoffersen-1-at-nasa.gov 17, 26 -- Cc: microscopy-at-microscopy.com 17, 26 -- Message-id: {7.0.1.0.2.20080521133825.035b2bf8-at-osu.edu} 17, 26 -- MIME-version: 1.0 17, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 17, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 17, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 17, 26 -- References: {200805211647.m4LGlT9X027152-at-ns.microscopy.com} ==============================End of - Headers==============================
Vascular density is a type of length density. Length density can be determined using stereology. There are several stereological methods that can be used to estimate length density. An excellent software packagage available for length density estimation is newCAST by Visiopharm.
The best method for length or length density estimation in a flat mounted material is to use a line probe. Probes are geometrical shapes which are used to explore geometrical quantities. The intersection between the vessels and the lines can be used to estimate length.
The method used can be found in a number of commonly available books including Underwood, Weibel, DeHoff, and Russ. Most books on the subject will have some information on length estimation.
Stereologists rarely use densities. Absolute values are better to use. Densities are often misleading. The problems with densities are described as "reference trap" issues.
Quoting eunice.cheung-at-regeneron.com:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both eunice.cheung-at-regeneron.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: eunice.cheung-at-regeneron.com } Name: Eunice Cheung } } Organization: Regeneron Pharmaceuticals } } Title-Subject: [Filtered] Calculating Vascular Density and Length } } Question: I am studying vascular network in a in vivo flatmounted } diseased mouse model. With fluorescence, it easy to see that a } difference in vascular density between the diseased mouse and the } normal mouse. What is the best software and approach to measure } vessel length and density? } -Eunice } } Login Host: 66.150.82.115 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Fri May 16 19:14:16 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4H0EFbL010993 } 6, 11 -- for {microscopy-at-microscopy.com} ; Fri, 16 May 2008 19:14:16 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240803c453d3c85496-at-[206.69.208.22]} } 6, 11 -- Date: Fri, 16 May 2008 19:14:14 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: eunice.cheung-at-regeneron.com (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Calculating Vascular Density and Length } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 8, 30 -- From rboehrin-at-vt.edu Wed May 21 13:01:22 2008 8, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LI1L86001861 8, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2008 13:01:22 -0500 8, 30 -- Received: from vivi.cc.vt.edu (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) 8, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id m4LI1LL1017075 8, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2008 14:01:21 -0400 8, 30 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 8, 30 -- by vivi.cc.vt.edu (MOS 3.8.6-GA) 8, 30 -- with ESMTP id JKL64058; 8, 30 -- Wed, 21 May 2008 14:01:21 -0400 (EDT) 8, 30 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain [127.0.0.1]) 8, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id m4LI1K0K009556 8, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2008 14:01:21 -0400 8, 30 -- Received: (from apache-at-localhost) 8, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id m4LI1K4C009555 8, 30 -- for Microscopy-at-microscopy.com; Wed, 21 May 2008 14:01:20 -0400 8, 30 -- Received: from us194px00-pat.tycoelectronics.net (us194px00-pat.tycoelectronics.net [198.175.154.212]) 8, 30 -- by webmail.vt.edu (IMP) with HTTP; Wed, 21 May 2008 14:01:20 -0400 8, 30 -- Message-ID: {1211392880.48346370cea33-at-webmail.vt.edu} 8, 30 -- Date: Wed, 21 May 2008 14:01:20 -0400 8, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} 8, 30 -- To: Microscopy-at-microscopy.com 8, 30 -- Subject: Re: [Microscopy] viaWWW: Calculating Vascular Density and Length 8, 30 -- References: {200805170027.m4H0RQJb017351-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {200805170027.m4H0RQJb017351-at-ns.microscopy.com} 8, 30 -- MIME-Version: 1.0 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
Dear Roy, I would make sure that your substrate is very flat: a glass cover slip or vitreous carbon. Suspend your particles in pure alcohol and sonicate for a minute, don't use the surfactant for the reasons you mention. Put one drop of your suspension onto the substrate and it should spread out quite well. The alcohol should evaporate quite quickly, but particles at that size range should not agglomerate too badly. You should be able to find good areas on your sample to examine. Regards,
-----Original Message----- X-from: roy.christoffersen-1-at-nasa.gov [mailto:roy.christoffersen-1-at-nasa.gov] Sent: May 21, 2008 9:53 AM To: maryflet-at-interchange.ubc.ca
I am interested in liquid suspension/dispersing techniques that will allow me to disperse small particles (10 to 0.1 microns in size) onto an SEM substrate so the individual particles are well separated (i.e. no clumping/flocculating etc.). While I suspect this is a well known problem in SEM sample prep, maybe with some known techniques, I've been trying things without any outside help so far.
I've had some success using ethanol with a few drops of a high quality commercial surfactant, but after the ethanol evaporates the oily surfactant is left as a coating on the particles, which makes SEM impractical.
The material I'm working with are small inorganic (mineral) particles. But the material aside, I'm wondering in particular if the life science side of the EM house has some suggestions that might help.
Thanks,
Roy Christoffersen SAIC FE-STEM Facility Manager, ARES Directorate NASA Johnson Space Center 281-244-8380
==============================Original Headers============================== 7, 27 -- From roy.christoffersen-1-at-nasa.gov Wed May 21 11:45:59 2008 7, 27 -- Received: from ndjsbar02.ndc.nasa.gov (ndjsbar02.ndc.nasa.gov [198.120.25.39]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LGjwv5024756 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:45:58 -0500 7, 27 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) 7, 27 -- by ndjsbar02.ndc.nasa.gov (Spam Firewall) with ESMTP id C235D472564 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:45:53 -0500 (CDT) 7, 27 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) by ndjsbar02.ndc.nasa.gov with ESMTP id aLulHLOJZrnR26Xi for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:45:53 -0500 (CDT) 7, 27 -- Received: from NDJSEVS22A.ndc.nasa.gov ([129.166.32.220]) by ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Wed, 21 May 2008 11:45:53 -0500 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- Subject: SEM sample prep: particle dispersions 7, 27 -- Date: Wed, 21 May 2008 11:45:53 -0500 7, 27 -- Message-ID: {0E899C0EBD04EF4FA965E0D375B2D4FB02448E84-at-NDJSEVS22A.ndc.nasa.gov} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: SEM sample prep: particle dispersions 7, 27 -- thread-index: Aci7YiHnd48XabcEQlK/URpfNE9w2Q== 7, 27 -- From: "Christoffersen, Roy (JSC-KR)[SAIC]" {roy.christoffersen-1-at-nasa.gov} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 21 May 2008 16:45:53.0872 (UTC) FILETIME=[226B8500:01C8BB62] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4LGjwv5024756 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 35 -- From maryflet-at-interchange.ubc.ca Wed May 21 13:42:51 2008 16, 35 -- Received: from mr2.mail-relay.ubc.ca (mr2.mail-relay.ubc.ca [137.82.45.3]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LIgpEm016879 16, 35 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 13:42:51 -0500 16, 35 -- X-Ubc-Received: from mr2.mail-relay.ubc.ca (localhost [127.0.0.1]) 16, 35 -- by localhost (Postfix) with SMTP id 1C9C9C771 16, 35 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:42:51 -0700 (PDT) 16, 35 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 16, 35 -- by mr2.mail-relay.ubc.ca (Postfix) with ESMTP 16, 35 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 11:42:50 -0700 (PDT) 16, 35 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 16, 35 -- by smtp.interchange.ubc.ca 16, 35 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 16, 35 -- with ESMTP id {0K1800GWBENCZZ-at-smtp.interchange.ubc.ca} for 16, 35 -- microscopy-at-microscopy.com; Wed, 21 May 2008 11:42:49 -0700 (PDT) 16, 35 -- Date: Wed, 21 May 2008 11:42:18 -0700 16, 35 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 16, 35 -- Subject: RE: [Microscopy] SEM sample prep: particle dispersions 16, 35 -- In-reply-to: {200805211653.m4LGr6Jb002628-at-ns.microscopy.com} 16, 35 -- To: roy.christoffersen-1-at-nasa.gov 16, 35 -- Cc: microscopy-at-microscopy.com 16, 35 -- Reply-to: maryflet-at-interchange.ubc.ca 16, 35 -- Message-id: {0K1800GWCENCZZ-at-smtp.interchange.ubc.ca} 16, 35 -- Organization: Materials Eng. 16, 35 -- MIME-version: 1.0 16, 35 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 35 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 35 -- Content-type: text/plain; charset=us-ascii 16, 35 -- Content-transfer-encoding: 7bit 16, 35 -- Thread-index: Aci7YyUuUvRArpjCTrKt+QzmDHmQ7wADp2Fg 16, 35 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.21.112506 16, 35 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 16, 35 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_2_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0 16, 35 -- X-Spam-Level: 16, 35 -- X-Spam-Flag: No ==============================End of - Headers==============================
FYI - The published version of the camphor-naphthalene article is:
Thaulow, N.* and E. W White: General method for dispersing and disaggregating particulate samples for quantitative SEM and optical microscopic studies, Powder Tech, 5, 6, 377-379, 1972.
*Material Research Laboratory. Penn State Univ.
Tony
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-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Wednesday, May 21, 2008 1:47 PM To: ph2-at-sprynet.com
Hi Roy,
Chuck Garber described a technique for separating particles in an email to the listserver some time ago which I've attached below. I don't know if it will help in your situation, but it is probably worth looking at.
Cheers, Henk Colijn
-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
Oldrich Benada wrote: ===================================================== I need some advice. I was asked to do some analysis of silica particles (size distribution) for chemist in our institute. Particle size should be in the range of 3 to 6 um. I do not have any experiences with such sample. Could someone give me a tip how to prepare sample for TEM (or SEM)? ====================================================== The problem is that those pesky silica particles don't know that they are supposed to separate and stay away from each other when dispersed in a liquid followed by a droplet of this liquid suspension being placed on a solid surface. They tend to agglomerate very quickly leading to a difficult- to-analyze situation, especially using automated means of analysis. You are correct in that the size range expected could be on the order of 3-6 nm.
This is the ideal application for the camphor/naphthalene method which I described several years ago. Credit for the technique, or at least the one who taught it to me was an innovative microscopist then working at the DuPont Experimental Station in Wilmington, DE by the name of Robert P. Schatz, in about 1968, now deceased. Take a 60% camphor/40% naphthalene mixture and heat it to twenty or so degrees above room temperature on a hot plate in a small beaker or flask, the two organics are miscible in each other and this is the eutectic composition.
Once a clear liquid, add a small amount of the silica (not more than 0.1%), which disperses quite readily. Then, using a pipette, take out some liquid and put a drop onto a carbon coated glass slide, at which time the drop is instantly frozen solid (it is at room temperature). Put the slide into your vacuum evaporator to pump out all night, and the "magic" is that the solid eutectic sublimes at room temperature at a rate that by morning, it is completely gone, leaving the silica particles uniformly dispersed on the carbon film!
The rest is obvious. You can pick this up on a grid, as is, or in order to bring out more contrast, Pt/C shadow, probably using an angle not more than 30 degrees. You can float the "replica" off of the slide directly onto a grid and viola! you have particles completely dispersed, virtually no doublets or triplets, and a field quite amenable for automated image analysis (as a bonus).
One important further suggestion: Some times these silica particles tend to fuse together as little "chains". If you suspect this is happening, be sure to take the micrographs as stereo pairs because you can in fact capture this three dimensional spatial information.
Disclaimer: We do not sell either the camphor or naphthalene so have no vested interest in whether people use this method or not. It is just a really neat method for the preparation of fine particle samples in this size range. We are obviously set up to use this method as a service for others, however.
Chuck
At 12:47 PM 05/21/08, roy.christoffersen-1-at-nasa.gov wrote:
} --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 17, 26 -- From colijn.1-at-osu.edu Wed May 21 12:43:09 2008 17, 26 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LHh9BL020906 17, 26 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 12:43:09 -0500 17, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 17, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31224) 17, 26 -- id {01MV1W9GZJSG8XTTTH-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 17, 26 -- Wed, 21 May 2008 13:43:08 -0400 (EDT) 17, 26 -- Received: from HOC1.ecr6.ohio-state.edu 17, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 17, 26 -- (PMDF V6.3-x13 #31224) 17, 26 -- with ESMTPA id {01MV1W9GNFPY8XS3BW-at-ecr6.ohio-state.edu} ; Wed, 17, 26 -- 21 May 2008 13:43:08 -0400 (EDT) 17, 26 -- Date: Wed, 21 May 2008 13:42:19 -0400 17, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 17, 26 -- Subject: Re: [Microscopy] SEM sample prep: particle dispersions 17, 26 -- In-reply-to: {200805211647.m4LGlT9X027152-at-ns.microscopy.com} 17, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 17, 26 -- To: roy.christoffersen-1-at-nasa.gov 17, 26 -- Cc: microscopy-at-microscopy.com 17, 26 -- Message-id: {7.0.1.0.2.20080521133825.035b2bf8-at-osu.edu} 17, 26 -- MIME-version: 1.0 17, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 17, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 17, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 17, 26 -- References: {200805211647.m4LGlT9X027152-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 32, 28 -- From ph2-at-sprynet.com Wed May 21 13:45:58 2008 32, 28 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 32, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LIjtJQ021437 32, 28 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 13:45:57 -0500 32, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 32, 28 -- s=dk20050327; d=sprynet.com; 32, 28 -- b=aFJRTfOobFkyILdgyPKMYdLcpWPlMkDmCdc6CGXpkVyU2zmPxAXqCP89RXiu7fou; 32, 28 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:In-Reply-To:Message-ID:X-ELNK-Trace:X-Originating-IP; 32, 28 -- Received: from [75.61.18.94] (helo=user915fa8f284) 32, 28 -- by elasmtp-mealy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 32, 28 -- (envelope-from {ph2-at-sprynet.com} ) 32, 28 -- id 1JytJx-0002iy-7c 32, 28 -- for microscopy-at-microscopy.com; Wed, 21 May 2008 14:45:54 -0400 32, 28 -- From: "Tony Havics" {ph2-at-sprynet.com} 32, 28 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 32, 28 -- Subject: RE: [Microscopy] Re: SEM sample prep: particle dispersions 32, 28 -- Date: Wed, 21 May 2008 14:45:39 -0400 32, 28 -- MIME-Version: 1.0 32, 28 -- Content-Type: text/plain; 32, 28 -- charset="us-ascii" 32, 28 -- Content-Transfer-Encoding: 7bit 32, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 32, 28 -- Thread-Index: Aci7aqNCeb6oLVazQg634oagiuCTHgAB5WAw 32, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 32, 28 -- In-Reply-To: {200805211746.m4LHkj5e028539-at-ns.microscopy.com} 32, 28 -- Message-ID: {E1JytJx-0002iy-7c-at-elasmtp-mealy.atl.sa.earthlink.net} 32, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9853f9864a1a2bbf42360ac3dcc576bbc350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 32, 28 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
Another way to disperse particles dry is by simply pouring slowly into the top of a long tube or pipe held vertically over your substrate. The pipe should be something like 3-4 feet long and 2-3 inches in diameter. Just hold it vertically over an adhesive substrate. The fall through the still air in the tube breaks up the particles and reduces aglomeration.
John Mardinly, Numonyx
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Wednesday, May 21, 2008 10:32 AM To: MARDINLY, A
On May 21, 2008, at 9:46 AM, roy.christoffersen-1-at-nasa.gov wrote:
} I am interested in liquid suspension/dispersing techniques that will } allow me to disperse small particles (10 to 0.1 microns in size) } onto an } SEM substrate so the individual particles are well separated (i.e. no } clumping/flocculating etc.). While I suspect this is a well known } problem in SEM sample prep, maybe with some known techniques, I've } been } trying things without any outside help so far. } } I've had some success using ethanol with a few drops of a high quality } commercial surfactant, but after the ethanol evaporates the oily } surfactant is left as a coating on the particles, which makes SEM } impractical. } } The material I'm working with are small inorganic (mineral) particles. } But the material aside, I'm wondering in particular if the life } science } side of the EM house has some suggestions that might help.
Dear Roy, The closest I come to a problem like yours is putting 10 nm Au particles on cryoTEM grids. They have a tendency to aggregate, and I want them well-dispersed, so I try to evaporate the water they're suspended in rapidly. I have found that putting the grids in a 50-70 degree oven does the job. You can also try to taylor the properties of your SEM substrate. If the particles bind to the substrate, then they should have less tendency to clump. Another thought is to use an atomizer to deposit small drops of your suspension onto the substrate. If the substrate can be kept warm, the drops will evaporate quickly, so the particles should be in fixed positions, more or less, and this should give you a good distribution. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed May 21 12:23:12 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LHNCxA007354 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 21 May 2008 12:23:12 -0500 6, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 6, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id C792B13E8D 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 21 May 2008 10:23:10 -0700 (PDT) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id 0F05813E88 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 21 May 2008 10:23:06 -0700 (PDT) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200805211646.m4LGk98v024890-at-ns.microscopy.com} 6, 22 -- References: {200805211646.m4LGk98v024890-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {BC33C8A6-E94E-41E4-AC8B-26559BA1B84A-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] SEM sample prep: particle dispersions 6, 22 -- Date: Wed, 21 May 2008 10:23:14 -0700 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 28 -- From A.MARDINLY-at-numonyx.com Wed May 21 14:13:11 2008 15, 28 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LJDB8h011095 15, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 21 May 2008 14:13:11 -0500 15, 28 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23]) 15, 28 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id E2AAB1E8227 15, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 21 May 2008 13:38:46 -0400 (EDT) 15, 28 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 15, 28 -- Wed, 21 May 2008 15:13:10 -0400 15, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 28 -- Content-class: urn:content-classes:message 15, 28 -- MIME-Version: 1.0 15, 28 -- Content-Type: text/plain; 15, 28 -- charset="us-ascii" 15, 28 -- Subject: RE: [Microscopy] Re: SEM sample prep: particle dispersions 15, 28 -- Date: Wed, 21 May 2008 15:13:09 -0400 15, 28 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF93E86F1-at-EXDRESBENMX012.numonyx.local} 15, 28 -- In-Reply-To: {200805211732.m4LHWQWa020164-at-ns.microscopy.com} 15, 28 -- X-MS-Has-Attach: 15, 28 -- X-MS-TNEF-Correlator: 15, 28 -- Thread-Topic: [Microscopy] Re: SEM sample prep: particle dispersions 15, 28 -- Thread-Index: Aci7aLghcxSt6+Y6T8isZilcaaO3aAADXT1A 15, 28 -- References: {200805211732.m4LHWQWa020164-at-ns.microscopy.com} 15, 28 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 15, 28 -- To: {Microscopy-at-MSA.Microscopy.Com} 15, 28 -- X-OriginalArrivalTime: 21 May 2008 19:13:10.0350 (UTC) FILETIME=[B55F0EE0:01C8BB76] 15, 28 -- Content-Transfer-Encoding: 8bit 15, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4LJDB8h011095 ==============================End of - Headers==============================
There are numerous strategies that might be worth a try for your samples. It will require significant trial and error to determine what works best for your samples and analytical needs. All will have advantages and disadvantages and prep artifacts so you will need to experiment and decide what produces the best dispersion (particle loading, particle separation, substrate) for your samples. In general, if you are starting with a dry powder there are a few paths you might try (in no particular order).
1. Dry "puffing" of the sample in a box with your sample substrate. A home-made "puffer" box is pretty cheap and easy to make. You basically use compressed air to puff the dry sample into the air around a sample substrate and allow it to settle. Works well for narrow size distributions of larger particles (~500nm or greater).
2. Resuspension and filtration. Suspend your sample in water (or other solvent) with a surfactant, ultrasonic briefly, and filter onto polycarbonate membrane filter. Remove a section of the filter, place it on a substrate and carbon coat. This is a good general purpose method, but dispersion of particles can be difficult with some minerals (clays in particular). Your sample will also end up on a carbon-rich substrate, but methods are available to produce carbon films supporting particulate on TEM grids (See any of the TEM asbestos analysis methods)
3. Spray mounting. This is basically making a heavy suspension of your sample in a solvent and airbrushing it onto a suitable substrate. One of the microscopy supply vendors makes a spray mounting device. Makes good dispersions of fine particulate, but introduces the possibility of size fractionation during prep.
4. Drop mounting. Sounds like you have already tried this, but if you try different solvents and try heating your substrate slightly to allow for rapid evaporation it might work.
5. Film casting. Suspend sample in collodion solution (~2% or less) and cast a thin film on H20 surface. Pick the film off the water with a polished substrate and heat to sublimate the collodion. This takes a lot of trial and error to get the technique down and to figure out the best particle loading. Your particles also need to be able to withstand higher temperatures and need to be insoluble in H2O.
Those are the few that come to mind. There is opportunity for significant amounts of creativity so have fun playing with this. Just beware that some will introduce significant prep artifacts depending on the particle size.
Lehigh used to offer a course called "Particle Characterization and Preparation, Microscopy and Analysis" during their summer Microscopy School. It was taught by John Small and Cynthia Zeissler et al. from NIST.
All of these general methods can be refined to suit what you have and what you want to do, but all will likely introduce artifacts on the particle size distribution.
Good Luck, Bryan Bandli
-- ~~~~~~~~~~~~~~~~~~~~ Bryan Bandli SEM Laboratory Manager University of Minnesota Duluth Life Science 93
Mail: UMD Geological Sciences 229 Heller Hall 1114 Kirby Dr. Duluth, MN 55812-3036
Phone: 218-726-7362 Fax: 218-726-8275
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I am looking to rent, borrow or purchase a faraday cup and specimen rod to fit our JEOL 1200 EX microscope. I have already tried JEOL USA without success.
I would welcome advice on how to make this contraption myself if it becomes necessary.
Thanks
Marcia Reid
Health Sciences EM Facility, HSc 3N38 McMaster University 1200 Main St. W., Hamilton L8N 3Z5
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While I am not familiar with the 1200, if the TEM can use a SEM cup with their own flavor of blank mounts, try an un-mounted SEM cup. An example is
http://www.tedpella.com/Calibrat_html/faraday.htm
and at a minimum, try the #651 ($118US). Zeiss also makes a pin stub Faraday Cup. If you don't have specimen current reading you can try the probe current monitor made by K.E. Developments, UK
http://www.kedev.co.uk/semaccesories.htm
Hope this helps.
gary g.
At 12:49 PM 5/21/2008, you wrote:
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It might be worthwhile being more persistent with JEOL. Some years ago the excellent people at JEOL Australia managed to persuade the factory to dust off the engineering drawings and make a PCD for me to fit my well-and-truly-superceded 840. The cost was reasonable.They couldn't supply the controller or the picoammeter, but those were easy enough to improvise.
cheers
rtch
} } I am looking to rent, borrow or purchase a faraday cup and specimen rod to } fit our JEOL 1200 EX microscope. I have already tried JEOL USA without } success. } } I would welcome advice on how to make this contraption myself if it becomes } necessary. } } Thanks }
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
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The same rod that fits the 1200EX also fits the following microscopes: 100CX, 200CX, 2000FX. Gatan made (or makes) an analytical stage that has a specimen current monitor in it. It basically is an insulated tip with a feedthru. I had a double-tilt version. In normal operation, there is a miniature BNC grounding plug. When you want to monitor the current, you replace that plug with a cable from a Keithley electrometer, put the beam in the hole and measure the current. If you know of anyone that has that type of stage on any one of these microscopes, there's your answer. You would also need to borrow, rent, or steal the electrometer.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: westm-at-mcmaster.ca [mailto:westm-at-mcmaster.ca] Sent: Wednesday, May 21, 2008 12:51 PM To: Walck-at-SouthBayTech.com
I am looking to rent, borrow or purchase a faraday cup and specimen rod to fit our JEOL 1200 EX microscope. I have already tried JEOL USA without success.
I would welcome advice on how to make this contraption myself if it becomes necessary.
Thanks
Marcia Reid
Health Sciences EM Facility, HSc 3N38 McMaster University 1200 Main St. W., Hamilton L8N 3Z5
==============================Original Headers============================== 6, 31 -- From westm-at-mcmaster.ca Wed May 21 14:48:15 2008 6, 31 -- Received: from coriana6.cis.mcmaster.ca (coriana6.CIS.McMaster.CA [130.113.128.17]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4LJmFfH005768 6, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2008 14:48:15 -0500 6, 31 -- Received: from Gorash7.UTS.McMaster.CA (Gorash7.UTS.mcmaster.ca [130.113.196.61]) 6, 31 -- by coriana6.cis.mcmaster.ca (8.13.7/8.13.7) with ESMTP id m4LJm9KK013575 6, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2008 15:48:14 -0400 (EDT) 6, 31 -- Received: from cgpsrv2.cis.mcmaster.ca (univmail.CIS.McMaster.CA [130.113.64.46]) 6, 31 -- by Gorash7.UTS.McMaster.CA (8.13.7/8.13.7) with ESMTP id m4LJllDr008571 6, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 21 May 2008 15:47:51 -0400 6, 31 -- Received: from [130.113.154.38] (HELO pc1arsenaultl) 6, 31 -- by cgpsrv2.cis.mcmaster.ca (CommuniGate Pro SMTP 4.3.12) 6, 31 -- with SMTPS id 212334246 for Microscopy-at-microscopy.com; Wed, 21 May 2008 15:47:49 -0400 6, 31 -- Message-ID: {001101c8bb7b$908cfeb0$269a7182-at-pc1arsenaultl} 6, 31 -- From: "Marcia Reid" {westm-at-mcmaster.ca} 6, 31 -- To: {Microscopy-at-microscopy.com} 6, 31 -- Subject: In search of a faraday cup and rod to fit JEOL 1200 6, 31 -- Date: Wed, 21 May 2008 15:47:55 -0400 6, 31 -- MIME-Version: 1.0 6, 31 -- Content-Type: text/plain; 6, 31 -- format=flowed; 6, 31 -- charset="iso-8859-1"; 6, 31 -- reply-type=original 6, 31 -- Content-Transfer-Encoding: 7bit 6, 31 -- X-Priority: 3 6, 31 -- X-MSMail-Priority: Normal 6, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 6, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 6, 31 -- X-PMX-Version-Mac: 5.4.2.338381, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.21.123155 6, 31 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_300_399 0, BODY_SIZE_5000_LESS 0, USER_AGENT_OE 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_MSMAIL_PRI 0, __HAS_X_MAILER 0, __HAS_X_PRIORITY 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __USER_AGENT_MS_GENERIC 0' 6, 31 -- X-Spam-Flag: NO ==============================End of - Headers==============================
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This Question was submitted to Ask-A-Microscopist by (debra.linton-at-cmich.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, May 21, 2008 at 14:58:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both debra.linton-at-cmich.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Hi - sorry for the mundane questions, but my colleagues and I are disagreeing on a few issues in how we teach our freshman how to use the microsope...
1. When first focusing on scanning power (compound light microscope) should you start with the stage all they way down and move it towards you, or start with it all the way up and move it away?
2. When storing the microscope, should you store it with the stage all the way up, all the way down, or doesn't matter?
Dear All, Diaspro Lab proudly announces two Advanced Microscopy events that will be held at Department of Physics, Via Dodecaneso 33, Genova.
1) Workshop on "Aspetti pratici di Microscopia Multifotonica e Nanoscopia", Genova 3 Giugno 2008. Information at http://www.sism.it/scuole_eventi.php, please find the brochure also at www.lambs.it.
2) Principles of Fluorescence Techniques 2008, June 30-July 3, Genova, Italy Please, read more information and access to agenda and slides of the past Courses at http://www.fluorescence-foundation.org/fluorescence/index.htm
All the best AD
---------------------------------------------------- "Water slowly flowed under the sky" (Cesare Pavese) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
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Actually most professional microscopes don't get stored these days as they are far too heavy to move (they even sit on large & heavy granite and steel vibration isolation tables). They just get covered with their supplied dust covers [no wooden boxes either, unless you are using a small lab microscope one in the field]. When teaching I even picked up the school microscope incorrectly at first attempt (you should grab it by it's arched metal backbone) as I hadn't actually picked up a tiddly lab microscope since my school days. You'd have to be pretty butch to pick up an inverted Zeiss Axiovert 200M this way.
Normally with microscopes [after use] you always leave the objectives moved up well away from the sample/slide (with a standard upright microscope design, i.e. objectives above the stage). Ideally you should always return to this position when rotating objectives, unless you know the sample is in focus (and all the objectives are parfocal*). This prevents the objective striking the stage or slide when rotating between them. Once you have revolved the nose-piece (objective wheel) to the required objective, you bring the objective down into focus. I assume by 'scanning power' you mean the required objective for a given magnification.
Modern motorised scientific microscopes actually raise & drop the objectives automatically as it spins to the new objective. With a cheap lab or school microscope, you simply leave the objective raised up out of harms way for the next user. Thus it would be stored that way. That said all our microscopes here have very scratched objective tips where users have failed to heed this simple advice, Most of our compound microscopes here are 'inverted' with the objectives located underneath the stage for live cell work with Petri dishes, so with these you should drop the objectives down away from the stage as you leave. Fortunately the objective lens is recessed behind a metal lip and generally survives any impacts. The microscope itself won't care where the stage/objective is (unless you wrack hard against the focussing limits), it's just for the protection of the sample [slide] and the objective lenses during first use by the next user.
When you put your sample (slide) on to the stage, bring the objective slowly closer until the specimen is in focus. Experience and familiarity with the microscope will mean that you will soon remember the approximate focussing distance for each objective.
Regards
Keith
*Objectives which are mounted so that only a small adjustment of the bodytube and stage is necessary to focus after changing from one objective to any of the others [i.e. they shouldn't hit the glass slide if you rotate between objectives when one is in focus]. They are mounted in such a way that the back focal plane is in the same position on the optical axis of the microscope for each objective. Objectives used on a rotating nosepiece are usually parfocal. Eyepieces are also parfocal within any given manufacturer.
http://www.charfac.umn.edu/glossary/p.html
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: debra.linton-at-cmich.edu [mailto:debra.linton-at-cmich.edu] Sent: 22 May 2008 01:11 To: kjmorris-at-well.ox.ac.uk
This Question was submitted to Ask-A-Microscopist by (debra.linton-at-cmich.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, May 21, 2008 at 14:58:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both debra.linton-at-cmich.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Hi - sorry for the mundane questions, but my colleagues and I are disagreeing on a few issues in how we teach our freshman how to use the microsope...
1. When first focusing on scanning power (compound light microscope) should you start with the stage all they way down and move it towards you, or start with it all the way up and move it away?
2. When storing the microscope, should you store it with the stage all the way up, all the way down, or doesn't matter?
Debra, I think Keith has a good approach in terms of storage, but I was taught (many years ago) that when initially focusing a compound microscope, visually bring the stage up (or the tube down)until they are very close. Then, while looking through the ocular, only move the stage down or the tube up so you don't crash the objective into the slide. This protects both the specimen and the microscope.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: debra.linton-at-cmich.edu [mailto:debra.linton-at-cmich.edu] Sent: Wednesday, May 21, 2008 8:06 PM To: kenconverse-at-qualityimages.biz
This Question was submitted to Ask-A-Microscopist by (debra.linton-at-cmich.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, May 21, 2008 at 14:58:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both debra.linton-at-cmich.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Hi - sorry for the mundane questions, but my colleagues and I are disagreeing on a few issues in how we teach our freshman how to use the microsope...
1. When first focusing on scanning power (compound light microscope) should you start with the stage all they way down and move it towards you, or start with it all the way up and move it away?
2. When storing the microscope, should you store it with the stage all the way up, all the way down, or doesn't matter?
Yes I was only talking about how you should ideally leave the microscope for the next user [whether stored or not between use]. With the objective wracked up away from the specimen the next user would normally start with a 4x or 10x air objective, and with these the working distance is so long that you won't be anywhere near the specimen anyway. Once focussed, assuming the microscope was purchased during the last 100 years amd all the objectives are parfocal, you can then switch straight to the high mag lens which will now need minimal fine focus adjustment. If you want to go straight to a high mag objective you can, as Ken suggests, look externally at the reflection of the objective in the coverslip and slowly move the 'two' tips together to get very very close to the coverlip. You now look down the eyepieces and use the fine focus to slowly move away from the sample until focus is achieved - although make sure you know which way you are turning the focus knob. In many cases though there will already be immersion oil on the coverslip from previous use that will confuse things. So I always start with a low mag air objective [assuming you have a standard upright microscope and the specimen has sufficient size/contrast] as it will still focus through any immersion oil. Once focussed, you can switch to the high mag oil immersion objective, refocus, and then set up Koehlar illumination [condensor height] if required.
However most of our large research microscopes these days in biology are of inverted configuration [objectives under the stage] where you can't see squat in terms of the objective and coverslip as it's mostly all hidden under the stage/slide. Plus you can't mix say 60x oil immserion and 10x air objectives so freely as gravity will ensure your low mag air objective gets contaminated with oil. There's still plenty of tricks though to help get the objective to the specimen focus point with the minimum of fuss but they are really beyond the brief of the question.
Plus there's always the advice I once saw on a notice-board outside a small church in Liverpool [while a student there in the mid 1970s]: "For best results, always follow the makers instructions".
Regards
Keith
Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Debra, } I think Keith has a good approach in terms of storage, but I was taught } (many years ago) that when initially focusing a compound microscope, } visually bring the stage up (or the tube down)until they are very close. } Then, while looking through the ocular, only move the stage down or the } tube } up so you don't crash the objective into the slide. This protects both } the } specimen and the microscope. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: debra.linton-at-cmich.edu [mailto:debra.linton-at-cmich.edu] } Sent: Wednesday, May 21, 2008 8:06 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] AskAMicroscopist: Using a microscope Questions } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by } (debra.linton-at-cmich.edu) } from } http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html } on Wednesday, May 21, 2008 at 14:58:08 } Remember to consider the Grade/Age of the student when considering the } Question } --------------------------------------------------------------------------- } Please reply to both debra.linton-at-cmich.edu as well as to the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: debra.linton-at-cmich.edu } Name: Debra Linton } } Organization: Mid-Michigan Community College } } Education: Graduate College } } Location: Mt. Pleasant, MI } } Title: Using a microscope } } Question: Hi - sorry for the mundane questions, but my colleagues and } I are disagreeing on a few issues in how we teach our freshman how to } use the microsope... } } 1. When first focusing on scanning power (compound light microscope) } should you start with the stage all they way down and move it towards } you, or start with it all the way up and move it away? } } 2. When storing the microscope, should you store it with the stage } all the way up, all the way down, or doesn't matter? } } Thanks, } Deb Linton } } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Wed May 21 19:03:37 2008 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4M03afb003815 } 11, 11 -- for {microscopy-at-microscopy.com} ; Wed, 21 May 2008 19:03:37 } -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240806c45a68b142df-at-[206.69.208.22]} } 11, 11 -- Date: Wed, 21 May 2008 19:03:34 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: debra.linton-at-cmich.edu (by way of Ask-A-Microscopist) } 11, 11 -- Subject: AskAMicroscopist: Using a microscope Questions } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } } } } } ==============================Original } Headers============================== } 23, 25 -- From kenconverse-at-qualityimages.biz Thu May 22 17:38:42 2008 } 23, 25 -- Received: from mta11.adelphia.net (mta11.adelphia.net } [68.168.78.205]) } 23, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4MMcfXY016556 } 23, 25 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 22 May 2008 17:38:42 } -0500 } 23, 25 -- Received: from Ken ([72.227.100.25]) by mta11.adelphia.net } 23, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) } with ESMTP } 23, 25 -- id {20080522223712.EOTX15333.mta11.adelphia.net-at-Ken} ; } 23, 25 -- Thu, 22 May 2008 18:37:12 -0400 } 23, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 23, 25 -- To: {debra.linton-at-cmich.edu} , "MSA Listserver" } {Microscopy-at-MSA.Microscopy.Com} } 23, 25 -- Subject: RE: [Microscopy] AskAMicroscopist: Using a microscope } Questions } 23, 25 -- Date: Thu, 22 May 2008 18:38:32 -0400 } 23, 25 -- Message-ID: {000001c8bc5c$90ce2020$6401a8c0-at-Ken} } 23, 25 -- MIME-Version: 1.0 } 23, 25 -- Content-Type: text/plain; } 23, 25 -- charset="us-ascii" } 23, 25 -- X-Priority: 3 (Normal) } 23, 25 -- X-MSMail-Priority: Normal } 23, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 } 23, 25 -- Importance: Normal } 23, 25 -- Thread-Index: Aci7n6wBdd3X1ygVTYqlvsGlXqlkgwAvBSWA } 23, 25 -- In-Reply-To: {200805220006.m4M06PSB007971-at-ns.microscopy.com} } 23, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 23, 25 -- Content-Transfer-Encoding: 8bit } 23, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4MMcfXY016556 } ==============================End of - } Headers============================== }
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
Eddy asked for advice regarding AFM systems appropriate "to locate and image nanomaterials, such as nanoparticles and nanowires ...on silicon dioxide chips". I have been using NanoScope brand AFMs (made by Veeco Metrology/Digital Instruments) since they were first introduced to the market in 1990. Our lab has done more than 1000 projects on more than 100 different kinds of materials and devices, ranging from silicon wafers to food containers, from DNA-protein complexes to DVDs. I have been happy with the capability and reliability of the NanoScope equipment. We also buy, refurbish, sell, install and support used NanoScope AFMs. I suggest you contact me offlist for further details.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- X-from: ess26-at-runbox.com To: donc-at-asmicro.com Sent: Tuesday, May 20, 2008 9:29 PM
Hi Scott,
Thanks for the feedback. I can see that there is some kind of consensus from your reply and the others on the advantages of Asylum's AFM for research. I will see if my budget allow me to get a deal from them. Otherwise, Veeco seems also a decent choice with improving their customer support and service (hope they are reading this!).
Thanks a lot to everybody for your replies.
Eddy
----- Start Original Message ----- Sent: Tue, 20 May 2008 09:59:39 -0500 X-from: "Scott Maclaren" {maclaren-at-mrl.uiuc.edu} To: {ess26-at-runbox.com}
Hi! The first advice I would give is to start the focusing with the lowest magnification (usually 5x or 10x), then changing the objectives (magnification) usually keeps your sample more or less focused. As stated start with the stage furthest of the objective and move it slowly toward the objective. At 5x/10x, you have no risk to hit the objective at all. In any case while changing the objective it makes common sense to check if the objective is going to hit the sample (or any part of the scope for an inverted)!! For storing the position of the stage is not important, but take care of the following: - remove the sample from the stage - clean the lenses (event. oil from 100x objective) - protect from dust BTW, don't forget to teach to make a Koehler illumination!! Happy microscoping Stephane
----- Original Message ---- X-from: "debra.linton-at-cmich.edu" {debra.linton-at-cmich.edu} To: nizets2-at-yahoo.com Sent: Thursday, May 22, 2008 2:07:55 AM
This Question was submitted to Ask-A-Microscopist by (debra.linton-at-cmich.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, May 21, 2008 at 14:58:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both debra.linton-at-cmich.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Hi - sorry for the mundane questions, but my colleagues and I are disagreeing on a few issues in how we teach our freshman how to use the microsope...
1. When first focusing on scanning power (compound light microscope) should you start with the stage all they way down and move it towards you, or start with it all the way up and move it away?
2. When storing the microscope, should you store it with the stage all the way up, all the way down, or doesn't matter?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ykwu-at-hkbu.edu.hk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ykwu-at-hkbu.edu.hk Name: Winnie Wu
Organization: Hong Kong Baptist University
Title-Subject: [Filtered] XRD reference materials - line position
Question: Dear all,
Besides my SEM work I am in charge of an XRD lab.
Not really being a matter of microscopy, I can't find the similar newsgroup in XRD field.
I am looking for the XRD reference materials (SRM640c - Si line position or SRM 660a - Line Position LaB6) from NIST Standard Reference Materials.
However, both standards have been out of stock for over six months as stated at NIST official web-site.
I would be appricated if someone would have some suggestions and also information on where one could acquire compatible standards for XRD.
I am posting on behalf of a colleague, Dr. Christine Broadbridge - please respond to: broadbridge-at-southernct.edu {mailto:broadbridge-at-southernct.edu} .
This is a part-time position with flexible hours up to 19 hrs per week at Southern Connecticut State University in New Haven CT at an hourly rate commensurate with experience. Applications will be accepted until the position is filled. Ann H. Lehman Electron Microscopy Facility at Trinity College 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman {http://www.trincoll.edu/~alehman}
CRISP (http://www.crisp.yale.edu/) - the Center for Research on Interface Structures and Phenomena, an NSF funded Materials Research Science and Engineering Center (at Yale and Southern Connecticut State University) - is seeking a part-time NanoCharacterization Facility Manager with expertise in analytical Transmission and Scanning Electron Microscopy. The Facility is primarily housed at Southern CT State University, with access to CRISP facilities at Yale University. Our emphasis is on materials sample preparation and characterization, but life sciences applications are also of interest. This position requires a self-motivated, detail-oriented professional with broad microscopy and sample preparation skills who can provide expertise in a range of core materials characterization capabilities.
Responsibilities:
Responsible for the operation, testing and maintenance of equipment and processes in the NanoCharacterization Facility at SCSU. Strengthen Facility's analytical capabilities. Initiate and promote scientific publication of results. Train faculty, staff, and students in the use of highly technical equipment for materials research applications. Develop new protocols as necessary to serve Facility goals. Act as a consultant for users to process, image and analyze specimens and to interpret data. Instruct and qualify users wishing to process samples on their own. Serve as liaison with CRISP personnel at Yale University as well as other external users.
Specific duties:
Train and supervise users working with Facility equipment.
Optimize and document equipment use, condition, and maintenance.
Mentor and advise users in interpretation of results.
Responsible for scheduling and access to equipment within the EM lab.
Qualifications:
Advanced degree in a physical or engineering science, with minimum of ten years' related experience. Understands the theoretical aspects of EM and has demonstrated outstanding skill and performance in the field. Has demonstrated experience with user training, routine maintenance and repair of equipment, and record-keeping. Has advanced computer literacy in both PC and Macintosh environments, familiarity with Microsoft Office programs and digital imaging software, has the ability to grasp new concepts quickly, is willing to learn new systems, is a team player, is able to interact with a diverse population, and has complete mastery of the use of written and spoken English for technical discussion and user training.
Interested candidates should send a resume, references and statement of interest in PDF format to: broadbridge-at-southernct.edu (Dr. Christine C. Broadbridge, Prof. of Physics; CRISP Education Director, Southern Connecticut State University; 501 Crescent Street, New Haven, CT 06515; Tel. 203-392-6461)
SCSU is an Affirmative Action/Equal Employment Opportunity employer. The University seeks to enhance the diversity of its faculty and staff. People of color, women and persons with disabilities are strongly encouraged to apply.
==============================Original Headers============================== 22, 26 -- From Ann.Lehman-at-trincoll.edu Tue May 27 11:53:57 2008 22, 26 -- Received: from wsmtp1.cc.trincoll.edu (wsmtp2.cc.trincoll.edu [157.252.10.109]) 22, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4RGrvpi011161 22, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2008 11:53:57 -0500 22, 26 -- Received: from hickory.cc.trincoll.edu ([157.252.15.139]) by wsmtp1.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); 22, 26 -- Tue, 27 May 2008 12:54:00 -0400 22, 26 -- Received: from exbe1.cc.trincoll.edu ([157.252.15.111]) by hickory.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); 22, 26 -- Tue, 27 May 2008 12:54:00 -0400 22, 26 -- Content-class: urn:content-classes:message 22, 26 -- MIME-Version: 1.0 22, 26 -- Content-Type: text/plain; 22, 26 -- charset="iso-8859-1" 22, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 26 -- Subject: Part-time Academic/Technical Job Posting 22, 26 -- Date: Tue, 27 May 2008 12:54:00 -0400 22, 26 -- Message-ID: {8CF6A92CB628444FB3C757618CD280390565A81B-at-exbe1.cmpcntr.tc.trincoll.edu} 22, 26 -- X-MS-Has-Attach: 22, 26 -- X-MS-TNEF-Correlator: 22, 26 -- Thread-Topic: Part-time Academic/Technical Job Posting 22, 26 -- Thread-Index: Aci7hv9OzN5MV8pHSe2jtnZOM9JoXgEjrq8q 22, 26 -- References: {200805212109.m4LL9dNr032404-at-ns.microscopy.com} 22, 26 -- From: "Lehman, Ann R" {Ann.Lehman-at-trincoll.edu} 22, 26 -- To: {Microscopy-at-microscopy.com} 22, 26 -- X-OriginalArrivalTime: 27 May 2008 16:54:00.0846 (UTC) FILETIME=[43285AE0:01C8C01A] 22, 26 -- Content-Transfer-Encoding: 8bit 22, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4RGrvpi011161 ==============================End of - Headers==============================
Reminder: Deadline for application is June 1st, 2008.
International Workshop on Electron Crystallography of Membrane Proteins, UC Davis, California, USA, September 7-13, 2008
The workshop speaker list and program can be seen at http://2dx.org/workshop/2008 Registration can be completed at http://2dx.org/workshop/2008/registration
We will lecture electron diffraction processing in IPLT and introduce the new 3D merging functionality of the 2dx software system on this workshop.
Further info: Ben Hankamer (b.hankamer-at-imb.uq.edu.au), Tom Walz (twalz-at-hms.harvard.edu ), Henning Stahlberg (HStahlberg-at-ucdavis.edu), or for local organization Bryant Gipson (BRGipson-at-ucdavis.edu).
Henning Stahlberg, Molecular & Cellular Biology, Briggs Hall 5, University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab), Fax: +1-530-752 3085 mailto:HStahlberg-at-ucdavis.edu http://stahlberglab.ucdavis.edu http://2dx.org _____________________________________________________________
==============================Original Headers============================== 10, 23 -- From HStahlberg-at-ucdavis.edu Tue May 27 13:46:45 2008 10, 23 -- Received: from mx2.ucdavis.edu (mx2.ucdavis.edu [128.120.32.32]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4RIketH028076 10, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2008 13:46:45 -0500 10, 23 -- Received: from [169.237.214.93] ([169.237.214.93]) 10, 23 -- (authenticated bits=0) 10, 23 -- by mx2.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id m4RIk4Tk009021 10, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 10, 23 -- Tue, 27 May 2008 11:46:05 -0700 (PDT) 10, 23 -- Message-Id: {0DF55614-6910-4194-A677-EC825EE1274D-at-ucdavis.edu} 10, 23 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu} 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 10, 23 -- Content-Transfer-Encoding: 7bit 10, 23 -- Mime-Version: 1.0 (Apple Message framework v919.2) 10, 23 -- Subject: Reminder: Deadline approaching for the Workshop on Electron Crystallography of Membrane Proteins 10, 23 -- Date: Tue, 27 May 2008 11:46:04 -0700 10, 23 -- Cc: Ben Hankamer {b.hankamer-at-imb.uq.edu.au} , Tom Walz {TWalz-at-hms.harvard.edu} , 10, 23 -- "Bryant R. Gipson" {BRGipson-at-ucdavis.edu} 10, 23 -- X-Mailer: Apple Mail (2.919.2) 10, 23 -- X-Virus-Scanned: ClamAV version 0.93, clamav-milter version 0.93 on av1 10, 23 -- X-Virus-Status: Clean 10, 23 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.32 ==============================End of - Headers==============================
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Email: laura.lelansky-at-jax.org Name: Laura
Organization: The Jackson Laboratory
Title-Subject: [Filtered] Frontiers in Microscopy: Whole Animal Imaging
Question: June 30-July 3, 2008 The Jackson Laboratory Bar Harbor, Maine USA
Registrations are still being accepted for this four day short course that will cover state-of-the-art approaches to imaging biological systems in vivo. Topic areas will include, but not be limited to MRI, CT, PET, ultrasound, fluorescence/luminescence, and X-ray beamline imaging in the context of phenotyping animal models. A special afternoon session will be devoted to a survey of commercially available technologies. This course is hosted in conjunction with the Institute for Molecular Biophysics and brings together world-leading experts in diverse fields of modern biological microscopy and medical imaging to provide an overview of the cutting edge technologies and to discuss current challenges.
This short course is a terrific value; registration fee is only $450 or $250 for students, post-docs, medical interns
We have arranged for a full-service resort hotel group room rate of only $149.00 per night ñ that is per room, not per person! Bring your family, stay over for the 4th of July festivities and enjoy Acadia National Park! Please Note: The hotel room block rate expires May 31st.
Speakers, registration form, and the course schedule are posted online at http://courses.jax.org/2008/microscopy_08.html
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Email: antoniomiguel.garcia-at-gmail.com Name: Antonio
I am using a confocal microscope BIORAD MRC 1024 for fluorescence imaging. My question is a simple one, the emission filters are named things like DF620/32, OG515 or 585LP. I think DF620/32 is a band pass filter (although I cannot make up what DF stands for), and 585LP a long pass, but what is OG515?
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Email: mlibbee-at-gmail.com Name: Marissa
Title-Subject: [Filtered] "saw-tooth" edges of sem images
Question: Good morning,
I've observed saw-tooth edges (more wavy, less jagged in appearance) of the structures I was imaging on the Hitachi S-4700 last evening. The cross-sections had been exposed to BOE or PAD etch. Is the beam reacting to residual etchant or is this some form of vibration/charging?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Smithcf-at-hotmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, May 27, 2008 at 15:44:03 ---------------------------------------------------------------------------
Email: Smithcf-at-hotmail.com Name: Chuck Smith
Organization: University of Connecticut
Education: 9-12th Grade High School
Location: Storrs, CT USA
Question: I'm teaching a week-long class on biodiversity this summer for middle school/high school students. I'd like one exercise to be a demonstration of biodiversity living on the human body, perhaps by having students collect samples from themselves (skin scrapings, using masking tape to lift samples, samples from carpets, etc.) and looking at these using EM. Our EM department has graciously offered their services to prepare the samples, but I'm in need of information on how and where to collect samples (eyelash mites, dust mites....). Any suggestions would be greatly appreciated.
I think DF is a discriminating filter with very steep-sided passbands, and OG is orange glass that absorbs blue light.
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
On Tue, 27 May 2008, antoniomiguel.garcia-at-gmail.com wrote:
} Organization: UAM } } Title-Subject: [Filtered] Emission filter nomenclature } } Question: Hello! } } I am using a confocal microscope BIORAD MRC 1024 for fluorescence } imaging. My question is a simple one, the emission filters are named } things like DF620/32, OG515 or 585LP. I think DF620/32 is a band pass } filter (although I cannot make up what DF stands for), and 585LP a } long pass, but what is OG515?
==============================Original Headers============================== 4, 22 -- From wpchan-at-u.washington.edu Tue May 27 22:11:22 2008 4, 22 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S3BM7b012284 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 27 May 2008 22:11:22 -0500 4, 22 -- Received: from homer21.u.washington.edu (homer21.u.washington.edu [140.142.12.133]) 4, 22 -- by mxout7.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m4S3BLph007624 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 22 -- Tue, 27 May 2008 20:11:22 -0700 4, 22 -- Received: from localhost (wpchan-at-localhost) 4, 22 -- by homer21.u.washington.edu (8.13.7+UW06.06/8.13.7+Submit) with ESMTP id m4S3BL29027127; 4, 22 -- Tue, 27 May 2008 20:11:21 -0700 4, 22 -- Date: Tue, 27 May 2008 20:11:21 -0700 (PDT) 4, 22 -- From: "W. Chan" {wpchan-at-u.washington.edu} 4, 22 -- To: microscopy-at-microscopy.com, antoniomiguel.garcia-at-gmail.com 4, 22 -- Subject: Re: [Microscopy] viaWWW: Emission filter nomenclature 4, 22 -- In-Reply-To: {200805280231.m4S2Vk2S008659-at-ns.microscopy.com} 4, 22 -- Message-ID: {Pine.LNX.4.64.0805272005020.31925-at-homer21.u.washington.edu} 4, 22 -- References: {200805280231.m4S2Vk2S008659-at-ns.microscopy.com} 4, 22 -- MIME-Version: 1.0 4, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 4, 22 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.27.195753 4, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_700_799 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
A picture is worth a thousand words - any chance you can put one where people can see it and send a link?
What working distance are you using? If I work at high mags and long working distances, the 60 cycle fields will do that to my images. Shorten the working distance if possible and see if it gets better. I also have an old vacuum meter that will generate that effect if used while imaging - any new equipment or anything relocated closer to the column?
Dale
mlibbee-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa } } Title-Subject: [Filtered] "saw-tooth" edges of sem images } } Question: Good morning, } } I've observed saw-tooth edges (more wavy, less jagged in appearance) } of the structures I was imaging on the Hitachi S-4700 last evening. } The cross-sections had been exposed to BOE or PAD etch. Is the beam } reacting to residual etchant or is this some form of } vibration/charging? } } Thanks, } Marissa } } Login Host: 63.163.107.100 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Tue May 27 21:27:18 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S2RHK5027956 } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 May 2008 21:27:18 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240802c4627372d325-at-[206.69.208.22]} } 7, 11 -- Date: Tue, 27 May 2008 21:27:15 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: "saw-tooth" edges of sem images } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 20 -- From dac-at-research.umass.edu Tue May 27 22:38:29 2008 5, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S3cTEP026138 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, 27 May 2008 22:38:29 -0500 5, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 5, 20 -- (authenticated bits=0) 5, 20 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m4S3cRS6010652 5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 5, 20 -- Tue, 27 May 2008 23:38:28 -0400 5, 20 -- Message-ID: {483CE1E9.2080400-at-research.umass.edu} 5, 20 -- Date: Tue, 27 May 2008 23:39:05 -0500 5, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 5, 20 -- MIME-Version: 1.0 5, 20 -- To: mlibbee-at-gmail.com, Microscopy List {microscopy-at-microscopy.com} 5, 20 -- Subject: Re: [Microscopy] viaWWW: "saw-tooth" edges of sem images 5, 20 -- References: {200805280237.m4S2b9t8026574-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200805280237.m4S2b9t8026574-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
How do you dry carbon paints fast? I normally glue my rock samples a day prior to imaging them. However, some wants to image them right away when I receive their specimens. Any recommendation of a good "dryer"??
Thanks.
Melina Miralles PGSc Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 6, 27 -- From mmiralles-at-pi.ac.ae Wed May 28 00:43:19 2008 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S5hH7g013091 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 00:43:18 -0500 6, 27 -- X-IronPort-AV: E=Sophos;i="4.27,552,1204488000"; 6, 27 -- d="scan'208";a="423459" 6, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 6, 27 -- by mx1.pi.ac.ae with ESMTP; 28 May 2008 09:33:05 +0400 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Wed, 28 May 2008 09:43:15 +0400 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="windows-1256" 6, 27 -- Subject: Drying Carbon Paints 6, 27 -- Date: Wed, 28 May 2008 09:43:14 +0400 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B047A6AA4-at-pi-exm.PI.AC.AE} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Drying Carbon Paints 6, 27 -- Thread-Index: AcjAhbjrTkYyFe+GRc6dDRT3X41RAA== 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 28 May 2008 05:43:15.0982 (UTC) FILETIME=[B9C332E0:01C8C085] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4S5hH7g013091 ==============================End of - Headers==============================
Hello, When we are in hurry, we are using an old (over 30 years) vacuum dryer for TEM photo material. It works fine. I think, any glass dessicator connected to membrane vacuum pump or water jet air ejector should work, too.
Best regards Oldrich
------------------------------------------ Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 - Krc
On 28 May 2008 at 0:46, mmiralles-at-pi.ac.ae wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hello, } } How do you dry carbon paints fast? } I normally glue my rock samples a day prior to imaging them. However, some wants to image them right away when I receive their specimens. Any recommendation of a good "dryer"?? } } Thanks. } } Melina Miralles } PGSc Lab Technician } The Petroleum Institute } Abu Dhabi, UAE } } } } ==============================Original Headers============================== } 6, 27 -- From mmiralles-at-pi.ac.ae Wed May 28 00:43:19 2008 } 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S5hH7g013091 } 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 00:43:18 -0500 } 6, 27 -- X-IronPort-AV: E=Sophos;i="4.27,552,1204488000"; } 6, 27 -- d="scan'208";a="423459" } 6, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) } 6, 27 -- by mx1.pi.ac.ae with ESMTP; 28 May 2008 09:33:05 +0400 } 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); } 6, 27 -- Wed, 28 May 2008 09:43:15 +0400 } 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 6, 27 -- Content-class: urn:content-classes:message } 6, 27 -- MIME-Version: 1.0 } 6, 27 -- Content-Type: text/plain; } 6, 27 -- charset="windows-1256" } 6, 27 -- Subject: Drying Carbon Paints } 6, 27 -- Date: Wed, 28 May 2008 09:43:14 +0400 } 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B047A6AA4-at-pi-exm.PI.AC.AE} } 6, 27 -- X-MS-Has-Attach: } 6, 27 -- X-MS-TNEF-Correlator: } 6, 27 -- Thread-Topic: Drying Carbon Paints } 6, 27 -- Thread-Index: AcjAhbjrTkYyFe+GRc6dDRT3X41RAA== } 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} } 6, 27 -- To: {microscopy-at-microscopy.com} } 6, 27 -- X-OriginalArrivalTime: 28 May 2008 05:43:15.0982 (UTC) FILETIME=[B9C332E0:01C8C085] } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4S5hH7g013091 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 25 -- From benada-at-biomed.cas.cz Wed May 28 01:36:45 2008 8, 25 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S6aibl027819 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 01:36:44 -0500 8, 25 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 8, 25 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 43D17928001; 8, 25 -- Wed, 28 May 2008 08:36:33 +0200 (CEST) 8, 25 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 8, 25 -- Organization: Institute of Microbiology 8, 25 -- To: mmiralles-at-pi.ac.ae 8, 25 -- Date: Wed, 28 May 2008 08:36:33 +0200 8, 25 -- MIME-Version: 1.0 8, 25 -- Subject: Re: [Microscopy] Drying Carbon Paints 8, 25 -- CC: microscopy-at-microscopy.com 8, 25 -- Message-ID: {483D1991.20314.1C7074-at-benada.biomed.cas.cz} 8, 25 -- Priority: normal 8, 25 -- In-reply-to: {200805280546.m4S5k1Ig017021-at-ns.microscopy.com} 8, 25 -- References: {200805280546.m4S5k1Ig017021-at-ns.microscopy.com} 8, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 8, 25 -- Content-type: text/plain; charset=ISO-8859-2 8, 25 -- Content-description: Mail message body 8, 25 -- X-Antivirus: avast! (VPS 080527-1, 27.05.2008), Outbound message 8, 25 -- X-Antivirus-Status: Clean 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id m4S6aibl027819 ==============================End of - Headers==============================
It's the lower right model. It's a kind used to warm chicken or other little animals in farming.
It's fixed on a support, with an aluminium foil mantel, and supplied trough a enlarger time switch. Playing with the distance of the sample, the temperature can be varied between 30-60°, and silver dagg needs between 20-50 s to dry. Carbon should dry faster.
Costs the price of the bulbe, a few euro, the other material was recycled.... Cheap and usefull !
But the best drying way would be to try to "educate" the users to avoid the last minute observations. For urgent work one need a delay !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
I'm curious about this 19 hrs per week issue. Why 19 instead of 20 hrs per week? Does it have anything to do with insurance and benefit purposes?
Best, Ayten.
On Tue, May 27, 2008 at 8:02 PM, {Ann.Lehman-at-trincoll.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I am posting on behalf of a colleague, Dr. Christine Broadbridge - please respond to: broadbridge-at-southernct.edu {mailto:broadbridge-at-southernct.edu} . } } This is a part-time position with flexible hours up to 19 hrs per week at Southern Connecticut State University in New Haven CT at an hourly rate commensurate with experience. Applications will be accepted until the position is filled. } Ann H. Lehman } Electron Microscopy Facility at Trinity College } 300 Summit Street } Hartford CT 06106 } v. 860-297-4289 } f. 860-297-2538 } e. ann.lehman-at-trincoll.edu } w. http://www.trincoll.edu/~alehman } {http://www.trincoll.edu/~alehman} } } } } --JOB POSTING-- } } } } Part-time Position: NanoCharacterization Facility Manager } } CRISP (http://www.crisp.yale.edu/) - the Center for Research on Interface Structures and Phenomena, an NSF funded Materials Research Science and Engineering Center (at Yale and Southern Connecticut State University) - is seeking a part-time NanoCharacterization Facility Manager with expertise in analytical Transmission and Scanning Electron Microscopy. The Facility is primarily housed at Southern CT State University, with access to CRISP facilities at Yale University. Our emphasis is on materials sample preparation and characterization, but life sciences applications are also of interest. This position requires a self-motivated, detail-oriented professional with broad microscopy and sample preparation skills who can provide expertise in a range of core materials characterization capabilities. } } } } Responsibilities: } } Responsible for the operation, testing and maintenance of equipment and processes in the NanoCharacterization Facility at SCSU. Strengthen Facility's analytical capabilities. Initiate and promote scientific publication of results. Train faculty, staff, and students in the use of highly technical equipment for materials research applications. Develop new protocols as necessary to serve Facility goals. Act as a consultant for users to process, image and analyze specimens and to interpret data. Instruct and qualify users wishing to process samples on their own. Serve as liaison with CRISP personnel at Yale University as well as other external users. } } } } Specific duties: } } Train and supervise users working with Facility equipment. } } Optimize and document equipment use, condition, and maintenance. } } Mentor and advise users in interpretation of results. } } Responsible for scheduling and access to equipment within the EM lab. } } } } Qualifications: } } Advanced degree in a physical or engineering science, with minimum of ten years' related experience. Understands the theoretical aspects of EM and has demonstrated outstanding skill and performance in the field. Has demonstrated experience with user training, routine maintenance and repair of equipment, and record-keeping. Has advanced computer literacy in both PC and Macintosh environments, familiarity with Microsoft Office programs and digital imaging software, has the ability to grasp new concepts quickly, is willing to learn new systems, is a team player, is able to interact with a diverse population, and has complete mastery of the use of written and spoken English for technical discussion and user training. } } } } Interested candidates should send a resume, references and statement of interest in PDF format to: broadbridge-at-southernct.edu (Dr. Christine C. Broadbridge, Prof. of Physics; CRISP Education Director, Southern Connecticut State University; 501 Crescent Street, New Haven, CT 06515; Tel. 203-392-6461) } } SCSU is an Affirmative Action/Equal Employment Opportunity employer. The University seeks to enhance the diversity of its faculty and staff. People of color, women and persons with disabilities are strongly encouraged to apply. } } } ==============================Original Headers============================== } 22, 26 -- From Ann.Lehman-at-trincoll.edu Tue May 27 11:53:57 2008 } 22, 26 -- Received: from wsmtp1.cc.trincoll.edu (wsmtp2.cc.trincoll.edu [157.252.10.109]) } 22, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4RGrvpi011161 } 22, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 27 May 2008 11:53:57 -0500 } 22, 26 -- Received: from hickory.cc.trincoll.edu ([157.252.15.139]) by wsmtp1.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); } 22, 26 -- Tue, 27 May 2008 12:54:00 -0400 } 22, 26 -- Received: from exbe1.cc.trincoll.edu ([157.252.15.111]) by hickory.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); } 22, 26 -- Tue, 27 May 2008 12:54:00 -0400 } 22, 26 -- Content-class: urn:content-classes:message } 22, 26 -- MIME-Version: 1.0 } 22, 26 -- Content-Type: text/plain; } 22, 26 -- charset="iso-8859-1" } 22, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 22, 26 -- Subject: Part-time Academic/Technical Job Posting } 22, 26 -- Date: Tue, 27 May 2008 12:54:00 -0400 } 22, 26 -- Message-ID: {8CF6A92CB628444FB3C757618CD280390565A81B-at-exbe1.cmpcntr.tc.trincoll.edu} } 22, 26 -- X-MS-Has-Attach: } 22, 26 -- X-MS-TNEF-Correlator: } 22, 26 -- Thread-Topic: Part-time Academic/Technical Job Posting } 22, 26 -- Thread-Index: Aci7hv9OzN5MV8pHSe2jtnZOM9JoXgEjrq8q } 22, 26 -- References: {200805212109.m4LL9dNr032404-at-ns.microscopy.com} } 22, 26 -- From: "Lehman, Ann R" {Ann.Lehman-at-trincoll.edu} } 22, 26 -- To: {Microscopy-at-microscopy.com} } 22, 26 -- X-OriginalArrivalTime: 27 May 2008 16:54:00.0846 (UTC) FILETIME=[43285AE0:01C8C01A] } 22, 26 -- Content-Transfer-Encoding: 8bit } 22, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4RGrvpi011161 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 29 -- From celikaktas-at-gmail.com Wed May 28 02:57:05 2008 6, 29 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.188]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S7v3KE024810 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 02:57:04 -0500 6, 29 -- Received: by fk-out-0910.google.com with SMTP id 18so2687688fks.2 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 00:57:03 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 29 -- bh=EP1oqpoSP4PCoNE9Fy6j3VHZUMiIznKbJefN4vgf5k0=; 6, 29 -- b=gKMt9VKR2Wiqq5wCYZ+BAcajwSMj6NV/sm+PABMh46D/6ZktbtXdIoInid0bWhzxAjmgcMB3UiDvMFfq7KsGEqRn/kj7lMVqTyqUZqLkoYh3TJVLD7ZqyVvZEAaseJPMQf5dTO6/toztH+O3plR1n+TzQNVPiuZNUSLlCQH5mKM= 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 29 -- b=PMc2juYanQAA8WhEoElgVfhOaLZ92+sSbH/3h7IWtsQoDVgNva+zCyC/0136nQQhv0Me9bmZyYcu/SAvsf+uQ96FqMrQcc4Kr9H8Frq87S8JML0adEDp9y0w2mEqaWSyw1p/VzDE0GjP5DAU7rIR0fna5NUN5jqUtzhBguD6mFo= 6, 29 -- Received: by 10.82.177.5 with SMTP id z5mr139836bue.49.1211961423052; 6, 29 -- Wed, 28 May 2008 00:57:03 -0700 (PDT) 6, 29 -- Received: by 10.82.126.2 with HTTP; Wed, 28 May 2008 00:57:02 -0700 (PDT) 6, 29 -- Message-ID: {1075c5c10805280057p58b2c605l5f53e0cc224a2f8-at-mail.gmail.com} 6, 29 -- Date: Wed, 28 May 2008 10:57:02 +0300 6, 29 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} 6, 29 -- To: microscopy {Microscopy-at-microscopy.com} 6, 29 -- Subject: Re: [Microscopy] Part-time Academic/Technical Job Posting 6, 29 -- In-Reply-To: {200805271702.m4RH2Gjb023060-at-ns.microscopy.com} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; charset=UTF-8 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- Content-Disposition: inline 6, 29 -- References: {200805271702.m4RH2Gjb023060-at-ns.microscopy.com} ==============================End of - Headers==============================
We have a problem with an old Zeiss stereomicroscope, a STEMI SR, used for sorting flies. Users have the feeling of being cross-eyed and seeing a shadow image. I've had a look at it for obvious problems and the route cause seems to be related to the adjustment of the distance between the oculars. If you take off the top turret (47-50-89) there are some teeth that ensure the two eyepiece tubes move together. One of these teeth is broken, so they can be adjusted, to some extend, independently. The question is (and this is a long shot) does anybody have any spare parts that they may be willing to part with. I don't think the tooth can be repaired, so it would have to be replaced... but it's now very old, so that will no doubt be a problem. Any suggestions greatly appreciated. Thanks,
Graham Wright Microscopy & Imaging Facility
Temasek Life Sciences Laboratory 1 Research Link National University of Singapore Singapore 117604
2 solutions: - Glue of small sign on your desk, writing: "For urgent work, give me one week. For impossible demands, give me one day". - Rock samples can easily stand medium temperatures, why not bake at 60-80°C (please translate for °F ;-))? I guess it would be ready in no more than 15 min. And it would add the advantage to degas or dry the rock at the same time. Best regards, Stephane
----- Original Message ---- X-from: "mmiralles-at-pi.ac.ae" {mmiralles-at-pi.ac.ae} To: nizets2-at-yahoo.com Sent: Wednesday, May 28, 2008 7:47:49 AM
Hello,
How do you dry carbon paints fast? I normally glue my rock samples a day prior to imaging them. However, some wants to image them right away when I receive their specimens. Any recommendation of a good "dryer"??
Thanks.
Melina Miralles PGSc Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 6, 27 -- From mmiralles-at-pi.ac.ae Wed May 28 00:43:19 2008 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S5hH7g013091 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 00:43:18 -0500 6, 27 -- X-IronPort-AV: E=Sophos;i="4.27,552,1204488000"; 6, 27 -- d="scan'208";a="423459" 6, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 6, 27 -- by mx1.pi.ac.ae with ESMTP; 28 May 2008 09:33:05 +0400 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Wed, 28 May 2008 09:43:15 +0400 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="windows-1256" 6, 27 -- Subject: Drying Carbon Paints 6, 27 -- Date: Wed, 28 May 2008 09:43:14 +0400 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B047A6AA4-at-pi-exm.PI.AC.AE} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Drying Carbon Paints 6, 27 -- Thread-Index: AcjAhbjrTkYyFe+GRc6dDRT3X41RAA== 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 28 May 2008 05:43:15.0982 (UTC) FILETIME=[B9C332E0:01C8C085] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4S5hH7g013091 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 22 -- From nizets2-at-yahoo.com Wed May 28 03:30:10 2008 17, 22 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4S8UAqw019182 17, 22 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 03:30:10 -0500 17, 22 -- Received: (qmail 87602 invoked by uid 60001); 28 May 2008 08:30:09 -0000 17, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 17, 22 -- s=s1024; d=yahoo.com; 17, 22 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 17, 22 -- b=NRyMmv0WGPaZH4FDz7p0MwXnylEcmdyVzKtLjlnogFReBl0wGKIQbPzwyX0FOYKagYvPO+HUnecQFp68YrDej13LjHq4EBNIvN+iqfhjdfavzt1vwDPAIfvIpVlPjuNx/Yb0r9l1U5dLht0AaSkCP8sEmLJvQPXEFI8pJ6e/7Ng=; 17, 22 -- X-YMail-OSG: T4DtRqIVM1kGged6xGLZ65mtN7P2_vcq_CacesbzW1JdvMBF83WLpFzyxP0ljcGKkVemA0c5uefFqamAwMEgG_OiEUpBWDIkO2vxENBBXKXMZQJZY7y3J9q.1ZFaQdGRYjxfZXXGY34OUqeM2Ogmh.vkpy_QsTHOtR8UedRtoyAWLtxs1PwD 17, 22 -- Received: from [80.122.101.100] by web37405.mail.mud.yahoo.com via HTTP; Wed, 28 May 2008 01:30:08 PDT 17, 22 -- X-Mailer: YahooMailRC/975.41 YahooMailWebService/0.7.185 17, 22 -- Date: Wed, 28 May 2008 01:30:08 -0700 (PDT) 17, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 17, 22 -- Subject: Re: [Microscopy] Drying Carbon Paints 17, 22 -- To: mmiralles-at-pi.ac.ae 17, 22 -- Cc: microscopy-at-microscopy.com 17, 22 -- MIME-Version: 1.0 17, 22 -- Content-Type: text/plain; charset=iso-8859-1 17, 22 -- Message-ID: {942862.85443.qm-at-web37405.mail.mud.yahoo.com} 17, 22 -- Content-Transfer-Encoding: 8bit 17, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4S8UAqw019182 ==============================End of - Headers==============================
As already suggested, wet filter paper (Whatman Qualitative grade "1") is ideal. If you hold the piece of wet filter paper at an angle so that it contacts the edge of the underside of the grid that also helps to pull the suspension down rather than straight to the edge. Good luck.
Ted
The EMscope Company Ltd. Mukdahan, Thailand
66-81-739-4259
==============================Original Headers============================== 7, 19 -- From drteddunne-at-yahoo.com Wed May 28 04:21:38 2008 7, 19 -- Received: from web33404.mail.mud.yahoo.com (web33404.mail.mud.yahoo.com [68.142.206.136]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4S9LcZl002115 7, 19 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 04:21:38 -0500 7, 19 -- Received: (qmail 55659 invoked by uid 60001); 28 May 2008 09:21:38 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 7, 19 -- b=n0iQgVeMuvK+t5P6nhZ1r9i/l4KNNfcDTXeeOKdPqNQlCYJOH5pmkR4ltsEcdeLWEV1fv2j7BwW2mHJ0eOWL/r0hGXhU6iVh+LHg1eeo4vabakb9LXVfrBAKamvGcArt2AxhqMtNqy/fsup0u07Igq8r8qwfDCpms7KcRLbO55c=; 7, 19 -- Received: from [125.25.154.171] by web33404.mail.mud.yahoo.com via HTTP; Wed, 28 May 2008 02:21:38 PDT 7, 19 -- X-Mailer: YahooMailRC/975.41 YahooMailWebService/0.7.199 7, 19 -- Date: Wed, 28 May 2008 02:21:38 -0700 (PDT) 7, 19 -- From: ted dunn {drteddunne-at-yahoo.com} 7, 19 -- Subject: Re: [Microscopy] TEM: wicking or blotting grids 7, 19 -- To: LettJ-at-ent.wustl.edu 7, 19 -- Cc: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {191857.55175.qm-at-web33404.mail.mud.yahoo.com} ==============================End of - Headers==============================
Are these artifacts present at all magnifications or just high-mag? I get saw-tooth images at high-mag if my samples are loose on the holder. Samples clamped and fixed to the base don't have this problem.
I believe microscopists call this effect the "jiggies".
Stu Smalinskas, P.E. Metallurgist SKF Plymouth, Michigan --- mlibbee-at-gmail.com wrote: } } Name: Marissa } } Title-Subject: [Filtered] "saw-tooth" edges of sem } images } } Question: Good morning, } } I've observed saw-tooth edges (more wavy, less } jagged in appearance) } of the structures I was imaging on the Hitachi } S-4700 last evening. } The cross-sections had been exposed to BOE or PAD } etch. Is the beam } reacting to residual etchant or is this some form of } } vibration/charging? } } Thanks, } Marissa } }
==============================Original Headers============================== 5, 20 -- From smalinskas-at-yahoo.com Wed May 28 07:46:49 2008 5, 20 -- Received: from web34406.mail.mud.yahoo.com (web34406.mail.mud.yahoo.com [66.163.178.155]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m4SCklVW024951 5, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 28 May 2008 07:46:48 -0500 5, 20 -- Received: (qmail 91538 invoked by uid 60001); 28 May 2008 12:46:45 -0000 5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 20 -- s=s1024; d=yahoo.com; 5, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 20 -- b=JmgOzJETfSrVrwmorO2Bone2jj8z5YwkTxOKtXcH3xx2XgI7U3KuUBCtQX0+qxw5TtXMt3cJsOlk/RqxRBShFSuofz+8fZ+1nWrGxvDE073xlB/Rw7wPL8cX6YQZ+YmAHUBh/lLYVFYTKXt6+nSHp2ZZ1uwm0QEgqNivuqZJZiQ=; 5, 20 -- X-YMail-OSG: IAzCWRUVM1nsHV3APSaL5TsFpZk41tM64YKtToBZHLrM8O6dARxsMcod23S0pHNAUylv0wVNG3ocgqMiQbWPVpbwvyU3Ci18qJad 5, 20 -- Received: from [141.151.33.213] by web34406.mail.mud.yahoo.com via HTTP; Wed, 28 May 2008 05:46:45 PDT 5, 20 -- Date: Wed, 28 May 2008 05:46:45 -0700 (PDT) 5, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 5, 20 -- Subject: Re: [Microscopy] viaWWW: "saw-tooth" edges of sem images 5, 20 -- To: mlibbee-at-gmail.com, microscopy-at-ns.microscopy.com 5, 20 -- In-Reply-To: {200805280228.m4S2SfxW032504-at-ns.microscopy.com} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=iso-8859-1 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- Message-ID: {283557.91519.qm-at-web34406.mail.mud.yahoo.com} ==============================End of - Headers==============================
Is anyone aware of a method of processing/staining tissue so that mitochondria will jump out at you in the resulting TEM images?
One of our clients wants to use Metamorph to count and measure areas of mitochondria in muscle tissue and the program so far has been unable to separate the organelles from the surroundings. They don't want to have to resort to manual tracing of the structures, if they can avoid it. So far, I haven't been able to locate any specific stains for TEM of mitochondria, but maybe there is something similar to silver staining for nerve tissue?
I have run across one reference to using acetonitrile for dehydration, coupled with imidazole-buffered osmium for secondary fixation as a way of emphasizing mitochondria, so I may try that. I would be interested in any experiences someone might have had with that technique.
I may also try conventional, as opposed to microwave, processing, as a way of extracting more tissue components around the mitochondria as a possible way of isolating them visually.
Any suggestions would be greatly appreciated.
And, hey, Happy Belated Norwegian Independence Day (May 17)!
Randy Tindall President, Officers, and Member Half-Norwegian (on my mother's side) Microscopy Society of America W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Wed May 28 08:46:20 2008 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SDkKkU008695 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 08:46:20 -0500 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 9, 23 -- Wed, 28 May 2008 08:46:17 -0500 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: TEM: mitochondria and image analysis 9, 23 -- Date: Wed, 28 May 2008 08:46:17 -0500 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD76A0-at-UM-XMAIL08.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: TEM: mitochondria and image analysis 9, 23 -- Thread-Index: AcjAyTQZYxGo7D75Thaav+zzhHUymw== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 28 May 2008 13:46:17.0924 (UTC) FILETIME=[34589840:01C8C0C9] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4SDkKkU008695 ==============================End of - Headers==============================
Just a thought that is really off the wall - but what about doing it with immunohistochemistry. I've done the immunoEM with DAB in monolayers infected with chlamydia with pre-embedding and it worked well. The basic concept is the same, just a different Ab for a natural occuring enzyme. It may work on LR White embedded muscle tissue. But then again, it may not.
My biochemistry days are far behind, so I am not sure what particular enzyme you would be best to stain for, but I do remember that there are some very specific enzymes for mitochondria, and believe there were some light microscopy tests.
On the other hand, as far as hand tracing, what does the client have students for? ;-)
Paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-392
==============================Original Headers============================== 7, 21 -- From paul_hazelton-at-umanitoba.ca Wed May 28 09:09:16 2008 7, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SE9E7t022684 7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 09:09:15 -0500 7, 21 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 7, 21 -- (authenticated bits=0) 7, 21 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m4SE9BUs012758; 7, 21 -- Wed, 28 May 2008 09:09:12 -0500 (CDT) 7, 21 -- Message-ID: {483D6788.5070805-at-umanitoba.ca} 7, 21 -- Date: Wed, 28 May 2008 09:09:12 -0500 7, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 21 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 7, 21 -- MIME-Version: 1.0 7, 21 -- To: TindallR-at-missouri.edu 7, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] TEM: mitochondria and image analysis 7, 21 -- References: {200805281348.m4SDmbwb011705-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {200805281348.m4SDmbwb011705-at-ns.microscopy.com} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
An acetone dehydration in conventional processing clears a lot of cytoplasm from cells vs. ethanol. Mitochondria, cytoskeletin, ER, golgi etc. all remain but the background is much lighter making the organelles stand out more. I have not tried the other suggestions that you list.
A word of caution that you may already know:
Depending on where the sections are taken from the muscle there can be several hundred% differences in mitochondrial volume/size. I am currently studying muscle cross sections (very close to cross) rather than longitudinal views as originally requested by the investigator because of this.
Interested in seeing others reply. Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} =====
} From: {TindallR-at-missouri.edu} } Reply-To: {TindallR-at-missouri.edu} } Date: Wed, 28 May 2008 08:52:20 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] TEM: mitochondria and image analysis
} Dear Collective, } } Is anyone aware of a method of processing/staining tissue so that } mitochondria will jump out at you in the resulting TEM images? } } One of our clients wants to use Metamorph to count and measure areas of } mitochondria in muscle tissue and the program so far has been unable to } separate the organelles from the surroundings. They don't want to have } to resort to manual tracing of the structures, if they can avoid it. So } far, I haven't been able to locate any specific stains for TEM of } mitochondria, but maybe there is something similar to silver staining } for nerve tissue? } } I have run across one reference to using acetonitrile for dehydration, } coupled with imidazole-buffered osmium for secondary fixation as a way } of emphasizing mitochondria, so I may try that. I would be interested } in any experiences someone might have had with that technique. } } I may also try conventional, as opposed to microwave, processing, as a } way of extracting more tissue components around the mitochondria as a } possible way of isolating them visually. } } Any suggestions would be greatly appreciated. } } And, hey, Happy Belated Norwegian Independence Day (May 17)! } } Randy Tindall } President, Officers, and Member } Half-Norwegian (on my mother's side) Microscopy Society of America } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu
==============================Original Headers============================== 10, 27 -- From connellyps-at-nhlbi.nih.gov Wed May 28 09:11:46 2008 10, 27 -- Received: from nihxway5out.hub.nih.gov (nihxway5out.hub.nih.gov [128.231.90.113]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SEBh6k027668 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 09:11:46 -0500 10, 27 -- X-IronPortListener: Outbound_SMTP 10, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) 10, 27 -- by nihxway5out.hub.nih.gov with ESMTP; 28 May 2008 10:11:43 -0400 10, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 10, 27 -- Wed, 28 May 2008 10:11:35 -0400 10, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 10, 27 -- Wed, 28 May 2008 14:11:34 +0000 10, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 27 -- Date: Wed, 28 May 2008 10:07:57 -0400 10, 27 -- Subject: Re: [Microscopy] TEM: mitochondria and image analysis 10, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 27 -- To: Randy Tindall {TindallR-at-missouri.edu} , 10, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 27 -- Message-ID: {C462DF7D.1D38%connellyps-at-nhlbi.nih.gov} 10, 27 -- Thread-Topic: [Microscopy] TEM: mitochondria and image analysis 10, 27 -- Thread-Index: AcjAzDqneWd05Cy/Ed2hKgANk2Yv1A== 10, 27 -- In-Reply-To: {200805281352.m4SDqK7g016601-at-ns.microscopy.com} 10, 27 -- Mime-version: 1.0 10, 27 -- Content-type: text/plain; 10, 27 -- charset="ISO-8859-1" 10, 27 -- X-OriginalArrivalTime: 28 May 2008 14:11:35.0477 (UTC) FILETIME=[BCE0CE50:01C8C0CC] 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4SEBh6k027668 ==============================End of - Headers==============================
Hi everybody, I have got an old (about 80's, I think) polarizing microscope Zeiss Axioskop (the model with analyzer inserted in a window at 45°). I have to center the four objectives...no problem with the 2.5x, 10x, 20x...but the 40x does not have the two screws to regulate it...how could I center it? Pietro -- ********************************************* Pietro Arico' Dipartimento di Chimica e fisica della Terra ed Applicazioni alle Georisorse ed ai Rischi Naturali (CFTA) University of Palermo VIa Archirafi, 36 Palermo, 90123 - Italy email: arico-at-unipa.it http://www.geopietro.it Tel. +390916161574 (ext. 139) Fax: +390916168376 *********************************************
==============================Original Headers============================== 2, 18 -- From p.arico-at-email.it Wed May 28 10:21:09 2008 2, 18 -- Received: from mail.unipa.it (mail.unipa.it [147.163.1.45]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SFL8RY025808 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 10:21:08 -0500 2, 18 -- Received: from PowerBookG4-17-di-Pietro-Arico.local ([147.163.124.32]) 2, 18 -- by mail.unipa.it (8.13.1/8.13.1) with ESMTP id m4SFL2ox019466 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 17:21:07 +0200 2, 18 -- Message-ID: {483D785C.2010207-at-email.it} 2, 18 -- Date: Wed, 28 May 2008 17:21:00 +0200 2, 18 -- From: =?ISO-8859-15?Q?Pietro_Aric=F2?= {p.arico-at-email.it} 2, 18 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 2, 18 -- MIME-Version: 1.0 2, 18 -- To: Microscopy-at-microscopy.com 2, 18 -- Subject: Zeiss Axioskop - How to center objectives 2, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 2, 18 -- Content-Transfer-Encoding: 8bit 2, 18 -- X-Virus-Scanned: ClamAV 0.93/7213/Thu May 22 10:29:01 2008 on mail.unipa.it 2, 18 -- X-Virus-Status: Clean ==============================End of - Headers==============================
The frequency of the oscillations will be important to diagnosing the problem. If the problem is due to a vibration or a field, the frequency should be fairly constant and uniform from top to bottom in the image. Different scan rates should produce the same frequency. The effect should also be fairly constant for the same mag across different samples.
If the problem is due to charging, the effect may not be uniform throughout the image. It need not have a constant frequency although it might be fairly constant if the microstructure and distribution is fairly uniform. I would also expect the effect to vary with magnification whereas a fixed-frequency interference would not.
Warren Straszheim Iowa State University
-----Original Message----- X-from: mlibbee-at-gmail.com [mailto:mlibbee-at-gmail.com] Sent: Tuesday, May 27, 2008 9:28 PM To: wesaia-at-iastate.edu
Hand held hair dryers work great! In fact, I can't think of any other useful purpose for a hand held hair dryer.......
John Mardinly, Numonyx
-----Original Message----- X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae] Sent: Tuesday, May 27, 2008 10:51 PM To: MARDINLY, A
Hello,
How do you dry carbon paints fast? I normally glue my rock samples a day prior to imaging them. However, some wants to image them right away when I receive their specimens. Any recommendation of a good "dryer"??
Thanks.
Melina Miralles PGSc Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 6, 27 -- From mmiralles-at-pi.ac.ae Wed May 28 00:43:19 2008 6, 27 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4S5hH7g013091 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 00:43:18 -0500 6, 27 -- X-IronPort-AV: E=Sophos;i="4.27,552,1204488000"; 6, 27 -- d="scan'208";a="423459" 6, 27 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 6, 27 -- by mx1.pi.ac.ae with ESMTP; 28 May 2008 09:33:05 +0400 6, 27 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Wed, 28 May 2008 09:43:15 +0400 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="windows-1256" 6, 27 -- Subject: Drying Carbon Paints 6, 27 -- Date: Wed, 28 May 2008 09:43:14 +0400 6, 27 -- Message-ID: {D5603421C6303A46883F87E7968F285B047A6AA4-at-pi-exm.PI.AC.AE} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Drying Carbon Paints 6, 27 -- Thread-Index: AcjAhbjrTkYyFe+GRc6dDRT3X41RAA== 6, 27 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 27 -- To: {microscopy-at-microscopy.com} 6, 27 -- X-OriginalArrivalTime: 28 May 2008 05:43:15.0982 (UTC) FILETIME=[B9C332E0:01C8C085] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4S5hH7g013091 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 29 -- From A.MARDINLY-at-numonyx.com Wed May 28 11:16:24 2008 15, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SGGOVK022826 15, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 28 May 2008 11:16:24 -0500 15, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 15, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 6E8084141B1; 15, 29 -- Wed, 28 May 2008 12:05:38 -0400 (EDT) 15, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 15, 29 -- Wed, 28 May 2008 12:16:23 -0400 15, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 29 -- Content-class: urn:content-classes:message 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; 15, 29 -- charset="iso-8859-1" 15, 29 -- Subject: RE: [Microscopy] Drying Carbon Paints 15, 29 -- Date: Wed, 28 May 2008 12:16:21 -0400 15, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF943F16D-at-EXDRESBENMX012.numonyx.local} 15, 29 -- In-Reply-To: {200805280551.m4S5pHKR025651-at-ns.microscopy.com} 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- Thread-Topic: [Microscopy] Drying Carbon Paints 15, 29 -- Thread-Index: AcjAhuwKvZVmmkE4TkCpd8U8Rzeb6QAVwGqg 15, 29 -- References: {200805280551.m4S5pHKR025651-at-ns.microscopy.com} 15, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 15, 29 -- To: {mmiralles-at-pi.ac.ae} 15, 29 -- Cc: {Microscopy-at-MSA.Microscopy.Com} 15, 29 -- X-OriginalArrivalTime: 28 May 2008 16:16:23.0168 (UTC) FILETIME=[2BE3C800:01C8C0DE] 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4SGGOVK022826 ==============================End of - Headers==============================
Use the 40x as the reference, and center the other three relative to it.
Darrell
p.arico-at-email.it wrote on 05/28/2008 10:22:13 AM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi everybody, } I have got an old (about 80's, I think) polarizing microscope Zeiss } Axioskop (the model with analyzer inserted in a window at 45°). I have } to center the four objectives...no problem with the 2.5x, 10x, 20x...but
} the 40x does not have the two screws to regulate it...how could I center it? } Pietro } -- } ********************************************* } Pietro Arico' } Dipartimento di Chimica e fisica della Terra } ed Applicazioni alle Georisorse ed ai Rischi } Naturali (CFTA) } University of Palermo } VIa Archirafi, 36 } Palermo, 90123 - Italy } email: arico-at-unipa.it } http://www.geopietro.it } Tel. +390916161574 (ext. 139) } Fax: +390916168376 } ********************************************* } } } ==============================Original Headers============================== } 2, 18 -- From p.arico-at-email.it Wed May 28 10:21:09 2008 } 2, 18 -- Received: from mail.unipa.it (mail.unipa.it [147.163.1.45]) } 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m4SFL8RY025808 } 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 10:21:08 -0500 } 2, 18 -- Received: from PowerBookG4-17-di-Pietro-Arico.local ([147. } 163.124.32]) } 2, 18 -- by mail.unipa.it (8.13.1/8.13.1) with ESMTP id m4SFL2ox019466 } 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 17:21:07 +0200 } 2, 18 -- Message-ID: {483D785C.2010207-at-email.it} } 2, 18 -- Date: Wed, 28 May 2008 17:21:00 +0200 } 2, 18 -- From: =?ISO-8859-15?Q?Pietro_Aric=F2?= {p.arico-at-email.it} } 2, 18 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) } 2, 18 -- MIME-Version: 1.0 } 2, 18 -- To: Microscopy-at-microscopy.com } 2, 18 -- Subject: Zeiss Axioskop - How to center objectives } 2, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 2, 18 -- Content-Transfer-Encoding: 8bit } 2, 18 -- X-Virus-Scanned: ClamAV 0.93/7213/Thu May 22 10:29:01 2008 } on mail.unipa.it } 2, 18 -- X-Virus-Status: Clean } ==============================End of - Headers==============================
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Dear Melina, I use a hot glue gun to stick my samples to the stubs, since I have found in the past that carbon paint is not always a reliable glue. Just paint over the glue with a bit of carbon paint, after the glue is set. Regards,
-----Original Message----- X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae] Sent: Tuesday, May 27, 2008 10:51 PM To: MARDINLY, A
Hello,
How do you dry carbon paints fast? I normally glue my rock samples a day prior to imaging them. However, some wants to image them right away when I receive their specimens. Any recommendation of a good "dryer"??
Thanks.
Melina Miralles PGSc Lab Technician The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 17, 34 -- From maryflet-at-interchange.ubc.ca Wed May 28 11:48:02 2008 17, 34 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca [137.82.45.15]) 17, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SGm1e0017074 17, 34 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 11:48:02 -0500 17, 34 -- X-Ubc-Received: from mr8.mail-relay.ubc.ca (localhost [127.0.0.1]) 17, 34 -- by localhost (Postfix) with SMTP id 5348710A36 17, 34 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 09:48:01 -0700 (PDT) 17, 34 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 17, 34 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP 17, 34 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 09:47:59 -0700 (PDT) 17, 34 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 17, 34 -- by smtp.interchange.ubc.ca 17, 34 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 17, 34 -- with ESMTP id {0K1L004PH7ZYOJ-at-smtp.interchange.ubc.ca} for 17, 34 -- microscopy-at-microscopy.com; Wed, 28 May 2008 09:47:59 -0700 (PDT) 17, 34 -- Date: Wed, 28 May 2008 09:47:22 -0700 17, 34 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 17, 34 -- Subject: 17, 34 -- To: microscopy-at-microscopy.com 17, 34 -- Reply-to: maryflet-at-interchange.ubc.ca 17, 34 -- Message-id: {0K1L004PI7ZYOJ-at-smtp.interchange.ubc.ca} 17, 34 -- Organization: Materials Eng. 17, 34 -- MIME-version: 1.0 17, 34 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 17, 34 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 34 -- Content-type: text/plain; charset=iso-8859-1 17, 34 -- Thread-index: AcjA4n/OQP/CpWRbS2SYy/0z5M7RLg== 17, 34 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.5.28.92754 17, 34 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 17, 34 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_1300_1399 0, BODY_SIZE_5000_LESS 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_SUBJ_7 0, __USER_AGENT_MS_GENERIC 0 17, 34 -- X-Spam-Level: 17, 34 -- X-Spam-Flag: No 17, 34 -- Content-Transfer-Encoding: 8bit 17, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4SGm1e0017074 ==============================End of - Headers==============================
The 40x objective is fixed and you should start by centering the stage rotation around the center of this objective. There should be a couple of centering screws on the side of the stage (I don't know the specific design of this scope, but they should be fairly easy to access). Place a slide on the stage and get a focused image of a small feature with the 40x objective. Center the feature in the field of view, rotate the stage (taking care to not move the slide) and track the feature. The centering procedure will be the same for the stage as it would be for the objectives, but you are moving the stage relative to the 40x. objective. Once you have the stage centered, you can center the remaining objectives as you normally would.
Hope this helps, Bryan Bandli
p.arico-at-email.it wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi everybody, } I have got an old (about 80's, I think) polarizing microscope Zeiss } Axioskop (the model with analyzer inserted in a window at 45°). I have } to center the four objectives...no problem with the 2.5x, 10x, 20x...but } the 40x does not have the two screws to regulate it...how could I center it? } Pietro }
-- ~~~~~~~~~~~~~~~~~~~~ Bryan Bandli SEM Laboratory Manager University of Minnesota Duluth Life Science 93
Mail: UMD Geological Sciences 229 Heller Hall 1114 Kirby Dr. Duluth, MN 55812-3036
Phone: 218-726-7362 Fax: 218-726-8275
==============================Original Headers============================== 12, 31 -- From bbandli-at-d.umn.edu Wed May 28 12:13:57 2008 12, 31 -- Received: from mx0.d.umn.edu (mx0.d.umn.edu [131.212.109.42]) 12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SHDvL2011590 12, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 12:13:57 -0500 12, 31 -- Received: from mail.d.umn.edu (mail.d.umn.edu [131.212.109.2]) 12, 31 -- by mx0.d.umn.edu (8.13.8/8.13.8) with ESMTP id m4SHDvGV024980 12, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 12:13:57 -0500 (CDT) 12, 31 -- Received: from mxv2.d.umn.edu (mxv2.d.umn.edu [131.212.109.136]) 12, 31 -- by mail.d.umn.edu (8.12.9/8.12.9) with ESMTP id m4SHDus9013428 12, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 12:13:56 -0500 (CDT) 12, 31 -- Received: from smtp.d.umn.edu (mx3.d.umn.edu [131.212.109.40]) 12, 31 -- by mxv2.d.umn.edu (8.13.8/8.13.8) with ESMTP id m4SHDuR0005478 12, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 12:13:56 -0500 (CDT) 12, 31 -- Received: from [131.212.71.113] (nomad71-113.d.umn.edu [131.212.71.113]) 12, 31 -- (authenticated bits=0) 12, 31 -- by smtp.d.umn.edu (8.13.8/8.13.8) with ESMTP id m4SHDZTn014517 12, 31 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 12, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 12:13:35 -0500 (CDT) 12, 31 -- Message-ID: {483D92BE.5020709-at-d.umn.edu} 12, 31 -- Date: Wed, 28 May 2008 12:13:34 -0500 12, 31 -- From: Bryan Bandli {bbandli-at-d.umn.edu} 12, 31 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 12, 31 -- MIME-Version: 1.0 12, 31 -- To: Microscopy-at-microscopy.com 12, 31 -- Subject: Re: [Microscopy] Zeiss Axioskop - How to center objectives 12, 31 -- References: {200805281531.m4SFVxEW006528-at-ns.microscopy.com} 12, 31 -- In-Reply-To: {200805281531.m4SFVxEW006528-at-ns.microscopy.com} 12, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 31 -- Content-Transfer-Encoding: 8bit 12, 31 -- X-Virus-Scanned: ClamAV version 0.93, clamav-milter version 0.93 on mxv2.d.umn.edu 12, 31 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Ok, thank you all for the precious advices... I could not find the screws to center the stage!!! anyway, I moved the stage till I got the 2.5x centered, by unscrewing the 4 screws holding the stage blocked...then I centered other objectives using the normal procedure. It is very strange that there are no screws to center the stage...among other strange features of this (very good) microscope there is the analyzer...it is placed in the same place I should introduce the quartz wedge!!! so, I have got a very good microscope but I cannot use the quartz wedge! Pietro
==============================Original Headers============================== 1, 18 -- From p.arico-at-email.it Wed May 28 12:57:20 2008 1, 18 -- Received: from mail.unipa.it (mail.unipa.it [147.163.1.45]) 1, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SHvJIm026885 1, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 12:57:19 -0500 1, 18 -- Received: from PowerBookG4-17-di-Pietro-Arico.local ([147.163.124.32]) 1, 18 -- by mail.unipa.it (8.13.1/8.13.1) with ESMTP id m4SHvDaf031057 1, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 19:57:18 +0200 1, 18 -- Message-ID: {483D9CF6.9090900-at-email.it} 1, 18 -- Date: Wed, 28 May 2008 19:57:10 +0200 1, 18 -- From: =?ISO-8859-15?Q?Pietro_Aric=F2?= {p.arico-at-email.it} 1, 18 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 1, 18 -- MIME-Version: 1.0 1, 18 -- To: Microscopy-at-microscopy.com 1, 18 -- Subject: Mission: Axioskop...centered! 1, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 1, 18 -- Content-Transfer-Encoding: 7bit 1, 18 -- X-Virus-Scanned: ClamAV 0.93/7213/Thu May 22 10:29:01 2008 on mail.unipa.it 1, 18 -- X-Virus-Status: Clean ==============================End of - Headers==============================
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Email: saubut-at-nrcan.gc.ca Name: Sarah Aubut
Title-Subject: [Filtered] Digital Micrograph - resolution
Question: Hello listservers,
I have a dilemma: I have acquired images for a client on our TEM, which is equipped with a Gatan Erlangshen ES500W camera. Using Digital Micrograph, I acquired images in the DM3 format. I then batch convert to Tiff or BMP, and gave the client both the original DM3 files as well as the converted ones. I instructed them on opening the DM3 in Image J, which was not a problem. The problem is that once in Image J, they need to be converted into tiff, or other common file type for use in other programs, ie. for preparing a manuscript. My client has a problem with a "resolution loss" once the file type is changed. The image is about half the size (~1.3MB), but their main concern is that the dpi is only 72(when opened in Corel), and that they won't be accepted if they have that low of a dpi. I haven't been able to find any information on how important the dpi # is, and I'm a bit skeptical as the image looks to be the same quality as the original. However, I'm not an expert, so I thought I would throw this out to you people! My client thinks I should change the settings on the camera so the original image is larger than 3MB, but I don't even know how/if this can be done! Help Please!! Thanks! Sarah
Your image size is not determined by the dpi - that is the display size and is a function of the software and/or output device (i.e., monitor or printer). Displaying the image at 72 dpi or 300 dpi makes the image look bigger or smaller on screen but the resolution isn't changed. The resolution is determined by the 1200 x 800 pixels (or whatever the image size is in pixels).
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: saubut-at-nrcan.gc.ca [mailto:saubut-at-nrcan.gc.ca] Sent: Wednesday, May 28, 2008 2:09 PM To: Phillips, Thomas E.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both saubut-at-nrcan.gc.ca as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: saubut-at-nrcan.gc.ca Name: Sarah Aubut
Title-Subject: [Filtered] Digital Micrograph - resolution
Question: Hello listservers,
I have a dilemma: I have acquired images for a client on our TEM, which is equipped with a Gatan Erlangshen ES500W camera. Using Digital Micrograph, I acquired images in the DM3 format. I then batch convert to Tiff or BMP, and gave the client both the original DM3 files as well as the converted ones. I instructed them on opening the DM3 in Image J, which was not a problem. The problem is that once in Image J, they need to be converted into tiff, or other common file type for use in other programs, ie. for preparing a manuscript. My client has a problem with a "resolution loss" once the file type is changed. The image is about half the size (~1.3MB), but their main concern is that the dpi is only 72(when opened in Corel), and that they won't be accepted if they have that low of a dpi. I haven't been able to find any information on how important the dpi # is, and I'm a bit skeptical as the image looks to be the same quality as the original. However, I'm not an expert, so I thought I would throw this out to you people! My client thinks I should change the settings on the camera so the original image is larger than 3MB, but I don't even know how/if this can be done! Help Please!! Thanks! Sarah
1) You are not considering doing this with LM, are you?.. There are some LM stains that bring up mitochondria nicely. The one I used, } 15 years ago as a student, was Mallory's phosphotungstic hematoxylin (unless I am confusing it with something else :)). Also at the LM level, you can use antibody to COX - cytochrome oxidase. That's much more recent, COX is a legit mitochondria marker, as my kid would say...
2) For EM - cacodylate buffer, good fresh OsO4, UA treatment before embedding (2% in water 2 h or 1.5% in 70% ethanol overnight), and not too long in any of the dehydration or infiltration steps - but I know you know all this yourself...
3) For automatic tracing of mitochondria, did you, or your client, apply any smoothing/filtering? I am not familiar with Metamorph but did spend quite a bit of time trying different ways of helping software detect the edges and extract features from my EM images. Something between median filtering and non-linear anisotropic diffusion often solves the issue. Basically, these filters allow for more smoothing with less feature loss. Among free software, IMOD and ImageJ both will do these filters. They will also propagate a strong edge, e.g. from section to the next section. If you send me one of those pictures, I'll be happy to take a look.
4) For didactic value, if there are indeed students involved, nothing will beat the good old fashioned cutting out the mitochondria from a print with scissors and weighing on a balance.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} } Dear Collective, } } Is anyone aware of a method of processing/staining tissue so that } mitochondria will jump out at you in the resulting TEM images? } } One of our clients wants to use Metamorph to count and measure } areas of } mitochondria in muscle tissue and the program so far has been } unable to } separate the organelles from the surroundings. They don't want to } have } to resort to manual tracing of the structures, if they can avoid } it. So } far, I haven't been able to locate any specific stains for TEM of } mitochondria, but maybe there is something similar to silver staining } for nerve tissue? } } I have run across one reference to using acetonitrile for dehydration, } coupled with imidazole-buffered osmium for secondary fixation as a way } of emphasizing mitochondria, so I may try that. I would be interested } in any experiences someone might have had with that technique. } } I may also try conventional, as opposed to microwave, processing, as a } way of extracting more tissue components around the mitochondria as a } possible way of isolating them visually. } } Any suggestions would be greatly appreciated. } } And, hey, Happy Belated Norwegian Independence Day (May 17)! } } Randy Tindall } President, Officers, and Member } Half-Norwegian (on my mother's side) Microscopy Society of America } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl? } Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } } } ==============================Original } Headers============================== } 9, 23 -- From TindallR-at-missouri.edu Wed May 28 08:46:20 2008 } 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um- } tsmtpout1.um.umsystem.edu [209.106.228.23]) } 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m4SDkKkU008695 } 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 } 08:46:20 -0500 } 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu } ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 9, 23 -- Wed, 28 May 2008 08:46:17 -0500 } 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 23 -- Content-class: urn:content-classes:message } 9, 23 -- MIME-Version: 1.0 } 9, 23 -- Content-Type: text/plain; } 9, 23 -- charset="us-ascii" } 9, 23 -- Subject: TEM: mitochondria and image analysis } 9, 23 -- Date: Wed, 28 May 2008 08:46:17 -0500 } 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD76A0-at-UM- } XMAIL08.um.umsystem.edu} } 9, 23 -- X-MS-Has-Attach: } 9, 23 -- X-MS-TNEF-Correlator: } 9, 23 -- Thread-Topic: TEM: mitochondria and image analysis } 9, 23 -- Thread-Index: AcjAyTQZYxGo7D75Thaav+zzhHUymw== } 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 9, 23 -- To: {microscopy-at-microscopy.com} } 9, 23 -- X-OriginalArrivalTime: 28 May 2008 13:46:17.0924 (UTC) } FILETIME=[34589840:01C8C0C9] } 9, 23 -- Content-Transfer-Encoding: 8bit } 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4SDkKkU008695 } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 23 -- From vladislav_speransky-at-nih.gov Wed May 28 14:24:14 2008 10, 23 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SJOENZ004817 10, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 14:24:14 -0500 10, 23 -- X-IronPortListener: NIH_Relay 10, 23 -- X-SBRS: None 10, 23 -- X-IronPort-AV: E=Sophos;i="4.27,556,1204520400"; 10, 23 -- d="scan'208";a="18877793" 10, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 10, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 28 May 2008 15:24:14 -0400 10, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 10, 23 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m4SJOEdt16251831 10, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 15:24:14 -0400 (EDT) 10, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 10, 23 -- References: {200805281347.m4SDlHLS010082-at-ns.microscopy.com} 10, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 10, 23 -- Message-Id: {6D008CEB-C940-4039-AABD-8FC49FFDF6AC-at-nih.gov} 10, 23 -- Content-Transfer-Encoding: 7bit 10, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 10, 23 -- Subject: Fwd: [Microscopy] TEM: mitochondria and image analysis 10, 23 -- Date: Wed, 28 May 2008 15:24:02 -0400 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
Digital imaging has so many things controlled by various things from the aquisition to the final output. Like Tom said, the image resolution is set by the pixel resolution at aquisition time. But ugly things can subsequently happen. A new document in Corel is typically 72 dpi by default unless changed; the assumption is that you are developing images for computer viewing and computer monitors are approx 72 dpi. Now if you drop an image onto a page at that default resolution you have to grab the "handles" and drag it to the desired size on the page. Here is the problem: when you click "Apply" (in Corel9....) it resamples the image to the 72 dpi page resolution and the original resolution is gone. If you had started with a page at 300dpi (near photographic) then an 1800pixel wide image is 6" wide on the 300dpi page and if printed on a good 300dpi printer (true 300dpi like a dye sublimation printer, or equivalent).
I think that if an image has no scale data in the tag fields (TIFF) Corel will treat it as a 72dpi image by default so the hypothetical 1800pixel-wide image is (1800/72)inches in hypothetical size.
There is wealth of information about digital imaging on the Molecular Expressions website: http://micro.magnet.fsu.edu/primer/digitalimaging/index.html
Dale Callaham Umass -at- Amherst
saubut-at-nrcan.gc.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both saubut-at-nrcan.gc.ca as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: saubut-at-nrcan.gc.ca } Name: Sarah Aubut } } Title-Subject: [Filtered] Digital Micrograph - resolution } } Question: Hello listservers, } } I have a dilemma: I have acquired images for a client on our TEM, } which is equipped with a Gatan Erlangshen ES500W camera. Using } Digital Micrograph, I acquired images in the DM3 format. I then batch } convert to Tiff or BMP, and gave the client both the original DM3 } files as well as the converted ones. I instructed them on opening the } DM3 in Image J, which was not a problem. The problem is that once in } Image J, they need to be converted into tiff, or other common file } type for use in other programs, ie. for preparing a manuscript. My } client has a problem with a "resolution loss" once the file type is } changed. The image is about half the size (~1.3MB), but their main } concern is that the dpi is only 72(when opened in Corel), and that } they won't be accepted if they have that low of a dpi. I haven't been } able to find any information on how important the dpi # is, and I'm a } bit skeptical as the image looks to be the same quality as the } original. However, I'm not an expert, so I thought I would throw this } out to you people! My client thinks I should change the settings on } the camera so the original image is larger than 3MB, but I don't even } know how/if this can be done! } Help Please!! } Thanks! } Sarah } } Login Host: 198.103.92.143 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Wed May 28 14:08:07 2008 } 6, 11 -- Received: from [10.22.2.162] (msdvpn8.msd.anl.gov [130.202.238.72]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SJ85DP011231 } 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 14:08:06 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240805c4635e05f8b6-at-[10.22.2.162]} } 6, 11 -- Date: Wed, 28 May 2008 14:08:04 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: saubut-at-nrcan.gc.ca (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Digital Micrograph - resolution } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 22 -- From dac-at-research.umass.edu Wed May 28 15:05:07 2008 7, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SK57fb019749 7, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 15:05:07 -0500 7, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 22 -- (authenticated bits=0) 7, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m4SK57RX010825 7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 16:05:07 -0400 7, 22 -- Message-ID: {483DC967.8060102-at-research.umass.edu} 7, 22 -- Date: Wed, 28 May 2008 16:06:47 -0500 7, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 22 -- Reply-To: dac-at-research.umass.edu 7, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 7, 22 -- MIME-Version: 1.0 7, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 22 -- Subject: Re: [Microscopy] viaWWW: Digital Micrograph - resolution 7, 22 -- References: {200805281914.m4SJE0lf019769-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {200805281914.m4SJE0lf019769-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
A long time since I have done any enzyme cytochemistry but some of these old techniques may help.
Test for cytochrome oxidase maybe, or Glutamic oxaloacetic transaminase. There are quite a range of mitochondria enzymes that may possibly be useful
Some of these tests were lead based, some cerium chloride. Others used DAB.
There is a book called Electron Microscopic Cytochemsitry and Immunocytochemistry in BioMedicine by Ogawa and Barka and also one of the Glauert series by Lewis and Knight (Cytochemical staining methods for electron microscopy) that had methods.
Regards
Allan Mitchell
On 29/05/2008, at 1:46 AM, TindallR-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Collective, } } Is anyone aware of a method of processing/staining tissue so that } mitochondria will jump out at you in the resulting TEM images? } } One of our clients wants to use Metamorph to count and measure } areas of } mitochondria in muscle tissue and the program so far has been } unable to } separate the organelles from the surroundings. They don't want to } have } to resort to manual tracing of the structures, if they can avoid } it. So } far, I haven't been able to locate any specific stains for TEM of } mitochondria, but maybe there is something similar to silver staining } for nerve tissue? } } I have run across one reference to using acetonitrile for dehydration, } coupled with imidazole-buffered osmium for secondary fixation as a way } of emphasizing mitochondria, so I may try that. I would be interested } in any experiences someone might have had with that technique. } } I may also try conventional, as opposed to microwave, processing, as a } way of extracting more tissue components around the mitochondria as a } possible way of isolating them visually. } } Any suggestions would be greatly appreciated. } } And, hey, Happy Belated Norwegian Independence Day (May 17)! } } Randy Tindall } President, Officers, and Member } Half-Norwegian (on my mother's side) Microscopy Society of America } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl? } Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } } } ==============================Original } Headers============================== } 9, 23 -- From TindallR-at-missouri.edu Wed May 28 08:46:20 2008 } 9, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um- } tsmtpout1.um.umsystem.edu [209.106.228.23]) } 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m4SDkKkU008695 } 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 } 08:46:20 -0500 } 9, 23 -- Received: from UM-XMAIL08.um.umsystem.edu } ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 9, 23 -- Wed, 28 May 2008 08:46:17 -0500 } 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 23 -- Content-class: urn:content-classes:message } 9, 23 -- MIME-Version: 1.0 } 9, 23 -- Content-Type: text/plain; } 9, 23 -- charset="us-ascii" } 9, 23 -- Subject: TEM: mitochondria and image analysis } 9, 23 -- Date: Wed, 28 May 2008 08:46:17 -0500 } 9, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD76A0-at-UM- } XMAIL08.um.umsystem.edu} } 9, 23 -- X-MS-Has-Attach: } 9, 23 -- X-MS-TNEF-Correlator: } 9, 23 -- Thread-Topic: TEM: mitochondria and image analysis } 9, 23 -- Thread-Index: AcjAyTQZYxGo7D75Thaav+zzhHUymw== } 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 9, 23 -- To: {microscopy-at-microscopy.com} } 9, 23 -- X-OriginalArrivalTime: 28 May 2008 13:46:17.0924 (UTC) } FILETIME=[34589840:01C8C0C9] } 9, 23 -- Content-Transfer-Encoding: 8bit } 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4SDkKkU008695 } ==============================End of - } Headers==============================
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
==============================Original Headers============================== 17, 22 -- From allan.mitchell-at-stonebow.otago.ac.nz Wed May 28 15:23:19 2008 17, 22 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SKNHM8001017 17, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 28 May 2008 15:23:18 -0500 17, 22 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 17, 22 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id m4SKNDdG001857; 17, 22 -- Thu, 29 May 2008 08:23:14 +1200 17, 22 -- Received: from allan.otago.ac.nz ([139.80.40.92]) 17, 22 -- by galadriel.otago.ac.nz with esmtp (Exim 4.63) 17, 22 -- (envelope-from {allan.mitchell-at-stonebow.otago.ac.nz} ) 17, 22 -- id 1K1SAz-0000UX-4A; Thu, 29 May 2008 08:23:13 +1200 17, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 17, 22 -- In-Reply-To: {200805281346.m4SDkWXw008895-at-ns.microscopy.com} 17, 22 -- References: {200805281346.m4SDkWXw008895-at-ns.microscopy.com} 17, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 17, 22 -- Message-Id: {8B03A1BB-A8E4-4697-B794-737DC54977B7-at-stonebow.otago.ac.nz} 17, 22 -- Content-Transfer-Encoding: 7bit 17, 22 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 17, 22 -- Subject: Re: [Microscopy] TEM: mitochondria and image analysis 17, 22 -- Date: Thu, 29 May 2008 08:23:13 +1200 17, 22 -- To: TindallR-at-missouri.edu, microscopy-at-msa.microscopy.com 17, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
Another thing that might be related to the reduced file size is the bit depth. Image acquired from the CCD camera is likely being 16 or 32 bit. But it has to be converted to 8 bit TIFF file in order to be displayed properly in normal softwares such as Windows Image Viewer or MS-Word. This process will cause some information loss and reduce the file size.
Digital imaging has so many things controlled by various things from the aquisition to the final output. Like Tom said, the image resolution is set by the pixel resolution at aquisition time. But ugly things can subsequently happen. A new document in Corel is typically 72 dpi by default unless changed; the assumption is that you are developing images for computer viewing and computer monitors are approx 72 dpi. Now if you drop an image onto a page at that default resolution you have to grab the "handles" and drag it to the desired size on the page. Here is the problem: when you click "Apply" (in Corel9....) it resamples the image to the 72 dpi page resolution and the original resolution is gone. If you had started with a page at 300dpi (near photographic) then an 1800pixel wide image is 6" wide on the 300dpi page and if printed on a good 300dpi printer (true 300dpi like a dye sublimation printer, or equivalent).
I think that if an image has no scale data in the tag fields (TIFF) Corel will treat it as a 72dpi image by default so the hypothetical 1800pixel-wide image is (1800/72)inches in hypothetical size.
There is wealth of information about digital imaging on the Molecular Expressions website: http://micro.magnet.fsu.edu/primer/digitalimaging/index.html
Dale Callaham Umass -at- Amherst
saubut-at-nrcan.gc.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both saubut-at-nrcan.gc.ca as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: saubut-at-nrcan.gc.ca } Name: Sarah Aubut } } Title-Subject: [Filtered] Digital Micrograph - resolution } } Question: Hello listservers, } } I have a dilemma: I have acquired images for a client on our TEM, } which is equipped with a Gatan Erlangshen ES500W camera. Using } Digital Micrograph, I acquired images in the DM3 format. I then batch } convert to Tiff or BMP, and gave the client both the original DM3 } files as well as the converted ones. I instructed them on opening the } DM3 in Image J, which was not a problem. The problem is that once in } Image J, they need to be converted into tiff, or other common file } type for use in other programs, ie. for preparing a manuscript. My } client has a problem with a "resolution loss" once the file type is } changed. The image is about half the size (~1.3MB), but their main } concern is that the dpi is only 72(when opened in Corel), and that } they won't be accepted if they have that low of a dpi. I haven't been } able to find any information on how important the dpi # is, and I'm a } bit skeptical as the image looks to be the same quality as the } original. However, I'm not an expert, so I thought I would throw this } out to you people! My client thinks I should change the settings on } the camera so the original image is larger than 3MB, but I don't even } know how/if this can be done! } Help Please!! } Thanks! } Sarah } } Login Host: 198.103.92.143 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Wed May 28 14:08:07 2008 } 6, 11 -- Received: from [10.22.2.162] (msdvpn8.msd.anl.gov [130.202.238.72]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SJ85DP011231 } 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 28 May 2008 14:08:06 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240805c4635e05f8b6-at-[10.22.2.162]} } 6, 11 -- Date: Wed, 28 May 2008 14:08:04 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: saubut-at-nrcan.gc.ca (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Digital Micrograph - resolution } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 22 -- From dac-at-research.umass.edu Wed May 28 15:05:07 2008 7, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SK57fb019749 7, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 15:05:07 -0500 7, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 7, 22 -- (authenticated bits=0) 7, 22 -- by race3.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m4SK57RX010825 7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 16:05:07 -0400 7, 22 -- Message-ID: {483DC967.8060102-at-research.umass.edu} 7, 22 -- Date: Wed, 28 May 2008 16:06:47 -0500 7, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 7, 22 -- Reply-To: dac-at-research.umass.edu 7, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 7, 22 -- MIME-Version: 1.0 7, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 22 -- Subject: Re: [Microscopy] viaWWW: Digital Micrograph - resolution 7, 22 -- References: {200805281914.m4SJE0lf019769-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {200805281914.m4SJE0lf019769-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
==============================Original Headers============================== 14, 23 -- From lixxx326-at-umn.edu Wed May 28 15:39:05 2008 14, 23 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 14, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4SKd5C3014366 14, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 28 May 2008 15:39:05 -0500 14, 23 -- Received: from universitf2v1c (x-128-101-160-221.chem.umn.edu [128.101.160.221]) 14, 23 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 14, 23 -- Wed, 28 May 2008 15:39:01 -0500 (CDT) 14, 23 -- X-Umn-Remote-Mta: [N] x-128-101-160-221.chem.umn.edu [128.101.160.221] #+LO+TS+AU+HN 14, 23 -- From: "Fan Li" {lixxx326-at-umn.edu} 14, 23 -- To: {Microscopy-at-microscopy.com} 14, 23 -- Cc: {saubut-at-nrcan.gc.ca} , {lixxx326-at-umn.edu} 14, 23 -- References: {200805282012.m4SKCUkd032711-at-ns.microscopy.com} 14, 23 -- In-Reply-To: {200805282012.m4SKCUkd032711-at-ns.microscopy.com} 14, 23 -- Subject: RE: [Microscopy] Re: viaWWW: Digital Micrograph - resolution 14, 23 -- Date: Wed, 28 May 2008 15:34:51 -0500 14, 23 -- Message-ID: {009101c8c102$47bdb710$d7392530$-at-edu} 14, 23 -- MIME-Version: 1.0 14, 23 -- Content-Type: text/plain; 14, 23 -- charset="us-ascii" 14, 23 -- Content-Transfer-Encoding: 7bit 14, 23 -- X-Mailer: Microsoft Office Outlook 12.0 14, 23 -- Thread-Index: AcjA/ynEzE4ydEKZQUeoySXsHGL/QAAAdqYg 14, 23 -- Content-Language: en-us ==============================End of - Headers==============================
They are excellent for starting ion pumps. Especially Amray Varian ones that will not start (IPG in particular).
gary g.
At 09:17 AM 5/28/2008, you wrote:
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I saw this interesting story in the NY Times that Sumio Iijima, long regarded as one of the emminent microscopists in the world, was chosen as one of the recipients of the Kavli Prize. The Kavli Prizes are worth $1 million each in the fields of astrophysics, nanoscience and neuroscience. The awards were established by the Norwegian-born physicist, businessman and philanthropist Fred Kavli. The full story is at:
Dear All, Diaspro Lab proudly reminds about the Microscopy event that will be held at Department of Physics, Via Dodecaneso 33, Genova.
Workshop on "Aspetti pratici di Microscopia Multifotonica e Nanoscopia", Genova 3 Giugno 2008. Information at http://www.sism.it/scuole_eventi.php, brochure at Events - http://www.lambs.it.
All the best AD
--------------------------------------------------------- Alberto Diaspro, MicroScoBio - Department of Physics University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy facsimile +39-010314218 - voice +39-0103536426/480/309 URL: http://www.lambs.it www.darwinweb.it http://www.wiley.com/WileyCDA/WileyTitle/productCd-0471409200.html ---------------------------------------------------------
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Email: ebeniash-at-pitt.edu Name: Elia Beniash
Organization: University of Pittsburgh
Title-Subject: [Filtered] postdoctoral position open
Question: Dear subscribers, I am currently looking for a person with an experience in materials TEM interested in doing postdocoral research in a field of biomineralals. This project is focused on the early events of tooth formation and specifically the the interface between dentin and enamel. The candidate will study the early stages of mineral formation using TEM, FTIR microspectroscopy and other characterization techniques You can contact me at the address below, sincerely Elia Beniash
Elia Beniash, PhD Associate Professor Oral Biology; University of Pittsburgh School of Dental Medicine Bioengineering; University of Pittsburgh School of Engineering Center for Craniofacial Regeneration McGowan Institute for Regenerative Medicine 589 Salk Hall, 3501 Terrace Street Pittsburgh, PA 15261 Tel. (1) 412 6480108 Fax (1) 412 6246685 Email: ebeniash-at-pitt.edu
The problem with trying to put a Farday cup into the end of a TEM specimen rod is that most specimen rods are too thin to accommodate a good one. The backscattering of electrons is the basic problem here. For a Faraday cup to be really effective it needs to be one or two centimeters deep in order to trap all the incident electrons, otherwise a good fraction of them are lost by being backscattered away and you don't get an accurate reading of the beam current anyway. Unfortunately, in order for the specimen rod in most the TEMs to fit between the objective lens pole pieces where it can intercept the electron beam it can only be one or two millimeters thick, and this would not allow the accommodation of a truly effective Faraday cup.
An alternative, and probably less expensive and more effective approach, is to design an independent Faraday cup to be installed through a port in the viewing chamber. I designed one such device for a Philips EM several years ago, and as far as I know it worked out very satisfactorily. If anyone is interested I will be happy to give them more details. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
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The use of carbon paint to mount specimen-carrying stubs in a scanning electron microscope is a potential source for a serious vacuum problem, as described on page 76 of my book, "Vacuum Methods in Electron Microscopy". Often the user smears the entire under surface of the specimen stub with a thick coating of carbon paint, places it on the microscope stage, waits a few minutes until the visible ring of paint around the bottom edge of the stub appears to be dry (assumes all of it is dry) and proceeds to pump the instrument out. What usually happens then is that this user comes around complaining that the SEM's vacuum system is not functioning properly because it is taking an abnormally long time to pump down.
What happens in a situation like this is that whereas the visible ring of carbon paint around the bottom edge of the stub appears to be dry, the large quantity of paint underneath the stub remains un-dry, and the solvent that it contains is slowly diffusing out through the "dry ring" causing the rate of evacuation to slow down markedly.
A better approach is to set the stub on the stage dry, and then to place a few small isolated dabs of carbon paint around the bottom edge of it. Three or four such small dabs will dry quickly, and if allowed to become really and truly dry, will usually hold most stubs in firmly place. There will then be no trapped paint to bleed solvent into the vacuum system, and operation should proceed smoothly in a normal manner. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
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Email: pinhead01-at-msn.com Name: Pierre Bustanoby
Organization: The Boeing Co.
Title-Subject: [Filtered] Sawtooth edges of Sem Images
Question: Marissa / Et al:
The saw toothed image edges that you are dealing with are most likely caused by mechanical vibration. A quick way to verify this is to go to the fastest rapid scan and see if the image is wavering or looks like it is under water. Soft tapping on the chamber should result in increasing this wavering.
Some of the things to check for are:
1) Loose sample on holder.
2) Excessive mechanical slop in either X, Y or Z stage mechanics.
3) If the stage has a mechanical vibration snubber at the back of the chamber check to see that it firmly holds the stage stable.
4) Electrical interference usually results in symmetric banding due to its periodic nature and probably isn't causing your jagged edges.
Good Luck! Pierre Bustanoby Electron/Ion Optics Repair Boeing Aircraft Seattle WA.
What we all must understand here is that as the image is made up of lines there are a wide array of faults that will cause an edge to be jagged, some due to instrument problems, some due to external influences.
Set out below a couple of simple tests that will isolate the most common of these faults. What we are trying to accomplish is to determine if the fault is due to a magnetic field or vibration. A simple jagged edge is rarely due to charge and discharge, this results in a darkening of the image as it scans down and then a bright line or line as it discharges.
1. Long working distance (25-30mm) does the problem become greater? 2. Short working distance (5-7mm) does the problem become less?
Or
3. Low kV (5) does the problem become greater? 4. High kV (30) does the problem become less?
Each of these series proves the field problem if you have a change.
Third test
I have rotated a column 45 degrees, the question being is the problem the same or does it change? A change in physical orientation providing a change in the problem indicates it is external as internal (microscope) faults will stay exactly the same.
Good luck
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {pinhead01-at-msn.com} To: {protrain-at-emcourses.com} Sent: Friday, May 30, 2008 4:00 AM
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Title-Subject: [Filtered] request for very old papers
Question: Hi All, Please if anyone can find the following journal articles/ copy pages of the books please can you hunt it for me send either a hard/soft copy?
Ziegenspeck, H. (1949). Die Emission polarisierten Fluoreszenzlichtes (Difluoreszenz) durch gef”rbte Zellulose und Kutinmembranen von Pflanzen. In F. Bra?utigam & A. Grabner (Eds.), Beitrage zur Fluoreszenzmikroskopie (pp. 71-85). Wien : Fromme. (Special issue of "Mikroskopie")
Isings, J. (1966). Combined fluorescence dichroism and polarization microscopy of cotton fibres under stress. Microscope and Crystal Front (Carshalton Beeches (Surrey)), 15(2), 71-79
Luhan M, 1947. Die Goldendodermis der Farne. Fluoreszenmikroskopische untersuchungen zur vergleichenden Anatomie der Filicineen. Sitzungsberichte der Osterreichische Akademie der Wissenschaften Abteilung I. 156, 1-56.
Klein G and H Linser. 1930. Fluoreszenzanalytische untersuchungen an pflanze. Osterreische botanische zeitschrift. 79, 125-163.
I will be using the papers solely for my research/study purppose only and will not be distributing, if there is any copyright issues.
Can't see a reply to your query, so I'll write one. The Bio-Rad MRC 1024 is no longer fully supported by Zeiss CellScience (a division of Zeiss microscopes, who purchased the UK confocal production wing from Bio-Rad a few years ago), but they still repair, supply spare parts and will have all the details on the Bio-Rad 1024 confocal should you need it.
That said, the DF 620/32 is most probably a narrow band pass filter with the peak emission at 620nm and the peak width 32nm (i.e. 620 nm +/- 16 nm) - this is the case with the newer Bio-Rad Radiance 2000 HQ filter sets anyway. Regarding your 'DF's (Diochroic Filter?), I honestly can't remember back to the days of our MRC 1024 though, and no radiance filter has the DF suffix, so check this with Zeiss just in case. The OG 515 is a mystery to me (the 515 is likely to be 515 nm). I expect it's not a Bio-Rad part but is the Schott OG515 filter and thus another long pass [LP] from 515nm:
http://www.galvoptics.fsnet.co.uk/og515.htm
As you say LP is normally a Bio-Rad long pass filter, so a 585LP blocks light below 585 and lets everything above 585nm pass through. Likewise Bio-Rad narrow band filters are typically denoted by the 515/30 bit (peak 515nm +/- 15nm). The Bio-Rad 1024 manual should detail all the filter sets supplied by Bio-Rad and I'm sure Zeiss CellScience will email you it as a pdf as soon as asked:
http://www.zeiss.de/cellscience
The Bio-Rad 1024 confocal we had here lives on elsewhere in Oxford, so I've lost contact with our 1024 manuals.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
Sent: 28 May 2008 03:41 To: kjmorris-at-well.ox.ac.uk
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Email: antoniomiguel.garcia-at-gmail.com Name: Antonio
I am using a confocal microscope BIORAD MRC 1024 for fluorescence imaging. My question is a simple one, the emission filters are named things like DF620/32, OG515 or 585LP. I think DF620/32 is a band pass filter (although I cannot make up what DF stands for), and 585LP a long pass, but what is OG515?
Isings, J. (1966). Combined fluorescence dichroism and polarization microscopy of cotton fibres under stress. Microscope and Crystal Front (Carshalton Beeches (Surrey)), 15(2), 71-79
In my library at the office. I'll be out next week but will pull it the following week. If you need it sooner, call the McCrone Research Institute in Chicago, IL. They'll also have a copy.
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-----Original Message----- X-from: javahr01-at-student.uwa.edu.au [mailto:javahr01-at-student.uwa.edu.au] Sent: Friday, May 30, 2008 6:42 AM To: ph2-at-sprynet.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both javahr01-at-student.uwa.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] request for very old papers
Question: Hi All, Please if anyone can find the following journal articles/ copy pages of the books please can you hunt it for me send either a hard/soft copy?
Ziegenspeck, H. (1949). Die Emission polarisierten Fluoreszenzlichtes (Difluoreszenz) durch gef"rbte Zellulose und Kutinmembranen von Pflanzen. In F. Bra?utigam & A. Grabner (Eds.), Beitrage zur Fluoreszenzmikroskopie (pp. 71-85). Wien : Fromme. (Special issue of "Mikroskopie")
Isings, J. (1966). Combined fluorescence dichroism and polarization microscopy of cotton fibres under stress. Microscope and Crystal Front (Carshalton Beeches (Surrey)), 15(2), 71-79
Luhan M, 1947. Die Goldendodermis der Farne. Fluoreszenmikroskopische untersuchungen zur vergleichenden Anatomie der Filicineen. Sitzungsberichte der Osterreichische Akademie der Wissenschaften Abteilung I. 156, 1-56.
Klein G and H Linser. 1930. Fluoreszenzanalytische untersuchungen an pflanze. Osterreische botanische zeitschrift. 79, 125-163.
I will be using the papers solely for my research/study purppose only and will not be distributing, if there is any copyright issues.
While access to an SEM (I assume it's a scanning electron microscope) is very nice, you still can't compete with a decent book full of pretty (SEM) pictures. Have a look at:
Microscopic Life in the Home (Micro World) (Library Binding) by Brian R. Ward (Author) It's for primary school kids (age 7 to 10).
Unseen Companions: Big Views of Tiny Creatures (Hardcover) by Adrian Warren (Author), David Spears (Photographer), Madeleine Spears (Photographer) The same but aimed at adults and secondary school kids (10+). A great book and pretty much just what you want.
Inside the Body: Fantastic Images from Beneath the Skin (Hardcover) by Susan Greenfield (Foreword) Not the little beasties, but what we ourselves look like. Good companion to the one above.
If you can't afford to buy the books, get them from the library.
Hopefully most of the youngsters in the class won't have moose ticks, fleas or deer mites crawling all over them (or even the ever popular human head louse). And if they do, it's best not to point it out to the rest of the class. Mostly it would be bacteria on skin and unlike insects they don't take to SEM microscopy as easily having no convenient hard exoskeleton (but it can be done). Again books and the web are likely to be the best source of SEM and histological images of medically important bacteria, e.g.
These days I am pretty exclusively optical microscope not SEM/TEM, but check out the links on our web site: http://www.well.ox.ac.uk/cytogenetics/websites.shtml
e.g. Under Microscopy Theory and Related Sites:
Microscopy UK - thousands of microscopy related pages for enthusiasts http://www.microscopy-uk.org.uk
101Science.com's microscope pages for older school-kids & parents http://www.101science.com/Microscope.htm
The Science for Fun website for older school-kids & parents http://www.funsci.com/texts/index_en.htm
The McCrone atlas of microscopic particles - many images are pay to view http://www.mccroneatlas.com
Klaus Kemp's diatom & butterfly scale microscopy slides - to purchase http://www.diatoms.co.uk
I would think that looking for dust mites would be a good option. These don't actually live on us, but they do feed on our dead skin (so they are not parasites). You can easily see them under an optical microscope, which would compare well with the SEM view. Plus looking at one arthropod species under SEM will really bring to life the book images of other, more medically nasty, animals. SEM views of just an eyelash, dead skin and hair (with or without bacteria) and say clothing or money spiders (tiny spiders) would be of interest to the kids anyway - although the little spiders are just climbing up us to jump off to pastures new. We get corn thrips by the hundreds as well about now (just really tickly when they clamber over us). You can simply collect dust falling onto a glass slide (or SEM equivalent) over for a few hours, inside a house/bedroom/kitchen and see what turns up, or use a vacuum cleaner [filter] as described:
http://www.funsci.com/fun3_en/dust/dust.htm
Plus you can use fine quality artist brushes to sweep dust (and dust mites) onto the viewing surface (say a glass-slide/Petri dish and on then on to an SEM stub eventually). With plastic Petri dishes watch out for static (try breathing on the outside first). I never use sticky tape as I view using under an optical microscope, but for SEM it should be OK (chat to your SEM department guys). For hairs and stuff a sticky SEM stub should be fine if you drop them on (they can give you some with the correct sticky pad on it). Mostly collect gently, let gravity help, and avoid squishing. With bioaerosols (bacteria/fungi) I always used expensive filtration or impaction devices, but again hospitals used to just leave an open Petri dish + media out in the ward and culture on whatever dropped in. Human skins fairly greasy and most biopsies are concerned with culturing the pathogen or crime forensics, rather than looking at pretty pictures (although with dry and very dead mummies, skin is more easily obtained). People do still take cheek cell samples from the mouth but for kids it's a bit gross and there may be something nasty growing there.
You should be able to see dust mites fairly easily at 10-40x, if they are there, as they are often over 200um long - you need a decent school microscope though. You can chemically test for their presence using a chemical card. They need 70% humidity and food to thrive - so an old unused airing cupboard pillow might not provide any living specimens - perhaps try a bed mattress/pillow that's in use. Other than allergenic dust problems with their faeces & dead mites (which can be severe) they are harmless.
A 4 to 120x stereo optical microscope should also work well (or a glass-slide/compound microscope at similar mag), although dust mites are a little translucent, and they are odd looking beasties. Rather than repeat other on-line info, have a look at:
There an optical microscope image at http://strano16.interfree.it/micro03.htm
I wrote this dust mite bit a year or so ago, but the links appear to still work. How to collect the dust mites should be in the links.
Good luck.
Keith
_________________________
Dr Keith J Morris Imaging Facility Manager Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
email: keith.Morris-at-ucl.ac.uk
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: Smithcf-at-hotmail.com [mailto:Smithcf-at-hotmail.com] Sent: 28 May 2008 03:41 To: kjmorris-at-well.ox.ac.uk
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Smithcf-at-hotmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, May 27, 2008 at 15:44:03 ---------------------------------------------------------------------------
Email: Smithcf-at-hotmail.com Name: Chuck Smith
Organization: University of Connecticut
Education: 9-12th Grade High School
Location: Storrs, CT USA
Question: I'm teaching a week-long class on biodiversity this summer for middle school/high school students. I'd like one exercise to be a demonstration of biodiversity living on the human body, perhaps by having students collect samples from themselves (skin scrapings, using masking tape to lift samples, samples from carpets, etc.) and looking at these using EM. Our EM department has graciously offered their services to prepare the samples, but I'm in need of information on how and where to collect samples (eyelash mites, dust mites....). Any suggestions would be greatly appreciated.
Dear Keith, I keep forgetting that this listserver replies to the sender. In my response to Antonio: } Dear Antonio, } DF means "dichroic filter" - these are interference filters } LP means "long pass" - some are interference filters, some are } absorbance filters } OG stands for "orange glass", it is an absorbance filter made of } doped glass that absorbs all light below 515 nm. } Given the age of the 1024, you might want to take a look the } interference filters for signs of degradation that may cause } leakage of other wavelengths.
The OG515 is a standard filter still often used as a longpass filter. It was standard issue on the MRC-1000 and 1024. Ours is still in use. Filters can be ordered from Chroma, who made the originals. However, there appear to be differences in the filter mount for the PMT filter wheels, probably depending on the age of the scan head. the older MRC-1000 scanheads (our system is an 1024 upgrade on 1000) did not use threaded retainers. the filters are held in place by 2 plastic washers, one on each side of the filter, on screws threaded into the filter wheel. This is important to know when ordering new filters to specify the thickness of the mounting ring on the filter.
Regards, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
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On May 30, 2008, at 3:51 AM, kjmorris-at-well.ox.ac.uk wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Antonio, } } Can't see a reply to your query, so I'll write one. The Bio-Rad MRC } 1024 is } no longer fully supported by Zeiss CellScience (a division of Zeiss } microscopes, who purchased the UK confocal production wing from Bio- } Rad a } few years ago), but they still repair, supply spare parts and will } have all } the details on the Bio-Rad 1024 confocal should you need it. } } That said, the DF 620/32 is most probably a narrow band pass filter } with the } peak emission at 620nm and the peak width 32nm (i.e. 620 nm +/- 16 } nm) - } this is the case with the newer Bio-Rad Radiance 2000 HQ filter } sets anyway. } Regarding your 'DF's (Diochroic Filter?), I honestly can't remember } back to } the days of our MRC 1024 though, and no radiance filter has the DF } suffix, } so check this with Zeiss just in case. The OG 515 is a mystery to } me (the } 515 is likely to be 515 nm). I expect it's not a Bio-Rad part but } is the } Schott OG515 filter and thus another long pass [LP] from 515nm: } } http://www.galvoptics.fsnet.co.uk/og515.htm } } As you say LP is normally a Bio-Rad long pass filter, so a 585LP } blocks } light below 585 and lets everything above 585nm pass through. Likewise } Bio-Rad narrow band filters are typically denoted by the 515/30 bit } (peak } 515nm +/- 15nm). The Bio-Rad 1024 manual should detail all the } filter sets } supplied by Bio-Rad and I'm sure Zeiss CellScience will email you } it as a } pdf as soon as asked: } } http://www.zeiss.de/cellscience } } The Bio-Rad 1024 confocal we had here lives on elsewhere in Oxford, } so I've } lost contact with our 1024 manuals. } } Regards } } Keith } } } ---------------------------------------------------------------------- } ----- } Dr Keith J. Morris } Molecular Cytogenetics and Microscopy Core } Laboratory 00/069 and 00/070 } The Wellcome Trust Centre for Human Genetics } Roosevelt Drive } Oxford } OX3 7BN } United Kingdom } Telephone: +44 (0)1865 287568 } Email: kjmorris-at-well.ox.ac.uk } Web-pages: http://www.well.ox.ac.uk/cytogenetics/ } } -----Original Message----- } X-from: antoniomiguel.garcia-at-gmail.com } [mailto:antoniomiguel.garcia-at-gmail.com] } } Sent: 28 May 2008 03:41 } To: kjmorris-at-well.ox.ac.uk } Subject: [Microscopy] viaWWW: Emission filter nomenclature } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both antoniomiguel.garcia-at-gmail.com as well as the } MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: antoniomiguel.garcia-at-gmail.com } Name: Antonio } } Organization: UAM } } Title-Subject: [Filtered] Emission filter nomenclature } } Question: Hello! } } I am using a confocal microscope BIORAD MRC 1024 for fluorescence } imaging. My question is a simple one, the emission filters are named } things like DF620/32, OG515 or 585LP. I think DF620/32 is a band pass } filter (although I cannot make up what DF stands for), and 585LP a } long pass, but what is OG515? } } Thank you for any reply. } } } } Login Host: 150.244.74.247 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 10, 11 -- From zaluzec-at-microscopy.com Tue May 27 21:26:39 2008 } 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id } m4S2Qd5x026222 } 10, 11 -- for {microscopy-at-microscopy.com} ; Tue, 27 May 2008 21:26:39 } -0500 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240801c4627350cb3a-at-[206.69.208.22]} } 10, 11 -- Date: Tue, 27 May 2008 21:26:38 -0500 } 10, 11 -- To: microscopy-at-microscopy.com } 10, 11 -- From: antoniomiguel.garcia-at-gmail.com (by way of } MicroscopyListserver) } 10, 11 -- Subject: viaWWW: Emission filter nomenclature } 10, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 26, 22 -- From kjmorris-at-well.ox.ac.uk Fri May 30 05:50:15 2008 } 26, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 26, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m4UAoEB8024507 } 26, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 } 05:50:14 -0500 } 26, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 26, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 26, 22 -- id 1K22Ba-0007SF-85; Fri, 30 May 2008 11:50:14 +0100 } 26, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 26, 22 -- To: {antoniomiguel.garcia-at-gmail.com} } 26, 22 -- Cc: {Microscopy-at-Microscopy.Com} } 26, 22 -- References: {200805280240.m4S2efjQ004669-at-ns.microscopy.com} } 26, 22 -- Subject: RE: [Microscopy] viaWWW: Emission filter } nomenclature } 26, 22 -- Date: Fri, 30 May 2008 11:52:24 +0100 } 26, 22 -- Message-ID: {001801c8c243$3f393410$b22c4381-at-CytoWhizz} } 26, 22 -- MIME-Version: 1.0 } 26, 22 -- Content-Type: text/plain; } 26, 22 -- charset="us-ascii" } 26, 22 -- Content-Transfer-Encoding: 7bit } 26, 22 -- X-Mailer: Microsoft Office Outlook 11 } 26, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 26, 22 -- Thread-Index: AcjAbDlGNIhI50WOSQmAc+1JmKuhZwBxWQ4g } 26, 22 -- In-Reply-To: {200805280240.m4S2efjQ004669-at-ns.microscopy.com} } ==============================End of - } Headers==============================
Thanks to everyone who responded my question on emphasizing mitochondria for TEM image analysis. Lots of stuff to try now. I also found some interesting ideas in Hayat's book on Positive Staining Techniques----one involved an odd protocol using glutaraldehyde fixation followed by a three-hour stain in 5% phosphotungstic acid in 6.25% Na2SO4, followed by dehyration in acidic ethanol. I'm giving that a whirl now. Finally there is an 1992 article on using acetonitrile as the dehydrating solvent as a way of emphasizing mitochondria (Microscopy Research and Technique, Vol 21: 39-50).
Here are the replies, in all their glory: ___________________________________________
A long time since I have done any enzyme cytochemistry but some of these old techniques may help.
Test for cytochrome oxidase maybe, or Glutamic oxaloacetic transaminase. There are quite a range of mitochondria enzymes that may possibly be useful
Some of these tests were lead based, some cerium chloride. Others used DAB.
There is a book called Electron Microscopic Cytochemsitry and Immunocytochemistry in BioMedicine by Ogawa and Barka and also one of the Glauert series by Lewis and Knight (Cytochemical staining methods for electron microscopy) that had methods.
Allan Mitchell ------------------------------------------------------------------------ --
Just a thought that is really off the wall - but what about doing it with immunohistochemistry. I've done the immunoEM with DAB in monolayers infected with chlamydia with pre-embedding and it worked well. The basic concept is the same, just a different Ab for a natural occuring enzyme. It may work on LR White embedded muscle tissue. But then again, it may not.
My biochemistry days are far behind, so I am not sure what particular enzyme you would be best to stain for, but I do remember that there are some very specific enzymes for mitochondria, and believe there were some light microscopy tests.
On the other hand, as far as hand tracing, what does the client have students for? ;-)
Paul Hazelton ------------------------------------------------------------------------ ---
1) You are not considering doing this with LM, are you?.. There are some LM stains that bring up mitochondria nicely. The one I used, } 15 years ago as a student, was Mallory's phosphotungstic hematoxylin (unless I am confusing it with something else :)). Also at the LM level, you can use antibody to COX - cytochrome oxidase. That's much more recent, COX is a legit mitochondria marker, as my kid would say...
2) For EM - cacodylate buffer, good fresh OsO4, UA treatment before embedding (2% in water 2 h or 1.5% in 70% ethanol overnight), and not too long in any of the dehydration or infiltration steps - but I know you know all this yourself...
3) For automatic tracing of mitochondria, did you, or your client, apply any smoothing/filtering? I am not familiar with Metamorph but did spend quite a bit of time trying different ways of helping software detect the edges and extract features from my EM images. Something between median filtering and non-linear anisotropic diffusion often solves the issue. Basically, these filters allow for more smoothing with less feature loss. Among free software, IMOD and ImageJ both will do these filters. They will also propagate a strong edge, e.g. from section to the next section. If you send me one of those pictures, I'll be happy to take a look.
4) For didactic value, if there are indeed students involved, nothing will beat the good old fashioned cutting out the mitochondria from a print with scissors and weighing on a balance.
Do you have "Theory and Practiced of Histological Techniques" by Bancroft and Stevens, 5th edition? On page 350 it discusses a technique to visualize mitochondria with aniline-acid fuchsin, called "Altmann's technique for mitochondria".
Page 612 discusses NADH diaphorase to demonstrate mitochondria on unfixed cryostat sections. Mostly used for muscle enzyme histochemistry.
Rhonda Allen --------------------------------------------------------------------
It is really difficult to use MetaMorph to analyze things that are not fluorescently labelled, or can otherwise be isolated by setting intensity threshholds...that's how it was designed. Its meant for analysis of data collected in widefield or confocal fluorescence. My guess is that unless you unearth a method that will make the mitos really stand out as almost black against the rest of the muscle, your client is going to have to resort to tracing. I've got a number of clients that are also looking at mitos in EM, either isolated or in tissue. One group is primarily interested in structure under varying conditions, but another is also interested in relative numbers within skeletal muscle in a mouse model for a muscular disease. If you come across anything that will work in MM....please share!
Lee Cohen-Gould ------------------------------------------------------------------------
An acetone dehydration in conventional processing clears a lot of cytoplasm from cells vs. ethanol. Mitochondria, cytoskeletin, ER, golgi etc. all remain but the background is much lighter making the organelles stand out more. I have not tried the other suggestions that you list.
A word of caution that you may already know:
Depending on where the sections are taken from the muscle there can be several hundred% differences in mitochondrial volume/size. I am currently studying muscle cross sections (very close to cross) rather than longitudinal views as originally requested by the investigator because of this.
Interested in seeing others reply. Pat Connelly ---------------------------------------------------------------------- isn't there a mitochondria-specific fluorescence dye (mito- tracker or so) which you might use straight on the embedded tissue (whether in paraffin or cryosections or even on plastic semi-thin sections??) ?
- just for counting mito's, if appears to me that TEM is narrowing your field of view - more than necessary. Light microsopy?
- for counting the real area - huuups - then I would not trust the sections because a (single ultrathin) section might not give you an area which is really representative. And is it the area your client is interested in, or rather the VOLUME of the mito (which really counts)? and then, cryoprocessing (only) will give you a representative image of well preserved mitochondria (demanding, I know).
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 33, 24 -- From TindallR-at-missouri.edu Fri May 30 13:43:14 2008 33, 24 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 33, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UIhERH003420 33, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 13:43:14 -0500 33, 24 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 33, 24 -- Fri, 30 May 2008 13:43:14 -0500 33, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 33, 24 -- Content-class: urn:content-classes:message 33, 24 -- MIME-Version: 1.0 33, 24 -- Content-Type: text/plain; 33, 24 -- charset="us-ascii" 33, 24 -- Subject: Mitochondria query summary---Thanks!! (Long) 33, 24 -- Date: Fri, 30 May 2008 13:43:14 -0500 33, 24 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD76B0-at-UM-XMAIL08.um.umsystem.edu} 33, 24 -- X-MS-Has-Attach: 33, 24 -- X-MS-TNEF-Correlator: 33, 24 -- Thread-Topic: Mitochondria query summary---Thanks!! (Long) 33, 24 -- Thread-Index: AcjChQRq9fcHzZBUTQ+12Q6of7dHew== 33, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 33, 24 -- To: {microscopy-at-microscopy.com} 33, 24 -- Cc: "Naples, Scott Patrick (MU-Student)" {spnd36-at-mizzou.edu} 33, 24 -- X-OriginalArrivalTime: 30 May 2008 18:43:14.0401 (UTC) FILETIME=[049EBD10:01C8C285] 33, 24 -- Content-Transfer-Encoding: 8bit 33, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4UIhERH003420 ==============================End of - Headers==============================
To Apply, go to http://careers.fei.com/applyFEI2.asp?fei?fei1328-HBO?jjeske?
The Position
The PME, as part of the applications and sales team, is responsible for providing technical expertise in the support of FEI's North American sales efforts. Primary responsibilities include the following:
* Performing high quality SEM product demonstrations, including the development of new techniques and applications and product differentiators for FEI partners and customers. * Deep, technical SEM expert assisting with all phases of the sales cycle ands sales strategy. * Cultivating positive customer relationships and acting as a high level customer interface for specific instrument/applications issues, acting as a technical expert, explaining complex technology details, giving in depth presentations, and assisting in technique development. * Supporting and training customers after tool install. Assisting FEI Service Engineers on instrument sign offs and specific application techniques. * Providing continuous feedback to the product groups on system performance, features, and suggestions for improvement while collaborating with marketing/development groups on future product developments.
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This customer focused position is ideal for an experienced technologist wanting to be involved with a dynamic team and exposed to a constant variety of customer application areas. The successful candidate will possess the following combination of education and experience:
* At a minimum, BS degree in science discipline. Higher degree is greatly preferred. * 4 or more years experience in a relevant commercial/research/production establishment with recent experience utilizing SEM technology in sample characterization. * Knowledge of advanced technology FA and diagnostics as well as, operational optimization experience of SEM and various energy dispersive spectrometers or other analytical techniques required. * Excellent, enthusiastic, clear communication skills, both written and verbal, with a diverse audience is critical to the success of this position. Fluent in English. * Excellent customer interfacing and technical sales skills a must * Proven ability to interact with cross - functional and cross - cultural teams * Ability to travel both domestically as well as internationally and possession of a valid passport.
To Apply, go to http://careers.fei.com/applyFEI2.asp?fei?fei1328-HBO?jjeske?
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==============================Original Headers============================== 15, 27 -- From Resume-at-fei.com Fri May 30 14:05:25 2008 15, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UJ5PRP017133 15, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 14:05:25 -0500 15, 27 -- X-WSS-ID: 0K1P3P1-01-ZSM-01 15, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 15, 27 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 231BF96FDB5 15, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 12:05:25 -0700 (PDT) 15, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 15, 27 -- Fri, 30 May 2008 12:05:22 -0700 15, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 27 -- Content-class: urn:content-classes:message 15, 27 -- MIME-Version: 1.0 15, 27 -- Content-Type: text/plain; 15, 27 -- charset="us-ascii" 15, 27 -- Subject: Job opportunity at FEI - SEM Product Marketing Engineer 15, 27 -- Date: Fri, 30 May 2008 12:05:22 -0700 15, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB81174CD88-at-hlexc03.w2k.feico.com} 15, 27 -- X-MS-Has-Attach: 15, 27 -- X-MS-TNEF-Correlator: 15, 27 -- Thread-Topic: Job opportunity at FEI - SEM Product Marketing Engineer 15, 27 -- Thread-Index: AcjCiBvK2kDgwhfvTpqCdQyS2RpgPQ== 15, 27 -- From: "Resume" {Resume-at-fei.com} 15, 27 -- To: {Microscopy-at-Microscopy.Com} 15, 27 -- X-OriginalArrivalTime: 30 May 2008 19:05:22.0697 (UTC) FILETIME=[1C589F90:01C8C288] 15, 27 -- Content-Transfer-Encoding: 8bit 15, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4UJ5PRP017133 ==============================End of - Headers==============================
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Position Description:
The Position
Job Location: Oregon, USA The RE in the TEM applications team is responsible for providing technical expertise in the support of FEI's North American sales efforts. Primary responsibilities include the following:
* Performing high quality TEM product demonstrations, including the development of new techniques and applications for FEI partners and customers. Technical product expert assisting with all phases of the sales cycle. * Cultivating positive customer relationships and acting as a high level customer interface for specific instrument/applications issues, acting as a technical expert, explaining complex technology details, giving in depth presentations, and assisting in technique development. * Supporting and training customers after the sale has been made and the system is signed off. Assisting FEI Service Engineers on instrument sign offs and specific application techniques. * Providing continuous feedback to the product groups on system performance, features, and problems while collaborating with marketing/development groups on future product developments.
Position Requirements -
This customer focused position is ideal for an experienced technologist wanting to be involved with a dynamic team and exposed to a constant variety of customer application areas. The successful candidate will possess the following combination of education and experience:
* At a minimum, BS degree in science discipline. Higher degree is greatly preferred. * 3 or more years experience in a relevant commercial/research/production establishment with recent experience utilizing TEM technology in sample characterization. * Knowledge of advanced technology FA and diagnostics as well as, operational optimization experience of analytical equipment; specifically electron/ion microscopes and various energy dispersive spectrometers nor other analytical techniques required. * Excellent, enthusiastic, clear communication skills, both written and verbal, with a diverse audience is critical to the success of this position. * Excellent customer interfacing and technical sales skills a must * Proven ability to interact with cross - functional and cross - cultural teams * Ability to travel both domestically as well as internationally and possession of a valid passport.
please submit your resume online at http://careers.fei.com/applyFEI2.asp?fei?fei1374-HBO?jjeske?
Thanks,
Jasmine Jeske Global Recruiting Manager FEI Company
==============================Original Headers============================== 17, 27 -- From Resume-at-fei.com Fri May 30 14:09:16 2008 17, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UJ9FNA025197 17, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 14:09:16 -0500 17, 27 -- X-WSS-ID: 0K1P3VH-01-ZYK-01 17, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 17, 27 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 27E1096FE7C 17, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 12:09:17 -0700 (PDT) 17, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 17, 27 -- Fri, 30 May 2008 12:09:14 -0700 17, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 27 -- Content-class: urn:content-classes:message 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="us-ascii" 17, 27 -- Subject: Job opportunity at FEI - Titan Research Scientist - Eugene, OR 17, 27 -- Date: Fri, 30 May 2008 12:09:13 -0700 17, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB81174CD89-at-hlexc03.w2k.feico.com} 17, 27 -- X-MS-Has-Attach: 17, 27 -- X-MS-TNEF-Correlator: 17, 27 -- Thread-Topic: Job opportunity at FEI - Titan Research Scientist - Eugene, OR 17, 27 -- Thread-Index: AcjCiKWCA7lOhFDHQIOdbhx+ZkwjNg== 17, 27 -- From: "Resume" {Resume-at-fei.com} 17, 27 -- To: {Microscopy-at-Microscopy.Com} 17, 27 -- X-OriginalArrivalTime: 30 May 2008 19:09:14.0894 (UTC) FILETIME=[A6BF12E0:01C8C288] 17, 27 -- Content-Transfer-Encoding: 8bit 17, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4UJ9FNA025197 ==============================End of - Headers==============================
Job Location: Midwest, USA The RE in the TEM applications team is responsible for providing technical expertise in the support of FEI's North American sales efforts. Primary responsibilities include the following:
* Becoming a Titan expert and transitioning that expertise to our customers. Develop new techniques and applications for FEI partners and customers. Technical product expert assisting with all phases of the sales cycle. * Cultivating positive customer relationships and acting as a high level customer interface for specific instrument/applications issues, acting as a technical expert, explaining complex technology details, giving in depth presentations, and assisting in technique development. * Assisting FEI Service Engineers on instrument sign offs and specific application techniques. * Providing continuous feedback to the product groups on system performance, features, and problems while collaborating with marketing/development groups on future product developments.
Position Requirements -
This customer focused position is ideal for an experienced technologist wanting to be involved with a dynamic team and exposed to a constant variety of customer application areas. The successful candidate will possess the following combination of education and experience:
* At a minimum, MS degree in science discipline. Higher degree is greatly preferred. * 5 or more years experience in a relevant commercial/research/production establishment with recent experience utilizing state of the art, TEM technology in sample characterization. * Knowledge of advanced technology such as correctors as well as, operational optimization experience of analytical equipment and various energy dispersive spectrometers nor other analytical techniques required. * Excellent, enthusiastic, clear communication skills, both written and verbal, with a diverse audience is critical to the success of this position. * Excellent customer interfacing and technical sales skills a must * Proven ability to interact with cross - functional and cross - cultural teams * Ability to travel both domestically as well as internationally and possession of a valid passport.
Apply at http://careers.fei.com/applyFEI2.asp?fei?fei1426-HBO?jjeske?
Thanks,
Jasmine Jeske Global Recruiting Manager FEI Company
==============================Original Headers============================== 16, 27 -- From Resume-at-fei.com Fri May 30 14:12:51 2008 16, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UJCpOA003352 16, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 14:12:51 -0500 16, 27 -- X-WSS-ID: 0K1P41G-01-03I-01 16, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 16, 27 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 2099896FF26 16, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 12:12:52 -0700 (PDT) 16, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 16, 27 -- Fri, 30 May 2008 12:12:50 -0700 16, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 27 -- Content-class: urn:content-classes:message 16, 27 -- MIME-Version: 1.0 16, 27 -- Content-Type: text/plain; 16, 27 -- charset="us-ascii" 16, 27 -- Subject: Job Opportunity at FEI Company: TEM Research Engineer - MT 16, 27 -- Date: Fri, 30 May 2008 12:12:50 -0700 16, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB81174CD8A-at-hlexc03.w2k.feico.com} 16, 27 -- X-MS-Has-Attach: 16, 27 -- X-MS-TNEF-Correlator: 16, 27 -- Thread-Topic: Job Opportunity at FEI Company: TEM Research Engineer - MT 16, 27 -- Thread-Index: AcjCiSaAhYhtIkGqRIGYahTEj+0Z0g== 16, 27 -- From: "Resume" {Resume-at-fei.com} 16, 27 -- To: {Microscopy-at-Microscopy.Com} 16, 27 -- X-OriginalArrivalTime: 30 May 2008 19:12:50.0295 (UTC) FILETIME=[2722A870:01C8C289] 16, 27 -- Content-Transfer-Encoding: 8bit 16, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4UJCpOA003352 ==============================End of - Headers==============================
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Thanks,
Joe Williamson Sr. Technical Recruiter FEI Company
==============================Original Headers============================== 6, 27 -- From Resume-at-fei.com Fri May 30 14:16:57 2008 6, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UJGu3G015893 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 14:16:57 -0500 6, 27 -- X-WSS-ID: 0K1P48A-01-0AD-01 6, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 6, 27 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 22954970014 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 30 May 2008 12:16:58 -0700 (PDT) 6, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 6, 27 -- Fri, 30 May 2008 12:16:56 -0700 6, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 27 -- Content-class: urn:content-classes:message 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="us-ascii" 6, 27 -- Subject: Job openings: FEI Company Field Service Engineer positions - SEM and TEM 6, 27 -- Date: Fri, 30 May 2008 12:16:56 -0700 6, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB81174CD8B-at-hlexc03.w2k.feico.com} 6, 27 -- X-MS-Has-Attach: 6, 27 -- X-MS-TNEF-Correlator: 6, 27 -- Thread-Topic: Job openings: FEI Company Field Service Engineer positions - SEM and TEM 6, 27 -- Thread-Index: AcjCibkeTvU8DjueSJ6TZ+n03ps5pQ== 6, 27 -- From: "Resume" {Resume-at-fei.com} 6, 27 -- To: {Microscopy-at-Microscopy.Com} 6, 27 -- X-OriginalArrivalTime: 30 May 2008 19:16:56.0491 (UTC) FILETIME=[B9E12FB0:01C8C289] 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4UJGu3G015893 ==============================End of - Headers==============================
I made a Faraday cup for a JEOL using the primary beam stop device so it was movable and routed the wires through a feedthrough on a metal plate that fit one of the unused rear viewing window ports. It worked well. Larry
bigelow-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } The problem with trying to put a Farday cup into the end of a TEM } specimen rod is that most specimen rods are too thin to accommodate a } good one. The backscattering of electrons is the basic problem here. } For a Faraday cup to be really effective it needs to be one or two } centimeters deep in order to trap all the incident electrons, } otherwise a good fraction of them are lost by being backscattered } away and you don't get an accurate reading of the beam current } anyway. Unfortunately, in order for the specimen rod in most the } TEMs to fit between the objective lens pole pieces where it can } intercept the electron beam it can only be one or two millimeters } thick, and this would not allow the accommodation of a truly } effective Faraday cup. } } An alternative, and probably less expensive and more effective } approach, is to design an independent Faraday cup to be installed } through a port in the viewing chamber. I designed one such device } for a Philips EM several years ago, and as far as I know it worked } out very satisfactorily. If anyone is interested I will be happy to } give them more details.
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 5, 28 -- From Larry.Ackerman-at-ucsf.edu Fri May 30 15:31:22 2008 5, 28 -- Received: from emfmcb02.ucsfmedicalcenter.org (emfmcb02.ucsfmedicalcenter.org [64.54.46.98]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UKVL2o007336 5, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2008 15:31:22 -0500 5, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 5, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 5, 28 -- Fri, 30 May 2008 13:28:00 -0700 5, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 5, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 5, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Fri, 30 May 2008 13:28:08 -0700 5, 28 -- Message-ID: {48406357.9080107-at-ucsf.edu} 5, 28 -- Date: Fri, 30 May 2008 13:28:07 -0700 5, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 5, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 5, 28 -- Organization: UCSF, NeuroAnatomy 5, 28 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 5, 28 -- MIME-Version: 1.0 5, 28 -- To: Microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] Faraday cup in a TEM 5, 28 -- References: {200805291931.m4TJV2YC021872-at-ns.microscopy.com} 5, 28 -- In-Reply-To: {200805291931.m4TJV2YC021872-at-ns.microscopy.com} 5, 28 -- X-OriginalArrivalTime: 30 May 2008 20:28:08.0253 (UTC) 5, 28 -- FILETIME=[AC0C56D0:01C8C293] 5, 28 -- X-WSS-ID: 645EBCDA29O14477459-01-01 5, 28 -- Content-Type: text/plain; 5, 28 -- charset=iso-8859-1; 5, 28 -- format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The area measured from the images is in some way connected to the volume of the mitochondria. The connection must be made correctly for the volume of the mitochondria to be known.
The field of stereology studies this process. A good paper that exactly describes the problem of relating the cross section area to volume is the 1987 paper by Gundersen in Journal of Microscopy. The introductory material in that paper discusses this problem.
There is a superior stereological protocol that is able to estimate the volume of the mitochondria. The problem with this protocol is that it produces a volume weighted volume. This differs from the number weighted volume. The protocol is the PSI or point sampled intercept. Unfortunately, few software packages implement the protocol and fewer implement the protocol correctly. The CAST and newCAST implementations are correct and may be the only correct implementations of the protocol. Depending on the amount of work being done, i.e. the number of mitochondria that are sampled, it is possible to do this protocol manually and correctly.
Other stereological protocols that cannot be used are the nucleator and the planar rotator although these methods appear to provide the answer. There are fundamental reasons that these methods cannot be applied to your work.
The PSI is described by Gundersen and Jensen in the Journal of Microscopy.
Quoting TindallR-at-missouri.edu:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks to everyone who responded my question on emphasizing mitochondria } for TEM image analysis. Lots of stuff to try now. I also found some } interesting ideas in Hayat's book on Positive Staining Techniques----one } involved an odd protocol using glutaraldehyde fixation followed by a } three-hour stain in 5% phosphotungstic acid in 6.25% Na2SO4, followed by } dehyration in acidic ethanol. I'm giving that a whirl now. Finally } there is an 1992 article on using acetonitrile as the dehydrating } solvent as a way of emphasizing mitochondria (Microscopy Research and } Technique, Vol 21: 39-50). } } Here are the replies, in all their glory: } ___________________________________________ } } A long time since I have done any enzyme cytochemistry but some of these } old techniques may help. } } Test for cytochrome oxidase maybe, or Glutamic oxaloacetic transaminase. } There are quite a range of mitochondria enzymes that may possibly be } useful } } Some of these tests were lead based, some cerium chloride. Others used } DAB. } } There is a book called Electron Microscopic Cytochemsitry and } Immunocytochemistry in BioMedicine by Ogawa and Barka and also one of } the Glauert series by Lewis and Knight (Cytochemical staining methods } for electron microscopy) that had methods. } } } Allan Mitchell } ------------------------------------------------------------------------ } -- } } Just a thought that is really off the wall - but what about doing it } with immunohistochemistry. I've done the immunoEM with DAB in } monolayers infected with chlamydia with pre-embedding and it worked } well. The basic concept is the same, just a different Ab for a natural } occuring enzyme. It may work on LR White embedded muscle tissue. But } then again, it may not. } } My biochemistry days are far behind, so I am not sure what particular } enzyme you would be best to stain for, but I do remember that there are } some very specific enzymes for mitochondria, and believe there were some } light microscopy tests. } } On the other hand, as far as hand tracing, what does the client have } students for? ;-) } } Paul Hazelton } ------------------------------------------------------------------------ } --- } } 1) You are not considering doing this with LM, are you?.. There are some } LM stains that bring up mitochondria nicely. The one I used, } 15 years } ago as a student, was Mallory's phosphotungstic hematoxylin (unless I am } confusing it with something else :)). Also at the LM level, you can use } antibody to COX - cytochrome oxidase. That's much more recent, COX is a } legit mitochondria marker, as my kid would say... } } 2) For EM - cacodylate buffer, good fresh OsO4, UA treatment before } embedding (2% in water 2 h or 1.5% in 70% ethanol overnight), and not } too long in any of the dehydration or infiltration steps - but I know } you know all this yourself... } } 3) For automatic tracing of mitochondria, did you, or your client, apply } any smoothing/filtering? I am not familiar with Metamorph but did spend } quite a bit of time trying different ways of helping software detect the } edges and extract features from my EM images. } Something between median filtering and non-linear anisotropic diffusion } often solves the issue. Basically, these filters allow for more } smoothing with less feature loss. Among free software, IMOD and ImageJ } both will do these filters. They will also propagate a strong edge, e.g. } from section to the next section. If you send me one of those pictures, } I'll be happy to take a look. } } 4) For didactic value, if there are indeed students involved, nothing } will beat the good old fashioned cutting out the mitochondria from a } print with scissors and weighing on a balance. } } Vlad Speransky } ---------------------------------------------------------------------- } } Do you have "Theory and Practiced of Histological Techniques" by } Bancroft and Stevens, 5th edition? On page 350 it discusses a technique } to visualize mitochondria with aniline-acid fuchsin, called "Altmann's } technique for mitochondria". } } Page 612 discusses NADH diaphorase to demonstrate mitochondria on } unfixed cryostat sections. Mostly used for muscle enzyme } histochemistry. } } Rhonda Allen } -------------------------------------------------------------------- } } It is really difficult to use MetaMorph to analyze things that are not } fluorescently labelled, or can otherwise be isolated by setting } intensity threshholds...that's how it was designed. Its meant for } analysis of data collected in widefield or confocal fluorescence. } My guess is that unless you unearth a method that will make the mitos } really stand out as almost black against the rest of the muscle, your } client is going to have to resort to tracing. } I've got a number of clients that are also looking at mitos in EM, } either isolated or in tissue. One group is primarily interested in } structure under varying conditions, but another is also interested in } relative numbers within skeletal muscle in a mouse model for a muscular } disease. If you come across anything that will work in MM....please } share! } } Lee Cohen-Gould } ------------------------------------------------------------------------ } } An acetone dehydration in conventional processing clears a lot of } cytoplasm from cells vs. ethanol. Mitochondria, cytoskeletin, ER, golgi } etc. all remain but the background is much lighter making the organelles } stand out more. I have not tried the other suggestions that you list. } } A word of caution that you may already know: } } Depending on where the sections are taken from the muscle there can be } several hundred% differences in mitochondrial volume/size. I am } currently studying muscle cross sections (very close to cross) rather } than longitudinal views as originally requested by the investigator } because of this. } } Interested in seeing others reply. } Pat Connelly } ---------------------------------------------------------------------- } isn't there a mitochondria-specific fluorescence dye (mito- tracker or } so) which you might use straight on the embedded tissue (whether in } paraffin or cryosections or even on plastic semi-thin sections??) ? } } - just for counting mito's, if appears to me that TEM is narrowing your } field of view - more than necessary. Light microsopy? } } - for counting the real area - huuups - then I would not trust the } sections because a (single ultrathin) section might not give you an area } which is really representative. And is it the area your client is } interested in, or rather the VOLUME of the mito (which really counts)? } and then, cryoprocessing (only) will give you a representative image of } well preserved mitochondria (demanding, I know). } } Reinhard Rachel } ------------------------------------------------------------------------ } } Thanks again to all! } } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } } } ==============================Original Headers============================== } 33, 24 -- From TindallR-at-missouri.edu Fri May 30 13:43:14 2008 } 33, 24 -- Received: from um-nsmtpout1.um.umsystem.edu } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } 33, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4UIhERH003420 } 33, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 13:43:14 -0500 } 33, 24 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 33, 24 -- Fri, 30 May 2008 13:43:14 -0500 } 33, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 33, 24 -- Content-class: urn:content-classes:message } 33, 24 -- MIME-Version: 1.0 } 33, 24 -- Content-Type: text/plain; } 33, 24 -- charset="us-ascii" } 33, 24 -- Subject: Mitochondria query summary---Thanks!! (Long) } 33, 24 -- Date: Fri, 30 May 2008 13:43:14 -0500 } 33, 24 -- Message-ID: } {91108EF9255B394CBF8B7E3789814A4103CD76B0-at-UM-XMAIL08.um.umsystem.edu} } 33, 24 -- X-MS-Has-Attach: } 33, 24 -- X-MS-TNEF-Correlator: } 33, 24 -- Thread-Topic: Mitochondria query summary---Thanks!! (Long) } 33, 24 -- Thread-Index: AcjChQRq9fcHzZBUTQ+12Q6of7dHew== } 33, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 33, 24 -- To: {microscopy-at-microscopy.com} } 33, 24 -- Cc: "Naples, Scott Patrick (MU-Student)" {spnd36-at-mizzou.edu} } 33, 24 -- X-OriginalArrivalTime: 30 May 2008 18:43:14.0401 (UTC) } FILETIME=[049EBD10:01C8C285] } 33, 24 -- Content-Transfer-Encoding: 8bit } 33, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m4UIhERH003420 } ==============================End of - Headers============================== }
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 9, 30 -- From rboehrin-at-vt.edu Fri May 30 16:45:57 2008 9, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4ULjviF026255 9, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2008 16:45:57 -0500 9, 30 -- Received: from freya.cc.vt.edu (IDENT:mirapoint-at-[10.1.1.15]) 9, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id m4ULjuTY010604; 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 9, 30 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 9, 30 -- by freya.cc.vt.edu (MOS 3.8.6-GA) 9, 30 -- with ESMTP id APQ53488; 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 (EDT) 9, 30 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain [127.0.0.1]) 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id m4ULju4d016914; 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 9, 30 -- Received: (from apache-at-localhost) 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id m4ULjtQm016913; 9, 30 -- Fri, 30 May 2008 17:45:55 -0400 9, 30 -- Received: from 66.109.249.142.static.dejazzd.com (66.109.249.142.static.dejazzd.com [66.109.249.142]) 9, 30 -- by webmail.vt.edu (IMP) with HTTP; Fri, 30 May 2008 17:45:55 -0400 9, 30 -- Message-ID: {1212183955.48407593c3892-at-webmail.vt.edu} 9, 30 -- Date: Fri, 30 May 2008 17:45:55 -0400 9, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} 9, 30 -- To: TindallR-at-missouri.edu, Microscopy-at-microscopy.com 9, 30 -- Subject: Re: [Microscopy] Mitochondria query summary---Thanks!! (Long) 9, 30 -- References: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} 9, 30 -- In-Reply-To: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} 9, 30 -- MIME-Version: 1.0 9, 30 -- Content-Type: text/plain; charset=ISO-8859-1 9, 30 -- Content-Transfer-Encoding: 8bit 9, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
I would second the suggestion from the last subscriber, that the very best way of obtaining mitochondrial volume from 2-D images is to use stereology.
You will have no need for complicated specimen staining, or trying to get a computer to "think" the way you want it to.
Contrary to perceived ideas about applying these methods, they are very easy and take up much less time than any contour tracing work. They also seem to be very efficient.
If you just perform a volume density estimation, it will give you the volume of mitochondria occupying the cells you estimated (volume density). This is fine if you only want this. However, if you are looking into comparing treatments to cells, or comparing different cells, you run into the problem of the cell volume.
The volume density only gives you a ratio of mitochondria to cell volume. So, a small cell population may give you the same volume density for mitochondria as a large cell population, even though there is maybe twice the actual volume of mitochondria present.
To get an actual value for the mitochondrial volume, you therefore need to be able to compare it with a reference space such as the mean cell volume of the population. There are many ways of obtaining mean cell volume (such as the nucleator), but that is for another posting.
Performing a stereological analysis can be as easy as overlaying acetate sheets over micrographs, but if a computer is essential, then the CAST system supplied by Olympus, or Stereo Investigator by MicroBrightField (no commercial interests etc) can supply you with the software and instruction needed.
I thought tracing or cutting went out of vogue many years ago so I was interested to read the comments in previous posts. I was waiting for a stereologist to join in and educate us all.
In addition to the paper cited in the previous post, there are two good reviews in APMIS by Gundersen et al. Look also for reviews by Terry Mayhew on this subject.
Regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
------ Forwarded Message } From: {rboehrin-at-vt.edu} } Reply-To: {rboehrin-at-vt.edu} } Date: Fri, 30 May 2008 16:50:10 -0500 } To: Paul Webster {PWebster-at-hei.org} } Subject: [Microscopy] Re: Mitochondria query summary---Thanks!! (Long) } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The area measured from the images is in some way connected to the volume of } the } mitochondria. The connection must be made correctly for the volume of the } mitochondria to be known. } } The field of stereology studies this process. A good paper that exactly } describes the problem of relating the cross section area to volume is the 1987 } paper by Gundersen in Journal of Microscopy. The introductory material in that } paper discusses this problem. } } There is a superior stereological protocol that is able to estimate the volume } of the mitochondria. The problem with this protocol is that it produces a } volume weighted volume. This differs from the number weighted volume. The } protocol is the PSI or point sampled intercept. Unfortunately, few software } packages implement the protocol and fewer implement the protocol correctly. } The } CAST and newCAST implementations are correct and may be the only correct } implementations of the protocol. Depending on the amount of work being done, } i.e. the number of mitochondria that are sampled, it is possible to do this } protocol manually and correctly. } } Other stereological protocols that cannot be used are the nucleator and the } planar rotator although these methods appear to provide the answer. There are } fundamental reasons that these methods cannot be applied to your work. } } The PSI is described by Gundersen and Jensen in the Journal of Microscopy. } } Quoting TindallR-at-missouri.edu: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Thanks to everyone who responded my question on emphasizing mitochondria } } for TEM image analysis. Lots of stuff to try now. I also found some } } interesting ideas in Hayat's book on Positive Staining Techniques----one } } involved an odd protocol using glutaraldehyde fixation followed by a } } three-hour stain in 5% phosphotungstic acid in 6.25% Na2SO4, followed by } } dehyration in acidic ethanol. I'm giving that a whirl now. Finally } } there is an 1992 article on using acetonitrile as the dehydrating } } solvent as a way of emphasizing mitochondria (Microscopy Research and } } Technique, Vol 21: 39-50). } } } } Here are the replies, in all their glory: } } ___________________________________________ } } } } A long time since I have done any enzyme cytochemistry but some of these } } old techniques may help. } } } } Test for cytochrome oxidase maybe, or Glutamic oxaloacetic transaminase. } } There are quite a range of mitochondria enzymes that may possibly be } } useful } } } } Some of these tests were lead based, some cerium chloride. Others used } } DAB. } } } } There is a book called Electron Microscopic Cytochemsitry and } } Immunocytochemistry in BioMedicine by Ogawa and Barka and also one of } } the Glauert series by Lewis and Knight (Cytochemical staining methods } } for electron microscopy) that had methods. } } } } } } Allan Mitchell } } ------------------------------------------------------------------------ } } -- } } } } Just a thought that is really off the wall - but what about doing it } } with immunohistochemistry. I've done the immunoEM with DAB in } } monolayers infected with chlamydia with pre-embedding and it worked } } well. The basic concept is the same, just a different Ab for a natural } } occuring enzyme. It may work on LR White embedded muscle tissue. But } } then again, it may not. } } } } My biochemistry days are far behind, so I am not sure what particular } } enzyme you would be best to stain for, but I do remember that there are } } some very specific enzymes for mitochondria, and believe there were some } } light microscopy tests. } } } } On the other hand, as far as hand tracing, what does the client have } } students for? ;-) } } } } Paul Hazelton } } ------------------------------------------------------------------------ } } --- } } } } 1) You are not considering doing this with LM, are you?.. There are some } } LM stains that bring up mitochondria nicely. The one I used, } 15 years } } ago as a student, was Mallory's phosphotungstic hematoxylin (unless I am } } confusing it with something else :)). Also at the LM level, you can use } } antibody to COX - cytochrome oxidase. That's much more recent, COX is a } } legit mitochondria marker, as my kid would say... } } } } 2) For EM - cacodylate buffer, good fresh OsO4, UA treatment before } } embedding (2% in water 2 h or 1.5% in 70% ethanol overnight), and not } } too long in any of the dehydration or infiltration steps - but I know } } you know all this yourself... } } } } 3) For automatic tracing of mitochondria, did you, or your client, apply } } any smoothing/filtering? I am not familiar with Metamorph but did spend } } quite a bit of time trying different ways of helping software detect the } } edges and extract features from my EM images. } } Something between median filtering and non-linear anisotropic diffusion } } often solves the issue. Basically, these filters allow for more } } smoothing with less feature loss. Among free software, IMOD and ImageJ } } both will do these filters. They will also propagate a strong edge, e.g. } } from section to the next section. If you send me one of those pictures, } } I'll be happy to take a look. } } } } 4) For didactic value, if there are indeed students involved, nothing } } will beat the good old fashioned cutting out the mitochondria from a } } print with scissors and weighing on a balance. } } } } Vlad Speransky } } ---------------------------------------------------------------------- } } } } Do you have "Theory and Practiced of Histological Techniques" by } } Bancroft and Stevens, 5th edition? On page 350 it discusses a technique } } to visualize mitochondria with aniline-acid fuchsin, called "Altmann's } } technique for mitochondria". } } } } Page 612 discusses NADH diaphorase to demonstrate mitochondria on } } unfixed cryostat sections. Mostly used for muscle enzyme } } histochemistry. } } } } Rhonda Allen } } -------------------------------------------------------------------- } } } } It is really difficult to use MetaMorph to analyze things that are not } } fluorescently labelled, or can otherwise be isolated by setting } } intensity threshholds...that's how it was designed. Its meant for } } analysis of data collected in widefield or confocal fluorescence. } } My guess is that unless you unearth a method that will make the mitos } } really stand out as almost black against the rest of the muscle, your } } client is going to have to resort to tracing. } } I've got a number of clients that are also looking at mitos in EM, } } either isolated or in tissue. One group is primarily interested in } } structure under varying conditions, but another is also interested in } } relative numbers within skeletal muscle in a mouse model for a muscular } } disease. If you come across anything that will work in MM....please } } share! } } } } Lee Cohen-Gould } } ------------------------------------------------------------------------ } } } } An acetone dehydration in conventional processing clears a lot of } } cytoplasm from cells vs. ethanol. Mitochondria, cytoskeletin, ER, golgi } } etc. all remain but the background is much lighter making the organelles } } stand out more. I have not tried the other suggestions that you list. } } } } A word of caution that you may already know: } } } } Depending on where the sections are taken from the muscle there can be } } several hundred% differences in mitochondrial volume/size. I am } } currently studying muscle cross sections (very close to cross) rather } } than longitudinal views as originally requested by the investigator } } because of this. } } } } Interested in seeing others reply. } } Pat Connelly } } ---------------------------------------------------------------------- } } isn't there a mitochondria-specific fluorescence dye (mito- tracker or } } so) which you might use straight on the embedded tissue (whether in } } paraffin or cryosections or even on plastic semi-thin sections??) ? } } } } - just for counting mito's, if appears to me that TEM is narrowing your } } field of view - more than necessary. Light microsopy? } } } } - for counting the real area - huuups - then I would not trust the } } sections because a (single ultrathin) section might not give you an area } } which is really representative. And is it the area your client is } } interested in, or rather the VOLUME of the mito (which really counts)? } } and then, cryoprocessing (only) will give you a representative image of } } well preserved mitochondria (demanding, I know). } } } } Reinhard Rachel } } ------------------------------------------------------------------------ } } } } Thanks again to all! } } } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } Week&NavType=Both&Type=TimePlan } } } } } } ==============================Original Headers============================== } } 33, 24 -- From TindallR-at-missouri.edu Fri May 30 13:43:14 2008 } } 33, 24 -- Received: from um-nsmtpout1.um.umsystem.edu } } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } 33, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } m4UIhERH003420 } } 33, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 13:43:14 -0500 } } 33, 24 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by } } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } 33, 24 -- Fri, 30 May 2008 13:43:14 -0500 } } 33, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 33, 24 -- Content-class: urn:content-classes:message } } 33, 24 -- MIME-Version: 1.0 } } 33, 24 -- Content-Type: text/plain; } } 33, 24 -- charset="us-ascii" } } 33, 24 -- Subject: Mitochondria query summary---Thanks!! (Long) } } 33, 24 -- Date: Fri, 30 May 2008 13:43:14 -0500 } } 33, 24 -- Message-ID: } } {91108EF9255B394CBF8B7E3789814A4103CD76B0-at-UM-XMAIL08.um.umsystem.edu} } } 33, 24 -- X-MS-Has-Attach: } } 33, 24 -- X-MS-TNEF-Correlator: } } 33, 24 -- Thread-Topic: Mitochondria query summary---Thanks!! (Long) } } 33, 24 -- Thread-Index: AcjChQRq9fcHzZBUTQ+12Q6of7dHew== } } 33, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } 33, 24 -- To: {microscopy-at-microscopy.com} } } 33, 24 -- Cc: "Naples, Scott Patrick (MU-Student)" {spnd36-at-mizzou.edu} } } 33, 24 -- X-OriginalArrivalTime: 30 May 2008 18:43:14.0401 (UTC) } } FILETIME=[049EBD10:01C8C285] } } 33, 24 -- Content-Transfer-Encoding: 8bit } } 33, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id m4UIhERH003420 } } ==============================End of - Headers============================== } } } } } Robert Boehringer } MS student } Virginia Tech } } ==============================Original Headers============================== } 9, 30 -- From rboehrin-at-vt.edu Fri May 30 16:45:57 2008 } 9, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) } 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4ULjviF026255 } 9, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2008 16:45:57 -0500 } 9, 30 -- Received: from freya.cc.vt.edu (IDENT:mirapoint-at-[10.1.1.15]) } 9, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id } m4ULjuTY010604; } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 } 9, 30 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) } 9, 30 -- by freya.cc.vt.edu (MOS 3.8.6-GA) } 9, 30 -- with ESMTP id APQ53488; } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 (EDT) } 9, 30 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain [127.0.0.1]) } 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id m4ULju4d016914; } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 } 9, 30 -- Received: (from apache-at-localhost) } 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id m4ULjtQm016913; } 9, 30 -- Fri, 30 May 2008 17:45:55 -0400 } 9, 30 -- Received: from 66.109.249.142.static.dejazzd.com } (66.109.249.142.static.dejazzd.com [66.109.249.142]) } 9, 30 -- by webmail.vt.edu (IMP) with HTTP; Fri, 30 May 2008 17:45:55 -0400 } 9, 30 -- Message-ID: {1212183955.48407593c3892-at-webmail.vt.edu} } 9, 30 -- Date: Fri, 30 May 2008 17:45:55 -0400 } 9, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} } 9, 30 -- To: TindallR-at-missouri.edu, Microscopy-at-microscopy.com } 9, 30 -- Subject: Re: [Microscopy] Mitochondria query summary---Thanks!! } (Long) } 9, 30 -- References: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} } 9, 30 -- In-Reply-To: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} } 9, 30 -- MIME-Version: 1.0 } 9, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } 9, 30 -- Content-Transfer-Encoding: 8bit } 9, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 } ==============================End of - Headers==============================
------ End of Forwarded Message
==============================Original Headers============================== 16, 19 -- From PWebster-at-hei.org Fri May 30 18:03:29 2008 16, 19 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4UN3TMt009665 16, 19 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 18:03:29 -0500 16, 19 -- Received: from 10.10.42.125 ([10.10.42.125]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 16, 19 -- Fri, 30 May 2008 23:03:28 +0000 16, 19 -- User-Agent: Microsoft-Entourage/12.10.0.080409 16, 19 -- Date: Fri, 30 May 2008 16:03:25 -0700 16, 19 -- Subject: FW: [Microscopy] Re: Mitochondria query summary---Thanks!! (Long) 16, 19 -- From: "Webster, Paul" {PWebster-at-hei.org} 16, 19 -- To: {microscopy-at-microscopy.com} 16, 19 -- Message-ID: {C465D5CD.19654%PWebster-at-hei.org} 16, 19 -- Thread-Topic: [Microscopy] Re: Mitochondria query summary---Thanks!! (Long) 16, 19 -- Thread-Index: AcjCqV1DnJC1nGdCSQmSV2akUPu2hA== 16, 19 -- In-Reply-To: {200805302150.m4ULoA5g032134-at-ns.microscopy.com} 16, 19 -- Mime-version: 1.0 16, 19 -- Content-type: text/plain; 16, 19 -- charset="US-ASCII" 16, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
If you are interested in a TEM analytical position at Intel in Hillsboro Oregon, please respond to Barbara.miner-at-intel.com All positions include working one weekend day.
Business Group Overview As the world's largest chip manufacturer, Intel strives to make every facet of semiconductor manufacturing state-of-the-art -- from semiconductor process development and manufacturing, through yield improvement to final test and optimization, and lastly packaging. Employees in the Technology and Manufacturing group are part of a worldwide network of manufacturing and assembly/test facilities. The analytical labs in Hillsboro support the development of next-generation microprocessors and are connected through the worldwide lab network to labs supporting manufacturing.
Qualifications You must have recently graduated -- within one year -- with a Ph.D. in Materials Science, Physics or Chemistry and have extensive experience using imaging and analytical TEM techniques. Additional qualifications include: - A strong background in thin film deposition and characterization - Good knowledge in semiconductor processing and associated materials - Excellent communication skills, both written and verbal - Capable of driving multiple projects independently and simultaneously - Creative and flexible to thrive under changing priorities - Ability to become a strong team contributor as well as an individual contributor - You must be eligible to work in the U.S.
Responsibilities In this position, you will be working in the materials lab that is chartered to support Intel's advanced technology development. Your responsibilities will include but not be limited to: - Accountable for TEM, STEM, EDX and EELS analysis of advanced IC devices - Closing collaborations with device and process development engineers to determine the relationship of materials properties to electrical performance - Collaborating with lab engineers specializing in a variety of complementary analytical techniques for example, SIMS, SEM, XPS - Reporting analytical results at various working forums - Training and mentoring technicians and junior engineers
==============================Original Headers============================== 9, 30 -- From barbara.miner-at-intel.com Fri May 30 19:17:52 2008 9, 30 -- Received: from mga14.intel.com (mga14.intel.com [143.182.124.37]) 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4V0HpYi024960 9, 30 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 19:17:51 -0500 9, 30 -- Received: from azsmga001.ch.intel.com ([10.2.17.19]) 9, 30 -- by azsmga102.ch.intel.com with ESMTP; 30 May 2008 17:17:51 -0700 9, 30 -- X-ExtLoop1: 1 9, 30 -- X-IronPort-AV: E=Sophos;i="4.27,569,1204531200"; 9, 30 -- d="scan'208";a="254978739" 9, 30 -- Received: from fmsmsx333.amr.corp.intel.com ([132.233.42.2]) 9, 30 -- by azsmga001.ch.intel.com with ESMTP; 30 May 2008 17:17:51 -0700 9, 30 -- Received: from fmsmsx883.amr.corp.intel.com ([10.19.19.17]) by fmsmsx333.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 9, 30 -- Fri, 30 May 2008 17:17:50 -0700 9, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 30 -- Content-class: urn:content-classes:message 9, 30 -- MIME-Version: 1.0 9, 30 -- Content-Type: text/plain; 9, 30 -- charset="iso-8859-1" 9, 30 -- Subject: Job Opening for PhD TEM analyst at Intel in Oregon 9, 30 -- Date: Fri, 30 May 2008 17:17:50 -0700 9, 30 -- Message-ID: {FD456C29E8799A4486BDBC54092619480FE56B-at-fmsmsx883.amr.corp.intel.com} 9, 30 -- X-MS-Has-Attach: 9, 30 -- X-MS-TNEF-Correlator: 9, 30 -- Thread-Topic: Job Opening for PhD TEM analyst at Intel in Oregon 9, 30 -- Thread-Index: AcjCs8Kti+ABd7AJTMa8jO+6DMsDwg== 9, 30 -- From: "Miner, Barbara" {barbara.miner-at-intel.com} 9, 30 -- To: {microscopy-at-microscopy.com} 9, 30 -- X-OriginalArrivalTime: 31 May 2008 00:17:50.0781 (UTC) FILETIME=[C31336D0:01C8C2B3] 9, 30 -- Content-Transfer-Encoding: 8bit 9, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m4V0HpYi024960 ==============================End of - Headers==============================
Our administration wishes to explore the possibility of hiring a full-time, electronics engineer to service various high technology, electronics instruments on campus: NMR's (3), Mass Spectrometers (2), electron microscopes (2 SEM, 2 TEM), atomic force microscopes (3). Most of the instruments to be serviced range in age from 25 to 2 yrs of age. The "hope" would be to consolidate individual service contracts on the multiple instruments.
Does anyone have experience with this type (or similar) arrangement? If so:
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I forgot to mention that the glass slides for optical microscopy often have to be sticky to collect things. You can use standard clear [e.g. Sellotape] sticky tape on the glass slides, either double sided tape or just bend back the two ends of the tape to stick it down to the glass. I vaguely remember a technique that involved acetone to dissolve the Sellotape plastic away and leave the sticky residue, or you can buy slides already sticky:
You can use Vaseline or similar on the slide, but I think sticky tape would work best. This is different to the black sticky tape discs used on SEM stubs that are conductive, but I expect you can collect the sample in a similar way.
You can collect dust in the air by sedimentation (e.g. spore traps/gravity slides), brushing deep dust onto the slide, or by pushing the sticky slide against the carpet when looking for dust mites - e.g. the bedroom carpet edge by the skirting board that the vacuum cleaner often misses [often more dust mites are found on the bedroom carpet than the bed]. Every house will have them, although I've never found them that quickly. Normally the dust mites are alive and you watch them slowly move about on the slide under the optical microscope (something you don't get under the SEM). They may hide under fibres, probably to avoid the light. Try using a desk lamp to illuminate the sample from above as well as standard transmission on the microscope. Things like insect wings, butterfly scales, insect eyes, pollen etc.. also look good under the optical microscope. You might be able to buy prepared slides of things like bed bugs, tape worms and fleas or get images out of books. Like all school demos, do try out your sampling ideas first.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: Smithcf-at-hotmail.com [mailto:Smithcf-at-hotmail.com] Sent: 28 May 2008 03:41 To: kjmorris-at-well.ox.ac.uk
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Smithcf-at-hotmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, May 27, 2008 at 15:44:03 ---------------------------------------------------------------------------
Email: Smithcf-at-hotmail.com Name: Chuck Smith
Organization: University of Connecticut
Education: 9-12th Grade High School
Location: Storrs, CT USA
Question: I'm teaching a week-long class on biodiversity this summer for middle school/high school students. I'd like one exercise to be a demonstration of biodiversity living on the human body, perhaps by having students collect samples from themselves (skin scrapings, using masking tape to lift samples, samples from carpets, etc.) and looking at these using EM. Our EM department has graciously offered their services to prepare the samples, but I'm in need of information on how and where to collect samples (eyelash mites, dust mites....). Any suggestions would be greatly appreciated.
OK, It's not a Faraday cup, but you can get a pretty reasonable number if you use the metering devices on the JEOL. You can use both the main screen and the spot screen. Both will give you the current measurements. By knowing the diameter of the one that you are using and exbanding the beam out to the diameter of the one that you are using, you can divide the current by the area and get a measured current density. As long as the beam is less than that diameter, you will get the measured current. You will see that it is constant as you change the diameter of the beam until part of the beam starts missing the plate being used. If you ever do use a Faraday cup once, you can measure the difference and that will be due to backscattered and secondary electrons lost from the phosphor screen. After that, you can always make the correction from the measured value on the screen. If all you want to do is set up reproducible beam current settings for experiments, it works very well for doing that.
-Scott
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I made a Faraday cup for a JEOL using the primary beam stop device so it } was movable and routed the wires through a feedthrough on a metal plate } that fit one of the unused rear viewing window ports. It worked well. } Larry } } bigelow-at-umich.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } The problem with trying to put a Farday cup into the end of a TEM } } specimen rod is that most specimen rods are too thin to accommodate a } } good one. The backscattering of electrons is the basic problem here. } } For a Faraday cup to be really effective it needs to be one or two } } centimeters deep in order to trap all the incident electrons, } } otherwise a good fraction of them are lost by being backscattered } } away and you don't get an accurate reading of the beam current } } anyway. Unfortunately, in order for the specimen rod in most the } } TEMs to fit between the objective lens pole pieces where it can } } intercept the electron beam it can only be one or two millimeters } } thick, and this would not allow the accommodation of a truly } } effective Faraday cup. } } } } An alternative, and probably less expensive and more effective } } approach, is to design an independent Faraday cup to be installed } } through a port in the viewing chamber. I designed one such device } } for a Philips EM several years ago, and as far as I know it worked } } out very satisfactorily. If anyone is interested I will be happy to } } give them more details. } } -- } Larry Ackerman, Associate Specialist } UCSF, Dept. of Anatomy, Rm S1347 } 513 Parnassus Ave., Box 0452 } San Francisco, CA 94143 } } larry.ackerman-at-ucsf.edu } } } ==============================Original } Headers============================== } 5, 28 -- From Larry.Ackerman-at-ucsf.edu Fri May 30 15:31:22 2008 } 5, 28 -- Received: from emfmcb02.ucsfmedicalcenter.org } (emfmcb02.ucsfmedicalcenter.org [64.54.46.98]) } 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4UKVL2o007336 } 5, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2008 15:31:22 -0500 } 5, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org } with } 5, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall } v6.1.0)); } 5, 28 -- Fri, 30 May 2008 13:28:00 -0700 } 5, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD } 5, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by } exvs06.net.ucsf.edu } 5, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Fri, 30 May 2008 13:28:08 } -0700 } 5, 28 -- Message-ID: {48406357.9080107-at-ucsf.edu} } 5, 28 -- Date: Fri, 30 May 2008 13:28:07 -0700 } 5, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} } 5, 28 -- Reply-to: larry.ackerman-at-ucsf.edu } 5, 28 -- Organization: UCSF, NeuroAnatomy } 5, 28 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) } 5, 28 -- MIME-Version: 1.0 } 5, 28 -- To: Microscopy-at-microscopy.com } 5, 28 -- Subject: Re: [Microscopy] Faraday cup in a TEM } 5, 28 -- References: {200805291931.m4TJV2YC021872-at-ns.microscopy.com} } 5, 28 -- In-Reply-To: {200805291931.m4TJV2YC021872-at-ns.microscopy.com} } 5, 28 -- X-OriginalArrivalTime: 30 May 2008 20:28:08.0253 (UTC) } 5, 28 -- FILETIME=[AC0C56D0:01C8C293] } 5, 28 -- X-WSS-ID: 645EBCDA29O14477459-01-01 } 5, 28 -- Content-Type: text/plain; } 5, 28 -- charset=iso-8859-1; } 5, 28 -- format=flowed } 5, 28 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== } }
-Scott
Scott Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Calejon San Clemente, CA 92673
1-800-728-2233 www.SouthBayTech.com
==============================Original Headers============================== 9, 23 -- From Walck-at-SouthBayTech.com Fri May 30 22:43:23 2008 9, 23 -- Received: from wbm7.pair.net (wbm7.pair.net [209.68.4.129]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m4V3hNtd004318 9, 23 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 22:43:23 -0500 9, 23 -- Received: by wbm7.pair.net (Postfix, from userid 65534) 9, 23 -- id 912631051B; Fri, 30 May 2008 23:43:21 -0400 (EDT) 9, 23 -- Received: from 24.127.148.8 ([24.127.148.8]) 9, 23 -- (SquirrelMail authenticated user walck-at-southbaytech.com) 9, 23 -- by webmail7.pair.com with HTTP; 9, 23 -- Fri, 30 May 2008 20:43:21 -0700 (PDT) 9, 23 -- Message-ID: {50188.24.127.148.8.1212205401.squirrel-at-webmail7.pair.com} 9, 23 -- In-Reply-To: {200805302040.m4UKe2cw011757-at-ns.microscopy.com} 9, 23 -- References: {200805302040.m4UKe2cw011757-at-ns.microscopy.com} 9, 23 -- Date: Fri, 30 May 2008 20:43:21 -0700 (PDT) 9, 23 -- Subject: Re: [Microscopy] Re: Faraday cup in a TEM 9, 23 -- From: "Scott Walck" {Walck-at-SouthBayTech.com} 9, 23 -- To: microscopy-at-microscopy.com 9, 23 -- User-Agent: SquirrelMail/1.4.5 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain;charset=iso-8859-1 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-Priority: 3 (Normal) 9, 23 -- Importance: Normal ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both topalemel-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: topalemel-at-yahoo.com Name: Emel Topal
Organization: Arizona State University Biodesign Institute
Title-Subject: [Filtered] immuno-TEM of NT-1 callus cells
Question: our research group is planning to do on-section immuno-labeling for TEM with NT-1 callus cells. We are in need of assistance with a protocol for processing the cells. If anyone has experience with these cells and can provide a procedure we would appreciate it. Thank you,
PSI does not generate a volume fraction. That can be easily done by point counting techniques. The PSI, the point sampled intercepts, is a volume estimation method.
Volume density is not a good method to use as are densities in general. A density is a ratio of quantities and the results can change when either quantity or both are changing. Absolute quantities are better to use and should always be determined. This is described well in the previous posting by Paul Webster.
The nucleator as I previously mentioned is not a good possibility for the volume estimation of mitochondria. The same technical issues extend to the planar rotator as well.
Tracing is not a viable option either. Tracing provides area. Areas cannot be compared either. The area of profiles is a measurement of the sectioning process and not a measurement of the original 3D structure which is volume. Cutting and weighing provides the same answers as tracing does. Another similar method is the use of planimeters. Still the result is area.
The connection between area and volume is described the G&J article in 1987. The result is unbiased only if the height of the object is known. This is a measurement taken perpendicular to the sectioning orientation. The section taken must also be a random section through the object being studied.
Volume estimation can be accomplished by exhaustive measurements of serial sections taken of the same object. These provide the profile areas at various positions through the object. The total area times the section thickness is a volume estimate. The difficulty in obtaining exhaustive serial sections of the same mitochondria is great. The amount of work is great.
The variance of the population being studied is an important issue. The range of sizes has to be considered. A solution is to find a means of quickly estimating the volume of mitochondria using single sections. That way a large number of mitochondria can be considered. The "Do more,less well" thinking of stereology is that it is better to address more individuals than it is to know any one individual well.
The nucleator and other local stereological methods fail due to the issue of selecting a reference point in a manner that is independent of the sectioning orientation. The PSI avoids this issue.
Quoting PWebster-at-hei.org:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } I would second the suggestion from the last subscriber, that the very best } way of obtaining mitochondrial volume from 2-D images is to use stereology. } } You will have no need for complicated specimen staining, or trying to get a } computer to "think" the way you want it to. } } Contrary to perceived ideas about applying these methods, they are very easy } and take up much less time than any contour tracing work. They also seem to } be very efficient. } } If you just perform a volume density estimation, it will give you the volume } of mitochondria occupying the cells you estimated (volume density). This is } fine if you only want this. However, if you are looking into comparing } treatments to cells, or comparing different cells, you run into the problem } of the cell volume. } } The volume density only gives you a ratio of mitochondria to cell volume. } So, a small cell population may give you the same volume density for } mitochondria as a large cell population, even though there is maybe twice } the actual volume of mitochondria present. } } To get an actual value for the mitochondrial volume, you therefore need to } be able to compare it with a reference space such as the mean cell volume of } the population. There are many ways of obtaining mean cell volume (such as } the nucleator), but that is for another posting. } } Performing a stereological analysis can be as easy as overlaying acetate } sheets over micrographs, but if a computer is essential, then the CAST } system supplied by Olympus, or Stereo Investigator by MicroBrightField (no } commercial interests etc) can supply you with the software and instruction } needed. } } I thought tracing or cutting went out of vogue many years ago so I was } interested to read the comments in previous posts. I was waiting for a } stereologist to join in and educate us all. } } In addition to the paper cited in the previous post, there are two good } reviews in APMIS by Gundersen et al. Look also for reviews by Terry Mayhew } on this subject. } } Regards, } } Paul. } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } ------ Forwarded Message } } From: {rboehrin-at-vt.edu} } } Reply-To: {rboehrin-at-vt.edu} } } Date: Fri, 30 May 2008 16:50:10 -0500 } } To: Paul Webster {PWebster-at-hei.org} } } Subject: [Microscopy] Re: Mitochondria query summary---Thanks!! (Long) } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } The area measured from the images is in some way connected to the volume of } } the } } mitochondria. The connection must be made correctly for the volume of the } } mitochondria to be known. } } } } The field of stereology studies this process. A good paper that exactly } } describes the problem of relating the cross section area to volume is the } 1987 } } paper by Gundersen in Journal of Microscopy. The introductory material in } that } } paper discusses this problem. } } } } There is a superior stereological protocol that is able to estimate the } volume } } of the mitochondria. The problem with this protocol is that it produces a } } volume weighted volume. This differs from the number weighted volume. The } } protocol is the PSI or point sampled intercept. Unfortunately, few software } } packages implement the protocol and fewer implement the protocol correctly. } } The } } CAST and newCAST implementations are correct and may be the only correct } } implementations of the protocol. Depending on the amount of work being } done, } } i.e. the number of mitochondria that are sampled, it is possible to do this } } protocol manually and correctly. } } } } Other stereological protocols that cannot be used are the nucleator and the } } planar rotator although these methods appear to provide the answer. There } are } } fundamental reasons that these methods cannot be applied to your work. } } } } The PSI is described by Gundersen and Jensen in the Journal of Microscopy. } } } } Quoting TindallR-at-missouri.edu: } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } } } } Thanks to everyone who responded my question on emphasizing mitochondria } } } for TEM image analysis. Lots of stuff to try now. I also found some } } } interesting ideas in Hayat's book on Positive Staining Techniques----one } } } involved an odd protocol using glutaraldehyde fixation followed by a } } } three-hour stain in 5% phosphotungstic acid in 6.25% Na2SO4, followed by } } } dehyration in acidic ethanol. I'm giving that a whirl now. Finally } } } there is an 1992 article on using acetonitrile as the dehydrating } } } solvent as a way of emphasizing mitochondria (Microscopy Research and } } } Technique, Vol 21: 39-50). } } } } } } Here are the replies, in all their glory: } } } ___________________________________________ } } } } } } A long time since I have done any enzyme cytochemistry but some of these } } } old techniques may help. } } } } } } Test for cytochrome oxidase maybe, or Glutamic oxaloacetic transaminase. } } } There are quite a range of mitochondria enzymes that may possibly be } } } useful } } } } } } Some of these tests were lead based, some cerium chloride. Others used } } } DAB. } } } } } } There is a book called Electron Microscopic Cytochemsitry and } } } Immunocytochemistry in BioMedicine by Ogawa and Barka and also one of } } } the Glauert series by Lewis and Knight (Cytochemical staining methods } } } for electron microscopy) that had methods. } } } } } } } } } Allan Mitchell } } } ------------------------------------------------------------------------ } } } -- } } } } } } Just a thought that is really off the wall - but what about doing it } } } with immunohistochemistry. I've done the immunoEM with DAB in } } } monolayers infected with chlamydia with pre-embedding and it worked } } } well. The basic concept is the same, just a different Ab for a natural } } } occuring enzyme. It may work on LR White embedded muscle tissue. But } } } then again, it may not. } } } } } } My biochemistry days are far behind, so I am not sure what particular } } } enzyme you would be best to stain for, but I do remember that there are } } } some very specific enzymes for mitochondria, and believe there were some } } } light microscopy tests. } } } } } } On the other hand, as far as hand tracing, what does the client have } } } students for? ;-) } } } } } } Paul Hazelton } } } ------------------------------------------------------------------------ } } } --- } } } } } } 1) You are not considering doing this with LM, are you?.. There are some } } } LM stains that bring up mitochondria nicely. The one I used, } 15 years } } } ago as a student, was Mallory's phosphotungstic hematoxylin (unless I am } } } confusing it with something else :)). Also at the LM level, you can use } } } antibody to COX - cytochrome oxidase. That's much more recent, COX is a } } } legit mitochondria marker, as my kid would say... } } } } } } 2) For EM - cacodylate buffer, good fresh OsO4, UA treatment before } } } embedding (2% in water 2 h or 1.5% in 70% ethanol overnight), and not } } } too long in any of the dehydration or infiltration steps - but I know } } } you know all this yourself... } } } } } } 3) For automatic tracing of mitochondria, did you, or your client, apply } } } any smoothing/filtering? I am not familiar with Metamorph but did spend } } } quite a bit of time trying different ways of helping software detect the } } } edges and extract features from my EM images. } } } Something between median filtering and non-linear anisotropic diffusion } } } often solves the issue. Basically, these filters allow for more } } } smoothing with less feature loss. Among free software, IMOD and ImageJ } } } both will do these filters. They will also propagate a strong edge, e.g. } } } from section to the next section. If you send me one of those pictures, } } } I'll be happy to take a look. } } } } } } 4) For didactic value, if there are indeed students involved, nothing } } } will beat the good old fashioned cutting out the mitochondria from a } } } print with scissors and weighing on a balance. } } } } } } Vlad Speransky } } } ---------------------------------------------------------------------- } } } } } } Do you have "Theory and Practiced of Histological Techniques" by } } } Bancroft and Stevens, 5th edition? On page 350 it discusses a technique } } } to visualize mitochondria with aniline-acid fuchsin, called "Altmann's } } } technique for mitochondria". } } } } } } Page 612 discusses NADH diaphorase to demonstrate mitochondria on } } } unfixed cryostat sections. Mostly used for muscle enzyme } } } histochemistry. } } } } } } Rhonda Allen } } } -------------------------------------------------------------------- } } } } } } It is really difficult to use MetaMorph to analyze things that are not } } } fluorescently labelled, or can otherwise be isolated by setting } } } intensity threshholds...that's how it was designed. Its meant for } } } analysis of data collected in widefield or confocal fluorescence. } } } My guess is that unless you unearth a method that will make the mitos } } } really stand out as almost black against the rest of the muscle, your } } } client is going to have to resort to tracing. } } } I've got a number of clients that are also looking at mitos in EM, } } } either isolated or in tissue. One group is primarily interested in } } } structure under varying conditions, but another is also interested in } } } relative numbers within skeletal muscle in a mouse model for a muscular } } } disease. If you come across anything that will work in MM....please } } } share! } } } } } } Lee Cohen-Gould } } } ------------------------------------------------------------------------ } } } } } } An acetone dehydration in conventional processing clears a lot of } } } cytoplasm from cells vs. ethanol. Mitochondria, cytoskeletin, ER, golgi } } } etc. all remain but the background is much lighter making the organelles } } } stand out more. I have not tried the other suggestions that you list. } } } } } } A word of caution that you may already know: } } } } } } Depending on where the sections are taken from the muscle there can be } } } several hundred% differences in mitochondrial volume/size. I am } } } currently studying muscle cross sections (very close to cross) rather } } } than longitudinal views as originally requested by the investigator } } } because of this. } } } } } } Interested in seeing others reply. } } } Pat Connelly } } } ---------------------------------------------------------------------- } } } isn't there a mitochondria-specific fluorescence dye (mito- tracker or } } } so) which you might use straight on the embedded tissue (whether in } } } paraffin or cryosections or even on plastic semi-thin sections??) ? } } } } } } - just for counting mito's, if appears to me that TEM is narrowing your } } } field of view - more than necessary. Light microsopy? } } } } } } - for counting the real area - huuups - then I would not trust the } } } sections because a (single ultrathin) section might not give you an area } } } which is really representative. And is it the area your client is } } } interested in, or rather the VOLUME of the mito (which really counts)? } } } and then, cryoprocessing (only) will give you a representative image of } } } well preserved mitochondria (demanding, I know). } } } } } } Reinhard Rachel } } } ------------------------------------------------------------------------ } } } } } } Thanks again to all! } } } } } } Randy } } } } } } Randy Tindall } } } Senior EM Specialist } } } Electron Microscopy Core Facility---We Do Small Well! } } } W125 Veterinary Medicine } } } University of Missouri } } } Columbia, MO 65211 } } } Tel: (573) 882-8304 } } } Fax: (573) 884-2227 } } } Email: tindallr-at-missouri.edu } } } Web: http://www.emc.missouri.edu } } } On-line calendar: } } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } } Week&NavType=Both&Type=TimePlan } } } } } } } } } ==============================Original } Headers============================== } } } 33, 24 -- From TindallR-at-missouri.edu Fri May 30 13:43:14 2008 } } } 33, 24 -- Received: from um-nsmtpout1.um.umsystem.edu } } } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } } 33, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } m4UIhERH003420 } } } 33, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 13:43:14 } -0500 } } } 33, 24 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by } } } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } } 33, 24 -- Fri, 30 May 2008 13:43:14 -0500 } } } 33, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } } 33, 24 -- Content-class: urn:content-classes:message } } } 33, 24 -- MIME-Version: 1.0 } } } 33, 24 -- Content-Type: text/plain; } } } 33, 24 -- charset="us-ascii" } } } 33, 24 -- Subject: Mitochondria query summary---Thanks!! (Long) } } } 33, 24 -- Date: Fri, 30 May 2008 13:43:14 -0500 } } } 33, 24 -- Message-ID: } } } {91108EF9255B394CBF8B7E3789814A4103CD76B0-at-UM-XMAIL08.um.umsystem.edu} } } } 33, 24 -- X-MS-Has-Attach: } } } 33, 24 -- X-MS-TNEF-Correlator: } } } 33, 24 -- Thread-Topic: Mitochondria query summary---Thanks!! (Long) } } } 33, 24 -- Thread-Index: AcjChQRq9fcHzZBUTQ+12Q6of7dHew== } } } 33, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } } 33, 24 -- To: {microscopy-at-microscopy.com} } } } 33, 24 -- Cc: "Naples, Scott Patrick (MU-Student)" {spnd36-at-mizzou.edu} } } } 33, 24 -- X-OriginalArrivalTime: 30 May 2008 18:43:14.0401 (UTC) } } } FILETIME=[049EBD10:01C8C285] } } } 33, 24 -- Content-Transfer-Encoding: 8bit } } } 33, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } } ns.microscopy.com id m4UIhERH003420 } } } ==============================End of - } Headers============================== } } } } } } } } } Robert Boehringer } } MS student } } Virginia Tech } } } } ==============================Original } Headers============================== } } 9, 30 -- From rboehrin-at-vt.edu Fri May 30 16:45:57 2008 } } 9, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu } [198.82.162.213]) } } 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } m4ULjviF026255 } } 9, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2008 16:45:57 -0500 } } 9, 30 -- Received: from freya.cc.vt.edu (IDENT:mirapoint-at-[10.1.1.15]) } } 9, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id } } m4ULjuTY010604; } } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 } } 9, 30 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) } } 9, 30 -- by freya.cc.vt.edu (MOS 3.8.6-GA) } } 9, 30 -- with ESMTP id APQ53488; } } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 (EDT) } } 9, 30 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain } [127.0.0.1]) } } 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id } m4ULju4d016914; } } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 } } 9, 30 -- Received: (from apache-at-localhost) } } 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id m4ULjtQm016913; } } 9, 30 -- Fri, 30 May 2008 17:45:55 -0400 } } 9, 30 -- Received: from 66.109.249.142.static.dejazzd.com } } (66.109.249.142.static.dejazzd.com [66.109.249.142]) } } 9, 30 -- by webmail.vt.edu (IMP) with HTTP; Fri, 30 May 2008 17:45:55 } -0400 } } 9, 30 -- Message-ID: {1212183955.48407593c3892-at-webmail.vt.edu} } } 9, 30 -- Date: Fri, 30 May 2008 17:45:55 -0400 } } 9, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} } } 9, 30 -- To: TindallR-at-missouri.edu, Microscopy-at-microscopy.com } } 9, 30 -- Subject: Re: [Microscopy] Mitochondria query summary---Thanks!! } } (Long) } } 9, 30 -- References: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} } } 9, 30 -- In-Reply-To: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} } } 9, 30 -- MIME-Version: 1.0 } } 9, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } } 9, 30 -- Content-Transfer-Encoding: 8bit } } 9, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 } } ==============================End of - } Headers============================== } } ------ End of Forwarded Message } } } ==============================Original Headers============================== } 16, 19 -- From PWebster-at-hei.org Fri May 30 18:03:29 2008 } 16, 19 -- Received: from hi0sml1.hei.org } (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) } 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m4UN3TMt009665 } 16, 19 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 18:03:29 -0500 } 16, 19 -- Received: from 10.10.42.125 ([10.10.42.125]) by hi0sml1.hei.org } ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; } 16, 19 -- Fri, 30 May 2008 23:03:28 +0000 } 16, 19 -- User-Agent: Microsoft-Entourage/12.10.0.080409 } 16, 19 -- Date: Fri, 30 May 2008 16:03:25 -0700 } 16, 19 -- Subject: FW: [Microscopy] Re: Mitochondria query summary---Thanks!! } (Long) } 16, 19 -- From: "Webster, Paul" {PWebster-at-hei.org} } 16, 19 -- To: {microscopy-at-microscopy.com} } 16, 19 -- Message-ID: {C465D5CD.19654%PWebster-at-hei.org} } 16, 19 -- Thread-Topic: [Microscopy] Re: Mitochondria query } summary---Thanks!! (Long) } 16, 19 -- Thread-Index: AcjCqV1DnJC1nGdCSQmSV2akUPu2hA== } 16, 19 -- In-Reply-To: {200805302150.m4ULoA5g032134-at-ns.microscopy.com} } 16, 19 -- Mime-version: 1.0 } 16, 19 -- Content-type: text/plain; } 16, 19 -- charset="US-ASCII" } 16, 19 -- Content-transfer-encoding: 7bit } ==============================End of - Headers============================== }
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 12, 30 -- From rboehrin-at-vt.edu Sun Jun 1 01:01:53 2008 12, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5161rxN032048 12, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Jun 2008 01:01:53 -0500 12, 30 -- Received: from zidane.cc.vt.edu (IDENT:mirapoint-at-evil-zidane.cc.vt.edu [10.1.1.13]) 12, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id m5161rhE013582 12, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Jun 2008 02:01:53 -0400 12, 30 -- Received: from imp-b07.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 12, 30 -- by zidane.cc.vt.edu (MOS 3.8.6-GA) 12, 30 -- with ESMTP id IUK54783; 12, 30 -- Sun, 1 Jun 2008 02:01:52 -0400 (EDT) 12, 30 -- Received: from imp-b07.cc.vt.edu (localhost.localdomain [127.0.0.1]) 12, 30 -- by imp-b07.cc.vt.edu (8.13.1/8.13.1) with ESMTP id m5161pkX029944 12, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 1 Jun 2008 02:01:51 -0400 12, 30 -- Received: (from apache-at-localhost) 12, 30 -- by imp-b07.cc.vt.edu (8.13.1/8.13.1/Submit) id m5161pFS029943 12, 30 -- for Microscopy-at-microscopy.com; Sun, 1 Jun 2008 02:01:51 -0400 12, 30 -- Received: from 66.109.249.142.static.dejazzd.com (66.109.249.142.static.dejazzd.com [66.109.249.142]) 12, 30 -- by webmail.vt.edu (IMP) with HTTP; Sun, 1 Jun 2008 02:01:51 -0400 12, 30 -- Message-ID: {1212300111.48423b4f83696-at-webmail.vt.edu} 12, 30 -- Date: Sun, 1 Jun 2008 02:01:51 -0400 12, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} 12, 30 -- To: Microscopy-at-microscopy.com 12, 30 -- Subject: Re: [Microscopy] FW: Re: Mitochondria query summary---Thanks!! (Long) 12, 30 -- References: {200805302310.m4UNA34v019484-at-ns.microscopy.com} 12, 30 -- In-Reply-To: {200805302310.m4UNA34v019484-at-ns.microscopy.com} 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; charset=ISO-8859-1 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
Recently, I have been trying to analyze an alloy which was supposed to have the following composition:
Fe30Co32Nb8B30 by atomic percent.
Using EDS system attached to our SEM, I can detect Boron levels of about 6 at.% in this alloy.
This alloy has a mosaic structure, grains on the order of few micron. We are using a Bruker brand XFlash EDS detector. Also, Nb has an M-line peak position at an energy slightly lower than Boron peak position.
Given all these negative effects about quantitative EDS analysis for this particular sample; is it possible to undershoot Boron amount by such a big amount. Or can I suspect that in this alloy (the surface available in EDS analysis) the real Boron amount is much lower than initially thought?
By the way, I have put a piece of pure Boron to check the detection limit of our EDS detector. In relatively short time (2-3 minutes), I have obtained a very strong Boron peak (~15 cps/eV). Background at low energies was less than ~1 cps/eV.
Thank you for your suggestions, Ayten Celik-Aktas, PhD
==============================Original Headers============================== 9, 27 -- From celikaktas-at-gmail.com Mon Jun 2 03:09:22 2008 9, 27 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.187]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5289MYs021381 9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 03:09:22 -0500 9, 27 -- Received: by fk-out-0910.google.com with SMTP id 18so895076fks.2 9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 02 Jun 2008 01:09:21 -0700 (PDT) 9, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 27 -- d=gmail.com; s=gamma; 9, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 9, 27 -- bh=G+xm0xeDkTf/F7QfsOBm5xiYWMamSp36s9CTOEyq6CI=; 9, 27 -- b=OZREQCzOTEwoccQV++BbW0usafzK4GYuSZhVOQh/zGcnKm76E9T/QxBd8Hx3EE7XyWB8zWcFmpBLHlaZXiir3tZnDJdHI7MNY6ZpUdolQ7cbgk8mV+RHp7o1uKDx8W2Q/tqToUcV1GGsuPzbjF9CX62O0DY21iK9LHFolIcqjtQ= 9, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 9, 27 -- d=gmail.com; s=gamma; 9, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 9, 27 -- b=QN8qjvGGTKBYbFZs0Rabgu6XFGnYT/qMswHeDrGG6Vy4wT83jDfyzckZY+O+XC3dTaBYt2bOLg7GbLUg3wLcKnuzx2RNpQU/8du2vLR+fKeyPqoNSxULS0/bNt1BU/5lOlqcm9TWgcHUpDfkULLlL1vaMW/QwQBsjavO2mVeD+U= 9, 27 -- Received: by 10.82.151.9 with SMTP id y9mr328047bud.8.1212394161151; 9, 27 -- Mon, 02 Jun 2008 01:09:21 -0700 (PDT) 9, 27 -- Received: by 10.82.125.7 with HTTP; Mon, 2 Jun 2008 01:09:21 -0700 (PDT) 9, 27 -- Message-ID: {1075c5c10806020109i4255efa1ya59bf2684ee8d091-at-mail.gmail.com} 9, 27 -- Date: Mon, 2 Jun 2008 11:09:21 +0300 9, 27 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} 9, 27 -- To: microscopy {Microscopy-at-microscopy.com} 9, 27 -- Subject: Detecting Boron with EDS system 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; charset=UTF-8 9, 27 -- Content-Transfer-Encoding: 7bit 9, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
As you observe, the Nb Mz line is at 169 eV versus your desired B Ka position at 184 eV.
I am skeptical that you will be able to do the analysis by EDS.
1. Is the software running an interference correction to back out the Boron? If so, you need to run some secondary standards to verify its accuracy (and the software should report intermediate negative values, rather than truncate [fudge] things to zero, which tells you nothing about how good an interference correction is doing). 2. What specific ZAF/phi rho Z and particularly what mass absorption coefficients are you running? There is a historic problem in EPMA for accurate determination of compositions where there is a wide range in Z of elements (here, 5 to 41, not as bad as say 14-77 or 78, though)
It is hard enough doing this by WDS...
John
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==============================Original Headers============================== 12, 26 -- From johnf-at-geology.wisc.edu Mon Jun 2 09:36:02 2008 12, 26 -- Received: from mail.geology.wisc.edu (mail.geology.wisc.edu [144.92.206.10]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m52Ea2c3018734 12, 26 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 09:36:02 -0500 12, 26 -- Received: from localhost (mail.geology.wisc.edu [127.0.0.1]) 12, 26 -- by localhost (Postfix) with ESMTP id C350C5C8114; 12, 26 -- Mon, 2 Jun 2008 09:36:00 -0500 (CDT) 12, 26 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu 12, 26 -- Received: from mail.geology.wisc.edu ([127.0.0.1]) 12, 26 -- by localhost (mail.geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 12, 26 -- with ESMTP id fkOdzta9O8de; Mon, 2 Jun 2008 09:35:54 -0500 (CDT) 12, 26 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 12, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 12, 26 -- (No client certificate requested) 12, 26 -- by mail.geology.wisc.edu (Postfix) with ESMTP id 3888F5C810E; 12, 26 -- Mon, 2 Jun 2008 09:35:54 -0500 (CDT) 12, 26 -- Mime-Version: 1.0 12, 26 -- Message-Id: {p06230903c469b3b29737-at-[144.92.206.57]} 12, 26 -- In-Reply-To: {200806020817.m528Hl32028290-at-ns.microscopy.com} 12, 26 -- References: {200806020817.m528Hl32028290-at-ns.microscopy.com} 12, 26 -- Date: Mon, 2 Jun 2008 09:35:52 -0500 12, 26 -- To: celikaktas-at-gmail.com 12, 26 -- From: John Fournelle {johnf-at-geology.wisc.edu} 12, 26 -- Subject: Re: [Microscopy] Detecting Boron with EDS system 12, 26 -- Cc: {microscopy-at-microscopy.com} 12, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
} From: rboehrin-at-vt.edu } Date: June 1, 2008 2:03:42 AM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] Re: FW: Re: Mitochondria query summary--- } Thanks!! (Long) } Reply-To: rboehrin-at-vt.edu }
} ... } Cutting and weighing provides the same answers as tracing does. } Another similar } method is the use of planimeters. Still the result is area. }
I only meant that as a joke, kind of attitude adjuster for students. It is of course much more time consuming, and harder to do accurately, than tracing.
Continuing in the lighter mood, I can see Randy's client getting all excited about doing it right with stereology...
Vlad _______________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 7, 23 -- From vladislav_speransky-at-nih.gov Mon Jun 2 12:03:12 2008 7, 23 -- Received: from nihrelayxway5.hub.nih.gov (nihrelayxway5.hub.nih.gov [128.231.90.99]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m52H3B9v005642 7, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 12:03:11 -0500 7, 23 -- X-IronPortListener: NIH_Relay 7, 23 -- X-SBRS: None 7, 23 -- X-IronPort-AV: E=Sophos;i="4.27,578,1204520400"; 7, 23 -- d="scan'208";a="179349141" 7, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 7, 23 -- by nihrelayxway5.hub.nih.gov with ESMTP; 02 Jun 2008 13:03:11 -0400 7, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 7, 23 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m52H3BgO17980531 7, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 13:03:11 -0400 (EDT) 7, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 7, 23 -- References: {200806010603.m5163gfv001019-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 23 -- Message-Id: {A40BB01B-B92F-419C-B2F5-C92DD2F22211-at-nih.gov} 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 7, 23 -- Subject: Fwd: [Microscopy] Re: FW: Re: Mitochondria query summary---Thanks!! (Long) 7, 23 -- Date: Mon, 2 Jun 2008 13:02:54 -0400 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
} The sectionning process is known, controllable and reproducable, how can it } change the measured area?
This is an excellent question. A number of great articles were written in the 1950s by Hans Elias. He had just published an important article in which he showed that for 99 years the structure of the mammalian liver was incorrectly stated in all textbooks. This was a direct result of an apparently universal misunderstanding of what is seen under the microscope. How would it be possible to get the numbers right if the qualitative statements of what is observed is not correct?
It is important to go over the repeated comment that "by randomly sectioning".
No one really knows whether or not a section randomly hits cells unless the sectioning was done in a random manner. It is often stated or assumed that something is random. There is a nice introduction in PAP Moran's book in which he solves a simple problem and uses 3 definitions of random. He gets 3 distinct answers: 1/4, 1/3, and 1/2. He asks, "What answer is the correct answer?" He immediately gives the answer, "All 3 are right."
To claim something is random is not meaningful without a definition of random. To claim that sections intersect mitochondria in some sort of random way is an untested claim.
Stereology provides definitions of random and makes use of those definitions to devise methods that provide the answer being sought. Stereology makes it possible to know if the answer is in some sense correct. Stereology avoids assumptions of randomness.
Here is a simple problem. Suppose that I had some tissue. Suppose that the mitochondria were perfect spheres. Suppose that the locations of the mitochondria are random in the sense that the positions of the mitochondria are independent of their sizes, the positions of the cells, and each other.
Now suppose that the tissue was sectioned and the diameters of the spherical mitochondria were measured as were the areas of the profiles of the mitochondria seen in the sections. Question 1: Is the average diameter of the measured diameters the average diameter of a mitochondria? Question 2: Is the average of the measured mitochondria areas related in any meaningful way to the volumes of the mitochondria?
I am only proposing this question with spheres because this is a simplification of the problem that has already been proposed.
You may suppose that the sampling is exhaustive, systematic, random, systematically random, or whatever you choose.
As many liver researchers learned, it is simple to be confused about is seen through the microscope.
PS Hans Elias also reported on a mistaken notion of mitochondria shape.
Quoting Stephane Nizet {nizets2-at-yahoo.com} :
} Hi! } } I wanted to react to the following remark: } } "Tracing is not a viable option either. Tracing provides area. Areas cannot } be } compared either. The area of profiles is a measurement of the sectioning } process and not a measurement of the original 3D structure which is volume. } Cutting and weighing provides the same answers as tracing does. Another } similar } method is the use of planimeters. Still the result is area." } } I don't really get the point. What does mean "the area of profiles is a } measurement of the sectioning process"? } The sectionning process is known, controllable and reproducable, how can it } change the measured area? } } One can estimate the volume of an object by randomly sectionning it multiple } times. That is exactly what happens whhen you cut cells!! } I second the opinion of Paul Webster. Although I am not a specialist in } stereology I heard several seminars on the subject (I won't cite names but } the people who gave them are not really tourists in the field). In one single } section there are a lot of mitochondria randomly sectioned! The most } important parameter here is to randomly select the area taken in picture and } to work systematically. } } Best regards, } } Stephane } } } } } } ----- Original Message ---- } From: "rboehrin-at-vt.edu" {rboehrin-at-vt.edu} } To: nizets2-at-yahoo.com } Sent: Sunday, June 1, 2008 8:09:55 AM } Subject: [Microscopy] Re: FW: Re: Mitochondria query summary---Thanks!! } (Long) } } } } Tracing is not a viable option either. Tracing provides area. Areas cannot be } compared either. The area of profiles is a measurement of the sectioning } process and not a measurement of the original 3D structure which is volume. } Cutting and weighing provides the same answers as tracing does. Another } similar } method is the use of planimeters. Still the result is area. } } Quoting PWebster-at-hei.org: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hello, } } } } I would second the suggestion from the last subscriber, that the very best } } way of obtaining mitochondrial volume from 2-D images is to use stereology. } } } } You will have no need for complicated specimen staining, or trying to get a } } computer to "think" the way you want it to. } } } } Contrary to perceived ideas about applying these methods, they are very } easy } } and take up much less time than any contour tracing work. They also seem to } } be very efficient. } } } } If you just perform a volume density estimation, it will give you the } volume } } of mitochondria occupying the cells you estimated (volume density). This is } } fine if you only want this. However, if you are looking into comparing } } treatments to cells, or comparing different cells, you run into the problem } } of the cell volume. } } } } The volume density only gives you a ratio of mitochondria to cell volume. } } So, a small cell population may give you the same volume density for } } mitochondria as a large cell population, even though there is maybe twice } } the actual volume of mitochondria present. } } } } To get an actual value for the mitochondrial volume, you therefore need to } } be able to compare it with a reference space such as the mean cell volume } of } } the population. There are many ways of obtaining mean cell volume (such as } } the nucleator), but that is for another posting. } } } } Performing a stereological analysis can be as easy as overlaying acetate } } sheets over micrographs, but if a computer is essential, then the CAST } } system supplied by Olympus, or Stereo Investigator by MicroBrightField (no } } commercial interests etc) can supply you with the software and instruction } } needed. } } } } I thought tracing or cutting went out of vogue many years ago so I was } } interested to read the comments in previous posts. I was waiting for a } } stereologist to join in and educate us all. } } } } In addition to the paper cited in the previous post, there are two good } } reviews in APMIS by Gundersen et al. Look also for reviews by Terry Mayhew } } on this subject. } } } } Regards, } } } } Paul. } } } } Paul Webster, Ph.D } } House Ear Institute } } 2100 West Third Street } } Los Angeles, CA 90057 } } (213) 273 8026 } } pwebster-at-hei.org } } } } ------ Forwarded Message } } } From: {rboehrin-at-vt.edu} } } } Reply-To: {rboehrin-at-vt.edu} } } } Date: Fri, 30 May 2008 16:50:10 -0500 } } } To: Paul Webster {PWebster-at-hei.org} } } } Subject: [Microscopy] Re: Mitochondria query summary---Thanks!! (Long) } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } The area measured from the images is in some way connected to the volume } of } } } the } } } mitochondria. The connection must be made correctly for the volume of the } } } mitochondria to be known. } } } } } } The field of stereology studies this process. A good paper that exactly } } } describes the problem of relating the cross section area to volume is the } } 1987 } } } paper by Gundersen in Journal of Microscopy. The introductory material in } } that } } } paper discusses this problem. } } } } } } There is a superior stereological protocol that is able to estimate the } } volume } } } of the mitochondria. The problem with this protocol is that it produces a } } } volume weighted volume. This differs from the number weighted volume. The } } } protocol is the PSI or point sampled intercept. Unfortunately, few } software } } } packages implement the protocol and fewer implement the protocol } correctly. } } } The } } } CAST and newCAST implementations are correct and may be the only correct } } } implementations of the protocol. Depending on the amount of work being } } done, } } } i.e. the number of mitochondria that are sampled, it is possible to do } this } } } protocol manually and correctly. } } } } } } Other stereological protocols that cannot be used are the nucleator and } the } } } planar rotator although these methods appear to provide the answer. There } } are } } } fundamental reasons that these methods cannot be applied to your work. } } } } } } The PSI is described by Gundersen and Jensen in the Journal of } Microscopy. } } } } } } Quoting TindallR-at-missouri.edu: } } } } } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } ---------------------------------------------------------------------------- } } } } } } } } Thanks to everyone who responded my question on emphasizing mitochondria } } } } for TEM image analysis. Lots of stuff to try now. I also found some } } } } interesting ideas in Hayat's book on Positive Staining Techniques----one } } } } involved an odd protocol using glutaraldehyde fixation followed by a } } } } three-hour stain in 5% phosphotungstic acid in 6.25% Na2SO4, followed by } } } } dehyration in acidic ethanol. I'm giving that a whirl now. Finally } } } } there is an 1992 article on using acetonitrile as the dehydrating } } } } solvent as a way of emphasizing mitochondria (Microscopy Research and } } } } Technique, Vol 21: 39-50). } } } } } } } } Here are the replies, in all their glory: } } } } ___________________________________________ } } } } } } } } A long time since I have done any enzyme cytochemistry but some of these } } } } old techniques may help. } } } } } } } } Test for cytochrome oxidase maybe, or Glutamic oxaloacetic transaminase. } } } } There are quite a range of mitochondria enzymes that may possibly be } } } } useful } } } } } } } } Some of these tests were lead based, some cerium chloride. Others used } } } } DAB. } } } } } } } } There is a book called Electron Microscopic Cytochemsitry and } } } } Immunocytochemistry in BioMedicine by Ogawa and Barka and also one of } } } } the Glauert series by Lewis and Knight (Cytochemical staining methods } } } } for electron microscopy) that had methods. } } } } } } } } } } } } Allan Mitchell } } } } ------------------------------------------------------------------------ } } } } -- } } } } } } } } Just a thought that is really off the wall - but what about doing it } } } } with immunohistochemistry. I've done the immunoEM with DAB in } } } } monolayers infected with chlamydia with pre-embedding and it worked } } } } well. The basic concept is the same, just a different Ab for a natural } } } } occuring enzyme. It may work on LR White embedded muscle tissue. But } } } } then again, it may not. } } } } } } } } My biochemistry days are far behind, so I am not sure what particular } } } } enzyme you would be best to stain for, but I do remember that there are } } } } some very specific enzymes for mitochondria, and believe there were some } } } } light microscopy tests. } } } } } } } } On the other hand, as far as hand tracing, what does the client have } } } } students for? ;-) } } } } } } } } Paul Hazelton } } } } ------------------------------------------------------------------------ } } } } --- } } } } } } } } 1) You are not considering doing this with LM, are you?.. There are some } } } } LM stains that bring up mitochondria nicely. The one I used, } 15 years } } } } ago as a student, was Mallory's phosphotungstic hematoxylin (unless I am } } } } confusing it with something else :)). Also at the LM level, you can use } } } } antibody to COX - cytochrome oxidase. That's much more recent, COX is a } } } } legit mitochondria marker, as my kid would say... } } } } } } } } 2) For EM - cacodylate buffer, good fresh OsO4, UA treatment before } } } } embedding (2% in water 2 h or 1.5% in 70% ethanol overnight), and not } } } } too long in any of the dehydration or infiltration steps - but I know } } } } you know all this yourself... } } } } } } } } 3) For automatic tracing of mitochondria, did you, or your client, apply } } } } any smoothing/filtering? I am not familiar with Metamorph but did spend } } } } quite a bit of time trying different ways of helping software detect the } } } } edges and extract features from my EM images. } } } } Something between median filtering and non-linear anisotropic diffusion } } } } often solves the issue. Basically, these filters allow for more } } } } smoothing with less feature loss. Among free software, IMOD and ImageJ } } } } both will do these filters. They will also propagate a strong edge, e.g. } } } } from section to the next section. If you send me one of those pictures, } } } } I'll be happy to take a look. } } } } } } } } 4) For didactic value, if there are indeed students involved, nothing } } } } will beat the good old fashioned cutting out the mitochondria from a } } } } print with scissors and weighing on a balance. } } } } } } } } Vlad Speransky } } } } ---------------------------------------------------------------------- } } } } } } } } Do you have "Theory and Practiced of Histological Techniques" by } } } } Bancroft and Stevens, 5th edition? On page 350 it discusses a technique } } } } to visualize mitochondria with aniline-acid fuchsin, called "Altmann's } } } } technique for mitochondria". } } } } } } } } Page 612 discusses NADH diaphorase to demonstrate mitochondria on } } } } unfixed cryostat sections. Mostly used for muscle enzyme } } } } histochemistry. } } } } } } } } Rhonda Allen } } } } -------------------------------------------------------------------- } } } } } } } } It is really difficult to use MetaMorph to analyze things that are not } } } } fluorescently labelled, or can otherwise be isolated by setting } } } } intensity threshholds...that's how it was designed. Its meant for } } } } analysis of data collected in widefield or confocal fluorescence. } } } } My guess is that unless you unearth a method that will make the mitos } } } } really stand out as almost black against the rest of the muscle, your } } } } client is going to have to resort to tracing. } } } } I've got a number of clients that are also looking at mitos in EM, } } } } either isolated or in tissue. One group is primarily interested in } } } } structure under varying conditions, but another is also interested in } } } } relative numbers within skeletal muscle in a mouse model for a muscular } } } } disease. If you come across anything that will work in MM....please } } } } share! } } } } } } } } Lee Cohen-Gould } } } } ------------------------------------------------------------------------ } } } } } } } } An acetone dehydration in conventional processing clears a lot of } } } } cytoplasm from cells vs. ethanol. Mitochondria, cytoskeletin, ER, golgi } } } } etc. all remain but the background is much lighter making the organelles } } } } stand out more. I have not tried the other suggestions that you list. } } } } } } } } A word of caution that you may already know: } } } } } } } } Depending on where the sections are taken from the muscle there can be } } } } several hundred% differences in mitochondrial volume/size. I am } } } } currently studying muscle cross sections (very close to cross) rather } } } } than longitudinal views as originally requested by the investigator } } } } because of this. } } } } } } } } Interested in seeing others reply. } } } } Pat Connelly } } } } ---------------------------------------------------------------------- } } } } isn't there a mitochondria-specific fluorescence dye (mito- tracker or } } } } so) which you might use straight on the embedded tissue (whether in } } } } paraffin or cryosections or even on plastic semi-thin sections??) ? } } } } } } } } - just for counting mito's, if appears to me that TEM is narrowing your } } } } field of view - more than necessary. Light microsopy? } } } } } } } } - for counting the real area - huuups - then I would not trust the } } } } sections because a (single ultrathin) section might not give you an area } } } } which is really representative. And is it the area your client is } } } } interested in, or rather the VOLUME of the mito (which really counts)? } } } } and then, cryoprocessing (only) will give you a representative image of } } } } well preserved mitochondria (demanding, I know). } } } } } } } } Reinhard Rachel } } } } ------------------------------------------------------------------------ } } } } } } } } Thanks again to all! } } } } } } } } Randy } } } } } } } } Randy Tindall } } } } Senior EM Specialist } } } } Electron Microscopy Core Facility---We Do Small Well! } } } } W125 Veterinary Medicine } } } } University of Missouri } } } } Columbia, MO 65211 } } } } Tel: (573) 882-8304 } } } } Fax: (573) 884-2227 } } } } Email: tindallr-at-missouri.edu } } } } Web: http://www.emc.missouri.edu } } } } On-line calendar: } } } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } } } } Week&NavType=Both&Type=TimePlan } } } } } } } } } } } } ==============================Original } } Headers============================== } } } } 33, 24 -- From TindallR-at-missouri.edu Fri May 30 13:43:14 2008 } } } } 33, 24 -- Received: from um-nsmtpout1.um.umsystem.edu } } } } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } } } } 33, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } } m4UIhERH003420 } } } } 33, 24 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 13:43:14 } } -0500 } } } } 33, 24 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) } by } } } } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } } } } 33, 24 -- Fri, 30 May 2008 13:43:14 -0500 } } } } 33, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } } } 33, 24 -- Content-class: urn:content-classes:message } } } } 33, 24 -- MIME-Version: 1.0 } } } } 33, 24 -- Content-Type: text/plain; } } } } 33, 24 -- charset="us-ascii" } } } } 33, 24 -- Subject: Mitochondria query summary---Thanks!! (Long) } } } } 33, 24 -- Date: Fri, 30 May 2008 13:43:14 -0500 } } } } 33, 24 -- Message-ID: } } } } {91108EF9255B394CBF8B7E3789814A4103CD76B0-at-UM-XMAIL08.um.umsystem.edu} } } } } 33, 24 -- X-MS-Has-Attach: } } } } 33, 24 -- X-MS-TNEF-Correlator: } } } } 33, 24 -- Thread-Topic: Mitochondria query summary---Thanks!! (Long) } } } } 33, 24 -- Thread-Index: AcjChQRq9fcHzZBUTQ+12Q6of7dHew== } } } } 33, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } } } } 33, 24 -- To: {microscopy-at-microscopy.com} } } } } 33, 24 -- Cc: "Naples, Scott Patrick (MU-Student)" {spnd36-at-mizzou.edu} } } } } 33, 24 -- X-OriginalArrivalTime: 30 May 2008 18:43:14.0401 (UTC) } } } } FILETIME=[049EBD10:01C8C285] } } } } 33, 24 -- Content-Transfer-Encoding: 8bit } } } } 33, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } } } ns.microscopy.com id m4UIhERH003420 } } } } ==============================End of - } } Headers============================== } } } } } } } } } } } } } Robert Boehringer } } } MS student } } } Virginia Tech } } } } } } ==============================Original } } Headers============================== } } } 9, 30 -- From rboehrin-at-vt.edu Fri May 30 16:45:57 2008 } } } 9, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu } } [198.82.162.213]) } } } 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } m4ULjviF026255 } } } 9, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 30 May 2008 16:45:57 } -0500 } } } 9, 30 -- Received: from freya.cc.vt.edu (IDENT:mirapoint-at-[10.1.1.15]) } } } 9, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id } } } m4ULjuTY010604; } } } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 } } } 9, 30 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) } } } 9, 30 -- by freya.cc.vt.edu (MOS 3.8.6-GA) } } } 9, 30 -- with ESMTP id APQ53488; } } } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 (EDT) } } } 9, 30 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain } } [127.0.0.1]) } } } 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id } } m4ULju4d016914; } } } 9, 30 -- Fri, 30 May 2008 17:45:56 -0400 } } } 9, 30 -- Received: (from apache-at-localhost) } } } 9, 30 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id m4ULjtQm016913; } } } 9, 30 -- Fri, 30 May 2008 17:45:55 -0400 } } } 9, 30 -- Received: from 66.109.249.142.static.dejazzd.com } } } (66.109.249.142.static.dejazzd.com [66.109.249.142]) } } } 9, 30 -- by webmail.vt.edu (IMP) with HTTP; Fri, 30 May 2008 17:45:55 } } -0400 } } } 9, 30 -- Message-ID: {1212183955.48407593c3892-at-webmail.vt.edu} } } } 9, 30 -- Date: Fri, 30 May 2008 17:45:55 -0400 } } } 9, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} } } } 9, 30 -- To: TindallR-at-missouri.edu, Microscopy-at-microscopy.com } } } 9, 30 -- Subject: Re: [Microscopy] Mitochondria query summary---Thanks!! } } } (Long) } } } 9, 30 -- References: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} } } } 9, 30 -- In-Reply-To: {200805301851.m4UIpmhK013472-at-ns.microscopy.com} } } } 9, 30 -- MIME-Version: 1.0 } } } 9, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } } } 9, 30 -- Content-Transfer-Encoding: 8bit } } } 9, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 } } } ==============================End of - } } Headers============================== } } } } ------ End of Forwarded Message } } } } } } ==============================Original } Headers============================== } } 16, 19 -- From PWebster-at-hei.org Fri May 30 18:03:29 2008 } } 16, 19 -- Received: from hi0sml1.hei.org } } (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) } } 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } m4UN3TMt009665 } } 16, 19 -- for {microscopy-at-microscopy.com} ; Fri, 30 May 2008 18:03:29 } -0500 } } 16, 19 -- Received: from 10.10.42.125 ([10.10.42.125]) by hi0sml1.hei.org } } ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; } } 16, 19 -- Fri, 30 May 2008 23:03:28 +0000 } } 16, 19 -- User-Agent: Microsoft-Entourage/12.10.0.080409 } } 16, 19 -- Date: Fri, 30 May 2008 16:03:25 -0700 } } 16, 19 -- Subject: FW: [Microscopy] Re: Mitochondria query } summary---Thanks!! } } (Long) } } 16, 19 -- From: "Webster, Paul" {PWebster-at-hei.org} } } 16, 19 -- To: {microscopy-at-microscopy.com} } } 16, 19 -- Message-ID: {C465D5CD.19654%PWebster-at-hei.org} } } 16, 19 -- Thread-Topic: [Microscopy] Re: Mitochondria query } } summary---Thanks!! 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(Long) } 12, 30 -- References: {200805302310.m4UNA34v019484-at-ns.microscopy.com} } 12, 30 -- In-Reply-To: {200805302310.m4UNA34v019484-at-ns.microscopy.com} } 12, 30 -- MIME-Version: 1.0 } 12, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } 12, 30 -- Content-Transfer-Encoding: 8bit } 12, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 } ==============================End of - Headers============================== } } } } }
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 18, 30 -- From rboehrin-at-vt.edu Mon Jun 2 15:19:47 2008 18, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 18, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m52KJkaO028953 18, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 15:19:46 -0500 18, 30 -- Received: from steiner.cc.vt.edu (IDENT:mirapoint-at-evil-steiner.cc.vt.edu [10.1.1.14]) 18, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id m52KJkOP013911 18, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 16:19:46 -0400 18, 30 -- Received: from imp-b07.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 18, 30 -- by steiner.cc.vt.edu (MOS 3.8.6-GA) 18, 30 -- with ESMTP id JHT26180; 18, 30 -- Mon, 2 Jun 2008 16:19:46 -0400 (EDT) 18, 30 -- Received: from imp-b07.cc.vt.edu (localhost.localdomain [127.0.0.1]) 18, 30 -- by imp-b07.cc.vt.edu (8.13.1/8.13.1) with ESMTP id m52KJkpr006528 18, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 16:19:46 -0400 18, 30 -- Received: (from apache-at-localhost) 18, 30 -- by imp-b07.cc.vt.edu (8.13.1/8.13.1/Submit) id m52KJk3j006527 18, 30 -- for Microscopy-at-microscopy.com; Mon, 2 Jun 2008 16:19:46 -0400 18, 30 -- Received: from us194px00-pat.tycoelectronics.net (us194px00-pat.tycoelectronics.net [198.175.154.212]) 18, 30 -- by webmail.vt.edu (IMP) with HTTP; Mon, 2 Jun 2008 16:19:45 -0400 18, 30 -- Message-ID: {1212437985.484455e1f24e3-at-webmail.vt.edu} 18, 30 -- Date: Mon, 2 Jun 2008 16:19:45 -0400 18, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} 18, 30 -- To: Microscopy-at-microscopy.com 18, 30 -- Subject: Re: [Microscopy] Re: FW: Re: Mitochondria query summary---Thanks!! (Long) 18, 30 -- References: {862904.82263.qm-at-web37404.mail.mud.yahoo.com} 18, 30 -- In-Reply-To: {862904.82263.qm-at-web37404.mail.mud.yahoo.com} 18, 30 -- MIME-Version: 1.0 18, 30 -- Content-Type: text/plain; charset=ISO-8859-1 18, 30 -- Content-Transfer-Encoding: 8bit 18, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
I appreciate your initiative, but I have to wonder why you think this will succeed. You are wise to consider these issues, and there are a lot of issues to consider.
Your EDS system may blithely kick out numbers for all elements, but they do not so quickly point out the limitations of the technique.
It would probably be bad enough trying to measure boron in steel. You also have a significant Nb content presenting you with an overlapping peak, so the problem gets worse. If the peaks can be deconvoluted there remain the issues of the matrix corrections.
You should probably record your own peak shapes for use as standards. That can probably be done quickly. It may not so easily be done well or right.
You should probably try replicate analyses and see if your system gives you repeatable results for different points of the same phase. I would also be concerned that the material may not be homogeneous. If it is not, that is one more complication.
You might go ahead and attempt the analysis, but I would look at the correction factors for B. They will be large and they will also be rather uncertain. I suppose your best results would be with using an iron-boron standard as a reference. You won't be able to use your FeCoNbB material as you will have both Nb and B contribution to the B peak.
You might try analyzing boron by difference. If it is the only element left out of the EDS analysis, it might be feasible. I tried such an analysis on a lithium-doped sample several years ago. I didn't totally trust the results, but they were at least showing up in the right ballpark (2-5%).
In short, this is not an exercise for the faint of heart or for the novice. I rather doubt that it will be possible. If you think you have been successful at it, you should be prepared to address quite a few skeptics. Still, I think EDS is a powerful technique and can be used to answer lots of questions when used correctly.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: celikaktas-at-gmail.com [mailto:celikaktas-at-gmail.com] Sent: Monday, June 02, 2008 3:10 AM To: wesaia-at-iastate.edu
Dear all,
Recently, I have been trying to analyze an alloy which was supposed to have the following composition:
Fe30Co32Nb8B30 by atomic percent.
Using EDS system attached to our SEM, I can detect Boron levels of about 6 at.% in this alloy.
This alloy has a mosaic structure, grains on the order of few micron. We are using a Bruker brand XFlash EDS detector. Also, Nb has an M-line peak position at an energy slightly lower than Boron peak position.
Given all these negative effects about quantitative EDS analysis for this particular sample; is it possible to undershoot Boron amount by such a big amount. Or can I suspect that in this alloy (the surface available in EDS analysis) the real Boron amount is much lower than initially thought?
By the way, I have put a piece of pure Boron to check the detection limit of our EDS detector. In relatively short time (2-3 minutes), I have obtained a very strong Boron peak (~15 cps/eV). Background at low energies was less than ~1 cps/eV.
Thank you for your suggestions, Ayten Celik-Aktas, PhD
==============================Original Headers============================== 22, 36 -- From wesaia-at-iastate.edu Mon Jun 2 17:55:26 2008 22, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 22, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m52MtQCr028970 22, 36 -- for {Microscopy-at-Microscopy.Com} ; Mon, 2 Jun 2008 17:55:26 -0500 22, 36 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 22, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m52MtPWM013975 22, 36 -- for {Microscopy-at-Microscopy.Com} ; Mon, 2 Jun 2008 17:55:25 -0500 22, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 22, 36 -- id 5afc_98bff20e_30f6_11dd_8c0e_00137253420a; 22, 36 -- Mon, 02 Jun 2008 17:52:37 -0500 22, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 22, 36 -- by despam-10.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m52MtERf005859 22, 36 -- for {Microscopy-at-Microscopy.Com} ; Mon, 2 Jun 2008 17:55:14 -0500 22, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 22, 36 -- Mon, 2 Jun 2008 17:55:21 -0500 22, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 22, 36 -- Content-class: urn:content-classes:message 22, 36 -- MIME-Version: 1.0 22, 36 -- Content-Type: text/plain; 22, 36 -- charset="us-ascii" 22, 36 -- Subject: RE: [Microscopy] Detecting Boron with EDS system 22, 36 -- Date: Mon, 2 Jun 2008 17:57:18 -0500 22, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D72702EAD572-at-maire.eng.iastate.edu} 22, 36 -- In-Reply-To: {200806020810.m528AQNU022519-at-ns.microscopy.com} 22, 36 -- X-MS-Has-Attach: 22, 36 -- X-MS-TNEF-Correlator: 22, 36 -- Thread-Topic: [Microscopy] Detecting Boron with EDS system 22, 36 -- Thread-Index: AcjEiB+5bT+/03wQRoyN/1KQagiJigASvxcg 22, 36 -- References: {200806020810.m528AQNU022519-at-ns.microscopy.com} 22, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 22, 36 -- To: {Microscopy-at-Microscopy.Com} 22, 36 -- X-OriginalArrivalTime: 02 Jun 2008 22:55:21.0148 (UTC) FILETIME=[BC1A67C0:01C8C503] 22, 36 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.6.2.154216 22, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P1_5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __pbl.spamhaus.org_TIMEOUT , __sbl.spamhaus.org_TIMEOUT ' 22, 36 -- Content-Transfer-Encoding: 8bit 22, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m52MtQCr028970 ==============================End of - Headers==============================
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Question: Does someone in the community have some experience/opinion about the osmium coating for FE-SEM as a replacement for conventional gold coating?. Any particular comment about Os vs Au or Au/Pd or Pd/Pt for biological specimens (collagen, cells, etc)?
Consider Pd and Ir. If EDS is not an issue, these will work well. The caveat is to coat at high vacuum. This is typically about 15-20mT. With rotation, you can get a very nice coating. At high mag, Au/Pd and Au are seen. Pt works well too at high vac.
gary g.
At 06:04 PM 6/2/2008, you wrote:
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==============================Original Headers============================== 6, 20 -- From gary-at-gaugler.com Mon Jun 2 21:20:11 2008 6, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m532KBcL028676 6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 21:20:11 -0500 6, 20 -- Message-Id: {200806030220.m532KBcL028676-at-ns.microscopy.com} 6, 20 -- Received: (qmail 7428 invoked from network); 2 Jun 2008 19:20:10 -0700 6, 20 -- Received: by simscan 1.1.0 ppid: 7422, pid: 7424, t: 0.1179s 6, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 6, 20 -- by smtp1 with SMTP; 2 Jun 2008 19:20:09 -0700 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 20 -- Date: Mon, 02 Jun 2008 19:20:05 -0700 6, 20 -- To: acamacho-at-rd.us.loreal.com 6, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 20 -- Subject: Re: [Microscopy] viaWWW: Coatings for SEM 6, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 6, 20 -- In-Reply-To: {200806030104.m5314C28015817-at-ns.microscopy.com} 6, 20 -- References: {200806030104.m5314C28015817-at-ns.microscopy.com} 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-22A65FC2 ==============================End of - Headers==============================
Two cents more, even if Scott has listed most of what can be said.
A first an easy step can be to test the homogeneity of your sample, what can be done by comparison, measuring only the peak intensity variations. It's an easy step which will give you much information about your sample. A good relative measurement is much more worth than an bad or uncertain "quantitative" one. If you have some other samples from the same family, with known different compositions, you may be able to place the unknown beside the others. It's a good step.
If you need quantitative measurement, as Scott point out, you must work with your one peak shapes, particulary for low energy peaks and if overlapping occurs, and do the analysis with standard. If your system aloud it, use 5 eV or 2 eV per channel as X resolution (in relation with the following point). This will help for the deconvolution.
An other point is the choice of your primary energy, which will have much effect on the relative intensity of the lines, on the computing of the absorbtion correction and on the depth of the analysed volume (which will of coarse not be the same for B-Ka and Nb-L). You could have better results working with the L lines of Co, Fe and Nb at a primary energy of something like 5-8 keV, than using the Fe and Co K lines, which need 12-15 keV, what is much too high for boron. At lower energy, the B-k line will be stronger, the Nb-L too of coarse, but this will be a help for the deconvolution. Of coarse, the overlapping of Fe and Co L will be a new difficulty, but with 2 eV per channel, and 30% in composition for each, it schould not be the biggest one. And Fe-Kb overlaps too with Co-Ka.
Last, as a test of what your syteme is computing, you can try to make the same analysis, at different energies. The same point/grain of your sample, same xray lines (Fe and Co K), same counting rate (not always possible), but different energies, say 20, 15, 10, 8. Are the calculated concentrations varying much with the primary energy ? What about the absorbtion coefficient ? Etc. You could have some diagnosic of the "black box " of your system.
But as soon said, it's a difficult job.
Hope it helps
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
celikaktas-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } Recently, I have been trying to analyze an alloy which was supposed to } have the following composition: } } Fe30Co32Nb8B30 by atomic percent. } } Using EDS system attached to our SEM, I can detect Boron levels of } about 6 at.% in this alloy. } } This alloy has a mosaic structure, grains on the order of few micron. } We are using a Bruker brand XFlash EDS detector. Also, Nb has an } M-line peak position at an energy slightly lower than Boron peak } position. } } Given all these negative effects about quantitative EDS analysis for } this particular sample; is it possible to undershoot Boron amount by } such a big amount. Or can I suspect that in this alloy (the surface } available in EDS analysis) the real Boron amount is much lower than } initially thought? } } By the way, I have put a piece of pure Boron to check the detection } limit of our EDS detector. In relatively short time (2-3 minutes), I } have obtained a very strong Boron peak (~15 cps/eV). Background at low } energies was less than ~1 cps/eV. } } } Thank you for your suggestions, } Ayten Celik-Aktas, PhD } } ==============================Original Headers============================== } 9, 27 -- From celikaktas-at-gmail.com Mon Jun 2 03:09:22 2008 } 9, 27 -- Received: from fk-out-0910.google.com (fk-out-0910.google.com [209.85.128.187]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5289MYs021381 } 9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 2 Jun 2008 03:09:22 -0500 } 9, 27 -- Received: by fk-out-0910.google.com with SMTP id 18so895076fks.2 } 9, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 02 Jun 2008 01:09:21 -0700 (PDT) } 9, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 27 -- d=gmail.com; s=gamma; } 9, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 9, 27 -- bh=G+xm0xeDkTf/F7QfsOBm5xiYWMamSp36s9CTOEyq6CI=; } 9, 27 -- b=OZREQCzOTEwoccQV++BbW0usafzK4GYuSZhVOQh/zGcnKm76E9T/QxBd8Hx3EE7XyWB8zWcFmpBLHlaZXiir3tZnDJdHI7MNY6ZpUdolQ7cbgk8mV+RHp7o1uKDx8W2Q/tqToUcV1GGsuPzbjF9CX62O0DY21iK9LHFolIcqjtQ= } 9, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 9, 27 -- d=gmail.com; s=gamma; } 9, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 9, 27 -- b=QN8qjvGGTKBYbFZs0Rabgu6XFGnYT/qMswHeDrGG6Vy4wT83jDfyzckZY+O+XC3dTaBYt2bOLg7GbLUg3wLcKnuzx2RNpQU/8du2vLR+fKeyPqoNSxULS0/bNt1BU/5lOlqcm9TWgcHUpDfkULLlL1vaMW/QwQBsjavO2mVeD+U= } 9, 27 -- Received: by 10.82.151.9 with SMTP id y9mr328047bud.8.1212394161151; } 9, 27 -- Mon, 02 Jun 2008 01:09:21 -0700 (PDT) } 9, 27 -- Received: by 10.82.125.7 with HTTP; Mon, 2 Jun 2008 01:09:21 -0700 (PDT) } 9, 27 -- Message-ID: {1075c5c10806020109i4255efa1ya59bf2684ee8d091-at-mail.gmail.com} } 9, 27 -- Date: Mon, 2 Jun 2008 11:09:21 +0300 } 9, 27 -- From: "Ayten Celik-Aktas" {celikaktas-at-gmail.com} } 9, 27 -- To: microscopy {Microscopy-at-microscopy.com} } 9, 27 -- Subject: Detecting Boron with EDS system } 9, 27 -- MIME-Version: 1.0 } 9, 27 -- Content-Type: text/plain; charset=UTF-8 } 9, 27 -- Content-Transfer-Encoding: 7bit } 9, 27 -- Content-Disposition: inline } ==============================End of - Headers============================== }
==============================Original Headers============================== 15, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Jun 3 04:36:55 2008 15, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.155]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m539askc023564 15, 29 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 04:36:55 -0500 15, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 15, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m539aq5c025338 15, 29 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 11:36:53 +0200 (CEST) 15, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 15, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 1E441108B3EC 15, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 3 Jun 2008 11:36:14 +0200 (CEST) 15, 29 -- Message-ID: {4845108E.4020800-at-ipcms.u-strasbg.fr} 15, 29 -- Date: Tue, 03 Jun 2008 11:36:14 +0200 15, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 15, 29 -- User-Agent: Thunderbird 1.5.0.14ubu (X11/20080502) 15, 29 -- MIME-Version: 1.0 15, 29 -- To: Microscopy-at-microscopy.com 15, 29 -- Subject: Re: [Microscopy] Detecting Boron with EDS system 15, 29 -- References: {200806020818.m528IQio029460-at-ns.microscopy.com} 15, 29 -- In-Reply-To: {200806020818.m528IQio029460-at-ns.microscopy.com} 15, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-IPCMS-MailScanner: Found to be clean 15, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 15, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.155]); Tue, 03 Jun 2008 11:36:53 +0200 (CEST) 15, 29 -- X-Virus-Scanned: ClamAV 0.93/6668/Tue Apr 8 13:57:49 2008 on mr5.u-strasbg.fr 15, 29 -- X-Virus-Status: Clean 15, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=disabled 15, 29 -- version=3.2.4 15, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on mr5.u-strasbg.fr ==============================End of - Headers==============================
One re-read always before sending, but ... A little correction.
I wrote : "At lower energy, the B-k line will be stronger, the Nb-L too of coarse". Of coarse, it's the Nb-M and not the Nb-L, in that case. Sure, you have all automatically correct the miss-writing !
Jacques
--
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
A 'non-tourist' has apparently been teaching the wrong answer since this question is posed as an exercise in Baddeley and Jensen's book. The problem asks the reader to show that that the answer is no despite the claims of others. In fact, they recommend finding a population in which the estimated diameter is larger than the actual diameter.
The answer to question 2 is no unless the population distribution is known.
In other words you already have to know the sizes to determine the sizes. Not a good situation.
Think of it this way, if all of the spherical mitochondria were the same size, then the profiles that are formed are almost always smaller than the true diameter. There is a means of determining the diameter of this population from the data, and also a means of determining the volume.
It is not possible to compare populations of mitochondria by comparing the areas. There are many ways in which changes in area could be caused by different factors: 1. Changes in all mitochondria size 2. Changes in some mitochondria size 3. Changes in mitochondria shape 4. Changes in mitochondria orientation ...
So an observed change in mean area could be due to a change in the population distribution. Or it might be due to a change in the shape of the mitochondria. So it might be due to an actual change or to a sectioning artifact.
As I said in the first message, a profile is what we see on a section. No one is really interested in information about the profile. What is of interest is information about the original 3D objects. As we see in this simple question in which ideal shapes are used, information such as areas and diameters seen on the section, may not be relevant to the original 3D structures being studied. This is the reason stereology was founded as its own science.
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 9, 28 -- From rboehrin-at-vt.edu Tue Jun 3 10:23:36 2008 9, 28 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m53FNZE0010775 9, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 10:23:35 -0500 9, 28 -- Received: from vivi.cc.vt.edu (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) 9, 28 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id m53FNZsB025814 9, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 11:23:35 -0400 9, 28 -- Received: from imp-b06.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 9, 28 -- by vivi.cc.vt.edu (MOS 3.8.6-GA) 9, 28 -- with ESMTP id JLR96555; 9, 28 -- Tue, 3 Jun 2008 11:23:35 -0400 (EDT) 9, 28 -- Received: from imp-b06.cc.vt.edu (localhost.localdomain [127.0.0.1]) 9, 28 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1) with ESMTP id m53FNZKb029167 9, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 11:23:35 -0400 9, 28 -- Received: (from apache-at-localhost) 9, 28 -- by imp-b06.cc.vt.edu (8.13.1/8.13.1/Submit) id m53FNYLD029166 9, 28 -- for Microscopy-at-microscopy.com; Tue, 3 Jun 2008 11:23:34 -0400 9, 28 -- Received: from us194px00-pat.tycoelectronics.net (us194px00-pat.tycoelectronics.net [198.175.154.212]) 9, 28 -- by webmail.vt.edu (IMP) with HTTP; Tue, 3 Jun 2008 11:23:34 -0400 9, 28 -- Message-ID: {1212506614.484561f6c10af-at-webmail.vt.edu} 9, 28 -- Date: Tue, 3 Jun 2008 11:23:34 -0400 9, 28 -- From: Robert Boehringer {rboehrin-at-vt.edu} 9, 28 -- To: Microscopy-at-microscopy.com 9, 28 -- Subject: Re: Mitochondria query summary---Thanks!! (Long) 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Type: text/plain; charset=ISO-8859-1 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
I am working on a project that attempts to evaluate the effects of choices made in EMPA on the data and ultimately on the geological interpretations. I want to analyze a set of geological specimens using three, four, maybe five analytical schemes, and I'll see how those different schemes affect the data and ultimate interpretations. Two or three of these schemes will be based on common approaches in the relevant literature. The main goal is essentially to determine the robustness of the geological interpretations. Can these interpretations withstand variations introduced by different analysts? Are the ultimate interpretations vulnerable to the different "cultures" or analytical "traditions" of different labs?
Analysts carry out a series of actions and make a series of choices while doing an analysis. This is considered an "operational sequence" by anthropologists who study technology, and such anthropologists are interested in the choices people make and the reasons for those choices. Some of these choices, even in EMPA, are made for very technical and logical reasons (e.g., an accelerating voltage of 15 kV optimizes the overvoltage ratio for the main elements of interest), whereas other choices are made for outdated, arbitrary, or other reasons (e.g., we use Correction Method A, not Correction Method B, in this lab because it is what we've always done). Both types of choices can be equally important in obtaining accurate data.
Back to my research project, I have ideas about what choices are most important and common in EMPA when deciding how to analyze a geological specimen. But I'd like to not bias my project by investigating only my own ideas about important and common choices. I haven't spent very much time in other microprobe labs, so I am embedded in the "culture" of the lab here at the University of Minnesota.
So I'd like to hear from my colleagues on this issue. What do you think are the most important and common choices made when approaching an analysis? Are there choices that you think almost all of your colleagues would make the same way? Have researchers from other labs asked you to use conditions that boggle you? If you have spent a lot of time in multiple labs, have you recognized the different "cultures" of the labs? What choices do you think analysts make that aren't really important today but are based on habits or traditions from older machines or correction routines? I will use any comments and criticisms I receive to select the choices on which this project should focus, probably just by tabulating the responses.
I apologize for being a bit vague, but I'm trying not to bias anyone's responses. I welcome all input and opinions either on- or off-list. All comments or criticisms will remain anonymous unless you either respond on-list or give me explicit permission to cite you.
Thank you for your time and assistance. I apologize if you have received this email more than once due to cross-list posting.
Best, Ellery
-------------------- Ellery E. Frahm Research Fellow & Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu Personal Website: http://umn.edu/~frah0010
==============================Original Headers============================== 9, 17 -- From frah0010-at-umn.edu Tue Jun 3 21:49:47 2008 9, 17 -- Received: from mta-a2.tc.umn.edu (mta-a2.tc.umn.edu [134.84.119.206]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m542nl8k023728 9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 21:49:47 -0500 9, 17 -- Received: from [10.0.1.8] (c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206]) 9, 17 -- by mta-a2.tc.umn.edu (UMN smtpd) with ESMTP 9, 17 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jun 2008 21:49:47 -0500 (CDT) 9, 17 -- X-Umn-Remote-Mta: [N] c-75-72-182-206.hsd1.mn.comcast.net [75.72.182.206] #+TS+AU+HN 9, 17 -- Message-Id: {7D8CD1F2-31F0-4C75-96E8-8ED4FBF3BD38-at-umn.edu} 9, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} 9, 17 -- To: microscopy-at-microscopy.com 9, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 17 -- Content-Transfer-Encoding: 7bit 9, 17 -- Mime-Version: 1.0 (Apple Message framework v924) 9, 17 -- Subject: Choices in EMPA 9, 17 -- Date: Tue, 3 Jun 2008 21:49:47 -0500 9, 17 -- X-Mailer: Apple Mail (2.924) ==============================End of - Headers==============================
I have a Cambridge S200. When selecting TV mode, the screen goes blank. All other video modes work correctly.
It appears that the video signal in TV mode is getting through, but it is being blanked. We have traced the blanking to the 853100 board and TP15 which shows the blanking pulses. In all the other modes the pulses look great. In TV mode it is TTL high, cutting off the video signal. I did note that removing jumper LK3 allows blanking pulses, but the video rate still seems to be 15 frames per second.
We have no schematic on this board and I am looking both for clues as to what to check as well as a schematic.
Any help would be appreciated.....
-Dr. Mike Brown Science Dept Chair AAEC-PV
==============================Original Headers============================== 4, 17 -- From mbrown-at-aaechighschools.com Wed Jun 4 19:23:49 2008 4, 17 -- Received: from smtpoutwbe02.prod.mesa1.secureserver.net (smtpoutwbe02.prod.mesa1.secureserver.net [208.109.78.113]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m550Nljl018380 4, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 4 Jun 2008 19:23:48 -0500 4, 17 -- Received: (qmail 622 invoked from network); 5 Jun 2008 00:23:43 -0000 4, 17 -- Received: from unknown (HELO gem-wbe04.prod.mesa1.secureserver.net) (64.202.189.36) 4, 17 -- by smtpoutwbe02.prod.mesa1.secureserver.net with SMTP; 5 Jun 2008 00:23:43 -0000 4, 17 -- Received: (qmail 21580 invoked by uid 99); 5 Jun 2008 00:23:43 -0000 4, 17 -- Date: Wed, 04 Jun 2008 17:23:43 -0700 4, 17 -- From: mbrown-at-aaechighschools.com 4, 17 -- Subject: Trouble with TV mode on Cambridge S200 SEM 4, 17 -- To: Microscopy-at-Microscopy.Com 4, 17 -- Message-ID: {20080604172343.889a5134facffa13190a02200f773d01.22377e0dc6.wbe-at-email.secureserver.net} 4, 17 -- MIME-Version: 1.0 4, 17 -- Content-Type: TEXT/plain; CHARSET=US-ASCII 4, 17 -- User-Agent: Web-Based Email 4.13.2 4, 17 -- X-Originating-IP: 63.229.121.32 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Employment Opportunity in Richmond, Virginia
Question: Have you experience with the TEM characterization of asbestos? Would you like to set up your own TEM suite within a contract lab looking to become NVLAP certified? If you have an advanced degree, more than five years in a Supervisory role characterizing fibers, know what it takes to set up a lab and have an entrepreneurial spirit, please contact Sheila at 804-648-0267.
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Email: jwdeng-at-imr.ac.cn Name: Deng
Title-Subject: [Filtered] a small TEM Objective Aperture
Question: Dear All Our research institute has a Tecnai F30 transmission electron microscopy and we want to do some Dark Field observation on it. So we are in need of an Objective Aperture (OA) as small as possible. In our microscopy a metal strip contains 7 apertures and the smallest OA size is 10 micrometer. Has anyone once bought or fabricated a small OA like that? Can you give me some information like the company, corresponding address, price and so on.
Thanks in advance for any advice or helpful hints.
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Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] TEM - low Z grids
Question: All,
We are using EDS in the TEM to analyze catalyst powders for low concentrations of Pd, Pt, Ni & Re. We've been using 300 mesh Cu grids that are fomvar coated with lacy carbon but the Cu Ka lines interfere with the analysis.
We have found carbon grids but they must be purchased in lots of 100 at great cost. We only need a dozen. Is there another, affordable, solution?
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Email: ecoagripolicy-at-gmail.com Name: Rachel
Organization: UWA
Title-Subject: [Filtered] Request for papers
Question: Hi All, Please if anyone can find the following journal articles/ copy pages of the books please can you hunt it for me send either a hard/soft copy?
Ziegenspeck, H. (1949). Die Emission polarisierten Fluoreszenzlichtes (Difluoreszenz) durch gef”rbte Zellulose und Kutinmembranen von Pflanzen. In F. Bra?utigam & A. Grabner (Eds.), Beitrage zur Fluoreszenzmikroskopie (pp. 71-85). Wien : Fromme. (Special issue of "Mikroskopie")
Isings, J. (1966). Combined fluorescence dichroism and polarization microscopy of cotton fibres under stress. Microscope and Crystal Front (Carshalton Beeches (Surrey)), 15(2), 71-79
Luhan M, 1947. Die Goldendodermis der Farne. Fluoreszenmikroskopische untersuchungen zur vergleichenden Anatomie der Filicineen. Sitzungsberichte der Osterreichische Akademie der Wissenschaften Abteilung I. 156, 1-56.
Klein G and H Linser. 1930. Fluoreszenzanalytische untersuchungen an pflanze. Osterreische botanische zeitschrift. 79, 125-163.
I will be using the papers solely for my research/study purpose only and will not be distributing, if there is any copyright issues.
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Email: bjulies-at-uwc.ac.za Name: Basil Julies
Organization: University of the Western Cape
Title-Subject: [Filtered] Post in South Africa : EM operator for tecnai F20 TEM on senior lecturer level
Question: The Electron Microscope Unit (EMU) at the University of the Western Cape (UWC), South Africa (SA), has recently acquired a new FEI Tecnai F20 X-Twin MAT TEM with all the bells and whistles. This is a golden opportunity for a postdoc who already has extensive experience in operating a FEG-TEM, and understands the theory and technique of high resolution TEM and STEM, as well as EELS, to take up a permanent academic position in the Electron Microscope Unit (EMU) at the University of the Western Cape.
The EMU is a research facility in the Faculty of Natural Sciences which offers training for and assistance to staff and postgraduate students who wish to pursue scientific research using the electron microscopes and related equipment.
The incumbent will be required to manage the tecnai F20 and the related sample preparation processes, and provide the necessary training and assistance to the users in acquiring, analysing and interpreting high resolution images taken on the TEM. In addition, the incumbent will also be required to do some lecturing by way of short courses on Electron Microscopy, supervise students, and assist with maintenance and minor administration duties.
For further information on this permanent academic post on a senior lecturer level, please contact Dr Basil Julies on tel number +27 21 959 2327 or at bjulies-at-uwc.ac.za
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==============================Original Headers============================== 18, 27 -- From jd-at-laddresearch.com Thu Jun 5 14:06:05 2008 18, 27 -- Received: from talbot.electric.net (talbot.electric.net [72.35.23.19]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m55J651E022841 18, 27 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 14:06:05 -0500 18, 27 -- Received: from 1K4Kmd-0003zp-VR by talbot.electric.net with emc1-ok (Exim 4.67) 18, 27 -- (envelope-from {jd-at-laddresearch.com} ) 18, 27 -- id 1K4Kme-00041t-V0; Thu, 05 Jun 2008 12:06:00 -0700 18, 27 -- Received: by emcmailer; Thu, 05 Jun 2008 12:06:00 -0700 18, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 18, 27 -- by talbot.electric.net with esmtps (TLSv1:AES256-SHA:256) 18, 27 -- (Exim 4.67) 18, 27 -- (envelope-from {jd-at-laddresearch.com} ) 18, 27 -- id 1K4Kmd-0003zp-VR; Thu, 05 Jun 2008 12:06:00 -0700 18, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 18, 27 -- Date: Thu, 05 Jun 2008 15:04:06 -0400 18, 27 -- To: jwdeng-at-imr.ac.cn 18, 27 -- From: jd {jd-at-laddresearch.com} 18, 27 -- Subject: Re: [Microscopy] viaWWW: a small TEM Objective Aperture 18, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 18, 27 -- In-Reply-To: {200806050054.m550swti001267-at-ns.microscopy.com} 18, 27 -- References: {200806050054.m550swti001267-at-ns.microscopy.com} 18, 27 -- Mime-Version: 1.0 18, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 18, 27 -- X-Outbound-IP: 216.204.198.170 18, 27 -- X-Env-From: jd-at-laddresearch.com 18, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 18, 27 -- Message-Id: {E1K4Kme-00041t-V0-at-talbot.electric.net} ==============================End of - Headers==============================
I'm setting up an infra-red imaging system to image motion of zebrafish larvae for a rheotaxis study. this involves an IR illumination outside the sensitivity range of the fish, roughly } 650 nm in order to prevent visual cues. Does anyone know the wavelengths that pass through a Kodak No. 2 safelight filter? There are other options for illumination, such as LEDs, but this safelight is sitting on the shelf. Kodak has been of no assistance.
Thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 8, 24 -- From glenmac-at-u.washington.edu Thu Jun 5 15:11:08 2008 8, 24 -- Received: from mxout3.cac.washington.edu (mxout3.cac.washington.edu [140.142.32.166]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m55KB74c005692 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 15:11:08 -0500 8, 24 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9] (may be forged)) 8, 24 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m55KB7Hg007659 8, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 13:11:07 -0700 8, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 8, 24 -- (authenticated authid=glenmac) 8, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m55KB7OP014472 8, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 13:11:07 -0700 8, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- Message-Id: {9B9028C0-F935-474D-A6FB-04A987AF3C69-at-u.washington.edu} 8, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 8, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 8, 24 -- Subject: Safelight wavelength 8, 24 -- Date: Thu, 5 Jun 2008 13:11:05 -0700 8, 24 -- X-Mailer: Apple Mail (2.753) 8, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.6.5.125630 8, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_900_999 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Thu Jun 5 15:52:02 2008 10, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m55Kq1sb019878 10, 20 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 15:52:01 -0500 10, 20 -- Message-Id: {200806052052.m55Kq1sb019878-at-ns.microscopy.com} 10, 20 -- Received: (qmail 30413 invoked from network); 5 Jun 2008 13:51:59 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 30409, pid: 30411, t: 0.1106s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 20 -- by smtp1 with SMTP; 5 Jun 2008 13:51:59 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Thu, 05 Jun 2008 13:51:58 -0700 10, 20 -- To: glenmac-at-u.washington.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Safelight wavelength 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200806052012.m55KCl6F008243-at-ns.microscopy.com} 10, 20 -- References: {200806052012.m55KCl6F008243-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-554951EC ==============================End of - Headers==============================
Thanks for the information, Dale sent a Kodak document that contained the necessary transmittance curves, and the #2 is a long pass that cuts on at about 650 nm. Perfect for our needs. The #7B is a green filter with a low intensity, 40 nm wide notch centered 540 nm for use with IR sensitive film that would be affected by something like the #2.
Regards, and thanks again, Glen
On Jun 5, 2008, at 1:51 PM, Gary Gaugler wrote:
} I think you want 7B filter. } } http://www.kodak.com/US/plugins/acrobat/en/motion/products/filter/ } K4_Safelight_1106.pdf } } gary g. } } } At 01:12 PM 6/5/2008, you wrote: } } Hi Glen. } } I'm sending this to you directly since the List doesn't accept } attachments. Since we are supposed to reply to the List, can you } please send to the List and tell folks you got the info? } } Hope this is what you needed. } } Dale } } } } --------------------------------------------------------------------- } } ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } ------- } } } } I'm setting up an infra-red imaging system to image motion of } } zebrafish larvae for a rheotaxis study. this involves an IR } } illumination outside the sensitivity range of the fish, roughly } 650 } } nm in order to prevent visual cues. Does anyone know the wavelengths } } that pass through a Kodak No. 2 safelight filter? There are other } } options for illumination, such as LEDs, but this safelight is sitting } } on the shelf. Kodak has been of no assistance. } } } } Thanks, } } Glen } } } } } } } } Glen MacDonald } } Core for Communication Research } } Virginia Merrill Bloedel Hearing Research Center } } Box 357923 } } University of Washington } } Seattle, WA 98195-7923 USA } } (206) 616-4156 } } glenmac-at-u.washington.edu } } } } ********************************************************************* } } *** } } ****** } } The box said "Requires WindowsXP or better", so I bought a Macintosh. } } ********************************************************************* } } *** } } ****** } } } } } } } } ==============================Original } } Headers============================== } } 8, 24 -- From glenmac-at-u.washington.edu Thu Jun 5 15:11:08 2008 } } 8, 24 -- Received: from mxout3.cac.washington.edu } } (mxout3.cac.washington.edu [140.142.32.166]) } } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id m55KB74c005692 } } 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 } } 15:11:08 -0500 } } 8, 24 -- Received: from smtp.washington.edu (smtp.washington.edu } } [140.142.33.9] (may be forged)) } } 8, 24 -- by mxout3.cac.washington.edu (8.13.7+UW06.06/8.13.7 } } +UW07.09) with ESMTP id m55KB7Hg007659 } } 8, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } } bits=256 verify=OK) } } 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 } } 13:11:07 -0700 } } 8, 24 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) } } 8, 24 -- (authenticated authid=glenmac) } } 8, 24 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7 } } +UW07.09) with ESMTP id m55KB7OP014472 } } 8, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 } } verify=NOT) } } 8, 24 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 } } 13:11:07 -0700 } } 8, 24 -- Mime-Version: 1.0 (Apple Message framework v753) } } 8, 24 -- Content-Transfer-Encoding: 7bit } } 8, 24 -- Message-Id: {9B9028C0-F935-474D- } } A6FB-04A987AF3C69-at-u.washington.edu} } } 8, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } } format=flowed } } 8, 24 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" } } {microscopy-at-microscopy.com} } } 8, 24 -- From: Glen MacDonald {glenmac-at-u.washington.edu} } } 8, 24 -- Subject: Safelight wavelength } } 8, 24 -- Date: Thu, 5 Jun 2008 13:11:05 -0700 } } 8, 24 -- X-Mailer: Apple Mail (2.753) } } 8, 24 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: } } 2.6.0.325393, Antispam-Data: 2008.6.5.125630 } } 8, 24 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, } } Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_5000_LESS 0, } } BODY_SIZE_900_999 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CT 0, } } __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, } } __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' } } ==============================End of - } } Headers============================== }
==============================Original Headers============================== 6, 26 -- From glenmac-at-u.washington.edu Thu Jun 5 18:56:32 2008 6, 26 -- Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m55NuWfB005910 6, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 18:56:32 -0500 6, 26 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9] (may be forged)) 6, 26 -- by mxout1.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m55NuVPg003516 6, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 6, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 16:56:31 -0700 6, 26 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 6, 26 -- (authenticated authid=glenmac) 6, 26 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id m55NuVU1030443 6, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 6, 26 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jun 2008 16:56:31 -0700 6, 26 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 26 -- In-Reply-To: {200806052052.m55Kq3YP001666-at-cg72.u.washington.edu} 6, 26 -- References: {200806052012.m55KCl6F008243-at-ns.microscopy.com} {200806052052.m55Kq3YP001666-at-cg72.u.washington.edu} 6, 26 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 26 -- Message-Id: {2D5B8A79-79B8-4D19-A81A-357953C4C06F-at-u.washington.edu} 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 6, 26 -- Subject: Re: [Microscopy] Safelight wavelength 6, 26 -- Date: Thu, 5 Jun 2008 16:56:30 -0700 6, 26 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 6, 26 -- X-Mailer: Apple Mail (2.753) 6, 26 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.6.5.164444 6, 26 -- X-Uwash-Spam: Gauge=X, Probability=10%, Report='LINES_OF_YELLING_3 0.671, BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __LINES_OF_YELLING 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' ==============================End of - Headers==============================
Dear Owen, In the past I have purchased beryllium grids, one at a time. They are expensive but give no background at all. I have also purchased carbon-coated nylon grids. The grids tend to burn in a 200kV TEM if you hit the grid with the beam, but they are cheap and have a low background. Check the EM supply houses. Regards,
-----Original Message----- X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu] Sent: June 4, 2008 5:57 PM To: maryflet-at-interchange.ubc.ca
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Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] TEM - low Z grids
Question: All,
We are using EDS in the TEM to analyze catalyst powders for low concentrations of Pd, Pt, Ni & Re. We've been using 300 mesh Cu grids that are fomvar coated with lacy carbon but the Cu Ka lines interfere with the analysis.
We have found carbon grids but they must be purchased in lots of 100 at great cost. We only need a dozen. Is there another, affordable, solution?
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REQUIREMENTS: * Bachelors Degree in Engineering, Physics or related discipline. * Solid technical understanding of electron beam operation and applications. * Minimum 3 years experience with an ebeam application. * Strong record of accomplishment. * Fine tuned organizational and follow-up skills. * Analytical, creative and pro-active problem solving skills. * Excellent communication, interpersonal and team building skills. * Self-motivated, achievement oriented, hands-on style. * Entrepreneurial attitude and spirit. * General computer skills. * Unquestionable integrity.
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==============================Original Headers============================== 11, 20 -- From mls1-at-ebsciences.com Fri Jun 6 11:04:50 2008 11, 20 -- Received: from mail.ebsciences.com (mail.ebsciences.com [65.115.7.18]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m56G4mlw010008 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jun 2008 11:04:49 -0500 11, 20 -- Received: from mnesta.ebsciences.private ([10.10.0.117]) 11, 20 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 11, 20 -- (Exim 4.69) 11, 20 -- (envelope-from {mls1-at-ebsciences.com} ) 11, 20 -- id 1K4eQp-0006CJ-6g 11, 20 -- for microscopy-at-microscopy.com; Fri, 06 Jun 2008 12:04:47 -0400 11, 20 -- Message-ID: {4849601D.3040503-at-ebsciences.com} 11, 20 -- Date: Fri, 06 Jun 2008 12:04:45 -0400 11, 20 -- From: Mike Nesta {mls1-at-ebsciences.com} 11, 20 -- Organization: Energy Beam Sciences 11, 20 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 11, 20 -- MIME-Version: 1.0 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- Subject: Energy Beam Sciences Employment Opportunity 11, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
In March I received a letter stating that Leica-Microsystems had acquired Bal-Tec AG. Leica support told me today that the former reps would still be handling sales of consumables, but emails to our former Bal-Tec rep directly and via links on the Bal-Tec website have bounced. I have also noticed that the Leica-Microsystems website lists some of the Balzers/Baltec equipment models but not the Jet Freeze Device JFD-030 and other products that may overlap with existing Leica products.
Does anyone know more details of their plans and who can sell us consumables for the Balzers/Bal-Tec gear (jet freeze sample holders, ebeam gun supplies?
Thanks,
Dale Callaham
==============================Original Headers============================== 8, 20 -- From dac-at-research.umass.edu Fri Jun 6 16:06:05 2008 8, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m56L65gR003212 8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Jun 2008 16:06:05 -0500 8, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 20 -- (authenticated bits=0) 8, 20 -- by race5.oit.umass.edu (8.14.3/8.14.1) with ESMTP id m56L64YY005327 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 6 Jun 2008 17:06:04 -0400 8, 20 -- Message-ID: {4849B53E.8030403-at-research.umass.edu} 8, 20 -- Date: Fri, 06 Jun 2008 17:07:58 -0500 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 20 -- Reply-To: dac-at-research.umass.edu 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 20 -- Subject: Status of Bal-Tec under Leica acquisition? 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
How long does 35mm film last? I have sealed individual rolls (Kodak and AGFA) expiration date 04/2001 and other older bulk rolls in their bulk loaders.
All have been kept in a drawer at room temperature. Are they still OK to use?
Thanks in advance,
Paula :-)
Paula Sicurello
VA Hospital San Diego
Microscope Facility room B141
3350 La Jolla Village Drive
San Diego, CA 92161
858-552-8585 x2397
==============================Original Headers============================== 14, 24 -- From vapatpxs-at-yahoo.com Mon Jun 9 12:30:38 2008 14, 24 -- Received: from n25.bullet.mail.mud.yahoo.com (n25.bullet.mail.mud.yahoo.com [68.142.206.220]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m59HUcZA010269 14, 24 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 12:30:38 -0500 14, 24 -- Received: from [68.142.200.221] by n25.bullet.mail.mud.yahoo.com with NNFMP; 09 Jun 2008 17:30:37 -0000 14, 24 -- Received: from [216.252.122.217] by t9.bullet.mud.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- Received: from [69.147.65.157] by t2.bullet.sp1.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- Received: from [127.0.0.1] by omp405.mail.sp1.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- X-Yahoo-Newman-Property: ymail-3 14, 24 -- X-Yahoo-Newman-Id: 373802.7282.bm-at-omp405.mail.sp1.yahoo.com 14, 24 -- Received: (qmail 52867 invoked by uid 60001); 9 Jun 2008 17:30:38 -0000 14, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 24 -- s=s1024; d=yahoo.com; 14, 24 -- h=Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 14, 24 -- b=MmN8GpdqMPfkJ8e1Y13HohpNQMOpWjzKhvDAKy6iQPq621WBRFjwM2JSPQdvPjBJapl8VrreH4f6KLJ1+4DJcUkjviL94smRv0c9t6mt6SNrNJt5V8vUxipA3c5HyAzLXJ/0Lm0kVCNuFt7AvL4Alzh87wcEIvm7CawsSKXwatg=; 14, 24 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Mon, 09 Jun 2008 10:30:38 PDT 14, 24 -- X-Mailer: YahooMailWebService/0.7.199 14, 24 -- Date: Mon, 9 Jun 2008 10:30:38 -0700 (PDT) 14, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 14, 24 -- Subject: 35mm film expiration date 14, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 14, 24 -- MIME-Version: 1.0 14, 24 -- Content-Type: text/plain; charset=us-ascii 14, 24 -- Message-ID: {183151.50499.qm-at-web46101.mail.sp1.yahoo.com} ==============================End of - Headers==============================
It's the room temperature part that bothers me. I have used films for years after the expiration date, if they were kept frozen and tightly sealed, but I would expect that you would see some significant deterioration after room temp storage that far after expiration. BW film would possibly show background fog (can't remember the technical term today) which would lower contrast. Color film would probably be color-shifted and somewhat fogged, too.
My approach would be to take a couple rolls, shoot half of them of some meaningful target (color chart or something) and leave the other half clear. Have them processed and see if there's bad stuff on the unexposed frames and how it might be affecting your actual images.
Since this is all dependent on film type, film speeds, etc., I'm guessing here, but It may be okay for non-critical use, but not much more.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] Sent: Monday, June 09, 2008 12:32 PM To: Tindall, Randy D.
Hello Listers,
How long does 35mm film last? I have sealed individual rolls (Kodak and AGFA) expiration date 04/2001 and other older bulk rolls in their bulk loaders.
All have been kept in a drawer at room temperature. Are they still OK to use?
Thanks in advance,
Paula :-)
Paula Sicurello
VA Hospital San Diego
Microscope Facility room B141
3350 La Jolla Village Drive
San Diego, CA 92161
858-552-8585 x2397
==============================Original Headers============================== 14, 24 -- From vapatpxs-at-yahoo.com Mon Jun 9 12:30:38 2008 14, 24 -- Received: from n25.bullet.mail.mud.yahoo.com (n25.bullet.mail.mud.yahoo.com [68.142.206.220]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m59HUcZA010269 14, 24 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 12:30:38 -0500 14, 24 -- Received: from [68.142.200.221] by n25.bullet.mail.mud.yahoo.com with NNFMP; 09 Jun 2008 17:30:37 -0000 14, 24 -- Received: from [216.252.122.217] by t9.bullet.mud.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- Received: from [69.147.65.157] by t2.bullet.sp1.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- Received: from [127.0.0.1] by omp405.mail.sp1.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- X-Yahoo-Newman-Property: ymail-3 14, 24 -- X-Yahoo-Newman-Id: 373802.7282.bm-at-omp405.mail.sp1.yahoo.com 14, 24 -- Received: (qmail 52867 invoked by uid 60001); 9 Jun 2008 17:30:38 -0000 14, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 24 -- s=s1024; d=yahoo.com; 14, 24 -- h=Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Messa ge-ID; 14, 24 -- b=MmN8GpdqMPfkJ8e1Y13HohpNQMOpWjzKhvDAKy6iQPq621WBRFjwM2JSPQdvPjBJapl8Vr reH4f6KLJ1+4DJcUkjviL94smRv0c9t6mt6SNrNJt5V8vUxipA3c5HyAzLXJ/0Lm0kVCNuFt 7AvL4Alzh87wcEIvm7CawsSKXwatg=; 14, 24 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Mon, 09 Jun 2008 10:30:38 PDT 14, 24 -- X-Mailer: YahooMailWebService/0.7.199 14, 24 -- Date: Mon, 9 Jun 2008 10:30:38 -0700 (PDT) 14, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 14, 24 -- Subject: 35mm film expiration date 14, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 14, 24 -- MIME-Version: 1.0 14, 24 -- Content-Type: text/plain; charset=us-ascii 14, 24 -- Message-ID: {183151.50499.qm-at-web46101.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 27, 26 -- From TindallR-at-missouri.edu Mon Jun 9 15:16:15 2008 27, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m59KGFnd029686 27, 26 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 15:16:15 -0500 27, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 27, 26 -- Mon, 9 Jun 2008 15:16:15 -0500 27, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 26 -- Content-class: urn:content-classes:message 27, 26 -- MIME-Version: 1.0 27, 26 -- Content-Type: text/plain; 27, 26 -- charset="us-ascii" 27, 26 -- Subject: RE: [Microscopy] 35mm film expiration date 27, 26 -- Date: Mon, 9 Jun 2008 15:16:14 -0500 27, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD76DE-at-UM-XMAIL08.um.umsystem.edu} 27, 26 -- In-Reply-To: {200806091732.m59HW8YI011958-at-ns.microscopy.com} 27, 26 -- X-MS-Has-Attach: 27, 26 -- X-MS-TNEF-Correlator: 27, 26 -- Thread-Topic: [Microscopy] 35mm film expiration date 27, 26 -- Thread-Index: AcjKVr6iO46OZTmvRX2TR2WwXsaY3wAAClSQ 27, 26 -- References: {200806091732.m59HW8YI011958-at-ns.microscopy.com} 27, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 27, 26 -- To: {vapatpxs-at-yahoo.com} 27, 26 -- Cc: {microscopy-at-microscopy.com} 27, 26 -- X-OriginalArrivalTime: 09 Jun 2008 20:16:15.0251 (UTC) FILETIME=[AB325E30:01C8CA6D] 27, 26 -- Content-Transfer-Encoding: 8bit 27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m59KGFnd029686 ==============================End of - Headers==============================
This year at Albuquerque, during Microscopy and Microanalysis 2008, we are holding three special four-day intensive workshops. Each will run from 8:30 AM to 12 noon on Monday, Tuesday, Wednesday, and Thursday, August 4-7.
The workshops are:
-Theory and Techniques of Aberration-Corrected Microscopy
-Basic Principles of Confocal Microscopy
-Nanomaterial Microscopy and Microanalysis: Tools and Preparation
This is a great opportunity for in-depth education from instructors at the top of their fields. Please consider this not only if you are a lab manager or an independent investigator, but also as a great opportunity for students and technicians!
The workshop fee of $850 includes full meeting registration, and you will have every afternoon free to attend the scientific symposia, poster sessions, and exhibits.
For detailed information, please visit:
http://mm2008.microscopy.org/
Click on the “In-Week Workshop” button at the left.
Here, you will see a description of each workshop, with a link to Additional Info at the end of each paragraph.
There is also a poster you can download, from the hypertext “MM2008 Boot Camp Workshops (PDF)” found within the white heading box.
If you are interested, then go back to the "Registration/Forms" button on the left.
The workshops are starting to fill up, so early registration will guarantee you a place. However, if you are not able to make a decision at this time, the fee does not increase after the M&M2008 early-registration deadline (unlike the normal meeting registration).
If you have any questions, just contact me.
Mike Marko marko-at-wadsworth.org For the MSA Education Committee
IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation.
==============================Original Headers============================== 22, 24 -- From marko-at-wadsworth.org Mon Jun 9 16:41:34 2008 22, 24 -- Received: from mailserv.wadsworth.org (mailserv.wadsworth.org [199.184.30.43]) 22, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m59LfXwD013828 22, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 16:41:34 -0500 22, 24 -- X-Wadsworth-MailScanner-Watermark: 1213652488.70366-at-xzfAQZHxlyigfVUAB7Nv6Q 22, 24 -- Received: from [127.0.0.1] (newton12-30.wadsworth.org [172.16.12.30]) 22, 24 -- by mailserv.wadsworth.org (8.13.8+Sun/8.13.8) with ESMTP id m59LfPoj013771 22, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 17:41:28 -0400 (EDT) 22, 24 -- Message-ID: {484DA381.4060905-at-wadsworth.org} 22, 24 -- Date: Mon, 09 Jun 2008 17:41:21 -0400 22, 24 -- From: Michael Marko {marko-at-wadsworth.org} 22, 24 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 22, 24 -- MIME-Version: 1.0 22, 24 -- To: Microscopy-at-microscopy.com 22, 24 -- Subject: Special microscopy courses at M&M2008 22, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 22, 24 -- Content-Transfer-Encoding: 8bit 22, 24 -- X-Wadsworth-MailScanner-Information: Please contact CSS for more information 22, 24 -- X-MailScanner-ID: m59LfPoj013771 22, 24 -- X-Wadsworth-MailScanner: Found to be clean 22, 24 -- X-Wadsworth-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 22, 24 -- score=-4.399, required 5, autolearn=not spam, ALL_TRUSTED -1.80, 22, 24 -- BAYES_00 -2.60) 22, 24 -- X-MailScanner-From: marko-at-wadsworth.org ==============================End of - Headers==============================
Anyone care to comment on the use of d-limonene based clearing agents (e.g. Citri-solv and Citri-solve hybrid) vs. aliphatic hydrocarbon based ones (e.g., Hydrosolv). My use is dewaxing paraffin slides prior to immunocytochemistry. I won't be embedding any tissues since I have that done at a core facility. Thanks for your input. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Does anyone have a turbopump, an Edwards EXT 70, for a Leo 430 sigma SEM they might be willing to part with?.
If anyone can help me in this regard, please contact me offline.
Steve -- Dr. Steven Barlow EM Facility/Biology Dept. San Diego State University 5500 Campanile Drive San Diego CA 92182-4614 phone: (619) 594-4523 fax: (619) 594-5676
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==============================Original Headers============================== 16, 20 -- From gary-at-gaugler.com Mon Jun 9 19:53:50 2008 16, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5A0roKY027326 16, 20 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 19:53:50 -0500 16, 20 -- Message-Id: {200806100053.m5A0roKY027326-at-ns.microscopy.com} 16, 20 -- Received: (qmail 14925 invoked from network); 9 Jun 2008 17:53:50 -0700 16, 20 -- Received: by simscan 1.1.0 ppid: 14919, pid: 14921, t: 0.1351s 16, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 16, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 16, 20 -- by smtp2 with SMTP; 9 Jun 2008 17:53:50 -0700 16, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 20 -- Date: Mon, 09 Jun 2008 17:53:44 -0700 16, 20 -- To: sbarlow-at-sunstroke.sdsu.edu 16, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 16, 20 -- Subject: Re: [Microscopy] need replacement Edwards EXT 70 turbopump..... 16, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 16, 20 -- In-Reply-To: {200806100024.m5A0OeKg015904-at-ns.microscopy.com} 16, 20 -- References: {200806100024.m5A0OeKg015904-at-ns.microscopy.com} 16, 20 -- Mime-Version: 1.0 16, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-312C40DE ==============================End of - Headers==============================
Tom- We switched to the d-limonene solvents for our paraffin work about 4 years ago, in no small part for the lowered toxicity (not to mention the nicer smell). For our embedding protocols, we did have to modify the times slightly, but otherwise, we see no difference. They work very well for dewaxing, and although we don't perform the immunolabellings here as a service, our clients have not found any significant differences. It also made our Environmental Safety people happy when we were not longer giving them gallons of xylenes in our chemical waste pick-ups (we have a paraffin tissue processor). Lee
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-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
We have extended our search for a Director of our Imaging Technology Group, here at the Beckman Institute for Advanced Science and Technology, on the University of Illinois, Urbana-Champaign campus.
Link to announcement: http://www.itg.uiuc.edu/announcements/director.htm
If you are interested please contact:
Beckman Institute Human Resources 1209 Beckman Institute 405 N. Mathews Avenue, MC-251 Urbana, Illinois 61801 Email: jobs-at-beckman.uiuc.edu Phone: 217-244-4474 Fax: 217-333-5441
--------Position Announcement:---------
Director, Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign
The Beckman Institute has a position opening for one (1) Director, Imaging Technology Group (ITG). The ITG's primary mission is to provide state-of-the-art imaging and visualization resources for researchers at the Institute and the University of Illinois. This mission is accomplished through two facilities: a Microscopy Suite, and a Visualization, Media, and Imaging Laboratory. A secondary mission of the ITG is to develop advanced technologies with an emphasis on projects in remote and virtual instrumentation. The ITG Director sets the vision for this group of approximately 25 staff members, writes grants and directs special projects to support that vision, and conducts daily management of the staff.
Duties and Responsibilities: . Supervise the personnel and operations of the Visualization, Media, and Imaging Laboratory (VMIL), Microscopy Suite, ITG Systems Team, ITG Special Projects Team, and ITG Administrative Support. . Develop, manage, and execute new and continuing research projects that utilize ITG facilities and staff, showcase Beckman Institute technologies, promote Beckman faculty, and generally increase the reputation of ITG and Beckman as a leader in the areas of imaging, visualization, research presentation, educational outreach, and multidisciplinary research. . Build collaborations between and participate with faculty members for new projects. Seek external funding for those that become viable. . Work with faculty to leverage ITG projects and facilities as potential outreach components for faculty grants. Author external instrumentation grants to acquire new high-cost instrumentation that supports Beckman research. . Ensure that ITG continues to provide the highest level of support to its users and special services clients. . In coordination with administration and faculty, cultivate and implement strategic planning for the development of ITG's facilities towards the goal of facilitating current, emergent, and future research needs of Beckman's faculty. . Produce creative projects that illustrate and/or contribute to Beckman Institute research (i.e. animations, still images, videos, etc.). . Provide technical direction for software development projects that support the Institute's missions in research and outreach. . Evaluate new hardware and software options for ITG. . Provide budget planning and spending oversight for ITG.
Minimum Qualifications: . A Bachelor's degree. . Significant professional experience in two or more of the following: microscopy, visualization, multimedia production, research computing, interface design. . Three years supervisory experience of full-time professional personnel. . Management of a multi-user facility with a reputation for the highest level of service to its users. . Excellent written and oral communication skills are required.
Preferred Qualifications: . A Master's or Ph.D. degree with a focus in the sciences or other relevant discipline. . Previous experience with successful direction of a high-use multidisciplinary microscopy or imaging facility within a research environment, including staff supervision, long-range planning, new technology evaluation/implementation, and budget planning. . A history of funded research and/or successful grant writing. . Demonstrated ability to constantly follow and accurately evaluate new hardware and software options in the microscopy, computer, graphics, and/or imaging industries. . Research project and educational outreach program development (including conception and technical direction), and external funding acquisition to support those projects. . Experience leading software development teams and projects. . Art direction and/or multimedia production experience. . Demonstrated ability to effectively communicate with staff and users who have diverse backgrounds, including microscopy, visualization, information technology, fine arts, engineering, physical sciences, computer science, and others.
The Director, Imaging Technology Group is a 12-month, 100%-time, benefits-eligible Academic Professional position. All applicants must be eligible to work in the United States and salary is commensurate with qualifications and experience. Applications must be received by July 31, 2008, for full consideration. Applicants may be interviewed prior to the closing date; however, no hiring decision will be made until after that date. The expected start date is as soon as possible following the closing date. Please submit a detailed cover letter explaining your qualifications for the position, resume, and three letters from professional references, preferably via email, to the following:
Beckman Institute Human Resources 1209 Beckman Institute 405 N. Mathews Avenue, MC-251 Urbana, Illinois 61801 Email: jobs-at-beckman.uiuc.edu Phone: 217-244-4474 Fax: 217-333-5441
The University of Illinois is an affirmative action, equal opportunity employer.
-----------------End---------------
Cheers,
Jonathan M. Ekman Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 N. Mathews Avenue Urbana, IL 61801 USA Tel: 217-244-6292 Fax: 217-244-6219
==============================Original Headers============================== 19, 19 -- From jekman-at-uiuc.edu Tue Jun 10 15:50:21 2008 19, 19 -- Received: from expredir5.cites.uiuc.edu (expredir5.cites.uiuc.edu [128.174.5.96]) 19, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5AKoLHE032711 19, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Jun 2008 15:50:21 -0500 19, 19 -- Received: from rollinsitg (rollins.itg.uiuc.edu [130.126.126.232]) 19, 19 -- by expredir5.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id m5AKoLoV019542 19, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 10 Jun 2008 15:50:21 -0500 (CDT) 19, 19 -- From: "Jon Ekman" {jekman-at-uiuc.edu} 19, 19 -- To: {Microscopy-at-microscopy.com} 19, 19 -- Subject: Open Position --Director, Imaging Technology Group 19, 19 -- Date: Tue, 10 Jun 2008 15:50:21 -0500 19, 19 -- Message-ID: {FD35A49813294AEA825F1375969948D6-at-rollinsitg} 19, 19 -- MIME-Version: 1.0 19, 19 -- Content-Type: text/plain; 19, 19 -- charset="us-ascii" 19, 19 -- Content-Transfer-Encoding: 7bit 19, 19 -- X-Mailer: Microsoft Office Outlook 11 19, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 19, 19 -- thread-index: AcjLO5kfu1E3q3cUSqi/BLNge2+/CA== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sonali_mookerjee-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sonali_mookerjee-at-yahoo.com Name: Sonali
Organization: Michigan State University
Title-Subject: [Filtered] Apple shoot apex with SEM
Question: I am studying the developmental stages of apple inflorescence and I am trying to get an image of the developing shoot apex transitioning from vegetative to floral meristem and then developing flower primordia. However, I am unable to dissect out the apex to be able to view the meristem under SEM. It either had at least a layer of primordia hiding the apex, or when I tried to dissect it further, I am damaging the apex completely. I am using a dissecting microscope and very fine forceps and needle for dissection.
Does anyone have any suggestion on how I can do a better dissection?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ek59-at-drexel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ek59-at-drexel.edu Name: Ed Keough
Organization: Drexel University, BMES
Title-Subject: [Filtered] TEM SEM Image automated particle segmentation and quanititative analsysis
Question: Hello. I am interested in quantifying count and sizes of particles in TEM (or SEM ) images. My experience is doing this with cell-based fluorescent images in imagej or a few other platform-specific software programs that really require good contrast in images to identify and segment objects. The objects in these images are very "grainy" for lack of a better word; you can not use a strict contrast threshold. I had limited success with these programs with image enhancing in imagej (denoising, anisotropic diffusion, etc.).
I see through the archives that many people just trace lines by hand or use imagej or photoshop plug-ins. Is anyone aware of specific plug-ins or tools that may be useful, or a good method to extract the objects and get them to be contrast based? I'm interested in making it as automated as possible and I'm open to any software.
The only way I have been able to reliably dissect out the SAM (of Arabidopsis in my case) at any stage is by using a sapphire dissecting knife. For Arabidopsis, I pin the hypocotyl into a groove cut into very firm polythene foam (used for refrigerator insulation), using the tiniest insect pinning needles, with the SAM pointing directly up. Keeping it reasonably wet but not flooded, I carefully cut away the leaves and primordia with the sapphire knife. The good thing about sapphire knives (the lance-style ones are best, I find) is that they are transparent, so you can see what you're doing all the time. They're available from World Precision Instruments - no commercial interest, etc.
Then you can image the SAM immediately using a water dipping objective on the confocal - or process for SEM.
good luck!
cheers, Rosemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
On 11/6/08 12:13 PM, "sonali_mookerjee-at-yahoo.com" {sonali_mookerjee-at-yahoo.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sonali_mookerjee-at-yahoo.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: sonali_mookerjee-at-yahoo.com } Name: Sonali } } Organization: Michigan State University } } Title-Subject: [Filtered] Apple shoot apex with SEM } } Question: I am studying the developmental stages of apple } inflorescence and I am trying to get an image of the developing shoot } apex transitioning from vegetative to floral meristem and then } developing flower primordia. } However, I am unable to dissect out the apex to be able to view the } meristem under SEM. It either had at least a layer of primordia } hiding the apex, or when I tried to dissect it further, I am damaging } the apex completely. I am using a dissecting microscope and very } fine forceps and needle for dissection. } } Does anyone have any suggestion on how I can do a better dissection? } } Thanks } } Login Host: 76.122.130.227 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Jun 10 21:05:32 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m5B25VNX022172 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jun 2008 21:05:32 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c474e3592542-at-[206.69.208.22]} } 8, 11 -- Date: Tue, 10 Jun 2008 21:05:30 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: sonali_mookerjee-at-yahoo.com (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Apple shoot apex with SEM } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 23 -- From prvs=Rosemary.White=041c5c4c8-at-csiro.au Tue Jun 10 21:37:06 2008 11, 23 -- Received: from act-MTAout4.csiro.au (act-MTAout4.csiro.au [150.229.7.41]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5B2b44b015687 11, 23 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jun 2008 21:37:05 -0500 11, 23 -- X-IronPort-AV: E=Sophos;i="4.27,621,1204462800"; 11, 23 -- d="scan'208";a="932006" 11, 23 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 11, 23 -- by act-ironport-int.csiro.au with ESMTP; 11 Jun 2008 12:37:03 +1000 11, 23 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 11, 23 -- Wed, 11 Jun 2008 12:37:03 +1000 11, 23 -- User-Agent: Microsoft-Entourage/11.0.0.040405 11, 23 -- Date: Wed, 11 Jun 2008 12:43:56 +1000 11, 23 -- Subject: Re: [Microscopy] viaWWW: Apple shoot apex with SEM 11, 23 -- From: Rosemary White {rosemary.white-at-csiro.au} 11, 23 -- To: {sonali_mookerjee-at-yahoo.com} 11, 23 -- CC: {microscopy-at-microscopy.com} 11, 23 -- Message-ID: {C475790C.BF62%rosemary.white-at-csiro.au} 11, 23 -- In-Reply-To: {200806110213.m5B2DXkE008581-at-ns.microscopy.com} 11, 23 -- Mime-version: 1.0 11, 23 -- Content-type: text/plain; 11, 23 -- charset="US-ASCII" 11, 23 -- Content-transfer-encoding: 7bit 11, 23 -- X-OriginalArrivalTime: 11 Jun 2008 02:37:03.0820 (UTC) FILETIME=[086B10C0:01C8CB6C] ==============================End of - Headers==============================
Dear colleagues We would like to bring to you attention 2 planned workshops on Piezoresponse Force Microscopy and Nanoscale Electromechanics, to be held in US and Europe.
- The 3d International workshop on PFM is planned for September 23-25 in ORNL, Oak Ridge, TN http://cnms.ornl.gov/upcoming_events/events.shtm http://cnms.ornl.gov/workshops/2008/CNMS_PFM_Workshop_2008.pdf
- The 4th international workshop on PFM is planned for February 2009, Aveiro, Portugal ftp://ftp.ua.pt/incoming/4th_PFM_workshop/4thWorkshop_Aveiro.pdf
These workshops follow the Fall 2007 workshop at ORNL and May 2008 workshop in EPFL, Lausanne, Switzerland, featuring ~40 and 60 attendees respectively. The detailed information on the scope, program, and contact information is summarized on-line, and can be obtained from the organizers (sergei2-at-ornl.gov and kholkin-at-cv.ua.pt).
In addition to tutorial and invited speaker program, we plan to include several sessions on recent advances in PFM technique and applications to be presented by the attendees. The program tentatively includes the presentations by SPM manufacturers providing the information on state of the art of commercially-available methods.
Yours Sergei Kalinin and Andrei Kholkin
Dear colleagues
We are bringing to your attention the 3d International Workshop "Piezoresponse Force Microscopy: Instrumentation, Techniques, and Applications" to be held at Oak Ridge, TN on September 22-25, 2008, in conjunction with the CNMS User Meeting (http://cnms.ornl.gov/). The workshop focuses on electromechanical coupling in inorganic and macromolecular materials and biological systems and will feature invited tutorials on emergent phenomena in nanoscale ferroelectrics and ferroelectric surfaces, polarization-mediated surface phenomena in polar materials, and piezoelectricity and electrophysiology of biosystems. The central theme of the workshop - Piezoresponse Force Microscopy - will be discussed in a series of tutorials by S.V. Kalinin (ORNL) and A. Gruverman (UNL), that will introduce basic principles of PFM operation, relevant instrumental aspects, and recent advances in PFM imaging of switching ferroelectric films and nanostructures, biological materials and macromolecular systems, and PFM in liquid and ultra high vacuum environment. The tutorials will be complemented by a poster session and manufacturer presentations. The workshop will also feature a series of presentation of practitioners of Switching Spectroscopy PFM, demonstrating recent advances in application of this technique to ferroelectric and ferroelastic domain walls, capacitors, multicomponent materials, and ferroelectric multilayers.
The 2.5 day workshop will be followed by 1.5 days of experimental demonstrations of PFM, Switching Spectroscopy PFM, and band-excitation PFM by CNMS staff members, held in parallel with the CNMS user meeting. The participants are welcome to bring their own samples. The workshop abstract and outline are available on line at *http://cnms.ornl.gov/workshops/2008/CNMS_PFM_Workshop_2008.pdf*. The registration information will be available shortly at www.cnms.ornl.gov
The invited speakers include:
* S. Streiffer, Argonne National Lab, "Phase transitions, domain structure & dynamics, and surface chemistry in ferroelectrics studied by x-rays"
· A. Kholkin, U. Aveiro, Portugal "Nanoscale imaging and polarization dynamics in relaxor ferroelectrics"
* J. Junquera, University of Cantabria, Spain, “First-principles modeling of ferroelectric surfaces and nanostructures” * W. Brownell, Baylor College of Medicine, “Nanoscale electromechanics in biological systems”
Due to the space limit, please contact workshop organizers [sergei2-at-ornl.gov] by August 31 if you are interested in attending.
Yours
Sergei V. Kalinin and Arthur P. Baddorf
==============================Original Headers============================== 19, 25 -- From sergei2-at-ornl.gov Wed Jun 11 13:23:10 2008 19, 25 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5BIN94c000875 19, 25 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2008 13:23:10 -0500 19, 25 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 19, 25 -- by emroute1.ornl.gov (PMDF V6.3-x14 #31501) 19, 25 -- with ESMTP id {0K2B00E0T9QG93-at-emroute1.ornl.gov} for 19, 25 -- microscopy-at-microscopy.com; Wed, 11 Jun 2008 14:23:05 -0400 (EDT) 19, 25 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 19, 25 -- (PMDF V6.3-x14 #31501) id {0K2B00E019QGHW-at-emroute1.ornl.gov} ; Wed, 19, 25 -- 11 Jun 2008 14:23:04 -0400 (EDT) 19, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 19, 25 -- by emroute1.ornl.gov (PMDF V6.3-x14 #31501) 19, 25 -- with ESMTP id {0K2B00H4M9QGDJ-at-emroute1.ornl.gov} ; Wed, 19, 25 -- 11 Jun 2008 14:23:04 -0400 (EDT) 19, 25 -- Date: Wed, 11 Jun 2008 14:23:04 -0400 19, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 19, 25 -- Subject: 3d and 4th Workshops on Piezoresponse Force Microscopy, 19, 25 -- Nanoferroelectrics, and Nanoscale Electromechanics 19, 25 -- To: microscopy-at-microscopy.com, Andrei Kholkin {kholkin-at-ua.pt} 19, 25 -- Message-id: {48501808.30106-at-ornl.gov} 19, 25 -- MIME-version: 1.0 19, 25 -- Content-type: text/plain; charset=windows-1252; format=flowed 19, 25 -- Content-transfer-encoding: 8BIT 19, 25 -- User-Agent: Thunderbird 1.5.0.14 (Windows/20071210) ==============================End of - Headers==============================
Can y'all lend some expert advice as how to optimize a protocol for post-embed labelling on brains?
I have a user who has been doing post-embed labelling on brain tissue which has been embedded in Araldite with the traditional fixation and embedding protocols (Karnovsky's, OsO4, ETOH, PO and Araldite). She has to etch her sections and other damaging things which limits ultrastructural beauty and labelling prettiness. I'm suggesting LR White but I want to know what the experts are doing.
So please, rack your brains to help her with her brains.
Thanks in advance,
Paula :-)
Paula Sicurello VA Hospital San Diego Microscope Facility room B141 3350 La Jolla Village Drive San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 10, 24 -- From vapatpxs-at-yahoo.com Wed Jun 11 17:28:07 2008 10, 24 -- Received: from n61.bullet.mail.sp1.yahoo.com (n61.bullet.mail.sp1.yahoo.com [98.136.44.37]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5BMS6ad022220 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2008 17:28:06 -0500 10, 24 -- Received: from [216.252.122.218] by n61.bullet.mail.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 10, 24 -- Received: from [69.147.65.153] by t3.bullet.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 10, 24 -- Received: from [127.0.0.1] by omp401.mail.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 10, 24 -- X-Yahoo-Newman-Property: ymail-3 10, 24 -- X-Yahoo-Newman-Id: 337875.13899.bm-at-omp401.mail.sp1.yahoo.com 10, 24 -- Received: (qmail 36285 invoked by uid 60001); 11 Jun 2008 22:28:06 -0000 10, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 24 -- s=s1024; d=yahoo.com; 10, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 24 -- b=gjkWtfH8purmW2pxWkwMgpChyTLQx9mouiS2NDwXadtclel5TgQiyqrR1F9x3oA7tIGZ9W4M5NJd06dmNhk5OPnKJJhPvxnc9ct8SfHgUO7hFh0LGyxZXt+eWInGSsg/4+/ArQWEiRSXyiOmKO4D+sEiUgmdm7dRwPhQdvMM1Vk=; 10, 24 -- Received: from [132.239.85.200] by web46112.mail.sp1.yahoo.com via HTTP; Wed, 11 Jun 2008 15:28:05 PDT 10, 24 -- X-Mailer: YahooMailWebService/0.7.199 10, 24 -- Date: Wed, 11 Jun 2008 15:28:05 -0700 (PDT) 10, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 24 -- Reply-To: vapatpxs-at-yahoo.com 10, 24 -- Subject: ImmunoEM on brain 10, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=us-ascii 10, 24 -- Message-ID: {128009.36045.qm-at-web46112.mail.sp1.yahoo.com} ==============================End of - Headers==============================
The specimen as you describe it is probably not the best situation to start with, although there have been reports of successful immuno labelling on tissue that was OsO4 fixed and epoxy embedded. So if that is what you get, you might have to give it a go. LR-White embedding means you have to go through another run of specimen preparation. While you are at that...is there no way you could consider a pre-embedding approach? If you let me have more details, I will be happy to try to help. You may also want to talk to Hong Yi at Emory University, Atlanta GA, who has vast experience in this field.
Jan Leunissen
Aurion - President Costerweg 5 Honorary Research Fellow 6702 AA Wageningen Otago School of Medical Sciences The Netherlands Dunedin phone 31-317-497676 New Zealand fax 31-317-415955 www.aurion.nl
==============================Original Headers============================== 6, 21 -- From leunissen-at-aurion.nl Wed Jun 11 18:06:43 2008 6, 21 -- Received: from fep03.xtra.co.nz (fep03.xtra.co.nz [210.54.141.243]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5BN6gro003656 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2008 18:06:42 -0500 6, 21 -- Received: from mta06.xtra.co.nz ([172.23.11.61]) by fep03.xtra.co.nz 6, 21 -- with ESMTP 6, 21 -- id {20080611230640.RXNR29448.fep03.xtra.co.nz-at-mta06.xtra.co.nz} ; 6, 21 -- Thu, 12 Jun 2008 11:06:40 +1200 6, 21 -- Received: from [192.168.1.50] (really [219.89.62.35]) by mta06.xtra.co.nz 6, 21 -- with ESMTP 6, 21 -- id {20080611230640.ZOIB4312.mta06.xtra.co.nz-at-[192.168.1.50]} ; 6, 21 -- Thu, 12 Jun 2008 11:06:40 +1200 6, 21 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 6, 21 -- Message-Id: {C280BAF7-7337-49CA-BF3F-4A7FC2DA8222-at-aurion.nl} 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- From: Jan Leunissen {leunissen-at-aurion.nl} 6, 21 -- Subject: Re: [Microscopy] ImmunoEM on brain 6, 21 -- Date: Thu, 12 Jun 2008 11:06:39 +1200 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cogswell-at-nbnet.nb.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cogswell-at-nbnet.nb.ca Name: Steven Cogswell
Organization: UNB Microscopy
Title-Subject: [Filtered] Recycling for Platinum apertures
Question: Hey microscopy folks;
We just decommissioned a couple of old TEM units, and thus I'm left with a surplus of platinum apertures. It's a lot of apertures, but the problem is it's not a lot of mass: about 4 grams in total. I contacted a refiner and they want at least 30 g.
Does anyone know a decent refiner/whatever that will take small quantities?
Thanks for your time, Steven Cogswell UNB Microscopy and Microanalysis
Using Epon may not be far off the mark when it comes to brain immunocytochemistry. There is a group in Norway who have been successfully using it for a while (O.P Ottersen). More recently they switched to Durcupan (Gundersen et al J Cerebral Blood Flow & Metabolism (2001) 21, 41–51; http://www.nature.com/jcbfm/journal/v21/n1/abs/9591036a.html;jsessionid=178E36E3B37B167B1C682EA6ED331497).
Although other resins may be more favorable, they will bring other problems. For example, LR White is a very good solvent that may take away brain lipids. You may also encounter polymerization problems too if you are not using this resin routinely. There will also be a different contrast that your collaborator may not appreciate (low contrast myelin)
Of course, the final answer to your question is to try one or two different options and see which works best for your antigen and antibody combination.
Have you considered cryosections of cryoprotected material. This would be an excellent approach for antigens that are not washed away during aldehyde fixation. The only disadvantage could be that the myelin will not have the black appearance usual for tissue treated with osmium. Interestingly, someone just told me that adding uranyl acetate to the pick-up solution will create the black (artificial) color if you really want it.
Regards,
Paul.
Paul Webster, Ph.D. House Ear Institute 2100 W. 3rd ST. Los Angeles. CA 90057
-----Original Message----- X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] Sent: Wed 6/11/2008 3:33 PM To: Webster, Paul
Hello Listers,
Can y'all lend some expert advice as how to optimize a protocol for post-embed labelling on brains?
I have a user who has been doing post-embed labelling on brain tissue which has been embedded in Araldite with the traditional fixation and embedding protocols (Karnovsky's, OsO4, ETOH, PO and Araldite). She has to etch her sections and other damaging things which limits ultrastructural beauty and labelling prettiness. I'm suggesting LR White but I want to know what the experts are doing.
So please, rack your brains to help her with her brains.
Thanks in advance,
Paula :-)
Paula Sicurello VA Hospital San Diego Microscope Facility room B141 3350 La Jolla Village Drive San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 10, 24 -- From vapatpxs-at-yahoo.com Wed Jun 11 17:28:07 2008 10, 24 -- Received: from n61.bullet.mail.sp1.yahoo.com (n61.bullet.mail.sp1.yahoo.com [98.136.44.37]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5BMS6ad022220 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2008 17:28:06 -0500 10, 24 -- Received: from [216.252.122.218] by n61.bullet.mail.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 10, 24 -- Received: from [69.147.65.153] by t3.bullet.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 10, 24 -- Received: from [127.0.0.1] by omp401.mail.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 10, 24 -- X-Yahoo-Newman-Property: ymail-3 10, 24 -- X-Yahoo-Newman-Id: 337875.13899.bm-at-omp401.mail.sp1.yahoo.com 10, 24 -- Received: (qmail 36285 invoked by uid 60001); 11 Jun 2008 22:28:06 -0000 10, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 24 -- s=s1024; d=yahoo.com; 10, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 24 -- b=gjkWtfH8purmW2pxWkwMgpChyTLQx9mouiS2NDwXadtclel5TgQiyqrR1F9x3oA7tIGZ9W4M5NJd06dmNhk5OPnKJJhPvxnc9ct8SfHgUO7hFh0LGyxZXt+eWInGSsg/4+/ArQWEiRSXyiOmKO4D+sEiUgmdm7dRwPhQdvMM1Vk=; 10, 24 -- Received: from [132.239.85.200] by web46112.mail.sp1.yahoo.com via HTTP; Wed, 11 Jun 2008 15:28:05 PDT 10, 24 -- X-Mailer: YahooMailWebService/0.7.199 10, 24 -- Date: Wed, 11 Jun 2008 15:28:05 -0700 (PDT) 10, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 24 -- Reply-To: vapatpxs-at-yahoo.com 10, 24 -- Subject: ImmunoEM on brain 10, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=us-ascii 10, 24 -- Message-ID: {128009.36045.qm-at-web46112.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 28, 21 -- From PWebster-at-hei.org Wed Jun 11 21:00:42 2008 28, 21 -- Received: from hi0sml1.hei.org (House-Ear-Institute-1114861.cust-rtr.pacbell.net [71.136.31.242]) 28, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5C20gWM003573 28, 21 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2008 21:00:42 -0500 28, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 28, 21 -- Content-class: urn:content-classes:message 28, 21 -- MIME-Version: 1.0 28, 21 -- Content-Type: text/plain; 28, 21 -- charset="iso-8859-1" 28, 21 -- Subject: RE: [Microscopy] ImmunoEM on brain 28, 21 -- Date: Wed, 11 Jun 2008 18:55:50 -0700 28, 21 -- Message-ID: {87449E4A2B01DA47B29424CE5D6E0F830814C998-at-hi0sml1.hei.org} 28, 21 -- X-MS-Has-Attach: 28, 21 -- X-MS-TNEF-Correlator: 28, 21 -- Thread-Topic: [Microscopy] ImmunoEM on brain 28, 21 -- Thread-Index: AcjMEyCgS+Pa2aG0Q5Ku2FKciE1jeQAHFATj 28, 21 -- References: {200806112233.m5BMX9rS028129-at-ns.microscopy.com} 28, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 28, 21 -- To: {vapatpxs-at-yahoo.com} , {microscopy-at-microscopy.com} 28, 21 -- Content-Transfer-Encoding: 8bit 28, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5C20gWM003573 ==============================End of - Headers==============================
Early last year at the Berkeley Cryotechniques and Immunogold Labeling Workshop, one of our participants insisted to give her a best protocol for immunogold labeling. To her disappointment, I had to say that there is no universal one-fits-all protocol for immunogold labeling. It all depends on what is being labeled and what questions need to be answered with the labeling.
There are many approaches for immunogold labeling in brain tissue. Post-embedding labeling on conventionally fixed and Epoxy resin embedded material is one of them, however this method is only suitable for a small category of antigens, among which is a group of amino acids used by the brain as neurotransmitters. The antisera against these small amino acids usually are raised by immunizing rabbits with the respective amino acids that are conjugated to a carrier protein (e.g. BSA) using glutaraldehyde. Therefore, the preservation of the epitopes of these amino acids in brain tissue is not incompatible with glutaraldehyde fixation, and this is one of the reasons conventional embedding is fine for the labeling of these amino acids. The labeling described in the paper Paul listed in his reply is an example of this type of labeling.
However, the majority of immunogold labeling in brain tissue is for antigens that are far more “vulnerable”. Although using milder aldehyde fixative, omitting OsO4, and switching to LR White resin may minimize the “damage” to the antigens, I do not recommend this approach for brain tissue. In brain tissue, the identification of neuronal structure relies heavily on the integrity of membrane ultrastructure. In addition, many labelings are for membrane bound proteins such as receptor proteins, channel proteins, or transporter proteins. In its protocol, LR White embedding is usually accompanied by the omission of OsO4 fixation which means lipid is not stabilized so more likely to be extracted or “melted” during dehydration and thermo polymerization. For this reason, LR White embedded brain tissue will not give the adequate membrane ultrastructure required. If your user has a particular reason to insist on post-embedding labeling, I would recommend high pressure freezing, cryo-substitution, and embedding in Lowicryl. There have been many publications using this approach for brain tissue showing good membrane integrity.
In general, the far more common approach, or I should say the approach people usually start with for immuno labeling in brain tissue is pre-embedding labeling. One aspect of immunolabeling in brain tissue is to survey the distribution of the protein in a large and structurally heterogeneous area before dissecting out the right area for EM analysis. For this reason, pre-embedding labeling offers a major advantage because the labeling can be seen with LM in large vibratome sections that cross any region of the brain. Pre- embedding immuno labeling has other benefits as well which we can discuss later.
As Paul mentioned your user might want to try one of two different approaches, however, he/she should not try randomly. Again, find out first what is being labeled and what questions need to be answered with this labeling.
Back to Araldite embedded material, if the etching is done right, he/she should be able to get beautiful ultrastructure. I would be glad to talk to him/her off-line if you like.
Hope this helps and thank you.
Hong ============================= Hong Yi Technical Director Robert Apkarian Integrated Electron Microscopy Core Room E108 Cherry L. Emerson Hall 1515 Dickey Drive, NE Emory University Atlanta, GA 30322
Tel: (404) 712-8491 Fax: (404) 727-7760 hyi-at-emory.edu Video conference via iChat: hongyiga-at-mac.com
==============================Original Headers============================== 12, 23 -- From hyi-at-emory.edu Thu Jun 12 06:56:43 2008 12, 23 -- Received: from QMTA04.emeryville.ca.mail.comcast.net (qmta04.emeryville.ca.mail.comcast.net [76.96.30.40]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5CBug54031662 12, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 06:56:42 -0500 12, 23 -- Received: from OMTA11.emeryville.ca.mail.comcast.net ([76.96.30.36]) 12, 23 -- by QMTA04.emeryville.ca.mail.comcast.net with comcast 12, 23 -- id cyuu1Z0030mlR8UA403300; Thu, 12 Jun 2008 11:56:42 +0000 12, 23 -- Received: from [71.236.25.99] ([71.236.25.99]) 12, 23 -- by OMTA11.emeryville.ca.mail.comcast.net with comcast 12, 23 -- id czwf1Z00128HRMB8XzwfnH; Thu, 12 Jun 2008 11:56:39 +0000 12, 23 -- X-Authority-Analysis: v=1.0 c=1 a=uoukCOFTqgbgLFIkTnMA:9 12, 23 -- a=Ab0gvdy4FQkjwfDI3f8A:7 a=vw3vZxrG_AvGjr9EUx-xEYlrXOAA:4 a=gUe8_R_abCcA:10 12, 23 -- a=tt7VLuxcIEYA:10 a=98jSFH7WqmUA:10 a=9OHTkwyHC8cA:10 12, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 12, 23 -- Message-Id: {F1082B08-4FF2-46A8-BC75-F8DF73E84012-at-emory.edu} 12, 23 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; format=flowed 12, 23 -- To: microscopy-at-microscopy.com 12, 23 -- From: Hong Yi {hyi-at-emory.edu} 12, 23 -- Subject: Re: ImmunoEM on brain 12, 23 -- Date: Thu, 12 Jun 2008 07:55:36 -0400 12, 23 -- X-Mailer: Apple Mail (2.753) 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5CBug54031662 ==============================End of - Headers==============================
It all depends on the antigen you are looking for. That said, it is unlikely that traditional EM processing will allow you to find what you are looking for. Before proceeding I suggest a literature search to see how others have localized this antigen at the EM level. This could save someone a lot of time and trouble.
Geoff
vapatpxs-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Listers, } } Can y'all lend some expert advice as how to optimize a protocol for post-embed labelling on brains? } } I have a user who has been doing post-embed labelling on brain tissue which has been embedded in Araldite with the traditional fixation and embedding protocols (Karnovsky's, OsO4, ETOH, PO and Araldite). She has to etch her sections and other damaging things which limits ultrastructural beauty and labelling prettiness. I'm suggesting LR White but I want to know what the experts are doing. } } So please, rack your brains to help her with her brains. } } Thanks in advance, } } Paula :-) } } Paula Sicurello } VA Hospital San Diego } Microscope Facility room B141 } 3350 La Jolla Village Drive } San Diego, CA 92161 } 858-552-8585 x2397 } } } } } } ==============================Original Headers============================== } 10, 24 -- From vapatpxs-at-yahoo.com Wed Jun 11 17:28:07 2008 } 10, 24 -- Received: from n61.bullet.mail.sp1.yahoo.com (n61.bullet.mail.sp1.yahoo.com [98.136.44.37]) } 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5BMS6ad022220 } 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jun 2008 17:28:06 -0500 } 10, 24 -- Received: from [216.252.122.218] by n61.bullet.mail.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 } 10, 24 -- Received: from [69.147.65.153] by t3.bullet.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 } 10, 24 -- Received: from [127.0.0.1] by omp401.mail.sp1.yahoo.com with NNFMP; 11 Jun 2008 22:28:06 -0000 } 10, 24 -- X-Yahoo-Newman-Property: ymail-3 } 10, 24 -- X-Yahoo-Newman-Id: 337875.13899.bm-at-omp401.mail.sp1.yahoo.com } 10, 24 -- Received: (qmail 36285 invoked by uid 60001); 11 Jun 2008 22:28:06 -0000 } 10, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 10, 24 -- s=s1024; d=yahoo.com; } 10, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; } 10, 24 -- b=gjkWtfH8purmW2pxWkwMgpChyTLQx9mouiS2NDwXadtclel5TgQiyqrR1F9x3oA7tIGZ9W4M5NJd06dmNhk5OPnKJJhPvxnc9ct8SfHgUO7hFh0LGyxZXt+eWInGSsg/4+/ArQWEiRSXyiOmKO4D+sEiUgmdm7dRwPhQdvMM1Vk=; } 10, 24 -- Received: from [132.239.85.200] by web46112.mail.sp1.yahoo.com via HTTP; Wed, 11 Jun 2008 15:28:05 PDT } 10, 24 -- X-Mailer: YahooMailWebService/0.7.199 } 10, 24 -- Date: Wed, 11 Jun 2008 15:28:05 -0700 (PDT) } 10, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} } 10, 24 -- Reply-To: vapatpxs-at-yahoo.com } 10, 24 -- Subject: ImmunoEM on brain } 10, 24 -- To: MSA BB {Microscopy-at-microscopy.com} } 10, 24 -- MIME-Version: 1.0 } 10, 24 -- Content-Type: text/plain; charset=us-ascii } 10, 24 -- Message-ID: {128009.36045.qm-at-web46112.mail.sp1.yahoo.com} } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 28 -- From mcauliff-at-umdnj.edu Thu Jun 12 09:41:58 2008 8, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5CEfwgf026495 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 09:41:58 -0500 8, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 8, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id BD5B9A7B79 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 10:41:57 -0400 (EDT) 8, 28 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 8, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id D61F5A7B67 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 10:41:56 -0400 (EDT) 8, 28 -- Received: from ([10.32.15.171]) 8, 28 -- by imail.umdnj.edu with ESMTP id 5502050.18014860; 8, 28 -- Thu, 12 Jun 2008 10:41:38 -0400 8, 28 -- MIME-version: 1.0 8, 28 -- Content-transfer-encoding: 7BIT 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 8, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 8, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0K2C006I8U5DMO10-at-umduwc02.umdnj.edu} for 8, 28 -- microscopy-at-microscopy.com; Thu, 12 Jun 2008 10:41:38 -0400 (EDT) 8, 28 -- Message-id: {485135CC.7070303-at-umdnj.edu} 8, 28 -- Date: Thu, 12 Jun 2008 10:42:21 -0400 8, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 28 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 8, 28 -- To: vapatpxs-at-yahoo.com, microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] ImmunoEM on brain 8, 28 -- References: {200806112229.m5BMTVFd023799-at-ns.microscopy.com} 8, 28 -- In-reply-to: {200806112229.m5BMTVFd023799-at-ns.microscopy.com} ==============================End of - Headers==============================
Dear Steve, You might try Abe Dayani at Refining Systems Inc. (www.refiningsystems.com) I know he will take in precious metals and give you credit for your next sputter target. Regards,
-----Original Message----- X-from: cogswell-at-nbnet.nb.ca [mailto:cogswell-at-nbnet.nb.ca] Sent: June 11, 2008 4:22 PM To: maryflet-at-interchange.ubc.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cogswell-at-nbnet.nb.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cogswell-at-nbnet.nb.ca Name: Steven Cogswell
Organization: UNB Microscopy
Title-Subject: [Filtered] Recycling for Platinum apertures
Question: Hey microscopy folks;
We just decommissioned a couple of old TEM units, and thus I'm left with a surplus of platinum apertures. It's a lot of apertures, but the problem is it's not a lot of mass: about 4 grams in total. I contacted a refiner and they want at least 30 g.
Does anyone know a decent refiner/whatever that will take small quantities?
Thanks for your time, Steven Cogswell UNB Microscopy and Microanalysis
I'm looking for a C-mount color camera for under $2000 US for routine transmitted light still images(brightfield/DIC/phase). I'll not be using it for fluorescence, so I don't need something very sensitive. I've found some under-$2k models that seemingly fit the bill from the following manufacturers and was wondering if people can comment on these or suggest others:
Pixera (Professional PIVCS10132) Lumenera (Infinity1-3) Pixelink (PL-A623-C and PL-B774-U)
Also, what are people's thoughts on CMOS vs. CCD? The one CMOS camera we've tried (not named above) seemed to be more noisy even under high-light conditions (low gain) than CCD and had trouble with white-balance/color fidelity.
Thanks, Esteban
-- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Core Facility University of Missouri 120 Bond Life Sciences Center Columbia, MO 65211
http://www.biotech.missouri.edu/mcc
(573)882-4895 (573)884-9676 fax
==============================Original Headers============================== 2, 23 -- From fernandezg-at-missouri.edu Thu Jun 12 16:02:15 2008 2, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5CL2Fwj019654 2, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 16:02:15 -0500 2, 23 -- Received: from UM-XMAIL03.um.umsystem.edu ([209.106.228.3]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 2, 23 -- Thu, 12 Jun 2008 16:02:15 -0500 2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 23 -- Content-class: urn:content-classes:message 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Type: text/plain; 2, 23 -- charset="iso-8859-1" 2, 23 -- Subject: LM: Opinions on color cameras for brightfield; CMOS vs. CCD 2, 23 -- Date: Thu, 12 Jun 2008 16:02:14 -0500 2, 23 -- Message-ID: {002C76CE90154E4EAD095153F371D71D043EFBFF-at-UM-XMAIL03.um.umsystem.edu} 2, 23 -- X-MS-Has-Attach: 2, 23 -- X-MS-TNEF-Correlator: 2, 23 -- Thread-Topic: Opinions on color cameras for brightfield; CMOS vs. CCD 2, 23 -- thread-index: AcjMyc8hV8BxLSvOTsy9aW6sVEgjQA== 2, 23 -- From: "Fernandez, Gerardo E." {fernandezg-at-missouri.edu} 2, 23 -- To: {microscopy-at-microscopy.com} 2, 23 -- X-OriginalArrivalTime: 12 Jun 2008 21:02:15.0012 (UTC) FILETIME=[9761BA40:01C8CCCF] 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5CL2Fwj019654 ==============================End of - Headers==============================
This time I have a real perplexing problem for all you faithful listers.
I have a binoc head for my UltracutS which appears to have some of the optics, for lack of a better word, frosted. It appears to be inside the head itself because I can see it through both eye holes, eye pieces removed, and by looking up through the bottom of the head as well.
I can't figure out how to open it up, but then that might make it worse.
Has anybody heard of this before? It's irritating because the ultramicrotome is in great shape but it's like looking through frosted glass. So I can't see the water reflection in the boat and I can't see well into the blocks during trimming.
Any suggestions (other than buy a new one) are gratefully accepted.
Thanks,
Paula :-)
Paula Sicurello VA Hospital San Diego Microscope Facility room B141 3350 La Jolla Village Drive San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 12, 24 -- From vapatpxs-at-yahoo.com Thu Jun 12 16:03:02 2008 12, 24 -- Received: from n58.bullet.mail.sp1.yahoo.com (n58.bullet.mail.sp1.yahoo.com [98.136.44.46]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5CL32I9020729 12, 24 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 16:03:02 -0500 12, 24 -- Received: from [216.252.122.219] by n58.bullet.mail.sp1.yahoo.com with NNFMP; 12 Jun 2008 21:03:02 -0000 12, 24 -- Received: from [69.147.65.169] by t4.bullet.sp1.yahoo.com with NNFMP; 12 Jun 2008 21:03:02 -0000 12, 24 -- Received: from [127.0.0.1] by omp504.mail.sp1.yahoo.com with NNFMP; 12 Jun 2008 21:03:02 -0000 12, 24 -- X-Yahoo-Newman-Property: ymail-3 12, 24 -- X-Yahoo-Newman-Id: 27976.89449.bm-at-omp504.mail.sp1.yahoo.com 12, 24 -- Received: (qmail 68970 invoked by uid 60001); 12 Jun 2008 21:03:01 -0000 12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 24 -- s=s1024; d=yahoo.com; 12, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 12, 24 -- b=ADeSwkLO5+6o4ZZk9+6jb8p/kJN7m/yUc8n3PcBWFjWDzKzzzNXujgeAV1HzRsGcYIQnM+i/aY3bYN4mV7NXs4y3HPbUSjOXnANVaAp999vOpSIl6HE4xkSKCBEaE70crHnQZ88kMIMgj6KETZO567apaRPqQj6+NMkuywEHUgQ=; 12, 24 -- Received: from [132.239.85.200] by web46102.mail.sp1.yahoo.com via HTTP; Thu, 12 Jun 2008 14:03:01 PDT 12, 24 -- X-Mailer: YahooMailWebService/0.7.199 12, 24 -- Date: Thu, 12 Jun 2008 14:03:01 -0700 (PDT) 12, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 12, 24 -- Reply-To: vapatpxs-at-yahoo.com 12, 24 -- Subject: Leica StereoZoom 6 12, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 12, 24 -- MIME-Version: 1.0 12, 24 -- Content-Type: text/plain; charset=us-ascii 12, 24 -- Message-ID: {785654.68623.qm-at-web46102.mail.sp1.yahoo.com} ==============================End of - Headers==============================
After a long journey of not adding a digital image acquisition system to my otherwise incredibly useful SEM, the loss of Polaroid forces the issue.
Does anyone have opinions, good/bad/indifferent, for some of the packages out there. I would be interested in satisfaction with sales, support, software interfaces, passive vs active, etc.
Thanks!
Alan Stone ASTON
==============================Original Headers============================== 7, 19 -- From as-at-astonmet.com Thu Jun 12 16:12:14 2008 7, 19 -- Received: from outbound2.mail.tds.net (outbound2.mail.tds.net [216.170.230.92]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5CLCEOx008689 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 16:12:14 -0500 7, 19 -- Received: from outaamta01.mail.tds.net (outaamta01.mail.tds.net [216.170.230.31]) 7, 19 -- by outbound2.mail.tds.net (8.13.6/8.13.4) with ESMTP id m5CLCELQ030980 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 16:12:14 -0500 7, 19 -- Received: from mozart.astonmet.com ([69.11.219.4]) 7, 19 -- by outaamta01.mail.tds.net with ESMTP 7, 19 -- id {20080612211214.EKTP12265.outaamta01.mail.tds.net-at-mozart.astonmet.com} 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 16:12:14 -0500 7, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 19 -- Date: Thu, 12 Jun 2008 16:14:13 -0500 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- From: Alan Stone {as-at-astonmet.com} 7, 19 -- Subject: SEM Digital Acquisition Systems 7, 19 -- Mime-Version: 1.0 7, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 19 -- Message-Id: {20080612211214.EKTP12265.outaamta01.mail.tds.net-at-mozart.astonmet.com} ==============================End of - Headers==============================
Randy has pre-empted me while my comments have sat in the draft folder, but I'll post it anyway, now it's been written:
Given the low cost of film compared to histology or weddings, I always buy new for critical use. I assume your film is old colour slide film stock with 'free developing' included?.
The obvious thing to do is to shoot off a roll of each type and see how bad the image quality is, as it's unlikely there will be that much batch variation. Naturally you would only use the film for non-critical samples, and where you can always repeat the photos if things do go wrong. Or you can use them for training new users (where problems with focus and exposure might be even more of a problem than the old stock).
It's likely once developed this old film won't archive as well as new film either. However I've certainly used cheap 200/400 ASA 'free film' consumer quality [Konica] colour print film up to three years after it's use by date with no apparent effect on quality (which to be honest was fairly naff anyway, and I only shot off the odd roll out of interest). This cheap film shouldn't store anywhere near as well as your pro film at room temperature, and higher ASA film [200+] will always degrade quicker than low ASA film [50-100] during storage. More importantly, you shouldn't store film for long after it has been exposed (i.e. get it developed straight away).
We tended to keep the unexposed sealed slide film in the fridge rather than the freezer, as we got through the film stock quickly anyway [the only inconvenience being the 1.5 hour warm up before opening] - Kodak state store at 13oC, 4oC and -18oC without really recommending either, other than always allowing the sealed film to warm up to 21oC before opening to prevent condensation on the film surface (not that many will find a 13oC storage area). High temperature and humidity can quickly damage film, noticeably affecting picture quality, so avoid leaving film or loaded cameras in heat, direct sunlight, or in high humidity for any length of time.
The fridge/freezer doesn't protect from cosmic ray damage though - Kodak used to store film down a salt mine in a freezer to minimise film degradation from all environmental factors. I've never noticed any fogging using old sealed/stored film or any colour shift up to 3 years post use-by, although to be honest absolute colour tones weren't of much interest to me as variation in tissue stain intensity or film exposure in low light/indoors could produce far more obvious colour balance problems [mostly its poor focus that kills the photo]. However for something like RNA-ISH where image intensity and tone is critical you wouldn't use this old stock anyway. However I I've certainly never used unexposed slide film stock as old as ~9 year post-production before. I would be interested to hear how well it exposes/develops.
I've have used 15+ year old B&W Polaroid 'instant' lab film [from 1965 found left in a lab cupboard around 1982] and it's still worked adequately for it's intended use: oscilloscope traces (with the photo's being as instantly disappointing as brand new Polaroid film). We used it up more for a laugh though to see how it would work and from memory the image was rather fainter than normal and I expect this developed image would fade faster than with new film. Self developing Polariod film isn't always freezer friendly though - store that sealed in the fridge.
Polaroid's website probably says it all: "With refrigeration, we have seen outdated film provide satisfactory results; however, we cannot guarantee the image quality of outdated film".
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] Sent: 09 June 2008 18:42 To: kjmorris-at-well.ox.ac.uk
Hello Listers,
How long does 35mm film last? I have sealed individual rolls (Kodak and AGFA) expiration date 04/2001 and other older bulk rolls in their bulk loaders.
All have been kept in a drawer at room temperature. Are they still OK to use?
Thanks in advance,
Paula :-)
Paula Sicurello
VA Hospital San Diego
Microscope Facility room B141
3350 La Jolla Village Drive
San Diego, CA 92161
858-552-8585 x2397
==============================Original Headers============================== 14, 24 -- From vapatpxs-at-yahoo.com Mon Jun 9 12:30:38 2008 14, 24 -- Received: from n25.bullet.mail.mud.yahoo.com (n25.bullet.mail.mud.yahoo.com [68.142.206.220]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m59HUcZA010269 14, 24 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jun 2008 12:30:38 -0500 14, 24 -- Received: from [68.142.200.221] by n25.bullet.mail.mud.yahoo.com with NNFMP; 09 Jun 2008 17:30:37 -0000 14, 24 -- Received: from [216.252.122.217] by t9.bullet.mud.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- Received: from [69.147.65.157] by t2.bullet.sp1.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- Received: from [127.0.0.1] by omp405.mail.sp1.yahoo.com with NNFMP; 09 Jun 2008 17:30:38 -0000 14, 24 -- X-Yahoo-Newman-Property: ymail-3 14, 24 -- X-Yahoo-Newman-Id: 373802.7282.bm-at-omp405.mail.sp1.yahoo.com 14, 24 -- Received: (qmail 52867 invoked by uid 60001); 9 Jun 2008 17:30:38 -0000 14, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 14, 24 -- s=s1024; d=yahoo.com; 14, 24 -- h=Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-I D; 14, 24 -- b=MmN8GpdqMPfkJ8e1Y13HohpNQMOpWjzKhvDAKy6iQPq621WBRFjwM2JSPQdvPjBJapl8VrreH4 f6KLJ1+4DJcUkjviL94smRv0c9t6mt6SNrNJt5V8vUxipA3c5HyAzLXJ/0Lm0kVCNuFt7AvL4Alz h87wcEIvm7CawsSKXwatg=; 14, 24 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Mon, 09 Jun 2008 10:30:38 PDT 14, 24 -- X-Mailer: YahooMailWebService/0.7.199 14, 24 -- Date: Mon, 9 Jun 2008 10:30:38 -0700 (PDT) 14, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 14, 24 -- Subject: 35mm film expiration date 14, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 14, 24 -- MIME-Version: 1.0 14, 24 -- Content-Type: text/plain; charset=us-ascii 14, 24 -- Message-ID: {183151.50499.qm-at-web46101.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 33, 20 -- From kjmorris-at-well.ox.ac.uk Fri Jun 13 03:44:57 2008 33, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 33, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5D8iuHP010001 33, 20 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Jun 2008 03:44:57 -0500 33, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 33, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 33, 20 -- id 1K74tz-0003aq-Pp 33, 20 -- for Microscopy-at-Microscopy.Com; Fri, 13 Jun 2008 09:44:55 +0100 33, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 33, 20 -- To: {Microscopy-at-Microscopy.Com} 33, 20 -- Subject: FW: [Microscopy] 35mm film expiration date 33, 20 -- Date: Fri, 13 Jun 2008 09:44:55 +0100 33, 20 -- Message-ID: {224335ACADE14F56B448680D9BDA8116-at-CytoWhizz} 33, 20 -- MIME-Version: 1.0 33, 20 -- Content-Type: text/plain; 33, 20 -- charset="US-ASCII" 33, 20 -- Content-Transfer-Encoding: 7bit 33, 20 -- X-Mailer: Microsoft Office Outlook 11 33, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 33, 20 -- thread-index: AcjKWC/sSvI8ylhRQvWOtnyMrYChhQAfduYgABRy9zAAHyFPIA== ==============================End of - Headers==============================
Hi all, Does anyone have advice or experience doing TEM level double-labeling using anti-GFP and anti-RFP antibodies? I can buy mouse anti-GFP and rabbit anti-RFP and then buy the proper gold-conjugated secondary for each of those (goat anti-mouse and anti-rabbit) with different size gold particles (12 and 18 nm) but the PI is worried about cross reactivity. They want to buy the right antibodies that will give the best results.
Any advice would be greatly appreciated. my thanks, Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
*************************************************************************** The Friends of the Marine Institute - Join Today! www.friendsofugami.com
==============================Original Headers============================== 13, 19 -- From beth-at-plantbio.uga.edu Fri Jun 13 11:19:49 2008 13, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5DGJnNs008809 13, 19 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jun 2008 11:19:49 -0500 13, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 13, 19 -- (authenticated user beth-at-plantbio.uga.edu) 13, 19 -- by dogwood.plantbio.uga.edu 13, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 13, 19 -- for microscopy-at-microscopy.com; 13, 19 -- Fri, 13 Jun 2008 12:19:45 -0400 13, 19 -- Message-Id: {3AD568C9-D62B-41FA-9B23-425EF9440A80-at-plantbio.uga.edu} 13, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 13, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 13, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 19 -- Content-Transfer-Encoding: 7bit 13, 19 -- Mime-Version: 1.0 (Apple Message framework v919.2) 13, 19 -- Subject: gold-labeling question 13, 19 -- Date: Fri, 13 Jun 2008 12:19:47 -0400 13, 19 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
I use a Canon EOS-40D adapted to my stereo microscopes, and full-frame Canon cameras, such as the EOS-1Ds Mark II on my AX70 compound microscope. The images are far better than the ones I've seen from so-called scientific cameras. Breeze Systems DSLRemote software with live view is excellent for direct-to-PC capture.
I have no financial interests, etc.
} I'm looking for a C-mount color camera for under $2000 US for routine transmitted light still images(brightfield/DIC/phase). I'll not be using it for fluorescence, so I don't need something very sensitive. I've found some under-$2k models that seemingly fit the bill from the following manufacturers and was wondering if people can comment on these or suggest others: } } Pixera (Professional PIVCS10132) } Lumenera (Infinity1-3) } Pixelink (PL-A623-C and PL-B774-U) } } Also, what are people's thoughts on CMOS vs. CCD? The one CMOS camera we've tried (not named above) seemed to be more noisy even under high-light conditions (low gain) than CCD and had trouble with white-balance/color fidelity.
-- Bernard R. Cuzzillo, Ph.D., P.E. President, Mechanical Engineer, and Fire Scientist Berkeley Research Company (BRC) 600 Addison Street Berkeley, CA 94710-1920 USA
I embedded biopsy samples from three individuals in Spurrs epoxy which polymerizes only to a gummy-bear consistency. It's possible that I did not add the catalyst but I am not sure exactly what went wrong. I've polymerized overnight at 70C; then overnight at 100C; then for 60 minutes in the microwave all without additional polymerization. Does anyone have an idea on how these difficult to obtain samples might be saved?
Many thanks,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3102 S.W. Sam Jackson Park Road Portland, Oregon 97239 503-221-3434 drk-at-shcc.org
==============================Original Headers============================== 9, 26 -- From drk-at-shcc.org Fri Jun 13 12:24:24 2008 9, 26 -- Received: from mail.shcc.org (shcc.org [64.213.211.200]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5DHONNw003946 9, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Jun 2008 12:24:24 -0500 9, 26 -- Received: from por-ex-ch01.research.shcc.org (64.213.211.212) by mail.shcc.org 9, 26 -- (64.213.211.200) with Microsoft SMTP Server (TLS) id 8.0.783.2; Fri, 13 Jun 9, 26 -- 2008 10:24:23 -0700 9, 26 -- Received: from por-ex-mb01.research.shcc.org ([64.213.211.102]) by 9, 26 -- por-ex-ch01.research.shcc.org ([64.213.211.212]) with mapi; Fri, 13 Jun 2008 9, 26 -- 10:28:26 -0700 9, 26 -- From: Doug Keene {drk-at-shcc.org} 9, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 26 -- Date: Fri, 13 Jun 2008 10:28:56 -0700 9, 26 -- Subject: epon blocks won't polymerize 9, 26 -- Thread-Topic: epon blocks won't polymerize 9, 26 -- Thread-Index: AcjNevVyDxJQU+JCRwGt/Lm0xjonQA== 9, 26 -- Message-ID: {145D57516E39804A832D96C2EC1C553F1678E875F1-at-por-ex-mb01.research.shcc.org} 9, 26 -- Accept-Language: en-US 9, 26 -- Content-Language: en-US 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- acceptlanguage: en-US 9, 26 -- Content-Type: text/plain; charset="us-ascii" 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5DHONNw003946 ==============================End of - Headers==============================
I would recommend conjugating the primary antibodies directly to the colloidal gold particles rather than buying secondary conjugates. Colloidal gold is easy enough (and cheaper) to make and conjugate in the lab, and you can better control particle concentration, which correlates to labeling time. Using a primary conjugate also increases the spatial resolution of the labeling.
Daryl
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Title-Subject: [Filtered] ImmunoEM using anti-GFP and anti-RFP antibodies
Question: Hi,
I'm planning on doing ImmunoEM of two fluorescently tagged proteins using anti-GFP and anti-RFP antibodies. The ultimate goal is to do co-localization, and the backup plan is to localize only the GFP. I was wondering if anyone can share their experience with anti-GFP and/or anti-RFP antibodies for EM and if you had problems with cross-reactivity from the antibodies.
My main questions are: 1. Which anti-GFP and/or anti-RFP antibody seems to work and from which company? 2. Have you encountered cross reactivity from the antibodies or any other problems? 3. Do you have any suggestions on other factors I should consider?
We are having a tedious stain precicpitation problem since one month. We have been using our standart contrasting protocol for years and we never had a contrasting problem before. We used glass knives for sectioning within this time without any serious precipitation problem. Last month, we decided to use our new diamond knife for sectioning and after that contrasting problems started. It may sound silly, but I really wonder what can cause this problem. When our assistant took gold colored "relatively thick" sections by a hand made glass knife, contrast is ok but resolution is unsatisfactory. But when she used the diamond knife and took silver colored ultra thin sections result is a real disappointment. Precipitates obscuring all structures and preventing even diagnose of patients. Taking photograph for research studies is also impossible. We tried to contrast both kind of sections simultaneously but nothing changed. We need help, we want to use our diamond knife effectively, because it cost us a fortune :) I can send photograph samples with precipitates, if you want to examine. Thanks in advance...
Briefly protocol: Floating uranyl acetate drops for 5 mins Washing plenty of distilled water Floating lead citrate (Reynold's) for 2 mins Washing plenty of distilled water
Dr. Necat Yilmaz University of Mersin School of Medicine Histology & Embryology Dept. Mersin/TURKEY
==============================Original Headers============================== 6, 31 -- From nyilmaz-at-mersin.edu.tr Sat Jun 14 07:05:57 2008 6, 31 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5EC5rZa014612 6, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 14 Jun 2008 07:05:56 -0500 6, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 6, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 9D0A7223DC2; 6, 31 -- Sat, 14 Jun 2008 15:04:04 +0300 (EEST) 6, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 6, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 6, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 6, 31 -- with ESMTP id 8WfMfWdyUp9R; Sat, 14 Jun 2008 15:03:36 +0300 (EEST) 6, 31 -- Received: from nejat (unknown [88.224.52.50]) 6, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id DF26A223DDA; 6, 31 -- Sat, 14 Jun 2008 15:03:09 +0300 (EEST) 6, 31 -- Message-ID: {000c01c8ce16$dce840f0$0301a8c0-at-nejat} 6, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 6, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 6, 31 -- Subject: does staining artifact occur due to section thickness??? 6, 31 -- Date: Sat, 14 Jun 2008 15:04:56 +0300 6, 31 -- MIME-Version: 1.0 6, 31 -- Content-Type: text/plain; 6, 31 -- format=flowed; 6, 31 -- charset="iso-8859-9"; 6, 31 -- reply-type=original 6, 31 -- Content-Transfer-Encoding: 7bit 6, 31 -- X-Priority: 3 6, 31 -- X-MSMail-Priority: Normal 6, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 6, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 6, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 6, 31 -- X-Virus-Scanned: ClamAV using ClamSMTP ==============================End of - Headers==============================
Several of the "standard" microscopy texts talk about dealing with the inevitable problems like this. If it is truly not polymerized you can just extract with solvent like propylene oxide and the re-embed with some test-polymerized resin. There are some methods for removal of polymerized epoxy as well, but these are primarily used on sections and I don't know how long it would take to remove the resin from whole specimen-sized blocks. While it might seem harsh, I think that the chemistry is fairly well targeted to the epoxide linkages and H. Ris showed some very delicate structure using this method (on sections....).
I have had similar problems from time to time and anytime possible I keep some samples at -20C in 100% acetone and leave some at -20C in the unpolymerized resin; additionally, I usually do several resin changes including one overnight, so I put some of my resin into a Beem cap and test harden the resin overnight so I know if there is a major problem with the resin before polymerizing.
A solution for the removal of resin from epoxy sections. Tsukasa Idaware, Etsuko Harada, Shinji Yoshino, and Taizo Arai. Stain Technology 65 (205). 1990. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Stock solution (aka "Treating solution"): 1.3g 18-crown-6 ether Aldrich #16,665-1 or Sigma # C5515 99 ml DMSO 1 ml water
Working Solution: (prepare fresh daily) 3 ml 30% methanolic potasium methoxide (turbid white; stir to mix, then draw 3 ml) 100 ml Stock solution (above)
Sections are attached to "aminosilane" treated glass coverslips or slides. Mix the "working solution" - should become clear when mixed. Place the glass with sections into the working solution. Leave for 5 min, swirling once a minute. Withdraw the glass and place section side up into distilled water. Wash gently with several changes of dH2O.
Various treatments are now possible: - dehydrate and CPD; sputter coat for SEM. - immunolocalization?
Hans Ris used a Polysciences epoxy removal kit which is essentially the Idaware formula.
Ris dehydrates 50%, 70%, 80%, and 95% ethanol, and 2x in dry 100% ethanol. He sputters with ~1nm of platinum for high resolution FESEM observation (Hitachi S-900 FESEM at 1.5 kV; this is an in-lens system...)
The paper does not state how the methanolic potassium methoxide is prepared, but it is probably like the sodium ethoxide formulas below.
Sodium Ethoxide formulas: Ethanol is dehydrated over 3A molecular sieves. Ethanolic NaOH, 3%, is prepared by dissolving 3g NaOH in 97g anhydrous ethanol. 10% ethanolic NaOH is prepared similarly. ==========================================================
Dale
drk-at-shcc.org wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear Fellow Microscopists, } } I embedded biopsy samples from three individuals in Spurrs epoxy which polymerizes only to a gummy-bear consistency. It's possible that I did not add the catalyst but I am not sure exactly what went wrong. I've polymerized overnight at 70C; then overnight at 100C; then for 60 minutes in the microwave all without additional polymerization. Does anyone have an idea on how these difficult to obtain samples might be saved? } } Many thanks, } } Doug } } } Douglas R. Keene } Assistant Investigator } Micro-Imaging Center } Shriners Hospital for Children } 3102 S.W. Sam Jackson Park Road } Portland, Oregon 97239 } 503-221-3434 } drk-at-shcc.org } } } } ==============================Original Headers============================== } 9, 26 -- From drk-at-shcc.org Fri Jun 13 12:24:24 2008 } 9, 26 -- Received: from mail.shcc.org (shcc.org [64.213.211.200]) } 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5DHONNw003946 } 9, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Jun 2008 12:24:24 -0500 } 9, 26 -- Received: from por-ex-ch01.research.shcc.org (64.213.211.212) by mail.shcc.org } 9, 26 -- (64.213.211.200) with Microsoft SMTP Server (TLS) id 8.0.783.2; Fri, 13 Jun } 9, 26 -- 2008 10:24:23 -0700 } 9, 26 -- Received: from por-ex-mb01.research.shcc.org ([64.213.211.102]) by } 9, 26 -- por-ex-ch01.research.shcc.org ([64.213.211.212]) with mapi; Fri, 13 Jun 2008 } 9, 26 -- 10:28:26 -0700 } 9, 26 -- From: Doug Keene {drk-at-shcc.org} } 9, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 9, 26 -- Date: Fri, 13 Jun 2008 10:28:56 -0700 } 9, 26 -- Subject: epon blocks won't polymerize } 9, 26 -- Thread-Topic: epon blocks won't polymerize } 9, 26 -- Thread-Index: AcjNevVyDxJQU+JCRwGt/Lm0xjonQA== } 9, 26 -- Message-ID: {145D57516E39804A832D96C2EC1C553F1678E875F1-at-por-ex-mb01.research.shcc.org} } 9, 26 -- Accept-Language: en-US } 9, 26 -- Content-Language: en-US } 9, 26 -- X-MS-Has-Attach: } 9, 26 -- X-MS-TNEF-Correlator: } 9, 26 -- acceptlanguage: en-US } 9, 26 -- Content-Type: text/plain; charset="us-ascii" } 9, 26 -- MIME-Version: 1.0 } 9, 26 -- Content-Transfer-Encoding: 8bit } 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5DHONNw003946 } ==============================End of - Headers==============================
==============================Original Headers============================== 16, 20 -- From dac-at-research.umass.edu Sat Jun 14 07:17:08 2008 16, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 16, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5ECH8T9027481 16, 20 -- for {microscopy-at-microscopy.com} ; Sat, 14 Jun 2008 07:17:08 -0500 16, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 16, 20 -- (authenticated bits=0) 16, 20 -- by race5.oit.umass.edu (8.14.3/8.14.1) with ESMTP id m5ECH7gk001715 16, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 16, 20 -- for {microscopy-at-microscopy.com} ; Sat, 14 Jun 2008 08:17:07 -0400 16, 20 -- Message-ID: {4853C502.5000001-at-research.umass.edu} 16, 20 -- Date: Sat, 14 Jun 2008 08:17:54 -0500 16, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 16, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 16, 20 -- MIME-Version: 1.0 16, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 16, 20 -- Subject: Re: [Microscopy] epon blocks won't polymerize 16, 20 -- References: {200806131729.m5DHTSNY012405-at-ns.microscopy.com} 16, 20 -- In-Reply-To: {200806131729.m5DHTSNY012405-at-ns.microscopy.com} 16, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 16, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Robert Apkarian Integrated Electron Microscopy Core at the Emory University is seeking to hire a full time staff member. This is a newly consolidated university wide research resource core with both TEM and SEM capabilities. The applicants should have a Bachelor's degree in a scientific field or equivalent combination of experience, education and training, and should have good people skills as the job requires daily interaction with core users. It is desired (but not required) that the candidate would have 2-3 years of experience with electron microscopy, especially biological TEM. Experience/knowledge in immunocytochemistry and cryo techniques would be advantageous.
For more information regarding this opening, please contact Hong Yi at (404) 712-8491 or hyi-at-emory.edu. Thank you.
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====================== Hong Yi Technical Director Robert Apkarian Integrated Electron Microscopy Core Suite E106 Cherry L. Emerson Hall 1515 Dickey Drive, NE Emory University Atlanta, GA 30322
Hi Necat, } } Briefly protocol: } Floating uranyl acetate drops for 5 mins } Washing plenty of distilled water } Floating lead citrate (Reynold's) for 2 mins } Washing plenty of distilled water
Once I got rather similar problem wit precipitate and I could not trace it to any conditions for half a year. It was very frustrating! However, the problem was in lead citrate step. Do not float you grids on the droplet but put them inside (let them sink). In this way grids will not have a contact with atmospheric carbon dioxide that can cause rather nasty precipitate. Wash the grids after stain by holding them with twizers and soaking into the beaker with distilled water 30-40 times. Then just blot the grid with filter paper.
==============================Original Headers============================== 4, 33 -- From Aleksandr.Mironov-at-manchester.ac.uk Sun Jun 15 11:46:28 2008 4, 33 -- Received: from clarity.mcc.ac.uk (clarity.mcc.ac.uk [130.88.200.144]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5FGkQvo014400 4, 33 -- for {microscopy-at-microscopy.com} ; Sun, 15 Jun 2008 11:46:28 -0500 4, 33 -- Received: from webmail-w4.its.manchester.ac.uk ([10.2.61.7]) 4, 33 -- by clarity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD)) 4, 33 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 4, 33 -- id 1K7vPi-000Fqj-P5; Sun, 15 Jun 2008 17:49:10 +0100 4, 33 -- Received: by webmail-w4.its.manchester.ac.uk (Postfix, from userid 30) 4, 33 -- id B6A031000048; Sun, 15 Jun 2008 17:49:10 +0100 (BST) 4, 33 -- Received: from cpc1-bagu7-0-0-cust534.bagu.cable.ntl.com 4, 33 -- (cpc1-bagu7-0-0-cust534.bagu.cable.ntl.com [80.2.178.23]) by 4, 33 -- webmail.manchester.ac.uk (Horde MIME library) with HTTP; Sun, 15 Jun 2008 4, 33 -- 17:49:10 +0100 4, 33 -- Message-ID: {20080615174910.89r41547b4g00gsg-at-webmail.manchester.ac.uk} 4, 33 -- X-Priority: 3 (Normal) 4, 33 -- Date: Sun, 15 Jun 2008 17:49:10 +0100 4, 33 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} 4, 33 -- To: nyilmaz-at-mersin.edu.tr 4, 33 -- Cc: microscopy-at-microscopy.com 4, 33 -- Subject: Re: [Microscopy] does staining artifact occur due to section 4, 33 -- thickness??? 4, 33 -- References: {200806141212.m5ECCc8k023438-at-ns.microscopy.com} 4, 33 -- In-Reply-To: {200806141212.m5ECCc8k023438-at-ns.microscopy.com} 4, 33 -- MIME-Version: 1.0 4, 33 -- Content-Type: text/plain; 4, 33 -- charset=UTF-8; 4, 33 -- DelSp="Yes"; 4, 33 -- format="flowed" 4, 33 -- Content-Disposition: inline 4, 33 -- Content-Transfer-Encoding: 7bit 4, 33 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.6) 4, 33 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. ==============================End of - Headers==============================
The most likely causes are old accelerator or incorrectly mixed resin. If you are using the "new" ERL 4221 instead of 4206 the quantities may be wrong in the mixing instructions. A straight 1:1 substitution will not do. See the work of E. Anne Ellis for the correct recipe. For your current samples, dissolve out the "bad" resin and re-embed in fresh and correctly mixed resin.
Geoff
drk-at-shcc.org wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear Fellow Microscopists, } } I embedded biopsy samples from three individuals in Spurrs epoxy which polymerizes only to a gummy-bear consistency. It's possible that I did not add the catalyst but I am not sure exactly what went wrong. I've polymerized overnight at 70C; then overnight at 100C; then for 60 minutes in the microwave all without additional polymerization. Does anyone have an idea on how these difficult to obtain samples might be saved? } } Many thanks, } } Doug } } } Douglas R. Keene } Assistant Investigator } Micro-Imaging Center } Shriners Hospital for Children } 3102 S.W. Sam Jackson Park Road } Portland, Oregon 97239 } 503-221-3434 } drk-at-shcc.org } } } } ==============================Original Headers============================== } 9, 26 -- From drk-at-shcc.org Fri Jun 13 12:24:24 2008 } 9, 26 -- Received: from mail.shcc.org (shcc.org [64.213.211.200]) } 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5DHONNw003946 } 9, 26 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Jun 2008 12:24:24 -0500 } 9, 26 -- Received: from por-ex-ch01.research.shcc.org (64.213.211.212) by mail.shcc.org } 9, 26 -- (64.213.211.200) with Microsoft SMTP Server (TLS) id 8.0.783.2; Fri, 13 Jun } 9, 26 -- 2008 10:24:23 -0700 } 9, 26 -- Received: from por-ex-mb01.research.shcc.org ([64.213.211.102]) by } 9, 26 -- por-ex-ch01.research.shcc.org ([64.213.211.212]) with mapi; Fri, 13 Jun 2008 } 9, 26 -- 10:28:26 -0700 } 9, 26 -- From: Doug Keene {drk-at-shcc.org} } 9, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 9, 26 -- Date: Fri, 13 Jun 2008 10:28:56 -0700 } 9, 26 -- Subject: epon blocks won't polymerize } 9, 26 -- Thread-Topic: epon blocks won't polymerize } 9, 26 -- Thread-Index: AcjNevVyDxJQU+JCRwGt/Lm0xjonQA== } 9, 26 -- Message-ID: {145D57516E39804A832D96C2EC1C553F1678E875F1-at-por-ex-mb01.research.shcc.org} } 9, 26 -- Accept-Language: en-US } 9, 26 -- Content-Language: en-US } 9, 26 -- X-MS-Has-Attach: } 9, 26 -- X-MS-TNEF-Correlator: } 9, 26 -- acceptlanguage: en-US } 9, 26 -- Content-Type: text/plain; charset="us-ascii" } 9, 26 -- MIME-Version: 1.0 } 9, 26 -- Content-Transfer-Encoding: 8bit } 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5DHONNw003946 } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 28 -- From mcauliff-at-umdnj.edu Mon Jun 16 08:57:01 2008 8, 28 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GDv0LC000624 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 08:57:00 -0500 8, 28 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 8, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 890FA4BE4E 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 09:59:14 -0400 (EDT) 8, 28 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 8, 28 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 1498CA3B71 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 09:59:10 -0400 (EDT) 8, 28 -- Received: from ([10.32.15.167]) 8, 28 -- by imail.umdnj.edu with ESMTP id 5502050.18290124; 8, 28 -- Mon, 16 Jun 2008 09:58:53 -0400 8, 28 -- MIME-version: 1.0 8, 28 -- Content-transfer-encoding: 7BIT 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 8, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 8, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0K2K00FY26U45L60-at-umduwc01.umdnj.edu} for 8, 28 -- microscopy-at-microscopy.com; Mon, 16 Jun 2008 09:58:53 -0400 (EDT) 8, 28 -- Message-id: {485671C8.2020002-at-umdnj.edu} 8, 28 -- Date: Mon, 16 Jun 2008 09:59:36 -0400 8, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 28 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 8, 28 -- To: drk-at-shcc.org, microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] epon blocks won't polymerize 8, 28 -- References: {200806131725.m5DHPStm005488-at-ns.microscopy.com} 8, 28 -- In-reply-to: {200806131725.m5DHPStm005488-at-ns.microscopy.com} ==============================End of - Headers==============================
The references I think Geoff refers to are from Microscopy Today:
July 2006, 14(4) Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations. E. Ann Ellis September 2006, 14(5) A Simplified Method for Formulation of Epoxy Resin Embedding Media.
If you don't have these, they can be downloaded as pdf files from the website: http://www.microscopy-today.com/MTSelectTOC.html
You can only get entire issues, not separate articles, but that shouldn't take long with an ethernet connection.
Phil
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The most likely causes are old accelerator or incorrectly mixed resin. If you are using the "new" ERL 4221 instead of 4206 the quantities may be wrong in the mixing instructions. A straight 1:1 substitution will not do. See the work of E. Anne Ellis for the correct recipe. For your current samples, dissolve out the "bad" resin and re-embed in fresh and correctly mixed resin.
Geoff
drk-at-shcc.org wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear Fellow Microscopists, } } I embedded biopsy samples from three individuals in Spurrs epoxy } which polymerizes only to a gummy-bear consistency. It's possible } that I did not add the catalyst but I am not sure exactly what went } wrong. I've polymerized overnight at 70C; then overnight at 100C; } then for 60 minutes in the microwave all without additional } polymerization. Does anyone have an idea on how these difficult to } obtain samples might be saved? } } Many thanks, } } Doug } } } Douglas R. Keene } Assistant Investigator } Micro-Imaging Center } Shriners Hospital for Children } 3102 S.W. Sam Jackson Park Road } Portland, Oregon 97239 } 503-221-3434 } drk-at-shcc.org } -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 10, 20 -- From oshel1pe-at-cmich.edu Mon Jun 16 09:44:47 2008 10, 20 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GEilHR015204 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 09:44:47 -0500 10, 20 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 10, 20 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m5GEwA7C022634 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 10:58:10 -0400 10, 20 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 10, 20 -- Mon, 16 Jun 2008 10:47:51 -0400 10, 20 -- Mime-Version: 1.0 10, 20 -- Message-Id: {f06240808c47c2a1c9214-at-[141.209.160.249]} 10, 20 -- Date: Mon, 16 Jun 2008 10:46:54 -0400 10, 20 -- To: Microscopy-at-microscopy.com 10, 20 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 10, 20 -- Subject: Re: epon blocks won't polymerize 10, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 10, 20 -- X-OriginalArrivalTime: 16 Jun 2008 14:47:51.0642 (UTC) FILETIME=[F3D27BA0:01C8CFBF] 10, 20 -- X-CanItPRO-Stream: default 10, 20 -- X-Spam-Score: -4 () L_EXCH_MF 10, 20 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Reynolds did that to me also at times. Check out Sato's lead stain for a formulation that does not do this. We've used it for over 30 years now. Here is from my and Jan Factor's May 3 2006 posting to the list: Sato's lead stain is a more stable replacement for Reynolds Pb citrate. We've used it since the 1970s. 1968 Sato, T.: J. Electron Microsc. 17:158, 1968. 1968 Sato and others: Proc. XIth Int. Cong. on Electron Microscopy. Kyoto. 1986, pp. 2181-2182. 1968 Takamasa Hanaichi et al. A Stable Lead by Modification of Sato's Method. J. Electron Microsc., Vol. 35. No. 3. 304-306. There were a cluster of postings in May 2006, if you can search under Subject: [Microscopy] TEM--Lead Citrate--HELP!!
the most helpful about calcined lead citrate were Jan Factor's second May 3, 2006 posting and Stephane Nizet's of June 7, 2006.
-mike-
At 11:51 AM -0500 6/15/08, Aleksandr.Mironov-at-manchester.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
As someone who has embedded thousands of muscle biopsies, I would suggest that Spurr's is not the best choice. "Epon" type resin mixes are extremely reliable and have excellent staining and cutting characteristics. My standard mixture is Polybed 812: Araldite 502: DDSA in the proportion 5:4:12 by volume (no commercial interest in products). Aliquots can be frozen and used when needed, with DMP added at time of use. I use Spurr's only for special purposes -- plant material, fungi, parasites, etc.
Were the bad blocks embedded in flat molds or in Beem capsules? Spurr's does not polymerize well if the humidity is high. If using flat molds, put them in sealed plastic containers with some Drierite.
Ralph Common Michigan State University
==============================Original Headers============================== 5, 24 -- From rcommon-at-msu.edu Mon Jun 16 10:12:36 2008 5, 24 -- Received: from sys31.mail.msu.edu (sys31.mail.msu.edu [35.9.75.131]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GFCado009156 5, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 10:12:36 -0500 5, 24 -- Received: from [35.9.122.125] (helo=emlab) 5, 24 -- by sys31.mail.msu.edu with esmtpsa (Exim 4.63 #1) 5, 24 -- (TLSv1:RC4-MD5:128) 5, 24 -- id 1K8GPv-0003wM-79 5, 24 -- for Microscopy-at-microscopy.com; Mon, 16 Jun 2008 11:14:47 -0400 5, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 5, 24 -- To: {Microscopy-at-microscopy.com} 5, 24 -- Subject: epon blocks won't polymerize 5, 24 -- Date: Mon, 16 Jun 2008 11:14:52 -0400 5, 24 -- Message-ID: {003601c8cfc3$bb3deff0$7d7a0923-at-msu.edu} 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="iso-8859-1" 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Priority: 3 (Normal) 5, 24 -- X-MSMail-Priority: Normal 5, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 5, 24 -- Importance: Normal 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1914 5, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Lockheed Martin- KAPL, Inc. has an open position for a surface scientist (XPS/ESCA-Auger-SIMS). NOTE: US Citizenship REQUIRED.
A brief description of the job is given below. The complete job announcement (Req ID 81677BR) can be found at the Lockheed Martin career web site (LockheedMartin.com).
Thanks.
Jim McGee ************************************ James J. McGee Materials Engineer, Test Operations Lockheed Martin, KAPL, Inc. Mail Bin 149 PO Box 1072 Schenectady, NY 12301-1072
Req ID 81677BR Industry Job Title - Materials Engineer Sr
Required skills - MS degree in the physical sciences (e.g. materials science, chemistry, geology, solid state physics) or engineering, plus hands-on training and/or experience operating Electron Spectroscopy for Chemical Analysis (ESCA), Secondary Ion Mass Spectrometry (SIMS), and scanning Auger electron microscopy (SAM) instruments.
Desired skills - PhD degree in physical sciences with 5 years or more hands-on experience in surface and microanalysis techniques(ESCA, SAM, and SIMS). Demonstrated problem solving experience in areas of metallurgy, alloy testing/development, solid inorganic materials, and/or ceramics. Previous experience and nuclear or radioactive materials, corrosion, metallurgy, and ceramics is a also desirable.
Specific Job Description - Characterize the surfaces of materials to support environmental testing and failure analysis of in-service components. Utilize ESCA spectroscopy and imaging, SIMS, and field-emission Auger electron microscopy and imaging techniques. Execute data and image processing (e.g. Target-Factor Analysis (TFA, PCA), curve fitting, quantitative analysis, data assimilation, and reporting of results. Participate in multidisciplinary, collaborative investigations and team-oriented problem solving with other materials characterization specialists and materials scientists/engineers using associated analytical techniques (FEG-SEM, EBSD, EPMA, XRD, TEM, FTIR, Raman, FIB, TOF-SIMS).
The major duties will be the use of advanced surface analytical techniques (e.g. ESCA, Auger, and SIMS) to determine the microstructure and microchemistry of metals, alloys, and ceramic materials. Ensure instrumentation is properly maintained and in proper operating condition. Analyze and interpret data and communicate results to sponsoring groups by oral and written reports. Serve as part of research teams to design experiments that will elucidate structure-composition-property-processing relationships. Stay current in surface and microanalytical techniques and applications and propose/implement new capabilities or characterization tools.
Applicants selected will be subject to a Federal background investigation and must meet eligibility requirements for access to classified matter. US citizenship is required.
==============================Original Headers============================== 13, 28 -- From mcgeejj-at-kapl.gov Mon Jun 16 10:21:26 2008 13, 28 -- Received: from ibetpms06.bias2000.bettis.gov (bettis.gov [65.170.176.212]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GFLPix016533 13, 28 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jun 2008 10:21:26 -0500 13, 28 -- Received: from atlas.bias2000.bettis.gov (unverified) by ibetpms06.bias2000.bettis.gov 13, 28 -- (Clearswift SMTPRS 5.2.9) with SMTP id {T879d868447ac100286148c-at-ibetpms06.bias2000.bettis.gov} for {microscopy-at-microscopy.com} ; 13, 28 -- Mon, 16 Jun 2008 11:23:43 -0400 13, 28 -- Received: from ibetpex02.bias2000.bettis.gov ([172.16.2.129]) by atlas.bias2000.bettis.gov with Microsoft SMTPSVC(6.0.3790.1830); 13, 28 -- Mon, 16 Jun 2008 11:23:43 -0400 13, 28 -- Content-class: urn:content-classes:message 13, 28 -- MIME-Version: 1.0 13, 28 -- Content-Type: text/plain; 13, 28 -- charset="us-ascii" 13, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 28 -- Subject: Open Position - Surface scientist for materials science applications 13, 28 -- Date: Mon, 16 Jun 2008 11:23:43 -0400 13, 28 -- Message-ID: {8807BA5B50FE0E40957843C1A36ACB833D9E2C-at-ibetpex02.bias2000.bettis.gov} 13, 28 -- In-Reply-To: {200802081429.m18ETQAX001375-at-ns.microscopy.com} 13, 28 -- X-MS-Has-Attach: 13, 28 -- X-MS-TNEF-Correlator: 13, 28 -- Thread-Topic: Open Position - Surface scientist for materials science applications 13, 28 -- Thread-Index: AchqXxXPRHPu66ctRva/pBay4ur2bxlZRNeg 13, 28 -- References: {200802081429.m18ETQAX001375-at-ns.microscopy.com} 13, 28 -- From: "McGee, James" {mcgeejj-at-kapl.gov} 13, 28 -- To: {microscopy-at-microscopy.com} 13, 28 -- X-OriginalArrivalTime: 16 Jun 2008 15:23:43.0267 (UTC) FILETIME=[F64A6730:01C8CFC4] 13, 28 -- Content-Transfer-Encoding: 8bit 13, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5GFLPix016533 ==============================End of - Headers==============================
With all the EM Labs out there, I'm curious as to which Service Engineers/Companies you'd recommend for repairing Ultramicrotomes? (especially Reichert Ultracut E's and Ultracut S's). We are all pinching pennies, (even in the Federal Government), but great service is worth the expense to keep our equipment in good working condition.
I'd be happy to compile a summary of your recommendations to share with the list.
Thank you, Virginia -- Virginia Tanner Crocker Biologist NIH, NINDS EM Facility MSC4477 Bldg. 49, Room 3C58 49 Convent Drive Bethesda, MD 20892 301-402-1568 301-480-1485 (fax) crockerv-at-ninds.nih.gov (email)
==============================Original Headers============================== 4, 17 -- From crockerv-at-ninds.nih.gov Mon Jun 16 10:51:44 2008 4, 17 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GFpibn003803 4, 17 -- for {MICROSCOPY-at-sparc5.microscopy.com} ; Mon, 16 Jun 2008 10:51:44 -0500 4, 17 -- X-IronPortListener: NIH_Relay 4, 17 -- X-SBRS: None 4, 17 -- X-IronPort-AV: E=Sophos;i="4.27,653,1204520400"; 4, 17 -- d="scan'208";a="197518381" 4, 17 -- Received: from unknown (HELO [165.112.21.103]) ([128.231.90.83]) 4, 17 -- by nihrelayxway4.hub.nih.gov with ESMTP; 16 Jun 2008 11:53:54 -0400 4, 17 -- Mime-Version: 1.0 4, 17 -- Message-Id: {p0623090dc47c3a317c9f-at-[165.112.21.103]} 4, 17 -- Date: Mon, 16 Jun 2008 11:53:54 -0400 4, 17 -- To: MICROSCOPY-at-ns.microscopy.com 4, 17 -- From: Virginia Crocker {crockerv-at-ninds.nih.gov} 4, 17 -- Subject: Service Engineers for Reichert Ultracut E's 4, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We use MOC Microscopical Optical Consulting inc http://mocleica.com/ (845) 268-6450
We are happy with their work. They are located in NY state. You may be able to find someone more local to save on the cost of the service call.
Cheers, KD Derr -----Original Message----- X-from: crockerv-at-ninds.nih.gov [mailto:crockerv-at-ninds.nih.gov] Sent: Monday, June 16, 2008 11:54 AM To: kderr-at-nysbc.org
Good Morning,
With all the EM Labs out there, I'm curious as to which Service Engineers/Companies you'd recommend for repairing Ultramicrotomes? (especially Reichert Ultracut E's and Ultracut S's). We are all pinching pennies, (even in the Federal Government), but great service is worth the expense to keep our equipment in good working condition.
I'd be happy to compile a summary of your recommendations to share with the list.
Thank you, Virginia -- Virginia Tanner Crocker Biologist NIH, NINDS EM Facility MSC4477 Bldg. 49, Room 3C58 49 Convent Drive Bethesda, MD 20892 301-402-1568 301-480-1485 (fax) crockerv-at-ninds.nih.gov (email)
==============================Original Headers============================== 4, 17 -- From crockerv-at-ninds.nih.gov Mon Jun 16 10:51:44 2008 4, 17 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 4, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GFpibn003803 4, 17 -- for {MICROSCOPY-at-sparc5.microscopy.com} ; Mon, 16 Jun 2008 10:51:44 -0500 4, 17 -- X-IronPortListener: NIH_Relay 4, 17 -- X-SBRS: None 4, 17 -- X-IronPort-AV: E=Sophos;i="4.27,653,1204520400"; 4, 17 -- d="scan'208";a="197518381" 4, 17 -- Received: from unknown (HELO [165.112.21.103]) ([128.231.90.83]) 4, 17 -- by nihrelayxway4.hub.nih.gov with ESMTP; 16 Jun 2008 11:53:54 -0400 4, 17 -- Mime-Version: 1.0 4, 17 -- Message-Id: {p0623090dc47c3a317c9f-at-[165.112.21.103]} 4, 17 -- Date: Mon, 16 Jun 2008 11:53:54 -0400 4, 17 -- To: MICROSCOPY-at-ns.microscopy.com 4, 17 -- From: Virginia Crocker {crockerv-at-ninds.nih.gov} 4, 17 -- Subject: Service Engineers for Reichert Ultracut E's 4, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 14, 22 -- From kderr-at-nysbc.org Mon Jun 16 11:19:10 2008 14, 22 -- Received: from nysbc.org (nysbc.org [74.211.206.2]) 14, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5GGJAZH017362 14, 22 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 Jun 2008 11:19:10 -0500 14, 22 -- Received: from CRYOEM03 ([10.10.10.1]) 14, 22 -- by nysbc.org (Kerio MailServer 6.5.0); 14, 22 -- Mon, 16 Jun 2008 12:21:11 -0400 14, 22 -- From: "kd Derr" {kderr-at-nysbc.org} 14, 22 -- To: {crockerv-at-ninds.nih.gov} , {Microscopy-at-Microscopy.Com} 14, 22 -- Subject: RE: [Microscopy] Service Engineers for Reichert Ultracut E's 14, 22 -- Date: Mon, 16 Jun 2008 12:20:29 -0400 14, 22 -- Message-ID: {001501c8cfcc$e48ddec0$7f05a8c0-at-CRYOEM03} 14, 22 -- MIME-Version: 1.0 14, 22 -- Content-Type: text/plain; 14, 22 -- charset="us-ascii" 14, 22 -- Content-Transfer-Encoding: 7bit 14, 22 -- X-Priority: 3 (Normal) 14, 22 -- X-MSMail-Priority: Normal 14, 22 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 14, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1914 14, 22 -- In-Reply-To: {200806161554.m5GFsD3b008692-at-ns.microscopy.com} 14, 22 -- Importance: Normal ==============================End of - Headers==============================
Virginia, We've been very pleased with John Petz and Tek-Net Inc, Lakewood NJ, for 20+ years, most recently about 5 months ago when he performed servic and repairs on 3 UltraCut E's and one precious Reichert OMU3 for us. See http://www.teknetinc.com/2682/index.html
-mike reedy-
At 10:54 AM -0500 6/16/08, crockerv-at-ninds.nih.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I am planning to change the LaB6 filament on our Carl-Zeiss EVO 40. ...(Our service people refuse to assist as we did not buy the replacement LaB6 filament from the OEM)...
I did do W-filament replacements on this machine, but first time with the LaB6. Does anyone have a written procedure that might provide some details ? Other suggestions and pointers will be much appreciated as well.
I'm going to chime in and side with Bernard on this one. We've been using the Nikon DSLR cameras for awhile now on our LM's and have been quite happy with them. We just bought a D300 and love it for a number of reasons (1) Auto exposure works very nicely (Aperture Priority), (2) You can set Delayed Shutter - Mirror up, 2 seconds, shutter snaps (reduces system vibrations, (3) Shutter and mirror slap are insignificant anyway, (4) CMOS chip (i.e. film plane) is Parfocal with Eye pieces!, (5) Live video out for through software on computer screen focusing, (6) live HTDV out for focusing on a dedicated monitor (with sub-chip zoom for focusing, i.e. the chip has more pixels than the HDTV does, and nearly any LCD monitor with DVI input will support HDTV), (7) completely operable from a computer, (8) Chip is RGB array but with 12.3 million effective pixels (13.1 MP actual), (9) Time-lapse through the software is three clicks (interval, duration, go), (10) camera cost is Aprox $1650 USD.
We have gone with Nikon rather than Canon, simply because we had Nikon film cameras previously, thus had lenses and couplings already. We to are running AX-70, SZX-12, and BX-61 scopes, for BF, DF, DIC, and fluoresence. (For comparison we also have Photometrics K-4 and Spot Cameras).
Esteban: The only failure on your requirments for the DSLR's is they are NOT C-mount. They do require the larger SLR mounts (Nikon F- mount, Canon EF / EF-S lens mount). Although you can get C to F- mount adapters.
Good luck!
On 13 Jun 2008 at 12:33, bernard-at-berkeleyrc.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I use a Canon EOS-40D adapted to my stereo microscopes, and full-frame } Canon cameras, such as the EOS-1Ds Mark II on my AX70 compound } microscope. The images are far better than the ones I've seen from } so-called scientific cameras. Breeze Systems DSLRemote software with } live view is excellent for direct-to-PC capture. } } I have no financial interests, etc. } } } } I'm looking for a C-mount color camera for under $2000 US for } routine transmitted light still images(brightfield/DIC/phase). I'll } not be using it for fluorescence, so I don't need something very } sensitive. I've found some under-$2k models that seemingly fit the } bill from the following manufacturers and was wondering if people can } comment on these or suggest others:
} } Pixera (Professional PIVCS10132) } Lumenera (Infinity1-3) } Pixelink } (PL-A623-C and PL-B774-U) } } Also, what are people's thoughts on CMOS } vs. CCD? The one CMOS camera we've tried (not named above) seemed to } be more noisy even under high-light conditions (low gain) than CCD and } had trouble with white-balance/color fidelity. } } -- } Bernard R. Cuzzillo, Ph.D., P.E. } President, Mechanical Engineer, and Fire Scientist } Berkeley Research Company (BRC) } 600 Addison Street } Berkeley, CA 94710-1920 } USA } } www.berkeleyrc.com } } bernard-at-berkeleyrc.com } } Cell phone: 510.821.2499 } Office phone: 510.868.4333 }
} ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 13, 25 -- From edelmare-at-muohio.edu Tue Jun 17 07:45:04 2008 13, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5HCj43u004716 13, 25 -- for {microscopy-at-Microscopy.com} ; Tue, 17 Jun 2008 07:45:04 -0500 13, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 13, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m5HCkQUR012560; 13, 25 -- Tue, 17 Jun 2008 08:46:26 -0400 13, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 13, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m5HCkQqF002898; 13, 25 -- Tue, 17 Jun 2008 08:46:26 -0400 13, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 13, 25 -- To: microscopy-at-Microscopy.com 13, 25 -- Date: Tue, 17 Jun 2008 08:46:26 -0400 13, 25 -- MIME-Version: 1.0 13, 25 -- Subject: Re: [Microscopy] Re: LM: Opinions on color cameras for brightfield; CMOS vs. CCD 13, 25 -- CC: "bernard-at-berkeleyrc.com" {bernard-at-berkeleyrc.com} 13, 25 -- Message-ID: {485779E2.22372.E26947E-at-edelmare.muohio.edu} 13, 25 -- Priority: normal 13, 25 -- In-reply-to: {200806131633.m5DGXImW022924-at-ns.microscopy.com} 13, 25 -- References: {200806131633.m5DGXImW022924-at-ns.microscopy.com} 13, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 13, 25 -- Content-type: text/plain; charset=US-ASCII 13, 25 -- Content-transfer-encoding: 7BIT 13, 25 -- Content-description: Mail message body 13, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
I am having problems with getting the numbers out of an automatic EDX quantification routine in FEI's ESVision version 5.0.
In the older versions of ESVision, after doing a Peak ID, Correct Background, Fit Peaks and Quantify, the compositions used to be displayed in the Output Window. In the latest version, they're not displayed here at all (although other details like Background Settings, Peak Fitting Settings and Quantification Settings are). What is goingf on? Where are the numbers displayed? The Help manual is next to useless on solving this one.
Yours, Jon Barnard
==============================Original Headers============================== 4, 24 -- From jsb43-at-hermes.cam.ac.uk Tue Jun 17 09:04:18 2008 4, 24 -- Received: from ppsw-1.csi.cam.ac.uk (ppsw-1.csi.cam.ac.uk [131.111.8.131]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5HE4IQU022871 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 09:04:18 -0500 4, 24 -- X-Cam-SpamDetails: Not scanned 4, 24 -- X-Cam-AntiVirus: No virus found 4, 24 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 4, 24 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:60695) 4, 24 -- by ppsw-1.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.151]:25) 4, 24 -- with esmtpa (EXTERNAL:jsb43) id 1K8boU-0004Rp-4V (Exim 4.67) for Microscopy-at-microscopy.com 4, 24 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Tue, 17 Jun 2008 15:05:34 +0100 4, 24 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 4, 24 -- with local (PRAYER:jsb43) id 1K8boU-0002h6-CM (Exim 4.67) for Microscopy-at-microscopy.com 4, 24 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Tue, 17 Jun 2008 15:05:34 +0100 4, 24 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 4, 24 -- To: MSA Listserver {Microscopy-at-microscopy.com} 4, 24 -- Subject: ESVision: output of EDX quantification 4, 24 -- Date: 17 Jun 2008 15:05:34 +0100 4, 24 -- X-Mailer: Prayer v1.2.2.1 4, 24 -- X-Originating-IP: [131.111.102.18] 4, 24 -- Message-ID: {Prayer.1.2.2.1.0806171505340.4840-at-hermes-1.csi.cam.ac.uk} 4, 24 -- Mime-Version: 1.0 4, 24 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 4, 24 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
We also had a mystery precipitate problem in our lab for a LOONG time that defeated all attempts to beat it, until our director suggested adding 2-mercaptoethanol to our processing steps before, during and after osmium fixation. Problem solved, but cause unknown. I have formerly posted a couple other emails about this to the list.
This might not relate to your specific problem, but it's worth a try if you have Intractable Pepper Syndrome and nothing else works. We have a protocol on our website at http://www.emc.missouri.edu/Pdfs/General%202-ME%20Microwave%20Processing %20Protocol.pdf. You can easily adapt this to non-microwave processing.
Good luck!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: Aleksandr.Mironov-at-manchester.ac.uk [mailto:Aleksandr.Mironov-at-manchester.ac.uk] Sent: Sunday, June 15, 2008 11:49 AM To: Tindall, Randy D.
Hi Necat, } } Briefly protocol: } Floating uranyl acetate drops for 5 mins Washing plenty of distilled } water Floating lead citrate (Reynold's) for 2 mins Washing plenty of } distilled water
Once I got rather similar problem wit precipitate and I could not trace it to any conditions for half a year. It was very frustrating! However, the problem was in lead citrate step. Do not float you grids on the droplet but put them inside (let them sink). In this way grids will not have a contact with atmospheric carbon dioxide that can cause rather nasty precipitate. Wash the grids after stain by holding them with twizers and soaking into the beaker with distilled water 30-40 times. Then just blot the grid with filter paper.
==============================Original Headers============================== 4, 33 -- From Aleksandr.Mironov-at-manchester.ac.uk Sun Jun 15 11:46:28 2008 4, 33 -- Received: from clarity.mcc.ac.uk (clarity.mcc.ac.uk [130.88.200.144]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5FGkQvo014400 4, 33 -- for {microscopy-at-microscopy.com} ; Sun, 15 Jun 2008 11:46:28 -0500 4, 33 -- Received: from webmail-w4.its.manchester.ac.uk ([10.2.61.7]) 4, 33 -- by clarity.mcc.ac.uk with esmtp (Exim 4.69 (FreeBSD)) 4, 33 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 4, 33 -- id 1K7vPi-000Fqj-P5; Sun, 15 Jun 2008 17:49:10 +0100 4, 33 -- Received: by webmail-w4.its.manchester.ac.uk (Postfix, from userid 30) 4, 33 -- id B6A031000048; Sun, 15 Jun 2008 17:49:10 +0100 (BST) 4, 33 -- Received: from cpc1-bagu7-0-0-cust534.bagu.cable.ntl.com 4, 33 -- (cpc1-bagu7-0-0-cust534.bagu.cable.ntl.com [80.2.178.23]) by 4, 33 -- webmail.manchester.ac.uk (Horde MIME library) with HTTP; Sun, 15 Jun 2008 4, 33 -- 17:49:10 +0100 4, 33 -- Message-ID: {20080615174910.89r41547b4g00gsg-at-webmail.manchester.ac.uk} 4, 33 -- X-Priority: 3 (Normal) 4, 33 -- Date: Sun, 15 Jun 2008 17:49:10 +0100 4, 33 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} 4, 33 -- To: nyilmaz-at-mersin.edu.tr 4, 33 -- Cc: microscopy-at-microscopy.com 4, 33 -- Subject: Re: [Microscopy] does staining artifact occur due to section 4, 33 -- thickness??? 4, 33 -- References: {200806141212.m5ECCc8k023438-at-ns.microscopy.com} 4, 33 -- In-Reply-To: {200806141212.m5ECCc8k023438-at-ns.microscopy.com} 4, 33 -- MIME-Version: 1.0 4, 33 -- Content-Type: text/plain; 4, 33 -- charset=UTF-8; 4, 33 -- DelSp="Yes"; 4, 33 -- format="flowed" 4, 33 -- Content-Disposition: inline 4, 33 -- Content-Transfer-Encoding: 7bit 4, 33 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.6) 4, 33 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. ==============================End of - Headers==============================
==============================Original Headers============================== 15, 25 -- From TindallR-at-missouri.edu Tue Jun 17 09:18:38 2008 15, 25 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5HEIbD8004610 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 09:18:37 -0500 15, 25 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 15, 25 -- Tue, 17 Jun 2008 09:20:03 -0500 15, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 25 -- Content-class: urn:content-classes:message 15, 25 -- MIME-Version: 1.0 15, 25 -- Content-Type: text/plain; 15, 25 -- charset="us-ascii" 15, 25 -- Subject: RE: [Microscopy] Re: does staining artifact occur due to section 15, 25 -- Date: Tue, 17 Jun 2008 09:20:03 -0500 15, 25 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7702-at-UM-XMAIL08.um.umsystem.edu} 15, 25 -- In-Reply-To: {200806151648.m5FGmeZu016098-at-ns.microscopy.com} 15, 25 -- X-MS-Has-Attach: 15, 25 -- X-MS-TNEF-Correlator: 15, 25 -- Thread-Topic: [Microscopy] Re: does staining artifact occur due to section 15, 25 -- Thread-Index: AcjPCBMPMu8bL8/YRW+M7jkkpCYQpgBfIWMg 15, 25 -- References: {200806151648.m5FGmeZu016098-at-ns.microscopy.com} 15, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 15, 25 -- To: {microscopy-at-microscopy.com} 15, 25 -- X-OriginalArrivalTime: 17 Jun 2008 14:20:03.0341 (UTC) FILETIME=[3BD9B3D0:01C8D085] 15, 25 -- Content-Transfer-Encoding: 8bit 15, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5HEIbD8004610 ==============================End of - Headers==============================
The suggestion of mercaptoethanol is quite interesting. I'm trying hard to think how breaking S-S bonds could impact precipitates.
Did you do any controlled comparisons, etc, and could you share the concentrations and way you implemented this into your protocols?
Paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-392
==============================Original Headers============================== 7, 20 -- From paul_hazelton-at-umanitoba.ca Tue Jun 17 09:38:59 2008 7, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5HEcxXT018131 7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 09:38:59 -0500 7, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 7, 20 -- (authenticated bits=0) 7, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m5HEeP3V014060; 7, 20 -- Tue, 17 Jun 2008 09:40:25 -0500 (CDT) 7, 20 -- Message-ID: {4857CCDA.8010806-at-umanitoba.ca} 7, 20 -- Date: Tue, 17 Jun 2008 09:40:26 -0500 7, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 20 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 7, 20 -- MIME-Version: 1.0 7, 20 -- To: TindallR-at-missouri.edu, Microscopy Listserver {microscopy-at-microscopy.com} 7, 20 -- Subject: Re: [Microscopy] does staining artifact occur due to section 7, 20 -- References: {200806171420.m5HEK3CB007555-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200806171420.m5HEK3CB007555-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
Yes I know, FEI do not make EDX systems, but third-party items mounted on their Tecnai FEGTEMs (which is the system I am refering to) do have to be run through their software ESVision- I know of no FEI Tecnai that does not run the EDX system through ESVision.
Believe me, I have tried using EDX systems without ESVisions intervention (on several FEGTEMs) and it is nigh-on impossible. I hope that clarifies things.
Yours, Jon
On Jun 17 2008, Straszheim, Warren E [M S E] wrote:
} Who's EDS system is it? You say it is installed on an FEI SEM, but the } last I knew, FEI does not make EDS systems. They might integrate them } with their scope for purchase, but I think they work with multiple } vendors. There should be a label on the hardware for the EDS system or on } the detector. The software might also give an indication of the brand and } version. } } Warren Straszheim } Iowa State University
==============================Original Headers============================== 5, 27 -- From jsb43-at-hermes.cam.ac.uk Tue Jun 17 11:34:24 2008 5, 27 -- Received: from ppsw-9.csi.cam.ac.uk (ppsw-9.csi.cam.ac.uk [131.111.8.139]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5HGYN9O003626 5, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 11:34:23 -0500 5, 27 -- X-Cam-SpamDetails: Not scanned 5, 27 -- X-Cam-AntiVirus: No virus found 5, 27 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 5, 27 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:55832) 5, 27 -- by ppsw-9.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.159]:25) 5, 27 -- with esmtpa (EXTERNAL:jsb43) id 1K8e9o-0005yu-TN (Exim 4.67) for Microscopy-at-microscopy.com 5, 27 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Tue, 17 Jun 2008 17:35:44 +0100 5, 27 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 5, 27 -- with local (PRAYER:jsb43) id 1K8e9o-0003CH-2w (Exim 4.67) for Microscopy-at-microscopy.com 5, 27 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Tue, 17 Jun 2008 17:35:44 +0100 5, 27 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 5, 27 -- To: MSA Listserver {Microscopy-at-microscopy.com} 5, 27 -- Subject: RE:ESVision: output of EDX quantification 5, 27 -- Date: 17 Jun 2008 17:35:43 +0100 5, 27 -- X-Mailer: Prayer v1.2.2.1 5, 27 -- X-Originating-IP: [131.111.102.18] 5, 27 -- In-Reply-To: {16A330AC32056A40B32842EC4BB8D72702B90A9E-at-maire.eng.iastate.edu} 5, 27 -- Message-ID: {Prayer.1.2.2.1.0806171735430.7984-at-hermes-1.csi.cam.ac.uk} 5, 27 -- References: {200806171405.m5HE5ENC024016-at-ns.microscopy.com} 5, 27 -- {16A330AC32056A40B32842EC4BB8D72702B90A9E-at-maire.eng.iastate.edu} 5, 27 -- Mime-Version: 1.0 5, 27 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 5, 27 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
I am just wondering what people are doing out there for low temperature polymerization of acrylic resins for immuno-EM. I have a Leica AFS I, but am finding it difficult to transfer samples and embed them when I can not see them. I have been looking at the UV Cryo Chambers from Ted Pella and EMS. Has anyone found a better system? When my samples are so small, and unosmicated, I feel like I am working blind.
Thanks,
Rhonda
==============================Original Headers============================== 8, 20 -- From rra-at-stowers-institute.org Tue Jun 17 13:32:09 2008 8, 20 -- Received: from stowers-institute.org (mail.stowers-institute.org [204.56.6.8]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5HIW8nC022242 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 13:32:09 -0500 8, 20 -- From: "Allen, Rhonda" {RRA-at-stowers-institute.org} 8, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 8, 20 -- Date: Tue, 17 Jun 2008 13:33:07 -0500 8, 20 -- Subject: Low Temp Polymerization 8, 20 -- Thread-Topic: Low Temp Polymerization 8, 20 -- Thread-Index: AcjQqJZAJruskEyhTACyE6IrUwKMVw== 8, 20 -- Message-ID: {BD62CBAC4395B94096109020651BE2EC129EC5181F-at-exchmb-02.stowers-institute.org} 8, 20 -- Accept-Language: en-US 8, 20 -- Content-Language: en-US 8, 20 -- X-MS-Has-Attach: 8, 20 -- X-MS-TNEF-Correlator: 8, 20 -- acceptlanguage: en-US 8, 20 -- Content-Type: text/plain; charset="us-ascii" 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5HIW8nC022242 ==============================End of - Headers==============================
Rhonda, I don't have experience with those products but I do have experience with small samples. I have found the use of wire loops to be of inestimable value. I make a loop with finest gauge copper wire and capture a Formvar film over it. The loop is a 3 to 6 mm diam (depending on my application) and the stem is a few cm long. I place my sample on the Formvar and for cryofixation by plunging, the sample is stuck happily ever after. I also place the sample on the loop for chemical fixation but here I need to capture a second formvar film over the first, now encasing the sample. Then being encased between Formvar films, the sample is good to go. Not only does this make it really easy to change solutions without losing the sample, I have discovered that with my tiny fragile sample, the immobilization on Formvar leads to better structural preservation. I call this "mechanical fixation" although I'll be the first to admit that a cute name is miles away from an explained phenomenon. Solvents get through Formvar and by making the loop with the finest gauge wire then I can embed the whole shmear and trim the wire with a razor. You can slice right through it.
If this sounds useful I can send you more details.
Good luck, Tobias
} } ---------------------------------------------------------------------------- } } Hello, } } I am just wondering what people are doing out there for low } temperature polymerization of acrylic resins for immuno-EM. I have } a Leica AFS I, but am finding it difficult to transfer samples and } embed them when I can not see them. I have been looking at the UV } Cryo Chambers from Ted Pella and EMS. Has anyone found a better } system? When my samples are so small, and unosmicated, I feel like } I am working blind. } } Thanks, } } Rhonda } } } }
I have an old Reichert OMU 2 or 3 ultramicrotome. To be honest, I'm not sure which model it is; I've had both in the past. It doesn't say on it, but the part number or serial number is 313 828. I want to get rid of it. I am NOT willing to pack it up and ship it anywhere (been there, done that, getting too old and cranky). But if anyone wants any easy-to-remove parts, I am willing to ship them. I also have an original Reichert service and repair kit - big brown suitcase-type with a few OLD tools and parts, like rotting motor mounts, probably only good for historical purposes.
Let me know within a week if you are interested!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 19 -- From tina-at-pbrc.hawaii.edu Tue Jun 17 20:07:44 2008 6, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5I17hd2027442 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 20:07:44 -0500 6, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m5I18mXh014118 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 15:08:49 -1000 (HST) 6, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m5I18mgA014115 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jun 2008 15:08:48 -1000 (HST) 6, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 19 -- Date: Tue, 17 Jun 2008 15:08:47 -1000 (HST) 6, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 19 -- X-Sender: tina-at-halia 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- Subject: Old Reichert OMU2 or 3 6, 19 -- Message-ID: {Pine.GSO.4.21.0806171508160.14095-100000-at-halia} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I'd like to thank you first, for your kindly efforts to help solving my precipitation problem. I'm very pleased to be a member of the group. I put some sample pictures with precipitation problem for examining on a web page its link given below. I wrote also our detailed solution preparation, processing and contrasting protocols below. Additionally, we checked our sections without contrasting and contrasting with UA only and we didn't find any problem.
Thanks in advance...
Necat Yilmaz
Link: http://electron-microscopy.blogspot.com/
PROTOCOLS:
Processing:
1- Fixation in %2.5 cold GA 4-6 hours 2- Washing in cold Phosphate Buffer 3- Post-fixation in %1 cold osmic acid 1 hour 4- Washing in cold Phosphate Buffer 5- Dehydration in graded cold alcohols 6- Propilen oxide 2X15 min 7- Propilen+resin mixture 3x30 min 8- Resin with agitation overnight 9- Embedding
Staining:
1- Floating (I don't know why?) on UA drop 5-10 min 2- Washing in carbonate free dH2O 3- Floating on lead citrate 2 min 4- Washing in carbonate free dH2O
Preparation of solutions:
UA: 1- Saturation in dH2O 2- Filtering with Whatman filter paper 3- Filtering with 0.22
Lead Citrate: 1- 1.33 gr Lead nitrate 2- 1.76 sodium citrate 3- Mix in 30 ml carbonate free dH2O for 30 min 4- Add 8 ml 1 N NaOH 5- Add 12 ml carbonate free dH2O
==============================Original Headers============================== 14, 32 -- From nyilmaz-at-mersin.edu.tr Wed Jun 18 03:35:58 2008 14, 32 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5I8Zmlk020793 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 03:35:56 -0500 14, 32 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 14, 32 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 9461E2244A4 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 11:34:42 +0300 (EEST) 14, 32 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 14, 32 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 14, 32 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 14, 32 -- with ESMTP id FNoBSKViy5NK for {Microscopy-at-microscopy.com} ; 14, 32 -- Wed, 18 Jun 2008 11:34:12 +0300 (EEST) 14, 32 -- Received: from nejat (unknown [193.255.129.131]) 14, 32 -- by mail.mersin.edu.tr (Postfix) with SMTP id 0B99B2244F4 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 11:34:03 +0300 (EEST) 14, 32 -- Message-ID: {002701c8d11e$587d7460$6b01a8c0-at-nejat} 14, 32 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 14, 32 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 14, 32 -- Subject: does staining artifact occur due to section thickness-2 14, 32 -- Date: Wed, 18 Jun 2008 11:35:50 +0300 14, 32 -- MIME-Version: 1.0 14, 32 -- Content-Type: text/plain; 14, 32 -- format=flowed; 14, 32 -- charset="iso-8859-9"; 14, 32 -- reply-type=original 14, 32 -- Content-Transfer-Encoding: 7bit 14, 32 -- X-Priority: 3 14, 32 -- X-MSMail-Priority: Normal 14, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 14, 32 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 14, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 14, 32 -- X-Virus-Scanned: ClamAV using ClamSMTP ==============================End of - Headers==============================
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Email: bjulies-at-uwc.ac.za Name: Basil Julies
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Title-Subject: [Filtered] Post in South Africa : EM operator for tecnai F20 TEM on senior lecturer level
Question: Hi I hereby repeat an advertisement for a tecnai F20 FEG TEM operator post on a senior lecturer level at the University of the Western Cape, South Africa. This is therefore an academic post, giving the incumbent access to all the benefits which academics enjoy. The incumbent must have a PhD or equivalent degree, with experience in materials science and experience in employing/interpreting and analysing HR STEM/TEM and (especially) EELS. The appointment is for 1 January 2009, and the deadline is 31 July 2008. Any questions can be directed to me directly. The full advertisement can be forwarded to interested parties once contact has been made with me.
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Email: rharding-at-emsl.com Name: Richard Harding
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Title-Subject: [Filtered] TEM
Question: We are interested in purchasing used JEOL TEM 100 CXII microscopes. If you have a TEM or know of one that might be available, please contact me.
I would like to extend my gratitude to the microscopy list server community for all of the replies I received regarding my SEM upgrade. It was very helpful to have users opinions, advice, leads to helpful individual within suppliers where the normal entry route failed and pointing me in directions where I might not have ventured.
While there may be minor issues, to the credit of the suppliers of these packages, all replies I received very positive with regard to quality, reliability and support.
Alan Stone ASTON
==============================Original Headers============================== 6, 19 -- From as-at-astonmet.com Wed Jun 18 09:03:27 2008 6, 19 -- Received: from outbound3.mail.tds.net (outbound3.mail.tds.net [216.170.230.93]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IE3Qd3020948 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 09:03:26 -0500 6, 19 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32]) 6, 19 -- by outbound3.mail.tds.net (8.13.6/8.13.4) with ESMTP id m5IE45EE021997 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 09:04:05 -0500 6, 19 -- Received: from mozart.astonmet.com ([69.11.219.4]) 6, 19 -- by outaamta02.mail.tds.net with ESMTP 6, 19 -- id {20080618140404.IIBW4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} 6, 19 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 09:04:04 -0500 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 19 -- Date: Wed, 18 Jun 2008 09:04:03 -0500 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- From: Alan Stone {as-at-astonmet.com} 6, 19 -- Subject: SEM Upgrade--Thank You 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- Message-Id: {20080618140404.IIBW4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} ==============================End of - Headers==============================
At least one of the images looks similar to a problem that I had (and occasionally still have).
Have you looked at an unstained section on the TEM? Can you see spots?
I found that some of the spots were there before staining. The problem seems to come from the air. When I work fast to pick up the sections from the boat and limit the air moving around by closing off adjacent doors to the room that my microtome is located in and put a filter over the room air source, I can get large areas that are clean.
At times when I get interrupted during my sectioning I can actually see that things have landed on the surface of my knife boat water and the sections that I pick up are dirty. I now start over using fresh water. If I remember correctly, you have recently started using a diamond knife. The boat is so much larger so you are most likely sectioning for a longer time than you had when using glass knives.
My water is acidic (not distilled) so I have started to use basic water (1 pellet of NaOH to a pint bottle of water) for the drop that I put the grid into (rather than on) before the lead stain step in my staining protocol. Maybe I imagine that it gives me better results but it seems to be working well.
Better luck, Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {nyilmaz-at-mersin.edu.tr} } Reply-To: {nyilmaz-at-mersin.edu.tr} } Date: Wed, 18 Jun 2008 03:41:44 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] does staining artifact occur due to section thickness-2 } } Hello again, } } I'd like to thank you first, for your kindly efforts to help solving my } precipitation problem. I'm very pleased to be a member of the group. } I put some sample pictures with precipitation problem for examining on a web } page its link given below. I wrote also our detailed solution preparation, } processing and contrasting protocols below. Additionally, we checked our } sections without contrasting and contrasting with UA only and we didn't find } any problem. } } Thanks in advance... } } Necat Yilmaz } } Link: http://electron-microscopy.blogspot.com/ } } PROTOCOLS: } } Processing: } } 1- Fixation in %2.5 cold GA 4-6 hours } 2- Washing in cold Phosphate Buffer } 3- Post-fixation in %1 cold osmic acid 1 hour } 4- Washing in cold Phosphate Buffer } 5- Dehydration in graded cold alcohols } 6- Propilen oxide 2X15 min } 7- Propilen+resin mixture 3x30 min } 8- Resin with agitation overnight } 9- Embedding } } Staining: } } 1- Floating (I don't know why?) on UA drop 5-10 min } 2- Washing in carbonate free dH2O } 3- Floating on lead citrate 2 min } 4- Washing in carbonate free dH2O } } Preparation of solutions: } } UA: } 1- Saturation in dH2O } 2- Filtering with Whatman filter paper } 3- Filtering with 0.22 } } Lead Citrate: } 1- 1.33 gr Lead nitrate } 2- 1.76 sodium citrate } 3- Mix in 30 ml carbonate free dH2O for 30 min } 4- Add 8 ml 1 N NaOH } 5- Add 12 ml carbonate free dH2O
} ==============================End of - Headers==============================
==============================Original Headers============================== 13, 29 -- From connellyps-at-nhlbi.nih.gov Wed Jun 18 09:40:18 2008 13, 29 -- Received: from nihxway6out.hub.nih.gov (nihxway6out.hub.nih.gov [128.231.90.114]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IEeI5s002593 13, 29 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 09:40:18 -0500 13, 29 -- X-IronPortListener: Outbound_SMTP 13, 29 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) 13, 29 -- by nihxway6out.hub.nih.gov with ESMTP; 18 Jun 2008 10:40:59 -0400 13, 29 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 13, 29 -- Wed, 18 Jun 2008 10:40:53 -0400 13, 29 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.168]) with Microsoft Exchange Server HTTP-DAV ; 13, 29 -- Wed, 18 Jun 2008 14:40:52 +0000 13, 29 -- User-Agent: Microsoft-Entourage/11.4.0.080122 13, 29 -- Date: Wed, 18 Jun 2008 10:37:06 -0400 13, 29 -- Subject: Re: [Microscopy] does staining artifact occur due to section 13, 29 -- thickness-2 13, 29 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 13, 29 -- To: {nyilmaz-at-mersin.edu.tr} 13, 29 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 13, 29 -- Message-ID: {C47E95D2.1ED3%connellyps-at-nhlbi.nih.gov} 13, 29 -- Thread-Topic: [Microscopy] does staining artifact occur due to section 13, 29 -- thickness-2 13, 29 -- Thread-Index: AcjRUMfQBqKMBj1EEd2GRAANk2Yv1A== 13, 29 -- In-Reply-To: {200806180841.m5I8fijE028579-at-ns.microscopy.com} 13, 29 -- Mime-version: 1.0 13, 29 -- Content-type: text/plain; 13, 29 -- charset="ISO-8859-1" 13, 29 -- X-OriginalArrivalTime: 18 Jun 2008 14:40:53.0501 (UTC) FILETIME=[4F6ABAD0:01C8D151] 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5IEeI5s002593 ==============================End of - Headers==============================
Hello all, Just a few moments ago, I have noticed that temperature sensor of our MegaViewII CCD camera shows really high temperature of CCD Chip (25,5 oC = 77.9 F ) and Housing (30 oC = 86 F ).
Has anybody any suggestion or hint?
Thanking you in advance. Oldrich
P.S. Screen capture of information panel can be seen on: http://www2.biomed.cas.cz/~benada/MGV/index.html
------------------------------------------ Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 6, 21 -- From benada-at-biomed.cas.cz Wed Jun 18 10:18:29 2008 6, 21 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IFISBV017097 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 10:18:29 -0500 6, 21 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 6, 21 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 92CA41A443FA 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 17:19:08 +0200 (CEST) 6, 21 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 6, 21 -- Organization: Institute of Microbiology 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Date: Wed, 18 Jun 2008 17:19:06 +0200 6, 21 -- MIME-Version: 1.0 6, 21 -- Subject: MegaViewII Peltier temperature 6, 21 -- Message-ID: {4859438A.21849.20EF2AE-at-benada.biomed.cas.cz} 6, 21 -- Priority: normal 6, 21 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 21 -- Content-type: text/plain; charset=US-ASCII 6, 21 -- Content-transfer-encoding: 7BIT 6, 21 -- Content-description: Mail message body 6, 21 -- X-Antivirus: avast! (VPS 080617-0, 17.06.2008), Outbound message 6, 21 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
} Good Afternoon, } } Thank you for all your terrific feedback re: Service Engineers for } Leica Microtomes.
The two most popular recommendations are:
MOC (Microscopical Optical Consulting). MOC is located in upstate NY. Address: MOC, PO Box 586, Valley Cottage, NY 10989 Contact: Helmut Patzig Phone: 845-268-6450 Fax: 845-268-0172 Email: MOCLeica-at-aol.com
and
} John Petz and Tek-Net Inc, Lakewood NJ, See http://www.teknetinc.com/2682/index.html
Another question.. even though we are trying to hold onto our Ultracut E's.. the day may come when we need to convince "the powers that be" to buy a new microtome.
What is your impression of the newest and latest Leica Ultramicrotome (UC6).. for normal everyday epoxy sectioning of biological samples? Serial Sectioning? Ease of use? etc..
Are there any reliable ultramicrotome repair people/companies on the west coast? 2 good ones on the east coast, I'm jealous!
I'm going to be throwing away some 35mm bulk loaders and the little spools you load film onto unless someone wants them. Some of the bulk loaders have film in them, though the film is very old and was stored in a drawer not refrigerated.
You'll have to pay for shipping. You know the Feds, we nickel and dime you to death ;)
Paula :-)
Paula Sicurello VA Hospital San Diego Microscope Facility room B141 3350 La Jolla Village Drive San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 9, 24 -- From vapatpxs-at-yahoo.com Wed Jun 18 13:45:20 2008 9, 24 -- Received: from n64a.bullet.mail.sp1.yahoo.com (n64a.bullet.mail.sp1.yahoo.com [98.136.45.11]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5IIjK2w030927 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 13:45:20 -0500 9, 24 -- Received: from [216.252.122.217] by n64.bullet.mail.sp1.yahoo.com with NNFMP; 18 Jun 2008 18:45:53 -0000 9, 24 -- Received: from [69.147.65.155] by t2.bullet.sp1.yahoo.com with NNFMP; 18 Jun 2008 18:45:53 -0000 9, 24 -- Received: from [127.0.0.1] by omp403.mail.sp1.yahoo.com with NNFMP; 18 Jun 2008 18:45:53 -0000 9, 24 -- X-Yahoo-Newman-Property: ymail-3 9, 24 -- X-Yahoo-Newman-Id: 803310.52019.bm-at-omp403.mail.sp1.yahoo.com 9, 24 -- Received: (qmail 55398 invoked by uid 60001); 18 Jun 2008 18:45:53 -0000 9, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 24 -- s=s1024; d=yahoo.com; 9, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 9, 24 -- b=i0mDUohjhQ+0NnDYuQL9h746n5iBqJ6gt3mlG81dHpgopQsdalDsPXa4MfQmYWxxFirvS0af2lYtUHdp4m5VUwVTUyfOQ3WOUI2f5TaZ1CB8lNs2jgIZLsDICNQS5TcJOqwut7fq11B0Td7Asi/sLbnfKRc0FFwStu6ozIc5/9I=; 9, 24 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com via HTTP; Wed, 18 Jun 2008 11:45:53 PDT 9, 24 -- X-Mailer: YahooMailWebService/0.7.199 9, 24 -- Date: Wed, 18 Jun 2008 11:45:53 -0700 (PDT) 9, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 9, 24 -- Reply-To: vapatpxs-at-yahoo.com 9, 24 -- Subject: Service for Ultramicrotomes, West Coast? 9, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 9, 24 -- MIME-Version: 1.0 9, 24 -- Content-Type: text/plain; charset=us-ascii 9, 24 -- Message-ID: {599596.53419.qm-at-web46108.mail.sp1.yahoo.com} ==============================End of - Headers==============================
Patricia Connelly (see extract, below) makes some good points: some contamination appears to come from the air. Over the years, I've encountered this problem in labs where rotary pumps were not properly exhausted and microdroplets of oil vapor are present in the air. In one instance, the pump exhaust was attached to a plastic pipe that disappeared into the ceiling, supposedly to be vented outside. Guess what? The pipe terminated in the space above the false ceiling. Likewise, a fume hood that was supposed to exhaust to the outside had a broken vent pipe that was doing the same. Now, such events would result in a major OSHA investigation and lab shutdown, probably.
Other sources for air-borne contamination: drive belts from electric motors (notorious for giving off tiny, carbonaceous particles), chemicals on shelves that give off volatiles (ammonium compounds, organic buffers, HCl, for example). Often, the chemical volatiles combine to form fine powders (like NH4Cl). Store these chemical in a properly vented fume hood.
Contamination may also be introduced via the water used for staining and sectioning. Some examples: volatile amines from house distilled water, lubricants used on syringe plungers, humectants used on micropore filters, decomposing micropore filters (due to using old filters or by bacterial degradation of filters that have not been changed), oily contamination in diamond knife boats that have never been cleaned, oily contamination from eyelash probes, improperly cleaned glassware.
It's a dirty world out there!
JB
EXTRACT OF MESSAGE:
I found that some of the spots were there before staining. The problem seems to come from the air. When I work fast to pick up the sections from the boat and limit the air moving around by closing off adjacent doors to the room that my microtome is located in and put a filter over the room air source, I can get large areas that are clean.
At times when I get interrupted during my sectioning I can actually see that things have landed on the surface of my knife boat water and the sections that I pick up are dirty. I now start over using fresh water. If I remember correctly, you have recently started using a diamond knife. The boat is so much larger so you are most likely sectioning for a longer time than you had when using glass knives.
My water is acidic (not distilled) so I have started to use basic water (1 pellet of NaOH to a pint bottle of water) for the drop that I put the grid into (rather than on) before the lead stain step in my staining protocol. Maybe I imagine that it gives me better results but it seems to be working well.
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
A few years ago I got a UC6. It is wonderful. I cut extensive serial sections from epoxy blocks. The sections are very similar in thickness (better than any other microtome I have used). I will regularly put 100+ sections on a grid and the reproducibility is, well, reproducible :-) Any way, contact me if you want to ask specific questions.
Just a satisfied customer.
David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
On Jun 18, 2008, at 11:22 AM, crockerv-at-ninds.nih.gov wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good Afternoon, } } Another question.. even though we are trying to hold onto our } Ultracut E's.. the day may come when we need to convince "the powers } that be" to buy a new microtome. } } What is your impression of the newest and latest Leica Ultramicrotome } (UC6).. for normal everyday epoxy sectioning of biological samples? } Serial Sectioning? Ease of use? etc.. } } Thank you, } Virginia } } } -- } Virginia Tanner Crocker } Biologist } NIH, NINDS EM Facility } MSC4477 } Bldg. 49, Room 3C58 } 49 Convent Drive } Bethesda, MD 20892 } 301-402-1568 } 301-480-1485 (fax) } crockerv-at-ninds.nih.gov (email) } }
==============================Original Headers============================== 14, 21 -- From Elliott-at-arizona.edu Wed Jun 18 13:50:15 2008 14, 21 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 14, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IIoEYs012466 14, 21 -- for {MICROSCOPY-at-ns.microscopy.com} ; Wed, 18 Jun 2008 13:50:15 -0500 14, 21 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 14, 21 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 31410652771 14, 21 -- for {MICROSCOPY-at-ns.microscopy.com} ; Wed, 18 Jun 2008 11:50:48 -0700 (MST) 14, 21 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 14, 21 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id C3C6E65791A 14, 21 -- for {MICROSCOPY-at-ns.microscopy.com} ; Wed, 18 Jun 2008 11:50:46 -0700 (MST) 14, 21 -- Message-Id: {B60724DD-BE0A-419A-906A-CE9B1AFE94E9-at-arizona.edu} 14, 21 -- From: David Elliott {Elliott-at-arizona.edu} 14, 21 -- To: MICROSCOPY-at-ns.microscopy.com 14, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 14, 21 -- Content-Transfer-Encoding: 7bit 14, 21 -- Mime-Version: 1.0 (Apple Message framework v924) 14, 21 -- Subject: Re: [Microscopy] New Ultracut Microtomes 14, 21 -- Date: Wed, 18 Jun 2008 11:50:45 -0700 14, 21 -- References: {500B20CA-DA61-426E-9DFA-7113CD917ABA-at-arizona.edu} 14, 21 -- X-Mailer: Apple Mail (2.924) 14, 21 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Yikes, that some bad dirt! It does look like something other than the staining protocol, so check and clean your knife (including the boat and the backside of the diamond), your eyelash and forceps and any other tools you use, fill the boat with your best water from a syringe with a new, clean syringe filter, etc.
For staining with lead, I always stain within a closed petri dish with several (or a small pile of) pellets of NaOH on the side to further reduce carbonate. This seems essential in my lab to reduce the really fine pepper. Test your "carbonate-free water" by dipping an unstained grid in it and letting it dry.
Aloha, Tina
} I'd like to thank you first, for your kindly efforts to help solving my } precipitation problem. I'm very pleased to be a member of the group. } I put some sample pictures with precipitation problem for examining on a web } page its link given below. I wrote also our detailed solution preparation, } processing and contrasting protocols below. Additionally, we checked our } sections without contrasting and contrasting with UA only and we didn't find } any problem. } } Thanks in advance... } } Necat Yilmaz } } Link: http://electron-microscopy.blogspot.com/ } } PROTOCOLS: } } Processing: } } 1- Fixation in %2.5 cold GA 4-6 hours } 2- Washing in cold Phosphate Buffer } 3- Post-fixation in %1 cold osmic acid 1 hour } 4- Washing in cold Phosphate Buffer } 5- Dehydration in graded cold alcohols } 6- Propilen oxide 2X15 min } 7- Propilen+resin mixture 3x30 min } 8- Resin with agitation overnight } 9- Embedding } } Staining: } } 1- Floating (I don't know why?) on UA drop 5-10 min } 2- Washing in carbonate free dH2O } 3- Floating on lead citrate 2 min } 4- Washing in carbonate free dH2O } } Preparation of solutions: } } UA: } 1- Saturation in dH2O } 2- Filtering with Whatman filter paper } 3- Filtering with 0.22 } } Lead Citrate: } 1- 1.33 gr Lead nitrate } 2- 1.76 sodium citrate } 3- Mix in 30 ml carbonate free dH2O for 30 min } 4- Add 8 ml 1 N NaOH } 5- Add 12 ml carbonate free dH2O } } } ==============================Original Headers============================== } 14, 32 -- From nyilmaz-at-mersin.edu.tr Wed Jun 18 03:35:58 2008 } 14, 32 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) } 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5I8Zmlk020793 } 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 03:35:56 -0500 } 14, 32 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) } 14, 32 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 9461E2244A4 } 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 11:34:42 +0300 (EEST) } 14, 32 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr } 14, 32 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) } 14, 32 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) } 14, 32 -- with ESMTP id FNoBSKViy5NK for {Microscopy-at-microscopy.com} ; } 14, 32 -- Wed, 18 Jun 2008 11:34:12 +0300 (EEST) } 14, 32 -- Received: from nejat (unknown [193.255.129.131]) } 14, 32 -- by mail.mersin.edu.tr (Postfix) with SMTP id 0B99B2244F4 } 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 11:34:03 +0300 (EEST) } 14, 32 -- Message-ID: {002701c8d11e$587d7460$6b01a8c0-at-nejat} } 14, 32 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} } 14, 32 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} } 14, 32 -- Subject: does staining artifact occur due to section thickness-2 } 14, 32 -- Date: Wed, 18 Jun 2008 11:35:50 +0300 } 14, 32 -- MIME-Version: 1.0 } 14, 32 -- Content-Type: text/plain; } 14, 32 -- format=flowed; } 14, 32 -- charset="iso-8859-9"; } 14, 32 -- reply-type=original } 14, 32 -- Content-Transfer-Encoding: 7bit } 14, 32 -- X-Priority: 3 } 14, 32 -- X-MSMail-Priority: Normal } 14, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 } 14, 32 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} } 14, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 14, 32 -- X-Virus-Scanned: ClamAV using ClamSMTP } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 22 -- From tina-at-pbrc.hawaii.edu Wed Jun 18 14:23:30 2008 6, 22 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IJNTYL005565 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 14:23:30 -0500 6, 22 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 22 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m5IJNvBr018039 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 22 -- Wed, 18 Jun 2008 09:23:58 -1000 (HST) 6, 22 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m5IJNsig018035; 6, 22 -- Wed, 18 Jun 2008 09:23:57 -1000 (HST) 6, 22 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 22 -- Date: Wed, 18 Jun 2008 09:23:53 -1000 (HST) 6, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 22 -- X-Sender: tina-at-halia 6, 22 -- To: nyilmaz-at-mersin.edu.tr 6, 22 -- cc: microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] does staining artifact occur due to section 6, 22 -- thickness-2 6, 22 -- In-Reply-To: {200806180837.m5I8bf4s022376-at-ns.microscopy.com} 6, 22 -- Message-ID: {Pine.GSO.4.21.0806180906510.17854-100000-at-halia} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
We have had excellent service recently from Paul Swerdlick - Swerdlick Systems, 26852 N. Claudette St. Santa Clarita, CA 91351. Phone: 805-341-5056 Email swerdlicksystm-at-aol.com Lisa
Lisa M. Baird, Ph.D. Professor of Biology Shiley Center for Science and Technology University of San Diego 5998 Alcala Park San Diego, CA 92110 619-260-4073 (voice) 619-260-6804 (fax)
==============================Original Headers============================== 4, 23 -- From baird-at-sandiego.edu Wed Jun 18 15:00:16 2008 4, 23 -- Received: from omr1.sandiego.edu (omr1-ext.sandiego.edu [192.195.154.246]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IK0GK1019908 4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 15:00:16 -0500 4, 23 -- Received: from ST481-BRY0DB1.sandiego.edu ([10.120.99.216]) 4, 23 -- by omr1.sandiego.edu (MOS 3.8.7a) 4, 23 -- with ESMTP id ALB85298 (AUTH baird); 4, 23 -- Wed, 18 Jun 2008 13:00:47 -0700 (PDT) 4, 23 -- Message-Id: {200806182000.ALB85298-at-omr1.sandiego.edu} 4, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 4, 23 -- Date: Wed, 18 Jun 2008 13:00:47 -0700 4, 23 -- To: microscopy-at-microscopy.com 4, 23 -- From: Lisa Baird {baird-at-sandiego.edu} 4, 23 -- Subject: West Coast ultramicrotome repair 4, 23 -- Mime-Version: 1.0 4, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 23 -- X-Junkmail-Status: score=10/50, host=omr1.sandiego.edu 4, 23 -- X-Junkmail-SD-Raw: score=unknown, 4, 23 -- refid=str=0001.0A010207.4859696F.00E4,ss=1,fgs=0, 4, 23 -- ip=10.120.99.216, 4, 23 -- so=2008-05-01 23:44:25, 4, 23 -- dmn=5.4.3/2008-02-01 4, 23 -- X-Junkmail-IWF: false ==============================End of - Headers==============================
Thanks for posting the pictures. This does look like lead precipitation.
First of all, answering your original question - although there is hardly a direct relation to thickness, sections of lesser quality do get dirty much easier. Second picture looks like this could be in issue there. And thinner sections are *definitely* more likely to come out in lower quality. Did you try cutting thicker on the diamond? For all except really resolution-demanding work, I personally prefer sections that are just a little bit yellowish, silver is too thin and will be more difficult to stain.
I would first of all experiment switching back and forth, glass to diamond an back, also varying the thickness. Will it still be that thinner sections get dirty? Then it is the lower quality sections "trapping" the lead. There is also a possibility, of course, that your new diamond knife setup got dirty somehow - the boat? edge?..
From your protocol, I see that you do use CO2-free water already. I will just add that it is also important (from my own tests, long ago) to have your NaOH as CO2-free as possible. I routinely got Pb contamination from old, long since opened, NaOH granules. Here we now use titrated 1.0 N "CO2-free" NaOH solution sold by a company, but before that was available, I would keep a special bottle of NaOH granules, for EM lead stain only, and replace it with a freshly opened one regularly. From my experience, this was more important than the extra water you add to make the stain.
Dipping instead of floating, mentioned by others, is another measure apparently making a difference. One more thing, you can surround your grids being stained by granules of NaOH - set this arrangement on a lid of Petri dish and cover with the bottom. Finally, you can try rinsing the grids after staining with water that has some NaOH in it - 0.01-0.02 N. I never had to resort to this, though, and there is, of course, a flip side to it - your staining may become weaker. Just like if the pH of your Reynolds is too high.
Although this is not directly what you are asking, may I suggest a couple more tips to your protocol? They should help with better structural preservation and, likely, better sections: 1) 4-5% GA, instead of 2.5%. (2-4 hours should be enough). 2) during dehydration, include a step of 1.5% UA in 70% ethanol, overnight in the fridge. Then you can skip UA staining of sections. 3) from the pictures, you seem to have some infiltration issues. Consider modifying your infiltration schedule. A lot will depend on how you actually handle things, fluid changes and all, but you may be going through your PO/resin mixtures too fast - 3x30 min? Try leaving the samples in one of the mixtures, 1:1, or, better, 1PO:3epoxy overnight, and in other mixtures for ~2 h. Then, finally, 2-4 h (less critical) in pure resin, then into embedding molds with fresh resin and right into 60degC oven. Catalized epoxy thickens quickly, so it is a good idea to make it without the catalyst (BDMA? DMP30?) and add it to one portion before making mixtures for infiltration and to another portion the next day, to have the resin fresh and less viscous.
Good luck, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} From: nyilmaz-at-mersin.edu.tr } Date: June 18, 2008 4:37:36 AM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] does staining artifact occur due to section } thickness-2 } Reply-To: nyilmaz-at-mersin.edu.tr } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello again, } } I'd like to thank you first, for your kindly efforts to help } solving my } precipitation problem. I'm very pleased to be a member of the group. } I put some sample pictures with precipitation problem for examining } on a web } page its link given below. I wrote also our detailed solution } preparation, } processing and contrasting protocols below. Additionally, we } checked our } sections without contrasting and contrasting with UA only and we } didn't find } any problem. } } Thanks in advance... } } Necat Yilmaz } } Link: http://electron-microscopy.blogspot.com/ } } PROTOCOLS: } } Processing: } } 1- Fixation in %2.5 cold GA 4-6 hours } 2- Washing in cold Phosphate Buffer } 3- Post-fixation in %1 cold osmic acid 1 hour } 4- Washing in cold Phosphate Buffer } 5- Dehydration in graded cold alcohols } 6- Propilen oxide 2X15 min } 7- Propilen+resin mixture 3x30 min } 8- Resin with agitation overnight } 9- Embedding } } Staining: } } 1- Floating (I don't know why?) on UA drop 5-10 min } 2- Washing in carbonate free dH2O } 3- Floating on lead citrate 2 min } 4- Washing in carbonate free dH2O } } Preparation of solutions: } } UA: } 1- Saturation in dH2O } 2- Filtering with Whatman filter paper } 3- Filtering with 0.22 } } Lead Citrate: } 1- 1.33 gr Lead nitrate } 2- 1.76 sodium citrate } 3- Mix in 30 ml carbonate free dH2O for 30 min } 4- Add 8 ml 1 N NaOH } 5- Add 12 ml carbonate free dH2O } } } ==============================Original } Headers============================== } 14, 32 -- From nyilmaz-at-mersin.edu.tr Wed Jun 18 03:35:58 2008 } 14, 32 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) } 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m5I8Zmlk020793 } 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 } 03:35:56 -0500 } 14, 32 -- Received: from localhost (localhost.mersin.edu.tr } [127.0.0.1]) } 14, 32 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 9461E2244A4 } 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 } 11:34:42 +0300 (EEST) } 14, 32 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr } 14, 32 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) } 14, 32 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) } (amavisd-new, port 10024) } 14, 32 -- with ESMTP id FNoBSKViy5NK for {Microscopy-at-microscopy.com} ; } 14, 32 -- Wed, 18 Jun 2008 11:34:12 +0300 (EEST) } 14, 32 -- Received: from nejat (unknown [193.255.129.131]) } 14, 32 -- by mail.mersin.edu.tr (Postfix) with SMTP id 0B99B2244F4 } 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 } 11:34:03 +0300 (EEST) } 14, 32 -- Message-ID: {002701c8d11e$587d7460$6b01a8c0-at-nejat} } 14, 32 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} } 14, 32 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} } 14, 32 -- Subject: does staining artifact occur due to section } thickness-2 } 14, 32 -- Date: Wed, 18 Jun 2008 11:35:50 +0300 } 14, 32 -- MIME-Version: 1.0 } 14, 32 -- Content-Type: text/plain; } 14, 32 -- format=flowed; } 14, 32 -- charset="iso-8859-9"; } 14, 32 -- reply-type=original } 14, 32 -- Content-Transfer-Encoding: 7bit } 14, 32 -- X-Priority: 3 } 14, 32 -- X-MSMail-Priority: Normal } 14, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 } 14, 32 -- Disposition-Notification-To: "Nejat Yilmaz" } {nyilmaz-at-mersin.edu.tr} } 14, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 14, 32 -- X-Virus-Scanned: ClamAV using ClamSMTP } ==============================End of - } Headers==============================
==============================Original Headers============================== 12, 23 -- From vladislav_speransky-at-nih.gov Wed Jun 18 15:01:30 2008 12, 23 -- Received: from nihrelayxway5.hub.nih.gov (nihrelayxway5.hub.nih.gov [128.231.90.99]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IK1U1M022320 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 15:01:30 -0500 12, 23 -- X-IronPortListener: NIH_Relay 12, 23 -- X-SBRS: None 12, 23 -- X-IronPort-AV: E=Sophos;i="4.27,667,1204520400"; 12, 23 -- d="scan'208";a="182178753" 12, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 12, 23 -- by nihrelayxway5.hub.nih.gov with ESMTP; 18 Jun 2008 16:02:01 -0400 12, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 12, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m5IK20ZU012225 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 16:02:00 -0400 12, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 12, 23 -- References: {200806180837.m5I8baaQ022230-at-ns.microscopy.com} 12, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 23 -- Message-Id: {11EFBD26-07F8-45CA-874F-42C4AC183915-at-nih.gov} 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 12, 23 -- Subject: Fwd: [Microscopy] does staining artifact occur due to section thickness-2 12, 23 -- Date: Wed, 18 Jun 2008 16:01:30 -0400 12, 23 -- To: Microscopy-at-microscopy.com 12, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Hello Everyone, I'm looking at a couple of emulsions (oil in water), food products like mayonaisse. What I am seeing is that the aged sample has a water structure between the oil drops that is very different from a more freshly made sample. I was wondering if anyone has experience with cryo freeze fracture SEM of food emulsions and could take a look at two images and comment on the differences? The composition is water, oil, Xanthan Gum, eggs, and a few other ingredients. Let me know your email and I will forward the two images as attachments.
Thanks!
==============================Original Headers============================== 2, 34 -- From doc.vrdoljak-at-gmail.com Wed Jun 18 17:32:41 2008 2, 34 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.180]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5IMWfZb026542 2, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 17:32:41 -0500 2, 34 -- Received: by wa-out-1112.google.com with SMTP id j5so365244wah.4 2, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 15:33:07 -0700 (PDT) 2, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 34 -- d=gmail.com; s=gamma; 2, 34 -- h=domainkey-signature:received:received:message-id:date:from:to 2, 34 -- :subject:mime-version:content-type:content-transfer-encoding 2, 34 -- :content-disposition; 2, 34 -- bh=lG0u/pj36Y6TcsbQkPEinORIgXlo7iNVgnhpYplNSXM=; 2, 34 -- b=m0pjLIyy+0qKf2XRiNZ02PuSP0lnlWn12rkkpYxzVJrWfcpJ7me9gu0cA90x689FEY 2, 34 -- OTrdo4B7GfBoFhvYQsLtR02SQDEdZmYeqninTKcWyPYfBwQbBgyF5d2me3uLei5OI6MB 2, 34 -- oUjyw84hzgCi6dNntlKq5wca/pkkMwsK0oWhk= 2, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 34 -- d=gmail.com; s=gamma; 2, 34 -- h=message-id:date:from:to:subject:mime-version:content-type 2, 34 -- :content-transfer-encoding:content-disposition; 2, 34 -- b=WAulJ9BICdmn2zDatWb9LbG2bX2JfcqBF6DvC14iiVB4aFbmh1etDOo0PfcqaV7y+F 2, 34 -- oOaonSQybxl5oqgNIvsX6jiOMkERK51mQhI+VZmmG1DcTqOAND5Ph2oYSirzXTBSacdr 2, 34 -- 3mDjTVFYF/eHKY6a9IcBIUlOvaeUh9SGGmu+E= 2, 34 -- Received: by 10.114.144.1 with SMTP id r1mr1634202wad.136.1213828387271; 2, 34 -- Wed, 18 Jun 2008 15:33:07 -0700 (PDT) 2, 34 -- Received: by 10.114.76.3 with HTTP; Wed, 18 Jun 2008 15:33:07 -0700 (PDT) 2, 34 -- Message-ID: {fb272960806181533y3bae247an750a115978767dbc-at-mail.gmail.com} 2, 34 -- Date: Wed, 18 Jun 2008 15:33:07 -0700 2, 34 -- From: "Gordon Vrdoljak" {doc.vrdoljak-at-gmail.com} 2, 34 -- To: Microscopy-at-microscopy.com 2, 34 -- Subject: cryo FF SEM of food emulsion 2, 34 -- MIME-Version: 1.0 2, 34 -- Content-Type: text/plain; charset=ISO-8859-1 2, 34 -- Content-Transfer-Encoding: 7bit 2, 34 -- Content-Disposition: inline ==============================End of - Headers==============================
It is my sad duty to report that Ralph Knowles has passed away during a recent trip to Europe.
As many of you will know, Ralph was a leading light in the development of ESEM technology, amongst his many other acheivements in electron microscopy. He will be sorely missed by many, both for his expertise and his friendship.
Best Wishes,
Debbie
==============================Original Headers============================== 5, 24 -- From djs49-at-hermes.cam.ac.uk Thu Jun 19 11:34:44 2008 5, 24 -- Received: from ppsw-5.csi.cam.ac.uk (ppsw-5.csi.cam.ac.uk [131.111.8.135]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5JGYhUU009612 5, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 19 Jun 2008 11:34:44 -0500 5, 24 -- X-Cam-SpamDetails: Not scanned 5, 24 -- X-Cam-AntiVirus: No virus found 5, 24 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 5, 24 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:45195) 5, 24 -- by ppsw-5.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.155]:25) 5, 24 -- with esmtpa (EXTERNAL:djs49) id 1K9N5m-0006K3-Gw (Exim 4.67) for Microscopy-at-microscopy.com 5, 24 -- (return-path {djs49-at-hermes.cam.ac.uk} ); Thu, 19 Jun 2008 17:34:34 +0100 5, 24 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 5, 24 -- with local (PRAYER:djs49) id 1K9N5m-0000V2-7l (Exim 4.67) for Microscopy-at-microscopy.com 5, 24 -- (return-path {djs49-at-hermes.cam.ac.uk} ); Thu, 19 Jun 2008 17:34:34 +0100 5, 24 -- From: Dr Debbie Stokes {djs49-at-cam.ac.uk} 5, 24 -- To: Microscopy-at-microscopy.com 5, 24 -- Subject: Ralph Knowles 5, 24 -- Date: 19 Jun 2008 17:34:34 +0100 5, 24 -- X-Mailer: Prayer v1.2.2.1 5, 24 -- X-Originating-IP: [88.128.81.178] 5, 24 -- Message-ID: {Prayer.1.2.2.1.0806191734340.32394-at-hermes-1.csi.cam.ac.uk} 5, 24 -- Mime-Version: 1.0 5, 24 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 5, 24 -- Sender: Dr Debbie Stokes {djs49-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
Some of the newer EVO units will work with LaB6. I can't find details about EVO 40.
LaB6 usually requires an ion pumped gun chamber. At poorer vacuums, the LaB6 cathode won't last very long. The other issue is whether the W filament holder will also hold LaB6. Earlier SEMS that could do W and LaB6 came with two different Whenelt apertures on separate/different gun holders.
It seems to me that given that you already have the cathode, try to retrofit
At 12:42 AM 6/17/2008, you wrote:
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==============================Original Headers============================== 6, 20 -- From gary-at-gaugler.com Fri Jun 20 00:41:32 2008 6, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5K5fVu0024174 6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jun 2008 00:41:32 -0500 6, 20 -- Message-Id: {200806200541.m5K5fVu0024174-at-ns.microscopy.com} 6, 20 -- Received: (qmail 10575 invoked from network); 19 Jun 2008 22:40:59 -0700 6, 20 -- Received: by simscan 1.1.0 ppid: 10572, pid: 10573, t: 0.1167s 6, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 6, 20 -- by smtp2 with SMTP; 19 Jun 2008 22:40:59 -0700 6, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 20 -- Date: Thu, 19 Jun 2008 19:43:22 -0700 6, 20 -- To: bengu-at-fen.bilkent.edu.tr 6, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 20 -- Subject: Re: [Microscopy] SEM- LaB6 filament change out 6, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 6, 20 -- In-Reply-To: {200806170742.m5H7gEEc006154-at-ns.microscopy.com} 6, 20 -- References: {200806170742.m5H7gEEc006154-at-ns.microscopy.com} 6, 20 -- Mime-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-77E2D0C ==============================End of - Headers==============================
Hi Necat! I second all the advices of Vlad (hope you saw them, Vlad is always very helpful): carbonate-free NaOH, rincing in diluted NaOH after lead staining... I don't really see how your problem could be dirt if you see it on thin sections and not on thick sections. What you could do is to cut at the same thickness with glass knife and diamond knife. For example both at 200 nm. Then you will at least know if the problem is related to the thickness or to the knife. Best regards,
Stephane
----- Original Message ---- X-from: "nyilmaz-at-mersin.edu.tr" {nyilmaz-at-mersin.edu.tr} To: nizets2-at-yahoo.com Sent: Wednesday, June 18, 2008 10:41:09 AM
Hello again,
I'd like to thank you first, for your kindly efforts to help solving my precipitation problem. I'm very pleased to be a member of the group. I put some sample pictures with precipitation problem for examining on a web page its link given below. I wrote also our detailed solution preparation, processing and contrasting protocols below. Additionally, we checked our sections without contrasting and contrasting with UA only and we didn't find any problem.
Thanks in advance...
Necat Yilmaz
Link: http://electron-microscopy.blogspot.com/
PROTOCOLS:
Processing:
1- Fixation in %2.5 cold GA 4-6 hours 2- Washing in cold Phosphate Buffer 3- Post-fixation in %1 cold osmic acid 1 hour 4- Washing in cold Phosphate Buffer 5- Dehydration in graded cold alcohols 6- Propilen oxide 2X15 min 7- Propilen+resin mixture 3x30 min 8- Resin with agitation overnight 9- Embedding
Staining:
1- Floating (I don't know why?) on UA drop 5-10 min 2- Washing in carbonate free dH2O 3- Floating on lead citrate 2 min 4- Washing in carbonate free dH2O
Preparation of solutions:
UA: 1- Saturation in dH2O 2- Filtering with Whatman filter paper 3- Filtering with 0.22
Lead Citrate: 1- 1.33 gr Lead nitrate 2- 1.76 sodium citrate 3- Mix in 30 ml carbonate free dH2O for 30 min 4- Add 8 ml 1 N NaOH 5- Add 12 ml carbonate free dH2O
==============================Original Headers============================== 14, 32 -- From nyilmaz-at-mersin.edu.tr Wed Jun 18 03:35:58 2008 14, 32 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5I8Zmlk020793 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 03:35:56 -0500 14, 32 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 14, 32 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 9461E2244A4 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 11:34:42 +0300 (EEST) 14, 32 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 14, 32 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 14, 32 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 14, 32 -- with ESMTP id FNoBSKViy5NK for {Microscopy-at-microscopy.com} ; 14, 32 -- Wed, 18 Jun 2008 11:34:12 +0300 (EEST) 14, 32 -- Received: from nejat (unknown [193.255.129.131]) 14, 32 -- by mail.mersin.edu.tr (Postfix) with SMTP id 0B99B2244F4 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 18 Jun 2008 11:34:03 +0300 (EEST) 14, 32 -- Message-ID: {002701c8d11e$587d7460$6b01a8c0-at-nejat} 14, 32 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 14, 32 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 14, 32 -- Subject: does staining artifact occur due to section thickness-2 14, 32 -- Date: Wed, 18 Jun 2008 11:35:50 +0300 14, 32 -- MIME-Version: 1.0 14, 32 -- Content-Type: text/plain; 14, 32 -- format=flowed; 14, 32 -- charset="iso-8859-9"; 14, 32 -- reply-type=original 14, 32 -- Content-Transfer-Encoding: 7bit 14, 32 -- X-Priority: 3 14, 32 -- X-MSMail-Priority: Normal 14, 32 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 14, 32 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 14, 32 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 14, 32 -- X-Virus-Scanned: ClamAV using ClamSMTP ==============================End of - Headers==============================
==============================Original Headers============================== 27, 21 -- From nizets2-at-yahoo.com Fri Jun 20 01:48:12 2008 27, 21 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 27, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5K6mCZA006311 27, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jun 2008 01:48:12 -0500 27, 21 -- Received: (qmail 84177 invoked by uid 60001); 20 Jun 2008 06:47:35 -0000 27, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 27, 21 -- s=s1024; d=yahoo.com; 27, 21 -- h=Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 27, 21 -- b=qGU0Dub5fIRUXEB1yATQR7vbzQpUZqnDlGgawJopPbu9ADRBzcz4LAHoaS2w2ty+Cw6yFDIqLtYwSsERmiAee4q5vFlKjeeXpEjbVU1FL0G1JqfS/x8vgykFyfzvhH0MzMx/ORWAJrhyWQ/XNBDJWWXskActbL2JtA9tgF7TSPQ=; 27, 21 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Thu, 19 Jun 2008 23:47:35 PDT 27, 21 -- X-Mailer: YahooMailRC/975.45 YahooMailWebService/0.7.199 27, 21 -- Date: Thu, 19 Jun 2008 23:47:35 -0700 (PDT) 27, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 27, 21 -- Subject: Re: [Microscopy] does staining artifact occur due to section thickness-2 27, 21 -- To: nyilmaz-at-mersin.edu.tr 27, 21 -- Cc: microscopy-at-microscopy.com 27, 21 -- MIME-Version: 1.0 27, 21 -- Content-Type: text/plain; charset=iso-8859-1 27, 21 -- Message-ID: {500385.84066.qm-at-web37402.mail.mud.yahoo.com} 27, 21 -- Content-Transfer-Encoding: 8bit 27, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5K6mCZA006311 ==============================End of - Headers==============================
If you have an Ultracut S that's been mothballed, been in storage, used for parts, etc. and it has a Leica Stereozoom 6 on it, can I have the Stereozoom? If the optics in the head are clear, not frosted like mine, and you're never going to use it, can I swap with you? My frosted one for your unfrosted one. I'll pay for shipping.
My old lady eyes are bad enough without having to look through that darn frosted binoc.
I like frosted flakes not frosted lenses.
Thanks and have a great weekend!
Paula :-)
Paula Sicurello VA Hospital San Diego Microscope Facility room B141 3350 La Jolla Village Drive San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 10, 24 -- From vapatpxs-at-yahoo.com Fri Jun 20 12:33:57 2008 10, 24 -- Received: from n69.bullet.mail.sp1.yahoo.com (n69.bullet.mail.sp1.yahoo.com [98.136.44.41]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5KHXvl6011040 10, 24 -- for {microscopy-at-microscopy.com} ; Fri, 20 Jun 2008 12:33:57 -0500 10, 24 -- Received: from [216.252.122.219] by n69.bullet.mail.sp1.yahoo.com with NNFMP; 20 Jun 2008 17:33:02 -0000 10, 24 -- Received: from [69.147.84.116] by t4.bullet.sp1.yahoo.com with NNFMP; 20 Jun 2008 17:33:02 -0000 10, 24 -- Received: from [127.0.0.1] by omp208.mail.sp1.yahoo.com with NNFMP; 20 Jun 2008 17:33:02 -0000 10, 24 -- X-Yahoo-Newman-Property: ymail-3 10, 24 -- X-Yahoo-Newman-Id: 293323.73848.bm-at-omp208.mail.sp1.yahoo.com 10, 24 -- Received: (qmail 12688 invoked by uid 60001); 20 Jun 2008 17:33:02 -0000 10, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 24 -- s=s1024; d=yahoo.com; 10, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 24 -- b=F8GPk7mdg7+KGTzX19AZ/t46FU5y5zicKhADjYNMdkLnXQCaB7hLvPESSfWNXVTKaMjwaXxcerOdvQRI9/J7n84dTIff2qGAee8d29IrEXQgGpx7Ce5YQ0Egm5p6qr3VHpVnVE6v3CL7+AFfliUUEnzX7vGwNTlpSGUNk9JL/hU=; 10, 24 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Fri, 20 Jun 2008 10:33:01 PDT 10, 24 -- X-Mailer: YahooMailWebService/0.7.199 10, 24 -- Date: Fri, 20 Jun 2008 10:33:01 -0700 (PDT) 10, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 24 -- Reply-To: vapatpxs-at-yahoo.com 10, 24 -- Subject: Leica StereoZoom 6 10, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=us-ascii 10, 24 -- Message-ID: {111883.12668.qm-at-web46101.mail.sp1.yahoo.com} ==============================End of - Headers==============================
I'm really appreciated for your replies about my problem. I'll try to perform all of your advices as soon as possible, and will inform you about results. Thank you again...
Necat Yilmaz
==============================Original Headers============================== 4, 31 -- From nyilmaz-at-mersin.edu.tr Fri Jun 20 12:44:13 2008 4, 31 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5KHiB5Q023059 4, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jun 2008 12:44:12 -0500 4, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id E6B82224AB2; 4, 31 -- Fri, 20 Jun 2008 20:41:11 +0300 (EEST) 4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 4, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 4, 31 -- with ESMTP id Hvnll7i8Iyvq; Fri, 20 Jun 2008 20:40:42 +0300 (EEST) 4, 31 -- Received: from nejat (unknown [88.224.52.50]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id F2E0C224AB3; 4, 31 -- Fri, 20 Jun 2008 20:40:41 +0300 (EEST) 4, 31 -- Message-ID: {002201c8d2fd$0fe565b0$0301a8c0-at-nejat} 4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 4, 31 -- Subject: Thanks for staining problem 4, 31 -- Date: Fri, 20 Jun 2008 20:37:01 +0300 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; 4, 31 -- format=flowed; 4, 31 -- charset="iso-8859-9"; 4, 31 -- reply-type=original 4, 31 -- Content-Transfer-Encoding: 7bit 4, 31 -- X-Priority: 3 4, 31 -- X-MSMail-Priority: Normal 4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 4, 31 -- X-Virus-Scanned: ClamAV using ClamSMTP ==============================End of - Headers==============================
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Email: gxx-at-u.washington.edu Name: Xiaoxia
Organization: The University of Washington
Title-Subject: [Filtered] Hitachi FB-2000A FIB
Question: Dear All:
Has anyone used Hitachi FB-2000A FIB? Is it good for TEM sample Prepration? Since the one I will use in made on 1998, I am not sure if this product is still on market. How about the service of Hitachi? Any opinon is good for me.
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Email: desilgl-at-mcmaster.ca Name: Glynis de Silveira
Organization: McMaster University
Title-Subject: [Filtered] Two POSTDOCTORAL FELLOW/RESEARCH ASSOCIATE Positions
Question: TWO POSITIONS: POSTDOCTORAL FELLOW/RESEARCH ASSOCIATE - ABERRATION CORRECTED TEM
INTRODUCTION TO THE CENTRE McMaster University is establishing the Canadian Centre for Electron Microscopy, a national facility funded by the Canada Foundation for Innovation, the Province of Ontario and McMaster University. The Centre currently operates a suite of scanning and transmission electron microscopes including a CM12 TEM, a JEOL 2010F (with GIF, EDS, Cryomicroscopy), a VG HB601 (with fully upgraded electronics and an Enfina EELS detector) and two aberration-corrected FEI-Titan 80-300. The centre also provides an extensive suite of sample preparation equipment including a Focused Ion Beam. The Centre has recently commissioned two aberrationñcorrected FEI Titan 80-300s One unit has two spherical aberration correctors, and monochromator while the second unit is fitted with a spherical aberration corrector (image) and a monochromator. The laboratory has been designed for outstanding environment specification. The centre is operated by the Brockhouse Institute for Materials Research and currently employs 5 technical staff, one facility manager and two research associates. The Centre is seeking outstanding candidates to fill two positions in electron microscopy at the postdoctoral fellowship (PDF) level and/or research associate (RA) level depending on qualification and experience. Application deadline is July 1st 2008 but submissions will be considered until the positions are filled.
Position Summary Postdoctoral Fellow (PDF)/Research Associate- Electron microscopy: The role of the PDF/RA within the Canadian Centre for Electron Microscopy (CCEM) is to conduct research with advanced electron microscopy in order to support users of the facility using TEM/STEM/EELS-based techniques. Salary is $Can 40,000-55,000 depending on level (PDF/RA and experience). The positions are initially for 2 years with possibility of extensions.
SUMMARY POSITION REQUIREMENTS:
1. A PhD or equivalent in materials science/engineering, chemistry or physics. 2. Advanced understanding and skills in the acquisition and interpretation of electron microscopy data in the physical sciences. 3. High quality research publications in international peer-reviewed journals in a relevant research field. 4. Demonstrated interest in research collaborations. 5. Expertise in at least one specialist area of TEM (CBED, HREM, EELS, HAADF, etc.).
For questions or submissions of your application (a CV, a list of publications, the names of 3 referees and letter of motivation), contact: Ms. Anne Reynolds (areynol-at-mcmaster.ca), Brockhouse Institute for Materials Research, McMaster University, 1280 Main Street W., Hamilton, Ontario, L8S 4M1, Canada areynol-at-mcmaster.ca
Gianluigi Botton Canada Research Chair in Microscopy of Nanoscale Materials Professor, Dept of Materials Science and Engineering Brockhouse Institute for Materials Research and Centre for Emerging Device Technologies McMaster University 1280 Main Street West Hamilton, Ontario, L8S 4M1
I have a deposit sample (calcite on metal) and I need to determine if the calcite has changed into a different form. I have seen aragonite crystals already with some of my rock fragment samples but I am not familiar with other crystal shapes of calcite. Does anybody has an SEM photo of calcite as dolomite or valerite? I would really appreciate if you will send me with one (mmiralles-at-pi.ac.ae).
Thank you so much.
Melina Miralles Lab Technician The Petroleum Institute United Arab Emirates
==============================Original Headers============================== 6, 29 -- From mmiralles-at-pi.ac.ae Mon Jun 23 04:45:52 2008 6, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5N9jpYL029059 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 04:45:52 -0500 6, 29 -- X-IronPort-AV: E=Sophos;i="4.27,689,1204488000"; 6, 29 -- d="scan'208";a="510458" 6, 29 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 6, 29 -- by mx1.pi.ac.ae with ESMTP; 23 Jun 2008 13:37:44 +0400 6, 29 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Mon, 23 Jun 2008 13:47:26 +0400 6, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="us-ascii" 6, 29 -- x-cr-hashedpuzzle: AbG/ CQZ9 Edyd EnyJ EzO1 Fhxz F8P6 H70P H78p IfMV I7e0 JCFG J8C/ KZ4z LJT0 LTTU;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{5B7E4518-C544-4F72-BC3B-93B6F865D7B3};bQBtAGkAcgBhAGwAbABlAHMAQABwAGkALgBhAGMALgBhAGUA;Mon, 23 Jun 2008 09:47:21 GMT;UwBFAE0AIABwAGgAbwB0AG8AcwAgAG8AZgAgAGQAbwBsAG8AbQBpAHQAZQAgAGEAbgBkACAAdgBhAGwAZQByAGkAdABlAA== 6, 29 -- x-cr-puzzleid: {5B7E4518-C544-4F72-BC3B-93B6F865D7B3} 6, 29 -- Content-class: urn:content-classes:message 6, 29 -- Subject: SEM photos of dolomite and valerite 6, 29 -- Date: Mon, 23 Jun 2008 13:47:21 +0400 6, 29 -- Message-ID: {D5603421C6303A46883F87E7968F285B04A307E0-at-pi-exm.PI.AC.AE} 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- Thread-Topic: SEM photos of dolomite and valerite 6, 29 -- Thread-Index: AcjVFiIkpUp9+9oSQBW+UWZ/vR8qpA== 6, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 6, 29 -- To: {microscopy-at-microscopy.com} 6, 29 -- X-OriginalArrivalTime: 23 Jun 2008 09:47:26.0054 (UTC) FILETIME=[24A04860:01C8D516] 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5N9jpYL029059 ==============================End of - Headers==============================
we have a polymerization problem with LR-White in beem-Capsules after polymerization in the heat. In many samples, not in all, and only around the tissue the resin becomes very brittle, the rest of the block is perfect polymerized. What is the reason for this and how is the problem solved?
Thanks in advance, Anne Heller
==============================Original Headers============================== 4, 30 -- From heller-at-uni-hohenheim.de Mon Jun 23 07:42:36 2008 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5NCgZjM015747 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 07:42:35 -0500 4, 30 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 762EB11D541 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 14:44:05 +0200 (CEST) 4, 30 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 4, 30 -- by localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 4, 30 -- with ESMTP id xk88xPsIXb3K for {Microscopy-at-microscopy.com} ; 4, 30 -- Mon, 23 Jun 2008 14:44:00 +0200 (CEST) 4, 30 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 320E211D544 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 14:43:59 +0200 (CEST) 4, 30 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 4, 30 -- id BB0466015B; Mon, 23 Jun 2008 14:43:13 +0200 (CEST) 4, 30 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 4, 30 -- by webmail.uni-hohenheim.de (IMP) with HTTP 4, 30 -- for {heller-at-10.0.0.5} ; Mon, 23 Jun 2008 14:43:13 +0200 4, 30 -- Message-ID: {1214224993.485f9a6143774-at-webmail.uni-hohenheim.de} 4, 30 -- Date: Mon, 23 Jun 2008 14:43:13 +0200 4, 30 -- From: heller-at-uni-hohenheim.de 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 -- Subject: LR-White polymerization problem in beem Capsules 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 4, 30 -- X-Originating-IP: 144.41.103.45 ==============================End of - Headers==============================
It could be a couple things, but we have had problems in the past with osmicated tissue causing polymerization problems. I think this is a problem especially with LR White that has been stored and is near or past its expiration date. In our case, the resin "curdled" and looked very much like clear cottage cheese. We were not able to save the sample.
Since the polymerization was fine except for right around the sample, it would appear that oxygen penetrating the BEEM capsule is not the problem. Rather it seems to indicate that something in the sample is causing it. If your sample is not treated with osmium, maybe dehydration was not sufficient, although my understanding is that LRW is fairly tolerant to residual water content.
Was there anything unusual in the specimen processing? More detail might be helpful.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: heller-at-uni-hohenheim.de [mailto:heller-at-uni-hohenheim.de] Sent: Monday, June 23, 2008 7:44 AM To: Tindall, Randy D.
Hi, all,
we have a polymerization problem with LR-White in beem-Capsules after polymerization in the heat. In many samples, not in all, and only around the tissue the resin becomes very brittle, the rest of the block is perfect polymerized. What is the reason for this and how is the problem solved?
Thanks in advance, Anne Heller
==============================Original Headers============================== 4, 30 -- From heller-at-uni-hohenheim.de Mon Jun 23 07:42:36 2008 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5NCgZjM015747 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 07:42:35 -0500 4, 30 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 762EB11D541 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 14:44:05 +0200 (CEST) 4, 30 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 4, 30 -- by localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 4, 30 -- with ESMTP id xk88xPsIXb3K for {Microscopy-at-microscopy.com} ; 4, 30 -- Mon, 23 Jun 2008 14:44:00 +0200 (CEST) 4, 30 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 320E211D544 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 14:43:59 +0200 (CEST) 4, 30 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 4, 30 -- id BB0466015B; Mon, 23 Jun 2008 14:43:13 +0200 (CEST) 4, 30 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 4, 30 -- by webmail.uni-hohenheim.de (IMP) with HTTP 4, 30 -- for {heller-at-10.0.0.5} ; Mon, 23 Jun 2008 14:43:13 +0200 4, 30 -- Message-ID: {1214224993.485f9a6143774-at-webmail.uni-hohenheim.de} 4, 30 -- Date: Mon, 23 Jun 2008 14:43:13 +0200 4, 30 -- From: heller-at-uni-hohenheim.de 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 --
Hi,
I would go along with all of Randy's suggestions: re Oso4, or even if the sample was particularly dark and dense polymerisation can be adversely affected. Old reagents: Definitely a possibility, with the catalyst and accelerator prime candidates. Also re the catalyst some suppliers have altered the stabilising agent to meet saftey regs for transportation. It may be worthwhile drying the weighed catalyst in a 37'C oven to drive this off before adding to the monomer.
It might also be worth your while trying the LR White accelerator method of polymerisation - but don't use the suggested proportions in the LR leaflet. With in-date accelerator (we replace after 6 months), use 1ul per ml, mix by gentle invertion then completely fill the capsule and fasten the lid. Surround the capsule in a crushed ice heat sink - and don't hang about as the resin will start to set in about 10mins and should be complete in 1 - 1.5hrs (you can check the bubble on top to see if polymerisation is complete). Embedding capsules: The harder polycarb capsules work best but as the BEEM capsules do not seem to be the problem with your oven cured blocks, they should be OK for the accelerator polymerisation method too.
Hope this helps, Alastair
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em
-----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: 23 June 2008 14:47 To: Mckinnon, Alastair D.
It could be a couple things, but we have had problems in the past with osmicated tissue causing polymerization problems. I think this is a problem especially with LR White that has been stored and is near or past its expiration date. In our case, the resin "curdled" and looked very much like clear cottage cheese. We were not able to save the sample.
Since the polymerization was fine except for right around the sample, it would appear that oxygen penetrating the BEEM capsule is not the problem. Rather it seems to indicate that something in the sample is causing it. If your sample is not treated with osmium, maybe dehydration was not sufficient, although my understanding is that LRW is fairly tolerant to residual water content.
Was there anything unusual in the specimen processing? More detail might be helpful.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: heller-at-uni-hohenheim.de [mailto:heller-at-uni-hohenheim.de] Sent: Monday, June 23, 2008 7:44 AM To: Tindall, Randy D.
Hi, all,
we have a polymerization problem with LR-White in beem-Capsules after polymerization in the heat. In many samples, not in all, and only around the tissue the resin becomes very brittle, the rest of the block is perfect polymerized. What is the reason for this and how is the problem solved?
Thanks in advance, Anne Heller
==============================Original Headers============================== 4, 30 -- From heller-at-uni-hohenheim.de Mon Jun 23 07:42:36 2008 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5NCgZjM015747 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 07:42:35 -0500 4, 30 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 762EB11D541 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 14:44:05 +0200 (CEST) 4, 30 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 4, 30 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 4, 30 -- by localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 4, 30 -- with ESMTP id xk88xPsIXb3K for {Microscopy-at-microscopy.com} ; 4, 30 -- Mon, 23 Jun 2008 14:44:00 +0200 (CEST) 4, 30 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 4, 30 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 320E211D544 4, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jun 2008 14:43:59 +0200 (CEST) 4, 30 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 4, 30 -- id BB0466015B; Mon, 23 Jun 2008 14:43:13 +0200 (CEST) 4, 30 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 4, 30 -- by webmail.uni-hohenheim.de (IMP) with HTTP 4, 30 -- for {heller-at-10.0.0.5} ; Mon, 23 Jun 2008 14:43:13 +0200 4, 30 -- Message-ID: {1214224993.485f9a6143774-at-webmail.uni-hohenheim.de} 4, 30 -- Date: Mon, 23 Jun 2008 14:43:13 +0200 4, 30 -- From: heller-at-uni-hohenheim.de 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 --
Hi,
I'm looking for a C-mount color camera for under $2000 US for routine transmitted light still images(brightfield/DIC/phase). I'll not be using it for fluorescence, so I don't need something very sensitive. I've found some under-$2k models that seemingly fit the bill from the following manufacturers and was wondering if people can comment on these or suggest others:
Pixera (Professional PIVCS10132) Lumenera (Infinity1-3) Pixelink (PL-A623-C and PL-B774-U)
Also, what are people's thoughts on CMOS vs. CCD? The one CMOS camera we've tried (not named above) seemed to be more noisy even under high-light conditions (low gain) than CCD and had trouble with white-balance/color fidelity.
Thanks, Esteban
-- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Core Facility University of Missouri 120 Bond Life Sciences Center Columbia, MO 65211
http://www.biotech.missouri.edu/mcc
(573)882-4895 (573)884-9676 fax
==============================Original Headers============================== 2, 23 -- From fernandezg-at-missouri.edu Thu Jun 12 16:02:15 2008 2, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5CL2Fwj019654 2, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jun 2008 16:02:15 -0500 2, 23 -- Received: from UM-XMAIL03.um.umsystem.edu ([209.106.228.3]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 2, 23 -- Thu, 12 Jun 2008 16:02:15 -0500 2, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 23 -- Content-class: urn:content-classes:message 2, 23 -- MIME-Version: 1.0 2, 23 -- Content-Type: text/plain; 2, 23 -- charset="iso-8859-1" 2, 23 -- Subject: LM: Opinions on color cameras for brightfield; CMOS vs. CCD 2, 23 -- Date: Thu, 12 Jun 2008 16:02:14 -0500 2, 23 -- Message-ID: {002C76CE90154E4EAD095153F371D71D043EFBFF-at-UM-XMAIL03.um.umsystem.edu} 2, 23 -- X-MS-Has-Attach: 2, 23 -- X-MS-TNEF-Correlator: 2, 23 -- Thread-Topic: Opinions on color cameras for brightfield; CMOS vs. CCD 2, 23 -- thread-index: AcjMyc8hV8BxLSvOTsy9aW6sVEgjQA== 2, 23 -- From: "Fernandez, Gerardo E." {fernandezg-at-missouri.edu} 2, 23 -- To: {microscopy-at-microscopy.com} 2, 23 -- X-OriginalArrivalTime: 12 Jun 2008 21:02:15.0012 (UTC) FILETIME=[9761BA40:01C8CCCF] 2, 23 -- Content-Transfer-Encoding: 8bit 2, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5CL2Fwj019654 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 21 -- From kjmorris-at-well.ox.ac.uk Tue Jun 24 04:10:59 2008 20, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 20, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5O9Awim009491 20, 21 -- for {Microscopy-at-Microscopy.Com} ; Tue, 24 Jun 2008 04:10:58 -0500 20, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 20, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 20, 21 -- id 1KB4Z4-0002Ub-H4 20, 21 -- for Microscopy-at-Microscopy.Com; Tue, 24 Jun 2008 10:11:50 +0100 20, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 20, 21 -- To: {Microscopy-at-Microscopy.Com} 20, 21 -- Subject: FW: [Microscopy] Re: LM: Opinions on color cameras for brightfield; CMOS vs. CCD 20, 21 -- Date: Tue, 24 Jun 2008 10:11:51 +0100 20, 21 -- Message-ID: {2F9F58B1995C49B8A561B7A154266217-at-CytoWhizz} 20, 21 -- MIME-Version: 1.0 20, 21 -- Content-Type: text/plain; 20, 21 -- charset="iso-8859-1" 20, 21 -- X-Mailer: Microsoft Office Outlook 11 20, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 20, 21 -- Thread-Index: AcjNc9bgLGbB9wKTQ2iJRVN/uygBvQDqoccgAS5g2SA= 20, 21 -- Content-Transfer-Encoding: 8bit 20, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5O9Awim009491 ==============================End of - Headers==============================
My institution is seeking to set up an oversight committee for the EM Facility here, an EMAC or Electron Microscopy Advisory Council. Its goals would be to oversee budget allocations and provide broad pedagogical overview for this interdisciplnary facility which, for the last 10 years, has had a director from the Life Sciences. We are seeking to put together an impartial governing body. But we are also a small institution, and probably don't need a large group. As is common in a powerful faculty-run setting, some here wish to select all the EMAC members from the faculty while I (who am in Administration) seek to include Admin folks, such as someone from the finanace office.
Any suggestions out there, or successful models for an EM oversight committee?
Partly we are doing this because we need to, to make sure that decisions in increasingly tight budget years fairly reflect the balanced needs of all the sciences here, and partly in order to meet granting agency general guidelines for infrastructure to show institutional support, but I can't find a written eg NSF guideline or even suggested ones. I just remember from attending several MSA sessions re granting agencies that such a committee is desirable. On the short term, we are gearing up to submit a grant proposal to replace one of our aging scopes such as the 25-year-old SEM and need to get our ducks lined up.
Can anyone share the makeup of their oversight committee, or help me find the (e.g.) NSF-recommended setup?
Thanks, all.
Ann
Ann H. Lehman Electron Microscopy Facility at Trinity College 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman
==============================Original Headers============================== 2, 25 -- From Ann.Lehman-at-trincoll.edu Wed Jun 25 04:58:53 2008 2, 25 -- Received: from wsmtp1.cc.trincoll.edu (wsmtp2.cc.trincoll.edu [157.252.10.109]) 2, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5P9wq37021700 2, 25 -- for {Microscopy-at-Microscopy.com} ; Wed, 25 Jun 2008 04:58:52 -0500 2, 25 -- Received: from hickory.cc.trincoll.edu ([157.252.15.139]) by wsmtp1.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); 2, 25 -- Wed, 25 Jun 2008 05:58:55 -0400 2, 25 -- Received: from exbe1.cc.trincoll.edu ([157.252.15.111]) by hickory.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); 2, 25 -- Wed, 25 Jun 2008 05:58:55 -0400 2, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 25 -- Content-class: urn:content-classes:message 2, 25 -- MIME-Version: 1.0 2, 25 -- Content-Type: text/plain; 2, 25 -- charset="iso-8859-1" 2, 25 -- Subject: Makeup of Facility Oversight Committee 2, 25 -- Date: Wed, 25 Jun 2008 05:58:54 -0400 2, 25 -- Message-ID: {8CF6A92CB628444FB3C757618CD280390565A8A7-at-exbe1.cmpcntr.tc.trincoll.edu} 2, 25 -- X-MS-Has-Attach: 2, 25 -- X-MS-TNEF-Correlator: 2, 25 -- Thread-Topic: Makeup of Facility Oversight Committee 2, 25 -- Thread-Index: AcjWpz2tn2VIzentQyqYwulwGKllSA== 2, 25 -- From: "Lehman, Ann R" {Ann.Lehman-at-trincoll.edu} 2, 25 -- To: {Microscopy-at-Microscopy.com} 2, 25 -- X-OriginalArrivalTime: 25 Jun 2008 09:58:55.0655 (UTC) FILETIME=[147C8B70:01C8D6AA] 2, 25 -- Content-Transfer-Encoding: 8bit 2, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5P9wq37021700 ==============================End of - Headers==============================
I have routinely found that administrators are not very helpful at the grass-roots level. However, they can be very helpful when a faculty advisory group comes in support of particular initiatives. I think part of that problem is a need to balance so many different responsibilities that they cannot and usually are reluctant to get down to the everyday supervision of core research facilities where they may not actually use and understand the technology involved. In my mind, this job is more suited to the faculty who utilize the facility in question.
One of the most important things in applying for equipment grants is the scientific justification for the equipment being requested as well as the management and oversight of the facility. The justification must come from the faculty, not the visions of the administration. It makes sense to me to have those same faculty members involved in the oversight as they can then see the needs and work harder to fill them.
I have also run into real road-blocks with the financial arm of this university. The business end does not understand the way science works and often imposes rules and regulations that actually are detrimental to running a core facility in a financially sound way. Interpretation of granting agency rules (usually vague)can vary significantly if you are looking at them from a pure business perspective rather than from an actual user perspective. I would rather have a faculty advisory group telling the financial people what needs to be done and following up to see that things are done in a constructive way (for the facility and to meet research objectives) than have a financial person sitting on an advisory panel dictating how it should work. Now, this may not happen if the right financial person is there but the chances are that it will in many cases.
Our oversight committee is drawn from all major schools within the university that utilize the facility. The committee members are to be appointed by the administration of that school, usually by the associate dean for research. The schools with highest financial input and use get two members while the smaller or less involved schools get 1 member. This keeps the committee manageable in size and proportioned based on overall use. Names are supplied to the administrators of those who use the facility on a regular basis and they are encouraged but not required to choose from this list. Committee members serve 3 year terms but may be reappointed. In addition, the PI's of successful major equipment proposals serve on the advisory committee for 3 years covering the purchase, installation and initial use of that equipment. The PI oversight is necessary based on major equipment proposal instructions. It is this committee that then reports back to the School/college administrators who then interact with the university administration as required.
We have always gotten very high marks on grant applications for the management plans and oversight committees so I think that speaks for the acceptance of this structure by both NSF and NIH.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: Ann Lehman {ann.lehman-at-trincoll.edu} } Reply-To: Ann Lehman {ann.lehman-at-trincoll.edu} } Date: Wed, 25 Jun 2008 05:01:46 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Makeup of Facility Oversight Committee } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Listers, } } My institution is seeking to set up an oversight committee for the EM Facility } here, an EMAC or Electron Microscopy Advisory Council. Its goals would be to } oversee budget allocations and provide broad pedagogical overview for this } interdisciplnary facility which, for the last 10 years, has had a director } from the Life Sciences. We are seeking to put together an impartial governing } body. But we are also a small institution, and probably don't need a large } group. As is common in a powerful faculty-run setting, some here wish to } select all the EMAC members from the faculty while I (who am in } Administration) seek to include Admin folks, such as someone from the finanace } office. } } Any suggestions out there, or successful models for an EM oversight committee? } } Partly we are doing this because we need to, to make sure that decisions in } increasingly tight budget years fairly reflect the balanced needs of all the } sciences here, and partly in order to meet granting agency general guidelines } for infrastructure to show institutional support, but I can't find a written } eg NSF guideline or even suggested ones. I just remember from attending } several MSA sessions re granting agencies that such a committee is desirable. } On the short term, we are gearing up to submit a grant proposal to replace one } of our aging scopes such as the 25-year-old SEM and need to get our ducks } lined up. } } Can anyone share the makeup of their oversight committee, or help me find the } (e.g.) NSF-recommended setup? } } Thanks, all. } } Ann } } Ann H. Lehman } Electron Microscopy Facility at Trinity College } 300 Summit Street } Hartford CT 06106 } v. 860-297-4289 } f. 860-297-2538 } e. ann.lehman-at-trincoll.edu } w. http://www.trincoll.edu/~alehman } } } ==============================Original Headers============================== } 2, 25 -- From Ann.Lehman-at-trincoll.edu Wed Jun 25 04:58:53 2008 } 2, 25 -- Received: from wsmtp1.cc.trincoll.edu (wsmtp2.cc.trincoll.edu } [157.252.10.109]) } 2, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m5P9wq37021700 } 2, 25 -- for {Microscopy-at-Microscopy.com} ; Wed, 25 Jun 2008 04:58:52 -0500 } 2, 25 -- Received: from hickory.cc.trincoll.edu ([157.252.15.139]) by } wsmtp1.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); } 2, 25 -- Wed, 25 Jun 2008 05:58:55 -0400 } 2, 25 -- Received: from exbe1.cc.trincoll.edu ([157.252.15.111]) by } hickory.cc.trincoll.edu with Microsoft SMTPSVC(6.0.3790.3959); } 2, 25 -- Wed, 25 Jun 2008 05:58:55 -0400 } 2, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 2, 25 -- Content-class: urn:content-classes:message } 2, 25 -- MIME-Version: 1.0 } 2, 25 -- Content-Type: text/plain; } 2, 25 -- charset="iso-8859-1" } 2, 25 -- Subject: Makeup of Facility Oversight Committee } 2, 25 -- Date: Wed, 25 Jun 2008 05:58:54 -0400 } 2, 25 -- Message-ID: } {8CF6A92CB628444FB3C757618CD280390565A8A7-at-exbe1.cmpcntr.tc.trincoll.edu} } 2, 25 -- X-MS-Has-Attach: } 2, 25 -- X-MS-TNEF-Correlator: } 2, 25 -- Thread-Topic: Makeup of Facility Oversight Committee } 2, 25 -- Thread-Index: AcjWpz2tn2VIzentQyqYwulwGKllSA== } 2, 25 -- From: "Lehman, Ann R" {Ann.Lehman-at-trincoll.edu} } 2, 25 -- To: {Microscopy-at-Microscopy.com} } 2, 25 -- X-OriginalArrivalTime: 25 Jun 2008 09:58:55.0655 (UTC) } FILETIME=[147C8B70:01C8D6AA] } 2, 25 -- Content-Transfer-Encoding: 8bit } 2, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m5P9wq37021700 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 31 -- From dsherman-at-purdue.edu Wed Jun 25 07:42:59 2008 10, 31 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PCgx8a008499 10, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 07:42:59 -0500 10, 31 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 10, 31 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m5PCgxYi007640 10, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 08:42:59 -0400 10, 31 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 10, 31 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m5PCgw0N023558 10, 31 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 08:42:59 -0400 10, 31 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 31 -- Wed, 25 Jun 2008 08:42:57 -0400 10, 31 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 10, 31 -- Wed, 25 Jun 2008 12:42:57 +0000 10, 31 -- User-Agent: Microsoft-Entourage/12.10.0.080409 10, 31 -- Date: Wed, 25 Jun 2008 08:42:55 -0400 10, 31 -- Subject: Re: [Microscopy] Makeup of Facility Oversight Committee 10, 31 -- From: Debby Sherman {dsherman-at-purdue.edu} 10, 31 -- To: Ann Lehman {ann.lehman-at-trincoll.edu} , 10, 31 -- "message to: MSA list" {microscopy-at-microscopy.com} 10, 31 -- Message-ID: {C487B58F.2EF62%dsherman-at-purdue.edu} 10, 31 -- Thread-Topic: [Microscopy] Makeup of Facility Oversight Committee 10, 31 -- Thread-Index: AcjWwP0xdAzXj4A3RKCum4kBbgXH7Q== 10, 31 -- In-Reply-To: {200806251001.m5PA1kgm025028-at-ns.microscopy.com} 10, 31 -- Mime-version: 1.0 10, 31 -- Content-type: text/plain; 10, 31 -- charset="US-ASCII" 10, 31 -- Content-transfer-encoding: 7bit 10, 31 -- X-OriginalArrivalTime: 25 Jun 2008 12:42:57.0138 (UTC) FILETIME=[FE77AD20:01C8D6C0] 10, 31 -- X-PMX-Version: 5.4.0.320885 10, 31 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
I'm having problems with a yeast prep for SEM. The cells are grown in liquid, fixed with glutaraldehyde followed by osmium. For mounting the yeasts, I have put them onto Millipore filters (0.22um, Type HA, native or carbon or sputtered both sides), adsorbed to carbon-coated glass treated with polylysine or polyethyleneimine (0.1%). I get nicely stuck cells, good distributions without pile-ups. I stuck them to polyethyleneimine treated aluminum foil (subsequently mounted via carbon tape) to make sure the substrate conductivity is not the issue and I get nice monolayers of cells. The cells have been critical-point dried or dried from HMDS. I sputter coat as I normally do (2-3 min at 5mA, 2.2kV in argon - should give ~12-16 nm Au:Pd), but the result in all cases is most cells charging badly (5kv, 55uA load current) in conditions that most biological samples I work with do not. I've experienced this with pollen grains and other rounded specimens and it looks like "textbook" images that JEOL uses of toner particles to illustrate the limited contact mounting issue - these are all samples that make little contact with the substrate; and in my case I can't press them into a carbon tape. (See Figs 24 and 25, http://www.jeol.com/sem/docs/sem_guide/guide.pdf)
I'm assuming that it is the limited contact area that is mostly shaded in the sputtering process that is causing my problem. Although sputtering does fairly well getting the sides of things, is this one of the cases where rotation while sputtering might be a help?
Any other ideas will be much appreciated!
Thanks,
Dale Callaham The University of Massachusetts -at- Amherst
==============================Original Headers============================== 8, 20 -- From dac-at-research.umass.edu Wed Jun 25 10:37:54 2008 8, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PFbnvt028302 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 10:37:53 -0500 8, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 8, 20 -- (authenticated bits=0) 8, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m5PFbmud031517 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 11:37:49 -0400 8, 20 -- Message-ID: {486274EC.7020702-at-research.umass.edu} 8, 20 -- Date: Wed, 25 Jun 2008 11:40:12 -0500 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 20 -- Reply-To: dac-at-research.umass.edu 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 20 -- Subject: SEM problem, round samples, charging 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Perpendicular coating can't cover the sides of 3D specimens very well or at all.
gary g.
At 08:40 AM 6/25/2008, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 20 -- From gary-at-gaugler.com Wed Jun 25 10:57:35 2008 7, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5PFvYv0009220 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 10:57:35 -0500 7, 20 -- Message-Id: {200806251557.m5PFvYv0009220-at-ns.microscopy.com} 7, 20 -- Received: (qmail 11462 invoked from network); 25 Jun 2008 08:57:33 -0700 7, 20 -- Received: by simscan 1.1.0 ppid: 11458, pid: 11459, t: 0.1196s 7, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 20 -- by smtp1 with SMTP; 25 Jun 2008 08:57:33 -0700 7, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 20 -- Date: Wed, 25 Jun 2008 08:57:33 -0700 7, 20 -- To: dac-at-research.umass.edu 7, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 20 -- Subject: Re: [Microscopy] SEM problem, round samples, charging 7, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200806251540.m5PFe3lj030814-at-ns.microscopy.com} 7, 20 -- References: {200806251540.m5PFe3lj030814-at-ns.microscopy.com} 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-5C7C64FA ==============================End of - Headers==============================
Seems like you've done most everything I would do for cells like that for SEM, and I don't recall having any serious charging problems as you describe. Yeah, could be a contact issue, and gyrational tilt-a-whirl coating in a vacuum evaporator might eliminate the charging.
But one option to consider, is the double osmium fix called "OTO". You apply the first osmium fix as usual (in dist. water), then rinse that out (dist. water) and add the "T", thiocarbohydrazide (TCH), usually dissolved by heating to saturated solution, then cooled and filtered before applying to the sample. Then rinse that out (dist. water) and apply a second osmium fix. Rinse that (dist. water) and proceed to dehydrate, etc. as usual.
The TCH acts as a mordant to bind in more osmium 2nd time, enough so that you get a lot of conductivity doped into the cells which results in no charging under the beam.
Years ago I had horrible charging problems looking at fungal colonies grown on agar. No matter how much gold I evaporated on, still charged. The OTO method totally eliminated the charging. I can send you protocol and reference off-line if you would like to try this.'
Gib -------- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
dac-at-research.umass.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm having problems with a yeast prep for SEM. The cells are grown in } liquid, fixed with glutaraldehyde followed by osmium. For mounting the } yeasts, I have put them onto Millipore filters (0.22um, Type HA, native } or carbon or sputtered both sides), adsorbed to carbon-coated glass } treated with polylysine or polyethyleneimine (0.1%). I get nicely stuck } cells, good distributions without pile-ups. I stuck them to } polyethyleneimine treated aluminum foil (subsequently mounted via carbon } tape) to make sure the substrate conductivity is not the issue and I get } nice monolayers of cells. The cells have been critical-point dried or } dried from HMDS. I sputter coat as I normally do (2-3 min at 5mA, 2.2kV } in argon - should give ~12-16 nm Au:Pd), but the result in all cases is } most cells charging badly (5kv, 55uA load current) in conditions that } most biological samples I work with do not. I've experienced this with } pollen grains and other rounded specimens and it looks like "textbook" } images that JEOL uses of toner particles to illustrate the limited } contact mounting issue - these are all samples that make little contact } with the substrate; and in my case I can't press them into a carbon } tape. (See Figs 24 and 25, } http://www.jeol.com/sem/docs/sem_guide/guide.pdf) } } } I'm assuming that it is the limited contact area that is mostly shaded } in the sputtering process that is causing my problem. Although } sputtering does fairly well getting the sides of things, is this one of } the cases where rotation while sputtering might be a help? } } Any other ideas will be much appreciated! } } } Thanks, } } Dale Callaham } The University of Massachusetts -at- Amherst --
==============================Original Headers============================== 8, 21 -- From ahlst007-at-umn.edu Wed Jun 25 11:13:59 2008 8, 21 -- Received: from mta-a2.tc.umn.edu (mta-a2.tc.umn.edu [134.84.119.206]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PGDxvF022593 8, 21 -- for {Microscopy-at-Microscopy.com} ; Wed, 25 Jun 2008 11:13:59 -0500 8, 21 -- Received: from x160-94-39-28.cbs.umn.edu (x160-94-39-28.cbs.umn.edu [160.94.39.28]) 8, 21 -- by mta-a2.tc.umn.edu (UMN smtpd) with ESMTP 8, 21 -- for {Microscopy-at-Microscopy.com} ; Wed, 25 Jun 2008 11:13:54 -0500 (CDT) 8, 21 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU 8, 21 -- Message-ID: {48626E35.7050600-at-umn.edu} 8, 21 -- Date: Wed, 25 Jun 2008 11:11:33 -0500 8, 21 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 8, 21 -- Reply-To: ahlst007-at-umn.edu 8, 21 -- Organization: Imaging Center UM 8, 21 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Microscopy-at-Microscopy.com 8, 21 -- Subject: Re: [Microscopy] SEM problem, round samples, charging 8, 21 -- References: {200806251542.m5PFg8mq000868-at-ns.microscopy.com} 8, 21 -- In-Reply-To: {200806251542.m5PFg8mq000868-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A commong problem that one of our SEM students, Amanda Best, had with pollen grains. I showed her how to get around the lack of coating on the bottom** using cardboard, which takes forever to degas, and she came up with a nice solution. Microscopy Today, July 2007, pg52 ("Microscopy 101"), SEM stub holders for sputter coating at 90 [degrees] tilt". The holders are modified shelf brackets, easily made. This is now a routine procedure for a couple of labs here. If you don't have the issue, it can be downloaded as a pdf from the MT website, http://www.microscopy-today.com
**This was addressed some years ago by Mary Fletcher at UBC, but I forget the print reference. Sorry Mary.
Phil
} Dear Listers, } } I'm having problems with a yeast prep for SEM. The cells are grown in } liquid, fixed with glutaraldehyde followed by osmium. For mounting the } yeasts, I have put them onto Millipore filters (0.22um, Type HA, native } or carbon or sputtered both sides), adsorbed to carbon-coated glass } treated with polylysine or polyethyleneimine (0.1%). I get nicely stuck } cells, good distributions without pile-ups. I stuck them to } polyethyleneimine treated aluminum foil (subsequently mounted via carbon } tape) to make sure the substrate conductivity is not the issue and I get } nice monolayers of cells. The cells have been critical-point dried or } dried from HMDS. I sputter coat as I normally do (2-3 min at 5mA, 2.2kV } in argon - should give ~12-16 nm Au:Pd), but the result in all cases is } most cells charging badly (5kv, 55uA load current) in conditions that } most biological samples I work with do not. I've experienced this with } pollen grains and other rounded specimens and it looks like "textbook" } images that JEOL uses of toner particles to illustrate the limited } contact mounting issue - these are all samples that make little contact } with the substrate; and in my case I can't press them into a carbon } tape. (See Figs 24 and 25, } http://www.jeol.com/sem/docs/sem_guide/guide.pdf) } } } I'm assuming that it is the limited contact area that is mostly shaded } in the sputtering process that is causing my problem. Although } sputtering does fairly well getting the sides of things, is this one of } the cases where rotation while sputtering might be a help? } } Any other ideas will be much appreciated! } } } Thanks, } } Dale Callaham } The University of Massachusetts -at- Amherst -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 26 -- From oshel1pe-at-cmich.edu Wed Jun 25 11:51:39 2008 5, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PGpcEW004655 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 11:51:39 -0500 5, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m5PGpZCt000849; 5, 26 -- Wed, 25 Jun 2008 12:51:38 -0400 5, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 5, 26 -- Wed, 25 Jun 2008 12:51:47 -0400 5, 26 -- Mime-Version: 1.0 5, 26 -- Message-Id: {f0624080bc48825cf7f43-at-[141.209.160.249]} 5, 26 -- In-Reply-To: {200806251542.m5PFgpQO001928-at-ns.microscopy.com} 5, 26 -- References: {200806251542.m5PFgpQO001928-at-ns.microscopy.com} 5, 26 -- Date: Wed, 25 Jun 2008 12:50:46 -0400 5, 26 -- To: dac-at-research.umass.edu 5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 26 -- Subject: Re: [Microscopy] SEM problem, round samples, charging 5, 26 -- Cc: Microscopy-at-microscopy.com 5, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 26 -- X-OriginalArrivalTime: 25 Jun 2008 16:51:47.0720 (UTC) FILETIME=[C1C9B080:01C8D6E3] 5, 26 -- X-Canit-CHI2: 0.00 5, 26 -- X-Bayes-Prob: 0.0001 (Score 0, tokens from: -at--at-RPTN) 5, 26 -- X-Spam-Score: -3.90 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE 5, 26 -- X-CanItPRO-Stream: default 5, 26 -- X-Canit-Stats-ID: 14811 - 7521f2326743 5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
These are the most common solutions when having problems with conductivity -
1. Incomplete coating - rectified by tilting the specimen + and - 45 by multiple coats if that is the only way.
2. Run at a low kV (that is what you are doing but could you go lower?)
3. Reduce the spot size beyond what is normal for the magnifications in use (less electrons hit the sample so less need to bleed away to earth)
4. Lower the emission current (less electrons hit the sample so less need to bleed away to earth)
5. Put on a slight positive tilt (increases the BSE contribution to the signal and BSE are less effected by charge)
6. If you have a dual SE detector system use the lower detector (increases the BSE contribution to the signal and BSE are less effected by charge)
Hope this helps
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {dac-at-research.umass.edu} To: {protrain-at-emcourses.com} Sent: Wednesday, June 25, 2008 4:39 PM
I watch Dennis Kunkel (http://www.denniskunkel.com) do this all the time, the cheap and easy way. He sputter coats from the top, then he lies the stubs on their sides in the coater and coats, then he rotates them a third of a turn and coats, then another third of a turn and coat, then another... and may finish off with another coating from the top if stuff is really piled up. It takes a little while, but doesn't require a tilt-and-rotate stage. We use the pin stubs, so the lie at about 50 degrees, I guess. If you have cylinders, they will lie at 90 degrees.
Aloha, Tina
} I'm having problems with a yeast prep for SEM. The cells are grown in } liquid, fixed with glutaraldehyde followed by osmium. For mounting the } yeasts, I have put them onto Millipore filters (0.22um, Type HA, native } or carbon or sputtered both sides), adsorbed to carbon-coated glass } treated with polylysine or polyethyleneimine (0.1%). I get nicely stuck } cells, good distributions without pile-ups. I stuck them to } polyethyleneimine treated aluminum foil (subsequently mounted via carbon } tape) to make sure the substrate conductivity is not the issue and I get } nice monolayers of cells. The cells have been critical-point dried or } dried from HMDS. I sputter coat as I normally do (2-3 min at 5mA, 2.2kV } in argon - should give ~12-16 nm Au:Pd), but the result in all cases is } most cells charging badly (5kv, 55uA load current) in conditions that } most biological samples I work with do not. I've experienced this with } pollen grains and other rounded specimens and it looks like "textbook" } images that JEOL uses of toner particles to illustrate the limited } contact mounting issue - these are all samples that make little contact } with the substrate; and in my case I can't press them into a carbon } tape. (See Figs 24 and 25, } http://www.jeol.com/sem/docs/sem_guide/guide.pdf) } } } I'm assuming that it is the limited contact area that is mostly shaded } in the sputtering process that is causing my problem. Although } sputtering does fairly well getting the sides of things, is this one of } the cases where rotation while sputtering might be a help? } } Any other ideas will be much appreciated! } } } Thanks, } } Dale Callaham } The University of Massachusetts -at- Amherst } } ==============================Original Headers============================== } 8, 20 -- From dac-at-research.umass.edu Wed Jun 25 10:37:54 2008 } 8, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PFbnvt028302 } 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 10:37:53 -0500 } 8, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) } 8, 20 -- (authenticated bits=0) } 8, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m5PFbmud031517 } 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 11:37:49 -0400 } 8, 20 -- Message-ID: {486274EC.7020702-at-research.umass.edu} } 8, 20 -- Date: Wed, 25 Jun 2008 11:40:12 -0500 } 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} } 8, 20 -- Reply-To: dac-at-research.umass.edu } 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 8, 20 -- Subject: SEM problem, round samples, charging } 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 20 -- Content-Transfer-Encoding: 7bit } 8, 20 -- X-Whitelist: TRUE } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 21 -- From tina-at-pbrc.hawaii.edu Wed Jun 25 13:02:11 2008 5, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PI29qW000356 5, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 13:02:10 -0500 5, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m5PI25nR024109 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 21 -- Wed, 25 Jun 2008 08:02:05 -1000 (HST) 5, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m5PI24rA024106; 5, 21 -- Wed, 25 Jun 2008 08:02:04 -1000 (HST) 5, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 21 -- Date: Wed, 25 Jun 2008 08:02:03 -1000 (HST) 5, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 21 -- X-Sender: tina-at-halia 5, 21 -- To: dac-at-research.umass.edu 5, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 21 -- Subject: Re: [Microscopy] SEM problem, round samples, charging 5, 21 -- In-Reply-To: {200806251539.m5PFdTUs029833-at-ns.microscopy.com} 5, 21 -- Message-ID: {Pine.GSO.4.21.0806250757490.24074-100000-at-halia} 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Thank you all. It sounds like "my" problem is well known and tilted coating by various means is a common and effective cure; and the price is right!
Dale
Tina Carvalho wrote: } I watch Dennis Kunkel (http://www.denniskunkel.com) do this all the time, } the cheap and easy way. He sputter coats from the top, then he lies the } stubs on their sides in the coater and coats, then he rotates them a third } of a turn and coats, then another third of a turn and coat, then } another... and may finish off with another coating from the top if stuff } is really piled up. It takes a little while, but doesn't require a } tilt-and-rotate stage. We use the pin stubs, so the lie at about 50 } degrees, I guess. If you have cylinders, they will lie at 90 degrees. } } Aloha, Tina } } } I'm having problems with a yeast prep for SEM. The cells are grown in } } liquid, fixed with glutaraldehyde followed by osmium. For mounting the } } yeasts, I have put them onto Millipore filters (0.22um, Type HA, native } } or carbon or sputtered both sides), adsorbed to carbon-coated glass } } treated with polylysine or polyethyleneimine (0.1%). I get nicely stuck } } cells, good distributions without pile-ups. I stuck them to } } polyethyleneimine treated aluminum foil (subsequently mounted via carbon } } tape) to make sure the substrate conductivity is not the issue and I get } } nice monolayers of cells. The cells have been critical-point dried or } } dried from HMDS. I sputter coat as I normally do (2-3 min at 5mA, 2.2kV } } in argon - should give ~12-16 nm Au:Pd), but the result in all cases is } } most cells charging badly (5kv, 55uA load current) in conditions that } } most biological samples I work with do not. I've experienced this with } } pollen grains and other rounded specimens and it looks like "textbook" } } images that JEOL uses of toner particles to illustrate the limited } } contact mounting issue - these are all samples that make little contact } } with the substrate; and in my case I can't press them into a carbon } } tape. (See Figs 24 and 25, } } http://www.jeol.com/sem/docs/sem_guide/guide.pdf) } } } } } } I'm assuming that it is the limited contact area that is mostly shaded } } in the sputtering process that is causing my problem. Although } } sputtering does fairly well getting the sides of things, is this one of } } the cases where rotation while sputtering might be a help? } } } } Any other ideas will be much appreciated! } } } } } } Thanks, } } } } Dale Callaham } } The University of Massachusetts -at- Amherst } } } } ==============================Original Headers============================== } } 8, 20 -- From dac-at-research.umass.edu Wed Jun 25 10:37:54 2008 } } 8, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) } } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PFbnvt028302 } } 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 10:37:53 -0500 } } 8, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) } } 8, 20 -- (authenticated bits=0) } } 8, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m5PFbmud031517 } } 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } } 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 11:37:49 -0400 } } 8, 20 -- Message-ID: {486274EC.7020702-at-research.umass.edu} } } 8, 20 -- Date: Wed, 25 Jun 2008 11:40:12 -0500 } } 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} } } 8, 20 -- Reply-To: dac-at-research.umass.edu } } 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 } } 8, 20 -- MIME-Version: 1.0 } } 8, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } 8, 20 -- Subject: SEM problem, round samples, charging } } 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 8, 20 -- Content-Transfer-Encoding: 7bit } } 8, 20 -- X-Whitelist: TRUE } } ==============================End of - Headers============================== } } } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** }
==============================Original Headers============================== 3, 22 -- From dac-at-research.umass.edu Wed Jun 25 13:15:22 2008 3, 22 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PIFMeL013568 3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 13:15:22 -0500 3, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 3, 22 -- (authenticated bits=0) 3, 22 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m5PIFKRi031122 3, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 3, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 14:15:21 -0400 3, 22 -- Message-ID: {486299D7.4050303-at-research.umass.edu} 3, 22 -- Date: Wed, 25 Jun 2008 14:17:43 -0500 3, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 3, 22 -- Reply-To: dac-at-research.umass.edu 3, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 3, 22 -- MIME-Version: 1.0 3, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 22 -- Subject: Re: [Microscopy] SEM problem, round samples, charging 3, 22 -- References: {Pine.GSO.4.21.0806250757490.24074-100000-at-halia} 3, 22 -- In-Reply-To: {Pine.GSO.4.21.0806250757490.24074-100000-at-halia} 3, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 3, 22 -- Content-Transfer-Encoding: 7bit 3, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
I spend considerable amount of my time analysing single grains of very rare minerals. Typically there is very little material available for probe analysis so I have to work with grains in the 100 - 200 um range. When I mount these grains in epoxy, I want to grind as little material as possible before I polish. At present, I smear a very thin layer of apiezon grease on a glass slide, get the grains on the grease and cover them with one drop of epoxy. When this first drop sets I drop a plastic mounting ring over the grains and fill as normal with epoxy. When the epoxy sets I can usually detach the glass from the ring and hopefully have the grain exposed on the surface of the mount ready for polishing.
In general this works, but I'm having some problems with the grease preventing the epoxy from sticking to the grain (This probably depends on the surface properties of the mineral). The surface of the epoxy is also wrinkled with this technique, making polishing difficult.
Does anyone have another techniquique that would work for this type of preparation? A substitute for the grease that would hold onto the mineral grains and allow removal of the glass slide would be really nice.
Thanks in advance
Glenn
Glenn Poirier
Earth Sciences Division
Canadain Museum of Nature
Ottawa, Ontario
(613) 364-4088
==============================Original Headers============================== 9, 28 -- From GPoirier-at-mus-nature.ca Wed Jun 25 13:22:13 2008 9, 28 -- Received: from mail165.messagelabs.com (mail165.messagelabs.com [216.82.253.147]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5PIMD7k026571 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 13:22:13 -0500 9, 28 -- X-VirusChecked: Checked 9, 28 -- X-Env-Sender: GPoirier-at-mus-nature.ca 9, 28 -- X-Msg-Ref: server-3.tower-165.messagelabs.com!1214418131!6450485!1 9, 28 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- 9, 28 -- X-Originating-IP: [207.61.24.131] 9, 28 -- Received: (qmail 8789 invoked from network); 25 Jun 2008 18:22:12 -0000 9, 28 -- Received: from mus-nature.ca (HELO NHBEXC01.mus-nature.ca) (207.61.24.131) 9, 28 -- by server-3.tower-165.messagelabs.com with SMTP; 25 Jun 2008 18:22:12 -0000 9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 28 -- Content-class: urn:content-classes:message 9, 28 -- MIME-Version: 1.0 9, 28 -- Content-Type: text/plain; 9, 28 -- charset="US-ASCII" 9, 28 -- Subject: Preparation of small, irreplaceable mineral grains for analysis 9, 28 -- Date: Wed, 25 Jun 2008 14:22:10 -0400 9, 28 -- Message-ID: {67B36D023E7A744BB4C9C83DCA80015002A19FFE-at-NHBEXC01.mus-nature.ca} 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- Thread-Topic: Preparation of small, irreplaceable mineral grains for analysis 9, 28 -- Thread-Index: AcjW8EPY8jAy/64zS+aQRxLUFnV7AgAAA2bg 9, 28 -- From: "Glenn Poirier" {GPoirier-at-mus-nature.ca} 9, 28 -- To: {microscopy-at-microscopy.com} 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5PIMD7k026571 ==============================End of - Headers==============================
When dealing with similar grains in our lab, we previously followed a procedure much like yours. Now, though, we drill holes in a disk made from a material harder than the set epoxy, usually acrylic or polycarbonate or occasionally even stainless steel. This provides some additional protection during polishing so that material doesn't wear away as quickly. The grains are held at the bottom of the holes using ordinary plastic ("invisible") tape. Our tape-epoxy combination doesn't seem to cause any setting problems -- no guarantees for anyone else's tape or epoxy. Any bubbles in the epoxy are removed by pumping down the mounts in a vacuum impregnator and, if necessary, a syringe with a hypodermic needle. This works pretty well for us -- your mileage may vary.
Best, Ellery
-------------------- Ellery E. Frahm Research Scientist/Manager Electron Microprobe Laboratory University of Minnesota - Twin Cities Department of Geology & Geophysics Lab Website: http://probelab.geo.umn.edu
On Jun 25, 2008, at 12:25 PM, GPoirier-at-mus-nature.ca wrote:
} Hello listers, } } I spend considerable amount of my time analysing single grains of very } rare minerals. Typically there is very little material available for } probe analysis so I have to work with grains in the 100 - 200 um } range. } When I mount these grains in epoxy, I want to grind as little material } as possible before I polish. At present, I smear a very thin layer of } apiezon grease on a glass slide, get the grains on the grease and } cover } them with one drop of epoxy. When this first drop sets I drop a } plastic } mounting ring over the grains and fill as normal with epoxy. When the } epoxy sets I can usually detach the glass from the ring and hopefully } have the grain exposed on the surface of the mount ready for } polishing. } } In general this works, but I'm having some problems with the grease } preventing the epoxy from sticking to the grain (This probably depends } on the surface properties of the mineral). The surface of the epoxy is } also wrinkled with this technique, making polishing difficult. } } Does anyone have another techniquique that would work for this type } of preparation? A substitute for the grease that would hold onto the } mineral grains and allow removal of the glass slide would be really } nice. } } Thanks in advance } } Glenn } } } } Glenn Poirier } } Earth Sciences Division } } Canadain Museum of Nature } } Ottawa, Ontario } } (613) 364-4088
==============================Original Headers============================== 8, 20 -- From frah0010-at-umn.edu Wed Jun 25 13:50:47 2008 8, 20 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PIolZT007964 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 13:50:47 -0500 8, 20 -- Received: from [172.19.144.67] ([198.202.202.20]) 8, 20 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 8, 20 -- Wed, 25 Jun 2008 13:50:43 -0500 (CDT) 8, 20 -- X-Umn-Remote-Mta: [N] - [198.202.202.20] #+TO+TS+AU+HN 8, 20 -- Cc: GPoirier-at-mus-nature.ca 8, 20 -- Message-Id: {9F0005E3-76F8-4748-B554-67746767D3B8-at-umn.edu} 8, 20 -- From: Ellery Frahm {frah0010-at-umn.edu} 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- In-Reply-To: {200806251825.m5PIPfwv002142-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- Mime-Version: 1.0 (Apple Message framework v924) 8, 20 -- Subject: Re: [Microscopy] Preparation of small, irreplaceable mineral grains for analysis 8, 20 -- Date: Wed, 25 Jun 2008 12:50:18 -0600 8, 20 -- References: {200806251825.m5PIPfwv002142-at-ns.microscopy.com} 8, 20 -- X-Mailer: Apple Mail (2.924) ==============================End of - Headers==============================
Try a dab of Loctite 4305 (UV curing) instead of grease. It is very low viscosity and will fully wet the sample. Also, it will fully adhere to your vacuum-impregnated epoxy, such as Struers Specifix-20, Buehlers Epo-Thin, etc....
Another way is to dab a bit of Gatan's G-1 and cure at 100C. Or, use an equivalent brand.
JQuinn
} From mail-at-ns.microscopy.com Wed Jun 25 14:20:46 2008 } Date: Wed, 25 Jun 2008 13:22:43 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: GPoirier-at-mus-nature.ca } Reply-to: GPoirier-at-mus-nature.ca } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Preparation of small, irreplaceable mineral grains for analysis } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hello listers, } } I spend considerable amount of my time analysing single grains of very } rare minerals. Typically there is very little material available for } probe analysis so I have to work with grains in the 100 - 200 um range. } When I mount these grains in epoxy, I want to grind as little material } as possible before I polish. At present, I smear a very thin layer of } apiezon grease on a glass slide, get the grains on the grease and cover } them with one drop of epoxy. When this first drop sets I drop a plastic } mounting ring over the grains and fill as normal with epoxy. When the } epoxy sets I can usually detach the glass from the ring and hopefully } have the grain exposed on the surface of the mount ready for polishing. } } In general this works, but I'm having some problems with the grease } preventing the epoxy from sticking to the grain (This probably depends } on the surface properties of the mineral). The surface of the epoxy is } also wrinkled with this technique, making polishing difficult. } } Does anyone have another techniquique that would work for this type } of preparation? A substitute for the grease that would hold onto the } mineral grains and allow removal of the glass slide would be really } nice. } } Thanks in advance } } Glenn } } } } Glenn Poirier } } Earth Sciences Division } } Canadain Museum of Nature } } Ottawa, Ontario } } (613) 364-4088 }
==============================Original Headers============================== 6, 12 -- From jquinn-at-www.matscieng.sunysb.edu Wed Jun 25 14:31:19 2008 6, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 6, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PJVJCs022160 6, 12 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 14:31:19 -0500 6, 12 -- Received: (from jquinn-at-localhost) 6, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m5PJTGd04369 6, 12 -- for microscopy-at-microscopy.com; Wed, 25 Jun 2008 15:29:16 -0400 6, 12 -- Date: Wed, 25 Jun 2008 15:29:16 -0400 6, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 6, 12 -- Message-Id: {200806251929.m5PJTGd04369-at-www.matscieng.sunysb.edu} 6, 12 -- To: microscopy-at-microscopy.com 6, 12 -- Subject: re: Preparation of small....... ==============================End of - Headers==============================
Hello all, We currently have available a 2007 Zeiss Axio Imager Z1 fluorescence microscope in beautiful condition. If anyone is interested please contact erin-at-aaisolutions.com and I will send you photos and specs. Thank you, Erin Curry (650) 324-2569 erin-at-aaisolutions.com
==============================Original Headers============================== 5, 30 -- From erin-at-aaisolutions.com Wed Jun 25 16:35:04 2008 5, 30 -- Received: from cuda1.brightberri.net (cuda1.brightberri.net [63.151.41.200]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PLZ4Pi006859 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Jun 2008 16:35:04 -0500 5, 30 -- X-ASG-Debug-ID: 1214429702-08a503bf0000-JFxeax 5, 30 -- X-Barracuda-URL: http://63.151.41.200:8000/cgi-bin/mark.cgi 5, 30 -- Received: from spock1.media3.net (localhost [127.0.0.1]) 5, 30 -- by cuda1.brightberri.net (Spam Firewall) with ESMTP id 7D5B3524C51 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 Jun 2008 17:35:02 -0400 (EDT) 5, 30 -- Received: from spock1.media3.net (spock1.media3.net [209.211.249.45]) by cuda1.brightberri.net with ESMTP id gwEwwE7LQNXxKOqQ for {Microscopy-at-Microscopy.Com} ; Wed, 25 Jun 2008 17:35:02 -0400 (EDT) 5, 30 -- Received: from Sales3 [64.81.17.181] by spock1.media3.net with ESMTP 5, 30 -- (SMTPD-10.0) id AA270344; Wed, 25 Jun 2008 17:35:35 -0400 5, 30 -- From: "Erin Curry" {erin-at-aaisolutions.com} 5, 30 -- To: {Microscopy-at-Microscopy.Com} 5, 30 -- X-ASG-Orig-Subj: Zeiss Axio Imager Z1 needs new home 5, 30 -- Subject: Zeiss Axio Imager Z1 needs new home 5, 30 -- Date: Wed, 25 Jun 2008 14:26:33 -0700 5, 30 -- Message-ID: {009f01c8d70a$24721440$6d563cc0$-at-com} 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; 5, 30 -- charset="US-ASCII" 5, 30 -- Content-Transfer-Encoding: 7bit 5, 30 -- X-Mailer: Microsoft Office Outlook 12.0 5, 30 -- thread-index: AcjXCiJQZDoeTQGOTXa3OuNyjCCMoQ== 5, 30 -- Content-Language: en-us 5, 30 -- x-cr-puzzleid: {97569E2A-654E-49AE-9CAD-02275B79E944} 5, 30 -- x-cr-hashedpuzzle: AITE Ak9T AtdW B2ve CHgo CTr4 Dh2/ Ec3j ErmS FfKi GKXK GYJp IbY3 I1PH L366 MC2T;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{97569E2A-654E-49AE-9CAD-02275B79E944};ZQByAGkAbgBAAGEAYQBpAHMAbwBsAHUAdABpAG8AbgBzAC4AYwBvAG0A;Wed, 25 Jun 2008 21:26:30 GMT;WgBlAGkAcwBzACAAQQB4AGkAbwAgAEkAbQBhAGcAZQByACAAWgAxACAAbgBlAGUAZABzACAAbgBlAHcAIABoAG8AbQBlAA== 5, 30 -- X-Barracuda-Connect: spock1.media3.net[209.211.249.45] 5, 30 -- X-Barracuda-Start-Time: 1214429702 5, 30 -- X-Barracuda-Virus-Scanned: by BriteBerri Spam Firewall at brightberri.net ==============================End of - Headers==============================
Hi Glenn, Something to try without the need for any grease: use a silicone rubber base rather than a glass slide. Epoxy doesn't stick to it and it has the advantage of being slightly tacky so your valuable small sample is harder to lose. In semiconductor manufacturing there are a variety of types and sizes sold under the name of 'Gel-Pak' which are used to transport things like diced up lasers - which can be as small as 100x200um. I use them as a work surface for TEM specimen prep since they are clean, solvent- and epoxy- resistant, and small fragments of material stay where you put them. You can also get silicone rubber inserts for petri dishes from microscopy houses such as Agar.
} -----Original Message----- } From: GPoirier-at-mus-nature.ca [mailto:GPoirier-at-mus-nature.ca] } Sent: 25 June 2008 19:26 } To: contact-at-integrityscientific.com } Subject: [Microscopy] Preparation of small, irreplaceable mineral grains } for analysis } } } } } ------------------------------------------------------------------------ -- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ -- } -- } } } } Hello listers, } } I spend considerable amount of my time analysing single grains of very } rare minerals. Typically there is very little material available for } probe analysis so I have to work with grains in the 100 - 200 um range. } When I mount these grains in epoxy, I want to grind as little material } as possible before I polish. At present, I smear a very thin layer of } apiezon grease on a glass slide, get the grains on the grease and cover } them with one drop of epoxy. When this first drop sets I drop a plastic } mounting ring over the grains and fill as normal with epoxy. When the } epoxy sets I can usually detach the glass from the ring and hopefully } have the grain exposed on the surface of the mount ready for polishing. } } In general this works, but I'm having some problems with the grease } preventing the epoxy from sticking to the grain (This probably depends } on the surface properties of the mineral). The surface of the epoxy is } also wrinkled with this technique, making polishing difficult. } } Does anyone have another techniquique that would work for this type } of preparation? A substitute for the grease that would hold onto the } mineral grains and allow removal of the glass slide would be really } nice. } } Thanks in advance } } Glenn } } } } Glenn Poirier } } Earth Sciences Division } } Canadain Museum of Nature } } Ottawa, Ontario } } (613) 364-4088 } } } ==============================Original } Headers============================== } 9, 28 -- From GPoirier-at-mus-nature.ca Wed Jun 25 13:22:13 2008 } 9, 28 -- Received: from mail165.messagelabs.com (mail165.messagelabs.com } [216.82.253.147]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } m5PIMD7k026571 } 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 13:22:13 - } 0500 } 9, 28 -- X-VirusChecked: Checked } 9, 28 -- X-Env-Sender: GPoirier-at-mus-nature.ca } 9, 28 -- X-Msg-Ref: server-3.tower- } 165.messagelabs.com!1214418131!6450485!1 } 9, 28 -- X-StarScan-Version: 5.5.12.14.2; banners=-,-,- } 9, 28 -- X-Originating-IP: [207.61.24.131] } 9, 28 -- Received: (qmail 8789 invoked from network); 25 Jun 2008 18:22:12 } -0000 } 9, 28 -- Received: from mus-nature.ca (HELO NHBEXC01.mus-nature.ca) } (207.61.24.131) } 9, 28 -- by server-3.tower-165.messagelabs.com with SMTP; 25 Jun 2008 } 18:22:12 -0000 } 9, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 28 -- Content-class: urn:content-classes:message } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- Content-Type: text/plain; } 9, 28 -- charset="US-ASCII" } 9, 28 -- Subject: Preparation of small, irreplaceable mineral grains for } analysis } 9, 28 -- Date: Wed, 25 Jun 2008 14:22:10 -0400 } 9, 28 -- Message-ID: } {67B36D023E7A744BB4C9C83DCA80015002A19FFE-at-NHBEXC01.mus-nature.ca} } 9, 28 -- X-MS-Has-Attach: } 9, 28 -- X-MS-TNEF-Correlator: } 9, 28 -- Thread-Topic: Preparation of small, irreplaceable mineral grains } for analysis } 9, 28 -- Thread-Index: AcjW8EPY8jAy/64zS+aQRxLUFnV7AgAAA2bg } 9, 28 -- From: "Glenn Poirier" {GPoirier-at-mus-nature.ca} } 9, 28 -- To: {microscopy-at-microscopy.com} } 9, 28 -- Content-Transfer-Encoding: 8bit } 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m5PIMD7k026571 } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 27 -- From contact-at-integrityscientific.com Wed Jun 25 16:53:07 2008 7, 27 -- Received: from mo-p07-ob.rzone.de (mo-p07-ob.rzone.de [81.169.146.190]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PLr4sH020207 7, 27 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 16:53:05 -0500 7, 27 -- X-RZG-CLASS-ID: mo07 7, 27 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSdpsjug98lWWvKGF2xNSAN7KN3iFqtxsalWerjYmhTm 7, 27 -- Received: from AnasPressie 7, 27 -- (host86-166-78-138.range86-166.btcentralplus.com [86.166.78.138]) 7, 27 -- by post.webmailer.de (mrclete mo9) (RZmta 16.45) 7, 27 -- with ESMTP id m02067k5PHtBJH ; Wed, 25 Jun 2008 23:53:01 +0200 (MEST) 7, 27 -- (envelope-from: {contact-at-integrityscientific.com} ) 7, 27 -- From: "Richard Beanland" {contact-at-integrityscientific.com} 7, 27 -- To: {GPoirier-at-mus-nature.ca} 7, 27 -- Cc: {microscopy-at-microscopy.com} 7, 27 -- Subject: RE: [Microscopy] Preparation of small, irreplaceable mineral grains for analysis 7, 27 -- Date: Wed, 25 Jun 2008 22:55:29 +0100 7, 27 -- Message-ID: {000d01c8d70e$2f135810$0201a8c0-at-AnasPressie} 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- Content-Transfer-Encoding: 7bit 7, 27 -- X-Priority: 3 (Normal) 7, 27 -- X-MSMail-Priority: Normal 7, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 7, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 7, 27 -- In-Reply-To: {200806251826.m5PIQSSv004091-at-ns.microscopy.com} 7, 27 -- Importance: Normal ==============================End of - Headers==============================
There have been several really good suggestions already. One technique I have used for embedding thick epoxy sections of biological material for resectioning for TEM examination involves using a release agent on the glass slide. One such product is available from EMS. You can find it in their online catalog at:
You coat a clean slide with this liquid, let it dry, then mount your sample onto it the same way you are now on your coated slide. Once the resin is cured, you can soak the prep in water and the release agent dissolves and you have a clean flat surface to polish. I don't know whether your particles would stay where you want them, though, since the surface is dry and hard.
-----Original Message----- X-from: GPoirier-at-mus-nature.ca [mailto:GPoirier-at-mus-nature.ca] Sent: Wednesday, June 25, 2008 12:26 PM To: jpchandl-at-mines.edu
Hello listers,
I spend considerable amount of my time analysing single grains of very rare minerals. Typically there is very little material available for probe analysis so I have to work with grains in the 100 - 200 um range. When I mount these grains in epoxy, I want to grind as little material as possible before I polish. At present, I smear a very thin layer of apiezon grease on a glass slide, get the grains on the grease and cover them with one drop of epoxy. When this first drop sets I drop a plastic mounting ring over the grains and fill as normal with epoxy. When the epoxy sets I can usually detach the glass from the ring and hopefully have the grain exposed on the surface of the mount ready for polishing.
In general this works, but I'm having some problems with the grease preventing the epoxy from sticking to the grain (This probably depends on the surface properties of the mineral). The surface of the epoxy is also wrinkled with this technique, making polishing difficult.
Does anyone have another techniquique that would work for this type of preparation? A substitute for the grease that would hold onto the mineral grains and allow removal of the glass slide would be really nice.
Thanks in advance
Glenn
Glenn Poirier
Earth Sciences Division
Canadain Museum of Nature
Ottawa, Ontario
(613) 364-4088
==============================Original Headers============================== 24, 21 -- From jpchandl-at-mines.edu Wed Jun 25 17:29:15 2008 24, 21 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) 24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PMTEpF001542 24, 21 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 17:29:14 -0500 24, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 24, 21 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id m5PMSvwB018113 24, 21 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 16:29:12 -0600 24, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} 24, 21 -- To: {microscopy-at-microscopy.com} 24, 21 -- References: {200806251825.m5PIPojA002499-at-ns.microscopy.com} 24, 21 -- Subject: RE: [Microscopy] Preparation of small, irreplaceable mineral grains for analysis 24, 21 -- Date: Wed, 25 Jun 2008 16:28:57 -0600 24, 21 -- Message-ID: {003201c8d712$dbf0be70$ad1c438a-at-mines.edu} 24, 21 -- MIME-Version: 1.0 24, 21 -- Content-Type: text/plain; 24, 21 -- charset="us-ascii" 24, 21 -- Content-Transfer-Encoding: 7bit 24, 21 -- X-Mailer: Microsoft Office Outlook 11 24, 21 -- Thread-Index: AcjW8Oc7GgGKICTBRJ6uNxszpJJ/FgAIIe7w 24, 21 -- In-Reply-To: {200806251825.m5PIPojA002499-at-ns.microscopy.com} 24, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I've used Rain-X, which is available at most hardware or automotive stores, for this very type of thing. You treat the slide with it and cured resin pops off very easily, no soaking required. It's cheap and a little bit goes a very long way.
Cheers, Jay
On Wed, Jun 25, 2008 at 5:30 PM, {jpchandl-at-mines.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Glenn, } } There have been several really good suggestions already. One technique I } have used for embedding thick epoxy sections of biological material for } resectioning for TEM examination involves using a release agent on the glass } slide. One such product is available from EMS. You can find it in their } online catalog at: } } http://www.emsdiasum.com/microscopy/products/preparation/coating.aspx#70880 } } You coat a clean slide with this liquid, let it dry, then mount your sample } onto it the same way you are now on your coated slide. Once the resin is } cured, you can soak the prep in water and the release agent dissolves and } you have a clean flat surface to polish. I don't know whether your } particles would stay where you want them, though, since the surface is dry } and hard. } } Good luck. } } --John } jpchandl-at-mines.edu } 303.384.2203 (office) } 970.219.5706 (cell) } } } -----Original Message----- } X-from: GPoirier-at-mus-nature.ca [mailto:GPoirier-at-mus-nature.ca] } Sent: Wednesday, June 25, 2008 12:26 PM } To: jpchandl-at-mines.edu } Subject: [Microscopy] Preparation of small, irreplaceable mineral grains for } analysis } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hello listers, } } I spend considerable amount of my time analysing single grains of very } rare minerals. Typically there is very little material available for } probe analysis so I have to work with grains in the 100 - 200 um range. } When I mount these grains in epoxy, I want to grind as little material } as possible before I polish. At present, I smear a very thin layer of } apiezon grease on a glass slide, get the grains on the grease and cover } them with one drop of epoxy. When this first drop sets I drop a plastic } mounting ring over the grains and fill as normal with epoxy. When the } epoxy sets I can usually detach the glass from the ring and hopefully } have the grain exposed on the surface of the mount ready for polishing. } } In general this works, but I'm having some problems with the grease } preventing the epoxy from sticking to the grain (This probably depends } on the surface properties of the mineral). The surface of the epoxy is } also wrinkled with this technique, making polishing difficult. } } Does anyone have another techniquique that would work for this type } of preparation? A substitute for the grease that would hold onto the } mineral grains and allow removal of the glass slide would be really } nice. } } Thanks in advance } } Glenn } } } } Glenn Poirier } } Earth Sciences Division } } Canadain Museum of Nature } } Ottawa, Ontario } } (613) 364-4088 } } } } } } ==============================Original Headers============================== } 24, 21 -- From jpchandl-at-mines.edu Wed Jun 25 17:29:15 2008 } 24, 21 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) } 24, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PMTEpF001542 } 24, 21 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 17:29:14 -0500 } 24, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) } 24, 21 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id m5PMSvwB018113 } 24, 21 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 16:29:12 -0600 } 24, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} } 24, 21 -- To: {microscopy-at-microscopy.com} } 24, 21 -- References: {200806251825.m5PIPojA002499-at-ns.microscopy.com} } 24, 21 -- Subject: RE: [Microscopy] Preparation of small, irreplaceable mineral grains for analysis } 24, 21 -- Date: Wed, 25 Jun 2008 16:28:57 -0600 } 24, 21 -- Message-ID: {003201c8d712$dbf0be70$ad1c438a-at-mines.edu} } 24, 21 -- MIME-Version: 1.0 } 24, 21 -- Content-Type: text/plain; } 24, 21 -- charset="us-ascii" } 24, 21 -- Content-Transfer-Encoding: 7bit } 24, 21 -- X-Mailer: Microsoft Office Outlook 11 } 24, 21 -- Thread-Index: AcjW8Oc7GgGKICTBRJ6uNxszpJJ/FgAIIe7w } 24, 21 -- In-Reply-To: {200806251825.m5PIPojA002499-at-ns.microscopy.com} } 24, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 37 -- From microtomy-at-gmail.com Wed Jun 25 18:02:27 2008 4, 37 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.156]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5PN2QII015157 4, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 18:02:27 -0500 4, 37 -- Received: by fg-out-1718.google.com with SMTP id 19so1435232fgg.4 4, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jun 2008 16:02:26 -0700 (PDT) 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=gmail.com; s=gamma; 4, 37 -- h=domainkey-signature:received:received:message-id:date:from:to 4, 37 -- :subject:in-reply-to:mime-version:content-type 4, 37 -- :content-transfer-encoding:content-disposition:references; 4, 37 -- bh=WW+c7TK0k79oICVwMxxPVANIn6sJF7AM/NfWTwycBiA=; 4, 37 -- b=TIyMTXhwKeD+skdIfPDKLpEF+f7AjVxeuF9+idDl7fQn5Y/lhn0g0AOZF9nn3+QbIB 4, 37 -- O0qzCJdeYv79pB9R8clCrGxrGmiib0EW1WqcKjgjmYfh2ZbPcoKFIA6be3DKFkb8nixN 4, 37 -- wCdt3TrnS8FwniCRiJBtCNrerNBewVPNLhAck= 4, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 37 -- d=gmail.com; s=gamma; 4, 37 -- h=message-id:date:from:to:subject:in-reply-to:mime-version 4, 37 -- :content-type:content-transfer-encoding:content-disposition 4, 37 -- :references; 4, 37 -- b=W8z3De388McUCqhTUXEihdrHd3Qi4Jqd63i9fXgs5v9N6uSuwwc5uKDeC9dAaIWYJM 4, 37 -- NIUStpWnVpAxKEt/zkFMYtw4pF7Tlq6zVxeaYh8V2rVirlsPHIQKJiV+bmwgtteiV+ax 4, 37 -- MrwBfeEC+sAnZnZBtwOYLc/v3OazCqxj+u9Do= 4, 37 -- Received: by 10.86.28.2 with SMTP id b2mr11220855fgb.78.1214434946497; 4, 37 -- Wed, 25 Jun 2008 16:02:26 -0700 (PDT) 4, 37 -- Received: by 10.86.77.2 with HTTP; Wed, 25 Jun 2008 16:02:21 -0700 (PDT) 4, 37 -- Message-ID: {ef6bda5c0806251602h7bd9a519j25e374a35cc9c094-at-mail.gmail.com} 4, 37 -- Date: Wed, 25 Jun 2008 18:02:21 -0500 4, 37 -- From: "Jay Campbell" {microtomy-at-gmail.com} 4, 37 -- To: microscopy {Microscopy-at-microscopy.com} 4, 37 -- Subject: Re: [Microscopy] RE: Preparation of small, irreplaceable mineral grains for analysis 4, 37 -- In-Reply-To: {200806252230.m5PMUYBS003623-at-ns.microscopy.com} 4, 37 -- MIME-Version: 1.0 4, 37 -- Content-Type: text/plain; charset=ISO-8859-1 4, 37 -- Content-Transfer-Encoding: 7bit 4, 37 -- Content-Disposition: inline 4, 37 -- References: {200806252230.m5PMUYBS003623-at-ns.microscopy.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both swtkeller-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TA-SICCO
Title-Subject: [Filtered] TEM-Looking to purchase used tripod type Polisher (complete)
Question: Hi: I am looking for a used (complete) tripod polisher in good to excellent condition that is not being used. Sandra
Hello, The scintillator material on our Zeiss Leo 430 SEM is partially rubbed off. I've seen places selling p-47 scintillator disks for replacement. Can these be epoxyed onto the end of the light pipe, because the current material is directly coating the end of the light pipe? Or, do I need to have the pipe recoated or purchase a whole new pipe/scintillator combination?
Thanks, Erik
Dr. Erik McCullen Senior Research Scientist Smart Sensors and Integrated Microsystems Wayne State University 3164 Engineering BLDG. Detroit, MI 48202
This is for the High Pressure Freezing Crowd who may be intro Extreme Cryo-Ultramicrotomy.
Has anyone ever tried mounting a sample-filled planchet (the little "top-hats" with the small, shallow wells) into a cryo-ultramicrotome and using a trimming tool to carve away the gold-coated metal and expose the sample? We don't have the special planchet-cutting accessory and don't want to unnecessarily risk our trimmer.
This is easily done with the copper tubes, but our freezer is bending the bejeezus out of our tubes for reasons as yet unknown, so we're stuck using planchets. We need to make cryosections directly from the HPF samples with no further processing and we can't use a filler/release agent like hexadecene. When we try to chip the sample (yeast) out of the planchets, the pieces come out too small to use.
Next time samples are ready, we might try using dialysis tubing, but it's too late for this run. Planchet trimming is our best current option. Does anyone have any hints or experience to share?
The reason we are limited in our techniques is that the end result will be precise elemental localization in the sample and we can't risk moving the elements around by exposing the yeast to potential contaminants or solvents.
Thanks for any thought!
Chills, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 10, 23 -- From TindallR-at-missouri.edu Thu Jun 26 15:15:32 2008 10, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QKFWmk009812 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 15:15:32 -0500 10, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Thu, 26 Jun 2008 15:15:27 -0500 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="us-ascii" 10, 23 -- Subject: Cryo EM/HPF: Microtomy problem 10, 23 -- Date: Thu, 26 Jun 2008 15:15:27 -0500 10, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD772E-at-UM-XMAIL08.um.umsystem.edu} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Cryo EM/HPF: Microtomy problem 10, 23 -- Thread-Index: AcjXyV+PUzzFhPEzQOeV3GkXfcrdrw== 10, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 26 Jun 2008 20:15:27.0515 (UTC) FILETIME=[5FC42EB0:01C8D7C9] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QKFWmk009812 ==============================End of - Headers==============================
Looking for some input for a small tray with side walls to catch small specimens dropped when observing under optical microscopes.
I can make something for this application and got a pretty good idea from a local jeweler but hope there may be someone who has bought something commercially.
This is in response to a student that lost a key thesis tooth fragment when it popped out of her tweezers while trying to place it under a microscope.
Any ideas welcomed.
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 23 -- From rbeavers-at-mail.smu.edu Thu Jun 26 15:29:10 2008 8, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QKTAZ2023980 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 15:29:10 -0500 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Thu, 26 Jun 2008 15:29:04 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="iso-8859-1" 8, 23 -- Subject: Tray for handling small specimens 8, 23 -- Date: Thu, 26 Jun 2008 15:29:03 -0500 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B304ABFCEB-at-s31xe7.systems.smu.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Tray for handling small specimens 8, 23 -- Thread-Index: AcjXy0YrgkLekGoGT4y/bhWSb6TPDQ== 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 26 Jun 2008 20:29:04.0243 (UTC) FILETIME=[4692FC30:01C8D7CB] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QKTAZ2023980 ==============================End of - Headers==============================
This is the question to ask Helmut Gnaegi of Diatome - to be safe. I do remember them showing a video a few years ago cutting a planchette with what I think was the regular Diatome trimming tool. Now, the planchette may have been a copper one...
Which freezer are you using, BTW?
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Begin forwarded message:
} From: TindallR-at-missouri.edu } Date: June 26, 2008 4:16:38 PM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] Cryo EM/HPF: Microtomy problem } Reply-To: TindallR-at-missouri.edu } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Collective, } } This is for the High Pressure Freezing Crowd who may be intro Extreme } Cryo-Ultramicrotomy. } } Has anyone ever tried mounting a sample-filled planchet (the little } "top-hats" with the small, shallow wells) into a cryo- } ultramicrotome and } using a trimming tool to carve away the gold-coated metal and } expose the } sample? We don't have the special planchet-cutting accessory and } don't } want to unnecessarily risk our trimmer. } } This is easily done with the copper tubes, but our freezer is bending } the bejeezus out of our tubes for reasons as yet unknown, so we're } stuck } using planchets. We need to make cryosections directly from the HPF } samples with no further processing and we can't use a filler/release } agent like hexadecene. When we try to chip the sample (yeast) out of } the planchets, the pieces come out too small to use. } } Next time samples are ready, we might try using dialysis tubing, but } it's too late for this run. Planchet trimming is our best current } option. Does anyone have any hints or experience to share? } } The reason we are limited in our techniques is that the end result } will } be precise elemental localization in the sample and we can't risk } moving } the elements around by exposing the yeast to potential contaminants or } solvents. } } Thanks for any thought! } } Chills, } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl? } Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } } } ==============================Original } Headers============================== } 10, 23 -- From TindallR-at-missouri.edu Thu Jun 26 15:15:32 2008 } 10, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um- } nsmtpout1.um.umsystem.edu [209.106.228.53]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m5QKFWmk009812 } 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 } 15:15:32 -0500 } 10, 23 -- Received: from UM-XMAIL08.um.umsystem.edu } ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 10, 23 -- Thu, 26 Jun 2008 15:15:27 -0500 } 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 10, 23 -- Content-class: urn:content-classes:message } 10, 23 -- MIME-Version: 1.0 } 10, 23 -- Content-Type: text/plain; } 10, 23 -- charset="us-ascii" } 10, 23 -- Subject: Cryo EM/HPF: Microtomy problem } 10, 23 -- Date: Thu, 26 Jun 2008 15:15:27 -0500 } 10, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD772E-at-UM- } XMAIL08.um.umsystem.edu} } 10, 23 -- X-MS-Has-Attach: } 10, 23 -- X-MS-TNEF-Correlator: } 10, 23 -- Thread-Topic: Cryo EM/HPF: Microtomy problem } 10, 23 -- Thread-Index: AcjXyV+PUzzFhPEzQOeV3GkXfcrdrw== } 10, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 10, 23 -- To: {microscopy-at-microscopy.com} } 10, 23 -- X-OriginalArrivalTime: 26 Jun 2008 20:15:27.0515 (UTC) } FILETIME=[5FC42EB0:01C8D7C9] } 10, 23 -- Content-Transfer-Encoding: 8bit } 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m5QKFWmk009812 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From vladislav_speransky-at-nih.gov Thu Jun 26 15:58:05 2008 8, 23 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QKw5Zr008656 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 15:58:05 -0500 8, 23 -- X-IronPortListener: NIH_Relay 8, 23 -- X-SBRS: None 8, 23 -- X-IronPort-AV: E=Sophos;i="4.27,710,1204520400"; 8, 23 -- d="scan'208";a="199547895" 8, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 8, 23 -- by nihrelayxway4.hub.nih.gov with ESMTP; 26 Jun 2008 16:58:05 -0400 8, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 8, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m5QKw4Tv002460 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 16:58:04 -0400 8, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 8, 23 -- References: {200806262016.m5QKGcxI011198-at-ns.microscopy.com} 8, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 23 -- Message-Id: {23E4069D-75F4-4EB4-9523-AA630E67A2C2-at-nih.gov} 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 23 -- Subject: Fwd: [Microscopy] Cryo EM/HPF: Microtomy problem 8, 23 -- Date: Thu, 26 Jun 2008 16:57:28 -0400 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
I've found tiny pieces can jump feet away from microscopes. Some I have yet to find! How far something goes depends on the pressure on the forceps and the angle of the hand holding them. I doubt that a small tray would catch most fragments. I would think a curtain that surrounds the sides and back of the scope would stop the object from going further then add a large ridged or slightly sticky rubber mat on the counter to catch the piece before it rolled further. That should catch about 75%.
How about prevention? Could you use a single chambered slide to capture the piece before putting it on the scope stage? That way it would not be lost if the slide jumped a bit as it was put on the scope stage.
Borrow an inverted microscope from the Life Sciences Department if the magnification requirement is equal. Working from the top is much easier to do.
Another thought...a small piece of double sticky tape on the slide with the object pushed up against the side of the tape. Not good if using high power since the objective would also stick when it got close to the piece of interest. Just about anything that avoids using the forceps at such an angle as is necessary to get something under an objective would be helpful.
All sorts of things are going through my brain like a thin coating of honey on the slide so the tooth could be put on easily, stick during observation, easily washed clean with water...
"Small tray with side walls" - a square Petri dish? What size are you thinking of?
Luck to you, Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov
} From: {rbeavers-at-mail.smu.edu} } Reply-To: {rbeavers-at-mail.smu.edu} } Date: Thu, 26 Jun 2008 15:32:28 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] Tray for handling small specimens
} Group, } } Looking for some input for a small tray with side walls to catch small } specimens dropped when observing under optical microscopes. } } I can make something for this application and got a pretty good idea from a } local jeweler but hope there may be someone who has bought something } commercially. } } This is in response to a student that lost a key thesis tooth fragment when it } popped out of her tweezers while trying to place it under a microscope. } } Any ideas welcomed. } } Roy Beavers } Southern Methodist University } Department of Earth Sciences } P.O. Box 750395 } Dallas, TX 75275 } Voice: 214-768-2756 } Fax: 214-768-2701 } Email: rbeavers-at-smu.edu
==============================Original Headers============================== 13, 27 -- From connellyps-at-nhlbi.nih.gov Thu Jun 26 16:15:34 2008 13, 27 -- Received: from nihxway6out.hub.nih.gov (nihxway6out.hub.nih.gov [128.231.90.114]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QLFYim024239 13, 27 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 16:15:34 -0500 13, 27 -- X-IronPortListener: Outbound_SMTP 13, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) 13, 27 -- by nihxway6out.hub.nih.gov with ESMTP; 26 Jun 2008 17:15:21 -0400 13, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 13, 27 -- Thu, 26 Jun 2008 17:15:17 -0400 13, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.169]) with Microsoft Exchange Server HTTP-DAV ; 13, 27 -- Thu, 26 Jun 2008 21:15:16 +0000 13, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 13, 27 -- Date: Thu, 26 Jun 2008 17:11:29 -0400 13, 27 -- Subject: Re: [Microscopy] Tray for handling small specimens 13, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 13, 27 -- To: {rbeavers-at-mail.smu.edu} , 13, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 13, 27 -- Message-ID: {C4897E41.1F6A%connellyps-at-nhlbi.nih.gov} 13, 27 -- Thread-Topic: [Microscopy] Tray for handling small specimens 13, 27 -- Thread-Index: AcjX0TNecaZUIEPEEd2j3gANk2Yv1A== 13, 27 -- In-Reply-To: {200806262032.m5QKWS6q032061-at-ns.microscopy.com} 13, 27 -- Mime-version: 1.0 13, 27 -- Content-type: text/plain; 13, 27 -- charset="ISO-8859-1" 13, 27 -- X-OriginalArrivalTime: 26 Jun 2008 21:15:17.0381 (UTC) FILETIME=[BB7E4350:01C8D7D1] 13, 27 -- Content-Transfer-Encoding: 8bit 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QLFYim024239 ==============================End of - Headers==============================
I thought you might've been putting tubes into planchettes on a Balzers. Can't Leica make their machine work?!.
Vlad
Begin forwarded message:
} From: "Tindall, Randy D." {TindallR-at-missouri.edu} } Date: June 26, 2008 5:03:26 PM EDT } To: {vladislav_speransky-at-nih.gov} } Subject: RE: [Microscopy] Fwd: Cryo EM/HPF: Microtomy problem } } Good idea about Helmut. Didn't think of that. } } We're using the Leica original EmPact. } } Thanks for the reply, Vlad. } } Randy } } -----Original Message----- } From: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] } Sent: Thursday, June 26, 2008 3:59 PM } To: Tindall, Randy D. } Subject: [Microscopy] Fwd: Cryo EM/HPF: Microtomy problem } } } } } ---------------------------------------------------------------------- } -- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -- } ---- } } Randy, } } This is the question to ask Helmut Gnaegi of Diatome - to be safe. } I do } remember them showing a video a few years ago cutting a planchette } with } what I think was the regular Diatome trimming tool. Now, the } planchette } may have been a copper one... } } Which freezer are you using, BTW? } } Vlad } ________________________________________________ } Vlad Speransky, Staff Scientist } Supramolecular Structure and Function Resource National Institute of } Biomedical Imaging and Bioengineering, NIH } 13 South Dr, Rm. 3N17 MSC 5766 } Bethesda, MD 20892 } 301 496-3989 } vladislav_speransky-at-nih.gov } } } Begin forwarded message: } } } From: TindallR-at-missouri.edu } } Date: June 26, 2008 4:16:38 PM EDT } } To: vladislav_speransky-at-nih.gov } } Subject: [Microscopy] Cryo EM/HPF: Microtomy problem } } Reply-To: TindallR-at-missouri.edu } } } } } } } } } } --------------------------------------------------------------------- } } - } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } } - } } ------ } } } } Dear Collective, } } } } This is for the High Pressure Freezing Crowd who may be intro Extreme } } Cryo-Ultramicrotomy. } } } } Has anyone ever tried mounting a sample-filled planchet (the little } } "top-hats" with the small, shallow wells) into a cryo- ultramicrotome } } and using a trimming tool to carve away the gold-coated metal and } } expose the sample? We don't have the special planchet-cutting } } accessory and don't want to unnecessarily risk our trimmer. } } } } This is easily done with the copper tubes, but our freezer is bending } } the bejeezus out of our tubes for reasons as yet unknown, so we're } } stuck using planchets. We need to make cryosections directly from } } the } } } HPF samples with no further processing and we can't use a } } filler/release agent like hexadecene. When we try to chip the sample } } (yeast) out of the planchets, the pieces come out too small to use. } } } } Next time samples are ready, we might try using dialysis tubing, but } } it's too late for this run. Planchet trimming is our best current } } option. Does anyone have any hints or experience to share? } } } } The reason we are limited in our techniques is that the end result } } will be precise elemental localization in the sample and we can't } } risk } } } moving the elements around by exposing the yeast to potential } } contaminants or solvents. } } } } Thanks for any thought! } } } } Chills, } } Randy } } } } Randy Tindall } } Senior EM Specialist } } Electron Microscopy Core Facility---We Do Small Well! } } W125 Veterinary Medicine } } University of Missouri } } Columbia, MO 65211 } } Tel: (573) 882-8304 } } Fax: (573) 884-2227 } } Email: tindallr-at-missouri.edu } } Web: http://www.emc.missouri.edu } } On-line calendar: } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl? } } Op=Splash&Amount= } } Week&NavType=Both&Type=TimePlan } } } } } } ==============================Original } } Headers============================== } } 10, 23 -- From TindallR-at-missouri.edu Thu Jun 26 15:15:32 2008 10, 23 } } -- Received: from um-nsmtpout1.um.umsystem.edu (um- } } nsmtpout1.um.umsystem.edu [209.106.228.53]) } } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m5QKFWmk009812 } } 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 } } 15:15:32 -0500 } } 10, 23 -- Received: from UM-XMAIL08.um.umsystem.edu } } ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft } } SMTPSVC(6.0.3790.3959); } } 10, 23 -- Thu, 26 Jun 2008 15:15:27 -0500 } } 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- } } Content-class: urn:content-classes:message 10, 23 -- MIME-Version: } } 1.0 } } } 10, 23 -- Content-Type: text/plain; } } 10, 23 -- charset="us-ascii" } } 10, 23 -- Subject: Cryo EM/HPF: Microtomy problem 10, 23 -- Date: } } Thu, } } } 26 Jun 2008 15:15:27 -0500 10, 23 -- Message-ID: } } {91108EF9255B394CBF8B7E3789814A4103CD772E-at-UM- } } XMAIL08.um.umsystem.edu} } } 10, 23 -- X-MS-Has-Attach: } } 10, 23 -- X-MS-TNEF-Correlator: } } 10, 23 -- Thread-Topic: Cryo EM/HPF: Microtomy problem 10, 23 -- } } Thread-Index: AcjXyV+PUzzFhPEzQOeV3GkXfcrdrw== 10, 23 -- From: } } "Tindall, Randy D." {TindallR-at-missouri.edu} 10, 23 -- To: } } {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 26 Jun } } 2008 20:15:27.0515 (UTC) FILETIME=[5FC42EB0:01C8D7C9] 10, 23 -- } } Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com id m5QKFWmk009812 } } ==============================End of - } } Headers============================== } } } ==============================Original } Headers============================== } 8, 23 -- From vladislav_speransky-at-nih.gov Thu Jun 26 15:58:05 2008 } 8, 23 } -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov } [128.231.90.98]) } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m5QKw5Zr008656 } 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 } 15:58:05 -0500 } 8, 23 -- X-IronPortListener: NIH_Relay } 8, 23 -- X-SBRS: None } 8, 23 -- X-IronPort-AV: E=Sophos;i="4.27,710,1204520400"; } 8, 23 -- d="scan'208";a="199547895" } 8, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) } 8, 23 -- by nihrelayxway4.hub.nih.gov with ESMTP; 26 Jun 2008 } 16:58:05 } -0400 } 8, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov } [128.231.217.77]) } 8, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id } m5QKw4Tv002460 } 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 } 16:58:04 -0400 } 8, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 8, 23 -- } References: {200806262016.m5QKGcxI011198-at-ns.microscopy.com} } 8, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed 8, 23 -- Message-Id: } {23E4069D-75F4-4EB4-9523-AA630E67A2C2-at-nih.gov} } 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- From: Vlad Speransky } {vladislav_speransky-at-nih.gov} 8, 23 -- Subject: Fwd: [Microscopy] Cryo } EM/HPF: Microtomy problem 8, 23 -- Date: Thu, 26 Jun 2008 16:57:28 } -0400 } 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- X-Mailer: Apple Mail } (2.753.1) ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 23 -- From vladislav_speransky-at-nih.gov Thu Jun 26 16:22:09 2008 5, 23 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QLM9SN002140 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 16:22:09 -0500 5, 23 -- X-IronPortListener: NIH_Relay 5, 23 -- X-SBRS: None 5, 23 -- X-IronPort-AV: E=Sophos;i="4.27,710,1204520400"; 5, 23 -- d="scan'208";a="24693081" 5, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 26 Jun 2008 17:22:08 -0400 5, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m5QLM86o003748 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 17:22:08 -0400 5, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 5, 23 -- References: {91108EF9255B394CBF8B7E3789814A4103CD7730-at-UM-XMAIL08.um.umsystem.edu} 5, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 23 -- Message-Id: {B29B6558-DC9E-42EE-B191-264C9F745928-at-nih.gov} 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 23 -- Subject: [Microscopy] Cryo EM/HPF: Microtomy problem 5, 23 -- Date: Thu, 26 Jun 2008 17:21:32 -0400 5, 23 -- To: Microscopy-at-microscopy.com 5, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Why not go to your local photography dealer and get an appropriate size of photo paper processing tray.These have many non-photo uses in our lab at Kodak. The largest ones (or 'liberated' cafeteria trays ) are great to hold rotary vacuum pumps that always choose the most inopportune moment to dump oil all over the lab :(...
==============================Original Headers============================== 2, 19 -- From jrminter-at-rochester.rr.com Thu Jun 26 17:03:02 2008 2, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QM32V7024651 2, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 17:03:02 -0500 2, 19 -- Received: from hrndva-web01-z02 ([10.128.132.152]) 2, 19 -- by hrndva-smta02.mail.rr.com with ESMTP 2, 19 -- id {20080626220301.THZG15198.hrndva-smta02.mail.rr.com-at-hrndva-web01-z02} 2, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 22:03:01 +0000 2, 19 -- Message-ID: {28877017.296121214517781993.JavaMail.root-at-hrndva-web01-z02} 2, 19 -- Date: Thu, 26 Jun 2008 18:03:01 -0400 2, 19 -- From: {jrminter-at-rochester.rr.com} 2, 19 -- To: Microscopy-at-microscopy.com 2, 19 -- Subject: Re: Tray for handling small specimens 2, 19 -- MIME-Version: 1.0 2, 19 -- Content-Type: text/plain; charset=utf-8 2, 19 -- Content-Transfer-Encoding: 7bit 2, 19 -- X-Priority: 3 (Normal) 2, 19 -- Sensitivity: Normal 2, 19 -- X-Originating-IP: from 67.240.238.57 by webmail.rochester.rr.com; Thu, 26 Jun 2008 22:03:01 +0000 ==============================End of - Headers==============================
Another alternative might be to get a small pump, which not only blows but sucks. You can get a variety of attachments to hold tiny things, or make them yourself. We use ours mainly as an air jet for cleaning things, but we can attach the hose to the other end and get suction. They come in a variety of sizes and are only one or two hundred dollars.
cheers, Rosemary
Dr Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 02-6246 5475 GPO Box 1600 fax. 02-6246 5334 Canberra, ACT 2601 Australia
On 27/6/08 7:22 AM, "connellyps-at-nhlbi.nih.gov" {connellyps-at-nhlbi.nih.gov} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Roy, } } I've found tiny pieces can jump feet away from microscopes. } Some I have yet to find! } How far something goes depends on the pressure on the forceps and the angle } of the hand holding them. I doubt that a small tray would catch most } fragments. } I would think a curtain that surrounds the sides and back of the scope would } stop the object from going further then add a large ridged or slightly } sticky rubber mat on the counter to catch the piece before it rolled } further. That should catch about 75%. } } How about prevention? Could you use a single chambered slide to capture the } piece before putting it on the scope stage? That way it would not be lost if } the slide jumped a bit as it was put on the scope stage. } } Borrow an inverted microscope from the Life Sciences Department if the } magnification requirement is equal. Working from the top is much easier to } do. } } Another thought...a small piece of double sticky tape on the slide with the } object pushed up against the side of the tape. Not good if using high power } since the objective would also stick when it got close to the piece of } interest. Just about anything that avoids using the forceps at such an angle } as is necessary to get something under an objective would be helpful. } } All sorts of things are going through my brain like a thin coating of honey } on the slide so the tooth could be put on easily, stick during observation, } easily washed clean with water... } } "Small tray with side walls" - a square Petri dish? What size are you } thinking of? } } Luck to you, } Pat } } Patricia Stranen Connelly } Biologist } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road South } Bldg. 14E Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-480-6560 } connellyps-at-mail.nih.gov } } } From: {rbeavers-at-mail.smu.edu} } } Reply-To: {rbeavers-at-mail.smu.edu} } } Date: Thu, 26 Jun 2008 15:32:28 -0500 } } To: {connellyps-at-nhlbi.nih.gov} } } Subject: [Microscopy] Tray for handling small specimens } } } Group, } } } } Looking for some input for a small tray with side walls to catch small } } specimens dropped when observing under optical microscopes. } } } } I can make something for this application and got a pretty good idea from a } } local jeweler but hope there may be someone who has bought something } } commercially. } } } } This is in response to a student that lost a key thesis tooth fragment when } } it } } popped out of her tweezers while trying to place it under a microscope. } } } } Any ideas welcomed. } } } } Roy Beavers } } Southern Methodist University } } Department of Earth Sciences } } P.O. Box 750395 } } Dallas, TX 75275 } } Voice: 214-768-2756 } } Fax: 214-768-2701 } } Email: rbeavers-at-smu.edu } } } } } ==============================Original Headers============================== } 13, 27 -- From connellyps-at-nhlbi.nih.gov Thu Jun 26 16:15:34 2008 } 13, 27 -- Received: from nihxway6out.hub.nih.gov (nihxway6out.hub.nih.gov } [128.231.90.114]) } 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m5QLFYim024239 } 13, 27 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 16:15:34 -0500 } 13, 27 -- X-IronPortListener: Outbound_SMTP } 13, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) } 13, 27 -- by nihxway6out.hub.nih.gov with ESMTP; 26 Jun 2008 17:15:21 -0400 } 13, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by } NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); } 13, 27 -- Thu, 26 Jun 2008 17:15:17 -0400 } 13, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by } NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server } mail.nih.gov ([156.40.71.169]) with Microsoft Exchange Server HTTP-DAV ; } 13, 27 -- Thu, 26 Jun 2008 21:15:16 +0000 } 13, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 13, 27 -- Date: Thu, 26 Jun 2008 17:11:29 -0400 } 13, 27 -- Subject: Re: [Microscopy] Tray for handling small specimens } 13, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} } 13, 27 -- To: {rbeavers-at-mail.smu.edu} , } 13, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 13, 27 -- Message-ID: {C4897E41.1F6A%connellyps-at-nhlbi.nih.gov} } 13, 27 -- Thread-Topic: [Microscopy] Tray for handling small specimens } 13, 27 -- Thread-Index: AcjX0TNecaZUIEPEEd2j3gANk2Yv1A== } 13, 27 -- In-Reply-To: {200806262032.m5QKWS6q032061-at-ns.microscopy.com} } 13, 27 -- Mime-version: 1.0 } 13, 27 -- Content-type: text/plain; } 13, 27 -- charset="ISO-8859-1" } 13, 27 -- X-OriginalArrivalTime: 26 Jun 2008 21:15:17.0381 (UTC) } FILETIME=[BB7E4350:01C8D7D1] } 13, 27 -- Content-Transfer-Encoding: 8bit } 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m5QLFYim024239 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 23 -- From prvs=Rosemary.White=056b6baf7-at-csiro.au Thu Jun 26 17:14:53 2008 10, 23 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QMEq1e006590 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 17:14:53 -0500 10, 23 -- X-IronPort-AV: E=Sophos;i="4.27,710,1204462800"; 10, 23 -- d="scan'208";a="2277809" 10, 23 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 10, 23 -- by act-ironport-int.csiro.au with ESMTP; 27 Jun 2008 08:14:51 +1000 10, 23 -- Received: from [152.83.167.45] ([152.83.167.45]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 10, 23 -- Fri, 27 Jun 2008 08:14:51 +1000 10, 23 -- User-Agent: Microsoft-Entourage/11.0.0.040405 10, 23 -- Date: Fri, 27 Jun 2008 08:20:11 +1000 10, 23 -- Subject: Re: [Microscopy] Re: Tray for handling small specimens 10, 23 -- From: Rosemary White {rosemary.white-at-csiro.au} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- Message-ID: {C48A533B.C6EC%rosemary.white-at-csiro.au} 10, 23 -- In-Reply-To: {200806262122.m5QLMGMo002344-at-ns.microscopy.com} 10, 23 -- Mime-version: 1.0 10, 23 -- Content-type: text/plain; 10, 23 -- charset="ISO-8859-1" 10, 23 -- X-OriginalArrivalTime: 26 Jun 2008 22:14:51.0228 (UTC) FILETIME=[0DAC09C0:01C8D7DA] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QMEq1e006590 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rao-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rao-at-lehigh.edu Name: Rao Karavadi
Organization: Lehigh University
Title-Subject: [Filtered] Re: [Microscopy] Tray for handling small specimens
Question: Dear Roy,
Handling TEM specimens with Tweezers is not a advisable. I do have similar kind of bad experiences in the past. The best way is to handle them vacuum pipette. You can buy 18" needles from SPI as well as tubing. Moreover, this is very economical too.
You can probably get small trays with side walls from PLANO Gmbh or Baltec AG. You can find their suppliers in US too, not very expensive though.
cheers Rao ----
Rao Karavadi Research Associate Lehigh University Bethlehem, PA 18015 Ph: 610-758-4002 Fax: 610-758-3526 Email:rao-at-lehigh.edu
I don't have the answer for the tray, but I would like to make some comments about handling small samples with tweezers.
I have taught the MicroCleave(TM) technique to a lot of students at a bunch of different universities over the years. These samples have at different stages of the process very thin, fragile samples and then ultimately very, very small samples that must be handled with the sharpest tweezers available under the stereomicroscope. There are two common bad habits that students have with respect to handling samples with tweezers. One is that they try to move the sample large distances. Often they do this without a catch basin such as their cupped hand. The other bad habit that they have is not to anchor their hand while they are manipulating the tweezers to position the sample. They will have their hand in the air trying to keep their hands steady. The following are points that I always seem to have to train students to do when handling very small and delicate samples.
1. If you have to move your samples in a tweezers a long distance, then use a self-closing or one that has a sliding O-ring to keep them closed while you move them and to cup one hand under the tweezers while doing so. Better yet, put the tweezers, with the sample held clamped in the tweezers, on a tray and then move the tray.
2. When positioning the sample, anchor the heel of your hand firmly and then just use small movements of your fingers to manipulate the tweezers.
3. If you are transferring the sample from one holder to another under a stereomicroscope, again, anchor your hand and don't move it. Lift the sample out of the holder and then move that holder out of place while holding the sample stationary and then move the second holder under the microscope under the sample. Then lower the sample into that holder. This way, the sample only makes a small distance move up and down while the hand is stabilized with the heel of your hand anchored.
4. If you can't anchor your hand on the desktop because you have to have the tweezers high off the desktop, then get a block, brick, or box that is rigid so that you can raise your hands to the correct height and still anchor the heel of your hand.
5. If you have delicate samples, then don't drink coffee or be hungry while you have to do the work, otherwise your hands will shake too much.
6. Never hold your samples where there is nothing to catch them other than the floor. If your hands aren't far from the table top, then they won't go far when you drop them. (Notice I said "when" and not "if".)
7. If you get a choice of colors for bench tops and counters, go with a single color, never go with a pattern, especially patterns that look like granite! Otherwise, you won't even find a screw that falls on the tabletop, let alone a sample.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Thursday, June 26, 2008 1:31 PM To: Walck-at-SouthBayTech.com
Group,
Looking for some input for a small tray with side walls to catch small specimens dropped when observing under optical microscopes.
I can make something for this application and got a pretty good idea from a local jeweler but hope there may be someone who has bought something commercially.
This is in response to a student that lost a key thesis tooth fragment when it popped out of her tweezers while trying to place it under a microscope.
Any ideas welcomed.
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 23 -- From rbeavers-at-mail.smu.edu Thu Jun 26 15:29:10 2008 8, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QKTAZ2023980 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 15:29:10 -0500 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Thu, 26 Jun 2008 15:29:04 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="iso-8859-1" 8, 23 -- Subject: Tray for handling small specimens 8, 23 -- Date: Thu, 26 Jun 2008 15:29:03 -0500 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B304ABFCEB-at-s31xe7.systems.smu.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Tray for handling small specimens 8, 23 -- Thread-Index: AcjXy0YrgkLekGoGT4y/bhWSb6TPDQ== 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 26 Jun 2008 20:29:04.0243 (UTC) FILETIME=[4692FC30:01C8D7CB] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QKTAZ2023980 ==============================End of - Headers==============================
No virus found in this incoming message. Checked by AVG. Version: 8.0.101 / Virus Database: 270.4.1/1521 - Release Date: 6/26/2008 11:20 AM
==============================Original Headers============================== 28, 23 -- From walck-at-southbaytech.com Thu Jun 26 19:34:18 2008 28, 23 -- Received: from nlpi087.prodigy.net (nlpi087.prodigy.net [207.115.36.103]) 28, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5R0YIsi008859 28, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 19:34:18 -0500 28, 23 -- X-ORBL: [99.154.21.201] 28, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 28, 23 -- by nlpi087.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id m5R0YGmG006585; 28, 23 -- Thu, 26 Jun 2008 19:34:17 -0500 28, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 28, 23 -- To: {Microscopy-at-microscopy.com} 28, 23 -- Cc: {rbeavers-at-mail.smu.edu} 28, 23 -- Subject: RE: [Microscopy] Tray for handling small specimens 28, 23 -- Date: Thu, 26 Jun 2008 17:34:33 -0700 28, 23 -- Message-ID: {00ef01c8d7ed$92d12460$7801a8c0-at-dynamicbl8uno3} 28, 23 -- MIME-Version: 1.0 28, 23 -- Content-Type: text/plain; 28, 23 -- charset="iso-8859-1" 28, 23 -- X-Mailer: Microsoft Office Outlook 11 28, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 28, 23 -- In-Reply-To: {200806262031.m5QKVOB9029337-at-ns.microscopy.com} 28, 23 -- Thread-Index: AcjXy5up2gZky5GvTPKdLVx11+pAggAHoJUQ 28, 23 -- Content-Transfer-Encoding: 8bit 28, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5R0YIsi008859 ==============================End of - Headers==============================
You might try a surplus outlet for electronics manufacturing equipment to obtain an inexpensive "vacuum tweezer" or die pick-and-place device. This consists of a tiny vacuum wand (shaped a bit like an airbrush sprayer) with a soft tip and a finger-controlled push button valve to release the vacuum. In its intended use, it is used to pick up a bare silicon chip (integrated circuit or "die") and set it down where it is to be bonded to a substrate. Tips are available that are small enough to handle a chip that is 20 thousandths of an inch square, or so. The vacuum can be provided by something as simple as an aquarium pump since the airflow is minimal.
John Twilley
} Group, } } Looking for some input for a small tray with side walls to catch small specimens dropped when observing under optical microscopes. } } I can make something for this application and got a pretty good idea from a local jeweler but hope there may be someone who has bought something commercially. } } This is in response to a student that lost a key thesis tooth fragment when it popped out of her tweezers while trying to place it under a microscope. } } Any ideas welcomed. } } Roy Beavers } Southern Methodist University } Department of Earth Sciences } P.O. Box 750395 } Dallas, TXÂ 75275 } Voice: 214-768-2756 } Fax: 214-768-2701 } Email: rbeavers-at-smu.edu } } } } ==============================Original Headers============================== } 8, 23 -- From rbeavers-at-mail.smu.edu Thu Jun 26 15:29:10 2008 } 8, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QKTAZ2023980 } 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 15:29:10 -0500 } 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 23 -- Thu, 26 Jun 2008 15:29:04 -0500 } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 23 -- Content-class: urn:content-classes:message } 8, 23 -- MIME-Version: 1.0 } 8, 23 -- Content-Type: text/plain; } 8, 23 -- charset="iso-8859-1" } 8, 23 -- Subject: Tray for handling small specimens } 8, 23 -- Date: Thu, 26 Jun 2008 15:29:03 -0500 } 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B304ABFCEB-at-s31xe7.systems.smu.edu} } 8, 23 -- X-MS-Has-Attach: } 8, 23 -- X-MS-TNEF-Correlator: } 8, 23 -- Thread-Topic: Tray for handling small specimens } 8, 23 -- Thread-Index: AcjXy0YrgkLekGoGT4y/bhWSb6TPDQ== } 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} } 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} } 8, 23 -- X-OriginalArrivalTime: 26 Jun 2008 20:29:04.0243 (UTC) FILETIME=[4692FC30:01C8D7CB] } 8, 23 -- Content-Transfer-Encoding: 8bit } 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QKTAZ2023980 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 27 -- From jtwilley-at-sprynet.com Thu Jun 26 20:03:14 2008 6, 27 -- Received: from elasmtp-spurfowl.atl.sa.earthlink.net (elasmtp-spurfowl.atl.sa.earthlink.net [209.86.89.66]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5R13ETr022252 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 20:03:14 -0500 6, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 27 -- s=dk20050327; d=sprynet.com; 6, 27 -- b=prZW6jjJBlQpOT++pexQoFchVkYQ8Id8LlEO+8ZyLxw78vqNbknSwiZq8Dx55EpO; 6, 27 -- h=Message-ID:Date:From:Reply-To:To:Subject:Cc:Mime-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-ELNK-Trace:X-Originating-IP; 6, 27 -- Received: from [209.86.224.37] (helo=elwamui-karabash.atl.sa.earthlink.net) 6, 27 -- by elasmtp-spurfowl.atl.sa.earthlink.net with esmtpa (Exim 4.67) 6, 27 -- (envelope-from {jtwilley-at-sprynet.com} ) 6, 27 -- id 1KC2Ms-00010O-1Q; Thu, 26 Jun 2008 21:03:14 -0400 6, 27 -- Received: from 70.23.77.145 by webmail.atl.earthlink.net with HTTP; Thu, 26 Jun 2008 21:03:13 -0400 6, 27 -- Message-ID: {32257414.1214528594052.JavaMail.root-at-elwamui-karabash.atl.sa.earthlink.net} 6, 27 -- Date: Thu, 26 Jun 2008 21:03:13 -0400 (EDT) 6, 27 -- From: jtwilley-at-sprynet.com 6, 27 -- Reply-To: jtwilley-at-sprynet.com 6, 27 -- To: rbeavers-at-mail.smu.edu 6, 27 -- Subject: Re: [Microscopy] Tray for handling small specimens 6, 27 -- Cc: Microscopy-at-microscopy.com 6, 27 -- Mime-Version: 1.0 6, 27 -- Content-Type: text/plain; charset=UTF-8 6, 27 -- X-Mailer: EarthLink Zoo Mail 1.0 6, 27 -- X-ELNK-Trace: adbb89a220ca650b5741bb2dafe82705a63b7957ab9b23b34f3dc13cfae53f45328cc31cc277dad5350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 6, 27 -- X-Originating-IP: 209.86.224.37 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5R13ETr022252 ==============================End of - Headers==============================
Erik, Contact M. E. Taylor at www.semsupplies.com . They will recoat and can also provide you with a second light pipe so you won't be down when a recoating is required. Actually this should probably be done 1-2 times per year if your usage is heavy.
Disclaimer: QI used to buy wholesale from Taylor and resell. We no longer do simply because our volume isn't very large. Many of our customers still buy directly from Taylor.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: mccullen-at-indigo.eng.wayne.edu [mailto:mccullen-at-indigo.eng.wayne.edu] Sent: Thursday, June 26, 2008 10:13 AM To: kenconverse-at-qualityimages.biz
Hello, The scintillator material on our Zeiss Leo 430 SEM is partially rubbed off. I've seen places selling p-47 scintillator disks for replacement. Can these be epoxyed onto the end of the light pipe, because the current material is directly coating the end of the light pipe? Or, do I need to have the pipe recoated or purchase a whole new pipe/scintillator combination?
Thanks, Erik
Dr. Erik McCullen Senior Research Scientist Smart Sensors and Integrated Microsystems Wayne State University 3164 Engineering BLDG. Detroit, MI 48202
Post Doctoral Position in the NCSU Analytical Instrumentation Facility FIB/TEM Laboratories
The NC State University Analytical Instrumentation Facility (AIF) has an opening for a postdoctoral research associate skilled in TEM and FIB (Hitachi HF-2000 Field Emission TEM and our FEI Quanta 3D Dual Beam FIB). The successful candidate must be a person who enjoys operating instrumentation, performing analyses and participating in the development and implementation of sample preparation and analytical techniques for a wide variety of samples (semiconductor, biological, polymer and ceramic, etc.). Additional training in TEM, FIB and other analytical techniques and instrumentation (AFM, SEM, XPS, SIMS, optical and stylus profilometry etc.) will be provided as needed for supporting TEM and FIB related research and analyses. For information on AIF instrumentation, see our web site: http://www.ncsu.edu/aif/.
A Ph.D. in Materials Science, Applied Physics, Analytical Chemistry, Biology or a related discipline with an in depth understanding of and hands on experience with operation of TEM instrumentation along with experience with FIB operation and FIB based TEM sample preparation techniques is required. Work in the laboratory will involve a combination of TEM/FIB analytical technique research, experimental design, instrument operation and data interpretation.
Please apply on line at http://jobs.ncsu.edu ( please search position # 04-31-0802). Applicants will need to submit a cover letter describing experience and interests, curriculum vitae and the names and addresses of three references. Review of applications will begin 07/18/2008, and this position will remain open until a suitable candidate is identified.
NC State University is an OEO/AA employer. NC State welcomes all persons without regard to sexual orientation. ADA individuals desiring reasonable accommodations call 919-515-3148.
==============================Original Headers============================== 8, 20 -- From dale_batchelor-at-ncsu.edu Fri Jun 27 09:04:00 2008 8, 20 -- Received: from uni13mr.unity.ncsu.edu (uni13mr.unity.ncsu.edu [152.1.224.171]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5RE3xrS003495 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2008 09:04:00 -0500 8, 20 -- Received: from [152.14.52.68] (dbmacx.aif.ncsu.edu [152.14.52.68]) 8, 20 -- by uni13mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2008.0610.1) with ESMTP id m5RE3udX021479 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jun 2008 10:03:58 -0400 (EDT) 8, 20 -- Mime-Version: 1.0 (Apple Message framework v624) 8, 20 -- Message-Id: {a8b32e96ec8c2ddc05bf84886b121760-at-ncsu.edu} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: Dale Batchelor {dale_batchelor-at-ncsu.edu} 8, 20 -- Subject: [Microscopy] Post Doc Position 8, 20 -- Date: Fri, 27 Jun 2008 10:04:37 -0400 8, 20 -- X-Mailer: Apple Mail (2.624) 8, 20 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.2.313940, Antispam-Data: 2008.6.27.134943 8, 20 -- X-Spam-Status: No, Hits=7% 8, 20 -- X-Spam-Level: IIIIIII 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5RE3xrS003495 ==============================End of - Headers==============================
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Thursday, June 26, 2008 3:31 PM To: Beavers, Roy
Group,
Looking for some input for a small tray with side walls to catch small specimens dropped when observing under optical microscopes.
I can make something for this application and got a pretty good idea from a local jeweler but hope there may be someone who has bought something commercially.
This is in response to a student that lost a key thesis tooth fragment when it popped out of her tweezers while trying to place it under a microscope.
Any ideas welcomed.
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 23 -- From rbeavers-at-mail.smu.edu Thu Jun 26 15:29:10 2008 8, 23 -- Received: from s31xub.systems.smu.edu (s31xub.systems.smu.edu [129.119.70.73]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5QKTAZ2023980 8, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jun 2008 15:29:10 -0500 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xub.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Thu, 26 Jun 2008 15:29:04 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="iso-8859-1" 8, 23 -- Subject: Tray for handling small specimens 8, 23 -- Date: Thu, 26 Jun 2008 15:29:03 -0500 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B304ABFCEB-at-s31xe7.systems.smu.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: Tray for handling small specimens 8, 23 -- Thread-Index: AcjXy0YrgkLekGoGT4y/bhWSb6TPDQ== 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 26 Jun 2008 20:29:04.0243 (UTC) FILETIME=[4692FC30:01C8D7CB] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5QKTAZ2023980 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 23 -- From rbeavers-at-mail.smu.edu Fri Jun 27 11:03:00 2008 19, 23 -- Received: from s31xua.systems.smu.edu (s31xua.systems.smu.edu [129.119.70.72]) 19, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5RG2wVP020705 19, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 27 Jun 2008 11:03:00 -0500 19, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xua.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 19, 23 -- Fri, 27 Jun 2008 11:02:57 -0500 19, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 23 -- Content-class: urn:content-classes:message 19, 23 -- MIME-Version: 1.0 19, 23 -- Content-Type: text/plain; 19, 23 -- charset="iso-8859-1" 19, 23 -- Subject: FW: [Microscopy] Tray for handling small specimens 19, 23 -- Date: Fri, 27 Jun 2008 11:02:57 -0500 19, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B304ABFE48-at-s31xe7.systems.smu.edu} 19, 23 -- X-MS-Has-Attach: 19, 23 -- X-MS-TNEF-Correlator: 19, 23 -- Thread-Topic: [Microscopy] Tray for handling small specimens 19, 23 -- Thread-Index: AcjXy5Y/ONu+9iBRSgeE3rjnT8TC5gAovPew 19, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 19, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 19, 23 -- X-OriginalArrivalTime: 27 Jun 2008 16:02:57.0738 (UTC) FILETIME=[44356AA0:01C8D86F] 19, 23 -- Content-Transfer-Encoding: 8bit 19, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m5RG2wVP020705 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both john.brealey-at-imvs.sa.gov.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: john.brealey-at-imvs.sa.gov.au Name: John Brealey
Organization: QEH
Title-Subject: [Filtered] Hitachi H-600 TEM Problems
Question: We have a Hitachi H-600 TEM that has served us well since 1983.
Friday I cleaned the anode and gave the gun chamber O-ring a very light application of fomblin grease. The machine was pumped down to vacuum and was operating normally (I could attain high vacuum and produce an electron beam on the screen). I turned it off for the weekend, as usual. Monday morning - no green high vacuum light in the gun chamber.
It appears the vacuum sequence works normally and all the valves that would be open during normal operation are open. The camera system (lower vacuum system) is OK and the green light for the camera is lit. The camera valve is the last valve to open - at this point the vacuum gauge for the gun moves from the far left of the gauge to the far right (I interpret this as the penning gauge switching on) however it fails to move from here. Both diffusion pumps are hot and produce the characteristic tinkling sound.
Possible cause...
1.Faulty penning gauge. 2.Poor vacuum in the gun chamber. 3.Faulty diffusion pump. 4.Faulty component on the high voltage stabilisation board. 5.Fuse 6.Power supply 7.Other?
Some things we've already tried...
1.Opened and closed the gun chamber twice plus inserted a new gun chamber O-ring. 2.Increased the temperature of the cooling water in case it was affecting the diffusion pump. 3.Tried to switch on the KV and view a beam on the screen. 4.Swapped the PB 42 EVACSEQ circuit board under the right console. 5.Swapped an integrated circuit on the HV stabilisation board (We had this problem 5 years ago and thought it may be the same fault). 6.Swapped the two rotary pumps between the camera and gun systems. 7.Took a blank photo.
The fact the all was fine on Friday suggests to me that something electrical has died on Monday. If there was a slow leak in the gun would we see any reading from the penning gauge? Is it worth swapping around the two diffusion pumps between camera and gun systems? Is it worth trying another penning gauge (I think I have a spare one)?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bobpasseri-at-ameritech.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bobpasseri-at-ameritech.net Name: Bob Passeri
Title-Subject: [Filtered] Hitachi H-600 TEM Problems
Question: Did you take off the Penning gauge and clean it? You can sonicate the metal loop in a 50:50 mixture of Micro-90 and water. It seems like to have power to the gauge because the scope is turning it on as the gauge moves from moves from left to right and stays there. I would clean the gauge and check for any shorts in the cableing.
A colleague of mine, Dennis O'Leary of MicroOptical Methods, is conducting a research project on why filters fail and is looking for samples (fluorescence cubes, polarizing filters, heat filters, etc.). If you have any sitting around the lab that you would like to contribute to his research, please get in touch with him: rdenol-at-hotmail.com or (518)482-8200. He is willing to pay for shipping.
Thanks in advance, Barbara Foster, President
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 E: bfoster-at-mme1.com W: www.MicroscopyEducation.com
NEWS! Our website, which had been down for nearly a month due to hackers, is back and better than ever. Come visit us at www.MicroscopyEducation.com! And Don't forget: MME is now scheduling customized, on-site courses through Dec 2008. Call me for a free assessment and quote.
==============================Original Headers============================== 7, 21 -- From bfostermme-at-sbcglobal.net Mon Jun 30 10:34:19 2008 7, 21 -- Received: from smtp111.sbc.mail.mud.yahoo.com (smtp111.sbc.mail.mud.yahoo.com [68.142.198.210]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m5UFYJCd029283 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jun 2008 10:34:19 -0500 7, 21 -- Message-Id: {200806301534.m5UFYJCd029283-at-ns.microscopy.com} 7, 21 -- Received: (qmail 53377 invoked from network); 30 Jun 2008 15:34:18 -0000 7, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 21 -- s=s1024; d=sbcglobal.net; 7, 21 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:X-Mailer:Date:To:From:Subject:Mime-Version:Content-Type; 7, 21 -- b=zoSjf0Df4dwPdpo5cIaF4DQ1xbZ8S6hu55W+Lw/dsteGhM7TKYvgXbDI5LAtaaGKVz88JzmhkhPQ1EdcYmbYrGbUPAjZfB8vjgChC+caEbgjxAXFAtjTcEX0EVJl1wb4wB9DgkIn1U7hpCUNbcQ9orMhLTEVcAERfUMrGQM5a6g= ; 7, 21 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-sbcglobal.net-at-68.94.34.245 with login) 7, 21 -- by smtp111.sbc.mail.mud.yahoo.com with SMTP; 30 Jun 2008 15:34:18 -0000 7, 21 -- X-YMail-OSG: ZRdtNXcVM1mtyPJSd9v0TWBA6bSHsIRHWP2v2i8Xmn_V3FnejvRhNRxEoMqSnV_gcEZSfuNv.CATh9eLrm29O75nAGWg2AAtuw52yc1GIVD3MvlmB.ekjb.Dyn3D3deIZ7stQb1W3dOqGgVSHnoLbgnn 7, 21 -- X-Yahoo-Newman-Property: ymail-3 7, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 21 -- Date: Mon, 30 Jun 2008 10:34:05 -0500 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 7, 21 -- Subject: Reasons why filters fail 7, 21 -- Mime-Version: 1.0 7, 21 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Here is the July 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Friday July 3rd, 2008. Sorry for the short notice--it is due to the requirement to have the magazine printed well before the M&M meeting in Albuquerque.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor
========================= How Much Force Does it Take to Move an Atom on a Surface? Stephen W. Carmichael, Mayo Clinic
Beyond the Black Box: Interactive Global Docking of Protein Complexes Jochen Heyd, Stefan Birmanns, University of Texas at Houston, School of Health Information Sciences, Houston, Texas
Comparing Binary Image Analysis Measurements – Euclidean Geometry, Centroids and Corners Dennis W. Hetzner, Timken, Co. Canton, Ohio
Confocal Laser Microscopy on Biofilms: Successes and Limitations Betsey Pitts and Philip Stewart, Center for Biofilm Engineering, Montana State University, Bozeman, Montana
Extreme High-Resolution SEM: A Paradigm Shift R. Young*, T. Templeton*, L. Roussel**, I. Gestmann**, G. van Veen**, T. Dingle** and S. Henstra** FEI, *Hillsboro, OR and **Eindhoven, The Netherlands
Testing Parameters for Two-Dimensional Crystallization and Electron Crystallography on Eukaryotic Membrane Proteins with Liposomes as Controls G. Zhaoa, V. Mutucumaranab, D. Staffordb, Y. Kanaokac, K. F. Austenc and I. Schmidt-Kreya ,GA Inst. of Tech., Atlanta, UNC, Chapel Hill, NC, Harvard and Brigham and Women’s Boston, MA
A New Perspective on Mechanical Testing: In Situ Compression in the TEM Julia Deneen Nowak, Zhiwei Shan and Oden L. Warren,*Hysitron Incorporated, Minneapolis, MN
Confocal Micro X-Ray Fluorescence: A New Paradigm in Materials Characterization Brian M. Patterson, George J. Havrilla, Kimberly A. DeFriend, LANL, Los Alamos, NM
Hardware and Techniques for Cross-Correlative TEM and Atom Probe Analysis B.P. Gorman,1 D. Diercks,1 N. Salmon,2 E. Stach,2 G. Amador,3 C. Hartfield,3 1U. of North Texas, Denton, TX, 2Hummingbird Scientific Instruments, Salem, OR,3Omniprobe, Inc., Dallas, TX
Imaging Skin Epidermal Stem Cells: A Review Hilda Amalia Pasolli, The Rockefeller University New York, NY
Signature Analysis Applied to EDS Microanalysis J.W. Colby & D. C. Ward, xk, Incorporated, Clackamas, OR
Cryo-Fracture or Freeze-Fracture, a Method to Expose Internal Tissue Surfaces and Cell Surfaces for Viewing in the Scanning Electron Microscope Jeannette Taylor, Emory University, Atlanta, Georgia
SEM Remote Control with a 3D Option F. Mighela, C. Perra, R. Pintus, S. Podda, M. Vanzi, Sardegna Ricerche, Pula, Italy and DIEE, U. of Cagliari, Cagliari, Italy
Coloring Pictures for Electron Microscopists or Elements of Digital Image Manipulation for Students V.M.Dusevich, J.H.Purk, J.D.Eick, University of Missouri, Kansas City, MO
Industry News
NetNotes SPECIMEN PREPARATION – acetonitrile as a dehydration agent SPECIMEN PREPARATION - wicking or blotting grids SPECIMEN PREPARATION - sample mounting SPECIMEN PREPARATION – drying carbon paint for SEM SPECIMEN PREPARATION – dispersing particle for SEM SPECIMEN PREPARATION - sputter coating SPECIMEN PREPARATION – coating for SEM SPECIMEN PREPARATION – evaporating thick Al layers IMMUNOCYTOCHEMISTRY – brain tissue for EM DIGITAL IMAGING – resolution EM - algae in chiller EM - maximum length of vacuum lines EM - magnetic interference from UPS EM - filament life TEM - FFTs in focusing and removing objective astigmatism SEM - low keV imaging SEM - interference SEM – digital camera EDX problems
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==============================Original Headers============================== 24, 17 -- From randerson20-at-tampabay.rr.com Mon Jun 30 11:59:36 2008 24, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123]) 24, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m5UGxaje013101 24, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 30 Jun 2008 11:59:36 -0500 24, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 24, 17 -- by hrndva-omta05.mail.rr.com with ESMTP 24, 17 -- id {20080630165936.YGAA5951.hrndva-omta05.mail.rr.com-at-[127.0.0.1]} 24, 17 -- for {Microscopy-at-Microscopy.Com} ; Mon, 30 Jun 2008 16:59:36 +0000 24, 17 -- Message-ID: {486910F0.1010000-at-tampabay.rr.com} 24, 17 -- Date: Mon, 30 Jun 2008 12:59:28 -0400 24, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 24, 17 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 24, 17 -- MIME-Version: 1.0 24, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 24, 17 -- Subject: July 2008 Microscopy Today Table of Contents 24, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 24, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: qxing-at-ameslab.gov Name: Qingfeng Xing
Organization: Ames Laboratory
Title-Subject: [Filtered] Detector of X-ray Back-Reflection Laue
Question: Dear all:
Iíd like to know whether anyone could give me some information on an image plate or CCD detector for X-ray Back-reflection Laue.
We are currently using Polaroid plates (4*5) for our Philips PW-1830 diffractometer. We are planning to put a detector of image plate or CCD on the diffractometer. Our work requires that the detector size is no less than about 9cm*11.5cm. Any information of maker, price and so on would be appreciated.
Thanks to Joel McClintock and Bob Passeri for kind advice on this problem. Your advice convinced me the problem was with the penning gauge so I attempted something very scientific to try to fix it - I hit it with a ruler! And voila, we have a green light on the high vacuum gauge. TEM now fully operational. When things back to normal we will have to look into cleaning the penning gauge.
Thanks again, John Brealey
John,
Wow. You really attacked this. Common occurance on a H-600 is a dirty Penning gauge. Remember a Penning gauge acts like a pump and grabs contaminants. It shorts out due to carbon build up. Sometimes when the gauge reaches the point in the vacuum sequence to come on it will go all the way right and then not move, sometimes it will go right and waiver up and then back erratically. That symptom is not always the penning but usually. There are a couple clues to look for as I suspect you were right in your initial suspisions. When pumping the gun down From atmosphere to when the penning comes on, does the gun pirani act normal? Take a reasonable amount of time to reach this point? Clean and/or swap the penning. By the way I would suggest not putting any grease on the o'rings in and around the penning gauge as it will make the gauge dirty quick.
It is not anything to do with the HV or HV stabilizer circuit. In the flow of operating the H-600 needs a column green light for HV to even come on. Almost certainly not the DP. Don't think it is the roughing pump either.
Oh, if you take an ohmeter and measure the resistance from where the plug is on the penning gauge to anywhere on the metal of the penning gauge and read a short or low resistance, it is shorted causing your problem. Only do this if the gun is at air or EVAC off as there is ~800VDC at the penning plug when on. If it is not a short it may be when the voltage is on. CLEAN IT!!!
Good luck
Joel
Title-Subject: [Filtered] Hitachi H-600 TEM Problems
Question: We have a Hitachi H-600 TEM that has served us well since 1983.
Friday I cleaned the anode and gave the gun chamber O-ring a very light application of fomblin grease. The machine was pumped down to vacuum and was operating normally (I could attain high vacuum and produce an electron beam on the screen). I turned it off for the weekend, as usual. Monday morning - no green high vacuum light in the gun chamber.
It appears the vacuum sequence works normally and all the valves that would be open during normal operation are open. The camera system (lower vacuum system) is OK and the green light for the camera is lit. The camera valve is the last valve to open - at this point the vacuum gauge for the gun moves from the far left of the gauge to the far right (I interpret this as the penning gauge switching on) however it fails to move from here. Both diffusion pumps are hot and produce the characteristic tinkling sound.
Possible cause...
1.Faulty penning gauge. 2.Poor vacuum in the gun chamber. 3.Faulty diffusion pump. 4.Faulty component on the high voltage stabilisation board. 5.Fuse 6.Power supply 7.Other?
Some things we've already tried...
1.Opened and closed the gun chamber twice plus inserted a new gun chamber O-ring. 2.Increased the temperature of the cooling water in case it was affecting the diffusion pump. 3.Tried to switch on the KV and view a beam on the screen. 4.Swapped the PB 42 EVACSEQ circuit board under the right console. 5.Swapped an integrated circuit on the HV stabilisation board (We had this problem 5 years ago and thought it may be the same fault). 6.Swapped the two rotary pumps between the camera and gun systems. 7.Took a blank photo.
The fact the all was fine on Friday suggests to me that something electrical has died on Monday. If there was a slow leak in the gun would we see any reading from the penning gauge? Is it worth swapping around the two diffusion pumps between camera and gun systems? Is it worth trying another penning gauge (I think I have a spare one)?
Any assistance gratefully accepted.
Regards,
John Brealey
EM Unit
Queen Elizabeth Hospital
Adelaide
South Australia
==============================Original Headers============================== 30, 35 -- From john.brealey-at-imvs.sa.gov.au Tue Jul 1 02:25:09 2008 30, 35 -- Received: from adl0741.systems.sa.gov.au (adl0741.systems.sa.gov.au [143.216.236.20]) 30, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m617P8o8009922 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 02:25:09 -0500 30, 35 -- Received: from adl0741.systems.sa.gov.au (localhost [127.0.0.1]) 30, 35 -- by adl0741.systems.sa.gov.au OUTGOING (8.12.10/8.12.10) with ESMTP id m617P6iR000262 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 16:55:07 +0930 (CST)' 30, 35 -- Received: from ablett.imvs.sa.gov.au (ablett.imvs.sa.gov.au [10.20.98.41]) 30, 35 -- by adl0741.systems.sa.gov.au INCOMING (8.12.10/8.12.10) with ESMTP id m617P6XF000229 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 16:55:06 +0930 (CST)' 30, 35 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) 30, 35 -- by ablett.imvs.sa.gov.au (Postfix) with ESMTP id 686602EC0CA 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 16:55:06 +0930 (CST) 30, 35 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 30, 35 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) 30, 35 -- by localhost (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) 30, 35 -- with LMTP id 8+DtFpmZZzA3 for {Microscopy-at-microscopy.com} ; 30, 35 -- Tue, 1 Jul 2008 16:54:52 +0930 (CST) 30, 35 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) 30, 35 -- by ablett.imvs.sa.gov.au (Postfix) with SMTP id 3BA1C35072 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 16:54:52 +0930 (CST) 30, 35 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 30, 35 -- From: "John BREALEY" {john.brealey-at-imvs.sa.gov.au} 30, 35 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 30, 35 -- Subject: Hitachi H-600 now fixed 30, 35 -- Date: Tue, 1 Jul 2008 16:54:51 +0930 30, 35 -- Organization: IMVS 30, 35 -- Message-ID: {000301c8db4b$8dc7f0a0$7d8a140a-at-41347i} 30, 35 -- MIME-Version: 1.0 30, 35 -- Content-Type: text/plain; 30, 35 -- charset="us-ascii" 30, 35 -- Content-Transfer-Encoding: 7bit 30, 35 -- X-Mailer: Microsoft Office Outlook 11 30, 35 -- Thread-Index: AcjbS40V/6ZKeBabRtmepWE00iX57w== 30, 35 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
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Candidates should have an interest in structural biology and virology, and should enjoy working as part of an energetic team. Background experience in one (or more) of the following fields is desirable, but not required: virology, (cryo-) electron microscopy, cryo-sectioning (frozen-hydrated), and/or image processing/analysis techniques.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both microbill-at-moahwk.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: microbill-at-moahwk.net Name: BILL MILLER
Organization: ElectroImage
Title-Subject: [Filtered] Imaging plates for Laue X-ray systems
Question: Dr. Qingfeng - the Ditabis imaging plate system (http://www.ditabis.de or http://www.electroimage.com), although made for electron imaging, can be and has been used for X-ray Back-Reflection Laue as well. As a disclaimer - ElectroImage is the US distributor for Ditabis.
Thanks very much to all who responded. I really appreciate your input (as always). Everyone offered pretty much the same point of view, which is somewhat different from my initial thinking, and so your comments have been extremely valuable as we go about inventing our own wheel here.
Ann H. Lehman Electron Microscopy Facility at Trinity College 300 Summit Street Hartford CT 06106 v. 860-297-4289 f. 860-297-2538 e. ann.lehman-at-trincoll.edu w. http://www.trincoll.edu/~alehman
________________________________
X-from: Lehman, Ann R Sent: Wed 6/25/2008 5:58 AM To: Microscopy-at-Microscopy.com
Can anyone tell me a supplier for the stackable Fluoroware(R) wafer containers. These are the containers that have a little star-shaped unit in them to keep a wafer from bouncing around. They come in different sizes. They have the left-hand opening lids. I can't seem to find a source for them and my limited supply here is just about gone.
Thanks in advance.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
==============================Original Headers============================== 4, 20 -- From walck-at-southbaytech.com Tue Jul 1 20:01:32 2008 4, 20 -- Received: from nlpi087.prodigy.net (nlpi087.prodigy.net [207.115.36.103]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6211Vvx012311 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 20:01:32 -0500 4, 20 -- X-ORBL: [99.154.21.201] 4, 20 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 4, 20 -- by nlpi087.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id m6211V7H029944 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Jul 2008 20:01:31 -0500 4, 20 -- From: "Scott Walck" {walck-at-southbaytech.com} 4, 20 -- To: {Microscopy-at-microscopy.com} 4, 20 -- Subject: Fluroware(R) container suppliers 4, 20 -- Date: Tue, 1 Jul 2008 18:01:37 -0700 4, 20 -- Message-ID: {00ca01c8dbdf$2e844730$7801a8c0-at-dynamicbl8uno3} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: text/plain; 4, 20 -- charset="US-ASCII" 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-Mailer: Microsoft Office Outlook 11 4, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 4, 20 -- Thread-Index: Acjb3y3wDwAmTiu/QnyWxLKJNABGmQ== ==============================End of - Headers==============================
Disclaimer: I have not dealt with wafercare.com; my clients have brought me samples in these containers and i like them. I never asked where they got the containers. Google is my friend {grin} .
Cheers, John Minter
==============================Original Headers============================== 7, 19 -- From jrminter-at-rochester.rr.com Wed Jul 2 08:15:06 2008 7, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m62DF6pC029049 7, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 08:15:06 -0500 7, 19 -- Received: from hrndva-web02-z01 ([10.128.132.93]) 7, 19 -- by hrndva-smta01.mail.rr.com with ESMTP 7, 19 -- id {20080702131505.GBPV3927.hrndva-smta01.mail.rr.com-at-hrndva-web02-z01} 7, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 13:15:05 +0000 7, 19 -- Message-ID: {31501241.272091215004506043.JavaMail.root-at-hrndva-web02-z01} 7, 19 -- Date: Wed, 2 Jul 2008 9:15:05 -0400 7, 19 -- From: {jrminter-at-rochester.rr.com} 7, 19 -- To: Microscopy-at-microscopy.com 7, 19 -- Subject: Re: [Microscopy] Fluroware containers 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=utf-8 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- X-Priority: 3 (Normal) 7, 19 -- Sensitivity: Normal 7, 19 -- X-Originating-IP: from 67.240.238.57 by webmail.rochester.rr.com; Wed, 2 Jul 2008 13:15:05 +0000 ==============================End of - Headers==============================
The safety folks here are starting to give the evil eye to the propylene oxide. In the past I've skipped the po as an intermediate and used ETOH or Acetone in the infiltration steps.
What is the consensus, or is there one? The less toxic/flammable the better.
Thanks in advance,
Paula :-)
Paula Sicurello VA San Diego Healthcare System Microscope Facility room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
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I am conducting a retained search on behalf of a major pre-IPO nanotechnology startup with substantial funding, a very large IP portfolio, and under 100 employees. The position is based in Northern Silicon Valley and the company is doing some exciting research in areas like printed, flexible electronics, solar cells, power supplies, gene-assay systems and nanocrystal memory. The company offers a competitive package including benefits and stock options. If you are interested or have referrals or suggestions, please send me a resume or alert me.
Thank You,
Nick Meyler
GM/President, Technology Division
Wingate Dunross Associates, Inc
ph (818)597-3200 ext. 211
email: "nickm(at)wdsearch.com"
Electron Microscopist:
My client seeks a highly skilled Electron Microscopist. In this role you will use transmission (TEM) and scanning electron microscopy (SEM) to evaluate and contribute to nanotechnology materials development and process monitoring at the company. Responsibilities include measuring a wide variety of materials and devices, preparing samples, tracking processes and communicating findings to accelerate development. In addition, you will be expected to streamline their microscopy methodologies. You will be a regular participant in group meetings and present your results and insights. As available, you will assist in other projects and develop expertise in other characterization tools.
Requirements: B.S. or equivalent in Materials Science or related field; 3+ years industrial experience; experience in preparing cross-section samples for failure analysis; excellent oral and written communication skills
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If you look at nice work that has been published and take the minimalist stance, then you have to accept that acetone is perfectly capable of yielding beautiful results when used as the dehydrating and infiltration medium for epoxy resins. I think I read in Hayat that it is less hygroscopic than ethanol and has some other benefits. There may be some specific cases where propylene oxide or another solvent/combination could be better, but I have seen enough to believe that acetone is probably a perfectly good solvent in almost all cases.
Along these same minimalist lines, I have seen enough excellent work using room temperature dehydrations that I wouldn't routinely bother with dehydrations in the cold except for very delicate samples. Here there is a slightly better case for the trouble of cold dehydration, since most materials become stiffer at lower temps and it may protect from some tissue distortions.
My advice is probably worth exactly what you paid,
Dale Callaham
vapatpxs-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi listers, } } The safety folks here are starting to give the evil eye to the propylene oxide. In the past I've skipped the po as an intermediate and used ETOH or Acetone in the infiltration steps. } } What is the consensus, or is there one? The less toxic/flammable the better. } } Thanks in advance, } } Paula :-) } } Paula Sicurello } VA San Diego Healthcare System } Microscope Facility room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } } } } ==============================Original Headers============================== } 9, 24 -- From vapatpxs-at-yahoo.com Wed Jul 2 12:05:55 2008 } 9, 24 -- Received: from n55.bullet.mail.sp1.yahoo.com (n55.bullet.mail.sp1.yahoo.com [98.136.44.188]) } 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m62H5t6q024833 } 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 12:05:55 -0500 } 9, 24 -- Received: from [216.252.122.216] by n55.bullet.mail.sp1.yahoo.com with NNFMP; 02 Jul 2008 17:05:54 -0000 } 9, 24 -- Received: from [69.147.65.158] by t1.bullet.sp1.yahoo.com with NNFMP; 02 Jul 2008 17:05:54 -0000 } 9, 24 -- Received: from [127.0.0.1] by omp406.mail.sp1.yahoo.com with NNFMP; 02 Jul 2008 17:05:54 -0000 } 9, 24 -- X-Yahoo-Newman-Property: ymail-3 } 9, 24 -- X-Yahoo-Newman-Id: 798689.44215.bm-at-omp406.mail.sp1.yahoo.com } 9, 24 -- Received: (qmail 78455 invoked by uid 60001); 2 Jul 2008 17:05:54 -0000 } 9, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 9, 24 -- s=s1024; d=yahoo.com; } 9, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; } 9, 24 -- b=C8Fujo52NLt6SW2+xdE8LasZ4kFBzDl98lPxRd64LL399M/pSOC+FMq1Eq96+ijDc8GUlUr+6iraFIr0oWUvEUEFF8WvZbUlmuevOWuK8fC0w1bG+yXY+8bOhfhdj60hktvalh+7k1NgVFo8IPpwiCT/kQ2i60/UZxlh64DCYtA=; } 9, 24 -- Received: from [132.239.85.200] by web46108.mail.sp1.yahoo.com via HTTP; Wed, 02 Jul 2008 10:05:54 PDT } 9, 24 -- X-Mailer: YahooMailWebService/0.7.199 } 9, 24 -- Date: Wed, 2 Jul 2008 10:05:54 -0700 (PDT) } 9, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} } 9, 24 -- Reply-To: vapatpxs-at-yahoo.com } 9, 24 -- Subject: Propylene Oxide vs Ethanol or Acetone } 9, 24 -- To: MSA BB {Microscopy-at-microscopy.com} } 9, 24 -- MIME-Version: 1.0 } 9, 24 -- Content-Type: text/plain; charset=us-ascii } 9, 24 -- Message-ID: {498337.78015.qm-at-web46108.mail.sp1.yahoo.com} } ==============================End of - Headers==============================
I don't know the reference, but I recall being told that one advantage of using propylene oxide is that the molecule gets incorporated into the polymerized resin. This is not so for other solvents, which can lead to localized regions of poorly polymerized resin. If you are careful, acetone and ethanol should work fine.
--John
John Chandler Colorado School of Mines jpchandl-at-mines.edu 303.384.2203 (office)
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Wednesday, July 02, 2008 1:58 PM To: jpchandl-at-mines.edu
Hi Paula,
If you look at nice work that has been published and take the minimalist stance, then you have to accept that acetone is perfectly capable of yielding beautiful results when used as the dehydrating and infiltration medium for epoxy resins. I think I read in Hayat that it is less hygroscopic than ethanol and has some other benefits. There may be some specific cases where propylene oxide or another solvent/combination could be better, but I have seen enough to believe that acetone is probably a perfectly good solvent in almost all cases.
Along these same minimalist lines, I have seen enough excellent work using room temperature dehydrations that I wouldn't routinely bother with dehydrations in the cold except for very delicate samples. Here there is a slightly better case for the trouble of cold dehydration, since most materials become stiffer at lower temps and it may protect from some tissue distortions.
My advice is probably worth exactly what you paid,
Dale Callaham
vapatpxs-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi listers, } } The safety folks here are starting to give the evil eye to the propylene oxide. In the past I've skipped the po as an intermediate and used ETOH or Acetone in the infiltration steps. } } What is the consensus, or is there one? The less toxic/flammable the better. } } Thanks in advance, } } Paula :-) } } Paula Sicurello } VA San Diego Healthcare System } Microscope Facility room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397
==============================Original Headers============================== 18, 21 -- From jpchandl-at-mines.edu Wed Jul 2 15:44:24 2008 18, 21 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU [138.67.130.5]) 18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m62KiOeR007960 18, 21 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 15:44:24 -0500 18, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 18, 21 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id m62KiKCq004535; 18, 21 -- Wed, 2 Jul 2008 14:44:23 -0600 18, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} 18, 21 -- To: {dac-at-research.umass.edu} , {microscopy-at-microscopy.com} 18, 21 -- References: {200807021957.m62Jvi85001469-at-ns.microscopy.com} 18, 21 -- Subject: RE: [Microscopy] Re: Propylene Oxide vs Ethanol or Acetone 18, 21 -- Date: Wed, 2 Jul 2008 14:44:20 -0600 18, 21 -- Message-ID: {002101c8dc84$6746dbf0$ad1c438a-at-mines.edu} 18, 21 -- MIME-Version: 1.0 18, 21 -- Content-Type: text/plain; 18, 21 -- charset="us-ascii" 18, 21 -- Content-Transfer-Encoding: 7bit 18, 21 -- X-Mailer: Microsoft Office Outlook 11 18, 21 -- In-Reply-To: {200807021957.m62Jvi85001469-at-ns.microscopy.com} 18, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 18, 21 -- Thread-Index: AcjcfeWIUj7F6RZBQ1Srr7gE00n2uwABdEpg ==============================End of - Headers==============================
The FOM FIG, Facility Operation and Management Focus Interest Group, will be meeting for a Round Table Lunch Discussion on Tuesday August 5 at M&M 2008.
The topics for discussion will be chosen from the following list: 1. How to make microscopy relevant (and essential) to modern scientific problems. 2. "Full service EM" - good, bad, how to bill, what to provide, acknowledgments etc. 3. Strategies for obtaining funding for major instrumentation.
Please list these topics in order of interest to you - by number - in your reply.
A boxed lunch will be provided for current members of the FOM FIG. Non-members may join the FOM FIG prior to the meeting by going through the MSA website ( http://www.microscopy.org/). Non-FOM FIG members will be required to pay $25.00 at the door.
The boxed lunch will contain MONZANO WRAPs: Deli-Style Smoked Turkey, Ham, Roast Beef, or Grilled Vegetables, wrapped in a Jumbo-Flour Tortilla with Lettuce, Tomato and Pesto Cream Cheese Spread served with Chef's Choice of Seasonal Fresh Fruit, and Carrot Cake.
We need to know how many lunches to order and we need this information by Wednesday night, July 9.
Since I will be unavailable for e-mail after July 3, for possibly 10 days, please send your intention of joining the discussion to the FOM FIG secretary, Pat Connelly at psconnelly-at-gmail.com
We hope to see you in Albuquerque! Elaine
Dr. Elaine Humphrey Microscopy Specialist MSA FOM FIG Leader (2006-8)
==============================Original Headers============================== 9, 24 -- From connellyps-at-nhlbi.nih.gov Wed Jul 2 17:49:22 2008 9, 24 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m62MnMpm024782 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 17:49:22 -0500 9, 24 -- X-IronPortListener: Outbound_SMTP 9, 24 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 9, 24 -- by nihxway4out.hub.nih.gov with ESMTP; 02 Jul 2008 18:49:22 -0400 9, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 24 -- Wed, 2 Jul 2008 18:49:11 -0400 9, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.166]) with Microsoft Exchange Server HTTP-DAV ; 9, 24 -- Wed, 2 Jul 2008 22:49:10 +0000 9, 24 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 24 -- Date: Wed, 02 Jul 2008 18:45:18 -0400 9, 24 -- Subject: Facility Operation and Management Focus Interest Group 9, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 24 -- Message-ID: {C4917D3E.1FD4%connellyps-at-nhlbi.nih.gov} 9, 24 -- Thread-Topic: Facility Operation and Management Focus Interest Group 9, 24 -- Thread-Index: AcjclUz9i44GB0iIEd2OZQANk2Yv1A== 9, 24 -- Mime-version: 1.0 9, 24 -- Content-type: text/plain; 9, 24 -- charset="US-ASCII" 9, 24 -- Content-transfer-encoding: 7bit 9, 24 -- X-OriginalArrivalTime: 02 Jul 2008 22:49:11.0166 (UTC) FILETIME=[D7F821E0:01C8DC95] ==============================End of - Headers==============================
If someone has that reference, I'd be interested. I used to believe that the advantage of PO is that it evaporates without a trace - which is easy to believe for anyone who has ever handled PO.
We are fortunate here, noone tells us what to use or not use for dehydration and infiltration. PO gives you a piece of mind, all my infiltration problems disappeared and never came back since I started using PO, 9 years ago.
With all due respect, Hyatt books (I have almost all) are really a vast collection of recipes and anecdotes, many of which contradict each other. I wouldn't refer to them as a bible. There are much better sources these days, like Bozzola/Russel, Afzelius/Maunsbach...
For epon analogs, my understanding is one should use at least acetone, ethanol alone won't do. I have never tried going from ethanol straight to epon but have noticed that when the aceton is not freshly opened, I often have infiltration issues. I would use those small bottles of glass-distilled acetone sold by EM vendors and open a fresh bottle every time.
A few teaching EM labs I've ben to use molecular sieves in bottles of pure ethanol and acetone to keep them dry. Reportedly, this can cause problems with diamond knives...
It all depends on what you must infiltrate, of course. While you can do just fine w/o PO with mammalian cell culture and many tissues, skin will be more difficult.
Best regards, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} From: jpchandl-at-mines.edu } Date: July 2, 2008 4:45:12 PM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] Propylene Oxide vs Ethanol or Acetone } Reply-To: jpchandl-at-mines.edu } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Paula, } } I don't know the reference, but I recall being told that one } advantage of } using propylene oxide is that the molecule gets incorporated into the } polymerized resin. This is not so for other solvents, which can } lead to } localized regions of poorly polymerized resin. If you are careful, } acetone } and ethanol should work fine. } } --John } } John Chandler } Colorado School of Mines } jpchandl-at-mines.edu } 303.384.2203 (office) } } } -----Original Message----- } X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] } Sent: Wednesday, July 02, 2008 1:58 PM } To: jpchandl-at-mines.edu } Subject: [Microscopy] Re: Propylene Oxide vs Ethanol or Acetone } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Paula, } } If you look at nice work that has been published and take the } minimalist } stance, then you have to accept that acetone is perfectly capable of } yielding beautiful results when used as the dehydrating and } infiltration } medium for epoxy resins. I think I read in Hayat that it is less } hygroscopic than ethanol and has some other benefits. There may be } some } specific cases where propylene oxide or another solvent/combination } could be better, but I have seen enough to believe that acetone is } probably a perfectly good solvent in almost all cases. } } Along these same minimalist lines, I have seen enough excellent work } using room temperature dehydrations that I wouldn't routinely bother } with dehydrations in the cold except for very delicate samples. Here } there is a slightly better case for the trouble of cold dehydration, } since most materials become stiffer at lower temps and it may protect } from some tissue distortions. } } My advice is probably worth exactly what you paid, } } Dale Callaham } } vapatpxs-at-yahoo.com wrote: } } } ---------------------------------------------------------------------- } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } ------ } } } } Hi listers, } } } } The safety folks here are starting to give the evil eye to the } } propylene } oxide. In the past I've skipped the po as an intermediate and used } ETOH or } Acetone in the infiltration steps. } } } } What is the consensus, or is there one? The less toxic/flammable the } better. } } } } Thanks in advance, } } } } Paula :-) } } } } Paula Sicurello } } VA San Diego Healthcare System } } Microscope Facility room B141 } } 3350 La Jolla Village Dr., MC151 } } San Diego, CA 92161 } } 858-552-8585 x2397 } } } } } ==============================Original } Headers============================== } 18, 21 -- From jpchandl-at-mines.edu Wed Jul 2 15:44:24 2008 } 18, 21 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU } [138.67.130.5]) } 18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m62KiOeR007960 } 18, 21 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 } 15:44:24 -0500 } 18, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) } 18, 21 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id } m62KiKCq004535; } 18, 21 -- Wed, 2 Jul 2008 14:44:23 -0600 } 18, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} } 18, 21 -- To: {dac-at-research.umass.edu} , {microscopy-at-microscopy.com} } 18, 21 -- References: {200807021957.m62Jvi85001469-at-ns.microscopy.com} } 18, 21 -- Subject: RE: [Microscopy] Re: Propylene Oxide vs Ethanol } or Acetone } 18, 21 -- Date: Wed, 2 Jul 2008 14:44:20 -0600 } 18, 21 -- Message-ID: {002101c8dc84$6746dbf0$ad1c438a-at-mines.edu} } 18, 21 -- MIME-Version: 1.0 } 18, 21 -- Content-Type: text/plain; } 18, 21 -- charset="us-ascii" } 18, 21 -- Content-Transfer-Encoding: 7bit } 18, 21 -- X-Mailer: Microsoft Office Outlook 11 } 18, 21 -- In-Reply-To: {200807021957.m62Jvi85001469-at-ns.microscopy.com} } 18, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 18, 21 -- Thread-Index: AcjcfeWIUj7F6RZBQ1Srr7gE00n2uwABdEpg } ==============================End of - } Headers==============================
==============================Original Headers============================== 12, 23 -- From vladislav_speransky-at-nih.gov Wed Jul 2 18:22:54 2008 12, 23 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m62NMsU2006382 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 18:22:54 -0500 12, 23 -- X-IronPortListener: NIH_Relay 12, 23 -- X-SBRS: None 12, 23 -- X-IronPort-AV: E=Sophos;i="4.27,739,1204520400"; 12, 23 -- d="scan'208";a="19729504" 12, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 12, 23 -- by nihrelayxway3.hub.nih.gov with ESMTP; 02 Jul 2008 19:22:54 -0400 12, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 12, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m62NMnRN029656 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 19:22:52 -0400 12, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 12, 23 -- References: {200807022045.m62KjC5w009313-at-ns.microscopy.com} 12, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 23 -- Message-Id: {B608A7D4-80AE-4C07-8E5B-507DADB89519-at-nih.gov} 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 12, 23 -- Subject: Fwd: [Microscopy] Propylene Oxide vs Ethanol or Acetone 12, 23 -- Date: Wed, 2 Jul 2008 19:22:08 -0400 12, 23 -- To: Microscopy-at-microscopy.com 12, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Some questions have been raised on this position, and I need to add a little bit more information. Firstly, the position is for someone with a BS and not a PhD; this is partly a job-duty issue and partly a salary issue. The projected compensation for this position is above $60K, but definitely not close to six figures.
Also, I am only interested in candidates with commercial experience (3+ years) -- no fresh graduates, typically.
Finally, if you are an applicant from outside of California, be aware that this will involve relocation to Silicon Valley. California candidates are very much preferred.
Your comments or feedback are very welcome on these points, of course.
I am conducting a retained search on behalf of a major pre-IPO nanotechnology startup with substantial funding, a very large IP portfolio, and under 100 employees. The position is based in Northern Silicon Valley and the company is doing some exciting research in areas like printed, flexible electronics, solar cells, power supplies, gene-assay systems and nanocrystal memory. The company offers a competitive package including benefits and stock options. If you are interested or have referrals or suggestions, please send me a resume or alert me.
My client seeks a highly skilled Electron Microscopist. In this role you will use transmission (TEM) and scanning electron microscopy (SEM) to evaluate and contribute to nanotechnology materials development and process monitoring at the company. Responsibilities include measuring a wide variety of materials and devices, preparing samples, tracking processes and communicating findings to accelerate development. In addition, you will be expected to streamline their microscopy methodologies. You will be a regular participant in group meetings and present your results and insights. As available, you will assist in other projects and develop expertise in other characterization tools.
Requirements: B.S. or equivalent in Materials Science or related field; 3+ years industrial experience; experience in preparing cross-section samples for failure analysis; excellent oral and written communication skills
==============================Original Headers============================== 15, 36 -- From nickm-at-wdsearch.com Wed Jul 2 19:16:16 2008 15, 36 -- Received: from viera.gnservers.com (viera.gnservers.com [72.52.242.24]) 15, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m630GGNF020326 15, 36 -- for {Microscopy-at-microscopy.net} ; Wed, 2 Jul 2008 19:16:16 -0500 15, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=wdsearch.com; 15, 36 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:X-GNServers-MailScanner-Information:X-GNServers-MailScanner-ID:X-GNServers-MailScanner:X-GNServers-MailScanner-SpamCheck:X-GNServers-MailScanner-From:X-Spam-Status:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse; 15, 36 -- b=iqF4LWowkLO2oId11bHIphljacAApPhnPBLuoC0/oSye+EOgyjnVH0KXyqN0gLTjsRoNYIWaBuuvQBw6loPq5QrRnajf+N7kxR/brb/SWWm1Thjnwj1XQoURcERuPIbm; 15, 36 -- Received: from [71.137.117.113] (helo=Nickm) 15, 36 -- by viera.gnservers.com with esmtpa (Exim 4.69) 15, 36 -- (envelope-from {nickm-at-wdsearch.com} ) 15, 36 -- id 1KECUf-0007fg-Pa 15, 36 -- for Microscopy-at-microscopy.net; Wed, 02 Jul 2008 20:16:14 -0400 15, 36 -- From: "Nick Meyler" {nickm-at-wdsearch.com} 15, 36 -- To: {Microscopy-at-microscopy.net} 15, 36 -- Subject: Clarification on Job Announcement/ Silicon Valley 15, 36 -- Date: Wed, 2 Jul 2008 17:16:10 -0700 15, 36 -- Message-ID: {007501c8dca1$ff7da990$4601a8c0-at-Nickm} 15, 36 -- MIME-Version: 1.0 15, 36 -- Content-Type: text/plain; 15, 36 -- charset="iso-8859-1" 15, 36 -- X-Mailer: Microsoft Office Outlook 11 15, 36 -- Thread-Index: AcjcbQea8FeofOPUShOJ5ddfwv6tqQANAspA 15, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 15, 36 -- X-GNServers-MailScanner-Information: Please contact the ISP for more information 15, 36 -- X-GNServers-MailScanner-ID: 1KECUf-0007fg-Pa 15, 36 -- X-GNServers-MailScanner: Not scanned: please contact your Internet E-Mail Service Provider for details 15, 36 -- X-GNServers-MailScanner-SpamCheck: 15, 36 -- X-GNServers-MailScanner-From: nickm-at-wdsearch.com 15, 36 -- X-Spam-Status: No 15, 36 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 15, 36 -- X-AntiAbuse: Primary Hostname - viera.gnservers.com 15, 36 -- X-AntiAbuse: Original Domain - microscopy.net 15, 36 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 15, 36 -- X-AntiAbuse: Sender Address Domain - wdsearch.com 15, 36 -- Content-Transfer-Encoding: 8bit 15, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m630GGNF020326 ==============================End of - Headers==============================
Are there any people (realistically speaking) who are experts on SEM and TEM but have no education beyond a BS degree? What chance is there that someone with a BS could perform the following duties?
Feedback will be very welcome, and might result in some changes to the parameters of the position.
Thank you, Nick Meyler ph (818)597-3200 ext. 211
Electron Microscopist:
My client seeks a highly skilled Electron Microscopist. In this role you will use transmission (TEM) and scanning electron microscopy (SEM) to evaluate and contribute to nanotechnology materials development and process monitoring at the company. Responsibilities include measuring a wide variety of materials and devices, preparing samples, tracking processes and communicating findings to accelerate development. In addition, you will be expected to streamline their microscopy methodologies. You will be a regular participant in group meetings and present your results and insights. As available, you will assist in other projects and develop expertise in other characterization tools.
Requirements: B.S. or equivalent in Materials Science or related field; 3+ years industrial experience; experience in preparing cross-section samples for failure analysis; excellent oral and written communication skills. Should live in California already (much preferred).
==============================Original Headers============================== 11, 36 -- From nickm-at-wdsearch.com Wed Jul 2 20:15:14 2008 11, 36 -- Received: from viera.gnservers.com (viera.gnservers.com [72.52.242.24]) 11, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m631FEK7002657 11, 36 -- for {Microscopy-at-microscopy.net} ; Wed, 2 Jul 2008 20:15:14 -0500 11, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=wdsearch.com; 11, 36 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:X-GNServers-MailScanner-Information:X-GNServers-MailScanner-ID:X-GNServers-MailScanner:X-GNServers-MailScanner-SpamCheck:X-GNServers-MailScanner-From:X-Spam-Status:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse; 11, 36 -- b=FgKcMG2Nk2GsYuLb2/OX54jTVnPReUFqp/3ZwpaJISkLMjMvax02wXtna3cNp59FN/QMbcGlQfEvyoMR8f5Qb6gmZKAm5id3BQ66hLnvoBttp7JJiqmkxcUvaOAOArpP; 11, 36 -- Received: from [71.137.117.113] (helo=Nickm) 11, 36 -- by viera.gnservers.com with esmtpa (Exim 4.69) 11, 36 -- (envelope-from {nickm-at-wdsearch.com} ) 11, 36 -- id 1KEDPg-0005UN-Nc 11, 36 -- for Microscopy-at-microscopy.net; Wed, 02 Jul 2008 21:15:08 -0400 11, 36 -- From: "Nick Meyler" {nickm-at-wdsearch.com} 11, 36 -- To: {Microscopy-at-microscopy.net} 11, 36 -- Subject: Survey Question about TEM and SEM Experts with BS Degrees 11, 36 -- Date: Wed, 2 Jul 2008 18:15:06 -0700 11, 36 -- Message-ID: {004101c8dcaa$3b5f1fe0$4601a8c0-at-Nickm} 11, 36 -- MIME-Version: 1.0 11, 36 -- Content-Type: text/plain; 11, 36 -- charset="iso-8859-1" 11, 36 -- X-Mailer: Microsoft Office Outlook 11 11, 36 -- Thread-Index: Acjcqjqxpzh25b3PT2W/+Ew/BF4yRw== 11, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 11, 36 -- X-GNServers-MailScanner-Information: Please contact the ISP for more information 11, 36 -- X-GNServers-MailScanner-ID: 1KEDPg-0005UN-Nc 11, 36 -- X-GNServers-MailScanner: Not scanned: please contact your Internet E-Mail Service Provider for details 11, 36 -- X-GNServers-MailScanner-SpamCheck: 11, 36 -- X-GNServers-MailScanner-From: nickm-at-wdsearch.com 11, 36 -- X-Spam-Status: No 11, 36 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 11, 36 -- X-AntiAbuse: Primary Hostname - viera.gnservers.com 11, 36 -- X-AntiAbuse: Original Domain - microscopy.net 11, 36 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 11, 36 -- X-AntiAbuse: Sender Address Domain - wdsearch.com 11, 36 -- Content-Transfer-Encoding: 8bit 11, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m631FEK7002657 ==============================End of - Headers==============================
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Question: We are hoping to be in a position to purchase a high pressure freezer shortly and having to choose between Leica's Empact2 and the Baltec (now Leica!) HPM100.
Both appear to freeze well, but depending who you talk to, it appears that the larger Baltec sample may be easier to work with, but Empact2 may be better for rapid transfer for co-relative applications.
Feedback from listers (particularly re HPM100 experience) would be greatly appreciated.
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I have been using acetone for the infiltration for few years, and I never had any problem with tissues. The only problem I got was when I was using gelatin to pre embed cell suspension or bacteria. After switching to bacto Agar, this was solved. I use ethanol only for dehydration, unless I have a monolayer of cells that were grown on a plastic dish for which I want use the flat embedding technique. In that case I do use ethanol all the way from dehydration to infiltration and it works also very well.
Here is a ref. I found about this problem :
Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR. Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Microsc Res Tech. 1992 Mar 1;21(1):39-50.
The Hayat books do seem to take all possible sides of every issue.
Propylene oxide is an epoxide and I've been told that rather than helping if there are traces left, it reacts without crosslinking and so it is likely to be worse if residual PO is left relative to other solvents. The high evaporation rate of PO causes cooling and condensation of moisture if used in a fume hood draft and open.
Since I work in a facility, I have to repeat methods that people request whether I see the logic to it or not, so I do use all manner of solvents. They CAN all work well. If you look at some of the classic ultrastructure papers, you will see that people have successfully done just about everything.
I always use Type 3A molecular sieve in the bottom of my bottles of any solvent and reserve these bottles for the final 2 changes - and have threatened bad things on anyone who shakes the bottles. I initially wash the molecular sieves to remove any fines. I bake each charge of molecular sieve (~5% by vol) at 250C+ in a fume hood using a hemispherical heating mantle on a Variac set to give the correct temp (depends on the mantle wattage) measured with a thermocouple. The molecular sieve is heated in an aluminum dish with a loose cover in the fume hood. After 2 hours "at temp" the sieves are placed on a porcelain support in a glass desiccator and evacuated for cooling - so it doesn't pick up moisture - and transferred to the bottle (Quorpak, with polyseal closures) as soon as cool. The solvent is added to fill the bottle and it is left at least overnight to settle and equilibrate. I pipet from well above the sieves and discard the solvent dregs, drying and reusing the sieves: http://www.bio.umass.edu/microscopy/mol_sieves.htm
As for damaging diamond knives, I am still using the same diamond knife I received (used) when I started working at our facility in 1994 and it has no new knife marks; I do a modest amount of sectioning, and have molecular sieves in all final solvents; just take care and it is not necessarily a problem.
I wish we had some solid data on the actual rate that solvents pick up moisture. I think this would be done with a Karl Fischer coulometer setup, but I don't have access to one. We all know the dogma about moisture in solvents, but it would be nice if someone could do some tests like taking one sample from a bottle of dry solvent and then pouring out some and leaving it half full and open/closed and sampling at intervals to see what happens at some typical relative humidity. Lacking hard evidence, I use the molecular sieves for all final changes of solvents in water-sensitive applications and have no moisture problems.
Dale Callaham
vladislav_speransky-at-nih.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } If someone has that reference, I'd be interested. I used to believe } that the advantage of PO is that it evaporates without a trace - } which is easy to believe for anyone who has ever handled PO. } } We are fortunate here, noone tells us what to use or not use for } dehydration and infiltration. PO gives you a piece of mind, all my } infiltration problems disappeared and never came back since I started } using PO, 9 years ago. } } With all due respect, Hyatt books (I have almost all) are really a } vast collection of recipes and anecdotes, many of which contradict } each other. I wouldn't refer to them as a bible. There are much } better sources these days, like Bozzola/Russel, Afzelius/Maunsbach... } } For epon analogs, my understanding is one should use at least } acetone, ethanol alone won't do. I have never tried going from } ethanol straight to epon but have noticed that when the aceton is not } freshly opened, I often have infiltration issues. I would use those } small bottles of glass-distilled acetone sold by EM vendors and open } a fresh bottle every time. } } A few teaching EM labs I've ben to use molecular sieves in bottles of } pure ethanol and acetone to keep them dry. Reportedly, this can cause } problems with diamond knives... } } It all depends on what you must infiltrate, of course. While you can } do just fine w/o PO with mammalian cell culture and many tissues, } skin will be more difficult. } } Best regards, } Vlad } ________________________________________________ } Vlad Speransky, Staff Scientist } Supramolecular Structure and Function Resource } National Institute of Biomedical Imaging and Bioengineering, NIH } 13 South Dr, Rm. 3N17 MSC 5766 } Bethesda, MD 20892 } 301 496-3989 } vladislav_speransky-at-nih.gov } } } } } } From: jpchandl-at-mines.edu } } Date: July 2, 2008 4:45:12 PM EDT } } To: vladislav_speransky-at-nih.gov } } Subject: [Microscopy] Propylene Oxide vs Ethanol or Acetone } } Reply-To: jpchandl-at-mines.edu } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Paula, } } } } I don't know the reference, but I recall being told that one } } advantage of } } using propylene oxide is that the molecule gets incorporated into the } } polymerized resin. This is not so for other solvents, which can } } lead to } } localized regions of poorly polymerized resin. If you are careful, } } acetone } } and ethanol should work fine. } } } } --John } } } } John Chandler } } Colorado School of Mines } } jpchandl-at-mines.edu } } 303.384.2203 (office) } } } } } } -----Original Message----- } } X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] } } Sent: Wednesday, July 02, 2008 1:58 PM } } To: jpchandl-at-mines.edu } } Subject: [Microscopy] Re: Propylene Oxide vs Ethanol or Acetone } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } Hi Paula, } } } } If you look at nice work that has been published and take the } } minimalist } } stance, then you have to accept that acetone is perfectly capable of } } yielding beautiful results when used as the dehydrating and } } infiltration } } medium for epoxy resins. I think I read in Hayat that it is less } } hygroscopic than ethanol and has some other benefits. There may be } } some } } specific cases where propylene oxide or another solvent/combination } } could be better, but I have seen enough to believe that acetone is } } probably a perfectly good solvent in almost all cases. } } } } Along these same minimalist lines, I have seen enough excellent work } } using room temperature dehydrations that I wouldn't routinely bother } } with dehydrations in the cold except for very delicate samples. Here } } there is a slightly better case for the trouble of cold dehydration, } } since most materials become stiffer at lower temps and it may protect } } from some tissue distortions. } } } } My advice is probably worth exactly what you paid, } } } } Dale Callaham } } } } vapatpxs-at-yahoo.com wrote: } } ---------------------------------------------------------------------- } } ------ } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------- } } ------ } } } Hi listers, } } } } } } The safety folks here are starting to give the evil eye to the } } } propylene } } oxide. In the past I've skipped the po as an intermediate and used } } ETOH or } } Acetone in the infiltration steps. } } } What is the consensus, or is there one? The less toxic/flammable the } } better. } } } Thanks in advance, } } } } } } Paula :-) } } } } } } Paula Sicurello } } } VA San Diego Healthcare System } } } Microscope Facility room B141 } } } 3350 La Jolla Village Dr., MC151 } } } San Diego, CA 92161 } } } 858-552-8585 x2397 } } } } } } } } ==============================Original } } Headers============================== } } 18, 21 -- From jpchandl-at-mines.edu Wed Jul 2 15:44:24 2008 } } 18, 21 -- Received: from inspire.Mines.EDU (inspire.Mines.EDU } } [138.67.130.5]) } } 18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m62KiOeR007960 } } 18, 21 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 } } 15:44:24 -0500 } } 18, 21 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) } } 18, 21 -- by inspire.Mines.EDU (8.13.1/8.13.1) with ESMTP id } } m62KiKCq004535; } } 18, 21 -- Wed, 2 Jul 2008 14:44:23 -0600 } } 18, 21 -- From: "John Chandler" {jpchandl-at-mines.edu} } } 18, 21 -- To: {dac-at-research.umass.edu} , {microscopy-at-microscopy.com} } } 18, 21 -- References: {200807021957.m62Jvi85001469-at-ns.microscopy.com} } } 18, 21 -- Subject: RE: [Microscopy] Re: Propylene Oxide vs Ethanol } } or Acetone } } 18, 21 -- Date: Wed, 2 Jul 2008 14:44:20 -0600 } } 18, 21 -- Message-ID: {002101c8dc84$6746dbf0$ad1c438a-at-mines.edu} } } 18, 21 -- MIME-Version: 1.0 } } 18, 21 -- Content-Type: text/plain; } } 18, 21 -- charset="us-ascii" } } 18, 21 -- Content-Transfer-Encoding: 7bit } } 18, 21 -- X-Mailer: Microsoft Office Outlook 11 } } 18, 21 -- In-Reply-To: {200807021957.m62Jvi85001469-at-ns.microscopy.com} } } 18, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } } 18, 21 -- Thread-Index: AcjcfeWIUj7F6RZBQ1Srr7gE00n2uwABdEpg } } ==============================End of - } } Headers============================== } } } ==============================Original Headers============================== } 12, 23 -- From vladislav_speransky-at-nih.gov Wed Jul 2 18:22:54 2008 } 12, 23 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) } 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m62NMsU2006382 } 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 18:22:54 -0500 } 12, 23 -- X-IronPortListener: NIH_Relay } 12, 23 -- X-SBRS: None } 12, 23 -- X-IronPort-AV: E=Sophos;i="4.27,739,1204520400"; } 12, 23 -- d="scan'208";a="19729504" } 12, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) } 12, 23 -- by nihrelayxway3.hub.nih.gov with ESMTP; 02 Jul 2008 19:22:54 -0400 } 12, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) } 12, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m62NMnRN029656 } 12, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 19:22:52 -0400 } 12, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) } 12, 23 -- References: {200807022045.m62KjC5w009313-at-ns.microscopy.com} } 12, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } 12, 23 -- Message-Id: {B608A7D4-80AE-4C07-8E5B-507DADB89519-at-nih.gov} } 12, 23 -- Content-Transfer-Encoding: 7bit } 12, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} } 12, 23 -- Subject: Fwd: [Microscopy] Propylene Oxide vs Ethanol or Acetone } 12, 23 -- Date: Wed, 2 Jul 2008 19:22:08 -0400 } 12, 23 -- To: Microscopy-at-microscopy.com } 12, 23 -- X-Mailer: Apple Mail (2.753.1) } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 21 -- From dac-at-research.umass.edu Wed Jul 2 23:23:03 2008 9, 21 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m634N2oo027221 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 23:23:03 -0500 9, 21 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 9, 21 -- (authenticated bits=0) 9, 21 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m634N1eQ013865 9, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 21 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 00:23:01 -0400 9, 21 -- Message-ID: {486C626F.2050005-at-research.umass.edu} 9, 21 -- Date: Thu, 03 Jul 2008 00:23:59 -0500 9, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.13) Gecko/20080313 SeaMonkey/1.1.9 9, 21 -- MIME-Version: 1.0 9, 21 -- To: Microscopy List {microscopy-at-microscopy.com} 9, 21 -- Subject: Re: [Microscopy] Fwd: Propylene Oxide vs Ethanol or Acetone - and 9, 21 -- Moisture 9, 21 -- References: {200807022326.m62NQM1F014615-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200807022326.m62NQM1F014615-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Some months ago someone (don't remember who) recomended Acetonitrile as a substitute for propylenoxide. We've tried it in my lab and it seems to work well. I'm in a prosess of comparing structures, but so far it looks very promising, and the safety officer is very pleased. The original postings sure is in the MSA archive.
Best regards Randi Olsen Dept. Electron microscopy University of Tromso N-9037 Tromso Norway
-----Original Message----- X-from: sougratr-at-mail.nih.gov [mailto:sougratr-at-mail.nih.gov] Sent: 3. juli 2008 03:59 To: Randi Olsen
Hi,
Here is my experience :
I have been using acetone for the infiltration for few years, and I never had any problem with tissues. The only problem I got was when I was using gelatin to pre embed cell suspension or bacteria. After switching to bacto Agar, this was solved. I use ethanol only for dehydration, unless I have a monolayer of cells that were grown on a plastic dish for which I want use the flat embedding technique. In that case I do use ethanol all the way from dehydration to infiltration and it works also very well.
Here is a ref. I found about this problem :
Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR. Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Microsc Res Tech. 1992 Mar 1;21(1):39-50.
Hi! I don't understand how your safety officer can be pleased to see you working with "The substance is toxic to blood, kidneys, lungs, liver, mucous membranes, gastrointestinal tract, upper respiratory tract, skin, eyes, central nervous system (CNS). The substance may be toxic to the reproductive system. Repeated or prolonged exposure to the substance can produce target organs damage. Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs." (extract of the MSDS for acetonitrile, http://www.sciencelab.com/xMSDS-Acetonitrile-9927335)
Well I already reported that I tried to use acetonitrile during dehydration and embedding with no satisfying results. One has probably to increase the dehydration times. I didn't try ethanol dehydration and acetonitrile embedding, it may be an option.
I second the remarks of Rachid concerning the use of EthOH when flat-embedding in petri dishes. However in this case it is very important to increase the incubation with epoxy alone, since traces of ethanol may disturb the polymerization. I remember I used acetone in the past, but I concentrated only on the nuclear morphology. Perhaps it has a better extraction property, which may be appreciated (if you want to contrast a given feature) or not (if you want to keep as many structures as possible). But it works. regards, Stephane
----- Original Message ---- X-from: "Randi.Olsen-at-fagmed.uit.no" {Randi.Olsen-at-fagmed.uit.no} To: nizets2-at-yahoo.com Sent: Thursday, July 3, 2008 9:05:55 AM
Hi,
Some months ago someone (don't remember who) recomended Acetonitrile as a substitute for propylenoxide. We've tried it in my lab and it seems to work well. I'm in a prosess of comparing structures, but so far it looks very promising, and the safety officer is very pleased. The original postings sure is in the MSA archive.
Best regards Randi Olsen Dept. Electron microscopy University of Tromso N-9037 Tromso Norway
-----Original Message----- X-from: sougratr-at-mail.nih.gov [mailto:sougratr-at-mail.nih.gov] Sent: 3. juli 2008 03:59 To: Randi Olsen
Hi,
Here is my experience :
I have been using acetone for the infiltration for few years, and I never had any problem with tissues. The only problem I got was when I was using gelatin to pre embed cell suspension or bacteria. After switching to bacto Agar, this was solved. I use ethanol only for dehydration, unless I have a monolayer of cells that were grown on a plastic dish for which I want use the flat embedding technique. In that case I do use ethanol all the way from dehydration to infiltration and it works also very well.
Here is a ref. I found about this problem :
Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR. Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Microsc Res Tech. 1992 Mar 1;21(1):39-50.
Hi, Thank your for your response! It might be that the fact that the acetonitrile is more commonly in use, in stock at the faculty storeroom and not exclusively in use at the EM-department (we are followed by eagles eyes because of all our specialities) is enough to make it easier to get it accepted.
We are using ethanol for dehydrating, haven't done anything with dehydration time, we get good infiltration but as I said, still in progress of evaluating results.
Best regards Randi Olsen Dept. Electron microscopy University of Tromso N-9037 Tromso Norway
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 3. juli 2008 13:12 To: Randi Olsen
Hi! I don't understand how your safety officer can be pleased to see you working with "The substance is toxic to blood, kidneys, lungs, liver, mucous membranes, gastrointestinal tract, upper respiratory tract, skin, eyes, central nervous system (CNS). The substance may be toxic to the reproductive system. Repeated or prolonged exposure to the substance can produce target organs damage. Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs." (extract of the MSDS for acetonitrile, http://www.sciencelab.com/xMSDS-Acetonitrile-9927335)
Well I already reported that I tried to use acetonitrile during dehydration and embedding with no satisfying results. One has probably to increase the dehydration times. I didn't try ethanol dehydration and acetonitrile embedding, it may be an option.
I second the remarks of Rachid concerning the use of EthOH when flat-embedding in petri dishes. However in this case it is very important to increase the incubation with epoxy alone, since traces of ethanol may disturb the polymerization. I remember I used acetone in the past, but I concentrated only on the nuclear morphology. Perhaps it has a better extraction property, which may be appreciated (if you want to contrast a given feature) or not (if you want to keep as many structures as possible). But it works. regards, Stephane
----- Original Message ---- X-from: "Randi.Olsen-at-fagmed.uit.no" {Randi.Olsen-at-fagmed.uit.no} To: nizets2-at-yahoo.com Sent: Thursday, July 3, 2008 9:05:55 AM
Hi,
Some months ago someone (don't remember who) recomended Acetonitrile as a substitute for propylenoxide. We've tried it in my lab and it seems to work well. I'm in a prosess of comparing structures, but so far it looks very promising, and the safety officer is very pleased. The original postings sure is in the MSA archive.
Best regards Randi Olsen Dept. Electron microscopy University of Tromso N-9037 Tromso Norway
-----Original Message----- X-from: sougratr-at-mail.nih.gov [mailto:sougratr-at-mail.nih.gov] Sent: 3. juli 2008 03:59 To: Randi Olsen
Hi,
Here is my experience :
I have been using acetone for the infiltration for few years, and I never had any problem with tissues. The only problem I got was when I was using gelatin to pre embed cell suspension or bacteria. After switching to bacto Agar, this was solved. I use ethanol only for dehydration, unless I have a monolayer of cells that were grown on a plastic dish for which I want use the flat embedding technique. In that case I do use ethanol all the way from dehydration to infiltration and it works also very well.
Here is a ref. I found about this problem :
Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR. Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Microsc Res Tech. 1992 Mar 1;21(1):39-50.
Nick, Realistically, yes. Although I have not been an operator for some 31 years, I have not only been servicing SEMS for those 31 years (27 as an independent - no factory backup), but I've been helping my customers with all manner of non-instrument problems, often sample prep or operating conditions, so that they can get the information that they need. I have 3 years towards a BA in zoology and have yet to take any courses in electronics, although virtually all my electronic repairs are to component level (I do, however, have a whole bookshelf of electronics texts that have been read cover to cover). Without getting on my soapbox, the letters are of somewhat limited value in determining whether or not someone can do a given job. I'll leave it there.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: nickm-at-wdsearch.com [mailto:nickm-at-wdsearch.com] Sent: Wednesday, July 02, 2008 9:18 PM To: kenconverse-at-qualityimages.biz
Important Question (Relative to Job Description):
Are there any people (realistically speaking) who are experts on SEM and TEM but have no education beyond a BS degree? What chance is there that someone with a BS could perform the following duties?
Feedback will be very welcome, and might result in some changes to the parameters of the position.
Thank you, Nick Meyler ph (818)597-3200 ext. 211
Electron Microscopist:
My client seeks a highly skilled Electron Microscopist. In this role you will use transmission (TEM) and scanning electron microscopy (SEM) to evaluate and contribute to nanotechnology materials development and process monitoring at the company. Responsibilities include measuring a wide variety of materials and devices, preparing samples, tracking processes and communicating findings to accelerate development. In addition, you will be expected to streamline their microscopy methodologies. You will be a regular participant in group meetings and present your results and insights. As available, you will assist in other projects and develop expertise in other characterization tools.
Requirements: B.S. or equivalent in Materials Science or related field; 3+ years industrial experience; experience in preparing cross-section samples for failure analysis; excellent oral and written communication skills. Should live in California already (much preferred).
==============================Original Headers============================== 11, 36 -- From nickm-at-wdsearch.com Wed Jul 2 20:15:14 2008 11, 36 -- Received: from viera.gnservers.com (viera.gnservers.com [72.52.242.24]) 11, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m631FEK7002657 11, 36 -- for {Microscopy-at-microscopy.net} ; Wed, 2 Jul 2008 20:15:14 -0500 11, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=wdsearch.com; 11, 36 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content -Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:X-GNServers-MailScanner-I nformation:X-GNServers-MailScanner-ID:X-GNServers-MailScanner:X-GNServers-Ma ilScanner-SpamCheck:X-GNServers-MailScanner-From:X-Spam-Status:X-AntiAbuse:X -AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse; 11, 36 -- b=FgKcMG2Nk2GsYuLb2/OX54jTVnPReUFqp/3ZwpaJISkLMjMvax02wXtna3cNp59FN/QMbcGlQf EvyoMR8f5Qb6gmZKAm5id3BQ66hLnvoBttp7JJiqmkxcUvaOAOArpP; 11, 36 -- Received: from [71.137.117.113] (helo=Nickm) 11, 36 -- by viera.gnservers.com with esmtpa (Exim 4.69) 11, 36 -- (envelope-from {nickm-at-wdsearch.com} ) 11, 36 -- id 1KEDPg-0005UN-Nc 11, 36 -- for Microscopy-at-microscopy.net; Wed, 02 Jul 2008 21:15:08 -0400 11, 36 -- From: "Nick Meyler" {nickm-at-wdsearch.com} 11, 36 -- To: {Microscopy-at-microscopy.net} 11, 36 -- Subject: Survey Question about TEM and SEM Experts with BS Degrees 11, 36 -- Date: Wed, 2 Jul 2008 18:15:06 -0700 11, 36 -- Message-ID: {004101c8dcaa$3b5f1fe0$4601a8c0-at-Nickm} 11, 36 -- MIME-Version: 1.0 11, 36 -- Content-Type: text/plain; 11, 36 -- charset="iso-8859-1" 11, 36 -- X-Mailer: Microsoft Office Outlook 11 11, 36 -- Thread-Index: Acjcqjqxpzh25b3PT2W/+Ew/BF4yRw== 11, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 11, 36 -- X-GNServers-MailScanner-Information: Please contact the ISP for more information 11, 36 -- X-GNServers-MailScanner-ID: 1KEDPg-0005UN-Nc 11, 36 -- X-GNServers-MailScanner: Not scanned: please contact your Internet E-Mail Service Provider for details 11, 36 -- X-GNServers-MailScanner-SpamCheck: 11, 36 -- X-GNServers-MailScanner-From: nickm-at-wdsearch.com 11, 36 -- X-Spam-Status: No 11, 36 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 11, 36 -- X-AntiAbuse: Primary Hostname - viera.gnservers.com 11, 36 -- X-AntiAbuse: Original Domain - microscopy.net 11, 36 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 11, 36 -- X-AntiAbuse: Sender Address Domain - wdsearch.com 11, 36 -- Content-Transfer-Encoding: 8bit 11, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m631FEK7002657 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 25 -- From kenconverse-at-qualityimages.biz Thu Jul 3 07:32:19 2008 25, 25 -- Received: from mta13.adelphia.net (mta13.mail.adelphia.net [68.168.78.44]) 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m63CWJ73012198 25, 25 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 07:32:19 -0500 25, 25 -- Received: from Ken ([72.227.100.25]) by mta13.adelphia.net 25, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 25, 25 -- id {20080703123025.JLTK7723.mta13.adelphia.net-at-Ken} ; 25, 25 -- Thu, 3 Jul 2008 08:30:25 -0400 25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 25 -- To: {nickm-at-wdsearch.com} , "MSA Listserver" {microscopy-at-microscopy.com} 25, 25 -- Subject: RE: [Microscopy] Survey Question about TEM and SEM Experts with BS Degrees 25, 25 -- Date: Thu, 3 Jul 2008 08:32:06 -0400 25, 25 -- Message-ID: {BFA304D1C9674CB0A894FBA8A5D67405-at-Ken} 25, 25 -- MIME-Version: 1.0 25, 25 -- Content-Type: text/plain; 25, 25 -- charset="iso-8859-1" 25, 25 -- X-Priority: 3 (Normal) 25, 25 -- X-MSMail-Priority: Normal 25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 25, 25 -- Importance: Normal 25, 25 -- Thread-Index: AcjcqpAWV7L/fOWhRaCDTmb4L6GlLgAXItgg 25, 25 -- In-Reply-To: {200807030117.m631HUKw006796-at-ns.microscopy.com} 25, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 25, 25 -- Content-Transfer-Encoding: 8bit 25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m63CWJ73012198 ==============================End of - Headers==============================
I have to say that YES there are plenty of people (especially those coming out of the two programs for electron microscopy training out of Madison Area Technical College and San Joaquin Delta College) that have excellent experience with and without a bachelors degree. Even here at Iowa State we have non-traditional students (undergrads) take the graduate level EM courses who have a great deal of expertise and proficiency in operation of SEM's and TEM's in biological and material sciences. It really matters on the experience and not always the degree.
Tracey Pepper Iowa State University
==============================Original Headers============================== 5, 21 -- From tpepper-at-iastate.edu Thu Jul 3 08:55:36 2008 5, 21 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m63Dta6J031449 5, 21 -- for {Microscopy-at-microscopy.net} ; Thu, 3 Jul 2008 08:55:36 -0500 5, 21 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 5, 21 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m63DtakU010374 5, 21 -- for {Microscopy-at-microscopy.net} ; Thu, 3 Jul 2008 08:55:36 -0500 5, 21 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 5, 21 -- id 7cb3_36f9e290_4907_11dd_8c77_00137253420a; 5, 21 -- Thu, 03 Jul 2008 08:52:02 -0500 5, 21 -- Received: from Debug (webmail-17.iastate.edu [129.186.140.37]) 5, 21 -- by despam-10.iastate.edu (8.14.2/8.12.10) with SMTP id m63DtZTd025184 5, 21 -- for {Microscopy-at-microscopy.net} ; Thu, 3 Jul 2008 08:55:35 -0500 5, 21 -- To: Microscopy-at-microscopy.net 5, 21 -- From: "Tracey Pepper" {tpepper-at-iastate.edu} 5, 21 -- Subject: Survey question 5, 21 -- Date: Thu, 3 Jul 2008 08:55:35 -0500 (CDT) 5, 21 -- X-Mailer: Endymion MailMan Professional Edition v3.0.14 ISU Version mp9.11 5, 21 -- Message-Id: {355583610841841-at-webmail.iastate.edu} 5, 21 -- X-PMX-Version: 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.7.3.134425 5, 21 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='BODY_SIZE_1000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_600_699 0, __C230066_P1_1 0, __C230066_P2 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __PHISH_SUBJ_PHRASE5 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Many years ago (1983) I had to embed cultures or retinal pigment epithelium grown in plastic dishes. PO was out of the question. So after several changes of absolute EtOH I mixed abs. EtOH with Epon substitute 2:1 then 1:2 and finally several changes of pure Epon. I also used agitation and a time in a vacuum dessicator to be sure I got all of the EtOH out. Worked like a charm. Also, it is possible to skip abs. EtOH completely and go directly to an Epon substitute mixed with 95% EtOH. Labs doing post-embedding immunostaining do this routinely.
Geoff
sougratr-at-mail.nih.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } Here is my experience : } } I have been using acetone for the infiltration for few years, and I never had any problem with tissues. } The only problem I got was when I was using gelatin to pre embed cell suspension or bacteria. After switching to bacto Agar, this was solved. } I use ethanol only for dehydration, unless I have a monolayer of cells that were grown on a plastic dish for which I want use the flat embedding technique. In that case I do use ethanol all the way from dehydration to infiltration and it works also very well. } } Here is a ref. I found about this problem : } } Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR. } Acetonitrile as a substitute for ethanol/propylene oxide in tissue } processing for transmission electron microscopy: comparison of fine } structure and lipid solubility in mouse liver, kidney, and intestine. } Microsc Res Tech. 1992 Mar 1;21(1):39-50. } } Cheers } } Rachid } } -- } Rachid SOUGRAT } Cell Biology and Metabolism Branch } NICHD, NIH } Bldg. 18T, Rm. 101 } 18 Library Drive } Bethesda, MD 20892-5430 } USA } } Phone: 301-594-3944 } Fax : 301-402-0078 } } } ==============================Original Headers============================== } 10, 26 -- From sougratr-at-mail.nih.gov Wed Jul 2 20:54:31 2008 } 10, 26 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) } 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m631sULj009291 } 10, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Jul 2008 20:54:31 -0500 } 10, 26 -- X-IronPortListener: Outbound_SMTP } 10, 26 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) } 10, 26 -- by nihxway2out.hub.nih.gov with ESMTP; 02 Jul 2008 21:54:30 -0400 } 10, 26 -- Received: from NIHCESMLBX11.nih.gov ([156.40.71.211]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); } 10, 26 -- Wed, 2 Jul 2008 21:54:30 -0400 } 10, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 10, 26 -- Content-class: urn:content-classes:message } 10, 26 -- MIME-Version: 1.0 } 10, 26 -- Content-Type: text/plain; } 10, 26 -- charset="iso-8859-1" } 10, 26 -- Subject: acetone vs PO } 10, 26 -- Date: Wed, 2 Jul 2008 21:54:29 -0400 } 10, 26 -- Message-ID: {F87C37965595794EA0006FC1632C2625B17516-at-NIHCESMLBX11.nih.gov} } 10, 26 -- X-MS-Has-Attach: } 10, 26 -- X-MS-TNEF-Correlator: } 10, 26 -- Thread-Topic: acetone vs PO } 10, 26 -- Thread-Index: Acjcr7sP9srt0YRMSC6od3pDqlnFFA== } 10, 26 -- From: "Sougrat, Rachid (NIH/NICHD) [F]" {sougratr-at-mail.nih.gov} } 10, 26 -- To: {Microscopy-at-microscopy.com} } 10, 26 -- X-OriginalArrivalTime: 03 Jul 2008 01:54:30.0880 (UTC) FILETIME=[BBD5D600:01C8DCAF] } 10, 26 -- Content-Transfer-Encoding: 8bit } 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m631sULj009291 } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 28 -- From mcauliff-at-umdnj.edu Thu Jul 3 09:14:15 2008 9, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m63EEFmr012375 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 09:14:15 -0500 9, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 9, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 2FB71A7BB6 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 10:14:15 -0400 (EDT) 9, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 9, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 5D0A4A7BA7 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 10:14:14 -0400 (EDT) 9, 28 -- Received: from ([10.32.15.167]) 9, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.155135608; 9, 28 -- Thu, 03 Jul 2008 10:13:57 -0400 9, 28 -- MIME-version: 1.0 9, 28 -- Content-transfer-encoding: 7BIT 9, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 9, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 9, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0K3F00M8VOV9U420-at-umduwc01.umdnj.edu} for 9, 28 -- microscopy-at-microscopy.com; Thu, 03 Jul 2008 10:13:57 -0400 (EDT) 9, 28 -- Message-id: {486CDED2.2070400-at-umdnj.edu} 9, 28 -- Date: Thu, 03 Jul 2008 10:14:42 -0400 9, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 28 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 9, 28 -- To: sougratr-at-mail.nih.gov, microscopy-at-microscopy.com 9, 28 -- Subject: Re: [Microscopy] acetone vs PO 9, 28 -- References: {200807030155.m631tBto010798-at-ns.microscopy.com} 9, 28 -- In-reply-to: {200807030155.m631tBto010798-at-ns.microscopy.com} ==============================End of - Headers==============================
I want to thank everyone who participated in my survey on whether the job duties (listed below) were realistic for a BS-degreed individual. For those who are curious, the results of the tally (so far) were 17 respondents who said “Yes” and 8 respondents who said “No” or “Highly unlikely”.
I also checked with a friend (a PhD) who was ACS Chemist of the Year 2003, and he indicated that he thought a BS could definitely do the work…
So, at this point, I am still looking for someone who has a BS (or possibly AA or MS) degree, but I am not really looking for a PhD (unless that changes next week, based on results).
Also, my client really, really wants someone local to the SF Bay area (Silicon Valley) OR at least California. As far as salary is concerned, I am convinced that they will have to be competitive with the market, so let’s just say that salary is “open and negotiable” – no set limits, but needs to be realistic.
Given that restatement, I hope everyone has a great 4th of July, and if you have an AA or BS degree (but not a PhD) and live in California, and have any interest in exploring an outstanding career opportunity, please send me a resume!
Thank you. ____________________________________________________________________ I am conducting a retained search on behalf of a major pre-IPO nanotechnology startup with substantial funding, a very large IP portfolio, and under 100 employees. The position is based in Northern Silicon Valley and the company is doing some exciting research in areas like printed, flexible electronics, solar cells, power supplies, gene-assay systems and nanocrystal memory. The company offers a competitive package including benefits and stock options. If you are interested or have referrals or suggestions, please send me a resume or alert me.
My client seeks a highly skilled Electron Microscopist. In this role you will use transmission (TEM) and scanning electron microscopy (SEM) to evaluate and contribute to nanotechnology materials development and process monitoring at the company. Responsibilities include measuring a wide variety of materials and devices, preparing samples, tracking processes and communicating findings to accelerate development. In addition, you will be expected to streamline their microscopy methodologies. You will be a regular participant in group meetings and present your results and insights. As available, you will assist in other projects and develop expertise in other characterization tools.
Requirements: B.S. or equivalent in Materials Science or related field; 3+ years industrial experience; experience in preparing cross-section samples for failure analysis; excellent oral and written communication skills
==============================Original Headers============================== 16, 36 -- From nickm-at-wdsearch.com Thu Jul 3 16:22:11 2008 16, 36 -- Received: from viera.gnservers.com (viera.gnservers.com [72.52.242.24]) 16, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m63LMBbI012451 16, 36 -- for {Microscopy-at-microscopy.net} ; Thu, 3 Jul 2008 16:22:11 -0500 16, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=default; d=wdsearch.com; 16, 36 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:X-MimeOLE:X-GNServers-MailScanner-Information:X-GNServers-MailScanner-ID:X-GNServers-MailScanner:X-GNServers-MailScanner-SpamCheck:X-GNServers-MailScanner-From:X-Spam-Status:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse:X-AntiAbuse; 16, 36 -- b=yB/C4qlAsifmZZJWCgCsSyN6nGB7xFoXWsmwRgtRsz9sSgKdQuXHfX3BszQ5q65gSUid8hvsIso20+q0FID0XFEWSQUcuSh0AClgAfpFltNIR5u5ud++8BVyWnBZQHZB; 16, 36 -- Received: from [71.137.117.113] (helo=Nickm) 16, 36 -- by viera.gnservers.com with esmtpa (Exim 4.69) 16, 36 -- (envelope-from {nickm-at-wdsearch.com} ) 16, 36 -- id 1KEWFg-0006Rx-St 16, 36 -- for Microscopy-at-microscopy.net; Thu, 03 Jul 2008 17:22:05 -0400 16, 36 -- From: "Nick Meyler" {nickm-at-wdsearch.com} 16, 36 -- To: {Microscopy-at-microscopy.net} 16, 36 -- Subject: Reiteration on Silicon Valley Job Announcement for SEM/TEM Microscopist with BS degree 16, 36 -- Date: Thu, 3 Jul 2008 14:22:03 -0700 16, 36 -- Message-ID: {001101c8dd52$d6cfab90$4601a8c0-at-Nickm} 16, 36 -- MIME-Version: 1.0 16, 36 -- Content-Type: text/plain; 16, 36 -- charset="iso-8859-1" 16, 36 -- X-Mailer: Microsoft Office Outlook 11 16, 36 -- Thread-Index: AcjdUtYHrbO9R4ooTmijwRGxOXdTSQ== 16, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 36 -- X-GNServers-MailScanner-Information: Please contact the ISP for more information 16, 36 -- X-GNServers-MailScanner-ID: 1KEWFg-0006Rx-St 16, 36 -- X-GNServers-MailScanner: Not scanned: please contact your Internet E-Mail Service Provider for details 16, 36 -- X-GNServers-MailScanner-SpamCheck: 16, 36 -- X-GNServers-MailScanner-From: nickm-at-wdsearch.com 16, 36 -- X-Spam-Status: No 16, 36 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 16, 36 -- X-AntiAbuse: Primary Hostname - viera.gnservers.com 16, 36 -- X-AntiAbuse: Original Domain - microscopy.net 16, 36 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 16, 36 -- X-AntiAbuse: Sender Address Domain - wdsearch.com 16, 36 -- Content-Transfer-Encoding: 8bit 16, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m63LMBbI012451 ==============================End of - Headers==============================
We have a Zeiss Photomicroscope III and would like to buy a digital camera for it. What are people's opinions? Would something like a regular Canon or Nikon DSLR work?
Let me know what works in you lab and what type of images are you capturing mostly. I think ours will be used for paraffin work (H&E, etc) and immunofluorescence.
The microscope is beautiful with wonderful optics but the camera it has on it is so old we can't update the drivers to match the computers any more.
Thanks in advance.
Paula Sicurello VA San Diego Healthcare System Microscope Facility room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 9, 24 -- From vapatpxs-at-yahoo.com Thu Jul 3 16:36:56 2008 9, 24 -- Received: from n59.bullet.mail.sp1.yahoo.com (n59.bullet.mail.sp1.yahoo.com [98.136.44.43]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m63Latul025651 9, 24 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 16:36:55 -0500 9, 24 -- Received: from [216.252.122.217] by n59.bullet.mail.sp1.yahoo.com with NNFMP; 03 Jul 2008 21:36:55 -0000 9, 24 -- Received: from [69.147.84.114] by t2.bullet.sp1.yahoo.com with NNFMP; 03 Jul 2008 21:36:55 -0000 9, 24 -- Received: from [127.0.0.1] by omp203.mail.sp1.yahoo.com with NNFMP; 03 Jul 2008 21:36:55 -0000 9, 24 -- X-Yahoo-Newman-Property: ymail-3 9, 24 -- X-Yahoo-Newman-Id: 340926.14865.bm-at-omp203.mail.sp1.yahoo.com 9, 24 -- Received: (qmail 40820 invoked by uid 60001); 3 Jul 2008 21:36:55 -0000 9, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 24 -- s=s1024; d=yahoo.com; 9, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 9, 24 -- b=Op0AAg2DB8rOj1217SmnMcFQZ1XtxSm2FGeornIpBrhJw3szrOnEjc1sQ7Dj6/0fEusQiP2bNdq5/g3I72nGYpjD32SE0pe5Ve5SxmlUuju3hS6eVXV9ZXVUt6NTCyagRTzLGyCCDjiFgU7qgCEfmz2fzEtLis0ValdV0asqLoA=; 9, 24 -- Received: from [132.239.85.200] by web46113.mail.sp1.yahoo.com via HTTP; Thu, 03 Jul 2008 14:36:55 PDT 9, 24 -- X-Mailer: YahooMailWebService/0.7.199 9, 24 -- Date: Thu, 3 Jul 2008 14:36:55 -0700 (PDT) 9, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 9, 24 -- Reply-To: vapatpxs-at-yahoo.com 9, 24 -- Subject: Digital Camera 9, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 9, 24 -- MIME-Version: 1.0 9, 24 -- Content-Type: text/plain; charset=us-ascii 9, 24 -- Message-ID: {162024.40055.qm-at-web46113.mail.sp1.yahoo.com} ==============================End of - Headers==============================
} I don't understand how your safety officer can be pleased to see } you working with } "The substance is toxic to blood, kidneys, lungs, liver, mucous } membranes, gastrointestinal tract, upper respiratory } tract, skin, eyes, central nervous system (CNS). The substance may } be toxic to the reproductive system. } Repeated or prolonged exposure to the substance can produce target } organs damage. Repeated exposure to a } highly toxic material may produce general deterioration of health } by an accumulation in one or many human } organs." (extract of the MSDS for acetonitrile, http:// } www.sciencelab.com/xMSDS-Acetonitrile-9927335) } Simple - we don't tell them. Most of what is used for EM preparation is bad for your health. OsO4 is at least as bad as PO. Actual exposure to both can be easily avoided if you know what you are doing. Both I would keep away from young people, those who think "this won't happen to me" (like I did).
} I remember I used acetone in the past, but I concentrated only on } the nuclear morphology. Perhaps it has a better extraction } property, which may be appreciated (if you want to contrast a given } feature) or not (if you want to keep as many structures as possible).
Actually, there was a paper from T.J.Beveridge lab, back in late 80s, I think, where they compared acetone to ethanol as a dehydration agent by analyzing the exchanged fluids to find out how much stuff gets washed out. There was less extraction with acetone. Still, I almost never dehydrate in acetone, because ethanol is so much nicer to handle.
Many thanks to Dale and others for sharing their experience!..
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 6, 23 -- From vladislav_speransky-at-nih.gov Thu Jul 3 16:47:14 2008 6, 23 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m63LlEWI006099 6, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 16:47:14 -0500 6, 23 -- X-IronPortListener: NIH_Relay 6, 23 -- X-SBRS: None 6, 23 -- X-IronPort-AV: E=Sophos;i="4.27,744,1204520400"; 6, 23 -- d="scan'208";a="200800121" 6, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 6, 23 -- by nihrelayxway4.hub.nih.gov with ESMTP; 03 Jul 2008 17:46:52 -0400 6, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 6, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m63Lkpfp002794 6, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 17:46:51 -0400 6, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 23 -- References: {200807031104.m63B4i7s016739-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 23 -- Message-Id: {ED1D5991-4191-40C6-BE61-030945E5F317-at-nih.gov} 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 6, 23 -- Subject: Fwd: [Microscopy] acetone vs PO 6, 23 -- Date: Thu, 3 Jul 2008 17:46:09 -0400 6, 23 -- To: Microscopy-at-microscopy.com 6, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
The aim is to perfuse the organ in question at its systolic arterial pressure. Assuming you are transcardially perfusing in a mammal the rule of thumb is to deliver fluid at 100 mm Hg. As mercury is 13.6x denser than water, you need a head of 1.36 meters of aqueous perfusion fluid or about 4½ feet.
You also need to consider flow rate too. I recommend using large bore (but flexible) tubing from the bag/bottle in question and the largest diameter perfusion cannula (blunt) cannula as will fit in the ascending aorta.
Assuming you are perfusing the eye (same for brain) it will speed matters to clamp off the descending vessels at the level of the diaphragm (just above the liver) so all your flow goes to the upper half of the body.
Maybe such a histological question would get a better response from Histonet? They're very helpful people too. histonet-at-lists.utsouthwestern.edu
good luck! -David
David A. Wright, PhD University of Chicago Section of Neurosurgery
---- Original message ---- } Date: Thu, 3 Jul 2008 00:54:38 -0500 } From: dianavd-at-eye.usyd.edu.au } Subject: [Microscopy] perfusion fixation } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } ---------------------------------------------------------------------------- It's years since I've had to perfuse for EM and I can't remember the height above the animal to hang the bottle. Can anyone help?
Thanks
Diana } } Diana van Driel } } Discipline of Ophthalmology } Sydney University
==============================Original Headers============================== 12, 22 -- From dw18-at-uchicago.edu Fri Jul 4 11:02:30 2008 12, 22 -- Received: from smtp01.uchicago.edu (smtp01.uchicago.edu [128.135.12.77]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m64G2UKN024560 12, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Jul 2008 11:02:30 -0500 12, 22 -- Received: from m4500-03.uchicago.edu (m4500-03.uchicago.edu [128.135.249.218]) 12, 22 -- by smtp01.uchicago.edu (8.13.8/8.13.8) with ESMTP id m64G2GFB012560; 12, 22 -- Fri, 4 Jul 2008 11:02:17 -0500 12, 22 -- Received: (from m4500-03.uchicago.edu [128.135.249.212]) 12, 22 -- by m4500-03.uchicago.edu (MOS 3.8.5-GA) 12, 22 -- with HTTP/1.1 id BBX49399 (AUTH dw18-at-uchicago.edu); 12, 22 -- Fri, 4 Jul 2008 11:02:13 -0500 (CDT) 12, 22 -- From: "David A. Wright" {dw18-at-uchicago.edu} 12, 22 -- Subject: Re: [Microscopy] perfusion fixation 12, 22 -- To: Microscopy-at-microscopy.com 12, 22 -- Cc: dianavd-at-eye.usyd.edu.au 12, 22 -- X-Mailer: Mirapoint Webmail Direct 3.8.5-GA 12, 22 -- MIME-Version: 1.0 12, 22 -- Content-Type: text/plain; charset=iso-8859-1 12, 22 -- Message-Id: {20080704110213.BBX49399-at-m4500-03.uchicago.edu} 12, 22 -- Date: Fri, 4 Jul 2008 11:02:13 -0500 (CDT) 12, 22 -- Content-Transfer-Encoding: 8bit 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m64G2UKN024560 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Osmium precipitates after silver enhancement
Question: I have been optimizing immunostaining procedure for EM using nanogold and HQ silver in rat tissue perfused with 4% PFA and 0.5 % glutaraldehyde. It turned out that after osmication (down to 0.25%, 30min), we get fine precipitates (~5~10 nm) that interferes with our staining. We tried to lower osmium concentrations to 0.1%, which removed precipitates but very poor contrast which we cannot use. I was wondering if you have experienced anything like this and happen to know good ways to avoid this.
I'm trying to stabilize some very delicate polymers for low-dose TEM imaging(100-1000e/A). These polymers cannot be rewet, so I can't put them in solution and plunge freeze. I'd like to deposit a stabilization layer such as carbon, but I don't want to damage the polymers by depositing 4000k atoms all over them (heat/velocity). I've heard that using indirect arc-evaporation and simply increasing the pressure in the chamber forms super low dense film morphology, which wouldn't be very stabilizing. We’re thinking maybe PLD is the most controllable. Does anyone know of a way to deposit a tight encasing layer gently?
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our lab has recently purchased a FEG-SEM JEOL 7001F and we are finding difficult to make sure the alignment is optimized.
The problems are as follows:
The aperture mechanical alignment is to be performed the by JEOL technicians and the users are left with the electronic alignment. However, after electronic alignment it is hardly ever possible to prevent the image form shifting while over and underfocusing. In the old days this meant that the aperture alignment was not optimizedÖ Should then the users carry out the mechanical alignment as well, and for all the voltage/current conditions? Or is the electronic compensation enough and we should stick to it?
Another problem is that when the user performs the electronic alignment of the aperture, the central object chosen moves swiftly out of the screen and the user has to shift the sample in order to follow it or has to choose another central object. This happens at all magnifications. Is it possible that a correction for this problem can be introduced in the electronics?
Thanking in advance,
PatrÌcia Carvalho Instituto Superior TÈcnico Technical University of Lisbon
Have you looked into plasma-enhanced chemical vapor deposition? There are several configurations for generating the reactive vapor, some of which may be more gentle than others. One method that admits naphthalene vapor through a column with inductively coupled plasma generation should keep the plasma away from your sample. In some experiments, I reversed the polarity of a DC sputter coater and while I got nice films with naphthlene on mica, the plasma at the surface of polymer films was too much and they broke; maybe the remote generation method would work more gently. It looks a little less complicated and expensive than the PLD method.
Dale
j.whitt31-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm trying to stabilize some very delicate polymers for low-dose TEM } imaging(100-1000e/A). These polymers cannot be rewet, so I can't put them } in solution and plunge freeze. I'd like to deposit a stabilization layer } such as carbon, but I don't want to damage the polymers by depositing 4000k } atoms all over them (heat/velocity). I've heard that using indirect } arc-evaporation and simply increasing the pressure in the chamber forms } super low dense film morphology, which wouldn't be very stabilizing. We’re } thinking maybe PLD is the most controllable. Does anyone know of a way to } deposit a tight encasing layer gently? } } Thanks! } } Stanly } } } } } } ==============================Original Headers============================== } 8, 45 -- From j.whitt31-at-gmail.com Sun Jul 6 19:47:50 2008 } 8, 45 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.176]) } 8, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m670lmL8024497 } 8, 45 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Jul 2008 19:47:49 -0500 } 8, 45 -- Received: by wa-out-1112.google.com with SMTP id j5so1443150wah.4 } 8, 45 -- for {Microscopy-at-microscopy.com} ; Sun, 06 Jul 2008 17:47:47 -0700 (PDT) } 8, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 45 -- d=gmail.com; s=gamma; } 8, 45 -- h=domainkey-signature:received:received:from:to:subject:date } 8, 45 -- :mime-version:content-type:content-transfer-encoding:x-mailer } 8, 45 -- :thread-index:content-language:x-cr-hashedpuzzle:x-cr-puzzleid } 8, 45 -- :message-id; } 8, 45 -- bh=7Ihp95VRWbPXDBwOx4jAFOjp8LiPH7y0dV5ApaTjHXg=; } 8, 45 -- b=s/8cxzNQwpJ6+iO2spaDonbkBahAG1Fmv8MG47FgBxuakVmnxJK1aqyQWFeQUIUdEH } 8, 45 -- EpuQY0v0PBbaGW0nyX3MIhkLP1mrOPOeBy2u0rLEDNiqhxfjRFUNPzOdcmVVor9nVo6g } 8, 45 -- Evf2OGrR0mU9hAd372YfQMOe3AOwbC87XbvI0= } 8, 45 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 8, 45 -- d=gmail.com; s=gamma; } 8, 45 -- h=from:to:subject:date:mime-version:content-type } 8, 45 -- :content-transfer-encoding:x-mailer:thread-index:content-language } 8, 45 -- :x-cr-hashedpuzzle:x-cr-puzzleid:message-id; } 8, 45 -- b=sLtANOjyeg+Pf99kEuf3ch3cSwIr/YlGIiPDTy4bLy9jvDLFj75bL+VbiCMvhzsGao } 8, 45 -- pohahPFnnfIVggPgmCnqOo59jrEyiuratBAjqg90B8W8gTI59O3pspjT+1m7UeezS2qY } 8, 45 -- QbTs44P+DLxkT1SovVQTwiT30CR+rbN0Zhb28= } 8, 45 -- Received: by 10.115.108.1 with SMTP id k1mr5738509wam.78.1215391667042; } 8, 45 -- Sun, 06 Jul 2008 17:47:47 -0700 (PDT) } 8, 45 -- Received: from hyperion ( [68.124.176.28]) } 8, 45 -- by mx.google.com with ESMTPS id m28sm5876142poh.10.2008.07.06.17.47.45 } 8, 45 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); } 8, 45 -- Sun, 06 Jul 2008 17:47:45 -0700 (PDT) } 8, 45 -- From: "Stanly Kemish" {j.whitt31-at-gmail.com} } 8, 45 -- To: {Microscopy-at-microscopy.com} } 8, 45 -- Subject: TEM stabilization layer for delicate polymers } 8, 45 -- Date: Sun, 6 Jul 2008 17:47:40 -0700 } 8, 45 -- MIME-Version: 1.0 } 8, 45 -- Content-Type: text/plain; } 8, 45 -- charset="iso-8859-1" } 8, 45 -- X-Mailer: Microsoft Office Outlook 12.0 } 8, 45 -- Thread-Index: Acjfyp9bnXmorwawTLWuuw8iaPGGQwAAD+4g } 8, 45 -- Content-Language: en-us } 8, 45 -- x-cr-hashedpuzzle: AEOF AhB0 Ao4t BfkG CsLk DAp2 Dm8f F+6R GR8k Gjqy GnhE Gn5U HiQ/ H0mq I71k KLB6;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{F212C06A-58B7-4BAF-B0A0-36F06D4A672B};agAuAHcAaABpAHQAdAAzADEAQABnAG0AYQBpAGwALgBjAG8AbQA=;Mon, 07 Jul 2008 00:47:35 GMT;VABFAE0AIABzAHQAYQBiAGkAbABpAHoAYQB0AGkAbwBuACAAbABhAHkAZQByACAAZgBvAHIAIABkAGUAbABpAGMAYQB0AGUAIABwAG8AbAB5AG0AZQByAHMA } 8, 45 -- x-cr-puzzleid: {F212C06A-58B7-4BAF-B0A0-36F06D4A672B} } 8, 45 -- Message-ID: {487167b1.1cef600a.276d.4066-at-mx.google.com} } 8, 45 -- Content-Transfer-Encoding: 8bit } 8, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m670lmL8024497 } ==============================End of - Headers==============================
Hello All, } } I am a geologist and I am looking at purchasing a trinocular = } polarizing petrographic microscope for looking at thin sections with = } both transmitted and reflected light capabilities. At the same time, I = } am on a tight budget. Any recommedations would be appreciated. } } Thanks, } } Jim. }
==============================Original Headers============================== 2, 22 -- From renaudgeological-at-execulink.com Mon Jul 7 14:15:45 2008 2, 22 -- Received: from smtp2.execulink.net (smtp2.execulink.net [69.63.44.83]) 2, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m67JFj7Z005196 2, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jul 2008 14:15:45 -0500 2, 22 -- Received: from RGC (ppp32.ac3.56k.execulink.com [209.239.15.32]) 2, 22 -- by smtp2.execulink.net (8.13.1/8.13.1) with SMTP id m67JGr27022078 2, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jul 2008 15:17:01 -0400 2, 22 -- Message-ID: {00bc01c8e065$d9bc3c90$390fefd1-at-RGC} 2, 22 -- From: "Renaud Geological Consulting Ltd." {renaudgeological-at-execulink.com} 2, 22 -- To: {Microscopy-at-microscopy.com} 2, 22 -- Subject: [Microscopy] Petrographic Microscopes 2, 22 -- Date: Mon, 7 Jul 2008 15:15:24 -0400 2, 22 -- MIME-Version: 1.0 2, 22 -- Content-Type: text/plain; 2, 22 -- format=flowed; 2, 22 -- charset="iso-8859-1"; 2, 22 -- reply-type=original 2, 22 -- Content-Transfer-Encoding: 7bit 2, 22 -- X-Priority: 3 2, 22 -- X-MSMail-Priority: Normal 2, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 2, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
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Email: bplowman-at-pacific.edu Name: Barbara Plowman
Organization: Univ of the Pacific Arthur A. Dugoni School of Dentistry
Title-Subject: [Filtered] digital camera
Question: I would like to know if high quality images can be obtained from a regular digital camera on a SEM. It has been suggested to me but, I am skeptical. Any advice would be appreciated. Barbara Plowman Univ of the Pacific Arthur A. Dugoni School of Denistry 2155 Webster Room 400 San Francisco, CA 94598 email: bplowman-at-pacific.edu
After receiving many replies the consensus seems to be----propylene oxide is not necessary. Some people still keep it around if they have tissue that is difficult to infiltrate.
Several people dehydrate in ETOH and then transition into Acetone and use Acetone as the intermediate solvent for infiltration.
Other people just use either Ethanol or Acetone for the entire dehydration and infiltration.
So if you, like me have safety people giving the evil eye to your PO and you don't want to work with an additional toxic, nasty chemical, you don't have to use PO.
We work with enough things that are carcinogenic, mutagenic, teratogenic or will kill you outright. It's nice to be able to eliminate at least one of the icky chemicals.
I just thought I'd let you know. Also, thanks to all who replied.
Paula :-)
Paula Sicurello VA San Diego Healthcare System Microscope Facility room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 12, 24 -- From vapatpxs-at-yahoo.com Tue Jul 8 11:20:05 2008 12, 24 -- Received: from n63.bullet.mail.sp1.yahoo.com (n63.bullet.mail.sp1.yahoo.com [98.136.44.33]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m68GK5d4004479 12, 24 -- for {microscopy-at-microscopy.com} ; Tue, 8 Jul 2008 11:20:05 -0500 12, 24 -- Received: from [216.252.122.216] by n63.bullet.mail.sp1.yahoo.com with NNFMP; 08 Jul 2008 16:20:04 -0000 12, 24 -- Received: from [69.147.65.169] by t1.bullet.sp1.yahoo.com with NNFMP; 08 Jul 2008 16:20:04 -0000 12, 24 -- Received: from [127.0.0.1] by omp504.mail.sp1.yahoo.com with NNFMP; 08 Jul 2008 16:20:04 -0000 12, 24 -- X-Yahoo-Newman-Property: ymail-3 12, 24 -- X-Yahoo-Newman-Id: 843885.41428.bm-at-omp504.mail.sp1.yahoo.com 12, 24 -- Received: (qmail 12599 invoked by uid 60001); 8 Jul 2008 16:20:02 -0000 12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 24 -- s=s1024; d=yahoo.com; 12, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 12, 24 -- b=b+5iNDzxqN2pAmQ1PjKOELvvs5/FUUmFEDfNSYWt/vgBq3QK5D1uQgcKnnSMWSS6R2l65V0VcbcnzNp5V3ZY3dz6s9gB/rNLv9rnTJiu972+bo26hl64ax5rGLMlhmz4eU2cI6zKb09+sW5nHaKKOOsuef0Vc/VILBBYw5Oldzk=; 12, 24 -- Received: from [132.239.85.200] by web46109.mail.sp1.yahoo.com via HTTP; Tue, 08 Jul 2008 09:20:02 PDT 12, 24 -- X-Mailer: YahooMailWebService/0.7.199 12, 24 -- Date: Tue, 8 Jul 2008 09:20:02 -0700 (PDT) 12, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 12, 24 -- Reply-To: vapatpxs-at-yahoo.com 12, 24 -- Subject: Propylene Oxide vs Ethanol or Acetone results 12, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 12, 24 -- MIME-Version: 1.0 12, 24 -- Content-Type: text/plain; charset=us-ascii 12, 24 -- Message-ID: {386574.10983.qm-at-web46109.mail.sp1.yahoo.com} ==============================End of - Headers==============================
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Email: jwdeng-at-imr.ac.cn Name: JW Deng
Title-Subject: [Filtered] How to esmate the size of an objective aperture's size in reciprocal space
Question: Dear All,
As mentioned in many TEM books, we can measured an TEM objective aperture's size in reciprocal space in diffraction mode. We can also know the physical size of the aperture. But what's the relationship between these two size. Is it just a simple linear relationship or is it more complex? Are there any exsit formula. So I can use it to estimate the phycial size of an aperture which I have to fabricate if I want obtain an special size in recipro space.
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Email: bill.mcmanus-at-usu.edu Name: Bill McManus
Organization: Utah State University
Title-Subject: [Filtered] Lab usage by outside businesses
Question: I had heard that it is illegal for a University or any public entity to offer services to the general public and private businesses, as it is unfair competition, and equipment may be funded by state and/or federal funds, which stipulate that only the prescribed uses of the funding may be undertaken. But, I see links to websites for just such work at amazingly low prices. To hire a knowlegable EM TECH for $35/ hour is not possible for a private industry to match. Rental of a TEM for only $70/ hour is also only possible if the instrument was purchased with public funds and there is no financing to be repay. The typical equipment loan is for 15 years, add maintenance, and it becomes quickly apparent that $70.00/ hour is an unrealistic price, and not even close to profitable. What is the current status? Can federal purchased equipment be used by outside private individuals? Is it the policy of public Universities to ignor such laws, if they exist? Or are University technical labs simple unaware of the laws? I realize this has probably been discussed in the past, but I think that I may be advisable to revisit this issue
Hi Bill, It is not illegal to offer public services (though a particular organization may have rules against such activities). The only stipulation (which I often see violated by some university labs) is that if you are a tax supported institution, you must charge at least "prevailing market rates". Generally this means making a survey every year and adjusting one's rates accordingly. Of course one can charge more than market rates. No law against that. john
At 07:56 PM 7/8/2008, bill.mcmanus-at-usu.edu wrote: } Organization: Utah State University } } Title-Subject: [Filtered] Lab usage by outside businesses } } Question: I had heard that it is illegal for a University or any } public entity to offer services to the general public and private } businesses, as it is unfair competition, and equipment may be funded } by state and/or federal funds, which stipulate that only the } prescribed uses of the funding may be undertaken. But, I see links } to websites for just such work at amazingly low prices. To hire a } knowlegable EM TECH for $35/ hour is not possible for a private } industry to match. Rental of a TEM for only $70/ hour is also only } possible if the instrument was purchased with public funds and there } is no financing to be repay. The typical equipment loan is for 15 } years, add maintenance, and it becomes quickly apparent that $70.00/ } hour is an unrealistic price, and not even close to profitable. What } is the current status? Can federal purchased equipment be used by } outside private individuals? Is it the policy of public Universities } to ignor such laws, if they exist? Or are University technical labs } simple unaware of the laws? I realize this has probably been } discussed in the past, but I think that I may be advisable to revisit } this issue
==============================Original Headers============================== 3, 23 -- From donovan-at-uoregon.edu Wed Jul 9 00:40:09 2008 3, 23 -- Received: from QMTA03.westchester.pa.mail.comcast.net (qmta03.westchester.pa.mail.comcast.net [76.96.62.32]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m695e8aM002558 3, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 00:40:09 -0500 3, 23 -- Message-Id: {200807090540.m695e8aM002558-at-ns.microscopy.com} 3, 23 -- Received: from OMTA03.westchester.pa.mail.comcast.net ([76.96.62.27]) 3, 23 -- by QMTA03.westchester.pa.mail.comcast.net with comcast 3, 23 -- id nToh1Z0010bG4ec530sA00; Wed, 09 Jul 2008 05:40:08 +0000 3, 23 -- Received: from SOURCE.uoregon.edu ([71.193.189.123]) 3, 23 -- by OMTA03.westchester.pa.mail.comcast.net with comcast 3, 23 -- id nhg71Z00C2gBFvr3Phg8Nh; Wed, 09 Jul 2008 05:40:08 +0000 3, 23 -- X-Authority-Analysis: v=1.0 c=1 a=dw17-r_YoFEA:10 a=iNdFky6oHIQA:10 3, 23 -- a=YyyMJ8p-baBeSaBaPkgA:9 a=0i_81L6iCEK5pgKpiSoA:7 3, 23 -- a=5CymB-6ucSoaAQqhqnSafCxN7kkA:4 a=50e4U0PicR4A:10 3, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 3, 23 -- Date: Tue, 08 Jul 2008 22:40:08 -0700 3, 23 -- To: microscopy-at-microscopy.com, bill.mcmanus-at-usu.edu 3, 23 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 3, 23 -- Subject: Re: [Microscopy] viaWWW: Lab usage by outside businesses 3, 23 -- In-Reply-To: {200807090256.m692uOlo020674-at-ns.microscopy.com} 3, 23 -- References: {200807090256.m692uOlo020674-at-ns.microscopy.com} 3, 23 -- Mime-Version: 1.0 3, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
The subtended angle (radians) of the objective aperture is the diameter (radius for semiangle) divided by the focal length of the lens. Actually it is the tangent of the angle, but we're talking about small angles here where (approximately) tan (a) = sin (a) = a.
You can calculate the focal length of the lens with a known aperture and a standard diffraction pattern. Just use Braggs law to calculate the diffraction angles for your electron energy. Wavelengths for different energies should be most texts. Once you know the focal length, you can easily calculate the aperture size for a given angle.
Off the top of my head... 100kV 0.037A 120kV 0.0335A 200kV 0.0251A 300kV 0.0197A
Cheers, Henk
At 10:51 PM 07/08/08, jwdeng-at-imr.ac.cn wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 12, 27 -- From colijn.1-at-osu.edu Wed Jul 9 07:12:14 2008 12, 27 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m69CCEFe030135 12, 27 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 07:12:14 -0500 12, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 12, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31224) 12, 27 -- id {01MWY0Z4M9WW8XY1UG-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 12, 27 -- Wed, 09 Jul 2008 08:12:13 -0400 (EDT) 12, 27 -- Received: from HOC1.ecr6.ohio-state.edu 12, 27 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 12, 27 -- (PMDF V6.3-x18 #31224) 12, 27 -- with ESMTPA id {01MWY0Z482HY8Y2HD8-at-ecr6.ohio-state.edu} ; Wed, 12, 27 -- 09 Jul 2008 08:12:13 -0400 (EDT) 12, 27 -- Date: Wed, 09 Jul 2008 08:11:17 -0400 12, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 12, 27 -- Subject: Re: [Microscopy] viaWWW: How to esmate the size of an objective 12, 27 -- aperture's size in 12, 27 -- In-reply-to: {200807090251.m692pXWi009793-at-ns.microscopy.com} 12, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 12, 27 -- To: jwdeng-at-imr.ac.cn 12, 27 -- Cc: microscopy-at-microscopy.com 12, 27 -- Message-id: {7.0.1.0.2.20080709080134.03401310-at-osu.edu} 12, 27 -- MIME-version: 1.0 12, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 12, 27 -- Content-type: text/plain; charset=us-ascii; format=flowed 12, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 12, 27 -- References: {200807090251.m692pXWi009793-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: U.J.Potter-at-bath.ac.uk Name: Ursula Potter
Organization: University of Bath UK
Title-Subject: [Filtered] Black precipitate in sperm TEM sections
Question: Dear All,
I have been preparing Drosophila sperm in and out of seminal vesicles and found that a black precipitate is present in the tissue. It is either fine grain particles or very dense particles up to about 60nm in size. Resin only areas of a section are clean.
The tissue was prepared using Glutaraldhyde in sodium cacodylate, osmium, encapsulation of pellet in agarose, uranyl acetate block stain, acetone dehydration & embedding in Spurr resin. I used no section staining. The structure of the sperm is great - the precipitate ruins everything! Other tissues I've prepared recently are clear of precipitate.
I would be very grateful for any advice/comments on how to resolve the problem!
I suspect that there is incomplete dehydration. I have done similar work on testis embedded in Epon and had exceptional micrographs but used times for dehydration that were a bit longer than for other drosophila tissues.
Is your acetone a dry one (water 0.1%) like Mallinckrodt 2440? From a bottle that has not been open long? Have you looked at sperm as soon as the beam hit them? If so, can you see the precipitation forming as the spot heats up? This happened when I had trouble with bacteria inside cells getting the precipitate just over them and the centriols(!). It was solved by increasing the dehydration time slightly and by an additional 100% step from a bottle that had not been opened more than a few times.
You do not mention that you used tannic acid so there should be no problem there.
Precipitation does not bring back good memories! Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form. } Remember this posting is most likely not from a Subscriber, so when replying } please copy both U.J.Potter-at-bath.ac.uk as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } Email: U.J.Potter-at-bath.ac.uk } Name: Ursula Potter } Organization: University of Bath UK } Title-Subject: [Filtered] Black precipitate in sperm TEM sections } } I have been preparing Drosophila sperm in and out of seminal vesicles } and found that a black precipitate is present in the tissue. It is } either fine grain particles or very dense particles up to about 60nm } in size. Resin only areas of a section are clean. } } The tissue was prepared using Glutaraldhyde in sodium cacodylate, } osmium, encapsulation of pellet in agarose, uranyl acetate block } stain, acetone dehydration & embedding in Spurr resin. I used no } section staining. The structure of the sperm is great - the } precipitate ruins everything! Other tissues I've prepared recently } are clear of precipitate. } } I would be very grateful for any advice/comments on how to resolve the } problem! } } Thanks } Ursula
==============================Original Headers============================== 10, 27 -- From connellyps-at-nhlbi.nih.gov Wed Jul 9 10:08:51 2008 10, 27 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m69F8ptl030136 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 10:08:51 -0500 10, 27 -- X-IronPortListener: Outbound_SMTP 10, 27 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) 10, 27 -- by nihxway2out.hub.nih.gov with ESMTP; 09 Jul 2008 11:08:50 -0400 10, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); 10, 27 -- Wed, 9 Jul 2008 11:08:46 -0400 10, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.165]) with Microsoft Exchange Server HTTP-DAV ; 10, 27 -- Wed, 9 Jul 2008 15:08:45 +0000 10, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 27 -- Date: Wed, 09 Jul 2008 11:08:43 -0400 10, 27 -- Subject: Re: [Microscopy] viaWWW: Black precipitate in sperm TEM sections 10, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 27 -- To: {U.J.Potter-at-bath.ac.uk} 10, 27 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 27 -- Message-ID: {C49A4CBB.2045%connellyps-at-nhlbi.nih.gov} 10, 27 -- Thread-Topic: [Microscopy] viaWWW: Black precipitate in sperm TEM sections 10, 27 -- Thread-Index: Acjh1a0w67xvFk3IEd2oigANk2Yv1A== 10, 27 -- In-Reply-To: {200807091311.m69DB5bb020332-at-ns.microscopy.com} 10, 27 -- Mime-version: 1.0 10, 27 -- Content-type: text/plain; 10, 27 -- charset="ISO-8859-1" 10, 27 -- X-OriginalArrivalTime: 09 Jul 2008 15:08:46.0574 (UTC) FILETIME=[AF5228E0:01C8E1D5] 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m69F8ptl030136 ==============================End of - Headers==============================
On Jul 6, 2008, at 5:48 PM, j.whitt31-at-gmail.com wrote:
} I'm trying to stabilize some very delicate polymers for low-dose TEM } imaging(100-1000e/A). These polymers cannot be rewet, so I can't } put them } in solution and plunge freeze. I'd like to deposit a stabilization } layer } such as carbon, but I don't want to damage the polymers by } depositing 4000k } atoms all over them (heat/velocity). I've heard that using indirect } arc-evaporation and simply increasing the pressure in the chamber } forms } super low dense film morphology, which wouldn't be very } stabilizing. We’re } thinking maybe PLD is the most controllable. Does anyone know of a } way to } deposit a tight encasing layer gently?
Dear Stanly, I have two ideas for you: You can cool the specimen to LN2 temp without plunge freezing--just put it in a cryostage, put the stage into the EM, then cool the stage. Most of the heat transmitted to the specimen from a C arc comes from the photons, not the C atoms, so you can turn the arc on only long enough to get a very thin C layer, then let everything cool down (Check that the vacuum returns to what it had been.), and repeat until a sufficiently thick C layer has been built up. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 7, 23 -- From tivol-at-caltech.edu Wed Jul 9 13:24:33 2008 7, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m69IOUqV019885 7, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 13:24:31 -0500 7, 23 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 7, 23 -- by fire-doxen-postvirus (Postfix) with ESMTP id D158F2E50159 7, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 11:24:28 -0700 (PDT) 7, 23 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 7, 23 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 7, 23 -- by fire-doxen-ssl (Postfix) with ESMTP id 0AC992E500B4 7, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 11:24:23 -0700 (PDT) 7, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 7, 23 -- In-Reply-To: {200807070048.m670mFPp024635-at-ns.microscopy.com} 7, 23 -- References: {200807070048.m670mFPp024635-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; format=flowed 7, 23 -- Message-Id: {2123B045-CDD9-437F-BEA9-6867C6931D6D-at-caltech.edu} 7, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 7, 23 -- Subject: Re: [Microscopy] TEM stabilization layer for delicate polymers 7, 23 -- Date: Wed, 9 Jul 2008 11:24:22 -0700 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- X-Mailer: Apple Mail (2.753.1) 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m69IOUqV019885 ==============================End of - Headers==============================
} I have an EM specimen which only stabilizes in high salt buffer (1M } NaCl). I would like to carry out cryoEM imaging at lower salt } conditions. Does anyone have a good protocol to reduce salt to } 100mM or less without compromising the specimen? } Hi Peijun, When we tried to reduce the salt content that an extremophyle lived in, we always disrupted the bug due to osmotic forces, so, if your specimen is stable only in 1 M salt, you are unlikely to be able to reduce the ionic strength or osmolarity without disrupting the specimen. You can, however, reduce the average Z by replacing NaCl with another salt, such as LiF, or you could try NH4HCO3. No guarantees at all as to whether your specimen is stable in either of these conditions. If you choose LiF, be careful. F- is toxic in large amounts. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Wed Jul 9 13:25:11 2008 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m69IPA9M020065 4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 13:25:10 -0500 4, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 4, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 972DA2E5010C 4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 11:25:09 -0700 (PDT) 4, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 4, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 4, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 91F492E500B4 4, 22 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 11:25:08 -0700 (PDT) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 4, 22 -- In-Reply-To: {007f01c8e07e$ffb210a0$6701a8c0-at-peijun01} 4, 22 -- References: {007f01c8e07e$ffb210a0$6701a8c0-at-peijun01} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 22 -- Message-Id: {E87A8649-B49F-4342-9CD5-D36971CD512F-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [3dem] EM sample in high salt 4, 22 -- Date: Wed, 9 Jul 2008 11:25:07 -0700 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Since many JEOL 200, 2000 and 4000 TEMs have been taken out of service in the last 5 years, does anyone have a spare Wehnelt assembly from one of these? We would like to procure a spare and are hoping there might be some freebies around.
Thanks, Roseann
Roseann Csencsits, PhD LBL Donner Lab 365 1 Cyclotron Road Berkeley CA 94720 United States 510-486-4548
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Email: eak-at-uoregon.edu Name: elizabeth
Organization: university of oregon
Title-Subject: [Filtered] photoscope
Question: I need help with the microscopy camera software Photoscope. Is anyone familiar with this software? We photograph textiles and other fibers under the scope, but can't seem to figure out how to use the software for measurements, ect. The manual is not helpful.
Dear listers, I wanted to react to this frightening message from Paula (I know it was ironic, but still...). "We work with enough things that are carcinogenic, mutagenic, teratogenic or will kill you outright." With all respect, there seems to be a culture of terror in the EM labs and I think it would be good to make the point with the products we use everyday. Glutaraldehyde: Toxic by contact but not carcinogenic. Simply dont touch it, dont drink it. Which makes sense. Formaldehyde: really bad. Take care. For this one you are right ;-) Osmium tetroxide: very toxic but not carcinogenic. EPON (components): some are toxic (very toxic) but they are not carcinogenic Ethanol: dont drink it! Acetone: used to clean nails Scalpels: take care of your fingertips. Not carcinogenic. All in all, working in a fume hood with gloves, which really makes sense in a modern lab, is enough to keep you safe. Much more difficult to avoid are dangers due to human behavior: talking excessively or thinking about your last rendezvous with Nicole Kidman/Will Smith are not welcome while handling dangerous products. A short note on the toxicity of formaldehyde for example: it is generally used at a max. conc. of 4%, this is 4g/100 ml. Let's assume you spill 1 ml on your skin (realistically speaking it is unprobable to have more than 50 µl but lets be unrealistic), these represent 40 mg. For a body weight of 60 kg (90 kg in my case :-)). If you wash immediately, the effective amount penetrating the body is probably ridiculous compared to the bad substances you ingest when eating potato chips or grilled meat. Conclusion: be careful, not paranoid (but the security people usually think just the contrary :-D). And stop eating grilled meat. Best regards,
Stephane
----- Original Message ---- X-from: "vapatpxs-at-yahoo.com" {vapatpxs-at-yahoo.com} To: nizets2-at-yahoo.com Sent: Tuesday, July 8, 2008 6:26:28 PM
Hello Listers,
After receiving many replies the consensus seems to be----propylene oxide is not necessary. Some people still keep it around if they have tissue that is difficult to infiltrate.
Several people dehydrate in ETOH and then transition into Acetone and use Acetone as the intermediate solvent for infiltration.
Other people just use either Ethanol or Acetone for the entire dehydration and infiltration.
So if you, like me have safety people giving the evil eye to your PO and you don't want to work with an additional toxic, nasty chemical, you don't have to use PO.
We work with enough things that are carcinogenic, mutagenic, teratogenic or will kill you outright. It's nice to be able to eliminate at least one of the icky chemicals.
I just thought I'd let you know. Also, thanks to all who replied.
Paula :-)
Paula Sicurello VA San Diego Healthcare System Microscope Facility room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 12, 24 -- From vapatpxs-at-yahoo.com Tue Jul 8 11:20:05 2008 12, 24 -- Received: from n63.bullet.mail.sp1.yahoo.com (n63.bullet.mail.sp1.yahoo.com [98.136.44.33]) 12, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m68GK5d4004479 12, 24 -- for {microscopy-at-microscopy.com} ; Tue, 8 Jul 2008 11:20:05 -0500 12, 24 -- Received: from [216.252.122.216] by n63.bullet.mail.sp1.yahoo.com with NNFMP; 08 Jul 2008 16:20:04 -0000 12, 24 -- Received: from [69.147.65.169] by t1.bullet.sp1.yahoo.com with NNFMP; 08 Jul 2008 16:20:04 -0000 12, 24 -- Received: from [127.0.0.1] by omp504.mail.sp1.yahoo.com with NNFMP; 08 Jul 2008 16:20:04 -0000 12, 24 -- X-Yahoo-Newman-Property: ymail-3 12, 24 -- X-Yahoo-Newman-Id: 843885.41428.bm-at-omp504.mail.sp1.yahoo.com 12, 24 -- Received: (qmail 12599 invoked by uid 60001); 8 Jul 2008 16:20:02 -0000 12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 24 -- s=s1024; d=yahoo.com; 12, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 12, 24 -- b=b+5iNDzxqN2pAmQ1PjKOELvvs5/FUUmFEDfNSYWt/vgBq3QK5D1uQgcKnnSMWSS6R2l65V0VcbcnzNp5V3ZY3dz6s9gB/rNLv9rnTJiu972+bo26hl64ax5rGLMlhmz4eU2cI6zKb09+sW5nHaKKOOsuef0Vc/VILBBYw5Oldzk=; 12, 24 -- Received: from [132.239.85.200] by web46109.mail.sp1.yahoo.com via HTTP; Tue, 08 Jul 2008 09:20:02 PDT 12, 24 -- X-Mailer: YahooMailWebService/0.7.199 12, 24 -- Date: Tue, 8 Jul 2008 09:20:02 -0700 (PDT) 12, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 12, 24 -- Reply-To: vapatpxs-at-yahoo.com 12, 24 -- Subject: Propylene Oxide vs Ethanol or Acetone results 12, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 12, 24 -- MIME-Version: 1.0 12, 24 -- Content-Type: text/plain; charset=us-ascii 12, 24 -- Message-ID: {386574.10983.qm-at-web46109.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 25, 20 -- From nizets2-at-yahoo.com Thu Jul 10 02:34:53 2008 25, 20 -- Received: from web37401.mail.mud.yahoo.com (web37401.mail.mud.yahoo.com [209.191.91.133]) 25, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6A7Yr2h029387 25, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jul 2008 02:34:53 -0500 25, 20 -- Received: (qmail 54380 invoked by uid 60001); 10 Jul 2008 07:34:53 -0000 25, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 25, 20 -- s=s1024; d=yahoo.com; 25, 20 -- h=Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 25, 20 -- b=2x+9MZCddb46LuxXV08E28U3PY4N0fvk+Je73iU7k4Z5c6GfXUf4sosJKxWAOQjad1UYEOqEtBfaNbnf0EtZ6ZTpsaUndcarN59PsIeQ6QjZkHsPd1MWPk2yoN17Fk/+YleTreWTQT0cqJSZQvGsh/5yYkuIZLegc3Hx47XzqpE=; 25, 20 -- Received: from [80.122.101.100] by web37401.mail.mud.yahoo.com via HTTP; Thu, 10 Jul 2008 00:34:53 PDT 25, 20 -- X-Mailer: YahooMailRC/975.45 YahooMailWebService/0.7.199 25, 20 -- Date: Thu, 10 Jul 2008 00:34:53 -0700 (PDT) 25, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 25, 20 -- Subject: Evil products of EM 25, 20 -- To: microscopy-at-microscopy.com 25, 20 -- MIME-Version: 1.0 25, 20 -- Content-Type: text/plain; charset=iso-8859-1 25, 20 -- Message-ID: {459097.54105.qm-at-web37401.mail.mud.yahoo.com} 25, 20 -- Content-Transfer-Encoding: 8bit 25, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6A7Yr2h029387 ==============================End of - Headers==============================
Hi Ursula! You didnt mention osmium fixation?! Uranyl can precipitate, but the precipitate don't match with your description of "very dense particles up to about 60 nm). I would advise (1) to try without uranyl en bloc staining (2) analyse the precipitates by EDX or EELS would the best way to identify them. Regards,
Stephane
----- Original Message ---- X-from: "U.J.Potter-at-bath.ac.uk" {U.J.Potter-at-bath.ac.uk} To: nizets2-at-yahoo.com Sent: Wednesday, July 9, 2008 3:10:39 PM
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Email: U.J.Potter-at-bath.ac.uk Name: Ursula Potter
Organization: University of Bath UK
Title-Subject: [Filtered] Black precipitate in sperm TEM sections
Question: Dear All,
I have been preparing Drosophila sperm in and out of seminal vesicles and found that a black precipitate is present in the tissue. It is either fine grain particles or very dense particles up to about 60nm in size. Resin only areas of a section are clean.
The tissue was prepared using Glutaraldhyde in sodium cacodylate, osmium, encapsulation of pellet in agarose, uranyl acetate block stain, acetone dehydration & embedding in Spurr resin. I used no section staining. The structure of the sperm is great - the precipitate ruins everything! Other tissues I've prepared recently are clear of precipitate.
I would be very grateful for any advice/comments on how to resolve the problem!
Did a little research for you. Photoscope as a microscopy tool doesn't seem to exist on the web except in one place - the advertisement for this microscope from MTI:
This is much more helpful - ScopePhoto seems to be real software, bundled with many microscopes. Turns out it's made by a company in China:
http://www.oplenic.com/products-view.asp?id=69
I'm not greatly surprised that you didn't get much help from the instructions - their English doesn't seem that great. They'd probably be the best for explaining their software, though, and they are apparently the only people to go to for upgrades. They seem to be available through IM, too, if you look at the very bottom of the page, you see links and times for it. If you're budget-limited, I know some measurements can be made easily with a FOSS program called GIMP, and with a higher learning curve, ImageJ. I'd suggest taking the time to learn ImageJ, it can be very powerful.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company 3 Sharpie Way Shelbyville, TN 37160 Direct: +1 (931) 685-6635 Fax: +1 (931) 685-6613 robert.zonis-at-sanford.com www.papermate.com www.sharpie.com
-----Original Message----- X-from: eak-at-uoregon.edu [mailto:eak-at-uoregon.edu] Sent: Wednesday, July 09, 2008 8:47 PM To: Zonis, Robert
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both eak-at-uoregon.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: eak-at-uoregon.edu Name: elizabeth
Organization: university of oregon
Title-Subject: [Filtered] photoscope
Question: I need help with the microscopy camera software Photoscope. Is anyone familiar with this software? We photograph textiles and other fibers under the scope, but can't seem to figure out how to use the software for measurements, ect. The manual is not helpful.
==============================Original Headers============================== 7, 11 -- From zaluzec-at-microscopy.com Wed Jul 9 20:40:33 2008 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6A1eWMl007514 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 9 Jul 2008 20:40:33 -0500 7, 11 -- Mime-Version: 1.0 7, 11 -- Message-Id: {p06240801c49b190371f0-at-[206.69.208.22]} 7, 11 -- Date: Wed, 9 Jul 2008 20:40:32 -0500 7, 11 -- To: microscopy-at-microscopy.com 7, 11 -- From: eak-at-uoregon.edu (by way of MicroscopyListserver) 7, 11 -- Subject: viaWWW: photoscope 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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A really good mixture for electropolishing steels for TEM is acetic acid/perchloric acid/glycerol - as long as you keep it cold, it won't go bang - honest.
HF is very good for preparing all sorts of ceramics, glasses, etc - just make sure you have the hypodermics with calcium gluconate (?) handy ..... although, I never actually saw anybody having to stick the needle into their knuckles.
Hot cyanide solutions are very effective for dissolving gold ........ I think there's an antidote for that ......
My favourite is liquid nitrogen - I have been reprimanded numerous times in the past for my habit of wearing sandles while handling liquid nitrogen - by people wearing shoes. I've always challenged them to put their shoed foot beside my sandled foot and pour liquid nitrogen over both - first person to tear their footwear off looses .....! None would accept the suggestion.
In the wider world, do these safey people really undestand how flammable petrol (gas) is? And every day, millions of people simply pump millions of gallons into their cars - very dangerous - should be banned.
Recently purchased a new oven - 3 pages at the back of the instruction book explaining how charring food could lead to cancer and if the instructions were followed correctly, there would be no dangerous, cancerous black regions on the food this wonderful oven cooked .....
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==============================Original Headers============================== 11, 16 -- From larry-at-cymru666.plus.com Thu Jul 10 14:55:05 2008 11, 16 -- Received: from pih-relay08.plus.net (pih-relay08.plus.net [212.159.14.134]) 11, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6AJt514029302 11, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Jul 2008 14:55:05 -0500 11, 16 -- Received: from [87.115.74.88] (helo=[192.168.1.2]) 11, 16 -- by pih-relay08.plus.net with esmtp (Exim) id 1KH2EJ-0004Z7-LW 11, 16 -- for Microscopy-at-microscopy.com; Thu, 10 Jul 2008 20:55:05 +0100 11, 16 -- Mime-Version: 1.0 11, 16 -- Message-Id: {p06240804c49c1986042f-at-[192.168.1.2]} 11, 16 -- Date: Thu, 10 Jul 2008 20:54:53 +0100 11, 16 -- To: Microscopy-at-microscopy.com 11, 16 -- From: Larry Stoter {larry-at-cymru666.plus.com} 11, 16 -- Subject: Re: [Microscopy] Evil products of EM 11, 16 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 11, 16 -- Content-Transfer-Encoding: 8bit 11, 16 -- X-Plusnet-Relay: 4b2d5f02d86deb6b37d2abc52b54cae4 ==============================End of - Headers==============================
} For me chocolate is an evil thing because it makes me fat! } } Actually, everyone should practice standard safety } precautions with all aspects of lab work. I've had } people spill acrylimide powder all over a balance and then } leave it, thank goodness for premade gells. } } I've also had students who don't like the fumehoods } and want to open sample vials with osmium in them right up } close to their face. One kid wanted to see why his } sample had turned black up close and personal. I have } spilled osmium in the past and the safety people and the fire } departments hazardous materials unit showed up in self } contained breathing suits to clean it up. } } Common sense all around does a world of good. } } Also, stay away from nail salons (fiberglass, xylene and } toluene based nail polish, the dreaded acetone etc) and don't eat things like hotdogs (loaded with carcinogenic nitrates). Especially don't eat hotdogs while getting your nails done. ;) } } Ciao, } } Paula :-) } } Paula Sicurello } VA San Diego Healthcare System } Microscope Facility room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } --- On Thu, 7/10/08, nizets2-at-yahoo.com } {nizets2-at-yahoo.com} wrote: } } } From: nizets2-at-yahoo.com {nizets2-at-yahoo.com} } } Subject: [Microscopy] Evil products of EM } } To: vapatpxs-at-yahoo.com } } Date: Thursday, July 10, 2008, 7:45 AM } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy } } Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Dear listers, } } I wanted to react to this frightening message from } Paula (I } } know it was ironic, but still...). } } "We work with enough things that are } carcinogenic, } } mutagenic, teratogenic or will kill you } outright." } } With all respect, there seems to be a culture of } terror in } } the EM labs and I think it would be good to make the } point } } with the products we use everyday. } } Glutaraldehyde: Toxic by contact but not carcinogenic. } } Simply dont touch it, dont drink it. Which makes } sense. } } Formaldehyde: really bad. Take care. For this one you } are } } right ;-) } } Osmium tetroxide: very toxic but not carcinogenic. } } EPON (components): some are toxic (very toxic) but } they are } } not carcinogenic } } Ethanol: dont drink it! } } Acetone: used to clean nails } } Scalpels: take care of your fingertips. Not } carcinogenic. } } All in all, working in a fume hood with gloves, which } } really makes sense in a modern lab, is enough to keep } you } } safe. } } Much more difficult to avoid are dangers due to human } } behavior: talking excessively or thinking about your } last } } rendezvous with Nicole Kidman/Will Smith are not } welcome } } while handling dangerous products. } } A short note on the toxicity of formaldehyde for } example: } } it is generally used at a max. conc. of 4%, this is } 4g/100 } } ml. Let's assume you spill 1 ml on your skin } } (realistically speaking it is unprobable to have more } than } } 50 µl but lets be unrealistic), these represent 40 } mg. } } For a body weight of 60 kg (90 kg in my case :-)). If } you } } wash immediately, the effective amount penetrating } the } } body is probably ridiculous compared to the bad } substances } } you ingest when eating potato chips or grilled meat. } } Conclusion: be careful, not paranoid (but the security } } people usually think just the contrary :-D). And stop } } eating grilled meat. } } Best regards, } } } } Stephane } } } } } } ----- Original Message ---- } } X-from: "vapatpxs-at-yahoo.com" } } {vapatpxs-at-yahoo.com} } } To: nizets2-at-yahoo.com } } Sent: Tuesday, July 8, 2008 6:26:28 PM } } Subject: [Microscopy] Propylene Oxide vs Ethanol or } Acetone } } results } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy } } Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hello Listers, } } } } After receiving many replies the consensus seems to } } be----propylene oxide is not necessary. Some people } still } } keep it around if they have tissue that is difficult } to } } infiltrate. } } } } Several people dehydrate in ETOH and then transition } into } } Acetone and use Acetone as the intermediate solvent } for } } infiltration. } } } } Other people just use either Ethanol or Acetone for } the } } entire dehydration and infiltration. } } } } So if you, like me have safety people giving the evil } eye } } to your PO and you don't want to work with an } } additional toxic, nasty chemical, you don't have } to use } } PO. } } } } We work with enough things that are carcinogenic, } } mutagenic, teratogenic or will kill you outright. } } It's nice to be able to eliminate at least one of } the } } icky chemicals. } } } } I just thought I'd let you know. Also, thanks to } all } } who replied. } } } } Paula :-) } } } } Paula Sicurello } } VA San Diego Healthcare System } } Microscope Facility room B141 } } 3350 La Jolla Village Dr., MC151 } } San Diego, CA 92161 } } 858-552-8585 x2397 } } } } } } } } } } } } ==============================Original } } Headers============================== } } 12, 24 -- From vapatpxs-at-yahoo.com Tue Jul 8 11:20:05 } 2008 } } 12, 24 -- Received: from n63.bullet.mail.sp1.yahoo.com } } (n63.bullet.mail.sp1.yahoo.com [98.136.44.33]) } } 12, 24 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with SMTP id m68GK5d4004479 } } 12, 24 -- for {microscopy-at-microscopy.com} ; } } Tue, 8 Jul 2008 11:20:05 -0500 } } 12, 24 -- Received: from [216.252.122.216] by } } n63.bullet.mail.sp1.yahoo.com with NNFMP; 08 Jul 2008 } } 16:20:04 -0000 } } 12, 24 -- Received: from [69.147.65.169] by } } t1.bullet.sp1.yahoo.com with NNFMP; 08 Jul 2008 } 16:20:04 } } -0000 } } 12, 24 -- Received: from [127.0.0.1] by } } omp504.mail.sp1.yahoo.com with NNFMP; 08 Jul 2008 } 16:20:04 } } -0000 } } 12, 24 -- X-Yahoo-Newman-Property: ymail-3 } } 12, 24 -- X-Yahoo-Newman-Id: } } 843885.41428.bm-at-omp504.mail.sp1.yahoo.com } } 12, 24 -- Received: (qmail 12599 invoked by uid } 60001); 8 } } Jul 2008 16:20:02 -0000 } } 12, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; } c=nofws; } } 12, 24 -- s=s1024; d=yahoo.com; } } 12, 24 -- } } } h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; } } 12, 24 -- } } } b=b+5iNDzxqN2pAmQ1PjKOELvvs5/FUUmFEDfNSYWt/vgBq3QK5D1uQgcKnnSMWSS6R2l65V0VcbcnzNp5V3ZY3dz6s9gB/rNLv9rnTJiu972+bo26hl64ax5rGLMlhmz4eU2cI6zKb09+sW5nHaKKOOsuef0Vc/VILBBYw5Oldzk=; } } 12, 24 -- Received: from [132.239.85.200] by } } web46109.mail.sp1.yahoo.com via HTTP; Tue, 08 Jul 2008 } } 09:20:02 PDT } } 12, 24 -- X-Mailer: YahooMailWebService/0.7.199 } } 12, 24 -- Date: Tue, 8 Jul 2008 09:20:02 -0700 (PDT) } } 12, 24 -- From: Va Paula Sicurello } } {vapatpxs-at-yahoo.com} } } 12, 24 -- Reply-To: vapatpxs-at-yahoo.com } } 12, 24 -- Subject: Propylene Oxide vs Ethanol or } Acetone } } results } } 12, 24 -- To: MSA BB {Microscopy-at-microscopy.com} } } 12, 24 -- MIME-Version: 1.0 } } 12, 24 -- Content-Type: text/plain; charset=us-ascii } } 12, 24 -- Message-ID: } } {386574.10983.qm-at-web46109.mail.sp1.yahoo.com} } } ==============================End of - } } Headers============================== } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } 25, 20 -- From nizets2-at-yahoo.com Thu Jul 10 02:34:53 } 2008 } } 25, 20 -- Received: from web37401.mail.mud.yahoo.com } } (web37401.mail.mud.yahoo.com [209.191.91.133]) } } 25, 20 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) } } with SMTP id m6A7Yr2h029387 } } 25, 20 -- for {microscopy-at-microscopy.com} ; Thu, } 10 } } Jul 2008 02:34:53 -0500 } } 25, 20 -- Received: (qmail 54380 invoked by uid } 60001); 10 } } Jul 2008 07:34:53 -0000 } } 25, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; } c=nofws; } } 25, 20 -- s=s1024; d=yahoo.com; } } 25, 20 -- } } } h=Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; } } 25, 20 -- } } } b=2x+9MZCddb46LuxXV08E28U3PY4N0fvk+Je73iU7k4Z5c6GfXUf4sosJKxWAOQjad1UYEOqEtBfaNbnf0EtZ6ZTpsaUndcarN59PsIeQ6QjZkHsPd1MWPk2yoN17Fk/+YleTreWTQT0cqJSZQvGsh/5yYkuIZLegc3Hx47XzqpE=; } } 25, 20 -- Received: from [80.122.101.100] by } } web37401.mail.mud.yahoo.com via HTTP; Thu, 10 Jul 2008 } } 00:34:53 PDT } } 25, 20 -- X-Mailer: YahooMailRC/975.45 } } YahooMailWebService/0.7.199 } } 25, 20 -- Date: Thu, 10 Jul 2008 00:34:53 -0700 (PDT) } } 25, 20 -- From: Stephane Nizet } {nizets2-at-yahoo.com} } } 25, 20 -- Subject: Evil products of EM } } 25, 20 -- To: microscopy-at-microscopy.com } } 25, 20 -- MIME-Version: 1.0 } } 25, 20 -- Content-Type: text/plain; charset=iso-8859-1 } } 25, 20 -- Message-ID: } } {459097.54105.qm-at-web37401.mail.mud.yahoo.com} } } 25, 20 -- Content-Transfer-Encoding: 8bit } } 25, 20 -- X-MIME-Autoconverted: from quoted-printable } to } } 8bit by ns.microscopy.com id m6A7Yr2h029387 } } ==============================End of - } } Headers==============================
==============================Original Headers============================== 8, 26 -- From vapatpxs-at-yahoo.com Thu Jul 10 15:33:04 2008 8, 26 -- Received: from n55.bullet.mail.sp1.yahoo.com (n55.bullet.mail.sp1.yahoo.com [98.136.44.188]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6AKX4re010745 8, 26 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jul 2008 15:33:04 -0500 8, 26 -- Received: from [216.252.122.218] by n55.bullet.mail.sp1.yahoo.com with NNFMP; 10 Jul 2008 20:33:03 -0000 8, 26 -- Received: from [69.147.65.153] by t3.bullet.sp1.yahoo.com with NNFMP; 10 Jul 2008 20:33:03 -0000 8, 26 -- Received: from [127.0.0.1] by omp401.mail.sp1.yahoo.com with NNFMP; 10 Jul 2008 20:33:03 -0000 8, 26 -- X-Yahoo-Newman-Property: ymail-5 8, 26 -- X-Yahoo-Newman-Id: 961580.96396.bm-at-omp401.mail.sp1.yahoo.com 8, 26 -- Received: (qmail 2827 invoked by uid 60001); 10 Jul 2008 20:33:03 -0000 8, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 8, 26 -- s=s1024; d=yahoo.com; 8, 26 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 8, 26 -- b=O4vwRypTrRvrfIbomqn7UBhj8bqNmsn7yzEO4Akkcsh2n71o2CPjUqZGExN+ngAog5r7RxAkNvY09yPjk+/iIX1+oyUEvaTBGH2kS3KsusOJ/Yfps8xOnfvEuQ8ThIOi+uORFcoLln6yHrxPt13avCUNj/E5L8wEbJ6LR9AaIV0=; 8, 26 -- Received: from [132.239.85.200] by web46105.mail.sp1.yahoo.com via HTTP; Thu, 10 Jul 2008 13:33:03 PDT 8, 26 -- X-Mailer: YahooMailWebService/0.7.199 8, 26 -- Date: Thu, 10 Jul 2008 13:33:03 -0700 (PDT) 8, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 8, 26 -- Reply-To: vapatpxs-at-yahoo.com 8, 26 -- Subject: Re: [Microscopy] Evil products of EM 8, 26 -- To: MSA BB {Microscopy-at-microscopy.com} 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; charset=iso-8859-1 8, 26 -- Message-ID: {519367.2251.qm-at-web46105.mail.sp1.yahoo.com} 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6AKX4re010745 ==============================End of - Headers==============================
I would like to thank you for putting things somewhat into perspective. While things are not necessarily baby's play toys, reasonable precautions are what we really need to protect ourselves. And most of us do apply reasonable precautions - I think...
My only problem with your note, though, is the admonishments against drinking the ethanol, eating the chips, and grilling meat. :'(
paul
--
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 7, 20 -- From paul_hazelton-at-umanitoba.ca Thu Jul 10 15:38:30 2008 7, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6AKcSBQ022202 7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jul 2008 15:38:30 -0500 7, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 7, 20 -- (authenticated bits=0) 7, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m6AKcRah006227; 7, 20 -- Thu, 10 Jul 2008 15:38:27 -0500 (CDT) 7, 20 -- Message-ID: {4876733F.4050503-at-umanitoba.ca} 7, 20 -- Date: Thu, 10 Jul 2008 15:38:23 -0500 7, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 20 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 7, 20 -- MIME-Version: 1.0 7, 20 -- To: nizets2-at-yahoo.com, Microscopy Listserver {microscopy-at-microscopy.com} 7, 20 -- Subject: Re: [Microscopy] Evil products of EM 7, 20 -- References: {200807100737.m6A7bMTh032331-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200807100737.m6A7bMTh032331-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
As I promised I'm sharing our experiences about contrasting problem: We definitely figured out that the problem was due to uranyl acetate. We tried your advices and stained our sections with only uranyl, only lead and also tried without staining. Our results were clear that precipitates are due to the uranyl acetate step. We also tried staining with modifying UA step (more careful manipulations) and results were great. I'd like to thank all of my colleagues interested helpfully with our contrast problem. King Regards
Necat Yilmaz
==============================Original Headers============================== 4, 31 -- From nyilmaz-at-mersin.edu.tr Thu Jul 10 15:53:15 2008 4, 31 -- Received: from mail.mersin.edu.tr ([193.255.128.97]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6AKrE0F004426 4, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Jul 2008 15:53:14 -0500 4, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id E4467227E8B; 4, 31 -- Thu, 10 Jul 2008 23:50:14 +0300 (EEST) 4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 4, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 4, 31 -- with ESMTP id BlDNWPTj6TVj; Thu, 10 Jul 2008 23:49:44 +0300 (EEST) 4, 31 -- Received: from nejat1 (unknown [88.229.140.41]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id B2D43227E87; 4, 31 -- Thu, 10 Jul 2008 23:49:36 +0300 (EEST) 4, 31 -- Message-ID: {001401c8e2ce$e214bf50$0301a8c0-at-nejat1} 4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 4, 31 -- Subject: Happy End (does staining artifact occur due to section thickness) 4, 31 -- Date: Thu, 10 Jul 2008 23:52:34 +0300 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; 4, 31 -- format=flowed; 4, 31 -- charset="iso-8859-9"; 4, 31 -- reply-type=original 4, 31 -- Content-Transfer-Encoding: 7bit 4, 31 -- X-Priority: 3 4, 31 -- X-MSMail-Priority: Normal 4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 4, 31 -- X-Virus-Scanned: ClamAV using ClamSMTP ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rpowell-at-nanoprobes.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rpowell-at-nanoprobes.com Name: Rick Powell
Organization: Nanoprobes, Incorporated
Title-Subject: viaWWW: Osmium precipitates after silver enhancement
Question: [Commercial vendor response]
Hello Chee Yeun and Microscopy Listers:
It's difficult to identify a specific reason for the background in your procedure without more information, so here are a few possibilities:
Generally: to avoid background with silver enhancers, there are some precautions that are recommended:
(1) Ensure that all samples are thoroughly washed with ultrapure or deionized water before silver enhancement: halide or other silver-precipitating anions will cause a precipitate to form, which is itself visible in the EM and will nucleate additional background staining. (2) Wash samples with a chelating agent before silver enhancement. We usually use disodium EDTA (0.05 M, pH 4.6) on blots as the last wash before adding the silver enhancement reagent. (3) Clean glassware (for mixing reagents, handling specimens or storing silver enhancers) with Farmer's solution for at least 20-30 minutes. Farmer's solution consists of 9 parts of 10 % sodium thiosulfate with 1 part of 10 % potassium ferricyanide. (4) Use plastic or teflonized (not metal) forceps and tools for handling specimens.
Washing with sodium citrate buffer before silver enhancement resulted in lower background than other buffers in our experiments with FluoroNanogold: wash 2 x 3 minutes with 0.02 M sodium citrate buffer, pH 7.0, before applying HQ Silver (or 0.02 M sodium citrate, pH 3.5, before Danscher formulation silver enhancer). This probably helps to chelate and remove any redox-active transition metal ions that might catalyze silver deposition. Likewise, washing with 0.05 M disodium EDTA (pH 4.6) before silver enhancement produces lower background on blots, possibly for the same reason.
Reference:
Powell, R. D.; Halsey, C. M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous" detection of a pre-mRNA splicing factor by light and electron microscopy. J. Histochem. Cytochem., 45, 947-956 (1997).
If possible, reducing the proportion of glutaraldehyde may also help, possibly because it makes removal of the silver enhancement solution easier after silver enhancement is complete.
Several post-treatments ("stop" reactions) have also been reported to help reduce background; these stop the silver enhancement reaction within the specimen. These include:
(1) 1% acetic acid. (2) 1% acetic acid followed by photographic fixer (Agefix, Agfa-Gevaert, or Ilfospeed 200, Ilford). (3) direct photo fix, using those just mentioned. (4) brief rinse in 2.5% sodium chloride. (5) 15-25% aqueous sodium thiosulfate plus 15% sodium sulfite. (6) 1% acetic acid, washes in acetate buffer, toning in 0.05% HAuCl4 3-10 min, with excess silver removed with 3% sodium thiosulfate. We found that Nanogold-labeled proteins on a polyacrylamide gel produced low backgrounds when stopped with 10% acetic acid with 10% glucose in water, as opposed to just a water stop.
Addition of a small amount (0.1%) of Tween-20 detergent to the Nanogold incubation buffer and / or to the wash buffer used after the Nanogold, helps to prevent hydrophobic interactions, which can be another source of non-specific binding. If the sample can tolerate it, washing with 0.6 M triethylammonium bicarbonate buffer, in which Nanogold is highly soluble, can also lower background. Reference for preparation of triethylammonium bicarbonate buffer:
Safer, D.; Bolinger, L., and Leigh, J. S.: Undecagold clusters for site-specific labeling of biological macromolecules: simplified preparation and model applications. J. Inorg. Biochem., 26, 77-91 (1986).
Finally, an alternative approach is to "back-develop" to remove the excess background staining by treatment with Farmer's solution (0.3 ml 7.5% potassium ferricyanide, 1.2 mL of 20% sodium thiosulfate, 60 mL water). Application of this solution briefly to your sample before gold toning may help to remove background silver deposition. Reference:
Danscher, G.: Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electronmicroscopy. Histochemistry, 71, 1-16 (1981).
You might also consider gold enhancement, which under come circumstances produces cleaner backgrounds. Including 1% nonfat dried milk and 0.1 % Tween-20 with the Nanogold incubation buffer, and using as the washing buffer TBS-Tween 20 (20 mM Tris pH 7.6, with 150 mM NaCl and 0.1 % Tween-20) produces significantly lower backgrounds on blots and may well translate into lower backgrounds (and higher particle contrast) in the EM (http://www.nanoprobes.com/Vol9_Iss6.html#2).
Regards,
Rick Powell
********************************************************** NANOPROBES, Incorporated 95 Horse Block Road, Yaphank, NY 11980-9710, USA 1-877-447-6266 * (631) 205-9490 * www.nanoprobes.com **********************************************************
Quoting cychung-at-mclean.harvard.edu:
} } } Email: cychung-at-mclean.harvard.edu } Name: Chee Yeun } } Organization: Harvard Medical School } } Title-Subject: [Filtered] Osmium precipitates after silver enhancement } } Question: I have been optimizing immunostaining procedure for EM } using nanogold and HQ silver in rat tissue perfused with 4% PFA and } 0.5 % glutaraldehyde. It turned out that after osmication (down to } 0.25%, 30min), we get fine precipitates (~5~10 nm) that interferes } with our staining. We tried to lower osmium concentrations to 0.1%, } which removed precipitates but very poor contrast which we cannot } use. I was wondering if you have experienced anything like this and } happen to know good ways to avoid this. }
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] TEM negative to digital conversion
Question: I have had a request from a user to convert several old TEM negatives to a digital format. Normally I use a standard flat-bed scanner for this which produces reasonable (although lower resolution) negative and positive digital image conversion. The user in question would like to try for a higher resolution image conversion than I can provide. Does anyone have a suggested method for negative conversion other than a flat-bed scanner?
Thanks in advance.
Tom Williams
Thomas J. Williams, PhD Center for Electron Microscopy and Microanalysis University of Idaho Moscow, ID 83844-3025 (208) 885-6785 tomw-at-uidaho.edu
for those interested, you can access the program for the M&M Pre-Meeting Congress on "Contrast Interpretation in an Aberration-Free Environment" on the www.microscopy.org web site, under Pre-Meeting Congress.
Just FYI...
Larry -- Dr. Lawrence F. Allard Distinguished Research Staff Member High Temperature Materials Laboratory Microscopy Group Materials Science and Technology Division Oak Ridge National Laboratory 1 Bethel Valley Road PO Box 2008 Oak Ridge, TN 37831-6064 (note: the last 4 lines are sufficient for mailing or overnight courier service) 865-574-4981 (office) 865-607-1144 (cell) 865-576-5413 (fax) http://www.ms.ornl.gov/htmlhome/mauc
==============================Original Headers============================== 4, 22 -- From allardlfjr-at-ornl.gov Thu Jul 10 21:36:07 2008 4, 22 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6B2a7qV018561 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jul 2008 21:36:07 -0500 4, 22 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 4, 22 -- by emroute3.ornl.gov (PMDF V6.3-x14 #31501) 4, 22 -- with ESMTP id {0K3T007J5LW6FZ-at-emroute3.ornl.gov} for 4, 22 -- microscopy-at-microscopy.com; Thu, 10 Jul 2008 22:36:06 -0400 (EDT) 4, 22 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 4, 22 -- (PMDF V6.3-x14 #31501) id {0K3T00A01LW6VK-at-emroute3.ornl.gov} for 4, 22 -- microscopy-at-microscopy.com; Thu, 10 Jul 2008 22:36:06 -0400 (EDT) 4, 22 -- Received: from [192.168.1.134] (dyn219176120.dz.ornl.gov [128.219.176.120]) 4, 22 -- by emroute3.ornl.gov (PMDF V6.3-x14 #31501) 4, 22 -- with ESMTP id {0K3T00A2NLVPTA-at-emroute3.ornl.gov} for 4, 22 -- microscopy-at-microscopy.com; Thu, 10 Jul 2008 22:36:06 -0400 (EDT) 4, 22 -- Date: Thu, 10 Jul 2008 22:35:48 -0400 4, 22 -- From: Larry Allard {allardlfjr-at-ornl.gov} 4, 22 -- Subject: Pre-Meeting Congress Program... 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- Message-id: {p06240801c49c76ef5363-at-[192.168.1.134]} 4, 22 -- MIME-version: 1.0 4, 22 -- Content-type: text/plain; format=flowed; charset=us-ascii ==============================End of - Headers==============================
Its not the dpi that is limiting in most TEM images but the range of gray scale pixels. You need a scanner with a high Dmax so you can resolve the details in the really dark (but not truly black) areas.
-----Original Message----- X-from: tomw-at-uidaho.edu [mailto:tomw-at-uidaho.edu] Sent: Thursday, July 10, 2008 7:19 PM To: Phillips, Thomas E.
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] TEM negative to digital conversion
Question: I have had a request from a user to convert several old TEM negatives to a digital format. Normally I use a standard flat-bed scanner for this which produces reasonable (although lower resolution) negative and positive digital image conversion. The user in question would like to try for a higher resolution image conversion than I can provide. Does anyone have a suggested method for negative conversion other than a flat-bed scanner?
Thanks in advance.
Tom Williams
Thomas J. Williams, PhD Center for Electron Microscopy and Microanalysis University of Idaho Moscow, ID 83844-3025 (208) 885-6785 tomw-at-uidaho.edu
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Email: sbras-at-u.washington.edu Name: Scott Braswell
Organization: UW Nanotech User Facility
Title-Subject: [Filtered] Gold Sputter
Question: Our lab uses an SPI sputter coater module with an Au/Pd (60:40) target to prep samples for SEM imaging. We've never run argon to the instrument, instead, we create an O2/N2 plasma with room air. I'm considering running argon to the instrument with the expectation that it wouldn't oxidize the samples.
Would running argon to the sputter coater result in better SEM imaging? What kinds of samples would benefit from this treatment.
Using a scanner is all I know to convert negatives to digital images but it does make a difference as to what scanner you use. Each is different. My first scanner was sent back after a day for it did not perform as the literature claimed. I only wanted the scanner for converting negatives.
I scanned a lot of negatives on the second flat bed at 600dpi on the Color Mode then later changed the image to B&W in photoshop. It gave a better capture than scanning on B&W. I had thought that a B&W scan would have been best until someone suggested that I do the comparison. Give it a try. If it is better, it may please the user.
I just remembered that the captured image was always too dark, so if the user saw that image, before Adjustment, it would not be an acceptable image. Copies of micrographs, which were done on occasion, were much closer to proper density than the negative scans, especially when I remembered to change the scanner cover back to the white opaque insert!
How many dpi can your scanner capture? I tried higher than 600dpi (800dpi if I remember correctly) but it took much longer for each scan, did not look noticeably better unless I chose 1200dpi, the top ability of my scanner, which took "forever" for the scan to run and for publication one must reduce the image to a lower one anyway.
If the user has a lot of negatives to convert, "several" is a questionable amount, you may suggest that he buy a good scanner. There was a big discussion on the Listserv about which scanners were good for converting negatives a while back. This discussion is in the Listserv Archives for you to see if no one replies with the information as to which scanners are currently the best.
Regards, Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {tomw-at-uidaho.edu} } Reply-To: {tomw-at-uidaho.edu} } Date: Thu, 10 Jul 2008 19:24:15 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] viaWWW: TEM negative to digital conversion } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } Email: tomw-at-uidaho.edu } Name: Tom Williams } Organization: University of Idaho } Title-Subject: [Filtered] TEM negative to digital conversion } } Question: I have had a request from a user to convert several old TEM } negatives to a } digital format. Normally I use a standard flat-bed scanner for this which } produces reasonable (although lower resolution) negative and positive } digital image conversion. The user in question would like to try for a } higher resolution image conversion than I can provide. Does anyone have a } suggested method for negative conversion other than a flat-bed scanner? } } Thanks in advance. } } Thomas J. Williams, PhD } Center for Electron Microscopy and Microanalysis } University of Idaho } Moscow, ID 83844-3025 } (208) 885-6785 } tomw-at-uidaho.edu
==============================Original Headers============================== 12, 27 -- From connellyps-at-nhlbi.nih.gov Fri Jul 11 09:43:02 2008 12, 27 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BEh2v5016705 12, 27 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 09:43:02 -0500 12, 27 -- X-IronPortListener: Outbound_SMTP 12, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) 12, 27 -- by nihxway3out.hub.nih.gov with ESMTP; 11 Jul 2008 10:43:02 -0400 12, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 12, 27 -- Fri, 11 Jul 2008 10:42:56 -0400 12, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.170]) with Microsoft Exchange Server HTTP-DAV ; 12, 27 -- Fri, 11 Jul 2008 14:42:55 +0000 12, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 12, 27 -- Date: Fri, 11 Jul 2008 10:42:52 -0400 12, 27 -- Subject: Re: [Microscopy] viaWWW: TEM negative to digital conversion 12, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 12, 27 -- To: {tomw-at-uidaho.edu} 12, 27 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 27 -- Message-ID: {C49CE9AC.2068%connellyps-at-nhlbi.nih.gov} 12, 27 -- Thread-Topic: [Microscopy] viaWWW: TEM negative to digital conversion 12, 27 -- Thread-Index: AcjjZGWMo9/V+E9XEd25cwANk2Yv1A== 12, 27 -- In-Reply-To: {200807110024.m6B0OF3W005603-at-ns.microscopy.com} 12, 27 -- Mime-version: 1.0 12, 27 -- Content-type: text/plain; 12, 27 -- charset="ISO-8859-1" 12, 27 -- X-OriginalArrivalTime: 11 Jul 2008 14:42:56.0422 (UTC) FILETIME=[682F3C60:01C8E364] 12, 27 -- Content-Transfer-Encoding: 8bit 12, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6BEh2v5016705 ==============================End of - Headers==============================
I'm surprised that you have had any usable results at all using ambient atmosphere as the gas. Argon is generally considered essential for sputter coating. The Argon becomes ionized in the applied field and the Ar+ is accelerated to the target (-HV); the relatively heavy Ar+ slams into the target, sputtering the metal from the surface.
So yes I would think you would see an improvement. While you may have a 5 or 10mA current, I would think it is doing very little sputtering and the times you use must be fairly long to get a useful coating. All the time/current tables generally assume Ar as the gas.
Dale
sbras-at-u.washington.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both sbras-at-u.washington.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: sbras-at-u.washington.edu } Name: Scott Braswell } } Organization: UW Nanotech User Facility } } Title-Subject: [Filtered] Gold Sputter } } Question: Our lab uses an SPI sputter coater module with an Au/Pd } (60:40) target to prep samples for SEM imaging. We've never run } argon to the instrument, instead, we create an O2/N2 plasma with room } air. I'm considering running argon to the instrument with the } expectation that it wouldn't oxidize the samples. } } Would running argon to the sputter coater result in better SEM } imaging? What kinds of samples would benefit from this treatment. } } Thanks } } Login Host: 128.95.73.142 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Fri Jul 11 08:49:08 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BDn7oF002125 } 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 08:49:08 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c49d1536902b-at-[206.69.208.22]} } 8, 11 -- Date: Fri, 11 Jul 2008 08:49:07 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: sbras-at-u.washington.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Gold Sputter } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
We have a customer who has grown cells on Thermonox and needs to see them on edge, so I embedded the thermonox in flat molds in Eponate 12. However when sectioning, the thermonox pulled away from the resin. The cells are then just sort of hanging out in space or the edge of the section folds over on itself so the cells are inside the fold. Can anyone recommend a resin or another way to embed the cells so they'll stay embedded and not separate when sectioning? I tried picking them up on formvar but would like to avoid that if possible.
Thanks in advance, Mary Gail Engle
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 University of KY
==============================Original Headers============================== 7, 26 -- From mgengle-at-email.uky.edu Fri Jul 11 11:37:38 2008 7, 26 -- Received: from uksmtp3.uky.edu (uksmtp3.uky.edu [128.163.16.36]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BGbc5S014283 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:37:38 -0500 7, 26 -- Received: from EX7HB03.ad.uky.edu (Ex7hb03.ad.uky.edu [128.163.187.55]) 7, 26 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) 7, 26 -- (No client certificate requested) 7, 26 -- by uksmtp3.uky.edu (Postfix) with ESMTP id 1580B10609E9 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:28:54 -0400 (EDT) 7, 26 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu 7, 26 -- ([128.163.187.55]) with mapi; Fri, 11 Jul 2008 12:37:37 -0400 7, 26 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 7, 26 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 26 -- Date: Fri, 11 Jul 2008 12:37:36 -0400 7, 26 -- Subject: 7, 26 -- Thread-Index: AcjjdG0pU9/j4iVoTVCcxHyLxcdjTQ== 7, 26 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A8570906119D71-at-EX7FM03.ad.uky.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="us-ascii" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6BGbc5S014283 ==============================End of - Headers==============================
Tom, A different approach is to get a copy stand, a light box and a high-end digital camera with a good macro option. This approach is detailed in John Heuser's paper: "How to Convert a Traditional Electron Microscopy Laboratory to Digital Imaging: Follow the 'Middle Road' ", Traffic Vol 1, Issue 8, 2000, pages: 614-621. Once setup it is faster than the scanner. Good luck, Jon
Jon Mulholland, Director Cell Sciences Imaging Facility Beckman Center, B050 Stanford University School of Medicine Stanford, CA 94305-5301
voice 650-725-7532 fax 650-725-4951
http://taltos.stanford.edu
At 07:49 AM 7/11/2008, you wrote:
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==============================Original Headers============================== 9, 21 -- From jwm-at-stanford.edu Fri Jul 11 11:47:52 2008 9, 21 -- Received: from smtp1.stanford.edu (smtp1.Stanford.EDU [171.67.22.28]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BGlpK1027333 9, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:47:52 -0500 9, 21 -- Received: from smtp1.stanford.edu (localhost.localdomain [127.0.0.1]) 9, 21 -- by localhost (Postfix) with SMTP id 997D82D6C61 9, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 09:47:51 -0700 (PDT) 9, 21 -- Received: from mobile-office.stanford.edu (csif-dell.Stanford.EDU [171.65.22.224]) 9, 21 -- by smtp1.stanford.edu (Postfix) with ESMTP id 20988272AD9 9, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 09:47:50 -0700 (PDT) 9, 21 -- Message-Id: {6.2.5.6.2.20080711093736.0264bf50-at-stanford.edu} 9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.5.6 9, 21 -- Date: Fri, 11 Jul 2008 09:47:44 -0700 9, 21 -- To: Microscopy-at-microscopy.com 9, 21 -- From: Jon Mulholland {jwm-at-stanford.edu} 9, 21 -- Subject: Re: [Microscopy] Re: viaWWW: TEM negative to digital conversion 9, 21 -- In-Reply-To: {200807111449.m6BEnhEa029438-at-ns.microscopy.com} 9, 21 -- References: {200807111449.m6BEnhEa029438-at-ns.microscopy.com} 9, 21 -- Mime-Version: 1.0 9, 21 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 9, 21 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Here's what I do. I embed the Thermanox coverslip inverted (cell side down) on a square of ACLAR (bought from Ted Pella or Electron Microscopy Sciences) over a drop of Eponate 12. Then I polymerize this as usual. After polymerization I remove the ACLAR, it peels away with no effort. Then I use a razor blade and remove small rectangles, about 2mm X 3mm, from the coverslip, about 3 or 4 pieces. Don't cut completely through the coverslip or it is nearly impossible to remove from the resin. I then use the corner of the razor blade to peel the resin from the coverslip. Pour Eponate 12 into the regular silicone mold and then individually place the cut rectangles in the end of the mold creating a stack. Polymerize the blocks. When you section them you will get a nice cross section of several cell layers and they will not split apart. Good luck, Jo Dee
~~Jo Dee Fish~~ Research Technologist III The J. David Gladstone Institutes Co-manager Histology and Microscopy Core
Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158
-----Original Message----- X-from: mgengle-at-email.uky.edu [mailto:mgengle-at-email.uky.edu] Sent: Friday, July 11, 2008 9:41 AM To: jfish-at-gladstone.ucsf.edu
Dear Listers,
We have a customer who has grown cells on Thermonox and needs to see them on edge, so I embedded the thermonox in flat molds in Eponate 12. However when sectioning, the thermonox pulled away from the resin. The cells are then just sort of hanging out in space or the edge of the section folds over on itself so the cells are inside the fold. Can anyone recommend a resin or another way to embed the cells so they'll stay embedded and not separate when sectioning? I tried picking them up on formvar but would like to avoid that if possible.
Thanks in advance, Mary Gail Engle
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 University of KY
==============================Original Headers============================== 7, 26 -- From mgengle-at-email.uky.edu Fri Jul 11 11:37:38 2008 7, 26 -- Received: from uksmtp3.uky.edu (uksmtp3.uky.edu [128.163.16.36]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BGbc5S014283 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:37:38 -0500 7, 26 -- Received: from EX7HB03.ad.uky.edu (Ex7hb03.ad.uky.edu [128.163.187.55]) 7, 26 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) 7, 26 -- (No client certificate requested) 7, 26 -- by uksmtp3.uky.edu (Postfix) with ESMTP id 1580B10609E9 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:28:54 -0400 (EDT) 7, 26 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu 7, 26 -- ([128.163.187.55]) with mapi; Fri, 11 Jul 2008 12:37:37 -0400 7, 26 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 7, 26 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 26 -- Date: Fri, 11 Jul 2008 12:37:36 -0400 7, 26 -- Subject: 7, 26 -- Thread-Index: AcjjdG0pU9/j4iVoTVCcxHyLxcdjTQ== 7, 26 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A8570906119D71-at-EX7FM03.ad.uky.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="us-ascii" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6BGbc5S014283 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 28 -- From jfish-at-gladstone.ucsf.edu Fri Jul 11 11:56:03 2008 16, 28 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BGu2eb007875 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:56:02 -0500 16, 28 -- X-IronPort-AV: E=Sophos;i="4.30,346,1212390000"; 16, 28 -- d="scan'208";a="919822" 16, 28 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25]) 16, 28 -- by gmail2.ucsf.edu with ESMTP; 11 Jul 2008 09:56:02 -0700 16, 28 -- Received: from [169.230.76.4] (HELO JFISH) 16, 28 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 4.2.10) 16, 28 -- with ESMTP id 565536600; Fri, 11 Jul 2008 09:56:01 -0700 16, 28 -- Reply-To: {jfish-at-gladstone.ucsf.edu} 16, 28 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu} 16, 28 -- To: {mgengle-at-email.uky.edu} 16, 28 -- Cc: {microscopy-at-microscopy.com} 16, 28 -- References: {200807111641.m6BGfIKU023483-at-ns.microscopy.com} 16, 28 -- Subject: RE: [Microscopy] 16, 28 -- Date: Fri, 11 Jul 2008 09:55:58 -0700 16, 28 -- Organization: J. David Gladstone Institutes 16, 28 -- Message-ID: {000001c8e376$fe225c90$2e0d010a-at-JFISH} 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Type: text/plain; 16, 28 -- charset="us-ascii" 16, 28 -- Content-Transfer-Encoding: 7bit 16, 28 -- X-Mailer: Microsoft Office Outlook 11 16, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 28 -- Thread-Index: AcjjdQqvNnxmC/JuQNa7AjMLUQnDkQAAEwgg 16, 28 -- In-Reply-To: {200807111641.m6BGfIKU023483-at-ns.microscopy.com} ==============================End of - Headers==============================
Since you already have thicker slabs of Epon you can still glue two of them together with some embedding Epon. The best to use is some from the same batch that you used for the original embedding if you have any frozen.
Nearly fill an embedding mold with Epon, put in a small rectangle of your embedded cells (of course chose a dense area) with the cells facing up. Let the Epon flow over the top and place a second rectangle of cells, cell side down in a coverslip like manner to avoid air getting trapped between the layers. Fill the mold as usual. Cure for at least 24 hours - two days would be better since the embedding you have is already fully hard. When you go to section make sure that you are into the area of the embedded cells at the bottom of the block face. Sometimes the layers will separate when trimming the bottom especially if the Epon was not the same. You may need to glass knife trim the last bit instead of razor trimming.
I had presented a similar work at M&M 1977 but it was for cells embedded in dishes. The take home message was that you can embed both control and experimental cells in the same mold and eliminate differences in section thickness and staining if you trim the block in an identifiable manner. Easier to show than to describe! _ | \ | \ ---- Something like this. I put the control on the bottom of the embedding mold and the experimental cells were on top of the control. Trim the side where the controls are perpendicular to the bottom of the block face instead of making a trapezoid. The block face would be at a strong angle on the other side so that you can readily see on the grid which line of cells is which. I have made block faces over 2mm long, but not wide, so that only one section was on each grid.
Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {jfish-at-gladstone.ucsf.edu} } Reply-To: {jfish-at-gladstone.ucsf.edu} } Date: Fri, 11 Jul 2008 11:59:01 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] RE: } } Here's what I do. I embed the Thermanox coverslip inverted (cell side } down) on a square of ACLAR (bought from Ted Pella or Electron Microscopy } Sciences) over a drop of Eponate 12. Then I polymerize this as usual. } After polymerization I remove the ACLAR, it peels away with no effort. Then } I use a razor blade and remove small rectangles, about 2mm X 3mm, from the } coverslip, about 3 or 4 pieces. Don't cut completely through the coverslip } or it is nearly impossible to remove from the resin. I then use the corner } of the razor blade to peel the resin from the coverslip. Pour Eponate 12 } into the regular silicone mold and then individually place the cut } rectangles in the end of the mold creating a stack. Polymerize the blocks. } When you section them you will get a nice cross section of several cell } layers and they will not split apart. } Good luck, } Jo Dee } } ~~Jo Dee Fish~~ } Research Technologist III } The J. David Gladstone Institutes } Co-manager Histology and Microscopy Core } } Telephone: (415) 734-2567 } Fax: (415) 355-0824 } E-mail: jfish-at-gladstone.ucsf.edu } } Mailing address: } The J. David Gladstone Institutes } 1650 Owens Street } San Francisco, CA 94158 } } -----Original Message----- } X-from: mgengle-at-email.uky.edu [mailto:mgengle-at-email.uky.edu] } Sent: Friday, July 11, 2008 9:41 AM } To: jfish-at-gladstone.ucsf.edu } Subject: [Microscopy]
} Dear Listers, } } We have a customer who has grown cells on Thermonox and needs to see them on } edge, so I embedded the thermonox in flat molds in Eponate 12. However when } sectioning, the thermonox pulled away from the resin. The cells are then } just sort of hanging out in space or the edge of the section folds over on } itself so the cells are inside the fold. Can anyone recommend a resin or } another way to embed the cells so they'll stay embedded and not separate } when sectioning? I tried picking them up on formvar but would like to avoid } that if possible. } } Mary Gail Engle } Sr Research Facility Manager } Electron Microscopy & Imaging Facility } HSRB rm 001 } ph (859) 323-6108 } FAX (859) 323-8089 } BBSRB rm o74 } Ph (859)323-2701 } University of KY
==============================Original Headers============================== 12, 26 -- From connellyps-at-nhlbi.nih.gov Fri Jul 11 12:48:11 2008 12, 26 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BHmBNI022432 12, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 12:48:11 -0500 12, 26 -- X-IronPortListener: Outbound_SMTP 12, 26 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) 12, 26 -- by nihxway3out.hub.nih.gov with ESMTP; 11 Jul 2008 13:48:10 -0400 12, 26 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); 12, 26 -- Fri, 11 Jul 2008 13:48:06 -0400 12, 26 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.170]) with Microsoft Exchange Server HTTP-DAV ; 12, 26 -- Fri, 11 Jul 2008 17:48:05 +0000 12, 26 -- User-Agent: Microsoft-Entourage/11.4.0.080122 12, 26 -- Date: Fri, 11 Jul 2008 13:48:03 -0400 12, 26 -- Subject: Re: [Microscopy] RE: TC embedding 12, 26 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 12, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 26 -- Message-ID: {C49D1513.2081%connellyps-at-nhlbi.nih.gov} 12, 26 -- Thread-Topic: [Microscopy] RE: TC embedding 12, 26 -- Thread-Index: AcjjfkQ4grkqSU9xEd25cwANk2Yv1A== 12, 26 -- In-Reply-To: {200807111659.m6BGx1lc015469-at-ns.microscopy.com} 12, 26 -- Mime-version: 1.0 12, 26 -- Content-type: text/plain; 12, 26 -- charset="ISO-8859-1" 12, 26 -- X-OriginalArrivalTime: 11 Jul 2008 17:48:06.0355 (UTC) FILETIME=[46387A30:01C8E37E] 12, 26 -- Content-Transfer-Encoding: 8bit 12, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6BHmBNI022432 ==============================End of - Headers==============================
if the samples are fully embedded then the simplest method is to re- embed the blocks in more of the same resin so that the edge is in the middle of a block and just cut as normal.
Malcolm
Malcolm Haswell e.m. unit University of Sunderland UK
----- Original Message ----- X-from: mgengle-at-email.uky.edu
Hi Tom,
Obviously film scanners are the best way to digitise film, that’s why they are called that - it's the quality of the optics/mechanism that’s paramount for fast hi-res scanning though, coupled to how much you are prepared to pay, and there are lemons out there. You need a flatbed film scanner as you have a large negative size (dedicated 35mm film scanners not being much use unless you get some scissors). Rather than go through all the recent list-server chat from 2007ish on scanning TEM film [which you can find pretty easily I hope), I will send a copy of my recent article on 'scanning film on a tight budget' written for the Royal Microscopy Society's InFocus magazine.
Assuming you are scanning B&W film, scanner DMax isn't as important as scanner resolution (as most modern quality flatbed film scanners have a DMax in excess of that needed for B&W film, it's colour slide film that really needs that high DMax). Unfortunately manufacturer's lie about a scanners DMax and resolution differently so it is difficult to compare. However you can buy a decent flatbed A4 film scanner for under $800 (Epson V750 Pro) and $500 (Epson V700) - see the review link below. The Epson V750 is essentially the same scanner as the V700 but with improved scan optics lenses and other extras like better bundled software (both scan in either film transmission or reflective mode to A4 in size). There don't seem to be any flatbed film scanners to touch these for the [low] price.
Some say scanning in colour and converting to B&W is better [I scan colour slide/negatives mainly], this might be because all the scanners are colour detector ones and Photoshop does a better job of the B&W conversion than the scan driver software. In general leave most things to Photoshop like Image, Adjust, Shadow/Highlight [to bring out detail in shadows on scanned images from colour slide film), and Filter, Sharpen, UnSharp mask (sharpen image), as Photoshop offers far more control. Scanner hardware things like [Kodak] Digital ICE [remove scratches and dust on the film) have to be done by the scanner, but this is cosmetic and isn't designed to work with B&W film anyway (although if you scan in colour mode you can trick it into working, but 'results can vary' in B&W).
See detailed discussion of the cheap V750 and V700 film scanner flatbeds at http://www.photo-i.co.uk/Menus/reviews.htm
These scanners nominally scan at 6,400 dpi max and so produce excellent TEM scan 'working images' at 1200 dpi (in well under a minute) or you can scan smaller areas for enlargement up to 6400 dpi. You shouldn't need to do much post-processing, if any, as 1200 dpi. Use the film holders as distance from the glass platen is critical - check out 3rd party film holders as well. There are more very very expensive scanners that can resolve film better (well the film grain anyway), but these are typically £12,000 to £30,000+ (few, if any, 'cheaper' dedicated 35mm film scanners. E.g. Kodaks, can handle TEM film sizes greater than 9x6cm).
You do need Photoshop CS1 or greater (CS3 is the latest & recommended) as well though, but this isn't that expensive with educational discounts (£130).
Note that scanning at 6,400 dpi produces an enormous image file size [} 100Mb] in TIF format that will be highly prone to data corruption if archived. So keep that original negative as the archived master and don't rely on a digitised hi-res scanned image for long-term archiving. Passing through a second set of optics during scanning will always degrade the image anyway, and some detail will always be lost whatever the scanner used [compared to that seen in the original film negative/colour slide]. The amount of detail lost naturally depends on the scanner quality.
Any queries and just ask.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568
} ----- } Email: tomw-at-uidaho.edu } Name: Tom Williams } Organization: University of Idaho } Title-Subject: [Filtered] TEM negative to digital conversion } } Question: I have had a request from a user to convert several old TEM } negatives to a digital format. Normally I use a standard flat-bed } scanner for this which produces reasonable (although lower resolution) } negative and positive digital image conversion. The user in question } would like to try for a higher resolution image conversion than I can } provide. Does anyone have a suggested method for negative conversion } other than a flat-bed scanner? } } Thanks in advance. } } Thomas J. Williams, PhD } Center for Electron Microscopy and Microanalysis University of Idaho } Moscow, ID 83844-3025 } (208) 885-6785 } tomw-at-uidaho.edu
==============================Original Headers============================== 17, 21 -- From kjmorris-at-well.ox.ac.uk Mon Jul 14 07:49:33 2008 17, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6ECnWgK004360 17, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2008 07:49:33 -0500 17, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 17, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 17, 21 -- id 1KINUi-0003uM-2u 17, 21 -- for Microscopy-at-Microscopy.Com; Mon, 14 Jul 2008 13:49:32 +0100 17, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 17, 21 -- To: {Microscopy-at-Microscopy.Com} 17, 21 -- Subject: [Microscopy] viaWWW: TEM negative to digital conversion 17, 21 -- Date: Mon, 14 Jul 2008 13:49:31 +0100 17, 21 -- Message-ID: {FD4E36A3271943A6809B97F789EDAD6A-at-CytoWhizz} 17, 21 -- MIME-Version: 1.0 17, 21 -- Content-Type: text/plain; 17, 21 -- charset="iso-8859-1" 17, 21 -- X-Mailer: Microsoft Office Outlook 11 17, 21 -- thread-index: AcjlmP6vMrBjHRDYTQe0+oE65a5tFQAC664g 17, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 17, 21 -- Content-Transfer-Encoding: 8bit 17, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6ECnWgK004360 ==============================End of - Headers==============================
if you are going to buy a high-resolution scanner you should definitely consider the Nikon 9000, which, according to reliable sources, outperforms the top of the line models from all competitors in the same price-range by far.
Philip
-----Original Message----- X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk] Sent: 14 July 2008 14:53 To: philip.koeck-at-biosci.ki.se
Hi Tom,
Obviously film scanners are the best way to digitise film, that’s why they are called that - it's the quality of the optics/mechanism that’s paramount for fast hi-res scanning though, coupled to how much you are prepared to pay, and there are lemons out there. You need a flatbed film scanner as you have a large negative size (dedicated 35mm film scanners not being much use unless you get some scissors). Rather than go through all the recent list-server chat from 2007ish on scanning TEM film [which you can find pretty easily I hope), I will send a copy of my recent article on 'scanning film on a tight budget' written for the Royal Microscopy Society's InFocus magazine.
Assuming you are scanning B&W film, scanner DMax isn't as important as scanner resolution (as most modern quality flatbed film scanners have a DMax in excess of that needed for B&W film, it's colour slide film that really needs that high DMax). Unfortunately manufacturer's lie about a scanners DMax and resolution differently so it is difficult to compare. However you can buy a decent flatbed A4 film scanner for under $800 (Epson V750 Pro) and $500 (Epson V700) - see the review link below. The Epson V750 is essentially the same scanner as the V700 but with improved scan optics lenses and other extras like better bundled software (both scan in either film transmission or reflective mode to A4 in size). There don't seem to be any flatbed film scanners to touch these for the [low] price.
Some say scanning in colour and converting to B&W is better [I scan colour slide/negatives mainly], this might be because all the scanners are colour detector ones and Photoshop does a better job of the B&W conversion than the scan driver software. In general leave most things to Photoshop like Image, Adjust, Shadow/Highlight [to bring out detail in shadows on scanned images from colour slide film), and Filter, Sharpen, UnSharp mask (sharpen image), as Photoshop offers far more control. Scanner hardware things like [Kodak] Digital ICE [remove scratches and dust on the film) have to be done by the scanner, but this is cosmetic and isn't designed to work with B&W film anyway (although if you scan in colour mode you can trick it into working, but 'results can vary' in B&W).
See detailed discussion of the cheap V750 and V700 film scanner flatbeds at http://www.photo-i.co.uk/Menus/reviews.htm
These scanners nominally scan at 6,400 dpi max and so produce excellent TEM scan 'working images' at 1200 dpi (in well under a minute) or you can scan smaller areas for enlargement up to 6400 dpi. You shouldn't need to do much post-processing, if any, as 1200 dpi. Use the film holders as distance from the glass platen is critical - check out 3rd party film holders as well. There are more very very expensive scanners that can resolve film better (well the film grain anyway), but these are typically £12,000 to £30,000+ (few, if any, 'cheaper' dedicated 35mm film scanners. E.g. Kodaks, can handle TEM film sizes greater than 9x6cm).
You do need Photoshop CS1 or greater (CS3 is the latest & recommended) as well though, but this isn't that expensive with educational discounts (£130).
Note that scanning at 6,400 dpi produces an enormous image file size [} 100Mb] in TIF format that will be highly prone to data corruption if archived. So keep that original negative as the archived master and don't rely on a digitised hi-res scanned image for long-term archiving. Passing through a second set of optics during scanning will always degrade the image anyway, and some detail will always be lost whatever the scanner used [compared to that seen in the original film negative/colour slide]. The amount of detail lost naturally depends on the scanner quality.
Any queries and just ask.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568
} ----- } Email: tomw-at-uidaho.edu } Name: Tom Williams } Organization: University of Idaho } Title-Subject: [Filtered] TEM negative to digital conversion } } Question: I have had a request from a user to convert several old TEM } negatives to a digital format. Normally I use a standard flat-bed } scanner for this which produces reasonable (although lower resolution) } negative and positive digital image conversion. The user in question } would like to try for a higher resolution image conversion than I can } provide. Does anyone have a suggested method for negative conversion } other than a flat-bed scanner? } } Thanks in advance. } } Thomas J. Williams, PhD } Center for Electron Microscopy and Microanalysis University of Idaho } Moscow, ID 83844-3025 } (208) 885-6785 } tomw-at-uidaho.edu
==============================Original Headers============================== 17, 21 -- From kjmorris-at-well.ox.ac.uk Mon Jul 14 07:49:33 2008 17, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6ECnWgK004360 17, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2008 07:49:33 -0500 17, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 17, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 17, 21 -- id 1KINUi-0003uM-2u 17, 21 -- for Microscopy-at-Microscopy.Com; Mon, 14 Jul 2008 13:49:32 +0100 17, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 17, 21 -- To: {Microscopy-at-Microscopy.Com} 17, 21 -- Subject: [Microscopy] viaWWW: TEM negative to digital conversion 17, 21 -- Date: Mon, 14 Jul 2008 13:49:31 +0100 17, 21 -- Message-ID: {FD4E36A3271943A6809B97F789EDAD6A-at-CytoWhizz} 17, 21 -- MIME-Version: 1.0 17, 21 -- Content-Type: text/plain; 17, 21 -- charset="iso-8859-1" 17, 21 -- X-Mailer: Microsoft Office Outlook 11 17, 21 -- thread-index: AcjlmP6vMrBjHRDYTQe0+oE65a5tFQAC664g 17, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 17, 21 -- Content-Transfer-Encoding: 8bit 17, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6ECnWgK004360 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 25 -- From Philip.Koeck-at-ki.se Mon Jul 14 08:10:15 2008 28, 25 -- Received: from smtp2.ki.se (smtp2.ki.se [130.237.98.101]) 28, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6EDAEQc018104 28, 25 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jul 2008 08:10:15 -0500 28, 25 -- Received: from smtp2.ki.se (smtp2.ki.se [127.0.0.1]) 28, 25 -- by localhost (Postfix) with SMTP id 3F7213DC0D6; 28, 25 -- Mon, 14 Jul 2008 15:10:14 +0200 (CEST) 28, 25 -- Received: from Rapana (rapana.csb.ki.se [130.237.109.49]) 28, 25 -- by smtp2.ki.se (Postfix) with ESMTP id 2C3363DC08D; 28, 25 -- Mon, 14 Jul 2008 15:10:14 +0200 (CEST) 28, 25 -- From: "Philip Koeck" {Philip.Koeck-at-ki.se} 28, 25 -- To: {kjmorris-at-well.ox.ac.uk} , {microscopy-at-microscopy.com} 28, 25 -- References: {200807141253.m6ECr93p008470-at-ns.microscopy.com} 28, 25 -- Subject: RE: [Microscopy] viaWWW: TEM negative to digital conversion 28, 25 -- Date: Mon, 14 Jul 2008 15:07:40 +0200 28, 25 -- Message-ID: {002101c8e5b2$98f854d0$316ded82-at-ad.biosci.ki.se} 28, 25 -- MIME-Version: 1.0 28, 25 -- Content-Type: text/plain; 28, 25 -- charset="iso-8859-1" 28, 25 -- X-Mailer: Microsoft Office Outlook 11 28, 25 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 28, 25 -- Thread-Index: AcjlsJI0S+yVLwdiSwuAl/WlZzdBaQAAPGdA 28, 25 -- In-Reply-To: {200807141253.m6ECr93p008470-at-ns.microscopy.com} 28, 25 -- Content-Transfer-Encoding: 8bit 28, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6EDAEQc018104 ==============================End of - Headers==============================
Sorry, I missed your posting, so did not respond. It the better late than never category: Yes, we use DSLR's rountinely here. We have looked at both Canon and Nikon cameras and have gone with Nikon's mainly because we had lenses and other parts already.
We have: D50, D80, D200, and D300.
Hands down the D300 is the best for Photomicroscopy work.
Why: (1) They move the "film" (imaging surface of the CMOS) back in the camera body just a tiny bit, but now it matches the film plane position of a 35mm camera - that means you can set parfocality with the eye-pieces! (2) You can On-screen focus the image - i.e. using the Nikon Camaera control software you can focus on the computer screen directly. (3) The D300 also has an HDMI output for display on any digital CRT. (4) You can use aperature prioity exposure - since the microscope does not have a camera controlable aperture its aperature is "fixed" and the camera can control only the shuttter speed. Seems simply enough, but all the Nikons below the D300 can not do this, they require a "real" nikon lens. I am not sure about the Canons. (5) We have run these cameras with very good results from GPF flouresence through normal BF imaging. (6) "Mirror-slap" is an issue on the D80, D50, and presumably D40. The cameras shake when taking a photo. Lighter scopelike a Nikno Optiphot will suffer from vibrations. Our Olympus AX-70 (Monster platform) suffers no shake. Your Zeiss Photomicroscope III is very beefy so it may not be an issue - but the you need to consider the long lever arm of the Phototube. The D200 and D300 have very very little mirror slap. The D300 has a mirror delay (mirror up, 2-seconds delay, shutter click) which deals with this although we have not ahd any problems anyway. (7) The negatives: The eye-piece and right angle viewer for the Nikon D-series cameras is horrible (The right-anlge viewer for the F4 bodies was fantastic). Your will need the right-angle viewer for anything less than the D300. Secondly, the Camera control software is not included with the camera and must be purchased separately.
I suggest you also check out www.DPReview.com and go to the user groups and ask questions there as well.
If you have any speific questions please ask. Hope this helps!
On 3 Jul 2008 at 17:37, vapatpxs-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'm back! } } We have a Zeiss Photomicroscope III and would like to buy a digital } camera for it. What are people's opinions? Would something like a } regular Canon or Nikon DSLR work? } } Let me know what works in you lab and what type of images are you } capturing mostly. I think ours will be used for paraffin work (H&E, } etc) and immunofluorescence. } } The microscope is beautiful with wonderful optics but the camera it } has on it is so old we can't update the drivers to match the computers } any more. } } Thanks in advance. } } Paula Sicurello } VA San Diego Healthcare System } Microscope Facility room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } } } } ==============================Original Headers============================== } 9, 24 -- From vapatpxs-at-yahoo.com Thu Jul 3 16:36:56 2008 } 9, 24 -- Received: from n59.bullet.mail.sp1.yahoo.com (n59.bullet.mail.sp1.yahoo.com [98.136.44.43]) } 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m63Latul025651 } 9, 24 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jul 2008 16:36:55 -0500 } 9, 24 -- Received: from [216.252.122.217] by n59.bullet.mail.sp1.yahoo.com with NNFMP; 03 Jul 2008 21:36:55 -0000 } 9, 24 -- Received: from [69.147.84.114] by t2.bullet.sp1.yahoo.com with NNFMP; 03 Jul 2008 21:36:55 -0000 } 9, 24 -- Received: from [127.0.0.1] by omp203.mail.sp1.yahoo.com with NNFMP; 03 Jul 2008 21:36:55 -0000 } 9, 24 -- X-Yahoo-Newman-Property: ymail-3 } 9, 24 -- X-Yahoo-Newman-Id: 340926.14865.bm-at-omp203.mail.sp1.yahoo.com } 9, 24 -- Received: (qmail 40820 invoked by uid 60001); 3 Jul 2008 21:36:55 -0000 } 9, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 9, 24 -- s=s1024; d=yahoo.com; } 9, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; } 9, 24 -- b=Op0AAg2DB8rOj1217SmnMcFQZ1XtxSm2FGeornIpBrhJw3szrOnEjc1sQ7Dj6/0fEusQiP2bNdq5/g3I72nGYpjD32SE0pe5Ve5SxmlUuju3hS6eVXV9ZXVUt6NTCyagRTzLGyCCDjiFgU7qgCEfmz2fzEtLis0ValdV0asqLoA=; } 9, 24 -- Received: from [132.239.85.200] by web46113.mail.sp1.yahoo.com via HTTP; Thu, 03 Jul 2008 14:36:55 PDT } 9, 24 -- X-Mailer: YahooMailWebService/0.7.199 } 9, 24 -- Date: Thu, 3 Jul 2008 14:36:55 -0700 (PDT) } 9, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} } 9, 24 -- Reply-To: vapatpxs-at-yahoo.com } 9, 24 -- Subject: Digital Camera } 9, 24 -- To: MSA BB {Microscopy-at-microscopy.com} } 9, 24 -- MIME-Version: 1.0 } 9, 24 -- Content-Type: text/plain; charset=us-ascii } 9, 24 -- Message-ID: {162024.40055.qm-at-web46113.mail.sp1.yahoo.com} } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 14, 25 -- From edelmare-at-muohio.edu Mon Jul 14 09:03:26 2008 14, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6EE3QLu032405 14, 25 -- for {microscopy-at-Microscopy.com} ; Mon, 14 Jul 2008 09:03:26 -0500 14, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 14, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m6EE3Qc5019064; 14, 25 -- Mon, 14 Jul 2008 10:03:26 -0400 14, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 14, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m6EE3PnM021051; 14, 25 -- Mon, 14 Jul 2008 10:03:26 -0400 14, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 14, 25 -- To: "vapatpxs-at-yahoo.com" {vapatpxs-at-yahoo.com} 14, 25 -- Date: Mon, 14 Jul 2008 10:03:25 -0400 14, 25 -- MIME-Version: 1.0 14, 25 -- Subject: Re: [Microscopy] Digital Camera 14, 25 -- CC: microscopy-at-Microscopy.com 14, 25 -- Message-ID: {487B246D.28389.DFF8C5A-at-edelmare.muohio.edu} 14, 25 -- Priority: normal 14, 25 -- In-reply-to: {200807032137.m63LbMXa026679-at-ns.microscopy.com} 14, 25 -- References: {200807032137.m63LbMXa026679-at-ns.microscopy.com} 14, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 14, 25 -- Content-type: text/plain; charset=US-ASCII 14, 25 -- Content-transfer-encoding: 7BIT 14, 25 -- Content-description: Mail message body 14, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Greetings all, The Microscopy and Microanalysis 2008 meeting count down has begun and the deadline for early registration is past.
There is still need of a few more student bursaries and/or volunteers to help with meeting activities such as room monitors for paper sessions, the internet cafe, and helping in the Volunteer and Mega Booth areas.
Students who participate in the bursary program are paid $10 for every hour worked to help offset meeting costs. Not only does working as a bursary benefit the society, it gives students a chance to interact with the established microscopy community. If you would like to participate in the bursary program, please check the *I wish to apply for a student bursary* box in section 2 of the registration form, or if already registered just let me know.
There is an added bonus of a $10 cash meal allotment for each morning or afternoon sessions worked for bursaries AND volunteers. Volunteers may also receive some compensation to help with meeting costs.
If you would like to help either as a bursary or volunteer, or if you have any questions, don't hesitate to contact me.
Thanks, Amanda Lawrence M&M 2008, Bursary co-coordinator
My experience may be a bit out of date. When I had the funds (about
4-5 years ago) we tested the then current Leica model against the Bal-Tec HPM 010 extensively - and bought the Bal-Tec. We got many more good freezes for a variety of sample types.
More recently, I have seen superb freezes achieved by the newer Leica model in the hands of others, so the choice may no longer be so clear cut?
Leica offers a lot more innovation with regard to sample holders, and depending on the course of your work, that consideration might be important. For using standard A + B hats, the Bal-Tec is still outstanding.
I said Kodak when I was thinking of the £2,000 Nikon Super CoolScan 9000 ED. Unfortunately I'm pretty sure it still can't scan above 9x6cm - too small for most TEM film and certainly it can't do A4 [21cm×29cm] film or A4 reflective scans.
It's also a bit expensive to be called 'budget', although there are cheaper Nikon 35mm film scanners [e.g. the £600 Coolscan V that only does 35mm film]. Ultimately for home & work use I chose a quality 'prosumer' A4 flatbed film/reflective scanner for price & flexibility as much as scan quality.
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568
-----Original Message----- X-from: Philip Koeck [mailto:Philip.Koeck-at-ki.se] Sent: 14 July 2008 14:08 To: kjmorris-at-well.ox.ac.uk; microscopy-at-microscopy.com
Hi Tom,
Obviously film scanners are the best way to digitise film, that’s why they are called that - it's the quality of the optics/mechanism that’s paramount for fast hi-res scanning though, coupled to how much you are prepared to pay, and there are lemons out there. You need a flatbed film scanner as you have a large negative size (dedicated 35mm film scanners not being much use unless you get some scissors). Rather than go through all the recent list-server chat from 2007ish on scanning TEM film [which you can find pretty easily I hope), I will send a copy of my recent article on 'scanning film on a tight budget' written for the Royal Microscopy Society's InFocus magazine.
Assuming you are scanning B&W film, scanner DMax isn't as important as scanner resolution (as most modern quality flatbed film scanners have a DMax in excess of that needed for B&W film, it's colour slide film that really needs that high DMax). Unfortunately manufacturer's lie about a scanners DMax and resolution differently so it is difficult to compare. However you can buy a decent flatbed A4 film scanner for under $800 (Epson V750 Pro) and $500 (Epson V700) - see the review link below. The Epson V750 is essentially the same scanner as the V700 but with improved scan optics lenses and other extras like better bundled software (both scan in either film transmission or reflective mode to A4 in size). There don't seem to be any flatbed film scanners to touch these for the [low] price.
Some say scanning in colour and converting to B&W is better [I scan colour slide/negatives mainly], this might be because all the scanners are colour detector ones and Photoshop does a better job of the B&W conversion than the scan driver software. In general leave most things to Photoshop like Image, Adjust, Shadow/Highlight [to bring out detail in shadows on scanned images from colour slide film), and Filter, Sharpen, UnSharp mask (sharpen image), as Photoshop offers far more control. Scanner hardware things like [Kodak] Digital ICE [remove scratches and dust on the film) have to be done by the scanner, but this is cosmetic and isn't designed to work with B&W film anyway (although if you scan in colour mode you can trick it into working, but 'results can vary' in B&W).
See detailed discussion of the cheap V750 and V700 film scanner flatbeds at http://www.photo-i.co.uk/Menus/reviews.htm
These scanners nominally scan at 6,400 dpi max and so produce excellent TEM scan 'working images' at 1200 dpi (in well under a minute) or you can scan smaller areas for enlargement up to 6400 dpi. You shouldn't need to do much post-processing, if any, as 1200 dpi. Use the film holders as distance from the glass platen is critical - check out 3rd party film holders as well. There are more very very expensive scanners that can resolve film better (well the film grain anyway), but these are typically £12,000 to £30,000+ (few, if any, 'cheaper' dedicated 35mm film scanners. E.g. Kodaks, can handle TEM film sizes greater than 9x6cm).
You do need Photoshop CS1 or greater (CS3 is the latest & recommended) as well though, but this isn't that expensive with educational discounts (£130).
Note that scanning at 6,400 dpi produces an enormous image file size [} 100Mb] in TIF format that will be highly prone to data corruption if archived. So keep that original negative as the archived master and don't rely on a digitised hi-res scanned image for long-term archiving. Passing through a second set of optics during scanning will always degrade the image anyway, and some detail will always be lost whatever the scanner used [compared to that seen in the original film negative/colour slide]. The amount of detail lost naturally depends on the scanner quality.
Any queries and just ask.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568
} ----- } Email: tomw-at-uidaho.edu } Name: Tom Williams } Organization: University of Idaho } Title-Subject: [Filtered] TEM negative to digital conversion } } Question: I have had a request from a user to convert several old TEM } negatives to a digital format. Normally I use a standard flat-bed } scanner for this which produces reasonable (although lower resolution) } negative and positive digital image conversion. The user in question } would like to try for a higher resolution image conversion than I can } provide. Does anyone have a suggested method for negative conversion } other than a flat-bed scanner? } } Thanks in advance. } } Thomas J. Williams, PhD } Center for Electron Microscopy and Microanalysis University of Idaho } Moscow, ID 83844-3025 } (208) 885-6785 } tomw-at-uidaho.edu
==============================Original Headers============================== 17, 21 -- From kjmorris-at-well.ox.ac.uk Mon Jul 14 07:49:33 2008 17, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6ECnWgK004360 17, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2008 07:49:33 -0500 17, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 17, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 17, 21 -- id 1KINUi-0003uM-2u 17, 21 -- for Microscopy-at-Microscopy.Com; Mon, 14 Jul 2008 13:49:32 +0100 17, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 17, 21 -- To: {Microscopy-at-Microscopy.Com} 17, 21 -- Subject: [Microscopy] viaWWW: TEM negative to digital conversion 17, 21 -- Date: Mon, 14 Jul 2008 13:49:31 +0100 17, 21 -- Message-ID: {FD4E36A3271943A6809B97F789EDAD6A-at-CytoWhizz} 17, 21 -- MIME-Version: 1.0 17, 21 -- Content-Type: text/plain; 17, 21 -- charset="iso-8859-1" 17, 21 -- X-Mailer: Microsoft Office Outlook 11 17, 21 -- thread-index: AcjlmP6vMrBjHRDYTQe0+oE65a5tFQAC664g 17, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 17, 21 -- Content-Transfer-Encoding: 8bit 17, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6ECnWgK004360 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jul 15 04:55:39 2008 38, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 38, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6F9tceh003668 38, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 15 Jul 2008 04:55:39 -0500 38, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 38, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 38, 23 -- id 1KIhFy-0003bI-9r 38, 23 -- for Microscopy-at-Microscopy.Com; Tue, 15 Jul 2008 10:55:38 +0100 38, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 38, 23 -- To: {Microscopy-at-Microscopy.Com} 38, 23 -- References: {200807141253.m6ECr93p008470-at-ns.microscopy.com} {002101c8e5b2$98f854d0$316ded82-at-ad.biosci.ki.se} 38, 23 -- Subject: RE: [Microscopy] viaWWW: TEM negative to digital conversion 38, 23 -- Date: Tue, 15 Jul 2008 10:55:38 +0100 38, 23 -- Message-ID: {A13E717BFC344C5AAF14902829CA9DCF-at-CytoWhizz} 38, 23 -- MIME-Version: 1.0 38, 23 -- Content-Type: text/plain; 38, 23 -- charset="iso-8859-1" 38, 23 -- X-Mailer: Microsoft Office Outlook 11 38, 23 -- thread-index: AcjlsJI0S+yVLwdiSwuAl/WlZzdBaQAAPGdAACr78CA= 38, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 38, 23 -- In-Reply-To: {002101c8e5b2$98f854d0$316ded82-at-ad.biosci.ki.se} 38, 23 -- Content-Transfer-Encoding: 8bit 38, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6F9tceh003668 ==============================End of - Headers==============================
We do have the Wohlwend HPF (sold by TechnoTrade in the USA) and have had it for two years. We regularly get very good freezing and have had no maintenance issues. It is easy to set up and I have graduate students using it without supervision after a short training session. They have much more trouble getting their specimens in the planchettes than the freezing itself which is very easy. Once they work out their loading technique than it goes very quickly.
We usually freeze about 6 planchettes per sample and 4-5 samples in a run. This takes about 50L of nitrogen from initial cool-down to completion. We rarely have to thin section more than 1-2 samples to find well-frozen tissue.
We do a lot of cultured mammalian cells, cyanobacteria, and plant material. Problems we have had are not related to the freezing but rather to infiltration of some of the more difficult samples. For example, we were not able to get good infiltration of whole drosophila eyes in HM20 resin processed at -50oC on our first try...have to work on that one. Any suggestions?
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
} From: {a.d.mckinnon-at-abdn.ac.uk} } Reply-To: {a.d.mckinnon-at-abdn.ac.uk} } Date: Tue, 15 Jul 2008 03:08:54 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] RE: Listserver HPF responses } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Many thanks to all who responded. The appraisals have been extremely useful } and much appreciated. } } As it appears that the replies have not been distributed to the list server } and following a request for info, here they are: } } Alastair } } Alastair McKinnon } IMS Histology & EM Facility Manager, R2.26 } University of Aberdeen, Institute of Medical Science } Foresterhill, Aberdeen, AB25 2ZD } tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) } fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk } www.abdn.ac.uk/ims/h-em } } ------------------------------------------------------------------------------ } --------------- } } My experience may be a bit out of date. When I had the funds (about } } 4-5 years ago) we tested the then current Leica model against the Bal-Tec HPM } 010 extensively - and bought the Bal-Tec. We got many more good freezes for a } variety of sample types. } } More recently, I have seen superb freezes achieved by the newer Leica model in } the hands of others, so the choice may no longer be so clear cut? } } Leica offers a lot more innovation with regard to sample holders, and } depending on the course of your work, that consideration might be important. } For using standard A + B hats, the Bal-Tec is still outstanding. } } Good luck. } } David Hall } } ------------------------------------------------------------------------------ } ----------------- } } Subject: Re: Bal-Tec HPM100 } } Dear Alastair, } } sorry for my late reply, I was out of town. Our Bal-Tec HPM100 was installed } like four weeks ago and so far I didn't find the time to really use it (aside } from a few test runs). But I will start soon to seriously use the machine and } will in any case keep you updated on my experience. } } About the factors that influenced my decision to go for the HPM100: } } Initially I had to make a decision between the Leica EM PACT 2 (with RTS; } rapid transfer system) and the old Bal-Tec HPM010, as at the time when I was } on "high pressure freezer shopping tour" the Bal-Tec HPM100 was not on the } market. I talked to a lot of people, users of the Leica EM PACT and EM PACT 2 } and users of the Bal-Tec HPM010. By talking to all these people I got the } impression that when it comes to hard to freeze samples the HPM010 can give } you better results than the Leica EM PACT or EM PACT 2. So at one point I } decided to go for the HPM010. The only real disadvantage I saw with that } machine was that - compared to the Leica machines - it is really kinda } complicated to operate and needs a lot of liquid nitrogen. But I was ready and } prepared to live with that. } } Then Bal-Tec came up with the new HPM100 and I was really happy about that new } machine because I think (or maybe lets better say hope) that it combines the } pros of the old HPM010 (good freezing results with a wide variety of } biological samples) with the pros of the Leica machine (easy to use, low } consumption of liquid nitrogen). One of the things I also really like about } the HPM100 is that the size and shape of the sample holders can be modified. } So besides the standard 2 mm sample holders there will be 5 mm sample holders } available, in my eyes a real plus. } } I am working with all kinds of samples: yeast and Chlamydomonas at the moment } beeing the most important ones, but also Drosophila, Caenorhabditis, higher } plants, mammalian tissue samples etc. Rapid transfer for live cell co-relative } study was not a factor in my purchase decision, as with the HPM010 a rapid } transfer would have been really hard to realise. With the new HPM100 a rapid } transfer for live cell co-relative study should be absolutely doable, and as } far as I know the are developing such a system for the HPM100. } } One thing which concerns me a little is how the situation will develop now } after Bal-Tec got sold to Leica. So will Leica continue both machines, or did } they just buy Bal-Tec to get rid of a competitor and are there plans to cease } the production of the HPM100. I am having no idea, maybe you can get some } honest informations regarding this from your Leica representative. } } I hope this helps a little. There would be way more to say but I am in a hurry } (a lot of travelling at the moment). I will be out of town next week but back } in the lab on July 21st. So if you have any further question don't hesitate to } contact me, I will be happy to help if I can. } } As I wrote above, when I had to make the decision I talked to a lot of people } and I found it extremely helpful to get all these comments and feedback from } people actually using the machines. } } All the best, } } Stefan } } } } ------------------------------------------------------------------------------ } ----------------- } } Hi Alistair, } } We currently have two high pressure freezers at our facility; a Wohlwend } Compact 01 and the previous model Baltec HPM 010. My boss is going to make me } sell one of them. I have not decided which, they both work very well. If you } are interested purchasing used feel free to request more information. } } Cheers, } } KD Derr } } ------------------------------------------------------------------------------ } ------------------ } } Hi Alastair, } } I have what I believe to be the oldest functioning HPM010 in the world. } [22yrs, it's used fairly infrequently] It's my experience that the machine is } built to withstand all the abuse one can imagine. As long as the seals are } regularly changed and the rest of the maintenance is kept up, our machine } works great every time. } } The design has changed very little over the years, mostly new bells and } whistles. I haven't had experience with the newest Leica offering, but was } disappointed when I initially tested the first freezer they marketed. } } Cheers, } Jay } } } ------------------------------------------------------------------------------ } ------- } } Alastair, } } There is another and better alternative: } } http://www.technotradeinc.com/Wohlwend/hpf%20default.htm } {http://www.technotradeinc.com/Wohlwend/hpf%20default.htm} } } This is the US distributor, but they can tell you who deals with the UK, I'm } sure. } } This is the guy who invented and developed the Baltec HPF 010. They split, and } Wohlwend set up independently. } } I used the HPF 010 at a previous position, and consider it *much* better fhan } the Leice Empact in all respects. The current version that Wohlwend is making } is an improved version of the HPF 010. } } Debby Sherman at Purdue has one of these, and as I understand likes it very } much. } } I just wish we could get one here. } } Phil } } ----------------------------------------------------------------------------- } } Hello, } } I would recommend you to also consider the Wohlwend HPF Compact 001 High } pressure freezing machine. I believe that it is better than the competing } machines. I have it in use for more than 5 years and it works well and } provides great results, especially for adherent cultivated cells. } } The suppliers email is: } martin-wohlwend-at-bluewin.ch } } With kind regards Paul Walther } } Here are some of our recent publications with the machine: } } Selection of book articles and peer reviewed papers showing results obtained } with the Wohlwend HPM Compact 01 } } Walther P. (2008) High Resolution Cryoscanning Electron Microscopy of } Biological Samples. Book article in: Schatten H, Pawley J. B. (eds) Biological } Low-Voltage Scanning Electron Microscopy. Springer New York. ISBN } 978-0-387-72970-1 (Print) 978-0-387.72972-5 (Online). pp 245-261. Summary } {http://www.springerlink.com/content/g53un05383l44270/} } } Schlottmann S, Buback F, Stahl B, Meierhenrich R, Walter P, Georgieff M, } Senftleben U. (2008) Prolonged Classical NF-κB Activation Prevents Autophagy } upon E. coli Stimulation In Vitro: A Potential Resolving Mechanism of } Inflammation. Mediators of Inflamation doi:10.1155/2008/725854 (in press) } } Barbosa-Barros L, de la Maza A, Estelrich J, Linares AM, Feliz M, Walther P, } Pons R, LÓpez O. (2008) Penetration and Growth of DPPC/DHPC Bicelles Inside } the Stratum Corneum of the Skin. Langmuir. 24, 5700-5706 Summary } {http://www.ncbi.nlm.nih.gov/pubmed/18471002?ordinalpos=1&itool=EntrezSystem2. } PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum} } } Hölzle A, Fischer S, Heyer R, SchÃπtz S, Zacharias M, Walther P, Allers T, } Marchfelder A. (2008). Maturation of the 5S rRNA 5' end is catalyzed in vitro } by the endonuclease tRNase Z in the archaeon H. volcanii. RNA. 2008 14, } 928-937 Summary } {http://www.ncbi.nlm.nih.gov/pubmed/18369184?ordinalpos=1&itool=EntrezSystem2. } PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum} } } Buser C, Walther P. (2008) Freeze-Substitution: The Addition of Water to Polar } Solvents Enhances the Retention of Structure and Acts at Temperatures Around } â•„60 °C. J. Microsc. 230, 268-77. Summary } {http://www.ncbi.nlm.nih.gov/pubmed/18445157?ordinalpos=1&itool=EntrezSystem2. } PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum} } } Rupp B, Ruzsics Z, Buser C, Adler B, Walther P, Koszinowski UH. (2007) A } random screen for dominant-negative mutants of the cytomegalovirus nuclear } egress protein M50. J Virol. 81, 5508-5517 Summary } {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abs } tractPlus&list_uids=17376929&query_hl=1&itool=pubmed_docsum} } } Buser C, Walther P, Mertens T, Michel D. (2007) Cytomegalovirus primary } envelopment occurs at large infoldings of the inner nuclear membrane. } J Virol. 81, 3042-8. Summary } {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abs } tractPlus&list_uids=17192309&query_hl=1&itool=pubmed_docsum} } } } } Buser C, Fleischer F, Mertens T, Michel D, Schmidt V, Walther P. (2007) } Quantitative investigation of murine cytomegalovirus nucleocapsid interaction. } J. Mircrosc. 228, 78-87. Summary } {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2818.2007.01825.x} } } Martin R, Hild S, Walther P, Ploss K, Boland W, Tomaschko K.-H. (2007) } Granular Chitin in the Epidermis of Nudibranch Molluscs. Biol. Bull. 213, } 307-315. Summary } {http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToS } earch=18083970&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP } anel.Pubmed_RVDocSum} } } Wild P, Ziegler U, Schraner EM, Senn C, Tobler K, Engels M, Loepfe E, } Ackermann M, Mueller M, Walther P. (2004) Impairment of Nuclear Pores in } Bovine Herpesvirus 1 Infected MDBK Cells. J. Virol. 79, 1071-1083. } } Nijsse J, Walther P and Hoekstra FA. (2004) Cold-induced imbibition damage of } lettuce embryos: A study using cryo-scanning electron microscopy. Seed Science } Research, 14, 117-126. } } -------------------------------------------------------- } } you may also have a look to the Wohlwend HPF machine which to my knowledge and } experience is absolutely equivalent if not better in all major aspects. } } If interested, please contact Paul Walther at Ulm University for further } details on this machine. } } paul.walther-at-uni-ulm.de } } best regards, } } Reinhard Rachel } } -- } } ---------------------- } } ------------------------------------------------------------------------------ } ----------------- } } Hi Alasdair, } } I just purchased an EMPACT2 from Leica and it is an amazingly simple machine } to use. It is also very reliable for reproducibility f freezing. } } As you pointed out, sometimes it is good to have a larger specimen planchet, } but by giving this up you end up with a machine that can produce more } well-frozen specimens than the Baltec machine. I have used both machines over } the last three years and chose the EMPACT2 for its ease of use and } reproducibility. I was never allowed to use the Baltec machine alone - only } with an "expert" present (Kent McDonald in Berkeley or Heinz Schwarz in } Tuebingen). Each time it worked well. } } However when I was introduced to the EMPACT2, it was a case of someone showing } me how to load the specimen and then how to put this into the machine, and I } was off on my own. Since then I purchased my own machine and have had a great } success with it. I even teach high-school students how to prepare specimens } with it. } } Bear in mind that historically Leica will buy up their competition, swear that } they will continue to support the machines and then quietly phase them out } (where are the LKB ultramicrotomes these days?). } } If you really are looking for the Baltec-style machine then you should see if } the UK has a distributor for the Wohlwend HPF. Wohlwend machines are built on } the same principle as the Baltec units and from what I hear they are very } reliable. I am pretty sure that Paul Walther in Ulm is still using one of the } first ones ever built. Maybe you should contact him for a reference. } } Good luck with your search. } } Regards, } } Paul Webster. } } } } The University of Aberdeen is a charity registered in Scotland, No SC013683. } } } ==============================Original Headers============================== } 89, 30 -- From a.d.mckinnon-at-abdn.ac.uk Tue Jul 15 03:06:03 2008 } 89, 30 -- Received: from mailhub2.abdn.ac.uk (mailhub2.abdn.ac.uk } [139.133.7.24]) } 89, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m6F863Hx005981 } 89, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jul 2008 03:06:03 -0500 } 89, 30 -- Received: from uo-mail-3.uoa.abdn.ac.uk ([139.133.15.93] } helo=mail.abdn.ac.uk) } 89, 30 -- by mailhub2.abdn.ac.uk with esmtp (Exim 4.52) } 89, 30 -- id 1KIfXu-0007C2-K2 } 89, 30 -- for Microscopy-at-microscopy.com; Tue, 15 Jul 2008 09:06:02 +0100 } 89, 30 -- Received: from VMAILB.uoa.abdn.ac.uk ([139.133.15.92]) by } 89, 30 -- uo-mail-3.uoa.abdn.ac.uk ([139.133.15.93]) with mapi; Tue, 15 Jul } 2008 } 89, 30 -- 09:06:02 +0100 } 89, 30 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} } 89, 30 -- To: "'Microscopy-at-microscopy. com (Microscopy-at-microscopy.com)'" } 89, 30 -- {Microscopy-at-microscopy.com} } 89, 30 -- Date: Tue, 15 Jul 2008 09:06:02 +0100 } 89, 30 -- Subject: RE: Listserver HPF responses } 89, 30 -- Thread-Topic: Listserver HPF responses } 89, 30 -- Thread-Index: AcjeZDseSPG9DDbXT4GLPTs02KCJBQDQkt7gASo4rwAAAHSOcA== } 89, 30 -- Message-ID: } {A3317CA649BC8C4998EED327D66A8D9528123654B0-at-VMAILB.uoa.abdn.ac.uk} } 89, 30 -- References: } {A3317CA649BC8C4998EED327D66A8D9528123654AF-at-VMAILB.uoa.abdn.ac.uk} } 89, 30 -- In-Reply-To: } {A3317CA649BC8C4998EED327D66A8D9528123654AF-at-VMAILB.uoa.abdn.ac.uk} } 89, 30 -- Accept-Language: en-US } 89, 30 -- Content-Language: en-US } 89, 30 -- X-MS-Has-Attach: } 89, 30 -- X-MS-TNEF-Correlator: } 89, 30 -- acceptlanguage: en-US } 89, 30 -- Content-Type: text/plain; charset="utf-8" } 89, 30 -- MIME-Version: 1.0 } 89, 30 -- Content-Transfer-Encoding: 8bit } 89, 30 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id } m6F863Hx005981 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 32 -- From dsherman-at-purdue.edu Tue Jul 15 07:24:58 2008 8, 32 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6FCOvxq021310 8, 32 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2008 07:24:57 -0500 8, 32 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 8, 32 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m6FCOpcJ026685; 8, 32 -- Tue, 15 Jul 2008 08:24:51 -0400 8, 32 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 8, 32 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m6FCOnRl011097; 8, 32 -- Tue, 15 Jul 2008 08:24:51 -0400 8, 32 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 32 -- Tue, 15 Jul 2008 08:24:50 -0400 8, 32 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 32 -- Tue, 15 Jul 2008 12:20:30 +0000 8, 32 -- User-Agent: Microsoft-Entourage/12.11.0.080522 8, 32 -- Date: Tue, 15 Jul 2008 08:20:28 -0400 8, 32 -- Subject: Re: [Microscopy] RE: Listserver HPF responses 8, 32 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 32 -- To: {a.d.mckinnon-at-abdn.ac.uk} , 8, 32 -- "message to: MSA list" {microscopy-at-microscopy.com} 8, 32 -- Message-ID: {C4A20E4C.3008C%dsherman-at-purdue.edu} 8, 32 -- Thread-Topic: [Microscopy] RE: Listserver HPF responses 8, 32 -- Thread-Index: AcjmdSqUPtr5jqeoQ36ucZpyOd0Kbg== 8, 32 -- In-Reply-To: {200807150808.m6F88s5e009311-at-ns.microscopy.com} 8, 32 -- Mime-version: 1.0 8, 32 -- Content-type: text/plain; 8, 32 -- charset="UTF-8" 8, 32 -- X-OriginalArrivalTime: 15 Jul 2008 12:24:50.0334 (UTC) FILETIME=[C6F18BE0:01C8E675] 8, 32 -- X-PMX-Version: 5.4.0.320885 8, 32 -- X-PerlMx-Virus-Scanned: Yes 8, 32 -- Content-Transfer-Encoding: 8bit 8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6FCOvxq021310 ==============================End of - Headers==============================
The problem with the Nikon scanner, and my Minolta Dimage Scan Multi Pro, is that they will not accept films larger than 6x9 cm. This is why I use a digital camera and a lightbox to copy my EM negatives. The resolution is fine for study "prints". For publication, find a professional photo lab to scan your negatives. The Microtek ArtixScan M1 Pro, US$699 street price, does both prints to 8.5x14 inches and will scan films up to 4x5 in a separate chamber. This scanner is popular with professional photographers, the manufacturer claims 4800 dpi and 48 bit depth, includes SilverFast AI software.
Geoff
kjmorris-at-well.ox.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } I said Kodak when I was thinking of the £2,000 Nikon Super CoolScan 9000 ED. } Unfortunately I'm pretty sure it still can't scan above 9x6cm - too small } for most TEM film and certainly it can't do A4 [21cm×29cm] film or A4 } reflective scans. } } It's also a bit expensive to be called 'budget', although there are cheaper } Nikon 35mm film scanners [e.g. the £600 Coolscan V that only does 35mm } film]. Ultimately for home & work use I chose a quality 'prosumer' A4 } flatbed film/reflective scanner for price & flexibility as much as scan } quality. } } Keith } } } --------------------------------------------------------------------------- } Dr Keith J. Morris } Molecular Cytogenetics and Microscopy Core } Laboratory 00/069 and 00/070 } The Wellcome Trust Centre for Human Genetics } Roosevelt Drive } Oxford } OX3 7BN } United Kingdom } Telephone: +44 (0)1865 287568 } } Email: kjmorris-at-well.ox.ac.uk } Web-pages: } http://www.well.ox.ac.uk/cytogenetics/ } } } } -----Original Message----- } X-from: Philip Koeck [mailto:Philip.Koeck-at-ki.se] } Sent: 14 July 2008 14:08 } To: kjmorris-at-well.ox.ac.uk; microscopy-at-microscopy.com } Subject: RE: [Microscopy] viaWWW: TEM negative to digital conversion } } Hi, } } if you are going to buy a high-resolution scanner you should definitely } consider the Nikon 9000, which, according to reliable sources, outperforms } the top of the line models from all competitors in the same price-range by } far. } } Philip } } -----Original Message----- } X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk] } Sent: 14 July 2008 14:53 } To: philip.koeck-at-biosci.ki.se } Subject: [Microscopy] viaWWW: TEM negative to digital conversion } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Tom, } } Obviously film scanners are the best way to digitise film, that’s why they } are called that - it's the quality of the optics/mechanism that’s paramount } for fast hi-res scanning though, coupled to how much you are prepared to } pay, and there are lemons out there. You need a flatbed film scanner as you } have a large negative size (dedicated 35mm film scanners not being much use } unless you get some scissors). Rather than go through all the recent } list-server chat from 2007ish on scanning TEM film [which you can find } pretty easily I hope), I will send a copy of my recent article on 'scanning } film on a tight budget' written for the Royal Microscopy Society's InFocus } magazine. } } Assuming you are scanning B&W film, scanner DMax isn't as important as } scanner resolution (as most modern quality flatbed film scanners have a DMax } in excess of that needed for B&W film, it's colour slide film that really } needs that high DMax). Unfortunately manufacturer's lie about a scanners } DMax and resolution differently so it is difficult to compare. However you } can buy a decent flatbed A4 film scanner for under $800 (Epson V750 Pro) and } $500 (Epson V700) - see the review link below. The Epson V750 is essentially } the same scanner as the V700 but with improved scan optics lenses and other } extras like better bundled software (both scan in either film transmission } or reflective mode to A4 in size). There don't seem to be any flatbed film } scanners to touch these for the [low] price. } } Some say scanning in colour and converting to B&W is better [I scan colour } slide/negatives mainly], this might be because all the scanners are colour } detector ones and Photoshop does a better job of the B&W conversion than the } scan driver software. In general leave most things to Photoshop like Image, } Adjust, Shadow/Highlight [to bring out detail in shadows on scanned images } from colour slide film), and Filter, Sharpen, UnSharp mask (sharpen image), } as Photoshop offers far more control. Scanner hardware things like [Kodak] } Digital ICE [remove scratches and dust on the film) have to be done by the } scanner, but this is cosmetic and isn't designed to work with B&W film } anyway (although if you scan in colour mode you can trick it into working, } but 'results can vary' in B&W). } } See detailed discussion of the cheap V750 and V700 film scanner flatbeds at } http://www.photo-i.co.uk/Menus/reviews.htm } } These scanners nominally scan at 6,400 dpi max and so produce excellent TEM } scan 'working images' at 1200 dpi (in well under a minute) or you can scan } smaller areas for enlargement up to 6400 dpi. You shouldn't need to do much } post-processing, if any, as 1200 dpi. Use the film holders as distance from } the glass platen is critical - check out 3rd party film holders as well. } There are more very very expensive scanners that can resolve film better } (well the film grain anyway), but these are typically £12,000 to £30,000+ } (few, if any, 'cheaper' dedicated 35mm film scanners. E.g. Kodaks, can } handle TEM film sizes greater than 9x6cm). } } You do need Photoshop CS1 or greater (CS3 is the latest & recommended) as } well though, but this isn't that expensive with educational discounts } (£130). } } Note that scanning at 6,400 dpi produces an enormous image file size } [} 100Mb] in TIF format that will be highly prone to data corruption if } archived. So keep that original negative as the archived master and don't } rely on a digitised hi-res scanned image for long-term archiving. Passing } through a second set of optics during scanning will always degrade the image } anyway, and some detail will always be lost whatever the scanner used } [compared to that seen in the original film negative/colour slide]. The } amount of detail lost naturally depends on the scanner quality. } } Any queries and just ask. } } Regards } } Keith } } --------------------------------------------------------------------------- } Dr Keith J. Morris } Molecular Cytogenetics and Microscopy Core } Laboratory 00/069 and 00/070 } The Wellcome Trust Centre for Human Genetics } Roosevelt Drive } Oxford } OX3 7BN } United Kingdom } Telephone: +44 (0)1865 287568 } } Email: kjmorris-at-well.ox.ac.uk } Web-pages: } http://www.well.ox.ac.uk/cytogenetics/ } } } } } ----- } } Email: tomw-at-uidaho.edu } } Name: Tom Williams } } Organization: University of Idaho } } Title-Subject: [Filtered] TEM negative to digital conversion } } } } Question: I have had a request from a user to convert several old TEM } } negatives to a digital format. Normally I use a standard flat-bed } } scanner for this which produces reasonable (although lower resolution) } } negative and positive digital image conversion. The user in question } } would like to try for a higher resolution image conversion than I can } } provide. Does anyone have a suggested method for negative conversion } } other than a flat-bed scanner? } } } } Thanks in advance. } } } } Thomas J. Williams, PhD } } Center for Electron Microscopy and Microanalysis University of Idaho } } Moscow, ID 83844-3025 } } (208) 885-6785 } } tomw-at-uidaho.edu } } } } } } } ==============================Original Headers============================== } 17, 21 -- From kjmorris-at-well.ox.ac.uk Mon Jul 14 07:49:33 2008 } 17, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m6ECnWgK004360 } 17, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 14 Jul 2008 07:49:33 } -0500 } 17, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 17, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 17, 21 -- id 1KINUi-0003uM-2u } 17, 21 -- for Microscopy-at-Microscopy.Com; Mon, 14 Jul 2008 13:49:32 } +0100 } 17, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 17, 21 -- To: {Microscopy-at-Microscopy.Com} } 17, 21 -- Subject: [Microscopy] viaWWW: TEM negative to digital conversion } 17, 21 -- Date: Mon, 14 Jul 2008 13:49:31 +0100 } 17, 21 -- Message-ID: {FD4E36A3271943A6809B97F789EDAD6A-at-CytoWhizz} } 17, 21 -- MIME-Version: 1.0 } 17, 21 -- Content-Type: text/plain; } 17, 21 -- charset="iso-8859-1" } 17, 21 -- X-Mailer: Microsoft Office Outlook 11 } 17, 21 -- thread-index: AcjlmP6vMrBjHRDYTQe0+oE65a5tFQAC664g } 17, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 } 17, 21 -- Content-Transfer-Encoding: 8bit } 17, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m6ECnWgK004360 } ==============================End of - Headers============================== } } } } } } ==============================Original Headers============================== } 38, 23 -- From kjmorris-at-well.ox.ac.uk Tue Jul 15 04:55:39 2008 } 38, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) } 38, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6F9tceh003668 } 38, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 15 Jul 2008 04:55:39 -0500 } 38, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) } 38, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 38, 23 -- id 1KIhFy-0003bI-9r } 38, 23 -- for Microscopy-at-Microscopy.Com; Tue, 15 Jul 2008 10:55:38 +0100 } 38, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 38, 23 -- To: {Microscopy-at-Microscopy.Com} } 38, 23 -- References: {200807141253.m6ECr93p008470-at-ns.microscopy.com} {002101c8e5b2$98f854d0$316ded82-at-ad.biosci.ki.se} } 38, 23 -- Subject: RE: [Microscopy] viaWWW: TEM negative to digital conversion } 38, 23 -- Date: Tue, 15 Jul 2008 10:55:38 +0100 } 38, 23 -- Message-ID: {A13E717BFC344C5AAF14902829CA9DCF-at-CytoWhizz} } 38, 23 -- MIME-Version: 1.0 } 38, 23 -- Content-Type: text/plain; } 38, 23 -- charset="iso-8859-1" } 38, 23 -- X-Mailer: Microsoft Office Outlook 11 } 38, 23 -- thread-index: AcjlsJI0S+yVLwdiSwuAl/WlZzdBaQAAPGdAACr78CA= } 38, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 } 38, 23 -- In-Reply-To: {002101c8e5b2$98f854d0$316ded82-at-ad.biosci.ki.se} } 38, 23 -- Content-Transfer-Encoding: 8bit } 38, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6F9tceh003668 } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 11, 29 -- From mcauliff-at-umdnj.edu Tue Jul 15 09:40:22 2008 11, 29 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6FEeLaH020435 11, 29 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2008 09:40:21 -0500 11, 29 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 11, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 7788F4BE7C 11, 29 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2008 10:40:19 -0400 (EDT) 11, 29 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 11, 29 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 12D93A3B7C 11, 29 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2008 10:40:18 -0400 (EDT) 11, 29 -- Received: from ([10.32.15.171]) 11, 29 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.156054538; 11, 29 -- Tue, 15 Jul 2008 10:40:01 -0400 11, 29 -- Received: from [127.0.0.1] ([10.32.15.102]) 11, 29 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 11, 29 -- Oct 12 2007; 32bit)) with ESMTP id {0K4100EYYY2PPME0-at-umduwc02.umdnj.edu} for 11, 29 -- microscopy-at-microscopy.com; Tue, 15 Jul 2008 10:40:01 -0400 (EDT) 11, 29 -- Date: Tue, 15 Jul 2008 10:40:48 -0400 11, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 11, 29 -- Subject: Re: [Microscopy] RE: viaWWW: TEM negative to digital conversion 11, 29 -- In-reply-to: {200807150956.m6F9ubtl005176-at-ns.microscopy.com} 11, 29 -- To: kjmorris-at-well.ox.ac.uk, microscopy-at-microscopy.com 11, 29 -- Message-id: {487CB6F0.70206-at-umdnj.edu} 11, 29 -- MIME-version: 1.0 11, 29 -- Content-type: text/plain; charset=windows-1252; format=flowed 11, 29 -- References: {200807150956.m6F9ubtl005176-at-ns.microscopy.com} 11, 29 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id m6FEeLaH020435 ==============================End of - Headers==============================
A workshop on Aberration Correction for postgraduate students and post- doctoral scientists will be held on 26-27 November 2008 in Eynsham Hall, near Oxford, UK. It is sponsored by the European ESTEEM network but some places are available for participants from outside Europe. The workshop will cover the background and basic theory of optics and image formation in TEM and STEM, and the direct and indirect correction of lens aberrations. See http://www-em.materials.ox.ac.uk/esteem/abcorrworkshop.html
The workshop follows directly on from a Royal Society Discussion Meeting on "New Possibilities with Aberration Corrected Electron Microscopy" held in London on 24-25 November 2008. For this Discussion Meeting see http://royalsociety.org/event.asp?id=6073&month=11,2008
Crispin Hetherington Dept Materials Oxford University
==============================Original Headers============================== 6, 25 -- From crispin.hetherington-at-materials.ox.ac.uk Tue Jul 15 10:10:39 2008 6, 25 -- Received: from relay7.mail.ox.ac.uk (relay7.mail.ox.ac.uk [129.67.1.167]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6FFAdlX003838 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Tue, 15 Jul 2008 10:10:39 -0500 6, 25 -- Received: from smtp1.mail.ox.ac.uk ([129.67.1.207]) 6, 25 -- by relay7.mail.ox.ac.uk with esmtp (Exim 4.69) 6, 25 -- (envelope-from {crispin.hetherington-at-materials.ox.ac.uk} ) 6, 25 -- id 1KImAo-0001js-NT 6, 25 -- for Microscopy-at-Microscopy.Com; Tue, 15 Jul 2008 16:10:38 +0100 6, 25 -- Received: from crispin-macmini.materials.ox.ac.uk ([129.67.84.177]) 6, 25 -- by smtp1.mail.ox.ac.uk with esmtpsa (TLSv1:AES128-SHA:128) 6, 25 -- (Exim 4.69) 6, 25 -- (envelope-from {crispin.hetherington-at-materials.ox.ac.uk} ) 6, 25 -- id 1KImAo-00077Y-3t 6, 25 -- for Microscopy-at-Microscopy.Com; Tue, 15 Jul 2008 16:10:38 +0100 6, 25 -- Message-Id: {1BD88605-BEB0-4F4D-8F63-FAEA3BA2BC64-at-materials.ox.ac.uk} 6, 25 -- From: Crispin Hetherington {crispin.hetherington-at-materials.ox.ac.uk} 6, 25 -- To: Microscopy-at-Microscopy.Com 6, 25 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 25 -- Content-Transfer-Encoding: 7bit 6, 25 -- Mime-Version: 1.0 (Apple Message framework v926) 6, 25 -- Subject: TEM: UK meetings on Aberration Correction in November 2008 6, 25 -- Date: Tue, 15 Jul 2008 16:10:37 +0100 6, 25 -- X-Mailer: Apple Mail (2.926) 6, 25 -- X-Oxford-Username: crispin ==============================End of - Headers==============================
The Biotron Centre for Experimental Climate Change Research at the University of Western Ontario in London ON Canada, has an opening for a full time, full benefits Microscopy Imaging Technologist. Our Imaging Module offers full service Microscopic Imaging facilities - FEI CM 10 TEM with digital camera, Philips 420 TEM with EDS, Hitachi S3400-N VP with EDS, Zeiss LSM Live 5 DOU Confocal (Vario 2), Zeiss Z1 5 channel fluorescence fully digital microscope and digital imaging workstation, Zeiss Axioskop II Mot 3 channel fluorescence with digital imaging workstation, Zeiss Lumar V12 fully digital Stereomicroscope with 3 channel fluorescence and digital imaging workstation, Quartz/PCI WAM image database, 10 Sun imaging workstations, Riechert/LKB ultramicrotomes and a full service prep lab including cell culture facilities.
We are looking for a microscopist experienced in confocal and/or scanning electron microscopies - to view the job description and apply please see the UWO Human resources Web page here: www.uwo.ca/humanresources/applicant/careers_index2.htm Click on "I am Exploring a Career at Western" then "view Career Opportunities" then "Technical (Non Teaching Staff)
Thanks Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
==============================Original Headers============================== 8, 26 -- From rjharris-at-uwo.ca Tue Jul 15 11:00:31 2008 8, 26 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6FG0VDT018642 8, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Jul 2008 11:00:31 -0500 8, 26 -- MIME-version: 1.0 8, 26 -- Content-type: text/plain; charset=iso-8859-1 8, 26 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 8, 26 -- by zeppo.mail.uwo.pri 8, 26 -- (Sun Java(tm) System Messaging Server 6.3-5.02 (built Oct 12 2007; 32bit)) 8, 26 -- with ESMTP id {0K420017I1SCM0K0-at-zeppo.mail.uwo.pri} for 8, 26 -- microscopy-at-microscopy.com; Tue, 15 Jul 2008 12:00:13 -0400 (EDT) 8, 26 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 8, 26 -- by zeppo.mail.uwo.pri 8, 26 -- (Sun Java(tm) System Messaging Server 6.3-5.02 (built Oct 12 2007; 32bit)) 8, 26 -- with ESMTPSA id {0K42001HB1SCVS00-at-zeppo.mail.uwo.pri} for 8, 26 -- microscopy-at-microscopy.com; Tue, 15 Jul 2008 12:00:12 -0400 (EDT) 8, 26 -- From: Richard Harris {rjharris-at-uwo.ca} 8, 26 -- To: Microscopy List Server {microscopy-at-microscopy.com} 8, 26 -- Subject: Job Opening for Microscopy Technologist 8, 26 -- Date: Tue, 15 Jul 2008 12:00:08 -0400 8, 26 -- Message-id: {000001c8e693$de85f0c0$9b91d240$-at-ca} 8, 26 -- X-Mailer: Microsoft Office Outlook 12.0 8, 26 -- Thread-index: Acjmk9pVRPLNRqZRQLmCWaNiHH5DQA== 8, 26 -- Content-language: en-us 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6FG0VDT018642 ==============================End of - Headers==============================
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Organization: Fred Hutchinson Cancer Research Center in Seattle, WA
Title-Subject: [Filtered] RE: Job Opening for Electron Microscopist
Question: The Fred Hutchinson Cancer Research Electron Microscopy Resource has a vacancy for an Electron Microscopist. A description of the position can be found on the MSA Placement Office /Job Openings web site. You can also go to www.fhcrc.org and the job# is KSW-21904. Any questions can be directed to Bobbie Schneider, e-mail address: bschneid-at-fhcrc.org.
Hi All- Here's one for all you immuno wizards out there..... I have a client who is studying a-Beta protein. I have done some immuno labelling of his fibrils using primary Abs he supplies and gold-tagged secondaries from Aurion (no commercial interest, etc). I adsorb the proteins onto the grids, float the grids on blocking buffer and then proceed with the primary Ab, multiple washes with the blocking buffer and then the secondary Ab (diluted in the BB) and then wash with PBS, water and then neg. stain with Uranyl acetate. I've had good results. This week I did another prep in which I labeled the proteins with 2 primaries, individually and as a double label. One primary is a mouse Mc, the other a human polyclonal IgG. Individually, each Ab labelled, but very weakly. When I did the double label, in which I pooled the primaries for the first incubation and pooled the secondaries for the second incubation, I got very strong labeling with both Abs. A control in which I used blocking buffer in place of the primaries, but used both secondaries combined, was very clean. The labeling pattern seems to agree with data from AFM in which they used the primary Abs, but no secondaries and could image the fibrils and bound primaries.
Has anyone seen a phenomenon like this before? Two Abs that seem to act synergistically? We are going to repeat the whole thing next week, with more controls, but I was really puzzled by what we saw.
All comment welcome! Lee -- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
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Question: Recently we have experienced an overload on the constant drag turbo pump on the prep chamber of our K1250 when purging the sample chamber of the transfer device. This does not happen when we PURGE the slushing chamber or the dead space. We are also unable to draw the vacuum we should *when the transfer device is attached and both valves are open on the transfer device*. It acts as if there is an internal valving or switching problem; at times I can still hear purging on the sample side when PURGE is not selected. If unnoticed, the most dramatic consequence is a dumping of the chamber vacuum on our FESEM. The vacuum line is original, and the unit is 6-7 years old.
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Question: Recently we have experienced an overload on the constant drag turbo pump on the prep chamber of our K1250 when purging the sample chamber of the transfer device. This does not happen when we PURGE the slushing chamber or the dead space. We are also unable to draw the vacuum we should *when the transfer device is attached and both valves are open on the transfer device*. It acts as if there is an internal valving or switching problem; at times I can still hear purging on the sample side when PURGE is not selected. If unnoticed, the most dramatic consequence is a dumping of the chamber vacuum on our FESEM. The vacuum line is original, and the unit is 6-7 years old.
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Question: Recently we have experienced an overload on the constant drag turbo pump on the prep chamber of our K1250 when purging the sample chamber of the transfer device. This does not happen when we PURGE the slushing chamber or the dead space. We are also unable to draw the vacuum we should *when the transfer device is attached and both valves are open on the transfer device*. It acts as if there is an internal valving or switching problem; at times I can still hear purging on the sample side when PURGE is not selected. If unnoticed, the most dramatic consequence is a dumping of the chamber vacuum on our FESEM. The vacuum line is original, and the unit is 6-7 years old.
The mail server here is under a SPAM attack, and this is adversely affecting delivery as I'm dealing with several thousand junk messages/minute. Just a heads up, that there will likely be some effects on Microscopy Listserver Email delivery until I get this under control.
The spam should not be getting through to any of you, however, the mail server is getting overloaded and as a result things are running very slowly as the MTA (mail transport agent) is choking. You may see a few duplicate mesages as I try to compensate for the bottlenecks. Hopefully I will be able to minimize this.
Sorry,
Nestor Your Very Annoyed Neighborhood SysOp
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Wed Jul 16 20:59:42 2008 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6H1xeFL023767 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jul 2008 20:59:41 -0500 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240800c4a455267588-at-[206.69.208.22]} 6, 11 -- Date: Wed, 16 Jul 2008 20:59:39 -0500 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 6, 11 -- Subject: Administrivia: Mail services under attack 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT
Title-Subject: FEG tips
Question: Hello Listers
I'm just getting a feel for "the budget" and would like to enquire about FEG tips - mainly how often you have to change yours: what instrument do you have, how many hours usage per wk or month and how often it's been changed.
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Email: w-russin-at-northwestern.edu Name: Bill Russin
Organization: Biological Imaging Facility/Northwestern University
Title-Subject: 300 kV FETEM
Question: We are going to purchase a 300 kV FETEM that will be used for cryoTEM, tomography, EDS, EELS, HAADF ñ but mainly for cryo-applications. The two scopes that we are considering are the FEI Technai F30 and the JEOL 3200 FS. To make the best choice for our facility, we'd like to hear about your experiences with either of these instruments and/or the companies that sell them. It would be helpful to know what instrument you have (or use) presently and how you would rate it as to ease-of-use, level of service, performance, stability, etc. Since the scope will be installed in a multiuser facility, any experiences you would be willing to share regarding its administration and management would be much appreciated. Thank you for your help.
FE tips typically run about $2000USD. Depending on the SEM make, they are either easy to change or difficult to change. The key suppliers are Denka and FEI.
FEI PN 17135 ca 2007- FEI PN 19030 ca 2008+ Denka TFE
The Denka starts very quickly and easily. The FEI starts slowly and sometimes, will not start. But when it does, it is very stable. The Denka is a bit less stable but 95% always run.
On Zeiss Supra 4,000 hours is not unreasonable. Vacuum needs to be in the mid E-10Torr. So cleanliness and bakeout is essential. Initially, I figured that the tip would be replaced twice a year. That is now down to about one tip over two years. But one particular tip could exhibit odd behavior and need replacing. Some start and run and then go wild. There is no tip warranty from third party suppliers. There is under a support contract.
So figure one year to be safe. SEM shots of two year old tips still show lots of ZrO2. YMMV.
gary g.
At 07:31 PM 7/16/2008, you wrote:
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Question: Hi Emer, We have two FEG instruments in the lab: HR SEM Sirion (FEI) and Tecnai F20 G2 (FEI). Both are operated from December 2002. We replace FEG tip on Sirion roughly every 1-1.5 year. But on TEM we are lucky and still !!! using the first tip. Best, Inna
On Jul 16, 2008, at 7:27 PM, emer.ryan-at-dit.ie wrote:
} I'm just getting a feel for "the budget" and would like to enquire } about FEG tips - mainly how often you have to change yours: what } instrument do you have, how many hours usage per wk or month and how } often it's been changed.
Dear Emer, Our FEG tips last about two to three years on our FEI Polara. Since we leave the HT and FEG on all the time, it doesn't matter how many hours the machine is actually collecting data--we have automated data collection software, Leginon, so data is actually being collected most of the time. We are about to change the FEG, so we are at the end of the life of our third tip, and our scope was installed in 2003. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Jul 17 11:35:36 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6HGZXfL031333 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2008 11:35:36 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id B7E692E500D5; 6, 22 -- Thu, 17 Jul 2008 09:35:31 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id BD93C2E502F0; 6, 22 -- Thu, 17 Jul 2008 09:35:30 -0700 (PDT) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 22 -- In-Reply-To: {200807170227.m6H2RmPX006834-at-ns.microscopy.com} 6, 22 -- References: {200807170227.m6H2RmPX006834-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {DEE52FB4-8892-49C5-BEF9-07DF4B807B31-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] viaWWW: FEG tips 6, 22 -- Date: Thu, 17 Jul 2008 09:35:33 -0700 6, 22 -- To: emer.ryan-at-dit.ie, microscopy-at-microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
On Jul 16, 2008, at 7:28 PM, w-russin-at-northwestern.edu wrote:
} Name: Bill Russin } } Organization: Biological Imaging Facility/Northwestern University } } Title-Subject: 300 kV FETEM } } Question: We are going to purchase a 300 kV FETEM } that will be used for cryoTEM, tomography, EDS, } EELS, HAADF ñ but mainly for cryo-applications. } The two scopes that we are considering are the } FEI Technai F30 and the JEOL 3200 FS. To make the } best choice for our facility, we'd like to hear } about your experiences with either of these } instruments and/or the companies that sell them. } It would be helpful to know what instrument you } have (or use) presently and how you would rate it } as to ease-of-use, level of service, performance, } stability, etc. Since the scope will be installed } in a multiuser facility, any experiences you } would be willing to share regarding its } administration and management would be much } appreciated. Thank you for your help.
Dear Bill, We have a FEI Tecnai TF30H Polara with an energy filter. I don't think that EDS is possible on the Polara, since there are two cryo- boxes inside the objective lens volume, which would have to be modified to allow the EDS detector to see the x-rays--someone from FEI could give a definitive answer. EDS may not be needed, however, since much the same information is available with EELS. We have been taking data for about five years, mostly tomography, but some single- particle, and the instrument has performed very well; stability is quite good also, which requires that our room meets specs on vibrations, EM fields, and especially temperature control. We have had a very good uptime record, and whenever the instrument has been down, we have had prompt and excellent service. The Tecnai software is well set up for multi-user operation, and we have found the instrument to be pretty easy to use, although some training of users is essential. We also use Leginon for automated data collection. I have no connection with FEI other than as a satisfied user. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 7, 23 -- From tivol-at-caltech.edu Thu Jul 17 12:42:00 2008 7, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6HHg0XJ014622 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2008 12:42:00 -0500 7, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 7, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id 1721966E0289 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2008 10:42:00 -0700 (PDT) 7, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 7, 23 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 7, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 25F6966E0296 7, 23 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jul 2008 10:41:59 -0700 (PDT) 7, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 7, 23 -- In-Reply-To: {200807170228.m6H2SX0o007943-at-ns.microscopy.com} 7, 23 -- References: {200807170228.m6H2SX0o007943-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 7, 23 -- Message-Id: {3AAB9FEA-CB01-430E-8E08-669FFC7BE57F-at-caltech.edu} 7, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 7, 23 -- Subject: Re: [Microscopy] viaWWW: 300 kV FETEM 7, 23 -- Date: Thu, 17 Jul 2008 10:42:01 -0700 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- X-Mailer: Apple Mail (2.753.1) 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6HHg0XJ014622 ==============================End of - Headers==============================
I just got the yearly pre-M&M flier from Leica and thought those who haven't gotten theirs (or tossed it without opening) might be interested hearing that there is an addition to Leica's catalog announced there: "Now Available! Leica HPM100: High-pressure freezing unit for EM". Sic.
I'll take it as a good news.
Vlad _____________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Many thanks to all who responded. The appraisals have been } extremely useful and much appreciated. } } As it appears that the replies have not been distributed to the } list server and following a request for info, here they are: } } Alastair } } Alastair McKinnon } IMS Histology & EM Facility Manager, R2.26 } University of Aberdeen, Institute of Medical Science } Foresterhill, Aberdeen, AB25 2ZD } tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) } fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk } www.abdn.ac.uk/ims/h-em } } ---------------------------------------------------------------------- } ----------------------- } } My experience may be a bit out of date. When I had the funds (about } } 4-5 years ago) we tested the then current Leica model against the } Bal-Tec HPM 010 extensively - and bought the Bal-Tec. We got many } more good freezes for a variety of sample types. } } More recently, I have seen superb freezes achieved by the newer } Leica model in the hands of others, so the choice may no longer be } so clear cut? } } Leica offers a lot more innovation with regard to sample holders, } and depending on the course of your work, that consideration might } be important. For using standard A + B hats, the Bal-Tec is still } outstanding. } } Good luck. } } David Hall } } ---------------------------------------------------------------------- } ------------------------- } } Subject: Re: Bal-Tec HPM100 } } Dear Alastair, } } sorry for my late reply, I was out of town. Our Bal-Tec HPM100 was } installed like four weeks ago and so far I didn't find the time to } really use it (aside from a few test runs). But I will start soon } to seriously use the machine and will in any case keep you updated } on my experience. } } About the factors that influenced my decision to go for the HPM100: } } Initially I had to make a decision between the Leica EM PACT 2 } (with RTS; rapid transfer system) and the old Bal-Tec HPM010, as at } the time when I was on "high pressure freezer shopping tour" the } Bal-Tec HPM100 was not on the market. I talked to a lot of people, } users of the Leica EM PACT and EM PACT 2 and users of the Bal-Tec } HPM010. By talking to all these people I got the impression that } when it comes to hard to freeze samples the HPM010 can give you } better results than the Leica EM PACT or EM PACT 2. So at one point } I decided to go for the HPM010. The only real disadvantage I saw } with that machine was that - compared to the Leica machines - it is } really kinda complicated to operate and needs a lot of liquid } nitrogen. But I was ready and prepared to live with that. } } Then Bal-Tec came up with the new HPM100 and I was really happy } about that new machine because I think (or maybe lets better say } hope) that it combines the pros of the old HPM010 (good freezing } results with a wide variety of biological samples) with the pros of } the Leica machine (easy to use, low consumption of liquid } nitrogen). One of the things I also really like about the HPM100 is } that the size and shape of the sample holders can be modified. So } besides the standard 2 mm sample holders there will be 5 mm sample } holders available, in my eyes a real plus. } } I am working with all kinds of samples: yeast and Chlamydomonas at } the moment beeing the most important ones, but also Drosophila, } Caenorhabditis, higher plants, mammalian tissue samples etc. Rapid } transfer for live cell co-relative study was not a factor in my } purchase decision, as with the HPM010 a rapid transfer would have } been really hard to realise. With the new HPM100 a rapid transfer } for live cell co-relative study should be absolutely doable, and as } far as I know the are developing such a system for the HPM100. } } One thing which concerns me a little is how the situation will } develop now after Bal-Tec got sold to Leica. So will Leica continue } both machines, or did they just buy Bal-Tec to get rid of a } competitor and are there plans to cease the production of the } HPM100. I am having no idea, maybe you can get some honest } informations regarding this from your Leica representative. } } I hope this helps a little. There would be way more to say but I am } in a hurry (a lot of travelling at the moment). I will be out of } town next week but back in the lab on July 21st. So if you have any } further question don't hesitate to contact me, I will be happy to } help if I can. } } As I wrote above, when I had to make the decision I talked to a lot } of people and I found it extremely helpful to get all these } comments and feedback from people actually using the machines. } } All the best, } } Stefan } } } } ---------------------------------------------------------------------- } ------------------------- } } Hi Alistair, } } We currently have two high pressure freezers at our facility; a } Wohlwend Compact 01 and the previous model Baltec HPM 010. My boss } is going to make me sell one of them. I have not decided which, } they both work very well. If you are interested purchasing used } feel free to request more information. } } Cheers, } } KD Derr } } ---------------------------------------------------------------------- } -------------------------- } } Hi Alastair, } } I have what I believe to be the oldest functioning HPM010 in the } world. [22yrs, it's used fairly infrequently] It's my experience } that the machine is built to withstand all the abuse one can } imagine. As long as the seals are regularly changed and the rest } of the maintenance is kept up, our machine works great every time. } } The design has changed very little over the years, mostly new bells } and whistles. I haven't had experience with the newest Leica } offering, but was disappointed when I initially tested the first } freezer they marketed. } } Cheers, } Jay } } } ---------------------------------------------------------------------- } --------------- } } Alastair, } } There is another and better alternative: } } http://www.technotradeinc.com/Wohlwend/hpf%20default.htm {http:// } www.technotradeinc.com/Wohlwend/hpf%20default.htm} } } This is the US distributor, but they can tell you who deals with } the UK, I'm sure. } } This is the guy who invented and developed the Baltec HPF 010. They } split, and Wohlwend set up independently. } } I used the HPF 010 at a previous position, and consider it *much* } better fhan the Leice Empact in all respects. The current version } that Wohlwend is making is an improved version of the HPF 010. } } Debby Sherman at Purdue has one of these, and as I understand likes } it very much. } } I just wish we could get one here. } } Phil } } ---------------------------------------------------------------------- } ------- } } Hello, } } I would recommend you to also consider the Wohlwend HPF Compact } 001 High pressure freezing machine. I believe that it is better } than the competing machines. I have it in use for more than 5 years } and it works well and provides great results, especially for } adherent cultivated cells. } } The suppliers email is: } martin-wohlwend-at-bluewin.ch } } With kind regards Paul Walther } } Here are some of our recent publications with the machine: } } Selection of book articles and peer reviewed papers showing results } obtained with the Wohlwend HPM Compact 01 } } Walther P. (2008) High Resolution Cryoscanning Electron Microscopy } of Biological Samples. Book article in: Schatten H, Pawley J. B. } (eds) Biological Low-Voltage Scanning Electron Microscopy. Springer } New York. ISBN 978-0-387-72970-1 (Print) 978-0-387.72972-5 } (Online). pp 245-261. Summary {http://www.springerlink.com/content/ } g53un05383l44270/} } } Schlottmann S, Buback F, Stahl B, Meierhenrich R, Walter P, } Georgieff M, Senftleben U. (2008) Prolonged Classical NF-κB } Activation Prevents Autophagy upon E. coli Stimulation In Vitro: A } Potential Resolving Mechanism of Inflammation. Mediators of } Inflamation doi:10.1155/2008/725854 (in press) } } Barbosa-Barros L, de la Maza A, Estelrich J, Linares AM, Feliz M, } Walther P, Pons R, López O. (2008) Penetration and Growth of DPPC/ } DHPC Bicelles Inside the Stratum Corneum of the Skin. Langmuir. 24, } 5700-5706 Summary {http://www.ncbi.nlm.nih.gov/pubmed/18471002? } ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pu } bmed_RVDocSum} } } Hölzle A, Fischer S, Heyer R, Schütz S, Zacharias M, Walther P, } Allers T, Marchfelder A. (2008). Maturation of the 5S rRNA 5' end } is catalyzed in vitro by the endonuclease tRNase Z in the archaeon } H. volcanii. RNA. 2008 14, 928-937 Summary {http:// } www.ncbi.nlm.nih.gov/pubmed/18369184? } ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pu } bmed_RVDocSum} } } Buser C, Walther P. (2008) Freeze-Substitution: The Addition of } Water to Polar Solvents Enhances the Retention of Structure and } Acts at Temperatures Around –60 °C. J. Microsc. 230, 268-77. } Summary {http://www.ncbi.nlm.nih.gov/pubmed/18445157? } ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pu } bmed_RVDocSum} } } Rupp B, Ruzsics Z, Buser C, Adler B, Walther P, Koszinowski UH. } (2007) A random screen for dominant-negative mutants of the } cytomegalovirus nuclear egress protein M50. J Virol. 81, 5508-5517 } Summary {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi? } db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17376929&query_hl=1 } &itool=pubmed_docsum} } } Buser C, Walther P, Mertens T, Michel D. (2007) Cytomegalovirus } primary envelopment occurs at large infoldings of the inner nuclear } membrane. } J Virol. 81, 3042-8. Summary {http://www.ncbi.nlm.nih.gov/entrez/ } query.fcgi? } db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17192309&query_hl=1 } &itool=pubmed_docsum} } } } } Buser C, Fleischer F, Mertens T, Michel D, Schmidt V, Walther P. } (2007) Quantitative investigation of murine cytomegalovirus } nucleocapsid interaction. J. Mircrosc. 228, 78-87. Summary {http:// } www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2818.2007.01825.x} } } Martin R, Hild S, Walther P, Ploss K, Boland W, Tomaschko K.-H. } (2007) Granular Chitin in the Epidermis of Nudibranch Molluscs. } Biol. Bull. 213, 307-315. Summary {http://www.ncbi.nlm.nih.gov/ } sites/entrez? } Db=pubmed&Cmd=ShowDetailView&TermToSearch=18083970&ordinalpos=1&itool= } EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum} } } Wild P, Ziegler U, Schraner EM, Senn C, Tobler K, Engels M, Loepfe } E, Ackermann M, Mueller M, Walther P. (2004) Impairment of Nuclear } Pores in Bovine Herpesvirus 1 Infected MDBK Cells. J. Virol. 79, } 1071-1083. } } Nijsse J, Walther P and Hoekstra FA. (2004) Cold-induced } imbibition damage of lettuce embryos: A study using cryo-scanning } electron microscopy. Seed Science Research, 14, 117-126. } } -------------------------------------------------------- } } you may also have a look to the Wohlwend HPF machine which to my } knowledge and experience is absolutely equivalent if not better in } all major aspects. } } If interested, please contact Paul Walther at Ulm University for } further details on this machine. } } paul.walther-at-uni-ulm.de } } best regards, } } Reinhard Rachel } } -- } } ---------------------- } } ---------------------------------------------------------------------- } ------------------------- } } Hi Alasdair, } } I just purchased an EMPACT2 from Leica and it is an amazingly } simple machine to use. It is also very reliable for reproducibility } f freezing. } } As you pointed out, sometimes it is good to have a larger specimen } planchet, but by giving this up you end up with a machine that can } produce more well-frozen specimens than the Baltec machine. I have } used both machines over the last three years and chose the EMPACT2 } for its ease of use and reproducibility. I was never allowed to use } the Baltec machine alone - only with an "expert" present (Kent } McDonald in Berkeley or Heinz Schwarz in Tuebingen). Each time it } worked well. } } However when I was introduced to the EMPACT2, it was a case of } someone showing me how to load the specimen and then how to put } this into the machine, and I was off on my own. Since then I } purchased my own machine and have had a great success with it. I } even teach high-school students how to prepare specimens with it. } } Bear in mind that historically Leica will buy up their competition, } swear that they will continue to support the machines and then } quietly phase them out (where are the LKB ultramicrotomes these } days?). } } If you really are looking for the Baltec-style machine then you } should see if the UK has a distributor for the Wohlwend HPF. } Wohlwend machines are built on the same principle as the Baltec } units and from what I hear they are very reliable. I am pretty sure } that Paul Walther in Ulm is still using one of the first ones ever } built. Maybe you should contact him for a reference. } } Good luck with your search. } } Regards, } } Paul Webster. } } } } The University of Aberdeen is a charity registered in Scotland, No } SC013683.
==============================Original Headers============================== 7, 24 -- From vladislav_speransky-at-nih.gov Thu Jul 17 15:13:19 2008 7, 24 -- Received: from nihrelayxway5.hub.nih.gov (nihrelayxway5.hub.nih.gov [128.231.90.99]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6HKDJe9001169 7, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Jul 2008 15:13:19 -0500 7, 24 -- X-IronPortListener: NIH_Relay 7, 24 -- X-SBRS: None 7, 24 -- X-IronPort-AV: E=Sophos;i="4.31,204,1215403200"; 7, 24 -- d="scan'208";a="187311275" 7, 24 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 7, 24 -- by nihrelayxway5.hub.nih.gov with ESMTP; 17 Jul 2008 16:13:19 -0400 7, 24 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 7, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6HKDH3O013586 7, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Jul 2008 16:13:17 -0400 7, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) 7, 24 -- References: {200807150807.m6F87DKA007379-at-ns.microscopy.com} 7, 24 -- Content-Type: text/plain; charset=UTF-8; delsp=yes; format=flowed 7, 24 -- Message-Id: {39399EB4-777B-423A-9145-C2B2D9C5735E-at-nih.gov} 7, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 7, 24 -- Subject: Fwd: [Microscopy] RE: Listserver HPF responses 7, 24 -- Date: Thu, 17 Jul 2008 16:12:21 -0400 7, 24 -- To: Microscopy-at-microscopy.com 7, 24 -- X-Mailer: Apple Mail (2.753.1) 7, 24 -- Content-Transfer-Encoding: 8bit 7, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6HKDJe9001169 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jacqueline.williams-at-stjude.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jacqueline.williams-at-stjude.org Name: jackie williams
Organization: St. Jude Children's Research Hospital
Title-Subject: Stereo microscope with fluorescent capabilities
Question: Would really appreciate input on a good stereo microscope with fluorescent capabilities. Need experienced input on quality for the best money and service from the company. Thanks ever so much. Jackie Williams
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbras-at-u.washington.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sbras-at-u.washington.edu Name: Scott Braswell
Organization: UW Nanotech User Facility
Title-Subject: EDAX on PDMS
Question: Greetings,
I was recently asked to perform elemental analysis (EDAX) on some Au/Pd impregnated PDMS samples. I normally prep samples by sputtering with Au/Pd metal, but in this instance I tried carbon coating them to allow quantifying the metal. Unfortunately, the carbon coat wasn't sufficient to control the charging. Short of applying 20+nm of Au/Pd, I've not found a reliable way to prep PDMS for e-beam imaging. Can anyone recommend a mounting and prep technique that works on PDMS?
I tried running EDAX on the charging samples and found no Au/Pd peaks present, but I've been assured that there is infact metal present
We are seeking for applications for the position of an electron microscopy facility leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany. The Max Planck Society is an independent non-profit research organization. The institute hosts 25 research groups mainly focussing on cell biology and tissue formation, supported by a variety of well-equipped facilities. To view the job description and details for an application, please go to http://www.mpi-cbg.de/research/jobs/em.html.
Best regards, Michaela
Dr. Michaela Wilsch-Braeuninger Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauer Str. 108 01307 Dresden Germany Phone: +49 (351) 210 2629 (1759, 1488) Fax: +49 (351) 210 2000 email: wilsch-at-mpi-cbg.de
==============================Original Headers============================== 4, 16 -- From wilsch-at-mpi-cbg.de Fri Jul 18 03:08:48 2008 4, 16 -- Received: from mail-srv3.mpi-cbg.de (mail-srv3.mpi-cbg.de [141.5.10.13]) 4, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6I88mhR028639 4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jul 2008 03:08:48 -0500 4, 16 -- Received: from [10.1.130.17] (ppc-em-4-u39.mpi-cbg.de [10.1.130.17]) 4, 16 -- by mail-srv3.mpi-cbg.de (Postfix) with ESMTPSA id B71B92AFC121 4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jul 2008 10:08:47 +0200 (CEST) 4, 16 -- Mime-Version: 1.0 (Apple Message framework v753.1) 4, 16 -- Content-Transfer-Encoding: 7bit 4, 16 -- Message-Id: {EE5A2C8A-478F-4FCF-950C-BE8CBB5976B7-at-mpi-cbg.de} 4, 16 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- From: Michaela Wilsch-Braeuninger {wilsch-at-mpi-cbg.de} 4, 16 -- Subject: job opening: TEM facility leader, Dresden, Germany 4, 16 -- Date: Fri, 18 Jul 2008 10:10:12 +0200 4, 16 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Hi Jackie Here at the Imaging Unit of UWO's Biotron we chose a Zeiss Lumar V12 stereo microscope with 3 channel fluorescence after much research and evaluation. I love the image quality, magnification and resolution (up to 150X with sub-micrometer resolution!). Our version is fully digital controlled though it is available with standard manual controls as well. We chose the Fluor-Arc HBO 100 self centering lamphouse and control for fluorescence illumination.
Rick,
Richard Harris, Manager - Imaging and Data Systems The Biotron - Experimental Climate Change Research University of Western Ontario, London Ontario, CANADA. N6A 5B7 Ph. 519-661-2111 ext. 86780 Fax 519-661-3935 e-mail rjharris-at-uwo.ca web: www.biotron.uwo.ca
==============================Original Headers============================== 7, 27 -- From rjharris-at-uwo.ca Fri Jul 18 07:48:05 2008 7, 27 -- Received: from uwo.ca (v320-146-lb.its.uwo.ca [129.100.74.146]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6ICm2UQ018110 7, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 18 Jul 2008 07:48:03 -0500 7, 27 -- MIME-version: 1.0 7, 27 -- Content-type: text/plain; charset=iso-8859-1 7, 27 -- Received: from zeppo.mail.uwo.pri (salk.mail.uwo.pri [172.29.32.41]) 7, 27 -- by zeppo.mail.uwo.pri 7, 27 -- (Sun Java(tm) System Messaging Server 6.3-5.02 (built Oct 12 2007; 32bit)) 7, 27 -- with ESMTP id {0K470095LCW1HD70-at-zeppo.mail.uwo.pri} for 7, 27 -- Microscopy-at-MSA.Microscopy.Com; Fri, 18 Jul 2008 08:48:01 -0400 (EDT) 7, 27 -- Received: from rjbook (rjbook.biotron.uwo.ca [129.100.52.17]) 7, 27 -- by zeppo.mail.uwo.pri 7, 27 -- (Sun Java(tm) System Messaging Server 6.3-5.02 (built Oct 12 2007; 32bit)) 7, 27 -- with ESMTPSA id {0K4700JHYCW09S20-at-zeppo.mail.uwo.pri} for 7, 27 -- Microscopy-at-MSA.Microscopy.Com; Fri, 18 Jul 2008 08:48:01 -0400 (EDT) 7, 27 -- From: Richard Harris {rjharris-at-uwo.ca} 7, 27 -- To: jacqueline.williams-at-stjude.org 7, 27 -- Cc: MSA Listserver {Microscopy-at-MSA.Microscopy.Com} 7, 27 -- Subject: Fluourescence Stereo'scope 7, 27 -- Date: Fri, 18 Jul 2008 08:48:00 -0400 7, 27 -- Message-id: {002a01c8e8d4$8317e070$8947a150$-at-ca} 7, 27 -- X-Mailer: Microsoft Office Outlook 12.0 7, 27 -- Content-language: en-us 7, 27 -- Thread-index: Acjo1IKqcMIknpSGSXmLzjXCZRT2Mw== 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6ICm2UQ018110 ==============================End of - Headers==============================
Dear David, Yes, it works just fine. The only difference is that the polymerized resin will easily peel away from both layers of ACLAR, so you can simply cut the resin. Make a thicker drop of resin between the layers of ACLAR so it will be easier to work with. Good luck, Jo Dee
~~Jo Dee Fish~~ Research Technologist III The J. David Gladstone Institutes Co-manager Histology and Microscopy Core
Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158
-----Original Message----- X-from: David Lowry [mailto:dlowry-at-asu.edu] Sent: Friday, July 18, 2008 6:39 AM To: jfish-at-gladstone.ucsf.edu
Here's what I do. I embed the Thermanox coverslip inverted (cell side down) on a square of ACLAR (bought from Ted Pella or Electron Microscopy Sciences) over a drop of Eponate 12. Then I polymerize this as usual. After polymerization I remove the ACLAR, it peels away with no effort. Then I use a razor blade and remove small rectangles, about 2mm X 3mm, from the coverslip, about 3 or 4 pieces. Don't cut completely through the coverslip or it is nearly impossible to remove from the resin. I then use the corner of the razor blade to peel the resin from the coverslip. Pour Eponate 12 into the regular silicone mold and then individually place the cut rectangles in the end of the mold creating a stack. Polymerize the blocks. When you section them you will get a nice cross section of several cell layers and they will not split apart. Good luck, Jo Dee
~~Jo Dee Fish~~ Research Technologist III The J. David Gladstone Institutes Co-manager Histology and Microscopy Core
Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158
-----Original Message----- X-from: mgengle-at-email.uky.edu [mailto:mgengle-at-email.uky.edu] Sent: Friday, July 11, 2008 9:41 AM To: jfish-at-gladstone.ucsf.edu
Dear Listers,
We have a customer who has grown cells on Thermonox and needs to see them on edge, so I embedded the thermonox in flat molds in Eponate 12. However when sectioning, the thermonox pulled away from the resin. The cells are then just sort of hanging out in space or the edge of the section folds over on itself so the cells are inside the fold. Can anyone recommend a resin or another way to embed the cells so they'll stay embedded and not separate when sectioning? I tried picking them up on formvar but would like to avoid that if possible.
Thanks in advance, Mary Gail Engle
Mary Gail Engle Sr Research Facility Manager Electron Microscopy & Imaging Facility HSRB rm 001 ph (859) 323-6108 FAX (859) 323-8089 BBSRB rm o74 Ph (859)323-2701 University of KY
==============================Original Headers============================== 7, 26 -- From mgengle-at-email.uky.edu Fri Jul 11 11:37:38 2008 7, 26 -- Received: from uksmtp3.uky.edu (uksmtp3.uky.edu [128.163.16.36]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BGbc5S014283 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:37:38 -0500 7, 26 -- Received: from EX7HB03.ad.uky.edu (Ex7hb03.ad.uky.edu [128.163.187.55]) 7, 26 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) 7, 26 -- (No client certificate requested) 7, 26 -- by uksmtp3.uky.edu (Postfix) with ESMTP id 1580B10609E9 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:28:54 -0400 (EDT) 7, 26 -- Received: from EX7FM03.ad.uky.edu ([128.163.187.12]) by EX7HB03.ad.uky.edu 7, 26 -- ([128.163.187.55]) with mapi; Fri, 11 Jul 2008 12:37:37 -0400 7, 26 -- From: "Engle, Mary" {mgengle-at-email.uky.edu} 7, 26 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 7, 26 -- Date: Fri, 11 Jul 2008 12:37:36 -0400 7, 26 -- Subject: 7, 26 -- Thread-Index: AcjjdG0pU9/j4iVoTVCcxHyLxcdjTQ== 7, 26 -- Message-ID: {DADA8E000C493F4BB3357F27DBF0A8570906119D71-at-EX7FM03.ad.uky.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en-US 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="us-ascii" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6BGbc5S014283 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 28 -- From jfish-at-gladstone.ucsf.edu Fri Jul 11 11:56:03 2008 16, 28 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6BGu2eb007875 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jul 2008 11:56:02 -0500 16, 28 -- X-IronPort-AV: E=Sophos;i="4.30,346,1212390000"; 16, 28 -- d="scan'208";a="919822" 16, 28 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25]) 16, 28 -- by gmail2.ucsf.edu with ESMTP; 11 Jul 2008 09:56:02 -0700 16, 28 -- Received: from [169.230.76.4] (HELO JFISH) 16, 28 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 4.2.10) 16, 28 -- with ESMTP id 565536600; Fri, 11 Jul 2008 09:56:01 -0700 16, 28 -- Reply-To: {jfish-at-gladstone.ucsf.edu} 16, 28 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu} 16, 28 -- To: {mgengle-at-email.uky.edu} 16, 28 -- Cc: {microscopy-at-microscopy.com} 16, 28 -- References: {200807111641.m6BGfIKU023483-at-ns.microscopy.com} 16, 28 -- Subject: RE: [Microscopy] 16, 28 -- Date: Fri, 11 Jul 2008 09:55:58 -0700 16, 28 -- Organization: J. David Gladstone Institutes 16, 28 -- Message-ID: {000001c8e376$fe225c90$2e0d010a-at-JFISH} 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Type: text/plain; 16, 28 -- charset="us-ascii" 16, 28 -- Content-Transfer-Encoding: 7bit 16, 28 -- X-Mailer: Microsoft Office Outlook 11 16, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 28 -- Thread-Index: AcjjdQqvNnxmC/JuQNa7AjMLUQnDkQAAEwgg 16, 28 -- In-Reply-To: {200807111641.m6BGfIKU023483-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 35, 28 -- From jfish-at-gladstone.ucsf.edu Fri Jul 18 10:37:37 2008 35, 28 -- Received: from gmail2.ucsf.edu (gmail2.ucsf.edu [169.230.76.31]) 35, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6IFbb9Q005335 35, 28 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jul 2008 10:37:37 -0500 35, 28 -- X-IronPort-AV: E=Sophos;i="4.31,210,1215414000"; 35, 28 -- d="scan'208";a="945308" 35, 28 -- Received: from unknown (HELO gladstone.ucsf.edu) ([172.17.1.25]) 35, 28 -- by gmail2.ucsf.edu with ESMTP; 18 Jul 2008 08:37:28 -0700 35, 28 -- Received: from [169.230.76.4] (HELO JFISH) 35, 28 -- by gladstone.ucsf.edu (CommuniGate Pro SMTP 4.2.10) 35, 28 -- with ESMTP id 565570182; Fri, 18 Jul 2008 08:37:28 -0700 35, 28 -- Reply-To: {jfish-at-gladstone.ucsf.edu} 35, 28 -- From: "Jo Dee Fish" {jfish-at-gladstone.ucsf.edu} 35, 28 -- To: "'David Lowry'" {dlowry-at-asu.edu} 35, 28 -- Cc: {microscopy-at-microscopy.com} 35, 28 -- References: {200807111658.m6BGwU4C014232-at-ns.microscopy.com} {B9366E69DACF09458B402E4C4385012604B2ABA5-at-EX03.asurite.ad.asu.edu} 35, 28 -- Subject: RE: [Microscopy] RE: embedding monolayer cells 35, 28 -- Date: Fri, 18 Jul 2008 08:37:25 -0700 35, 28 -- Organization: J. David Gladstone Institutes 35, 28 -- Message-ID: {002c01c8e8ec$2dae9080$2e0d010a-at-JFISH} 35, 28 -- MIME-Version: 1.0 35, 28 -- Content-Type: text/plain; 35, 28 -- charset="us-ascii" 35, 28 -- Content-Transfer-Encoding: 7bit 35, 28 -- X-Mailer: Microsoft Office Outlook 11 35, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 35, 28 -- thread-index: Acjjdyslz0R9/2kkSUOgHC3h2MgUewFYtWfSAASCJdA= 35, 28 -- In-Reply-To: {B9366E69DACF09458B402E4C4385012604B2ABA5-at-EX03.asurite.ad.asu.edu} ==============================End of - Headers==============================
You did not specify a few things which are probably important. Let me pose some questions back to you.
You did not specify the conditions used for the analysis. I also assume you are using an SEM instead of a TEM, but maybe you should confirm that.
How much Au and Pd is supposedly impregnated in these samples. I would think a couple of tenths of a percent would be easily detectable. I presume you are looking at the M lines around 2 keV in an SEM and the L lines if you are using a TEM.
I presume you can readily see peaks for Au and Pd when you coat with those elements. 20 nm is a fairly thick coat. I would think you could get by with half of that, but you should still see the peaks.
Carbon coating should prevent charging. Personally, I prefer the high-vacuum carbon evaporators as opposed to the flash units. PDMS might also be more challenging than other materials.
Charging may inhibit decent imaging, but it should not prevent EDS unless it is VERY severe. What is your effective accelerating voltage? Where does the spectral background run out on the high end? What kind of count rate were you able to attain? Optimum conditions for EDS and imaging are seldom the same.
How is the Au-Pd distributed? I presume it would be present as small islands or globules. Are those visible in the sample? Have you tried both SE and BSE imaging?
I hope this helps and gets you closer to solving your problem.
Warren S. Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: sbras-at-u.washington.edu [mailto:sbras-at-u.washington.edu] Sent: Thursday, July 17, 2008 5:46 PM To: wesaia-at-iastate.edu
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Email: sbras-at-u.washington.edu Name: Scott Braswell
Organization: UW Nanotech User Facility
Title-Subject: EDAX on PDMS
Question: Greetings,
I was recently asked to perform elemental analysis (EDAX) on some Au/Pd impregnated PDMS samples. I normally prep samples by sputtering with Au/Pd metal, but in this instance I tried carbon coating them to allow quantifying the metal. Unfortunately, the carbon coat wasn't sufficient to control the charging. Short of applying 20+nm of Au/Pd, I've not found a reliable way to prep PDMS for e-beam imaging. Can anyone recommend a mounting and prep technique that works on PDMS?
I tried running EDAX on the charging samples and found no Au/Pd peaks present, but I've been assured that there is infact metal present
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Email: jacqueline.williams-at-stjude.org Name: jackie williams
Organization: St. Jude Children's Research Hospital
Title-Subject: [Filtered] EM - immunogold help
Question: I was really grateful for all the help with the stereo microscope. It is good to be able to draw upon other people's knowledge. I am a beginner in the electron microscopy immunogold labeling. I am trying the pre and post embedding using epon. What I would love to have (if possible) is a relatively consistent antibody to kinda- sorta use as a control so I can be relatively sure I am doing this right. Is there any such antibody or antibodies? Once again, thanks for any knowledge you are willing to share. Jackie Williams
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Email: tsitrin-at-sbcglobal.net Name: Sam Tsitrin
Organization: Oakland Charter High
Title-Subject: [Filtered] Donation for High School Microscopes
Question: Dear MSA community- I am a beginning Biology teacher in one of the worst performing districts in the country: Oakland , CA. (one of the worst drop-out rates in the nation!). I teach in a small public charter school and my students actually do care about science. But, alas we don't have much funds or equipment yet. I am trying to equpii our classroom before the coming year and am working with a school donation site Donorschoose where anyone charitable can donate to help us get microscopes. Please consider helping a new generation of students learn about microscopy and visit http://www.donorschoose.org/donors/proposal.html?id=190292 for more information.
so far $100 has been donated, but we need $1000 to fund the microscopes. ANy amount would be appreciated!
Paula Sicurello VA San Diego Healthcare System Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
--- On Sat, 7/19/08, jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} wrote:
} From: jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} } Subject: [Microscopy] viaWWW: EM - immunogold help } To: vapatpxs-at-yahoo.com } Date: Saturday, July 19, 2008, 2:38 PM } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy } Listserver } using the WWW based Form at } http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both jacqueline.williams-at-stjude.org as well } as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: jacqueline.williams-at-stjude.org } Name: jackie williams } } Organization: St. Jude Children's Research Hospital } } Title-Subject: [Filtered] EM - immunogold help } } Question: I was really grateful for all the help with the } stereo } microscope. It is good to be able to draw upon other } people's } knowledge. I am a beginner in the electron microscopy } immunogold } labeling. I am trying the pre and post embedding using } epon. What I } would love to have (if possible) is a relatively consistent } antibody } to kinda- sorta use as a control so I can be relatively } sure I am } doing this right. Is there any such antibody or antibodies? } Once again, thanks for any knowledge you are willing to } share. Jackie Williams } } Login Host: 192.55.208.10 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Sat Jul 19 09:24:21 } 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id m6JEOJH8003974 } 6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 19 } Jul 2008 09:24:20 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: } {p06240802c4a7a90a22c6-at-[206.69.208.22]} } 6, 11 -- Date: Sat, 19 Jul 2008 09:24:18 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: EM - immunogold help } 6, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 26 -- From vapatpxs-at-yahoo.com Sat Jul 19 10:32:28 2008 10, 26 -- Received: from n28.bullet.mail.mud.yahoo.com (n28.bullet.mail.mud.yahoo.com [68.142.206.223]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6JFWSxF031481 10, 26 -- for {microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 10:32:28 -0500 10, 26 -- Received: from [68.142.194.243] by n28.bullet.mail.mud.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [216.252.122.218] by t1.bullet.mud.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [69.147.65.163] by t3.bullet.sp1.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [127.0.0.1] by omp408.mail.sp1.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- X-Yahoo-Newman-Property: ymail-3 10, 26 -- X-Yahoo-Newman-Id: 720313.67637.bm-at-omp408.mail.sp1.yahoo.com 10, 26 -- Received: (qmail 25917 invoked by uid 60001); 19 Jul 2008 15:32:27 -0000 10, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 26 -- s=s1024; d=yahoo.com; 10, 26 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Message-ID; 10, 26 -- b=nX+X1UddNayunUrMTHzrbktjTv0Br4YNwDtWkDWjzwXwTV3/0cMxwuGIXOVLXBzNq9dTHzy/I3BqYwITMLtaed7ctPHELEa5LhH2cGZUxpz7jQXjyaMWhMdQodbPMzpik2jJELju8nBl9qsQh0HSF62GnOB5dmFS5cJAMyCrN/w=; 10, 26 -- Received: from [24.30.157.32] by web46112.mail.sp1.yahoo.com via HTTP; Sat, 19 Jul 2008 08:32:27 PDT 10, 26 -- X-Mailer: YahooMailWebService/0.7.218 10, 26 -- Date: Sat, 19 Jul 2008 08:32:27 -0700 (PDT) 10, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 26 -- Reply-To: vapatpxs-at-yahoo.com 10, 26 -- Subject: Re: [Microscopy] viaWWW: EM - immunogold help 10, 26 -- To: jacqueline.williams-at-stjude.org, MSA BB {Microscopy-at-microscopy.com} 10, 26 -- In-Reply-To: {200807191438.m6JEcPLp028956-at-ns.microscopy.com} 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; charset=us-ascii 10, 26 -- Message-ID: {622075.25766.qm-at-web46112.mail.sp1.yahoo.com} ==============================End of - Headers==============================
We have a Philips EM410LS for sale. 100kV acceleration voltage, Tungsten filament, 1k Gatan CCD camera. The instrument is complete, including water chiller, sample holder, negative holder box and PC for the Gatan CCD camera. The microscope is from 1986, and was under regular Philips/FEI maintenance contract until 2005. Currently, the vacuum system doesn't start completely, which might be an electronic problem, but we did not investigate the reason for this. Otherwise, the instrument is fully operational, as far as we know. The Gatan CCD camera dates from 1996. The instrument is for sale for $5000, transport is at buyers' cost.
The instrument can be seen here: http://stahlberglab.ucdavis.edu/EM410LS-UCDavis.jpg/view
If you are interested, please contact me for further information.
Henning Stahlberg, Molecular & Cellular Biology, Briggs Hall 5, University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab), Fax: +1-530-752 3085 mailto:HStahlberg-at-ucdavis.edu http://stahlberglab.ucdavis.edu http://2dx.org
==============================Original Headers============================== 9, 21 -- From HStahlberg-at-ucdavis.edu Sat Jul 19 14:30:44 2008 9, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6JJUh1Y021324 9, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 14:30:43 -0500 9, 21 -- Received: from [192.168.1.125] (adsl-75-30-69-9.dsl.scrm01.sbcglobal.net [75.30.69.9]) 9, 21 -- (authenticated bits=0) 9, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id m6JJUd9B029526 9, 21 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 9, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 12:30:42 -0700 (PDT) 9, 21 -- Message-Id: {F6A1BA1F-9364-4F5F-AEBF-44A9261D4E5F-at-ucdavis.edu} 9, 21 -- From: Henning Stahlberg {HStahlberg-at-ucdavis.edu} 9, 21 -- To: Microscopy-at-microscopy.com 9, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 21 -- Content-Transfer-Encoding: 7bit 9, 21 -- Mime-Version: 1.0 (Apple Message framework v928.1) 9, 21 -- Subject: Philips EM410LS with Gatan 1k CCD for sale, Northern California 9, 21 -- Date: Sat, 19 Jul 2008 12:30:42 -0700 9, 21 -- X-Mailer: Apple Mail (2.928.1) 9, 21 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av8 9, 21 -- X-Virus-Status: Clean 9, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 ==============================End of - Headers==============================
The problem with using anti-actin is that actin is everywhere so it is hard to tell if you have a lot of cytoplasmic staining (though there shouldn't be any gold on top of the organelles. I would recommend a membrane localized stain. A gold-conjugated lectin (e.g., wheat germ agglutinin) is an easy thing to use and it should label lots of plasma membranes and mucin in goblet cells of most species. If you want a true antibody to work out the technique with, it would depend on your tissue and the species.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] Sent: Saturday, July 19, 2008 10:33 AM To: Phillips, Thomas E.
Hi Jackie,
I used to use an anti-actin antibody.
Paula :-)
Paula Sicurello VA San Diego Healthcare System Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
--- On Sat, 7/19/08, jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} wrote:
} From: jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} } Subject: [Microscopy] viaWWW: EM - immunogold help } To: vapatpxs-at-yahoo.com } Date: Saturday, July 19, 2008, 2:38 PM } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } This Question/Comment was submitted to the Microscopy } Listserver } using the WWW based Form at } http://microscopy.com/MLFormMail.html } ------------------------------------------------------------------------ --- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both jacqueline.williams-at-stjude.org as well } as the } MIcroscopy Listserver } ------------------------------------------------------------------------ --- } } Email: jacqueline.williams-at-stjude.org } Name: jackie williams } } Organization: St. Jude Children's Research Hospital } } Title-Subject: [Filtered] EM - immunogold help } } Question: I was really grateful for all the help with the } stereo } microscope. It is good to be able to draw upon other } people's } knowledge. I am a beginner in the electron microscopy } immunogold } labeling. I am trying the pre and post embedding using } epon. What I } would love to have (if possible) is a relatively consistent } antibody } to kinda- sorta use as a control so I can be relatively } sure I am } doing this right. Is there any such antibody or antibodies? } Once again, thanks for any knowledge you are willing to } share. Jackie Williams } } Login Host: 192.55.208.10 } ------------------------------------------------------------------------ --- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Sat Jul 19 09:24:21 } 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id m6JEOJH8003974 } 6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 19 } Jul 2008 09:24:20 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: } {p06240802c4a7a90a22c6-at-[206.69.208.22]} } 6, 11 -- Date: Sat, 19 Jul 2008 09:24:18 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: EM - immunogold help } 6, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 26 -- From vapatpxs-at-yahoo.com Sat Jul 19 10:32:28 2008 10, 26 -- Received: from n28.bullet.mail.mud.yahoo.com (n28.bullet.mail.mud.yahoo.com [68.142.206.223]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6JFWSxF031481 10, 26 -- for {microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 10:32:28 -0500 10, 26 -- Received: from [68.142.194.243] by n28.bullet.mail.mud.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [216.252.122.218] by t1.bullet.mud.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [69.147.65.163] by t3.bullet.sp1.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [127.0.0.1] by omp408.mail.sp1.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- X-Yahoo-Newman-Property: ymail-3 10, 26 -- X-Yahoo-Newman-Id: 720313.67637.bm-at-omp408.mail.sp1.yahoo.com 10, 26 -- Received: (qmail 25917 invoked by uid 60001); 19 Jul 2008 15:32:27 -0000 10, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 26 -- s=s1024; d=yahoo.com; 10, 26 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Versi on:Content-Type:Message-ID; 10, 26 -- b=nX+X1UddNayunUrMTHzrbktjTv0Br4YNwDtWkDWjzwXwTV3/0cMxwuGIXOVLXBzNq9dTHz y/I3BqYwITMLtaed7ctPHELEa5LhH2cGZUxpz7jQXjyaMWhMdQodbPMzpik2jJELju8nBl9q sQh0HSF62GnOB5dmFS5cJAMyCrN/w=; 10, 26 -- Received: from [24.30.157.32] by web46112.mail.sp1.yahoo.com via HTTP; Sat, 19 Jul 2008 08:32:27 PDT 10, 26 -- X-Mailer: YahooMailWebService/0.7.218 10, 26 -- Date: Sat, 19 Jul 2008 08:32:27 -0700 (PDT) 10, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 26 -- Reply-To: vapatpxs-at-yahoo.com 10, 26 -- Subject: Re: [Microscopy] viaWWW: EM - immunogold help 10, 26 -- To: jacqueline.williams-at-stjude.org, MSA BB {Microscopy-at-microscopy.com} 10, 26 -- In-Reply-To: {200807191438.m6JEcPLp028956-at-ns.microscopy.com} 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; charset=us-ascii 10, 26 -- Message-ID: {622075.25766.qm-at-web46112.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From PhillipsT-at-missouri.edu Sat Jul 19 16:32:44 2008 20, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6JLWipe004847 20, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 16:32:44 -0500 20, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 20, 26 -- Sat, 19 Jul 2008 16:32:44 -0500 20, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 26 -- Content-class: urn:content-classes:message 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- Subject: RE: [Microscopy] Re: viaWWW: EM - immunogold help 20, 26 -- Date: Sat, 19 Jul 2008 16:32:29 -0500 20, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD048B6506-at-UM-XMAIL06.um.umsystem.edu} 20, 26 -- In-Reply-To: {200807191533.m6JFXCGq032635-at-ns.microscopy.com} 20, 26 -- X-MS-Has-Attach: 20, 26 -- X-MS-TNEF-Correlator: 20, 26 -- Thread-Topic: [Microscopy] Re: viaWWW: EM - immunogold help 20, 26 -- Thread-Index: AcjptMIa/W7JANzdT8689gHw194XxwAMbjOw 20, 26 -- References: {200807191533.m6JFXCGq032635-at-ns.microscopy.com} 20, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 20, 26 -- To: {vapatpxs-at-yahoo.com} , {Microscopy-at-microscopy.com} , 20, 26 -- {jacqueline.williams-at-stjude.org} 20, 26 -- X-OriginalArrivalTime: 19 Jul 2008 21:32:44.0198 (UTC) FILETIME=[FAF21C60:01C8E9E6] 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6JLWipe004847 ==============================End of - Headers==============================
Has anybody any experience with Deben AMT16000 camera at 35 mm side port? We are considering to install a camera at 35 mm port on our FEI Tecnai 12 Biotwin and want to have sincere opinions. Our other option would be Orius SC1000.
Regards, -- Aleksandr Mironov Experimental Officer D.1527, M.Smith Building EM Core Facility, Faculty of Life Sciences University of Manchester Oxford Road Manchester M13 9PT UK
Personally, I think you'd be far better off asking for equipment donations and using the cash to help pay for shipping. Money is hard to come by - used, but still working and usable equipment is available in many storage closets, and would be a lot easier for most of us (well..., me, anyway) to get our hands on and get permission to donate. While I think it would be great for your students to have brand-new equipment, wouldn't it be better to have more, older equipment than a few pieces that are brand-new? You might try contacting local industries, also - if they're anything like the places I've worked in, they have a stash of equipment stored as back-ups that they could be talked into donating. In the process, you'd make contacts that could be useful for guest speakers, project sponsors or even internships - it's been my experience that most industrial labs would love to help out the local schools, but don't know how, or even who to contact.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company 3 Sharpie Way Shelbyville, TN 37160 Direct: +1 (931) 685-6635 Fax: +1 (931) 685-6613 robert.zonis-at-sanford.com www.papermate.com www.sharpie.com
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Email: U.J.Potter-at-bath.ac.uk Name: Ursula Potter
Organization: University of Bath Uk
Title-Subject: [Filtered] Black precipitate
Question: Dear All,
Many thanks to those of you who gave advice regarding black precipitate in sperm preparations for TEM. I am now fairly certain that the problem occured because of inadequate washes between pre & post fixation. The sperm pellet was very tiny and easily disturbed - washing such a sample in an eppendorf is not easy! Some other tissue I prepared a few days following the sperm prep is fine with no trace of precipitate at all. We are going to try high pressure freezing and freze substitution with the sperm.
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Email: grb-at-ufl.edu Name: Gerald Bourne
Organization: University of Florida, Major Analytical Instrumentation Center
Title-Subject: [Filtered] User log software
Question: Dear all,
I am interested in obtaining a user login software for Microsoft operating systems that will lock all functions of the computer yet keep all programs running. I would like this software to keep a log of user's time. This would have to run on multiple Windows platforms as we have microscopes running everything from Win 3.1 to Win Xp. If there is not something that will cover such a broad range of operating systems, I am especially interested in Win NT.
Any input is appreciated,
Gerald Bourne, Ph.D. Major Analytical Instrumentation Center Department of Materials Science and Engineering University of Florida 109 MAE P.O. Box 116400 Gainesville, FL 32611 (352) 392-3077 (352) 392-0390 fax
Most commercial polyclonal Abs to tubulin will bind to microtubules (to keep the microtubules from depolymerization, do not fix in cold aldehyde). A second choice, anti-cytochrome oxidase will label mitochondria - sorry don't remember the brand I used.
Vlad _______________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Question: I was really grateful for all the help with the stereo } microscope. It is good to be able to draw upon other people's } knowledge. I am a beginner in the electron microscopy immunogold } labeling. I am trying the pre and post embedding using epon. What I } would love to have (if possible) is a relatively consistent antibody } to kinda- sorta use as a control so I can be relatively sure I am } doing this right. Is there any such antibody or antibodies? } Once again, thanks for any knowledge you are willing to share. } Jackie Williams } } Login Host: 192.55.208.10 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Sat Jul 19 09:24:21 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m6JEOJH8003974 } 6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 } 09:24:20 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240802c4a7a90a22c6-at-[206.69.208.22]} } 6, 11 -- Date: Sat, 19 Jul 2008 09:24:18 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: EM - immunogold help } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 23 -- From vladislav_speransky-at-nih.gov Mon Jul 21 13:57:41 2008 5, 23 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6LIvcxl013492 5, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jul 2008 13:57:40 -0500 5, 23 -- X-IronPortListener: NIH_Relay 5, 23 -- X-SBRS: None 5, 23 -- X-IronPort-AV: E=Sophos;i="4.31,225,1215403200"; 5, 23 -- d="scan'208";a="22176976" 5, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 23 -- by nihrelayxway3.hub.nih.gov with ESMTP; 21 Jul 2008 14:57:35 -0400 5, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6LIvDFJ028255 5, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jul 2008 14:57:15 -0400 5, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 5, 23 -- References: {200807191426.m6JEQZht006773-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 23 -- Message-Id: {54D4FB82-778E-401C-9B59-5B2D7707F154-at-nih.gov} 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 23 -- Subject: Fwd: [Microscopy] viaWWW: EM - immunogold help 5, 23 -- Date: Mon, 21 Jul 2008 14:56:14 -0400 5, 23 -- To: Microscopy-at-microscopy.com 5, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
Dear colleagues it is available for free: Mazza D, Braeckmans K, Cella F, Testa I, Vercauteren D, Demeester J, De Smedt SS, Diaspro A. A New FRAP/FRAPa Method For 3-D Diffusion Measurements Based On Multi-photon Excitation Microscopy. Biophys J. 2008.
http://www.biophysj.org All the best Alby
---------------------------------------------------- "Water slowly flowed under the sky" (Cesare Pavese) ----------------------------------------------------- Alberto Diaspro, EBSA President-Elect, MicroScoBIO LAMBS-IFOM, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy - fax +39-010314218 - tel +39 0103536426/309; URLs: www.lambs.it ; EBSA is Biophysics in Europe - European Biophysical Societies' Association www.ebsa.org ----------------------------------------------
==============================Original Headers============================== 8, 22 -- From sekkio-at-mac.com Mon Jul 21 15:50:38 2008 8, 22 -- Received: from asmtpout019.mac.com (asmtpout019.mac.com [17.148.16.94]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6LKocRq029963 8, 22 -- for {Microscopy-at-Microscopy.Com} ; Mon, 21 Jul 2008 15:50:38 -0500 8, 22 -- MIME-version: 1.0 8, 22 -- Content-transfer-encoding: 7BIT 8, 22 -- Content-type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 22 -- Received: from [10.0.1.4] ([87.15.122.195]) 8, 22 -- by asmtp019.mac.com (Sun Java(tm) System Messaging Server 6.3-6.03 (built Mar 8, 22 -- 14 2008; 32bit)) with ESMTPSA id {0K4D00LX1J7OBN42-at-asmtp019.mac.com} for 8, 22 -- Microscopy-at-Microscopy.Com; Mon, 21 Jul 2008 13:50:15 -0700 (PDT) 8, 22 -- Sender: sekkio-at-mac.com 8, 22 -- Message-id: {D7C39225-A1BD-4F29-A936-9F642AA79067-at-mac.com} 8, 22 -- From: Diaspro {sekkio-at-mac.com} 8, 22 -- To: Confocal Microscopy List {CONFOCAL-at-LISTSERV.BUFFALO.EDU} , 8, 22 -- mplsm-users-at-yahoogroups.com, Microscopy-at-Microscopy.Com 8, 22 -- In-reply-to: {273DB49A-7056-4640-A959-E35FF5AB7B3C-at-fhcrc.org} 8, 22 -- Subject: FRAP 8, 22 -- Date: Mon, 21 Jul 2008 22:50:11 +0200 8, 22 -- References: {4884756D.4070901-at-ucl.ac.uk} 8, 22 -- {273DB49A-7056-4640-A959-E35FF5AB7B3C-at-fhcrc.org} 8, 22 -- X-Mailer: Apple Mail (2.928.1) ==============================End of - Headers==============================
Our lab is looking to replace an old video camera on our petrographic microscope (a Zeiss AxioPlan) with a new digital camera.
The majority of work done on this microscope is transmitted- and polarized-light petrography. We've found that this can be challenging for digital cameras because, for example, thin sections can have very bright quartz grains next to nearly black garnets. We don't, on the other hand, do any fluorescence work. Superior performance for petrography is, accordingly, a requirement.
Other than that, we don't need anything too fancy. Because this is a multi-user microscope, ease of use and general versatility is more important than special features. All we really need is a good live feed to view, a good still-image capture, and a scale bar on both of those. As for cost, we are looking for low to moderately priced models (unless the arguments for higher-end models are so persuasive that the department head and other research groups can be convinced to chip in).
Suggestions based on users' experiences (either positive or negative) are welcome -- feel free to respond to me off-list.
Dealers are welcome to submit product literature and a cost estimate, but a demo or loaned unit will eventually be required for consideration.
Thanks, Ellery
----------------- Ellery Frahm Manager & Principal Analyst, Electron Microprobe Lab Research Fellow, Department of Geology & Geophysics University of Minnesota - Twin Cities http://probelab.geo.umn.edu
Please note: Sometimes the University's spam filters are over- protective and reject wanted messages, especially from free or overseas accounts. If your message is rejected or you are concerned that you did not receive a reply from me, please feel free to try my alternate email account: elleryfrahm-at-mac.com
==============================Original Headers============================== 10, 17 -- From frah0010-at-umn.edu Tue Jul 22 14:45:49 2008 10, 17 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6MJjl7M013162 10, 17 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jul 2008 14:45:48 -0500 10, 17 -- Received: from 212-swing.geo.umn.edu (212-swing.geo.umn.edu [160.94.146.133]) 10, 17 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 10, 17 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jul 2008 14:45:41 -0500 (CDT) 10, 17 -- X-Umn-Remote-Mta: [N] 212-swing.geo.umn.edu [160.94.146.133] #+LO+TS+AU 10, 17 -- Message-Id: {7C2749C3-6EF7-48FB-BCE5-8560F2673F6B-at-umn.edu} 10, 17 -- From: Ellery Frahm {frah0010-at-umn.edu} 10, 17 -- To: microscopy-at-microscopy.com 10, 17 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 10, 17 -- Content-Transfer-Encoding: 7bit 10, 17 -- Mime-Version: 1.0 (Apple Message framework v928.1) 10, 17 -- Subject: Digital camera for petrographic scope 10, 17 -- Date: Tue, 22 Jul 2008 14:45:40 -0500 10, 17 -- X-Mailer: Apple Mail (2.928.1) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jacqueline.williams-at-stjude.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jacqueline.williams-at-stjude.org Name: jackie williams
Organization: St. Jude Children's Research Hospital
Title-Subject: [Filtered] EM - printer which prints good EM photos on paper
Question: Looking for suggestions on good printer to print EM images on plain print paper for survey. Any suggestions? Thanks, Jackie
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Phil.Ahrenkiel-at-sdsmt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Phil.Ahrenkiel-at-sdsmt.edu Name: Phil Ahrenkiel
Organization: South Dakota School of Mines & Technology
Title-Subject: [Filtered] RT embedding
Question: We are looking for a media to embed polymeric (PVP) nanofibers in at room temperature. We have tried Araldite and Spurrís resin, but the metallic (titanium) precursor in the nanofibers begins to nucleate grains during curing (around 60 degC), whereas we want to section the pristine material. So we would like to try an embedding media that cures without heating, maybe using UV. Could anyone suggest something for this? It might spare us some trial-and-error. Thanks.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ech-at-interchange.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] M & M 2008 The Family Affair
Question: The Family Affair
In accordance with the enthusiastic consensus of the Family Affair from last year, this year more hands-on for the participants has been arranged. The two sessions are open to anyone but are designed for lay experience of families (especially children) and friends of the delegates and teachers.
The sessions are 1:30 - 3:30 pm. Registration is not required, go directly to the Santa Ana room.
On Tuesday, August 5, the MAS IMS group (leaders Jaret Frafjord and Frauke Hogue) will provide a mostly materials session with some exciting experiments
* Examine metallographic sections and fracture surfaces with the microscopes * Observe changes in materials that are frozen in liquid nitrogen * Guess unknown materials through various testing * Roll pennies very flat * Create brass pennies * Demonstrate super hydro-phobic (afraid of water) materials
On Wednesday, August 6, with leader Elaine Humphrey, we will have "CSI Albuquerque."
CSI Albuquerque The story so far:
The Governor is coming to Albuquerque to evaluate whether to award $7 million to the university as one of two candidates for the prize. The University wants to upgrade its aging microscopy facility. With this money they will be able to build a world class microscopy facility open to anyone in the state. The Governor is also a huge fan of the Albuquerque Isotope Baseball team and rarely misses a game.
Disaster has struck the day before he is due to arrive. Someone has stolen the Albuquerque Isotope's baseball trophy. The Governor is due to meet the team and is scheduled to have his photograph taken with the cup and the team tomorrow.
Fortunately, the Microscopy Society of America is having their conference in Albuquerque this week. That means that the vendors have brought the most sophisticated and latest equipment to the State, and they are making it available to Dr. Elaine Humphrey and her team (The Family Affair).
By the time we come into the story the police have five suspects in view. We need your help to aid Dr. Humphrey to find out who did it, so the cup can be recovered in time for the Governors meeting tomorrow. You are being asked to collect the clues, prepare slides and stubs and take photographs of the specimens and piece together what happened.
Our testing still shows the Epson C88plus is the best plain paper printer. Current cost is $85. Nothing beats it on plain paper However, if you want work prints the *NEW *HP laserjet with Colorsphere technology produces B/W prints at resolutions never before seen in laserjet. The model of choice is the HP 3600n ($400) or the 3600dn ($600) (double sided printing) We have network version and print 1 meg images in 10 sec start to finish 4 Meg images take approx 40 sec. The prints have a blue tint but the resolution,density, and color gamut are amazing. What is different is that they are using wax in the toner. This eliminates the banding in the output entirely. Do not second guess this model choice. The cheaper models don't work. period Any older model that does not have colorsphere toner don't come close to this new technology. Feel free to contact me off-list if you have questions
John
-- John M. Mackenzie, Jr., PhD Professor of Microbiology Coordinator, Center for Electron Microscopy North Carolina State University Phone (919) 515-2664 Fax (919) 515-8293
==============================Original Headers============================== 6, 17 -- From john_mackenzie-at-ncsu.edu Tue Jul 22 22:57:54 2008 6, 17 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6N3vsnp014279 6, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Jul 2008 22:57:54 -0500 6, 17 -- Received: from [127.0.0.1] (really [66.57.53.232]) 6, 17 -- by cdptpa-omta02.mail.rr.com with ESMTP 6, 17 -- id {20080723035753.QNCQ15817.cdptpa-omta02.mail.rr.com-at-[127.0.0.1]} 6, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 03:57:53 +0000 6, 17 -- Message-ID: {4886ACBC.8080008-at-ncsu.edu} 6, 17 -- Date: Tue, 22 Jul 2008 23:59:56 -0400 6, 17 -- From: John Mackenzie {john_mackenzie-at-ncsu.edu} 6, 17 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 6, 17 -- MIME-Version: 1.0 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- Subject: Re: Best printer for B/W on plain pare. 6, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi! An elegant and efficient immunolabel involves the use of BrU or BrdU incorporation and subsequent detection on sections with a highly specific and efficient anti-BrdU antibody. BrdU labels newly-synthesized DNA strands, BrU labels nascent RNAs (even in 3D!). It may sound complicated but it is quite straigthforward and efficient, the antibody (I think Boehringer, now Roche developped it) being really good. These labelings on sections give nice pictures, with contrasted chromatin constellated with gold particles. If you want to see a nice job with BrU: http://www.jcb.org/cgi/content/full/157/5/743 For BrdU: http://www.ingentaconnect.com/content/ap/ex/2000/00000260/00000002/art04999;jsessionid=ffnapd2p4t3jh.alice?format=print No personal interest in JCB or Exp. Cell Res. :-)
Best regards, Stephane
----- Original Message ---- X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} To: nizets2-at-yahoo.com Sent: Saturday, July 19, 2008 11:37:06 PM
The problem with using anti-actin is that actin is everywhere so it is hard to tell if you have a lot of cytoplasmic staining (though there shouldn't be any gold on top of the organelles. I would recommend a membrane localized stain. A gold-conjugated lectin (e.g., wheat germ agglutinin) is an easy thing to use and it should label lots of plasma membranes and mucin in goblet cells of most species. If you want a true antibody to work out the technique with, it would depend on your tissue and the species.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] Sent: Saturday, July 19, 2008 10:33 AM To: Phillips, Thomas E.
Hi Jackie,
I used to use an anti-actin antibody.
Paula :-)
Paula Sicurello VA San Diego Healthcare System Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
--- On Sat, 7/19/08, jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} wrote:
} From: jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} } Subject: [Microscopy] viaWWW: EM - immunogold help } To: vapatpxs-at-yahoo.com } Date: Saturday, July 19, 2008, 2:38 PM } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } This Question/Comment was submitted to the Microscopy } Listserver } using the WWW based Form at } http://microscopy.com/MLFormMail.html } ------------------------------------------------------------------------ --- } Remember this posting is most likely not from a Subscriber, } so when replying } please copy both jacqueline.williams-at-stjude.org as well } as the } MIcroscopy Listserver } ------------------------------------------------------------------------ --- } } Email: jacqueline.williams-at-stjude.org } Name: jackie williams } } Organization: St. Jude Children's Research Hospital } } Title-Subject: [Filtered] EM - immunogold help } } Question: I was really grateful for all the help with the } stereo } microscope. It is good to be able to draw upon other } people's } knowledge. I am a beginner in the electron microscopy } immunogold } labeling. I am trying the pre and post embedding using } epon. What I } would love to have (if possible) is a relatively consistent } antibody } to kinda- sorta use as a control so I can be relatively } sure I am } doing this right. Is there any such antibody or antibodies? } Once again, thanks for any knowledge you are willing to } share. Jackie Williams } } Login Host: 192.55.208.10 } ------------------------------------------------------------------------ --- } } ==============================Original } Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Sat Jul 19 09:24:21 } 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id m6JEOJH8003974 } 6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 19 } Jul 2008 09:24:20 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: } {p06240802c4a7a90a22c6-at-[206.69.208.22]} } 6, 11 -- Date: Sat, 19 Jul 2008 09:24:18 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of } MicroscopyListserver) } 6, 11 -- Subject: viaWWW: EM - immunogold help } 6, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 26 -- From vapatpxs-at-yahoo.com Sat Jul 19 10:32:28 2008 10, 26 -- Received: from n28.bullet.mail.mud.yahoo.com (n28.bullet.mail.mud.yahoo.com [68.142.206.223]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6JFWSxF031481 10, 26 -- for {microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 10:32:28 -0500 10, 26 -- Received: from [68.142.194.243] by n28.bullet.mail.mud.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [216.252.122.218] by t1.bullet.mud.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [69.147.65.163] by t3.bullet.sp1.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- Received: from [127.0.0.1] by omp408.mail.sp1.yahoo.com with NNFMP; 19 Jul 2008 15:32:27 -0000 10, 26 -- X-Yahoo-Newman-Property: ymail-3 10, 26 -- X-Yahoo-Newman-Id: 720313.67637.bm-at-omp408.mail.sp1.yahoo.com 10, 26 -- Received: (qmail 25917 invoked by uid 60001); 19 Jul 2008 15:32:27 -0000 10, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 26 -- s=s1024; d=yahoo.com; 10, 26 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Versi on:Content-Type:Message-ID; 10, 26 -- b=nX+X1UddNayunUrMTHzrbktjTv0Br4YNwDtWkDWjzwXwTV3/0cMxwuGIXOVLXBzNq9dTHz y/I3BqYwITMLtaed7ctPHELEa5LhH2cGZUxpz7jQXjyaMWhMdQodbPMzpik2jJELju8nBl9q sQh0HSF62GnOB5dmFS5cJAMyCrN/w=; 10, 26 -- Received: from [24.30.157.32] by web46112.mail.sp1.yahoo.com via HTTP; Sat, 19 Jul 2008 08:32:27 PDT 10, 26 -- X-Mailer: YahooMailWebService/0.7.218 10, 26 -- Date: Sat, 19 Jul 2008 08:32:27 -0700 (PDT) 10, 26 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 10, 26 -- Reply-To: vapatpxs-at-yahoo.com 10, 26 -- Subject: Re: [Microscopy] viaWWW: EM - immunogold help 10, 26 -- To: jacqueline.williams-at-stjude.org, MSA BB {Microscopy-at-microscopy.com} 10, 26 -- In-Reply-To: {200807191438.m6JEcPLp028956-at-ns.microscopy.com} 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; charset=us-ascii 10, 26 -- Message-ID: {622075.25766.qm-at-web46112.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From PhillipsT-at-missouri.edu Sat Jul 19 16:32:44 2008 20, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6JLWipe004847 20, 26 -- for {Microscopy-at-microscopy.com} ; Sat, 19 Jul 2008 16:32:44 -0500 20, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 20, 26 -- Sat, 19 Jul 2008 16:32:44 -0500 20, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 26 -- Content-class: urn:content-classes:message 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- Subject: RE: [Microscopy] Re: viaWWW: EM - immunogold help 20, 26 -- Date: Sat, 19 Jul 2008 16:32:29 -0500 20, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD048B6506-at-UM-XMAIL06.um.umsystem.edu} 20, 26 -- In-Reply-To: {200807191533.m6JFXCGq032635-at-ns.microscopy.com} 20, 26 -- X-MS-Has-Attach: 20, 26 -- X-MS-TNEF-Correlator: 20, 26 -- Thread-Topic: [Microscopy] Re: viaWWW: EM - immunogold help 20, 26 -- Thread-Index: AcjptMIa/W7JANzdT8689gHw194XxwAMbjOw 20, 26 -- References: {200807191533.m6JFXCGq032635-at-ns.microscopy.com} 20, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 20, 26 -- To: {vapatpxs-at-yahoo.com} , {Microscopy-at-microscopy.com} , 20, 26 -- {jacqueline.williams-at-stjude.org} 20, 26 -- X-OriginalArrivalTime: 19 Jul 2008 21:32:44.0198 (UTC) FILETIME=[FAF21C60:01C8E9E6] 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6JLWipe004847 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 20 -- From nizets2-at-yahoo.com Wed Jul 23 04:42:44 2008 34, 20 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 34, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6N9giTq005566 34, 20 -- for {microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 04:42:44 -0500 34, 20 -- Received: (qmail 82513 invoked by uid 60001); 23 Jul 2008 09:42:44 -0000 34, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 34, 20 -- s=s1024; d=yahoo.com; 34, 20 -- h=Received:X-Mailer:Date:From:Subject:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 34, 20 -- b=lgzVJawJCbL0xaek/9KBZ43vFw7z3iMTJByDN1GV3/V8h+nAm/8LS2Nmjg3FvK7tEIHcyV6phwXzIQNMT74lOT/+NWqPPcyL30v099ZK1aoiMx+QD35fvZG3sQgqpHq6jQwKh7hAGQT4jKwQ+/Z2uYs5tBZU+PrZlFT+PENm1B4=; 34, 20 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Wed, 23 Jul 2008 02:42:43 PDT 34, 20 -- X-Mailer: YahooMailRC/1042.40 YahooMailWebService/0.7.218 34, 20 -- Date: Wed, 23 Jul 2008 02:42:43 -0700 (PDT) 34, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 34, 20 -- Subject: Re: [Microscopy] viaWWW: EM - immunogold help 34, 20 -- Cc: microscopy-at-microscopy.com 34, 20 -- MIME-Version: 1.0 34, 20 -- Content-Type: text/plain; charset=iso-8859-1 34, 20 -- Message-ID: {999709.81881.qm-at-web37408.mail.mud.yahoo.com} 34, 20 -- Content-Transfer-Encoding: 8bit 34, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6N9giTq005566 ==============================End of - Headers==============================
I was afraid of this: I will be covering many new advances in Sunday Short Course on Digital Imaging:
The HP is close to the Epson C88plus on plain paper in color The B/W is good but the Epson plus has better resolution and the B/W prints do not have strong tint. I use Hammermill Laser Print 24 lb 96 brightness about $8 per ream (500) For high end publication quality and all photography in B/W and Color it is the Epson R2400 It cost about $800 Our testing .. You can't beat it on special paper. However... Epson just introduced the Epson R2880 to replace the R2400. Epson has never updated their flagship printer so quickly before. The reason is yet another critical patent. They have a special coating on the print head that eliminates clogging problems. They have a way to check the nozzles before it prints and fix it. If that wasn't enough then they also seriously improved the color gamut of the printer in the reds (magenta) It can also print thick stock and CD's. This printer is so new that it is not even installed yet. It's in my lab next door and I'll be testing it today. I should have results by Meeting but since it is replacing the best of the best, it's from the Epson team, and it solves three out of the two most important problems with my R2400, I have 99.99999% confidence it will be outstanding. ( Three out of two was not a mistype. They added automatic noozle check to an $800 printer) Our photographic prints are now usually on 13" x 19". You can examine at a distance of 5 cm and it will look as good as color print (the Kodak photographic kind) B/W uses three blacks and achieves about 600 Pixel per inch resolution.without metamerism (color shift in different light) For those who don't know, this printer has no equal. The 3880, 4880,7880,9880 all use exactly the same technology. For the photographers in the bunch we are using premium luster paper.The prints are 100 year archival for color and 200 year archival for B/W (Wilhelm Research Institute) The cost of ink is about 15 cents on full coverage color, about 20 for the 13 x 19. Paper runs about 50-60 cents for luster. For those who don't know my lab, we have actual measured all that I state.
I am probably opening a can of worms here .
Oh yes critically important
1. Never use refilled ink NEVER 2. Never use refilled cartridges 3. Always use Epson papers in Epson printers. 4. Never set printers to economy mode.
.
John
-- John Mackenzie, Jr. Coordinator for the Center for Electron Microscopy Professor of Microbiology North Carolina State University Phone (919) 515-2664 Fax (919) 515-8293
==============================Original Headers============================== 10, 19 -- From john_mackenzie-at-ncsu.edu Wed Jul 23 08:55:37 2008 10, 19 -- Received: from uni11mr.unity.ncsu.edu (uni11mr.unity.ncsu.edu [152.1.224.170]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6NDtbHH028513 10, 19 -- for {microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 08:55:37 -0500 10, 19 -- Received: from [127.0.0.1] (cord1.mbio.ncsu.edu [152.1.178.41]) 10, 19 -- by uni11mr.unity.ncsu.edu (8.13.7/8.13.8/Nv5.2008.0610.1) with ESMTP id m6NDtYEX009079 10, 19 -- for {microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 09:55:35 -0400 (EDT) 10, 19 -- Message-ID: {48873858.4020501-at-ncsu.edu} 10, 19 -- Date: Wed, 23 Jul 2008 09:55:36 -0400 10, 19 -- From: John Mackenzie {john_mackenzie-at-ncsu.edu} 10, 19 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 10, 19 -- MIME-Version: 1.0 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- Subject: Re: Printers part 2 10, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit 10, 19 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: 2.5.2.313940, Antispam-Data: 2008.7.23.133610 10, 19 -- X-Spam-Status: No, Hits=7% 10, 19 -- X-Spam-Level: IIIIIII ==============================End of - Headers==============================
Dear John, Very interesting, your posts. Thanks a lot. A few more questions:
What happens if water (e.g. coffee) drops on the ink-jet prints? Will all of them dissolve? Laser printer pages don't. How long does a Epson high-quality ink-jet page on Epson high-quality paper have to dry, before I can put it into a plastic pocket without having it stick to it a few days later?
Dear Group: do you have any recommendations for a scanning all sorts of different size negatives - 35 mm film strip negatives, slides and EM negatives? Thanks in advance for any suggestions. Barb
==============================Original Headers============================== 1, 19 -- From maloneyb-at-fiu.edu Wed Jul 23 11:40:13 2008 1, 19 -- Received: from fmailhost05.isp.att.net (fmailhost05.isp.att.net [207.115.11.55]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6NGeDdt028363 1, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 11:40:13 -0500 1, 19 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 19 -- by isp.att.net (frfwmhc05) with ESMTP 1, 19 -- id {20080723164012H0500ebrl7e} ; Wed, 23 Jul 2008 16:40:12 +0000 1, 19 -- X-Originating-IP: [74.166.189.137] 1, 19 -- Message-ID: {48875EC9.8030300-at-fiu.edu} 1, 19 -- Disposition-Notification-To: barbara maloney {maloneyb-at-fiu.edu} 1, 19 -- Date: Wed, 23 Jul 2008 12:39:37 -0400 1, 19 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 19 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 1, 19 -- MIME-Version: 1.0 1, 19 -- To: Microscopy-at-microscopy.com 1, 19 -- Subject: scanner for EM negatives 1, 19 -- X-Priority: 2 (High) 1, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have been very happy with my Epson Perfection V700. It has holders for all kinds of negatives. I do extensive scanning of 35mm slides and TEM negatives.
David
On Jul 23, 2008, at 9:43 AM, maloneyb-at-fiu.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear Group: do you have any recommendations for a scanning all } sorts of } different size negatives - 35 mm film strip negatives, slides and EM } negatives? } Thanks in advance for any suggestions. } Barb } } ==============================Original } Headers============================== } 1, 19 -- From maloneyb-at-fiu.edu Wed Jul 23 11:40:13 2008 } 1, 19 -- Received: from fmailhost05.isp.att.net } (fmailhost05.isp.att.net [207.115.11.55]) } 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m6NGeDdt028363 } 1, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 } 11:40:13 -0500 } 1, 19 -- Received: from [192.168.1.2] } (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) } 1, 19 -- by isp.att.net (frfwmhc05) with ESMTP } 1, 19 -- id {20080723164012H0500ebrl7e} ; Wed, 23 Jul 2008 } 16:40:12 +0000 } 1, 19 -- X-Originating-IP: [74.166.189.137] } 1, 19 -- Message-ID: {48875EC9.8030300-at-fiu.edu} } 1, 19 -- Disposition-Notification-To: barbara maloney } {maloneyb-at-fiu.edu} } 1, 19 -- Date: Wed, 23 Jul 2008 12:39:37 -0400 } 1, 19 -- From: barbara maloney {maloneyb-at-fiu.edu} } 1, 19 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) } 1, 19 -- MIME-Version: 1.0 } 1, 19 -- To: Microscopy-at-microscopy.com } 1, 19 -- Subject: scanner for EM negatives } 1, 19 -- X-Priority: 2 (High) } 1, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 1, 19 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 22 -- From Elliott-at-arizona.edu Wed Jul 23 12:28:51 2008 6, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6NHSoH7010703 6, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Wed, 23 Jul 2008 12:28:51 -0500 6, 22 -- Received: from gandalfs_amavis (amavis5.email.arizona.edu [10.0.0.208]) 6, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 767BA65C2B6 6, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Wed, 23 Jul 2008 10:28:50 -0700 (MST) 6, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 6, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 13A7065C049 6, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Wed, 23 Jul 2008 10:28:49 -0700 (MST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 22 -- In-Reply-To: {200807231643.m6NGhM7i000397-at-ns.microscopy.com} 6, 22 -- References: {200807231643.m6NGhM7i000397-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {00782ADB-D38F-4188-8A8E-CB288A85DD88-at-arizona.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: David Elliott {Elliott-at-arizona.edu} 6, 22 -- Subject: Re: [Microscopy] scanner for EM negatives 6, 22 -- Date: Wed, 23 Jul 2008 10:28:48 -0700 6, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.753.1) 6, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I asked the same question over 4 years ago, the majority suggested the Microteck ArtixScan 1800f. I scan all types of neg and haven't had any problem. Good luck.
--
Sue Tyler Biologist JHT Inc. Contracting Cooperative Oxford Laboratory Center for Coastal Environmental Health & Biomolecular Research at Charleston (CCHEBR) USDOC/NOAA/NOS/NCCOS 904 South Morris St. Oxford, Maryland 21654-9724 410-226-5193 FAX: 410-226-5925 Email: Sue.Tyler-at-noaa.gov
==============================Original Headers============================== 5, 17 -- From Sue.Tyler-at-noaa.gov Wed Jul 23 12:47:13 2008 5, 17 -- Received: from mmp2.nems.noaa.gov (mmp2.nems.noaa.gov [140.90.121.157]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6NHlCel024285 5, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 12:47:13 -0500 5, 17 -- Received: from [10.60.12.198] by mmp2.nems.noaa.gov 5, 17 -- (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 2006)) 5, 17 -- with ESMTPSA id {0K4H000KH02NM160-at-mmp2.nems.noaa.gov} for 5, 17 -- Microscopy-at-microscopy.com; Wed, 23 Jul 2008 13:47:12 -0400 (EDT) 5, 17 -- Date: Wed, 23 Jul 2008 13:47:10 -0400 5, 17 -- From: Sue Tyler {Sue.Tyler-at-noaa.gov} 5, 17 -- Subject: em scanner 5, 17 -- To: Microscopy-at-microscopy.com 5, 17 -- Message-id: {48876E9E.40604-at-noaa.gov} 5, 17 -- MIME-version: 1.0 5, 17 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 5, 17 -- Content-transfer-encoding: 7BIT 5, 17 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) ==============================End of - Headers==============================
I have mineralized cell culture in scaffolds, embedded in OCT (and previously frozen). Researcher is interested in mineral and collagen, so ice damage to cells is not important. I need to process these specimens for TEM. I am not sure how embed them, whether I should try to dissolve OCT with water and for how long.
Thank you,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
X-from: Nestor J. Zaluzec - Chair MSA Standards Subcommittee
Re: MSA Standards Subcommittee Meeting during MM2008 Topic: Open Meeting on Spectral Imaging File Formats
Epon and Araldite, or their mixtures, do polymerize at temperatures lower than 60C, and as far as I know, that polymerization reaction is not exothermic (am I wrong?). Needless to say, such polymerization is usually avoided, because blocks tend to come out soft. Many refer 65C. For your situation, however, it seems reasonable to try, say 45C, having mixed the resin according to the "hard" recipe. I would still stay away from Spurr.
Acrylics, on the other hand, do generate a lot of heat during polymerization. Some are formulated, though, to polymerize at low temperatures - as low as minus 60C. The most established, "respected" one is Lowicryl HM-20. It is relatively easy to make a DIY setup, with a box of dry ice and some kind of ethanol-filled heat sink to put your capsules in. It is important to get the UV light of right wavelength and intensity. EM suppliers offer ready setups for ~$900, I think.
Another potential choice I just remembered - LR Gold, if they still sell it (not LR White, you don't want that one!). LR Gold was developed for LM but has proven useful for immunoEM as well. It cuts very nicely.
Both Lowicryl HM20 and LR Gold are well covered in literature, but please don't hesitate to ask more questions if you get confused.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Email: Phil.Ahrenkiel-at-sdsmt.edu } Name: Phil Ahrenkiel } } Organization: South Dakota School of Mines & Technology } } Title-Subject: [Filtered] RT embedding } } Question: We are looking for a media to embed } polymeric (PVP) nanofibers in at room } temperature. We have tried Araldite and Spurrís } resin, but the metallic (titanium) precursor in } the nanofibers begins to nucleate grains during } curing (around 60 degC), whereas we want to } section the pristine material. So we would like } to try an embedding media that cures without } heating, maybe using UV. Could anyone suggest } something for this? It might spare us some } trial-and-error. Thanks. } } Login Host: 12.163.29.67 } ---------------------------------------------------------------------- } ----- } } } ==============================Original } Headers============================== } 7, 13 -- From zaluzec-at-microscopy.com Tue Jul 22 21:47:13 2008 } 7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m6N2lANp007593 } 7, 13 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jul 2008 } 21:47:11 -0500 } 7, 13 -- Mime-Version: 1.0 } 7, 13 -- Message-Id: {p06240803c4ac4c0e038f-at-[206.69.208.22]} } 7, 13 -- Date: Tue, 22 Jul 2008 21:47:10 -0500 } 7, 13 -- To: microscopy-at-microscopy.com } 7, 13 -- From: Phil.Ahrenkiel-at-sdsmt.edu (by way of } MicroscopyListserver) } 7, 13 -- Subject: viaWWW: RT embedding } 7, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; } format="flowed" } 7, 13 -- Content-Transfer-Encoding: 8bit } 7, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m6N2lANp007593 } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 24 -- From vladislav_speransky-at-nih.gov Wed Jul 23 19:23:49 2008 9, 24 -- Received: from nihrelayxway5.hub.nih.gov (nihrelayxway5.hub.nih.gov [128.231.90.99]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6O0Nnua011271 9, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 19:23:49 -0500 9, 24 -- X-IronPortListener: NIH_Relay 9, 24 -- X-SBRS: None 9, 24 -- X-IronPort-AV: E=Sophos;i="4.31,241,1215403200"; 9, 24 -- d="scan'208";a="188203917" 9, 24 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 9, 24 -- by nihrelayxway5.hub.nih.gov with ESMTP; 23 Jul 2008 20:23:49 -0400 9, 24 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 9, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6O0Nmva023897 9, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 20:23:49 -0400 9, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) 9, 24 -- References: {200807230247.m6N2lv8v009735-at-ns.microscopy.com} 9, 24 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 9, 24 -- Message-Id: {5111C766-0280-4026-BFD8-3222D86F12F7-at-nih.gov} 9, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 9, 24 -- Subject: Fwd: [Microscopy] viaWWW: RT embedding 9, 24 -- Date: Wed, 23 Jul 2008 20:22:48 -0400 9, 24 -- To: Microscopy-at-microscopy.com 9, 24 -- X-Mailer: Apple Mail (2.753.1) 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6O0Nnua011271 ==============================End of - Headers==============================
Hi Stephane, thanks for a nice example. I do have interest in work coming out of Ivan Raska's lab. He's been one of the pioneers of cryo immunogold EM labeling and visited the labs where I worked a few times... I would be hesitant, though, to recommend this procedure as an easy positive control.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Hi! } An elegant and efficient immunolabel involves the use of BrU or } BrdU incorporation and subsequent detection on sections with a } highly specific and efficient anti-BrdU antibody. BrdU labels newly- } synthesized DNA strands, BrU labels nascent RNAs (even in 3D!). } It may sound complicated but it is quite straigthforward and } efficient, the antibody (I think Boehringer, now Roche developped } it) being really good. } These labelings on sections give nice pictures, with contrasted } chromatin constellated with gold particles. } If you want to see a nice job with BrU: } http://www.jcb.org/cgi/content/full/157/5/743 } For BrdU: } http://www.ingentaconnect.com/content/ap/ex/2000/00000260/00000002/ } art04999;jsessionid=ffnapd2p4t3jh.alice?format=print } No personal interest in JCB or Exp. Cell Res. :-) } } Best regards, } Stephane } } } } ----- Original Message ---- } X-from: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} } To: nizets2-at-yahoo.com } Sent: Saturday, July 19, 2008 11:37:06 PM } Subject: [Microscopy] viaWWW: EM - immunogold help } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } The problem with using anti-actin is that actin is everywhere so it is } hard to tell if you have a lot of cytoplasmic staining (though there } shouldn't be any gold on top of the organelles. I would recommend a } membrane localized stain. A gold-conjugated lectin (e.g., wheat germ } agglutinin) is an easy thing to use and it should label lots of plasma } membranes and mucin in goblet cells of most species. If you want a } true } antibody to work out the technique with, it would depend on your } tissue } and the species. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } -----Original Message----- } X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] } Sent: Saturday, July 19, 2008 10:33 AM } To: Phillips, Thomas E. } Subject: [Microscopy] Re: viaWWW: EM - immunogold help } } } } } ---------------------------------------------------------------------- } -- } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } -- } ---- } } Hi Jackie, } } I used to use an anti-actin antibody. } } Paula :-) } } Paula Sicurello } VA San Diego Healthcare System } Microscope Facility, room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } --- On Sat, 7/19/08, jacqueline.williams-at-stjude.org } {jacqueline.williams-at-stjude.org} wrote: } } } From: jacqueline.williams-at-stjude.org {jacqueline.williams-at-stjude.org} } } Subject: [Microscopy] viaWWW: EM - immunogold help } } To: vapatpxs-at-yahoo.com } } Date: Saturday, July 19, 2008, 2:38 PM } } } ---------------------------------------------------------------------- } -- } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------- } -- } ---- } } } } This Question/Comment was submitted to the Microscopy } } Listserver } } using the WWW based Form at } } http://microscopy.com/MLFormMail.html } } } ---------------------------------------------------------------------- } -- } --- } } Remember this posting is most likely not from a Subscriber, } } so when replying } } please copy both jacqueline.williams-at-stjude.org as well } } as the } } MIcroscopy Listserver } } } ---------------------------------------------------------------------- } -- } --- } } } } Email: jacqueline.williams-at-stjude.org } } Name: jackie williams } } } } Organization: St. Jude Children's Research Hospital } } } } Title-Subject: [Filtered] EM - immunogold help } } } } Question: I was really grateful for all the help with the } } stereo } } microscope. It is good to be able to draw upon other } } people's } } knowledge. I am a beginner in the electron microscopy } } immunogold } } labeling. I am trying the pre and post embedding using } } epon. What I } } would love to have (if possible) is a relatively consistent } } antibody } } to kinda- sorta use as a control so I can be relatively } } sure I am } } doing this right. Is there any such antibody or antibodies? } } Once again, thanks for any knowledge you are willing to } } share. Jackie Williams } } } } Login Host: 192.55.208.10 } } } ---------------------------------------------------------------------- } -- } --- } } } } ==============================Original } } Headers============================== } } 6, 11 -- From zaluzec-at-microscopy.com Sat Jul 19 09:24:21 } } 2008 } } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } with ESMTP id m6JEOJH8003974 } } 6, 11 -- for {microscopy-at-microscopy.com} ; Sat, 19 } } Jul 2008 09:24:20 -0500 } } 6, 11 -- Mime-Version: 1.0 } } 6, 11 -- Message-Id: } } {p06240802c4a7a90a22c6-at-[206.69.208.22]} } } 6, 11 -- Date: Sat, 19 Jul 2008 09:24:18 -0500 } } 6, 11 -- To: microscopy-at-microscopy.com } } 6, 11 -- From: jacqueline.williams-at-stjude.org (by way of } } MicroscopyListserver) } } 6, 11 -- Subject: viaWWW: EM - immunogold help } } 6, 11 -- Content-Type: text/plain; } } charset="us-ascii" ; format="flowed" } } ==============================End of - } } Headers============================== } } } } } } ==============================Original } Headers============================== } 10, 26 -- From vapatpxs-at-yahoo.com Sat Jul 19 10:32:28 2008 } 10, 26 -- Received: from n28.bullet.mail.mud.yahoo.com } (n28.bullet.mail.mud.yahoo.com [68.142.206.223]) } 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m6JFWSxF031481 } 10, 26 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 5, 23 -- From vladislav_speransky-at-nih.gov Wed Jul 23 19:32:35 2008 5, 23 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6O0WZgs023929 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 19:32:35 -0500 5, 23 -- X-IronPortListener: NIH_Relay 5, 23 -- X-SBRS: None 5, 23 -- X-IronPort-AV: E=Sophos;i="4.31,241,1215403200"; 5, 23 -- d="scan'208";a="29441865" 5, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 23 -- by nihrelayxway2.hub.nih.gov with ESMTP; 23 Jul 2008 20:32:32 -0400 5, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6O0WVbC024386 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 20:32:31 -0400 5, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 5, 23 -- References: {200807230945.m6N9jCoj006994-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 23 -- Message-Id: {F43909C0-ECE2-4C6F-90AA-95FF2EC67081-at-nih.gov} 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 23 -- Subject: Fwd: [Microscopy] Re: viaWWW: EM - immunogold help 5, 23 -- Date: Wed, 23 Jul 2008 20:31:30 -0400 5, 23 -- To: Microscopy-at-microscopy.com 5, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dlowry-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State Universty
Title-Subject: [Filtered] GFP antibody for immuno-TEM
Question: can anyone recommend a good source for an anti-GFP antibody for on-section labeling? Thank you,
Only morphology of cells is not important. Morphology of collagen and mineral is important. And I have only one specimen, so I should be careful not to let it to fall apart.
Valdimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: Stephane Nizet [mailto:nizets2-at-yahoo.com] } Sent: Thursday, July 24, 2008 3:19 AM } To: Dusevich, Vladimir } Subject: Re: [Microscopy] } } Hi Vladimir! } I am really curious to know why your researcher is interested } in EM analysis if the morphology is not important?! } Analytical chemistry is probably much more efficient in this case. } To answer your question, I wonder why it should be a problem } to dissolve OCT in water. You may consider starting from } scratch, I mean fixing in glut. } } Stephane } } } } ----- Original Message ---- } From: "DusevichV-at-umkc.edu" {DusevichV-at-umkc.edu} } To: nizets2-at-yahoo.com } Sent: Wednesday, July 23, 2008 8:34:56 PM } Subject: [Microscopy] } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Dear Listers, } } I have mineralized cell culture in scaffolds, embedded in OCT } (and previously frozen). Researcher is interested in mineral } and collagen, so ice damage to cells is not important. I need } to process these specimens for TEM. I am not sure how embed } them, whether I should try to dissolve OCT with water and for } how long. } } Thank you, } } Vladimir } } Vladimir M. Dusevich, Ph.D. } Electron Microscope Lab Manager } 371 School of Dentistry } 650 E. 25th Street } Kansas City, MO 64108-2784 } } Phone: (816) 235-2072 } Fax: (816) 235-5524 } Web: http://www.umkc.edu/dentistry/microscopy } } } } ==============================Original } Headers============================== } 8, 26 -- From DusevichV-at-umkc.edu Wed Jul 23 13:27:59 2008 8, } 26 -- Received: from kc-msxproto3.kc.umkc.edu } (smtp.exchange.umkc.edu [134.193.44.10]) 8, 26 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m6NIRx1k006352 8, 26 -- for } {microscopy-at-msa.microscopy.com} ; Wed, 23 Jul 2008 13:27:59 } -0500 8, 26 -- X-Received-From-Address: 134.193.32.11 8, 26 } -- X-Envelope-From: DusevichV-at-umkc.edu 8, 26 -- } X-Envelope-To: microscopy-at-msa.microscopy.com 8, 26 -- } Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by } kc-msxproto3.kc.umkc.edu with Microsoft } SMTPSVC(6.0.3790.3959); Wed, 23 Jul 2008 13:27:58 -0500 8, 26 } -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4133 } 8, 26 -- Importance: normal 8, 26 -- Priority: normal 8, 26 } -- Content-Class: urn:content-classes:message 8, 26 -- } MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- } charset="us-ascii" } 8, 26 -- Subject: } 8, 26 -- Date: Wed, 23 Jul 2008 13:27:58 -0500 8, 26 -- } Message-ID: } {032EC4F75A527A4FA58C5B1B5DECFBB33ADDC6-at-KC-MSX1.kc.umkc.edu} } 8, 26 -- X-MS-Has-Attach: } 8, 26 -- X-MS-TNEF-Correlator: } 8, 26 -- thread-index: Acjs8dToHbrh58NDTjucpUD38lpadA== 8, 26 } -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 8, 26 -- } To: {microscopy-at-msa.microscopy.com} 8, 26 -- } X-OriginalArrivalTime: 23 Jul 2008 18:27:58.0904 (UTC) } FILETIME=[D53F7B80:01C8ECF1] 8, 26 -- } Content-Transfer-Encoding: 8bit 8, 26 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m6NIRx1k006352 } ==============================End of - } Headers============================== } } } } }
==============================Original Headers============================== 7, 29 -- From DusevichV-at-umkc.edu Thu Jul 24 08:20:55 2008 7, 29 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6ODKtJM032556 7, 29 -- for {microscopy-at-msa.microscopy.com} ; Thu, 24 Jul 2008 08:20:55 -0500 7, 29 -- X-Envelope-From: DusevichV-at-umkc.edu 7, 29 -- X-Received-From-Address: 134.193.32.11 7, 29 -- X-Envelope-To: microscopy-at-msa.microscopy.com, nizets2-at-yahoo.com 7, 29 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 24 Jul 2008 08:20:55 -0500 7, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4133 7, 29 -- Content-Class: urn:content-classes:message 7, 29 -- MIME-Version: 1.0 7, 29 -- Importance: normal 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="iso-8859-1" 7, 29 -- Priority: normal 7, 29 -- Subject: RE: Scaffolds in OCT 7, 29 -- Date: Thu, 24 Jul 2008 08:20:54 -0500 7, 29 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB33ADDC9-at-KC-MSX1.kc.umkc.edu} 7, 29 -- In-Reply-To: {619443.34381.qm-at-web37405.mail.mud.yahoo.com} 7, 29 -- X-MS-Has-Attach: 7, 29 -- X-MS-TNEF-Correlator: 7, 29 -- Thread-Topic: Scaffolds in OCT 7, 29 -- thread-index: AcjtZs1Q93IvXB+XSD6+mvIRLmWVRAAKOc5g 7, 29 -- References: {619443.34381.qm-at-web37405.mail.mud.yahoo.com} 7, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 7, 29 -- To: "Stephane Nizet" {nizets2-at-yahoo.com} , {microscopy-at-msa.microscopy.com} 7, 29 -- X-OriginalArrivalTime: 24 Jul 2008 13:20:55.0032 (UTC) FILETIME=[1A2D7780:01C8ED90] 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6ODKtJM032556 ==============================End of - Headers==============================
Interesting story on NPR's Talk of the Nation yesterday about image manipulation, Photoshop, and believability. More focused on journalistic applications, but interesting none the less.
X-from: Nestor J. Zaluzec - Chair MSA Standards Subcommittee
Re: MSA Standards Subcommittee Meeting during MM2008 Topic: Open Meeting on Spectral Imaging File Formats
David, this has been covered more than once on this list, fairly recently. No, i am not doing the eye roll, just saying that if you don't get much response, search the archives. It is definitely within a year back.
Best regards, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Email: dlowry-at-asu.edu } Name: David Lowry } } Organization: Arizona State Universty } } Title-Subject: [Filtered] GFP antibody for immuno-TEM } } Question: can anyone recommend a good source for an anti-GFP antibody } for on-section labeling? Thank you, } } } Login Host: 149.169.133.114 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu Jul 24 08:16:50 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m6ODGmeD028855 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 24 Jul 2008 } 08:16:50 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240803c4ae312db8f7-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 24 Jul 2008 08:16:46 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: dlowry-at-asu.edu (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: GFP antibody for immuno-TEM } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 23 -- From vladislav_speransky-at-nih.gov Thu Jul 24 14:17:15 2008 5, 23 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6OJHEAL030020 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Jul 2008 14:17:15 -0500 5, 23 -- X-IronPortListener: NIH_Relay 5, 23 -- X-SBRS: None 5, 23 -- X-IronPort-AV: E=Sophos;i="4.31,248,1215403200"; 5, 23 -- d="scan'208";a="22637566" 5, 23 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 23 -- by nihrelayxway3.hub.nih.gov with ESMTP; 24 Jul 2008 15:17:12 -0400 5, 23 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6OJHBIC030054 5, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Jul 2008 15:17:12 -0400 5, 23 -- Mime-Version: 1.0 (Apple Message framework v753.1) 5, 23 -- References: {200807241318.m6ODIc5O030249-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 23 -- Message-Id: {EC5E609C-7240-4583-86F1-86A01F059430-at-nih.gov} 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 23 -- Subject: Fwd: [Microscopy] viaWWW: GFP antibody for immuno-TEM 5, 23 -- Date: Thu, 24 Jul 2008 15:16:10 -0400 5, 23 -- To: Microscopy-at-microscopy.com 5, 23 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
We have a Leica AFS cryo-substitution unit in our core. Like all our other Leica equipment, the AFS has worked well for us for many years. However, it is currently having problem cooling. It only goes down to - 57 degree while the setting is - 97 degree. Has any one with a AFS had similar problem, and if yes, what was the cause in your case? Thanks in advance for any reply off-line.
Hong
Emory EM
==============================Original Headers============================== 4, 21 -- From hyi-at-emory.edu Thu Jul 24 21:11:18 2008 4, 21 -- Received: from QMTA03.westchester.pa.mail.comcast.net (qmta03.westchester.pa.mail.comcast.net [76.96.62.32]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6P2BIV1023602 4, 21 -- for {microscopy-at-microscopy.com} ; Thu, 24 Jul 2008 21:11:18 -0500 4, 21 -- Received: from OMTA12.westchester.pa.mail.comcast.net ([76.96.62.44]) 4, 21 -- by QMTA03.westchester.pa.mail.comcast.net with comcast 4, 21 -- id tsnF1Z02y0xGWP8532BHzY; Fri, 25 Jul 2008 02:11:17 +0000 4, 21 -- Received: from [71.236.24.22] ([71.236.24.22]) 4, 21 -- by OMTA12.westchester.pa.mail.comcast.net with comcast 4, 21 -- id u2BH1Z0050UcM8P3Y2BH2C; Fri, 25 Jul 2008 02:11:17 +0000 4, 21 -- X-Authority-Analysis: v=1.0 c=1 a=OiYl9EgEtzlYV7KySBgA:9 4, 21 -- a=dY07_CO425OdVy_HEpoA:9 a=ShqQEJZwpVClX7jFuxk6XWBb7pYA:4 a=9OHTkwyHC8cA:10 4, 21 -- Mime-Version: 1.0 (Apple Message framework v753) 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- Message-Id: {2D5C9CCA-6169-4D70-B7DC-DDAE5D628C62-at-emory.edu} 4, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 21 -- To: Microscopy-at-microscopy.com 4, 21 -- From: Hong Yi {hyi-at-emory.edu} 4, 21 -- Subject: Leica AFS 4, 21 -- Date: Thu, 24 Jul 2008 22:11:14 -0400 4, 21 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
If it was my own money I'd go for a V750 Pro*, nominally 6,400x9,600 dpi. Assuming your negatives are colour then the additional features of the V750 Pro version over the Epson V700 are worth having [Monaco EZ Color and Silverfast® AI 6 software package] plus even more usefully you get a better set of optics on the scanner. Technically you should 'colour correct' the scanning using colour targets (that Silverfast can supply for $100 or so) but I just use a light box and an 8x inspection eyepiece to view the original slide [for shadow detail/brightness** and colour correction].
I'm not a fan of Microtek, although others are, but to me the Epsons just seem to offer a better driver interface and superior ultimate scan resolution. The Nikon 35mm film scanners are also excellent but you have to go to £2,000 to get negative sizes up to 9x6cm (and no scanning to A4 for film and reflective scans - not even to 1/4 plate TEM negatives). However these cheaper £500 scanners are aimed at the home and small office market [i.e. those on a budget], so treat them carefully, and always use the film holders. A reprographics department will charge about £10 per film to scan using say a Hasselblad FlexTight, so if you have hundreds of films to scan over a few years the Epson V750 starts to make real financial sense (and it's a lot more convenient). You also really need Photoshop CS3 (or Serif PhotoPlus 11/X2 or Photoshop Elements 6, if you are poor) and a fast PC with 3-4Gb memory (if you need to do much photo-editing on the colour negatives - B&W TEM negatives need minimal post-scanning adjustment).
Like my microscopes, I keep my home scanner covered in a plastic sheet when not in use and it sits on a granite slab to help isolate it from vibrations (a very heavy Sainbury's black granite chopping board + four stick on rubber feet - cost £15).
I assume by slides you mean colour slide film (and not histology/glass slides). The Epson V750 pro will scan glass slides/histology but you need cardboard support along the edges to raise the slide and prevent scratching the glass platen (a disaster). The Epson scans to a resolution that’s just slightly better than a 1x Nikon objective on our large Nikon Eclipse TE2000U inverted microscope (and it's a lot less fiddly than setting up the microscope for the 1x). However, if you move to a 4x objective, and above, and the Nikon microscope clearly resolves a lot more detail over the scanner. The £2,000 Nikon SUPER COOLSCAN 9000 ED has an optional 3x1" glass slide/histology scanner holder and Zeiss make a dedicated 3x1" glass slide scanner [Mirax Scan] that I assume is quite expensive. The Epson 750 pro will scan glass slides 'up to A4' in size (i.e. isn't limited to standard 3x1") - but it's only suitable for low res views of entire sections, you still need the photo-microscope for detailed morphology images.
I attach my article on 'film scanning on a budget' that goes into the principles of scanning film in more detail.
Regards
Keith
* see details and excellent independent review at http://www.photo-i.co.uk/Reviews/interactive/Epson%20V750/page_1.htm
**use Photoshop's shadow/highlight tool to correct for this
--------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568
-----Original Message----- X-from: maloneyb-at-fiu.edu [mailto:maloneyb-at-fiu.edu] Sent: 23 July 2008 17:50 To: kjmorris-at-well.ox.ac.uk
Dear Group: do you have any recommendations for a scanning all sorts of different size negatives - 35 mm film strip negatives, slides and EM negatives? Thanks in advance for any suggestions. Barb
==============================Original Headers============================== 1, 19 -- From maloneyb-at-fiu.edu Wed Jul 23 11:40:13 2008 1, 19 -- Received: from fmailhost05.isp.att.net (fmailhost05.isp.att.net [207.115.11.55]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6NGeDdt028363 1, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jul 2008 11:40:13 -0500 1, 19 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 19 -- by isp.att.net (frfwmhc05) with ESMTP 1, 19 -- id {20080723164012H0500ebrl7e} ; Wed, 23 Jul 2008 16:40:12 +0000 1, 19 -- X-Originating-IP: [74.166.189.137] 1, 19 -- Message-ID: {48875EC9.8030300-at-fiu.edu} 1, 19 -- Disposition-Notification-To: barbara maloney {maloneyb-at-fiu.edu} 1, 19 -- Date: Wed, 23 Jul 2008 12:39:37 -0400 1, 19 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 19 -- User-Agent: Thunderbird 2.0.0.14 (Windows/20080421) 1, 19 -- MIME-Version: 1.0 1, 19 -- To: Microscopy-at-microscopy.com 1, 19 -- Subject: scanner for EM negatives 1, 19 -- X-Priority: 2 (High) 1, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 23, 21 -- From kjmorris-at-well.ox.ac.uk Mon Jul 28 04:59:44 2008 23, 21 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 23, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6S9xhqp009677 23, 21 -- for {Microscopy-at-Microscopy.Com} ; Mon, 28 Jul 2008 04:59:44 -0500 23, 21 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 23, 21 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 23, 21 -- id 1KNPW3-00074o-6G 23, 21 -- for Microscopy-at-Microscopy.Com; Mon, 28 Jul 2008 10:59:43 +0100 23, 21 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 23, 21 -- To: {Microscopy-at-Microscopy.Com} 23, 21 -- Subject: FW: [Microscopy] scanner for EM negatives 23, 21 -- Date: Mon, 28 Jul 2008 10:59:43 +0100 23, 21 -- Message-ID: {11BAAE21984648AC84E565FD4DC7764F-at-CytoWhizz} 23, 21 -- MIME-Version: 1.0 23, 21 -- Content-Type: text/plain; 23, 21 -- charset="iso-8859-1" 23, 21 -- X-Mailer: Microsoft Office Outlook 11 23, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 23, 21 -- Thread-Index: Acjs5Bpr2M59irjHTnW3FXYhkBnACwDq6AMgAAIzcrA= 23, 21 -- Content-Transfer-Encoding: 8bit 23, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6S9xhqp009677 ==============================End of - Headers==============================
The JEOL JSM-6300F SEM at the Center for Advanced Microscopy at Michigan State University needs a new home very soon. It is currently on service contract and is in use on a daily basis. We need to move it out in the next few weeks to make room for a new JEOL 7500F SEM. Contact me soon for specifics (flegler-at-msu.edu) if you are interested. Stanley L. Flegler, Director Center for Advanced Microscopy Michigan State University
==============================Original Headers============================== 2, 19 -- From flegler-at-msu.edu Mon Jul 28 12:46:14 2008 2, 19 -- Received: from sys30.mail.msu.edu (sys30.mail.msu.edu [35.9.75.130]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6SHkEni010225 2, 19 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 12:46:14 -0500 2, 19 -- Received: from [35.8.77.201] (helo=CAMRATINGEN.msu.edu) 2, 19 -- by sys30.mail.msu.edu with esmtpsa (Exim 4.63 #1) 2, 19 -- (TLSv1:RC4-SHA:128) 2, 19 -- id 1KNWnW-0000tv-ER 2, 19 -- for microscopy-at-microscopy.com; Mon, 28 Jul 2008 13:46:14 -0400 2, 19 -- Message-Id: {6.0.3.0.2.20080728133552.031ba390-at-mail.msu.edu} 2, 19 -- X-Sender: flegler-at-mail.msu.edu 2, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.0.3.0 2, 19 -- Date: Mon, 28 Jul 2008 13:46:14 -0400 2, 19 -- To: microscopy-at-microscopy.com 2, 19 -- From: "Stanley L. Flegler" {flegler-at-msu.edu} 2, 19 -- Subject: SEM: Good SEM needs a new home 2, 19 -- Mime-Version: 1.0 2, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 19 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Does anyone have a current source for Canadian Balsam? I have someone who wants to use it as a ground for instrument varnish and is hoping a microscopist might have a lead on a good source.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 3, 29 -- From dsherman-at-purdue.edu Mon Jul 28 13:08:24 2008 3, 29 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6SI8OmV023998 3, 29 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 13:08:24 -0500 3, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 3, 29 -- by mailhub129.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m6SI8MiB029145 3, 29 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 14:08:22 -0400 3, 29 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 3, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m6SI8KFq020010 3, 29 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 14:08:22 -0400 3, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 3, 29 -- Mon, 28 Jul 2008 14:07:52 -0400 3, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 3, 29 -- Mon, 28 Jul 2008 18:05:01 +0000 3, 29 -- User-Agent: Microsoft-Entourage/12.11.0.080522 3, 29 -- Date: Mon, 28 Jul 2008 14:04:58 -0400 3, 29 -- Subject: Canadian Balsam 3, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 3, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {C4B3828A.30DFC%dsherman-at-purdue.edu} 3, 29 -- Thread-Topic: Canadian Balsam 3, 29 -- Thread-Index: Acjw3HI7GnZjRyfyTPmJq57LoALJ1Q== 3, 29 -- Mime-version: 1.0 3, 29 -- Content-type: text/plain; 3, 29 -- charset="US-ASCII" 3, 29 -- Content-transfer-encoding: 7bit 3, 29 -- X-OriginalArrivalTime: 28 Jul 2008 18:07:52.0358 (UTC) FILETIME=[DA27EC60:01C8F0DC] 3, 29 -- X-PMX-Version: 5.4.0.320885 3, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Dear Group - does anyone have an EM image of the following organisms: diatom: /Cyclotella choctawhatcheeana /dinoflagellate: /Prorocentrum hoffmanianum /Thanks Barb
==============================Original Headers============================== 1, 19 -- From maloneyb-at-fiu.edu Mon Jul 28 13:26:01 2008 1, 19 -- Received: from fmailhost03.isp.att.net (fmailhost03.isp.att.net [207.115.11.53]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6SIQ0ns004881 1, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 13:26:01 -0500 1, 19 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 19 -- by isp.att.net (frfwmhc03) with ESMTP 1, 19 -- id {20080728182600H0300pg2m9e} ; Mon, 28 Jul 2008 18:26:00 +0000 1, 19 -- X-Originating-IP: [74.166.189.137] 1, 19 -- Message-ID: {488E0F13.3030002-at-fiu.edu} 1, 19 -- Disposition-Notification-To: barbara maloney {maloneyb-at-fiu.edu} 1, 19 -- Date: Mon, 28 Jul 2008 14:25:23 -0400 1, 19 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 19 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 1, 19 -- MIME-Version: 1.0 1, 19 -- To: Microscopy-at-microscopy.com 1, 19 -- Subject: EM image 1, 19 -- X-Priority: 2 (High) 1, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Surplus Shed has some for $25 per quarter ounce of dry resin. The lot I got is very clean. The lot got is Optical quality as they claim. A 1/4 ounce is lot more resin that I thought it would be.
http://www.surplusshed.com/pages/item/b1077.html
An old link I have on science-info.net lead me to: http://store.studioproducts.com/Balsams-p-1-c-254.html
A short time spent using google with this search: site:SITE-TO-SERCH.com Balsam such as site:kruess.com Balsam founs Wards Scientific has it it as well.
http://wardsci.com/product.asp_Q_pn_E_IG0015071
Cutting and Pasting the SITE-TO-SERCH from list of sources such as these: http://micro.magnet.fsu.edu/primer/resources/services.html http://cmgm.stanford.edu/~footer/companies.html
Just using a mouse click to highlight the single term that needs to pasted over the old on it the Google search goes pretty fast if you have two Browser windows open side by side. If I were going to do a lot I would write a scrip to do it.
Best wishes Gordon
Gordon Couger science-info.net The leading source of free documentation on old scopes & collection of useful information on microscopy.
----- Original Message ---- X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} } } Does anyone have a current source for Canadian Balsam? I have someone who } wants to use it as a ground for instrument varnish and is hoping a } microscopist might have a lead on a good source. } } Debby } -- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 14, 16 -- From gcouger-at-science-info.net Mon Jul 28 17:45:27 2008 14, 16 -- Received: from web401.biz.mail.mud.yahoo.com (web401.biz.mail.mud.yahoo.com [68.142.201.32]) 14, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6SMjRLN026830 14, 16 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 17:45:27 -0500 14, 16 -- Received: (qmail 98160 invoked by uid 60001); 28 Jul 2008 22:45:27 -0000 14, 16 -- Received: from [66.103.115.181] by web401.biz.mail.mud.yahoo.com via HTTP; Mon, 28 Jul 2008 15:45:27 PDT 14, 16 -- X-Mailer: YahooMailRC/975.45 YahooMailWebService/0.7.199 14, 16 -- Date: Mon, 28 Jul 2008 15:45:27 -0700 (PDT) 14, 16 -- From: Gordon Couger {gcouger-at-science-info.net} 14, 16 -- Reply-To: Gordon Couger {gcouger-at-science-info.net} 14, 16 -- Subject: Re: [Microscopy] Canadian Balsam 14, 16 -- To: dsherman-at-purdue.edu 14, 16 -- Cc: microscopy-at-microscopy.com 14, 16 -- MIME-Version: 1.0 14, 16 -- Content-Type: text/plain; charset=us-ascii 14, 16 -- Message-ID: {406524.98155.qm-at-web401.biz.mail.mud.yahoo.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jinguo.wang-at-utdallas.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jinguo.wang-at-utdallas.edu Name: Jinguo Wang
Organization: University of Texas at Dallas
Title-Subject: [Filtered] Postdoctoral Position Open
Question: The materials science and engineering department in University of Texas at Dallas has a postdoctoral position open in the area of transmission electron microscopy of electronic materials and nanomaterials. The research project focuses on understanding structure and chemistry of electronic materials and in-situ TEM on the nanomaterials. Most of the research will be conducted on FEI dual beam FIB, JEOL 2100F Field Emission TEM/STEM equipped with an EDAX energy dispersive x-ray spectrometer (EDS), Gatan Enfina electron energy loss spectrometer (EELS), high-angle annular dark field STEM detector, and Nano-factory TEM-STM, TEM-AFM and TEM-nanoindentor holders. The ideal candidate for this position will have experience in SEM, FIB, HREM, STEM, EDS and EELS. The salary will be commensurate with qualifications and experience.
Please forward questions or send applications to:
Dr. Jinguo Wang Department of Materials Science Mail Station RL10 University of Texas at Dallas 800 W. Campbell Road Richardson, TX 75080-3021
Well, the lab at UCSC just bit the dust, so I have a new job starting in August.
After you all recover from visiting Albuquerque, how about some ideas for the classes I will be teaching to budding microscopists soon.
The three classes are 'Photography for the Lab Technician", 'Cell Ultrastructure", and 'Digital Imaging". Anything you think should be included, or not. These classes are for beginning students, so pretty basic stuff is the call.
I have some pretty good ideas, but wanted to check with the 'community'. After all, you might be hiring one of these guys/gals someday and I need to know what you want them to know.
Jon Krupp
==============================Original Headers============================== 6, 22 -- From jmkrupp-at-ucsc.edu Mon Jul 28 18:26:05 2008 6, 22 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6SNQ5QO021308 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 18:26:05 -0500 6, 22 -- Received: from [128.114.125.141] (HELO ucsc.edu) 6, 22 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 6, 22 -- with ESMTPS id 37397500 for microscopy-at-microscopy.com; Mon, 28 Jul 2008 16:26:05 -0700 6, 22 -- Received: by email-prod-be-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 6, 22 -- with PIPE id 54222959; Mon, 28 Jul 2008 16:26:05 -0700 6, 22 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 6, 22 -- Received: from [63.249.84.253] (account jmkrupp-at-ucsc.edu) 6, 22 -- by email-prod-be-2.ucsc.edu (CommuniGate Pro WEBUSER 5.1.11) 6, 22 -- with HTTP id 54222927 for microscopy-at-microscopy.com; Mon, 28 Jul 2008 16:25:51 -0700 6, 22 -- From: "Jonathan M Krupp" {jmkrupp-at-ucsc.edu} 6, 22 -- Subject: Course ideas 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- X-Mailer: CommuniGate Pro WebUser v5.1.11 6, 22 -- Date: Mon, 28 Jul 2008 16:25:51 -0700 6, 22 -- Message-ID: {web-54222927-at-email-prod-be-2.ucsc.edu} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain;charset=utf-8;format="flowed" 6, 22 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gxx-at-u.washington.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gxx-at-u.washington.edu Name: Xiaoxia
Title-Subject: [Filtered] How to prepare a TEM sample: nanoparticles with protein
Question: How to prepare a TEM sample: nanoparticles with protein I helped one user to study his nanoparticles. His nanoparticles were synthesized with protein, so the problem was that the nanoparticles were condensed together after protein solution dried. Any one has experience to deal with protein sample? Has this kind sample to be studied by Cryo-TEM or with Cryo holder? Thanks,
Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu -----Original Message----- X-from: jmkrupp-at-ucsc.edu [mailto:jmkrupp-at-ucsc.edu] Sent: Monday, July 28, 2008 7:29 PM To: Calomeni, Edward
Hi:
Well, the lab at UCSC just bit the dust, so I have a new job starting in August.
After you all recover from visiting Albuquerque, how about some ideas for the classes I will be teaching to budding microscopists soon.
The three classes are 'Photography for the Lab Technician", 'Cell Ultrastructure", and 'Digital Imaging". Anything you think should be included, or not. These classes are for beginning students, so pretty basic stuff is the call.
I have some pretty good ideas, but wanted to check with the 'community'. After all, you might be hiring one of these guys/gals someday and I need to know what you want them to know.
Jon Krupp
==============================Original Headers============================== 6, 22 -- From jmkrupp-at-ucsc.edu Mon Jul 28 18:26:05 2008 6, 22 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6SNQ5QO021308 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 18:26:05 -0500 6, 22 -- Received: from [128.114.125.141] (HELO ucsc.edu) 6, 22 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 6, 22 -- with ESMTPS id 37397500 for microscopy-at-microscopy.com; Mon, 28 Jul 2008 16:26:05 -0700 6, 22 -- Received: by email-prod-be-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 6, 22 -- with PIPE id 54222959; Mon, 28 Jul 2008 16:26:05 -0700 6, 22 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 6, 22 -- Received: from [63.249.84.253] (account jmkrupp-at-ucsc.edu) 6, 22 -- by email-prod-be-2.ucsc.edu (CommuniGate Pro WEBUSER 5.1.11) 6, 22 -- with HTTP id 54222927 for microscopy-at-microscopy.com; Mon, 28 Jul 2008 16:25:51 -0700 6, 22 -- From: "Jonathan M Krupp" {jmkrupp-at-ucsc.edu} 6, 22 -- Subject: Course ideas 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- X-Mailer: CommuniGate Pro WebUser v5.1.11 6, 22 -- Date: Mon, 28 Jul 2008 16:25:51 -0700 6, 22 -- Message-ID: {web-54222927-at-email-prod-be-2.ucsc.edu} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain;charset=utf-8;format="flowed" 6, 22 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 18, 36 -- From Edward.Calomeni-at-osumc.edu Tue Jul 29 07:31:45 2008 18, 36 -- Received: from TWAPP-VP01.OSUMC.EDU (pluto.osumc.edu [140.254.120.27]) 18, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TCViSQ007989 18, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 07:31:45 -0500 18, 36 -- Received: from [140.254.138.19] by TWAPP-VP01.OSUMC.EDU with ESMTP ( 18, 36 -- OSUMC **** SMTP Relay ****); Tue, 29 Jul 2008 08:31:36 -0400 18, 36 -- X-Server-Uuid: B9562FAF-5C56-437C-B754-33221F224476 18, 36 -- Received: from localhost (unknown [127.0.0.1]) by pfeg02.osumc.edu ( 18, 36 -- Postfix) with ESMTP id 6FAF738CB4 for {Microscopy-at-microscopy.com} ; Tue, 18, 36 -- 29 Jul 2008 12:31:36 +0000 (UTC) 18, 36 -- Received: from pfeg02.osumc.edu ([127.0.0.1]) by localhost (pfeg02 18, 36 -- [127.0.0.1]) (amavisd-new, port 10024) with LMTP id 00624-01-10 for 18, 36 -- {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 08:31:36 -0400 (EDT) 18, 36 -- Received: from msxc01.OSUMC.EDU (msxc01.osumc.edu [10.127.29.33]) by 18, 36 -- pfeg02.osumc.edu (Postfix) with ESMTP id 4D2AE38CA1 for 18, 36 -- {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 08:31:36 -0400 (EDT) 18, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 36 -- Content-class: urn:content-classes:message 18, 36 -- MIME-Version: 1.0 18, 36 -- Subject: RE: [Microscopy] Course ideas 18, 36 -- Date: Tue, 29 Jul 2008 08:31:35 -0400 18, 36 -- Message-ID: {7A541592F9A53A4E86E7A3D5C4C7F68C02493FD3-at-msxc01.OSUMC.EDU} 18, 36 -- In-Reply-To: {200807282328.m6SNSpgU027523-at-ns.microscopy.com} 18, 36 -- X-MS-Has-Attach: 18, 36 -- X-MS-TNEF-Correlator: 18, 36 -- Thread-Topic: [Microscopy] Course ideas 18, 36 -- Thread-Index: AcjxCbKi4W+lFGluRiOFvKn3h7LuWAAbJvGw 18, 36 -- References: {200807282328.m6SNSpgU027523-at-ns.microscopy.com} 18, 36 -- From: "Calomeni, Edward " {Edward.Calomeni-at-osumc.edu} 18, 36 -- To: Microscopy-at-microscopy.com 18, 36 -- X-Virus-Scanned: amavisd-new at osumc.edu 18, 36 -- X-WSS-ID: 6491D2221VG542359-01-01 18, 36 -- Content-Type: text/plain; 18, 36 -- charset=us-ascii 18, 36 -- Content-Transfer-Encoding: 8bit 18, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6TCViSQ007989 ==============================End of - Headers==============================
Jon: I currently teach a basic 200 level course in 'Scientific Imaging' at Hamilton College. It covers the basic principals of qualitative and quantitative digital imaging utilizing Adobe Photoshop, Image J, and Fovea Pro software. If you think it would be helpful I would be willing to send you the syllabus.
Ken Bart
On Jul 28, 2008, at 7:26 PM, jmkrupp-at-ucsc.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi: } } Well, the lab at UCSC just bit the dust, so I have a new } job starting in August. } } After you all recover from visiting Albuquerque, how about } some ideas for the classes I will be teaching to budding } microscopists soon. } } The three classes are 'Photography for the Lab } Technician", 'Cell Ultrastructure", and 'Digital Imaging". } Anything you think should be included, or not. These } classes are for beginning students, so pretty basic stuff } is the call. } } I have some pretty good ideas, but wanted to check with } the 'community'. After all, you might be hiring one of } these guys/gals someday and I need to know what you want } them to know. } } Jon Krupp } } ==============================Original } Headers============================== } 6, 22 -- From jmkrupp-at-ucsc.edu Mon Jul 28 18:26:05 2008 } 6, 22 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m6SNQ5QO021308 } 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 } 18:26:05 -0500 } 6, 22 -- Received: from [128.114.125.141] (HELO ucsc.edu) } 6, 22 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) } 6, 22 -- with ESMTPS id 37397500 for microscopy-at-microscopy.com; } Mon, 28 Jul 2008 16:26:05 -0700 } 6, 22 -- Received: by email-prod-be-2.ucsc.edu (CommuniGate Pro } PIPE 5.1.11) } 6, 22 -- with PIPE id 54222959; Mon, 28 Jul 2008 16:26:05 -0700 } 6, 22 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) } 6, 22 -- Received: from [63.249.84.253] (account jmkrupp-at-ucsc.edu) } 6, 22 -- by email-prod-be-2.ucsc.edu (CommuniGate Pro WEBUSER } 5.1.11) } 6, 22 -- with HTTP id 54222927 for microscopy-at-microscopy.com; } Mon, 28 Jul 2008 16:25:51 -0700 } 6, 22 -- From: "Jonathan M Krupp" {jmkrupp-at-ucsc.edu} } 6, 22 -- Subject: Course ideas } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- X-Mailer: CommuniGate Pro WebUser v5.1.11 } 6, 22 -- Date: Mon, 28 Jul 2008 16:25:51 -0700 } 6, 22 -- Message-ID: {web-54222927-at-email-prod-be-2.ucsc.edu} } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- Content-Type: text/plain;charset=utf-8;format="flowed" } 6, 22 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
Kenneth M. Bart Director, Microscopy & Imaging Facility Hamilton College Clinton, New York 13323 315.859.4715 kbart-at-hamilton.edu
==============================Original Headers============================== 9, 26 -- From kbart-at-hamilton.edu Tue Jul 29 07:47:52 2008 9, 26 -- Received: from mailer.hamilton.edu (mailer.hamilton.edu [150.209.8.97]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TClqn3021455 9, 26 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 07:47:52 -0500 9, 26 -- Received: from pmxchannel-daemon.mail.hamilton.edu by mail.hamilton.edu 9, 26 -- (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 2006)) 9, 26 -- id {0K4R00C13Q7S4C00-at-mail.hamilton.edu} for microscopy-at-microscopy.com; Tue, 9, 26 -- 29 Jul 2008 08:47:52 -0400 (EDT) 9, 26 -- Received: from [192.168.1.3] 9, 26 -- (cpe-69-202-148-239.twcny.res.rr.com [69.202.148.239]) 9, 26 -- by mail.hamilton.edu (Sun Java System Messaging Server 6.2-6.01 (built Apr 3 9, 26 -- 2006)) with ESMTPA id {0K4R00DTIQ7OCRE0-at-mail.hamilton.edu} for 9, 26 -- microscopy-at-microscopy.com; Tue, 29 Jul 2008 08:47:48 -0400 (EDT) 9, 26 -- Date: Tue, 29 Jul 2008 08:47:47 -0400 9, 26 -- From: "Kenneth M. Bart" {kbart-at-hamilton.edu} 9, 26 -- Subject: Re: [Microscopy] Course ideas 9, 26 -- In-reply-to: {200807282326.m6SNQmDH022945-at-ns.microscopy.com} 9, 26 -- To: microscopy-at-microscopy.com 9, 26 -- Message-id: {CA9F15D6-6F57-4B96-834B-FA53DE5BA44B-at-hamilton.edu} 9, 26 -- MIME-version: 1.0 9, 26 -- X-Mailer: Apple Mail (2.753.1) 9, 26 -- Content-type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 9, 26 -- Content-transfer-encoding: 7BIT 9, 26 -- X-PMX-Version: 5.2.2.285561, Antispam-Engine: 2.5.0.283055, 9, 26 -- Antispam-Data: 2008.7.29.123441 9, 26 -- References: {200807282326.m6SNQmDH022945-at-ns.microscopy.com} ==============================End of - Headers==============================
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Does anyone have a current source for Canadian Balsam? I have someone who wants to use it as a ground for instrument varnish and is hoping a microscopist might have a lead on a good source.
Debby
Hi,
Canada Balsam for microscopy is still readily availlable from several companies. The problem probably is to find a supplier who can deliver it.
Sources for Canada Balsam in Europe are:
Carl Roth "Canada balsam natural, Balsamum Canadense", for microscopy 25ml (ref. 8016.1), 100 ml (ref. 8016.2);
1) You can do some excellent imaging with a desktop optical scanner in reflective mode.
2) Try to emphasize "analysis", i.e., make a measurement. Size matters! So does shape.
3) Make a good reference list of books, journals, etc..... However, realize that most will just use Wikipedia. Hence, consider feeding their web desires, by making post their work on-line.
regards,
JQuinn
} From mail-at-ns.microscopy.com Mon Jul 28 19:22:00 2008 } Date: Mon, 28 Jul 2008 18:26:34 -0500 } To: jquinn-at-www.matscieng.sunysb.edu } From: jmkrupp-at-ucsc.edu } Reply-to: jmkrupp-at-ucsc.edu } X-Resent-From: "Microscopy Listserver" {microscopy-at-microscopy.com} } Subject: [Microscopy] Course ideas } Errors-To: MicroscopyListSpamFilter-at-microscopy.com } X-lewp: MicroscopyListSpam NAGS } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } Well, the lab at UCSC just bit the dust, so I have a new } job starting in August. } } After you all recover from visiting Albuquerque, how about } some ideas for the classes I will be teaching to budding } microscopists soon. } } The three classes are 'Photography for the Lab } Technician", 'Cell Ultrastructure", and 'Digital Imaging". } Anything you think should be included, or not. These } classes are for beginning students, so pretty basic stuff } is the call. } } I have some pretty good ideas, but wanted to check with } the 'community'. After all, you might be hiring one of } these guys/gals someday and I need to know what you want } them to know. } } Jon Krupp } } ==============================Original Headers============================== } 6, 22 -- From jmkrupp-at-ucsc.edu Mon Jul 28 18:26:05 2008 } 6, 22 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6SNQ5QO021308 } 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jul 2008 18:26:05 -0500 } 6, 22 -- Received: from [128.114.125.141] (HELO ucsc.edu) } 6, 22 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) } 6, 22 -- with ESMTPS id 37397500 for microscopy-at-microscopy.com; Mon, 28 Jul 2008 16:26:05 -0700 } 6, 22 -- Received: by email-prod-be-2.ucsc.edu (CommuniGate Pro PIPE 5.1.11) } 6, 22 -- with PIPE id 54222959; Mon, 28 Jul 2008 16:26:05 -0700 } 6, 22 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) } 6, 22 -- Received: from [63.249.84.253] (account jmkrupp-at-ucsc.edu) } 6, 22 -- by email-prod-be-2.ucsc.edu (CommuniGate Pro WEBUSER 5.1.11) } 6, 22 -- with HTTP id 54222927 for microscopy-at-microscopy.com; Mon, 28 Jul 2008 16:25:51 -0700 } 6, 22 -- From: "Jonathan M Krupp" {jmkrupp-at-ucsc.edu} } 6, 22 -- Subject: Course ideas } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- X-Mailer: CommuniGate Pro WebUser v5.1.11 } 6, 22 -- Date: Mon, 28 Jul 2008 16:25:51 -0700 } 6, 22 -- Message-ID: {web-54222927-at-email-prod-be-2.ucsc.edu} } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- Content-Type: text/plain;charset=utf-8;format="flowed" } 6, 22 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 12 -- From jquinn-at-www.matscieng.sunysb.edu Tue Jul 29 08:58:00 2008 12, 12 -- Received: from www.matscieng.sunysb.edu (www.matscieng.sunysb.edu [129.49.36.33]) 12, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TDw0hQ018373 12, 12 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 08:58:00 -0500 12, 12 -- Received: (from jquinn-at-localhost) 12, 12 -- by www.matscieng.sunysb.edu (8.11.6/8.11.6) id m6TDrI930146 12, 12 -- for microscopy-at-microscopy.com; Tue, 29 Jul 2008 09:53:18 -0400 12, 12 -- Date: Tue, 29 Jul 2008 09:53:18 -0400 12, 12 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu} 12, 12 -- Message-Id: {200807291353.m6TDrI930146-at-www.matscieng.sunysb.edu} 12, 12 -- To: microscopy-at-microscopy.com 12, 12 -- Subject: re: Course ideas ==============================End of - Headers==============================
COMPANY PROFILE: Energy Beam Sciences, Inc. has been serving the sciences, academia and industry since 1958 with the manufacturing and distribution of instruments, consumable items and software. Over this time we have developed an extensive list of high profile customers in the medical, materials and semiconductor markets, as well as many major Colleges and Universities. EBS has focused its energies on growth through our philosophy of developing a detailed understanding of our customers' needs and by being pro-active in offering solutions to help them excel. We strive to maintain a positive and high-energy work environment that encourages our team members to exercise their imagination and creativity and rewards them well for their success. We are committed to quality and consistency as demonstrated by our near term objective of achieving ISO 9001-2000 certification.
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POSITION DESCRIPTION: This management level position is for you if you are entrepreneurial, energetic and self motivated. You understand the operating principles of electron beam technology and EM imaging and have been successful in a sales environment. You are confident in your abilities and have the knowledge and desire necessary to take your career to the next level, leading the growth of an entire product line. Your primary responsibilities will be sales, marketing, and product development. You will work closely with our Engineers to develop new products for our EBeam Product Division. You will also work closely with Management to develop and execute short, medium and long range plans for this Business Unit. The Company will invest in your ideas and initiatives and your rewards will be based on your success.
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Michael R. Nesta Managing Director Energy Beam Sciences, Inc. Tel: 860 653-0411 Fax: 860 653-0422 MNesta-at-ebsciences.com www.ebsciences.com “ADDING BRILLIANCE TO YOUR VISION”
==============================Original Headers============================== 11, 20 -- From mls1-at-ebsciences.com Tue Jul 29 10:37:42 2008 11, 20 -- Received: from mail.ebsciences.com (mail.ebsciences.com [65.115.7.18]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TFbgUo005163 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 10:37:42 -0500 11, 20 -- Received: from oldmikenesta.ebsciences.private ([10.10.0.108]) 11, 20 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 11, 20 -- (Exim 4.69) 11, 20 -- (envelope-from {mls1-at-ebsciences.com} ) 11, 20 -- id 1KNrGf-0002fe-Nl 11, 20 -- for microscopy-at-microscopy.com; Tue, 29 Jul 2008 11:37:41 -0400 11, 20 -- Message-ID: {488F3944.70503-at-ebsciences.com} 11, 20 -- Date: Tue, 29 Jul 2008 11:37:40 -0400 11, 20 -- From: Mike Nesta {mls1-at-ebsciences.com} 11, 20 -- Organization: Energy Beam Sciences 11, 20 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 11, 20 -- MIME-Version: 1.0 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- Subject: Position Opening, EBeam Product Manager at Energy Beam Sciences 11, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I second the opinion that basic histology should be taught. I have found it very important in my work and it is difficult teaching TEM to young people who do not have an understanding of histology.
It was not a good idea to cut something so basic out of undergraduate biology curricula.
Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} ++
} From: {Edward.Calomeni-at-osumc.edu} } Reply-To: {Edward.Calomeni-at-osumc.edu} } Date: Tue, 29 Jul 2008 07:38:35 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] RE: Course ideas } } Jon, } } How about a course on basic mammalian histology. } } Edward P. Calomeni } Director EM Lab - Pathology } The Ohio State University } M018 Starling Loving Hall } 320 W. 10th Ave. } Columbus, OH 43210 } 614-293-5580 (office) } 614-293-8806 (lab) } edward.calomeni-at-osumc.edu } -----Original Message----- } X-from: jmkrupp-at-ucsc.edu [mailto:jmkrupp-at-ucsc.edu] } Sent: Monday, July 28, 2008 7:29 PM } To: Calomeni, Edward } Subject: [Microscopy] Course ideas
} Hi: } } Well, the lab at UCSC just bit the dust, so I have a new job starting in } August. } } After you all recover from visiting Albuquerque, how about some ideas for the } classes I will be teaching to budding microscopists soon. } } The three classes are 'Photography for the Lab Technician", 'Cell } Ultrastructure", and 'Digital Imaging". } Anything you think should be included, or not. These classes are for } beginning students, so pretty basic stuff is the call. } } I have some pretty good ideas, but wanted to check with the 'community'. } After all, you might be hiring one of these guys/gals someday and I need to } know what you want them to know. } } Jon Krupp
==============================Original Headers============================== 9, 27 -- From connellyps-at-nhlbi.nih.gov Tue Jul 29 11:05:03 2008 9, 27 -- Received: from nihxway6out.hub.nih.gov (nihxway6out.hub.nih.gov [128.231.90.114]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TG52cw019324 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 11:05:03 -0500 9, 27 -- X-IronPortListener: Outbound_SMTP 9, 27 -- Received: from nihcessmtp2.hub.nih.gov ([128.231.90.116]) 9, 27 -- by nihxway6out.hub.nih.gov with ESMTP; 29 Jul 2008 11:55:44 -0400 9, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 27 -- Tue, 29 Jul 2008 11:55:36 -0400 9, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.165]) with Microsoft Exchange Server HTTP-DAV ; 9, 27 -- Tue, 29 Jul 2008 15:55:36 +0000 9, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 27 -- Date: Tue, 29 Jul 2008 11:55:22 -0400 9, 27 -- Subject: Re: [Microscopy] RE: Course ideas 9, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 27 -- To: {Edward.Calomeni-at-osumc.edu} 9, 27 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 27 -- Message-ID: {C4B4B5AA.21D7%connellyps-at-nhlbi.nih.gov} 9, 27 -- Thread-Topic: [Microscopy] RE: Course ideas 9, 27 -- Thread-Index: Acjxk4HJwKDCAF2GEd203gANk2Yv1A== 9, 27 -- In-Reply-To: {200807291238.m6TCcZo9015765-at-ns.microscopy.com} 9, 27 -- Mime-version: 1.0 9, 27 -- Content-type: text/plain; 9, 27 -- charset="ISO-8859-1" 9, 27 -- X-OriginalArrivalTime: 29 Jul 2008 15:55:36.0770 (UTC) FILETIME=[8A970A20:01C8F193] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6TG52cw019324 ==============================End of - Headers==============================
I teach an undergraduate course in Microanatomy that includes vertebrate, mostly mammalian histology and basic light microscopy, and a course I call Biological Imaging, that concentrates on light and electron optics and digital imaging as applied to microscopy, using biological samples). I'd be happy to share syllabi.
Gary Radice University of Richmond
==============================Original Headers============================== 2, 21 -- From gradice-at-richmond.edu Tue Jul 29 11:38:27 2008 2, 21 -- Received: from nylon.richmond.edu (nylon.richmond.edu [141.166.30.20]) 2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TGcQ3X001056 2, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 11:38:27 -0500 2, 21 -- Received: from polyester.richmond.edu (polyester.richmond.edu [141.166.24.28]) 2, 21 -- by nylon.richmond.edu (8.13.1/8.13.1) with ESMTP id m6TGcQ8I011410 2, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 12:38:26 -0400 2, 21 -- Received: from ffa185175.richmond.edu ([10.70.0.215]) 2, 21 -- by polyester.richmond.edu (8.12.11.20060308/8.12.11) with ESMTP id m6TGcQw0029594 2, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 12:38:26 -0400 2, 21 -- Message-Id: {A2DBCFDB-BAC2-44A2-95F3-20E2332A9F0F-at-richmond.edu} 2, 21 -- From: gradice {gradice-at-richmond.edu} 2, 21 -- To: Microscopy-at-microscopy.com 2, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 21 -- Content-Transfer-Encoding: 7bit 2, 21 -- Mime-Version: 1.0 (Apple Message framework v926) 2, 21 -- Subject: [Microscopy] Course ideas 2, 21 -- Date: Tue, 29 Jul 2008 12:38:26 -0400 2, 21 -- X-Mailer: Apple Mail (2.926) 2, 21 -- X-Spam-Detail: -1.44 ALL_TRUSTED 2, 21 -- X-Scanned-By: MIMEDefang 2.63 on 141.166.24.28 ==============================End of - Headers==============================
The International NanoScience Community (http://www.nanopaprika.eu/) is a website founded on November 2007. It's similar to others community website, but this website is totally dedicated to the worldwide NanoScience community. You can share your stuff with the world here: photos, videos, art, thoughts, discussions, you name it, just make sure it's related to NanoScience.
András Paszternák is the creator and editor of The International NanoScience Community. András is a chemistry PhD student in Budapest, Hungary. The comunity's first target group was the "nano" researcher from Hungary. But he had already moved towards global network. The community currently has more than 850 members, researchers, students, industrial partners from Europe, India, USA and 50 others countries.
With the domain name nanopaprika.eu, (paprika is favorite spice / Hungary) the community is aiming into nanobio area. The community is fully equip with various functions like chat, scientific forum, 26 thematic groups (SPM, Nanotoxicity, etc.), photos, videos, e-shop, games and so on. Hence, it's definitely a good website for member to discuss, share infomation and develop ideas
The International NanoScience Community
-- András Paszternák creator and editor of The International NanoScience Community English - Web: http://www.nanopaprika.eu German - Web: http://www.nanopaprika.de E-mail: editor-at-nanopaprika.eu
==============================Original Headers============================== 8, 19 -- From andras.paszternak-at-nanopaprika.eu Tue Jul 29 12:15:19 2008 8, 19 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.179]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6THFJuS015457 8, 19 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 12:15:19 -0500 8, 19 -- Received: by py-out-1112.google.com with SMTP id f47so2454197pye.6 8, 19 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 10:15:19 -0700 (PDT) 8, 19 -- Received: by 10.141.88.3 with SMTP id q3mr3398292rvl.94.1217351718639; 8, 19 -- Tue, 29 Jul 2008 10:15:18 -0700 (PDT) 8, 19 -- Received: by 10.141.5.10 with HTTP; Tue, 29 Jul 2008 10:15:18 -0700 (PDT) 8, 19 -- Message-ID: {db6a8dd60807291015g12f12981laf40c1a870b6552-at-mail.gmail.com} 8, 19 -- Date: Tue, 29 Jul 2008 19:15:18 +0200 8, 19 -- From: "=?ISO-8859-1?Q?Andr=E1s_Pasztern=E1k?=" {andras.paszternak-at-nanopaprika.eu} 8, 19 -- To: microscopy-at-microscopy.com 8, 19 -- Subject: Mycroscopy users at The International NanoScience Community 8, 19 -- MIME-Version: 1.0 8, 19 -- Content-Type: text/plain; charset=ISO-8859-1 8, 19 -- Content-Disposition: inline 8, 19 -- Content-Transfer-Encoding: 8bit 8, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6THFJuS015457 ==============================End of - Headers==============================
I am working with someone who is interested in creating a portable/shippable TEM laboratory. They have research that they are doing in remote locations and the samples are not permitted out side of the countries. They are interested in purchasing a tabletop tissue processor, ultramicratome, knife breaker, and vacuum oven. I am currently using the Leica UC6 (and love it) and don't think I would recommend shipping it to remote locations on a regular basis. My suggestion would be to use the old MT-1 or MT-2. What do you think or does someone make a portable product already? Your help is appreciated! Sue
--
Sue Tyler Biologist JHT Inc. Contracting Cooperative Oxford Laboratory Center for Coastal Environmental Health & Biomolecular Research at Charleston (CCHEBR) USDOC/NOAA/NOS/NCCOS 904 South Morris St. Oxford, Maryland 21654-9724 410-226-5193 FAX: 410-226-5925 Email: Sue.Tyler-at-noaa.gov
==============================Original Headers============================== 5, 17 -- From Sue.Tyler-at-noaa.gov Tue Jul 29 13:25:55 2008 5, 17 -- Received: from mmp3.nems.noaa.gov (mmp3.nems.noaa.gov [140.90.121.158]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TIPtiL031570 5, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 13:25:55 -0500 5, 17 -- Received: from [10.60.12.198] by mmp3.nems.noaa.gov 5, 17 -- (Sun Java System Messaging Server 6.2-2.05 (built Apr 28 2005)) 5, 17 -- with ESMTPSA id {0K4S00H965V6GKE0-at-mmp3.nems.noaa.gov} for 5, 17 -- Microscopy-at-microscopy.com; Tue, 29 Jul 2008 14:25:54 -0400 (EDT) 5, 17 -- Date: Tue, 29 Jul 2008 14:25:54 -0400 5, 17 -- From: Sue Tyler {Sue.Tyler-at-noaa.gov} 5, 17 -- Subject: ultramicrotomes 5, 17 -- To: Microscopy-at-microscopy.com 5, 17 -- Message-id: {488F60B2.6040206-at-noaa.gov} 5, 17 -- MIME-version: 1.0 5, 17 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 5, 17 -- Content-transfer-encoding: 7BIT 5, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) ==============================End of - Headers==============================
At least. Throw in some plant, invert, and protist stuff... They need to know what they're looking at!
Aloha, Tina
} How about a course on basic mammalian histology.
} The three classes are 'Photography for the Lab Technician", 'Cell } Ultrastructure", and 'Digital Imaging". } Anything you think should be included, or not. These classes are for } beginning students, so pretty basic stuff is the call.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 21 -- From tina-at-pbrc.hawaii.edu Tue Jul 29 13:29:24 2008 6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TITNKD004655 6, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 13:29:24 -0500 6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m6TITJ4P014023 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 21 -- Tue, 29 Jul 2008 08:29:20 -1000 (HST) 6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m6TITIb6014020; 6, 21 -- Tue, 29 Jul 2008 08:29:19 -1000 (HST) 6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 21 -- Date: Tue, 29 Jul 2008 08:29:18 -1000 (HST) 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 21 -- X-Sender: tina-at-halia 6, 21 -- To: Edward.Calomeni-at-osumc.edu 6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] RE: Course ideas 6, 21 -- In-Reply-To: {200807291233.m6TCXEJ0009508-at-ns.microscopy.com} 6, 21 -- Message-ID: {Pine.GSO.4.21.0807290827460.14011-100000-at-halia} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I'm looking for a stain or stains that will differentiate phenol formaldehyde and para-aramid (Kevlar). The stain may be for light or electron microscopy.
Thanks in advance.
__________________ John W. Catino
Specialty Minerals, Inc. MINTEQ International, Inc. 640 N. 13th Street Easton, PA 18042
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==============================Original Headers============================== 9, 16 -- From John.Catino-at-Mineralstech.com Tue Jul 29 14:56:16 2008 9, 16 -- Received: from psmtp.com (exprod8ob118.obsmtp.com [64.18.3.35]) 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6TJuFYT028553 9, 16 -- for {Microscopy-at-Microscopy.com} ; Tue, 29 Jul 2008 14:56:16 -0500 9, 16 -- Received: from source ([65.209.12.89]) by exprod8ob118.postini.com ([64.18.7.12]) with SMTP; 9, 16 -- Tue, 29 Jul 2008 12:56:15 PDT 9, 16 -- Subject: Phenol Formaldehyde or Para-aramid Stains 9, 16 -- To: Microscopy-at-Microscopy.com 9, 16 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 9, 16 -- Message-ID: {OF4110060E.292BFB10-ON85257495.006D5E9B-85257495.006D84B5-at-mineralstech.com} 9, 16 -- From: John.Catino-at-Mineralstech.com 9, 16 -- Date: Tue, 29 Jul 2008 15:56:14 -0400 9, 16 -- X-MIMETrack: Serialize by Router on dpn1/Minerals_Tech(Release 6.5.4FP2|September 12, 2005) at 9, 16 -- 07/29/2008 03:56:16 PM 9, 16 -- MIME-Version: 1.0 9, 16 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
We have inherited a spiffy, like-new Miele G 7783 CD laboratory dishwasher. It doesn't magnify things for spit, but has the potential for keeping our labware nice and shiny.
Might anyone have a copy of the operating manual for this machine, or a link to a pdf for one? I have googled for it, checked the Miele website, and contacted their support folks, but so far, no good. Closest I can come is the 7883, which has more buttons and makes my head hurt.
Thanks much.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Tue Jul 29 16:23:10 2008 7, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6TLNAe8011628 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 16:23:10 -0500 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Tue, 29 Jul 2008 16:23:10 -0500 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="us-ascii" 7, 23 -- Subject: Keeping EM clean 7, 23 -- Date: Tue, 29 Jul 2008 16:23:10 -0500 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD77F0-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: Keeping EM clean 7, 23 -- thread-index: AcjxwUy/tQqEF+VJRlqop0VV72DNrA== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 29 Jul 2008 21:23:10.0073 (UTC) FILETIME=[4CDF1690:01C8F1C1] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6TLNAe8011628 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dennist-at-ascb.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dennist-at-ascb.org Name: Dave Ennist
Organization: American Society for Cell Biology
Title-Subject: [Filtered] Re: Course ideas
Question: All,
While we're on course ideas, the ASCB digital Image & Video Library is open to submissions from students (undergrads and grads) as well as faculty. Why not make submitting an image/video a requirement for biology microscopy lab courses?
Go to http://cellimages.ascb.org - follow the links to submit or go directly to the submission module - http://www.ascb.org/ivl/ The submission will be reviewed by the Editorial Board. Regards, Dave
David L. Ennist, PhD Director, Digital Resources The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 TEL: 301-347-9315 FAX: 301-347-9310 email: DEnnist-at-ascb.org website: http://cellimages.ascb.org/
Hi! I have been a teacher before working as a scientist. I have noticed that "pure" scientists are trained to talk to educated people, which make them concentrate on the content and much less on the way informations are presented. To wake up their interest, you cannot find better than talking about themselves! There was a discussion not too far ago on the same subject but in SEM. Someone gave an excellent list of "things" of everyday life which may awake interest (you never know :-)). Avoid protists of extreme artics but prefer invertebrates found in our beds. Also, it is always better for understanding and remembering when the informations can connect together. For example, presenting isolated histological slides is boring, but if you make the relationship with the function of the tissu presented, it all makes sense. Even better: you can present the structure and ask them to try to understand the function: why are there 2 external muscle layers in the small intestine? What function can have these bizarre globet cells? First to give the right answer can make a preparation of his own blood on a slide! ;-) (don't know the name in english, in french it is called "frottis sanguin") I can still remember (and it was 20 years ago) when our professor made us spit in a tube before centrifuging and showing us there were cells in there! What a surprise! It is important to present serious information in an entertaining way. Don't feed their brains, make them hungry! I imagine you want to present the phagocytosis and show what cellular structures are involved. Take "snapshots" of all steps of a macrophage digesting a bacteria and ask the pupils to sort them in the right order! Science is just (serious) fun! Stephane
I am looking for a small hot plate to use under a stereo microscope for mounting TEM specimens for ion milling, dimpling, etc. I've seen one before that had a 4x3 inch heating area and a frame around the outside that stayed cool enough to touch and only stood about an inch tall. I've done a lot of searching and can't find anything except a custom made one that was a bit pricey. If anyone knows where I can find such a hot plate, please let me know. Thanks.
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
-----Original Message----- X-from: cljohnson33-at-gmail.com [mailto:cljohnson33-at-gmail.com] Sent: Wednesday, July 30, 2008 9:22 AM To: White, N.W. (Woody)
Hello all,
I am looking for a small hot plate to use under a stereo microscope for mounting TEM specimens for ion milling, dimpling, etc. I've seen one before that had a 4x3 inch heating area and a frame around the outside that stayed cool enough to touch and only stood about an inch tall. I've done a lot of searching and can't find anything except a custom made one that was a bit pricey. If anyone knows where I can find such a hot plate, please let me know. Thanks.
Craig.
==============================Original Headers============================== 3, 34 -- From cljohnson33-at-gmail.com Wed Jul 30 08:15:36 2008 3, 34 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.173]) 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UDFa7j003890 3, 34 -- for {microscopy-at-microscopy.org} ; Wed, 30 Jul 2008 08:15:36 -0500 3, 34 -- Received: by ug-out-1314.google.com with SMTP id k40so234031ugc.40 3, 34 -- for {microscopy-at-microscopy.org} ; Wed, 30 Jul 2008 06:15:35 -0700 (PDT) 3, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 34 -- d=gmail.com; s=gamma; 3, 34 -- h=domainkey-signature:received:received:message-id:date:from:to 3, 34 -- :subject:mime-version:content-type:content-transfer-encoding 3, 34 -- :content-disposition; 3, 34 -- bh=95yRImwF1zBU+/i+WwepJjHHbsK06I2fL0oc/HaUYrI=; 3, 34 -- b=TY3QUufvJPVdY86/SldSuru3kfLZ79jRMb9GOflvaa7s7eKLnk2nlShCggyilKeDe0 3, 34 -- FFqDwenBpVDIhpdcsrztHvwS5EHxFKFnJWDZt9O7hSsAS6TQoCvNdw4f59ihfnh+2J7i 3, 34 -- Fhak8YDA5LDeoQHBFXQCqhx+UTLSQDf2JFVwc= 3, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 34 -- d=gmail.com; s=gamma; 3, 34 -- h=message-id:date:from:to:subject:mime-version:content-type 3, 34 -- :content-transfer-encoding:content-disposition; 3, 34 -- b=Di+psoK/Di5SK0JG3Nmjy5XM5ex+V5fzYbibIVwCvM7Uh85I2cnsklG0WbPYYd6CP7 3, 34 -- j8sZbvypAPEDOVu0f+s96i5Rc+v6F1URfipE6y1L1WjcF2k1WsnR9noHHpJJhAxLmy5r 3, 34 -- sriyKroFc/ZjNJVVXePFuTz27vvy9cyHkUCq8= 3, 34 -- Received: by 10.67.88.12 with SMTP id q12mr1248169ugl.30.1217423735300; 3, 34 -- Wed, 30 Jul 2008 06:15:35 -0700 (PDT) 3, 34 -- Received: by 10.67.90.15 with HTTP; Wed, 30 Jul 2008 06:15:35 -0700 (PDT) 3, 34 -- Message-ID: {611c9b6f0807300615k161650e3v37e6db9ee6f51c5-at-mail.gmail.com} 3, 34 -- Date: Wed, 30 Jul 2008 09:15:35 -0400 3, 34 -- From: "Craig Johnson" {cljohnson33-at-gmail.com} 3, 34 -- To: microscopy-at-microscopy.org 3, 34 -- Subject: Small Hot Plate for Spec Prep 3, 34 -- MIME-Version: 1.0 3, 34 -- Content-Type: text/plain; charset=ISO-8859-1 3, 34 -- Content-Transfer-Encoding: 7bit 3, 34 -- Content-Disposition: inline ==============================End of - Headers============================== ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Wed Jul 30 08:48:12 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UDmCN0018217 2, 28 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 08:48:12 -0500 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.10261971; 2, 28 -- Wed, 30 Jul 2008 09:46:31 -0400 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Wed, 30 Jul 2008 09:46:30 -0400 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- Subject: RE: [Microscopy] Small Hot Plate for Spec Prep 2, 28 -- Date: Wed, 30 Jul 2008 09:46:30 -0400 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87C22-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200807301322.m6UDM4gN015058-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] Small Hot Plate for Spec Prep 2, 28 -- Thread-Index: AcjyR1ncOvX/YPC/SSuxkmMzarWyBAAAo9Ow 2, 28 -- References: {200807301322.m6UDM4gN015058-at-ns.microscopy.com} 2, 28 -- To: {cljohnson33-at-gmail.com} , {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 30 Jul 2008 13:46:30.0581 (UTC) FILETIME=[ABEA2650:01C8F24A] 2, 28 -- From: "White, N.W. (Woody)" {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="us-ascii" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6UDmCN0018217 ==============================End of - Headers==============================
We construct our own hot plates for microscopy and other applications using flexible Kapton heaters from Watlow ( also available from others such as Cole Parmer) and applying them to the bottom surface of a 1/16" aluminum plate. A control thermocouple ( thin film TC from Omega Engineering) is applied to the top surface and the whole is potted in castable ceramic from Cotronics, Inc. with the center region of the aluminum exposed as a work surface. The height of the frame edge is up to the user. The total thickness of the heater plate with base insulation is about 15 mm. but can be made thinner for low temperature use. The heater is controlled by a PID controller ( we use a programmable ramp controller from Digi-Sense ). For isothermal use, after autotuning of the controller, the surface temperature cycles are less than 1 degree C but these exposed sufaces are sensitive to air movement. The Kapton heaters are good to 200 deg C but Watlow and others also make high temperature foil heaters capable of 590 deg C. If you use high temperatures you will need to avoid heat transfer to the objectives by using IR filters and possibly an air knife to reduce convection heating.
Be sure to observe good electrical safety, insulate all connections and connect the heater power ground line to the aluminum plate.
Regards,
James Carnahan
Edison Analytical Laboratories, Inc. 301 Nott Street Schenectady, NY 12305
(518) 393-2112
==============================Original Headers============================== 9, 22 -- From carnahan-at-edison-labs.com Wed Jul 30 08:54:52 2008 9, 22 -- Received: from mail3.dotsterhost.com (mail3.dotsterhost.com [72.5.54.189]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m6UDsp26028851 9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 08:54:52 -0500 9, 22 -- Received: (qmail 5381 invoked from network); 30 Jul 2008 13:54:51 -0000 9, 22 -- Received: from unknown (HELO Edisonlab) (carnahan-at-edison-labs.com-at-[72.92.161.220]) 9, 22 -- by 72.5.54.189 with SMTP; 30 Jul 2008 13:54:50 -0000 9, 22 -- Message-ID: {001501c8f24b$d44b1ae0$2e01a8c0-at-Edisonlab} 9, 22 -- From: "Jim Carnahan" {carnahan-at-edison-labs.com} 9, 22 -- To: {microscopy-at-microscopy.com} 9, 22 -- Subject: RE: small hot plate for stereoscope. 9, 22 -- Date: Wed, 30 Jul 2008 09:54:42 -0400 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; 9, 22 -- format=flowed; 9, 22 -- charset="iso-8859-1"; 9, 22 -- reply-type=original 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Priority: 3 9, 22 -- X-MSMail-Priority: Normal 9, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 9, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
If you want/need a DIY solution, have a look at Omega Engineering (http://www.omega.com/toc_asp/subsectionSC.asp?subsection=M01&book=Heaters). They have some self-adhesive heater pads that can be connected to a Variac, or used with a temperature sensor/controller. I have mounted one on the bottom of an aluminum plate that replaces the removable stage plate of the dissecting scope. There are lots of possibilities.
For working with transillumination, (for enrobing things in agarose) I took a length of the heating element from inside a "wire rope heater" (it's OK to lose the sheath, only a few volts was used...) and stuck to bottom of the glass stage plate with silicone caulk and ran with a controller. You may need to use a low voltage transformer if only a short piece of heater wire is used - will need to measure resistance/power and make choices.
Hope this helps,
Dale cljohnson33-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } } I am looking for a small hot plate to use under a stereo microscope for } mounting TEM specimens for ion milling, dimpling, etc. I've seen one } before that had a 4x3 inch heating area and a frame around the outside } that stayed cool enough to touch and only stood about an inch tall. } I've done a lot of searching and can't find anything except a custom } made one that was a bit pricey. If anyone knows where I can find such a } hot plate, please let me know. Thanks. } } Craig. } } ==============================Original Headers============================== } 3, 34 -- From cljohnson33-at-gmail.com Wed Jul 30 08:15:36 2008 } 3, 34 -- Received: from ug-out-1314.google.com (ug-out-1314.google.com [66.249.92.173]) } 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UDFa7j003890 } 3, 34 -- for {microscopy-at-microscopy.org} ; Wed, 30 Jul 2008 08:15:36 -0500 } 3, 34 -- Received: by ug-out-1314.google.com with SMTP id k40so234031ugc.40 } 3, 34 -- for {microscopy-at-microscopy.org} ; Wed, 30 Jul 2008 06:15:35 -0700 (PDT) } 3, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 3, 34 -- d=gmail.com; s=gamma; } 3, 34 -- h=domainkey-signature:received:received:message-id:date:from:to } 3, 34 -- :subject:mime-version:content-type:content-transfer-encoding } 3, 34 -- :content-disposition; } 3, 34 -- bh=95yRImwF1zBU+/i+WwepJjHHbsK06I2fL0oc/HaUYrI=; } 3, 34 -- b=TY3QUufvJPVdY86/SldSuru3kfLZ79jRMb9GOflvaa7s7eKLnk2nlShCggyilKeDe0 } 3, 34 -- FFqDwenBpVDIhpdcsrztHvwS5EHxFKFnJWDZt9O7hSsAS6TQoCvNdw4f59ihfnh+2J7i } 3, 34 -- Fhak8YDA5LDeoQHBFXQCqhx+UTLSQDf2JFVwc= } 3, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 3, 34 -- d=gmail.com; s=gamma; } 3, 34 -- h=message-id:date:from:to:subject:mime-version:content-type } 3, 34 -- :content-transfer-encoding:content-disposition; } 3, 34 -- b=Di+psoK/Di5SK0JG3Nmjy5XM5ex+V5fzYbibIVwCvM7Uh85I2cnsklG0WbPYYd6CP7 } 3, 34 -- j8sZbvypAPEDOVu0f+s96i5Rc+v6F1URfipE6y1L1WjcF2k1WsnR9noHHpJJhAxLmy5r } 3, 34 -- sriyKroFc/ZjNJVVXePFuTz27vvy9cyHkUCq8= } 3, 34 -- Received: by 10.67.88.12 with SMTP id q12mr1248169ugl.30.1217423735300; } 3, 34 -- Wed, 30 Jul 2008 06:15:35 -0700 (PDT) } 3, 34 -- Received: by 10.67.90.15 with HTTP; Wed, 30 Jul 2008 06:15:35 -0700 (PDT) } 3, 34 -- Message-ID: {611c9b6f0807300615k161650e3v37e6db9ee6f51c5-at-mail.gmail.com} } 3, 34 -- Date: Wed, 30 Jul 2008 09:15:35 -0400 } 3, 34 -- From: "Craig Johnson" {cljohnson33-at-gmail.com} } 3, 34 -- To: microscopy-at-microscopy.org } 3, 34 -- Subject: Small Hot Plate for Spec Prep } 3, 34 -- MIME-Version: 1.0 } 3, 34 -- Content-Type: text/plain; charset=ISO-8859-1 } 3, 34 -- Content-Transfer-Encoding: 7bit } 3, 34 -- Content-Disposition: inline } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 20 -- From dac-at-research.umass.edu Wed Jul 30 09:00:22 2008 5, 20 -- Received: from race3.oit.umass.edu (race3.oit.umass.edu [128.119.101.39]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UE0MlC010726 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 09:00:22 -0500 5, 20 -- Received: from [192.168.1.23] (66-189-7-169.dhcp.oxfr.ma.charter.com [66.189.7.169]) 5, 20 -- (authenticated bits=0) 5, 20 -- by race3.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m6UE0Lbh031253 5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 10:00:21 -0400 5, 20 -- Message-ID: {48908252.4040807-at-research.umass.edu} 5, 20 -- Date: Wed, 30 Jul 2008 10:01:38 -0500 5, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.16) Gecko/20080702 SeaMonkey/1.1.11 5, 20 -- MIME-Version: 1.0 5, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 5, 20 -- Subject: Re: [Microscopy] Small Hot Plate for Spec Prep 5, 20 -- References: {200807301320.m6UDKqlj012293-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200807301320.m6UDKqlj012293-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
For some reason I received your post directly from your account rather than from Microscopy. Regardless, you make a number of very useful statements. Essentially you argue that an instructor give students context for information and they learn better. Then you offer a metaphor that to me seems awkward.
On Jul 30, 2008, at 1:48 AM, nizets2-at-yahoo.com wrote:
} It is important to present serious information in an entertaining } way. Don't feed their brains, make them hungry!
I understand the "is the glass half full or half empty" metaphor. I appreciate statements like "sage on the stage". But I think, personally, that "don't feed their brains, make them hungry" misses the mark. I understand the direction of the metaphor, but I think it goes in a direction not appropriate. The following web site supports my concern.
Far too many students go to elementary school hungry everyday. Neither their brains nor their stomachs are sated. These individuals learn poorly and generally do not prosper in school. I know that I am literally interpreting your metaphor, which by the way I was not able to locate on the Net.
I do very much agree that students like talking about themselves, especially non-science majors. Provoking student discussion about themselves and the world in which they live is a highly useful instructional tool. However the approach has to be used very cautiously. In today's undergraduate classroom an instructor walks a very fine between depth and coverage. What would happen if a course in human anatomy spoke only of the GI tract, just the GI tract. That which was presented was in excellent depth, loaded with discussion, but only spoke of the alimentary system. Has the student had a human anatomy course? There was no mention of the musculoskeletal system. Can you have a introductory human anatomy course that deals only with the "pluck", using a vet path term? When these GI anatomy students are given an external exam on their content understanding of human anatomy, they will ace the GI section and not anything else.
These last comments take us down an interesting new road. Should introductory courses always be survey courses? How superficial should a survey course be? As a long-time instructor I know that students have easy and fast access to resources previously unthought of: Wikipedia etc. I can find many introductory courses already prepackaged on the web or on CD that accompany the textbook: testbanks, powerpoints, study guides, and primary literature pdf's. Does this mean the survey course is dead, or just modified to take advantage of this new access technology?
As I reflect back on my personal undergraduate learning career, those instructors that I remember best were not necessarily the most popular; they were the ones that provoked educational discipline in me. Continuing in a Piaget context, as I moved from concrete to formal thinking, these instructors guided me. Unfortunately and in my opinion, many students who take undergraduate introductory science courses are concrete thinkers in science and some are unwilling to make the commitment to transition to formal thinking in science because it may require both mathematical expression and logical (disciplined) expression. There is a more subtle concept to explore. Most high school students experience science in terms of physical science and biology and as such those disciplines at the high school level mean learning facts. Using human anatomy again, science means naming body parts. As such, those students who can assimilate information in this way think they are learning science. Stephane addesses this phenomenon by asking the students to consider the structure function relationships: why does the stomach have three muscle layers, as does the bladder, instead of two? I have my anatomy students explore the bifurcation angle of the trachea as a discussion point leading into fluid dynamics. Stephane also suggests a time honored biology learning approach of having the students explore their own body by doing their own "frottis sanguin" or blood smear.
To examine the other aspect of her quote: "it is important to present serious information in an entertaining way." For more than twenty years I have been haunted by Neal Postman's "Amusing ourselves to death." As a society we access our news from the colorful and entertaining USA today. TV newscasters are referred to as "talent". And people by the droves watch Entertainment Tonight and something that we call "reality programming." And now we have digital social constructs including facebook, myspace, and second life. All have high entertainment value; but, do they develop intellectual discipline?
Are we substituting entertainment for passion; are we substituting entertainment for enthusiasm? How many students think of teachers and professors as being epitomized by Ben Stein saying "Bueller, Bueller" as part of the class routine? Most evaluation systems of courses and professors discover that those profs who rate best with students are those that bring enthusiasm to class; but do not confuse entertainment with enthusiasm!
To conclude I don't like the entertaining metaphor: "Don't feed their brains, make them hungry!" But what would I substitute? Build their brains by demonstrating a hunger for knowledge.... not snappy enough. Create a hungry brain through enthusiasm. Not good enough. Spare the pun and spoil the metaphor. Oops not that. Challenge the brain by creating a hunger for knowledge. Well maybe.
Stephane, thanks for your thoughts and provoking me into action.
Bob Blystone
Robert V. Blystone, Ph.D. Professor of Biology Trinity University One Trinity Place San Antonio, Texas 78212 210-999-7243 FAX 210-999-7229 rblyston-at-trinity.edu www.trinity.edu/rblyston
I hope that you will be attending M&M 2008 in Albuquerque; I look forward to seeing many old friends and making new ones. Please stop by the “Richmond Booth†(the M&M 2009 Booth) in the registration area to learn of the wonderful opportunities this historic city has to offer next year, as well as to register for some great prizes.
See you soon, Sara Miller Local Arrangements Committee Member Past President, MSA, 2004
Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710
On Wed, Jul 30, 2008 at 11:46 AM, Justin Kraft {kraftpiano-at-gmail.com} wrote: } } (Disclaimer: This has hit one of my hot-button issues, education. I have been a passionate crusader of reform in science education for a while now, trying to put real science in the hands of younger students to give them a feel for the fact that the crux of science is discovery and wonder- not memorizing facts.) } } This discussion is beginning to remind me of when I was first given the standards to which I had to teach so that my students could pass a standardized test. } } When I design an introductory course, I try to allow at least 20% of the class time for additional material that will allow me to go in a direction that the students are hungering for. I understand that in a high school setting, I have a bit more luxury of time, but isn't there a way to design a course so that the material is a little more spread out? } } Recently, I was examining college chemistry curricula. The Chem-I class was loaded with information- more than I would have thought would be possible to cover in one semester. The chem-II course had about half as much material. It was more in depth, but not so much that the two courses couldn't be balanced a bit more. These two courses were meant to be taken as a two-semester chemistry introduction. } } All in all, I feel that as science instructors (I use this term to include all those who teach, not just high school or college, but technicians who work with students and tutors as well) we need to help change the course of science education to allow for more exploratory learning earlier on. All children are born scientists, but our educational system and methodology tend to train them not to be scientists, so much as buckets into which information is poured. The unfortunate part of training buckets is that once they've dumped the information on the test, there is little chance that they are able to put it back. (I know I'm stretching that analogy a little too far, but you get my point- it's the "Test it and forget it" mentality.) } } I believe that we should all start from the beginning and try to find ways to augment the science curriculum from the very start. Let's put elementary school students into basic labs and do demonstrations to "wow" them so we can keep their interest and wonder. Then, when they grow up, they will at least become good critical thinkers and have a healthy appreciation for science. } } While I don't necessarily agree with the tenet that we should present information for entertainment value, I think we should present information for enticement. Show them something and let them ask the questions about why- I think that was the crux of the "Don't feed, let the mind huger" analogy. When I taught physics, I would end the class before lab with a demonstration, and the students' homework was to think about the demo and write up a hypothesis about why/how it works. We should entice them to want to learn, not just give them information. } } (I'll climb down off my soap box now.) } } --Justin A. Kraft } } P.S. Please pardon any typos, I'm getting used to a new keyboard. } } On Wed, Jul 30, 2008 at 10:08 AM, {rblyston-at-trinity.edu} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Stephane: } } } } For some reason I received your post directly from your account } } rather than from Microscopy. Regardless, you make a number of very } } useful statements. Essentially you argue that an instructor give } } students context for information and they learn better. Then you } } offer a metaphor that to me seems awkward. } } } } On Jul 30, 2008, at 1:48 AM, nizets2-at-yahoo.com wrote: } } } } } It is important to present serious information in an entertaining } } } way. Don't feed their brains, make them hungry! } } } } } } I understand the "is the glass half full or half empty" metaphor. I } } appreciate statements like "sage on the stage". But I think, } } personally, that "don't feed their brains, make them hungry" misses } } the mark. I understand the direction of the metaphor, but I think it } } goes in a direction not appropriate. The following web site supports } } my concern. } } } } http://kennebecjournal.mainetoday.com/view/columns/4566365.html } } } } Far too many students go to elementary school hungry everyday. } } Neither their brains nor their stomachs are sated. These individuals } } learn poorly and generally do not prosper in school. I know that I } } am literally interpreting your metaphor, which by the way I was not } } able to locate on the Net. } } } } I do very much agree that students like talking about themselves, } } especially non-science majors. Provoking student discussion about } } themselves and the world in which they live is a highly useful } } instructional tool. However the approach has to be used very } } cautiously. In today's undergraduate classroom an instructor walks a } } very fine between depth and coverage. What would happen if a course } } in human anatomy spoke only of the GI tract, just the GI tract. That } } which was presented was in excellent depth, loaded with discussion, } } but only spoke of the alimentary system. Has the student had a human } } anatomy course? There was no mention of the musculoskeletal system. } } Can you have a introductory human anatomy course that deals only with } } the "pluck", using a vet path term? When these GI anatomy students } } are given an external exam on their content understanding of human } } anatomy, they will ace the GI section and not anything else. } } } } These last comments take us down an interesting new road. Should } } introductory courses always be survey courses? How superficial } } should a survey course be? As a long-time instructor I know that } } students have easy and fast access to resources previously unthought } } of: Wikipedia etc. I can find many introductory courses already } } prepackaged on the web or on CD that accompany the textbook: } } testbanks, powerpoints, study guides, and primary literature pdf's. } } Does this mean the survey course is dead, or just modified to take } } advantage of this new access technology? } } } } As I reflect back on my personal undergraduate learning career, those } } instructors that I remember best were not necessarily the most } } popular; they were the ones that provoked educational discipline in } } me. Continuing in a Piaget context, as I moved from concrete to } } formal thinking, these instructors guided me. Unfortunately and in } } my opinion, many students who take undergraduate introductory science } } courses are concrete thinkers in science and some are unwilling to } } make the commitment to transition to formal thinking in science } } because it may require both mathematical expression and logical } } (disciplined) expression. There is a more subtle concept to } } explore. Most high school students experience science in terms of } } physical science and biology and as such those disciplines at the } } high school level mean learning facts. Using human anatomy again, } } science means naming body parts. As such, those students who can } } assimilate information in this way think they are learning science. } } Stephane addesses this phenomenon by asking the students to consider } } the structure function relationships: why does the stomach have } } three muscle layers, as does the bladder, instead of two? I have my } } anatomy students explore the bifurcation angle of the trachea as a } } discussion point leading into fluid dynamics. Stephane also suggests } } a time honored biology learning approach of having the students } } explore their own body by doing their own "frottis sanguin" or blood } } smear. } } } } To examine the other aspect of her quote: "it is important to } } present serious information in an entertaining way." For more than } } twenty years I have been haunted by Neal Postman's "Amusing ourselves } } to death." As a society we access our news from the colorful and } } entertaining USA today. TV newscasters are referred to as "talent". } } And people by the droves watch Entertainment Tonight and something } } that we call "reality programming." And now we have digital social } } constructs including facebook, myspace, and second life. All have } } high entertainment value; but, do they develop intellectual discipline? } } } } Are we substituting entertainment for passion; are we substituting } } entertainment for enthusiasm? How many students think of teachers } } and professors as being epitomized by Ben Stein saying "Bueller, } } Bueller" as part of the class routine? Most evaluation systems of } } courses and professors discover that those profs who rate best with } } students are those that bring enthusiasm to class; but do not confuse } } entertainment with enthusiasm! } } } } To conclude I don't like the entertaining metaphor: "Don't feed } } their brains, make them hungry!" But what would I substitute? Build } } their brains by demonstrating a hunger for knowledge.... not snappy } } enough. Create a hungry brain through enthusiasm. Not good enough. } } Spare the pun and spoil the metaphor. Oops not that. Challenge the } } brain by creating a hunger for knowledge. Well maybe. } } } } Stephane, thanks for your thoughts and provoking me into action. } } } } Bob Blystone } } } } } } Robert V. Blystone, Ph.D. } } Professor of Biology } } Trinity University } } One Trinity Place } } San Antonio, Texas 78212 } } 210-999-7243 FAX 210-999-7229 } } rblyston-at-trinity.edu www.trinity.edu/rblyston } } } } } } ==============================Original Headers============================== } } 19, 19 -- From rblyston-at-trinity.edu Wed Jul 30 09:02:52 2008 } } 19, 19 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) } } 19, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UE2plG016003 } } 19, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 09:02:51 -0500 } } 19, 19 -- Received: from [192.168.0.2] (really [66.69.90.252]) } } 19, 19 -- by cdptpa-omta02.mail.rr.com with ESMTP } } 19, 19 -- id {20080730140249.BGYQ15817.cdptpa-omta02.mail.rr.com-at-[192.168.0.2]} ; } } 19, 19 -- Wed, 30 Jul 2008 14:02:49 +0000 } } 19, 19 -- Mime-Version: 1.0 (Apple Message framework v753.1) } } 19, 19 -- In-Reply-To: {200807300648.m6U6mIrc006511-at-ns.microscopy.com} } } 19, 19 -- References: {200807300648.m6U6mIrc006511-at-ns.microscopy.com} } } 19, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed } } 19, 19 -- Message-Id: {B795AA25-1561-48F3-AF2B-D1D4F46E7A70-at-trinity.edu} } } 19, 19 -- Content-Transfer-Encoding: 7bit } } 19, 19 -- From: Robert Blystone {rblyston-at-trinity.edu} } } 19, 19 -- Subject: Re: [Microscopy] Re: Course ideas } } 19, 19 -- Date: Wed, 30 Jul 2008 09:02:48 -0500 } } 19, 19 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com } } 19, 19 -- X-Mailer: Apple Mail (2.753.1) } } ==============================End of - Headers============================== } } } } -- } "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
If you can't find what you need already available for purchase you might consider making your own. For that purpose I recommend using the heating element for an automobile cigarette lighter (which should be available for less than $10 from an auto parts store) as the actual heating element. They are compact, operate on 12 volts, and should be easy to incorporate into whatever type of base you would like (for instance, you could easily have the base made of Macor machineable ceramic) -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Wed Jul 30 10:58:48 2008 1, 14 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UFwmL5004238 1, 14 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 10:58:48 -0500 1, 14 -- Received: FROM [76.241.186.164] (adsl-76-241-186-164.dsl.sfldmi.sbcglobal.net [76.241.186.164]) 1, 14 -- BY hackers.mr.itd.umich.edu ID 48908FB4.2F53F.30492 ; 1, 14 -- 30 Jul 2008 11:58:44 -0400 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240803c4b63eb30750-at-[76.241.186.164]} 1, 14 -- Date: Wed, 30 Jul 2008 11:58:41 -0400 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: small hot plate 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Fools rush in where angels fear to tread. I rushed in. Apology is offered for dwelling on a translation that I did not fully appreciate or comprehend. Again my apologies to Stephane and to those of you who gently corrected me, thank you.
Bob Blystone
Robert Blystone Professor of Biology Trinity University San Antonio, Texas 78212 rblyston-at-trinity.edu
==============================Original Headers============================== 11, 18 -- From rblyston-at-trinity.edu Wed Jul 30 11:23:38 2008 11, 18 -- Received: from goliad.its.trinity.edu (goliad.its.trinity.edu [131.194.151.124]) 11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UGNY61017944 11, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 11:23:38 -0500 11, 18 -- Received: from exchange.trinity.edu ([131.194.151.15]) by goliad.its.trinity.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 18 -- Wed, 30 Jul 2008 11:23:31 -0500 11, 18 -- Received: from [131.194.83.29] ([131.194.83.29]) by exchange.trinity.edu with Microsoft SMTPSVC(6.0.3790.3959); 11, 18 -- Wed, 30 Jul 2008 11:23:29 -0500 11, 18 -- Message-Id: {F642B1B3-2099-4996-AF51-5A336A3D8244-at-trinity.edu} 11, 18 -- From: Robert Blystone {rblyston-at-trinity.edu} 11, 18 -- To: microscopy-at-microscopy.com 11, 18 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 11, 18 -- Content-Transfer-Encoding: 7bit 11, 18 -- Mime-Version: 1.0 (Apple Message framework v926) 11, 18 -- Subject: Apologies 11, 18 -- Date: Wed, 30 Jul 2008 11:23:28 -0500 11, 18 -- X-Mailer: Apple Mail (2.926) 11, 18 -- X-OriginalArrivalTime: 30 Jul 2008 16:23:29.0872 (UTC) FILETIME=[9A3FE500:01C8F260] ==============================End of - Headers==============================
Technoorg-Linda makes a small hot plate that is used in their TEM sample preparation technique. It has a small hot surface as well as a heated 3 mm receptacle for TEM grids. Their unit is called the MicroHeat(TM) and it is available through South Bay Technology. Here is a link to see an image of the unit: http://www.southbaytech.com/shop/TL4.shtml
Woody White suggested a Mug warmer for the wax. Actually these are not hot enough for the wax most often used with sample preparation. A candle warmer tray is the same size and is warmer yet. It will significantly soften the wax, but it is not warm enough to get the wax to the temperature where it should flow for fixing your samples.
Disclaimer: South Bay Technology is the representative for Technoorg-Linda in the United States and sells the MicroHeat(TM) hotplate.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: cljohnson33-at-gmail.com [mailto:cljohnson33-at-gmail.com] Sent: Wednesday, July 30, 2008 6:19 AM To: Walck-at-SouthBayTech.com
Hello all,
I am looking for a small hot plate to use under a stereo microscope for mounting TEM specimens for ion milling, dimpling, etc. I've seen one before that had a 4x3 inch heating area and a frame around the outside that stayed cool enough to touch and only stood about an inch tall. I've done a lot of searching and can't find anything except a custom made one that was a bit pricey. If anyone knows where I can find such a hot plate, please let me know. Thanks.
Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.138 / Virus Database: 270.5.3/1564 - Release Date: 7/21/2008 6:42 AM
==============================Original Headers============================== 16, 22 -- From walck-at-southbaytech.com Wed Jul 30 12:43:22 2008 16, 22 -- Received: from nlpi101.prodigy.net (nlpi101.prodigy.net [207.115.36.117]) 16, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6UHhMlN002614 16, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 12:43:22 -0500 16, 22 -- X-ORBL: [99.154.21.201] 16, 22 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 16, 22 -- by nlpi101.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id m6UHhKjM016735; 16, 22 -- Wed, 30 Jul 2008 12:43:20 -0500 16, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 16, 22 -- To: {Microscopy-at-microscopy.com} 16, 22 -- Cc: {cljohnson33-at-gmail.com} 16, 22 -- Subject: RE: [Microscopy] Small Hot Plate for Spec Prep 16, 22 -- Date: Wed, 30 Jul 2008 10:41:26 -0700 16, 22 -- Message-ID: {002001c8f26b$7e0469a0$7801a8c0-at-dynamicbl8uno3} 16, 22 -- MIME-Version: 1.0 16, 22 -- Content-Type: text/plain; 16, 22 -- charset="us-ascii" 16, 22 -- Content-Transfer-Encoding: 7bit 16, 22 -- X-Mailer: Microsoft Office Outlook 11 16, 22 -- In-Reply-To: {200807301318.m6UDIvx7009014-at-ns.microscopy.com} 16, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 16, 22 -- Thread-Index: AcjyRtLmpObnK64tQNCV2rG26rsyxwAIu1MA ==============================End of - Headers==============================
I just came across it again, here's where I saw anti-GFP Ab used for EM very recently: GFP-tagged proteins visualized by freeze-fracture immuno-electron microscopy: a new tool in cellular and molecular medicine Horst Robeneka1*, Insa Buersa1,Oliver Hofnagela, Stefan Lorkowskia and Nicholas J. Seversb "This is an Accepted Work that has been peer-reviewed and approved for publication in the Journal of Cellular and Molecular Medicine but has yet to undergo copy-editing and proof correction." These folks used "rabbit polyclonal antibody raised against the entire sequence of GFP (ab290, abcam, Cambridge, UK)".
One more paper from my Mac's HD that must've been on my mind when I was saying there'd been recent mentions: T.H.Giddings in Journal of Microscopy, Vol. 212, Pt 1 October 2003, pp. 53–61. Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. There is a picture showing good IEM localization of a GFP construct on a plastic section. They are not telling which antibody was used, but/therefore you can ask them. I know they've been using anti-GFP successfully for IEM for a while in Boulder.
As you have probably guessed, I don't have a personal favorite ;). Only used an anti-GFP once, in 1999, didn't work, never tried again.
Vlad
} } Vlad, actually I did look at the archives and over the past 2.5 } years there have been only 2 suggestions for anti-GFP antibodies } from list queries, both from 2006. Nothing regarding sources for } antibodies from 2007 or 2008. Dave } } } } David Lowry } School of Life Sciences } Arizona State University } Tempe, AZ 85287-4501 } office: 480-965-3210 } lab: 480-965-2463 } } } } } } } -----Original Message----- } From: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] } Sent: Thu 7/24/2008 12:33 PM } To: David Lowry } Subject: [Microscopy] Fwd: viaWWW: GFP antibody for immuno-TEM } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } David, this has been covered more than once on this list, fairly } recently. No, i am not doing the eye roll, just saying that if you } don't get much response, search the archives. It is definitely within } a year back. } } Best regards, } Vlad } ________________________________________________ } Vlad Speransky, Staff Scientist } Supramolecular Structure and Function Resource } National Institute of Biomedical Imaging and Bioengineering, NIH } 13 South Dr, Rm. 3N17 MSC 5766 } Bethesda, MD 20892 } 301 496-3989 } vladislav_speransky-at-nih.gov } } } } Email: dlowry-at-asu.edu } } Name: David Lowry } } } } Organization: Arizona State Universty } } } } Title-Subject: [Filtered] GFP antibody for immuno-TEM } } } } Question: can anyone recommend a good source for an anti-GFP } antibody } } for on-section labeling? Thank you, } } } } } } Login Host: 149.169.133.114
==============================Original Headers============================== 8, 24 -- From vladislav_speransky-at-nih.gov Wed Jul 30 16:30:43 2008 8, 24 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov [128.231.90.98]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6ULUfkj026633 8, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 16:30:42 -0500 8, 24 -- X-IronPortListener: NIH_Relay 8, 24 -- X-SBRS: None 8, 24 -- X-IronPort-AV: E=Sophos;i="4.31,281,1215403200"; 8, 24 -- d="scan'208";a="205113685" 8, 24 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 8, 24 -- by nihrelayxway4.hub.nih.gov with ESMTP; 30 Jul 2008 17:30:39 -0400 8, 24 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 8, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6ULUc5c019202 8, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 17:30:39 -0400 8, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) 8, 24 -- References: {B9366E69DACF09458B402E4C4385012604B2ABD1-at-EX03.asurite.ad.asu.edu} 8, 24 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; format=flowed 8, 24 -- Message-Id: {99597AB7-843A-48FE-874E-FA102F929F39-at-nih.gov} 8, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 24 -- Subject: Fwd: [Microscopy] viaWWW: GFP antibody for immuno-TEM 8, 24 -- Date: Wed, 30 Jul 2008 17:29:33 -0400 8, 24 -- To: Microscopy-at-microscopy.com 8, 24 -- X-Mailer: Apple Mail (2.753.1) 8, 24 -- Content-Transfer-Encoding: 8bit 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6ULUfkj026633 ==============================End of - Headers==============================
I am not aware of any specially portable ultramicrotomes but wanted to chime in saying that the modern ones are fairly easy to set up and much less susceptible to environmental interference. If I had to choose, I would go with a modern RMC or Leica for portability over an MT, definitely (I did work on an MT-2 for a year, and on an LKB-III for 5 years). Besides RMC and Leica, I remember there used to be some more obscure offerings from MicroStar, aimed at low budget, but I know nothing about their portability or performance.
Instead of a knife-breaker, why not use a few diamond knives ("rough", "better", and "best", for example, for different stages of work)? Seems better than carrying around all that glass, plus the knife breaker, and making boats. Diamond knives have barely gone up in price in a long time.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} From: Sue.Tyler-at-noaa.gov } Date: July 29, 2008 2:26:44 PM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] ultramicrotomes } Reply-To: Sue.Tyler-at-noaa.gov } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I am working with someone who is interested in creating a } portable/shippable TEM laboratory. They have research that they are } doing in remote locations and the samples are not permitted out } side of } the countries. They are interested in purchasing a tabletop tissue } processor, ultramicratome, knife breaker, and vacuum oven. } I am currently using the Leica UC6 (and love it) and don't think I } would } recommend shipping it to remote locations on a regular basis. My } suggestion would be to use the old MT-1 or MT-2. What do you think or } does someone make a portable product already? } Your help is appreciated! } Sue } } -- } } } Sue Tyler } Biologist } JHT Inc. Contracting } Cooperative Oxford Laboratory } Center for Coastal Environmental Health } & Biomolecular Research at Charleston (CCHEBR) } USDOC/NOAA/NOS/NCCOS } 904 South Morris St. } Oxford, Maryland 21654-9724 } 410-226-5193 FAX: 410-226-5925 } Email: Sue.Tyler-at-noaa.gov } } } ==============================Original } Headers============================== } 5, 17 -- From Sue.Tyler-at-noaa.gov Tue Jul 29 13:25:55 2008 } 5, 17 -- Received: from mmp3.nems.noaa.gov (mmp3.nems.noaa.gov } [140.90.121.158]) } 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m6TIPtiL031570 } 5, 17 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Jul 2008 } 13:25:55 -0500 } 5, 17 -- Received: from [10.60.12.198] by mmp3.nems.noaa.gov } 5, 17 -- (Sun Java System Messaging Server 6.2-2.05 (built Apr 28 } 2005)) } 5, 17 -- with ESMTPSA id {0K4S00H965V6GKE0-at-mmp3.nems.noaa.gov} for } 5, 17 -- Microscopy-at-microscopy.com; Tue, 29 Jul 2008 14:25:54 } -0400 (EDT) } 5, 17 -- Date: Tue, 29 Jul 2008 14:25:54 -0400 } 5, 17 -- From: Sue Tyler {Sue.Tyler-at-noaa.gov} } 5, 17 -- Subject: ultramicrotomes } 5, 17 -- To: Microscopy-at-microscopy.com } 5, 17 -- Message-id: {488F60B2.6040206-at-noaa.gov} } 5, 17 -- MIME-version: 1.0 } 5, 17 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed } 5, 17 -- Content-transfer-encoding: 7BIT } 5, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) } ==============================End of - } Headers==============================
Across the road at the university, the main service lab. for the molecular folk routinely makes buckets of anti-GFP which is very good. I wonder if quite a few labs do this. Rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 02-6246 5475 GPO Box 1600 fax. 02-6246 5334 Canberra, ACT 2601
On 31/7/08 7:42 AM, "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } David, } } I just came across it again, here's where I saw anti-GFP Ab used for } EM very recently: } GFP-tagged proteins visualized by freeze-fracture immuno-electron } microscopy: a new tool in cellular and molecular medicine Horst } Robeneka1*, Insa Buersa1,Oliver Hofnagela, Stefan Lorkowskia and } Nicholas J. Seversb } "This is an Accepted Work that has been peer-reviewed and approved } for publication in the Journal of Cellular and Molecular Medicine but } has yet to undergo copy-editing and proof correction." } These folks used "rabbit polyclonal antibody raised against the } entire sequence of GFP (ab290, abcam, Cambridge, UK)". } } One more paper from my Mac's HD that must've been on my mind when I } was saying there'd been recent mentions: } T.H.Giddings in Journal of Microscopy, Vol. 212, Pt 1 October 2003, } pp. 53–61. } Freeze-substitution protocols for improved visualization of membranes } in high-pressure frozen samples. } There is a picture showing good IEM localization of a GFP construct } on a plastic section. They are not telling which antibody was used, } but/therefore you can ask them. I know they've been using anti-GFP } successfully for IEM for a while in Boulder. } } As you have probably guessed, I don't have a personal favorite ;). } Only used an anti-GFP once, in 1999, didn't work, never tried again. } } Vlad } } } } } } Vlad, actually I did look at the archives and over the past 2.5 } } years there have been only 2 suggestions for anti-GFP antibodies } } from list queries, both from 2006. Nothing regarding sources for } } antibodies from 2007 or 2008. Dave } } } } } } } } David Lowry } } School of Life Sciences } } Arizona State University } } Tempe, AZ 85287-4501 } } office: 480-965-3210 } } lab: 480-965-2463 } } } } } } } } } } } } } } -----Original Message----- } } From: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] } } Sent: Thu 7/24/2008 12:33 PM } } To: David Lowry } } Subject: [Microscopy] Fwd: viaWWW: GFP antibody for immuno-TEM } } } } } } } } } } ---------------------------------------------------------------------- } } ------ } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } } MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } } ------ } } } } David, this has been covered more than once on this list, fairly } } recently. No, i am not doing the eye roll, just saying that if you } } don't get much response, search the archives. It is definitely within } } a year back. } } } } Best regards, } } Vlad } } ________________________________________________ } } Vlad Speransky, Staff Scientist } } Supramolecular Structure and Function Resource } } National Institute of Biomedical Imaging and Bioengineering, NIH } } 13 South Dr, Rm. 3N17 MSC 5766 } } Bethesda, MD 20892 } } 301 496-3989 } } vladislav_speransky-at-nih.gov } } } } } } } Email: dlowry-at-asu.edu } } } Name: David Lowry } } } } } } Organization: Arizona State Universty } } } } } } Title-Subject: [Filtered] GFP antibody for immuno-TEM } } } } } } Question: can anyone recommend a good source for an anti-GFP } } antibody } } } for on-section labeling? Thank you, } } } } } } } } } Login Host: 149.169.133.114 } } } ==============================Original Headers============================== } 8, 24 -- From vladislav_speransky-at-nih.gov Wed Jul 30 16:30:43 2008 } 8, 24 -- Received: from nihrelayxway4.hub.nih.gov (nihrelayxway4.hub.nih.gov } [128.231.90.98]) } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m6ULUfkj026633 } 8, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 16:30:42 -0500 } 8, 24 -- X-IronPortListener: NIH_Relay } 8, 24 -- X-SBRS: None } 8, 24 -- X-IronPort-AV: E=Sophos;i="4.31,281,1215403200"; } 8, 24 -- d="scan'208";a="205113685" } 8, 24 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) } 8, 24 -- by nihrelayxway4.hub.nih.gov with ESMTP; 30 Jul 2008 17:30:39 -0400 } 8, 24 -- Received: from [128.231.217.77] (db477.niaid.nih.gov } [128.231.217.77]) } 8, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m6ULUc5c019202 } 8, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 17:30:39 -0400 } 8, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) } 8, 24 -- References: } {B9366E69DACF09458B402E4C4385012604B2ABD1-at-EX03.asurite.ad.asu.edu} } 8, 24 -- Content-Type: text/plain; charset=WINDOWS-1252; delsp=yes; } format=flowed } 8, 24 -- Message-Id: {99597AB7-843A-48FE-874E-FA102F929F39-at-nih.gov} } 8, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} } 8, 24 -- Subject: Fwd: [Microscopy] viaWWW: GFP antibody for immuno-TEM } 8, 24 -- Date: Wed, 30 Jul 2008 17:29:33 -0400 } 8, 24 -- To: Microscopy-at-microscopy.com } 8, 24 -- X-Mailer: Apple Mail (2.753.1) } 8, 24 -- Content-Transfer-Encoding: 8bit } 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m6ULUfkj026633 } ==============================End of - Headers==============================
Does anyone have a protocol for prep of mineralized bone? We want to preserve the minerals within the bone so decalcification is not an option.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 7, 29 -- From dsherman-at-purdue.edu Wed Jul 30 20:24:25 2008 7, 29 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6V1OP37008626 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 20:24:25 -0500 7, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 7, 29 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m6V1OOlf012485 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 21:24:24 -0400 7, 29 -- Received: from 1061exfe04a.itap.purdue.edu (1061exfe04a.itap.purdue.edu [128.210.1.11]) 7, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m6V1OOlt019477 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 21:24:24 -0400 7, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 29 -- Wed, 30 Jul 2008 21:23:49 -0400 7, 29 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 7, 29 -- Thu, 31 Jul 2008 01:23:49 +0000 7, 29 -- User-Agent: Microsoft-Entourage/12.11.0.080522 7, 29 -- Date: Wed, 30 Jul 2008 21:23:49 -0400 7, 29 -- Subject: TEM of mineralized bone 7, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 7, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 29 -- Message-ID: {C4B68C65.1F688%dsherman-at-purdue.edu} 7, 29 -- Thread-Topic: TEM of mineralized bone 7, 29 -- Thread-Index: AcjyrBWOQmXEyer/zUm27JgA1FxkJA== 7, 29 -- Mime-version: 1.0 7, 29 -- Content-type: text/plain; 7, 29 -- charset="US-ASCII" 7, 29 -- Content-transfer-encoding: 7bit 7, 29 -- X-OriginalArrivalTime: 31 Jul 2008 01:23:49.0807 (UTC) FILETIME=[160963F0:01C8F2AC] 7, 29 -- X-PMX-Version: 5.4.0.320885 7, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
On Wed, Jul 30, 2008 at 11:46 AM, Justin Kraft {kraftpiano-at-gmail.com} wrote: } } We should entice them to want to learn, not just give them information. } Hi!
This is all I wanted to say in the end ;-). Sorry for confusing "entertainment" and "enticement", these are the sort of subtleties that are hard to master when one uses a foreign language. It is good to be enthousiastic ourselves, as scientist or teacher, but it is a mistake to think that your own enthousiasm will suffice to entice your students (although it surely helps a lot). It is important to use the values of the kids, not yours! You don't want to force information in their brain! When I wanted to introduce the suject of cancer in my classroom, I used to ask the pupils if they knew someone who has/had cancer. Then I was suprised to see how the kids became interested by the subject, so much that I had to cool their enthousiasm down! A dialog is much more worth than a monolog.
Has anyone successfully used the Sigma G5402 anti goat IgG-gold antibody (rabbit) for TEM ICC? I am looking for this secondary for immediate delivery but need a very small quantity. Most suppliers either do not have it in stock or sell in 1ml quantities which is too much for our needs and correspondingly too expensive.
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 5, 29 -- From dsherman-at-purdue.edu Thu Jul 31 07:21:40 2008 5, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6VCLeRP026683 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jul 2008 07:21:40 -0500 5, 29 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 5, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m6VCLdax019072 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jul 2008 08:21:40 -0400 5, 29 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 5, 29 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m6VCLdQG002556 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jul 2008 08:21:39 -0400 5, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 29 -- Thu, 31 Jul 2008 08:21:38 -0400 5, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 5, 29 -- Thu, 31 Jul 2008 12:21:39 +0000 5, 29 -- User-Agent: Microsoft-Entourage/12.11.0.080522 5, 29 -- Date: Thu, 31 Jul 2008 08:21:38 -0400 5, 29 -- Subject: Anti-goat conjugate 5, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 5, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 5, 29 -- Message-ID: {C4B72692.31139%dsherman-at-purdue.edu} 5, 29 -- Thread-Topic: Anti-goat conjugate 5, 29 -- Thread-Index: AcjzB/rpW+hHLsuQSzWvsHqiui/7HQ== 5, 29 -- Mime-version: 1.0 5, 29 -- Content-type: text/plain; 5, 29 -- charset="US-ASCII" 5, 29 -- Content-transfer-encoding: 7bit 5, 29 -- X-OriginalArrivalTime: 31 Jul 2008 12:21:38.0907 (UTC) FILETIME=[FB741AB0:01C8F307] 5, 29 -- X-PMX-Version: 5.4.0.320885 5, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Dear Fellow Listservers, Thank you so much for your quick reply to my co-workers attempt to set up a mobile TEM lab. She has asked me to post another question about a duel tissue processor, I hope you can help.
I'd like to know if anyone is using a LYNX II Automated Tissue Processor for Histology and Electron Microscopy and if they find it useful for processing for resin embedding, ie LR White, Epon, Spurs. We have very thin tissue in our organism and some of the older literature uses resin based embedding media for high resolution/high magnification light microscopy.
--
Sue Tyler Biologist JHT Inc. Contracting Cooperative Oxford Laboratory Center for Coastal Environmental Health & Biomolecular Research at Charleston (CCHEBR) USDOC/NOAA/NOS/NCCOS 904 South Morris St. Oxford, Maryland 21654-9724 410-226-5193 FAX: 410-226-5925 Email: Sue.Tyler-at-noaa.gov
==============================Original Headers============================== 6, 17 -- From Sue.Tyler-at-noaa.gov Thu Jul 31 07:37:49 2008 6, 17 -- Received: from mmp1.nems.noaa.gov (mmp1.nems.noaa.gov [140.90.121.156]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6VCbmFl007849 6, 17 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2008 07:37:48 -0500 6, 17 -- Received: from [10.60.12.198] by mmp1.nems.noaa.gov 6, 17 -- (Sun Java System Messaging Server 6.2-2.05 (built Apr 28 2005)) 6, 17 -- with ESMTPSA id {0K4V00JUJF304Y90-at-mmp1.nems.noaa.gov} for 6, 17 -- Microscopy-at-microscopy.com; Thu, 31 Jul 2008 08:37:48 -0400 (EDT) 6, 17 -- Date: Thu, 31 Jul 2008 08:37:48 -0400 6, 17 -- From: Sue Tyler {Sue.Tyler-at-noaa.gov} 6, 17 -- Subject: More on portable TEM lab 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- Message-id: {4891B21C.4030705-at-noaa.gov} 6, 17 -- MIME-version: 1.0 6, 17 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 6, 17 -- Content-transfer-encoding: 7BIT 6, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) ==============================End of - Headers==============================
We have rebooted the system several times, and the message still being showed and we still unable to control the stage using the SEM graphical interface. We checked if anything is blocking the movement, and it is OK, we can manually move the stage using the knobs.
We also have checked all SEM's connects, and all seam to be OK.
As everybody said (Philips representative guys, you and Henrik), everything leads us to conclude that something was bumped while removing the Penning gauge. But while checking the cables, boards and connectors near the Penning, there is only one board with a couple of LEDs and white connectors, those could be bumped. The LEDs indicate PVP, HVP, AIR, VENT, ECONO.
The guys from FEI told us to check the stage power supply. Is this power supply near the penning?
Thanks for the help.
Regards, On Thu, 2008-07-31 at 11:11 -0400, Gerald Bourne wrote: } Filipi, } } SMCB is your stage motor controller board, and should have nothing to do } with cleaning the cold cathode gauge. You may have bumped something } when you removed the gauge. We sometimes see a similar error message } and it goes away with a re-boot. Make sure that nothing is obstructing } your stage movement, and that all connects are properly connected. Then } try to reboot the entire system. } } Jerry } } filipi-at-pucrs.br wrote: } } } } Hi folks, } } } } Does anyone in this list have a Philips XL30 SEM? } } } } We have toke off the penning for cleaning, and after put } } it back, we could not start up the SEM properly anymore. } } } } When starting up the server, it always stop at the } } stage initialization and show the following error: } } } } "SMCB driver - DirectSend: Put CMxx I/O bus hardware } } problem 2nd address (c128c01f)." } } } } We can make vacuum, and imaging. The only thing which } } doesn't work is the stage control. } } } } The question is: } } What the penning cleaning procedure has to do with the stage? } } } } We have been in contact with local Philips representative, } } and they told us this could be a cabling issue. } } } } We just want to figure out why the penning cleaning procedure } } could damage anything on stage control hardware. } } } } Regards, } } -- } } Filipi Vianna } } IDEIA/PUCRS Research and Development Institute } } +55 51 33203525 ext. 7794 } } http://www.pucrs.br/ideia } } -- Filipi Vianna IDEIA/PUCRS Research and Development Institute +55 51 33203525 ext. 7794 http://www.pucrs.br/ideia
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Email: jiang12-at-purdue.edu Name: Wen Jiang
Organization: Purdue University
Title-Subject: [Filtered] Electron Microscopist Position
Question: An electron microscopist position is immediately available in the Jiang group (http://jiang.bio.purdue.edu), Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. Purdue is well equipped with two FEI FEG cryo-microscopes (200kV and 300kV) and will install a new 300kV liquid He microscope in 2009. The applicant should be experienced in high resolution TEM imaging, and in specimen preparation and low dose cryo-EM imaging of biological samples. The duties will be mainly on cryo-EM imaging for research projects on viruses, large macromolecular complexes and nano-particles. The applicant should be able to work independently with strong capabilities in identifying and solving problems. The applicant should also be a team player that enjoys working in a collaborative environment. Please send CV, statement of cryo-EM research experiences and interests, and references to Dr. Wen Jiang (jiang12-at-purdue.edu).
Don't let the fact that the stage problem showed up when you cleaned the penning gauge, cloud your trouble shooting of the stage problem. It may be a mere coincidence that the stage problem appeared at that point in time. Focus on the stage control system itself, and what may be wrong with it.
Sorry, I am not familiar with your system, so I can't offer any further advise. I think you may find your problem sooner, if you do this, though.
Regards, Darrell
filipi-at-pucrs.br wrote on 07/31/2008 03:43:28 PM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jerry, } } We have rebooted the system several times, and the message still } being showed and we still unable to control the stage using the } SEM graphical interface. We checked if anything is blocking the } movement, and it is OK, we can manually move the stage using the } knobs. } } We also have checked all SEM's connects, and all seam to be OK. } } As everybody said (Philips representative guys, you and Henrik), } everything leads us to conclude that something was bumped while } removing the Penning gauge. But while checking the cables, boards } and connectors near the Penning, there is only one board with a } couple of LEDs and white connectors, those could be bumped. } The LEDs indicate PVP, HVP, AIR, VENT, ECONO. } } The guys from FEI told us to check the stage power supply. } Is this power supply near the penning? } } Thanks for the help. } } Regards, } On Thu, 2008-07-31 at 11:11 -0400, Gerald Bourne wrote: } } Filipi, } } } } SMCB is your stage motor controller board, and should have nothing to do } } with cleaning the cold cathode gauge. You may have bumped something } } when you removed the gauge. We sometimes see a similar error message } } and it goes away with a re-boot. Make sure that nothing is obstructing } } your stage movement, and that all connects are properly connected. Then } } try to reboot the entire system. } } } } Jerry } } } } filipi-at-pucrs.br wrote: } } } } } } Hi folks, } } } } } } Does anyone in this list have a Philips XL30 SEM? } } } } } } We have toke off the penning for cleaning, and after put } } } it back, we could not start up the SEM properly anymore. } } } } } } When starting up the server, it always stop at the } } } stage initialization and show the following error: } } } } } } "SMCB driver - DirectSend: Put CMxx I/O bus hardware } } } problem 2nd address (c128c01f)." } } } } } } We can make vacuum, and imaging. The only thing which } } } doesn't work is the stage control. } } } } } } The question is: } } } What the penning cleaning procedure has to do with the stage? } } } } } } We have been in contact with local Philips representative, } } } and they told us this could be a cabling issue. } } } } } } We just want to figure out why the penning cleaning procedure } } } could damage anything on stage control hardware. } } } } } } Regards, } } } -- } } } Filipi Vianna } } } IDEIA/PUCRS Research and Development Institute } } } +55 51 33203525 ext. 7794 } } } http://www.pucrs.br/ideia } } } } -- } Filipi Vianna } IDEIA/PUCRS Research and Development Institute } +55 51 33203525 ext. 7794 } http://www.pucrs.br/ideia } } } ==============================Original Headers============================== } 8, 20 -- From filipi-at-pucrs.br Thu Jul 31 14:42:36 2008 } 8, 20 -- Received: from rigel.pucrs.br (rigel.pucrs.br [201.54.140.13]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m6VJgYTE019423 } 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2008 14:42:36 -0500 } 8, 20 -- Received: from [10.30.46.17] (unknown [10.30.46.17]) } 8, 20 -- by rigel.pucrs.br (Postfix) with ESMTP id 16D7F263FA } 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Jul 2008 16:42: } 34 -0300 (BRT) } 8, 20 -- Subject: Re: [Microscopy] Phillips XL30 } 8, 20 -- From: Filipi Vianna {filipi-at-pucrs.br} } 8, 20 -- To: Microscopy {Microscopy-at-microscopy.com} } 8, 20 -- In-Reply-To: {4891D611.9050404-at-mse.ufl.edu} } 8, 20 -- References: {200807311505.m6VF56X3032240-at-ns.microscopy.com} } 8, 20 -- {4891D611.9050404-at-mse.ufl.edu} } 8, 20 -- Content-Type: text/plain; charset=UTF-8 } 8, 20 -- Organization: =?ISO-8859-1?Q?ID=C9IA-PUCRS?= } 8, 20 -- Date: Thu, 31 Jul 2008 16:42:44 -0300 } 8, 20 -- Message-Id: {1217533364.31030.43.camel-at-filipi-desktop} } 8, 20 -- Mime-Version: 1.0 } 8, 20 -- X-Mailer: Evolution 2.22.3.1 } 8, 20 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 30 -- From milesd-at-us.ibm.com Thu Jul 31 18:10:22 2008 8, 30 -- Received: from e5.ny.us.ibm.com (e5.ny.us.ibm.com [32.97.182.145]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m6VNAKHd021751 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jul 2008 18:10:21 -0500 8, 30 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 8, 30 -- by e5.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id m6VNAJDb006296 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jul 2008 19:10:19 -0400 8, 30 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 8, 30 -- by d01relay02.pok.ibm.com (8.13.8/8.13.8/NCO v9.0) with ESMTP id m6VNAJJs210480 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jul 2008 19:10:19 -0400 8, 30 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 8, 30 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id m6VNAJ98002501 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jul 2008 19:10:19 -0400 8, 30 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 8, 30 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id m6VNAJ1B002498 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jul 2008 19:10:19 -0400 8, 30 -- In-Reply-To: {200807311943.m6VJhSpH020456-at-ns.microscopy.com} 8, 30 -- To: Microscopy-at-Microscopy.Com 8, 30 -- MIME-Version: 1.0 8, 30 -- Subject: Re: [Microscopy] Re: Phillips XL30 8, 30 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 8, 30 -- Message-ID: {OFA592C21F.ABF526D9-ON85257497.007F26BC-85257497.007F46FF-at-us.ibm.com} 8, 30 -- From: Darrell Miles {milesd-at-us.ibm.com} 8, 30 -- Date: Thu, 31 Jul 2008 19:10:18 -0400 8, 30 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1|February 07, 2008) at 8, 30 -- 07/31/2008 19:10:20, 8, 30 -- Serialize complete at 07/31/2008 19:10:20 8, 30 -- Content-Type: text/plain; charset="ISO-8859-1" 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m6VNAKHd021751 ==============================End of - Headers==============================
After the good experience in other research fields the The International NanoScience Community open a new thematic group for SEM and TEM users in the field of NanoScience.
The group is a good place to show your resaerch; ask others; find new partners.
The use of TINC site is free, everybody is welcome!
http://www.nanopaprika.eu/group/sem
TINC staff
==============================Original Headers============================== 6, 18 -- From andras.paszternak-at-nanopaprika.eu Fri Aug 1 05:36:45 2008 6, 18 -- Received: from rv-out-0708.google.com (rv-out-0708.google.com [209.85.198.249]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m71AajL3023677 6, 18 -- for {microscopy-at-microscopy.com} ; Fri, 1 Aug 2008 05:36:45 -0500 6, 18 -- Received: by rv-out-0708.google.com with SMTP id k29so1066919rvb.30 6, 18 -- for {microscopy-at-microscopy.com} ; Fri, 01 Aug 2008 03:36:44 -0700 (PDT) 6, 18 -- Received: by 10.141.18.15 with SMTP id v15mr5842151rvi.296.1217587004685; 6, 18 -- Fri, 01 Aug 2008 03:36:44 -0700 (PDT) 6, 18 -- Received: by 10.141.5.10 with HTTP; Fri, 1 Aug 2008 03:36:44 -0700 (PDT) 6, 18 -- Message-ID: {db6a8dd60808010336s56a8a055k9f07611611dd142d-at-mail.gmail.com} 6, 18 -- Date: Fri, 1 Aug 2008 12:36:44 +0200 6, 18 -- From: "=?ISO-8859-1?Q?Andr=E1s_Pasztern=E1k?=" {andras.paszternak-at-nanopaprika.eu} 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- Subject: SEM and TEM in NanoScience 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=ISO-8859-1 6, 18 -- Content-Transfer-Encoding: 7bit 6, 18 -- Content-Disposition: inline ==============================End of - Headers==============================
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Email: jar-at-virginia.edu Name: Jan Redick
Organization: Univ of Virginia/Advanced Microscopy Facility
Title-Subject: [Filtered] staining problem
Question: We have encountered a problem with our staining procedure for thin sections of biological samples which have been fixed routinely, stained en bloc with uranyl acetate, and embedded in either EPON or Spurr resin. Our "routine" contrast staining procedure is a triple-stain as follows: 1)lead citrate 4 min, rinse, dry 2) uranyl acetate in 50% acetone, 15 min, rinse and 3) lead citrate again 4 min, rinse, dry. This has worked beautifully on all kinds of tissues, in all kinds of embeddings for the last 15 years or so. (In none of these cases were the tissues stained en bloc with UA prior to embedding.) What happens in tissues that were en bloc stained with UA is that there is some kind of reversal in the electron density of tissues: for example, structures that are usually dark/black such as heterochromatin, membranes, Z lines in muscle, and RBC's, are light or nearly white....and areas which are typically light, such as euchromatic regions of a nucleus, and I bands in muscle are now dark! Can anyone shed light on this??? We are perplexed. Thanks for any help anyone can give.
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Email: timsubscribe-at-yahoo.com Name: Tim Zhang
Title-Subject: [Filtered] used optical tables or legs
Question: Dear everyone,
We are looking for a used optical table to match the one we have now. If you have a used optical table (4 by 8 by 1 foot) or just table legs to sell or donate, please contact me. Thanks.
Tim Zhang
Dept of Physics, Boise State University 208-426-3713
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Email: dawgdeluxe-at-gmail.com Name: Farid Jalali
Organization: STTARR Innovation Facility- Toronto
Title-Subject: [Filtered] Cytospinning cells onto a coverslip rather than a slide
Question: Hello Group,
I'd like to know if anyone has had success cytopsinning cells onto a No. 1.5 coverslip rather than microscope slides? If so, could you kindly provide any details of how this might be done.
Hi Farid! I never did that but I would simply hold a coverslip on a slide (tape, nail polish) and spin as usual! BTW, what is a N1.5 coverslip? I always describe coverslips by their size (18x18) but I may be wrong. Regards, Stephane
----- Original Message ---- X-from: "dawgdeluxe-at-gmail.com" {dawgdeluxe-at-gmail.com} To: nizets2-at-yahoo.com Sent: Sunday, August 3, 2008 8:58:06 PM
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Email: dawgdeluxe-at-gmail.com Name: Farid Jalali
Organization: STTARR Innovation Facility- Toronto
Title-Subject: [Filtered] Cytospinning cells onto a coverslip rather than a slide
Question: Hello Group,
I'd like to know if anyone has had success cytopsinning cells onto a No. 1.5 coverslip rather than microscope slides? If so, could you kindly provide any details of how this might be done.
We encountered a persistent scratch problem when we're cutting resin blocks with our diamond knife. Although we did all standart cleaning procedures (by using its original cleaner with absolute ethanol), we couldn't solve it. Any comments?
Necat Yilmaz
==============================Original Headers============================== 4, 31 -- From nyilmaz-at-mersin.edu.tr Mon Aug 4 06:15:05 2008 4, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m74BF4iT023663 4, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Aug 2008 06:15:05 -0500 4, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 1705114061F 4, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Aug 2008 14:10:58 +0300 (EEST) 4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 4, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 4, 31 -- with ESMTP id vUhMUX19a5xP for {Microscopy-at-microscopy.com} ; 4, 31 -- Mon, 4 Aug 2008 14:10:27 +0300 (EEST) 4, 31 -- Received: from nejat1 (unknown [193.255.129.131]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id AA754140693 4, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Aug 2008 14:10:27 +0300 (EEST) 4, 31 -- Message-ID: {000701c8f623$4a56f440$6b01a8c0-at-nejat1} 4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 4, 31 -- Subject: Diamond knife problem 4, 31 -- Date: Mon, 4 Aug 2008 14:14:27 +0300 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; 4, 31 -- format=flowed; 4, 31 -- charset="iso-8859-9"; 4, 31 -- reply-type=original 4, 31 -- Content-Transfer-Encoding: 7bit 4, 31 -- X-Priority: 3 4, 31 -- X-MSMail-Priority: Normal 4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
you don't say how old your knife is or how heavily used. I am assuming that you are cutting biological samples as you mention resin.
Scratches do eventually appear in diamond cut sections especially if lots of different users use a single diamond for different materials. If the scratches have appeared over time it might just be normal wear and tear but it might also be gritty or hard samples or centrifuged pellets which can accumulate tiny fragments of glass or deposits that could damage a diamond when sectioned.
There are special cleaning fluids which you could try in case its just a persistent particle stuck to the knife edge, but you need to be careful because some solvents will attack the adhesive mount of the diamond. If you do use such a fluid make sure its for diamond knife cleaning and follow the instructions carefully.
My regime with diamonds has been to cut as far to the left on the knife as possible and if it eventually scratches my sections move to the right until they are undamaged or I run out of knife. This works best for wide cutting-edge diamonds and small block faces.
If all else fails you might have to consider re-sharpening, a new diamond or glass knives.
Hope this helps.
Malcolm
Malcolm Haswell e.m. unit School of HNSS University of Sunderland SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: nyilmaz-at-mersin.edu.tr
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Email: martimor-at-nmsu.edu Name: Marti
Title-Subject: [Filtered] RE: reuse antibodies
Question: Hello, I was wondering if you could please give me some pointers on reusing antibodies? I have an antibody that is a gift and in a very limited amount. I would like to run several slides sets with the same antibody solution made over a period of 4-5 days. Do you prepare your primary in the blocking solution with this? Do you add sodium azide, if so, how much? I would appreciate greatly any advice based on your IHC experiences. This will be used on cryo sections and immunofluorescence application.
Hi Necat, - Is the scratch always at the same place relative to the knife? If so treat the knife. - Is the scratch always at the same place relative to the block? If so the problem comes from the block. I have noticed that some irregularities in resin curing may cause scratches (with Epon). Cleaning the knife edge with styropor sticks dipped in alcohol should clean it pretty well. Usually it is my "last resort" and always worked. If you suspect the edge to be damaged, try to inspect it with a microscope (don't try to insert it in the TEM though :-D) Best regards, Stephane
----- Original Message ---- X-from: "malcolm.haswell-at-sunderland.ac.uk" {malcolm.haswell-at-sunderland.ac.uk} To: nizets2-at-yahoo.com Sent: Monday, August 4, 2008 2:27:05 PM
Necat
you don't say how old your knife is or how heavily used. I am assuming that you are cutting biological samples as you mention resin.
Scratches do eventually appear in diamond cut sections especially if lots of different users use a single diamond for different materials. If the scratches have appeared over time it might just be normal wear and tear but it might also be gritty or hard samples or centrifuged pellets which can accumulate tiny fragments of glass or deposits that could damage a diamond when sectioned.
There are special cleaning fluids which you could try in case its just a persistent particle stuck to the knife edge, but you need to be careful because some solvents will attack the adhesive mount of the diamond. If you do use such a fluid make sure its for diamond knife cleaning and follow the instructions carefully.
My regime with diamonds has been to cut as far to the left on the knife as possible and if it eventually scratches my sections move to the right until they are undamaged or I run out of knife. This works best for wide cutting-edge diamonds and small block faces.
If all else fails you might have to consider re-sharpening, a new diamond or glass knives.
Hope this helps.
Malcolm
Malcolm Haswell e.m. unit School of HNSS University of Sunderland SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: nyilmaz-at-mersin.edu.tr
Hi Marti! I have already seen antibodies reused several times for Western blotting, but I never heard somebody re-using them for IHC. I don't know why though, but there may be some rationale. However I can give you a trick to limit the volume used for the reaction. Usually people use a big drop (100-150µl) ON a slide for the incubation. I prepare a 30-50µl (30µl should do) drop on a parafilm and invert a coverslip on it. To wash you just use a syringe and infiltrate the washing buffer under the coverslip. It avoids having to move your sample and risking to damage it (or let it fall). Given the very limited volume present under the coverslip, you must incubate in a humid atmosphere!! (which is anyway always advised). This is for 18x18 coverslips. Best regards, Stephane
----- Original Message ---- X-from: "martimor-at-nmsu.edu" {martimor-at-nmsu.edu} To: nizets2-at-yahoo.com Sent: Monday, August 4, 2008 11:15:37 PM
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Email: martimor-at-nmsu.edu Name: Marti
Title-Subject: [Filtered] RE: reuse antibodies
Question: Hello, I was wondering if you could please give me some pointers on reusing antibodies? I have an antibody that is a gift and in a very limited amount. I would like to run several slides sets with the same antibody solution made over a period of 4-5 days. Do you prepare your primary in the blocking solution with this? Do you add sodium azide, if so, how much? I would appreciate greatly any advice based on your IHC experiences. This will be used on cryo sections and immunofluorescence application.
in my old lab we routinely re-used primary antibodies for IHC 1 or 2 times with overnight incubation times (normally in the fridge). Between the incubations you store the antibody solution in the fridge if you use it again during the next 24h, if not you put it in the freezer. We never used sodium azide in the antibody solution but our specimen were routinely stored in sodium azide before IHC so there may have been some residues in them (although we always washed them several times). But it does not work for all antibodies, you have to test it. And even for some of the antibodies that we routinely re-used it did not always work. You have to be really careful that you e.g. do not get particles from your specimen into your antibody solution if you rescue it from a sample after incubation. So you won`t re-use an antibody on a sample that is very important or rare, let`s say if the sample is the limited resource. If not the sample is the limited resource but the antibody (as eventually in Mortimer`s case): what do you loose by reusing the antibody, just try it. If both, sample and antibody, are limited you may ask the source of the antibody whether they have any experience in re-using it. In the end you have to decide whether you take the risk of eventually loosing a valuable sample.
Kind regards,
Christian
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Marti! } I have already seen antibodies reused several times for Western blotting, but I never heard somebody re-using them for IHC. } I don't know why though, but there may be some rationale. } However I can give you a trick to limit the volume used for the reaction. Usually people use a big drop (100-150µl) ON a slide for the incubation. } I prepare a 30-50µl (30µl should do) drop on a parafilm and invert a coverslip on it. To wash you just use a syringe and infiltrate the washing buffer under the coverslip. It avoids having to move your sample and risking to damage it (or let it fall). Given the very limited volume present under the coverslip, you must incubate in a humid atmosphere!! (which is anyway always advised). This is for 18x18 coverslips. } Best regards, } Stephane } } } } ----- Original Message ---- } X-from: "martimor-at-nmsu.edu" {martimor-at-nmsu.edu} } To: nizets2-at-yahoo.com } Sent: Monday, August 4, 2008 11:15:37 PM } Subject: [Microscopy] viaWWW: reuse antibodies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both martimor-at-nmsu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: martimor-at-nmsu.edu } Name: Marti } } Title-Subject: [Filtered] RE: reuse antibodies } } Question: Hello, } I was wondering if you could please give me some pointers on reusing } antibodies? I have an antibody that is a gift and in a very limited } amount. I would like to run several slides sets with the same } antibody solution made over a period of 4-5 days. Do you prepare } your primary in the blocking solution with this? Do you add sodium } azide, if so, how much? I would appreciate greatly any advice based } on your IHC experiences. This will be used on cryo sections and } immunofluorescence application. } } Thanks in advance. } } Regards, } } Marti } } Login Host: 141.214.17.5 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Mon Aug 4 16:10:49 2008 } 8, 11 -- Received: from [192.168.200.224] (msdvpn8.msd.anl.gov [130.202.238.72]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m74LAmCN009353 } 8, 11 -- for {microscopy-at-microscopy.com} ; Mon, 4 Aug 2008 16:10:49 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c4bd20c83748-at-[192.168.200.224]} } 8, 11 -- Date: Mon, 4 Aug 2008 16:10:48 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: martimor-at-nmsu.edu (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: reuse antibodies } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } } } } } } ==============================Original Headers============================== } 21, 21 -- From nizets2-at-yahoo.com Tue Aug 5 12:31:38 2008 } 21, 21 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) } 21, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m75HVaeQ029607 } 21, 21 -- for {microscopy-at-microscopy.com} ; Tue, 5 Aug 2008 12:31:37 -0500 } 21, 21 -- Received: (qmail 94051 invoked by uid 60001); 5 Aug 2008 14:27:20 -0000 } 21, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 21, 21 -- s=s1024; d=yahoo.com; } 21, 21 -- h=Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; } 21, 21 -- b=KoJL9fHiSBfasL/vqr9dX2/vNK69WVSCUnNecp863/RuFlaTbVMe/NfmfNEQzXVhQBmjOgfBbI4fjy6hCm0LVJlFIp3TdhWujnzzB7jbMRpfaCkfRRPiwUcb0Ru7p4JswLaCZeo/xAkg1uqu72chMm5tTVm3+glsporwWhAUOOs=; } 21, 21 -- Received: from [80.122.101.100] by web37407.mail.mud.yahoo.com via HTTP; Tue, 05 Aug 2008 07:27:20 PDT } 21, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218 } 21, 21 -- Date: Tue, 5 Aug 2008 07:27:20 -0700 (PDT) } 21, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 21, 21 -- Subject: Re: [Microscopy] viaWWW: reuse antibodies } 21, 21 -- To: martimor-at-nmsu.edu } 21, 21 -- Cc: microscopy-at-microscopy.com } 21, 21 -- MIME-Version: 1.0 } 21, 21 -- Content-Type: text/plain; charset=iso-8859-1 } 21, 21 -- Message-ID: {732516.93810.qm-at-web37407.mail.mud.yahoo.com} } 21, 21 -- Content-Transfer-Encoding: 8bit } 21, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m75HVaeQ029607 } ==============================End of - Headers============================== }
--
Christian Liebig, PhD
FILM - Facility for Imaging by Light Microscopy - Facility Assistant - Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK
Just to follow up on Stephane's idea - we also use the coverslip approach but we use the pre-made coverslips designed for this (e.g., "LifterSlips" but alternative versions available) available from most microscopy supply outlets. I routinely used the 22 x 30 mm coverslips which require 15.6 ul to cover the surface but their 18 x 18 mm coverslip only requires 7.6 ul. We incubate overnight in a humidity chamber and then add a drop of buffer to the edge of the coverslip for 5 sec and then rinse it off into a beaker with more buffer. We recover the coverslips and wash them for re-use so it is quite economical.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, August 05, 2008 12:33 PM To: Phillips, Thomas E.
Hi Marti! I have already seen antibodies reused several times for Western blotting, but I never heard somebody re-using them for IHC. I don't know why though, but there may be some rationale. However I can give you a trick to limit the volume used for the reaction. Usually people use a big drop (100-150µl) ON a slide for the incubation. I prepare a 30-50µl (30µl should do) drop on a parafilm and invert a coverslip on it. To wash you just use a syringe and infiltrate the washing buffer under the coverslip. It avoids having to move your sample and risking to damage it (or let it fall). Given the very limited volume present under the coverslip, you must incubate in a humid atmosphere!! (which is anyway always advised). This is for 18x18 coverslips. Best regards, Stephane
----- Original Message ---- X-from: "martimor-at-nmsu.edu" {martimor-at-nmsu.edu} To: nizets2-at-yahoo.com Sent: Monday, August 4, 2008 11:15:37 PM
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Email: martimor-at-nmsu.edu Name: Marti
Title-Subject: [Filtered] RE: reuse antibodies
Question: Hello, I was wondering if you could please give me some pointers on reusing antibodies? I have an antibody that is a gift and in a very limited amount. I would like to run several slides sets with the same antibody solution made over a period of 4-5 days. Do you prepare your primary in the blocking solution with this? Do you add sodium azide, if so, how much? I would appreciate greatly any advice based on your IHC experiences. This will be used on cryo sections and immunofluorescence application.
I am a graduate student at Texas A&M University with a question related to my research.
I am implanting depleted U - Zr (10 w/o Zr) disks with He and wanting to look at the microstructural results with TEM. My question is about the final preparation of my samples. I plan on cutting the samples into disks and dimpling them before implantation.
However, I can't decide between ion milling or electrochemical polishing for the final thinning before analysis. I also have had trouble finding a proven specific solution to electropolish in.
I would appreciate any opinions on what process I should use. Thanks.
Kathryn Wright Mews Texas A&M University Department of Nuclear Engineering kowkat-at-gmail.com
==============================Original Headers============================== 5, 34 -- From kowkat-at-gmail.com Tue Aug 5 17:44:53 2008 5, 34 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.158]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m75Mirik016952 5, 34 -- for {microscopy-at-microscopy.com} ; Tue, 5 Aug 2008 17:44:53 -0500 5, 34 -- Received: by fg-out-1718.google.com with SMTP id 19so1372986fgg.4 5, 34 -- for {microscopy-at-microscopy.com} ; Tue, 05 Aug 2008 15:44:52 -0700 (PDT) 5, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 34 -- d=gmail.com; s=gamma; 5, 34 -- h=domainkey-signature:received:received:message-id:date:from:to 5, 34 -- :subject:mime-version:content-type:content-transfer-encoding 5, 34 -- :content-disposition; 5, 34 -- bh=qT+gIYPmUHJdICIoN+iECDQwL7g7a61xwBjHmKag/UU=; 5, 34 -- b=kmU1qKAps4zlTVxq7IPzT8c24I1OfLVJripLaPbb3eknxQo0RM2jS7C0MKK+IOvaFR 5, 34 -- dkOVmPMltegYfJFxCIZnB405znEvUe08d51ytpP/2saQ/nZha9EHRH7PgVQpH8QL69h0 5, 34 -- rN8gMZ0YzvgcHPZeQYUiDFeSOUmIFZk6gojMU= 5, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 34 -- d=gmail.com; s=gamma; 5, 34 -- h=message-id:date:from:to:subject:mime-version:content-type 5, 34 -- :content-transfer-encoding:content-disposition; 5, 34 -- b=LIwhWPu3Zy/LJtijVOWtftv6khB4Vots9J7zt2fLYM6FuWuqxot/kidaw7PHto/jFg 5, 34 -- 4Z77zmNISnOTL9YLFBhTS8WkABb+9DKwWLfSEG/1F1nwu8zJ8x/c2m42Zk+M/X6jD8VK 5, 34 -- inesnhvlpasjBODC91aIBlCkq1jw6Zc0B5rrQ= 5, 34 -- Received: by 10.86.80.5 with SMTP id d5mr289976fgb.26.1217976292627; 5, 34 -- Tue, 05 Aug 2008 15:44:52 -0700 (PDT) 5, 34 -- Received: by 10.86.26.14 with HTTP; Tue, 5 Aug 2008 15:44:52 -0700 (PDT) 5, 34 -- Message-ID: {81e959c0808051544i27406791uc231d8a8e7894ec3-at-mail.gmail.com} 5, 34 -- Date: Tue, 5 Aug 2008 17:44:52 -0500 5, 34 -- From: "Kathryn Wright" {kowkat-at-gmail.com} 5, 34 -- To: microscopy-at-microscopy.com 5, 34 -- Subject: TEM Preparation of U-Zr 5, 34 -- MIME-Version: 1.0 5, 34 -- Content-Type: text/plain; charset=ISO-8859-1 5, 34 -- Content-Transfer-Encoding: 7bit 5, 34 -- Content-Disposition: inline ==============================End of - Headers==============================
Although it is possible to re-use diluted antibodies using some of the methods described n the list, we still must remember that the antibody suspension contains proteins with specific binding abilities (which we use to label our sections).
The consequence of using the diluted solution once is that all the best (quickest, most efficient binders) will stick to the sections (samples, tissues, etc). These are lost from the suspension that you save. Subsequent use will deplete other specific-binding proteins leaving eventually to a suspension of proteins that have no particular specificity. Basically, the sections are being used in the same way an affinity column is used to purify antibodies - all the good ones stick to the column for elution.
Although often a necessity for stretching out small amounts of precious antibody, it is generally not recommended to re-use them. Re-use has the potential to produce different labeling patterns each time so comparisons are not easily made between experiments. At the very least, if you do it, say so in the methods and results parts of the published work so that others can evaluate the final outcome.
Regards,
Paul Webster.
2100 W 3rd St Los Angeles, CA 90057.
"still in Albuquerque and having a great time in the exhibition hall"
-----Original Message----- X-from: martimor-at-nmsu.edu [mailto:martimor-at-nmsu.edu] Sent: Mon 8/4/2008 2:15 PM To: Webster, Paul
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both martimor-at-nmsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: martimor-at-nmsu.edu Name: Marti
Title-Subject: [Filtered] RE: reuse antibodies
Question: Hello, I was wondering if you could please give me some pointers on reusing antibodies? I have an antibody that is a gift and in a very limited amount. I would like to run several slides sets with the same antibody solution made over a period of 4-5 days. Do you prepare your primary in the blocking solution with this? Do you add sodium azide, if so, how much? I would appreciate greatly any advice based on your IHC experiences. This will be used on cryo sections and immunofluorescence application.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both MooreH-at-health.missouri.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: MooreH-at-health.missouri.edu Name: Harolod Moore
Organization: University of Missouri Health Sciences Center
Title-Subject: [Filtered] Tissue Processor
Question: Our Autotechnicon has developed a couple of problems and to buy replacement parts is cost prohibitative (we need a tiny 12 tooth gear for the clock motor). We are looking for a "parts" machine with a working 24 hour clock or a cheap working used instrument. Any help will be greatly appreciated.
Harold Moore Nephrology Rresearch Laboratories University of Missouri 3300 Lemone Ind. Blvd., DCI Bldg. Columbia, MO 65201 573-882-4433 MooreH-at-health.missouri.edu
Paul's statement is very relevant, certainly if working with dilute polyclonals or serum that was not previously affinity purified and where the relevant antibody fraction may sometimes be very small as Paul already mentioned. However, one might want to consider the following aspects: the affinity and concentration of the antibody used and to a lesser extent the number of antibodies bound on the section per incubation.
It is still quite common to express antibody incubation characteristics in terms of dilution, but a concentration would be a more workable alternative. Concentrations in the order of magnitude of 0.1 - 1 microgram per ml are quite current and comply with average affinity constants for equilibrium antigen/antibody interactions. Assuming a 0.1 microgram/ml working dilution and a 5 µl incubation droplet there are about 2x10^9 antibody molecules in that droplet. Part of those will be removed as a result of binding in the primary antibody incubation step. If these were all purified high affinity antibodies or monoclonals with identical affinities the situation could be more favorable for reuse. Assuming a million antibodies would be bound to the grid and detected (wouldn't it be nice!), from a theoretical point of view it changes the originally available amount by only 0.05% and for all practical purposes it seems the droplet can almost not be depleted.... unless I made a horrible math error.
So far, at least in theory, no reason not to give it a try.
Caveat: repeated use may be seriously limited by other factors. The diluted antibody may get "dirty" from handling, debris from sections, denaturation etc etc. And even more so, the droplet also gets diluted from carry over liquid, especially in such small volumes. But if it is the only option......
Regards, Jan ("Wish I was in Albuquerque!")
Aurion - President Research Advisor Costerweg 5 Honorary Research Fellow 6702 AA Wageningen Otago School of Medical Sciences The Netherlands Dunedin phone 31-317-497676 New Zealand fax 31-317-415955 www.aurion.nl ------------------------------------------------------------------------ -----------------
==============================Original Headers============================== 7, 18 -- From leunissen-at-aurion.nl Wed Aug 6 01:29:33 2008 7, 18 -- Received: from mta04.xtra.co.nz (mta04.xtra.co.nz [210.54.141.251]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m766TWeu016073 7, 18 -- for {microscopy-at-microscopy.com} ; Wed, 6 Aug 2008 01:29:33 -0500 7, 18 -- Received: from [192.168.1.50] (really [222.152.88.175]) by mta04.xtra.co.nz 7, 18 -- with ESMTP 7, 18 -- id {20080806062931.YGAI11725.mta04.xtra.co.nz-at-[192.168.1.50]} 7, 18 -- for {microscopy-at-microscopy.com} ; Wed, 6 Aug 2008 18:29:31 +1200 7, 18 -- Mime-Version: 1.0 (Apple Message framework v753.1) 7, 18 -- Message-Id: {A5307D7A-666F-431A-A328-D2311CDF9C53-at-aurion.nl} 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 7, 18 -- To: microscopy-at-microscopy.com 7, 18 -- From: Jan Leunissen {leunissen-at-aurion.nl} 7, 18 -- Subject: Re-use of antibodies 7, 18 -- Date: Wed, 6 Aug 2008 18:29:30 +1200 7, 18 -- X-Mailer: Apple Mail (2.753.1) 7, 18 -- Content-Transfer-Encoding: 8bit 7, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m766TWeu016073 ==============================End of - Headers==============================
We do a lot of immunocytochemistry on paraffin and resin embedded tissues but I have a current project that requires cryostat sections. We have done this before but I am trying to optimize my protocol. The tissues were fixed in 2% paraformaldehyde and infiltrated into 30% sucrose before freezing in O.C.T. We cut 10-20 micron sections and picked them up on aminopropyltriethoxysilane (APTS) coated slides. I have stored them at -80 C. The question concerns the next step. Some people use thaw and use immediately, others let them sit 30 min at room temperature and others 30 min at 37 C before staining - any thoughts on this step? Tom Phillips
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
We tried to image some cells on a light microscope in a 96 well plate with a 10x objective. The trouble is that we can not focus the cells because we can not come close enough to the plate due to the focus limitation of the microscope. If we slightly unscrew the objective we come quite close but it is just not enough. So I was wondering if there is a kind of adapter that I could screw into the nose piece and then screw the objective into the adapter. If so, where could I find something like this?
Thanks a lot
Pascal
==============================Original Headers============================== 6, 19 -- From Lorentz.Pascal-at-gmail.com Thu Aug 7 04:58:22 2008 6, 19 -- Received: from balu3.urz.unibas.ch (balu3.urz.unibas.ch [131.152.1.57]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m779wLHU005572 6, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Aug 2008 04:58:22 -0500 6, 19 -- Received: from [131.152.78.22] (bioopticspc1.matten.unibas.ch [131.152.78.22]) 6, 19 -- by balu3.urz.unibas.ch (8.13.1/8.13.1) with ESMTP id m779wKXs018140 6, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Aug 2008 11:58:20 +0200 6, 19 -- Message-ID: {489AC736.3020909-at-gmail.com} 6, 19 -- Date: Thu, 07 Aug 2008 11:58:14 +0200 6, 19 -- From: Pascal Lorentz {Lorentz.Pascal-at-gmail.com} 6, 19 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 6, 19 -- MIME-Version: 1.0 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- Subject: LM: Adapter for Objective 6, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit 6, 19 -- X-SMTP-Vilter-Version: 1.3.2 6, 19 -- X-Brightmail-Tracker: AAAAAQAAAAI= 6, 19 -- X-Whitelist: TRUE ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT
Title-Subject: [Filtered] Polaron E5000
Question: Dear all, I am calling out to folks with the Polaron E5000 gold sputter coater.
I changed the target last week and it hasn't worked since. I figured out that it was the coaxial cable going into the head manifold. So I changed it out with 75 ohm coax cable that I got in a regular shop.
So the system now pumps down fine and illustrates the required current (20mA) BUT ......no plasma. I know for sure that the Argon is working fine.
This is where my knowledge stops (my knowledge of transformers, resistors etc... is minimal).... so, if anyone has any suggestions as to why the plasma isn't present, I'll be glad to hear from you.
Also, is anyone aware of someone that services these instruments in Ireland (for a low price!) I'd also be glad to hear from you.
Thanks for you hints Maybe I have to mention that we are doing this on a inverted microscope. So in that case we are already imaging from the bottom of the plate. I know imaging trough the plastic is not the best thing. But anyway. And since this experiment is only done once in a while we don't want to buy a long working distance objective. That's why I'm looking for a solution with the present setup.
Cheers
Pascal
Andre Dufresne schrieb: } Hi Pascal, } } Just had a request similar ( however, I'm doing epi-fluorescence) to } what you are doing with the 96 well plate. I'm interested in the } results to your problem. Let me what transpires if you could. } } Cheers, } } André } On Thursday, August 7, 2008, at 05:01 AM, Lorentz.Pascal-at-gmail.com } wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Dear all } } } } We tried to image some cells on a light microscope in a 96 well plate } } with a 10x objective. } } The trouble is that we can not focus the cells because we can not come } } close enough to the plate due to the focus limitation of the microscope. } } If we slightly unscrew the objective we come quite close but it is just } } not enough. } } So I was wondering if there is a kind of adapter that I could screw into } } the nose piece and then screw the objective into the adapter. If so, } } where could I find something like this? } } } } Thanks a lot } } } } Pascal } } } } } } } } ==============================Original } } Headers============================== } } 6, 19 -- From Lorentz.Pascal-at-gmail.com Thu Aug 7 04:58:22 2008 } } 6, 19 -- Received: from balu3.urz.unibas.ch (balu3.urz.unibas.ch } } [131.152.1.57]) } } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m779wLHU005572 } } 6, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Aug 2008 } } 04:58:22 -0500 } } 6, 19 -- Received: from [131.152.78.22] } } (bioopticspc1.matten.unibas.ch [131.152.78.22]) } } 6, 19 -- by balu3.urz.unibas.ch (8.13.1/8.13.1) with ESMTP id } } m779wKXs018140 } } 6, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Aug 2008 } } 11:58:20 +0200 } } 6, 19 -- Message-ID: {489AC736.3020909-at-gmail.com} } } 6, 19 -- Date: Thu, 07 Aug 2008 11:58:14 +0200 } } 6, 19 -- From: Pascal Lorentz {Lorentz.Pascal-at-gmail.com} } } 6, 19 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) } } 6, 19 -- MIME-Version: 1.0 } } 6, 19 -- To: Microscopy-at-microscopy.com } } 6, 19 -- Subject: LM: Adapter for Objective } } 6, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } } 6, 19 -- Content-Transfer-Encoding: 7bit } } 6, 19 -- X-SMTP-Vilter-Version: 1.3.2 } } 6, 19 -- X-Brightmail-Tracker: AAAAAQAAAAI= } } 6, 19 -- X-Whitelist: TRUE } } ==============================End of - } } Headers============================== } } }
==============================Original Headers============================== 7, 21 -- From Lorentz.Pascal-at-gmail.com Thu Aug 7 10:10:40 2008 7, 21 -- Received: from balu3.urz.unibas.ch (balu3.urz.unibas.ch [131.152.1.57]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m77FAdw3010705 7, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Aug 2008 10:10:39 -0500 7, 21 -- Received: from [131.152.78.22] (bioopticspc1.matten.unibas.ch [131.152.78.22]) 7, 21 -- by balu3.urz.unibas.ch (8.13.1/8.13.1) with ESMTP id m77FAcft029644 7, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Aug 2008 17:10:39 +0200 7, 21 -- Message-ID: {489B1067.5040405-at-gmail.com} 7, 21 -- Date: Thu, 07 Aug 2008 17:10:31 +0200 7, 21 -- From: Pascal Lorentz {Lorentz.Pascal-at-gmail.com} 7, 21 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 7, 21 -- MIME-Version: 1.0 7, 21 -- To: Microscopy-at-microscopy.com 7, 21 -- Subject: Re: [Microscopy] LM: Adapter for Objective 7, 21 -- References: {22C4870C-648F-11DD-BB08-0050E440D427-at-ms.umanitoba.ca} 7, 21 -- In-Reply-To: {22C4870C-648F-11DD-BB08-0050E440D427-at-ms.umanitoba.ca} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- X-SMTP-Vilter-Version: 1.3.2 7, 21 -- X-Brightmail-Tracker: AAAAAQAAAAI= 7, 21 -- X-Whitelist: TRUE ==============================End of - Headers==============================
There are "long working distance" objectives available made by OEM and by others in the aftermarket to fit many different microscopes. The specs are generally listed on their web sites so you can determine if their objective would solve the problem before your purchase.
Take care, Bob
----- Original Message ----- X-from: {Lorentz.Pascal-at-gmail.com} To: {bobwcarter-at-gmail.com} Sent: Thursday, August 07, 2008 3:06 AM
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Email: b.marsh-at-imb.uq.edu.au Name: Brad Marsh
Organization: University of Queensland
Title-Subject: [Filtered] The Asia-Pacific Congress on Electron Tomography
Question: The Asia-Pacific Congress on Electron Tomography (APCET) will be held in Brisbane, Australia, from Saturday January 31 through Wednesday February 4, 2009. This meeting represents the first opportunity to bring together world leaders in the field of electron tomography of molecules and cells outside of Europe or North America, and will foster the exchange of ideas and technical information among biologists, biophysicists, computer scientists, mathematicians, materials scientists and electron optical instrumentation specialists in an open interdisciplinary environment. For more information, please visit:
http://homepage.mac.com/marshbj/APCET/
A reminder that the Early Registration ($500 AUD) Deadline closes 30 September, 2008. Late registration closes (Sunday) 30 November, 2008. Registration is limited to 200 participants.
All conference participants are strongly encouraged to book their hotel accommodation well in advance, as the discounted room rates listed below are strictly subject to availability and only a limited number of rooms can be set aside; the APCET session dates fall within 'high season' for tourism in Brisbane/Australia. Brisbane remains one of Australia's most popular destinations for international tourists, and has been consistently voted Australia's "Most Liveable City". Consequently, hotel rooms in Brisbane are often in short supply, and 100% occupancy is not unusual.
APCET is not able to guarantee accommodation at the secured room rates listed below unless reservations are confirmed by 31 October 2008.
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Email: jeff-at-tss-consulting.com Name: jeff West
Organization: TSS Consulting Ltd.
Title-Subject: [Filtered] Career Opportunity
Question: This confidential search is for a posiiton located in the Northeast. Please reply with interest to the email or phone number below.
We are looking for a candidate with:
ï A Ph.D. in Materials Science or Engineering, Electrical Engineering, Physics, or related discipline ï Significant experience with LED (and, ideally, LD) device design, fabrication and characterization. ï Basic familiarity with packaging approaches for LEDs including device/package interactions
Who is able to
ï Work with a team of material scientists and epitaxial researchers in developing semiconductor lighting and laser solutions based on III-V and III-N materials. ï Take a hand-on, self-directed lead in establishing capability for basic fabrication and characterization of LEDs (and possibly LDs) within the company, based on the companyís proprietary materials technologies ï Work with external device manufacturing partners in demonstrating advanced LED and LDs based on the companyís proprietary materials technologies
Regards: Jeff West Jeff-at-tss-consulting.com 877-489-2425
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Email: renaudgeological-at-execulink.com Name: Jim Renaud
Question: I am looking for a manual diamond scribe/etch to fit on the objective head of my Meiji scope. This scribe replaces one of the objective lenses and allows you to rotate the stage and scribe/etch a circle around a grain or area of interest on a carbon coated polished section. I have seen these used, but have not been able to find one. Does anyone have one they are not using, are willing to part with or sell. Or do you know where I can purchase one. Please let me know.
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Email: jmastrangelo-at-ulbi.com Name: Joe Mastrangelo
Organization: Ultralife
Title-Subject: [Filtered] Replacement for Polaroid sheet film
Question: Greetings all,
Not really a microscopy question, but I know there are many out there on this listserve doing all types of imaging... I thought I would take a shot here.
We have an old Faxitron X-ray cabinet that is still working fine after all these years. We have, to this point, been using Polaroid 54 sheet film to capture images but Polaroid has now discontinued their much of their sheet film line. I scrambled to find a distributor that might have some left but it was to no avail.
Does anyone know of a good alternative available or has my old cabinet finally become completely obsolete?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m78NTphW012656 for {MicroscopyListserverArchive-at-microscopy.com} ; Fri, 8 Aug 2008 18:29:51 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id m78NTpD1012654; Fri, 8 Aug 2008 18:29:51 -0500
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Email: ncfl-at-vt.edu Name: Bill Reynolds
Organization: Nanoscale Characterization and Fabrication Lab / Virginia Tech
Title-Subject: [Filtered] Job Opening
Question: Research Faculty Position for Transmission Electron Microscopist
The Nanoscale Characterization and Fabrication Laboratory within the Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech announces a research faculty opening for a transmission electron microscopist. This person is expected to have completed a Ph.D. degree in an engineering or physical science discipline, and have at least 3 years of TEM research experience. The successful candidate will be in charge of operating an FEI Titan TEM package with associated analytical equipment and software. The new hire will collaborate on research proposals and work with users across the University to plan and execute sophisticated TEM experiments.
We will start to review applications on August 15, 2008, and continue until the position is filled. Applications will be accepted on-line at: http://jobs.vt.edu/applicants/Central?quickFind=189172 Interested candidates should apply to posting number 080758 by submitting a cover letter, CV, and names and addresses of three references.
Virginia Tech is an Equal Opportunity/Equal Access employer.
Dear Group: would anyone know where I can get a copy of the manual for a Precision Scientific Company Model 25 Shaker Bath? This company is a subsidiary of GCA corporation. Thanks so much. Barbara
==============================Original Headers============================== 1, 17 -- From maloneyb-at-fiu.edu Sat Aug 9 09:46:15 2008 1, 17 -- Received: from fmailhost03.isp.att.net (fmailhost03.isp.att.net [204.127.217.103]) 1, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m79EkF2W027889 1, 17 -- for {Microscopy-at-microscopy.com} ; Sat, 9 Aug 2008 09:46:15 -0500 1, 17 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 17 -- by isp.att.net (frfwmhc03) with ESMTP 1, 17 -- id {20080809144614H0300psjfqe} ; Sat, 9 Aug 2008 14:46:14 +0000 1, 17 -- X-Originating-IP: [74.166.189.137] 1, 17 -- Message-ID: {489DAD88.6010208-at-fiu.edu} 1, 17 -- Date: Sat, 09 Aug 2008 10:45:28 -0400 1, 17 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 1, 17 -- MIME-Version: 1.0 1, 17 -- To: Microscopy-at-microscopy.com 1, 17 -- Subject: manual for water shaker bath 1, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
No prep is needed per se. The reference below shows direct FIB/SEM/TEM of bone on a dental implant. We just made sure it was "dry" enough to be put into the vacuum chamber of a DualBeam prior to analysis.
L . Giannuzzi , D . Phifer , N . Giannuzzi , M . Capuano, "Two-Dimensional and 3-Dimensional Analysis of Bone/Dental Implant Interfaces With the Use of Focused Ion Beam and Electron Microscopy," Journal of Oral and Maxillofacial Surgery , Volume 65 , Issue 4 , Pages 737 - 747.
Regards, Lucille Giannuzzi
Disclaimer: I work for FEI Company which makes and sells DualBeams/TEMs used in the reference cited above.
} -----Original Message----- } From: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] } Sent: Wednesday, July 30, 2008 9:32 PM } To: lgiannuzzi-at-comcast.net } Subject: [Microscopy] TEM of mineralized bone } } } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } Hi all, } } Does anyone have a protocol for prep of mineralized bone? We want to } preserve the minerals within the bone so decalcification is not an option. } } Debby } } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.agriculture.purdue.edu/microscopy } } } } } ==============================Original } Headers============================== } 7, 29 -- From dsherman-at-purdue.edu Wed Jul 30 20:24:25 2008 } 7, 29 -- Received: from mailhub128.itcs.purdue.edu } (mailhub128.itcs.purdue.edu [128.210.5.128]) } 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m6V1OP37008626 } 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 20:24:25 - } 0500 } 7, 29 -- Received: from mailhub126.itcs.purdue.edu } (mailhub126.itcs.purdue.edu [128.210.5.126]) } 7, 29 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with } ESMTP id m6V1OOlf012485 } 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 21:24:24 - } 0400 } 7, 29 -- Received: from 1061exfe04a.itap.purdue.edu } (1061exfe04a.itap.purdue.edu [128.210.1.11]) } 7, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange- } outbound) with ESMTP id m6V1OOlt019477 } 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jul 2008 21:24:24 - } 0400 } 7, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by } 1061exfe04a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); } 7, 29 -- Wed, 30 Jul 2008 21:23:49 -0400 } 7, 29 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl } ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu } ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; } 7, 29 -- Thu, 31 Jul 2008 01:23:49 +0000 } 7, 29 -- User-Agent: Microsoft-Entourage/12.11.0.080522 } 7, 29 -- Date: Wed, 30 Jul 2008 21:23:49 -0400 } 7, 29 -- Subject: TEM of mineralized bone } 7, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} } 7, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 7, 29 -- Message-ID: {C4B68C65.1F688%dsherman-at-purdue.edu} } 7, 29 -- Thread-Topic: TEM of mineralized bone } 7, 29 -- Thread-Index: AcjyrBWOQmXEyer/zUm27JgA1FxkJA== } 7, 29 -- Mime-version: 1.0 } 7, 29 -- Content-type: text/plain; } 7, 29 -- charset="US-ASCII" } 7, 29 -- Content-transfer-encoding: 7bit } 7, 29 -- X-OriginalArrivalTime: 31 Jul 2008 01:23:49.0807 (UTC) } FILETIME=[160963F0:01C8F2AC] } 7, 29 -- X-PMX-Version: 5.4.0.320885 } 7, 29 -- X-PerlMx-Virus-Scanned: Yes } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 29 -- From lgiannuzzi-at-comcast.net Sat Aug 9 18:43:46 2008 7, 29 -- Received: from QMTA06.westchester.pa.mail.comcast.net (qmta06.westchester.pa.mail.comcast.net [76.96.62.56]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m79Nhjnl031409 7, 29 -- for {microscopy-at-microscopy.com} ; Sat, 9 Aug 2008 18:43:46 -0500 7, 29 -- Received: from OMTA05.westchester.pa.mail.comcast.net ([76.96.62.43]) 7, 29 -- by QMTA06.westchester.pa.mail.comcast.net with comcast 7, 29 -- id 0N2l1a0060vyq2s56PjlJs; Sat, 09 Aug 2008 23:43:45 +0000 7, 29 -- Received: from Lu ([69.247.48.161]) 7, 29 -- by OMTA05.westchester.pa.mail.comcast.net with comcast 7, 29 -- id 0Pjl1a0053Ug5ml3RPjlyk; Sat, 09 Aug 2008 23:43:45 +0000 7, 29 -- X-Authority-Analysis: v=1.0 c=1 a=qjFgFpALMCcA:10 a=huiPnjBNAOcA:10 7, 29 -- a=Zx37jsudAAAA:8 a=35Thz0BbAAAA:8 a=PCRDLm_fJVw2oNKqxB0A:9 7, 29 -- a=y8K1R6iZFVtOOa6ftEIA:7 a=8HlcR8laWvBTt0HYdjJaUUekb48A:4 a=AtnM69iVAwYA:10 7, 29 -- a=-DdyyhJ99MMA:10 a=si9q_4b84H0A:10 a=G91thX30KR8A:10 a=50e4U0PicR4A:10 7, 29 -- From: "Lucille A. Giannuzzi" {lgiannuzzi-at-comcast.net} 7, 29 -- To: {dsherman-at-purdue.edu} 7, 29 -- Cc: {Microscopy-at-microscopy.com} 7, 29 -- References: {200807310131.m6V1VUL6018121-at-ns.microscopy.com} 7, 29 -- Subject: RE: [Microscopy] TEM of mineralized bone 7, 29 -- Date: Sat, 9 Aug 2008 19:41:32 -0400 7, 29 -- Message-ID: {006701c8fa79$748ddc60$6401a8c0-at-Lu} 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="us-ascii" 7, 29 -- Content-Transfer-Encoding: 7bit 7, 29 -- X-Mailer: Microsoft Office Outlook 11 7, 29 -- x-mimeole: Produced By Microsoft MimeOLE V6.00.2900.3198 7, 29 -- Thread-Index: AcjyrSjtN1wTxxv4RNOlozzBzkZ5DQHyldwg 7, 29 -- In-Reply-To: {200807310131.m6V1VUL6018121-at-ns.microscopy.com} ==============================End of - Headers==============================
The 2008 Fall Meeting of the American Geophysical Union (San Francisco, Dec. 15-19; http://www.agu.org/meetings/fm08/) will include a session entitled "Atmospheric Aerosols and Electron Microscopy". As co-conveners of the session, Joe Conny and I hope that you will consider submitting an abstract for the meeting. I think this may be the first time the AGU has had a session on our topic. Hopefully it will be well received and attended and will be the first of many such sessions at AGU. Session A20 is advertised as follows:
Atmospheric Aerosols and Electron Microscopy
This session invites studies of an understanding of atmospheric aerosols through the use of electron microscopy and x-ray microanalysis. Studies include but are not limited to microscopic determinations of particle size, morphology, and chemical composition to reveal sources of anthropogenic aerosols vs. natural aerosols and to reveal links between climatic effects of aerosols and the physical and chemical properties of individual particles.
Key Words 0305 Aerosols and Particles 0345 Pollution: Urban and Regional 0394 Instruments and Techniques 1029 Composition of Aerosols and Dust Particles (Geochemistry) 0365 Troposphere: Composition and Chemistry
We don't know yet if the session will be oral or poster, but the more abstracts that are submitted, the more likely that it will be an oral session. Time is short: Abstracts are due September 10 and acceptances sent on Oct. 23-24. Please go to http://submissions3.agu.org/submission/subm-ins.htm for instructions on submitting an abstract. If you are interested in submitting an abstract, please let me know by email so that I can gauge the level of interest. And please forward this email to any interested colleagues.
Finally, Joe and I must identify a few outstanding people to be "Invited Speakers" by August 18. Your suggestions would be most welcome, including nominating yourselves.
Thanks very much for your interest and help in getting the word around - I hope we'll see you in San Francisco!
Robert Willis, Ph.D. US Environmental Protection Agency National Exposure Research Laboratory Environmental Characterization and Apportionment Branch MD E205-03 Research Triangle Park, NC 27711 Tel: 919-541-2809 Fax: 919-541-0960 willis.robert-at-epa.gov
Joseph M. Conny, Ph.D. Surface and Microanalysis Science Division National Institute of Standards and Technology 100 Bureau Drive Stop 8372 Gaithersburg, MD 20899-8372 301-975-3932 fax 301-926-6689 email joseph.conny-at-nist.gov
==============================Original Headers============================== 12, 27 -- From Willis.Robert-at-epamail.epa.gov Mon Aug 11 08:10:14 2008 12, 27 -- Received: from mblast01.rtp.epa.gov (mblast01.rtp.epa.gov [134.67.221.151]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BDAEgL004505 12, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 08:10:14 -0500 12, 27 -- Received: from mblast01.rtp.epa.gov (localhost.localdomain [127.0.0.1]) 12, 27 -- by localhost (Postfix) with SMTP id CB0C44441A 12, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 09:10:13 -0400 (EDT) 12, 27 -- Received: from mintra01.rtp.epa.gov (mintra01.rtp.epa.gov [134.67.221.153]) 12, 27 -- by mblast01.rtp.epa.gov (Postfix) with ESMTP id C644344348 12, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 09:10:13 -0400 (EDT) 12, 27 -- Received: from mintra01.rtp.epa.gov (localhost.localdomain [127.0.0.1]) 12, 27 -- by localhost (Postfix) with SMTP id BA84D4444A 12, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 09:10:13 -0400 (EDT) 12, 27 -- Received: from epahub12.rtp.epa.gov (epahub12.rtp.epa.gov [134.67.213.53]) 12, 27 -- by mintra01.rtp.epa.gov (Postfix) with ESMTP id B2599443BE 12, 27 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 09:10:13 -0400 (EDT) 12, 27 -- Subject: Atmospheric Aerosols and Electron Microscopy Session at Fall AGU Meeting in 12, 27 -- San Francisco 12, 27 -- To: Microscopy-at-Microscopy.Com 12, 27 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 12, 27 -- Message-ID: {OF4F97545E.D31844AE-ON852574A2.0047E900-852574A2.00485842-at-epamail.epa.gov} 12, 27 -- From: Willis.Robert-at-epamail.epa.gov 12, 27 -- Date: Mon, 11 Aug 2008 09:10:11 -0400 12, 27 -- X-MIMETrack: Serialize by Router on EPAHUB12/USEPA/US(Release 7.0.3|September 26, 2007) at 12, 27 -- 08/11/2008 09:10:13 AM 12, 27 -- MIME-Version: 1.0 12, 27 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Given an inverted microscope (metallograph) and a properly prepared (flat) metallographic specimen, is it unreasonable to expect the entire field of view to be in focus?
I recently replaced my ancient Olympus PME metallograph with a modern metallograph from a highly regarded manufacturer. At low mag, through 200x, all is well. At 500x and 1,000x I can get the middle, or top, or bottom of the field of view in focus; maybe two at a time, but not all three. This shows up on the digital images, not just through the eyepieces. I can get good pictures by using the software to take slices and stitch them together, but I think this ought not to be necessary.
The Vendor's representatives agree that the microscope is not right, but they (two sales people and two Field Service Engineers) have not been able to figure out what the problem is. They are "working on it" (thinking about it) but it seemed prudent to me to ask y'all, since there are many very knowledgeable people on the Listserv (I'm embarrassed to always write for help, never having any to offer - but know my limitations).
My own simple-minded view is that the stage may not be perpendicular to the light path. We have tried adjusting (i.e. "fooled with") the stage setscrews to no avail.
Suspecting that the specimens were not really flat (although I've been doing this for 30 years), we tried a mirror that has some small scratches - not featureless. Problem is not specimen flatness, out-of-focus persists. Also, the specimens look right on the old Olympus. If I still could get Polaroid film, I'd stick with the old microscope and just keep scanning the pictures.
So - any ideas of suggestions are very welcome. Thanks in advance for your help and/or guidance.
Regards, Andrew Werner {werner-at-rosharon.oilfield.slb.com}
==============================Original Headers============================== 8, 24 -- From werner-at-rosharon.oilfield.slb.com Mon Aug 11 09:12:39 2008 8, 24 -- Received: from us1061mta01.mail.slb.com (usxsl051.slb.atosorigin-asp.com [199.6.139.31]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BECcPV019948 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:38 -0500 8, 24 -- Received: from us1061mta01.mail.slb.com (localhost.localdomain [127.0.0.1]) 8, 24 -- by localhost (Postfix) with SMTP id D4A3257840D 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:20 -0500 (CDT) 8, 24 -- Received: from usxsl052.slb.atosorigin-asp.com (usxsl052dmz.slb.atosorigin-asp.com [199.6.139.167]) 8, 24 -- by us1061mta01.mail.slb.com (Postfix) with ESMTP id C282A578486 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:17 -0500 (CDT) 8, 24 -- Received: from WERNER1-OFS1.rosharon.oilfield.slb.com ([163.188.47.162]) 8, 24 -- by us085mbx01.slb.atosorigin-asp.com 8, 24 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 8, 24 -- with ESMTPSA id {0K5F0019YWSHTW50-at-us085mbx01.slb.atosorigin-asp.com} for 8, 24 -- Microscopy-at-microscopy.com; Mon, 11 Aug 2008 14:12:17 +0000 (GMT) 8, 24 -- Date: Mon, 11 Aug 2008 09:12:16 -0500 8, 24 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} 8, 24 -- Subject: LM - field of view / focus question 8, 24 -- To: Microscopy-at-microscopy.com 8, 24 -- Message-id: {0K5F001A0WSHTW50-at-us085mbx01.slb.atosorigin-asp.com} 8, 24 -- MIME-version: 1.0 8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 8, 24 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
I have worked on many "old" PME's as well as a host of other metallographs.
Yes, you should expect to have a flat field of view at all magnifications.
An option I have used quite successfully (in my opinion) is the take a digital camera and get an adaptor that goes into the eyepiece. then you can just take out one of the eyepieces of the PME and shoot digital photos directly. If you want more info, or photos of my particular set-up, drop me an email off-list and I'll send you some photos of how I got around this. I tried using some of the photo ports on the PME to hook the digital camera through them but there are some quite difficult hoops on that route. I finally settled for the eyepiece adaptor and that seems to work quite well.
dj
On Mon, 11 Aug 2008, werner-at-rosharon.oilfield.slb.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Given an inverted microscope (metallograph) and a properly prepared } (flat) metallographic specimen, is it unreasonable to expect the } entire field of view to be in focus? } } I recently replaced my ancient Olympus PME metallograph with a modern } metallograph from a highly regarded manufacturer. At low mag, } through 200x, all is well. At 500x and 1,000x I can get the middle, } or top, or bottom of the field of view in focus; maybe two at a time, } but not all three. This shows up on the digital images, not just } through the eyepieces. I can get good pictures by using the software } to take slices and stitch them together, but I think this ought not } to be necessary. } } The Vendor's representatives agree that the microscope is not right, } but they (two sales people and two Field Service Engineers) have not } been able to figure out what the problem is. They are "working on } it" (thinking about it) but it seemed prudent to me to ask y'all, } since there are many very knowledgeable people on the Listserv (I'm } embarrassed to always write for help, never having any to offer - but } know my limitations). } } My own simple-minded view is that the stage may not be perpendicular } to the light path. We have tried adjusting (i.e. "fooled with") the } stage setscrews to no avail. } } Suspecting that the specimens were not really flat (although I've } been doing this for 30 years), we tried a mirror that has some small } scratches - not featureless. Problem is not specimen flatness, } out-of-focus persists. Also, the specimens look right on the old } Olympus. If I still could get Polaroid film, I'd stick with the old } microscope and just keep scanning the pictures. } } So - any ideas of suggestions are very welcome. Thanks in advance } for your help and/or guidance. } } Regards, } Andrew Werner } {werner-at-rosharon.oilfield.slb.com} } } } ==============================Original Headers============================== } 8, 24 -- From werner-at-rosharon.oilfield.slb.com Mon Aug 11 09:12:39 2008 } 8, 24 -- Received: from us1061mta01.mail.slb.com (usxsl051.slb.atosorigin-asp.com [199.6.139.31]) } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BECcPV019948 } 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:38 -0500 } 8, 24 -- Received: from us1061mta01.mail.slb.com (localhost.localdomain [127.0.0.1]) } 8, 24 -- by localhost (Postfix) with SMTP id D4A3257840D } 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:20 -0500 (CDT) } 8, 24 -- Received: from usxsl052.slb.atosorigin-asp.com (usxsl052dmz.slb.atosorigin-asp.com [199.6.139.167]) } 8, 24 -- by us1061mta01.mail.slb.com (Postfix) with ESMTP id C282A578486 } 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:17 -0500 (CDT) } 8, 24 -- Received: from WERNER1-OFS1.rosharon.oilfield.slb.com ([163.188.47.162]) } 8, 24 -- by us085mbx01.slb.atosorigin-asp.com } 8, 24 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) } 8, 24 -- with ESMTPSA id {0K5F0019YWSHTW50-at-us085mbx01.slb.atosorigin-asp.com} for } 8, 24 -- Microscopy-at-microscopy.com; Mon, 11 Aug 2008 14:12:17 +0000 (GMT) } 8, 24 -- Date: Mon, 11 Aug 2008 09:12:16 -0500 } 8, 24 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} } 8, 24 -- Subject: LM - field of view / focus question } 8, 24 -- To: Microscopy-at-microscopy.com } 8, 24 -- Message-id: {0K5F001A0WSHTW50-at-us085mbx01.slb.atosorigin-asp.com} } 8, 24 -- MIME-version: 1.0 } 8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed } 8, 24 -- Content-transfer-encoding: 7BIT } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 19 -- From dljones-at-bestweb.net Mon Aug 11 09:32:36 2008 7, 19 -- Received: from smtp3.bestweb.net (smtp3.bestweb.net [209.94.103.43]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BEWZ2F001429 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:32:36 -0500 7, 19 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202]) 7, 19 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 19 -- (No client certificate requested) 7, 19 -- by smtp3.bestweb.net (Postfix) with ESMTPS id 395A55C23; 7, 19 -- Mon, 11 Aug 2008 10:32:34 -0400 (EDT) 7, 19 -- Date: Mon, 11 Aug 2008 14:17:30 +0000 (UTC) 7, 19 -- From: dljones {dljones-at-bestweb.net} 7, 19 -- To: werner-at-rosharon.oilfield.slb.com 7, 19 -- cc: microscopy-at-microscopy.com 7, 19 -- Subject: Re: [Microscopy] LM - field of view / focus question 7, 19 -- In-Reply-To: {200808111417.m7BEHMAh025647-at-ns.microscopy.com} 7, 19 -- Message-ID: {Pine.BSF.4.64.0808111411480.58031-at-monet.bestweb.net} 7, 19 -- References: {200808111417.m7BEHMAh025647-at-ns.microscopy.com} 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Andrew, Not a light expert, here, but it would seem to me that if it's only certain objectives, then maybe that might be where to look. On the other hand, can you get a camera adapter for a digital camera on the old metallograph?
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: werner-at-rosharon.oilfield.slb.com [mailto:werner-at-rosharon.oilfield.slb.com] Sent: Monday, August 11, 2008 10:16 AM To: kenconverse-at-qualityimages.biz
Given an inverted microscope (metallograph) and a properly prepared (flat) metallographic specimen, is it unreasonable to expect the entire field of view to be in focus?
I recently replaced my ancient Olympus PME metallograph with a modern metallograph from a highly regarded manufacturer. At low mag, through 200x, all is well. At 500x and 1,000x I can get the middle, or top, or bottom of the field of view in focus; maybe two at a time, but not all three. This shows up on the digital images, not just through the eyepieces. I can get good pictures by using the software to take slices and stitch them together, but I think this ought not to be necessary.
The Vendor's representatives agree that the microscope is not right, but they (two sales people and two Field Service Engineers) have not been able to figure out what the problem is. They are "working on it" (thinking about it) but it seemed prudent to me to ask y'all, since there are many very knowledgeable people on the Listserv (I'm embarrassed to always write for help, never having any to offer - but know my limitations).
My own simple-minded view is that the stage may not be perpendicular to the light path. We have tried adjusting (i.e. "fooled with") the stage setscrews to no avail.
Suspecting that the specimens were not really flat (although I've been doing this for 30 years), we tried a mirror that has some small scratches - not featureless. Problem is not specimen flatness, out-of-focus persists. Also, the specimens look right on the old Olympus. If I still could get Polaroid film, I'd stick with the old microscope and just keep scanning the pictures.
So - any ideas of suggestions are very welcome. Thanks in advance for your help and/or guidance.
Regards, Andrew Werner {werner-at-rosharon.oilfield.slb.com}
==============================Original Headers============================== 8, 24 -- From werner-at-rosharon.oilfield.slb.com Mon Aug 11 09:12:39 2008 8, 24 -- Received: from us1061mta01.mail.slb.com (usxsl051.slb.atosorigin-asp.com [199.6.139.31]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BECcPV019948 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:38 -0500 8, 24 -- Received: from us1061mta01.mail.slb.com (localhost.localdomain [127.0.0.1]) 8, 24 -- by localhost (Postfix) with SMTP id D4A3257840D 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:20 -0500 (CDT) 8, 24 -- Received: from usxsl052.slb.atosorigin-asp.com (usxsl052dmz.slb.atosorigin-asp.com [199.6.139.167]) 8, 24 -- by us1061mta01.mail.slb.com (Postfix) with ESMTP id C282A578486 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:17 -0500 (CDT) 8, 24 -- Received: from WERNER1-OFS1.rosharon.oilfield.slb.com ([163.188.47.162]) 8, 24 -- by us085mbx01.slb.atosorigin-asp.com 8, 24 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 8, 24 -- with ESMTPSA id {0K5F0019YWSHTW50-at-us085mbx01.slb.atosorigin-asp.com} for 8, 24 -- Microscopy-at-microscopy.com; Mon, 11 Aug 2008 14:12:17 +0000 (GMT) 8, 24 -- Date: Mon, 11 Aug 2008 09:12:16 -0500 8, 24 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} 8, 24 -- Subject: LM - field of view / focus question 8, 24 -- To: Microscopy-at-microscopy.com 8, 24 -- Message-id: {0K5F001A0WSHTW50-at-us085mbx01.slb.atosorigin-asp.com} 8, 24 -- MIME-version: 1.0 8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed 8, 24 -- Content-transfer-encoding: 7BIT ==============================End of - Headers==============================
==============================Original Headers============================== 20, 26 -- From kenconverse-at-qualityimages.biz Mon Aug 11 09:40:51 2008 20, 26 -- Received: from mta13.adelphia.net (mta13.adelphia.net [68.168.78.44]) 20, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BEeoBt014644 20, 26 -- for {microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:40:50 -0500 20, 26 -- Received: from Ken ([72.227.100.25]) by mta13.adelphia.net 20, 26 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 20, 26 -- id {20080811143815.INH7723.mta13.adelphia.net-at-Ken} ; 20, 26 -- Mon, 11 Aug 2008 10:38:15 -0400 20, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 26 -- To: {werner-at-rosharon.oilfield.slb.com} , 20, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 20, 26 -- Subject: RE: [Microscopy] LM - field of view / focus question 20, 26 -- Date: Mon, 11 Aug 2008 10:40:42 -0400 20, 26 -- Message-ID: {66E43EE7BF76418C9C37319DC1DADCD3-at-Ken} 20, 26 -- MIME-Version: 1.0 20, 26 -- Content-Type: text/plain; 20, 26 -- charset="us-ascii" 20, 26 -- X-Priority: 3 (Normal) 20, 26 -- X-MSMail-Priority: Normal 20, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 20, 26 -- Importance: Normal 20, 26 -- Thread-Index: Acj7vM4VlTop7GuJTIK+JNVbqpQv9gAAtb0g 20, 26 -- In-Reply-To: {200808111416.m7BEGBpP024273-at-ns.microscopy.com} 20, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 20, 26 -- Content-Transfer-Encoding: 8bit 20, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7BEeoBt014644 ==============================End of - Headers==============================
I agree that something sounds out of whack. Have you tried "shimming" one side of your sample to see if that improves the focus situation? If the stage is out of alignment, that should tell you in what direction and by how much.
Personally, I think the "highly regarded manufacturer" ought to replace the scope with a brand new one, and be done with it.
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635 robert.zonis-at-sanford.com www.papermate.com
-----Original Message----- X-from: werner-at-rosharon.oilfield.slb.com [mailto:werner-at-rosharon.oilfield.slb.com] Sent: Monday, August 11, 2008 9:21 AM To: Zonis, Robert
The problem may be the alignment of the stage to the nosepiece as a whole, but it might also be due to the alignment of the objectives within the nosepiece. Take a series of photos at different focal depths to determine the direction and magnitude of the focal shift. Then switch the offending objective to a different position in the nosepiece and repeat the procedure. If you get different results, the objective mounts may not be correctly aligned. If you are lucky, you might be able to fix the problem with a new nosepiece. You might also ask your microscope rep to bring another objective and see if it gives the same results as the one you have.
Until you get the problem fixed you might want to use software like CombineZ or Helicon Focus, which combine Z stacks automatically. This might be easier to use than montage software, if that is what you are using now.
Ralph Common Michigan State University
==============================Original Headers============================== 5, 24 -- From rcommon-at-msu.edu Mon Aug 11 10:20:40 2008 5, 24 -- Received: from sys23.mail.msu.edu (sys23.mail.msu.edu [35.9.75.123]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BFKemZ009693 5, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 10:20:40 -0500 5, 24 -- Received: from [35.9.122.125] (helo=emlab) 5, 24 -- by sys23.mail.msu.edu with esmtpsa (Exim 4.63 #1) 5, 24 -- (TLSv1:RC4-MD5:128) 5, 24 -- id 1KSZCK-0007wh-2h 5, 24 -- for Microscopy-at-microscopy.com; Mon, 11 Aug 2008 11:20:40 -0400 5, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 5, 24 -- To: {Microscopy-at-microscopy.com} 5, 24 -- Subject: LM - field of view / focus question 5, 24 -- Date: Mon, 11 Aug 2008 11:20:49 -0400 5, 24 -- Message-ID: {00a001c8fbc5$d6a1dbc0$7d7a0923-at-msu.edu} 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="iso-8859-1" 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Priority: 3 (Normal) 5, 24 -- X-MSMail-Priority: Normal 5, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 5, 24 -- Importance: Normal 5, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1914 5, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Having read through some of the suggestions, my take would be that you spent a fair amount of money on the scope and you probably spent a lot of time troubleshooting this to the level that you already have.
Why spin your wheels even more to pinpoint the offending component(s)? I would just demand that the dealer replace the whole scope. It sounds like you have been inconvenienced enough.
If you don't get satisfaction through your local dealer, then I would call the NA headquarters. If they are reputable, then they will want to satisfy you and investigate the problem on their own.
Alan Stone ASTON Metallurgical Services
At 09:14 AM 8/11/2008, you wrote:
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==============================Original Headers============================== 11, 22 -- From as-at-astonmet.com Mon Aug 11 10:29:09 2008 11, 22 -- Received: from outbound2.mail.tds.net (outbound2.mail.tds.net [216.170.230.92]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BFT96s022840 11, 22 -- for {microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 10:29:09 -0500 11, 22 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32]) 11, 22 -- by outbound2.mail.tds.net (8.13.6/8.13.4) with ESMTP id m7BFT6gf028735; 11, 22 -- Mon, 11 Aug 2008 10:29:07 -0500 11, 22 -- Received: from mozart.astonmet.com ([69.11.219.4]) 11, 22 -- by outaamta02.mail.tds.net with ESMTP 11, 22 -- id {20080811152901.WFEK4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} ; 11, 22 -- Mon, 11 Aug 2008 10:29:01 -0500 11, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 22 -- Date: Mon, 11 Aug 2008 10:28:27 -0500 11, 22 -- To: werner-at-rosharon.oilfield.slb.com 11, 22 -- From: Alan Stone {as-at-astonmet.com} 11, 22 -- Subject: Re: [Microscopy] LM - field of view / focus question 11, 22 -- Cc: microscopy-at-microscopy.com 11, 22 -- In-Reply-To: {200808111414.m7BEEF8k021763-at-ns.microscopy.com} 11, 22 -- References: {200808111414.m7BEEF8k021763-at-ns.microscopy.com} 11, 22 -- Mime-Version: 1.0 11, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 11, 22 -- Message-Id: {20080811152901.WFEK4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} ==============================End of - Headers==============================
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Hi Andrew,
I agree with the comments regarding putting it back on the vendor. A modern microscope should perform as well as your older microscope. As you have determined, something is out of alignment. I learned a long time ago, not to allow vendors too much leeway, as many will take advantage of it. If they cannot correct the problem with an adjustment within a reasonable time, then they should provide a properly functioning system, for which you have already paid.
Good Luck, Darrell
werner-at-rosharon.oilfield.slb.com wrote on 08/11/2008 10:13:46 AM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Given an inverted microscope (metallograph) and a properly prepared } (flat) metallographic specimen, is it unreasonable to expect the } entire field of view to be in focus? } } I recently replaced my ancient Olympus PME metallograph with a modern } metallograph from a highly regarded manufacturer. At low mag, } through 200x, all is well. At 500x and 1,000x I can get the middle, } or top, or bottom of the field of view in focus; maybe two at a time, } but not all three. This shows up on the digital images, not just } through the eyepieces. I can get good pictures by using the software } to take slices and stitch them together, but I think this ought not } to be necessary. } } The Vendor's representatives agree that the microscope is not right, } but they (two sales people and two Field Service Engineers) have not } been able to figure out what the problem is. They are "working on } it" (thinking about it) but it seemed prudent to me to ask y'all, } since there are many very knowledgeable people on the Listserv (I'm } embarrassed to always write for help, never having any to offer - but } know my limitations). } } My own simple-minded view is that the stage may not be perpendicular } to the light path. We have tried adjusting (i.e. "fooled with") the } stage setscrews to no avail. } } Suspecting that the specimens were not really flat (although I've } been doing this for 30 years), we tried a mirror that has some small } scratches - not featureless. Problem is not specimen flatness, } out-of-focus persists. Also, the specimens look right on the old } Olympus. If I still could get Polaroid film, I'd stick with the old } microscope and just keep scanning the pictures. } } So - any ideas of suggestions are very welcome. Thanks in advance } for your help and/or guidance. } } Regards, } Andrew Werner } {werner-at-rosharon.oilfield.slb.com} } } } ==============================Original Headers============================== } 8, 24 -- From werner-at-rosharon.oilfield.slb.com Mon Aug 11 09:12:39 2008 } 8, 24 -- Received: from us1061mta01.mail.slb.com (usxsl051.slb. } atosorigin-asp.com [199.6.139.31]) } 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m7BECcPV019948 } 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12:38 -0500 } 8, 24 -- Received: from us1061mta01.mail.slb.com (localhost. } localdomain [127.0.0.1]) } 8, 24 -- by localhost (Postfix) with SMTP id D4A3257840D } 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12: } 20 -0500 (CDT) } 8, 24 -- Received: from usxsl052.slb.atosorigin-asp.com } (usxsl052dmz.slb.atosorigin-asp.com [199.6.139.167]) } 8, 24 -- by us1061mta01.mail.slb.com (Postfix) with ESMTP id C282A578486 } 8, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Aug 2008 09:12: } 17 -0500 (CDT) } 8, 24 -- Received: from WERNER1-OFS1.rosharon.oilfield.slb.com } ([163.188.47.162]) } 8, 24 -- by us085mbx01.slb.atosorigin-asp.com } 8, 24 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) } 8, 24 -- with ESMTPSA id {0K5F0019YWSHTW50-at-us085mbx01.slb. } atosorigin-asp.com} for } 8, 24 -- Microscopy-at-microscopy.com; Mon, 11 Aug 2008 14:12:17 +0000 (GMT) } 8, 24 -- Date: Mon, 11 Aug 2008 09:12:16 -0500 } 8, 24 -- From: Andrew Werner {werner-at-rosharon.oilfield.slb.com} } 8, 24 -- Subject: LM - field of view / focus question } 8, 24 -- To: Microscopy-at-microscopy.com } 8, 24 -- Message-id: {0K5F001A0WSHTW50-at-us085mbx01.slb.atosorigin-asp.com} } 8, 24 -- MIME-version: 1.0 } 8, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 8, 24 -- Content-type: text/plain; charset=us-ascii; format=flowed } 8, 24 -- Content-transfer-encoding: 7BIT } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 28 -- From milesd-at-us.ibm.com Mon Aug 11 12:39:17 2008 6, 28 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7BHdH5m021551 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 12:39:17 -0500 6, 28 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 6, 28 -- by e2.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id m7BHdF9p013919 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 13:39:15 -0400 6, 28 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 6, 28 -- by d01relay04.pok.ibm.com (8.13.8/8.13.8/NCO v9.0) with ESMTP id m7BHdFJC210024 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 13:39:15 -0400 6, 28 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 6, 28 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id m7BHdFR7016698 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 13:39:15 -0400 6, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 6, 28 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id m7BHdFLZ016695 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 11 Aug 2008 13:39:15 -0400 6, 28 -- In-Reply-To: {200808111413.m7BEDk1G021051-at-ns.microscopy.com} 6, 28 -- To: Microscopy-at-Microscopy.Com 6, 28 -- MIME-Version: 1.0 6, 28 -- Subject: Re: [Microscopy] LM - field of view / focus question 6, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 6, 28 -- Message-ID: {OF062587E1.6826CDB9-ON852574A2.005F5467-852574A2.0060F676-at-us.ibm.com} 6, 28 -- From: Darrell Miles {milesd-at-us.ibm.com} 6, 28 -- Date: Mon, 11 Aug 2008 13:39:15 -0400 6, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1|February 07, 2008) at 6, 28 -- 08/11/2008 13:39:16, 6, 28 -- Serialize complete at 08/11/2008 13:39:16 6, 28 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
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There is a long list of commerical vendors on the following WWW site
http://www.microscopy.com
At the bottom left select the navigation link to WWW sites then use the search engine options to customize your search for commerical vendors who do TEM and service/contract work. You will find links to their WWW sites with further contact details.
Nestor Your Friendly Neighborhood SysOp
At 7:44 PM -0500 8/11/08, valerie_cullen-at-hotmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 12, 13 -- From zaluzec-at-microscopy.com Tue Aug 12 08:53:05 2008 12, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7CDr4Ma024449; 12, 13 -- Tue, 12 Aug 2008 08:53:05 -0500 12, 13 -- Mime-Version: 1.0 12, 13 -- Message-Id: {p06240807c4c744b86672-at-[206.69.208.22]} 12, 13 -- In-Reply-To: {200808120044.m7C0ioRW031383-at-ns.microscopy.com} 12, 13 -- References: {200808120044.m7C0ioRW031383-at-ns.microscopy.com} 12, 13 -- Date: Tue, 12 Aug 2008 08:53:04 -0500 12, 13 -- To: valerie_cullen-at-hotmail.com, microscopy-at-microscopy.com 12, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 12, 13 -- Subject: EM vendors 12, 13 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Registration is now open for the 3d International Workshop on Piezoresponse Force Microscopy, to be held at Center for Nanophase Materials Sciences at Oak Ridge National Laboratory, TN, on September 22-24. The registration information is available at www.cnms.ornl.gov.
The materials of the previous two workshops at ORNL and EPFL (lectures, photoes, and agenda) are available on the CNMS Functional Imaging group website at imaging.ornl.gov, in the section "Knowledge Base"
Several postdoctoral positions in dynamic SPM, PFM, and oxide growth are announced in the "Positions" section. Please contact Sergei Kalinin (sergei2-at-ornl.gov) for details regarding positions and the workshop.
Yours Sergei V. Kalinin
==============================Original Headers============================== 5, 24 -- From sergei2-at-ornl.gov Tue Aug 12 15:32:45 2008 5, 24 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7CKWj3V021155 5, 24 -- for {microscopy-at-microscopy.com} ; Tue, 12 Aug 2008 15:32:45 -0500 5, 24 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 5, 24 -- by emroute3.ornl.gov (PMDF V6.3-x14 #31501) 5, 24 -- with ESMTP id {0K5I0051D92K1X-at-emroute3.ornl.gov} for 5, 24 -- microscopy-at-microscopy.com; Tue, 12 Aug 2008 16:32:44 -0400 (EDT) 5, 24 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 5, 24 -- (PMDF V6.3-x14 #31501) id {0K5I0050192K6D-at-emroute3.ornl.gov} for 5, 24 -- microscopy-at-microscopy.com; Tue, 12 Aug 2008 16:32:44 -0400 (EDT) 5, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 5, 24 -- by emroute3.ornl.gov (PMDF V6.3-x14 #31501) 5, 24 -- with ESMTP id {0K5I0047692KI8-at-emroute3.ornl.gov} for 5, 24 -- microscopy-at-microscopy.com; Tue, 12 Aug 2008 16:32:44 -0400 (EDT) 5, 24 -- Date: Tue, 12 Aug 2008 16:32:44 -0400 5, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 5, 24 -- Subject: PFM Workshop/positions open 5, 24 -- To: microscopy-at-microscopy.com 5, 24 -- Message-id: {48A1F36C.4020700-at-ornl.gov} 5, 24 -- MIME-version: 1.0 5, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 5, 24 -- Content-transfer-encoding: 7bit 5, 24 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ecoagripolicy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ecoagripolicy-at-gmail.com Name: Rachel
Title-Subject: [Filtered] Suberin- lignin
Question: Hi all, I want to localise and/or differentiate the arrangement of lignin and suberin in plant root cell walls. I have used bright field and fluorescence microscopy to confirm the presence of lignin and suberin. But am not sure which technique will be more appropriate to give me more information on the positioning/arrangment of suberin and lignin? Regards, Rachel.
The paper by Biggs (1985, Stain Tech. 60: 299-304) differentiates suberin from lignin by assaying for quenching of lignin autofluorescence in suberin via staining with Sudan dyes. This worked for us in study of Casuarina root nodule infected cells, so I can vouch for its efficacy.
Cheers,
Howard
R. Howard Berg, Ph.D. Director, Integrated Microscopy Facility Danforth Plant Science Center 975 N. Warson Rd. St. Louis, MO 63132
ph 314-587-1261 fx 314-587-1361 cell 314-378-2409 rhberg-at-danforthcenter.org www.danforthcenter.org visit this educational resource: http://www.danforthcenter.org/Cells/
On Aug 13, 2008, at 9:21 AM, ecoagripolicy-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both ecoagripolicy-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ecoagripolicy-at-gmail.com } Name: Rachel } } Title-Subject: [Filtered] Suberin- lignin } } Question: Hi all, } I want to localise and/or differentiate the arrangement of lignin and } suberin in plant root cell walls. I have used bright field and } fluorescence microscopy to confirm the presence of lignin and } suberin. But am not sure which technique will be more appropriate to } give me more information on the positioning/arrangment of suberin and } lignin? } Regards, } Rachel. } } Login Host: 130.95.128.51 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 5, 11 -- From zaluzec-at-microscopy.com Wed Aug 13 09:19:26 2008 } 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m7DEJPNq013092 } 5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:19:26 } -0500 } 5, 11 -- Mime-Version: 1.0 } 5, 11 -- Message-Id: {p06240800c4c89dd94be3-at-[206.69.208.22]} } 5, 11 -- Date: Wed, 13 Aug 2008 09:19:24 -0500 } 5, 11 -- To: microscopy-at-microscopy.com } 5, 11 -- From: ecoagripolicy-at-gmail.com (by way of } MicroscopyListserver) } 5, 11 -- Subject: viaWWW: Suberin- lignin } 5, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 15, 24 -- From RHBerg-at-danforthcenter.org Wed Aug 13 09:59:23 2008 15, 24 -- Received: from spm1.ddpsc.org (spm1.ddpsc.org [65.254.111.26]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7DExMOG027462 15, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:59:23 -0500 15, 24 -- Received: from spm1.ddpsc.org (127.0.0.1) by spm1.ddpsc.org (MlfMTA v3.2r9) id hkbrcm0171sk for {Microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:59:22 -0500 (envelope-from {RHBerg-at-danforthcenter.org} ) 15, 24 -- Received: from mail1.ddpsc.org ([10.101.0.40]) 15, 24 -- by spm1.ddpsc.org (SonicWALL 6.1.0.9597) 15, 24 -- with ESMTP; Wed, 13 Aug 2008 09:59:22 -0500 15, 24 -- Received: from [10.14.0.126] ([10.14.0.126]) by mail1.ddpsc.org with Microsoft SMTPSVC(6.0.3790.3959); 15, 24 -- Wed, 13 Aug 2008 09:59:22 -0500 15, 24 -- Message-Id: {01060D5E-F697-462C-9561-8EDFAF4E8E7C-at-danforthcenter.org} 15, 24 -- From: Howard Berg {rhberg-at-danforthcenter.org} 15, 24 -- To: ecoagripolicy-at-gmail.com, Microscopy-at-microscopy.com 15, 24 -- In-Reply-To: {200808131421.m7DELbTA015439-at-ns.microscopy.com} 15, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 15, 24 -- Content-Transfer-Encoding: 7bit 15, 24 -- Mime-Version: 1.0 (Apple Message framework v926) 15, 24 -- Subject: Re: [Microscopy] viaWWW: Suberin- lignin 15, 24 -- Date: Wed, 13 Aug 2008 09:59:22 -0500 15, 24 -- References: {200808131421.m7DELbTA015439-at-ns.microscopy.com} 15, 24 -- X-Mailer: Apple Mail (2.926) 15, 24 -- X-OriginalArrivalTime: 13 Aug 2008 14:59:22.0354 (UTC) FILETIME=[2B7A4520:01C8FD55] 15, 24 -- X-Mlf-Version: 6.1.0.9597 15, 24 -- X-Mlf-UniqueId: o200808131459220062092 ==============================End of - Headers==============================
You can also use berberine sulfate to pick up only suberin - see papers by Mark Brundrett. In cereal roots, crystal violet is a good counterstain that quenches fluorescence from other walls after berberine staining.
cheers, Rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
On 14/8/08 1:04 AM, "rhberg-at-danforthcenter.org" {rhberg-at-danforthcenter.org} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Rachel, } } The paper by Biggs (1985, Stain Tech. 60: 299-304) differentiates } suberin from lignin by assaying for quenching of lignin } autofluorescence in suberin via staining with Sudan dyes. This worked } for us in study of Casuarina root nodule infected cells, so I can } vouch for its efficacy. } } Cheers, } } Howard } } R. Howard Berg, Ph.D. } Director, Integrated Microscopy Facility } Danforth Plant Science Center } 975 N. Warson Rd. } St. Louis, MO 63132 } } ph 314-587-1261 fx 314-587-1361 cell 314-378-2409 } rhberg-at-danforthcenter.org www.danforthcenter.org } visit this educational resource: http://www.danforthcenter.org/Cells/ } } } } } } } } On Aug 13, 2008, at 9:21 AM, ecoagripolicy-at-gmail.com wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying } } please copy both ecoagripolicy-at-gmail.com as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: ecoagripolicy-at-gmail.com } } Name: Rachel } } } } Title-Subject: [Filtered] Suberin- lignin } } } } Question: Hi all, } } I want to localise and/or differentiate the arrangement of lignin and } } suberin in plant root cell walls. I have used bright field and } } fluorescence microscopy to confirm the presence of lignin and } } suberin. But am not sure which technique will be more appropriate to } } give me more information on the positioning/arrangment of suberin and } } lignin? } } Regards, } } Rachel. } } } } Login Host: 130.95.128.51 } } --------------------------------------------------------------------------- } } } } ==============================Original } } Headers============================== } } 5, 11 -- From zaluzec-at-microscopy.com Wed Aug 13 09:19:26 2008 } } 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id m7DEJPNq013092 } } 5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:19:26 } } -0500 } } 5, 11 -- Mime-Version: 1.0 } } 5, 11 -- Message-Id: {p06240800c4c89dd94be3-at-[206.69.208.22]} } } 5, 11 -- Date: Wed, 13 Aug 2008 09:19:24 -0500 } } 5, 11 -- To: microscopy-at-microscopy.com } } 5, 11 -- From: ecoagripolicy-at-gmail.com (by way of } } MicroscopyListserver) } } 5, 11 -- Subject: viaWWW: Suberin- lignin } } 5, 11 -- Content-Type: text/plain; charset="us-ascii" ; } } format="flowed" } } ==============================End of - } } Headers============================== } } } ==============================Original Headers============================== } 15, 24 -- From RHBerg-at-danforthcenter.org Wed Aug 13 09:59:23 2008 } 15, 24 -- Received: from spm1.ddpsc.org (spm1.ddpsc.org [65.254.111.26]) } 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m7DExMOG027462 } 15, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:59:23 -0500 } 15, 24 -- Received: from spm1.ddpsc.org (127.0.0.1) by spm1.ddpsc.org (MlfMTA } v3.2r9) id hkbrcm0171sk for {Microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 } 09:59:22 -0500 (envelope-from {RHBerg-at-danforthcenter.org} ) } 15, 24 -- Received: from mail1.ddpsc.org ([10.101.0.40]) } 15, 24 -- by spm1.ddpsc.org (SonicWALL 6.1.0.9597) } 15, 24 -- with ESMTP; Wed, 13 Aug 2008 09:59:22 -0500 } 15, 24 -- Received: from [10.14.0.126] ([10.14.0.126]) by mail1.ddpsc.org with } Microsoft SMTPSVC(6.0.3790.3959); } 15, 24 -- Wed, 13 Aug 2008 09:59:22 -0500 } 15, 24 -- Message-Id: } {01060D5E-F697-462C-9561-8EDFAF4E8E7C-at-danforthcenter.org} } 15, 24 -- From: Howard Berg {rhberg-at-danforthcenter.org} } 15, 24 -- To: ecoagripolicy-at-gmail.com, Microscopy-at-microscopy.com } 15, 24 -- In-Reply-To: {200808131421.m7DELbTA015439-at-ns.microscopy.com} } 15, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 15, 24 -- Content-Transfer-Encoding: 7bit } 15, 24 -- Mime-Version: 1.0 (Apple Message framework v926) } 15, 24 -- Subject: Re: [Microscopy] viaWWW: Suberin- lignin } 15, 24 -- Date: Wed, 13 Aug 2008 09:59:22 -0500 } 15, 24 -- References: {200808131421.m7DELbTA015439-at-ns.microscopy.com} } 15, 24 -- X-Mailer: Apple Mail (2.926) } 15, 24 -- X-OriginalArrivalTime: 13 Aug 2008 14:59:22.0354 (UTC) } FILETIME=[2B7A4520:01C8FD55] } 15, 24 -- X-Mlf-Version: 6.1.0.9597 } 15, 24 -- X-Mlf-UniqueId: o200808131459220062092 } ==============================End of - Headers==============================
Biggs used Sudan black B to quench suberin fluorescence and used phoroglucinol HCL to quench lignin autofluorescence enabling good differentiation of the two even when they are localised quite close to each other. We have used this technique extensively in our studies of fruit skins - Another reference is Biggs, 1987, Phytopathology 77:718-725
Ian
Please note new phone numbers
Ian Hallett Sensory and Consumer Science - Microscopy HortResearch, Mt Albert Research Centre Private Bag 92 169, Auckland Mail Centre Auckland 1142, New Zealand Phone: +64-9-925 7027 Fax: +64-9-925 7001
-----Original Message----- X-from: rhberg-at-danforthcenter.org [mailto:rhberg-at-danforthcenter.org] Sent: Thursday, 14 August 2008 3:01 a.m. To: Ian Hallett
Rachel,
The paper by Biggs (1985, Stain Tech. 60: 299-304) differentiates suberin from lignin by assaying for quenching of lignin autofluorescence in suberin via staining with Sudan dyes. This worked for us in study of Casuarina root nodule infected cells, so I can vouch for its efficacy.
Cheers,
Howard
R. Howard Berg, Ph.D. Director, Integrated Microscopy Facility Danforth Plant Science Center 975 N. Warson Rd. St. Louis, MO 63132
ph 314-587-1261 fx 314-587-1361 cell 314-378-2409 rhberg-at-danforthcenter.org www.danforthcenter.org visit this educational resource: http://www.danforthcenter.org/Cells/
On Aug 13, 2008, at 9:21 AM, ecoagripolicy-at-gmail.com wrote:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } ------------------------------------------------------------------------ --- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both ecoagripolicy-at-gmail.com as well as the } MIcroscopy Listserver } ------------------------------------------------------------------------ --- } } Email: ecoagripolicy-at-gmail.com } Name: Rachel } } Title-Subject: [Filtered] Suberin- lignin } } Question: Hi all, } I want to localise and/or differentiate the arrangement of lignin and } suberin in plant root cell walls. I have used bright field and } fluorescence microscopy to confirm the presence of lignin and } suberin. But am not sure which technique will be more appropriate to } give me more information on the positioning/arrangment of suberin and } lignin? } Regards, } Rachel. } } Login Host: 130.95.128.51 } ------------------------------------------------------------------------ --- } } ==============================Original } Headers============================== } 5, 11 -- From zaluzec-at-microscopy.com Wed Aug 13 09:19:26 2008 } 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m7DEJPNq013092 } 5, 11 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:19:26 } -0500 } 5, 11 -- Mime-Version: 1.0 } 5, 11 -- Message-Id: {p06240800c4c89dd94be3-at-[206.69.208.22]} } 5, 11 -- Date: Wed, 13 Aug 2008 09:19:24 -0500 } 5, 11 -- To: microscopy-at-microscopy.com } 5, 11 -- From: ecoagripolicy-at-gmail.com (by way of } MicroscopyListserver) } 5, 11 -- Subject: viaWWW: Suberin- lignin } 5, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 15, 24 -- From RHBerg-at-danforthcenter.org Wed Aug 13 09:59:23 2008 15, 24 -- Received: from spm1.ddpsc.org (spm1.ddpsc.org [65.254.111.26]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7DExMOG027462 15, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:59:23 -0500 15, 24 -- Received: from spm1.ddpsc.org (127.0.0.1) by spm1.ddpsc.org (MlfMTA v3.2r9) id hkbrcm0171sk for {Microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 09:59:22 -0500 (envelope-from {RHBerg-at-danforthcenter.org} ) 15, 24 -- Received: from mail1.ddpsc.org ([10.101.0.40]) 15, 24 -- by spm1.ddpsc.org (SonicWALL 6.1.0.9597) 15, 24 -- with ESMTP; Wed, 13 Aug 2008 09:59:22 -0500 15, 24 -- Received: from [10.14.0.126] ([10.14.0.126]) by mail1.ddpsc.org with Microsoft SMTPSVC(6.0.3790.3959); 15, 24 -- Wed, 13 Aug 2008 09:59:22 -0500 15, 24 -- Message-Id: {01060D5E-F697-462C-9561-8EDFAF4E8E7C-at-danforthcenter.org} 15, 24 -- From: Howard Berg {rhberg-at-danforthcenter.org} 15, 24 -- To: ecoagripolicy-at-gmail.com, Microscopy-at-microscopy.com 15, 24 -- In-Reply-To: {200808131421.m7DELbTA015439-at-ns.microscopy.com} 15, 24 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 15, 24 -- Content-Transfer-Encoding: 7bit 15, 24 -- Mime-Version: 1.0 (Apple Message framework v926) 15, 24 -- Subject: Re: [Microscopy] viaWWW: Suberin- lignin 15, 24 -- Date: Wed, 13 Aug 2008 09:59:22 -0500 15, 24 -- References: {200808131421.m7DELbTA015439-at-ns.microscopy.com} 15, 24 -- X-Mailer: Apple Mail (2.926) 15, 24 -- X-OriginalArrivalTime: 13 Aug 2008 14:59:22.0354 (UTC) FILETIME=[2B7A4520:01C8FD55] 15, 24 -- X-Mlf-Version: 6.1.0.9597 15, 24 -- X-Mlf-UniqueId: o200808131459220062092 ==============================End of - Headers==============================
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==============================Original Headers============================== 26, 26 -- From IHallett-at-hortresearch.co.nz Wed Aug 13 16:33:39 2008 26, 26 -- Received: from hortresearch.co.nz (mscan.hortresearch.co.nz [202.36.134.15]) 26, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7DLXbmP014129 26, 26 -- for {microscopy-at-microscopy.com} ; Wed, 13 Aug 2008 16:33:38 -0500 26, 26 -- X-TM-IMSS-Message-ID: {2933c270002f0abd-at-hortresearch.co.nz} 26, 26 -- Received: from aklexf01.hort.net.nz ([10.16.1.14]) by hortresearch.co.nz ([192.168.8.15]) with SMTP (TREND IMSS SMTP Service 7.0) id 2933c270002f0abd for {IHallett-at-hortresearch.co.nz} ; Thu, 14 Aug 2008 09:34:29 +1200 26, 26 -- Received: from AKLEXB01.hort.net.nz ([10.16.1.15]) by aklexf01.hort.net.nz with Microsoft SMTPSVC(6.0.3790.3959); 26, 26 -- Thu, 14 Aug 2008 09:33:33 +1200 26, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 26 -- Content-class: urn:content-classes:message 26, 26 -- MIME-Version: 1.0 26, 26 -- Content-Type: text/plain; 26, 26 -- charset="us-ascii" 26, 26 -- Subject: RE: [Microscopy] Re: viaWWW: Suberin- lignin 26, 26 -- Date: Thu, 14 Aug 2008 09:33:32 +1200 26, 26 -- Message-ID: {D3BAD63C088F3C48AEB385E881359F8705061E28-at-AKLEXB01.hort.net.nz} 26, 26 -- In-Reply-To: {200808131501.m7DF1IiC030976-at-ns.microscopy.com} 26, 26 -- X-MS-Has-Attach: 26, 26 -- X-MS-TNEF-Correlator: 26, 26 -- Thread-Topic: [Microscopy] Re: viaWWW: Suberin- lignin 26, 26 -- Thread-Index: Acj9VXOsu7NUAWWHRCevCzFxmSbPlwANcO6A 26, 26 -- From: "Ian Hallett" {IHallett-at-hortresearch.co.nz} 26, 26 -- To: {ecoagripolicy-at-gmail.com} , {microscopy-at-microscopy.com} 26, 26 -- X-OriginalArrivalTime: 13 Aug 2008 21:33:33.0782 (UTC) FILETIME=[3CD3C760:01C8FD8C] 26, 26 -- Content-Transfer-Encoding: 8bit 26, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7DLXbmP014129 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Metallographic and EBSD Sample Prep
Question: I recently purchased an EBSD system from EDAX, and in addition to learning how to use the system, am tackling the sample prep issues. I will be getting the Buehler Vibratory Polisher, and also want a semi-automated sample polisher. I am considering the Ecomet/Automet 250 from Buehler, or the MetPrep 3 from Allied High Tech.
The polisher will not be heavily used, primarily by grad students for special projects, so either of these polishers should be suitable for our needs Price is similar. But, I am concerned about reliability, and vendor support. I would appreciate any opinions or feedback that you can share.
Thanks! Anastasia Micheals SEM Facility Manager Department of Chemical and Materials Engineering E-385 San Jose State University One Washington Square San Jose, CA 95192-0082
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eric-at-unquantum.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eric-at-unquantum.net Name: Eric Reiter
Organization: Unquantum
Title-Subject: [Filtered] Etec Omniscan manual
Question: Please; We need a manual for an Etec Omniscan SEM to restore its operation. Thank You
Eric, I might be able to provide scanned copies of the user's and service manuals on CD and a set of schematics, but I have no idea where they might be going. Overseas can get very expensive. Where are you located?
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: eric-at-unquantum.net [mailto:eric-at-unquantum.net] Sent: Wednesday, August 13, 2008 7:30 PM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eric-at-unquantum.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eric-at-unquantum.net Name: Eric Reiter
Organization: Unquantum
Title-Subject: [Filtered] Etec Omniscan manual
Question: Please; We need a manual for an Etec Omniscan SEM to restore its operation. Thank You
One of our customers in Canada has just converted his camera system and has a leftover box of 4489 Kodak film. Rather than toss it Michael would prefer to donate the film to someone in Canada as it is a bit expensive to return it to the U.S.
If anyone would like this film please contact Michael Schoel at schoel-at-ucalgary.ca
Disclaimer: Ladd Research sells a wide variety of microscopy and lab products and equipment including film and photography supplies.
At 05:50 PM 8/13/2008, you wrote: } Att. John Arnot } Hi John, } Re our phone conversation: I have a sealed box of 4489 film, } purchased in January and stored in a fridge, that I am will to } donate to anyone (preferably in Canada) } } Cheers } Michael } -- } } _____________________________ } W. Michael Schoel PhD } Research Associate } Department of Cell Biology & Anatomy } Faculty of Medicine, University of Calgary } 3330 Hospital Drive, N.W. } Calgary, Alberta, Canada T2N 4N1 } Phone: Office (403) 220-3662 } Lab (403) 220-6674 } E-mail: schoel-at-ucalgary.ca
==============================Original Headers============================== 16, 27 -- From jd-at-laddresearch.com Thu Aug 14 12:11:23 2008 16, 27 -- Received: from cutlass.electric.net (cutlass.electric.net [72.35.23.15]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7EHBNvw013952 16, 27 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2008 12:11:23 -0500 16, 27 -- Received: from 1KTgM5-0000Uo-TH by cutlass.electric.net with emc1-ok (Exim 4.67) 16, 27 -- (envelope-from {jd-at-laddresearch.com} ) 16, 27 -- id 1KTgM6-0000W9-TX; Thu, 14 Aug 2008 10:11:22 -0700 16, 27 -- Received: by emcmailer; Thu, 14 Aug 2008 10:11:22 -0700 16, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 16, 27 -- by cutlass.electric.net with esmtps (TLSv1:AES256-SHA:256) 16, 27 -- (Exim 4.67) 16, 27 -- (envelope-from {jd-at-laddresearch.com} ) 16, 27 -- id 1KTgM5-0000Uo-TH; Thu, 14 Aug 2008 10:11:21 -0700 16, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 16, 27 -- Date: Thu, 14 Aug 2008 13:11:16 -0400 16, 27 -- To: Schoel {schoel-at-ucalgary.ca} 16, 27 -- From: Ladd Research {jd-at-laddresearch.com} 16, 27 -- Subject: Re: EM Film to give away 16, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 16, 27 -- In-Reply-To: {48A3570A.4090501-at-ucalgary.ca} 16, 27 -- References: {48A3570A.4090501-at-ucalgary.ca} 16, 27 -- Mime-Version: 1.0 16, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 16, 27 -- X-Outbound-IP: 216.204.198.170 16, 27 -- X-Env-From: jd-at-laddresearch.com 16, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 16, 27 -- Message-Id: {E1KTgM6-0000W9-TX-at-cutlass.electric.net} ==============================End of - Headers==============================
Zeiss updated/modified their TIF file headers to include much more conditions info. This happened in the last two SmartSEM versions of 5.02.
The problem shows up when moving a TIF file from the SEM to some other PC. It will usually crash Explorer and identify msvcr80.dll as the offending module. Windows Computer Management Event Viewer (Applications) will report the same info.
The solution identified by Zeiss is to get the latest SmartTIFF app from Zeiss. This fixes the problem. I've tried this on multiple systems and it works fine.
Hope this helps.
gary g.
==============================Original Headers============================== 7, 17 -- From gary-at-gaugler.com Thu Aug 14 13:53:29 2008 7, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 7, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7EIrSJT031207 7, 17 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2008 13:53:28 -0500 7, 17 -- Message-Id: {200808141853.m7EIrSJT031207-at-ns.microscopy.com} 7, 17 -- Received: (qmail 5640 invoked from network); 14 Aug 2008 11:53:27 -0700 7, 17 -- Received: by simscan 1.1.0 ppid: 5637, pid: 5638, t: 0.1741s 7, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 7, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 7, 17 -- by smtp2 with SMTP; 14 Aug 2008 11:53:27 -0700 7, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 17 -- Date: Thu, 14 Aug 2008 11:53:19 -0700 7, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 7, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 7, 17 -- Subject: Zeiss TIF viewing info 7, 17 -- Mime-Version: 1.0 7, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3724DC3 ==============================End of - Headers==============================
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Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton Universitiy
Title-Subject: [Filtered] Making Support Films for Slotted Grids
Question: I am trying to make my own 1 x 2mm slotted grids with a support film. It's getting quite expensive to be buying them. I have tried a few things (1% formvar, 1% nitrocellulose , both with carbon) but they do not seem to be stable enough. Does anyone have any "special recipe" they might want to pass on?
Thanks so much! Peggy
Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher-at-princeton.edu
These days, I find that 1% Formvar or Butvar is just not strong enough. Try 2-3%, instead. Nitrocellulose definitely should be at least 2%. In addition, I always lay down a thin carbon film if I notice that the films are breaking or drifting. Carbon deposition can be done either right after making the windows OR, even better, after the stained sections are on the films. Then, they are rock solid and never prone to charging.
JB
} } Email: mbisher-at-princeton.edu } Name: Margaret Bisher } } Organization: Princeton Universitiy } } Title-Subject: [Filtered] Making Support Films for Slotted Grids } } Question: I am trying to make my own 1 x 2mm slotted grids with a } support film. It's getting quite expensive to be buying them. I have } tried a few things (1% formvar, 1% nitrocellulose , both with carbon) } but they do not seem to be stable enough. Does anyone have any } "special recipe" they might want to pass on? } } Thanks so much! Peggy } } Margaret E. Bisher } Electron Microscopy & Histology Core Facility Manager } Department of Molecular Biology } Princeton University } Moffett Laboratory, Room 113 } Princeton, New Jersey } Office: (609) 258-7026 } Fax: (609) 258-8468 } mbisher-at-princeton.edu
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
You may have some luck by making a fresh solution from the resin. I had some issues with an older solution and was informed that light causes the diclhloroethane solvent to form HCl. This damages the formvar polymer. I made a fresh solution and kept it in a dark bottle in dark cabinet and the solution lasted well for a couple of years. (I don't use it much.) This is an 0.25% solution on 200 mesh grids.
Good luck , Henk
At 03:28 PM 08/14/08, you wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 9, 26 -- From colijn.1-at-osu.edu Thu Aug 14 16:36:34 2008 9, 26 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7ELaYh3013687 9, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Aug 2008 16:36:34 -0500 9, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31224) 9, 26 -- id {01MYCV67G0748XZN1T-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 9, 26 -- Thu, 14 Aug 2008 17:36:32 -0400 (EDT) 9, 26 -- Received: from HOC1.ecr6.ohio-state.edu 9, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 9, 26 -- (PMDF V6.3-x18 #31224) 9, 26 -- with ESMTPA id {01MYCV62FGHC8X3JAQ-at-ecr6.ohio-state.edu} ; Thu, 9, 26 -- 14 Aug 2008 17:36:25 -0400 (EDT) 9, 26 -- Date: Thu, 14 Aug 2008 17:36:25 -0400 9, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 26 -- Subject: Re: [Microscopy] viaWWW: Making Support Films for Slotted Grids 9, 26 -- In-reply-to: {200808141928.m7EJSUaZ017070-at-ns.microscopy.com} 9, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 26 -- To: mbisher-at-princeton.edu 9, 26 -- Cc: Microscopy-at-microscopy.com 9, 26 -- Message-id: {7.0.1.0.2.20080814173050.035e4eb8-at-osu.edu} 9, 26 -- MIME-version: 1.0 9, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 9, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 26 -- References: {200808141928.m7EJSUaZ017070-at-ns.microscopy.com} ==============================End of - Headers==============================
On Aug 14, 2008, at 1:24 PM, bozzola-at-siu.edu wrote:
} These days, I find that 1% Formvar or Butvar is just not strong } enough. Try 2-3%, instead. Nitrocellulose definitely should be at } least 2%. In addition, I always lay down a thin carbon film if I } notice that the films are breaking or drifting. Carbon deposition can } be done either right after making the windows OR, even better, after } the stained sections are on the films. Then, they are rock solid and } never prone to charging.
Dear John & Margaret, I have had only limited experience with slot grids, but those I have prepared I made the film out of 0.5% formvar. It is important to use freshly prepared formvar, since the films seem to get more brittle as the formvar solution sits. I always coat with C after picking up the formvar film on the grid, and I also coat again with C after the sections have been placed on the grid. Formvar is very susceptible to charging, so never look at the grid without having the objective aperture in place--the electrons backscattered from the Pt will neutralize the charge build-up due to loss of electrons from secondary electron production. The only time you should disregard this rule is when you are scanning the grid at low mag, and then you should use a high spot size number and widely spread beam--the lowest possible dose rate. After looking at sections at ~100 x, I would put in the aperture and go immediately to the lowest magnification in M or SA mode (~1000x on my instruments). Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Aug 14 19:03:36 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7F03a3Y012944 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2008 19:03:36 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 6A08B328009 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2008 17:03:36 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 0BB6D2E504E2 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 14 Aug 2008 17:03:34 -0700 (PDT) 6, 22 -- Message-Id: {0880F737-CB46-4278-A4CD-0772FB917DD2-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200808142024.m7EKOuZD030273-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v926) 6, 22 -- Subject: Re: [Microscopy] Re: Making Support Films for Slotted Grids 6, 22 -- Date: Thu, 14 Aug 2008 17:03:34 -0700 6, 22 -- References: {200808142024.m7EKOuZD030273-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.926) ==============================End of - Headers==============================
The guy from our FEI representative came last Monday. And the problem really was at the stage power supply. We already order a new one. I just think it would be interesting to let you (and the mailing list) know the issue was solved.
Thanks for the help.
Regards,
On Thu, 2008-07-31 at 11:29 -0700, Gursky, Richard wrote: } Hello Filipi, } } You may want to contact your local FEI Service organization, but in the mean time I hope this helps. } } Regards, } Ritch } Richard Gursky } Product Marketing Engineer } Sr. Applications Engineer } FEI Company } 5350 NE Dawson Creek Drive } Hillsboro, OR 97124 } Office: 503-726-1284 } Cell: 503-298-1865 } Please Note New Email Address: } Richard.Gursky-at-FEI.com } } } -----Original Message----- } From: TSG SEM SDB NA } Sent: Thursday, July 31, 2008 11:17 AM } To: Gursky, Richard; TSG SEM SDB NA } Subject: RE: [Microscopy] Phillips XL30 } } This is most likely the Stage Power 5V supply, part # 5322 695 15934, this part is more common to fail and the CMXX bus error supports this problem. I cannot say with 100% confidence but maybe 90%. They will need to get a service engineer on site to be sure. } } -Derek Barrington } } -----Original Message----- } From: Gursky, Richard } Sent: Thursday, July 31, 2008 10:13 AM } To: TSG SEM SDB NA } Subject: FW: [Microscopy] Phillips XL30 } } Can someone help this person? } } Regards, } Ritch } Richard Gursky } Product Marketing Engineer } Sr. Applications Engineer } FEI Company } 5350 NE Dawson Creek Drive } Hillsboro, OR 97124 } Office: 503-726-1284 } Cell: 503-298-1865 } Please Note New Email Address: } Richard.Gursky-at-FEI.com } } -----Original Message----- } From: filipi-at-pucrs.br [mailto:filipi-at-pucrs.br] } Sent: Thursday, July 31, 2008 8:06 AM } To: Gursky, Richard } Subject: [Microscopy] Phillips XL30 } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi folks, } } Does anyone in this list have a Philips XL30 SEM? } } We have toke off the penning for cleaning, and after put } it back, we could not start up the SEM properly anymore. } } When starting up the server, it always stop at the } stage initialization and show the following error: } } "SMCB driver - DirectSend: Put CMxx I/O bus hardware } problem 2nd address (c128c01f)." } } We can make vacuum, and imaging. The only thing which } doesn't work is the stage control. } } The question is: } What the penning cleaning procedure has to do with the stage? } } We have been in contact with local Philips representative, } and they told us this could be a cabling issue. } } We just want to figure out why the penning cleaning procedure } could damage anything on stage control hardware. } } Regards, -- Filipi Vianna IDEIA/PUCRS Research and Development Institute +55 51 33203525 ext. 7794 http://www.pucrs.br/ideia
==============================Original Headers============================== 6, 20 -- From filipi-at-pucrs.br Fri Aug 15 06:51:40 2008 6, 20 -- Received: from rigel.pucrs.br (rigel.pucrs.br [201.54.140.13]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7FBpenl017492 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Aug 2008 06:51:40 -0500 6, 20 -- Received: from [10.30.46.17] (unknown [10.30.46.17]) 6, 20 -- by rigel.pucrs.br (Postfix) with ESMTP id D5B6D2626F; 6, 20 -- Fri, 15 Aug 2008 08:51:38 -0300 (BRT) 6, 20 -- Subject: Re: FW: [Microscopy] Phillips XL30 6, 20 -- From: Filipi Vianna {filipi-at-pucrs.br} 6, 20 -- To: "Gursky, Richard" {Richard.Gursky-at-fei.com} 6, 20 -- Cc: Microscopy {Microscopy-at-microscopy.com} 6, 20 -- In-Reply-To: {00948E5E66F1374FB9284A7B78138EB813269DA5-at-hlexc03.w2k.feico.com} 6, 20 -- References: {00948E5E66F1374FB9284A7B78138EB813269DA5-at-hlexc03.w2k.feico.com} 6, 20 -- Content-Type: text/plain; charset=UTF-8 6, 20 -- Organization: =?ISO-8859-1?Q?ID=C9IA-PUCRS?= 6, 20 -- Date: Fri, 15 Aug 2008 08:52:20 -0300 6, 20 -- Message-Id: {1218801140.19347.9.camel-at-filipi-desktop} 6, 20 -- Mime-Version: 1.0 6, 20 -- X-Mailer: Evolution 2.22.3.1 6, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Check out the $10 microscope on a chip at http://www.pnas.org/content/early/2008/07/25/0804612105.full.pdf+html
 Lensless high-resolution on-chip optofluidic microscopes for Caenorhabditis elegans and cell imaging Xiquan Cui*,†, Lap Man Lee†,‡, Xin Heng*, Weiwei Zhong§, Paul W. Sternberg§, Demetri Psaltis*,¶, and Changhuei Yang*,‡,‖
Thomas E. Phillips, Ph.D. Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall Biological Sciences University of Missouri Columbia, MO 65211-7400 573-882-4712 (voice) 573-882-0123 (fax)
==============================Original Headers============================== 7, 23 -- From PhillipsT-at-missouri.edu Fri Aug 15 08:49:34 2008 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7FDnYQW002674 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 15 Aug 2008 08:49:34 -0500 7, 23 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Fri, 15 Aug 2008 08:49:33 -0500 7, 23 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="utf-8" 7, 23 -- Subject: lensless microscope on a chip 7, 23 -- Date: Fri, 15 Aug 2008 08:49:36 -0500 7, 23 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD04AC438B-at-UM-XMAIL06.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- thread-topic: lensless microscope on a chip 7, 23 -- thread-index: Acj+3cFFjUPp/AxdQkqZmJrPlBdOWg== 7, 23 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 15 Aug 2008 13:49:34.0059 (UTC) FILETIME=[BFE2ABB0:01C8FEDD] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id m7FDnYQW002674 ==============================End of - Headers==============================
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Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton Universitiy
Title-Subject: [Filtered] Making Support Films for Slotted Grids
Question: I am trying to make my own 1 x 2mm slotted grids with a support film. It's getting quite expensive to be buying them. I have tried a few things (1% formvar, 1% nitrocellulose , both with carbon) but they do not seem to be stable enough. Does anyone have any "special recipe" they might want to pass on?
Thanks so much! Peggy
Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher-at-princeton.edu
We have an old Balzer's MED 010 coating system, and I am trying to either find the original glow discharge attachment that fits in the vacuum chamber, or a detailed picture and/or schematic of this part so that I can have our machine shop guy make something similar. The Balzer's part number is BU 007 284 -T if that helps.
If anyone knows where I could find this part, or if you can tell me a little more about how it works so I can make something similar, that would be a huge help! Our even older coating system which we use for glow discharging has blown a turbo pump and we would just like to get rid of it and use the MED for carbon coating/glow discharging.
Thanks, -- Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 7, 29 -- From bnross-at-interchange.ubc.ca Mon Aug 18 11:52:42 2008 7, 29 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7IGqf9R005712 7, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Aug 2008 11:52:41 -0500 7, 29 -- X-Ubc-Received: from mr5.mail-relay.ubc.ca (localhost [127.0.0.1]) 7, 29 -- by localhost (Postfix) with SMTP id 70AFC114FA 7, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Aug 2008 09:52:41 -0700 (PDT) 7, 29 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 7, 29 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP 7, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Aug 2008 09:52:40 -0700 (PDT) 7, 29 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14]) 7, 29 -- by smtp.interchange.ubc.ca 7, 29 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 7, 29 -- with ESMTP id {0K5T00A5E2VRH1-at-smtp.interchange.ubc.ca} for 7, 29 -- Microscopy-at-microscopy.com; Mon, 18 Aug 2008 09:52:40 -0700 (PDT) 7, 29 -- Date: Mon, 18 Aug 2008 09:52:39 -0700 (PDT) 7, 29 -- From: Bradford Ross {bnross-at-interchange.ubc.ca} 7, 29 -- Subject: Balzers MED 010 Glow Discharge Device 7, 29 -- To: Microscopy-at-microscopy.com 7, 29 -- Message-id: {27644324.2191219078359871.JavaMail.myubc2-at-handel.my.ubc.ca} 7, 29 -- MIME-version: 1.0 7, 29 -- X-Mailer: uPortal WEB email client 3.0 7, 29 -- Content-type: text/plain; charset=us-ascii 7, 29 -- Content-transfer-encoding: 7bit 7, 29 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.8.18.163719 7, 29 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 7, 29 -- X-PerlMx-Spam: Probability=7%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_1000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_900_999 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 7, 29 -- X-Spam-Level: 7, 29 -- X-Spam-Flag: No ==============================End of - Headers==============================
The College of Microscopy, located in Westmont, IL, is offering the following electron microscopy short courses:
September 16 to 18 - Transmission Electron Microscopy
October 6 to 10 - Scanning Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Regards,
Elaine
Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 12, 23 -- From eschumacher-at-mccrone.com Mon Aug 18 12:48:16 2008 12, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7IHmGhP020927 12, 23 -- for {microscopy-at-microscopy.com} ; Mon, 18 Aug 2008 12:48:16 -0500 12, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 12, 23 -- Mon, 18 Aug 2008 12:48:16 -0500 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="US-ASCII" 12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 23 -- Subject: Short Course Announcement: TEM and SEM 12, 23 -- Date: Mon, 18 Aug 2008 12:48:14 -0500 12, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150E076-at-MCCRONEMSG.tmg.mccrone.com} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: Short Course Announcement: TEM and SEM 12, 23 -- Thread-Index: AckBWpaeECdbtelASp+01wfrgWznsA== 12, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 12, 23 -- To: {microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 18 Aug 2008 17:48:16.0476 (UTC) FILETIME=[97F351C0:01C9015A] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7IHmGhP020927 ==============================End of - Headers==============================
Thank you very much for your excellent feedback re: Service Engineers for Leica Ultramicrotomes.
I have another question... has anyone tried out the Diatome Ultra Sonic Oscillating Diamond Knife? How do you like it? Does it give you consistent results? Pros and Cons?
Thank you in advance, Have a good afternoon, Virginia -- Virginia Tanner Crocker Biologist NIH, NINDS EM Facility MSC4477 Bldg. 49, Room 3C58 49 Convent Drive Bethesda, MD 20892 301-402-1568 301-480-1485 (fax) crockerv-at-ninds.nih.gov (email)
==============================Original Headers============================== 3, 17 -- From crockerv-at-ninds.nih.gov Mon Aug 18 14:20:58 2008 3, 17 -- Received: from nihrelayxway6.hub.nih.gov (nihrelayxway6.hub.nih.gov [128.231.90.100]) 3, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7IJKw1I005701 3, 17 -- for {MICROSCOPY-at-sparc5.microscopy.com} ; Mon, 18 Aug 2008 14:20:58 -0500 3, 17 -- X-IronPortListener: NIH_Relay 3, 17 -- X-SBRS: None 3, 17 -- X-IronPort-AV: E=Sophos;i="4.32,229,1217822400"; 3, 17 -- d="scan'208";a="45703460" 3, 17 -- Received: from unknown (HELO [165.112.21.103]) ([128.231.90.83]) 3, 17 -- by nihrelayxway6.hub.nih.gov with ESMTP; 18 Aug 2008 15:11:44 -0400 3, 17 -- Mime-Version: 1.0 3, 17 -- Message-Id: {p06230908c4cf77bec9ce-at-[165.112.21.103]} 3, 17 -- Date: Mon, 18 Aug 2008 15:11:47 -0400 3, 17 -- To: MICROSCOPY-at-ns.microscopy.com 3, 17 -- From: Virginia Crocker {crockerv-at-ninds.nih.gov} 3, 17 -- Subject: A new question re: Diatome Ultra Sonic Oscillating Diamond Knife 3, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: Victoria.allen-at-serco.com Name: Vicky Allen
Question: At Serco we have the power supply section of a struers polectrol electropolishing kit. However, we are missing the part which the specimen to be electropolished sits in. We have contacted Struers but as it is an old system they no longer supply these parts. Does anyone have any spare parts for a polectrol electropolisher?
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Email: watson-at-wi.mit.edu Name: Nicki Watson
Organization: Whitehead Institute
Title-Subject: [Filtered] SEM Red blood cells
Question: Hi everyone, Does anyone have a good detailed protocol for preparing red blood cells for SEM? Do you filter them? how do you attach them to a substrate that can be carried through the dehydration process and CPD? Thank you in advance for your helpful suggestions.
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: ASU
Title-Subject: [Filtered] cacodylate buffer and immuno-labeling
Question: we are preparing to do a project with immuno-labeling of mouse brain for TEM. For previous standard TEM work on mouse brain we have used cacodylate buffer (with Ca2+ added) and gotten excellent results, but now have questions about arsenic causing problems with antigens. I haven't been able to find anything specifically pertaining to this question. Does anyone know if there are contraindications for the use of cacodylate buffer in samples destined for on-section labeling for TEM?
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Email: danielhc01-at-yahoo.com.mx Name: Daniel Hernandez-Cruz
I read in a previous message someone suggested someone else to use XPSPEAK to use it for spectra deconvolution and related issues.
I started to use the software before I read that message and found if very useful, but i would like to make some change or put some other features.
I tried to contact Prof. Kwok, the person who wrote the program, I the e-mail he has provided is not correct or I dont know what is going on.
I would like to contact him... Does anybody know how I can contact him??
Thanks
Daniel
Daniel Hernandez-Cruz, Ph.D. IPICYT-Advanced Materials Dept. San Luis Potosi, Mexico tel +52-444-834-2000 x7204 daniel.hernandez-at-ipicyt.edu.mx danielhc01-at-yahoo.com.mx
I used one of the first knives of this type in 2004 to cut high pressure frozen yeast in a cryo system at -160. It worked fine for thin sections {100nm but did not help us in our quest to cut 250nm sections. For cryo work I preferred the cryo-platform knife for ease of transferring sections to grids. What type of samples do you have? Larry
crockerv-at-ninds.nih.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thank you very much for your excellent feedback re: Service Engineers } for Leica Ultramicrotomes. } } I have another question... has anyone tried out the Diatome Ultra } Sonic Oscillating Diamond Knife? How do you like it? Does it give } you consistent results? Pros and Cons? } } Thank you in advance, } Have a good afternoon, } Virginia
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 6, 30 -- From Larry.Ackerman-at-ucsf.edu Tue Aug 19 12:10:49 2008 6, 30 -- Received: from emfmcb02.ucsfmedicalcenter.org (EMFMCB02.ucsfmedicalcenter.org [64.54.46.98]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7JHAnvQ009409 6, 30 -- for {microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 12:10:49 -0500 6, 30 -- Received: from 64.54.128.150 by emfmcb02.ucsfmedicalcenter.org with 6, 30 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 6, 30 -- Tue, 19 Aug 2008 10:07:10 -0700 6, 30 -- X-Server-Uuid: 44B63953-633F-4500-B2DA-A3E478718104 6, 30 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 6, 30 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Tue, 19 Aug 6, 30 -- 2008 10:10:42 -0700 6, 30 -- Message-ID: {48AAFE86.9030503-at-ucsf.edu} 6, 30 -- Date: Tue, 19 Aug 2008 10:10:30 -0700 6, 30 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 6, 30 -- Reply-to: larry.ackerman-at-ucsf.edu 6, 30 -- Organization: UCSF, NeuroAnatomy 6, 30 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 6, 30 -- MIME-Version: 1.0 6, 30 -- To: crockerv-at-ninds.nih.gov, microscopy-at-microscopy.com 6, 30 -- Subject: [Microscopy] A new question re: Diatome Ultra Sonic Oscillating 6, 30 -- Diamond Knife 6, 30 -- References: {200808181927.m7IJRXd1016344-at-ns.microscopy.com} 6, 30 -- In-Reply-To: {200808181927.m7IJRXd1016344-at-ns.microscopy.com} 6, 30 -- X-OriginalArrivalTime: 19 Aug 2008 17:10:42.0202 (UTC) 6, 30 -- FILETIME=[82B627A0:01C9021E] 6, 30 -- X-WSS-ID: 64B422342CK667879-01-01 6, 30 -- Content-Type: text/plain; 6, 30 -- charset=iso-8859-1; 6, 30 -- format=flowed 6, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear List, Many years ago I had a protocol for recrystallizing catalase, but no longer. As I remember, one simply dissolves the catalase in a buffer, then changes the ionic strength or pH--I can't remember which--to precipitate the crystals. Can anyone tell me the details or give me a reference? TIA. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Tue Aug 19 13:25:32 2008 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7JIPWI7028664 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 13:25:32 -0500 2, 20 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by fire-doxen-postvirus (Postfix) with ESMTP id 17E69328019 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 11:25:32 -0700 (PDT) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 2, 20 -- by fire-doxen-ssl (Postfix) with ESMTP id 2B03832840F 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 11:25:31 -0700 (PDT) 2, 20 -- Message-Id: {04ED20DF-AFD9-4621-9AEF-980759E44808-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v926) 2, 20 -- Subject: Catalase recrystallization 2, 20 -- Date: Tue, 19 Aug 2008 11:25:30 -0700 2, 20 -- X-Mailer: Apple Mail (2.926) ==============================End of - Headers==============================
Hi Bill: Catalase recrystallization was always a hit or miss thing for me. I found my old and dusty notes from 20 years ago and here it is:
Akey and Edelstein JMB (1983)163:575-612
To dissolve the crystals: 0.47 gm / 100 ml KH2PO4 and 0.814 gm/100 ml Na2PO4 pH 7.4
Then bring the pH back to 5.3 with saturated KH2PO4
I also have an old reference for dialyzing out the crystals. That seemed to produce larger crystals but that was also hit or miss.
Hope that works.
Norm
At 1:26 PM -0500 8/19/08, {tivol-at-caltech.edu} wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
--
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
==============================Original Headers============================== 17, 25 -- From nholson-at-ucsd.edu Tue Aug 19 13:57:40 2008 17, 25 -- Received: from outbound3.ucsd.edu (outbound3.ucsd.edu [132.239.1.207]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7JIvdfs010990 17, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 13:57:39 -0500 17, 25 -- Received: from smtp.ucsd.edu (smtp.ucsd.edu [132.239.1.49]) 17, 25 -- by outbound3.ucsd.edu (8.13.6/8.13.6) with ESMTP id m7JIvcgL068544 17, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK); 17, 25 -- Tue, 19 Aug 2008 11:57:38 -0700 (PDT) 17, 25 -- DomainKey-Signature: a=rsa-sha1; s=2007001; d=ucsd.edu; c=simple; q=dns; 17, 25 -- b=EhtGxixqvhi2G5k/6DnzTE+xKmRBoJE0qVFH7iwuHhuSoo3lS7yTF9jO3CSSeRLpo 17, 25 -- VHpOsD2jXDiO3OgQh7gAw== 17, 25 -- Received: from [132.239.70.186] (patrick-70-186.ucsd.edu [132.239.70.186]) 17, 25 -- by smtp.ucsd.edu (8.13.6/8.13.6) with ESMTP id m7JIvZRW093454; 17, 25 -- Tue, 19 Aug 2008 11:57:37 -0700 (PDT) 17, 25 -- X-Authentication-Warning: smtp.ucsd.edu: Host patrick-70-186.ucsd.edu [132.239.70.186] claimed to be [132.239.70.186] 17, 25 -- Mime-Version: 1.0 17, 25 -- Message-Id: {p06240802c4d0c680a646-at-[132.239.70.186]} 17, 25 -- In-Reply-To: {200808191826.m7JIQjmf030549-at-ns.microscopy.com} 17, 25 -- References: {200808191826.m7JIQjmf030549-at-ns.microscopy.com} 17, 25 -- Date: Tue, 19 Aug 2008 11:57:28 -0700 17, 25 -- To: tivol-at-caltech.edu 17, 25 -- From: Norm Olson {nholson-at-ucsd.edu} 17, 25 -- Subject: Re: [Microscopy] Catalase recrystallization 17, 25 -- Cc: microscopy-at-microscopy.com 17, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: kaszas.1-at-osu.edu Name: Andrea Kaszas
Organization: Ohio State University/OARDC
Title-Subject: [Filtered] Tissue Processor
Question: Dear Listservers,
We are planning to purchase a tissue processor for histology and electron microscopy sample preparation, and are considering the LYNX II Automated Tissue Processor (EMS) or the EMP-5160 Tissue Processor (Boeckeler Instruments, Inc.).
If anyone has experience with any of these two instruments we would appreciate, if you can share it with us.
Thank you.
Andrea Kaszas Research Associate MCIC OSU/OARDC 1680 Madison Ave Wooster OH 44691
It is with a heavy heart that I tell the society that we have lost another great scientist. Dr. Samuel Spicer was internationally known for his work in carbohydrate chemistry and histochemistry. I have enclosed the article from the local paper.
Ex-MUSC professor dies Dr. Samuel Sherman Spicer Jr., internationally known for his work with carbohydrate chemistry and histochemistry and former professor of pathology at the Medical University of South Carolina, died Monday. He was 94. A native of Denver, Spicer was born to Samuel S. Spicer and Eleanor Kirk Spicer. He attended the University of Colorado, where he received his bachelor of science degree in 1936 and his doctor of medicine degree in 1939. Following an internship at the University of Wisconsin Madison General Hospital, he was commissioned assistant surgeon in 1940 with the U.S. Public Health Service, where he worked on narcotics and malaria control. He retired as medical director in 1966.
That same year, while Spicer was chief of the Section on Biophysical Histology at the Laboratory of Experimental Pathology at the National Institutes of Health, he was named professor of pathology at MUSC, resulting in his move to Charleston to join the school's Department of Pathology and Laboratory Medicine. Spicer received an honorary medical degree from Sweden's Linkoping University and an award from the Japanese Society for the Promotion of Science. He published 460 original research papers during his 60¬year career and has been cited more than any other researcher in South Carolina. In 1998, Spicer was awarded an honorary doctor of science degree from MUSC. Spicer is the widower of Gertrude McRae Spicer. He is survived by two sons, Kenneth and Samuel; one daughter, Eleanor; and eight grandchildren.
Nancy M Smythe Department of Otolaryngology Head and Neck Surgery Medical University of South Carolina Charleston, SC 29425
843 792 9587 843 792 0368 Fax
==============================Original Headers============================== 6, 31 -- From smythen-at-musc.edu Tue Aug 19 16:50:00 2008 6, 31 -- Received: from ironp1.musc.edu (ironp1.musc.edu [128.23.34.114]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7JLnxB7014382 6, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 16:50:00 -0500 6, 31 -- X-IronPort-GLBA: NPI-Med 6, 31 -- X-IronPort-AV: E=Sophos;i="4.32,237,1217822400"; 6, 31 -- d="scan'208";a="421374588" 6, 31 -- Received: from exg-hub.musc.edu (exg-hub1.mdc.musc.edu [128.23.1.66]) 6, 31 -- by revere5b.musc.edu (8.13.5/8.13.5) with ESMTP id m7JLnkAk031378 6, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Aug 2008 17:49:46 -0400 6, 31 -- Received: from EXG-HUB3.clinlan.local (128.23.1.188) by exg-hub1.clinlan.local 6, 31 -- (128.23.1.66) with Microsoft SMTP Server (TLS) id 8.1.291.1; Tue, 19 Aug 2008 6, 31 -- 17:49:58 -0400 6, 31 -- Received: from EVS4.clinlan.local ([128.23.180.1]) by EXG-HUB3.clinlan.local 6, 31 -- ([2002:8017:1bc::8017:1bc]) with mapi; Tue, 19 Aug 2008 17:49:57 -0400 6, 31 -- From: "Smythe, Nancy M." {smythen-at-musc.edu} 6, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 31 -- Date: Tue, 19 Aug 2008 17:49:57 -0400 6, 31 -- Subject: Samuel S Spicer, Jr 6, 31 -- Thread-Topic: Samuel S Spicer, Jr 6, 31 -- Thread-Index: AckCRYVwYvXdlrNhTCCIWIHXrgIOIg== 6, 31 -- Message-ID: {BA33F8FA3F2FA544A0D4F75A43176141F0BEE78AC8-at-EVS4.clinlan.local} 6, 31 -- Accept-Language: en-US 6, 31 -- Content-Language: en-US 6, 31 -- X-MS-Has-Attach: 6, 31 -- X-MS-TNEF-Correlator: 6, 31 -- acceptlanguage: en-US 6, 31 -- Content-Type: text/plain; charset="iso-8859-1" 6, 31 -- MIME-Version: 1.0 6, 31 -- Content-Transfer-Encoding: 8bit 6, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7JLnxB7014382 ==============================End of - Headers==============================
We are writing to ask for your opinion on the most significant impact(s) made on life sciences and medicine by x-ray microanalysis. We will compile all responses and present them in an overview lecture - with due acknowledgements of course - at the European Congress of Microscopy being held this year in Aachen, Germany, on September 1 - 5. Your opinions will be greatly appreciated.
With best regards and thank you in advance.
Ann LeFurgey and Peter Ingram
PS If you choose to respond please include the word IMPACT in the Subject line
-- Peter Ingram Sr. Physicist Adj. Professor of Pathology, Duke University Medical Center Box 90319 LaSalle Street Extension DURHAM NC USA 27708-0319
Ann LeFurgey, Ph.D. Associate Research Professor Department of cell Biology Duke University Medical Center and Research Biologist P&LMS (113) 508 Fulton Street DURHAM NC USA 27705
I recently acquired a Bausch & Lomb Dynazoom II metallograph, one of those very compact inverted microcopes used in academic labs where undergraduates can't do too much harm.
This one has an X-Y stage with some mangled brass shim-stock connectors that couple the screw nuts to the translation mechanism.
While I could make 'em myself with a little effort, I'm inquiring whether:
a. Someone on the list knows a source of such repair parts; or b. Someone else on the list is in a similar predicament.
If it's (a) send me a note offline. If it's (b) do the same and I'll make more than the two that I need at no extra cost to the respondent(s).
George Langford, Sc.D. Principal Consultant Amenex Associates, Inc. amenex-at-amenex.com http://www.amenex.com/
==============================Original Headers============================== 7, 24 -- From amenex-at-amenex.com Wed Aug 20 14:54:19 2008 7, 24 -- Received: from c60.cesmail.net (c60.cesmail.net [216.154.195.49]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7KJsJwG010067 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Aug 2008 14:54:19 -0500 7, 24 -- Received: from unknown (HELO epsilon2) ([192.168.1.60]) 7, 24 -- by c60.cesmail.net with ESMTP; 20 Aug 2008 15:54:19 -0400 7, 24 -- Received: from pool-96-227-71-166.phlapa.fios.verizon.net 7, 24 -- (pool-96-227-71-166.phlapa.fios.verizon.net [96.227.71.166]) by 7, 24 -- webmail.spamcop.net (Horde MIME library) with HTTP; Wed, 20 Aug 2008 7, 24 -- 15:54:19 -0400 7, 24 -- Message-ID: {20080820155419.yoebu2wlm5cccs4c-nzrark-at-webmail.spamcop.net} 7, 24 -- Date: Wed, 20 Aug 2008 15:54:19 -0400 7, 24 -- From: "George Langford, Sc.D." {amenex-at-amenex.com} 7, 24 -- To: Microscopy-at-microscopy.com 7, 24 -- Cc: "George Langford, Sc.D." {amenex-at-amenex.com} 7, 24 -- Subject: Parts for a B&L Dynazoom II metallograph 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; 7, 24 -- charset=ISO-8859-1; 7, 24 -- DelSp="Yes"; 7, 24 -- format="flowed" 7, 24 -- Content-Disposition: inline 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.4) ==============================End of - Headers==============================
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Email: Wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College/UPEI
Title-Subject: [Filtered] high resulution mode Hitachi TEM
Question: Hello, I have a user who asked that her samples are scoped in HR mode at 200K. Is there a special aligment, beside a regular aligment done once in a while, of the scope that I have to do before I start scoping? Thanks Dorota
} We are writing to ask for your opinion on the most } significant impact(s) made on life sciences and medicine by } x-ray microanalysis.
Although not associated with the life science or medicine, as well as highly speculative, I thought I'd offer the following anecdotal tidbit ...
I was lucky enough to enter the field of EPMA somwhat late during the period after the NASA moon missions, when there was considerable funding for instrumentation and research projects involving the rock returned on those missions. During the several conferences I attended, I got the very strong impression that rocks were returned to earth only because of what could be gained from EPMA. If you take that speculation only one step further and assume we sent men to the moon for bringing back samples, then we may have never put men on the moon if not for EPMA(!?!)
Food for thought :o) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 6, 29 -- From michael-at-shaffer.net Thu Aug 21 10:02:01 2008 6, 29 -- Received: from smtprelay.hostedemail.com (smtprelay0116.hostedemail.com [216.40.44.116]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7LF21Kx028719 6, 29 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2008 10:02:01 -0500 6, 29 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 6, 29 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id E50B7208350; 6, 29 -- Thu, 21 Aug 2008 15:02:00 +0000 (UTC) 6, 29 -- X-SpamScore: 1 6, 29 -- X-Spam-Summary: 50,0,0,cf896e509a1fe24d,28c13fddcb7c6f1e,michael-at-shaffer.net,,RULES_HIT:10:355:379:539:541:542:599:601:967:988:989:1155:1160:1260:1277:1311:1313:1314:1345:1359:1437:1515:1516:1518:1534:1541:1593:1594:1711:1730:1747:1766:1792:2378:2393:2525:2553:2560:2563:2682:2685:2687:2735:2857:2859:2933:2937:2939:2942:2945:2947:2951:2954:3022:3027:3352:3636:3865:3866:3867:3868:3873:3874:3934:3936:3938:3941:3944:3947:3950:3953:4250:5007:6261:7679:7903:8985:9025,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 6, 29 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 6, 29 -- (Authenticated sender: michael-at-shaffer.net) 6, 29 -- by omf03.hostedemail.com (Postfix) with ESMTP; 6, 29 -- Thu, 21 Aug 2008 15:02:00 +0000 (UTC) 6, 29 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 29 -- To: {p.ingram-at-voice.cellbio.duke.edu} 6, 29 -- Cc: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 29 -- References: {200808201629.m7KGT8S3018032-at-ns.microscopy.com} 6, 29 -- Subject: RE: [Microscopy] Impact of x-ray microanalysis 6, 29 -- Date: Thu, 21 Aug 2008 12:31:59 -0230 6, 29 -- Message-ID: {46A75F547101442F91B9D287880556BE-at-CREAIT.MUN.CA} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-Mailer: Microsoft Office Outlook 11 6, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 6, 29 -- Thread-Index: AckC4d52niSAqMG0SfOkXHsLGnlA7wAu602A 6, 29 -- In-Reply-To: {200808201629.m7KGT8S3018032-at-ns.microscopy.com} 6, 29 -- X-session-marker: 6D69636861656C40736861666665722E6E6574 ==============================End of - Headers==============================
I have a person who wants to negative stain marine cyanobacteria. Some are grown on agar plates and scraped off and others are grown in media. What we would like to know is...
What is the best way to negative stain these baceria? We have used 2% aq. UA and 1% PTA the stains work but not as well as we would like. We let the bugs sit for 5 minutes, wick off the excess and then have applied the stain from any where from 15 seconds to 1 minute.
We are definitely seeing the bacteria and they appear collapsed.
Any suggestions from the great collective mind of the list?
Thanks in advance,
Paula :-)
Paula Sicurello VA Medical Center San Diego Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 11, 24 -- From vapatpxs-at-yahoo.com Thu Aug 21 10:24:40 2008 11, 24 -- Received: from n77.bullet.mail.sp1.yahoo.com (n77.bullet.mail.sp1.yahoo.com [98.136.44.45]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7LFOd4u010866 11, 24 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2008 10:24:40 -0500 11, 24 -- Received: from [216.252.122.218] by n77.bullet.mail.sp1.yahoo.com with NNFMP; 21 Aug 2008 15:24:39 -0000 11, 24 -- Received: from [69.147.84.109] by t3.bullet.sp1.yahoo.com with NNFMP; 21 Aug 2008 15:24:39 -0000 11, 24 -- Received: from [127.0.0.1] by omp207.mail.sp1.yahoo.com with NNFMP; 21 Aug 2008 15:24:39 -0000 11, 24 -- X-Yahoo-Newman-Property: ymail-3 11, 24 -- X-Yahoo-Newman-Id: 647158.90977.bm-at-omp207.mail.sp1.yahoo.com 11, 24 -- Received: (qmail 56686 invoked by uid 60001); 21 Aug 2008 15:17:18 -0000 11, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 24 -- s=s1024; d=yahoo.com; 11, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 11, 24 -- b=h6pviTv/nYyU/HD78g7UFppc9j/1WBVm3tmljDaKGQnaZptntaVznLzMtSOW7TLCi/Yx+oyvR4QMS8Yi3XaKYQuzR8bdvQhjRqOfzCMKQJdI9rJ1srBaQeET/W7YRxULhgrE4tpVHXN4UbFDeXYdok7T98ROf4bYlroCtJbpDWc=; 11, 24 -- Received: from [24.30.157.32] by web46111.mail.sp1.yahoo.com via HTTP; Thu, 21 Aug 2008 08:17:18 PDT 11, 24 -- X-Mailer: YahooMailWebService/0.7.218 11, 24 -- Date: Thu, 21 Aug 2008 08:17:18 -0700 (PDT) 11, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 11, 24 -- Reply-To: vapatpxs-at-yahoo.com 11, 24 -- Subject: Negative stain of cyanobacteria 11, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 11, 24 -- MIME-Version: 1.0 11, 24 -- Content-Type: text/plain; charset=us-ascii 11, 24 -- Message-ID: {479465.43031.qm-at-web46111.mail.sp1.yahoo.com} ==============================End of - Headers==============================
I learned to use the stain to wash off the unattached sample and carrier fluid rather than wicking it off.
Add the staining solution drop-wise (3-4 drops) to the grid with sample holding it at a slight angle with forceps over a dish and let the overflowing drop slip off. Hold the grid flat and let the last drop of stain sit for about a minute on the grid to accomplish the staining then wick the excess stain off slowly. This eliminated a premature drying of the sample.
Hoping you soon get excellent results! Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {vapatpxs-at-yahoo.com} } Reply-To: {vapatpxs-at-yahoo.com} } Date: Thu, 21 Aug 2008 10:28:44 -0500 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] Negative stain of cyanobacteria
} Hello Listers, } } I have a person who wants to negative stain marine cyanobacteria. Some are } grown on agar plates and scraped off and others are grown in media. What we } would like to know is... } } What is the best way to negative stain these baceria? We have used 2% aq. UA } and 1% PTA the stains work but not as well as we would like. We let the bugs } sit for 5 minutes, wick off the excess and then have applied the stain from } any where from 15 seconds to 1 minute. } } We are definitely seeing the bacteria and they appear collapsed. } } Any suggestions from the great collective mind of the list? } } Thanks in advance, } } Paula :-) } } Paula Sicurello } VA Medical Center San Diego } Core Microscope Facility, room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397
==============================Original Headers============================== 10, 27 -- From connellyps-at-nhlbi.nih.gov Thu Aug 21 12:33:07 2008 10, 27 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7LHX67M031845 10, 27 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2008 12:33:06 -0500 10, 27 -- X-IronPortListener: Outbound_SMTP 10, 27 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) 10, 27 -- by nihxway3out.hub.nih.gov with ESMTP; 21 Aug 2008 13:23:40 -0400 10, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); 10, 27 -- Thu, 21 Aug 2008 13:23:36 -0400 10, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.169]) with Microsoft Exchange Server HTTP-DAV ; 10, 27 -- Thu, 21 Aug 2008 17:23:36 +0000 10, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 27 -- Date: Thu, 21 Aug 2008 13:23:12 -0400 10, 27 -- Subject: Re: [Microscopy] Negative stain of cyanobacteria 10, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 27 -- To: {vapatpxs-at-yahoo.com} , 10, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 27 -- Message-ID: {C4D31CC0.23A3%connellyps-at-nhlbi.nih.gov} 10, 27 -- Thread-Topic: [Microscopy] Negative stain of cyanobacteria 10, 27 -- Thread-Index: AckDspZz1P2Wl2+lEd2skgANk2Yv1A== 10, 27 -- In-Reply-To: {200808211528.m7LFSi5h018636-at-ns.microscopy.com} 10, 27 -- Mime-version: 1.0 10, 27 -- Content-type: text/plain; 10, 27 -- charset="ISO-8859-1" 10, 27 -- X-OriginalArrivalTime: 21 Aug 2008 17:23:36.0650 (UTC) FILETIME=[A5250AA0:01C903B2] 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7LHX67M031845 ==============================End of - Headers==============================
Dear Dorota, I have not seen an answer to your problem, so I will tell you what I do. For 200,000 times magnification, I would use Condenser aperture #2 and a spot size of 2 or 1 micron. Set those and then align. As you reduce your condenser aperture size and spot size, your beam will be more parallel, but less bright, so you have to balance resolution and contrast. Good luck,
-----Original Message----- X-from: Wadowska-at-upei.ca [mailto:Wadowska-at-upei.ca] Sent: August 21, 2008 6:03 AM To: maryflet-at-interchange.ubc.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College/UPEI
Title-Subject: [Filtered] high resulution mode Hitachi TEM
Question: Hello, I have a user who asked that her samples are scoped in HR mode at 200K. Is there a special aligment, beside a regular aligment done once in a while, of the scope that I have to do before I start scoping? Thanks Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both maryard-at-uga.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: maryard-at-uga.edu Name: Mary B. Ard
Organization: University of Georgia, Dept of Pathology
Title-Subject: [Filtered] Looking for book, Thin is in
Question: I am looking for the book, Thin is in: plastic embedding of tissue for light microscopy. The authors are Willard A. Burns and Ann Bretschneider. The copyright is 1981. I have either misplaced my copy somewhere, or I have lent it out to someone and it never returned. I am in need of the chapter that contains the special staining protocols.
The book is out of print, and I am having a difficult time finding another copy to purchase.
I have seen your problem and hope that I am able to help? A procedure is set out below that is taken from my web site hits and tips pages.
Imaging A number of controls are used during normal operation:-
(1) Focus (objective lens) controls, enable the beam to be adjusted to place the first image in the plain of the diffraction lens "in focus". This action should be carried out at double the photographic magnification to be used.
(2) Brightness or Second Condenser adjusts the intensity of the illumination on the specimen. Its alignment is corrected during operation with the Brightness or Illumination centring controls. Always reduce the illumination level by turning the control clockwise from focus. In this way the best beam coherence is achieved.
(3) Spot size, should be adjusted to attain a satisfactory image quality, in relation to the magnification, i.e. high resolution requires a spot size of {2µm normal operation 1-5µm.
(4) Objective Stigmators should be used like fine focus controls, to adjust the image sharpness, again use at double the photographic magnification.
(5) Objective Aperture is used to set the image contrast, it should be inserted and aligned in the diffraction mode.
(6) Magnification varies the strength of the imaging lenses which are automatically adjusted for minimum distortion.
(7) Illumination alignment should be corrected, to the screen centre, prior to focus at each new magnification
(8) Emission current should be adjusted to enable the illumination to be used in the over focus mode (high coherence) at the maximum magnification to be used. Insufficient emission will limit performance. Emission is controlled by the bias or by moving the filament forward (higher current) or backwards (lower current).
To emphasise a point, parallel beams are very important if you are chasing the best image quality, biology or materials, most times we need high coherence. There are misunderstandings on how to obtain a parallel beam or high coherence, setting the final condenser under focus is incorrect. The procedure for high coherence would be to use the smallest spot size you can tolerate (this probably means you must up the emission current). Once in this condition over focus the final condenser (clockwise from crossover) the spot, what ever size it is now, becomes your virtual source. The further over focus you go the greater the beam coherence and that is what we are after. You will deduce the smaller the condenser aperture the sharper the spot and the smaller the spot the greater the coherence for a given degree of over focus.
Work with a design team and they expect everyone to over focus, they expect everyone to use small condenser apertures and they expect everyone to use small spot sizes. They do not expect everyone to use too low an emission current (almost everyone does, we have talked before about filament life being the most important feature of many laboratories) because this makes the task too difficult!
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com -----Original Message----- X-from: Wadowska-at-upei.ca [mailto:Wadowska-at-upei.ca] Sent: 21 August 2008 13:56 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College/UPEI
Title-Subject: [Filtered] high resulution mode Hitachi TEM
Question: Hello, I have a user who asked that her samples are scoped in HR mode at 200K. Is there a special aligment, beside a regular aligment done once in a while, of the scope that I have to do before I start scoping? Thanks Dorota
Good luck finding a copy. Ann Bretshcneider works with me and even she no longer has a copy!
Geoff
maryard-at-uga.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both maryard-at-uga.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: maryard-at-uga.edu } Name: Mary B. Ard } } Organization: University of Georgia, Dept of Pathology } } Title-Subject: [Filtered] Looking for book, Thin is in } } Question: I am looking for the book, Thin is in: plastic embedding of } tissue for light microscopy. The authors are Willard A. Burns and } Ann Bretschneider. The copyright is 1981. I have either misplaced } my copy somewhere, or I have lent it out to someone and it never } returned. I am in need of the chapter that contains the special } staining protocols. } } The book is out of print, and I am having a difficult time finding } another copy to purchase. } } Thank you for any help in locating this book! } } Mary } } Login Host: 128.192.89.8 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Thu Aug 21 19:20:30 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7M0KS8P013622 } 9, 11 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2008 19:20:28 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c4d3b6bbdbf7-at-[206.69.208.22]} } 9, 11 -- Date: Thu, 21 Aug 2008 19:20:27 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: maryard-at-uga.edu (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Looking for book, Thin is in } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 28 -- From mcauliff-at-umdnj.edu Fri Aug 22 08:24:46 2008 8, 28 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7MDOki6014158 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 Aug 2008 08:24:46 -0500 8, 28 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 8, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 5647C4BE0C 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 Aug 2008 09:24:46 -0400 (EDT) 8, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 8, 28 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 93EEDA3B79 8, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 Aug 2008 09:24:45 -0400 (EDT) 8, 28 -- Received: from ([10.32.15.167]) 8, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.159341056; 8, 28 -- Fri, 22 Aug 2008 09:24:24 -0400 8, 28 -- MIME-version: 1.0 8, 28 -- Content-transfer-encoding: 7BIT 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 8, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 8, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0K6000A427WNMP40-at-umduwc01.umdnj.edu} for 8, 28 -- microscopy-at-microscopy.com; Fri, 22 Aug 2008 09:24:24 -0400 (EDT) 8, 28 -- Message-id: {48AEBE3C.2040100-at-umdnj.edu} 8, 28 -- Date: Fri, 22 Aug 2008 09:25:16 -0400 8, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 28 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 8, 28 -- To: maryard-at-uga.edu, microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] viaWWW: Looking for book, Thin is in 8, 28 -- References: {200808220022.m7M0M8XM015208-at-ns.microscopy.com} 8, 28 -- In-reply-to: {200808220022.m7M0M8XM015208-at-ns.microscopy.com} ==============================End of - Headers==============================
I experienced a similar situation with negatively staining some fimbriae-producing E.coli. The pili and fimbriae were negatively stained beautifully, but the bacterial cells themselves were crushed, just as you described for your cyanobacteria. I pretty sure that this collapse is due to air-drying, which is unavoidable when negative staining. Fixation in a (relatively) high concentration of gluteraldehyde *might* stabilize your cyanobacteria enough to be photogenic - I would try something like 3-6% glut - followed by conventional negative staining. You could also try positive staining followed by CPD or HMDS. The route I took was to CPD/HMDS and then platinum/carbon shadow. However, working with replicas is a lot more involved and tedious than a simple negative stain!
Andy Bowling USDA-ARS SWSRU
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Hello Listers,
I have a person who wants to negative stain marine cyanobacteria. Some are grown on agar plates and scraped off and others are grown in media. What we would like to know is...
What is the best way to negative stain these baceria? We have used 2% aq. UA and 1% PTA the stains work but not as well as we would like. We let the bugs sit for 5 minutes, wick off the excess and then have applied the stain from any where from 15 seconds to 1 minute.
We are definitely seeing the bacteria and they appear collapsed.
Any suggestions from the great collective mind of the list?
Thanks in advance,
Paula :-)
Paula Sicurello VA Medical Center San Diego Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 11, 24 -- From vapatpxs-at-yahoo.com Thu Aug 21 10:24:40 2008 11, 24 -- Received: from n77.bullet.mail.sp1.yahoo.com (n77.bullet.mail.sp1.yahoo.com [98.136.44.45]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7LFOd4u010866 11, 24 -- for {microscopy-at-microscopy.com} ; Thu, 21 Aug 2008 10:24:40 -0500 11, 24 -- Received: from [216.252.122.218] by n77.bullet.mail.sp1.yahoo.com with NNFMP; 21 Aug 2008 15:24:39 -0000 11, 24 -- Received: from [69.147.84.109] by t3.bullet.sp1.yahoo.com with NNFMP; 21 Aug 2008 15:24:39 -0000 11, 24 -- Received: from [127.0.0.1] by omp207.mail.sp1.yahoo.com with NNFMP; 21 Aug 2008 15:24:39 -0000 11, 24 -- X-Yahoo-Newman-Property: ymail-3 11, 24 -- X-Yahoo-Newman-Id: 647158.90977.bm-at-omp207.mail.sp1.yahoo.com 11, 24 -- Received: (qmail 56686 invoked by uid 60001); 21 Aug 2008 15:17:18 -0000 11, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 24 -- s=s1024; d=yahoo.com; 11, 24 -- h=Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-T ype:Message-ID; 11, 24 -- b=h6pviTv/nYyU/HD78g7UFppc9j/1WBVm3tmljDaKGQnaZptntaVznLzMtSOW7TLCi/Yx+o yvR4QMS8Yi3XaKYQuzR8bdvQhjRqOfzCMKQJdI9rJ1srBaQeET/W7YRxULhgrE4tpVHXN4Ub FDeXYdok7T98ROf4bYlroCtJbpDWc=; 11, 24 -- Received: from [24.30.157.32] by web46111.mail.sp1.yahoo.com via HTTP; Thu, 21 Aug 2008 08:17:18 PDT 11, 24 -- X-Mailer: YahooMailWebService/0.7.218 11, 24 -- Date: Thu, 21 Aug 2008 08:17:18 -0700 (PDT) 11, 24 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 11, 24 -- Reply-To: vapatpxs-at-yahoo.com 11, 24 -- Subject: Negative stain of cyanobacteria 11, 24 -- To: MSA BB {Microscopy-at-microscopy.com} 11, 24 -- MIME-Version: 1.0 11, 24 -- Content-Type: text/plain; charset=us-ascii 11, 24 -- Message-ID: {479465.43031.qm-at-web46111.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 17, 31 -- From Andrew.Bowling-at-ARS.USDA.GOV Fri Aug 22 09:27:38 2008 17, 31 -- Received: from messagescreen4.ars.usda.gov (messagescreen4.ars.usda.gov [199.133.180.151]) 17, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7MERbJv031696 17, 31 -- for {microscopy-at-microscopy.com} ; Fri, 22 Aug 2008 09:27:37 -0500 17, 31 -- Received: from CO-MAILBH-02.ARSNET.ARS.USDA.GOV ([199.133.183.227]) 17, 31 -- by messagescreen4.ars.usda.gov (8.13.8/8.13.8) with ESMTP id m7MEQdw3014087 17, 31 -- for {microscopy-at-microscopy.com} ; Fri, 22 Aug 2008 09:27:37 -0500 17, 31 -- Received: from CO-MAIL-03.ARSNET.ARS.USDA.GOV ([10.100.2.202]) by CO-MAILBH-02.ARSNET.ARS.USDA.GOV with Microsoft SMTPSVC(6.0.3790.3959); 17, 31 -- Fri, 22 Aug 2008 08:26:43 -0600 17, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 31 -- Content-class: urn:content-classes:message 17, 31 -- MIME-Version: 1.0 17, 31 -- Content-Type: text/plain; 17, 31 -- charset="US-ASCII" 17, 31 -- Subject: RE: [Microscopy] Negative stain of cyanobacteria 17, 31 -- Date: Fri, 22 Aug 2008 08:09:10 -0600 17, 31 -- Message-ID: {8017F94146BF634DA9414E4B9088525B025B6CE6-at-CO-MAIL-03.ARSNET.ARS.USDA.GOV} 17, 31 -- In-Reply-To: {200808211530.m7LFU8NU020580-at-ns.microscopy.com} 17, 31 -- X-MS-Has-Attach: 17, 31 -- X-MS-TNEF-Correlator: 17, 31 -- Thread-Topic: [Microscopy] Negative stain of cyanobacteria 17, 31 -- Thread-Index: AckDotyRFrEYVXBlRs2hgVocVSEEQgAGKFag 17, 31 -- From: "Bowling, Andrew" {Andrew.Bowling-at-ARS.USDA.GOV} 17, 31 -- To: {microscopy-at-microscopy.com} 17, 31 -- X-OriginalArrivalTime: 22 Aug 2008 14:26:43.0200 (UTC) FILETIME=[1972D800:01C90463] 17, 31 -- X-MessageScreenMessageID: 1219415257.749648.1254.167100439 17, 31 -- X-MessageScreenContentScore: Score of 0 assigned to Content 17, 31 -- X-MessageScreenUCEScore: Score of 0 assigned to UCE 17, 31 -- X-MessageScreen: Analyzed by IntelliReach MessageScreen(tm) 17, 31 -- Content-Transfer-Encoding: 8bit 17, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7MERbJv031696 ==============================End of - Headers==============================
I use a Philips CM30ST for HRTEM however the procedure should be quite similar for a Hitachi:
1/ standard alignement with a small spot and a small condenser (gun tilt, gun shift, condenser alignment, astigmatisme cond, specimen height, focus, pivot point, voltage modulation (rotcenter volt), 2/ coma free alignement (for the best resolution, not compulsory for standard HRTEM), 3/ choose a appropriate objective aperture, a larger aperture give higher resolution but less contrast. If you want to obtain lattice fringes images the aperture must be larger that the diffraction ring (or spot) you want to observe, 4/ center carrefully the objective aperture, 5/ check astimastism of the objective lens (use the fft device of your camera on a amorphous area), 6/ make an hysteris loop of the lenses (go from lower mag to higher mag and come back) 7/adjust the focus of the objective lens, slighly underfocus (scherzer defocus usually around -50nm). 8/ spread the illumination to have a parallel beam geometry. If there is a beam drift or a specimen drift if can useful to reduce the acquisition time by using a larger spot size, condenser or increase the illumination/emission.
Hope this can help
Patrick
----- Message d'origine ---- De : "Wadowska-at-upei.ca" {Wadowska-at-upei.ca} À : weis183-at-yahoo.fr Envoyé le : Jeudi, 21 Août 2008, 14h59mn 51s Objet : [Microscopy] viaWWW: high resulution mode Hitachi TEM
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Email: Wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College/UPEI
Title-Subject: [Filtered] high resulution mode Hitachi TEM
Question: Hello, I have a user who asked that her samples are scoped in HR mode at 200K. Is there a special aligment, beside a regular aligment done once in a while, of the scope that I have to do before I start scoping? Thanks Dorota
_____________________________________________________________________________ Envoyez avec Yahoo! Mail. Une boite mail plus intelligente http://mail.yahoo.fr
==============================Original Headers============================== 15, 21 -- From weis183-at-yahoo.fr Fri Aug 22 09:39:45 2008 15, 21 -- Received: from web26407.mail.ukl.yahoo.com (web26407.mail.ukl.yahoo.com [217.146.176.31]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7MEdiNl013206 15, 21 -- for {microscopy-at-microscopy.com} ; Fri, 22 Aug 2008 09:39:45 -0500 15, 21 -- Received: (qmail 9969 invoked by uid 60001); 22 Aug 2008 14:39:44 -0000 15, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 21 -- s=s1024; d=yahoo.fr; 15, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 15, 21 -- b=yyt452BqSUfXWbeKKv/ZkeJ1EDdxZOB7InrmSMcg9wZM7B+AYrh/+8YFA/0W2bx2EdTEBhhu3vDsaFmjoxz7n88g6fsvVdr5edbsMg53pv9bufViyokbGK47NS9pmvAD3oSVYdD4U1hezwYr17NwKtWUNlso9UyCplj15YWGVSc=; 15, 21 -- X-YMail-OSG: 3uQCZ2MVM1nzcC7QKlYpaLgY614JlIt3ynTFebJ1V54_4VjVa7wFXhnDi7rRb2e4SGmOD34u2ir1MzFeRmRtImt.ELCrztJAC6bdnvOfv0MAsdbl8FQgjRyFaR8uO1N10O_OVuxBbN3yFvMNFS16Ioi7 15, 21 -- Received: from [147.210.80.23] by web26407.mail.ukl.yahoo.com via HTTP; Fri, 22 Aug 2008 14:39:44 GMT 15, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218.2 15, 21 -- Date: Fri, 22 Aug 2008 14:39:44 +0000 (GMT) 15, 21 -- From: Patrick Weisbecker {weis183-at-yahoo.fr} 15, 21 -- Subject: Re : [Microscopy] viaWWW: high resulution mode Hitachi TEM 15, 21 -- To: microscopy-at-microscopy.com 15, 21 -- MIME-Version: 1.0 15, 21 -- Content-Type: text/plain; charset=iso-8859-1 15, 21 -- Message-ID: {606036.9630.qm-at-web26407.mail.ukl.yahoo.com} 15, 21 -- Content-Transfer-Encoding: 8bit 15, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7MEdiNl013206 ==============================End of - Headers==============================
George Mason University's Department of Atmospheric, Oceanic and Geological Sciences is looking to have petrological microscopes serviced. George Mason University is located at Fairfax, Virginia USA
This would be preventative maintenance - cleaning, lubrication, adjustments, alignments, and operational inspection for 18 microscopes.
10 Meiji ML 900 6 Olympus BHT 2 Olympus CX
We are looking for an estimate based on individual microscopes as well as a total package.
Please contact
Julia A Nord Cooper jnord-at-gmu.edu
==============================Original Headers============================== 6, 24 -- From gnord-at-mindspring.com Sat Aug 23 09:28:16 2008 6, 24 -- Received: from elasmtp-spurfowl.atl.sa.earthlink.net (elasmtp-spurfowl.atl.sa.earthlink.net [209.86.89.66]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7NESGdi016185 6, 24 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 09:28:16 -0500 6, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 24 -- s=dk20050327; d=mindspring.com; 6, 24 -- b=bwvbkH0zjxr1CcMfbX3V7zb+JEJuUca0e24zVqGrp4H2qUFGhyLdEtZzoarZM+hG; 6, 24 -- h=Received:Mime-Version:Content-Transfer-Encoding:Message-Id:Content-Type:To:From:Subject:Date:X-Mailer:X-ELNK-Trace:X-Originating-IP; 6, 24 -- Received: from [71.171.116.104] (helo=[192.168.254.6]) 6, 24 -- by elasmtp-spurfowl.atl.sa.earthlink.net with esmtpa (Exim 4.67) 6, 24 -- (envelope-from {gnord-at-mindspring.com} ) 6, 24 -- id 1KWu6B-0006Br-PN 6, 24 -- for microscopy-at-microscopy.com; Sat, 23 Aug 2008 10:28:15 -0400 6, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- Message-Id: {3C05D2DE-661D-434A-9097-0599C44BC099-at-mindspring.com} 6, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: Gordon Nord {gnord-at-mindspring.com} 6, 24 -- Subject: Petrographic Microscope Maintainance Needed 6, 24 -- Date: Sat, 23 Aug 2008 10:28:13 -0400 6, 24 -- X-Mailer: Apple Mail (2.753.1) 6, 24 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d888a63b7957ab9b23b3967dab46a01423c22e19a1cbf6021502350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 6, 24 -- X-Originating-IP: 71.171.116.104 ==============================End of - Headers==============================
I am looking for a color camera for light microscopes that is capable of both single images and also multiple exposures for making short movies. The camera does not have to be cooled but does have to be easy to use in a multi-user environment.
Budget is under $5000. Suggestions are appreciated from happy users. Vendors are encouraged to respond and demo or used cameras will be seriously considered.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 29 -- From dsherman-at-purdue.edu Sat Aug 23 12:10:17 2008 6, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7NHAGPk003078 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 12:10:16 -0500 6, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 6, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m7NH9tuQ029139 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 13:09:55 -0400 6, 29 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 6, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m7NH9sTI003869 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 13:09:55 -0400 6, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23] RDNS failed) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Sat, 23 Aug 2008 13:08:55 -0400 6, 29 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 6, 29 -- Sat, 23 Aug 2008 17:08:54 +0000 6, 29 -- User-Agent: Microsoft-Entourage/12.11.0.080522 6, 29 -- Date: Sat, 23 Aug 2008 13:08:53 -0400 6, 29 -- Subject: LM camera information 6, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 29 -- Message-ID: {C4D5BC65.20980%dsherman-at-purdue.edu} 6, 29 -- Thread-Topic: LM camera information 6, 29 -- Thread-Index: AckFQutGNIYtz09X7E+c5+r0lyu5bg== 6, 29 -- Mime-version: 1.0 6, 29 -- Content-type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-OriginalArrivalTime: 23 Aug 2008 17:08:55.0597 (UTC) FILETIME=[ECD285D0:01C90542] 6, 29 -- X-PMX-Version: 5.4.0.320885 6, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
A recent article in Microscopy Today showed a method for merging multiple images to form a color composite, using Photoshop. I discussed with John Russ his alternative method which offers an easy way to perform this alignment of the images. He has created a pdf file that can be downloaded from {www.drjohnruss.com/downloads/merge.pdf} " This illustrates with screen snapshots a method that anyone who is interested should download and compare.
John
-- John M. Mackenzie, Jr., PhD Professor of Microbiology Coordinator, Center for Electron Microscopy North Carolina State University Phone (919) 515-2664 Fax (919) 515-8293
==============================Original Headers============================== 5, 17 -- From john_mackenzie-at-ncsu.edu Sun Aug 24 17:14:31 2008 5, 17 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7OMEVE5005491 5, 17 -- for {Microscopy-at-microscopy.com} ; Sun, 24 Aug 2008 17:14:31 -0500 5, 17 -- Received: from [127.0.0.1] (really [66.57.53.232]) 5, 17 -- by cdptpa-omta04.mail.rr.com with ESMTP 5, 17 -- id {20080824221431.PCCO13299.cdptpa-omta04.mail.rr.com-at-[127.0.0.1]} ; 5, 17 -- Sun, 24 Aug 2008 22:14:31 +0000 5, 17 -- Message-ID: {48B1DCC1.1010902-at-ncsu.edu} 5, 17 -- Date: Sun, 24 Aug 2008 18:12:17 -0400 5, 17 -- From: John Mackenzie {john_mackenzie-at-ncsu.edu} 5, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 5, 17 -- MIME-Version: 1.0 5, 17 -- To: Microscopy-at-microscopy.com 5, 17 -- Subject: Re: Image Merging: An alternative method 5, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
----- Original Message ----- X-from: {spamMfilter-at-microscopy.com} To: {hubner-at-iod.krakow.pl} Sent: Monday, August 25, 2008 10:13 AM
Hello. I have a question about our Olympus SIS Veleta camera: It shows a noisy background over certain ranges of exposure times. The problem is especially obvious in dark-field images and diffraction patterns, where we use long exposure times and expect a dark background.
X-from about 3-200 ms exposure time, we see a noisy horizontal band across the image. The position of the band changes with exposure time. Beyond about 500 ms, bright spots start to appear, actually short vertical streaks several pixels long, in a fixed arrangement. The number and brightness of these blemishes increase with exposure time. Then beyond 4-s exposure time, a whole series of horizontal bands start to appear. Surprisingly, the blemishes don't appear on every frame; maybe 1 in 20 shows no blemishes, which is also puzzling. I put some examples here: http://ahrenkiel.sdsmt.edu/H7000_TEM/Veleta.
To correct this within iTEM, the dark reference images have to be acquired for the specific exposure time used. (I learned that the reference exposure settings can be changed by holding "shift" while selecting "Acquire Reference Images"). Unfortunately, all the input channels for a particular device seem to use the same reference images, so we can't just make a new channel for a different type of acquisition.
Does anyone know where this noise is coming from? My guess is this some kind of read-out noise, but the OSIS literature for the Veleta indicates that noise is reduced via innovative read-out technology.
A median filter helps to remove the bad pixels, but that throws away a significant fraction of the data. I also wrote an Imaging-C program that sums several images. As long as the individual exposures are properly corrected, the noise is avoided. Using either method, the noise still appears during acquisition, prior to taking a "snapshot", which is annoying.
Is anyone else doing Imaging-C programming for TEM? If so, I would be very much like to communicate with you, maybe share our code. This seems like my only hope to solve this type of problem. Thanks. ------------------------------------------ Phil Ahrenkiel, Assistant Professor Nanoscience and Nanoengineering Ph.D. Program South Dakota School of Mines and Technology 501 E. Saint Joseph St. Rapid City, South Dakota 57701 Office: EP 221 Phone: 605-394-5238, Fax: 605-394-2365 Email: Phil.Ahrenkiel-at-sdsmt.edu
==============================Original Headers============================== 9, 37 -- From Phil.Ahrenkiel-at-sdsmt.edu Mon Aug 25 10:43:51 2008 9, 37 -- Received: from silver.sdsmt.edu (silver.sdsmt.edu [151.159.1.1]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7PFhoeh008886 9, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 10:43:51 -0500 9, 37 -- Received: from owa.sdsmt.edu (owa.sdsmt.edu [151.159.3.7]) 9, 37 -- by silver.sdsmt.edu (8.14.2/8.14.2) with ESMTP id m7PFhn72015480 9, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 09:43:49 -0600 9, 37 -- DomainKey-Signature: a=rsa-sha1; s=sdsmtdk; d=sdsmt.edu; c=nofws; q=dns; 9, 37 -- h=dkim-signature:x-mimeole:content-class:mime-version: 9, 37 -- content-type:content-transfer-encoding:subject:date:message-id: 9, 37 -- x-ms-has-attach:x-ms-tnef-correlator:thread-topic:thread-index:from:to; 9, 37 -- b=Nhh3EkcpLTa1GMKC/aGukuP9MSHaw564UZM1rMPm1rcVL3fSXHez60QUdBPWsdmei 9, 37 -- XWvr70OBh0xLVoH6tc02qhvLRA8zeSCnl42fnaoONX2WZIY7Q4xkhSA20QJs3SWS/p/ 9, 37 -- sqh5+fyiyj8k93aaxhX/ZPsOObcZuC+V0YYX62E= 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sdsmt.edu; 9, 37 -- s=sdsmtdkim; t=1219679029; bh=gB38h0v/yWTFzjSTnRs+bzJ7izyprxI4Snfs+ 9, 37 -- kZPwok=; h=MIME-Version:Content-Type:Content-Transfer-Encoding: 9, 37 -- Subject:Date:Message-ID:From:To; b=ZLMkG9z476N93vIdwHnQTaAfhRnR8eA 9, 37 -- My40F5Khjbp6KgCszuk5UjgdOn7bDMt5zSfZdIC3WFlO4ZqwP6RZXyTK+S1EqNAttHB 9, 37 -- Dq//FpEx98z7tsHEPWfl/XoeOHIJfHRVnOcwR34ug2BQEOWkPSQbCWm35jJQ7jxmcsb 9, 37 -- ylhSA8= 9, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 37 -- Content-class: urn:content-classes:message 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; 9, 37 -- charset="us-ascii" 9, 37 -- Subject: Veleta camera issues 9, 37 -- Date: Mon, 25 Aug 2008 09:43:42 -0600 9, 37 -- Message-ID: {6FD01225FACD9C4AA3870724330536F40A21DBCA-at-sdsmt-ex01.SDSMT.LOCAL} 9, 37 -- X-MS-Has-Attach: 9, 37 -- X-MS-TNEF-Correlator: 9, 37 -- Thread-Topic: Veleta camera issues 9, 37 -- Thread-Index: AckGyVm3kEpwO5nTSSa0/cEZ7CKZ7A== 9, 37 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu} 9, 37 -- To: {Microscopy-at-microscopy.com} 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7PFhoeh008886 ==============================End of - Headers==============================
Dear Debby, I have had good results from the Motic camera (www.motic.com). I bought the cheapest model, about $250, but it came with full software and every adapter needed to attach to any microscope and included the fast USB card, in case your computer didn't have one. Even had a stage micrometer for calibration included. The software includes auto multiple exposures. Regards,
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: August 23, 2008 10:18 AM To: maryflet-at-interchange.ubc.ca
I am looking for a color camera for light microscopes that is capable of both single images and also multiple exposures for making short movies. The camera does not have to be cooled but does have to be easy to use in a multi-user environment.
Budget is under $5000. Suggestions are appreciated from happy users. Vendors are encouraged to respond and demo or used cameras will be seriously considered.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 29 -- From dsherman-at-purdue.edu Sat Aug 23 12:10:17 2008 6, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7NHAGPk003078 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 12:10:16 -0500 6, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 6, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m7NH9tuQ029139 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 13:09:55 -0400 6, 29 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 6, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m7NH9sTI003869 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 23 Aug 2008 13:09:55 -0400 6, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23] RDNS failed) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Sat, 23 Aug 2008 13:08:55 -0400 6, 29 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 6, 29 -- Sat, 23 Aug 2008 17:08:54 +0000 6, 29 -- User-Agent: Microsoft-Entourage/12.11.0.080522 6, 29 -- Date: Sat, 23 Aug 2008 13:08:53 -0400 6, 29 -- Subject: LM camera information 6, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 29 -- Message-ID: {C4D5BC65.20980%dsherman-at-purdue.edu} 6, 29 -- Thread-Topic: LM camera information 6, 29 -- Thread-Index: AckFQutGNIYtz09X7E+c5+r0lyu5bg== 6, 29 -- Mime-version: 1.0 6, 29 -- Content-type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-OriginalArrivalTime: 23 Aug 2008 17:08:55.0597 (UTC) FILETIME=[ECD285D0:01C90542] 6, 29 -- X-PMX-Version: 5.4.0.320885 6, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
==============================Original Headers============================== 15, 32 -- From maryflet-at-interchange.ubc.ca Mon Aug 25 10:54:14 2008 15, 32 -- Received: from mr7.mail-relay.ubc.ca (mr7.mail-relay.ubc.ca [137.82.45.13]) 15, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7PFsEB6022218 15, 32 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 10:54:14 -0500 15, 32 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 15, 32 -- by mr7.mail-relay.ubc.ca (Postfix) with ESMTP id 81F7F1C210 15, 32 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 08:54:13 -0700 (PDT) 15, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 15, 32 -- by smtp.interchange.ubc.ca 15, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 15, 32 -- with ESMTP id {0K6500HZ9YUCQQ-at-smtp.interchange.ubc.ca} for 15, 32 -- microscopy-at-microscopy.com; Mon, 25 Aug 2008 08:54:13 -0700 (PDT) 15, 32 -- Date: Mon, 25 Aug 2008 08:53:41 -0700 15, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 15, 32 -- Subject: RE: [Microscopy] LM camera information 15, 32 -- In-reply-to: {200808231717.m7NHHq34013018-at-ns.microscopy.com} 15, 32 -- To: dsherman-at-purdue.edu 15, 32 -- Cc: microscopy-at-microscopy.com 15, 32 -- Reply-to: maryflet-at-interchange.ubc.ca 15, 32 -- Message-id: {0K6500HZAYUCQQ-at-smtp.interchange.ubc.ca} 15, 32 -- Organization: Materials Eng. 15, 32 -- MIME-version: 1.0 15, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 15, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 15, 32 -- Content-type: text/plain; charset=US-ASCII 15, 32 -- Content-transfer-encoding: 7bit 15, 32 -- Thread-index: AckFRC5UGw7h2m+nR4uwWkfAyAjpugBhfg9A 15, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.8.25.153406 15, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 15, 32 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, ECARD_WORD 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_MONEY 0, __FRAUD_419_MONEY_VALUE 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 15, 32 -- X-Spam-Level: 15, 32 -- X-Spam-Flag: No ==============================End of - Headers==============================
I wonder if anyone knows a source to get a copy of Kirkland's Book "Advanced Computing in Electron Microscopy". Currently the book is out of print.
Thank You,
Jafar
Jafar F. Al-Sharab
Rutgers, The State University of New Jersey Department of Materials Science and Engineering McLaren Center Ceramics Research 607 Taylor Road, Room 237 Piscataway, NJ 08854-8065 Tel. 732-445-5615 Fax 732-445-3258
==============================Original Headers============================== 10, 25 -- From jafarhan-at-rci.rutgers.edu Mon Aug 25 22:10:42 2008 10, 25 -- Received: from annwn13.rutgers.edu (smtp.rutgers.edu [128.6.72.243]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7Q3AfKo001262 10, 25 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 22:10:41 -0500 10, 25 -- Received: from localhost (localhost.rutgers.edu [127.0.0.1]) 10, 25 -- by annwn13.rutgers.edu (Postfix) with ESMTP id 81EF0324042 10, 25 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 23:10:41 -0400 (EDT) 10, 25 -- Received: from annwn13.rutgers.edu ([127.0.0.1]) 10, 25 -- by localhost (annwn13.rutgers.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id 30807-01 for {microscopy-at-microscopy.com} ; 10, 25 -- Mon, 25 Aug 2008 23:10:41 -0400 (EDT) 10, 25 -- Received: from galt.rci.rutgers.edu (pool-68-239-220-185.nwrk.east.verizon.net [68.239.220.185]) 10, 25 -- by annwn13.rutgers.edu (Postfix) with ESMTP id 3F5D232403F 10, 25 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 23:10:41 -0400 (EDT) 10, 25 -- Message-Id: {7.0.1.0.2.20080825225529.025aedc8-at-rci.rutgers.edu} 10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 25 -- Date: Mon, 25 Aug 2008 23:06:09 -0400 10, 25 -- To: microscopy-at-microscopy.com 10, 25 -- From: Jafar Al-Sharab {jafarhan-at-rci.rutgers.edu} 10, 25 -- Subject: Kirkland's book " Advanced Computing in Electron Microscopy" 10, 25 -- In-Reply-To: {200808251555.m7PFtvXD025616-at-ns.microscopy.com} 10, 25 -- References: {200808251555.m7PFtvXD025616-at-ns.microscopy.com} 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 25 -- X-Virus-Scanned: Virus Scanned by NBCS ==============================End of - Headers==============================
I wonder if anyone knows a source to get a copy of Kirkland's Book "Advanced Computing in Electron Microscopy". Currently the book is out of print and only digital format "kindal" are available from Amazon.
Thank You,
Jafar
Jafar F. Al-Sharab
Rutgers, The State University of New Jersey Department of Materials Science and Engineering McLaren Center Ceramics Research 607 Taylor Road, Room 237 Piscataway, NJ 08854-8065 Tel. 732-445-5615 Fax 732-445-3258
==============================Original Headers============================== 10, 24 -- From jafarhan-at-rci.rutgers.edu Mon Aug 25 22:10:42 2008 10, 24 -- Received: from annwn13.rutgers.edu (smtp.rutgers.edu [128.6.72.243]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7Q3AfDr001264 10, 24 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 22:10:42 -0500 10, 24 -- Received: from localhost (localhost.rutgers.edu [127.0.0.1]) 10, 24 -- by annwn13.rutgers.edu (Postfix) with ESMTP id D1F9332404B 10, 24 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 23:10:41 -0400 (EDT) 10, 24 -- Received: from annwn13.rutgers.edu ([127.0.0.1]) 10, 24 -- by localhost (annwn13.rutgers.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 24 -- with ESMTP id 30576-06 for {microscopy-at-microscopy.com} ; 10, 24 -- Mon, 25 Aug 2008 23:10:41 -0400 (EDT) 10, 24 -- Received: from galt.rci.rutgers.edu (pool-68-239-220-185.nwrk.east.verizon.net [68.239.220.185]) 10, 24 -- by annwn13.rutgers.edu (Postfix) with ESMTP id 9A2A532403F 10, 24 -- for {microscopy-at-microscopy.com} ; Mon, 25 Aug 2008 23:10:41 -0400 (EDT) 10, 24 -- Message-Id: {7.0.1.0.2.20080825230803.025a71d8-at-rci.rutgers.edu} 10, 24 -- Message-Id: {7.0.1.0.2.20080825225529.025aedc8-at-rci.rutgers.edu} 10, 24 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 10, 24 -- Date: Mon, 25 Aug 2008 23:10:45 -0400 10, 24 -- To: microscopy-at-microscopy.com 10, 24 -- From: Jafar Al-Sharab {jafarhan-at-rci.rutgers.edu} 10, 24 -- Subject: Kirkland's book " Advanced Computing in Electron Microscopy" 10, 24 -- Mime-Version: 1.0 10, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 24 -- X-Virus-Scanned: Virus Scanned by NBCS ==============================End of - Headers==============================
I have a copy I can part with, if you haven't gotten one already. I'll put it in the mail today or tomorrow (TEM class starts today.
Phil
} Dear Listers, } } I wonder if anyone knows a source to get a copy of Kirkland's Book } "Advanced Computing in Electron Microscopy". Currently the book is } out of print and only digital format "kindal" are available from Amazon. } } Thank You, } } Jafar } } } } Jafar F. Al-Sharab } } Rutgers, The State University of New Jersey } Department of Materials Science and Engineering } McLaren Center Ceramics Research } 607 Taylor Road, Room 237 } Piscataway, NJ 08854-8065 } Tel. 732-445-5615 } Fax 732-445-3258 -- Philip Oshel Technical Editor, Microscopy Today Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 26 -- From oshel1pe-at-cmich.edu Tue Aug 26 07:10:40 2008 4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7QCAeRm022444 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 07:10:40 -0500 4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m7QCAcS0007036 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 08:10:39 -0400 4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 26 -- Tue, 26 Aug 2008 08:09:44 -0400 4, 26 -- Mime-Version: 1.0 4, 26 -- Message-Id: {f06240800c4d9a2b89362-at-[141.209.160.249]} 4, 26 -- In-Reply-To: {200808260316.m7Q3GTOp013459-at-ns.microscopy.com} 4, 26 -- References: {200808260316.m7Q3GTOp013459-at-ns.microscopy.com} 4, 26 -- Date: Tue, 26 Aug 2008 08:09:42 -0400 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 26 -- Subject: Re: [Microscopy] Kirkland's book " Advanced Computing in Electron 4, 26 -- Microscopy" 4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 26 -- X-OriginalArrivalTime: 26 Aug 2008 12:09:44.0442 (UTC) FILETIME=[A056E5A0:01C90774] 4, 26 -- X-Canit-CHI2: 0.00 4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 26 -- X-CanItPRO-Stream: default 4, 26 -- X-Canit-Stats-ID: 1666272 - a99053786c4b 4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dawgdeluxe-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dawgdeluxe-at-gmail.com Name: Farid Jalali
Organization: STTARR Innovation Facility/ Toronto Medical Discovery Towers
Title-Subject: [Filtered] Vacuolar DAPI staining in xenograft sections
Question: Hello Group, I have a colleague who is getting very poor nuclear morphology from tumour xenografts that are fixed in 10% neutral buffered formalin for 24 hours, and then transferred to 70% ethanol. They are subsequently paraffin embedded and sectioned to 4um. The H and E stains also reflect poor nuclear morphology but it is most clearly evident when the sections are stained with 0.1ug/ml DAPI and mounted with Vectashield for widefield or confocal imaging. We are accustomed to seeing nuclei that are generally homogeneously stained, with a couple of nucleoli that are well defined. The problematic sections seem more vacuolar in appearance. These tumours do not have as many nucleoli as the image may suggest. Also, when stained with a marker that forms punctate foci within nuclei, the marker seems to accumulate on the edges of the more dense DAPI staining, rather than be distributed through the nucleus.
Any thoughts on this sample prep question would be greatly appreciated. I have images but have not figured out how to post them with the message.
Hello all! Before I start presenting my problem, I must say that I was in no way involved in the preparation of the material, I was not even given the occasion to offer some advices. It is important to say it because the way the material was treated is terrible in my point of view. Several rat organs where dissected and immediately fixed at 4°C in (I think) 7,5% formalin. They were probably left at 4°C in formalin for approx. 3 monthes. Then the solution was replaced with isopropanol and the material was left at -80°C. It is most probably isopropanol (I am not sure) because the liquid is not completely frozen at -80°C, just pretty thick. Now I have been asked to make miracles, which means to embed these organs and to give decent pictures in TEM (or al least to try my best). I planned to make the following and wanted to ask you not about what has been done until now, but what would be best from now on. - Place the samples on crushed ice until the t° reaches 0-4°C - Replace Isopropanol with glutaraldehyde and leave overnight at 4°C. - Dehydrate in ethanol and embed in Epon very classically. I really wonder if it makes much sense to fix again in glutaraldehyde, but I just thought that it could not hurt. Regards, Stephane
==============================Original Headers============================== 4, 21 -- From nizets2-at-yahoo.com Tue Aug 26 10:00:46 2008 4, 21 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7QF0jsx025999 4, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 10:00:45 -0500 4, 21 -- Received: (qmail 77699 invoked by uid 60001); 26 Aug 2008 15:00:45 -0000 4, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 21 -- s=s1024; d=yahoo.com; 4, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 4, 21 -- b=qmQD34olU/mppzHvQ27mlHaiGUaANSPN9vaa6liGTX4Cb2IPqXPPWN1IuAvX6TZPlqEOMTuuzHSPl0+vcfgZyiLvt2/lu4WCCWqo0saY97h2wDLEhvfkGccBZg35gD9NX7ZYjoUQMb1EDAR2eRARXZQ19hhUILoDXe4GuKWZGdk=; 4, 21 -- X-YMail-OSG: c8xuZZMVM1lw9YNXb.iMXojYlN3E6GTfGmLXpHTyjRSJ5X97Jp6UNYq2qzj1uOC.0Jmkj46434jvdrJ7Vr.WjJtrJhpoUp0w.ez8GAGQ5W6pu3CZwLfZfTawy6Hb4JoO1Sw- 4, 21 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Tue, 26 Aug 2008 08:00:45 PDT 4, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218.2 4, 21 -- Date: Tue, 26 Aug 2008 08:00:45 -0700 (PDT) 4, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 4, 21 -- Subject: Problem of thawing frozen organs 4, 21 -- To: microscopy-at-microscopy.com 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; charset=iso-8859-1 4, 21 -- Message-ID: {220759.77663.qm-at-web37406.mail.mud.yahoo.com} 4, 21 -- Content-Transfer-Encoding: 8bit 4, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7QF0jsx025999 ==============================End of - Headers==============================
Here is the September 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Thursday August 28, 2008. Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor
===========================
A Scanning Tunneling Microscope as a Switch in a Nanocompter Stephen W. Carmichael, Mayo Clinic
Atom-Probe Microscopy LEAPs the Chasm to Mainstream Applications Roger Alvis* and Thomas F. Kelly**, *FEI Company and **Imago Scientific Instruments
Freezing Techniques: History, Comparisons, and Applications B. Graham, Bibst Labs, Brookline, NH, J. R. Austin II, University of Chicago Chicago, IL, A. Kaech, Univ. of Zurich, Switzerland, J. E. Heuser, Washington Univ. School of Med., St. Louis, MO
Using EBSD to Map Domain Structures in Ferroelectrics I. MacLaren, M. U. Farooq, R. Villaurrutia, Dept. of Physics and Astronomy, University of Glasgow, UK
Multicolor Contrast Effects by Monochromatic Astronomic Filters–Utilization in Light Microscopy and Photomicrography JÖrg Piper, Clinic Meduna, Bad Bertrich, Germany
LED Light Source: Major Advance in Fluorescence Microscopy B. Hohman, Carl Zeiss MicroImaging, Inc., Thornwood, NY
Microscopy and Microanalysis of Magmatic and Metamorphic Minerals – Part 1: Cordierite Robert Sturm, Salzburg, Austria
CellProfiler: Open-Source Software to Automatically Quantify Images Martha S. Vokes and Anne E. Carpenter, The Broad Institute of MIT and Harvard, Cambridge, MA
Nanotectonica: Architectural Design Studio and Table Top SEM Jonas Coersmeier* and Donovan N. Leonard**, *Pratt Institute, Brooklyn, NY, **Appalachian State University, Boone, NC
Online Microscopy Lab Training Modules in an Academic Environment K. Schierbeek,* A. Mikel,** S. E. Hill,* and O. P. Mills*, *Mich. Tech. Univ., Houghton, MI, **Cummins Tech. Ctr, Columbus IN
Designing a Rate System for an Imaging Recharge Center Debby Sherman, Life Science Microscopy Facility, Purdue Univ., West Lafayette, IN
The Open_Cut Project - An Interest Group Concerned With Older Ultramicrotomes That No Longer Have Original Vendor Support Dale A. Callaham, The Univ. of Massachusetts, Amherst, MA
Determining the Relationship Between the Diameter of an Objective Aperture and Its Subtended Angle Hendrik O. Colijn, Ohio State Univ. Columbus, OH
Industry News
NetNotes SPECIMEN PREPARATION – propylene oxide SPECIMEN PREPARATION - Epon failing to polymerize SPECIMEN PREPARATION – LR White polymerization SPECIMEN PREPARATION - tissue culture embedding SPECIMEN PREPARATION – Staining for suberin and lignin SPECIMEN PREPARATION - stain artifact SPECIMEN PREPARATION - black precipitate in sperm TEM sections SPECIMEN PREPARATION - TEM stabilization layer for delicate polymers SPECIMEN PREPARATION – SEM of small round samples with charging SPECIMEN PREPARATION – sputter coating atmosphere SPECIMEN PREPARATION - small, irreplaceable mineral grains for analysis SPECIMEN PREPARATION - making support films for slotted grids MICROTOMY - cleaning a diamond knife IMMUNOCYTOCHEMISTRY – re-using antibodies
Dear Abbe
Advertiser's Index
==============================Original Headers============================== 22, 17 -- From randerson20-at-tampabay.rr.com Tue Aug 26 15:55:46 2008 22, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123]) 22, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7QKtk6e024485 22, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 Aug 2008 15:55:46 -0500 22, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 22, 17 -- by hrndva-omta06.mail.rr.com with ESMTP 22, 17 -- id {20080826205545.FAQC2273.hrndva-omta06.mail.rr.com-at-[127.0.0.1]} 22, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 26 Aug 2008 20:55:45 +0000 22, 17 -- Message-ID: {48B46DCC.80703-at-tampabay.rr.com} 22, 17 -- Date: Tue, 26 Aug 2008 16:55:40 -0400 22, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 22, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 22, 17 -- MIME-Version: 1.0 22, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 22, 17 -- Subject: September 2008 Microscopy Today Table of Contents 22, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 22, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
My suggestion would be to do two preps in parallel.
One would be: transfer to acetone while keeping at -80° C, then do freeze substitution (try the TA-UrAc Freeze sub I use, if you like; 0.2% TA in acetone at -80° overnight, warm to 0°, rinse out the TA and do UrAc in acetone (0.2-2%, matters little) for 1-2 hr, then infiltrate & embed in epoxy. That should show you the ice-crustal segregation "ghosts" and consequent deformations of cell and tissue structure.
The other would be: to warm to 0° in isopropanol, then give it a few hours to reabsorb the melting ice crystals and for the cell & tissue structure to "heal" the segregation areas by elastic recovery. I think this might work better if you transfer to an aqueous physiological buffer first, as the isopropanol may restrict or modify the "healing" process. THEN expose to glutaraldehyde in the same "healing" buffer or isopropanol. I've read or heard about such "healing" somewhere as being surprisingly complete after rewarming carelessly frozen tissues (i.e., you can't detect the locations or effects of the ice crystals !?); I imagine it would be helped by the prior formalin exposure having hardened the "native" structure before the propanol. Probably good not to delay more than a couple of hours during the "healing-reannealing" stage before glutaraldehyde, because formaldehyde fix is slowly reversible by water washing-- hours to days I think. (100% Formalin = saturated water solution (40%) of formaldehyde I think).
good luck!
-mike reedy-
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-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
Is there enough material to split in 2-3 portions? :) Your plan sounds good, assuming, from your description, that the material is far from being wholly dehydrated and *might* have been cryoprotected, so perhaps rehydration and GA fix before proper dehydration will indeed bring some improvement?.. I am not really sure, haven't done that. Stronger aldehyde fixation helps against dehydration shock, sounds like it may be too late for that... Essentially, I understand you've got weakly fixed (7.5% formalin = 2.8% formaldehyde?) tissue put directly into isopropanol, then into -80C. Interesting. Not necessarily disastrous, although you saying "organs" indicates large pieces, in which case the tissue inside might have gotten ice crystals...
After, rehydration and GA treatment, will you do OsO4 as well? you probably should, why not?
This does remind me of something I have done with small, EM size, pieces a few times, without any ill effects - I used to leave the pieces, after the usual GA-Os-ethanols, in 70% or 100% ethanol in a 20degC freezer. For 2-3 weeks. Result was just as good as usual (i.e., good :) ). This is to say, I would take a portion and proceed right away with proper dehydration, e.g., by introducing gradually acetone.
Good luck, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello all! } Before I start presenting my problem, I must say that I was in no } way involved in the preparation of the material, I was not even } given the occasion to offer some advices. It is important to say it } because the way the material was treated is terrible in my point of } view. } Several rat organs where dissected and immediately fixed at 4°C in } (I think) 7,5% formalin. They were probably left at 4°C in formalin } for approx. 3 monthes. } Then the solution was replaced with isopropanol and the material } was left at -80°C. It is most probably isopropanol (I am not sure) } because the liquid is not completely frozen at -80°C, just pretty } thick. } Now I have been asked to make miracles, which means to embed these } organs and to give decent pictures in TEM (or al least to try my } best). } I planned to make the following and wanted to ask you not about } what has been done until now, but what would be best from now on. } - Place the samples on crushed ice until the t° reaches 0-4°C } - Replace Isopropanol with glutaraldehyde and leave overnight at 4°C. } - Dehydrate in ethanol and embed in Epon very classically. } I really wonder if it makes much sense to fix again in } glutaraldehyde, but I just thought that it could not hurt. } Regards, } Stephane } } } } } } ==============================Original } Headers============================== } 4, 21 -- From nizets2-at-yahoo.com Tue Aug 26 10:00:46 2008 } 4, 21 -- Received: from web37406.mail.mud.yahoo.com } (web37406.mail.mud.yahoo.com [209.191.91.138]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m7QF0jsx025999 } 4, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 } 10:00:45 -0500 } 4, 21 -- Received: (qmail 77699 invoked by uid 60001); 26 Aug 2008 } 15:00:45 -0000 } 4, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 4, 21 -- s=s1024; d=yahoo.com; } 4, 21 -- h=X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content- } Transfer-Encoding:Message-ID; } 4, 21 -- b=qmQD34olU/ } mppzHvQ27mlHaiGUaANSPN9vaa6liGTX4Cb2IPqXPPWN1IuAvX6TZPlqEOMTuuzHSPl0 } +vcfgZyiLvt2/ } lu4WCCWqo0saY97h2wDLEhvfkGccBZg35gD9NX7ZYjoUQMb1EDAR2eRARXZQ19hhUILoDX } e4GuKWZGdk=; } 4, 21 -- X-YMail-OSG: } c8xuZZMVM1lw9YNXb.iMXojYlN3E6GTfGmLXpHTyjRSJ5X97Jp6UNYq2qzj1uOC. } 0Jmkj46434jvdrJ7Vr.WjJtrJhpoUp0w.ez8GAGQ5W6pu3CZwLfZfTawy6Hb4JoO1Sw- } 4, 21 -- Received: from [80.122.101.100] by } web37406.mail.mud.yahoo.com via HTTP; Tue, 26 Aug 2008 08:00:45 PDT } 4, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218.2 } 4, 21 -- Date: Tue, 26 Aug 2008 08:00:45 -0700 (PDT) } 4, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 4, 21 -- Subject: Problem of thawing frozen organs } 4, 21 -- To: microscopy-at-microscopy.com } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- Content-Type: text/plain; charset=iso-8859-1 } 4, 21 -- Message-ID: {220759.77663.qm-at-web37406.mail.mud.yahoo.com} } 4, 21 -- Content-Transfer-Encoding: 8bit } 4, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m7QF0jsx025999 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 24 -- From vladislav_speransky-at-nih.gov Tue Aug 26 16:30:20 2008 8, 24 -- Received: from nihrelayxway2.hub.nih.gov (nihrelayxway2.hub.nih.gov [128.231.90.107]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7QLUIuv019103 8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 16:30:19 -0500 8, 24 -- X-IronPortListener: NIH_Relay 8, 24 -- X-SBRS: None 8, 24 -- X-IronPort-AV: E=Sophos;i="4.32,273,1217822400"; 8, 24 -- d="scan'208";a="35008285" 8, 24 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 8, 24 -- by nihrelayxway2.hub.nih.gov with ESMTP; 26 Aug 2008 17:30:17 -0400 8, 24 -- Received: from [128.231.217.179] (db4179.niaid.nih.gov [128.231.217.179]) 8, 24 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id m7QLUGQ0013582 8, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 17:30:17 -0400 8, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) 8, 24 -- References: {200808261502.m7QF22qx027412-at-ns.microscopy.com} 8, 24 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 8, 24 -- Message-Id: {1A822EC9-4D9E-4175-BE54-B4FED716B95E-at-nih.gov} 8, 24 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 24 -- Subject: Fwd: [Microscopy] Problem of thawing frozen organs 8, 24 -- Date: Tue, 26 Aug 2008 17:30:15 -0400 8, 24 -- To: Microscopy-at-microscopy.com 8, 24 -- X-Mailer: Apple Mail (2.753.1) 8, 24 -- Content-Transfer-Encoding: 8bit 8, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7QLUIuv019103 ==============================End of - Headers==============================
We are having some trouble in our lab getting immunogold labeling on epitope tagged materials to work. Here's the background:
We have a number of different projects on the go, all of which we would like to use epitope tagged proteins for immunogold labeling. We have 3 different proteins we've labelled, 2 of which have YFP, one of which has a Myc tag, and another (which we have not tried yet in TEM) which has a His-tag. In each case the label can be localized using other methods. In the case of the YFP-tag, the label can be seen using live cell imaging, and in the case of the Myc and His-tags, Western blots confirm the presence of the tagged protein.
Our problem is getting the protein to label sections prepared for TEM. Our standard procedure for immunolabelling is to high pressure freeze, freeze substitute in 0.25% glut, 2% UA in acetone, and then embed in LR White at room temperature. In the case of the YFP label we could see label, but there seems to be no consistency or pattern that we could discern, especially compared to our live cell results. In the case of the Myc tag, we can get no label whatsoever, using a few different anti-Myc labels.
Because we were having trouble with the Myc label, we decided to try several different preservation techniques. We tried low-temperature embedding in lowicryl HM20 or K4M, and in both cases the waxy cuticle of our arabidopsis stems (and seeds) resulted in the samples simply falling out of the blocks. Our only explanation is that the waxes were so well preserved by these methods that they didn't allow for proper preservation. According to others, this is reminiscent of the problem that drosophila experts have when embedding embryos that have developed a cuticle.
We have also tried etching both our LR white samples, and osmicated samples (embedded in Spurr's) prior to labelling, with no success.
My question to you is whether anyone has a tried and true method for embedding difficult samples for for immunolabelling? We're desperate at this point, and willing to try almost anything. Our samples (Arabidopsis stems and developing seeds) have waxy cuticles that are both important to our analysis and part of the reason we have so much trouble embedding our material. We have used a number of different epitope tags and antibodies, with virtually no success, which is why we are currently trying to optimize our embedding techniques, rather than trying different epitopes tags.
Thanks in advance for any insight that you might have. I'm sorry that this is so long. Cheers, Robin
Robin Young, M.Sc. PhD Candidate, Samuels and Haughn Labs Botany Dept, University of British Columbia 6270 University Blvd, Vancouver, BC V6K 1E4 / Fax: 604.822.6089
==============================Original Headers============================== 12, 26 -- From youngre-at-interchange.ubc.ca Tue Aug 26 16:49:02 2008 12, 26 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7QLn2ea032701 12, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 16:49:02 -0500 12, 26 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 12, 26 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id B72FD182DA 12, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 14:49:01 -0700 (PDT) 12, 26 -- X-Ubc-Received: from [137.82.136.201] (robin.botany.ubc.ca [137.82.136.201]) 12, 26 -- by smtp.interchange.ubc.ca 12, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 12, 26 -- with ESMTPSA id {0K68009MT9XOVL-at-smtp.interchange.ubc.ca} for 12, 26 -- microscopy-at-microscopy.com; Tue, 26 Aug 2008 14:49:01 -0700 (PDT) 12, 26 -- Date: Tue, 26 Aug 2008 14:48:59 -0700 12, 26 -- From: Robin Young {youngre-at-interchange.ubc.ca} 12, 26 -- Subject: Epitope tagging for TEM in plants 12, 26 -- To: microscopy-at-microscopy.com 12, 26 -- Message-id: {971A04B5-FC09-4914-BE71-D7C2F647E395-at-interchange.ubc.ca} 12, 26 -- MIME-version: 1.0 (Apple Message framework v753.1) 12, 26 -- X-Mailer: Apple Mail (2.753.1) 12, 26 -- Content-type: text/plain; format=flowed; delsp=yes; charset=US-ASCII 12, 26 -- Content-transfer-encoding: 7bit 12, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.8.26.213405 12, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 12, 26 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_2000_2999 0, BODY_SIZE_5000_LESS 0, __C230066_P1_5 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 12, 26 -- X-Spam-Level: 12, 26 -- X-Spam-Flag: No ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both meulia.1-at-osu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: meulia.1-at-osu.edu Name: Tea Meulia
Organization: The Ohio State University
Title-Subject: [Filtered] help with identifying structures in SEM
Question: Dear Listserver members,
A plant pathology student took some sem images of 'diseased' honey locust tree internode bark, and found some curly structures, that we are not sure what they are. We would really appreciate, if anyone with more experience in recognizing plant tissues or pathogens, could help us to identify these structures. I have put these images on the web for you to view: http://www.oardc.ohio-state.edu/mcic/honey_locust/index.html
Thank you.
Tea
*************************************** Tea Meulia, PhD Research Scientists and Director Molecular and Cellular Imaging Center The Ohio State University/OARDC 1680 Madison Ave. Wooster OH 44691
I am looking into installing a cryo-ultramicrotome in a room that has some vibration issues. Our testing shows that the building itself is decent, but movement of people in the lab leads to significant vibrations (apparently the floor is wood). I'm interested to hear if anybody has experience with vibration dampening in this kind of situation, especially for cryo work. In particular, we're considering an active vibration dampening plate (The TS150 from HWL) or an air table, so it would be useful to hear if anybody has experience with these or other solutions.
Any input will be greatly appreciated.
Thanks, Eli
Eli Sone Institute of Biomaterials and Biomedical Engineering (IBBME) University of Toronto Rosebrugh Building, Room 405 164 College St. Toronto, Ontario M5S 3G9
If there is no one on the floor above you. Hanging the support for the cryo-ultramicrotome from the ceiling may help. Engineering the members the support hangs from is a complex endeavor and the next floor if there is one has to be noise free.
It may be an inexpensive solution if you have he right conditions.
The other way to isolate something from the floor is to bore hoes in it for supports that go to the basement. Occasionally it might be a solution for some labs on first floor. But it would be too expensive for most.
Gordon couger Stillwater, OK
----- Original Message ---- X-from: "eli.sone-at-utoronto.ca" {eli.sone-at-utoronto.ca}
Hi All,
I am looking into installing a cryo-ultramicrotome in a room that has some vibration issues. Our testing shows that the building itself is decent, but movement of people in the lab leads to significant vibrations (apparently the floor is wood). I'm interested to hear if anybody has experience with vibration dampening in this kind of situation, especially for cryo work. In particular, we're considering an active vibration dampening plate (The TS150 from HWL) or an air table, so it would be useful to hear if anybody has experience with these or other solutions.
Any input will be greatly appreciated.
Thanks, Eli
Eli Sone Institute of Biomaterials and Biomedical Engineering (IBBME) University of Toronto Rosebrugh Building, Room 405 164 College St. Toronto, Ontario M5S 3G9
Reminds me of the time as a kid I saw a record player (yes, big black shiny disk things, remember?) on a fairground "waltzer", hanging by bungee cords. The record didn't jump at all, even though everything around was shaking itself to bits...
Richard
gcouger-at-science-info.net wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Eli, } } If there is no one on the floor above you. Hanging the support for the cryo-ultramicrotome from the ceiling may help. Engineering the members the support hangs from is a complex endeavor and the next floor if there is one has to be noise free. } } It may be an inexpensive solution if you have he right conditions. } } The other way to isolate something from the floor is to bore hoes in it for supports that go to the basement. Occasionally it might be a solution for some labs on first floor. But it would be too expensive for most. } } Gordon couger } Stillwater, OK } } } } ----- Original Message ---- } X-from: "eli.sone-at-utoronto.ca" {eli.sone-at-utoronto.ca} } } Hi All, } } I am looking into installing a cryo-ultramicrotome in a room that has } some vibration issues. Our testing shows that the building itself is } decent, but movement of people in the lab leads to significant } vibrations (apparently the floor is wood). I'm interested to hear if } anybody has experience with vibration dampening in this kind of } situation, especially for cryo work. In particular, we're considering } an active vibration dampening plate (The TS150 from HWL) or an air } table, so it would be useful to hear if anybody has experience with } these or other solutions. } } Any input will be greatly appreciated. } } Thanks, } Eli } } Eli Sone } Institute of Biomaterials and Biomedical Engineering (IBBME) } University of Toronto } Rosebrugh Building, Room 405 } 164 College St. } Toronto, Ontario M5S 3G9 } } phone: 416-978-7422 } fax: 416-978-4317 } Email: eli.sone-at-utoronto.ca } } ==============================Original Headers============================== } 14, 16 -- From gcouger-at-science-info.net Thu Aug 28 01:59:02 2008 } 14, 16 -- Received: from web404.biz.mail.mud.yahoo.com (web404.biz.mail.mud.yahoo.com [68.142.201.35]) } 14, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m7S6x1px009363 } 14, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2008 01:59:01 -0500 } 14, 16 -- Received: (qmail 13382 invoked by uid 60001); 28 Aug 2008 06:59:01 -0000 } 14, 16 -- X-YMail-OSG: hC9eHn8VM1m6gerwkmnR9OqEOmnNRIseJzYEc6tNi82nHEkquhwL7HA8FSvR2fqNVNsQAaT8e2unKWSWyeG1l7IdMliJ8DZkKwnxK9dSuAxEK3.XAIb_QU35KE5Bmga5iT2e0ytg959lnOEjf4NQApE- } 14, 16 -- Received: from [66.103.115.181] by web404.biz.mail.mud.yahoo.com via HTTP; Wed, 27 Aug 2008 23:59:01 PDT } 14, 16 -- X-Mailer: YahooMailRC/1042.40 YahooMailWebService/0.7.218.2 } 14, 16 -- Date: Wed, 27 Aug 2008 23:59:01 -0700 (PDT) } 14, 16 -- From: Gordon Couger {gcouger-at-science-info.net} } 14, 16 -- Reply-To: Gordon Couger {gcouger-at-science-info.net} } 14, 16 -- Subject: Re: [Microscopy] ultramicrotomy - vibration dampening } 14, 16 -- To: eli.sone-at-utoronto.ca, microscopy-at-microscopy.com } 14, 16 -- MIME-Version: 1.0 } 14, 16 -- Content-Type: text/plain; charset=us-ascii } 14, 16 -- Message-ID: {571674.12219.qm-at-web404.biz.mail.mud.yahoo.com} } ==============================End of - Headers============================== } }
==============================Original Headers============================== 4, 25 -- From rb258-at-hermes.cam.ac.uk Thu Aug 28 04:28:50 2008 4, 25 -- Received: from ppsw-5.csi.cam.ac.uk (ppsw-5.csi.cam.ac.uk [131.111.8.135]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7S9SmwB028702 4, 25 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2008 04:28:49 -0500 4, 25 -- X-Cam-AntiVirus: no malware found 4, 25 -- X-Cam-SpamDetails: not scanned 4, 25 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 4, 25 -- Received: from canigou.msm.cam.ac.uk ([131.111.102.43]:1544) 4, 25 -- by ppsw-5.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.155]:587) 4, 25 -- with esmtpsa (PLAIN:rb258) (TLSv1:DHE-RSA-AES256-SHA:256) 4, 25 -- id 1KYdo6-0007Jt-GP (Exim 4.70) 4, 25 -- (return-path {rb258-at-hermes.cam.ac.uk} ); Thu, 28 Aug 2008 10:28:46 +0100 4, 25 -- Message-ID: {48B67164.1080800-at-integrityscientific.com} 4, 25 -- Date: Thu, 28 Aug 2008 10:35:32 +0100 4, 25 -- From: Richard Beanland {contact-at-integrityscientific.com} 4, 25 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 4, 25 -- MIME-Version: 1.0 4, 25 -- To: microscopy-at-microscopy.com 4, 25 -- CC: gcouger-at-science-info.net 4, 25 -- Subject: Re: [Microscopy] Re: ultramicrotomy - vibration dampening 4, 25 -- References: {200808280705.m7S75qnB019352-at-ns.microscopy.com} 4, 25 -- In-Reply-To: {200808280705.m7S75qnB019352-at-ns.microscopy.com} 4, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 25 -- Content-Transfer-Encoding: 7bit 4, 25 -- Sender: "Dr R. Beanland" {rb258-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
For those who do soil sample preparation, specifically thin sections, how do you do it if your samples are rich with salt? I would like to get your suggestions as I am to prepare consolidated and unconsolidated samples which, as I am told, react with the resin during impregnation and tend to give plucked sections later on.
Thanks in advance,
Melina Miralles
The Petroleum Institute Abu Dhabi, UAE
==============================Original Headers============================== 7, 31 -- From mmiralles-at-pi.ac.ae Thu Aug 28 06:47:46 2008 7, 31 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7SBlfwm017167 7, 31 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2008 06:47:43 -0500 7, 31 -- X-IronPort-AV: E=Sophos;i="4.32,286,1217793600"; 7, 31 -- d="scan'208";a="704751" 7, 31 -- Received: from unknown (HELO PI-EXF.PI.AC.AE) ([192.168.2.11]) 7, 31 -- by mx1.pi.ac.ae with ESMTP; 28 Aug 2008 15:34:57 +0400 7, 31 -- Received: from pi-exm.PI.AC.AE ([10.248.1.18]) by PI-EXF.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 7, 31 -- Thu, 28 Aug 2008 15:47:29 +0400 7, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; 7, 31 -- charset="us-ascii" 7, 31 -- x-cr-hashedpuzzle: Ap8i AqT6 Avtx A+Dr CVmL Diq3 D1Sj FmnB F4bP HHy0 HOTl Hm33 ILbA IUUE IdcX Kf+w;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{7119977C-6B48-4A32-93A9-2AEB2A30AF97};bQBtAGkAcgBhAGwAbABlAHMAQABwAGkALgBhAGMALgBhAGUA;Thu, 28 Aug 2008 11:47:26 GMT;VABoAGkAbgAgAFMAZQBjAHQAaQBvAG4AcwA= 7, 31 -- x-cr-puzzleid: {7119977C-6B48-4A32-93A9-2AEB2A30AF97} 7, 31 -- Content-class: urn:content-classes:message 7, 31 -- Subject: Thin Sections 7, 31 -- Date: Thu, 28 Aug 2008 15:47:26 +0400 7, 31 -- Message-ID: {D5603421C6303A46883F87E7968F285B04F36ABA-at-pi-exm.PI.AC.AE} 7, 31 -- In-Reply-To: {200808242220.m7OMKpb9013533-at-ns.microscopy.com} 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- Thread-Topic: Thin Sections 7, 31 -- Thread-Index: AckGN6/Tp5X4aKeoTIy5bZjhkvPFoQCyxhCQ 7, 31 -- References: {200808242220.m7OMKpb9013533-at-ns.microscopy.com} 7, 31 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 7, 31 -- To: {microscopy-at-microscopy.com} 7, 31 -- X-OriginalArrivalTime: 28 Aug 2008 11:47:29.0999 (UTC) FILETIME=[D9C68DF0:01C90903] 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m7SBlfwm017167 ==============================End of - Headers==============================
Dear List, Can someone tell me how to get some tobacco mosaic virus to make frozen-hydrated specimens with? TIA. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Thu Aug 28 11:28:32 2008 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7SGSVEx010817 2, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2008 11:28:32 -0500 2, 20 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by earth-doxen-postvirus (Postfix) with ESMTP id 317EC66E01F8 2, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2008 09:28:31 -0700 (PDT) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 2, 20 -- by earth-doxen-ssl (Postfix) with ESMTP id 34C4866E09AB 2, 20 -- for {microscopy-at-microscopy.com} ; Thu, 28 Aug 2008 09:28:26 -0700 (PDT) 2, 20 -- Message-Id: {BDFF8B4C-E7F4-48E1-BF96-4128DE415CE0-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v926) 2, 20 -- Subject: TMV 2, 20 -- Date: Thu, 28 Aug 2008 09:28:25 -0700 2, 20 -- X-Mailer: Apple Mail (2.926) ==============================End of - Headers==============================
We have occasional requests for cryoTEM that we cannot accommodate. Is there anyone who will accept outside samples? What would be the anticipated cost for a sample? I know that is hard to judge but we need a ball-park figure for planning purposes.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 3, 29 -- From dsherman-at-purdue.edu Fri Aug 29 09:36:29 2008 3, 29 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7TEaTa1029756 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 09:36:29 -0500 3, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 3, 29 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m7TEaSUk032714 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 10:36:28 -0400 3, 29 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 3, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m7TEaR38017909 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 10:36:28 -0400 3, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23] RDNS failed) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 3, 29 -- Fri, 29 Aug 2008 10:36:27 -0400 3, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 3, 29 -- Fri, 29 Aug 2008 14:36:06 +0000 3, 29 -- User-Agent: Microsoft-Entourage/12.12.0.080729 3, 29 -- Date: Fri, 29 Aug 2008 10:36:05 -0400 3, 29 -- Subject: CryoTEM service 3, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 3, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {C4DD8195.32B98%dsherman-at-purdue.edu} 3, 29 -- Thread-Topic: CryoTEM service 3, 29 -- Thread-Index: AckJ5JEyFWt3uhieRt+hhk9Cxw2vLQ== 3, 29 -- Mime-version: 1.0 3, 29 -- Content-type: text/plain; 3, 29 -- charset="US-ASCII" 3, 29 -- Content-transfer-encoding: 7bit 3, 29 -- X-OriginalArrivalTime: 29 Aug 2008 14:36:27.0783 (UTC) FILETIME=[9EC76170:01C909E4] 3, 29 -- X-PMX-Version: 5.4.0.320885 3, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
Years ago, when I required plant viral material (tomato bushy stunt in my case), I got it from Dr. A. Brunt (Glasshouse Crops Research Institute, Littlehampton, BN16 3PU, UK). But then I got only the inoculum, and as I required a significant amount of viral material, I had to infect plants, purify, etc. I think I also got some TMV from Dr. Jo Butler (http://www2.mrc-lmb.cam.ac.uk/SS/Butler_J/). Anyhow, this was in the UK and especially now with all that worries about biological warfare, may be it is safer if you locate a lab in the USA working with TMV.
May I ask you what kind of experiments are you planning? I worked for a while with different strains that showed a very different structural strength (actually the interactions giving rise to the virion were weaker in some of the mutants, as was evident by differential scanning calorimetry). The particles could be seen as broken or bended, by transmission microscopy, may be due to surface tension forces acting on the particles. Dr. RSS Fraser (r.fraser-at-sgm.ac.uk), Chief Executive of the Society for General Microbiology, was the person working (at that time) with these mutants.
Yours,
Antonio D. Molina-García
Instituto del Frío (CSIC) José Antonio Nováis, 10 Ciudad Universitaria 28040 Madrid
----- Original Message ----- X-from: {tivol-at-caltech.edu} To: {ifrm111-at-if.csic.es} Sent: Thursday, August 28, 2008 6:29 PM
Hi, Debbie
Try Brigitte P Sternberg {brigittnanoanalytical-at-yahoo.com} . As I remember, her website is www.nanoanalytical.com. She does great work.
Barbara Foster, President Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
NEWS! Come visit the NEW and IMPROVED www.MicroscopyEducation.com!
And don't forget: MME is now scheduling customized, on-site courses through Dec 2008. Call me for a free assessment and quote.
At 10:36 AM 8/29/2008, dsherman-at-purdue.edu wrote:
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Dear List, Thanks to the many people who suggested sources for TMV. I should have no trouble now getting what I need. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Fri Aug 29 11:49:36 2008 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7TGnaGx010175 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 11:49:36 -0500 2, 20 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by fire-doxen-postvirus (Postfix) with ESMTP id D3BEB328930 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 09:49:35 -0700 (PDT) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 2, 20 -- by fire-doxen-ssl (Postfix) with ESMTP id B8533328926 2, 20 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 09:49:34 -0700 (PDT) 2, 20 -- Message-Id: {800DFBB1-348F-4314-B0CA-EBCEC6C7E403-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v926) 2, 20 -- Subject: Thanks to all 2, 20 -- Date: Fri, 29 Aug 2008 09:49:34 -0700 2, 20 -- X-Mailer: Apple Mail (2.926) ==============================End of - Headers==============================
Hello, I have an older Zeiss SEM that was a donation. The phosphor screen at the end of the light pipe has been partially rubbed off. I notice that there are several vendors that sell small scintillator discs that are inexpensive. We do not have the funds at the moment to replace the whole light pipe or have it re-coated. Can the small scintillator discs be epoxyed right to the end of the light pipe? If so, is there a preferable type of epoxy?
Thanks, Erik
Dr. Erik McCullen Senior Research Scientist
==============================Original Headers============================== 6, 19 -- From mccullen-at-indigo.eng.wayne.edu Fri Aug 29 13:10:10 2008 6, 19 -- Received: from mxout.eng.wayne.edu (mxout.eng.wayne.edu [141.217.202.35]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m7TIA9cv026727 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 13:10:09 -0500 6, 19 -- Received: from Erik ([141.217.43.150]) 6, 19 -- by mxout.eng.wayne.edu (8.12.9/8.12.9) with ESMTP id m7TIA666014433 6, 19 -- for {microscopy-at-microscopy.com} ; Fri, 29 Aug 2008 14:10:09 -0400 (EDT) 6, 19 -- From: "Erik" {mccullen-at-indigo.eng.wayne.edu} 6, 19 -- To: {microscopy-at-microscopy.com} 6, 19 -- Subject: SEM phosphor screen 6, 19 -- Date: Fri, 29 Aug 2008 14:10:09 -0400 6, 19 -- Message-ID: {026001c90a02$7c402240$4c0415ac-at-Erik} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: text/plain; 6, 19 -- charset="us-ascii" 6, 19 -- Content-Transfer-Encoding: 7bit 6, 19 -- X-Mailer: Microsoft Office Outlook 11 6, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 6, 19 -- Thread-Index: AckKAnkHgtzEUgQHQCm1sAFPouwDyg== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wim.vandenbroeck-at-UGent.be as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wim.vandenbroeck-at-UGent.be Name: Wim Van den Broeck
Organization: Department of Morphology, Ghent University
Title-Subject: [Filtered] LEICA EM tissue processor: protocols
Question: Dear Friends,
We have just bought the LEICA EM Tissue Processor (automated routine tissue processor), and are looking for some suitable protocols to embed the following tissues in Spurrís resin:
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Email: Wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] TEM HR mode
Question: I would like to say thank you to everybody who replied to my posting. Your advice and suggestions were very helpful. Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both friess-at-limedion.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: friess-at-limedion.de Name: Frank
Title-Subject: [Filtered] JEOL serial port
Question: Hello,
I am still searching for someone who has a JEOL 6300F, 6400F or 6600F with activated RS232 serial port via EPROMS. I am right now coding software to digitize our SEM with a ADDA-Card. The card is pretty fast, so I get a pretty good live scan picture and I would like to use the serial port to do autofocusing and to correct astigmatism automatically. I would be very happy if someone could help me.
Greetings Robin, Yours is clearly a complex issue with many things to consider, but here are two:
Try trouble shooting at the LM level. That means for whatever embedment you do (well probably not epoxy) cut some ~2 um semi-then sections, stain with primary and a fluorescent secondary and check in fluorescence. If you don't have signal there it is probably not going to work in TEM (yes, there are exceptions, but...) and you can do LM work a lot faster than TEM.
Try doing your freeze sub in acetone only. Rembarkably, plant cells are pretty well fixed in acetone only during freeze sub. Indeed, not quite as good as with osmium and glut in there too, but pure acetone is very favorable for antigenicity. You may find the trade off worth it.
Hope this helps. Good luck.
Tobias
} } } Hello all, } } We are having some trouble in our lab getting immunogold labeling on } epitope tagged materials to work. Here's the background: } } We have a number of different projects on the go, all of which we } would like to use epitope tagged proteins for immunogold labeling. We } have 3 different proteins we've labelled, 2 of which have YFP, one of } which has a Myc tag, and another (which we have not tried yet in TEM) } which has a His-tag. In each case the label can be localized using } other methods. In the case of the YFP-tag, the label can be seen } using live cell imaging, and in the case of the Myc and His-tags, } Western blots confirm the presence of the tagged protein. } } Our problem is getting the protein to label sections prepared for } TEM. Our standard procedure for immunolabelling is to high pressure } freeze, freeze substitute in 0.25% glut, 2% UA in acetone, and then } embed in LR White at room temperature. In the case of the YFP label } we could see label, but there seems to be no consistency or pattern } that we could discern, especially compared to our live cell results. } In the case of the Myc tag, we can get no label whatsoever, using a } few different anti-Myc labels. } } Because we were having trouble with the Myc label, we decided to try } several different preservation techniques. We tried low-temperature } embedding in lowicryl HM20 or K4M, and in both cases the waxy cuticle } of our arabidopsis stems (and seeds) resulted in the samples simply } falling out of the blocks. Our only explanation is that the waxes } were so well preserved by these methods that they didn't allow for } proper preservation. According to others, this is reminiscent of the } problem that drosophila experts have when embedding embryos that have } developed a cuticle. } } We have also tried etching both our LR white samples, and osmicated } samples (embedded in Spurr's) prior to labelling, with no success. } } My question to you is whether anyone has a tried and true method for } embedding difficult samples for for immunolabelling? We're desperate } at this point, and willing to try almost anything. Our samples } (Arabidopsis stems and developing seeds) have waxy cuticles that are } both important to our analysis and part of the reason we have so much } trouble embedding our material. We have used a number of different } epitope tags and antibodies, with virtually no success, which is why } we are currently trying to optimize our embedding techniques, rather } than trying different epitopes tags. } } Thanks in advance for any insight that you might have. I'm sorry that } this is so long. } Cheers, } Robin } } } Robin Young, M.Sc. } PhD Candidate, Samuels and Haughn Labs } Botany Dept, University of British Columbia } 6270 University Blvd, Vancouver, BC } V6K 1E4 / Fax: 604.822.6089 } } } } ==============================Original Headers============================== } 12, 26 -- From youngre-at-interchange.ubc.ca Tue Aug 26 16:49:02 2008 } 12, 26 -- Received: from mr3.mail-relay.ubc.ca } (mr3.mail-relay.ubc.ca [137.82.45.5]) } 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m7QLn2ea032701 } 12, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 } 16:49:02 -0500 } 12, 26 -- Received: from mta2.interchange.ubc.ca } (mta2.interchange.ubc.ca [142.103.145.70]) } 12, 26 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id B72FD182DA } 12, 26 -- for {microscopy-at-microscopy.com} ; Tue, 26 Aug 2008 } 14:49:01 -0700 (PDT) } 12, 26 -- X-Ubc-Received: from [137.82.136.201] (robin.botany.ubc.ca } [137.82.136.201]) } 12, 26 -- by smtp.interchange.ubc.ca } 12, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) } 12, 26 -- with ESMTPSA id {0K68009MT9XOVL-at-smtp.interchange.ubc.ca} for } 12, 26 -- microscopy-at-microscopy.com; Tue, 26 Aug 2008 14:49:01 -0700 (PDT) } 12, 26 -- Date: Tue, 26 Aug 2008 14:48:59 -0700 } 12, 26 -- From: Robin Young {youngre-at-interchange.ubc.ca} } 12, 26 -- Subject: Epitope tagging for TEM in plants } 12, 26 -- To: microscopy-at-microscopy.com } 12, 26 -- Message-id: } {971A04B5-FC09-4914-BE71-D7C2F647E395-at-interchange.ubc.ca} } 12, 26 -- MIME-version: 1.0 (Apple Message framework v753.1) } 12, 26 -- X-Mailer: Apple Mail (2.753.1) } 12, 26 -- Content-type: text/plain; format=flowed; delsp=yes; charset=US-ASCII } 12, 26 -- Content-transfer-encoding: 7bit } 12, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.1.325704, } Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.8.26.213405 } 12, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca } 12, 26 -- X-PerlMx-Spam: Probability=7%, Report=BODY_SIZE_2000_2999 } 0, BODY_SIZE_5000_LESS 0, __C230066_P1_5 0, __C230066_P5 0, __CT 0, } __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, } __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 } 12, 26 -- X-Spam-Level: } 12, 26 -- X-Spam-Flag: No } ==============================End of - Headers==============================
I honestly ask all the German listers if there is anybody owning a (short and/or long) instruction manual WRITTEN in GERMAN how to work (and get started) with a JEOL TEM-JEM 1010.
If there is a print only, I should be glad and really appreciate receiving a copy which I am able to scan in for another colleague in urgent need of such a manual (he only has an English version, which seems pretty tricky to be read / for starting work with that machine).
Apologize if I bother you with this request.
Thanking in advance,
Best wishes and regards
Wolfgang MUSS EM-Lab, Salzburg-Austria
==============================Original Headers============================== 11, 35 -- From W.Muss-at-salk.at Mon Sep 1 12:22:33 2008 11, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m81HMXIX000443 11, 35 -- for {Microscopy-at-Microscopy.Com} ; Mon, 1 Sep 2008 12:22:33 -0500 11, 35 -- Received: from localhost (localhost [127.0.0.1]) 11, 35 -- by hermes.salk.at (Postfix) with ESMTP id 8D386C385A 11, 35 -- for {Microscopy-at-Microscopy.Com} ; Mon, 1 Sep 2008 19:22:31 +0200 (CEST) 11, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 11, 35 -- Received: from hermes.salk.at ([127.0.0.1]) 11, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 11, 35 -- with ESMTP id Ugh5pNLddQhD for {Microscopy-at-Microscopy.Com} ; 11, 35 -- Mon, 1 Sep 2008 19:22:31 +0200 (CEST) 11, 35 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 11, 35 -- by hermes.salk.at (Postfix) with ESMTP id 3CD8AC3843 11, 35 -- for {Microscopy-at-Microscopy.Com} ; Mon, 1 Sep 2008 19:22:31 +0200 (CEST) 11, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 11, 35 -- Mon, 1 Sep 2008 19:22:30 +0200 11, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 35 -- Content-class: urn:content-classes:message 11, 35 -- MIME-Version: 1.0 11, 35 -- Content-Type: text/plain; 11, 35 -- charset="us-ascii" 11, 35 -- Subject: TEM: to the German Colleagues: manual (long, short) IN GERMAN for JEOL JEM 1010 urgently needed 11, 35 -- Date: Mon, 1 Sep 2008 19:22:30 +0200 11, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D045A-at-N1RZ116.lksdom21.lks.local} 11, 35 -- X-MS-Has-Attach: 11, 35 -- X-MS-TNEF-Correlator: 11, 35 -- Thread-Topic: TEM: to the German Colleagues: manual (long, short) IN GERMAN for JEOL JEM 1010 urgently needed 11, 35 -- Thread-Index: AckMV1BrHNIss/dOSwamARE1gfViqg== 11, 35 -- From: "Wolfgang Muss" {W.Muss-at-salk.at} 11, 35 -- To: {Microscopy-at-Microscopy.Com} 11, 35 -- X-OriginalArrivalTime: 01 Sep 2008 17:22:31.0094 (UTC) FILETIME=[509D3D60:01C90C57] 11, 35 -- X-Scanned-By: SALK-Content-Filter 11, 35 -- Content-Transfer-Encoding: 8bit 11, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m81HMXIX000443 ==============================End of - Headers==============================
Dear cryo-colleagues! Related to my last question on the list, dealing with the preparation of frozen samples for TEM, I experienced that 5% water in acetone, once placed at -80°C, will freeze at the bottom of the tube. I am very inexperienced in High-Pressure Freezing and Freeze substitution, but I know that Paul Walther proposed to mix water with acetone to improve membrane visualization. Well I suppose that the mix is first cooled down to temperature, so this means that water separates from acetone in the mix! Something must be wrong with my argumentation, but I don't see what/where. Can someone please help me? Best regards,
Stephane
==============================Original Headers============================== 5, 21 -- From nizets2-at-yahoo.com Tue Sep 2 04:39:33 2008 5, 21 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m829dX1M012612 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 04:39:33 -0500 5, 21 -- Received: (qmail 26838 invoked by uid 60001); 2 Sep 2008 09:39:33 -0000 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 21 -- s=s1024; d=yahoo.com; 5, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 5, 21 -- b=V/MWD0f02Q4blpZGMfu5wAzNlNFReNU89ZZ2fTDsSFXT2Fahp4xDZXZmjOTSpRy5t1/FZPlFvp/CELOHYuXfZF3fm7vzMtT2s+jokjn2WATJxFZ3DxPedLcM3TOjlx5svXb7i32IeXNZpgnrfTh8nPAQLD1z1v+V+MWqoQIeHsU=; 5, 21 -- X-YMail-OSG: q8JqZjkVM1l.ziUnFAD49PNxWNaLXZQecB697eUnsUUHIElkIsaYfq5US_zXcRShSaAhsE31DnxxLqczUxNJFxc_N3woi53eLC2l6Sp6kQ-- 5, 21 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Tue, 02 Sep 2008 02:39:33 PDT 5, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218.2 5, 21 -- Date: Tue, 2 Sep 2008 02:39:33 -0700 (PDT) 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 21 -- Subject: water in acetone? 5, 21 -- To: microscopy-at-microscopy.com 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 5, 21 -- Message-ID: {306950.26500.qm-at-web37408.mail.mud.yahoo.com} 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m829dX1M012612 ==============================End of - Headers==============================
The Robert P. Apkarian Integrated Electron Microscopy Core at Emory University is seeking to hire a full time staff member. The facility is a university-wide research center with both TEM and SEM capabilities. The facility is undergoing redevelopment and currently houses 2 120 kV TEMs (one with cryo-holder), 2 FESEMs (one with cryo- holder), high pressure freezer and freeze substitution system, several ultramicrotomes, and other conventional EM specimen preparation equipment. The candidate should have a Bachelor's degree in a scientific field or equivalent combination of experience, education, and training. Candidates should have an interest, and preferably 2 to 3 years of experience, in biological or materials electron microscopy, and should enjoy working as part of an energetic team. Experience/knowledge of immunocytochemistry and cryo techniques would be advantageous.
This position is immediately available. Interested individuals should contact either Hong Yi (404-712-8491, hyi-at-emory.edu) or Dr. Elizabeth Wright (erwrigh-at-emory.edu). Thank you.
Hong
============= Hong Yi Technical Director Robert P. Apkarian Integrated Electron Microscopy Core Suite E106 Cherry L. Emerson Hall 1515 Dickey Drive, NE Emory University Atlanta, GA 30322
Tel: (404) 712-8491 hyi-at-emory.edu
==============================Original Headers============================== 7, 22 -- From hyi-at-emory.edu Tue Sep 2 05:45:58 2008 7, 22 -- Received: from QMTA04.westchester.pa.mail.comcast.net (qmta04.westchester.pa.mail.comcast.net [76.96.62.40]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82Ajvrr028876 7, 22 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 05:45:58 -0500 7, 22 -- Received: from OMTA05.westchester.pa.mail.comcast.net ([76.96.62.43]) 7, 22 -- by QMTA04.westchester.pa.mail.comcast.net with comcast 7, 22 -- id 9meP1a0040vyq2s54mlxRV; Tue, 02 Sep 2008 10:45:57 +0000 7, 22 -- Received: from [71.236.31.48] ([71.236.31.48]) 7, 22 -- by OMTA05.westchester.pa.mail.comcast.net with comcast 7, 22 -- id 9mlx1a00E12HxMB3Rmlx6K; Tue, 02 Sep 2008 10:45:57 +0000 7, 22 -- X-Authority-Analysis: v=1.0 c=1 a=aHIqIkF06JLJQjovf9oA:9 7, 22 -- a=35UDV0cLpB135J5qYWYA:7 a=oiq37g6blscPFUgpuOrWtQXhR2QA:4 a=gUe8_R_abCcA:10 7, 22 -- a=6PlspQdYA9AA:10 a=9OHTkwyHC8cA:10 7, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Message-Id: {6BB0383D-7986-4FF4-8665-9DFC049E32AE-at-emory.edu} 7, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 22 -- To: microscopy-at-microscopy.com 7, 22 -- From: Hong Yi {hyi-at-emory.edu} 7, 22 -- Subject: Position opening 7, 22 -- Date: Tue, 2 Sep 2008 06:45:52 -0400 7, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
When we were setting up to do low-temperature substitution (mid-80s) I found some info detailing that water is only sparingly soluble in acetone at -80C (fraction of a percent or so), so we used molecular sieves in the bottom of the sample vials to constantly keep the acetone dry and able to dissolve more water from the sample; also don't overload the sample volume relative to acetone volume, and use properly dehydrated acetone for substitution fluid whether or not there is molecular sieve used. What you experience must be the proof of the low solubility. We did have very low tissue contrast with the dry acetone. Methanol is commonly used if higher concentrations of water are to be present for low-temperature substitution or subsequent UAc staining at low temps.
Dale
nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear cryo-colleagues! } Related to my last question on the list, dealing with the preparation of frozen samples for TEM, I experienced that 5% water in acetone, once placed at -80°C, will freeze at the bottom of the tube. I am very inexperienced in High-Pressure Freezing and Freeze substitution, but I know that Paul Walther proposed to mix water with acetone to improve membrane visualization. Well I suppose that the mix is first cooled down to temperature, so this means that water separates from acetone in the mix! } Something must be wrong with my argumentation, but I don't see what/where. } Can someone please help me? } Best regards, } } Stephane } } } } } } ==============================Original Headers============================== } 5, 21 -- From nizets2-at-yahoo.com Tue Sep 2 04:39:33 2008 } 5, 21 -- Received: from web37408.mail.mud.yahoo.com (web37408.mail.mud.yahoo.com [209.191.91.140]) } 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m829dX1M012612 } 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 04:39:33 -0500 } 5, 21 -- Received: (qmail 26838 invoked by uid 60001); 2 Sep 2008 09:39:33 -0000 } 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 5, 21 -- s=s1024; d=yahoo.com; } 5, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; } 5, 21 -- b=V/MWD0f02Q4blpZGMfu5wAzNlNFReNU89ZZ2fTDsSFXT2Fahp4xDZXZmjOTSpRy5t1/FZPlFvp/CELOHYuXfZF3fm7vzMtT2s+jokjn2WATJxFZ3DxPedLcM3TOjlx5svXb7i32IeXNZpgnrfTh8nPAQLD1z1v+V+MWqoQIeHsU=; } 5, 21 -- X-YMail-OSG: q8JqZjkVM1l.ziUnFAD49PNxWNaLXZQecB697eUnsUUHIElkIsaYfq5US_zXcRShSaAhsE31DnxxLqczUxNJFxc_N3woi53eLC2l6Sp6kQ-- } 5, 21 -- Received: from [80.122.101.100] by web37408.mail.mud.yahoo.com via HTTP; Tue, 02 Sep 2008 02:39:33 PDT } 5, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218.2 } 5, 21 -- Date: Tue, 2 Sep 2008 02:39:33 -0700 (PDT) } 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 5, 21 -- Subject: water in acetone? } 5, 21 -- To: microscopy-at-microscopy.com } 5, 21 -- MIME-Version: 1.0 } 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 } 5, 21 -- Message-ID: {306950.26500.qm-at-web37408.mail.mud.yahoo.com} } 5, 21 -- Content-Transfer-Encoding: 8bit } 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m829dX1M012612 } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 20 -- From dac-at-research.umass.edu Tue Sep 2 07:09:04 2008 4, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82C942A014770 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 07:09:04 -0500 4, 20 -- Received: from [192.168.1.101] (68-112-238-118.dhcp.oxfr.ma.charter.com [68.112.238.118]) 4, 20 -- (authenticated bits=0) 4, 20 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m82C93Ne002412 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 4, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 08:09:03 -0400 4, 20 -- Message-ID: {48BD3B50.7070301-at-research.umass.edu} 4, 20 -- Date: Tue, 02 Sep 2008 08:10:40 -0500 4, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 4, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.16) Gecko/20080702 SeaMonkey/1.1.11 4, 20 -- MIME-Version: 1.0 4, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 4, 20 -- Subject: Re: [Microscopy] water in acetone? 4, 20 -- References: {200809020945.m829jgTA021059-at-ns.microscopy.com} 4, 20 -- In-Reply-To: {200809020945.m829jgTA021059-at-ns.microscopy.com} 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
in our lab we have a TEM Jeol JEM-3010 used mainly for HREM studies. We are thinking of buying a new chiller, and we'd like to consider various brands, not only the current one which is still the one provided by Jeol at the installation of the instrument (Neslab). Obviously for HREM we are concerned with water temperature stability and vibrations.
What is your experience in this respect? Any advice or comment will be appreciated! Thanks
Check with your local JEOL office--in the USA they recommend Haskris with specific modifications for thermal and flow stability,etc. Our base unit is an R250 with options C, H, K, N2, R4 and S. We use this for our JEOL 4000.
Roseann
Roseann Csencsits LBL Donner Lab 365 1 Cyclotron Road Berkeley CA 94720 United States 510-486-4548
On Sep 2, 2008, at 6:52 AM, dcristofori-at-unive.it wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear ListServers, } } in our lab we have a TEM Jeol JEM-3010 used mainly for HREM studies. } We } are thinking of buying a new chiller, and we'd like to consider } various } brands, not only the current one which is still the one provided by } Jeol } at the installation of the instrument (Neslab). Obviously for HREM we } are concerned with water temperature stability and vibrations. } } What is your experience in this respect? Any advice or comment will be } appreciated! } Thanks } } Davide } } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive.it } } Universita' Ca' Foscari Venezia } Dipartimento di Chimica Fisica } Lab. di Scienza e Tecnologia dei Materiali } Via Torino, 155b } I-30172 Mestre (VE) } Italy
Dear List, Here are the replies I got regarding sources of TMV:
} Contact your nearest virologist in the Plant Pathology Dept. } Beth Richardson
} American Type Culture Collection (ATCC) sells many organisms, } including tobacco mosaic virus. } } http://www.atcc.org } } Maureen Petersen
} I have loads of it, got it from the greenhouses here at Cornell for } teaching purposes. I'm not sure if this is something I can send you } though. } } John Grazul } CCMR TEM Facility } Cornell University
} Contact one of the California Universities (like Riverside or Davis) } who } have a Plant Pathology Department. Most of them have someone doing } work } with TMV and they should be able to sent you a sample. } } Deborah
} Years ago, when I required plant viral material (tomato bushy stunt } in my case), I got it from Dr. A. Brunt (Glasshouse Crops Research } Institute, Littlehampton, BN16 3PU, UK). But then I got only the } inoculum, and as I required a significant amount of viral material, } I had to infect plants, purify, etc. I think I also got some TMV } from Dr. Jo Butler (http://www2.mrc-lmb.cam.ac.uk/SS/Butler_J/). } Anyhow, this was in the UK and especially now with all that worries } about biological warfare, may be it is safer if you locate a lab in } the USA working with TMV.
} Antonio D. Molina-García
} Contact Dr. Robert Gilbertson at UC Davis. He has his lab students do } purifications. } } rlgilbertson-at-ucdavis.edu } } Rick Harris
} Did your suggestions include contacting Gerald Stubbs {gerald.stubbs-at-vanderbilt.edu } } or Bridget Carragher {bcarr-at-scripps.edu} ? } } 17 years ago I requested and got an infected tobacco plant from } someone at NCSU in Raleigh, NC; you've reminded me to try to beg } another.
Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 20, 21 -- From tivol-at-caltech.edu Tue Sep 2 11:29:18 2008 20, 21 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 20, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82GTIks002714 20, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 11:29:18 -0500 20, 21 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 20, 21 -- by fire-doxen-postvirus (Postfix) with ESMTP id E21392E50B7B 20, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 09:29:17 -0700 (PDT) 20, 21 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 20, 21 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 20, 21 -- by fire-doxen-ssl (Postfix) with ESMTP id AEADF2E5028A 20, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 09:29:16 -0700 (PDT) 20, 21 -- Message-Id: {16CEFFD3-FE71-4E11-8444-EDD1C549F754-at-caltech.edu} 20, 21 -- From: Bill Tivol {tivol-at-caltech.edu} 20, 21 -- To: microscopy-at-microscopy.com 20, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 20, 21 -- Mime-Version: 1.0 (Apple Message framework v926) 20, 21 -- Subject: TMV--replies to my inquiry 20, 21 -- Date: Tue, 2 Sep 2008 09:29:16 -0700 20, 21 -- X-Mailer: Apple Mail (2.926) 20, 21 -- Content-Transfer-Encoding: 8bit 20, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m82GTIks002714 ==============================End of - Headers==============================
I took a bit more of a different approach, when the chiller to our ISI-40 stooped working effectively. The first thing I attempted was to have the chiller repaired, after $1,000 I found out that the unit's refrigeration was beyond repair, however the pump was working just fine. I purchased an Aquarium chiller and hooked it up to run inline with the pump. the chiller worked out great, especially with the digital temperature regulator. Moreover it has the added benefit of using standard refrigeration fluid (found at auto store) to replenish it. Perhaps this could solve your problems, however you should consider the type of material used within the chiller (possible contamination issues) and from my experience purchase one that has a bit more cooling power that you need (from what I found the advertised BTU lies about 20%-30%) and how much of a temperature tolerance you can allow. By no means this is a in any way a replacement for a popper chiller (such as Haskris ) however I found it to be a bit more cost effective for the age of my scope.
Best Regards, Anatoli Oleynik Umbrella Technologies, INC. toli-at-3dfs.com
spamMfilter-at-microscopy.com wrote: } Subject: REJECTED MAIL } X-Mailer: MicroscopyListSpam Mail Filter vNJZ-2005080908 } X-lewp: MicroscopyListSpam NAGS } } -------------------------------------------------------- } Your mail has been rejected for the following reason(s): } -------------------------------------------------------- } Other: } } Content-Type: multipart/alternative } } An Email Attachment was detected with your message!!! } The Microscopy Listserver will not accept any ATTACHMENTS as a safety measure. } Suspect or Possibly an Unregistered User Address: cell.toli-at-gmail.com } } Site match: mail.com } --------------------------------------------------------------------- } If you have a legitimate reason to contact this SITE, you may get your } mail through the filter by FORWARDing this Email together with } all the error messages and header linesto the Address: } } } } MicroscopyListSpamFilter-at-Microscopy.Com: } } --------------------------------------------------------------------- } } } MicroscopyList Email Filter vNJZ-2005080908 - Your Friendly Neighborhood SysOp } } The text of the rejected email follows: } ********************************************************************* } } This is a multi-part message in MIME format. } --------------040703090105090808070207 } Content-Type: text/plain; charset=ISO-8859-1; format=flowed } Content-Transfer-Encoding: quoted-printable } } Davide,=20 } } I took a bit more of a different approach, when the chiller to the ISI-40= } stooped working effectively.=20 } The first thing I attempted was to have the chiller repaired, after $1,00= } 0 I found out that the unit's refrigeration was beyond repair, however th= } e pump was working just fine. I purchased an Aquarium chiller and hooked = } it up to run inline with the pump. the chiller worked out great, especial= } ly with the digital temperature regulator. Moreover it has the added bene= } fit of using standard refrigeration fluid (found at auto store) to replen= } ish it. Perhaps this could solve your problems, however you should consid= } er the type of material used within the chiller (possible contamination i= } ssues) and from my experience purchase one that has a bit more cooling po= } wer that you need (from what I found the advertised BTU lies about 20%-30= } %) and how much of a temperature tolerance you can allow. By no means thi= } s is a in any way a replacement for a popper chiller (such as Haskris ) h= } owever I found it to be a bit more cost effective for the age of my scope= } =2E =20 } } } Best Regards,=20 } Anatoli Oleynik } Umbrella Technologies, INC. } toli-at-3dfs.com } } } } } } RCsencsits-at-lbl.gov wrote: } --| -----------------------------------------------------------------------= } ----- } --| The Microscopy ListServer -- CoSponsor: The Microscopy Society of Amer= } ica } --| To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListse= } rver } --| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --| -----------------------------------------------------------------------= } ----- } --| } --| Davide, } --| } --| Check with your local JEOL office--in the USA they recommend Haskris =20 } --| with specific modifications for thermal and flow stability,etc. Our =20 } --| base unit is an R250 with options C, H, K, N2, R4 and S. We use =20 } --| this for our JEOL 4000. } --| } --| Roseann } --| } --| Roseann Csencsits } --| LBL Donner Lab 365 } --| 1 Cyclotron Road } --| Berkeley CA 94720 } --| United States } --| 510-486-4548 } --| } --| On Sep 2, 2008, at 6:52 AM, dcristofori-at-unive.it wrote: } --| } --| =20 } --|--| } --|--| ----------------------------------------------------------------------= } ------ } --|--| The Microscopy ListServer -- CoSponsor: The Microscopy Society of =20 } --|--| America } --|--| To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyLists= } erver } --|--| On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } --|--| ----------------------------------------------------------------------= } ------ } --|--| } --|--| Dear ListServers, } --|--| } --|--| in our lab we have a TEM Jeol JEM-3010 used mainly for HREM studies. = } } --|--| We } --|--| are thinking of buying a new chiller, and we'd like to consider =20 } --|--| various } --|--| brands, not only the current one which is still the one provided by =20 } --|--| Jeol } --|--| at the installation of the instrument (Neslab). Obviously for HREM we } --|--| are concerned with water temperature stability and vibrations. } --|--| } --|--| What is your experience in this respect? Any advice or comment will be= } } --|--| appreciated! } --|--| Thanks } --|--| } --|--| Davide } --|--| } --|--| } --|--| } --|--| ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } --|--| Davide Cristofori } --|--| } --|--| direct phone =3D 041.234.67.26 } --|--| e-mail =3D dcristofori[at]unive.it } --|--| } --|--| Universita' Ca' Foscari Venezia } --|--| Dipartimento di Chimica Fisica } --|--| Lab. di Scienza e Tecnologia dei Materiali } --|--| Via Torino, 155b } --|--| I-30172 Mestre (VE) } --|--| Italy } --|--| =20 } --| =20 } --| =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= } =3D=3D=3D=3D=3D=3DOriginal Headers=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D } --| 6, 26 -- From RCsencsits-at-lbl.gov Tue Sep 2 11:04:03 2008 } --| 6, 26 -- Received: from ironport3.lbl.gov (ironport3.lbl.gov [128.3.41.= } 25]) } --| 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id = } m82G43qJ021072 } --| 6, 26 -- for |--Microscopy-at-microscopy.com--|; Tue, 2 Sep 2008 11:04:03 -05= } 00 } --| 6, 26 -- X-Ironport-SBRS: 2.9 } --| 6, 26 -- X-IronPort-Anti-Spam-Filtered: true } --| 6, 26 -- X-IronPort-Anti-Spam-Result: At8CAM4AvUiAAykMgWdsb2JhbACSTgEBE= } CAFmjIJhhwBa30 } --| 6, 26 -- X-IronPort-AV: E=3DSophos;i=3D"4.32,317,1217833200";=20 } --| 6, 26 -- d=3D"scan'208";a=3D"4507340" } --| 6, 26 -- Received: from mta2.lbl.gov ([128.3.41.12]) } --| 6, 26 -- by ironport3.lbl.gov with ESMTP; 02 Sep 2008 09:04:02 -0700 } --| 6, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17= } -f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) } --| 6, 26 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id m82G41SE015983;= } } --| 6, 26 -- Tue, 2 Sep 2008 09:04:01 -0700 (PDT) } --| 6, 26 -- Cc: Microscopy-at-microscopy.com } --| 6, 26 -- Message-Id: |--9EF31184-F3B3-4F9F-859A-D401429DF162-at-lbl.gov--| } --| 6, 26 -- From: Roseann Csencsits |--RCsencsits-at-lbl.gov--| } --| 6, 26 -- To: dcristofori-at-unive.it } --| 6, 26 -- In-Reply-To: |--200809021352.m82DqQ17012174-at-ns.microscopy.com--| } --| 6, 26 -- Content-Type: text/plain; charset=3DUS-ASCII; format=3Dflowed;= } delsp=3Dyes } --| 6, 26 -- Content-Transfer-Encoding: 7bit
} --| 6, 26 -- Mime-Version: 1.0 (Apple Message framework v926) } --| 6, 26 -- Subject: Re: [Microscopy] chiller for Jeol TEM JEM-3010 } --| 6, 26 -- Date: Tue, 2 Sep 2008 09:03:59 -0700 } --| 6, 26 -- References: |--200809021352.m82DqQ17012174-at-ns.microscopy.com--| } --| 6, 26 -- X-Mailer: Apple Mail (2.926) } --| =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= } =3D=3D=3D=3D=3D=3DEnd of - Headers=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D } --| } --| __________ Information from ESET Smart Security, version of virus signa= } ture database 3408 (20080902) __________ } --| } --| The message was checked by ESET Smart Security. } --| } --| http://www.eset.com } --| } --| } --| } --| =20 } } } --------------040703090105090808070207 } Content-Type: text/html; charset=ISO-8859-1 } Content-Transfer-Encoding: 7bit } } |--!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN"--| } |--html--| } |--head--| } |--meta content="text/html;charset=ISO-8859-1" http-equiv="Content-Type"--| } |--title--||--/title--| } |--/head--| } |--body bgcolor="#ffffff" text="#000000"--| } |--pre wrap=""--|Davide, } } I took a bit more of a different approach, when the chiller to the ISI-40 stooped working effectively. } The first thing I attempted was to have the chiller repaired, after $1,000 I found out that the unit's refrigeration was beyond repair, however the pump was working just fine. I purchased an Aquarium chiller and hooked it up to run inline with the pump. the chiller worked out great, especially with the digital temperature regulator. Moreover it has the added benefit of using standard refrigeration fluid (found at auto store) to replenish it. Perhaps this could solve your problems, however you should consider the type of material used within the chiller (possible contamination issues) and from my experience purchase one that has a bit more cooling power that you need (from what I found the advertised BTU lies about 20%-30%) and how much of a temperature tolerance you can allow. By no means this is a in any way a replacement for a popper chiller (such as Haskris ) however I found it to be a bit more cost effective for the age of my scope. } } } Best Regards, } Anatoli Oleynik } Umbrella Technologies, INC. } |--a class="moz-txt-link-abbreviated" href="mailto:toli-at-3dfs.com"--|toli-at-3dfs.com|--/a--| } } } |--/pre--| } |--br--| } |--br--| } |--a class="moz-txt-link-abbreviated" href="mailto:RCsencsits-at-lbl.gov"--|RCsencsits-at-lbl.gov|--/a--| wrote: } |--blockquote cite="mid:200809021608.m82G8j0a028589-at-ns.microscopy.com" } type="cite"--| } |--pre wrap=""--|---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- |--a class="moz-txt-link-freetext" href="http://www.microscopy.com/MicroscopyListserver"--|http://www.microscopy.com/MicroscopyListserver|--/a--| } On-Line Help |--a class="moz-txt-link-freetext" href="http://www.microscopy.com/MicroscopyListserver/FAQ.html"--|http://www.microscopy.com/MicroscopyListserver/FAQ.html|--/a--| } ---------------------------------------------------------------------------- } } Davide, } } Check with your local JEOL office--in the USA they recommend Haskris } with specific modifications for thermal and flow stability,etc. Our } base unit is an R250 with options C, H, K, N2, R4 and S. We use } this for our JEOL 4000. } } Roseann } } Roseann Csencsits } LBL Donner Lab 365 } 1 Cyclotron Road } Berkeley CA 94720 } United States } 510-486-4548 } } On Sep 2, 2008, at 6:52 AM, |--a class="moz-txt-link-abbreviated" href="mailto:dcristofori-at-unive.it"--|dcristofori-at-unive.it|--/a--| wrote: } } |--/pre--| } |--blockquote type="cite"--| } |--pre wrap=""--| } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- |--a class="moz-txt-link-freetext" href="http://www.microscopy.com/MicroscopyListserver"--|http://www.microscopy.com/MicroscopyListserver|--/a--| } On-Line Help |--a class="moz-txt-link-freetext" href="http://www.microscopy.com/MicroscopyListserver/FAQ.html"--|http://www.microscopy.com/MicroscopyListserver/FAQ.html|--/a--| } ---------------------------------------------------------------------------- } } Dear ListServers, } } in our lab we have a TEM Jeol JEM-3010 used mainly for HREM studies. } We } are thinking of buying a new chiller, and we'd like to consider } various } brands, not only the current one which is still the one provided by } Jeol } at the installation of the instrument (Neslab). Obviously for HREM we } are concerned with water temperature stability and vibrations. } } What is your experience in this respect? Any advice or comment will be } appreciated! } Thanks } } Davide } } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive.it } } Universita' Ca' Foscari Venezia } Dipartimento di Chimica Fisica } Lab. di Scienza e Tecnologia dei Materiali } Via Torino, 155b } I-30172 Mestre (VE) } Italy } |--/pre--| } |--/blockquote--| } |--pre wrap=""--||--!------| } ==============================Original Headers============================== } 6, 26 -- From |--a class="moz-txt-link-abbreviated" href="mailto:RCsencsits-at-lbl.gov"--|RCsencsits-at-lbl.gov|--/a--| Tue Sep 2 11:04:03 2008 } 6, 26 -- Received: from ironport3.lbl.gov (ironport3.lbl.gov [128.3.41.25]) } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82G43qJ021072 } 6, 26 -- for |--a class="moz-txt-link-rfc2396E" href="mailto:Microscopy-at-microscopy.com"--|<Microscopy-at-microscopy.com>|--/a--|; Tue, 2 Sep 2008 11:04:03 -0500 } 6, 26 -- X-Ironport-SBRS: 2.9 } 6, 26 -- X-IronPort-Anti-Spam-Filtered: true } 6, 26 -- X-IronPort-Anti-Spam-Result: At8CAM4AvUiAAykMgWdsb2JhbACSTgEBECAFmjIJhhwBa30 } 6, 26 -- X-IronPort-AV: E=Sophos;i="4.32,317,1217833200"; } 6, 26 -- d="scan'208";a="4507340" } 6, 26 -- Received: from mta2.lbl.gov ([128.3.41.12]) } 6, 26 -- by ironport3.lbl.gov with ESMTP; 02 Sep 2008 09:04:02 -0700 } 6, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) } 6, 26 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id m82G41SE015983; } 6, 26 -- Tue, 2 Sep 2008 09:04:01 -0700 (PDT) } 6, 26 -- Cc: |--a class="moz-txt-link-abbreviated" href="mailto:Microscopy-at-microscopy.com"--|Microscopy-at-microscopy.com|--/a--| } 6, 26 -- Message-Id: |--a class="moz-txt-link-rfc2396E" href="mailto:9EF31184-F3B3-4F9F-859A-D401429DF162-at-lbl.gov"--|<9EF31184-F3B3-4F9F-859A-D401429DF162-at-lbl.gov>|--/a--| } 6, 26 -- From: Roseann Csencsits |--a class="moz-txt-link-rfc2396E" href="mailto:RCsencsits-at-lbl.gov"--|<RCsencsits-at-lbl.gov>|--/a--| } 6, 26 -- To: |--a class="moz-txt-link-abbreviated" href="mailto:dcristofori-at-unive.it"--|dcristofori-at-unive.it|--/a--| } 6, 26 -- In-Reply-To: |--a class="moz-txt-link-rfc2396E" href="mailto:200809021352.m82DqQ17012174-at-ns.microscopy.com"--|<200809021352.m82DqQ17012174-at-ns.microscopy.com>|--/a--| } 6, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 6, 26 -- Content-Transfer-Encoding: 7bit } 6, 26 -- Mime-Version: 1.0 (Apple Message framework v926) } 6, 26 -- Subject: Re: [Microscopy] chiller for Jeol TEM JEM-3010 } 6, 26 -- Date: Tue, 2 Sep 2008 09:03:59 -0700 } 6, 26 -- References: |--a class="moz-txt-link-rfc2396E" href="mailto:200809021352.m82DqQ17012174-at-ns.microscopy.com"--|<200809021352.m82DqQ17012174-at-ns.microscopy.com>|--/a--| } 6, 26 -- X-Mailer: Apple Mail (2.926) } ==============================End of - Headers============================== } } __________ Information from ESET Smart Security, version of virus signature database 3408 (20080902) __________ } } The message was checked by ESET Smart Security. } } |--a class="moz-txt-link-freetext" href="http://www.eset.com"--|http://www.eset.com|--/a--| } } } } |--/pre--| } |--/blockquote--| } |--br--| } |--/body--| } |--/html--| } } --------------040703090105090808070207-- } } ==============================Original Headers============================== } 44, 38 -- From cell.toli-at-gmail.com Tue Sep 2 12:03:51 2008 } 44, 38 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.240]) } 44, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82H3phJ017036 } 44, 38 -- for |--microscopy-at-microscopy.com--|; 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( [65.190.187.195]) } 44, 38 -- by mx.google.com with ESMTPS id i6sm11655898wxd.2.2008.09.02.10.03.44 } 44, 38 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); } 44, 38 -- Tue, 02 Sep 2008 10:03:45 -0700 (PDT) } 44, 38 -- Message-ID: |--48BD71F1.5050301-at-hillweb.com--| } 44, 38 -- Date: Tue, 02 Sep 2008 13:03:45 -0400 } 44, 38 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) } 44, 38 -- MIME-Version: 1.0 } 44, 38 -- To: microscopy-at-microscopy.com } 44, 38 -- Subject: [Microscopy] Re: chiller for Jeol TEM JEM-3010 } 44, 38 -- References: |--200809021608.m82G8j0a028589-at-ns.microscopy.com--| } 44, 38 -- In-Reply-To: |--200809021608.m82G8j0a028589-at-ns.microscopy.com--| } 44, 38 -- Content-Type: multipart/alternative; } 44, 38 -- boundary="------------040703090105090808070207" } 44, 38 -- From: ANATOLI OLEYNIK |--cell.toli-at-gmail.com--| } ==============================End of - Headers============================== } }
On Sep 2, 2008, at 2:39 AM, nizets2-at-yahoo.com wrote:
} Related to my last question on the list, dealing with the } preparation of frozen samples for TEM, I experienced that 5% water } in acetone, once placed at -80°C, will freeze at the bottom of the } tube. I am very inexperienced in High-Pressure Freezing and Freeze } substitution, but I know that Paul Walther proposed to mix water } with acetone to improve membrane visualization. Well I suppose that } the mix is first cooled down to temperature, so this means that } water separates from acetone in the mix! } Something must be wrong with my argumentation, but I don't see what/ } where. } Can someone please help me?
Dear Stephane, If one looks at a phase diagram of a two-component mixture or solution, one sees that the line separating the liquid and solid phases is at a higher temperature for one of the pure components than for an admixture of the second component, and this is true also for the second component if both components are liquid. (I know that even solid solutes will liquify if the temperature is raised enough, but the statement is true for the water-acetone system.) The liquid-solid line goes to lower temperatures as the amount of the second component increases until it reaches a minimum for a particular ratio of components called the eutectic. If one takes a mixture of the two components and lowers the temperature, the composition of the liquid phase of the mixture is constant until the temperature intersects the liquid-solid line, then if the temperature is lowered further, the component in excess over the eutectic ratio will freeze out of the mixture until the temperature is that at which the eutectic mixture freezes, at which point the remaining liquid is the eutectic mixture. In a diagram with component ratio along the x-axis and temperature along the y-axis, the liquid-solid line generally looks like two concave-downward curves extending from the freezing points of the two components at the lowest and highest fractions of a component (0 and 100%) and intersecting at the eutectic ratio and its freezing point. The starting ratio and temperature define a point in the liquid region. The line that represents what happens drops straight down until it hits the liquid-solid line, then travels along that line until it reaches the eutectic point, then drops straight down again. This line defines the ratio of components in the liquid as the temperature is lowered, then represents the eutectic ratio as the whole mixture freezes. In your case, the 5% water in acetone would seem to be more water than the eutectic, since acetone freezes at -95 C. Did you put the pure acetone-water mixture in a -80 C freezer, or did you first add your specimen? In the latter case, there may have been water in your specimen or some other component may be present, which could complicate the situation. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 7, 23 -- From tivol-at-caltech.edu Tue Sep 2 12:31:34 2008 7, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82HVXPg030871 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 12:31:33 -0500 7, 23 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 7, 23 -- by fire-doxen-postvirus (Postfix) with ESMTP id AA1C22E50C68 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 10:31:33 -0700 (PDT) 7, 23 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 7, 23 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 7, 23 -- by fire-doxen-ssl (Postfix) with ESMTP id 7C5642E50C66 7, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 10:31:32 -0700 (PDT) 7, 23 -- Message-Id: {90C5B836-4558-4C0F-B51D-8652E9422EB0-at-caltech.edu} 7, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- In-Reply-To: {200809020939.m829dgr5012748-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 7, 23 -- Mime-Version: 1.0 (Apple Message framework v926) 7, 23 -- Subject: Re: [Microscopy] water in acetone? 7, 23 -- Date: Tue, 2 Sep 2008 10:31:32 -0700 7, 23 -- References: {200809020939.m829dgr5012748-at-ns.microscopy.com} 7, 23 -- X-Mailer: Apple Mail (2.926) 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m82HVXPg030871 ==============================End of - Headers==============================
The 2008 Fall Meeting of the American Geophysical Union (San Francisco, Dec. 15-19, http://www.agu.org/meetings/fm08/) will include a session entitled "Atmospheric Aerosols and Electron Microscopy".
Session A20 invites studies of an understanding of atmospheric aerosols through the use of electron microscopy and x-ray microanalysis. Studies include but are not limited to microscopic determinations of particle size, morphology, and chemical composition to reveal sources of anthropogenic aerosols vs. natural aerosols and to reveal links between climatic effects of aerosols and the physical and chemical properties of individual particles.
Key Words 0305 Aerosols and Particles 0345 Pollution: Urban and Regional 0394 Instruments and Techniques 1029 Composition of Aerosols and Dust Particles (Geochemistry) 0365 Troposphere: Composition and Chemistry
Abstracts are due without exception by September 10 and acceptances sent Oct. 23-24. Please go to http://submissions3.agu.org/submission/subm-ins.htm for instructions on submitting an abstract. If you wish to submit an abstract and are not a member of AGU, please email Joe or myself. And please forward this email to any interested colleagues.
I hope we'll see you in San Francisco!
Robert Willis, Ph.D. US Environmental Protection Agency National Exposure Research Laboratory! Environmental Characterization and Apportionment Branch MD E205-03 Research Triangle Park, NC 27711 Tel: 919-541-2809 Fax: 919-541-0960 willis.robert-at-epa.gov
Joseph M. Conny, Ph.D.: Surface and Microanalysis Science Division National Institute of Standards and Technology 100 Bureau Drive Stop 8372 Gaithersburg, MD 20899-8372 301-975-3932, fax 301-926-6689 email joseph.conny-at-nist.gov
==============================Original Headers============================== 10, 26 -- From Willis.Robert-at-epamail.epa.gov Tue Sep 2 14:44:58 2008 10, 26 -- Received: from mblast01.rtp.epa.gov (mblast01.rtp.epa.gov [134.67.221.151]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82JiuXq017133 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 14:44:57 -0500 10, 26 -- Received: from mblast01.rtp.epa.gov (localhost.localdomain [127.0.0.1]) 10, 26 -- by localhost (Postfix) with SMTP id EE08F44537 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 15:44:54 -0400 (EDT) 10, 26 -- Received: from mintra01.rtp.epa.gov (mintra01.rtp.epa.gov [134.67.221.153]) 10, 26 -- by mblast01.rtp.epa.gov (Postfix) with ESMTP id E8947443D7 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 15:44:54 -0400 (EDT) 10, 26 -- Received: from mintra01.rtp.epa.gov (localhost.localdomain [127.0.0.1]) 10, 26 -- by localhost (Postfix) with SMTP id DC2CD444F1 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 15:44:54 -0400 (EDT) 10, 26 -- Received: from epahub11.rtp.epa.gov (epahub11.rtp.epa.gov [134.67.222.133]) 10, 26 -- by mintra01.rtp.epa.gov (Postfix) with ESMTP id AF50B444A7 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 15:44:54 -0400 (EDT) 10, 26 -- Subject: Electron Microscopy Session at AGU Fall Meeting 10, 26 -- To: Microscopy-at-microscopy.com 10, 26 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 10, 26 -- Message-ID: {OF00FD0FE1.B77231F9-ON852574B8.006A7938-852574B8.006C7A50-at-epamail.epa.gov} 10, 26 -- From: Willis.Robert-at-epamail.epa.gov 10, 26 -- Date: Tue, 2 Sep 2008 15:44:55 -0400 10, 26 -- X-MIMETrack: Serialize by Router on EPAHUB11/USEPA/US(Release 8.0.1|February 07, 2008) at 10, 26 -- 09/02/2008 03:45:01 PM 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Bruno Humbel and Martin Müller contributed a paper in 1984 at the Budapest meeting regarding acetone and the temperature related uptake of tritiated water. Tritiated water may not have the same hydrogen bonding characteristics as the'normal' isotope and therefore differences may exist. But nevertheless, very useful!
Reference;
Humbel, B and Müller, M Freeze substitution and low-temperature embedding Proc. 8th Eur. Congr. Electron Microscopy, Budapest 1984 (Csanady, A Röhlich, P Szabo, D eds) p 1798-1798
Cheers Jan Leunissen
On 2/09/2008, at 9:39 PM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Dear cryo-colleagues! } Related to my last question on the list, dealing with the } preparation of frozen samples for TEM, I experienced that 5% water } in acetone, once placed at -80°C, will freeze at the bottom of the } tube. I am very inexperienced in High-Pressure Freezing and Freeze } substitution, but I know that Paul Walther proposed to mix water } with acetone to improve membrane visualization. Well I suppose that } the mix is first cooled down to temperature, so this means that } water separates from acetone in the mix! } Something must be wrong with my argumentation, but I don't see what/ } where. } Can someone please help me? } Best regards, } } Stephane } } } } } } ==============================Original } Headers============================== } 5, 21 -- From nizets2-at-yahoo.com Tue Sep 2 04:39:33 2008 } 5, 21 -- Received: from web37408.mail.mud.yahoo.com } (web37408.mail.mud.yahoo.com [209.191.91.140]) } 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m829dX1M012612 } 5, 21 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 04:39:33 } -0500 } 5, 21 -- Received: (qmail 26838 invoked by uid 60001); 2 Sep 2008 } 09:39:33 -0000 } 5, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 5, 21 -- s=s1024; d=yahoo.com; } 5, 21 -- h=X-YMail-OSG:Received:X- } Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content- } Transfer-Encoding:Message-ID; } 5, 21 -- b=V/ } MWD0f02Q4blpZGMfu5wAzNlNFReNU89ZZ2fTDsSFXT2Fahp4xDZXZmjOTSpRy5t1/ } FZPlFvp/CELOHYuXfZF3fm7vzMtT2s } +jokjn2WATJxFZ3DxPedLcM3TOjlx5svXb7i32IeXNZpgnrfTh8nPAQLD1z1v+V } +MWqoQIeHsU=; } 5, 21 -- X-YMail-OSG: } q8JqZjkVM1l.ziUnFAD49PNxWNaLXZQecB697eUnsUUHIElkIsaYfq5US_zXcRShSaAhsE } 31DnxxLqczUxNJFxc_N3woi53eLC2l6Sp6kQ-- } 5, 21 -- Received: from [80.122.101.100] by } web37408.mail.mud.yahoo.com via HTTP; Tue, 02 Sep 2008 02:39:33 PDT } 5, 21 -- X-Mailer: YahooMailRC/1042.48 YahooMailWebService/0.7.218.2 } 5, 21 -- Date: Tue, 2 Sep 2008 02:39:33 -0700 (PDT) } 5, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 5, 21 -- Subject: water in acetone? } 5, 21 -- To: microscopy-at-microscopy.com } 5, 21 -- MIME-Version: 1.0 } 5, 21 -- Content-Type: text/plain; charset=iso-8859-1 } 5, 21 -- Message-ID: {306950.26500.qm-at-web37408.mail.mud.yahoo.com} } 5, 21 -- Content-Transfer-Encoding: 8bit } 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m829dX1M012612 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 11, 20 -- From leunissen-at-aurion.nl Tue Sep 2 17:36:27 2008 11, 20 -- Received: from mta01.xtra.co.nz (mta01.xtra.co.nz [210.54.141.254]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m82MaP3a004645 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 17:36:26 -0500 11, 20 -- Received: from [192.168.1.50] (really [222.152.99.243]) by mta01.xtra.co.nz 11, 20 -- with ESMTP 11, 20 -- id {20080902223623.DAHB7391.mta01.xtra.co.nz-at-[192.168.1.50]} 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 3 Sep 2008 10:36:23 +1200 11, 20 -- Mime-Version: 1.0 (Apple Message framework v753.1) 11, 20 -- In-Reply-To: {200809020939.m829doba012979-at-ns.microscopy.com} 11, 20 -- References: {200809020939.m829doba012979-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 11, 20 -- Message-Id: {72DD14A5-88B4-4897-A7A3-9DE4B1169FD2-at-aurion.nl} 11, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl} 11, 20 -- Subject: Re: [Microscopy] water in acetone? 11, 20 -- Date: Wed, 3 Sep 2008 10:36:22 +1200 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- X-Mailer: Apple Mail (2.753.1) 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m82MaP3a004645 ==============================End of - Headers==============================
I would just like to say thanks for all the helpful replies, and special thanks to David Hull who sent me the manual for this specific part. It pointed out the single glaring reason why my efforts to construct a similar device had failed to produce a rewarding purple glow; I neglected to "set the desired glow discharge current with the potentiometer located on the MED010." i.e. I forgot to turn a knob. I knew it was a simple answer!
Anyway, thanks again, and we now have a functioning carbon evaporator/glow discharger and can finally get rid of that old hulk of a machine that's taking up precious space.
-Brad
-----Original Message-----
} Date: Mon Aug 18 09:52:39 PDT 2008 } From: "Bradford Ross" {bnross-at-interchange.ubc.ca} } Subject: Balzers MED 010 Glow Discharge Device } To: Microscopy-at-microscopy.com } } Hello Everyone, } } We have an old Balzer's MED 010 coating system, and I am trying to either find the original glow discharge attachment that fits in the vacuum chamber, or a detailed picture and/or schematic of this part so that I can have our machine shop guy make something similar. The Balzer's part number is BU 007 284 -T if that helps. } } If anyone knows where I could find this part, or if you can tell me a little more about how it works so I can make something similar, that would be a huge help! Our even older coating system which we use for glow discharging has blown a turbo pump and we would just like to get rid of it and use the MED for carbon coating/glow discharging. } } Thanks, } -- } Bradford Ross } } Microscopy Technician } BioImaging Facility } University of British Columbia } 6270 University Blvd. } Vancouver, B.C. } Canada } V6T 1Z4 } } phone 604-822-6996 -- Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
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Title-Subject: [Filtered] Increase contrast in TEM
Question: Hi everyone,
I'm having a trouble clearly visualizing synaptic vesicles from rat brain staining. I need to count synaptic vesicles so it is critical to get clear staining. If anyone can share any ideas on increasing contrast on EM images, I would appreciate it.
1) Sample: rat brain, fixed with 4% PFA 0.5% glutaraldehyde, section vibrosliced 2) Staining: nanogold, HQ silver, 1% Osmium 30 min in Sodium Cacodylate buffer+ 0.13 M sucrose 3) aqueous Uranyl staining 5 min Reynold's lead 8 min
We experienced precpitates when increased Osmium incubation time, so we are limited to 30 min incubation time. We are trying now to vary Uranyl and lead staining time but it doesn't seem to make much difference.
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Email: jdaues-at-gmail.com Name: jdaues
Title-Subject: [Filtered] micropscope for amatuer mycology
Question: I am thinking of getting a microscope for observing fungus spores. I'd like something that would connect via USB to a computer
Here's information from a mycology forum:
Fungal spores are generally in the range of 10 - 100 micrometres and the detail on the spore wall which you will want to see in many cases is going to be of the order of 1 micrometre. For these sizes you will need a magnification of x 400 or ideally x1000. You will not get a good new microscope with x 1000 magnification cheaply. You will get some inexpensive good mikes with x 400 magnification and some with USB connectivity.
Not all contrast problems can, or need to be solved by changing the stain. It's a bit of an old solution, but try using a smaller Objective Aperture - better contrast, better resolution - not that the size of OA is as important for resolution with thin sections as it is with negative staining. You could also change your acceleration voltage.
Paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 23 -- From paul_hazelton-at-umanitoba.ca Tue Sep 2 23:06:45 2008 5, 23 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8346jOR006771 5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 23:06:45 -0500 5, 23 -- Received: from [192.168.100.102] (wnpgmb014rw-ad01-9-208.dynamic.mts.net [207.161.9.208]) 5, 23 -- (authenticated bits=0) 5, 23 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m8346dgG025063 5, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 23 -- Tue, 2 Sep 2008 23:06:43 -0500 (CDT) 5, 23 -- Message-ID: {48BE0D52.8020603-at-umanitoba.ca} 5, 23 -- Date: Tue, 02 Sep 2008 23:06:42 -0500 5, 23 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 23 -- Organization: University of Manitoba 5, 23 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 5, 23 -- MIME-Version: 1.0 5, 23 -- To: cychung-at-mclean.harvard.edu 5, 23 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 5, 23 -- Subject: Re: [Microscopy] viaWWW: Increase contrast in TEM 5, 23 -- References: {200809030032.m830WYd2007763-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {200809030032.m830WYd2007763-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit 5, 23 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
One comment about the precipitates: you may be able to get this under control by using 2-mercaptoethanol in your processing steps. If you're interested, you can find protocols and formulas on our website at http://www.emc.missouri.edu/Pdfs/General%202-ME%20Microwave%20Processing %20Protocol.pdf and http://www.emc.missouri.edu/Pdfs/Common%20Buffer%20and%20Fixatives%20for %20Electron%20Microscopy.pdf. We have never done it in a procedure exactly like you describe, but it may be worth a try.
Also, you can try "double staining", which involves starting with 2-3 minutes of lead stain, followed by uranyl acetate, and ending with a second lead stain for 8-10 minutes. This can provide a dramatic increase in specimen contrast.
Best of luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: cychung-at-mclean.harvard.edu [mailto:cychung-at-mclean.harvard.edu] Sent: Tuesday, September 02, 2008 7:32 PM To: Tindall, Randy D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both cychung-at-mclean.harvard.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] Increase contrast in TEM
Question: Hi everyone,
I'm having a trouble clearly visualizing synaptic vesicles from rat brain staining. I need to count synaptic vesicles so it is critical to get clear staining. If anyone can share any ideas on increasing contrast on EM images, I would appreciate it.
1) Sample: rat brain, fixed with 4% PFA 0.5% glutaraldehyde, section vibrosliced 2) Staining: nanogold, HQ silver, 1% Osmium 30 min in Sodium Cacodylate buffer+ 0.13 M sucrose 3) aqueous Uranyl staining 5 min Reynold's lead 8 min
We experienced precpitates when increased Osmium incubation time, so we are limited to 30 min incubation time. We are trying now to vary Uranyl and lead staining time but it doesn't seem to make much difference.
In addition to the excellent ideas already offered (smaller objective aperature, reduced accelerating voltage, what we used to call PUP stain, lead uranyl lead)
Cut thicker sections. More tissue will pick up more stain and give more contrast.
Geoff
cychung-at-mclean.harvard.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both cychung-at-mclean.harvard.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: cychung-at-mclean.harvard.edu } Name: Chee Yeun } } Organization: Harvard Medical School } } Title-Subject: [Filtered] Increase contrast in TEM } } Question: Hi everyone, } } I'm having a trouble clearly visualizing synaptic vesicles from rat } brain staining. I need to count synaptic vesicles so it is critical } to get clear staining. If anyone can share any ideas on increasing } contrast on EM images, I would appreciate it. } } 1) Sample: rat brain, fixed with 4% PFA 0.5% glutaraldehyde, section } vibrosliced } 2) Staining: nanogold, HQ silver, 1% Osmium 30 min in Sodium } Cacodylate buffer+ 0.13 M sucrose } 3) aqueous Uranyl staining 5 min Reynold's lead 8 min } } We experienced precpitates when increased Osmium incubation time, so } we are limited to 30 min incubation time. We are trying now to vary } Uranyl and lead staining time but it doesn't seem to make much } difference. } } Thank you. } } Chee-Yeun } } Login Host: 71.184.189.60 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Tue Sep 2 19:31:14 2008 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m830VDYW004249 } 11, 11 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 19:31:14 -0500 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240800c4e38b3971c5-at-[206.69.208.22]} } 11, 11 -- Date: Tue, 2 Sep 2008 19:31:12 -0500 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: cychung-at-mclean.harvard.edu (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: Increase contrast in TEM } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 28 -- From mcauliff-at-umdnj.edu Wed Sep 3 09:33:30 2008 8, 28 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m83EXUCi004442 8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 3 Sep 2008 09:33:30 -0500 8, 28 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 8, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E5C07A7B7A 8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 3 Sep 2008 10:33:29 -0400 (EDT) 8, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 8, 28 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 2A569A7B5E 8, 28 -- for {microscopy-at-microscopy.com} ; Wed, 3 Sep 2008 10:33:29 -0400 (EDT) 8, 28 -- Received: from ([10.32.15.167]) 8, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.160324331; 8, 28 -- Wed, 03 Sep 2008 10:33:09 -0400 8, 28 -- MIME-version: 1.0 8, 28 -- Content-transfer-encoding: 7BIT 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 8, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 8, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0K6M004GKJ389970-at-umduwc01.umdnj.edu} for 8, 28 -- microscopy-at-microscopy.com; Wed, 03 Sep 2008 10:33:09 -0400 (EDT) 8, 28 -- Message-id: {48BEA05A.5020507-at-umdnj.edu} 8, 28 -- Date: Wed, 03 Sep 2008 10:34:02 -0400 8, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 28 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 8, 28 -- To: cychung-at-mclean.harvard.edu, microscopy-at-microscopy.com 8, 28 -- Subject: Re: [Microscopy] viaWWW: Increase contrast in TEM 8, 28 -- References: {200809030031.m830VmMV005727-at-ns.microscopy.com} 8, 28 -- In-reply-to: {200809030031.m830VmMV005727-at-ns.microscopy.com} ==============================End of - Headers==============================
Can anyone recommend a good online resource for rebuilding a rotary vane vac pump? (Varian or other). I ordered a rebuild kit that I hoped would have at least cursory instructions but none were included.
TIA, Jay
Jay Campbell University of Wisconsin Dept of Biochemistry, EM facility
Chee Yuen, I have used protocols similar to yours but usually included one hour in 2% uranyl acetate just prior to dehydration. My epoxy is an Epon equivalent. I cut pale gold sections and use a support film. I stain with 2% acqueous uranyl acetate for one hour and then for twenty minutes in Reynolds lead citrate (this stain will not perform after several months of storage--try some freshly made stain). I typically use 80kV and a medium size objective aperture. Synapses are very apparent when the primary fixative perfusion is good. The suggestions by other Listers are also appropriate. Larry
mcauliff-at-umdnj.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } In addition to the excellent ideas already offered (smaller objective } aperature, reduced accelerating voltage, what we used to call PUP stain, } lead uranyl lead) } } Cut thicker sections. More tissue will pick up more stain and give more } contrast. } } Geoff } } cychung-at-mclean.harvard.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both cychung-at-mclean.harvard.edu as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: cychung-at-mclean.harvard.edu } } Name: Chee Yeun } } } } Organization: Harvard Medical School } } } } Title-Subject: [Filtered] Increase contrast in TEM } } } } Question: Hi everyone, } } } } I'm having a trouble clearly visualizing synaptic vesicles from rat } } brain staining. I need to count synaptic vesicles so it is critical } } to get clear staining. If anyone can share any ideas on increasing } } contrast on EM images, I would appreciate it. } } } } 1) Sample: rat brain, fixed with 4% PFA 0.5% glutaraldehyde, section } } vibrosliced } } 2) Staining: nanogold, HQ silver, 1% Osmium 30 min in Sodium } } Cacodylate buffer+ 0.13 M sucrose } } 3) aqueous Uranyl staining 5 min Reynold's lead 8 min } } } } We experienced precpitates when increased Osmium incubation time, so } } we are limited to 30 min incubation time. We are trying now to vary } } Uranyl and lead staining time but it doesn't seem to make much } } difference. } } } } Thank you. } } } } Chee-Yeun } } } } Login Host: 71.184.189.60 } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 11, 11 -- From zaluzec-at-microscopy.com Tue Sep 2 19:31:14 2008 } } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m830VDYW004249 } } 11, 11 -- for {microscopy-at-microscopy.com} ; Tue, 2 Sep 2008 19:31:14 -0500 } } 11, 11 -- Mime-Version: 1.0 } } 11, 11 -- Message-Id: {p06240800c4e38b3971c5-at-[206.69.208.22]} } } 11, 11 -- Date: Tue, 2 Sep 2008 19:31:12 -0500 } } 11, 11 -- To: microscopy-at-microscopy.com } } 11, 11 -- From: cychung-at-mclean.harvard.edu (by way of MicroscopyListserver) } } 11, 11 -- Subject: viaWWW: Increase contrast in TEM } } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } ==============================End of - Headers============================== } } } } } } } }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both grb-at-ufl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: grb-at-ufl.edu Name: Gerald Bourne
Organization: University of Florida, Major Analytical Instrumentation Center
Question: Hi, I am the system administrator for a DB235 Strata Dual Beam FEG FIB. Our microscope is controlled by XP 2.29. In the past, we have left a generic login on the scope and allowed users to anonymously login. However, I now assign an individual password to each user.
I cannot seem to generate user logs by using the Logging Report utility. When I click on the application, it opens just like the manual says, but when I click 'create', no reports are generated, and the report complete message does not appear. I have attempted to copy the database c:\xp\acctlog\userlog.mdb and view it on a computer that has MSAccess, but I get a message stating the database is locked. Although I am not sure if this is related, when I create a new user in XP 2.29 I get a message saying unrecognized database, but the new user is indeed added.
All I really want to do is generate userlogs from the microscope. Any suggestions?
Are you logged in as Administrator when you try this?
gary g.
At 04:50 PM 9/3/2008, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
IMO, you will never get satisfaction with WinNT. Change to Win2K or at best, WinXP Pro. Everything changes.
NT is dead...IMO.
FEI seems to have difficulty moving to the newer OS options. I've seen some stuck at Win2K. That is way better than NT.
Good luck.
gary g.
At 06:43 PM 9/3/2008, you wrote:
} Gary } } I am logged in on xP 2.29 as the administrator. The computer runs } Win NT and there is no specific log in for it, so I imagine it is an } admin login with no password. } } Are you logged in as Administrator when you try this? } } } } gary g.
} Question: Hi, I am the system administrator for a DB235 Strata Dual } Beam FEG FIB. Our microscope is controlled by XP 2.29. In the past, } we have left a generic login on the scope and allowed users to } anonymously login. However, I now assign an individual password to } each user. } } I cannot seem to generate user logs by using the Logging Report } utility. When I click on the application, it opens just like the } manual says, but when I click 'create', no reports are generated, and } the report complete message does not appear. I have attempted to } copy the database c:\xp\acctlog\userlog.mdb and view it on a computer } that has MSAccess, but I get a message stating the database is } locked. Although I am not sure if this is related, when I create a } new user in XP 2.29 I get a message saying unrecognized database, but } the new user is indeed added. } } All I really want to do is generate userlogs from the microscope. } Any suggestions?
We have written a simple logging program in Python for our Hitachi 3000 Walkup-SEM. Instead of clicking on the normal start icon,they click on our wrapper icon. This records their user-ID, the date, and hours used to a simple comma separated file. At the end of the quarter, the admin can open this file in Excel, sort by userID and generate a report. The whole reporting piece would be easy to implement in either VBA or Python, it just hasn't made it to my radar screen yet. One could also enter the time directly into a data base with SQL wrappers available in Python. We didn't do that because we cannot have NT machines directly on our corporate network any more.
==============================Original Headers============================== 3, 20 -- From jrminter-at-rochester.rr.com Thu Sep 4 06:41:51 2008 3, 20 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.124]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m84BfpLm004527 3, 20 -- for {Microscopy-at-Microscopy.com} ; Thu, 4 Sep 2008 06:41:51 -0500 3, 20 -- Received: from hrndva-web21-z02 ([10.128.132.172]) 3, 20 -- by hrndva-smta02.mail.rr.com with ESMTP 3, 20 -- id {20080904114151.ZSHS15198.hrndva-smta02.mail.rr.com-at-hrndva-web21-z02} 3, 20 -- for {Microscopy-at-Microscopy.com} ; Thu, 4 Sep 2008 11:41:51 +0000 3, 20 -- Message-ID: {19377132.1413781220528511413.JavaMail.root-at-hrndva-web21-z02} 3, 20 -- Date: Thu, 4 Sep 2008 7:41:51 -0400 3, 20 -- From: {jrminter-at-rochester.rr.com} 3, 20 -- To: Microscopy-at-Microscopy.com 3, 20 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: FEI DB235 3, 20 -- logging report 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; charset=utf-8 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Priority: 3 (Normal) 3, 20 -- Sensitivity: Normal 3, 20 -- X-Originating-IP: from 67.240.238.57 by webmail.rochester.rr.com; Thu, 4 Sep 2008 11:41:50 +0000 ==============================End of - Headers==============================
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Wednesday, September 03, 2008 11:13 PM To: lherault-at-bu.edu
IMO, you will never get satisfaction with WinNT. Change to Win2K or at best, WinXP Pro. Everything changes.
NT is dead...IMO.
FEI seems to have difficulty moving to the newer OS options. I've seen some stuck at Win2K. That is way better than NT.
Good luck.
gary g.
At 06:43 PM 9/3/2008, you wrote:
} Gary } } I am logged in on xP 2.29 as the administrator. The computer runs } Win NT and there is no specific log in for it, so I imagine it is an } admin login with no password. } } Are you logged in as Administrator when you try this? } } } } gary g.
} IMO, you will never get satisfaction with WinNT. Change } to Win2K or at best, WinXP Pro. Everything changes. } } NT is dead...IMO. } } FEI seems to have difficulty moving to the newer } OS options. I've seen some stuck at Win2K. That } is way better than NT.
Well, the only real big difference between WinNT and 2K/XP is USB-support, if I am not mistaken... The next big step is 64bit, as in XP64, but the downward compatibility is not really enjoyable..!
;-) Torsten
==============================Original Headers============================== 13, 28 -- From Torsten.Fregin-at-zoologie.uni-hamburg.de Thu Sep 4 08:42:28 2008 13, 28 -- Received: from mailhost.uni-hamburg.de (mailhost.uni-hamburg.de [134.100.32.155]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m84DgSeo003469 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 08:42:28 -0500 13, 28 -- Received: from localhost (localhost [127.0.0.1]) 13, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 8A7AF100B14 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 15:42:27 +0200 (CEST) 13, 28 -- X-Virus-Scanned: by University of Hamburg (RRZ/mailhost) 13, 28 -- Received: from mailhost.uni-hamburg.de ([127.0.0.1]) 13, 28 -- by localhost (mailhost.uni-hamburg.de [127.0.0.1]) (amavisd-new, port 10024) 13, 28 -- with LMTP id 4DrIAILD2fBe for {microscopy-at-microscopy.com} ; 13, 28 -- Thu, 4 Sep 2008 15:42:27 +0200 (CEST) 13, 28 -- Received: from [134.100.200.6] (TorstenF.zoologie.uni-hamburg.de [134.100.200.6]) 13, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 69A67100899 13, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 15:42:27 +0200 (CEST) 13, 28 -- From: Torsten.Fregin-at-zoologie.uni-hamburg.de 13, 28 -- To: microscopy-at-microscopy.com 13, 28 -- Date: Thu, 04 Sep 2008 15:43:04 +0200 13, 28 -- MIME-Version: 1.0 13, 28 -- Subject: Re: [Microscopy] Re: [Filtered] MicroscopyListserverviaWWW: FEI 13, 28 -- Message-ID: {48C00208.6003.B9B801-at-Torsten.Fregin.zoologie.uni-hamburg.de} 13, 28 -- Priority: normal 13, 28 -- In-reply-to: {200809040312.m843COZM005906-at-ns.microscopy.com} 13, 28 -- References: {200809040312.m843COZM005906-at-ns.microscopy.com} 13, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 13, 28 -- Content-type: text/plain; charset=US-ASCII 13, 28 -- Content-transfer-encoding: 7BIT 13, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
Make sure to input the proper "path" in the logon and logoff files, which is determined by the location of your file. The created userlog file is a text file which you can read with any text editing program. Make sure the userlog file is located in a directory, where users who do not have administrative access will have access to.
Krassimir.
========================= Krassimir N. Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California Riverside, CA 92521
tel:951 827 2998 fax: 951 827 2489
bozhilov-at-ucr.edu ========================
On Sep 4, 2008, at 4:49 AM, jrminter-at-rochester.rr.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } } Question: Hi, I am the system administrator for a DB235 Strata Dual } } Beam FEG FIB. Our microscope is controlled by XP 2.29. In the past, } } we have left a generic login on the scope and allowed users to } } anonymously login. However, I now assign an individual password to } } each user. } } } } I cannot seem to generate user logs by using the Logging Report } } utility. When I click on the application, it opens just like the } } manual says, but when I click 'create', no reports are generated, and } } the report complete message does not appear. I have attempted to } } copy the database c:\xp\acctlog\userlog.mdb and view it on a computer } } that has MSAccess, but I get a message stating the database is } } locked. Although I am not sure if this is related, when I create a } } new user in XP 2.29 I get a message saying unrecognized database, but } } the new user is indeed added. } } } } All I really want to do is generate userlogs from the microscope. } } Any suggestions? } } We have written a simple logging program in Python for our Hitachi } 3000 Walkup-SEM. Instead of clicking on the normal start icon,they } click on our wrapper icon. This records their user-ID, the date, } and hours used to a simple comma separated file. At the end of the } quarter, the admin can open this file in Excel, sort by userID and } generate a report. The whole reporting piece would be easy to } implement in either VBA or Python, it just hasn't made it to my } radar screen yet. One could also enter the time directly into a } data base with SQL wrappers available in Python. We didn't do that } because we cannot have NT machines directly on our corporate } network any more. } } ==============================Original } Headers============================== } 3, 20 -- From jrminter-at-rochester.rr.com Thu Sep 4 06:41:51 2008 } 3, 20 -- Received: from hrndva-omtalb.mail.rr.com (hrndva- } omtalb.mail.rr.com [71.74.56.124]) } 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m84BfpLm004527 } 3, 20 -- for {Microscopy-at-Microscopy.com} ; Thu, 4 Sep 2008 06:41:51 } -0500 } 3, 20 -- Received: from hrndva-web21-z02 ([10.128.132.172]) } 3, 20 -- by hrndva-smta02.mail.rr.com with ESMTP } 3, 20 -- id {20080904114151.ZSHS15198.hrndva- } smta02.mail.rr.com-at-hrndva-web21-z02} } 3, 20 -- for {Microscopy-at-Microscopy.com} ; Thu, 4 Sep 2008 } 11:41:51 +0000 } 3, 20 -- Message-ID: } {19377132.1413781220528511413.JavaMail.root-at-hrndva-web21-z02} } 3, 20 -- Date: Thu, 4 Sep 2008 7:41:51 -0400 } 3, 20 -- From: {jrminter-at-rochester.rr.com} } 3, 20 -- To: Microscopy-at-Microscopy.com } 3, 20 -- Subject: Re: [Microscopy] [Filtered] } MicroscopyListserverviaWWW: FEI DB235 } 3, 20 -- logging report } 3, 20 -- MIME-Version: 1.0 } 3, 20 -- Content-Type: text/plain; charset=utf-8 } 3, 20 -- Content-Transfer-Encoding: 7bit } 3, 20 -- X-Priority: 3 (Normal) } 3, 20 -- Sensitivity: Normal } 3, 20 -- X-Originating-IP: from 67.240.238.57 by } webmail.rochester.rr.com; Thu, 4 Sep 2008 11:41:50 +0000 } ==============================End of - } Headers==============================
Our Leica EMPact high-pressure freezer is ailing. It suddenly began dumping vast quantities of hydraulic fluid out through the specimen loading port, which is not a good thing. Upon locking the specimen into the freezing chamber, it is possible to watch the hydraulic fluid level drop in the sight glass on top of the machine.
My question is: has anyone had something like this happen and been able to fix it in-house? It may be something as simple as a failed seal or o-ring that could be easily replaced, but I can't tell without ripping into the machine.
Due to its infrequent use by our clients, we don't have a service contract on this machine, and we have a hard time justifying the $5000 minimum P.O. it takes to open a service ticket. My request for a brief phone chat with a Leica service engineer to ask some questions was met with, well, icy indifference.
Any advice would be helpful on this one. It would be a shame to park this equipment in a corner and let it rust.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 8, 25 -- From TindallR-at-missouri.edu Thu Sep 4 11:40:58 2008 8, 25 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m84Geu1q005860 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 11:40:57 -0500 8, 25 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 25 -- Thu, 4 Sep 2008 11:40:54 -0500 8, 25 -- x-mimeole: Produced By Microsoft Exchange V6.5 8, 25 -- Content-class: urn:content-classes:message 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="us-ascii" 8, 25 -- Subject: Leica EMPact High Pressure Freezer problem 8, 25 -- Date: Thu, 4 Sep 2008 11:40:54 -0500 8, 25 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7918-at-UM-XMAIL08.um.umsystem.edu} 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- Thread-Topic: Leica EMPact High Pressure Freezer problem 8, 25 -- Thread-Index: AckOrP+CiegtAE0CRLmXbsQFuQa56g== 8, 25 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 8, 25 -- To: {microscopy-at-microscopy.com} 8, 25 -- Cc: "Katz, Martin" {KatzM-at-health.missouri.edu} , 8, 25 -- "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 8, 25 -- X-OriginalArrivalTime: 04 Sep 2008 16:40:54.0736 (UTC) FILETIME=[FFE87D00:01C90EAC] 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m84Geu1q005860 ==============================End of - Headers==============================
I have been following the thread on computer platforms with some interest. While I appreciate the fact that there are a number of computer experts out there, please understand a microscope has a control system, which is tested extensively. It is then sold as a functioning package. Part of the ease of operation of these systems, is to make them look like the desktop PC systems available at that time. This has some consequences, mostly good but some not so good.
A lot of systems have computers, a CM12 or CM20 runs an 8086 microprocessor, your car also has a computer; yet there has never been a request to upgrade one of those to XP or WIN2000, and the argument is never made that it is a 'minimum requirement' for these to run on XP or WIN2K to function...
There is also the aspect to take into account that the hardware changes at a given rate, but computer platforms change at a much higher rate. It is very difficult to be able to keep up with both. Please consider this: How much testing is required to be able to do a 'simple' upgrade from WIN NT to WIN 2K on a platform which was sold in 1993, is configured with numerous accessories (some/most of which not built by FEI, with hardware acquisition cards that do not have drivers for later operating systems) combined with the shear variations in equipment configurations sold? Who would pay for all of this testing? Where can we test it?
As an example, (and this is why I am writing this) just looking at S/TEM systems. There are 120kV, 200kV and 300kV systems. Then there are 4 different lens configurations, not all are offered on all accelerating voltages, then there are around 5 digital camera systems possible, and at least three different EDS vendors. Then there are scanning attachments, BF/DF detectors or HAADF detectors, EELS spectrometers, Lorentz lenses and also a choice between field emission guns or thermionic emitters. This is BEFORE we even look at all the 'special requests' we get on a daily basis for unique research projects that are important to YOU, the end user.
We build the system you require, and we test as much as we can that all the options work, intereract, and perform to the standards required on the system before it is shipped. Does anybody want to hazard a guess how many TEM systems in the field sold by FEI are identical? There have been over 1000 Tecnai's sold, and there are over 700 different configurations. As a software programmer, considering upgrading all of these unique configurations is enough to give anyone a headache. THIS is why a software upgrade is a very major undertaking, and requires a huge resource level. This is also why these upgrades are typically priced high, because of unplanned 'eventualities'; where an experienced SW engineer has to travel to a customer site in order to make an old accessory work with a new operating system.
Look at it from another point of view. If it works, and it works to the level it was designed for, don't try to upgrade it. If your car is running well, you don't change the computer do you?
We try to make all systems upgradeable for as long as we can, but sometimes it just does not make economic sense to upgrade a system anymore, and it should be used until no longer serviceable; which typically is many, many years after purchase.
I am an employee of FEI company, the views expressed in this post are my own, and I am dedicated to make all S/TEM systems sold by FEI work at the level they were designed to. Truth be told, I have performed some software upgrades when required to...
Jan
Dr. Jan Ringnalda S/TEM Product Marketing Engineer
* FEI Company 473 McGuigg, 2041 College Rd, Columbus, OH 43210 USA * Tel. : +1 614 292 2493 * Fax. : +1 614 292 7523 * Mobile : +1 614 284 7946 * E-mail : jringnalda-at-fei.com URL : http://www.fei.com
==============================Original Headers============================== 16, 28 -- From Jan.Ringnalda-at-fei.com Thu Sep 4 13:08:35 2008 16, 28 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m84I8Yb8023492 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 13:08:34 -0500 16, 28 -- X-WSS-ID: 0K6ONQ8-01-KFK-01 16, 28 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 16, 28 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 26CDD94D6E7 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 11:08:31 -0700 (PDT) 16, 28 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 16, 28 -- Thu, 4 Sep 2008 11:08:29 -0700 16, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 28 -- Content-class: urn:content-classes:message 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Type: text/plain; 16, 28 -- charset="us-ascii" 16, 28 -- Subject: RE: FEI software 16, 28 -- Date: Thu, 4 Sep 2008 11:08:23 -0700 16, 28 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB813C0A83F-at-hlexc03.w2k.feico.com} 16, 28 -- X-MS-Has-Attach: 16, 28 -- X-MS-TNEF-Correlator: 16, 28 -- Thread-Topic: FEI software 16, 28 -- Thread-Index: AckOjamMp9GW/VGDRqqDt1eFp6D0ewAAtbqgAAoq8eA= 16, 28 -- References: {200809041256.m84CuT7V030230-at-ns.microscopy.com} 16, 28 -- From: "Ringnalda, Jan" {Jan.Ringnalda-at-fei.com} 16, 28 -- To: {microscopy-at-microscopy.com} 16, 28 -- X-OriginalArrivalTime: 04 Sep 2008 18:08:29.0572 (UTC) FILETIME=[3C08D440:01C90EB9] 16, 28 -- Content-Transfer-Encoding: 8bit 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m84I8Yb8023492 ==============================End of - Headers==============================
On Sep 4, 2008, at 11:08 AM, Jan.Ringnalda-at-fei.com wrote:
} Look at it from another point of view. If it works, and it } works to the level it was designed for, don't try to upgrade it. If } your car is running well, you don't change the computer do you?
Dear Jan, I do sympathize with your point of view, and I have been burned by upgrades so often that I have come to dread each time when someone "improves the system". One small case in point is that in earlier versions of FEI's Tecnai software there was an option to reestablish the HT after the cryo cycle was completed. This is a good idea, since leaving the HT on as much as possible leads to better stability. Unfortunately, the last few versions of the software no longer have this feature. Since this feature had been available and, therefore, presumably tested, how much of an effort would it be to reinstate it on the newer versions? Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Sep 4 13:54:43 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m84IshUb005822 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 13:54:43 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 58CA966E0137 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 11:54:43 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 063C266E0A09 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 11:54:41 -0700 (PDT) 6, 22 -- Message-Id: {A5869A6F-0BAE-444A-9661-AF847B965129-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200809041808.m84I8m7n023625-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v926) 6, 22 -- Subject: Re: [Microscopy] RE: FEI software 6, 22 -- Date: Thu, 4 Sep 2008 11:54:41 -0700 6, 22 -- References: {200809041808.m84I8m7n023625-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.926) ==============================End of - Headers==============================
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Question: We have a Gatan Model 623 Disc Grinder which does not appear to be advancing the sample correctly. Polishing seems to be going along smoothly and then all of a sudden with a turn of 5 microns or less the sample catches and is frequently pulled off the specimen mount and/or severely damaged. If the sample remains mounted, it is now sitting 10 to 100+ microns above the plane of the disc grinder! This seems to occur regardless of the grit of the paper, the RPMs of the polisher, and the sample material. We have taken the disc grinder apart and the threads do not look damaged. We have also replaced the o-ring and the spring. Any ideas on what might be going on and/or how to solve this?
Thanks,
Michelle Ritorto Cavaliere, Ph.D. Senior Research Scientist MVA Scientific Consultants, Inc. 3300 Breckinridge Blvd., Suite 400 Duluth, GA 30096 Phone: 770-662-8509 Fax: 770-662-8532
Your exaustive list is pretty impressive, but as a biologist, I don't see how the fact of having various HT or lense configurations can have such an important impact on the software programming and compatibility. Are there critical changes between of software that drives a 120kV microscope and a 300kV microscope? In any case, I am pretty happy of the last software upgrade of our FEI (I am not talking about OS here), which really improved the stability. As for the cryo-cycle, if Bill was talking about the cryo-cycle after using the cold finger, on our FEI G20 the HT is not affected by the cryo-cycle. I usually start it in the evening and the microscope is ready the next morning!
Regards, Stephane
----- Original Message ---- X-from: "Jan.Ringnalda-at-fei.com" {Jan.Ringnalda-at-fei.com} To: nizets2-at-yahoo.com Sent: Thursday, September 4, 2008 8:13:31 PM
Dear All,
I have been following the thread on computer platforms with some interest. While I appreciate the fact that there are a number of computer experts out there, please understand a microscope has a control system, which is tested extensively. It is then sold as a functioning package. Part of the ease of operation of these systems, is to make them look like the desktop PC systems available at that time. This has some consequences, mostly good but some not so good.
A lot of systems have computers, a CM12 or CM20 runs an 8086 microprocessor, your car also has a computer; yet there has never been a request to upgrade one of those to XP or WIN2000, and the argument is never made that it is a 'minimum requirement' for these to run on XP or WIN2K to function...
There is also the aspect to take into account that the hardware changes at a given rate, but computer platforms change at a much higher rate. It is very difficult to be able to keep up with both. Please consider this: How much testing is required to be able to do a 'simple' upgrade from WIN NT to WIN 2K on a platform which was sold in 1993, is configured with numerous accessories (some/most of which not built by FEI, with hardware acquisition cards that do not have drivers for later operating systems) combined with the shear variations in equipment configurations sold? Who would pay for all of this testing? Where can we test it?
As an example, (and this is why I am writing this) just looking at S/TEM systems. There are 120kV, 200kV and 300kV systems. Then there are 4 different lens configurations, not all are offered on all accelerating voltages, then there are around 5 digital camera systems possible, and at least three different EDS vendors. Then there are scanning attachments, BF/DF detectors or HAADF detectors, EELS spectrometers, Lorentz lenses and also a choice between field emission guns or thermionic emitters. This is BEFORE we even look at all the 'special requests' we get on a daily basis for unique research projects that are important to YOU, the end user.
We build the system you require, and we test as much as we can that all the options work, intereract, and perform to the standards required on the system before it is shipped. Does anybody want to hazard a guess how many TEM systems in the field sold by FEI are identical? There have been over 1000 Tecnai's sold, and there are over 700 different configurations. As a software programmer, considering upgrading all of these unique configurations is enough to give anyone a headache. THIS is why a software upgrade is a very major undertaking, and requires a huge resource level. This is also why these upgrades are typically priced high, because of unplanned 'eventualities'; where an experienced SW engineer has to travel to a customer site in order to make an old accessory work with a new operating system.
Look at it from another point of view. If it works, and it works to the level it was designed for, don't try to upgrade it. If your car is running well, you don't change the computer do you?
We try to make all systems upgradeable for as long as we can, but sometimes it just does not make economic sense to upgrade a system anymore, and it should be used until no longer serviceable; which typically is many, many years after purchase.
I am an employee of FEI company, the views expressed in this post are my own, and I am dedicated to make all S/TEM systems sold by FEI work at the level they were designed to. Truth be told, I have performed some software upgrades when required to...
Jan
Dr. Jan Ringnalda S/TEM Product Marketing Engineer
* FEI Company 473 McGuigg, 2041 College Rd, Columbus, OH 43210 USA * Tel. : +1 614 292 2493 * Fax. : +1 614 292 7523 * Mobile : +1 614 284 7946 * E-mail : jringnalda-at-fei.com URL : http://www.fei.com
==============================Original Headers============================== 16, 28 -- From Jan.Ringnalda-at-fei.com Thu Sep 4 13:08:35 2008 16, 28 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m84I8Yb8023492 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 13:08:34 -0500 16, 28 -- X-WSS-ID: 0K6ONQ8-01-KFK-01 16, 28 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 16, 28 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 26CDD94D6E7 16, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Sep 2008 11:08:31 -0700 (PDT) 16, 28 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 16, 28 -- Thu, 4 Sep 2008 11:08:29 -0700 16, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 28 -- Content-class: urn:content-classes:message 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Type: text/plain; 16, 28 -- charset="us-ascii" 16, 28 -- Subject: RE: FEI software 16, 28 -- Date: Thu, 4 Sep 2008 11:08:23 -0700 16, 28 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB813C0A83F-at-hlexc03.w2k.feico.com} 16, 28 -- X-MS-Has-Attach: 16, 28 -- X-MS-TNEF-Correlator: 16, 28 -- Thread-Topic: FEI software 16, 28 -- Thread-Index: AckOjamMp9GW/VGDRqqDt1eFp6D0ewAAtbqgAAoq8eA= 16, 28 -- References: {200809041256.m84CuT7V030230-at-ns.microscopy.com} 16, 28 -- From: "Ringnalda, Jan" {Jan.Ringnalda-at-fei.com} 16, 28 -- To: {microscopy-at-microscopy.com} 16, 28 -- X-OriginalArrivalTime: 04 Sep 2008 18:08:29.0572 (UTC) FILETIME=[3C08D440:01C90EB9] 16, 28 -- Content-Transfer-Encoding: 8bit 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m84I8Yb8023492 ==============================End of - Headers==============================
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Email: degraef-at-cmu.edu Name: Marc De Graef
Organization: Carnegie Mellon University
Title-Subject: [Filtered] Classified Ad for microscopy Research Faculty position at CMU
Question: Research Faculty Position Department of Materials Science and Engineering Carnegie Mellon University
The Department of Materials Science and Engineering at Carnegie Mellon University seeks to fill a Research Faculty Position in the area of materials characterization. The successful candidate must have a Ph.D. in Materials Science, Physics, Chemistry, or a closely related field, and must demonstrate extensive expertise in the area of materials characterization, including both transmission electron microscopy (TEM) and scanning electron microscopy (SEM), and the underlying theory of electron scattering and image formation.
The candidate should ideally be capable of creating and managing funded research programs, collaborating with other research groups, establishing industrial relations, advising/teaching students and post-doctoral researchers as appropriate, and establishing an international reputation for his/her research accomplishments through publications and lectures. The candidate will be expected to participate actively in the daily operations (STEM, HRTEM, OIM, EDS/WDS, EELS, FIB, Ö) of the J. Earle and Mary Roberts Materials Characterization Laboratory. The candidate should have good interpersonal as well as oral and written communication skills.
Qualified candidates should send the following three items as electronic attachments (PDF format only) to a single email message to microscopy-search-at-andrew.cmu.edu :
ï a one page letter of application, including research interests; ï curriculum vitae, including publication list and names of three references; ï copies of three recent and pertinent publications.
Please reduce the PDF file size as much as possible and place your full name in the email subject line.
Review of applications will be ongoing.
CMU is an Equal Opportunity/Affirmative Action employer committed to building a diverse faculty; women and minorities are strongly encouraged to apply for this position.
The discussion on upgrading software raises some uncomfortable issues for both EM users and manufacturers. The obsoletion of a microscope because of its software is becoming depressingly familiar. I see this in our lab - the 'old' non-PC run microscope are working fine and are still going strong despite being commissioned in the 1970's and 80's. The modern PC-run FEGTEMs are starting to become obsolete because the operating systems used to run the detectors are now unsupported and therefore open to hacking and viruses. Transfer of data now has to go by floppy or Zip-disc! It won't be long before we have to have a succession of computers to convert and transfer the data to more manageable software. A rather ridiculous state of affairs if ever there was one.
Some EM groups do seem to manage to transfer their systems to newer computer platforms simply because they have excellent technical support who understand electronics, but these people are increasingly rare (and most seem close to retirement). An example of this type of person is anyone who is currently running a VG STEM on a modern computer.
I take Jan's point that the microscope configuration is almost unique amongst all those sold, but there are similarities between them, namely the electronic communication logic (e.g. TTL) used to control a third party detector from the main microscope PC. There must be sufficiently similar for a degree of commonality between microscopes there surely?
I cannot help but see an emerging market for electronic & software experts/ consultants, who have the technical expertise, to effectively re-engineer the driver/acquisition boards so that old existing microscopes are kept running well into the 21st century. Need it be that difficult or expensive?
==============================Original Headers============================== 6, 26 -- From jsb43-at-hermes.cam.ac.uk Fri Sep 5 11:43:00 2008 6, 26 -- Received: from ppsw-5.csi.cam.ac.uk (ppsw-5.csi.cam.ac.uk [131.111.8.135]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85Gh0X4008527 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 11:43:00 -0500 6, 26 -- X-Cam-AntiVirus: no malware found 6, 26 -- X-Cam-SpamDetails: not scanned 6, 26 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 6, 26 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:56103) 6, 26 -- by ppsw-5.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.155]:25) 6, 26 -- with esmtpa (EXTERNAL:jsb43) id 1KbeOh-0003dU-Ih (Exim 4.70) for Microscopy-at-microscopy.com 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 6, 26 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 6, 26 -- with local (PRAYER:jsb43) id 1KbeOh-0002Ah-PM (Exim 4.67) for Microscopy-at-microscopy.com 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 6, 26 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 6, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com} 6, 26 -- Subject: RE: FEI software 6, 26 -- Date: 05 Sep 2008 17:42:59 +0100 6, 26 -- X-Mailer: Prayer v1.2.3 6, 26 -- X-Originating-IP: [131.111.102.18] 6, 26 -- In-Reply-To: {200809041813.m84IDqlp031062-at-ns.microscopy.com} 6, 26 -- Message-ID: {Prayer.1.2.3.0809051742590.18714-at-hermes-1.csi.cam.ac.uk} 6, 26 -- References: {200809041813.m84IDqlp031062-at-ns.microscopy.com} 6, 26 -- Mime-Version: 1.0 6, 26 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 6, 26 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
} The modern PC-run FEGTEMs are starting to become obsolete because the } operating systems used to run the detectors are now unsupported and } therefore open to hacking and viruses.
Transfer of data now has to go } by floppy or Zip-disc!
That's why I wrote earlier today: "only real improvement of NT to W2K/XP is USB support". We have an "old" Leica TCS NT, still working fine, a bit slow maybe, and connecting it directly via network cards to another, modern (64bit) computer with blueray burner, network connection, 3D reconstruction software solves most of my problems... (like using MO- disks...) During acquisition you can even save the tif-files directly to the other computer, and the modern computer's firewall shields the old NT from the hazards of the modern internet world... And - the CLSM is for taking pictures, not surfing the web! And without USB, no student can use virus-infested USB-sticks...
It won't be long before we have to have a } succession of computers to convert and transfer the data to more } manageable software. A rather ridiculous state of affairs if ever } there was one.
Well, I do have problems to open old files from Commodore 64 and Sinclair Spectrum+ computers, but since the dawn of DOS at the beginning of the 1990s compatibility is not a problem anymore. Every year I buy a larger hard disk and just copy everything from A to B. And even if I have to run old programs like my favourite Win 3.1 astronomy software, I just install a virtual environment and use the original software.
That does not work with old hardware, though. That's the reason why I will probably switch to a Linux based OS, as soon as XP is not supported anymore.
} I cannot help but see an emerging market for electronic & software } experts/ consultants, who have the technical expertise, to effectively } re-engineer the driver/acquisition boards so that old existing } microscopes are kept running well into the 21st century. Need it be } that difficult or expensive?
Most drivers for Win2K work with XP, but hopefully the people responsible for the development of drivers will develop drivers for Linux in the future, too! Drivers for Linux become more and more an important issue to me - if I can decide between two different solutions, I buy the one with Linux support!
But things could be worse. It is still possible to buy modern, fast motherboards with ISA-slots, PCI won't die soon either, and EISA cards I don't use anymore (except the ones in the TCS NT). What I don't like is, that some vendors stop supporting 5 year old hardware and only offer some reduction on purchasing the new model...
;-) Torsten
==============================Original Headers============================== 18, 28 -- From Torsten.Fregin-at-zoologie.uni-hamburg.de Fri Sep 5 12:31:19 2008 18, 28 -- Received: from mailhost.uni-hamburg.de (mailhost.uni-hamburg.de [134.100.32.155]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85HVIHJ023771 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 12:31:19 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 4C5EF100BA1 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 19:31:18 +0200 (CEST) 18, 28 -- X-Virus-Scanned: by University of Hamburg (RRZ/mailhost) 18, 28 -- Received: from mailhost.uni-hamburg.de ([127.0.0.1]) 18, 28 -- by localhost (mailhost.uni-hamburg.de [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with LMTP id nJZF5-FMfKjI for {microscopy-at-microscopy.com} ; 18, 28 -- Fri, 5 Sep 2008 19:31:18 +0200 (CEST) 18, 28 -- Received: from [134.100.200.6] (TorstenF.zoologie.uni-hamburg.de [134.100.200.6]) 18, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 31E71100851 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 19:31:18 +0200 (CEST) 18, 28 -- From: Torsten.Fregin-at-zoologie.uni-hamburg.de 18, 28 -- To: microscopy-at-microscopy.com 18, 28 -- Date: Fri, 05 Sep 2008 19:31:57 +0200 18, 28 -- MIME-Version: 1.0 18, 28 -- Subject: Re: [Microscopy] RE: FEI software 18, 28 -- Message-ID: {48C1892D.9496.30962B-at-Torsten.Fregin.zoologie.uni-hamburg.de} 18, 28 -- Priority: normal 18, 28 -- In-reply-to: {200809051645.m85Gj5GC011637-at-ns.microscopy.com} 18, 28 -- References: {200809051645.m85Gj5GC011637-at-ns.microscopy.com} 18, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 18, 28 -- Content-type: text/plain; charset=US-ASCII 18, 28 -- Content-transfer-encoding: 7BIT 18, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
Prior to our upgrade to 2k, I had found a freeware utility provided by Dell for their laptops that allowed usb1 support on NT. I installed it on my non-laptop NT based FEI instrument. Worked flawlessly for all disk drives and flash drives we hooked up and one cd writer. Never tried mice, hubs, printers, etc. Only problem - we were never able to stop the disks prior to pulling them. Either had to shut down and remove safely, or like most of my users I just yanked them when done and clicked out of the dire data loss messages. Google R62200.exe or drop me an e-mail and I will pass along. File is about 5 megs and installed several things to make it work though It was so long ago I do not recall what. It was pretty much bulletproof.
Scott Whittaker Head NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
-----Original Message----- X-from: Torsten.Fregin-at-zoologie.uni-hamburg.de [mailto:Torsten.Fregin-at-zoologie.uni-hamburg.de] Sent: Friday, September 05, 2008 1:33 PM To: Whittaker, Scott
} The modern PC-run FEGTEMs are starting to become obsolete because the } operating systems used to run the detectors are now unsupported and } therefore open to hacking and viruses.
Transfer of data now has to go } by floppy or Zip-disc!
That's why I wrote earlier today: "only real improvement of NT to W2K/XP is USB support". We have an "old" Leica TCS NT, still working fine, a bit slow maybe, and connecting it directly via network cards to another, modern (64bit) computer with blueray burner, network connection, 3D reconstruction software solves most of my problems... (like using MO- disks...) During acquisition you can even save the tif-files directly to the other computer, and the modern computer's firewall shields the old NT from the hazards of the modern internet world... And - the CLSM is for taking pictures, not surfing the web! And without USB, no student can use virus-infested USB-sticks...
It won't be long before we have to have a } succession of computers to convert and transfer the data to more } manageable software. A rather ridiculous state of affairs if ever } there was one.
Well, I do have problems to open old files from Commodore 64 and Sinclair Spectrum+ computers, but since the dawn of DOS at the beginning of the 1990s compatibility is not a problem anymore. Every year I buy a larger hard disk and just copy everything from A to B. And even if I have to run old programs like my favourite Win 3.1 astronomy software, I just install a virtual environment and use the original software.
That does not work with old hardware, though. That's the reason why I will probably switch to a Linux based OS, as soon as XP is not supported anymore.
} I cannot help but see an emerging market for electronic & software } experts/ consultants, who have the technical expertise, to effectively } re-engineer the driver/acquisition boards so that old existing } microscopes are kept running well into the 21st century. Need it be } that difficult or expensive?
Most drivers for Win2K work with XP, but hopefully the people responsible for the development of drivers will develop drivers for Linux in the future, too! Drivers for Linux become more and more an important issue to me - if I can decide between two different solutions, I buy the one with Linux support!
But things could be worse. It is still possible to buy modern, fast motherboards with ISA-slots, PCI won't die soon either, and EISA cards I don't use anymore (except the ones in the TCS NT). What I don't like is, that some vendors stop supporting 5 year old hardware and only offer some reduction on purchasing the new model...
;-) Torsten
==============================Original Headers============================== 18, 28 -- From Torsten.Fregin-at-zoologie.uni-hamburg.de Fri Sep 5 12:31:19 2008 18, 28 -- Received: from mailhost.uni-hamburg.de (mailhost.uni-hamburg.de [134.100.32.155]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85HVIHJ023771 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 12:31:19 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 4C5EF100BA1 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 19:31:18 +0200 (CEST) 18, 28 -- X-Virus-Scanned: by University of Hamburg (RRZ/mailhost) 18, 28 -- Received: from mailhost.uni-hamburg.de ([127.0.0.1]) 18, 28 -- by localhost (mailhost.uni-hamburg.de [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with LMTP id nJZF5-FMfKjI for {microscopy-at-microscopy.com} ; 18, 28 -- Fri, 5 Sep 2008 19:31:18 +0200 (CEST) 18, 28 -- Received: from [134.100.200.6] (TorstenF.zoologie.uni-hamburg.de [134.100.200.6]) 18, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 31E71100851 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 19:31:18 +0200 (CEST) 18, 28 -- From: Torsten.Fregin-at-zoologie.uni-hamburg.de 18, 28 -- To: microscopy-at-microscopy.com 18, 28 -- Date: Fri, 05 Sep 2008 19:31:57 +0200 18, 28 -- MIME-Version: 1.0 18, 28 -- Subject: Re: [Microscopy] RE: FEI software 18, 28 -- Message-ID: {48C1892D.9496.30962B-at-Torsten.Fregin.zoologie.uni-hamburg.de} 18, 28 -- Priority: normal 18, 28 -- In-reply-to: {200809051645.m85Gj5GC011637-at-ns.microscopy.com} 18, 28 -- References: {200809051645.m85Gj5GC011637-at-ns.microscopy.com} 18, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 18, 28 -- Content-type: text/plain; charset=US-ASCII 18, 28 -- Content-transfer-encoding: 7BIT 18, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
==============================Original Headers============================== 27, 29 -- From WHITTAKS-at-si.edu Fri Sep 5 13:27:18 2008 27, 29 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85IRHQu013490 27, 29 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 13:27:17 -0500 27, 29 -- Received: from SI-MSESMTPO-02.US.SINET.SI.EDU (SI-MSESMTP-N2.us.sinet.si.edu [160.111.49.76]) 27, 29 -- by si-mailout04.si.edu (Postfix) with ESMTP id 1B8287D2A; 27, 29 -- Fri, 5 Sep 2008 14:24:36 -0400 (EDT) 27, 29 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by SI-MSESMTPO-02.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959); 27, 29 -- Fri, 5 Sep 2008 14:27:14 -0400 27, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 27, 29 -- Content-class: urn:content-classes:message 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Type: text/plain; 27, 29 -- charset="us-ascii" 27, 29 -- Subject: RE: [Microscopy] FEI software 27, 29 -- Date: Fri, 5 Sep 2008 14:27:13 -0400 27, 29 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA302CC534B-at-SI-ECL02.US.SINET.SI.EDU} 27, 29 -- In-Reply-To: {200809051732.m85HWemY026125-at-ns.microscopy.com} 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- Thread-Topic: [Microscopy] FEI software 27, 29 -- Thread-Index: AckPfWcd8oLXTncoSR2do+QdJL7cBgABA/uw 27, 29 -- References: {200809051732.m85HWemY026125-at-ns.microscopy.com} 27, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 27, 29 -- To: {Torsten.Fregin-at-zoologie.uni-hamburg.de} 27, 29 -- Cc: {microscopy-at-microscopy.com} 27, 29 -- X-OriginalArrivalTime: 05 Sep 2008 18:27:14.0310 (UTC) FILETIME=[04D7FA60:01C90F85] 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m85IRHQu013490 ==============================End of - Headers==============================
Years ago I tried to find this solution, but did not know the right words for Google...
} } Prior to our upgrade to 2k, I had found a freeware utility provided by } Dell for their laptops that allowed usb1 support on NT. } Google R62200.exe or } drop me an e-mail and I will pass along. File is about 5 megs and } installed several things to make it work though It was so long ago I } do not recall what. It was pretty much bulletproof.
==============================Original Headers============================== 6, 28 -- From Torsten.Fregin-at-zoologie.uni-hamburg.de Fri Sep 5 13:49:08 2008 6, 28 -- Received: from mailhost.uni-hamburg.de (mailhost.uni-hamburg.de [134.100.32.155]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85In7kC027883 6, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 13:49:07 -0500 6, 28 -- Received: from localhost (localhost [127.0.0.1]) 6, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 598B11008E8 6, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 20:49:07 +0200 (CEST) 6, 28 -- X-Virus-Scanned: by University of Hamburg (RRZ/mailhost) 6, 28 -- Received: from mailhost.uni-hamburg.de ([127.0.0.1]) 6, 28 -- by localhost (mailhost.uni-hamburg.de [127.0.0.1]) (amavisd-new, port 10024) 6, 28 -- with LMTP id 7L4Z2GtoMHcE for {Microscopy-at-microscopy.com} ; 6, 28 -- Fri, 5 Sep 2008 20:49:07 +0200 (CEST) 6, 28 -- Received: from [134.100.200.6] (TorstenF.zoologie.uni-hamburg.de [134.100.200.6]) 6, 28 -- by mailhost.uni-hamburg.de (Postfix) with ESMTP id 39E29100851 6, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 20:49:07 +0200 (CEST) 6, 28 -- From: Torsten.Fregin-at-zoologie.uni-hamburg.de 6, 28 -- To: Microscopy-at-microscopy.com 6, 28 -- Date: Fri, 05 Sep 2008 20:49:46 +0200 6, 28 -- MIME-Version: 1.0 6, 28 -- Subject: Re: [Microscopy] RE: FEI software 6, 28 -- Message-ID: {48C19B6A.23842.11FF5C-at-Torsten.Fregin.zoologie.uni-hamburg.de} 6, 28 -- Priority: normal 6, 28 -- In-reply-to: {200809051829.m85ITWNx016938-at-ns.microscopy.com} 6, 28 -- References: {200809051829.m85ITWNx016938-at-ns.microscopy.com} 6, 28 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 28 -- Content-type: text/plain; charset=US-ASCII 6, 28 -- Content-transfer-encoding: 7BIT 6, 28 -- Content-description: Mail message body ==============================End of - Headers==============================
} I take Jan's point that the microscope configuration is almost unique } amongst all those sold, but there are similarities between them, namely the } electronic communication logic (e.g. TTL) used to control a third party } detector from the main microscope PC. There must be sufficiently similar } for a degree of commonality between microscopes there surely?
Surely they are more similar than different. The software engineers are NOT writing unique code for MOST of the scopes sold/installed. There are all sorts of capabilities in our software that we don't USE while others do, but it's not a different version.
} I cannot help but see an emerging market for electronic & software experts/ } consultants, who have the technical expertise, to effectively re-engineer } the driver/acquisition boards so that old existing microscopes are kept } running well into the 21st century. Need it be that difficult or expensive?
Too bad that the manufacturers have little incentive to do this, though the R&D money required to do so would be better spent on a central project than individuals trying to do upgrades.
Jay
On Fri, Sep 5, 2008 at 11:44 AM, {jsb43-at-cam.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } The discussion on upgrading software raises some uncomfortable issues for } both EM users and manufacturers. The obsoletion of a microscope because of } its software is becoming depressingly familiar. I see this in our lab - the } 'old' non-PC run microscope are working fine and are still going strong } despite being commissioned in the 1970's and 80's. The modern PC-run } FEGTEMs are starting to become obsolete because the operating systems used } to run the detectors are now unsupported and therefore open to hacking and } viruses. Transfer of data now has to go by floppy or Zip-disc! It won't be } long before we have to have a succession of computers to convert and } transfer the data to more manageable software. A rather ridiculous state of } affairs if ever there was one. } } Some EM groups do seem to manage to transfer their systems to newer } computer platforms simply because they have excellent technical support who } understand electronics, but these people are increasingly rare (and most } seem close to retirement). An example of this type of person is anyone who } is currently running a VG STEM on a modern computer. } } I take Jan's point that the microscope configuration is almost unique } amongst all those sold, but there are similarities between them, namely the } electronic communication logic (e.g. TTL) used to control a third party } detector from the main microscope PC. There must be sufficiently similar } for a degree of commonality between microscopes there surely? } } I cannot help but see an emerging market for electronic & software experts/ } consultants, who have the technical expertise, to effectively re-engineer } the driver/acquisition boards so that old existing microscopes are kept } running well into the 21st century. Need it be that difficult or expensive? } } } ==============================Original Headers============================== } 6, 26 -- From jsb43-at-hermes.cam.ac.uk Fri Sep 5 11:43:00 2008 } 6, 26 -- Received: from ppsw-5.csi.cam.ac.uk (ppsw-5.csi.cam.ac.uk [131.111.8.135]) } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85Gh0X4008527 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 11:43:00 -0500 } 6, 26 -- X-Cam-AntiVirus: no malware found } 6, 26 -- X-Cam-SpamDetails: not scanned } 6, 26 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ } 6, 26 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:56103) } 6, 26 -- by ppsw-5.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.155]:25) } 6, 26 -- with esmtpa (EXTERNAL:jsb43) id 1KbeOh-0003dU-Ih (Exim 4.70) for Microscopy-at-microscopy.com } 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 } 6, 26 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) } 6, 26 -- with local (PRAYER:jsb43) id 1KbeOh-0002Ah-PM (Exim 4.67) for Microscopy-at-microscopy.com } 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 } 6, 26 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} } 6, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com} } 6, 26 -- Subject: RE: FEI software } 6, 26 -- Date: 05 Sep 2008 17:42:59 +0100 } 6, 26 -- X-Mailer: Prayer v1.2.3 } 6, 26 -- X-Originating-IP: [131.111.102.18] } 6, 26 -- In-Reply-To: {200809041813.m84IDqlp031062-at-ns.microscopy.com} } 6, 26 -- Message-ID: {Prayer.1.2.3.0809051742590.18714-at-hermes-1.csi.cam.ac.uk} } 6, 26 -- References: {200809041813.m84IDqlp031062-at-ns.microscopy.com} } 6, 26 -- Mime-Version: 1.0 } 6, 26 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 } 6, 26 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} } ==============================End of - Headers==============================
While we are on the subject of old computers, (and thanks for that NT/USB fix) does anyone have a work around for old ISA interface cards that have switch selectable port settings? Newer Windows operating systems will only support plug-and-play cards. I tried purchasing an ISA to USB interface but it didn't work and they specifically didn't offer tech support.
All of my equipment is purchased used with no chance of upgrading so I have to make do with what I have. This ISA card problem seems to be the bottleneck.
Thanks!
Tom Kaye
==============================Original Headers============================== 7, 21 -- From tom-at-TomKaye.com Fri Sep 5 14:19:43 2008 7, 21 -- Received: from w2k3-exchfe.mmsasp.local (mx1.mmsasp.com [66.102.127.13]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85JJhT1022375 7, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 14:19:43 -0500 7, 21 -- Received: from TKdual ([67.134.109.68]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 21 -- Fri, 5 Sep 2008 14:19:42 -0500 7, 21 -- From: "Tom Kaye" {tom-at-TomKaye.com} 7, 21 -- To: {Microscopy-at-microscopy.com} 7, 21 -- Subject: solving old computer problems, ISA cards?? 7, 21 -- Date: Fri, 5 Sep 2008 12:17:39 -0700 7, 21 -- Message-ID: {GOEAIJOJDGCJBPIFHOBGKEHCPMAA.tom-at-tomkaye.com} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="iso-8859-1" 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Priority: 3 (Normal) 7, 21 -- X-MSMail-Priority: Normal 7, 21 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 7, 21 -- Importance: Normal 7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 7, 21 -- X-OriginalArrivalTime: 05 Sep 2008 19:19:42.0943 (UTC) FILETIME=[59933EF0:01C90F8C] ==============================End of - Headers==============================
I've used the Model 623 and I am familiar with it, even though I now work for a competitor of Gatan.
Since you took it apart and the threads appear good, it sounds like the problem is not the polisher. If the threads are freely turning and they are not stripped, there is no physical way that they can make a linear jump to an extended position. But first, please check your stubs to make sure that they slide freely in and out of the hole and all the way to the bottom and that there is no excess wax on the sides of the stub. One problem that you can have with the Gatan 623 is that the SS stubs can slide too easily. This can be cured by scoring the sides of the stub with a sharp wire cutter. The Pyrex stubs generally don't have the same problem. If you have a dial or digital indicator, you can check the operation of the grinder by turning it upside down and checking the linear motion by turning the knob. Get a machinist or a mechanic to help you if you don't have the indicator tool. (Go to a brake shop and they would have the correct tools.) If that checks out, then there is nothing wrong with the grinder. Make sure that the knob is not slipping on the shaft when you turn it. Also, there is a push rod for pushing the stub out. Do not use the grinder with that in the hole. Take it out, you could be accidentally pushing the sample down while you use the fixture.
You probably need to supply some extra information. What is your sample? What are you using for abrasive? What are you using for lubrication? What are you trying to achieve -polished final sample or thinning for TEM? How are you affixing your samples to the stub, i.e. what type of wax are you using?
For hand grinding/lapping fixtures with micrometer control, there are two primary types. The first is the type that pushes the sample out as you turn the knob. The Gatan model 623 and the EA Fischione model 160 tools are examples of these. When you use these tools, the sample is pushed below the surface of the bottom of the fixture so that the fixture will actually wobble if the sample is pushed out to far. In this case, the whole weight of the fixture plus any downward force that you exert on the fixture, which you shouldn't do, is placed on the sample. When the sample is ground down until it is parallel to the bottom of the fixture, grinding of the sample stops. With this type of tool, the wax or glue that is used is under a lot of shear forces and it has to hold. In your case, you have to check that the wax is securely holding your sample. If you are dry grinding, the sample can get quite hot and that could soften the wax to change the working surface of your sample. If you are using a lubricant different than water, then make sure that it is not softening the wax. I also recommend that the fixture is not resting on the abrasive surface when you make your adjustment. You are statically pushing your sample into the abrasive which is not good.
The other type of fixture is the piston type exemplified by the South Bay Technology lapping fixtures, e.g. our model 150. In these, the sample is mounted on a block that is fastened to a piston that slides in the body of the fixture. The load on the sample is the weight of the piston, mounting block, and any additional weight that is put on top. These fixtures can be made to have a set of springs that offset the weight of the piston and mounting block for very light load polishing applications. The feet of the body establishes a polishing plane that does not rock and the piston/sample combinations falls as the sample is ground down until it comes to the limit set by the micrometer knob. These type of fixtures also can be used as the first type of fixtures above if the piston is locked in position with the sample set below the plane of the feet. These fixtures more readily lend themselves to automatic polishing stations compared to the first type because they can be set with a larger amount of material to be taken off and they don't have the rocking problem. Since the sample does not rock or have as high a load applied to it during the grinding these types of fixtures are less likely to have the problems with the high shear forces on the wax. One disadvantage of the piston type of fixtures is that hand grinding in the free piston mode is slower unless comparable weight is applied. Another disadvantage to these is that the piston could stick. Our fixtures have a dry lubricant coating on the piston. If the surface of that coating oxides because of the presence of moisture, it needs to be removed by simply wiping the piston with a dry cloth.
That reminds of a strict rule that novice users quite often forget -never turn a wet grinding/lapping fixture upside down. In the case of the first style (Gatan and Fischione) tools, the water can reach the threads and corrode them over time. In the case of ours, the solid film lubricant will oxidize and needs to be cleaned. Always dry the fixture while it is in the upright position before turning it upside down.
I'm not sure that I answered your question or not, but I thought that I would use the opportunity to discuss hand grinding and lapping. We have made a DVD that discusses various aspects of lapping. It features Earnest Apodoca, an expert in lapping, interacting with one of our customers to solve his lapping problems. It discusses the procedures for measuring and truing the lapping plate, truing the polishing feet and mounting blocks, how to measure flatness, zeroing the digital lapping fixture, obtaining a precise thickness, and controlling the damage depth during polishing. If you are interested in this lapping DVD, you can request a free copy. We make it available to people that are having problems lapping or who are considering doing demanding lapping.
Disclaimer: SBT makes and sells lapping fixtures.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mcavaliere-at-mvainc.com [mailto:mcavaliere-at-mvainc.com] Sent: Thursday, September 04, 2008 6:17 PM To: Walck-at-SouthBayTech.com
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Question: We have a Gatan Model 623 Disc Grinder which does not appear to be advancing the sample correctly. Polishing seems to be going along smoothly and then all of a sudden with a turn of 5 microns or less the sample catches and is frequently pulled off the specimen mount and/or severely damaged. If the sample remains mounted, it is now sitting 10 to 100+ microns above the plane of the disc grinder! This seems to occur regardless of the grit of the paper, the RPMs of the polisher, and the sample material. We have taken the disc grinder apart and the threads do not look damaged. We have also replaced the o-ring and the spring. Any ideas on what might be going on and/or how to solve this?
Thanks,
Michelle Ritorto Cavaliere, Ph.D. Senior Research Scientist MVA Scientific Consultants, Inc. 3300 Breckinridge Blvd., Suite 400 Duluth, GA 30096 Phone: 770-662-8509 Fax: 770-662-8532
Perhaps I should title this history and predictions.
The issue of upgrades is a serious topic and a situation that can only get worse with time, IMO. I see this being broken down into two parts. One is support for the EM system while the other is upgrades.
When a tool is purchased new, it usually comes with one year of maintenance. This culls out the infant mortality issues. After this year is up, not getting and keeping a maintenance contract can be an expensive mistake. Per diem rates are high and parts are at your expense. The way newer (now to about ten years ago) are made at the electronics level are with surface mounted components. This means repair is done via board swap out. Older tools had socketed ICs and could be rather easily fixed. The older tools also typically came with full schematics. Not so for current tools. Either they are not released or they don't reflect a generation change. E.g., LEO 1550 schematics exist but LEO/Supra do not, even though the Supra is based on the 1550 series. Other brands have similar issues and some probably do not.
Zeiss will support a system for ten years after it is out of production. For the 15xx series, they have a major upgrade that changes PC boards and upgrades the in-lens detector. I think that it also changes the PC and SmartSEM.
The new construction methods mean more functions exist and these functions are all in the plinth. The control is the PC. While the older tools used embedded processors, the control was via knobs. Later, some added external PC control as well as knob control (Amray 1800 and onwards for example). The control of the SEM via PC is maker specific. Some use SE SCSI while others use LVD SCSI-II. Amray used NibbleNet (their own unique method of control interface).
The move to total PC control is really a key issue. The early PCs used all ISA slots. Later motherboards had ISA and PCI. Then, as now, only PCI. I've seen some MBs that have at least one ISA slot but these probably won't last long. The OS for the early PCs started with Win 3.1 and 3.11 (that is as far back as I go for PC controlled SEMs and FIBs). The first major Windows release was Win95. It was a good improvement over 3.x. Right on its heels was Win98 and then Win98SE (first version for USB support). This was a good desktop OS. Then it started being used for EM control. OK. Then NT came out. NT was written by the same folks who created DEC's VAX/VMS. VMS was a joy to use. On a VAX/7xx, the CPU microcode was loaded at each boot via floppy or tape (this was pre-FPGA days!). Then the 730 and 750 came out followed by the MicroVAX. So what? The file system for VMS was radical. Kernel-level apps could be written and wrapped.
The file system for PDP-11 using RT-11 was contiguous sectors as was RSX-11. Memory management was either non-existent or marginal. VMS changed all of that with virtual memory structure. It also enhanced the dynamic location of drivers and apps where before they had to be hard coded for specific memory locations. I think that RT-11 was the first to support relocatable code. This was very handy for large applications that needed only portions at a time. Pieces of the app could be swapped in and out as necessary.
The file system (ala, NTFS) used double linked sectors, privileges, security, and much more. Not to mention the robust memory management now available in the newer processors. Win98 used up to 328MB of physical RAM no matter how much was installed. NT changed that. If one did not know the file system structure, there was no chance at writing kernel-level modules. But this supported wrappers really well.
So, where does that leave us? I think, heading for disaster unless and until some tool maker makes a big strategic change. That change is to drop Windows as the controlling OS and use Linux. It has X Windows GUI and runs on many platforms. Yes, re-porting control apps and drivers is not trivial. Linux has excellent memory management and virtual memory services. It was based on BBN and BSD Unix and has a solid history. It has good security features and has evolved to support many different hardware devices. This will of course impact the field service folks and users too for awhile.
I suppose the alternative is to revert to knobs and have a 300 conductor cable between a panel and the plinth.
Sorry for the long posting. If any of my history is off, please let me know.
gary g.
==============================Original Headers============================== 15, 17 -- From gary-at-gaugler.com Fri Sep 5 15:42:23 2008 15, 17 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 15, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m85KgN66018993 15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 15:42:23 -0500 15, 17 -- Message-Id: {200809052042.m85KgN66018993-at-ns.microscopy.com} 15, 17 -- Received: (qmail 25603 invoked from network); 5 Sep 2008 13:42:26 -0700 15, 17 -- Received: by simscan 1.1.0 ppid: 25600, pid: 25601, t: 0.1376s 15, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 15, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 15, 17 -- by smtp2 with SMTP; 5 Sep 2008 13:42:26 -0700 15, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 17 -- Date: Fri, 05 Sep 2008 13:42:15 -0700 15, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 15, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 15, 17 -- Subject: Legacy EM and the future (long) 15, 17 -- Mime-Version: 1.0 15, 17 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-1E53131 ==============================End of - Headers==============================
Thanks for the motherboard suggestions but that is not the problem. The new operating systems will not allow you to use a switch-selectable-port ISA card even if you have it plugged into a good, working, ISA slot. I can not upgrade the OS from Win95 on my FTIR spectrometer because the interface card uses switches to set the port and XP etc will not support that. If anyone has figured out a way to use these cards with modern operating systems I would sure love to know.
Thanks,
Tom
-----Original Message----- X-from: Schoel [mailto:schoel-at-ucalgary.ca] Sent: Friday, September 05, 2008 12:59 PM To: tom-at-TomKaye.com
The key issue which many of us tend to misunderstand is that, contrarily to what marketing managers would tell you, OEMs are not (primarily) in the business of manufacturing and supporting equipment - these are commercial organizations which are first and foremost in the business of making money, otherwise they simply could not exist. The downside of this reality of life is that if there are more money to be made (or a misperception of such) by obsolescing equipment, or by dropping entire product lines rather then by developing them, then OEM support will be dropped.
Most of OEMs are large, inverted-pyramid organizations with heavy overhead and complicated internal politics, making any effort for them is very difficult and costly. Unless there are enough customers who are willing to pay top dollars for (for example) upgrading to newer operating system, or unless there are significant and imminent losses from not doing so, then OEM simply can't afford to (and never would) spend resources, however small, on developing, testing and distributing the upgrade. At times it is more cost-effective for OEM to allocate resources for "proving" that the upgrade is not possible, then on actually making it happen.
Regarding the mentioned difficulty and expense of re-engineering electronics and drivers, or even refurbishment of entire systems - technically everything can be done. I recall that some of the third-party service providers, who are focused on particular types of the equipment for long time, even have (or had) "off the shelf" upgrades for SEM electronics. If your own technical support does not "understand electronics" - take a look on third-party service options.
Financially, rather often then not, rebuilding of obsolete electronics, reconfiguring computer platforms, and even updating electron optics costs less then commissioning of the entire new instrument from OEM, but such re-engineering projects still have a price tag much higher then ordinary repair; engineering effort is larger and good engineers are not cheap.
Some market for re-engineering service does exist, but in my experience users are often have less difficulty to justify commissioning of the entire new instrument then getting funding for refurbishment of the old tool for a fraction of the cost; re-engineering of interface boards and controllers seems to happen more often.
Have a great weekend everyone :) Valery Ray
============================ www.partbeamsystech.com www.freudlabs.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
Disclaimer: PBS&T is in the business of development, service, and support of SEM and FIB equipment and thus my opinion can be (and probably is) biased...
-----Original Message----- X-from: jsb43-at-cam.ac.uk [mailto:jsb43-at-cam.ac.uk] Sent: Friday, September 05, 2008 12:44 PM To: vray-at-partbeamsystech.com
Dear All,
The discussion on upgrading software raises some uncomfortable issues for both EM users and manufacturers. The obsoletion of a microscope because of its software is becoming depressingly familiar. I see this in our lab - the 'old' non-PC run microscope are working fine and are still going strong despite being commissioned in the 1970's and 80's. The modern PC-run FEGTEMs are starting to become obsolete because the operating systems used to run the detectors are now unsupported and therefore open to hacking and viruses. Transfer of data now has to go by floppy or Zip-disc! It won't be long before we have to have a succession of computers to convert and transfer the data to more manageable software. A rather ridiculous state of affairs if ever there was one.
Some EM groups do seem to manage to transfer their systems to newer computer platforms simply because they have excellent technical support who understand electronics, but these people are increasingly rare (and most seem close to retirement). An example of this type of person is anyone who is currently running a VG STEM on a modern computer.
I take Jan's point that the microscope configuration is almost unique amongst all those sold, but there are similarities between them, namely the electronic communication logic (e.g. TTL) used to control a third party detector from the main microscope PC. There must be sufficiently similar for a degree of commonality between microscopes there surely?
I cannot help but see an emerging market for electronic & software experts/ consultants, who have the technical expertise, to effectively re-engineer the driver/acquisition boards so that old existing microscopes are kept running well into the 21st century. Need it be that difficult or expensive?
==============================Original Headers============================== 6, 26 -- From jsb43-at-hermes.cam.ac.uk Fri Sep 5 11:43:00 2008 6, 26 -- Received: from ppsw-5.csi.cam.ac.uk (ppsw-5.csi.cam.ac.uk [131.111.8.135]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85Gh0X4008527 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 11:43:00 -0500 6, 26 -- X-Cam-AntiVirus: no malware found 6, 26 -- X-Cam-SpamDetails: not scanned 6, 26 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 6, 26 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:56103) 6, 26 -- by ppsw-5.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.155]:25) 6, 26 -- with esmtpa (EXTERNAL:jsb43) id 1KbeOh-0003dU-Ih (Exim 4.70) for Microscopy-at-microscopy.com 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 6, 26 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 6, 26 -- with local (PRAYER:jsb43) id 1KbeOh-0002Ah-PM (Exim 4.67) for Microscopy-at-microscopy.com 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 6, 26 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 6, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com} 6, 26 -- Subject: RE: FEI software 6, 26 -- Date: 05 Sep 2008 17:42:59 +0100 6, 26 -- X-Mailer: Prayer v1.2.3 6, 26 -- X-Originating-IP: [131.111.102.18] 6, 26 -- In-Reply-To: {200809041813.m84IDqlp031062-at-ns.microscopy.com} 6, 26 -- Message-ID: {Prayer.1.2.3.0809051742590.18714-at-hermes-1.csi.cam.ac.uk} 6, 26 -- References: {200809041813.m84IDqlp031062-at-ns.microscopy.com} 6, 26 -- Mime-Version: 1.0 6, 26 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 6, 26 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
"Be careful what you ask for, you just may get it...."
Remember the day when the electron microscopes we used we analog and mixed analog/digital in their design. One had infinite control of all of the functions, not controlled by discreet bits and bytes of an operating system. One could easily upgrade to the latest EDS or Image Acquisition System with little fuss and be considered "state of the art". Ah, the "good ol' days". But, with the new generation of MAC & PC educated researchers coming online in the 80's & 90's, they wanted instrumentation that they didn't have to babysit(analog) - hence , the computer controlled electron microscope. It was and is a great idea, automating many mundane tasks so one could concentrate their time on one's research. And, the manufacturers responded en masse. They saw this era as one that would reap very good, if not great financial corporate rewards. Now, instead of an instrument lasting for 20, 30, even 40 years, being maintained by the manufacturer, in-house staff, or third party companies, this new, state of the art, PC based , Digital SEM/TEM(or whatever) now only lasts for 5 to 10 years(maybe), because of some guy named Moore(Moore's Law). They were/are now selling many times more instruments/system than they did in the past, didn't have to worry about keeping obsolete repair stock(for 20 years) and they cut out the in-house/third party service organizations - another big money maker, everything was sole sourced to the manufacturer, from the initial sale to the on going service. Ching Ching! Hardware development in all areas run at an extremely blistering pace and when one designs to run a certain set of hardware with a popular Operating System, it is essentially outdated when the instrument comes to market(see Moore's Law). This is why seemingly simple and inexpensive software upgrades are not possible. Also, the way that the software "hooks" in the hardware changes radically for OS generation to generation, making it that the more difficult. It is EXTREMELY difficult if not impossible to re-engineer an older(5 years) instrument, unless the customer wants to bear the full brunt for re-engineering. In most cases, it is simply not worth it financially. Because much of the "code" is locked in the silicon itself, not one manufacturer's would be willing to give away the "keys to the kingdom" to an interested third party and there isn't enough sheer numbers of instruments to make it a viable(profitable) alternative. There is no happy medium. If the hardware and software became standardized, companies would lose any competitive edge in trying to sell their own, individual unique product, the end of the free market system(ha, just kidding!). So, it seems we all must follow the technology "Pied Piper" into the future. It all just boils down to the dollars..........
"Be careful what you ask for, you just may get it...."
Gary Easton
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} I take Jan's point that the microscope configuration is almost unique } amongst all those sold, but there are similarities between them, namely the } electronic communication logic (e.g. TTL) used to control a third party } detector from the main microscope PC. There must be sufficiently similar } for a degree of commonality between microscopes there surely?
Surely they are more similar than different. The software engineers are NOT writing unique code for MOST of the scopes sold/installed. There are all sorts of capabilities in our software that we don't USE while others do, but it's not a different version.
} I cannot help but see an emerging market for electronic & software experts/ } consultants, who have the technical expertise, to effectively re-engineer } the driver/acquisition boards so that old existing microscopes are kept } running well into the 21st century. Need it be that difficult or expensive?
Too bad that the manufacturers have little incentive to do this, though the R&D money required to do so would be better spent on a central project than individuals trying to do upgrades.
Jay
On Fri, Sep 5, 2008 at 11:44 AM, {jsb43-at-cam.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } The discussion on upgrading software raises some uncomfortable issues for } both EM users and manufacturers. The obsoletion of a microscope because of } its software is becoming depressingly familiar. I see this in our lab - the } 'old' non-PC run microscope are working fine and are still going strong } despite being commissioned in the 1970's and 80's. The modern PC-run } FEGTEMs are starting to become obsolete because the operating systems used } to run the detectors are now unsupported and therefore open to hacking and } viruses. Transfer of data now has to go by floppy or Zip-disc! It won't be } long before we have to have a succession of computers to convert and } transfer the data to more manageable software. A rather ridiculous state of } affairs if ever there was one. } } Some EM groups do seem to manage to transfer their systems to newer } computer platforms simply because they have excellent technical support who } understand electronics, but these people are increasingly rare (and most } seem close to retirement). An example of this type of person is anyone who } is currently running a VG STEM on a modern computer. } } I take Jan's point that the microscope configuration is almost unique } amongst all those sold, but there are similarities between them, namely the } electronic communication logic (e.g. TTL) used to control a third party } detector from the main microscope PC. There must be sufficiently similar } for a degree of commonality between microscopes there surely? } } I cannot help but see an emerging market for electronic & software experts/ } consultants, who have the technical expertise, to effectively re-engineer } the driver/acquisition boards so that old existing microscopes are kept } running well into the 21st century. Need it be that difficult or expensive? } } } ==============================Original Headers============================== } 6, 26 -- From jsb43-at-hermes.cam.ac.uk Fri Sep 5 11:43:00 2008 } 6, 26 -- Received: from ppsw-5.csi.cam.ac.uk (ppsw-5.csi.cam.ac.uk [131.111.8.135]) } 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m85Gh0X4008527 } 6, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 11:43:00 -0500 } 6, 26 -- X-Cam-AntiVirus: no malware found } 6, 26 -- X-Cam-SpamDetails: not scanned } 6, 26 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ } 6, 26 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:56103) } 6, 26 -- by ppsw-5.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.155]:25) } 6, 26 -- with esmtpa (EXTERNAL:jsb43) id 1KbeOh-0003dU-Ih (Exim 4.70) for Microscopy-at-microscopy.com } 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 } 6, 26 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) } 6, 26 -- with local (PRAYER:jsb43) id 1KbeOh-0002Ah-PM (Exim 4.67) for Microscopy-at-microscopy.com } 6, 26 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Sep 2008 17:42:59 +0100 } 6, 26 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} } 6, 26 -- To: MSA Listserver {Microscopy-at-microscopy.com} } 6, 26 -- Subject: RE: FEI software } 6, 26 -- Date: 05 Sep 2008 17:42:59 +0100 } 6, 26 -- X-Mailer: Prayer v1.2.3 } 6, 26 -- X-Originating-IP: [131.111.102.18] } 6, 26 -- In-Reply-To: {200809041813.m84IDqlp031062-at-ns.microscopy.com} } 6, 26 -- Message-ID: {Prayer.1.2.3.0809051742590.18714-at-hermes-1.csi.cam.ac.uk} } 6, 26 -- References: {200809041813.m84IDqlp031062-at-ns.microscopy.com} } 6, 26 -- Mime-Version: 1.0 } 6, 26 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 } 6, 26 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} } ==============================End of - Headers==============================
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Question: I may be a bit slow this morning but I am having a hard time seeing an easy way to go about changing the chamber backing light on our Tousimis Autosamdri 815B. Right now the leading hypotheses are to remove the sample chamber or remove the bottom plate.
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I don't foresee the hardware ever being standardized. Each company has a different approach to how they get from the e source to specimen. I just think that the software platform will have to change. In this regard, the EM makers are at the mercy of the OS makers. For the most part, Microsoft. MS does not care what the impact is on their customers or even their niche EM customers. Just sell more OS units. Well, that halted with Vista. A junk OS. XP is stable and pretty well done...albeit with continuing security holes. Why go to Vista with a hostile interface and a whole new set of security holes? Time to move on.
It seems to me that each maker can make their own electronic and physical incarnation of an EM but use a standard software platform using Linux. The real kicker is if someone makes a Linux-based Perl or Java app for EM. That would narrow down the interface issue to a few key system calls. So now one would have hardware to buy and then separate software to buy to run the hardware.
The user interface could be nearly the same for different hardware makers. But this would not require major changes in the software interface. Plus, this would easily eliminate the stupid hardware dongles to protect their hardware specific software. This "protection" is just an additional cost that users pay. With a common software interface, the competitive advantage moves to what the hardware can do. There is clearly a lot of wiggle room in this regard.
gary g.
At 02:25 PM 9/5/2008, you wrote:
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==============================Original Headers============================== 8, 20 -- From gary-at-gaugler.com Fri Sep 5 21:00:42 2008 8, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m8620fhD009231 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 21:00:41 -0500 8, 20 -- Message-Id: {200809060200.m8620fhD009231-at-ns.microscopy.com} 8, 20 -- Received: (qmail 24565 invoked from network); 5 Sep 2008 19:00:44 -0700 8, 20 -- Received: by simscan 1.1.0 ppid: 24562, pid: 24563, t: 0.1555s 8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 8, 20 -- by smtp2 with SMTP; 5 Sep 2008 19:00:44 -0700 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 20 -- Date: Fri, 05 Sep 2008 19:00:33 -0700 8, 20 -- To: garyeaston-at-scannerscorp.com 8, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 20 -- Subject: Re: [Microscopy] FEI software - Another take 8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 20 -- In-Reply-To: {200809052125.m85LPblc029145-at-ns.microscopy.com} 8, 20 -- References: {200809052125.m85LPblc029145-at-ns.microscopy.com} 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-59434434 ==============================End of - Headers==============================
I guess I will get on this soap box one more time...
If you have a system which is just hardware, and being controlled through a generic user interface, who would take responsibility for the system? I know we wouldn't...
We may go to Linux, after having already seen a number of VISTA tests fall flat on their faces. Who knows what tomorrow will bring? Maybe Macintosh is the way to go, or indeed Java?
X-from our point of view, it is the case that the microscope control system and the desktop OS is one aspect, the control system for the accessory manufacturers is another aspect. FEI, when they release an upgrade, release the Windos OS, the microsocpe control system and ALL accessory software that is needed, as one fully tested package; and as a result we are reasonably confident that this package will work properly in your system.
This is why we do not allow accessory manufacturers to update software on a Tecnai or Titan system, since they typically only test their own functionality, not the complete microscope system capabilities. So while to some people it may seem ludicrous that we are using accessory software that is three generations behind the current state of the art, watch these same people scream blue murder when the newest accessory SW is installed on their system, but now the microscope shutter no longer works, tomography doesn't function anymore and the low dose option has turned into the high dose option!
We have to test all of this before the final package is released, and this takes time. If an accessory manufacturer releases new SW to fix bugs, instead of fixing the existing SW with a patch or a service pack, this illustrates rushing to market without thorough testing for all applications. In such cases we have to do the testing for them. It is interesting that when we ask to get some imperfections or bugs fixed, the response is sometimes "we've already fixed that in the next version" but in that new version, all kinds of new bugs are present. That never leads to a stable system, and the testing becomes endless, with moving targets for the SW group; now THIS is a very frustrating experience.
All these things happen behind the closed doors of a company trying to make a system 'easy to use' and embed software from multiple vendors on to one platform. We still believe this is the best way to get systems at a higher uptime efficiency than those controlled by multiple vendors, but I agree it is a tough task to keep everyone happy. It has become clear, though, that a large number of users are not interested in the electron microscope, or the techniques, per-se, however everybody seems to be very interested in data. "Give me the data, I don't care how the system works" is a common theme. This poses a sizeable problem to us, since all users like the data to be correct.
I hope that by explaining this, it is clear that SW upgrades and OS upgrades cannot be done as part of the regular service contract, and that as a company we could not possibly survive if we did this free of charge. Some people ask for free SW upgrades; I would say if you really want a free upgrade, and you succeed to get a legitimate free upgrade of your OS from Microsoft, I will revisit this discussion.
On the other hand, if we get lots of good feedback about functionality, bugs, serviceability and/or capabilities of the system from some users, we would be much more inclined to encompass those users in beta-testing programs. We, as a company, need information about how systems are working in the field, both what is good as well as bad. Only if we get a good stream of information, can we make the right decisions about new SW directions and requirements. So keep the comments coming, they are being used... And to quote one of the Gary's: "Be careful what you wish for, you may just get it..."!
Jan
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I don't foresee the hardware ever being standardized. Each company has a different approach to how they get from the e source to specimen. I just think that the software platform will have to change. In this regard, the EM makers are at the mercy of the OS makers. For the most part, Microsoft. MS does not care what the impact is on their customers or even their niche EM customers. Just sell more OS units. Well, that halted with Vista. A junk OS. XP is stable and pretty well done...albeit with continuing security holes. Why go to Vista with a hostile interface and a whole new set of security holes? Time to move on.
It seems to me that each maker can make their own electronic and physical incarnation of an EM but use a standard software platform using Linux. The real kicker is if someone makes a Linux-based Perl or Java app for EM. That would narrow down the interface issue to a few key system calls. So now one would have hardware to buy and then separate software to buy to run the hardware.
The user interface could be nearly the same for different hardware makers. But this would not require major changes in the software interface. Plus, this would easily eliminate the stupid hardware dongles to protect their hardware specific software. This "protection" is just an additional cost that users pay. With a common software interface, the competitive advantage moves to what the hardware can do. There is clearly a lot of wiggle room in this regard.
gary g.
At 02:25 PM 9/5/2008, you wrote:
} ----------------------------------------------------------------------- } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
} didn't have to babysit(analog) - hence , the computer controlled } electron microscope. It was and is a great idea, automating many } mundane tasks so one could concentrate their time on one's research. } And, the manufacturers responded en masse. They saw this era as one } that would reap very good, if not great financial corporate rewards. } Now, instead of an instrument lasting for 20, 30, even 40 years, being } maintained by the manufacturer, in-house staff, or third party } companies, this new, state of the art, PC based , Digital SEM/TEM(or } whatever) now only lasts for 5 to 10 years(maybe), because of some guy } named Moore(Moore's Law). They were/are now selling many times more } instruments/system than they did in the past, didn't have to worry } about keeping obsolete repair stock(for 20 years) and they cut out the } in-house/third party service organizations - another big money maker, } everything was sole sourced to the manufacturer, from the initial sale } to the on going service. Ching Ching! } Hardware development in all areas run at an extremely blistering } pace and when one designs to run a certain set of hardware with a } popular Operating System, it is essentially outdated when the } instrument comes to market(see Moore's Law). This is why seemingly simple and } inexpensive software upgrades are not possible. Also, the way that } the software "hooks" in the hardware changes radically for OS } generation to generation, making it that the more difficult. } It is EXTREMELY difficult if not impossible to re-engineer an } older(5 years) instrument, unless the customer wants to bear the full } brunt for re-engineering. In most cases, it is simply not worth it } financially. Because much of the "code" is locked in the silicon } itself, not one manufacturer's would be willing to give away the "keys } to the kingdom" to an interested third party and there isn't enough } sheer numbers of instruments to make it a viable(profitable) alternative. } There is no happy medium. If the hardware and software became } standardized, companies would lose any competitive edge in trying to } sell their own, individual unique product, the end of the free market } system(ha, just kidding!). So, it seems we all must follow the } technology "Pied Piper" into the future. It all just boils down to the
} dollars.......... } } "Be careful what you ask for, you just may get it...." } } } Gary Easton
==============================Original Headers============================== 26, 30 -- From Jan.Ringnalda-at-fei.com Fri Sep 5 22:45:09 2008 26, 30 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m863j9kE024796 26, 30 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 22:45:09 -0500 26, 30 -- X-WSS-ID: 0K6R93D-01-H03-01 26, 30 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 26, 30 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 2113199F6DB; 26, 30 -- Fri, 5 Sep 2008 20:45:13 -0700 (PDT) 26, 30 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 26, 30 -- Fri, 5 Sep 2008 20:45:08 -0700 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 30 -- Content-class: urn:content-classes:message 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Type: text/plain; 26, 30 -- charset="us-ascii" 26, 30 -- Subject: RE: [Microscopy] Re: FEI software - Another take 26, 30 -- Date: Fri, 5 Sep 2008 20:45:06 -0700 26, 30 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB813C9BF5D-at-hlexc03.w2k.feico.com} 26, 30 -- In-Reply-To: {200809060206.m8626eBN022280-at-ns.microscopy.com} 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- Thread-Topic: [Microscopy] Re: FEI software - Another take 26, 30 -- Thread-Index: AckPxTUU5HbU6eFAQDKdfsR2CKWIRAABQTMA 26, 30 -- References: {200809060206.m8626eBN022280-at-ns.microscopy.com} 26, 30 -- From: "Ringnalda, Jan" {Jan.Ringnalda-at-fei.com} 26, 30 -- To: {gary-at-gaugler.com} 26, 30 -- Cc: {microscopy-at-microscopy.com} 26, 30 -- X-OriginalArrivalTime: 06 Sep 2008 03:45:08.0289 (UTC) FILETIME=[F4E3D310:01C90FD2] 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m863j9kE024796 ==============================End of - Headers==============================
I hope that when you install ISA card and boot computer Windows recognizes that it is present. If so, then you will need to tell Windows which IRQ and address space to use for your ISA card and take care of preventing conflicts with other devices. For basic ideas on configuring Windows and BIOS for legacy ISA card see
http://www.lavalink.com/index.php?id=655
you can also try to write .inf file and specify resources for your card, or pay someone for doing it (try www.rentacoder.com).
Good luck :)
Valery Ray
============================ www.partbeamsystech.com www.freudlabs.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: tom-at-TomKaye.com [mailto:tom-at-TomKaye.com] Sent: Friday, September 05, 2008 5:17 PM To: vray-at-partbeamsystech.com
Thanks for the motherboard suggestions but that is not the problem. The new operating systems will not allow you to use a switch-selectable-port ISA card even if you have it plugged into a good, working, ISA slot. I can not upgrade the OS from Win95 on my FTIR spectrometer because the interface card uses switches to set the port and XP etc will not support that. If anyone has figured out a way to use these cards with modern operating systems I would sure love to know.
Thanks,
Tom
-----Original Message----- X-from: Schoel [mailto:schoel-at-ucalgary.ca] Sent: Friday, September 05, 2008 12:59 PM To: tom-at-TomKaye.com
All,
One of my statements is being mis-interpreted.
With reference to the 'free' microsoft OS upgrades, let me expand one statement:
FEI does provide as part of warranty or service contract, free service packs, patches and minor revision SW upgrades. This means going from verison 1 all the way through to 1.9 is included, these upgrades may include functionality enhancement, but typically does not include HW upgrades. Going from SW version 1.X to 2.0 would be chargeable, because this includes acquisition card upgrades if needed, PC hardware as well operating system OS upgrade, and usually includes significant functionality enhancements.
Thanks, Jan
==============================Original Headers============================== 6, 29 -- From Jan.Ringnalda-at-fei.com Sat Sep 6 07:21:11 2008 6, 29 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m86CLBMe004423 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 6 Sep 2008 07:21:11 -0500 6, 29 -- X-WSS-ID: 0K6RWZB-01-XGJ-01 6, 29 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 6, 29 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 2A3E887CFEF 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 6 Sep 2008 05:21:10 -0700 (PDT) 6, 29 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Sat, 6 Sep 2008 05:21:09 -0700 6, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 29 -- Content-class: urn:content-classes:message 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="us-ascii" 6, 29 -- Subject: FEI software - "Free Upgrades" 6, 29 -- Date: Sat, 6 Sep 2008 05:21:08 -0700 6, 29 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB813C9C058-at-hlexc03.w2k.feico.com} 6, 29 -- In-Reply-To: {200809060352.m863qR09005408-at-ns.microscopy.com} 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- Thread-Topic: FEI software - "Free Upgrades" 6, 29 -- Thread-Index: AckP0/3aGi7eQZjnR3GDHSPQyz8yFgARTzow 6, 29 -- References: {200809060352.m863qR09005408-at-ns.microscopy.com} 6, 29 -- From: "Ringnalda, Jan" {Jan.Ringnalda-at-fei.com} 6, 29 -- To: {microscopy-at-microscopy.com} 6, 29 -- X-OriginalArrivalTime: 06 Sep 2008 12:21:09.0874 (UTC) FILETIME=[0B6EC520:01C9101B] 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m86CLBMe004423 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fishjuhl-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fishjuhl-at-yahoo.com Name: oldbones
Title-Subject: [Filtered] RCA EMU-3G sn 3497 parts
Question: I have found an OLD electron microscope. Some parts have been removed but most parts are accounted for. Also have cryo unit ultraviolet analyzer oxygen analyzer and many OLD science equipment parts. Most are museum pieces and some do work! Please help me find a good home for these. I will sell them for scrap on september 25 2008 to a local scrap yard.
I have a hoard of books--mostly from the 1970's to 1990's including EMSA, MSA and International proceedings as well as many of the Hayat books plus many others. They are all looking for good homes. Any interest? Am looking for individuals, libraries or used book sellers. Please contact at this email: rcmoretz-at-gmail.com.
Thanks
Roger Moretz (ret.)
PS The wife said it's time to downsize, and she's right.
I suspect Phil Oshel would greatly appreciate being able to give a home to Hayat's Fixation for Electron Microscopy.
Paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 22 -- From paul_hazelton-at-umanitoba.ca Sun Sep 7 20:18:33 2008 5, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m881IWkJ009143 5, 22 -- for {microscopy-at-microscopy.com} ; Sun, 7 Sep 2008 20:18:33 -0500 5, 22 -- Received: from [192.168.100.102] (wnpgmb014ww-ad06-73-192.dynamic.mts.net [207.161.73.192]) 5, 22 -- (authenticated bits=0) 5, 22 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m881IQQF025282 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 22 -- Sun, 7 Sep 2008 20:18:30 -0500 (CDT) 5, 22 -- Message-ID: {48C47D69.6070601-at-umanitoba.ca} 5, 22 -- Date: Sun, 07 Sep 2008 20:18:33 -0500 5, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 22 -- Organization: University of Manitoba 5, 22 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microbill-at-mohawk.net 5, 22 -- Subject: Re: [Microscopy] books 5, 22 -- References: {200809071802.m87I2nEv009988-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200809071802.m87I2nEv009988-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
I have set up an SEM facility in the high school where I work. We have a Cambridge S200 (almost works), and a nice S360 that does work. Books would come in handy. If they come with a free critical point drier and sputter coater even better :-)
-Mike
Dr. Mike Brown Science Dept Chair AAEC-PV MBrown-at-AAECHighschools.com
==============================Original Headers============================== 4, 19 -- From mbrown-at-aaechighschools.com Sun Sep 7 21:48:40 2008 4, 19 -- Received: from smtpoutwbe01.prod.mesa1.secureserver.net (smtpoutwbe01.prod.mesa1.secureserver.net [208.109.78.112]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m882mdj4023987 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Sun, 7 Sep 2008 21:48:39 -0500 4, 19 -- Received: (qmail 5100 invoked from network); 8 Sep 2008 02:48:36 -0000 4, 19 -- Received: from unknown (HELO gem-wbe21.prod.mesa1.secureserver.net) (64.202.189.205) 4, 19 -- by smtpoutwbe01.prod.mesa1.secureserver.net with SMTP; 8 Sep 2008 02:48:36 -0000 4, 19 -- Received: (qmail 7934 invoked by uid 99); 8 Sep 2008 02:48:27 -0000 4, 19 -- Content-Type: text/plain; charset="utf-8" 4, 19 -- X-Originating-IP: 75.172.234.136 4, 19 -- User-Agent: Web-Based Email 4.14.3 4, 19 -- Message-Id: {20080907194827.889a5134facffa13190a02200f773d01.b317d296b3.wbe-at-email.secureserver.net} 4, 19 -- From: mbrown-at-aaechighschools.com 4, 19 -- To: Microscopy-at-Microscopy.Com 4, 19 -- Subject: I have a nice home for your microscopy books 4, 19 -- Date: Sun, 07 Sep 2008 19:48:27 -0700 4, 19 -- Mime-Version: 1.0 4, 19 -- Content-Transfer-Encoding: 8bit 4, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m882mdj4023987 ==============================End of - Headers==============================
Congraduations on getting this set-up in your high school. I was able to get my local high school set up with a SEM a couple years ago. The high school teacher there I'm sure would be interested in curriculum ideas that you may have implimented. How will you maintain your instruments over time? That seems to be a major long term difficulty with this kind of expensive instrumentation.
dj
On Sun, 7 Sep 2008, mbrown-at-aaechighschools.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I have set up an SEM facility in the high school where I work. We have } a Cambridge S200 (almost works), and a nice S360 that does work. Books } would come in handy. If they come with a free critical point drier and } sputter coater even better :-) } } -Mike } } Dr. Mike Brown } Science Dept Chair } AAEC-PV } MBrown-at-AAECHighschools.com } } } } } ==============================Original Headers============================== } 4, 19 -- From mbrown-at-aaechighschools.com Sun Sep 7 21:48:40 2008 } 4, 19 -- Received: from smtpoutwbe01.prod.mesa1.secureserver.net (smtpoutwbe01.prod.mesa1.secureserver.net [208.109.78.112]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m882mdj4023987 } 4, 19 -- for {Microscopy-at-Microscopy.Com} ; Sun, 7 Sep 2008 21:48:39 -0500 } 4, 19 -- Received: (qmail 5100 invoked from network); 8 Sep 2008 02:48:36 -0000 } 4, 19 -- Received: from unknown (HELO gem-wbe21.prod.mesa1.secureserver.net) (64.202.189.205) } 4, 19 -- by smtpoutwbe01.prod.mesa1.secureserver.net with SMTP; 8 Sep 2008 02:48:36 -0000 } 4, 19 -- Received: (qmail 7934 invoked by uid 99); 8 Sep 2008 02:48:27 -0000 } 4, 19 -- Content-Type: text/plain; charset="utf-8" } 4, 19 -- X-Originating-IP: 75.172.234.136 } 4, 19 -- User-Agent: Web-Based Email 4.14.3 } 4, 19 -- Message-Id: {20080907194827.889a5134facffa13190a02200f773d01.b317d296b3.wbe-at-email.secureserver.net} } 4, 19 -- From: mbrown-at-aaechighschools.com } 4, 19 -- To: Microscopy-at-Microscopy.Com } 4, 19 -- Subject: I have a nice home for your microscopy books } 4, 19 -- Date: Sun, 07 Sep 2008 19:48:27 -0700 } 4, 19 -- Mime-Version: 1.0 } 4, 19 -- Content-Transfer-Encoding: 8bit } 4, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m882mdj4023987 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 21 -- From dljones-at-bestweb.net Sun Sep 7 22:03:39 2008 5, 21 -- Received: from mta2.srv.hcvlny.cv.net (mta2.srv.hcvlny.cv.net [167.206.4.197]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8833dxH004736 5, 21 -- for {microscopy-at-microscopy.com} ; Sun, 7 Sep 2008 22:03:39 -0500 5, 21 -- Received: from Pappee (ool-4352881b.dyn.optonline.net [67.82.136.27]) 5, 21 -- by mta2.srv.hcvlny.cv.net 5, 21 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 5, 21 -- with ESMTP id {0K6U001MHWI2N2B0-at-mta2.srv.hcvlny.cv.net} for 5, 21 -- microscopy-at-microscopy.com; Sun, 07 Sep 2008 23:03:39 -0400 (EDT) 5, 21 -- Date: Sun, 07 Sep 2008 23:03:45 -0400 (Eastern Daylight Time) 5, 21 -- From: "David L. Jones" {dljones-at-bestweb.net} 5, 21 -- Subject: Re: [Microscopy] I have a nice home for your microscopy books 5, 21 -- In-reply-to: {200809080251.m882pXEu029308-at-ns.microscopy.com} 5, 21 -- X-X-Sender: dljones-at-imap.bestweb.net 5, 21 -- To: mbrown-at-aaechighschools.com 5, 21 -- Cc: microscopy-at-microscopy.com 5, 21 -- Message-id: {Pine.WNT.4.64.0809072254380.5592-at-Pappee} 5, 21 -- MIME-version: 1.0 5, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 5, 21 -- Content-transfer-encoding: 7BIT 5, 21 -- References: {200809080251.m882pXEu029308-at-ns.microscopy.com} ==============================End of - Headers==============================
The response to my offer of books has somewhat overwhelmed me. Thanks to everyone who has responded. I will be getting back to each of you in order of your replies. This may take a few days, so please bear with me.
I have been made aware that I made a mistake with respect to the design of EA Fischione model 160 hand grinding tool and I would like to correct that mistake. I said that a hand grinder should not be turned upside down because water from the polishing could go into the threads of the tool. There is an O-ring in the design of the 160 that prevents the water from going into the threads to prevent this from happening. I apologize for the error. I've owned both the Gatan 623 and the Fischione 160 units and I assumed that I had no problems with corrosion because I never turned them upside down while wet and always dried them thoroughly when I was finished. I still think that this is good advice for any style of grinding/lapping tool.
I also was criticized for "touting" our lapping fixtures. I apologize if anyone thinks that was the case. As I have said, I've owned and extensively used all three brands of hand grinding tools that I mentioned during the span of my career. What I was trying to do is give advice on how to use each style and discuss the pro's and cons of the styles, without being commercial, which I thought I did. If I offended anyone, I humbly apologize.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: walck-at-SouthBayTech.com [mailto:walck-at-SouthBayTech.com] Sent: Friday, September 05, 2008 1:26 PM To: Walck-at-SouthBayTech.com
I've used the Model 623 and I am familiar with it, even though I now work for a competitor of Gatan.
Since you took it apart and the threads appear good, it sounds like the problem is not the polisher. If the threads are freely turning and they are not stripped, there is no physical way that they can make a linear jump to an extended position. But first, please check your stubs to make sure that they slide freely in and out of the hole and all the way to the bottom and that there is no excess wax on the sides of the stub. One problem that you can have with the Gatan 623 is that the SS stubs can slide too easily. This can be cured by scoring the sides of the stub with a sharp wire cutter. The Pyrex stubs generally don't have the same problem. If you have a dial or digital indicator, you can check the operation of the grinder by turning it upside down and checking the linear motion by turning the knob. Get a machinist or a mechanic to help you if you don't have the indicator tool. (Go to a brake shop and they would have the correct tools.) If that checks out, then there is nothing wrong with the grinder. Make sure that the knob is not slipping on the shaft when you turn it. Also, there is a push rod for pushing the stub out. Do not use the grinder with that in the hole. Take it out, you could be accidentally pushing the sample down while you use the fixture.
You probably need to supply some extra information. What is your sample? What are you using for abrasive? What are you using for lubrication? What are you trying to achieve -polished final sample or thinning for TEM? How are you affixing your samples to the stub, i.e. what type of wax are you using?
For hand grinding/lapping fixtures with micrometer control, there are two primary types. The first is the type that pushes the sample out as you turn the knob. The Gatan model 623 and the EA Fischione model 160 tools are examples of these. When you use these tools, the sample is pushed below the surface of the bottom of the fixture so that the fixture will actually wobble if the sample is pushed out to far. In this case, the whole weight of the fixture plus any downward force that you exert on the fixture, which you shouldn't do, is placed on the sample. When the sample is ground down until it is parallel to the bottom of the fixture, grinding of the sample stops. With this type of tool, the wax or glue that is used is under a lot of shear forces and it has to hold. In your case, you have to check that the wax is securely holding your sample. If you are dry grinding, the sample can get quite hot and that could soften the wax to change the working surface of your sample. If you are using a lubricant different than water, then make sure that it is not softening the wax. I also recommend that the fixture is not resting on the abrasive surface when you make your adjustment. You are statically pushing your sample into the abrasive which is not good.
The other type of fixture is the piston type exemplified by the South Bay Technology lapping fixtures, e.g. our model 150. In these, the sample is mounted on a block that is fastened to a piston that slides in the body of the fixture. The load on the sample is the weight of the piston, mounting block, and any additional weight that is put on top. These fixtures can be made to have a set of springs that offset the weight of the piston and mounting block for very light load polishing applications. The feet of the body establishes a polishing plane that does not rock and the piston/sample combinations falls as the sample is ground down until it comes to the limit set by the micrometer knob. These type of fixtures also can be used as the first type of fixtures above if the piston is locked in position with the sample set below the plane of the feet. These fixtures more readily lend themselves to automatic polishing stations compared to the first type because they can be set with a larger amount of material to be taken off and they don't have the rocking problem. Since the sample does not rock or have as high a load applied to it during the grinding these types of fixtures are less likely to have the problems with the high shear forces on the wax. One disadvantage of the piston type of fixtures is that hand grinding in the free piston mode is slower unless comparable weight is applied. Another disadvantage to these is that the piston could stick. Our fixtures have a dry lubricant coating on the piston. If the surface of that coating oxides because of the presence of moisture, it needs to be removed by simply wiping the piston with a dry cloth.
That reminds of a strict rule that novice users quite often forget -never turn a wet grinding/lapping fixture upside down. In the case of the first style (Gatan and Fischione) tools, the water can reach the threads and corrode them over time. In the case of ours, the solid film lubricant will oxidize and needs to be cleaned. Always dry the fixture while it is in the upright position before turning it upside down.
I'm not sure that I answered your question or not, but I thought that I would use the opportunity to discuss hand grinding and lapping. We have made a DVD that discusses various aspects of lapping. It features Earnest Apodoca, an expert in lapping, interacting with one of our customers to solve his lapping problems. It discusses the procedures for measuring and truing the lapping plate, truing the polishing feet and mounting blocks, how to measure flatness, zeroing the digital lapping fixture, obtaining a precise thickness, and controlling the damage depth during polishing. If you are interested in this lapping DVD, you can request a free copy. We make it available to people that are having problems lapping or who are considering doing demanding lapping.
Disclaimer: SBT makes and sells lapping fixtures.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: mcavaliere-at-mvainc.com [mailto:mcavaliere-at-mvainc.com] Sent: Thursday, September 04, 2008 6:17 PM To: Walck-at-SouthBayTech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mcavaliere-at-mvainc.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: We have a Gatan Model 623 Disc Grinder which does not appear to be advancing the sample correctly. Polishing seems to be going along smoothly and then all of a sudden with a turn of 5 microns or less the sample catches and is frequently pulled off the specimen mount and/or severely damaged. If the sample remains mounted, it is now sitting 10 to 100+ microns above the plane of the disc grinder! This seems to occur regardless of the grit of the paper, the RPMs of the polisher, and the sample material. We have taken the disc grinder apart and the threads do not look damaged. We have also replaced the o-ring and the spring. Any ideas on what might be going on and/or how to solve this?
Thanks,
Michelle Ritorto Cavaliere, Ph.D. Senior Research Scientist MVA Scientific Consultants, Inc. 3300 Breckinridge Blvd., Suite 400 Duluth, GA 30096 Phone: 770-662-8509 Fax: 770-662-8532
Jan, This really points out the problem. When your PC gets badly outdated you just shell out $1-2k and "upgrade" everything. Yes it's a PITA and seems expensive, but, of course, the newer software requires new hardware, but it should all work as a package.
The real problem comes with the fact that the non-PC part of the SEM is 98-99% of the cost. PCs become obsolete in a very short cycle (actually, obsolete when installed) and the customer is stuck replacing $100-500k worth of hardware (which we all know is good for a minimum of 20-30 years) when the PC can no longer be supported. It really can't even be passed on to a high school.
There HAS to be a better way!
I've had a number of customers ask me what they should buy for a new SEM to replace their old one. The first thing I ask them is what capabilities they need that they don't have. Often the answer is "none". "Then why do you want to replace a system that I can keep running forever with a system whose main controller will be obsolete before it's installed and will be unserviceable within 5 or so years?" "My boss told me to buy a new one because we got the capital budget for it this year."
A long, long time ago in a galaxy far away I worked for ETEC. One of their big selling points was that whenever they added new capabilities to the Autoscan, those capabilities could be added to any previous Autoscan. Sometimes it was more expensive on the earlier serial numbers, but mostly you just bought the option and added it. Users LOVED that. Many STILL love their Autoscans and many others deeply regret parting with one to make room for a new system with all the bells and whistles and attendant headaches, particularly software headaches.
I realize that you are caught in the middle. You really don't have a lot of options other than to go to PC control, but there are better and worse ways to do it. Amray built the control boards right into the PC. They absolutely require a 486 machine with an ISA buss. In the end, even THEY were having trouble supporting SEMS that were only a very few years old. Having a SCSI buss connection is a little better but that standard also changes pretty fast. Ethernet has been reasonably stable as has USB, but even there one is going to need to move to a newer network type long before your hardware is dead.
I'm no computer expert, but there has got to be a better way for PCs to talk to other (expensive) equipment that allows one to upgrade a PC with relative ease without having to trash all the good stuff.
I won't bother to bemoan what the PC has done to the independent service business, other than inspire me to keep older (read serviceable) SEMs from being taken out of service.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Jan.Ringnalda-at-fei.com [mailto:Jan.Ringnalda-at-fei.com] Sent: Friday, September 05, 2008 11:48 PM To: kenconverse-at-qualityimages.biz
Some intersting ideas, Gary and Gary.
I guess I will get on this soap box one more time...
If you have a system which is just hardware, and being controlled through a generic user interface, who would take responsibility for the system? I know we wouldn't...
We may go to Linux, after having already seen a number of VISTA tests fall flat on their faces. Who knows what tomorrow will bring? Maybe Macintosh is the way to go, or indeed Java?
X-from our point of view, it is the case that the microscope control system and the desktop OS is one aspect, the control system for the accessory manufacturers is another aspect. FEI, when they release an upgrade, release the Windos OS, the microsocpe control system and ALL accessory software that is needed, as one fully tested package; and as a result we are reasonably confident that this package will work properly in your system.
This is why we do not allow accessory manufacturers to update software on a Tecnai or Titan system, since they typically only test their own functionality, not the complete microscope system capabilities. So while to some people it may seem ludicrous that we are using accessory software that is three generations behind the current state of the art, watch these same people scream blue murder when the newest accessory SW is installed on their system, but now the microscope shutter no longer works, tomography doesn't function anymore and the low dose option has turned into the high dose option!
We have to test all of this before the final package is released, and this takes time. If an accessory manufacturer releases new SW to fix bugs, instead of fixing the existing SW with a patch or a service pack, this illustrates rushing to market without thorough testing for all applications. In such cases we have to do the testing for them. It is interesting that when we ask to get some imperfections or bugs fixed, the response is sometimes "we've already fixed that in the next version" but in that new version, all kinds of new bugs are present. That never leads to a stable system, and the testing becomes endless, with moving targets for the SW group; now THIS is a very frustrating experience.
All these things happen behind the closed doors of a company trying to make a system 'easy to use' and embed software from multiple vendors on to one platform. We still believe this is the best way to get systems at a higher uptime efficiency than those controlled by multiple vendors, but I agree it is a tough task to keep everyone happy. It has become clear, though, that a large number of users are not interested in the electron microscope, or the techniques, per-se, however everybody seems to be very interested in data. "Give me the data, I don't care how the system works" is a common theme. This poses a sizeable problem to us, since all users like the data to be correct.
I hope that by explaining this, it is clear that SW upgrades and OS upgrades cannot be done as part of the regular service contract, and that as a company we could not possibly survive if we did this free of charge. Some people ask for free SW upgrades; I would say if you really want a free upgrade, and you succeed to get a legitimate free upgrade of your OS from Microsoft, I will revisit this discussion.
On the other hand, if we get lots of good feedback about functionality, bugs, serviceability and/or capabilities of the system from some users, we would be much more inclined to encompass those users in beta-testing programs. We, as a company, need information about how systems are working in the field, both what is good as well as bad. Only if we get a good stream of information, can we make the right decisions about new SW directions and requirements. So keep the comments coming, they are being used... And to quote one of the Gary's: "Be careful what you wish for, you may just get it..."!
Jan
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I don't foresee the hardware ever being standardized. Each company has a different approach to how they get from the e source to specimen. I just think that the software platform will have to change. In this regard, the EM makers are at the mercy of the OS makers. For the most part, Microsoft. MS does not care what the impact is on their customers or even their niche EM customers. Just sell more OS units. Well, that halted with Vista. A junk OS. XP is stable and pretty well done...albeit with continuing security holes. Why go to Vista with a hostile interface and a whole new set of security holes? Time to move on.
It seems to me that each maker can make their own electronic and physical incarnation of an EM but use a standard software platform using Linux. The real kicker is if someone makes a Linux-based Perl or Java app for EM. That would narrow down the interface issue to a few key system calls. So now one would have hardware to buy and then separate software to buy to run the hardware.
The user interface could be nearly the same for different hardware makers. But this would not require major changes in the software interface. Plus, this would easily eliminate the stupid hardware dongles to protect their hardware specific software. This "protection" is just an additional cost that users pay. With a common software interface, the competitive advantage moves to what the hardware can do. There is clearly a lot of wiggle room in this regard.
gary g.
At 02:25 PM 9/5/2008, you wrote:
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} didn't have to babysit(analog) - hence , the computer controlled } electron microscope. It was and is a great idea, automating many } mundane tasks so one could concentrate their time on one's research. } And, the manufacturers responded en masse. They saw this era as one } that would reap very good, if not great financial corporate rewards. } Now, instead of an instrument lasting for 20, 30, even 40 years, being } maintained by the manufacturer, in-house staff, or third party } companies, this new, state of the art, PC based , Digital SEM/TEM(or } whatever) now only lasts for 5 to 10 years(maybe), because of some guy } named Moore(Moore's Law). They were/are now selling many times more } instruments/system than they did in the past, didn't have to worry } about keeping obsolete repair stock(for 20 years) and they cut out the } in-house/third party service organizations - another big money maker, } everything was sole sourced to the manufacturer, from the initial sale } to the on going service. Ching Ching! } Hardware development in all areas run at an extremely blistering } pace and when one designs to run a certain set of hardware with a } popular Operating System, it is essentially outdated when the } instrument comes to market(see Moore's Law). This is why seemingly simple and } inexpensive software upgrades are not possible. Also, the way that } the software "hooks" in the hardware changes radically for OS } generation to generation, making it that the more difficult. } It is EXTREMELY difficult if not impossible to re-engineer an } older(5 years) instrument, unless the customer wants to bear the full } brunt for re-engineering. In most cases, it is simply not worth it } financially. Because much of the "code" is locked in the silicon } itself, not one manufacturer's would be willing to give away the "keys } to the kingdom" to an interested third party and there isn't enough } sheer numbers of instruments to make it a viable(profitable) alternative. } There is no happy medium. If the hardware and software became } standardized, companies would lose any competitive edge in trying to } sell their own, individual unique product, the end of the free market } system(ha, just kidding!). So, it seems we all must follow the } technology "Pied Piper" into the future. It all just boils down to the
} dollars.......... } } "Be careful what you ask for, you just may get it...." } } } Gary Easton
==============================Original Headers============================== 26, 30 -- From Jan.Ringnalda-at-fei.com Fri Sep 5 22:45:09 2008 26, 30 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m863j9kE024796 26, 30 -- for {microscopy-at-microscopy.com} ; Fri, 5 Sep 2008 22:45:09 -0500 26, 30 -- X-WSS-ID: 0K6R93D-01-H03-01 26, 30 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 26, 30 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 2113199F6DB; 26, 30 -- Fri, 5 Sep 2008 20:45:13 -0700 (PDT) 26, 30 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 26, 30 -- Fri, 5 Sep 2008 20:45:08 -0700 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 30 -- Content-class: urn:content-classes:message 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Type: text/plain; 26, 30 -- charset="us-ascii" 26, 30 -- Subject: RE: [Microscopy] Re: FEI software - Another take 26, 30 -- Date: Fri, 5 Sep 2008 20:45:06 -0700 26, 30 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB813C9BF5D-at-hlexc03.w2k.feico.com} 26, 30 -- In-Reply-To: {200809060206.m8626eBN022280-at-ns.microscopy.com} 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- Thread-Topic: [Microscopy] Re: FEI software - Another take 26, 30 -- Thread-Index: AckPxTUU5HbU6eFAQDKdfsR2CKWIRAABQTMA 26, 30 -- References: {200809060206.m8626eBN022280-at-ns.microscopy.com} 26, 30 -- From: "Ringnalda, Jan" {Jan.Ringnalda-at-fei.com} 26, 30 -- To: {gary-at-gaugler.com} 26, 30 -- Cc: {microscopy-at-microscopy.com} 26, 30 -- X-OriginalArrivalTime: 06 Sep 2008 03:45:08.0289 (UTC) FILETIME=[F4E3D310:01C90FD2] 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m863j9kE024796 ==============================End of - Headers==============================
==============================Original Headers============================== 45, 25 -- From kenconverse-at-qualityimages.biz Mon Sep 8 14:01:03 2008 45, 25 -- Received: from mta13.adelphia.net (mta13.mail.adelphia.net [68.168.78.44]) 45, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m88J12h0019281 45, 25 -- for {microscopy-at-microscopy.com} ; Mon, 8 Sep 2008 14:01:02 -0500 45, 25 -- Received: from Ken ([72.227.100.25]) by mta13.adelphia.net 45, 25 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with ESMTP 45, 25 -- id {20080908185758.WLRL23173.mta13.adelphia.net-at-Ken} ; 45, 25 -- Mon, 8 Sep 2008 14:57:58 -0400 45, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 45, 25 -- To: {Jan.Ringnalda-at-fei.com} , "MSA Listserver" {microscopy-at-microscopy.com} 45, 25 -- Subject: RE: [Microscopy] FEI software - Another take 45, 25 -- Date: Mon, 8 Sep 2008 15:00:56 -0400 45, 25 -- Message-ID: {A5F6D69ED24D41E5ACC3F1D88CEFEFAA-at-Ken} 45, 25 -- MIME-Version: 1.0 45, 25 -- Content-Type: text/plain; 45, 25 -- charset="us-ascii" 45, 25 -- X-Priority: 3 (Normal) 45, 25 -- X-MSMail-Priority: Normal 45, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 45, 25 -- Thread-Index: AckP004fxnfxUhe4QdqqZDCJ7BeOaQCDFtfA 45, 25 -- Importance: Normal 45, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 45, 25 -- In-Reply-To: {200809060347.m863lbrB028821-at-ns.microscopy.com} 45, 25 -- Content-Transfer-Encoding: 8bit 45, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m88J12h0019281 ==============================End of - Headers==============================
} } I'm no computer expert, but there has got to be a better way for PCs to talk } to other (expensive) equipment that allows one to upgrade a PC with relative } ease without having to trash all the good stuff. }
I'll pipe in here now ;) There is a better way. Get the hardware out of the PC and stop using Microscoft specific features (COM/DCOM/DotNet, etc) It's migration cycle is too fast to be tied to expensive Microscope hardware/software and one does not have full control of the OS with todays GUI based operating system. Electron Microscope are expected to have a live cycle of at least 15 years. The standard computer platform life cycle is less than four years.
We took this approach with our RevolutionEDX software/hardware. The computer/OS is a commodity product that we don't care about it except that it is fast enough to run our software. We use a GigE interface to our hardware and use standard TCP/IP for communication, non of that Microsoft COM/DCOM or .Net crap. That way we can jump to different versions of OS or different OS platforms without having to worry about does the OS support this protocol. TCP/IP is a real network standard that will be around for many years and is very stable and all the flavors of OS support it. GigE not fast enough, then go 10 GigE or use industry standard methods to bind multiple GigE together.
We also do not use the windows registry for saving preferences. It's too volatile and a dead computer means a complete reinstall of everything. That's a real pain. We save our preferences with the application so you only need to make a copy of this folder and everything is saved. Reinstall is a simple as dragging a copy to a new computer. And it's a minimal impact on the OS regarding dependencies as we don't need specific versions of system libs to function.
Our hardware uses industry standard form factors for it's internal computer (ITX) and we don't care which ITX motherboard is used as long as it supports TCP/IP and USB. USB is used internally for comm to hardware cards. And it runs Linux because we can control every detail of the internal OS including exactly what programs are running.
As a result, we went from WinXP to Vista with no software changes and zero hardware changes. We can migrate RevolutionEDX in a WinXP system to a Vista system in about 10 mins.
Once you take the approach to get the hardware out of the GUI computer and use real industry standards (not Microsoft standards), then life becomes much easier. Yes, your software/hardware devs have to do a little more work but you gain much more back in the reduction of resources spent on tech support dealing with computer issues. FEI has taken the approach that the SEM computer is hands off for the customer, that's why there is the second computer for user apps and accessory like us EDX companies. While this does attempt to solve the computer software issues, it's not a real solution and adds complexity.
Scott
-- ----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 ext 13 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
==============================Original Headers============================== 11, 24 -- From davilla-at-4pi.com Mon Sep 8 14:43:36 2008 11, 24 -- Received: from mx.4pi.com (mx.4pi.com [24.172.19.59]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m88Jhakp001469 11, 24 -- for {microscopy-at-microscopy.com} ; Mon, 8 Sep 2008 14:43:36 -0500 11, 24 -- Received: from localhost (localhost [127.0.0.1]) 11, 24 -- by mx.4pi.com (Postfix) with ESMTP id 469EB3C69D98 11, 24 -- for {microscopy-at-microscopy.com} ; Mon, 8 Sep 2008 15:45:57 -0400 (EDT) 11, 24 -- X-Virus-Scanned: amavisd-new at 4pi.com 11, 24 -- Received: from mx.4pi.com ([24.172.19.59]) 11, 24 -- by localhost (orion.4pi.com [127.0.0.1]) (amavisd-new, port 10024) 11, 24 -- with ESMTP id JE2K7wFrKekI for {microscopy-at-microscopy.com} ; 11, 24 -- Mon, 8 Sep 2008 15:45:11 -0400 (EDT) 11, 24 -- Received: from [192.168.1.103] (rbl.4pi.com [24.172.19.62]) 11, 24 -- by mx.4pi.com (Postfix) with ESMTP id 1A2423C69D85 11, 24 -- for {microscopy-at-microscopy.com} ; Mon, 8 Sep 2008 15:45:11 -0400 (EDT) 11, 24 -- Mime-Version: 1.0 11, 24 -- Message-Id: {p06240806c4eb28676dfc-at-[192.168.1.103]} 11, 24 -- In-Reply-To: {200809081901.m88J18uf019330-at-ns.microscopy.com} 11, 24 -- References: {200809081901.m88J18uf019330-at-ns.microscopy.com} 11, 24 -- Date: Mon, 8 Sep 2008 15:42:31 -0400 11, 24 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 11, 24 -- From: "Scott D. Davilla" {davilla-at-4pi.com} 11, 24 -- Subject: [Microscopy] RE: FEI software - Another take 11, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
All, As a side bar I will just mention that in the EPMA world both vendors (Jeol and Cameca) have gone to network (TCP/IP) connections on their instruments and it's wonderful. This allows for all sorts of interesting possibilities such as having multiple computers talking to the instrument at the same time (hey, it's a network).
I even have had (for several years until I moved to a new building) my Cameca Sx100 running on the WWW so I could access it from home. That is, I was running control software on my home computer and talking directly to the instrument. I didn't have any hacker problems with the instrumemnt exposed on the web probably because it didn't look like a Windows box and was therefore ignored.
In fact I can run my instrument control software on a laptop and talk to the instrument just fine for acquisition and control. I've even developed multi-platform image acquisition software that runs on Windows, Linux and PCs. All possible because there is a standard network connection to the instrument.
Note that some EDS vendors such as Thermo have also gone this route because it's a portable and bullet proof way to communicate.
Ok, I see Scott has just chimed in and I agree with him. Let's go to standard TCP/IP connections for all scientific instruments. It provides the flexibility we all want. john
John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (lab) Eugene, OR 97403-1241
Scott & John, I knew there had to be something better! Now if we can get users to hold some feet to the fire, we can all stop throwing away our hard earned money because Bill Gates is unstable.
Thank you for the cogent replies.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] Sent: Monday, September 08, 2008 4:14 PM To: kenconverse-at-qualityimages.biz
All, As a side bar I will just mention that in the EPMA world both vendors (Jeol and Cameca) have gone to network (TCP/IP) connections on their instruments and it's wonderful. This allows for all sorts of interesting possibilities such as having multiple computers talking to the instrument at the same time (hey, it's a network).
I even have had (for several years until I moved to a new building) my Cameca Sx100 running on the WWW so I could access it from home. That is, I was running control software on my home computer and talking directly to the instrument. I didn't have any hacker problems with the instrumemnt exposed on the web probably because it didn't look like a Windows box and was therefore ignored.
In fact I can run my instrument control software on a laptop and talk to the instrument just fine for acquisition and control. I've even developed multi-platform image acquisition software that runs on Windows, Linux and PCs. All possible because there is a standard network connection to the instrument.
Note that some EDS vendors such as Thermo have also gone this route because it's a portable and bullet proof way to communicate.
Ok, I see Scott has just chimed in and I agree with him. Let's go to standard TCP/IP connections for all scientific instruments. It provides the flexibility we all want. john
John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (lab) Eugene, OR 97403-1241
Jan; As a matter of fact, I HAVE upgraded the software in my car. You see, engineers are by nature tinkerers. At least most of the good ones I know are. As for free upgrades, I believe Gatan offers free upgrades every six months for anyone licensed (they did pay for something!) to use to the software. I thought one of the attractions to a software based system was that it could be upgraded more easily than a hardware based system. I am presently using a 10-year old field emission TEM that has been upgraded so many times I can't recall them all, and it is now operating with I believe state of the art (for a non-aberration corrected microscope) capabilities. It would be impossible to go back to management and ask for a few million dollars every couple of years for a new TEM, and it really doesn't even make sense to consider that. If you go to MSA regularly, you realize that there are a lot of advances in microscopy techniques every year. Why should anyone be satisfied with doing things the old way when there are newer and better ways, and why should they have to wait for major funding if the improvements can be made with minor funding? I should add that the TEM I am using has the microscope controller running W2000, and the new CCD and new EELS systems running on a completely different computer under XP that communicates with the microscope through an Ethernet controller. The new EDX system also runs on a completely different XP computer that communicates with the microscope through an Ethernet controller. This is really pretty seamless and simple, and nothing in the CCD, EELS or EDX software will screw up anything the microscope controller has control over. As for your comment, "If it works, and it works to the level it was designed for, don't try to upgrade it.", I think you are expressing an attitude that does a great disservice to your customers. Microscopists are not really satisfied with the mediocre, and if we were not out trying to change the world, we would probably be in a different profession. I don't think anyone would suggest that microscope vendors continually upgrade capabilities for free for 10 years, but they need to be somewhat "open" so that upgrades can be added without screwing up the whole system, which seems to be a big concern of yours. Isn't this a lesson Apple took a long time to learn?
John Mardinly, Numonyx
-----Original Message----- X-from: Jan.Ringnalda-at-fei.com [mailto:Jan.Ringnalda-at-fei.com] Sent: Saturday, September 06, 2008 5:27 AM To: MARDINLY, A
All,
One of my statements is being mis-interpreted.
With reference to the 'free' microsoft OS upgrades, let me expand one statement:
FEI does provide as part of warranty or service contract, free service packs, patches and minor revision SW upgrades. This means going from verison 1 all the way through to 1.9 is included, these upgrades may include functionality enhancement, but typically does not include HW upgrades. Going from SW version 1.X to 2.0 would be chargeable, because this includes acquisition card upgrades if needed, PC hardware as well operating system OS upgrade, and usually includes significant functionality enhancements.
Thanks, Jan
==============================Original Headers============================== 6, 29 -- From Jan.Ringnalda-at-fei.com Sat Sep 6 07:21:11 2008 6, 29 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m86CLBMe004423 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 6 Sep 2008 07:21:11 -0500 6, 29 -- X-WSS-ID: 0K6RWZB-01-XGJ-01 6, 29 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 6, 29 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 2A3E887CFEF 6, 29 -- for {microscopy-at-microscopy.com} ; Sat, 6 Sep 2008 05:21:10 -0700 (PDT) 6, 29 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Sat, 6 Sep 2008 05:21:09 -0700 6, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 29 -- Content-class: urn:content-classes:message 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="us-ascii" 6, 29 -- Subject: FEI software - "Free Upgrades" 6, 29 -- Date: Sat, 6 Sep 2008 05:21:08 -0700 6, 29 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB813C9C058-at-hlexc03.w2k.feico.com} 6, 29 -- In-Reply-To: {200809060352.m863qR09005408-at-ns.microscopy.com} 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- Thread-Topic: FEI software - "Free Upgrades" 6, 29 -- Thread-Index: AckP0/3aGi7eQZjnR3GDHSPQyz8yFgARTzow 6, 29 -- References: {200809060352.m863qR09005408-at-ns.microscopy.com} 6, 29 -- From: "Ringnalda, Jan" {Jan.Ringnalda-at-fei.com} 6, 29 -- To: {microscopy-at-microscopy.com} 6, 29 -- X-OriginalArrivalTime: 06 Sep 2008 12:21:09.0874 (UTC) FILETIME=[0B6EC520:01C9101B] 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m86CLBMe004423 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From A.MARDINLY-at-numonyx.com Mon Sep 8 18:40:15 2008 17, 27 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m88NeEVH016296 17, 27 -- for {Microscopy-at-Microscopy.com} ; Mon, 8 Sep 2008 18:40:14 -0500 17, 27 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23]) 17, 27 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 9EABD1E8295 17, 27 -- for {Microscopy-at-Microscopy.com} ; Mon, 8 Sep 2008 17:44:26 -0400 (EDT) 17, 27 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 17, 27 -- Mon, 8 Sep 2008 19:40:14 -0400 17, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 27 -- Content-class: urn:content-classes:message 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="US-ASCII" 17, 27 -- Subject: RE: [Microscopy] FEI software - "Free Upgrades" 17, 27 -- Date: Mon, 8 Sep 2008 19:40:06 -0400 17, 27 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF984BCA6-at-EXDRESBENMX012.numonyx.local} 17, 27 -- X-MS-Has-Attach: 17, 27 -- X-MS-TNEF-Correlator: 17, 27 -- Thread-Topic: [Microscopy] FEI software - "Free Upgrades" 17, 27 -- Thread-Index: AckQG+io/iuhAlnAQOCZudtSRtYqVgBvjx+wAAHAYGAACnxvEA== 17, 27 -- References: {200809061226.m86CQkAH016626-at-ns.microscopy.com} 17, 27 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 17, 27 -- To: {Microscopy-at-Microscopy.com} 17, 27 -- X-OriginalArrivalTime: 08 Sep 2008 23:40:14.0013 (UTC) FILETIME=[3DA87ED0:01C9120C] 17, 27 -- Content-Transfer-Encoding: 8bit 17, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m88NeEVH016296 ==============================End of - Headers==============================
We need to hire a consultant to help us with an image analysis issue we're having with Adobe Photoshop CS3 Extended (primarily) and PAX-it. This is a short-term project - I doubt we'll need more than a day of consulting time. If anyone knows of an image analysis consultant who's an expert at Photoshop and is at least familiar with PAX-it, please send them my way. (E-mail is better than phones, at least initially, and it's possible we could do everything electronically, so the expert doesn't have to come on-site - but it could help)
Thanks to all,
Robert Zonis Product Development Chemist, LMTC Sanford L.P. - A Newell Rubbermaid Company 3 Sharpie Way Shelbyville, TN 37160 Direct: +1 (931) 685-6635
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I strongly recommend two people: A. Jerry Sedgewick. He has taught courses before with us, very successfully. You can reach him via email at Jerry Sedgewick |--sedge-at-USFAMILY.NET--|. Also, he has just published his most recent book on exactly this subject: Scientific Imaging with Photoshop: Methods, Measurement and Output (Publishers: New Riders, ISBN 0-321-51433-5).
B. John Russ. You can reach him via email at drjohnruss-at-aol.com. His book, "The Image Processing Handbook,2nd edition," (CRC Press, ISBN0-8493-2516-1) is a classic.
Good hunting! Barbara Foster, President
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now scheduling customized, on-site courses through Dec 2008. Call me for a free assessment and quote.
At 03:46 PM 9/9/2008, Robert.Zonis-at-Sanford.com wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I think you will find that John Russ is a better choice as he can not only show you how to measure but can guide you to more sophisticated techniques. Because he actually programs extensions to Photoshop and wrote a classic book, he will be able to solve any issues you might have.
John
-- John Mackenzie, Jr. Coordinator for the Center for Electron Microscopy Professor of Microbiology North Carolina State University Phone (919) 515-2664 Fax (919) 515-8293
As an invertebrate taxonomist I deal exclusively with light microscopy. Which one of these books (Sedgewick or Russ) is the useful and/or relevant to my needs?
Kelvin Barwick Oceanside Biology Laboratory San Francisco, CA
==============================Original Headers============================== 3, 25 -- From KBarwick-at-sfwater.org Wed Sep 10 09:45:44 2008 3, 25 -- Received: from mktscn01.ad1.sfwater.org (63-199-81-8.ded.pacbell.net [63.199.81.8] (may be forged)) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m8AEjift025111 3, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Sep 2008 09:45:44 -0500 3, 25 -- Received: From MKTSMTP01.ad1.sfwater.org ([10.40.200.48]) by mktscn01.ad1.sfwater.org (WebShield SMTP v4.5 MR2); 3, 25 -- id 1221057942817; Wed, 10 Sep 2008 07:45:42 -0700 3, 25 -- Received: from MLBEXC01.ad1.sfwater.org ([10.40.91.78]) by MKTSMTP01.ad1.sfwater.org with Microsoft SMTPSVC(6.0.3790.3959); 3, 25 -- Wed, 10 Sep 2008 07:45:42 -0700 3, 25 -- Content-class: urn:content-classes:message 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; 3, 25 -- charset="iso-8859-1" 3, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 25 -- Subject: Re: Image analysis 3, 25 -- Date: Wed, 10 Sep 2008 07:45:40 -0700 3, 25 -- Message-ID: {A8C94F712BCC8E4DB9B999EED31D1F85022C2E5A-at-MLBEXC01.ad1.sfwater.org} 3, 25 -- X-MS-Has-Attach: 3, 25 -- X-MS-TNEF-Correlator: 3, 25 -- Thread-Topic: Re: Image analysis 3, 25 -- thread-index: AckTU+UaYuPsELTBQQyeFSmTcS2UFQ== 3, 25 -- From: "Barwick, Kelvin" {KBarwick-at-sfwater.org} 3, 25 -- To: {Microscopy-at-microscopy.com} 3, 25 -- X-OriginalArrivalTime: 10 Sep 2008 14:45:42.0824 (UTC) FILETIME=[E690EA80:01C91353] 3, 25 -- Content-Transfer-Encoding: 8bit 3, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8AEjift025111 ==============================End of - Headers==============================
Dear colleagues Only two days are remaining for the registration for the 3d international workshop on Piezoresponse Force Microscopy, held in conjunction with The Center for Nanophase Materials Science User meeting. With ~50 attendees (and counting), the workshop will offer an exciting program featuring latest developments in the field of PFM, nanoferroelectrics, and electromechanics in biological systems. Additionally, the workshop will feature mini-symposium on PFM spectroscopy and presentations by leading SPM manufacturers on PFM, Kelvin probe microscopy, novel dynamics SPM modes, and nanoscale thermal analysis. The workshop and registration information are available at www.cnms.ornl.gov Yours Sergei V. Kalinin
==============================Original Headers============================== 1, 25 -- From sergei2-at-ornl.gov Wed Sep 10 11:51:31 2008 1, 25 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 1, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8AGpVbm027067 1, 25 -- for {microscopy-at-microscopy.com} ; Wed, 10 Sep 2008 11:51:31 -0500 1, 25 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 1, 25 -- by emroute3.ornl.gov (PMDF V6.4 #31561) 1, 25 -- with ESMTP id {0K6Z00HEEO5TEX-at-emroute3.ornl.gov} for 1, 25 -- microscopy-at-microscopy.com; Wed, 10 Sep 2008 12:51:29 -0400 (EDT) 1, 25 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 1, 25 -- (PMDF V6.4 #31561) id {0K6Z00I01O5SBL-at-emroute3.ornl.gov} for 1, 25 -- microscopy-at-microscopy.com; Wed, 10 Sep 2008 12:51:28 -0400 (EDT) 1, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 1, 25 -- by emroute3.ornl.gov (PMDF V6.4 #31561) 1, 25 -- with ESMTP id {0K6Z00HGWO5S6C-at-emroute3.ornl.gov} for 1, 25 -- microscopy-at-microscopy.com; Wed, 10 Sep 2008 12:51:28 -0400 (EDT) 1, 25 -- Date: Wed, 10 Sep 2008 12:51:28 -0400 1, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 1, 25 -- Subject: PFM Workshop/CNMS User meeting 1, 25 -- To: microscopy-at-microscopy.com 1, 25 -- Cc: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 1, 25 -- Message-id: {48C7FB10.1030304-at-ornl.gov} 1, 25 -- MIME-version: 1.0 1, 25 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 1, 25 -- Content-transfer-encoding: 7bit 1, 25 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) ==============================End of - Headers==============================
Hi all, What pH should the coolant be in an EM chillers/water recirculator?
thanks, Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
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On Sep 10, 2008, at 3:14 PM, beth-at-plantbio.uga.edu wrote:
} What pH should the coolant be in an EM chillers/water recirculator?
Dear Beth, Slightly basic, say ~8.5, so as not to dissolve the copper in the fittings. The water will dissolve CO2 from the air over time, so if you adjust the pH to 8.5, it should be sufficiently basic for } 1 mo. I suggest checking every month and adjusting pH, anti-corrosion agent (if any), and removing any crud in the chiller tank. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Sep 10 18:12:29 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8ANCTEj020791 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 10 Sep 2008 18:12:29 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 465A566E07FD 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 10 Sep 2008 16:12:29 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 519BB66E013F 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 10 Sep 2008 16:12:28 -0700 (PDT) 6, 22 -- Message-Id: {ADDF502F-3F49-4C95-82B3-0942AD6510D9-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200809102214.m8AMEGpw032345-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v926) 6, 22 -- Subject: Re: [Microscopy] recirculating chillers coolant pH 6, 22 -- Date: Wed, 10 Sep 2008 16:12:27 -0700 6, 22 -- References: {200809102214.m8AMEGpw032345-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.926) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both monica.nelea-at-polymtl.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hello, I am using an ESEM Quanta 200 FEG miscroscope from FEI for analyzing samples for industry under good laboratory practice (GLP) regulations. Our industrial partner asked us to perform an instrument validation action. Does somebody may know how to perform or what it means the validation of a SEM? Many thanks for any helpful suggestion. Regards, Monica
When the laboratory faces the type of question you have it usually means the following:-
1. Do you follow recognised operating procedures, those that are set down in writing?
2. Do you calibrate your machine regularly with a recognised standard? Do you follow recognised operating procedures, those that are set down in writing?
3. If you are providing analytical information do you run analytical standards to check your accuracy? Do you follow recognised operating procedures, those that are set down in writing?
4. What are the qualifications of those carrying out the consultancy and are they to a standard that is adequate to carry out the investigations?
In short they are asking to what level you are competent to operate and do you have accreditation form a recognised body?
Check out the body or bodies in your country that certify competence? There is much to explain so check out our web site for more on Quality in Electron Microscopy.
Hope this helps but come back privately if you need more information so that I do not feel the wrath of Nester :)
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: monica.nelea-at-polymtl.ca [mailto:monica.nelea-at-polymtl.ca] Sent: 11 September 2008 02:22 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both monica.nelea-at-polymtl.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hello, I am using an ESEM Quanta 200 FEG miscroscope from FEI for analyzing samples for industry under good laboratory practice (GLP) regulations. Our industrial partner asked us to perform an instrument validation action. Does somebody may know how to perform or what it means the validation of a SEM? Many thanks for any helpful suggestion. Regards, Monica
My question is prompted by the inquiry about ESEM Validation from Monica Nelea and the reply by Steve Chapman. In the context of FDA (US Food and Drug Administration) regulations, how can one verify that a laboratory offering non-clinical testing services is indeed compliant with FDA cGLP (current good laboratory practices)? What agencies assist a lab in demonstrating compliance?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 3, 24 -- From donc-at-asmicro.com Wed Sep 10 22:49:01 2008 3, 24 -- Received: from smtp119.sbc.mail.re3.yahoo.com (smtp119.sbc.mail.re3.yahoo.com [66.196.96.92]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m8B3n0hq006056 3, 24 -- for {microscopy-at-microscopy.com} ; Wed, 10 Sep 2008 22:49:00 -0500 3, 24 -- Received: (qmail 65512 invoked from network); 11 Sep 2008 03:49:00 -0000 3, 24 -- Received: from unknown (HELO asm15) (asmicro-at-sbcglobal.net-at-68.51.122.238 with login) 3, 24 -- by smtp119.sbc.mail.re3.yahoo.com with SMTP; 11 Sep 2008 03:49:00 -0000 3, 24 -- X-YMail-OSG: wtm6qe0VM1kmTr5iOlp4.HnUJ1dZoEwt6CyJPPGQHOUyi5bWKmnmmHCIo1brUDP2rJsbwiKFTDkGM8VskFt0.71NDx5K01.nlWWCFxkrGGs_6kuJofkXHLJcWkE7jch7ExUfM_gSd1euPJO83iOsbP8B 3, 24 -- X-Yahoo-Newman-Property: ymail-3 3, 24 -- Message-ID: {C7BC415B08374444B71FD486544192D9-at-asm15} 3, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 3, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 3, 24 -- Subject: Validation: cGLP and FDA 3, 24 -- Date: Wed, 10 Sep 2008 23:48:21 -0400 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- format=flowed; 3, 24 -- charset="iso-8859-1"; 3, 24 -- reply-type=original 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Believe me Jan, we already discussed about this in the list and we are all aware of it. It is a tendency that most of us noticed. This is the dark side of the "easy-to-use", "computer-assisted" new generations of complex instruments like electron microscopes. Getting results is easy. Getting meaningful results is another question ;-)
Regards, Stephane
} It has become } clear, though, that a large number of users are not interested in the } electron microscope, or the techniques, per-se, however everybody seems } to be very interested in data. "Give me the data, I don't care how the } system works" is a common theme. This poses a sizeable problem to us, } since all users like the data to be correct.
==============================Original Headers============================== 7, 22 -- From nizets2-at-yahoo.com Thu Sep 11 03:54:01 2008 7, 22 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m8B8s1mP031090 7, 22 -- for {microscopy-at-microscopy.com} ; Thu, 11 Sep 2008 03:54:01 -0500 7, 22 -- Received: (qmail 60087 invoked by uid 60001); 11 Sep 2008 08:54:00 -0000 7, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 22 -- s=s1024; d=yahoo.com; 7, 22 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 22 -- b=XEpnFhlUESIwRQls4Y2Mm82MetSGS52A8BtTB3lXe64OVn455brMrh6j1fjZYhBWWliH+59Nf5U2pmtJ27IggSFq8aUoRpmOZ0zZ0CJvBWNNqW5X/GpavhUTsmNeLFP+OS8wlnwR9ZrUFafq/MDi4cMKTfi/WgU6BzylyFFASZk=; 7, 22 -- X-YMail-OSG: gO1g9eMVM1mGS9BD.NDTO4tsdOHV3S6m04wh2HdoqrREtpO_rzOiraYMTT9xbUnWodQl3tqthgVrjypVkqNpQ3EiQp29LHfcO7jlYnkcz7Eex8IgFgv9K5HbkXfTCCkpKts- 7, 22 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Thu, 11 Sep 2008 01:54:00 PDT 7, 22 -- X-Mailer: YahooMailRC/1096.28 YahooMailWebService/0.7.218.2 7, 22 -- Date: Thu, 11 Sep 2008 01:54:00 -0700 (PDT) 7, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 22 -- Subject: Re: [Microscopy] FEI software - Another take 7, 22 -- To: Jan.Ringnalda-at-fei.com 7, 22 -- Cc: microscopy-at-microscopy.com 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=iso-8859-1 7, 22 -- Message-ID: {739962.58979.qm-at-web37406.mail.mud.yahoo.com} 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8B8s1mP031090 ==============================End of - Headers==============================
I delayed for a while the unpleasant duty of writing a reply to your message, but finally it will be done. You badmouthed not only my paper, but also the good name of a good (if not the best for electron microscopists) magazine 'Microscopy Today'. You wrote: "An article entitled 'Combining Pictures for Electron Microscopists' appears in the July 2008 issue of Microscopy Today. Unfortunately, as was the case for a previous series of articles printed in this magazine purporting to show how to use Photoshop, the procedure shown and the explanation of the steps is entirely wrong." So, we got The One and Only True Way to Use Photoshop. What's next? The One and Only True Recipe for an Apple Pie? And, by the way, why did you replace the title of my paper from 'Coloring pictures for electron microscopists' to the dull 'Combining pictures...?"'
As was rightfully mentioned by Dr. Mackenzie, you suggested an alternative (but not the only) method for coloring pictures using channels instead of layers. Now I see it was my mistake not to mention this method in my paper; and it is a bit faster and can save up to 2 minutes per picture. But I used layers, not channels, because in my opinion layers provide more freedoms in tweaking pictures for obtaining artistic results that are satisfying for an electron microscopist who is coloring pictures (Dr. Russ, please remember, my paper is titled 'Coloring pictures', not 'Combining pictures'.) For layers we can change Fill Opacity, Blend Mode, use layers not just as R, G, B channels, but, for example, as R, G, B+R channels, etc.
Dr. Russ, nowadays it is not a problem to find an expert in Photoshop among professional photographers, artists, and even students (young people can be much better with software then us, the older microscopists.) If you ask these experts about The One and Only True Way to Use Photoshop, you'll make them laugh. Photoshop was made not to make people to obey rules, but to assist them in their creativity.
Sincerely,
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} } A recent article in Microscopy Today showed a method for } merging multiple images to form a color composite, using } Photoshop. I discussed with John Russ his alternative method } which offers an easy way to perform this alignment of the } images. He has created a pdf file that can be downloaded } from {www.drjohnruss.com/downloads/merge.pdf} " This } illustrates with screen snapshots a method that anyone who is } interested should download and compare. } } John } } -- } John M. Mackenzie, Jr., PhD } Professor of Microbiology } Coordinator, Center for Electron Microscopy North Carolina } State University } Phone (919) 515-2664 Fax (919) 515-8293 } } } } ==============================Original } Headers============================== } 5, 17 -- From john_mackenzie-at-ncsu.edu Sun Aug 24 17:14:31 2008 } 5, 17 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.122]) } 5, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id m7OMEVE5005491 } 5, 17 -- for {Microscopy-at-microscopy.com} ; Sun, 24 Aug } 2008 17:14:31 -0500 } 5, 17 -- Received: from [127.0.0.1] (really [66.57.53.232]) } 5, 17 -- by cdptpa-omta04.mail.rr.com with ESMTP } 5, 17 -- id } {20080824221431.PCCO13299.cdptpa-omta04.mail.rr.com-at-[127.0.0.1]} ; } 5, 17 -- Sun, 24 Aug 2008 22:14:31 +0000 } 5, 17 -- Message-ID: {48B1DCC1.1010902-at-ncsu.edu} } 5, 17 -- Date: Sun, 24 Aug 2008 18:12:17 -0400 } 5, 17 -- From: John Mackenzie {john_mackenzie-at-ncsu.edu} } 5, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) } 5, 17 -- MIME-Version: 1.0 } 5, 17 -- To: Microscopy-at-microscopy.com } 5, 17 -- Subject: Re: Image Merging: An alternative method } 5, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 17 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 12, 29 -- From DusevichV-at-umkc.edu Thu Sep 11 08:35:49 2008 12, 29 -- Received: from kc-msxproto3.kc.umkc.edu (kc-msxproto3.kc.umkc.edu [134.193.44.10]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8BDZmWP003098 12, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Sep 2008 08:35:49 -0500 12, 29 -- X-Received-From-Address: 134.193.32.11 12, 29 -- X-Envelope-To: Microscopy-at-microscopy.com 12, 29 -- X-Envelope-From: DusevichV-at-umkc.edu 12, 29 -- Received: from KC-MSX1.kc.umkc.edu ([134.193.32.11]) by kc-msxproto3.kc.umkc.edu with Microsoft SMTPSVC(6.0.3790.3959); Thu, 11 Sep 2008 08:35:48 -0500 12, 29 -- Importance: normal 12, 29 -- Priority: normal 12, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4325 12, 29 -- Content-Class: urn:content-classes:message 12, 29 -- MIME-Version: 1.0 12, 29 -- Content-Type: text/plain; 12, 29 -- charset="us-ascii" 12, 29 -- Subject: RE: [Microscopy] Re: Image Merging: An alternative method 12, 29 -- Date: Thu, 11 Sep 2008 08:35:48 -0500 12, 29 -- Message-ID: {032EC4F75A527A4FA58C5B1B5DECFBB3062CB748-at-KC-MSX1.kc.umkc.edu} 12, 29 -- In-Reply-To: {200808242215.m7OMFL2a006373-at-ns.microscopy.com} 12, 29 -- X-MS-Has-Attach: 12, 29 -- X-MS-TNEF-Correlator: 12, 29 -- Thread-Topic: [Microscopy] Re: Image Merging: An alternative method 12, 29 -- thread-index: AckGOGQbkadwmoCuSiqLfSbNADGSxQN2sPyg 12, 29 -- References: {200808242215.m7OMFL2a006373-at-ns.microscopy.com} 12, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 12, 29 -- To: {Microscopy-at-microscopy.com} 12, 29 -- X-OriginalArrivalTime: 11 Sep 2008 13:35:48.0574 (UTC) FILETIME=[4D02E7E0:01C91413] 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8BDZmWP003098 ==============================End of - Headers==============================
Dear colleagues, Just over a day remains for registration for the Workshop on Aberration-Corrected Microscopy and Spectroscopy of Materials, to be held at the Oak Ridge National Laboratory on Sept 22-23 as a part of CNMS/ShaRE users week. With 25 attendees currently registered, the workshop has an impressive list of internationally recognized invited speakers: M. Watanabe (Lawrence Berkeley National Laboratory), R. Klie (U. of Illinois at Chicago), L. Houben (FZ Juelich), P.Voyles (U. of Wisconsin - Madison), M. Walls (U. Paris-Sud), O.Lourie (Nanofactory Instruments AB). The tutorial program, designed to cover most aspects of the use of aberration-corrected microscopy for materials, will be presented by Oak Ridge researchers: A. Lupini, M. Oxley, A. Borisevich, L. Allard, M. Varela. In addition to tutorials and invited talks, demonstrations will be held on several of ORNL's advanced microscopes, illustrating high-resolution STEM imaging, EELS spectroscopy, and advanced /in situ/ holders. Registration is available online at https://www.ornl.gov/cnms/share/ . Please don't forget to check the tab for this workshop when registering! We are looking forward to seeing you at Oak Ridge. Yours, Albina Y. Borisevich
==============================Original Headers============================== 2, 25 -- From albinab-at-ornl.gov Thu Sep 11 11:28:32 2008 2, 25 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 2, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8BGSWih031576 2, 25 -- for {microscopy-at-microscopy.com} ; Thu, 11 Sep 2008 11:28:32 -0500 2, 25 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 2, 25 -- by emroute3.ornl.gov (PMDF V6.4 #31561) 2, 25 -- with ESMTP id {0K7100IBHHRJN4-at-emroute3.ornl.gov} for 2, 25 -- microscopy-at-microscopy.com; Thu, 11 Sep 2008 12:28:31 -0400 (EDT) 2, 25 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 2, 25 -- (PMDF V6.4 #31561) id {0K7100J01HRJDH-at-emroute3.ornl.gov} for 2, 25 -- microscopy-at-microscopy.com; Thu, 11 Sep 2008 12:28:31 -0400 (EDT) 2, 25 -- Received: from [160.91.48.156] (simulations1.ornl.gov [160.91.48.156]) 2, 25 -- by emroute3.ornl.gov (PMDF V6.4 #31561) 2, 25 -- with ESMTPSA id {0K7100I99HRIW2-at-emroute3.ornl.gov} for 2, 25 -- microscopy-at-microscopy.com; Thu, 11 Sep 2008 12:28:31 -0400 (EDT) 2, 25 -- Date: Thu, 11 Sep 2008 12:28:30 -0400 2, 25 -- From: Albina Borisevich {albinab-at-ornl.gov} 2, 25 -- Subject: CNMS/ShaRE workshop on Aberration-Corrected Microscopy and 2, 25 -- Spectroscopy for Materials: last call 2, 25 -- To: microscopy-at-microscopy.com 2, 25 -- Message-id: {48C9472E.1020705-at-ornl.gov} 2, 25 -- MIME-version: 1.0 2, 25 -- Content-type: text/plain; format=flowed; charset=UTF-8 2, 25 -- Content-transfer-encoding: 7bit 2, 25 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) ==============================End of - Headers==============================
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Email: yngli-at-ucdavis.edu Name: YING LI
Title-Subject: [Filtered] How to get EFTEM with JEOL JEM 2500 SE TEM
Question: Hi, all, There anybody know the procedure for doing EFTEM imgae(energy filter transmission electron microscopy) with JEOL JEM 2500 SE TEM? That also named as EELS mapping, if you have used JEM 2500 SE before, and also know the procedures to get EFTEM, can you just tell me the basic steps of it? Thanks a lot!
Best regards Ying Li(Y. Li) ------------------------------------------------------------------------------------------------------------ Department of Chemical Engineering and Materials Science University of California, Davis, One Shields Avenue,Davis, CA 95616 Phone:530-752-9568; email:yngli-at-ucdavis.edu
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Email: richard.doelle-at-arcelormittal.com Name: Richard Doelle
Organization: ArcelorMittal Dofasco
Title-Subject: [Filtered] Service Providers for Oxford Inca EDS System in Toronto, Canada Area
Question: Hello Everyone,
Can anyone give me information regarding service providers, other than Oxford, for an Inca EDS system attached to an SEM?
This is in the Hamilton area (near Toronto) in Canada.
Preferably, the provider can issue a service contract. However I would also be interested in a provider who can be called upon for as-required service only.
Thanks.
Richard Doelle Senior Technology Specialist ArcelorMittal Dofasco
Research and Development Box 2460, 1390 Burlington St. E. Hamilton, Ontario L8N 3J5, Canada
Listers, I had the wrong email for Jay. The one below is correct.
I had a call from an individual who is looking for someone to do microscopy service work, preferably from a lab in the central Florida area, although this is not a requirement.
The individual wants to obtain micrographs of fine scratches and wear patterns on metal components. He is not familiar with microscopy and was not able to tell me if the patterns he wanted images of required SEM, or whether light optical microscopy was sufficient. He said that the subject patterns were not visible to the naked eye.
His name is Jay Zembower and can be reached at cardoctor1-at-hotmail.com
Please correspond directly with Jay without copying me or the listserver.
Thank you,
Ron Anderson
==============================Original Headers============================== 10, 17 -- From randerson20-at-tampabay.rr.com Fri Sep 12 13:32:18 2008 10, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.124]) 10, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8CIWI3W025766 10, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 12 Sep 2008 13:32:18 -0500 10, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 10, 17 -- by hrndva-omta05.mail.rr.com with ESMTP 10, 17 -- id {20080912183217.PZYQ17523.hrndva-omta05.mail.rr.com-at-[127.0.0.1]} 10, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 12 Sep 2008 18:32:17 +0000 10, 17 -- Message-ID: {48CAB5A8.2040200-at-tampabay.rr.com} 10, 17 -- Date: Fri, 12 Sep 2008 14:32:08 -0400 10, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 10, 17 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 10, 17 -- MIME-Version: 1.0 10, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 10, 17 -- Subject: Microscopy Services needed CORRECTION 10, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jtetlow1-at-rochester.rr.com Name: Jim Tetlow
Organization: Eternal Productions
Title-Subject: [Filtered] Video footage of Bacteria
Question: I am searching for video footage of bacteria. In particular, time-lapse footage showing bacteria multiplying as well as close-up footage showing the flagellum.
There are lots of good bacterial movies (FUN and high quality) at Julie Theriot's site http://cmgm.stanford.edu/theriot/movies.htm
David
On Sep 12, 2008, at 1:51 PM, jtetlow1-at-rochester.rr.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both jtetlow1-at-rochester.rr.com as well as the } MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: jtetlow1-at-rochester.rr.com } Name: Jim Tetlow } } Organization: Eternal Productions } } Title-Subject: [Filtered] Video footage of Bacteria } } Question: I am searching for video footage of bacteria. In } particular, time-lapse footage showing bacteria multiplying as well } as close-up footage showing the flagellum. } } Can anyone direct me to a good source? } } Thanks, } Jim } } Login Host: 72.230.145.245 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Fri Sep 12 15:48:06 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m8CKm5hT015217 } 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 12 Sep 2008 } 15:48:05 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c4f085f32bd8-at-[206.69.208.22]} } 8, 11 -- Date: Fri, 12 Sep 2008 15:48:04 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: jtetlow1-at-rochester.rr.com (by way of } MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Video footage of Bacteria } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 22 -- From Elliott-at-arizona.edu Fri Sep 12 19:13:45 2008 6, 22 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8D0DjCh004996 6, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Fri, 12 Sep 2008 19:13:45 -0500 6, 22 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 6, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 696A568FF28 6, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Fri, 12 Sep 2008 17:13:44 -0700 (MST) 6, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 6, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 8B66B68CE73 6, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Fri, 12 Sep 2008 17:13:43 -0700 (MST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 22 -- In-Reply-To: {200809122051.m8CKp5WX019723-at-ns.microscopy.com} 6, 22 -- References: {200809122051.m8CKp5WX019723-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {B4370C24-7C45-493A-B76D-6324D62F4E80-at-arizona.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: David Elliott {Elliott-at-arizona.edu} 6, 22 -- Subject: Re: [Microscopy] viaWWW: Video footage of Bacteria 6, 22 -- Date: Fri, 12 Sep 2008 17:13:39 -0700 6, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.753.1) 6, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Dear MSA List, Bev, who took the pictures of the Recipients of the Presidential Award, please contact me off line. Thanks. Jeannette
-- Jeannette Taylor, Technologist II Robert P. Apkarian Integrated Electron Microscopy Core Cherry L. Emerson Hall, Room E106 Emory University 1515 Dickey Drive Atlanta, Georgia 30322
We accepted a study for demonstrating viruses with negative staining in stool samples in TEM. Could you please share your experiences especially on simplest methods, so we may use the method(s) for routine examination later?. By the way we have no ultracentrifuge... Thanks in advance
Necat Yilmaz
==============================Original Headers============================== 4, 31 -- From nyilmaz-at-mersin.edu.tr Mon Sep 15 15:42:45 2008 4, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3]) 4, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8FKgisl019474 4, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Sep 2008 15:42:45 -0500 4, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id E02DD3252EC 4, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Sep 2008 23:41:46 +0300 (EEST) 4, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 4, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 4, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 4, 31 -- with ESMTP id QaO42xPotSsB for {Microscopy-at-microscopy.com} ; 4, 31 -- Mon, 15 Sep 2008 23:41:17 +0300 (EEST) 4, 31 -- Received: from nejat1 (unknown [78.167.38.176]) 4, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 9036232528A 4, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Sep 2008 23:41:17 +0300 (EEST) 4, 31 -- Message-ID: {000501c91773$8c6384e0$2101a8c0-at-nejat1} 4, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 4, 31 -- Subject: Negative virus staining 4, 31 -- Date: Mon, 15 Sep 2008 23:42:19 +0300 4, 31 -- MIME-Version: 1.0 4, 31 -- Content-Type: text/plain; 4, 31 -- format=flowed; 4, 31 -- charset="iso-8859-9"; 4, 31 -- reply-type=original 4, 31 -- Content-Transfer-Encoding: 7bit 4, 31 -- X-Priority: 3 4, 31 -- X-MSMail-Priority: Normal 4, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 4, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
We just had a Cambridge S360 donated to our high school. It was working briefly, but then failed. After replacing a contactor block in the power supply and the bridge rectifier on the vacuum board it now works again but...
I never get the "Gun vac ready" message. I Get "Gun Vac Not Available". This despite the fact I can pump the vacuum down to 1.7E-7 torr.
If I try and auto run I get the following error codes:
Message 305/2 filament current is off -hardware fault Message 305 filament current is off -hardware fault Message 305/1 EHT is off -hardware fault
Any suggestions as to what the issue might be would be greatly appreciated.
Mike
-Dr. Mike Brown Science Dept Chair AAEC-PV
==============================Original Headers============================== 10, 19 -- From mbrown-at-aaechighschools.com Mon Sep 15 18:46:51 2008 10, 19 -- Received: from smtpoutwbe11.prod.mesa1.secureserver.net (smtpoutwbe11.prod.mesa1.secureserver.net [208.109.78.27]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m8FNkoGf007208 10, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 15 Sep 2008 18:46:50 -0500 10, 19 -- Received: (qmail 13851 invoked from network); 15 Sep 2008 23:46:50 -0000 10, 19 -- Received: from unknown (HELO gem-wbe13.prod.mesa1.secureserver.net) (64.202.189.100) 10, 19 -- by smtpoutwbe11.prod.mesa1.secureserver.net with SMTP; 15 Sep 2008 23:46:50 -0000 10, 19 -- Received: (qmail 17608 invoked by uid 99); 15 Sep 2008 23:46:49 -0000 10, 19 -- Content-Type: text/plain; charset="utf-8" 10, 19 -- X-Originating-IP: 207.225.185.51 10, 19 -- User-Agent: Web-Based Email 4.14.3 10, 19 -- Message-Id: {20080915164649.889a5134facffa13190a02200f773d01.8fb6eea176.wbe-at-email.secureserver.net} 10, 19 -- From: mbrown-at-aaechighschools.com 10, 19 -- To: Microscopy-at-Microscopy.Com 10, 19 -- Subject: Gun vac not available issue with Cambridge S360 10, 19 -- Date: Mon, 15 Sep 2008 16:46:49 -0700 10, 19 -- Mime-Version: 1.0 10, 19 -- Content-Transfer-Encoding: 8bit 10, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8FNkoGf007208 ==============================End of - Headers==============================
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Title-Subject: [Filtered] searching for photo documentation
Question: Hi,
I am a student at Oslo National Academy of the Arts. I am looking for pictures/photos for my assignment about the death penalty in Usa. What i am looking for is the micro chemical grafic®identification® of the 3 drugs used in lethal injection:
I want to know if it is possible to find pictures using polarizing microscope or other types of tecnique that illustrates the chemicals in crystal, or in other forms that shows the caracteristic for these drugs?
Naval Research Laboratory is looking for an electron microscopist to run its transmission electron microscopy facility at NRL Marine Geosciences Division at Stennis Space Center, Mississippi. The facility houses JEOL JEM-3010 TEM with Environmental Cell, Noran Energy-dispersive X-ray Spectroscopy (EDXS), and Gatan Image Filter Model GIF 200. In addition, there are Hitachi H-600 TEM and sample preparation facility (http://www7430.nrlssc.navy.mil/facilities/emf/index.htm). Please contact Dr. Yoko Furukawa (228 688 5474, yoko.furukawa-at-nrlssc.navy.mil) for further information.
---------- Yoko Furukawa, PhD, Geochemist Naval Research Laboratory Code 7431 Stennis Space Center, MS 39529 USA (228) 688-5474
==============================Original Headers============================== 3, 23 -- From yoko.furukawa-at-nrlssc.navy.mil Wed Sep 17 07:13:01 2008 3, 23 -- Received: from mail.nrlssc.navy.mil (mail1.nrlssc.navy.mil [128.160.35.1]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8HCD1E0007784 3, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 17 Sep 2008 07:13:01 -0500 3, 23 -- Received: by mail.nrlssc.navy.mil id m8HCD0BJ003897; Wed, 17 Sep 2008 07:13:00 -0500 3, 23 -- Message-Id: {vymDT0kvPb1I2vmuWPNComqgOoQtMcp4Qr_gnFELw1dQwDLhT9M7MVa9wq2xtMvRm2Q4iz0ixaKJiwLVDsTzVLRPYbJ3ZAhZpQM_rSpfeA8-at-cipher.nrlssc.navy.mil} 3, 23 -- Subject: Position opening, electron microscopist 3, 23 -- Date: Wed, 17 Sep 2008 07:12:25 -0500 3, 23 -- MIME-Version: 1.0 3, 23 -- Content-Type: text/plain; 3, 23 -- charset="US-ASCII" 3, 23 -- In-Reply-To: {vymDT0kvPb1I2vmuWPNComqgOoQtMcp4Qr_gnFELw1eLqu1vWY6T5og44xn3aBQTJ9Ocq9rO9BAI4JApeS1qo3aUGhY2obh7MsKTiTltbzc-at-cipher.nrlssc.navy.mil} 3, 23 -- Content-class: urn:content-classes:message 3, 23 -- X-MS-Has-Attach: 3, 23 -- X-MS-TNEF-Correlator: 3, 23 -- Thread-Topic: Position opening, electron microscopist 3, 23 -- Thread-Index: AckYGpp6lGcWZET5TOWa7mMJ4lEp3wAo+Gaw 3, 23 -- References: {vymDT0kvPb1I2vmuWPNComqgOoQtMcp4Qr_gnFELw1eLqu1vWY6T5og44xn3aBQTJ9Ocq9rO9BAI4JApeS1qo3aUGhY2obh7MsKTiTltbzc-at-cipher.nrlssc.navy.mil} 3, 23 -- From: "Furukawa, Yoko" {yoko.furukawa-at-nrlssc.navy.mil} 3, 23 -- To: {Microscopy-at-Microscopy.Com} 3, 23 -- Cc: "Furukawa, Yoko" {yoko.furukawa-at-nrlssc.navy.mil} 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8HCD1E0007784 ==============================End of - Headers==============================
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Email: SuzKD-at-aol.com Name: Susan Dauksis
Organization: CBHS
Title-Subject: [Filtered] Hank Beebe
Question:
I'm looking for Hank Beebe who worked for Kevex. He lived near Pittsburgh.
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Email: jaq1-at-msstate.edu Name: Joseph Querin
Organization: Mississippi State University
Title-Subject: [Filtered] Electron Backscatterd Diffraction of Ti-6Al-4V
Question: I have been trying to perform some OIMs using EBSD on Ti 6-4. The problem that I am running into is that none of the diffraction patterns solve for the beta phase. I have done some optical microscopy and know that it has an alpha-beta lamellar structure with approximately 12-13% beta.
Why am I not getting any diffraction patterns solved for beta titanium.
First off, how well polished is your specimen? If not well polished, you will likely get strange patterns which will not index correctly. Or you will get no patterns.
Which library are you using?
gary g.
At 07:03 PM 9/17/2008, you wrote:
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Colleagues, we are looking for the "communication card / board" for Zeiss / LEO 1530 Scanning Electron Microscope (built in 1999). This circuit board seems to be not available any more as a spare part from Zeiss / Leo. If you have any spare part for sale or an electric circuit diagram it would be most welcome.
If you have one or more of these boards in non-functioning condition and could be interested in refurbishing and repairing them - please feel free to contact me for the details.
Valery Ray
=========================== www.partbeamsystech.com www.freudlabs.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
Sent: Thursday, September 18, 2008 4:31 AM To: vray-at-partbeamsystech.com
Colleagues, we are looking for the "communication card / board" for Zeiss / LEO 1530 Scanning Electron Microscope (built in 1999). This circuit board seems to be not available any more as a spare part from Zeiss / Leo. If you have any spare part for sale or an electric circuit diagram it would be most welcome.
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Email: Ian.Harper-at-med.monash.edu.au Name: Ian Harper
Organization: Monash University
Title-Subject: [Filtered] Position Available in Australia: Imaging Research Fellow, Optical Microscopy
Question: Ref No: A089386 Research Fellow Monash Micro Imaging Facility at AMREP Central Clinical School Faculty of Medicine, Nursing and Health Sciences
Monash University is seeking an enthusiastic and energetic person to be responsible for the management and running of the Monash Micro Imaging Facility at Alfred Medical Research and Education Precinct (AMREP). You will provide expert support to over 500 AMREP scientists from Baker IDI and Burnet Institutes as well as Monash University. The position is located at AMREP in Prahran where it supports over $2 million of state of the art microscopy and digital imaging equipment, located throughout the complex. Through MMI, scientists from these institutions have access to a range of instruments including three confocal microscopes, a deconvolution microscope, a TIRF microscope, many optical and fluorescence microscopes, and supporting image analysis workstations. It is anticipated that further live cell and intravital imaging instruments will be added in the future. The position will also facilitate access to the MMI Clayton instruments (over $6.5 million) when required. These instruments include confocal and multiphoton microscopes, automated fluorescence and live cell imaging systems, scanning and transmission electron microscopes, and high end image analysis workstations.
Some of the tasks and responsibilities include: ï Management of the AMREP Monash Micro Imaging Facility; ï Provision of specialised imaging support, specifically in the development of advanced microscopy, live cell imaging and related technologies; ï Coordination of on-site microscopy training courses; ï Supervision of major Honours or Postgraduate research projects;
You will need: ï A PhD (or equivalent) qualification in cell/molecular biology or equivalent biomedical stream; ï A high level of interpersonal communication skills (written and verbal) and the ability to work with academic research staff, students, technical staff and industry; ï Ability to strategically plan, organise and achieve work targets.
The position is ideal for a highly organised imaging expert wanting a stimulating job, working with a dynamic team, whilst contributing to a priority health area.
Appointment will be made at a level appropriate to the successful applicantís qualifications, experience and in accordance with classification standards for each level.
Remuneration package : AU$53,297 - $72,332 / AU$76,140 - $90,417 pa Level A/Level B (includes employer superannuation).
Duration: Three-year appointment
Enquiries: Dr Ian Harper, tel. +61-3-9905 5635 or email ian.harper-at-med.monash.edu.au
Applications: By email addressed to Mr Ben Norman at ben.norman-at-med.monash.edu.au by 17/10/2008
Applications must address the selection criteria, quote the reference number and include curriculum vitae and the names and contact details of three referees.
Monash respects the privacy of your personal information. www.privacy.monash.edu.au
An Equal Opportunity Employer EOWA Employer of Choice for Women
Position description and further details available at: Monash Website link. http://sssd.adm.monash.edu.au/employ/job.asp?refnumber=A089386
I recently got an internship where my primary responsibility is to get an Amray 1820 SEM up and running. All of the documentation for this piece of equipment are missing, so I was wondering if someone might have a copy of the setup and installation or operator's manuals available as a PDF, or could perhaps point me in the right direction of some internet resources that would help me out.
Thank you for your time, Justin
==============================Original Headers============================== 6, 26 -- From jfutia-at-uwyo.edu Sun Sep 21 12:28:06 2008 6, 26 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8LHS6wf018349 6, 26 -- for {Microscopy-at-Microscopy.Com} ; Sun, 21 Sep 2008 12:28:06 -0500 6, 26 -- Received: from UWMAIL.uwyo.edu (uwmail.uwyo.edu [172.26.4.76]) 6, 26 -- by willowsprings.uwyo.edu (8.13.8/8.13.8) with ESMTP id m8LHS5ON028064 6, 26 -- for {Microscopy-at-Microscopy.Com} ; Sun, 21 Sep 2008 11:28:05 -0600 (MDT) 6, 26 -- (envelope-from jfutia-at-uwyo.edu) 6, 26 -- Received: from TELEGRAPH4.uwyo.edu ([10.84.60.119]) by UWMAIL.uwyo.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 26 -- Sun, 21 Sep 2008 11:28:04 -0600 6, 26 -- Received: from 69.145.238.231 ([69.145.238.231]) by TELEGRAPH4.uwyo.edu ([10.84.60.126]) via Exchange Front-End Server uwmail.uwyo.edu ([172.26.4.76]) with Microsoft Exchange Server HTTP-DAV ; 6, 26 -- Sun, 21 Sep 2008 17:28:04 +0000 6, 26 -- User-Agent: Microsoft-Entourage/12.12.0.080729 6, 26 -- Date: Sun, 21 Sep 2008 11:28:03 -0600 6, 26 -- Subject: SEM - Need Amray Documentation and Sample Help 6, 26 -- From: Justin Futia {jfutia-at-uwyo.edu} 6, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 26 -- Message-ID: {C4FBE043.384%jfutia-at-uwyo.edu} 6, 26 -- Thread-Topic: SEM - Need Amray Documentation and Sample Help 6, 26 -- Thread-Index: AckcD2a16hsSnrPoQVmZ3uSyolNG8w== 6, 26 -- Mime-version: 1.0 6, 26 -- Content-type: text/plain; 6, 26 -- charset="ISO-8859-1" 6, 26 -- X-OriginalArrivalTime: 21 Sep 2008 17:28:04.0682 (UTC) FILETIME=[67B5BAA0:01C91C0F] 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8LHS6wf018349 ==============================End of - Headers==============================
The Microscopy Society of the Ohio River Valley (MSORV) will be having it's Fall Meeting on Thursday October 2, 2008, 2-6PM, at Proctor & Gamble's Winton Hill Business Center, Cincinnati, OH.
This is one you don't want to miss! We have four interesting talks scheduled, along with at least nine Corporate Sponsors exhibiting.
1. "SBF-SEM: Serial block face microscopy" - Joel Mancuso (Gatan)
2. "Microscopy 'Goings On' at Miami University"' - Richard E. Edelmann, Ph.D. (Director, Miami University EM Facility)
3. "Developing a high-temperature, pressurized shear stage for DIC microscopy studies of structured fluids" - Geoffrey Reynolds, Ph.D. (Procter & Gamble)
4. "Digital SLR's in the Microscopy Lab" - Matt L. Duley (Miami University EM Facility)
Directions to Proctor & Gamble's Winton Hill Business Center and the complete agenda are located on our website: www.msorv.org.
RSVP: By September 26, 2008 to my e-mail address located on the website.
Although there is no registration fee for this meeting, it is suggested that you Pre-register so that an attendance list can be compiled for the lobby receptionist to work against when giving visitor badges out.
Hope to see you there!
Pamela F. Lloyd MSORV Secretary
==============================Original Headers============================== 13, 30 -- From Pamela.Lloyd-at-WPAFB.AF.MIL Mon Sep 22 09:16:49 2008 13, 30 -- Received: from fohfwb004.oh.afmc.af.mil (fohfwb004.oh.afmc.af.mil [131.28.29.208]) 13, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8MEGnKx004722 13, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 22 Sep 2008 09:16:49 -0500 13, 30 -- Received: from FOHMLRL03.enterprise.afmc.ds.af.mil (fohmlrl03.enterprise.afmc.ds.af.mil [131.28.34.157]) 13, 30 -- by fohfwb004.oh.afmc.af.mil with ESMTP id m8MEHNot001183 13, 30 -- for {Microscopy-at-Microscopy.Com} ; Mon, 22 Sep 2008 14:17:23 GMT 13, 30 -- X-AuditID: 831c229d-000012ac00000180-f0-48d7a8d00d2e 13, 30 -- Received: from FOHMLBH02.Enterprise.afmc.ds.af.mil ([10.1.1.1]) by FOHMLRL03.enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.1830); 13, 30 -- Mon, 22 Sep 2008 10:16:48 -0400 13, 30 -- Received: from VFOHMLAO13.Enterprise.afmc.ds.af.mil ([131.28.34.133]) by FOHMLBH02.Enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.2942); 13, 30 -- Mon, 22 Sep 2008 10:16:48 -0400 13, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 30 -- Content-class: urn:content-classes:message 13, 30 -- MIME-Version: 1.0 13, 30 -- Content-Type: text/plain; 13, 30 -- charset="us-ascii" 13, 30 -- Subject: MSORV (Microscopy Society of the Ohio River Valley) Fall 2008 Meeting 13, 30 -- Date: Mon, 22 Sep 2008 10:16:47 -0400 13, 30 -- Message-ID: {29A7C5F65D54744CBFD97CD62A33DEE903C818AC-at-VFOHMLAO13.Enterprise.afmc.ds.af.mil} 13, 30 -- X-MS-Has-Attach: 13, 30 -- X-MS-TNEF-Correlator: 13, 30 -- Thread-Topic: MSORV (Microscopy Society of the Ohio River Valley) Fall 2008 Meeting 13, 30 -- Thread-Index: AckcvdkO0/2g0dTTTfKeOXfcfKMqGw== 13, 30 -- From: "Lloyd, Pamela F CTR USAF AFMC AFRL/RXPJ" {Pamela.Lloyd-at-WPAFB.AF.MIL} 13, 30 -- To: {Microscopy-at-Microscopy.Com} 13, 30 -- X-OriginalArrivalTime: 22 Sep 2008 14:16:48.0702 (UTC) FILETIME=[D9E7E5E0:01C91CBD] 13, 30 -- X-Brightmail-Tracker: AAAAAA== 13, 30 -- Content-Transfer-Encoding: 8bit 13, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8MEGnKx004722 ==============================End of - Headers==============================
It is time to spend some more money. I am intending to buy a Vibratome or equivalent vibrating knife microtome. I am familiar with the original Vibratome model and the Leica equivalent. Are there other brands to consider? Does anyone have positive or negative experience with them? (if you want it private, send your comments directly to me and I won't reveal the source). Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
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Email: jbs-at-temple.edu Name: Joel Sheffield
Organization: Temple University
Title-Subject: [Filtered] Sad News -- Dan H. Moore
Question: Colleagues,
I regret to inform you that Dan H. Moore, one of the early members of EMSA and of NYSEM (New York Society of Electron Microscopists) passed away on July 20 of this year, at the age of 98. Dan was trained in physics, and made significant contributions to electrophoresis and ultracentrifugation in addition to electron microscopy. His studies of the physical structure of the mouse Mammary Tumor Virus (J. Cell Biol., Jan 1959; 5: 85 - 92) were classics of a multidisciplinary approach to a biological problem. I joined his laboratory at Rockefeller in 1963, and the experience was fundamental in my career from then on.
There will be a memorial in Philadelphia this Friday. If any of you have memories to share, please send them to me.
We would rather give away surplus embedding chemicals then add them to the waste stream. These are free for the taking, you pay/arrange for shipping and we will box them up for you. They are described as follows:
5) 450 ml DDSA specially distilled 10) 25 ml DMP 30 1) 500ml (?) triethylenetramnine tech 60% 1) JB 4 embedding kit .... 800 ml Solution A, 30 ml Solution B, 12 gm catalyst
Alan Stone ASTON
==============================Original Headers============================== 6, 19 -- From as-at-astonmet.com Tue Sep 23 07:26:49 2008 6, 19 -- Received: from outbound3.mail.tds.net (outbound3.mail.tds.net [216.170.230.93]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8NCQncP005024 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 23 Sep 2008 07:26:49 -0500 6, 19 -- Received: from outaamta01.mail.tds.net (outaamta01.mail.tds.net [216.170.230.31]) 6, 19 -- by outbound3.mail.tds.net (8.13.6/8.13.4) with ESMTP id m8NCQmW6009055 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 23 Sep 2008 07:26:49 -0500 6, 19 -- Received: from mozart.astonmet.com ([69.11.219.4]) 6, 19 -- by outaamta01.mail.tds.net with ESMTP 6, 19 -- id {20080923122643.GHKS12265.outaamta01.mail.tds.net-at-mozart.astonmet.com} 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 23 Sep 2008 07:26:43 -0500 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 19 -- Date: Tue, 23 Sep 2008 07:26:41 -0500 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- From: Alan Stone {as-at-astonmet.com} 6, 19 -- Subject: Embedding Chemicals to Give Away 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- Message-Id: {20080923122643.GHKS12265.outaamta01.mail.tds.net-at-mozart.astonmet.com} ==============================End of - Headers==============================
I am not an imaging expert, hence this probably very simple question that I wanted some help with. I am trying to carry out some X-ray diffraction measurement at high temperature and also record some time lapse images using a simple digital camera (simple lens with no iris). The problem I have is that the heating strip goes from virtually dark to white hot and is it actually the very bright end that is the problem. I was wondering if there was a simple way of fitting a filter of some sort (infrared?) to compensate for this. I have a 'c' mount fitting on the camera.
Any help appreciated.
Jonathan
Professor J.C. Knowles BSc(hons), PhD, FIMMM, CEng, FRSC, CSci Professor of Biomaterials Science Head of Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD. UK. Tel +44 (0)207 915 1189 Fax +44 (0)207 915 1227 Mobile 07785 313615 Skype Me Editor, Journal of Biomaterials Applications Email J.Knowles-at-eastman.ucl.ac.uk Eastman website; http://www.eastman.ucl.ac.uk Personal website; http://www.homepages.ucl.ac.uk/~sfhvjck/ This email represents the views of the sender alone and must not be construed as representing the views of the Eastman Dental Institute. It may contain confidential information and may be protected by law as a legally privileged document and copyright work. Its content should not be disclosed and it should not be given or copied to anyone other than the person(s) named or referenced above. If you have received this email in error, please contact the sender.
==============================Original Headers============================== 8, 25 -- From ugez323-at-yahoo.co.uk Tue Sep 23 09:38:14 2008 8, 25 -- Received: from vscani-e.ucl.ac.uk (vscani-e.ucl.ac.uk [144.82.108.33]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8NEcBtF025034 8, 25 -- for {Microscopy-at-Microscopy.Com} ; Tue, 23 Sep 2008 09:38:14 -0500 8, 25 -- Received: from biomat-1.eastman.ucl.ac.uk ([144.82.51.63] helo=edibmjk) 8, 25 -- by vscani-e.ucl.ac.uk with esmtp (Exim 4.60) 8, 25 -- (envelope-from {ugez323-at-yahoo.co.uk} ) 8, 25 -- id 1Ki91k-0007RD-ES 8, 25 -- for Microscopy-at-Microscopy.Com; Tue, 23 Sep 2008 15:38:08 +0100 8, 25 -- From: "ugez323" {ugez323-at-yahoo.co.uk} 8, 25 -- To: {Microscopy-at-Microscopy.Com} 8, 25 -- Subject: Solution to a (I think!) simple imaging problem 8, 25 -- Date: Tue, 23 Sep 2008 15:38:09 +0100 8, 25 -- Message-ID: {00c001c91d8a$0056ca30$01045e90$-at-co.uk} 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset="US-ASCII" 8, 25 -- Content-Transfer-Encoding: 7bit 8, 25 -- X-Mailer: Microsoft Office Outlook 12.0 8, 25 -- Thread-Index: AckdieCqLWjvZfJ9Rg6PblwRz2UAYgAABMeA 8, 25 -- Content-Language: en-gb 8, 25 -- X-UCL-MailScanner-Information: Please contact the UCL Helpdesk, helpdesk-at-ucl.ac.uk for more information 8, 25 -- X-UCL-MailScanner: Found to be clean 8, 25 -- X-UCL-MailScanner-From: ugez323-at-yahoo.co.uk 8, 25 -- X-Spam-Status: No ==============================End of - Headers==============================
FEI Company is currently searching for two Senior Field Service Engineers with a background supporting ebeam technology.
One position will support our SEM and SDB customers in the Chicago, IL. The other position will support our TEM products (including Titan) at Purdue University in Indiana.
If you are interested feel free to email me directly and I will send you back a full job description.
The best email to use is
joe.williamson-at-fei.com. Or you can go directly to www.fei.com/careers.
Thanks for your interest.
Joe Williamson Sr. Technical Recruiter FEI Company 5350 NE Dawson Creek Drive Hillsboro, OR 97124 (Ph) 503.726.2639 | 800.334.1403 Joe.Williamson-at-fei.com
This message may contain confidential information. If you received it in error, please delete it and notify the sender. Thank you.
==============================Original Headers============================== 9, 27 -- From Resume-at-fei.com Tue Sep 23 16:56:31 2008 9, 27 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8NLuU2A014376 9, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 23 Sep 2008 16:56:30 -0500 9, 27 -- X-WSS-ID: 0K7O4Y9-01-6FL-01 9, 27 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 9, 27 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 2F6809695F7 9, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 23 Sep 2008 14:56:32 -0700 (PDT) 9, 27 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 9, 27 -- Tue, 23 Sep 2008 14:56:29 -0700 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="us-ascii" 9, 27 -- Subject: FW: Job Opportunity at FEI Company in IN or IL (SEM, TEM, SDB) 9, 27 -- Date: Tue, 23 Sep 2008 14:56:21 -0700 9, 27 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB8141B208C-at-hlexc03.w2k.feico.com} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: Job Opportunity at FEI Company in IN or IL (SEM, TEM, SDB) 9, 27 -- Thread-Index: AckdxwpL/354Vx3mSeqmFsjaM+GfJQAABqag 9, 27 -- From: "Resume" {Resume-at-fei.com} 9, 27 -- To: {Microscopy-at-Microscopy.Com} 9, 27 -- X-OriginalArrivalTime: 23 Sep 2008 21:56:29.0589 (UTC) FILETIME=[3BCF0450:01C91DC7] 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8NLuU2A014376 ==============================End of - Headers==============================
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Email: gmartin-at-oxy.edu Name: Gary Martin
Organization: Occidental College, Los Angeles
Title-Subject: [Filtered] Benchtop SEMs
Question: Hi- I seen the flyers on the new benchtop SEMs from Hitachi, Nikon-Jeol, and Evex, but I'd appreciate hearing pros and cons from microscopists that have used the units for basic imaging of biological samples. thanks
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Question: There has been a sudden rise for current reading on the ion gun for the FEG region for our Tecnai. It is fluctuating between 1-2 uA. I think the maximum allowable value is around 0.5 uA. During the time frame this problem occurred, equipment was idle for about a week. So, I have no idea what migth be the root cause for this issue. Any ideas, comments?
I am trying to put together a short introduction to SEM and EDS analysis and would like to know if any of you have a CAD type line drawing of a typical modern SEM column.
Thanks
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 6, 23 -- From rbeavers-at-mail.smu.edu Wed Sep 24 14:59:17 2008 6, 23 -- Received: from s31xua.systems.smu.edu (s31xua.systems.smu.edu [129.119.70.72]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8OJxGPN023524 6, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Sep 2008 14:59:16 -0500 6, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xua.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 23 -- Wed, 24 Sep 2008 14:59:10 -0500 6, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 23 -- Content-class: urn:content-classes:message 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- charset="iso-8859-1" 6, 23 -- Subject: teaching helps 6, 23 -- Date: Wed, 24 Sep 2008 14:58:34 -0500 6, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B3052C2171-at-s31xe7.systems.smu.edu} 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- Thread-Topic: teaching helps 6, 23 -- Thread-Index: Ackef+0F0Mc2gp9+SgiiOYN/+k3zqA== 6, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 6, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 6, 23 -- X-OriginalArrivalTime: 24 Sep 2008 19:59:11.0023 (UTC) FILETIME=[02E8D3F0:01C91E80] 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8OJxGPN023524 ==============================End of - Headers==============================
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Email: reganhll-at-gmail.com Name: johnson
Organization: educational institute
Title-Subject: [Filtered] EDX system
Question: I want to understand how does the EDX system actually work.
I use horiba supplied EDX system with an atmospheric window equipped with lithium drifted sillicon dtetctor. I understand the generation of x rays and detection of these x rays by detector in the liquid nitrogen cooled EDX detector. I use a software supplied with the instrument called EMAX
} From this point the point of generation of this signal how does the } data gets analysed and displayed, There are terms like fit index and } some times though the spectra shows some peaks but the relative } weight percentages are in negative values.............!!!
I will be happy if people who have a detailed understanding of this phenomena comment on the entire process of translation of the electronic signal to the data display by the software happens if there is any detailed document available i would like to go through it.
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Email: mgb-at-ansto.gov.au Name: Mark Blackford
Organization: ANSTO
Title-Subject: [Filtered] STEM-HAADF procedure
Question: Hi All,
does anyone have a procedure they would like to share for setting up lens configurations and calibrating collection angles for STEM-HAADF imaging with a JEOL 2010F (S)TEM?
Any advice or references would be appreciated. Cheers,
Dear listers, I am posting this on behalf of a colleague. Please do not reply to me, but to Paul Midgley whose address is below.
Many thanks
Richard Beanland
Post-Doctoral Research Associate
The Development of 3D Mesoscale Imaging and Analysis using a SEM-FIB Dual-Beam Microscope University of Cambridge, UK
A Post-Doctoral Research Associate position is available immediately to pursue a programme of research into the development of 3D mesoscale imaging and analysis using a SEM-FIB dual-beam electron microscope. The successful applicant will be responsible for many of the day-to-day activities of a new high resolution dual-beam instrument (FEI Helios) and engage in a variety of projects including developing 3D SEM imaging, 3D diffraction using EBSD, 3D microanalysis, advanced TEM sample preparation, device fabrication, low voltage STEM. Applicants must have expertise in scanning electron microscopy and focused ion beam milling and experience of a dual-beam instrument would be an advantage. Knowledge of one or more of the following would be desirable: TEM sample preparation, analysis of EBSD patterns and orientation imaging, quantitative X-ray microanalysis, image processing, nanoscale device fabrication, STEM. The position, funded by the EPSRC, is available immediately and for up to 2 years. The starting salary will be on the University post-doctoral scale, £25,888 - £33,780 pa, depending on experience.
Informal enquiries can be made to Prof Paul Midgley, e-mail: pam33-at-cam.ac.uk
Applications should be sent by post to Prof P.A. Midgley, Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge, CB2 3QZ, UK, and should include a full CV with the names and addresses of two referees. Closing date: 3rd October 2008.
==============================Original Headers============================== 10, 22 -- From rb258-at-hermes.cam.ac.uk Thu Sep 25 11:10:51 2008 10, 22 -- Received: from ppsw-0.csi.cam.ac.uk (ppsw-0.csi.cam.ac.uk [131.111.8.130]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8PGAp9j002642 10, 22 -- for {microscopy-at-microscopy.com} ; Thu, 25 Sep 2008 11:10:51 -0500 10, 22 -- X-Cam-AntiVirus: no malware found 10, 22 -- X-Cam-SpamDetails: not scanned 10, 22 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 10, 22 -- Received: from canigou.msm.cam.ac.uk ([131.111.102.43]:1284) 10, 22 -- by ppsw-0.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.150]:587) 10, 22 -- with esmtpsa (PLAIN:rb258) (TLSv1:DHE-RSA-AES256-SHA:256) 10, 22 -- id 1KitQY-00067u-34 (Exim 4.70) for microscopy-at-microscopy.com 10, 22 -- (return-path {rb258-at-hermes.cam.ac.uk} ); Thu, 25 Sep 2008 17:10:50 +0100 10, 22 -- Message-ID: {48DBB81F.9040102-at-integrityscientific.com} 10, 22 -- Date: Thu, 25 Sep 2008 17:11:11 +0100 10, 22 -- From: Richard Beanland {contact-at-integrityscientific.com} 10, 22 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) 10, 22 -- MIME-Version: 1.0 10, 22 -- To: microscopy-at-microscopy.com 10, 22 -- Subject: Position available 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 8bit 10, 22 -- Sender: "Dr R. Beanland" {rb258-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
Anyone familar with the Gillette-Siebert Confluorescence light microscope? "Confluorescence", not "confocal". Looks like a smallish, transmitted-fluorescence upright 'scope, with a large, integrated lamp housing and an obliquely set double wheel in the light path. Separation of the "double wheel" wheels is about 5 mm -- assuming it's not a single wheel with a deep groove in the rim. There's a filter wheel below the condensor. No manuals, and I'm not finding anything searching the web. Looks to be 50s vintage, may be early 60s. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Dear Johnson, I have found that the best teaching materials in EDX are from the Oxford web site. (http://www.x-raymicroanalysis.com/pages/main/main.htm). They have a very complete explanation of how the EDX hardware and software works. If you are getting nonsense from your EDX results, the first thing to determine is if your data is what the software is expecting. It sounds like a software problem, but the software can only work with the data it is given. Is your spectrum the right shape? Regards,
-----Original Message----- X-from: reganhll-at-gmail.com [mailto:reganhll-at-gmail.com] Sent: September 24, 2008 5:51 PM To: maryflet-at-interchange.ubc.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reganhll-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reganhll-at-gmail.com Name: johnson
Organization: educational institute
Title-Subject: [Filtered] EDX system
Question: I want to understand how does the EDX system actually work.
I use horiba supplied EDX system with an atmospheric window equipped with lithium drifted sillicon dtetctor. I understand the generation of x rays and detection of these x rays by detector in the liquid nitrogen cooled EDX detector. I use a software supplied with the instrument called EMAX
} From this point the point of generation of this signal how does the } data gets analysed and displayed, There are terms like fit index and } some times though the spectra shows some peaks but the relative } weight percentages are in negative values.............!!!
I will be happy if people who have a detailed understanding of this phenomena comment on the entire process of translation of the electronic signal to the data display by the software happens if there is any detailed document available i would like to go through it.
Next week a distinguished panel will be meeting to consider the current challenges and future directions in imagining local phenomena with scanning probes. The *Frontiers of Functional Imaging * workshop is sponsored by Basic Energy Science at the Department of Energy. The resulting report will be published and we hope be useful in informing strategic planning in this field.
On *_*Monday Sept 29, 3:15 - 4:30*_* the NanoProbe Network will host a 'live' forum to gather input from the community at large. The forum is an on-line discussion platform that is active now. During the 'live' time period the panelists will be interacting in real time.
We encourage you to post your questions, comments, views and perspectives, anytime before or after the 'live session' but especially to join us then. The site is at http://nanoprobenetwork.org/
You need to register (it takes 1 minute) in order to participate in the discussion, but you may view the discussion without registering.
We look forward to an invigorating discussion next Monday.
Panelists: Dawn Bonnell, Penn & Seamus Davis, Cornell Organizers
Dan Rugar, IBM
Chris Hammel, Ohio State Matthias Bode, Argonne National Lab
Kathryn Moler, Stanford University
Ward Plummer, U Tenn
Vidya Madhavan, Boston College
Dimitri Basov, UCSD
Sergei Kalinin, ORNL
Robert Westervelt, Harvard
Ray Ashoori, MIT
Miguel Salmeron, LBL Paul Weiss, Penn State
Norm Bartelt, Sandia Livermore
Ulrike Diebold, Tulane University
Udo Schwartz, Yale
Lucas Novotny, U of Rochester
-- Dawn Bonnell Trustee Chair Professor, Materials Science www.seas.upenn.edu/~bonnell Director, Nano/Bio Interface Center www.nanotech.upenn.edu 3231 Walnut St Philadelphia, PA 19104
215 898 6231
==============================Original Headers============================== 26, 24 -- From sergei2-at-ornl.gov Thu Sep 25 11:51:31 2008 26, 24 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 26, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8PGpVxj011503 26, 24 -- for {microscopy-at-microscopy.com} ; Thu, 25 Sep 2008 11:51:31 -0500 26, 24 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 26, 24 -- by emroute3.ornl.gov (PMDF V6.4 #31561) 26, 24 -- with ESMTP id {0K7R00KFGG5U4U-at-emroute3.ornl.gov} for 26, 24 -- microscopy-at-microscopy.com; Thu, 25 Sep 2008 12:51:30 -0400 (EDT) 26, 24 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 26, 24 -- (PMDF V6.4 #31561) id {0K7R00L01G5UB2-at-emroute3.ornl.gov} ; Thu, 26, 24 -- 25 Sep 2008 12:51:30 -0400 (EDT) 26, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 26, 24 -- by emroute3.ornl.gov (PMDF V6.4 #31561) 26, 24 -- with ESMTP id {0K7R00KBUG5U7U-at-emroute3.ornl.gov} ; Thu, 26, 24 -- 25 Sep 2008 12:51:30 -0400 (EDT) 26, 24 -- Date: Thu, 25 Sep 2008 12:51:30 -0400 26, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 26, 24 -- Subject: Frontiers of Functional Imaging - DOE BES 26, 24 -- To: microscopy-at-microscopy.com, "Dawn A. Bonnell" {bonnell-at-lrsm.upenn.edu} 26, 24 -- Message-id: {48DBC192.7090104-at-ornl.gov} 26, 24 -- MIME-version: 1.0 26, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 26, 24 -- Content-transfer-encoding: 7bit 26, 24 -- User-Agent: Thunderbird 2.0.0.16 (Windows/20080708) ==============================End of - Headers==============================
This site has a nice tutorial on EDS that could be useful for newcomers to the field. I am recommending this to others.
Thanks,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
maryflet-at-inter change.ubc.ca To gary.m.brown-at-exxonmobil.com 09/25/08 11:46 cc AM Subject [Microscopy] RE: viaWWW: I want to Please respond understand how does the EDX system to maryflet-at-inter change.ubc.ca
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Johnson, I have found that the best teaching materials in EDX are from the Oxford web site. (http://www.x-raymicroanalysis.com/pages/main/main.htm). They have a very complete explanation of how the EDX hardware and software works. If you are getting nonsense from your EDX results, the first thing to determine is if your data is what the software is expecting. It sounds like a software problem, but the software can only work with the data it is given. Is your spectrum the right shape? Regards,
-----Original Message----- X-from: reganhll-at-gmail.com [mailto:reganhll-at-gmail.com] Sent: September 24, 2008 5:51 PM To: maryflet-at-interchange.ubc.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reganhll-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reganhll-at-gmail.com Name: johnson
Organization: educational institute
Title-Subject: [Filtered] EDX system
Question: I want to understand how does the EDX system actually work.
I use horiba supplied EDX system with an atmospheric window equipped with lithium drifted sillicon dtetctor. I understand the generation of x rays and detection of these x rays by detector in the liquid nitrogen cooled EDX detector. I use a software supplied with the instrument called EMAX
} From this point the point of generation of this signal how does the } data gets analysed and displayed, There are terms like fit index and } some times though the spectra shows some peaks but the relative } weight percentages are in negative values.............!!!
I will be happy if people who have a detailed understanding of this phenomena comment on the entire process of translation of the electronic signal to the data display by the software happens if there is any detailed document available i would like to go through it.
} FOCUS ON MICROSCOPY 2009 - Krakow, Poland, 5 - 8 April 2009 } } 22nd International Conference on 3D Image Processing in Microscopy } 21st International Conference on Confocal Microscopy } } Dear Colleagues, } } After the successful FOM2008 conference held in Osaka, Awaji Island, Japan } this year, it is a pleasure to announce the next conference Focus on } Microscopy 2009. It will take place in Krakow, Poland, in the week before } Easter from Sunday April 5 to Wednesday April 8, 2009. } } As the next in a series of unique interdisciplinary meetings on advanced } multi-dimensional light microscopy and image processing, the conference } will be hosted by Jagliellonian University in Krakow at the Auditorium } Maximum building in the center of this historic and beautiful city. } } Please note that the conference outing and dinner will take place in the } afternoon and evening of the last day of the conference (i.e. Wednesday). } This in contrast to previous years, where this dinner was arranged earlier } in the conference. We are planning to visit the famous millennium old Salt } Mines near Krakow as part of the outing. } } Focus on Microscopy 2009 is the continuation of a yearly conference series } presenting the latest innovations and new trends in optical microscopy and } their application in biology, medicine and material sciences. } } Key subjects are: } } * the theory and practice of multi-dimensional optical microscopes, } * the high spatial resolution and sophisticated excitation (SHG, Raman, } THG, OCS) and fluorescence imaging modes (FRET,FLIM, FRAP, FCS) coming } available, } * new techniques for functional studies and manipulation of cells and } tissues, } * automated and high-throughput microscopy techniques, } * related image processing and analysis approaches. } } The conference series is, in addition, known for covering the rapid } development of advanced fluorescence labeling techniques for the confocal } and multi-photon 3D imaging of -live- biological specimens. Laser light } based activation and quenching techniques integrated with 3D microscopy } are becoming important tools in cell biology. } } Abstracts for contributions are invited and can already be submitted } through the website: http://www.FocusOnMicroscopy.org where further } information on the present and previous FOM conferences can be found. } } Important dates: } } Deadline for the submission of abstracts: January 19, 2009 } Acceptance of contributions, draft program: February 10, 2009 } Deadline for early registration: February 28, 2009 } } We welcome you to Krakow for the FOM2009 conference and exhibition. To } stay informed about the conference please leave your name and email at: } http://www.FocusOnMicroscopy.org/stayinformed } } On behalf of the organizing committee, } } - Jurek Dobrucki, Jagliellonian University, Krakow, Poland } - Fred Brakenhoff, Swammerdam Institute for Life Sciences, University of } Amsterdam, The Netherlands } -- } E-mail: info2009-at-FocusOnMicroscopy.org } Web: www.FocusOnMicroscopy.org } }
==============================Original Headers============================== 2, 24 -- From hubner-at-iod.krakow.pl Fri Sep 26 01:05:11 2008 2, 24 -- Received: from sowa.iod.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8Q659EM016569 2, 24 -- for {microscopy-at-microscopy.com} ; Fri, 26 Sep 2008 01:05:10 -0500 2, 24 -- Received: from iodkh007 (sroka.IOd.krakow.pl [149.156.29.200]) 2, 24 -- by sowa.iod.krakow.pl (8.13.6+Sun/8.13.6) with SMTP id m8Q5jfh8018642; 2, 24 -- Fri, 26 Sep 2008 07:45:41 +0200 (CEST) 2, 24 -- Message-ID: {8E4AE35617694D62BA746CD99ECE9116-at-iodkh007} 2, 24 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-iod.krakow.pl} 2, 24 -- To: "Microscopy list" {microscopy-at-microscopy.com} , 2, 24 -- "Materials" {MATERIALS-at-liverpool.ac.uk} , 2, 24 -- "Odlewnicza-pl" {odlew-pl-at-man.lodz.pl} 2, 24 -- Subject: Focus on Microscopy FOM2009, Krakow, Poland, 5 - 8 April 2009, 1st announcement 2, 24 -- Date: Fri, 26 Sep 2008 08:05:05 +0200 2, 24 -- MIME-Version: 1.0 2, 24 -- Content-Type: text/plain; 2, 24 -- format=flowed; 2, 24 -- charset="iso-8859-2"; 2, 24 -- reply-type=original 2, 24 -- Content-Transfer-Encoding: 7bit 2, 24 -- X-Priority: 3 2, 24 -- X-MSMail-Priority: Normal 2, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 2, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Looking for opinions: Water cooled vs Air cooled water chillers for EM´s. We are in the process of building a new microscopy facility and the question becomes air-cooled or water cooled chillers - which way to go. Now, I am NOT interested in running the scopes themselves off of a chilled building water loop, there will be dedicated water chillers for the scopes, it is cooling the refrigerant systems of the scope chillers I.e. water or air cooled condensers on the water chillers.
For years I worked with Air cooled chillers maintenance consisted of replacing pumps, pump couplings and pump motors occasionally. And cleaning the condenser coils. Never had a noticeable problem with air temperature effecting scope water loop.
For the last 14 years I´ve been working with water cooled condensers still replacing pumps & motors, but also adding significant repairs do to rotting out condensers which usually causes the compressors to go as well. But these have been chilled with city water not a building closed water loop (i.e. non-treated corrosive city water). Is this a significant issue with closed building chiller loops? Secondly, in general do folks using building water cooled condensers have issues with fluxuation or loss of the building water loop? (Yes, I realize these issue may depend on how well the building system is maintained but in general)
Air cooled would have to deal with noise and ventilation of the utility space the chillers sit in. Plus we have water chilled condensers presently and would have to replace the four chillers. Plus in order to deal with noise the air-cooled chillers may have to be placed further away and then have longer runs to the scopes. But this may really help with any noise issues.
Water cooled are quieter and would not need significant ventilation. We currently have water chilled condenser systems. BUT the building that will house the new facility does not have it own building chilled water loop so the cost of bringing the chilled water (for the condensers not the scopes) from the next door building (70 meters) does play into it.
So cost is New chillers, ventilation, and noise abatement, vs piping water from next door building. All of which may balance out. In which I´m back to the best solution in terms of the scopes and scope environment.
Any suggestions?
Thank you. Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 12, 23 -- From edelmare-at-muohio.edu Mon Sep 29 08:24:15 2008 12, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8TDOElc003348 12, 23 -- for {microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 08:24:15 -0500 12, 23 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 12, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m8TDOE3V029308 12, 23 -- for {microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 09:24:14 -0400 12, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 12, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m8TDOELK020486 12, 23 -- for {microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 09:24:14 -0400 12, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 23 -- To: microscopy-at-Microscopy.com 12, 23 -- Date: Mon, 29 Sep 2008 09:23:25 -0400 12, 23 -- MIME-Version: 1.0 12, 23 -- Subject: Water vs Air cooled water chillers for EM's 12, 23 -- Message-ID: {48E09E8D.20831.4C40EF-at-edelmare.muohio.edu} 12, 23 -- Priority: normal 12, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 23 -- Content-type: text/plain; charset=ISO-8859-1 12, 23 -- Content-description: Mail message body 12, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id m8TDOElc003348 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both parmiterd-at-ncifcrf.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: parmiterd-at-ncifcrf.gov Name: David Parmiter
I'm wondering if anyone has had any experience imaging Tantalum particles about 1-2nm in size. For whatever reason, I seem to be having trouble visualizing them. Are these generally less electron dense than gold particles, making them less visible to an EM (Ta does have a slightly lower atomic #) and should I therefore try either negative staining or some other course of action?
If your options include water cooled and air cooled chillers, then the heat load must not be too great. If it was, water cooled would be the only option. I'd suggest air cooled. Simple, effective, easy to maintain. Haskris chillers that are air cooled don't make a lot of noise. The Merlins also are quiet. A big issue is the reservoir volume and the amount of water turnover per hour--flow rate. Haskris is good since when you need parts, the come from IL rather than EU or other laborious route.
gary g.
At 06:26 AM 9/29/2008, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 22 -- From gary-at-gaugler.com Mon Sep 29 11:44:51 2008 9, 22 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m8TGiooH008029 9, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 11:44:51 -0500 9, 22 -- Message-Id: {200809291644.m8TGiooH008029-at-ns.microscopy.com} 9, 22 -- Received: (qmail 7603 invoked from network); 29 Sep 2008 09:43:45 -0700 9, 22 -- Received: by simscan 1.1.0 ppid: 7595, pid: 7596, t: 0.1694s 9, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 22 -- by smtp2 with SMTP; 29 Sep 2008 09:43:45 -0700 9, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 22 -- Date: Mon, 29 Sep 2008 09:44:47 -0700 9, 22 -- To: edelmare-at-muohio.edu 9, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 22 -- Subject: Re: [Microscopy] Water vs Air cooled water chillers for EM's 9, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 22 -- In-Reply-To: {200809291326.m8TDQeiw005850-at-ns.microscopy.com} 9, 22 -- References: {200809291326.m8TDQeiw005850-at-ns.microscopy.com} 9, 22 -- Mime-Version: 1.0 9, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8TGiooH008029 ==============================End of - Headers==============================
On Sep 29, 2008, at 7:10 AM, parmiterd-at-ncifcrf.gov wrote:
} I'm wondering if anyone has had any experience imaging Tantalum } particles about 1-2nm in size. For whatever reason, I seem to be } having trouble visualizing them. Are these generally less electron } dense than gold particles, making them less visible to an EM (Ta does } have a slightly lower atomic #) and should I therefore try either } negative staining or some other course of action?
Dear David, The Ta particles you're looking for are pretty small. Gold beads of that size often have to be enhanced in order to see them, but this depends on the context. Are you looking for isolated particles on a low-Z substrate, or are they used to label a specimen? I would not think that negative staining would make the particles more visible-- there is not as much difference between Z = 74 (Ta) and Z = 92 (U) as between Z = 74 and Z = 6. I do not know whether there is a chemical process that can be used to enhance the Ta, but if there is, I'd suggest that. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Mon Sep 29 12:02:43 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8TH2hR9022132 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 12:02:43 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 131C566E0906 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 10:02:43 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id F0C4966E0AE8 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 10:02:38 -0700 (PDT) 6, 22 -- Message-Id: {D76C5168-F352-4A6E-9883-A3A1E5CBB533-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200809291410.m8TEAQgE018795-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] viaWWW: Imaging Tantalum particles 6, 22 -- Date: Mon, 29 Sep 2008 10:02:38 -0700 6, 22 -- References: {200809291410.m8TEAQgE018795-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
My experience has been that building services have been quite good for water in terms of water temperature and pressure, and that corrosion in a closed system is minimal. However, they occasionally have emergency shutdowns due to leaks and shutdowns for preventive maintenance. Air conditioning, on the other hand, is just terrible in terms of reliability, but there is at least usually some air. I would worry about the air temperature range that air cooled chillers can accommodate. At worst, you could have a chiller room that was very uncomfortably hot.
John Mardinly, Numonyx -----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Monday, September 29, 2008 6:36 AM To: MARDINLY, A
Looking for opinions: Water cooled vs Air cooled water chillers for EM´s. We are in the process of building a new microscopy facility and the question becomes air-cooled or water cooled chillers - which way to go. Now, I am NOT interested in running the scopes themselves off of a chilled building water loop, there will be dedicated water chillers for the scopes, it is cooling the refrigerant systems of the scope chillers I.e. water or air cooled condensers on the water chillers.
For years I worked with Air cooled chillers maintenance consisted of replacing pumps, pump couplings and pump motors occasionally. And cleaning the condenser coils. Never had a noticeable problem with air temperature effecting scope water loop.
For the last 14 years I´ve been working with water cooled condensers still replacing pumps & motors, but also adding significant repairs do to rotting out condensers which usually causes the compressors to go as well. But these have been chilled with city water not a building closed water loop (i.e. non-treated corrosive city water). Is this a significant issue with closed building chiller loops? Secondly, in general do folks using building water cooled condensers have issues with fluxuation or loss of the building water loop? (Yes, I realize these issue may depend on how well the building system is maintained but in general)
Air cooled would have to deal with noise and ventilation of the utility space the chillers sit in. Plus we have water chilled condensers presently and would have to replace the four chillers. Plus in order to deal with noise the air-cooled chillers may have to be placed further away and then have longer runs to the scopes. But this may really help with any noise issues.
Water cooled are quieter and would not need significant ventilation. We currently have water chilled condenser systems. BUT the building that will house the new facility does not have it own building chilled water loop so the cost of bringing the chilled water (for the condensers not the scopes) from the next door building (70 meters) does play into it.
So cost is New chillers, ventilation, and noise abatement, vs piping water from next door building. All of which may balance out. In which I´m back to the best solution in terms of the scopes and scope environment.
Any suggestions?
Thank you. Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 12, 23 -- From edelmare-at-muohio.edu Mon Sep 29 08:24:15 2008 12, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8TDOElc003348 12, 23 -- for {microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 08:24:15 -0500 12, 23 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 12, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m8TDOE3V029308 12, 23 -- for {microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 09:24:14 -0400 12, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 12, 23 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m8TDOELK020486 12, 23 -- for {microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 09:24:14 -0400 12, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 23 -- To: microscopy-at-Microscopy.com 12, 23 -- Date: Mon, 29 Sep 2008 09:23:25 -0400 12, 23 -- MIME-Version: 1.0 12, 23 -- Subject: Water vs Air cooled water chillers for EM's 12, 23 -- Message-ID: {48E09E8D.20831.4C40EF-at-edelmare.muohio.edu} 12, 23 -- Priority: normal 12, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 23 -- Content-type: text/plain; charset=ISO-8859-1 12, 23 -- Content-description: Mail message body 12, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id m8TDOElc003348 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 29 -- From A.MARDINLY-at-numonyx.com Mon Sep 29 12:13:51 2008 20, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 20, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8THDpAP003258 20, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 12:13:51 -0500 20, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 20, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 8F3DF4141FF; 20, 29 -- Mon, 29 Sep 2008 12:39:25 -0400 (EDT) 20, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 20, 29 -- Mon, 29 Sep 2008 13:13:51 -0400 20, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 29 -- Content-class: urn:content-classes:message 20, 29 -- MIME-Version: 1.0 20, 29 -- Content-Type: text/plain; 20, 29 -- charset="iso-8859-1" 20, 29 -- Subject: RE: [Microscopy] Water vs Air cooled water chillers for EM's 20, 29 -- Date: Mon, 29 Sep 2008 13:13:48 -0400 20, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF992426F-at-EXDRESBENMX012.numonyx.local} 20, 29 -- In-Reply-To: {200809291335.m8TDZibJ015823-at-ns.microscopy.com} 20, 29 -- X-MS-Has-Attach: 20, 29 -- X-MS-TNEF-Correlator: 20, 29 -- Thread-Topic: [Microscopy] Water vs Air cooled water chillers for EM's 20, 29 -- Thread-Index: AckiOEkzCTzTY7ZtTge9pU4rEOPo/wAHcpbg 20, 29 -- References: {200809291335.m8TDZibJ015823-at-ns.microscopy.com} 20, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 20, 29 -- To: {edelmare-at-muohio.edu} 20, 29 -- Cc: {Microscopy-at-Microscopy.com} 20, 29 -- X-OriginalArrivalTime: 29 Sep 2008 17:13:51.0098 (UTC) FILETIME=[BE3D25A0:01C92256] 20, 29 -- Content-Transfer-Encoding: 8bit 20, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8THDpAP003258 ==============================End of - Headers==============================
We look at 10nm tantalum films regularly, and the contrast is extremely strong.
John Mardinly, Numonyx
-----Original Message----- X-from: parmiterd-at-ncifcrf.gov [mailto:parmiterd-at-ncifcrf.gov] Sent: Monday, September 29, 2008 7:18 AM To: MARDINLY, A
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both parmiterd-at-ncifcrf.gov as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: parmiterd-at-ncifcrf.gov Name: David Parmiter
I'm wondering if anyone has had any experience imaging Tantalum particles about 1-2nm in size. For whatever reason, I seem to be having trouble visualizing them. Are these generally less electron dense than gold particles, making them less visible to an EM (Ta does have a slightly lower atomic #) and should I therefore try either negative staining or some other course of action?
I'd like to add my observations. Air-cooled chillers work very well and Haskris chillers are great. We have one on both our SEM and our TEM. We designed a new facility a few years back and had purchased a new SEM that had an air-cooled Haskris chiller. While the performance of the chiller has been reliable and excellent, as most Haskris chillers typically perform, it is noisy and it does generate heat. We unfortunately allowed the in-house engineer who designed the room to persuade us to place the chiller in a closet within the SEM room. It seemed like a good idea at the time and we thought we would be able to close the closet door on it. Because of the heat the chiller generates, we cannot close the door, so we're dealing with the noise and heat. We'd like to move the chiller out but it will cost us several thousand dollars to relocate the chiller in a machine room down the hall.
Lesley
Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax)
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Monday, September 29, 2008 12:54 PM To: Lesley Bechtold
If your options include water cooled and air cooled chillers, then the heat load must not be too great. If it was, water cooled would be the only option. I'd suggest air cooled. Simple, effective, easy to maintain. Haskris chillers that are air cooled don't make a lot of noise. The Merlins also are quiet. A big issue is the reservoir volume and the amount of water turnover per hour--flow rate. Haskris is good since when you need parts, the come from IL rather than EU or other laborious route.
gary g.
At 06:26 AM 9/29/2008, you wrote:
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==============================Original Headers============================== 21, 35 -- From Lesley.Bechtold-at-jax.org Mon Sep 29 12:26:29 2008 21, 35 -- Received: from ms01.jax.org (ms01.jax.org [209.222.206.170]) 21, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8THQSUC030007 21, 35 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 12:26:29 -0500 21, 35 -- Received: from ms01.jax.org (localhost.localdomain [127.0.0.1]) 21, 35 -- by localhost (Email Security Appliance) with SMTP id 860BD135AF28; 21, 35 -- Mon, 29 Sep 2008 17:26:28 +0000 (GMT) 21, 35 -- Received: from jaxbhex02.jax.org (unknown [10.43.200.45]) 21, 35 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) 21, 35 -- (No client certificate requested) 21, 35 -- by ms01.jax.org (Email Security Appliance) with ESMTP id 09052135AF3E; 21, 35 -- Mon, 29 Sep 2008 17:26:23 +0000 (GMT) 21, 35 -- Received: from JAXBHMAIL01.jax.org ([10.43.200.39]) by jaxbhex02.jax.org 21, 35 -- ([10.43.200.45]) with mapi; Mon, 29 Sep 2008 13:26:22 -0400 21, 35 -- From: Lesley Bechtold {Lesley.Bechtold-at-jax.org} 21, 35 -- To: "gary-at-gaugler.com" {gary-at-gaugler.com} , 21, 35 -- "microscopy-at-microscopy.com" 21, 35 -- {microscopy-at-microscopy.com} , 21, 35 -- "edelmare-at-muohio.edu" {edelmare-at-muohio.edu} 21, 35 -- Date: Mon, 29 Sep 2008 13:26:21 -0400 21, 35 -- Subject: RE: [Microscopy] Re: Water vs Air cooled water chillers for EM's 21, 35 -- Thread-Topic: [Microscopy] Re: Water vs Air cooled water chillers for EM's 21, 35 -- Thread-Index: AckiU/eHebk9n7t0S7O/KJ/9ss/XhgAA190Q 21, 35 -- Message-ID: {3BDA51FFD1A83B4E90829F594A5C3717178F83CA79-at-JAXBHMAIL01.jax.org} 21, 35 -- References: {200809291653.m8TGrue7021429-at-ns.microscopy.com} 21, 35 -- In-Reply-To: {200809291653.m8TGrue7021429-at-ns.microscopy.com} 21, 35 -- Accept-Language: en-US 21, 35 -- Content-Language: en-US 21, 35 -- X-MS-Has-Attach: 21, 35 -- X-MS-TNEF-Correlator: 21, 35 -- acceptlanguage: en-US 21, 35 -- Content-Type: text/plain; charset="iso-8859-1" 21, 35 -- MIME-Version: 1.0 21, 35 -- Content-Transfer-Encoding: 8bit 21, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8THQSUC030007 ==============================End of - Headers==============================
On Sep 29, 2008, at 6:24 AM, edelmare-at-muohio.edu wrote:
} Looking for opinions: Water cooled vs Air cooled water chillers for } EM´s.
Dear Richard, For more than 20 years the HVEM at Albany has had air-cooled chillers with no problems. In addition to replacing the occasional motor or compressor, we checked the pH and concentration of an anti-corrosion treatment each month. Not only did this prevent the water from becoming too acid, it also gave us a chance to see the units, check the in-line filters for accumulated crud, etc. The Tecnai EMs in the cryoEM lab here were installed about six years ago with chillers that are cooled by a building chilled-water loop, and they also have performed well--in any case, the water cooling was not at fault in any chiller repair. On the one or two occasions when the building water failed, the chillers and microscopes shut down safely, and we had to put the scopes in a safe condition when we were notified of a planned chilled-water shutdown. All in all, I'd say that the two systems were both pretty reliable, and a one-time expense of bringing a chilled- water line into your facility would probably be cheaper than the alternative. Another consideration is that the Albany system had the chillers in a large open area, whereas the chillers here are in a small room, which makes air cooling less practical. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 7, 23 -- From tivol-at-caltech.edu Mon Sep 29 12:27:17 2008 7, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8THRGbQ031648 7, 23 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 12:27:17 -0500 7, 23 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 7, 23 -- by earth-doxen-postvirus (Postfix) with ESMTP id BCA8866E0AB7 7, 23 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 10:27:16 -0700 (PDT) 7, 23 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 7, 23 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 7, 23 -- by earth-doxen-ssl (Postfix) with ESMTP id 9EDE566E08EA 7, 23 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 10:27:15 -0700 (PDT) 7, 23 -- Message-Id: {E8EFB645-31F9-48CE-974E-D432B4C7013A-at-caltech.edu} 7, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- In-Reply-To: {200809291324.m8TDOR4O003477-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 7, 23 -- Mime-Version: 1.0 (Apple Message framework v929.2) 7, 23 -- Subject: Re: [Microscopy] Water vs Air cooled water chillers for EM's 7, 23 -- Date: Mon, 29 Sep 2008 10:27:15 -0700 7, 23 -- References: {200809291324.m8TDOR4O003477-at-ns.microscopy.com} 7, 23 -- X-Mailer: Apple Mail (2.929.2) 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8THRGbQ031648 ==============================End of - Headers==============================
I only have a little experience with this type of imaging. I assume that this is {particles} on a carbon film. Even for gold the mass of a 1 nm particle makes it MUCH harder to see than a 4nm particle (easy). 2nm is not even so bad even, but with 1nm gold, even on a very thin pure carbon film ( {3nm), the granularity of the carbon film seems to be on the same order as the particles and with the random variation in recorded density it leaves one wondering if you are really seeing the target particles. There must be some more sophisticated methods, but regarding simple visual or photographic visualization, and with a lower At#, I think it is not so easy. If it is easy, please direct us to a picture and a method!
Dale Callaham
A.MARDINLY-at-numonyx.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We look at 10nm tantalum films regularly, and the contrast is extremely } strong. } } John Mardinly, Numonyx } } } } -----Original Message----- } X-from: parmiterd-at-ncifcrf.gov [mailto:parmiterd-at-ncifcrf.gov] } Sent: Monday, September 29, 2008 7:18 AM } To: MARDINLY, A } Subject: [Microscopy] viaWWW: Imaging Tantalum particles } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ------------------------------------------------------------------------ } --- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both parmiterd-at-ncifcrf.gov as well as the MIcroscopy } Listserver } ------------------------------------------------------------------------ } --- } } Email: parmiterd-at-ncifcrf.gov } Name: David Parmiter } } Title-Subject: [Filtered] Imaging Tantalum particles } } Question: Hello all - } } I'm wondering if anyone has had any experience imaging Tantalum } particles about 1-2nm in size. For whatever reason, I seem to be } having trouble visualizing them. Are these generally less electron } dense than gold particles, making them less visible to an EM (Ta does } have a slightly lower atomic #) and should I therefore try either } negative staining or some other course of action? } } Thanks! } } Login Host: 129.43.43.117 } ------------------------------------------------------------------------ } --- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Mon Sep 29 09:10:16 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m8TEAFjr018660 } 7, 11 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 } 09:10:15 -0500 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240806c5069229c632-at-[206.69.208.22]} } 7, 11 -- Date: Mon, 29 Sep 2008 09:10:15 -0500 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: parmiterd-at-ncifcrf.gov (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Imaging Tantalum particles } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - } Headers============================== } } } } ==============================Original Headers============================== } 17, 29 -- From A.MARDINLY-at-numonyx.com Mon Sep 29 12:15:54 2008 } 17, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) } 17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8THFscV007258 } 17, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 29 Sep 2008 12:15:54 -0500 } 17, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) } 17, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 340171E8254; } 17, 29 -- Mon, 29 Sep 2008 11:16:04 -0400 (EDT) } 17, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); } 17, 29 -- Mon, 29 Sep 2008 13:15:53 -0400 } 17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 17, 29 -- Content-class: urn:content-classes:message } 17, 29 -- MIME-Version: 1.0 } 17, 29 -- Content-Type: text/plain; } 17, 29 -- charset="us-ascii" } 17, 29 -- Subject: RE: [Microscopy] viaWWW: Imaging Tantalum particles } 17, 29 -- Date: Mon, 29 Sep 2008 13:15:52 -0400 } 17, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9924270-at-EXDRESBENMX012.numonyx.local} } 17, 29 -- In-Reply-To: {200809291417.m8TEHiss030840-at-ns.microscopy.com} } 17, 29 -- X-MS-Has-Attach: } 17, 29 -- X-MS-TNEF-Correlator: } 17, 29 -- Thread-Topic: [Microscopy] viaWWW: Imaging Tantalum particles } 17, 29 -- Thread-Index: AckiPivZ9BbTQGbaTHqVMzjMAgyy4QAGLrWA } 17, 29 -- References: {200809291417.m8TEHiss030840-at-ns.microscopy.com} } 17, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} } 17, 29 -- To: {parmiterd-at-ncifcrf.gov} } 17, 29 -- Cc: {Microscopy-at-Microscopy.com} } 17, 29 -- X-OriginalArrivalTime: 29 Sep 2008 17:15:53.0492 (UTC) FILETIME=[0730FD40:01C92257] } 17, 29 -- Content-Transfer-Encoding: 8bit } 17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8THFscV007258 } ==============================End of - Headers==============================
On Sep 29, 2008, at 7:10 AM, parmiterd-at-ncifcrf.gov wrote:
} I'm wondering if anyone has had any experience imaging Tantalum } particles about 1-2nm in size. For whatever reason, I seem to be } having trouble visualizing them. Are these generally less electron } dense than gold particles, making them less visible to an EM (Ta does } have a slightly lower atomic #) and should I therefore try either } negative staining or some other course of action?
Dear David, I forgot to include the possibility of dark-field imaging in my previous message. If you have Ta on a low-Z substrate, dark field will show up the particles as bright spots, which are more easily distinguished than the small dark spots you'd observe in bright field. The down side is that the total number of counts in a dark field image is smaller that in bright field, but the Ta particles should not be too sensitive to a high dose, so that might not be a problem. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Mon Sep 29 12:34:37 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8THYbEs024648 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 12:34:37 -0500 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 387EE66E09A4 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 10:34:37 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 2288F66E0A36 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 10:34:36 -0700 (PDT) 6, 22 -- Message-Id: {FA55DE81-453A-42B0-956A-8FE520FDAFFD-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200809291410.m8TEAQgE018795-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] viaWWW: Imaging Tantalum particles 6, 22 -- Date: Mon, 29 Sep 2008 10:34:35 -0700 6, 22 -- References: {200809291410.m8TEAQgE018795-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
One of the most important things I would suggest is make sure your alignment of the machine is done correctly, (yes it sounds obvious, but often people will just give a rough alignment as they want to get on with their sample). Especially, if your NPs (nanoparticles) are on carbon film, the objective astigmatism, as it can make it very difficult to see particles if it is out. I did some analysis on gold NPs that were sub 1.5nm and whose contrast was very low. I found that they were best viewed with close to infocus conditions when the carbon background was at its minimum. I did not have any objective aperture in and was using the largest condenser aperture. You might get better contrast by selecting smaller aperture sizes. I am assuming it is a TEM we are talking about here.
Hope there is something in there of use.
Calum
-----Original Message----- X-from: parmiterd-at-ncifcrf.gov [mailto:parmiterd-at-ncifcrf.gov] Sent: 29 September 2008 15:18 To: calum.dickinson-at-ul.ie
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Email: parmiterd-at-ncifcrf.gov Name: David Parmiter
I'm wondering if anyone has had any experience imaging Tantalum particles about 1-2nm in size. For whatever reason, I seem to be having trouble visualizing them. Are these generally less electron dense than gold particles, making them less visible to an EM (Ta does have a slightly lower atomic #) and should I therefore try either negative staining or some other course of action?
Richard, My first question would be, "Have you looked at the water in the closed loop?" Some in-house cooled water loops are quite good and others, well, I wouldn't put that water in my car's cooling system. I've seen the exchangers get plugged and it took a while to diagnose the problem. If the water looks good and tests good chemically (Ph minerals, etc.), it may be a good alternative. In the winter, the added heat load from air cooled chiller-circulators can actually be a plus, although in the summer it can be an expensive drawback.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Monday, September 29, 2008 9:28 AM To: kenconverse-at-qualityimages.biz
Looking for opinions: Water cooled vs Air cooled water chillers for EM´s. We are in the process of building a new microscopy facility and the question becomes air-cooled or water cooled chillers - which way to go. Now, I am NOT interested in running the scopes themselves off of a chilled building water loop, there will be dedicated water chillers for the scopes, it is cooling the refrigerant systems of the scope chillers I.e. water or air cooled condensers on the water chillers.
For years I worked with Air cooled chillers maintenance consisted of replacing pumps, pump couplings and pump motors occasionally. And cleaning the condenser coils. Never had a noticeable problem with air temperature effecting scope water loop.
For the last 14 years I´ve been working with water cooled condensers still replacing pumps & motors, but also adding significant repairs do to rotting out condensers which usually causes the compressors to go as well. But these have been chilled with city water not a building closed water loop (i.e. non-treated corrosive city water). Is this a significant issue with closed building chiller loops? Secondly, in general do folks using building water cooled condensers have issues with fluxuation or loss of the building water loop? (Yes, I realize these issue may depend on how well the building system is maintained but in general)
Air cooled would have to deal with noise and ventilation of the utility space the chillers sit in. Plus we have water chilled condensers presently and would have to replace the four chillers. Plus in order to deal with noise the air-cooled chillers may have to be placed further away and then have longer runs to the scopes. But this may really help with any noise issues.
Water cooled are quieter and would not need significant ventilation. We currently have water chilled condenser systems. BUT the building that will house the new facility does not have it own building chilled water loop so the cost of bringing the chilled water (for the condensers not the scopes) from the next door building (70 meters) does play into it.
So cost is New chillers, ventilation, and noise abatement, vs piping water from next door building. All of which may balance out. In which I´m back to the best solution in terms of the scopes and scope environment.
Any suggestions?
Thank you. Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
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==============================Original Headers============================== 24, 26 -- From kenconverse-at-qualityimages.biz Mon Sep 29 13:03:18 2008 24, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 24, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8TI3HGX032481 24, 26 -- for {microscopy-at-microscopy.com} ; Mon, 29 Sep 2008 13:03:17 -0500 24, 26 -- Received: from Ken ([72.227.100.25]) by cdptpa-omta01.mail.rr.com 24, 26 -- with ESMTP 24, 26 -- id {20080929180317.ICOW19942.cdptpa-omta01.mail.rr.com-at-Ken} ; 24, 26 -- Mon, 29 Sep 2008 18:03:17 +0000 24, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 24, 26 -- To: {edelmare-at-muohio.edu} , "MSA Listserver" {microscopy-at-microscopy.com} , 24, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 24, 26 -- Subject: RE: [Microscopy] Water vs Air cooled water chillers for EM's 24, 26 -- Date: Mon, 29 Sep 2008 14:03:11 -0400 24, 26 -- Message-ID: {62BEB7AF249B4553A8FF437589C14AF1-at-Ken} 24, 26 -- MIME-Version: 1.0 24, 26 -- Content-Type: text/plain; 24, 26 -- charset="iso-8859-1" 24, 26 -- X-Priority: 3 (Normal) 24, 26 -- X-MSMail-Priority: Normal 24, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 24, 26 -- Importance: Normal 24, 26 -- Thread-Index: AckiN0MJTr80TGhtTR65+xXPtToFsgAJQ/kg 24, 26 -- In-Reply-To: {200809291328.m8TDSJV5007481-at-ns.microscopy.com} 24, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 24, 26 -- Content-Transfer-Encoding: 8bit 24, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m8TI3HGX032481 ==============================End of - Headers==============================
Haskris used to make water-cooled heat exchanger for use with the building water. It has no refrigerator - but only a valve regulating cold water flow (building water) through the heat exchanger coil submerged in the closed loop water reservoir. And of course a pump for closed loop water. Could be most reliable and least expensive solution. Plus most quiet and most compact.
Of course building water has to be colder than the closed loop water for this to work.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {edelmare-at-muohio.edu} To: {vitalylazar-at-att.net} Sent: Monday, September 29, 2008 9:26 AM
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: R & D
Title-Subject: [Filtered] TEM sample prep
Question: Dear All
Which is the best Ion Milling System to prepare the ceramic specimen for TEM analysis
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Question: I would appreciate recommendations from the group for photo quality printers. My facility prints approx. 1,000 clinical TEM photos per month. The final print needs to be an 8 X 10 glossy. We currently use a Codonics printer but really do not want to spend the $20,000 to replace it.
You should have a look on the JEOL Ion Slicer system. We have bought it this year and results are really impressive, moreover samples are easy to prepare compare to typical methods. We usually work on the following materials C/SiC/TiC/ZrC/ZrO/SiO.. However its cost is higher than the one of a classical ion milling system
Patrick Weisbecker
----- Message d'origine ---- De : "rashmi_mehata-at-yahoo.com" {rashmi_mehata-at-yahoo.com} À : weis183-at-yahoo.fr Envoyé le : Mardi, 30 Septembre 2008, 0h44mn 22s Objet : [Microscopy] viaWWW: TEM sample prep
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Email: rashmi_mehata-at-yahoo.com Name: Rashmi
Organization: R & D
Title-Subject: [Filtered] TEM sample prep
Question: Dear All
Which is the best Ion Milling System to prepare the ceramic specimen for TEM analysis
I've observed some Pd nanoparticles of 1-2nm and had also great difficulties to see them. Particularly because they were amorphous or poorly cristallised. The carbon film must be very thin (holey carbon with another thinner film in the holes). The imaging condition the best suited to see them was dark field imaging. Bright field with strong underfocus can also allow to see them but you can obtain only few information from this kind of imaging.
Patrick
----- Message d'origine ---- De : "calum.dickinson-at-ul.ie" {calum.dickinson-at-ul.ie} À : weis183-at-yahoo.fr Envoyé le : Lundi, 29 Septembre 2008, 19h41mn 15s Objet : [Microscopy] RE: viaWWW: Imaging Tantalum particles
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Dear David,
One of the most important things I would suggest is make sure your alignment of the machine is done correctly, (yes it sounds obvious, but often people will just give a rough alignment as they want to get on with their sample). Especially, if your NPs (nanoparticles) are on carbon film, the objective astigmatism, as it can make it very difficult to see particles if it is out. I did some analysis on gold NPs that were sub 1.5nm and whose contrast was very low. I found that they were best viewed with close to infocus conditions when the carbon background was at its minimum. I did not have any objective aperture in and was using the largest condenser aperture. You might get better contrast by selecting smaller aperture sizes. I am assuming it is a TEM we are talking about here.
Hope there is something in there of use.
Calum
-----Original Message----- X-from: parmiterd-at-ncifcrf.gov [mailto:parmiterd-at-ncifcrf.gov] Sent: 29 September 2008 15:18 To: calum.dickinson-at-ul.ie
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Email: parmiterd-at-ncifcrf.gov Name: David Parmiter
I'm wondering if anyone has had any experience imaging Tantalum particles about 1-2nm in size. For whatever reason, I seem to be having trouble visualizing them. Are these generally less electron dense than gold particles, making them less visible to an EM (Ta does have a slightly lower atomic #) and should I therefore try either negative staining or some other course of action?
find a Xerox dealer near you and bring your files for a test first. Prints will be semi-gloss, at a cost 5 cents and up depending on the quality of paper, and printer model.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {chesnutl-at-uthscsa.edu} To: {vitalylazar-at-att.net} Sent: Monday, September 29, 2008 6:41 PM
Here's some stuff for those collectors and historians of microscopy. I have the following company bulletins that are free to a new home (otherwise they go into the recycling bin...).
Hitachi: Hitachi News--17 items Technical note on High Resolution TEM Technical Data bulletin
JEOL: JEOL News--14 items A Guide to Scanning Microscope Observation Application Photos II
Zeiss: Zeiss Information--2 items
Phillips: Norelco Reporter--7 items Electron Optics Bulletin--9 items
How can one find out the names of institutions that have won R&D contracts and grants funded by the European Union? Is there a central website that gives such public information?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 3, 24 -- From donc-at-asmicro.com Wed Oct 1 16:27:01 2008 3, 24 -- Received: from smtp124.sbc.mail.re3.yahoo.com (smtp124.sbc.mail.re3.yahoo.com [66.196.96.97]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m91LR1fE027511 3, 24 -- for {microscopy-at-microscopy.com} ; Wed, 1 Oct 2008 16:27:01 -0500 3, 24 -- Received: (qmail 25246 invoked from network); 1 Oct 2008 21:27:01 -0000 3, 24 -- Received: from unknown (HELO asm15) (donc-at-76.240.197.108 with login) 3, 24 -- by smtp124.sbc.mail.re3.yahoo.com with SMTP; 1 Oct 2008 21:27:00 -0000 3, 24 -- X-YMail-OSG: t_eZozAVM1mHdMU_0PrjMIgA4EWocVQ6FnYjdKhYpeKN9_T_rx5Z7xSYENvNUVzBb8who4LhF21rqZGzkRqn1wGvanfdIh6W2VLVNPrMKa0k2mxILUcawyl7H977RzcjCN3M7heQjO360didl5uxcHprHIOTkd78eXKdv28YaIqCz7MTaw-- 3, 24 -- X-Yahoo-Newman-Property: ymail-3 3, 24 -- Message-ID: {B077A560ABD14D6C86748C34157E22F6-at-asm15} 3, 24 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 3, 24 -- To: "Microscopy List" {microscopy-at-microscopy.com} 3, 24 -- Subject: EU grants and contracts 3, 24 -- Date: Wed, 1 Oct 2008 17:26:18 -0400 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- format=flowed; 3, 24 -- charset="iso-8859-1"; 3, 24 -- reply-type=original 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Just wanted to inform those of you who know me that I have left the Albert Einstein College Of Medicine. My new contact information is in the sig below.
If anybody with light microscopy and computer image analysis experience is seeking a position in a shared resource, you may want to consider looking at the job posting at The Analytical Imaging Facility at AECOM http://www.aecom.yu.edu/aif/ This is a very well equipped and well regarded facility that could benefit from knowledgeable and strong leadership.
Thank you everybody for many years of great information and invaluable advice. I'm signing of the microscopy listserv for now, but hope that if I need you, you'll continue to be here going strong!
Sincerely-
Michael
-- Michael Cammer http://coxcammer.com/ michael-at-coxcammer.com or mcammer-at-gmail.com H 914-632-3044 Cell 914-309-3270
********* The address cammer-at-aecom.yu.edu was shut down 5 Sept. 2008. Any email sent there may not be delivered. *********
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Associate Development Engineer position in the College of Engineering Dean's Office at the University of California, Davis campus. Vacancy Listing #10979
Primary responsibilities are to operate and maintain a scanning electron microscope system equipped with writing capability in nano range in support of micro and nano fabrication research. Includes routine maintenance, train lab members in Electron Beam Lithography (EBL); work closely with students, faculty and industrial users. Also includes process development, equipment trouble shooting and maintenance of other micro and nano equipment such as e-beam evaporators, Nano-Imprint- Lithography and associated imaging equipment.
Salary Range $4,422 - $7,517 monthly
Please see the following links for a more detailed job description and vacancy announcement http://jobs.hr.ucdavis.edu/jm/ViewVacancy?id=10979 http://jobs.hr.ucdavis.edu/jm/PositionDescriptionView?id=10979
To apply for this position online and/or to download the application forms, please visit the UC Davis HR web site at: http://www.hr.ucdavis.edu/Emp/Application_Process/Application_Process
Fred A. Hayes Central Facilities Manager Dept of Chemical Engineering and Material Sciences 3118 Bainer Hall Univ of CA Davis Davis CA 95616 530-752-0284 office 530-754-6350 fax fahayes-at-ucdavis.edu http://www.matscicf.ucdavis.edu/
==============================Original Headers============================== 10, 19 -- From fahayes-at-ucdavis.edu Wed Oct 1 19:02:34 2008 10, 19 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9202YnK028510 10, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Oct 2008 19:02:34 -0500 10, 19 -- Received: from [169.237.230.156] ([169.237.230.156]) 10, 19 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id m9202XuG001584 10, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 1 Oct 2008 17:02:33 -0700 (PDT) 10, 19 -- Message-ID: {48E40F99.6020105-at-ucdavis.edu} 10, 19 -- Date: Wed, 01 Oct 2008 17:02:33 -0700 10, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 10, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Microscopy-at-Microscopy.Com 10, 19 -- Subject: SEM EBL position at UC Davis 10, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit 10, 19 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av7 10, 19 -- X-Virus-Status: Clean 10, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 ==============================End of - Headers==============================
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Email: clethrionomys-at-msn.com Name: sam
Title-Subject: [Filtered] adapting digital camera to zeiss photomicroscope
Question: Has anyone tried to adapt a digital camera to the 35mm film insert for the Zeiss Photomicroscope? I imagine one would have to destroy the 35mm apparatus and permanently fix the innards of a small digital camera into the shell so that the focal plane for the digital camera is exactly where the film would normally be. Does anyone know of a company or artisan who might undertake such a thing?
As you can guess, I am pretty attached to my photomicroscope but want the convenience of digital.
You can try http://cordis.europa.eu/home_en.html which is the portal to the information service of the European Union. The link to the database of EU funded projects since 1990 is http://cordis.europa.eu/search/index.cfm?fuseaction=proj.advSearch Then you may have to search per framework program (FP); currently running contracts would have been awarded under FP7 or FP6.
Kind regards Siegfried Jaecques K.U.Leuven - BIOMAT Research Cluster
Quoting donc-at-asmicro.com:
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I have a student that has taken a large number of SEM images at different magnifications and now wants to rescale all to a new uniform scale for print presentation.
All images were saved as 1024X768 pixel tiffs and do include a scale bar.
Images should have been taken at constant magnification but since that was not done, I am looking for ideas how to rescale these.
Thanks
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 23 -- From rbeavers-at-mail.smu.edu Fri Oct 3 13:25:41 2008 8, 23 -- Received: from s31xua.systems.smu.edu (s31xua.systems.smu.edu [129.119.70.72]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m93IPcIU015123 8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2008 13:25:40 -0500 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xua.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Fri, 3 Oct 2008 13:25:35 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="iso-8859-1" 8, 23 -- Subject: SEM Image scale 8, 23 -- Date: Fri, 3 Oct 2008 13:25:17 -0500 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B3053339BB-at-s31xe7.systems.smu.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: SEM Image scale 8, 23 -- Thread-Index: AcklhWLv5vUNsJ2vTfCcmrRf0MGnSA== 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 03 Oct 2008 18:25:36.0164 (UTC) FILETIME=[6DE91640:01C92585] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m93IPcIU015123 ==============================End of - Headers==============================
If the mmicron markers have the same values, then it is relatively easy thing to do. Just scale the second image by the ratio of the two lengths of the markers and you have it. However, if the markers have the different values, e.g. 1 µm and 5 µm, then you have to scale by the ratios of the Length/µm of one image to the length/µm of the other image. In Photoshop, you simply go into Image-Image Size and then if the new image will be smaller, you should use resample option. If it will be larger you should take resample off. Then go to width and change to percent. Use your ratio value and multiply it by 100 to get percent and change it making sure that the link is there for the height so that the aspect ratio stays constant. In one case your file size will be smaller and in the other case, your resolution of the image will get smaller.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Friday, October 03, 2008 11:33 AM To: Walck-at-SouthBayTech.com
Group,
I have a student that has taken a large number of SEM images at different magnifications and now wants to rescale all to a new uniform scale for print presentation.
All images were saved as 1024X768 pixel tiffs and do include a scale bar.
Images should have been taken at constant magnification but since that was not done, I am looking for ideas how to rescale these.
Thanks
Roy Beavers Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 8, 23 -- From rbeavers-at-mail.smu.edu Fri Oct 3 13:25:41 2008 8, 23 -- Received: from s31xua.systems.smu.edu (s31xua.systems.smu.edu [129.119.70.72]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m93IPcIU015123 8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2008 13:25:40 -0500 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xua.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 23 -- Fri, 3 Oct 2008 13:25:35 -0500 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="iso-8859-1" 8, 23 -- Subject: SEM Image scale 8, 23 -- Date: Fri, 3 Oct 2008 13:25:17 -0500 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B3053339BB-at-s31xe7.systems.smu.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: SEM Image scale 8, 23 -- Thread-Index: AcklhWLv5vUNsJ2vTfCcmrRf0MGnSA== 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 03 Oct 2008 18:25:36.0164 (UTC) FILETIME=[6DE91640:01C92585] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m93IPcIU015123 ==============================End of - Headers==============================
Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.138 / Virus Database: 270.6.3/1614 - Release Date: 8/15/2008 5:29 PM
==============================Original Headers============================== 19, 23 -- From walck-at-southbaytech.com Fri Oct 3 15:27:21 2008 19, 23 -- Received: from nlpi025.prodigy.net (nlpi025.sbcis.sbc.com [207.115.36.54]) 19, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m93KRK2G032578 19, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2008 15:27:20 -0500 19, 23 -- Received: from dynamicbl8uno3 (adsl-99-154-21-201.dsl.irvnca.sbcglobal.net [99.154.21.201]) 19, 23 -- (authenticated bits=0) 19, 23 -- by nlpi025.prodigy.net (8.13.8 smtpauth/dk/8.13.8) with ESMTP id m93KRIxV020787; 19, 23 -- Fri, 3 Oct 2008 15:27:19 -0500 19, 23 -- From: "Scott Walck" {walck-at-southbaytech.com} 19, 23 -- To: {Microscopy-at-microscopy.com} 19, 23 -- Cc: {rbeavers-at-mail.smu.edu} 19, 23 -- Subject: RE: [Microscopy] SEM Image scale 19, 23 -- Date: Fri, 3 Oct 2008 13:27:40 -0700 19, 23 -- Message-ID: {BCDF9B96C94C42E18BB4CED76C4F8012-at-dynamicbl8uno3} 19, 23 -- MIME-Version: 1.0 19, 23 -- Content-Type: text/plain; 19, 23 -- charset="iso-8859-1" 19, 23 -- X-Mailer: Microsoft Office Outlook 11 19, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 19, 23 -- Thread-Index: AcklhmxTKYOs0678Q8aH1cnrbEOoqQADqNLA 19, 23 -- In-Reply-To: {200810031832.m93IWXo8020325-at-ns.microscopy.com} 19, 23 -- Content-Transfer-Encoding: 8bit 19, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m93KRK2G032578 ==============================End of - Headers==============================
Do you have Photoshop? If so you can use the Layers and Opacity control, and maybe a little ImageJ (although I hear that the new version of Photoshop has some Scientific/Technical features that may somewhat do the things I normally do in ImageJ.
If you start with one image and paste another onto it it goes on as a new Layer, pixel for pixel. Be careful about resizing or altering the aspect ratio. If you set the opacity so that you can "see through", you can then (constraining aspect ratio..) resize one so the scale bars are the same - assuming the mags are such that the scalebar length is the same value on both. If the scale bars are different, I would use ImageJ; draw a line on the scalebar of one image and then use the Analyze-SetScale to tell it that "that many" pixels is 1um (for instance). Now that image is "calibrated" and when you draw a line on the image you can make a 0.5um or 2um scalebar so it will represent the same length as the "base image" you are trying to scale things to match; then do the overlay/opacity thing and resize to a uniform magnification. In the end the "other" layer can be deleted.
Hope this helps.
Dale
Dale Callaham Umass-at-Amherst
rbeavers-at-mail.smu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Group, } } I have a student that has taken a large number of SEM images at different magnifications and now wants to rescale all to a new uniform scale for print presentation. } } All images were saved as 1024X768 pixel tiffs and do include a scale bar. } } Images should have been taken at constant magnification but since that was not done, I am looking for ideas how to rescale these. } } Thanks } } Roy Beavers } Southern Methodist University } Department of Earth Sciences } P.O. Box 750395 } Dallas, TX 75275 } Voice: 214-768-2756 } Fax: 214-768-2701 } Email: rbeavers-at-smu.edu } } } } ==============================Original Headers============================== } 8, 23 -- From rbeavers-at-mail.smu.edu Fri Oct 3 13:25:41 2008 } 8, 23 -- Received: from s31xua.systems.smu.edu (s31xua.systems.smu.edu [129.119.70.72]) } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m93IPcIU015123 } 8, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2008 13:25:40 -0500 } 8, 23 -- Received: from s31xe7.systems.smu.edu ([129.119.70.140]) by s31xua.systems.smu.edu with Microsoft SMTPSVC(6.0.3790.3959); } 8, 23 -- Fri, 3 Oct 2008 13:25:35 -0500 } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 23 -- Content-class: urn:content-classes:message } 8, 23 -- MIME-Version: 1.0 } 8, 23 -- Content-Type: text/plain; } 8, 23 -- charset="iso-8859-1" } 8, 23 -- Subject: SEM Image scale } 8, 23 -- Date: Fri, 3 Oct 2008 13:25:17 -0500 } 8, 23 -- Message-ID: {F3733115F33FD244B4747E288321A2B3053339BB-at-s31xe7.systems.smu.edu} } 8, 23 -- X-MS-Has-Attach: } 8, 23 -- X-MS-TNEF-Correlator: } 8, 23 -- Thread-Topic: SEM Image scale } 8, 23 -- Thread-Index: AcklhWLv5vUNsJ2vTfCcmrRf0MGnSA== } 8, 23 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} } 8, 23 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} } 8, 23 -- X-OriginalArrivalTime: 03 Oct 2008 18:25:36.0164 (UTC) FILETIME=[6DE91640:01C92585] } 8, 23 -- Content-Transfer-Encoding: 8bit } 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m93IPcIU015123 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 22 -- From dac-at-research.umass.edu Fri Oct 3 15:43:13 2008 9, 22 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m93KhDJW013904 9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2008 15:43:13 -0500 9, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 9, 22 -- (authenticated bits=0) 9, 22 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m93KhCh6013508 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 9, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Oct 2008 16:43:13 -0400 9, 22 -- Message-ID: {48E69214.8030407-at-research.umass.edu} 9, 22 -- Date: Fri, 03 Oct 2008 16:43:48 -0500 9, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 9, 22 -- Reply-To: dac-at-research.umass.edu 9, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.16) Gecko/20080702 SeaMonkey/1.1.11 9, 22 -- MIME-Version: 1.0 9, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 22 -- Subject: Re: [Microscopy] SEM Image scale 9, 22 -- References: {200810031835.m93IZkHb023583-at-ns.microscopy.com} 9, 22 -- In-Reply-To: {200810031835.m93IZkHb023583-at-ns.microscopy.com} 9, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
We are looking for a 3D reconstruction software from tilted SEM images. Our goal is to use the SEM to complete the work which can be done with AFM, when the rugosity becomes to great.
I have soon some informations about Alicona's Mex tool (which is very expensive), and SamX 3D_TOPx.
I made a search in the Listserver archives and didn't find much, and on the web, I found plenty of serial section reconstruction softwares, but none (appart the two mentionned) working with SEM tilted images.
So I have two questions :
-first I would apreciate some feedback from user of these two tools, with the hanging question : is such a tool his price value ? The math behind is that used since 100 years for aerial stereo photos paires and stereogrammetry... There is "only" (of coarse a piece of work) a intelligent graphic interface to build !
-do someone know other softwares, preferentally free (!), open (!!) and OS independent (linux & winXP) (!!!).
A thank in advance
Jacques
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dingbiocmu-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] LM: wanted simple method for preparing chromosome spreads and karyotype analysis
Question: Dear listers, Kindly share me a simple method for preparing chromosome spreads of bone marrow ( mouse) and white blood cells ( human). We have in our department a bench centrifuge and a few reagents. Your help counts. Thanks
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Title-Subject: [Filtered] Looking For A Water Chiller
Question: We are looking for a used water chiller for a SEM that can run 5 liters a minute at 68F.Ý If there is any used chillers that are in working condition plese e-mail at brad.sprouse-at-psaengineering.com
We have a client who needs to use grids with a support film that will withstand annealing temperatures of 500 C or higher. Does anyone have any ideas what grid material might withstand this and whether or not a carbon support film will survive?
Thanks mucho, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 23 -- From TindallR-at-missouri.edu Mon Oct 6 10:52:00 2008 7, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96Fq0Fh030522 7, 23 -- for {microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 10:52:00 -0500 7, 23 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 23 -- Mon, 6 Oct 2008 10:51:56 -0500 7, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 23 -- Content-class: urn:content-classes:message 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: text/plain; 7, 23 -- charset="US-ASCII" 7, 23 -- Subject: TEM: Annealing grids with support film 7, 23 -- Date: Mon, 6 Oct 2008 10:51:55 -0500 7, 23 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7A17-at-UM-XMAIL08.um.umsystem.edu} 7, 23 -- X-MS-Has-Attach: 7, 23 -- X-MS-TNEF-Correlator: 7, 23 -- Thread-Topic: TEM: Annealing grids with support film 7, 23 -- thread-index: Ackny3S1/RNXYJ0sRzujl0H/J4CFIA== 7, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 23 -- To: {microscopy-at-microscopy.com} 7, 23 -- X-OriginalArrivalTime: 06 Oct 2008 15:51:56.0427 (UTC) FILETIME=[75C21DB0:01C927CB] 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m96Fq0Fh030522 ==============================End of - Headers==============================
It seems to me that there is a shortage of "advanced" biological EM techs out there. By advanced, I mean somebody that can come in and champion a project, maybe perform some Immunolabeling.
I know of three, possibly four labs looking and they are not getting very many applicants. I know one lab ran an ad through MSA and got two applicants. Is it a salary issue or is it a shortage of skilled workers? Combination of both?
What do labs do when they don't have the time to "groom" an employee?
Randy Nessler Associate Director Central Microscopy Research Facility University of Iowa Phone 319-335-8142
==============================Original Headers============================== 5, 23 -- From randy-nessler-at-uiowa.edu Mon Oct 6 10:52:46 2008 5, 23 -- Received: from hc-smtp2.healthcare.uiowa.edu (hc-smtp2.healthcare.uiowa.edu [129.255.210.33]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96FqjHd031120 5, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 10:52:46 -0500 5, 23 -- Received: from HC-MAIL11.healthcare.uiowa.edu ([129.255.112.31]) by hc-smtp2.healthcare.uiowa.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 23 -- Mon, 6 Oct 2008 10:52:42 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: Biological EM Techs 5, 23 -- Date: Mon, 6 Oct 2008 10:52:42 -0500 5, 23 -- Message-ID: {CBAD8B8185C05346A5B74AE1E12CBF2A037D94D1-at-HC-MAIL11.healthcare.uiowa.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: Biological EM Techs 5, 23 -- thread-index: Ackny5FXWFhYyJfdTSq8eToCN/ATJg== 5, 23 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 06 Oct 2008 15:52:42.0367 (UTC) FILETIME=[912400F0:01C927CB] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m96FqjHd031120 ==============================End of - Headers==============================
On Oct 6, 2008, at 9:02 AM, randy-nessler-at-uiowa.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It seems to me that there is a shortage of "advanced" biological EM } techs out there. By advanced, I mean somebody that can come in and } champion a project, maybe perform some Immunolabeling. } } I know of three, possibly four labs looking and they are not getting } very many applicants. I know one lab ran an ad through MSA and got two } applicants. Is it a salary issue or is it a shortage of skilled } workers? } Combination of both? } } What do labs do when they don't have the time to "groom" an employee?
OK, I'll bite. I don't know what the issues might be, but it's my job now to try and produce the kind of tech you are asking about.
What sort of things make an attractive EM tech these days? We have a fairly comprehensive curriculum, but it could always be improved.
What are the top skills that are needed by labs? How much do you want new employees to know vs what you would rather train them for on your terms?
How much photographic skill, Photoshop only or other things, how much vacuum experience, how about instrument operation and theory.
Do you want people who just know how to drive the bus or do you want them to know something about science as well?
I don't get out as much as I used too, I am counting on you all to help me know what it is like out there in real labs and enterprises.
Let me know what you value in a trained technician and I will try to include those ideas in our training program.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 16, 38 -- From jkrupp-at-deltacollege.edu Mon Oct 6 11:22:30 2008 16, 38 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 16, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96GMT7r025786 16, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 11:22:29 -0500 16, 38 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 16, 38 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 16, 38 -- with ESMTP id 42846401 for Microscopy-at-microscopy.com; Mon, 06 Oct 2008 09:22:28 -0700 16, 38 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 16, 38 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 16, 38 -- ESMTP id K8BRID00.KH6 for {Microscopy-at-microscopy.com} ; Mon, 6 16, 38 -- Oct 2008 09:08:37 -0700 16, 38 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 94E7A8F74DBD 16, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 09:22:28 -0700 (PDT) 16, 38 -- X-Virus-Scanned: amavisd-new at 16, 38 -- X-Spam-Flag: NO 16, 38 -- X-Spam-Score: -2.498 16, 38 -- X-Spam-Level: 16, 38 -- X-Spam-Status: No, score=-2.498 tagged_above=-10 required=6 tests=[AWL=0.001, 16, 38 -- BAYES_00=-2.599, RDNS_NONE=0.1] 16, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 16, 38 -- with ESMTP id lYy+t8ApGeyj for {Microscopy-at-microscopy.com} ; 16, 38 -- Mon, 6 Oct 2008 09:22:28 -0700 (PDT) 16, 38 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 1C0628F74DC3 16, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 09:22:28 -0700 (PDT) 16, 38 -- Message-Id: {E8BE9901-CBA6-478D-AFBF-016E55A47829-at-deltacollege.edu} 16, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 16, 38 -- To: Microscopy-at-microscopy.com 16, 38 -- In-Reply-To: {200810061602.m96G280f024158-at-ns.microscopy.com} 16, 38 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 16, 38 -- Content-Transfer-Encoding: 7bit 16, 38 -- Mime-Version: 1.0 (Apple Message framework v929.2) 16, 38 -- Subject: Re: [Microscopy] Biological EM Techs 16, 38 -- Date: Mon, 6 Oct 2008 09:22:27 -0700 16, 38 -- References: {200810061602.m96G280f024158-at-ns.microscopy.com} 16, 38 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
my take on this subject as someone who has been a tech for +20 years...
1) Salaries tend to be poor so not much incentive to stay in the field, expecially if you are married and have dependents. 2) Money for training/upgrading skills is usually non-existent or you have to do so much begging/paperwork/administration that its not worth it. 3) In fee-for-use facilities, a good deal of time is spent on accounting. Accountants make a lot more money so why not just become an accountant. 4) Universities gladly pay moving expenses for new faculty members but not technicians usually so little incentive to move. 5) University faculty promotion rates are far greater than the promotion rates for secretaries or technicians. 6) It takes time to train people. In this age of instant gratification, neither the facility nor university administrators will support anything that does not produce instant results and so fewer and fewer technicians are being developed. 7) EM Facilities are often seen by administrators as high cost, "old technology" facilities and a good place to save money through cutbacks. They often see little reason to keep "60's morphology" going when there are "new/exciting" research technologies to bring into the university.
When labs don't have the time to "groom" an employee, they run gels, PCR, and do sequence analysis.
Bitter ?!?! Not me.
B
It seems to me that there is a shortage of "advanced" biological EM techs out there. By advanced, I mean somebody that can come in and champion a project, maybe perform some Immunolabeling.
I know of three, possibly four labs looking and they are not getting very many applicants. I know one lab ran an ad through MSA and got two applicants. Is it a salary issue or is it a shortage of skilled workers? Combination of both?
What do labs do when they don't have the time to "groom" an employee?
Randy Nessler Associate Director Central Microscopy Research Facility University of Iowa Phone 319-335-8142
********************************************************************** Brent Gowen Electron Microscopy Laboratory Department of Biology University of Victoria P.O. Box 3020 STN CSC Victoria, BC, Canada V8W 3N5 Tel: (250)-721-7132 http://web.uvic.ca/em-lab/
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reznik-at-ict.uni-karlsruhe.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reznik-at-ict.uni-karlsruhe.de Name: Boris
Organization: University Karlsruhe
Title-Subject: [Filtered] soot_preparation
Question: Dear all users, we are looking for a preparation protocol of TEM samples of soot particles from diesel oil. A help is appreciate. Boris
I am about to use Aclar film to grow some cells which will eventually be embedded while still attached to the film (I can't use glass or plastic coverslips with the particular resin I intend to use). I can't remember what I did to sterilize the Aclar film in the old days when I last used it. I assumed we just autoclaved it but I found a dish labeled sterile with some pieces in it and they were all curled (presumably from autoclaving). Is there a way to avoid this? Any tips will be much appreciated. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
The American Society for Testing and Materials (ASTM International) has a standard method for the TEM characterization of carbon black. This protocol can also be used for the characterization of soot and even shows examples of diesel soot images in the published method. The protocol (ASTM D6602-03) is titled: "Standard Practice for Sampling and Testing of Possible Carbon Black Fugitive Emissions or Other Environmental Particulate, or Both." The method can be purchased at:
Brian R. Strohmeier, Ph.D. Corporate Laboratory Operations Manager Rj Lee Group, Inc. bstrohmeier-at-rjlg.com
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-----Original Message----- X-from: reznik-at-ict.uni-karlsruhe.de [mailto:reznik-at-ict.uni-karlsruhe.de] Sent: Monday, October 06, 2008 1:51 PM To: Brian Strohmeier
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both reznik-at-ict.uni-karlsruhe.de as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: reznik-at-ict.uni-karlsruhe.de Name: Boris
Organization: University Karlsruhe
Title-Subject: [Filtered] soot_preparation
Question: Dear all users, we are looking for a preparation protocol of TEM samples of soot particles from diesel oil. A help is appreciate. Boris
There are several ways to sterilize plastics. The best way (if you have the capability) is by ethylene oxide gas sterilization. Most hospitals have (or had) this capability. Next best is immersion in 70% ethanol for several hours, then air drying in a sterile petri dish. Third way: if you have a fume hood, generate some formaldehyde gas by heating paraformaldehyde on a hotplate and flowing it into a chamber (beaker) containing the Aclar strips. Stand up the Aclar (or put it on cotton) so that the gas contacts all surfaces. Takes about an hour. 4th way: germicidal UV lamp, 3-6 inches away for 30 minutes each side. 5th way: although I have not done it, put the strips in acidified 2% glutaraldehyde, 30 min at RT, rinse in sterile distilled water, air dry in sterile petri dish. 6th way: Immerse in Chlorox bleach for 15-20 min at RT, rinse in dwater, dry in sterile petri.
} I am about to use Aclar film to grow some cells which will eventually be } embedded while still attached to the film (I can't use glass or plastic } coverslips with the particular resin I intend to use). I can't remember } what I did to sterilize the Aclar film in the old days when I last used } it. I assumed we just autoclaved it but I found a dish labeled sterile } with some pieces in it and they were all curled (presumably from } autoclaving). Is there a way to avoid this? Any tips will be much } appreciated. Tom
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I don't have Photoshop, so I would have to defer to those who do. The idea of setting up one image as a reference to which all the others will be matched is absolutely correct. Others noted that the reference image could be made a layer in Photoshop and the image under repair could be stretched or shrunk until the scale bars matched. That is a valid approach, but as someone noted, you may need to be careful that the aspect ratio is not inadvertently changed.
Since the end result of such a process is changing the image size (or pixels per inch), I would recommend editing those values directly. Like I said, I don't have Photoshop, but most image programs offer the option to change DPI and print size. I favor FastStone Image Viewer for my personal use. It is robust enough for most applications and it is small enough that it loads quickly. It is NOT a PhotoShop replacement. And it has a nice batch mode where operations done on a single image can be applied to many images.
I used the resize/resample function within the program. I took as my task trying to match up images that were taken at 150x and 120x. I suppose you might also have a third or fourth magnification to reconcile.
The first issue would be to choose a magnification for the finished images. You can digitally magnify the images from lower a lower mag to a higher mag and the print size of the lower mag image will grow. You would then crop it back to the desired print size. (You could also demagnify the images taken at high magnification, but you would then have white space surrounding them compared to the other images taken at lower magnifications. You would end up cropping the low mag images as if you had magnified them in the first place.)
The next issue would be to determine the new parameters for the images. I have laid out my example calculations below.
Mag Res DPI Size Comment 150x 1024 227 4.51 150x original 120x 1024 227 4.51 120x original 150x' 1024 182 5.64 120x corrected to 150x
The only thing that changed was the print size and its attendant DPI. The existing number of pixels is stretched out to fit a wider image and DPI drops accordingly. I suppose you could redo the number of pixels at the same time, but that would be interpolating data. I would not do it unless pixels were becoming obvious in the print. Then I would increase the pixel count.
I would then crop the adjusted image back down to the same size as the high magnification images, e.g., 4.51 inches.
You didn't say how you were going to render or use the images. I inserted the images into Word and it dutifully checked the DPI and rendered the images at the proper sizes. The scale bars match up as intended. The same is true of PowerPoint.
The user needs to be careful not to sidestep the DPI rendering. If so, the same thing needs to be done to all images. For example, I tend to scale my images to fit the margins in Word. The images end up as 6.50 inches wide. I need to make sure I am stretching images from 4.51 inches to 6.50 inches and not stretching some from 4.51 inches and others from 5.64 inches. That would undo all the previous work.
I hope this helps.
Warren S.
-----Original Message----- X-from: rbeavers-at-mail.smu.edu [mailto:rbeavers-at-mail.smu.edu] Sent: Friday, October 03, 2008 1:28 PM To: wesaia-at-iastate.edu
Dear List,
I'm hoping maybe someone can offer a quick "I know that problem exactly and here is what to do" to get our sputter coater back up and running. As the subject says it is an Emitech K-550 (old model).
Problem: it isn't sputtering, voltage is under 5mA, but it jumps and spikes all over the place with arcs. The 4-5mA is obtained with the same setting we used to generate 20mA.
I first noticed exposed copper on the green wire going to the head (through the support arm). Took that apart, soldered it back together. Same problem.
I happened to wiggle the screw on BNC connector at the top of the sputter head and got a dramatic jump in voltage and found the connection to be failing. Although re-working that connector hasn't improved anything. (taking it off and testing it I would get no-connectivity, fixing it and the meter says it is fine).
That back on and it still isn't sputtering properly? Could the target be causing these problems? Anyone have any further suggestions?
It was one of those 'it was working great' then the next user 'it is broken' kind of situations.
TIA
Geoff Williams Leduc Bioimaging Facility Manager Brown University
Have you tried cleaning the insulator around the target head? It's made of Teflon, I believe, and should be mostly white. After a while it gets coated with whatever you're sputtering with and no longer insulates effectively. It is held on with screws and can be removed cleaned with acetone or ethanol and a good polishing, or just flipped upside down to use the other side. We had some extras made by our local instrument shop for when it becomes too coated to clean thoroughly, which entailed a set-up fee, but was much cheaper than ordering them from Emitech.
I don't know if this would greatly affect the mA value on the readout, but I do know that it can have a great effect on sputtering efficiency.
Service on these units is now handled by EMS, as I'm sure you know. They have done a great job for us.
Good luck, Randy
-----Original Message----- X-from: Geoffrey_Williams-at-brown.edu [mailto:Geoffrey_Williams-at-brown.edu] Sent: Monday, October 06, 2008 2:13 PM To: Tindall, Randy D.
Dear List,
I'm hoping maybe someone can offer a quick "I know that problem exactly and here is what to do" to get our sputter coater back up and running. As the subject says it is an Emitech K-550 (old model).
Problem: it isn't sputtering, voltage is under 5mA, but it jumps and spikes all over the place with arcs. The 4-5mA is obtained with the same setting we used to generate 20mA.
I first noticed exposed copper on the green wire going to the head (through the support arm). Took that apart, soldered it back together. Same problem.
I happened to wiggle the screw on BNC connector at the top of the sputter head and got a dramatic jump in voltage and found the connection to be failing. Although re-working that connector hasn't improved anything. (taking it off and testing it I would get no-connectivity, fixing it and the meter says it is fine).
That back on and it still isn't sputtering properly? Could the target be causing these problems? Anyone have any further suggestions?
It was one of those 'it was working great' then the next user 'it is broken' kind of situations.
TIA
Geoff Williams Leduc Bioimaging Facility Manager Brown University
Hi Tom I use 70% EtOH and then let the aclar air dry. VERY easy. David
On Oct 6, 2008, at 11:30 AM, PhillipsT-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I am about to use Aclar film to grow some cells which will } eventually be } embedded while still attached to the film (I can't use glass or } plastic } coverslips with the particular resin I intend to use). I can't } remember } what I did to sterilize the Aclar film in the old days when I last } used } it. I assumed we just autoclaved it but I found a dish labeled sterile } with some pieces in it and they were all curled (presumably from } autoclaving). Is there a way to avoid this? Any tips will be much } appreciated. Tom } } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Chair, MU Faculty Council } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } } } ==============================Original } Headers============================== } 8, 23 -- From PhillipsT-at-missouri.edu Mon Oct 6 13:24:34 2008 } 8, 23 -- Received: from um-nsmtpout1.um.umsystem.edu (um- } nsmtpout1.um.umsystem.edu [209.106.228.53]) } 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m96IOX0Y003097 } 8, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 13:24:34 } -0500 } 8, 23 -- Received: from UM-XMAIL06.um.umsystem.edu } ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 8, 23 -- Mon, 6 Oct 2008 13:24:32 -0500 } 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 8, 23 -- Content-class: urn:content-classes:message } 8, 23 -- MIME-Version: 1.0 } 8, 23 -- Content-Type: text/plain; } 8, 23 -- charset="us-ascii" } 8, 23 -- Subject: Aclar film } 8, 23 -- Date: Mon, 6 Oct 2008 13:24:30 -0500 } 8, 23 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD050FBB40-at-UM- } XMAIL06.um.umsystem.edu} } 8, 23 -- X-MS-Has-Attach: } 8, 23 -- X-MS-TNEF-Correlator: } 8, 23 -- Thread-Topic: Aclar film } 8, 23 -- Thread-Index: Ackn4MY5r5mJvD9yTxy3Ja1zs4Gvew== } 8, 23 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 8, 23 -- To: {Microscopy-at-microscopy.com} } 8, 23 -- X-OriginalArrivalTime: 06 Oct 2008 18:24:32.0305 (UTC) } FILETIME=[C7163A10:01C927E0] } 8, 23 -- Content-Transfer-Encoding: 8bit } 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m96IOX0Y003097 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Mon Oct 6 14:59:56 2008 5, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.arizona.edu [128.196.133.168]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96Jxt3g023543 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Mon, 6 Oct 2008 14:59:56 -0500 5, 22 -- Received: from frodos_amavis (amavis1.email.arizona.edu [10.0.0.204]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 47704540B6D 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Mon, 6 Oct 2008 12:59:52 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 62C7153F35D 5, 22 -- for {MICROSCOPY-at-ns.microscopy.com} ; Mon, 6 Oct 2008 12:59:50 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753.1) 5, 22 -- In-Reply-To: {200810061830.m96IUq1A008035-at-ns.microscopy.com} 5, 22 -- References: {200810061830.m96IUq1A008035-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {F771B6E8-6BE3-48F8-8FC4-91862E15DB3C-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] Aclar film 5, 22 -- Date: Mon, 6 Oct 2008 12:59:49 -0700 5, 22 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com} 5, 22 -- X-Mailer: Apple Mail (2.753.1) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Energy Beam Sciences is the US Master Distributor for the Polaron and Emitech line of equipment, located in East Granby, CT. We have a complete Equipment Service Dept., and you are welcome to contact Rob Diver (Service Technician) to review your K550 Sputter Coater with him. Rob can be reached at 800-992-9037 X361.
As for a quick check of the K550, with what you have done at present, you may want to check and clean with isopropanol, underneath and around the target head area, removing any metal contamination build-up that may have occurred. If there is metal contamination, you can have arcing, which can cause the voltage problems you described.
Let me know if this solves the problem. Regards,
Mike Dufraine Energy Beam Sciences, Inc.
Geoffrey_Williams-at-brown.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear List, } } I'm hoping maybe someone can offer a quick "I know that problem exactly and here is what to do" to get our sputter coater back up and running. As the subject says it is an Emitech K-550 (old model). } } Problem: it isn't sputtering, voltage is under 5mA, but it jumps and spikes all over the place with arcs. The 4-5mA is obtained with the same setting we used to generate 20mA. } } I first noticed exposed copper on the green wire going to the head (through the support arm). Took that apart, soldered it back together. Same problem. } } I happened to wiggle the screw on BNC connector at the top of the sputter head and got a dramatic jump in voltage and found the connection to be failing. Although re-working that connector hasn't improved anything. (taking it off and testing it I would get no-connectivity, fixing it and the meter says it is fine). } } That back on and it still isn't sputtering properly? Could the target be causing these problems? Anyone have any further suggestions? } } It was one of those 'it was working great' then the next user 'it is broken' kind of situations. } } TIA } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ } } } } ==============================Original Headers============================== } 11, 29 -- From Geoffrey_Williams-at-brown.edu Mon Oct 6 14:11:42 2008 } 11, 29 -- Received: from pyxis.services.brown.edu (pyxis.services.brown.edu [128.148.106.154]) } 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96JBf9B004807 } 11, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 14:11:41 -0500 } 11, 29 -- Received: from ex-gateway2-out.AD.Brown.Edu (ex-gateway2.ad.brown.edu [128.148.21.53]) } 11, 29 -- by pyxis.services.brown.edu (Switch-3.1.10/Switch-3.1.10) with ESMTP id m96JBefD020180 } 11, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 15:11:41 -0400 (EDT) } 11, 29 -- Received: from mail1.AD.Brown.Edu ([128.148.21.30]) by ex-gateway2-out.AD.Brown.Edu with Microsoft SMTPSVC(6.0.3790.1830); } 11, 29 -- Mon, 6 Oct 2008 15:11:40 -0400 } 11, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 11, 29 -- Content-class: urn:content-classes:message } 11, 29 -- MIME-Version: 1.0 } 11, 29 -- Content-Type: text/plain; } 11, 29 -- charset="iso-8859-1" } 11, 29 -- Subject: Emitech K-550 coater problem } 11, 29 -- Date: Mon, 6 Oct 2008 15:11:40 -0400 } 11, 29 -- Message-ID: {A1A84D541C161C4C988B4E0FB38F5A0F06F73A47-at-MAIL1.AD.Brown.Edu} } 11, 29 -- X-MS-Has-Attach: } 11, 29 -- X-MS-TNEF-Correlator: } 11, 29 -- Thread-Topic: Emitech K-550 coater problem } 11, 29 -- Thread-Index: Ackn51yQFHixCEXSQ9WZ3/EVRcMRNg== } 11, 29 -- From: "Williams, Geoffrey" {Geoffrey_Williams-at-brown.edu} } 11, 29 -- To: {Microscopy-at-microscopy.com} } 11, 29 -- X-OriginalArrivalTime: 06 Oct 2008 19:11:40.0789 (UTC) FILETIME=[5CFEA250:01C927E7] } 11, 29 -- X-Brown-Proofpoint: Not Infected } 11, 29 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0805090000 definitions=main-0810060139 } 11, 29 -- X-Brown-MailScanner-SpamCheck: not spam, rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0805090000 definitions=main-0810060139 } 11, 29 -- Content-Transfer-Encoding: 8bit } 11, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m96JBf9B004807 } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 12, 21 -- From mdufraine-at-ebsciences.com Mon Oct 6 15:00:17 2008 12, 21 -- Received: from mail.ebsciences.com (mail.ebsciences.com [65.115.7.18]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96K0G9x023795 12, 21 -- for {microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 15:00:17 -0500 12, 21 -- Received: from [10.10.0.112] 12, 21 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 12, 21 -- (Exim 4.69) 12, 21 -- (envelope-from {mdufraine-at-ebsciences.com} ) 12, 21 -- id 1KmwFb-0002Xo-3q; Mon, 06 Oct 2008 16:00:15 -0400 12, 21 -- Message-ID: {48EA6E4D.50403-at-ebsciences.com} 12, 21 -- Date: Mon, 06 Oct 2008 16:00:13 -0400 12, 21 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 12, 21 -- Organization: Energy Beam Sciences 12, 21 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 12, 21 -- MIME-Version: 1.0 12, 21 -- To: Geoffrey_Williams-at-brown.edu, microscopy-at-microscopy.com 12, 21 -- Subject: Re: [Microscopy] Emitech K-550 coater problem 12, 21 -- References: {200810061912.m96JCHXs006776-at-ns.microscopy.com} 12, 21 -- In-Reply-To: {200810061912.m96JCHXs006776-at-ns.microscopy.com} 12, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
In response to the suggestions: When I had it all apart I cleaned the Teflon insulator as best as I could (nearly white now, instead of well coated), the ohm meter is showing the target shield to be completely insulated from the lid.
Short version of what follows: I think it is fixed.
Long version: Between sending the email out and now, the one thing that was bothering me was the lack of connectivity to the Au/Pd target. So I checked it again and noticed a gap or two around the edge. Hmm. Oops next thing I know the film detaches from the backing. It seems to have been held in with a clear looking 'glue.' I put a bit of carbon adhesive on there and seemed to get not quite zero Ohms resistance but pretty close. And after letting it pump for a good 30-40 minute (on hold), it started sputtering perfectly.
This led me to guess that the wires and the sketchy connections might have had nothing to do with the "problem" in the first place. So... Sorry to clog emails with this big of head-scratching.
Geoff Williams Leduc Bioimaging Facility Manager Brown University
http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ -----Original Message----- X-from: Tindall, Randy D. [mailto:TindallR-at-missouri.edu] Sent: Monday, October 06, 2008 3:38 PM To: Williams, Geoffrey Cc: microscopy-at-microscopy.com
Dear List,
I'm hoping maybe someone can offer a quick "I know that problem exactly and here is what to do" to get our sputter coater back up and running. As the subject says it is an Emitech K-550 (old model).
Problem: it isn't sputtering, voltage is under 5mA, but it jumps and spikes all over the place with arcs. The 4-5mA is obtained with the same setting we used to generate 20mA.
I first noticed exposed copper on the green wire going to the head (through the support arm). Took that apart, soldered it back together. Same problem.
I happened to wiggle the screw on BNC connector at the top of the sputter head and got a dramatic jump in voltage and found the connection to be failing. Although re-working that connector hasn't improved anything. (taking it off and testing it I would get no-connectivity, fixing it and the meter says it is fine).
That back on and it still isn't sputtering properly? Could the target be causing these problems? Anyone have any further suggestions?
It was one of those 'it was working great' then the next user 'it is broken' kind of situations.
TIA
Geoff Williams Leduc Bioimaging Facility Manager Brown University
I've encountered similar issues with other brand coaters. First, clean the head and target holder/support well. Deposition of sputtered metal there over time will cause shorts. Second, check the feed-throughs in the top of the coater. There likely is high-vacuum epoxy there, and it can break down over time. This can be resealed with more high-vacuum epoxy, but be careful not to make things worse. Frustrating, that is. If it's not either of these, it's most likely one or more of the wires or connections.
Phil
} Dear List, } } I'm hoping maybe someone can offer a quick "I } know that problem exactly and here is what to } do" to get our sputter coater back up and } running. As the subject says it is an Emitech } K-550 (old model). } } Problem: it isn't sputtering, voltage is under } 5mA, but it jumps and spikes all over the place } with arcs. The 4-5mA is obtained with the same } setting we used to generate 20mA. } } I first noticed exposed copper on the green wire } going to the head (through the support arm). } Took that apart, soldered it back together. } Same problem. } } I happened to wiggle the screw on BNC connector } at the top of the sputter head and got a } dramatic jump in voltage and found the } connection to be failing. Although re-working } that connector hasn't improved anything. } (taking it off and testing it I would get } no-connectivity, fixing it and the meter says it } is fine). } } That back on and it still isn't sputtering } properly? Could the target be causing these } problems? Anyone have any further suggestions? } } It was one of those 'it was working great' then } the next user 'it is broken' kind of situations. } } TIA } } Geoff Williams } Leduc Bioimaging Facility Manager } Brown University } Ý } http://www.brown.edu/Facilities/Leduc_Bioimaging_Facility/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 28 -- From oshel1pe-at-cmich.edu Mon Oct 6 15:07:41 2008 5, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96K7ePw011686 5, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 15:07:40 -0500 5, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 28 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m96K7UEd011522; 5, 28 -- Mon, 6 Oct 2008 16:07:36 -0400 5, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 5, 28 -- Mon, 6 Oct 2008 16:07:06 -0400 5, 28 -- Mime-Version: 1.0 5, 28 -- Message-Id: {f06240817c5101fa9b8c0-at-[141.209.160.249]} 5, 28 -- In-Reply-To: {200810061917.m96JH3Kq019414-at-ns.microscopy.com} 5, 28 -- References: {200810061917.m96JH3Kq019414-at-ns.microscopy.com} 5, 28 -- Date: Mon, 6 Oct 2008 16:07:04 -0400 5, 28 -- To: Geoffrey_Williams-at-brown.edu 5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 28 -- Subject: Re: [Microscopy] Emitech K-550 coater problem 5, 28 -- Cc: Microscopy-at-microscopy.com 5, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 5, 28 -- X-OriginalArrivalTime: 06 Oct 2008 20:07:06.0137 (UTC) FILETIME=[1B0E7890:01C927EF] 5, 28 -- X-Canit-CHI2: 0.00 5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 28 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 5, 28 -- X-CanItPRO-Stream: default 5, 28 -- X-Canit-Stats-ID: 3565875 - 9871b6da5b87 5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m96K7ePw011686 ==============================End of - Headers==============================
Dear Colleagues You are cordially invited to submit abstracts to the MRS Spring 2008 meeting Symposium JJ on Nanoscale Electromechanics and Piezoresponse Force Microscopy. The symposium will feature recent advances in experimental methods and theoretical understanding of electromechanical coupling on the nanoscale in polar, ferroelectric, macromolecular, and biological systems. The goal of this symposium is to bring together the experts from biology, materials science, and organic chemistry communities interested in electromechanical processes, scanning probe microscopists developing the experimental pathway for probing nanoelectromechanics, and theorists interested in fundamental mechanisms of electromechanical energy conversion, to formulate the outstanding research needs, grand challenges, applications, and development pathway for this rapidly emerging field. The symposium information is available on line at http://www.mrs.org/s_mrs/sec.asp?CID=14465&DID=211517
The information on the PFM and materials of PFM workshops can be found in the section "Knowledge Base" at http://imaging.ornl.gov
Looking forward to seeing you in San Francisco Sergei V. Kalinin
==============================Original Headers============================== 3, 24 -- From sergei2-at-ornl.gov Mon Oct 6 17:32:21 2008 3, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m96MWLsF019591 3, 24 -- for {microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 17:32:21 -0500 3, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 3, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 3, 24 -- with ESMTP id {0K8C00H2A99UV7-at-emroute1.ornl.gov} for 3, 24 -- microscopy-at-microscopy.com; Mon, 06 Oct 2008 18:32:18 -0400 (EDT) 3, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 3, 24 -- (PMDF V6.4 #31561) id {0K8C00I0199UUH-at-emroute1.ornl.gov} for 3, 24 -- microscopy-at-microscopy.com; Mon, 06 Oct 2008 18:32:18 -0400 (EDT) 3, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 3, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 3, 24 -- with ESMTP id {0K8C004GH99U62-at-emroute1.ornl.gov} for 3, 24 -- microscopy-at-microscopy.com; Mon, 06 Oct 2008 18:32:18 -0400 (EDT) 3, 24 -- Date: Mon, 06 Oct 2008 18:32:18 -0400 3, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 3, 24 -- Subject: MRS 2008 Spring Meeting - Piezoresponse Force Microscopy 3, 24 -- To: microscopy-at-microscopy.com, "Sergei V. Kalinin" {sergei2-at-ornl.gov} 3, 24 -- Message-id: {48EA91F2.6000102-at-ornl.gov} 3, 24 -- MIME-version: 1.0 3, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 3, 24 -- Content-transfer-encoding: 7bit 3, 24 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) ==============================End of - Headers==============================
Another easy sterilisation method is to place items to be sterilised in a container - an old dessicator works fine, add a beaker containing 100 ml standard bleach (from the shop), add 3 ml conc HCl to the bleach, quickly place lid on desiccator. This will sterilise seeds in 2-3 hours, should sterilise something like film in a shorter time.
cheers, Rosemary. ________________________________________ X-from: bozzola-at-siu.edu [bozzola-at-siu.edu] Sent: Tuesday, October 07, 2008 6:23 AM To: White, Rosemary (PI, Black Mountain)
There are several ways to sterilize plastics. The best way (if you have the capability) is by ethylene oxide gas sterilization. Most hospitals have (or had) this capability. Next best is immersion in 70% ethanol for several hours, then air drying in a sterile petri dish. Third way: if you have a fume hood, generate some formaldehyde gas by heating paraformaldehyde on a hotplate and flowing it into a chamber (beaker) containing the Aclar strips. Stand up the Aclar (or put it on cotton) so that the gas contacts all surfaces. Takes about an hour. 4th way: germicidal UV lamp, 3-6 inches away for 30 minutes each side. 5th way: although I have not done it, put the strips in acidified 2% glutaraldehyde, 30 min at RT, rinse in sterile distilled water, air dry in sterile petri dish. 6th way: Immerse in Chlorox bleach for 15-20 min at RT, rinse in dwater, dry in sterile petri.
} I am about to use Aclar film to grow some cells which will eventually be } embedded while still attached to the film (I can't use glass or plastic } coverslips with the particular resin I intend to use). I can't remember } what I did to sterilize the Aclar film in the old days when I last used } it. I assumed we just autoclaved it but I found a dish labeled sterile } with some pieces in it and they were all curled (presumably from } autoclaving). Is there a way to avoid this? Any tips will be much } appreciated. Tom
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: Scott.Streiker-at-udri.udayton.edu Name: Scott Streiker
Organization: University of Dayton Research Institute
Title-Subject: [Filtered] what kind of resolution can be obtained with various microscopies
Question: On behalf of Chair, University Dayton Chemistry Department please respond to the following question by listserver posting and cc: by e-mail. Thanks,
"I am basically wondering what kind of resolution can be obtained with various microscopies (TEM, SEM, AFM, STM) with regard to noting changes in the small (5-10 Angstrom) and large (up to 500 Angstrom) ranges. I believe that our samples, which consist of ~45A thick "purple" membrane - which is tightly packed (~50% of membrane surface area) with the purple protein bacteriorhodopsin (BR; 26 kDa, comprised of 7 alpha-helices arranged in a circle with the long axes of the helices perpendicular to the plane of the membrane). The BR molecules are organized into a hexagonal lattice of trimers, with distances of ~60A separating the center of adjacent pairs of trimers (see attached "Protein Membranes..." figure).
We believe that when we induce a purple-to-blue color change with green (532 nm) laser pulses (see attached "Composite..." figure" that ~1/3 of the molecules (~1 molecule per trimer) are converted to a colorless species, and that this damaged molecule induces conformational changes in BR molecules in its own trimer or nearby trimers which lead to the color change (see attached "Correct Protein ..." figure. We are interested in knowing if we would be able to detect such conformational changes via any of the microscopies listed above. We would need to be able to look at the membrane at both the large and small scales listed above to determine if the membrane becomes distorted in and/or out of the membrane plane."
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Title-Subject: [Filtered] Textile types and Analysis Methods
Question:
Hello All,
I am wondering if anyone has micrographs of various textiles types and analysis methods for distinguishing between different matrices and fiber types of wool, cotton, linen and various synthetic materials? Any direct input or referral would be appreciated.
Thanks,
Jeanette Vass Senior Analytical Chemist Brandywine Research Laboratory 60 Blue Hen Drive Newark, DE 19713 jeanette-at-corrosionlab.com |--mailto:jeanette-at-corrosionlab.com--| web: www.corrosionlab.com |--http://www.corrosionlab.com--| voice: 302-454-8200 fax: 302-454-8204
Atlas of Fibre Fracture and Damage to Textiles, Second Edition (Hardcover) by John W. S. Hearle, Brenda Lomas, William D. Cooke, Published by CRC, ISBN: 0849338816
It contains plenty of electron microscope images of different textile material and also of their failure modes (see title :).
Best regards,
Petra __________________________________ Dr. Petra Wahlbring Lead Engineer Analytical Test Lab GTC*L Colmar-Berg, Luxembourg + petra.wahlbring-at-goodyear.com ( +352 8199 3725 or GTN 631 3725 6 +352 8199 5643 - May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the express written consent of The Goodyear Tire & Rubber Company. -
jeanette-at-corrosio nlab.com To 10/07/08 02:18 AM petra.wahlbring-at-goodyear.com cc
Please respond to Subject jeanette-at-corrosio [Microscopy] viaWWW: Textile types nlab.com and Analysis Methods
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Title-Subject: [Filtered] Textile types and Analysis Methods
Question:
Hello All,
I am wondering if anyone has micrographs of various textiles types and analysis methods for distinguishing between different matrices and fiber types of wool, cotton, linen and various synthetic materials? Any direct input or referral would be appreciated.
Thanks,
Jeanette Vass Senior Analytical Chemist Brandywine Research Laboratory 60 Blue Hen Drive Newark, DE 19713 jeanette-at-corrosionlab.com |--mailto:jeanette-at-corrosionlab.com--| web: www.corrosionlab.com |--http://www.corrosionlab.com--| voice: 302-454-8200 fax: 302-454-8204
Dear Erman It might be worth putting the ion pump through a cryo cycle overnight. It could be degassing if it over saturated.
On Wed, Sep 24, 2008 at 3:45 AM, {bengu-at-fen.bilkent.edu.tr} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both bengu-at-fen.bilkent.edu.tr as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: bengu-at-fen.bilkent.edu.tr } Name: Erman Bengu } } Organization: Bilkent University } } Title-Subject: [Filtered] [TEM] Vacuum Issue } } Question: } There has been a sudden rise for current reading on the ion gun for } the FEG region for our Tecnai. It is fluctuating between 1-2 uA. I } think the maximum allowable value is around 0.5 uA. During the time } frame this problem occurred, equipment was idle for about a week. So, } I have no idea what migth be the root cause for this issue. Any } ideas, comments? } } Thanx } } Erman Bengu } } Login Host: 139.179.158.12 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Sep 23 20:36:22 2008 } 8, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m8O1aLod002953 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 23 Sep 2008 20:36:22 -0500 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240805c4ff49fb8e3a-at-[206.69.208.22]} } 8, 11 -- Date: Tue, 23 Sep 2008 20:36:11 -0500 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: bengu-at-fen.bilkent.edu.tr (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: [TEM] Vacuum Issue } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
-- Stephan H Coetzee Chief Technician Electron Microscope Unit University of Botswana
Hello Jeanette, I would recomend polarized light microscopy. Unfortunately I have none of my references in my new position as a metallurist. I would suggest McCrone's Particle Atlas for a starting place. I understand it is available on CD.
Stay safe...... Frank Karl..... Microscopy Group Manager, Linked-In
jeanette-at-corrosio nlab.com To 10/06/2008 08:21 frank_karl-at-lincolnelectric.com PM cc
Subject Please respond to [Microscopy] viaWWW: Textile types jeanette-at-corrosio and Analysis Methods nlab.com
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Title-Subject: [Filtered] Textile types and Analysis Methods
Question:
Hello All,
I am wondering if anyone has micrographs of various textiles types and analysis methods for distinguishing between different matrices and fiber types of wool, cotton, linen and various synthetic materials? Any direct input or referral would be appreciated.
Thanks,
Jeanette Vass Senior Analytical Chemist Brandywine Research Laboratory 60 Blue Hen Drive Newark, DE 19713 jeanette-at-corrosionlab.com |--mailto:jeanette-at-corrosionlab.com--| web: www.corrosionlab.com |--http://www.corrosionlab.com--| voice: 302-454-8200 fax: 302-454-8204
==============================Original Headers============================== 13, 11 -- From zaluzec-at-microscopy.com Mon Oct 6 19:16:04 2008 13, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 13, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m970G2o4022698 13, 11 -- for {microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 19:16:03 -0500 13, 11 -- Mime-Version: 1.0 13, 11 -- Message-Id: {p06240801c5105aaf71b1-at-[206.69.208.22]} 13, 11 -- Date: Mon, 6 Oct 2008 19:16:02 -0500 13, 11 -- To: microscopy-at-microscopy.com 13, 11 -- From: jeanette-at-corrosionlab.com (by way of MicroscopyListserver) 13, 11 -- Subject: viaWWW: Textile types and Analysis Methods 13, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 32, 22 -- From frank_karl-at-lincolnelectric.com Tue Oct 7 06:03:05 2008 32, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 32, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m97B34sS003924 32, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Oct 2008 06:03:04 -0500 32, 22 -- In-Reply-To: {200810070021.m970LmHL009918-at-ns.microscopy.com} 32, 22 -- Subject: Re: [Microscopy] viaWWW: Textile types and Analysis Methods 32, 22 -- To: jeanette-at-corrosionlab.com, Microscopy-at-microscopy.com 32, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 32, 22 -- Message-ID: {OF7CCD2011.F6B70CA7-ON852574DB.003BFF79-852574DB.003CA363-at-lincolnelectric.com} 32, 22 -- Date: Tue, 7 Oct 2008 07:03:03 -0400 32, 22 -- From: Frank_Karl-at-lincolnelectric.com 32, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 10/07/2008 07:02:53 AM, 32, 22 -- CD-MIME complete at 10/07/2008 07:02:53 AM, 32, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 10/07/2008 07:02:53 AM, 32, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 32, 22 -- 07, 2008) at 10/07/2008 07:02:53 AM, 32, 22 -- Serialize complete at 10/07/2008 07:02:53 AM 32, 22 -- MIME-Version: 1.0 32, 22 -- Content-Type: text/plain; 32, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bjulies-at-uwc.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bjulies-at-uwc.ac.za Name: Basil Julies
Organization: University of the Western Cape
Title-Subject: [Filtered] Tecnai F20 FEG TEM operator-senior lecturer post
Question: Dear All
This is a final advertisement for the permanent post of senior lecturer with the operation of the new Tecnai F20 being the main responsibility before an appointment will be made. Knowledge or experience of EELS will be a deciding factor. The University of the Western Cape (UWC) is based in Cape Town, South Africa (the country where the 2010 Football World Cup will be taking place). If you are interested and wish to know more, please reply to me (Basil) directly. All CV's and coverings letters must be sent to me as well. Closing date: Friday 24 October 2008.
Kind regards
Basil Head: Electron Microscope Unit, UWC Tel: +27 21 959 2327 Fax: +27 21 959 1335
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mdyousuf-at-qu.edu.qa as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mdyousuf-at-qu.edu.qa Name: Mohammed Yousuf
Organization: Qatar university, Doha-Qatar
Title-Subject: [Filtered] Need guidance for ISO17025 accreditation (SEM-EDS)
Question: Dear all, My higher ups are pressing us to go in for ISO17025 accreditation. In my operational control I have one SEM equipped with an EDS (EDAX) attachment; a FEI Quanta200. In what ways, and what methods, I can go in for certification? The other equipments in our lab, like the Atomic Absorption Spectrometers, the ICP-MS, the GCs have defined methodologies and SOPs, and hence can readly go for certification. Please advise, if some of you have gone through these procedures of EDS certification for ISO 17025. I would appreciate your valuable input.
*Are imaging applications also covered by ISO 17025?
Mohammed Yousuf Senior Researcher Electron Microscopy Lab Central Laboratories Qatar University POB 2713 Doha, Qatar +974-5720907 +974-4852145 mdyousuf-at-qu.edu.qa
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arnec-at-bio.umass.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arnec-at-bio.umass.edu Name: Arne Christensen
Organization: Conte Andadromous Fish Lab
Title-Subject: [Filtered] JEOL 5800LV differential pumping unit
Question: Our laboratory has recently acquired a used JEOL JSM-5800LV SEM. We had a JEOL technician survey the equipment to assess its condition. Apparently the SEM is missing a Differential Pumping Unit, and without a DPU the machine can not be installed.
Can anyone provide any information as where we might acquire a DPU that is compatible with the JSM-5800LV?
If you happen to have access to a "Plasma Cleaner" (often used for making carbon films wettable) they can be used for sterilization. Just put the films in petri dishes - the field (and plasma penetrate inside the containers...) and treat (see references).
I have no connection with Harrick except being a happy user and found their info easily accessible...
Dale
bozzola-at-siu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } There are several ways to sterilize plastics. The best way (if you } have the capability) is by ethylene oxide gas sterilization. Most } hospitals have (or had) this capability. Next best is immersion in } 70% ethanol for several hours, then air drying in a sterile petri } dish. Third way: if you have a fume hood, generate some formaldehyde } gas by heating paraformaldehyde on a hotplate and flowing it into a } chamber (beaker) containing the Aclar strips. Stand up the Aclar (or } put it on cotton) so that the gas contacts all surfaces. Takes about } an hour. 4th way: germicidal UV lamp, 3-6 inches away for 30 minutes } each side. 5th way: although I have not done it, put the strips in } acidified 2% glutaraldehyde, 30 min at RT, rinse in sterile distilled } water, air dry in sterile petri dish. 6th way: Immerse in Chlorox } bleach for 15-20 min at RT, rinse in dwater, dry in sterile petri. } } } } I am about to use Aclar film to grow some cells which will eventually be } } embedded while still attached to the film (I can't use glass or plastic } } coverslips with the particular resin I intend to use). I can't remember } } what I did to sterilize the Aclar film in the old days when I last used } } it. I assumed we just autoclaved it but I found a dish labeled sterile } } with some pieces in it and they were all curled (presumably from } } autoclaving). Is there a way to avoid this? Any tips will be much } } appreciated. Tom }
==============================Original Headers============================== 5, 22 -- From dac-at-research.umass.edu Tue Oct 7 12:52:59 2008 5, 22 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m97HqxqF015165 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Oct 2008 12:52:59 -0500 5, 22 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 5, 22 -- (authenticated bits=0) 5, 22 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id m97HquEo010374 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Oct 2008 13:52:56 -0400 5, 22 -- Message-ID: {48EBB032.3090209-at-research.umass.edu} 5, 22 -- Date: Tue, 07 Oct 2008 13:53:38 -0500 5, 22 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 22 -- Reply-To: dac-at-research.umass.edu 5, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.16) Gecko/20080702 SeaMonkey/1.1.11 5, 22 -- MIME-Version: 1.0 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] Re: Aclar film 5, 22 -- References: {200810061920.m96JKXPT025992-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {200810061920.m96JKXPT025992-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Scott Stoeffler, a member of our Light Microscopy Group, has provided some resource information:
PLM is definitely the way to go for most fiber ID, supplemented in some cases by micro FTIR. Introductory Textile Science by Marjory Joseph is a good basic book on fibers and textiles, but not so heavy on ID methods. Microscopy of Textile Fibres by Greaves and Saville and Forensic Examination of Fibres by Robertson and Grieve are better references for ID methods. Identification of Textile Materials from the Textile Institute and The Identification of Textile Fibres by Luniak are also good ID texts, through they are out of print and not up to date on some of the more modern fiber types. The fiber fracture and damage atlas deals more with identifying failure modes than fiber and textile types.
Also, in addition to the CD version of the Particle Atlas, the online version can be found at www.mccroneatlas.com. Micrographs and basic particle descriptions can be viewed at no cost, and subscription information is available on the website.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
-----Original Message----- X-from: Frank_Karl-at-lincolnelectric.com [mailto:Frank_Karl-at-lincolnelectric.com] Sent: Tuesday, October 07, 2008 6:14 AM To: Elaine F. Schumacher
Hello Jeanette, I would recomend polarized light microscopy. Unfortunately I have none of my references in my new position as a metallurist. I would suggest McCrone's Particle Atlas for a starting place. I understand it is available on CD.
Stay safe...... Frank Karl..... Microscopy Group Manager, Linked-In
jeanette-at-corrosio
nlab.com
To 10/06/2008 08:21 frank_karl-at-lincolnelectric.com
PM cc
Subject Please respond to [Microscopy] viaWWW: Textile types jeanette-at-corrosio and Analysis Methods
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both jeanette-at-corrosionlab.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] Textile types and Analysis Methods
Question:
Hello All,
I am wondering if anyone has micrographs of various textiles types and analysis methods for distinguishing between different matrices and fiber types of wool, cotton, linen and various synthetic materials? Any direct input or referral would be appreciated.
Thanks,
Jeanette Vass Senior Analytical Chemist Brandywine Research Laboratory 60 Blue Hen Drive Newark, DE 19713 jeanette-at-corrosionlab.com |--mailto:jeanette-at-corrosionlab.com--| web: www.corrosionlab.com |--http://www.corrosionlab.com--| voice: 302-454-8200 fax: 302-454-8204
==============================Original Headers============================== 55, 24 -- From eschumacher-at-mccrone.com Tue Oct 7 15:41:08 2008 55, 24 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 55, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m97Kf83u004812 55, 24 -- for {microscopy-at-microscopy.com} ; Tue, 7 Oct 2008 15:41:08 -0500 55, 24 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 55, 24 -- Tue, 7 Oct 2008 15:41:07 -0500 55, 24 -- Content-class: urn:content-classes:message 55, 24 -- MIME-Version: 1.0 55, 24 -- Content-Type: text/plain; 55, 24 -- charset="US-ASCII" 55, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 55, 24 -- Subject: RE: [Microscopy] Re: viaWWW: Textile types and Analysis Methods 55, 24 -- Date: Tue, 7 Oct 2008 15:41:02 -0500 55, 24 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150E15E-at-MCCRONEMSG.tmg.mccrone.com} 55, 24 -- In-Reply-To: {200810071114.m97BEL8D017191-at-ns.microscopy.com} 55, 24 -- X-MS-Has-Attach: 55, 24 -- X-MS-TNEF-Correlator: 55, 24 -- Thread-Topic: [Microscopy] Re: viaWWW: Textile types and Analysis Methods 55, 24 -- Thread-Index: AckobdlgJW9MndauQ+CL66ooYgwMEwATlN1Q 55, 24 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 55, 24 -- To: {microscopy-at-microscopy.com} 55, 24 -- X-OriginalArrivalTime: 07 Oct 2008 20:41:07.0851 (UTC) FILETIME=[066D0DB0:01C928BD] 55, 24 -- Content-Transfer-Encoding: 8bit 55, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m97Kf83u004812 ==============================End of - Headers==============================
On Oct 6, 2008, at 5:12 PM, Scott.Streiker-at-udri.udayton.edu wrote:
} Name: Scott Streiker } } Organization: University of Dayton Research Institute } } Title-Subject: [Filtered] what kind of resolution can be obtained } with various microscopies } } "I am basically wondering what kind of resolution can be obtained } with various microscopies (TEM, SEM, AFM, STM) with regard to noting } changes in the small (5-10 Angstrom) and large (up to 500 Angstrom) } ranges. I believe that our samples, which consist of ~45A thick } "purple" membrane - which is tightly packed (~50% of membrane surface } area) with the purple protein bacteriorhodopsin (BR; 26 kDa, } comprised of 7 alpha-helices arranged in a circle with the long axes } of the helices perpendicular to the plane of the membrane). The BR } molecules are organized into a hexagonal lattice of trimers, with } distances of ~60A separating the center of adjacent pairs of trimers } (see attached "Protein Membranes..." figure). } } We believe that when we induce a purple-to-blue color change with } green (532 nm) laser pulses (see attached "Composite..." figure" that } ~1/3 of the molecules (~1 molecule per trimer) are converted to a } colorless species, and that this damaged molecule induces } conformational changes in BR molecules in its own trimer or nearby } trimers which lead to the color change (see attached "Correct Protein } ..." figure. We are interested in knowing if we would be able to } detect such conformational changes via any of the microscopies listed } above. We would need to be able to look at the membrane at both the } large and small scales listed above to determine if the membrane } becomes distorted in and/or out of the membrane plane." } Dear Scott, Since no one else has replied, I thought I'd have a go. I can say a little about TEM, but can only give wild guesses about the other techniques. Since the figures were not attached, I'll have to guess about them as well. TEM is inherently capable of collecting images in the resolution ranges you want, but there is a very big caveat: biological specimens are very radiation sensitive, and even more so when in an aqueous environment, so if your membrane is wet--for example, enclosed in an environmental chamber--the dose it can tolerate will be so low that the noise in the image will smear out detail at least in the 0.5-1 nm range. On the plus side, you could either take diffraction patterns or FFTs of your images (or both) and use the idea that each molecule that changes will do so in the same way to get an averaged image, which will have the information about the laser-induced changes. It will require a fair amount of image processing to tease apart the unchanged trimers from the changed ones, and if the change is not into one or a few distinct states, you won't be able to characterize it (but you may be able to locate the altered trimers). You could tilt the specimen to observe out-of-plane changes, but you will need to have very flat membranes so that each part of the image has the same orientation. If you can cause the changes in a membrane that is embedded in a thin film of water, then plunge-freeze the grid while maintaining the changes, you will get a considerable improvement in radiation sensitivity by using cryoTEM, but you don't say whether the laser induces transient or permanent changes. In vivo, BR is supposed to absorb light, change conformation to pump a proton across the membrane, then return to its initial state, but I don't know whether this is true in your preparation. I do not think that SEM will be useful, since you will not likely be able to coat your specimen, and the thin, low Z specimen will produce very little detectable signal, especially compared to the substrate. AFM and STM can be used in an aqueous environment, and it is likely that the membranes can be placed on a suitable substrate and imaged. I do not know what the resolution limits of these techniques are, and they only image surfaces, so if the changes do not alter the topography, they will not be observed (unless there is some change in the stiffness of the membrane, which could be detected in some AFM modes). Any real experts in these techniques will be able to give you much more authoritative information. Good luck. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Oct 7 18:42:14 2008 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m97NgD9o024230 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Oct 2008 18:42:14 -0500 4, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 4, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 768CE66E0919; 4, 22 -- Tue, 7 Oct 2008 16:42:13 -0700 (PDT) 4, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 4, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 4, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 1408466E0AA6; 4, 22 -- Tue, 7 Oct 2008 16:42:11 -0700 (PDT) 4, 22 -- Message-Id: {BD298588-FF47-4697-A971-C94C14C4A581-at-caltech.edu} 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- To: Scott.Streiker-at-udri.udayton.edu, microscopy-at-microscopy.com 4, 22 -- In-Reply-To: {200810070012.m970CNSG016237-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 4, 22 -- Subject: Re: [Microscopy] viaWWW: what kind of resolution can be obtained with various 4, 22 -- Date: Tue, 7 Oct 2008 16:42:11 -0700 4, 22 -- References: {200810070012.m970CNSG016237-at-ns.microscopy.com} 4, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Just a comment on the low tech end. Confocal - hydrated often live samples. EM fixed dehydrated in most cases. The biggest impact will be from the sample preparation. But TEM with the right sample prep might be the way to go linked with image 3d reconstruction. I will leave the rest to the experts.
On Wed, Oct 8, 2008 at 1:48 AM, {tivol-at-caltech.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Oct 6, 2008, at 5:12 PM, Scott.Streiker-at-udri.udayton.edu wrote: } } } Name: Scott Streiker } } } } Organization: University of Dayton Research Institute } } } } Title-Subject: [Filtered] what kind of resolution can be obtained } } with various microscopies } } } } "I am basically wondering what kind of resolution can be obtained } } with various microscopies (TEM, SEM, AFM, STM) with regard to noting } } changes in the small (5-10 Angstrom) and large (up to 500 Angstrom) } } ranges. I believe that our samples, which consist of ~45A thick } } "purple" membrane - which is tightly packed (~50% of membrane surface } } area) with the purple protein bacteriorhodopsin (BR; 26 kDa, } } comprised of 7 alpha-helices arranged in a circle with the long axes } } of the helices perpendicular to the plane of the membrane). The BR } } molecules are organized into a hexagonal lattice of trimers, with } } distances of ~60A separating the center of adjacent pairs of trimers } } (see attached "Protein Membranes..." figure). } } } } We believe that when we induce a purple-to-blue color change with } } green (532 nm) laser pulses (see attached "Composite..." figure" that } } ~1/3 of the molecules (~1 molecule per trimer) are converted to a } } colorless species, and that this damaged molecule induces } } conformational changes in BR molecules in its own trimer or nearby } } trimers which lead to the color change (see attached "Correct Protein } } ..." figure. We are interested in knowing if we would be able to } } detect such conformational changes via any of the microscopies listed } } above. We would need to be able to look at the membrane at both the } } large and small scales listed above to determine if the membrane } } becomes distorted in and/or out of the membrane plane." } } } Dear Scott, } Since no one else has replied, I thought I'd have a go. I can say a } little about TEM, but can only give wild guesses about the other } techniques. Since the figures were not attached, I'll have to guess } about them as well. TEM is inherently capable of collecting images in } the resolution ranges you want, but there is a very big caveat: } biological specimens are very radiation sensitive, and even more so } when in an aqueous environment, so if your membrane is wet--for } example, enclosed in an environmental chamber--the dose it can } tolerate will be so low that the noise in the image will smear out } detail at least in the 0.5-1 nm range. On the plus side, you could } either take diffraction patterns or FFTs of your images (or both) and } use the idea that each molecule that changes will do so in the same } way to get an averaged image, which will have the information about } the laser-induced changes. It will require a fair amount of image } processing to tease apart the unchanged trimers from the changed ones, } and if the change is not into one or a few distinct states, you won't } be able to characterize it (but you may be able to locate the altered } trimers). You could tilt the specimen to observe out-of-plane } changes, but you will need to have very flat membranes so that each } part of the image has the same orientation. If you can cause the } changes in a membrane that is embedded in a thin film of water, then } plunge-freeze the grid while maintaining the changes, you will get a } considerable improvement in radiation sensitivity by using cryoTEM, } but you don't say whether the laser induces transient or permanent } changes. In vivo, BR is supposed to absorb light, change conformation } to pump a proton across the membrane, then return to its initial } state, but I don't know whether this is true in your preparation. } I do not think that SEM will be useful, since you will not likely be } able to coat your specimen, and the thin, low Z specimen will produce } very little detectable signal, especially compared to the substrate. } AFM and STM can be used in an aqueous environment, and it is likely } that the membranes can be placed on a suitable substrate and imaged. } I do not know what the resolution limits of these techniques are, and } they only image surfaces, so if the changes do not alter the } topography, they will not be observed (unless there is some change in } the stiffness of the membrane, which could be detected in some AFM } modes). Any real experts in these techniques will be able to give you } much more authoritative information. Good luck. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 4, 22 -- From tivol-at-caltech.edu Tue Oct 7 18:42:14 2008 } 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) } 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m97NgD9o024230 } 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 7 Oct 2008 18:42:14 -0500 } 4, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) } 4, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 768CE66E0919; } 4, 22 -- Tue, 7 Oct 2008 16:42:13 -0700 (PDT) } 4, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new } 4, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) } 4, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 1408466E0AA6; } 4, 22 -- Tue, 7 Oct 2008 16:42:11 -0700 (PDT) } 4, 22 -- Message-Id: {BD298588-FF47-4697-A971-C94C14C4A581-at-caltech.edu} } 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 4, 22 -- To: Scott.Streiker-at-udri.udayton.edu, microscopy-at-microscopy.com } 4, 22 -- In-Reply-To: {200810070012.m970CNSG016237-at-ns.microscopy.com} } 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 4, 22 -- Content-Transfer-Encoding: 7bit } 4, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) } 4, 22 -- Subject: Re: [Microscopy] viaWWW: what kind of resolution can be obtained with various } 4, 22 -- Date: Tue, 7 Oct 2008 16:42:11 -0700 } 4, 22 -- References: {200810070012.m970CNSG016237-at-ns.microscopy.com} } 4, 22 -- X-Mailer: Apple Mail (2.929.2) } ==============================End of - Headers============================== }
-- Stephan H Coetzee Chief Technician Electron Microscope Unit University of Botswana
==============================Original Headers============================== 5, 38 -- From s.h.coetzee-at-gmail.com Wed Oct 8 01:06:44 2008 5, 38 -- Received: from fg-out-1718.google.com (fg-out-1718.google.com [72.14.220.153]) 5, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9866hmw016654 5, 38 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 01:06:43 -0500 5, 38 -- Received: by fg-out-1718.google.com with SMTP id 13so3134986fge.4 5, 38 -- for {microscopy-at-microscopy.com} ; Tue, 07 Oct 2008 23:06:42 -0700 (PDT) 5, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 38 -- d=gmail.com; s=gamma; 5, 38 -- h=domainkey-signature:received:received:message-id:date:from:to 5, 38 -- :subject:cc:in-reply-to:mime-version:content-type 5, 38 -- :content-transfer-encoding:content-disposition:references; 5, 38 -- bh=KXsUtIctGfW/Q9ZCIzkgbJA3YuL2Qo0qKfDcFzmGoYo=; 5, 38 -- b=sDsrI2zfgbrnQD9Ag8s1bYJ72MCXmj3ReBb/vk/dT3cmLeuPaSkQ/vQkXl45pPpf1I 5, 38 -- BrAa8ykAkqUgZLq/EattVJhkSWRnJTDNYdHIxv0KzfZfd8bwVjwaGxsJYw+jmJLpY9NB 5, 38 -- I+EgHgxaCOMwCBP/+4CJ+Ds9UKerrBEdjCNqM= 5, 38 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 38 -- d=gmail.com; s=gamma; 5, 38 -- h=message-id:date:from:to:subject:cc:in-reply-to:mime-version 5, 38 -- :content-type:content-transfer-encoding:content-disposition 5, 38 -- :references; 5, 38 -- b=lP0WAYkrUo+eNGfKYTp8J0o+rRvS3A5EUhU1mn3tEP+9mpdYa50a/gx+/4oU3qJbyf 5, 38 -- 876PQ2kQNSPXXkdvkyLVttdepLH/mDBLTsaeMoZbQZwsDpHJW1CQf3ruY3rfriwPxWp9 5, 38 -- q9WHeETLMkpHpth5zw57XtvLId2RoPme2IdBc= 5, 38 -- Received: by 10.86.65.9 with SMTP id n9mr7095598fga.55.1223446002902; 5, 38 -- Tue, 07 Oct 2008 23:06:42 -0700 (PDT) 5, 38 -- Received: by 10.86.80.13 with HTTP; Tue, 7 Oct 2008 23:06:42 -0700 (PDT) 5, 38 -- Message-ID: {1e3207cf0810072306h2fbbb3beyf7fe36af205cf30a-at-mail.gmail.com} 5, 38 -- Date: Wed, 8 Oct 2008 08:06:42 +0200 5, 38 -- From: "Stephan Coetzee" {s.h.coetzee-at-gmail.com} 5, 38 -- To: tivol-at-caltech.edu 5, 38 -- Subject: Re: [Microscopy] Re: viaWWW: what kind of resolution can be obtained with various 5, 38 -- Cc: microscopy-at-microscopy.com 5, 38 -- In-Reply-To: {200810072348.m97NmkID003072-at-ns.microscopy.com} 5, 38 -- MIME-Version: 1.0 5, 38 -- Content-Type: text/plain; charset=ISO-8859-1 5, 38 -- Content-Transfer-Encoding: 7bit 5, 38 -- Content-Disposition: inline 5, 38 -- References: {200810072348.m97NmkID003072-at-ns.microscopy.com} ==============================End of - Headers==============================
Please let me apologize for not responding more rapidly to the suggestion by Rosemary that we mix household bleach (5-6% Sodium Hypochlorite) with concentrated Hydrochloric Acid. Been a bit busy here (it was late Monday, CDT) and a long time since basic chemistry (we'll not say how many years). While it has been long since basic chemistry, as a virologist (my non EM side of life) who deals with disinfectants regularly, this mixture was intuitively not good (to understate the non chemical reaction).
The problem with bleach and HCl is the products of the chemical reaction. First, in the aqueous state, as bleach, the sodium hypochlorite reacts with the water to give hypochlorous acid. (For the sake of the chemists it looks like this)
NaOCl + H_2 O ↔ HOCl + Na^+ + OH^- .
Hypochlorous acid is the mechanism of both bleaching and disinfection. you want to kill a virus or other living organism, and do not care about structural impact - douse it with bleach. Why does Tony Soprano walk in with a case of bleach when he cuts up a body - destroys all the DNA - chops it up. HOCl does all sorts of nasty things.
Used appropriately bleach is fine. I also by it by the case. The real problem occurs when you mix in an acid, as with the HCl in this case. For the chemists, the reaction looks like:
HOCl + HCl ↔ H_2 O + Cl_2 ↑
That yellowish vapour that comes off is the Cl_2 ↑, or in the vernacular, chlorine gas.
In my humble opinion, this is not a good idea in the lab. If you really have to try it, at least use a chemical hood (not a BSL containment hood, below BSL3 they exhaust directly back to the room). A desiccator would not be considered acceptable containment.
Paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Oct 8 09:54:08 2008 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98Es7IZ002629 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 09:54:08 -0500 11, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 11, 20 -- (authenticated bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m98Erwvn021868; 11, 20 -- Wed, 8 Oct 2008 09:53:58 -0500 (CDT) 11, 20 -- Message-ID: {48ECC97F.90507-at-umanitoba.ca} 11, 20 -- Date: Wed, 08 Oct 2008 09:53:51 -0500 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Aclar film 11, 20 -- References: {200810062306.m96N66vl004059-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {200810062306.m96N66vl004059-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=UTF-8; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
Oh that did a real good job of chemical reactions. no superscripts, no subscripts, etc. the reations are:
1. NaOCl + H2O {-} HOCl + Na+ + OH-.
2. HOCl + HCl {-} H2O + Cl2 (gas)
let's see if this works, OK?
Paul
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 8, 20 -- From paul_hazelton-at-umanitoba.ca Wed Oct 8 10:04:42 2008 8, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98F4gaB015282 8, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 10:04:42 -0500 8, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 8, 20 -- (authenticated bits=0) 8, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m98F4YWp000724; 8, 20 -- Wed, 8 Oct 2008 10:04:34 -0500 (CDT) 8, 20 -- Message-ID: {48ECCBFB.9060507-at-umanitoba.ca} 8, 20 -- Date: Wed, 08 Oct 2008 10:04:27 -0500 8, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 8, 20 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 8, 20 -- MIME-Version: 1.0 8, 20 -- To: paul_hazelton-at-umanitoba.ca 8, 20 -- Subject: Re: [Microscopy] Re: Aclar film - chemical reactions 8, 20 -- References: {200810081458.m98Ew2Qr005890-at-ns.microscopy.com} 8, 20 -- In-Reply-To: {200810081458.m98Ew2Qr005890-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
Vendors have recommended battery back up systems for our new variable pressure SEM as well as our 4-year-old FEG SEM.
We do not experience the so-called *Florida Flicker* (i.e. power outages on a daily basis), however the power does go out unexpectedly from time to time.
What are the opinions concerning the benefits of having battery back up systems in place?
This information is being requested by the administration to justify the costs of the systems.
Any Horror stories from running scopes without the battery backup systems?
Thanks! Amanda
==============================Original Headers============================== 7, 20 -- From ALawrence-at-entomology.msstate.edu Wed Oct 8 12:56:02 2008 7, 20 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98Hu1M5008187 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:56:02 -0500 7, 20 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 7, 20 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id m98Hu0UR031859 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:56:01 -0500 7, 20 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 7, 20 -- with Novell_GroupWise; Wed, 08 Oct 2008 12:56:00 -0500 7, 20 -- Message-Id: {48ECADDC.B26A.00E6.0-at-entomology.msstate.edu} 7, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 7, 20 -- Date: Wed, 08 Oct 2008 12:55:57 -0500 7, 20 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 7, 20 -- To: {microscopy-at-microscopy.com} 7, 20 -- Subject: Battery back-up systems 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=ISO-8859-15 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Content-Disposition: inline ==============================End of - Headers==============================
I'm in the process of doing some freeze substitution and low temperature infiltration/embedding with HM20 in our Leica AFS system. I made up the HM20 according to the instructions included with the resin, mixed it very gently for a few minutes so as not to introduce any excess oxygen, and then placed the resin in a vacuum chamber at 25mm Hg for 10 or 15 min. to degas. After removal from the vacuum chamber, I put the resin in a capped 20mL scintillation vial and placed it in the AFS overnight to cool down to -50C. Now today when I open up the vial to pull out some resin to start infiltration, I noticed it has some small clumps of white precipitate floating around in it. Is this normal?
I pipetted some of the resin into another scintillation vial, capped it, and pulled it out of the AFS; as soon as the resin warmed up, the precipitate disappeared.
Is this a sign of water or some other unwanted substance in my resin, or am I just doing something horribly wrong?
Thank you, -- Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 8, 26 -- From bnross-at-interchange.ubc.ca Wed Oct 8 13:17:37 2008 8, 26 -- Received: from mr2.mail-relay.ubc.ca (mr2.mail-relay.ubc.ca [137.82.45.3]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98IHbs7022162 8, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 13:17:37 -0500 8, 26 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 8, 26 -- by mr2.mail-relay.ubc.ca (Postfix) with ESMTP id AC2FA17A2B 8, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 11:17:35 -0700 (PDT) 8, 26 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12]) 8, 26 -- by smtp.interchange.ubc.ca 8, 26 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 8, 26 -- with ESMTP id {0K8F0042AMTAHN-at-smtp.interchange.ubc.ca} for 8, 26 -- Microscopy-at-microscopy.com; Wed, 08 Oct 2008 11:17:35 -0700 (PDT) 8, 26 -- Date: Wed, 08 Oct 2008 11:17:34 -0700 (PDT) 8, 26 -- From: Bradford Ross {bnross-at-interchange.ubc.ca} 8, 26 -- Subject: White precipitate in HM 20 at low temps. 8, 26 -- To: Microscopy-at-microscopy.com 8, 26 -- Message-id: {25776586.5011223489854429.JavaMail.myubc2-at-brahms.my.ubc.ca} 8, 26 -- MIME-version: 1.0 8, 26 -- X-Mailer: uPortal WEB email client 3.0 8, 26 -- Content-type: text/plain; charset=us-ascii 8, 26 -- Content-transfer-encoding: 7bit 8, 26 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.10.8.180426 8, 26 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 8, 26 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_1200_1299 0, BODY_SIZE_5000_LESS 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0 8, 26 -- X-Spam-Level: 8, 26 -- X-Spam-Flag: No ==============================End of - Headers==============================
Dear List, Does anyone know offhand what the size of the unit cell of beef liver catalase is? Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Wed Oct 8 13:28:21 2008 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98ISK9c003235 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 13:28:21 -0500 2, 20 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by earth-doxen-postvirus (Postfix) with ESMTP id 8714366E0CC4 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 11:28:20 -0700 (PDT) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 2, 20 -- by earth-doxen-ssl (Postfix) with ESMTP id 63A2166E0C0E 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 11:28:19 -0700 (PDT) 2, 20 -- Message-Id: {AA9B4547-46FA-41F8-8521-F30C0DDCE121-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 2, 20 -- Subject: Catalase unit cell size 2, 20 -- Date: Wed, 8 Oct 2008 11:28:18 -0700 2, 20 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Interesting question, since there are discrepant answers in the literature. You might check out the following online reference:
www.pnas.org/content/78/8/4767.full.pdf
} Dear List, } Does anyone know offhand what the size of the unit cell of beef liver } catalase is? } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Dear Amanda, We have had infrequent unscheduled power shutdowns here at UBC, but all my electron microscopes have successfully protected themselves. The worst I have seen is the oil from the rotary pump going up the foreline on one SEM, once, because there wasn't an automatic vent valve on the foreline. It was fine once I cleaned out the oil, it didn't go any farther. The other SEMs and TEM have the automatic vent on the foreline and were fine. In fact, when the power came on, they started themselves up and pumped themselves back down to high vacuum. If it wasn't for the computers re-booting and the clocks being wrong, I would never know. Check with the manufacturers specifications of your instruments to see how they are protected against power and water flow interuptions. Most are quite good. Regards,
-----Original Message----- X-from: ALawrence-at-entomology.msstate.edu [mailto:ALawrence-at-entomology.msstate.edu] Sent: October 8, 2008 11:04 AM To: maryflet-at-interchange.ubc.ca
Greetings,
Vendors have recommended battery back up systems for our new variable pressure SEM as well as our 4-year-old FEG SEM.
We do not experience the so-called *Florida Flicker* (i.e. power outages on a daily basis), however the power does go out unexpectedly from time to time.
What are the opinions concerning the benefits of having battery back up systems in place?
This information is being requested by the administration to justify the costs of the systems.
Any Horror stories from running scopes without the battery backup systems?
Thanks! Amanda
==============================Original Headers============================== 7, 20 -- From ALawrence-at-entomology.msstate.edu Wed Oct 8 12:56:02 2008 7, 20 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98Hu1M5008187 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:56:02 -0500 7, 20 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 7, 20 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id m98Hu0UR031859 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:56:01 -0500 7, 20 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 7, 20 -- with Novell_GroupWise; Wed, 08 Oct 2008 12:56:00 -0500 7, 20 -- Message-Id: {48ECADDC.B26A.00E6.0-at-entomology.msstate.edu} 7, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 7, 20 -- Date: Wed, 08 Oct 2008 12:55:57 -0500 7, 20 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 7, 20 -- To: {microscopy-at-microscopy.com} 7, 20 -- Subject: Battery back-up systems 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=ISO-8859-15 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 17, 32 -- From maryflet-at-interchange.ubc.ca Wed Oct 8 14:03:28 2008 17, 32 -- Received: from mr8.mail-relay.ubc.ca (mr8.mail-relay.ubc.ca [137.82.45.15]) 17, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98J3RV9031516 17, 32 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 14:03:28 -0500 17, 32 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 17, 32 -- by mr8.mail-relay.ubc.ca (Postfix) with ESMTP id 2DFA51F51D 17, 32 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:03:24 -0700 (PDT) 17, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 17, 32 -- by smtp.interchange.ubc.ca 17, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 17, 32 -- with ESMTP id {0K8F00GH4OXNUK-at-smtp.interchange.ubc.ca} for 17, 32 -- microscopy-at-microscopy.com; Wed, 08 Oct 2008 12:03:24 -0700 (PDT) 17, 32 -- Date: Wed, 08 Oct 2008 12:03:20 -0700 17, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 17, 32 -- Subject: RE: [Microscopy] Battery back-up systems 17, 32 -- In-reply-to: {200810081804.m98I4EKg017960-at-ns.microscopy.com} 17, 32 -- To: ALawrence-at-entomology.msstate.edu 17, 32 -- Cc: microscopy-at-microscopy.com 17, 32 -- Reply-to: maryflet-at-interchange.ubc.ca 17, 32 -- Message-id: {0K8F00GH5OXNUK-at-smtp.interchange.ubc.ca} 17, 32 -- Organization: Materials Eng. 17, 32 -- MIME-version: 1.0 17, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 17, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 32 -- Content-type: text/plain; charset=us-ascii 17, 32 -- Content-transfer-encoding: 7bit 17, 32 -- Thread-index: AckpcEbwFWpYhil5QjuR83rCnE06GgABy8Zg 17, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.10.8.184614 17, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 17, 32 -- X-PerlMx-Spam: Probability=8%, Report=BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __USER_AGENT_MS_GENERIC 0 17, 32 -- X-Spam-Level: 17, 32 -- X-Spam-Flag: No ==============================End of - Headers==============================
I requested a UPS when we purchased our 300 kV TEM six years ago, mainly because we so often have one or a short series of power interruptions that last only a few seconds each. I've been saved from quite a bit of down time by riding out these quick power interruptions without the scope shutting down, since bringing the HT back up can take some time. The value of a UPS will depend to some extent on what equipment you have, how quick it is to recover from a power interruption, and whether the outages in your area tend to be of short or long duration.
Power interruptions may also be accompanied by spikes or sags in the incoming voltage, and these events can be damaging to sensitive electronics. We've had several instances of that here over the years. A UPS may protect the scope from some of these fluctuations.
Hope this helps.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. ********************************************************************* -----Original Message----- X-from: ALawrence-at-entomology.msstate.edu [mailto:ALawrence-at-entomology.msstate.edu] Sent: Wednesday, October 08, 2008 1:06 PM To: Elaine F. Schumacher
Greetings,
Vendors have recommended battery back up systems for our new variable pressure SEM as well as our 4-year-old FEG SEM.
We do not experience the so-called *Florida Flicker* (i.e. power outages on a daily basis), however the power does go out unexpectedly from time to time.
What are the opinions concerning the benefits of having battery back up systems in place?
This information is being requested by the administration to justify the costs of the systems.
Any Horror stories from running scopes without the battery backup systems?
Thanks! Amanda
==============================Original Headers============================== 7, 20 -- From ALawrence-at-entomology.msstate.edu Wed Oct 8 12:56:02 2008 7, 20 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98Hu1M5008187 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:56:02 -0500 7, 20 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 7, 20 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id m98Hu0UR031859 7, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 12:56:01 -0500 7, 20 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 7, 20 -- with Novell_GroupWise; Wed, 08 Oct 2008 12:56:00 -0500 7, 20 -- Message-Id: {48ECADDC.B26A.00E6.0-at-entomology.msstate.edu} 7, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 7, 20 -- Date: Wed, 08 Oct 2008 12:55:57 -0500 7, 20 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 7, 20 -- To: {microscopy-at-microscopy.com} 7, 20 -- Subject: Battery back-up systems 7, 20 -- Mime-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=ISO-8859-15 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 23, 24 -- From eschumacher-at-mccrone.com Wed Oct 8 15:10:52 2008 23, 24 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98KApDN017363 23, 24 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 15:10:52 -0500 23, 24 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 23, 24 -- Wed, 8 Oct 2008 15:10:51 -0500 23, 24 -- Content-class: urn:content-classes:message 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Type: text/plain; 23, 24 -- charset="US-ASCII" 23, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 24 -- Subject: RE: [Microscopy] Battery back-up systems 23, 24 -- Date: Wed, 8 Oct 2008 15:10:41 -0500 23, 24 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150E16D-at-MCCRONEMSG.tmg.mccrone.com} 23, 24 -- In-Reply-To: {200810081806.m98I64Gm021167-at-ns.microscopy.com} 23, 24 -- X-MS-Has-Attach: 23, 24 -- X-MS-TNEF-Correlator: 23, 24 -- Thread-Topic: [Microscopy] Battery back-up systems 23, 24 -- Thread-Index: AckpcIaV5PnNMhLpRBqlhIVOoZB3qQADbvcA 23, 24 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 23, 24 -- To: {microscopy-at-microscopy.com} 23, 24 -- X-OriginalArrivalTime: 08 Oct 2008 20:10:51.0375 (UTC) FILETIME=[F6228FF0:01C92981] 23, 24 -- Content-Transfer-Encoding: 8bit 23, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m98KApDN017363 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nabil.bassim-at-nrl.navy.mil as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Introductory text for TEM of soft materials
Question: Hi,
I'm interested if anyone has a suggestion for a good introductory text for performing TEM on soft materials, such as peptides? I am a microscopist with training in hard materials and want a good reference with regards to sample preparation, imaging conditions, proper voltages, the need for cryo, etc.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both grace.jang-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: grace.jang-at-asu.edu Name: Grace
Organization: arizona state university
Title-Subject: [Filtered] element mapping on E.coli by EDX
Question: I am trying to use the EDX for elemental mapping on E.coli.
1. Would you please tell me how E.coli samples have to be prepared to use the SEM/EDX?
2. I want to know where chlorine elements are located on E.coli samples after chlorination. Is the EDX analysis an appropriate approach to know the elemental mapping?
3. Except EDX, is there any way I can observe the element location on microbiological samples?
Yes, we do this in the fume hood, of course! I guess I should have said this....
cheers, Rosemary ________________________________________ X-from: paul_hazelton-at-umanitoba.ca [paul_hazelton-at-umanitoba.ca] Sent: Thursday, 9 October 2008 2:03 a.m. To: White, Rosemary (PI, Black Mountain)
Please let me apologize for not responding more rapidly to the suggestion by Rosemary that we mix household bleach (5-6% Sodium Hypochlorite) with concentrated Hydrochloric Acid. Been a bit busy here (it was late Monday, CDT) and a long time since basic chemistry (we'll not say how many years). While it has been long since basic chemistry, as a virologist (my non EM side of life) who deals with disinfectants regularly, this mixture was intuitively not good (to understate the non chemical reaction).
The problem with bleach and HCl is the products of the chemical reaction. First, in the aqueous state, as bleach, the sodium hypochlorite reacts with the water to give hypochlorous acid. (For the sake of the chemists it looks like this)
NaOCl + H_2 O ¡ê HOCl + Na^+ + OH^- .
Hypochlorous acid is the mechanism of both bleaching and disinfection. you want to kill a virus or other living organism, and do not care about structural impact - douse it with bleach. Why does Tony Soprano walk in with a case of bleach when he cuts up a body - destroys all the DNA - chops it up. HOCl does all sorts of nasty things.
Used appropriately bleach is fine. I also by it by the case. The real problem occurs when you mix in an acid, as with the HCl in this case. For the chemists, the reaction looks like:
HOCl + HCl ¡ê H_2 O + Cl_2 ¡è
That yellowish vapour that comes off is the Cl_2 ¡è, or in the vernacular, chlorine gas.
In my humble opinion, this is not a good idea in the lab. If you really have to try it, at least use a chemical hood (not a BSL containment hood, below BSL3 they exhaust directly back to the room). A desiccator would not be considered acceptable containment.
Paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Oct 8 09:54:08 2008 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m98Es7IZ002629 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 8 Oct 2008 09:54:08 -0500 11, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 11, 20 -- (authenticated bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m98Erwvn021868; 11, 20 -- Wed, 8 Oct 2008 09:53:58 -0500 (CDT) 11, 20 -- Message-ID: {48ECC97F.90507-at-umanitoba.ca} 11, 20 -- Date: Wed, 08 Oct 2008 09:53:51 -0500 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Aclar film 11, 20 -- References: {200810062306.m96N66vl004059-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {200810062306.m96N66vl004059-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=UTF-8; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
The University of Memphis Director, Integrated Microscopy Center
Applications are invited for the Director of the Integrated Microscopy Center (IMC) at the University of Memphis. This is a tenure-track Assistant/Associate Professor position in the Department of Biology. Applicants must have a Ph.D. or equivalent degree in cell/molecular biology, and a solid record of peer-reviewed publications. Preference will be given to those applicants with a history of an externally-funded research program and mentoring of M.S./Ph.D. students. We seek candidates with broad expertise in microscopy, and a commitment to managing a multi-user laboratory. The anticipated start date is August 2009.
The University of Memphis is a comprehensive state university with an enrollment of approximately 21,000 students. The IMC is a fee for service University wide facility that is also used by the surrounding biomedical community. The IMC has confocal, light, atomic force and electron microscopes with ancillary equipment and is staffed by skilled technicians. The Director will be responsible for a budget of around $250,000 per year and will supervise a staff of 3-5 people. The IMC had about 60 users last academic year. To offset the administrative component of this position, the teaching load of the Director will be reduced by half. A complete description can be found on our web site given below. The Department of Biology offers B.S., M.S., and Ph.D. degrees in Biology. There are approximately 30 faculty, 14 staff, 50 full-time graduate students, and over 700 majors in the department. The department administers the Meeman Biological Field Station and the Ecological Research Center, and is closely affiliated with the interdisciplinary Bioinformatics Program, and the W. Harry Feinstone Center for Genomic Research.
Additional information: IMC information http://www.memphis.edu/microscopy Departmental information http://biology.memphis.edu University information http://www.memphis.edu, or contact Diane Mittelmeier (search coordinator) (901) 678-4469 (dmittlmr-at-memphis.edu). Applicants should submit a letter of application, curriculum vitae, a concise description of research and teaching interests and expertise in microscopy. Please send the names, phone numbers and email addresses of at least four references (do not send reference letters) to: http://workforum.memphis.edu. Closing date for application is November 17, 2008. Women and minority candidates are encouraged to apply. The University of Memphis is an Affirmative Action/Equal Opportunity Employer. IMC Director Search:
Lewis Coons Ph D, Director IMC Chair Search Committee
==============================Original Headers============================== 7, 26 -- From lcoons-at-memphis.edu Thu Oct 9 15:00:46 2008 7, 26 -- Received: from itmta2.memphis.edu (itmx2.memphis.edu [141.225.112.71]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m99K0jpr003831 7, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 9 Oct 2008 15:00:46 -0500 7, 26 -- Received: from itexfe7.uom.memphis.edu (itexfe7.memphis.edu [10.15.1.68]) 7, 26 -- by itmta2.memphis.edu (Postfix) with ESMTP id B71E4A0C0E0 7, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 9 Oct 2008 15:00:45 -0500 (CDT) 7, 26 -- Received: from itexbe7.uom.memphis.edu ([10.15.1.66]) by 7, 26 -- itexfe7.uom.memphis.edu ([10.15.1.68]) with mapi; Thu, 9 Oct 2008 15:00:45 7, 26 -- -0500 7, 26 -- From: "Lewis B Coons (lcoons)" {lcoons-at-memphis.edu} 7, 26 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 7, 26 -- Date: Thu, 9 Oct 2008 15:00:43 -0500 7, 26 -- Subject: Position of Director, University Imaging Center 7, 26 -- Thread-Topic: Position of Director, University Imaging Center 7, 26 -- Thread-Index: AckqSbXtJug4NKUR+kqUbyFXoq0cNA== 7, 26 -- Message-ID: {C513CD1B.820A%lcoons-at-memphis.edu} 7, 26 -- Accept-Language: en-US 7, 26 -- Content-Language: en 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- acceptlanguage: en-US 7, 26 -- Content-Type: text/plain; charset="iso-8859-1" 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m99K0jpr003831 ==============================End of - Headers==============================
Post doctoral position in Material Characterization by TEM at the Aristotle University of Thessaloniki, Greece for 20 months starting from May 1st, 2009
The successful candidate will work on the microstructural characterization using conventional and high resolution Transmission Electron Microscopy (TEM). Previous experience on materials characterization by TEM is mandatory. A strong involvement and participation in MANSiC network activities is envisaged.
Benefits - Training on SiC structural characterization by TEM, by highly experienced scientists. - Complementary skills to be acquired during the position: web technology, language courses, management... - International and industrial contacts within MANSiC network (11 world leadership laboratories). - Participation in International meetings (conferences, workshops, training schools). - The fellowship is well paid, including a basic living and a mobility allowance (with deductions by national laws) and travel and career exploratory allowances according to the Marie Curie rules. A generous career development package is included.
Requirements - Ph.D. level in the study of materials by TEM. - 4 to 10 years of research experience since obtaining of the master degree or equivalent diploma giving access to doctoral studies. - Good experience on the use and maintenance of TEM. - Theoretical and practical knowledge of structural characterization techniques. - Good English level and computing skills are mandatory. - Nationality and transnational mobility conditions according to RTN rules
Job Starting Date: May 1st 2009 Application Deadline: January 31rst 2009
More information can be found at http://www.mansic.eu/index.htm
E.K. Polychroniadis Department of Physics Aristotle University of Thessaloniki Thessaloniki 54124, Greece Tel.: +30.2310.998163 Fax: +30.2310.998241 e-mail: polychr-at-auth.gr
==============================Original Headers============================== 16, 23 -- From polychr-at-auth.gr Fri Oct 10 09:48:30 2008 16, 23 -- Received: from hyperion.ccf.auth.gr (hyperion.ccf.auth.gr [155.207.1.42]) 16, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9AEmTMr003419 16, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 09:48:29 -0500 16, 23 -- Received: from kerasia (kerasia.physics.auth.gr [155.207.11.93]) 16, 23 -- by hyperion.ccf.auth.gr (8.14.3/8.14.3) with ESMTP id m9AEmRRb007777 16, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 17:48:28 +0300 16, 23 -- Reply-To: {polychr-at-auth.gr} 16, 23 -- From: "Polychroniadis Stathis" {polychr-at-auth.gr} 16, 23 -- To: {Microscopy-at-microscopy.com} 16, 23 -- Subject: TEM Post-doc position 16, 23 -- Date: Fri, 10 Oct 2008 17:48:28 +0300 16, 23 -- Organization: a.u.th. 16, 23 -- Message-ID: {9BAFAB2CE7464D0199BB288A2248225C-at-physics.auth.gr} 16, 23 -- MIME-Version: 1.0 16, 23 -- Content-Type: text/plain; 16, 23 -- charset="us-ascii" 16, 23 -- Content-Transfer-Encoding: 7bit 16, 23 -- X-Mailer: Microsoft Office Outlook 11 16, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 16, 23 -- Thread-Index: Ackq50Gob8uopfQXSFWdSunSmtpIQQ== 16, 23 -- X-Virus-Scanned: ClamAV version 0.94, clamav-milter version 0.94 on artemis.ccf.auth.gr 16, 23 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Both Delta College and MATC Madison do a fantastic job training EM technicians.
Unfortunately many Universities have a minimum requirement of a Batchelors level degree for these sort of positions.
Alan
At 11:23 AM 10/6/2008, jkrupp-at-deltacollege.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Alan W Nicholls, PhD Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Several points and I hope this isn't a bitter rant but observations: Randy's original post was about "advanced techs" My interpretation of that is: experience. Performing a protocol once does not compare with doing it dozens or hundreds of times since the multitude of variables we encounter often change and present problems. I often encounter people who can follow a technique or operate an instrument according to instructions but are totally lost when something doesn't go correctly. The ability to troubleshoot is often aided by understanding the principal theories but also relates to maturity and knowledge of resources. A good experienced technologist can take a scientific question and develop or find defensible protocols and return with data to answer the question. The lack of highly qualified advanced techs is certainly related to to pay scale but perhaps also to less mobility. Many positions that an experienced technologist could fill are now filled with PhDs. That degree often allows for a higher salary. This degree ceiling was not apparent 25 years ago. Talented people will not choose a path with such a low ceiling. I am mostly familiar with the academic world so industry may present more options. Academia often has a disposable tech disposition. Hire young people for less then replace them when their salaries rise and the money gets tight. Experienced people are older by definition. I have less desire to move and leave friends now than when I was 30. The wonders of New York are attractive but cannot compensate for weekends in the Sierra mountains. Another thought: experienced people in a satisfactory position are not looking for another position. They must be searched out and recruited.
Larry
nicholls-at-uic.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jon } } Both Delta College and MATC Madison do a fantastic job training EM } technicians. } } Unfortunately many Universities have a minimum requirement of a Batchelors } level degree for these sort of positions. } } Alan } } At 11:23 AM 10/6/2008, jkrupp-at-deltacollege.edu wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } On Oct 6, 2008, at 9:02 AM, randy-nessler-at-uiowa.edu wrote: } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } It seems to me that there is a shortage of "advanced" biological EM } } } techs out there. By advanced, I mean somebody that can come in and } } } champion a project, maybe perform some Immunolabeling. } } } } } } I know of three, possibly four labs looking and they are not getting } } } very many applicants. I know one lab ran an ad through MSA and got two } } } applicants. Is it a salary issue or is it a shortage of skilled } } } workers? } } } Combination of both? } } } } } } What do labs do when they don't have the time to "groom" an employee? } } OK, I'll bite. I don't know what the issues might be, but it's my job } } now to try and produce the kind of tech you are asking about. } } } } What sort of things make an attractive EM tech these days? We have a } } fairly comprehensive curriculum, but it could always be improved. } } } } What are the top skills that are needed by labs? How much do you want } } new employees to know vs what you would rather train them for on your } } terms? } } } } How much photographic skill, Photoshop only or other things, how much } } vacuum experience, how about instrument operation and theory. } } } } Do you want people who just know how to drive the bus or do you want } } them to know something about science as well? } } } } I don't get out as much as I used too, I am counting on you all to } } help me know what it is like out there in real labs and enterprises. } } } } Let me know what you value in a trained technician and I will try to } } include those ideas in our training program. } } } } Jon } } } } } } Jonathan Krupp } } Delta College } } 5151Pacific Ave. } } Stockton, CA 95207 } } 209-954-5284 } } jkrupp-at-deltacollege.edu } } } } } } } } } } ==============================Original Headers============================== } } 16, 38 -- From jkrupp-at-deltacollege.edu Mon Oct 6 11:22:30 2008 } } 16, 38 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu } } [207.62.178.236]) } } 16, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id m96GMT7r025786 } } 16, 38 -- for {Microscopy-at-microscopy.com} ; 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} } 16, 38 -- Mon, 6 Oct 2008 09:22:28 -0700 (PDT) } } 16, 38 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) } } 16, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 1C0628F74DC3 } } 16, 38 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Oct 2008 09:22:28 } } -0700 (PDT) } } 16, 38 -- Message-Id: {E8BE9901-CBA6-478D-AFBF-016E55A47829-at-deltacollege.edu} } } 16, 38 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } } 16, 38 -- To: Microscopy-at-microscopy.com } } 16, 38 -- In-Reply-To: {200810061602.m96G280f024158-at-ns.microscopy.com} } } 16, 38 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } } 16, 38 -- Content-Transfer-Encoding: 7bit } } 16, 38 -- Mime-Version: 1.0 (Apple Message framework v929.2) } } 16, 38 -- Subject: Re: [Microscopy] Biological EM Techs } } 16, 38 -- Date: Mon, 6 Oct 2008 09:22:27 -0700 } } 16, 38 -- References: {200810061602.m96G280f024158-at-ns.microscopy.com} } } 16, 38 -- X-Mailer: Apple Mail (2.929.2) } } ==============================End of - Headers============================== } } Alan W Nicholls, PhD } Director of Research Service Facility (Electron Microscopy) } Research Resources Center - East (M/C 337) } Room 110 Science and Engineering South Building } The University of Illinois at Chicago } 845 West Taylor St } Chicago, IL 60607-7058 } } Tel: 312 996 1227 } Fax: 312 996 8091 } Office: Room 110 } } Web site www.rrc.uic.edu } } } ==============================Original Headers============================== } 12, 19 -- From nicholls-at-uic.edu Fri Oct 10 10:57:42 2008 } 12, 19 -- Received: from mail-4.priv.cc.uic.edu (mail-4.cc.uic.edu [128.248.155.184]) } 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9AFvgQo019382 } 12, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 10:57:42 -0500 } 12, 19 -- Received: from vmware.uic.edu (emf2.rrc.uic.edu [131.193.156.241]) } 12, 19 -- (authenticated bits=0) } 12, 19 -- by mail-4.priv.cc.uic.edu (8.14.0/8.14.0) with ESMTP id m9AFvfui009821 } 12, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 12, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 10:57:41 -0500 } 12, 19 -- Message-Id: {6.2.1.2.2.20081010104705.0363c0f8-at-mail.uic.edu} } 12, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.1.2 } 12, 19 -- Date: Fri, 10 Oct 2008 10:57:35 -0500 } 12, 19 -- To: Microscopy-at-microscopy.com } 12, 19 -- From: Alan Nicholls {nicholls-at-uic.edu} } 12, 19 -- Subject: Re: [Microscopy] Re: Biological EM Techs } 12, 19 -- In-Reply-To: {200810061623.m96GNE4T026986-at-ns.microscopy.com} } 12, 19 -- References: {200810061623.m96GNE4T026986-at-ns.microscopy.com} } 12, 19 -- Mime-Version: 1.0 } 12, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } ==============================End of - Headers============================== }
-- Larry Ackerman, Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-476-4400
==============================Original Headers============================== 8, 39 -- From Larry.Ackerman-at-ucsf.edu Fri Oct 10 18:14:24 2008 8, 39 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 8, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9ANENk0007311 8, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 18:14:24 -0500 8, 39 -- Received: from [64.54.35.210] by emfmcb01.ucsfmedicalcenter.org with 8, 39 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.3.2)); 8, 39 -- Fri, 10 Oct 2008 16:14:18 -0700 8, 39 -- X-Server-Uuid: 70AB4C1F-E30B-44E9-99F3-BC3762B66E5B 8, 39 -- X-AuditID: 403623d2-aaed0bb000000c9c-52-48effde318b5 8, 39 -- Received: from exbhmcb02.ucsfmedicalcenter.org ( 8, 39 -- exbhmcb02.ucsfmedicalcenter.org [64.54.46.223]) by 8, 39 -- vsobmcb02.ucsfmedicalcenter.org (Symantec Mail Security) with ESMTP id 8, 39 -- 6CB97143C for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 18:14:11 8, 39 -- -0700 (PDT) 8, 39 -- Received: from exvs06.net.ucsf.edu ([64.54.128.152]) by 8, 39 -- exbhmcb02.ucsfmedicalcenter.org with Microsoft SMTPSVC(6.0.3790.1830); 8, 39 -- Fri, 10 Oct 2008 16:14:18 -0700 8, 39 -- Received: from Ralston-Lab-Larry-Ackerman.local ([128.218.123.88]) by 8, 39 -- exvs06.net.ucsf.edu with Microsoft SMTPSVC(6.0.3790.3959); Fri, 10 Oct 8, 39 -- 2008 16:14:10 -0700 8, 39 -- Message-ID: {48EFE1C2.40101-at-ucsf.edu} 8, 39 -- Date: Fri, 10 Oct 2008 16:14:10 -0700 8, 39 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 8, 39 -- Reply-to: larry.ackerman-at-ucsf.edu 8, 39 -- Organization: UCSF, NeuroAnatomy 8, 39 -- User-Agent: Thunderbird 2.0.0.16 (Macintosh/20080707) 8, 39 -- MIME-Version: 1.0 8, 39 -- To: Microscopy-at-microscopy.com 8, 39 -- Subject: Re: [Microscopy] Biological EM Techs 8, 39 -- References: {200810101603.m9AG3RqL029806-at-ns.microscopy.com} 8, 39 -- In-Reply-To: {200810101603.m9AG3RqL029806-at-ns.microscopy.com} 8, 39 -- X-OriginalArrivalTime: 10 Oct 2008 23:14:10.0993 (UTC) 8, 39 -- FILETIME=[E73E9210:01C92B2D] 8, 39 -- X-Brightmail-Tracker: AAAAAQwm990= 8, 39 -- X-WSS-ID: 64F13E401OS1138364-01-01 8, 39 -- Content-Type: text/plain; 8, 39 -- charset=iso-8859-1; 8, 39 -- format=flowed 8, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dr. George Palade, a pioneer in cell biology and biological electron microscopy, has died at the age of 95. I certainly remember reading his papers and celebrating his Nobel Prize in Physiology and Medicine in 1974 as I started my career as an electron microscopist!
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 19 -- From tina-at-pbrc.hawaii.edu Fri Oct 10 19:59:50 2008 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9B0xoCQ023951 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 19:59:50 -0500 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id m9B0xl8q025164 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 14:59:47 -1000 (HST) 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id m9B0xkxI025161 5, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Oct 2008 14:59:46 -1000 (HST) 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 19 -- Date: Fri, 10 Oct 2008 14:59:45 -1000 (HST) 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 19 -- X-Sender: tina-at-halia 5, 19 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 19 -- Subject: George Palade, cell biologist 5, 19 -- Message-ID: {Pine.GSO.4.21.0810101452360.25142-100000-at-halia} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
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I look for the chemical composition Bohner's etchant, which produces excellent results when used to etch the grain boundaries of alloy EN AW 6060 (approx. 0.5% Si).
best regards
Chris Hübner Foundry Reseach Institute Kraków, Poland
==============================Original Headers============================== 6, 22 -- From hubner-at-iod.krakow.pl Mon Oct 13 02:33:11 2008 6, 22 -- Received: from sowa.iod.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9D7XAQE027007 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 13 Oct 2008 02:33:10 -0500 6, 22 -- Received: from iodkh007 (sroka.IOd.krakow.pl [149.156.29.200]) 6, 22 -- by sowa.iod.krakow.pl (8.13.6+Sun/8.13.6) with SMTP id m9D7DSqL023942 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 13 Oct 2008 09:13:28 +0200 (CEST) 6, 22 -- Message-ID: {3843AEEE21BA4853B3EBFC2530FAA14E-at-iodkh007} 6, 22 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-iod.krakow.pl} 6, 22 -- To: "Microscopy list" {microscopy-at-microscopy.com} 6, 22 -- Subject: chemical composition Bohner's etchant for AL alloys 6, 22 -- Date: Mon, 13 Oct 2008 09:33:08 +0200 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- format=flowed; 6, 22 -- charset="iso-8859-2"; 6, 22 -- reply-type=original 6, 22 -- Content-Transfer-Encoding: 8bit 6, 22 -- X-Priority: 3 6, 22 -- X-MSMail-Priority: Normal 6, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 6, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Not exactly on topic, but has anyone else requested permission to reproduce an image(s) from a paper in a review article, and been asked for payment before permission is granted? I don't remember ever having to do this in the past. thanks, Rosmeary WHite
Yes, we've been seeing that a lot. Sometimes, if your editor contacts the publisher, there is a professional courtesy extended and the cost may be waived.
Some publications are more "revenue oriented" than others. I guess it's a reflection of the scramble for the few remaining dollars left in the economy and an indicator of things to come.
JB
} Not exactly on topic, but has anyone else requested permission to } reproduce an image(s) from a paper in a review article, and been } asked for payment before permission is granted? I don't remember } ever having to do this in the past. } thanks, } Rosmeary WHite
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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Email: ecoagripolicy-at-gmail.com Name: Rachel
Organization: UWA
Title-Subject: [Filtered] re: lignin vs. suberin
Question: Dear group members, I have been trying to interpret my results comparatively. My autofluorescence results on observing rice roots, shows that one treatment has lignin (green) throughout. Whereas when I used berberine stain I find that lignin and suberin are localised. Can anyone give an explanation? Any suggestions to resolve this issue? How to localise lignin from suberin effectively? Regards, Rachel.
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Title-Subject: [Filtered] (wanted) Fischione Model 2020 advanced tomography holder
Question: Hi,
We are looking for a second-hand Fischione Model 2020 advanced tomography holder. Please contact me offline for futher details.
Thanks, Changbae
--------------------------------- Changbae Hyun Postdoc University of Arkansas Department of Physics 226 Physics Building 835 West Dickson Street Fayetteville, AR 72701 Phone (512) 554-5990 E-mail: changbaehyun-at-gmail.com
Mark; We used the User Function Control for several years. The lens currents are factory supplied, but reside in an unexpected place: The hex codes for lens conditions in the push-button probe control for 'EDS', 'NBD', and 'CBD' are ideal for a wide variety of STEM probe conditions. We had our JEOL master rep (Masa Kawasaki) program the UFC. Later, when we upgraded to FASTEM computer control (which is JEOL USA only) we used the same programs. The best part about this is that the STEM never turns on the mini lens and the thermal drift that plagues the normal STEM system is not there.
John Mardinly, Numonyx
-----Original Message----- X-from: mgb-at-ansto.gov.au [mailto:mgb-at-ansto.gov.au] Sent: Thursday, September 25, 2008 2:16 AM To: MARDINLY, A
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgb-at-ansto.gov.au as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: mgb-at-ansto.gov.au Name: Mark Blackford
Organization: ANSTO
Title-Subject: [Filtered] STEM-HAADF procedure
Question: Hi All,
does anyone have a procedure they would like to share for setting up lens configurations and calibrating collection angles for STEM-HAADF imaging with a JEOL 2010F (S)TEM?
Any advice or references would be appreciated. Cheers,
The Midwest Microscopy and Microanalysis Society will hold a meeting in conjunction with the Madison Area Technical College Electron Microscopy Program on Tuesday, October 28, 2008, at the MATC campus in Madison, Wisconsin. Program and registration information can be found on our website:
www.midwestmicroscopy.org
Please follow the link to the Meetings page.
Regards,
Elaine Schumacher M3S Program Chair
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 10, 23 -- From eschumacher-at-mccrone.com Wed Oct 15 07:37:33 2008 10, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9FCbX7M029030 10, 23 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2008 07:37:33 -0500 10, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Wed, 15 Oct 2008 07:37:33 -0500 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="iso-8859-1" 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society and Madison Area Technical College 10, 23 -- Date: Wed, 15 Oct 2008 07:36:55 -0500 10, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150E193-at-MCCRONEMSG.tmg.mccrone.com} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society and Madison Area Technical College 10, 23 -- Thread-Index: AckuwrSXt0V8B0DIRiuA/u9PyQ/2bg== 10, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 15 Oct 2008 12:37:33.0452 (UTC) FILETIME=[CBCD1CC0:01C92EC2] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9FCbX7M029030 ==============================End of - Headers==============================
we are seeking abstract submissions for the upcoming International Clay Conference, scheduled from 14-20 June, 2009 in Castellaneta, Italy (see http://www.14icc.org/). Our session (HE1) deals with monitoring, identification, and quantification of asbestos associated with human exposures. These studies are very important to both the regulatory community, because special precautions must be taken when asbestos is found in significant amounts to warrant government intervention, and industry, which is charged with providing us the natural resources required for modern life. Different techniques of monitoring and analysis are necessary depending on where the asbestos occurs (e.g., air, water, soil, rocks, biological materials, asbestos-containing materials, their transformation products, and even inside the human body). This session will: 1) present the state-of-the-art in monitoring techniques which are considered the most suitable for the different materials in which asbestos can occur; 2) compare various analytical methods; 3) exchange information about the advances in this topic; and 4) develop interdisciplinary collaborations.
Note the abstract deadline is 31 December, 2008.
The conveners of the session are:
Elena Belluso • {mailto:elena.belluso-at-unito.it} elena.belluso-at-unito.it
Those of you who are connected with IUCr may already have seen this, to you I apologize for the repetition.
For those who have not seen it, I thought you might like to see the following letter from Professor Rams, a professor of crystallography in Cuba, forwarded by the International Union of Crystallography.
Alwyn
Dear Mike,
As you may have known from the news, Cuba has been affected by two huge hurricanes in less than a week time. The worse hit regions in the country have been the most occidental province of Pinar del Rio, land of the world famous tobacco, and the Isle of Youth which is at the south of the mainland island. Also the East part of the country was severely hit Guantanamo, Holguin, Ciego de Avila, Las Tunas, Granma. Cuba has 14 provinces and at least 12 have been heavily affected by the hurricanes. Habana fortunately was not direct on the path of the hurricanes eye and although both were close to it, the damages in infrastructure was not as bad as other regions of the country.
Every news you have heard or seen pales compared to the size of the damage.
We, over the years, have gotten used to hurricanes but this time it has surpass anything we could imagine. It has been compared as worse than a massive air strike in terms of damage. Whole regions of the country have been devastated and hundred of thousands of houses have been damaged or totally destroyed. Today they were reporting that at least half a million houses have been affected. There are big regions without electricity. Roads and bridges have been damaged. Crops lost completely. Farm animals and cattle also lost by the thousands.
In the middle of such tremendous disaster there have been also incredible events of solidarity, discipline and courage. This has also been one of this moment were you can feel proud of your country and your countrymen. In spite of the ferocious nature of the events, up to now only seven people have lost there life. More than one million and a half people were evacuated with no human loss during this evacuation. It is estimated that more than seventy percent of those evacuated did so to the houses of other Cubans who open their doors to shelter those more in need and are sharing with others the little they have. In Cuba we say that solidarity is not to give what you have surplus, but to share the things that you also need. All pregnant women with risk were taken to secure places and put under medical surveillance.
Elderly people have been taken also under medical care. Not a single child has lost his life. Brigades of the finest Cuban artist have gone to the worse affected areas to bring their art and joy to everyone. They sleep in camps with the evacuated, they eat with them, and share the life with them. They have declare their commitment to stay as long as it takes.
Specially touching is the actions they are doing with the children in order to minimize the psychological impact of the disaster. Thousands and thousands of Cubans are working in emergency actions for those affected with no regard to day or night, weekdays or weekends, reconstruction efforts, saving as much of the agriculture possible. Nobody has been left unattended.
To witness people who have lost all there belongings including there houses saying on radio or TV that they will overcome tragedy and they are confident that they will not be forgotten by society. To see these people standing over the wreckage with such a strength and optimism makes all of us, who have not been so much affected, get the spirit to do what is needed to win this battle against adversity.
In the middle of the destruction school is starting today and the plans are to incorporate all our students from the basic school up to university in less than a week. There will be no single kid in Cuba without a teacher and a classroom. That is, for us teachers and professors, the biggest immediate challenge. But, we also have to care about middle and long term consequence of this disaster. We have to think how can we not only survive but also move forward. We have to make our universities stronger and better. Our middle term challenge is to be capable of giving to our young students a better education and to graduate more skillful professionals that can better serve their country.
In our institute we are assessing damages and discussing what to do. Our buildings did not suffer to much but some of our scientific equipments, already poor and outdated, will not survive this blow as they have been soaked in water and in our tropical climate corrosion will unavoidably render them useless in no time and the electronic parts are also heavily damaged. Part of the ceiling were blown away in one of our building and air conditioner and acclimatization equipment was partially damaged. Rain was our worse enemy.
We would like through the IUCr, to reach to all our colleagues friends in crystallography around the world to help us as much as they can. Any help in infrastructure, equipment or financial resources is welcomed. The goal is to rebuild our crystallography laboratory that is the only one in Cuba Universities. Today we do not know if we will be able to recover our old diffractometers and the country is pulling all their resources in much urgent needs that ours. Some may think that trying to make science in ours today condition makes no sense and we should instead concentrate in other efforts. We instead strongly believe that the capability of a country to rise itself depends in its ability to see beyond the urgent and project itself to the future. There is much more than buildings and houses to be reconstructed. To loose our ability to make science is to renounce to build a better country. To loose our ability to teach students in science, build skillful human resources, is to renounce to build a better country. We can not do that, and we will not do that. At least that much we owe to our youngest sons. Everything needed to make this possible will be done. We know we are not alone and we can count with our friends all over the world. If also ask if the IUCr could help us to channel any help that friends are willing to give us.
We have also decided to go on as planned with our crystallography school for July 2009. As you know we organize every two years an international school on crystallography were students mainly from Cuba and Latin America come and get first hand state of the art lectures by invited renowned professors. Over the years this school has been an important part in the curricula of our students in physics, material science, chemistry, engineering and life science. To the school attend students from all over the country and from all universities in the country. We will submit the proposal for support to the IUCr in the next days. We also ask for professors willing to come to our school covering their cost and give lectures to please contact me so we can arrange the school program. We would like this year to give emphasis in protein crystallography, from single crystal to recent developments in powder methods. Yet as usual the school is open to other subjects.
I take the opportunity to thank you personally for the friendly support over the years.
Kind regards
Ernesto
Dr. Ernesto Estevez Rams Deputy Director Instituto de Ciencia y Tecnologia de Materiales Universidad de la Habana San Lazaro y L. CP 10400 Cuba
estevez-at-imre.oc.uh.cu
tel: (+537) 870-7666
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 36, 21 -- From jae5-at-lehigh.edu Wed Oct 15 07:59:55 2008 36, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 36, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9FCxtS2024420 36, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Oct 2008 07:59:55 -0500 36, 21 -- Received: from [127.0.0.1] (Dyn055140.mat.Lehigh.EDU [128.180.55.140]) 36, 21 -- (authenticated bits=0) 36, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id m9FCxsIJ024739 36, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 36, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Oct 2008 08:59:54 -0400 36, 21 -- Message-ID: {48F5E950.60403-at-lehigh.edu} 36, 21 -- Date: Wed, 15 Oct 2008 09:00:00 -0400 36, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 36, 21 -- Organization: Lehigh University 36, 21 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 36, 21 -- MIME-Version: 1.0 36, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 36, 21 -- Subject: Cuba after the hurricane 36, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 36, 21 -- Content-Transfer-Encoding: 7bit 36, 21 -- X-Virus-Scanned: ClamAV version 0.94, clamav-milter version 0.94 on rain.cc.lehigh.edu 36, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
If you already visited our website for meeting information following my posting earlier today, please note that RSVPs for the October 28 meeting at MATC in Madison, WI should be sent to:
wcarmichael-at-matcmadison.edu
The PDF file posted on our website contains an error in the email address, and the situation is being corrected. I apologize for any inconvenience that this might have caused.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
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==============================Original Headers============================== 10, 23 -- From eschumacher-at-mccrone.com Wed Oct 15 12:18:05 2008 10, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9FHI571005098 10, 23 -- for {microscopy-at-microscopy.com} ; Wed, 15 Oct 2008 12:18:05 -0500 10, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Wed, 15 Oct 2008 12:18:05 -0500 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="iso-8859-1" 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Subject: Meeting Announcement: M3S/MATC October Meeting Correction 10, 23 -- Date: Wed, 15 Oct 2008 12:17:26 -0500 10, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150E19D-at-MCCRONEMSG.tmg.mccrone.com} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Meeting Announcement: M3S/MATC October Meeting Correction 10, 23 -- Thread-Index: Acku6eTNU1owUh5LTzmRpffwMs/5Jw== 10, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 15 Oct 2008 17:18:05.0037 (UTC) FILETIME=[FC3529D0:01C92EE9] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9FHI571005098 ==============================End of - Headers==============================
Imaging Scientist - St. Jude Children's Research Hospital.
Currently, St. Jude Children's Research Hospital has an opening for an Imaging Scientist (Job Number 16704) in the Cellular Imaging Department.
The successful candidate would be responsible for assisting faculty, postdoctoral researchers, staff, and collaborators in advance microscopy techniques and methodologies, including laser scanning and spinning disk confocal microscopy, wide-field microscopy, live cell imaging, FRET, FLIM, FCS, FRAP and TIRF, as well as routine maintenance of the instrumentation infrastructure.
Requirements:
A Bachelor's degree in an appropriate scientific field plus a minimum of ten (10) years (post-degree) of relevant and productive work experience is required OR,
A Master's degree in an appropriate scientific field plus a minimum of nine (9) years (post-degree) of relevant and productive work experience is required OR,
A PhD in an appropriate scientific field plus five (5) years (post-degree) of relevant and productive work experience is required.
To apply please visit our Web site http://www.stjude.org/jobs
St. Jude Children's Research Hospital is an Equal Opportunity Employer.
-- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office (901) 495-2536 Cell (901) 603-3162 samuel.connell-at-stjude.org
==============================Original Headers============================== 15, 24 -- From Samuel.Connell-at-STJUDE.ORG Wed Oct 15 23:05:08 2008 15, 24 -- Received: from mgate2.stjude.org (mgate2.stjude.org [192.55.208.21]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9G45864014836 15, 24 -- for {Microscopy-at-Microscopy.com} ; Wed, 15 Oct 2008 23:05:08 -0500 15, 24 -- From: "Connell, Samuel" {Samuel.Connell-at-STJUDE.ORG} 15, 24 -- To: "Connell, Samuel" {Samuel.Connell-at-STJUDE.ORG} 15, 24 -- Date: Wed, 15 Oct 2008 23:05:05 -0500 15, 24 -- Subject: Imaging Scientist Position-St. Jude Children's Research Hospital 15, 24 -- Thread-Topic: Imaging Scientist Position-St. Jude Children's Research 15, 24 -- Hospital 15, 24 -- Thread-Index: Acku5wFaUb1fyzR9R6i+oMw4ZYehyQACAMjgABVWjqI= 15, 24 -- Message-ID: {C51C27A1.5C96%Samuel.Connell-at-stjude.org} 15, 24 -- In-Reply-To: {73FDFAB4ECA0D442AD9F062FAF4E476346B29BD0AC-at-COLUMBO.eservices.virginia.edu} 15, 24 -- Accept-Language: en-US 15, 24 -- Content-Language: en 15, 24 -- X-MS-Has-Attach: 15, 24 -- X-MS-TNEF-Correlator: 15, 24 -- acceptlanguage: en-US 15, 24 -- Content-Type: text/plain; charset="iso-8859-1" 15, 24 -- MIME-Version: 1.0 15, 24 -- X-SEF-SJMEMMS4: 1 15, 24 -- X-SEF-Processed: 6_0_1_111__2008_10_15_23_05_08 15, 24 -- Content-Transfer-Encoding: 8bit 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9G45864014836 ==============================End of - Headers==============================
Is it possible to safely analyze a substance suspected to be gunpowder with EDS in an SEM?
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 8, 31 -- From mmcheath-at-syr.edu Thu Oct 16 17:48:24 2008 8, 31 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9GMmMra023515 8, 31 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:48:23 -0500 8, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 31 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9GMmITs027929 8, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 8, 31 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 18:48:19 -0400 8, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 8, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 8, 31 -- 8.1.291.1; Thu, 16 Oct 2008 18:48:19 -0400 8, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 8, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Thu, 16 Oct 2008 18:48:17 -0400 8, 31 -- Received: from 67.241.11.193 ([67.241.11.193]) by SUEXCL-02.ad.syr.edu 8, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 8, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Thu, 16 Oct 8, 31 -- 2008 22:48:18 +0000 8, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 8, 31 -- Date: Thu, 16 Oct 2008 18:48:17 -0400 8, 31 -- Subject: Identification of gunpowder 8, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {C51D3CF1.297AE%mmcheath-at-syr.edu} 8, 31 -- Thread-Topic: Identification of gunpowder 8, 31 -- Thread-Index: Ackv4Ud4vMoqkXTXIEC2jO5HMaz1+A== 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit 8, 31 -- X-OriginalArrivalTime: 16 Oct 2008 22:48:17.0995 (UTC) FILETIME=[481061B0:01C92FE1] 8, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7160:2.4.4,1.2.40,4.0.166 definitions=2008-10-17_01:2008-10-10,2008-10-16,2008-10-16 signatures=0 8, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
On Oct 16, 2008, at 3:48 PM, mmcheath-at-syr.edu wrote:
} Is it possible to safely analyze a substance suspected to be } gunpowder with } EDS in an SEM?
Dear Mike, A former colleague analyzed gunshot residue with a police department for many years, so it should be safe. Gunpowder does, however, contain some volatile elements, such as sulfur, which may end up depositing on the column. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Oct 16 19:44:22 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9H0iLXS008107 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 19:44:21 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 48662328B42 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:44:21 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 5D1D2328A09 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:44:20 -0700 (PDT) 6, 22 -- Message-Id: {C0532CD4-FB23-4AD7-AE5C-16E0C2FA3705-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200810162248.m9GMmiOj023662-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] Identification of gunpowder 6, 22 -- Date: Thu, 16 Oct 2008 17:44:20 -0700 6, 22 -- References: {200810162248.m9GMmiOj023662-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Isn't there a bit of difference between gunpowder, and gunshot residue? I got the impression the concern was whether localized heating caused by the beam could ignite the gunpowder. That is what peaked my interest in replies to the query. Did I misunderstand?
Darrell
tivol-at-caltech.edu wrote on 10/16/2008 08:45:33 PM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Oct 16, 2008, at 3:48 PM, mmcheath-at-syr.edu wrote: } } } Is it possible to safely analyze a substance suspected to be } } gunpowder with } } EDS in an SEM? } } } Dear Mike, } A former colleague analyzed gunshot residue with a police department } for many years, so it should be safe. Gunpowder does, however, } contain some volatile elements, such as sulfur, which may end up } depositing on the column. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 6, 22 -- From tivol-at-caltech.edu Thu Oct 16 19:44:22 2008 } 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing- } mail.its.caltech.edu [131.215.239.19]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m9H0iLXS008107 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 19:44:21 -0500 } 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) } 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 48662328B42 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:44: } 21 -0700 (PDT) } 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new } 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215. } caltech.edu [131.215.19.215]) } 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 5D1D2328A09 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:44: } 20 -0700 (PDT) } 6, 22 -- Message-Id: {C0532CD4-FB23-4AD7-AE5C-16E0C2FA3705-at-caltech.edu} } 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- In-Reply-To: {200810162248.m9GMmiOj023662-at-ns.microscopy.com} } 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 6, 22 -- Content-Transfer-Encoding: 7bit } 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) } 6, 22 -- Subject: Re: [Microscopy] Identification of gunpowder } 6, 22 -- Date: Thu, 16 Oct 2008 17:44:20 -0700 } 6, 22 -- References: {200810162248.m9GMmiOj023662-at-ns.microscopy.com} } 6, 22 -- X-Mailer: Apple Mail (2.929.2) } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 28 -- From milesd-at-us.ibm.com Thu Oct 16 19:53:43 2008 6, 28 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9H0rhjF021433 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 19:53:43 -0500 6, 28 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 6, 28 -- by e3.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id m9H0rgGj004232 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 20:53:42 -0400 6, 28 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 6, 28 -- by d01relay02.pok.ibm.com (8.13.8/8.13.8/NCO v9.1) with ESMTP id m9H0rg6c144588 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 20:53:42 -0400 6, 28 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 6, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id m9H0rgmU022843 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 20:53:42 -0400 6, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 6, 28 -- by d01av03.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id m9H0rgKv022838 6, 28 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 20:53:42 -0400 6, 28 -- In-Reply-To: {200810170045.m9H0jX0M009137-at-ns.microscopy.com} 6, 28 -- To: Microscopy-at-Microscopy.Com 6, 28 -- MIME-Version: 1.0 6, 28 -- Subject: Re: [Microscopy] Re: Identification of gunpowder 6, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 6, 28 -- Message-ID: {OF4EE40D9C.B7361DB6-ON852574E5.00048514-852574E5.0004E9A2-at-us.ibm.com} 6, 28 -- From: Darrell Miles {milesd-at-us.ibm.com} 6, 28 -- Date: Thu, 16 Oct 2008 20:53:42 -0400 6, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1|February 07, 2008) at 6, 28 -- 10/16/2008 20:53:43, 6, 28 -- Serialize complete at 10/16/2008 20:53:43 6, 28 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
You are correct, I am asking about gunpowder, not GSR. A little additional info...I don't have the sample myself. I have been asked by a PhD student in Anthropology if our department can determine if a substance she has is gunpowder or something else. I don't have any details, but I am assuming the sample is old.
Isn't there a bit of difference between gunpowder, and gunshot residue? I got the impression the concern was whether localized heating caused by the beam could ignite the gunpowder. That is what peaked my interest in replies to the query. Did I misunderstand?
Darrell
tivol-at-caltech.edu wrote on 10/16/2008 08:45:33 PM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Oct 16, 2008, at 3:48 PM, mmcheath-at-syr.edu wrote: } } } Is it possible to safely analyze a substance suspected to be } } gunpowder with } } EDS in an SEM? } } } Dear Mike, } A former colleague analyzed gunshot residue with a police department } for many years, so it should be safe. Gunpowder does, however, } contain some volatile elements, such as sulfur, which may end up } depositing on the column. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu } } } ==============================Original Headers============================== } 6, 22 -- From tivol-at-caltech.edu Thu Oct 16 19:44:22 2008 } 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing- } mail.its.caltech.edu [131.215.239.19]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m9H0iLXS008107 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 19:44:21 -0500 } 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) } 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 48662328B42 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:44: } 21 -0700 (PDT) } 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new } 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215. } caltech.edu [131.215.19.215]) } 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 5D1D2328A09 } 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:44: } 20 -0700 (PDT) } 6, 22 -- Message-Id: {C0532CD4-FB23-4AD7-AE5C-16E0C2FA3705-at-caltech.edu} } 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} } 6, 22 -- To: microscopy-at-microscopy.com } 6, 22 -- In-Reply-To: {200810162248.m9GMmiOj023662-at-ns.microscopy.com} } 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 6, 22 -- Content-Transfer-Encoding: 7bit } 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) } 6, 22 -- Subject: Re: [Microscopy] Identification of gunpowder } 6, 22 -- Date: Thu, 16 Oct 2008 17:44:20 -0700 } 6, 22 -- References: {200810162248.m9GMmiOj023662-at-ns.microscopy.com} } 6, 22 -- X-Mailer: Apple Mail (2.929.2) } ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 32 -- From mmcheath-at-syr.edu Thu Oct 16 20:10:44 2008 18, 32 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9H1AhdS002558 18, 32 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 20:10:43 -0500 18, 32 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 18, 32 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9H1AhgD007703 18, 32 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 18, 32 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Oct 2008 21:10:43 -0400 18, 32 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 18, 32 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 18, 32 -- 8.1.291.1; Thu, 16 Oct 2008 21:10:43 -0400 18, 32 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 18, 32 -- with Microsoft SMTPSVC(6.0.3790.3959); Thu, 16 Oct 2008 21:10:42 -0400 18, 32 -- Received: from 128.230.90.89 ([128.230.90.89]) by SUEXCL-02.ad.syr.edu 18, 32 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 18, 32 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Fri, 17 Oct 18, 32 -- 2008 01:10:43 +0000 18, 32 -- User-Agent: Microsoft-Entourage/12.12.0.080729 18, 32 -- Date: Thu, 16 Oct 2008 21:10:42 -0400 18, 32 -- Subject: FW: [Microscopy] Identification of gunpowder 18, 32 -- From: Michael Cheatham {mmcheath-at-syr.edu} 18, 32 -- To: {Microscopy-at-Microscopy.Com} 18, 32 -- Message-ID: {C51D5E52.297CF%mmcheath-at-syr.edu} 18, 32 -- Thread-Topic: [Microscopy] Identification of gunpowder 18, 32 -- Thread-Index: Ackv9SywzEByZ/CVzEqDGS6ZXxs+wg== 18, 32 -- In-Reply-To: {200810170057.m9H0vRgQ030648-at-ns.microscopy.com} 18, 32 -- MIME-Version: 1.0 18, 32 -- Content-Type: text/plain; charset="US-ASCII" 18, 32 -- Content-Transfer-Encoding: 7bit 18, 32 -- X-OriginalArrivalTime: 17 Oct 2008 01:10:42.0957 (UTC) FILETIME=[2D421BD0:01C92FF5] 18, 32 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7160:2.4.4,1.2.40,4.0.166 definitions=2008-10-17_01:2008-10-10,2008-10-16,2008-10-16 signatures=0 18, 32 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
True gun powder is black powder and it contains three classical ingredients, charcoal, sulfur and saltpeter. Most modern gunpowder is a smokeless and is a complicated mix of nitrocellulose, nitroglycerin (double base) and many other chemical ingredients. Flash powder is similar to black powder with the replacement of carbon with powdered aluminum.
At the rubber company I was employed at, we ran sulfur crystals in the SEM (25kv) without trouble (your milage...standard disclaimer) . Me? I'd wash a tiny amount of the material with droplet chloroform and let it evaporate an look for rhombs of elemental sulfur. Then I'd run the dried sample with the SEM/EDS.
Of course PLM could do the job. Charcoal is opaque, sulfur soluble in refractive index liquids, not so in water with wetting agent and has very high refractive index, saltpeter (look it up in your Merck, on line) has medium refractive index... Humm I hear a microchemical test coming...
Stay safe......... FrankKarl......... Linken-In Microscopy Group Manager
mmcheath-at-syr.edu
10/16/2008 06:57 To PM frank_karl-at-lincolnelectric.com cc
Please respond to Subject mmcheath-at-syr.edu [Microscopy] Identification of gunpowder
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Hi,
Is it possible to safely analyze a substance suspected to be gunpowder with EDS in an SEM?
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
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==============================Original Headers============================== 8, 31 -- From mmcheath-at-syr.edu Thu Oct 16 17:48:24 2008 8, 31 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9GMmMra023515 8, 31 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:48:23 -0500 8, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 31 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9GMmITs027929 8, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 8, 31 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 18:48:19 -0400 8, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 8, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 8, 31 -- 8.1.291.1; Thu, 16 Oct 2008 18:48:19 -0400 8, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 8, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Thu, 16 Oct 2008 18:48:17 -0400 8, 31 -- Received: from 67.241.11.193 ([67.241.11.193]) by SUEXCL-02.ad.syr.edu 8, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 8, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Thu, 16 Oct 8, 31 -- 2008 22:48:18 +0000 8, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 8, 31 -- Date: Thu, 16 Oct 2008 18:48:17 -0400 8, 31 -- Subject: Identification of gunpowder 8, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {C51D3CF1.297AE%mmcheath-at-syr.edu} 8, 31 -- Thread-Topic: Identification of gunpowder 8, 31 -- Thread-Index: Ackv4Ud4vMoqkXTXIEC2jO5HMaz1+A== 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit 8, 31 -- X-OriginalArrivalTime: 16 Oct 2008 22:48:17.0995 (UTC) FILETIME= [481061B0:01C92FE1] 8, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7160:2.4.4,1.2.40,4.0.166 definitions=2008-10-17_01:2008-10-10,2008-10-16,2008-10-16 signatures=0 8, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
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==============================Original Headers============================== 29, 22 -- From frank_karl-at-lincolnelectric.com Fri Oct 17 05:24:48 2008 29, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 29, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9HAOm2k026468 29, 22 -- for {microscopy-at-microscopy.com} ; Fri, 17 Oct 2008 05:24:48 -0500 29, 22 -- In-Reply-To: {200810162257.m9GMvDhb004448-at-ns.microscopy.com} 29, 22 -- Subject: Re: [Microscopy] Identification of gunpowder 29, 22 -- To: mmcheath-at-syr.edu, Microscopy-at-microscopy.com 29, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 29, 22 -- Message-ID: {OF0DE11C62.403A206B-ON852574E5.0037F9A5-852574E5.00392E15-at-lincolnelectric.com} 29, 22 -- Date: Fri, 17 Oct 2008 06:24:36 -0400 29, 22 -- From: Frank_Karl-at-lincolnelectric.com 29, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 29, 22 -- 07, 2008) at 10/17/2008 06:24:35 AM, 29, 22 -- CD-MIME complete at 10/17/2008 06:24:35 AM, 29, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 29, 22 -- 07, 2008) at 10/17/2008 06:24:35 AM, 29, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 29, 22 -- 07, 2008) at 10/17/2008 06:24:35 AM, 29, 22 -- Serialize complete at 10/17/2008 06:24:35 AM 29, 22 -- MIME-Version: 1.0 29, 22 -- Content-Type: text/plain; 29, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
Dear Mike, Years ago I analysed a mining explosive: encapsulated diesel fuel in urea. I was apprehensive, but nothing happened when the 20 kV beam hit the material. That said, you can analyse a very small amount, just a few grains, so the danger is minimized. Also, a gunpowder explosion is a rapid oxidation, and the material is in a vacuum with very limited access to oxygen. I'm not sure that an EDX analysis can run down a complex organic material such as modern gunpowder, but you can at least categorize it as possible gunpowder. Regards,
-----Original Message----- X-from: mmcheath-at-syr.edu [mailto:mmcheath-at-syr.edu] Sent: October 16, 2008 3:55 PM To: maryflet-at-interchange.ubc.ca
Hi,
Is it possible to safely analyze a substance suspected to be gunpowder with EDS in an SEM?
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 8, 31 -- From mmcheath-at-syr.edu Thu Oct 16 17:48:24 2008 8, 31 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9GMmMra023515 8, 31 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 17:48:23 -0500 8, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 31 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9GMmITs027929 8, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 8, 31 -- for {microscopy-at-microscopy.com} ; Thu, 16 Oct 2008 18:48:19 -0400 8, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 8, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 8, 31 -- 8.1.291.1; Thu, 16 Oct 2008 18:48:19 -0400 8, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 8, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Thu, 16 Oct 2008 18:48:17 -0400 8, 31 -- Received: from 67.241.11.193 ([67.241.11.193]) by SUEXCL-02.ad.syr.edu 8, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 8, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Thu, 16 Oct 8, 31 -- 2008 22:48:18 +0000 8, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 8, 31 -- Date: Thu, 16 Oct 2008 18:48:17 -0400 8, 31 -- Subject: Identification of gunpowder 8, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {C51D3CF1.297AE%mmcheath-at-syr.edu} 8, 31 -- Thread-Topic: Identification of gunpowder 8, 31 -- Thread-Index: Ackv4Ud4vMoqkXTXIEC2jO5HMaz1+A== 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit 8, 31 -- X-OriginalArrivalTime: 16 Oct 2008 22:48:17.0995 (UTC) FILETIME=[481061B0:01C92FE1] 8, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7160:2.4.4,1.2.40,4.0.166 definitions=2008-10-17_01:2008-10-10,2008-10-16,2008-10-16 signatures=0 8, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
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I regularly image high explosive powders - RDX, HMX, HNS - in the SEM, to identify crystal shapes and sizes.
We do not have a light element detector so no EDS - these things are mostly nitro groups with hydrogen and carbon.
I've never had anything "go" - but one time did see an HNS crystal start to "move" and melt a little, at high mag. Left it alone right away.
What saves the situation for me is that I use an aluminum stub (better heat conduction than graphite), gold-coat (lightly) the specimen to get better secondary electron yield for imaging, and use low accelerating voltages since I'm not trying to excite electrons for EDS.
Depending on how big your sample is, you may wish to start by seeing if it softens in acetone. Modern "smokeless" nitro powder will soften. Black powder - sulfur, charcoal, and potassium nitrate - probably will not.
If you do not have a light element detector, but do EDS anyway, and you see S and K, you may have black powder.
Regards, Andrew
At 05:49 PM 10/16/2008, you wrote:
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Does anyone happen to have an old Philips CM TEM specimen rod hanging about the lab, or parts? I am in need of the grid clamping ring. The clamping device would do, but all I really need is the ring (or a couple/three for when I drop one). This is Philips part # 5322 695 14978. Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Two weeks are remaining for submitting abstracts to the MRS Spring 2008 meeting Symposium JJ on Nanoscale Electromechanics and Piezoresponse Force Microscopy. The symposium will feature recent advances in experimental methods and theoretical understanding of electromechanical coupling on the nanoscale in polar, ferroelectric, macromolecular, and biological systems. The goal of this symposium is to bring together the experts from biology, materials science, and organic chemistry communities interested in electromechanical processes, scanning probe microscopists developing the experimental pathway for probing nanoelectromechanics, and theorists interested in fundamental mechanisms of electromechanical energy conversion, to formulate the outstanding research needs, grand challenges, applications, and development pathway for this rapidly emerging field.
The symposium information is available on line at http://www.mrs.org/s_mrs/sec.asp?CID=14465&DID=211517
The information on the PFM and materials of PFM workshops can be found in the section "Knowledge Base" at http://imaging.ornl.gov
Looking forward to seeing you in San Francisco
Sergei V. Kalinin
Team Leader, Force-Based Scanning Probe Microscopy Center for Nanophase Materials Sciences Oak Ridge National Laboratory Oak Ridge, TN 37922 URL: http://imaging.ornl.gov
Adjunct Associate Professor Department of Materials Science and Engineering University of Tennessee, Knoxville
==============================Original Headers============================== 6, 24 -- From sergei2-at-ornl.gov Fri Oct 17 14:15:11 2008 6, 24 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9HJFA31008868 6, 24 -- for {microscopy-at-microscopy.com} ; Fri, 17 Oct 2008 14:15:11 -0500 6, 24 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 6, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 6, 24 -- with ESMTP id {0K8W00F2EDHASX-at-emroute1.ornl.gov} for 6, 24 -- microscopy-at-microscopy.com; Fri, 17 Oct 2008 15:15:10 -0400 (EDT) 6, 24 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 6, 24 -- (PMDF V6.4 #31561) id {0K8W00G01DHA8D-at-emroute1.ornl.gov} for 6, 24 -- microscopy-at-microscopy.com; Fri, 17 Oct 2008 15:15:10 -0400 (EDT) 6, 24 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 6, 24 -- by emroute1.ornl.gov (PMDF V6.4 #31561) 6, 24 -- with ESMTP id {0K8W008NIDHAGR-at-emroute1.ornl.gov} for 6, 24 -- microscopy-at-microscopy.com; Fri, 17 Oct 2008 15:15:10 -0400 (EDT) 6, 24 -- Date: Fri, 17 Oct 2008 15:15:09 -0400 6, 24 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 6, 24 -- Subject: Piezoresponse Force Microscopy - MRS Spring meeting 6, 24 -- To: microscopy-at-microscopy.com, "Sergei V. Kalinin" {sergei2-at-ornl.gov} 6, 24 -- Message-id: {48F8E43D.9050503-at-ornl.gov} 6, 24 -- MIME-version: 1.0 6, 24 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 6, 24 -- Content-transfer-encoding: 7bit 6, 24 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) ==============================End of - Headers==============================
A colleague would like to TUNEL-label ultra-thin Epon-Araldite sections for EM. Does anyone have, or know of, any successful protocols that I may relay to her? I believe her tissue may have been fixed in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.4 (no potassium), post-fixed with osmium and en bloc-stained with uranyl acetate.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
Many thanks to all, I already have a source of the specimen rod clamping ring I need. Wow. I didn't expect such speed on a Friday afternoon.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 23 -- From oshel1pe-at-cmich.edu Fri Oct 17 14:37:03 2008 3, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9HJb3tJ003610 3, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Oct 2008 14:37:03 -0500 3, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 23 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9HJb289018892 3, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Oct 2008 15:37:02 -0400 3, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 23 -- Fri, 17 Oct 2008 15:37:01 -0400 3, 23 -- Mime-Version: 1.0 3, 23 -- Message-Id: {f0624080ac51e9996cdec-at-[141.209.160.249]} 3, 23 -- Date: Fri, 17 Oct 2008 15:36:59 -0400 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 23 -- Subject: Philips clamping ring. Hurrah 3, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 23 -- X-OriginalArrivalTime: 17 Oct 2008 19:37:01.0849 (UTC) FILETIME=[BA293890:01C9308F] 3, 23 -- X-Canit-CHI2: 0.00 3, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 23 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 3, 23 -- X-CanItPRO-Stream: default 3, 23 -- X-Canit-Stats-ID: 4096695 - 97ca28ef0b2b 3, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Organization: McLean Hospital/Harvard Medical School
Title-Subject: [Filtered] Post-embedding immunogold staining on brain tissue for TEM: High background
Question: Post-embedding immunogold staining on brain tissue for TEM
I am working on post-embedding immunogold staining to recognize synaptic vesicles from brain tissue using TEM. EM technician in our facility have processed the tissue in following way: 1. wash with PBS 2. 1% Osmium 1 hour 3. wash with H2O 4. Dehydration 5. Embedding 6. Sectioning and placed on the nickel grids.
I have tried immunogold staining using following method: 1. 1% Sodium metaperiodate treatment 2. blocking 1 hour in 10% BSA, 3% goat serum, 0.1% fish gelatin, 0.02% triton-X 3. primary antibody (10 times higher concentration than usual) in 2% goat serum, 0.1% fish gelatin 4. wash with PBS, 0.5% BSA, 0.5% tween 20 5. secondary Ab (5 nm colloidal gold conjugate, Ted Pella) 1:20, 1 hour 30 min 6. wash with PBS, 0.5M NaCl, 0.5 % BSA. 0.5% tween 20 7. 1% glutaraldehyde fixation for 5 min. 8. wash with H2O 9. Uranyl-lead staining
Results are following: 1. Ultrastructure was well preserved 2. Nonspecific gold particles were observed in the area where there was no tissue pieces. 3. There seem to be nonspecific gold particle within the tissue as well.
As of now, due to the high background, I am not certain that I have any specific staining. If any of you have a good way to stain post-embedded tissue without background, I would greatly appreciate your advice...
Thank you so much!
Chee-Yeun
Chee-Yeun Chung, Ph.D. Instructor in Psychiatry McLean Hosptial/Harvard Medical School
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Email: susan.vanhorn-at-sunysb.edu Name: Susan C. Van Horn
Organization: SUNY-at-StonyBrook
Title-Subject: [Filtered] Spur resin
Question: I have been using toluidine blue to stain "thick" sections(1um)of embedded brain tissue in spurrs resin....am not happy with the quality of the histology/stain.....can someone suggest a better stain/method for material embedded in spurrs???? thanks sue
You fail to give us all the essential information on your technique. I assume you pre-fixed the synaptic vesicles with an aldehyde prior to giving them to the EM technician - if so, what one(s) used and at what concentration may be influencing the immunoreactivity. Second, you don't mention which resin you are trying - this is a key variable in post-embedding EM immunocytochemistry. I have had success with several resins but often one resin is better than another for a particular antigen for reasons that are not clear to me. I often try 3-4 resins for every EM immuno project (Lowicryl K4M, HM20, LR Gold, LR White). Your use of sodium metaperiodate suggests you might be using an epoxy resin like Epon. If so, you chances for success are even less than normal. LR White might be a better choice. Even better, skip the osmium and use LR Gold. You don't say what your gold conjugate is but it better be a goat anti-something based on your blocking protocol. Finally, you don't indicate whether there is any evidence for the primary working at the LM level on fixed tissues. My main comment is that you are trying a fixation and immunolabeling protocol that will work for only a few antigens. Is there any evidence that your protocol works for this particular antibody (i.e., a published paper or LM results)? Post-embedding EM labeling is one the toughest immunocytochemistry techniques around - it works best for high abundance antigens. It rarely works on the first try in my experience. One needs to optimize the fixation, resin, and antibody staining concentration and duration for each antibody. Good luck in your efforts. Tom
-----Original Message----- X-from: cychung-at-mclean.harvard.edu [mailto:cychung-at-mclean.harvard.edu] Sent: Sunday, October 19, 2008 3:20 AM To: Phillips, Thomas E.
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Organization: McLean Hospital/Harvard Medical School
Title-Subject: [Filtered] Post-embedding immunogold staining on brain tissue for TEM: High background
Question: Post-embedding immunogold staining on brain tissue for TEM
I am working on post-embedding immunogold staining to recognize synaptic vesicles from brain tissue using TEM. EM technician in our facility have processed the tissue in following way: 1. wash with PBS 2. 1% Osmium 1 hour 3. wash with H2O 4. Dehydration 5. Embedding 6. Sectioning and placed on the nickel grids.
I have tried immunogold staining using following method: 1. 1% Sodium metaperiodate treatment 2. blocking 1 hour in 10% BSA, 3% goat serum, 0.1% fish gelatin, 0.02% triton-X 3. primary antibody (10 times higher concentration than usual) in 2% goat serum, 0.1% fish gelatin 4. wash with PBS, 0.5% BSA, 0.5% tween 20 5. secondary Ab (5 nm colloidal gold conjugate, Ted Pella) 1:20, 1 hour 30 min 6. wash with PBS, 0.5M NaCl, 0.5 % BSA. 0.5% tween 20 7. 1% glutaraldehyde fixation for 5 min. 8. wash with H2O 9. Uranyl-lead staining
Results are following: 1. Ultrastructure was well preserved 2. Nonspecific gold particles were observed in the area where there was no tissue pieces. 3. There seem to be nonspecific gold particle within the tissue as well.
As of now, due to the high background, I am not certain that I have any specific staining. If any of you have a good way to stain post-embedded tissue without background, I would greatly appreciate your advice...
Thank you so much!
Chee-Yeun
Chee-Yeun Chung, Ph.D. Instructor in Psychiatry McLean Hosptial/Harvard Medical School
If pre-embedding labeling works, why don't you label and then osmicate and embed? At the very least you good label with the primary before embedding but this is probably a case where you can easily do both. Remember the sensitivity of the labeling depends on gold size. Use 10 nm whenever possible since it is much more efficient than 15 and 20 nm.
Thomas E. Phillips, Ph.D. Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall Biological Sciences University of Missouri Columbia, MO 65211-7400 573-882-4712 (voice) 573-882-0123 (fax)
X-from: CheeYeun Chung [mailto:cychung-at-mclean.harvard.edu] Sent: Sunday, October 19, 2008 11:19 PM To: Phillips, Thomas E. Cc: microscopy-at-microscopy.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both cychung-at-mclean.harvard.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Organization: McLean Hospital/Harvard Medical School
Title-Subject: [Filtered] Post-embedding immunogold staining on brain tissue for TEM: High background
Question: Post-embedding immunogold staining on brain tissue for TEM
I am working on post-embedding immunogold staining to recognize synaptic vesicles from brain tissue using TEM. EM technician in our facility have processed the tissue in following way: 1. wash with PBS 2. 1% Osmium 1 hour 3. wash with H2O 4. Dehydration 5. Embedding 6. Sectioning and placed on the nickel grids.
I have tried immunogold staining using following method: 1. 1% Sodium metaperiodate treatment 2. blocking 1 hour in 10% BSA, 3% goat serum, 0.1% fish gelatin, 0.02% triton-X 3. primary antibody (10 times higher concentration than usual) in 2% goat serum, 0.1% fish gelatin 4. wash with PBS, 0.5% BSA, 0.5% tween 20 5. secondary Ab (5 nm colloidal gold conjugate, Ted Pella) 1:20, 1 hour 30 min 6. wash with PBS, 0.5M NaCl, 0.5 % BSA. 0.5% tween 20 7. 1% glutaraldehyde fixation for 5 min. 8. wash with H2O 9. Uranyl-lead staining
Results are following: 1. Ultrastructure was well preserved 2. Nonspecific gold particles were observed in the area where there was no tissue pieces. 3. There seem to be nonspecific gold particle within the tissue as well.
As of now, due to the high background, I am not certain that I have any specific staining. If any of you have a good way to stain post-embedded tissue without background, I would greatly appreciate your advice...
Thank you so much!
Chee-Yeun
Chee-Yeun Chung, Ph.D. Instructor in Psychiatry McLean Hosptial/Harvard Medical School
==============================Original Headers============================== 13, 12 -- From zaluzec-at-microscopy.com Sun Oct 19 03:18:23 2008 13, 12 -- Received: from [172.28.162.230] (ppp-82-85-24-66.b2b.tiscali.it [82.85.24.66] (may be forged)) 13, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9J8IKwn022958 13, 12 -- for {microscopy-at-microscopy.com} ; Sun, 19 Oct 2008 03:18:22 -0500 13, 12 -- Mime-Version: 1.0 13, 12 -- Message-Id: {p06240800c5209dba1f78-at-[206.69.208.26]} 13, 12 -- Date: Sun, 19 Oct 2008 03:18:21 -0500 13, 12 -- To: microscopy-at-microscopy.com 13, 12 -- From: cychung-at-mclean.harvard.edu (by way of MicroscopyListserver) 13, 12 -- Subject: viaWWW: Post-embedding immunogold staining on brain tissue for 13, 12 -- TEM: High background 13, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Chee-Yeun Chung, Ph.D. Instructor in Psychiatry McLean Hosptial/Harvard Medical School 115 Mill St. Belmont, MA 02478 Tel) 617-855-2682 Fax) 617-855-2522
==============================Original Headers============================== 41, 25 -- From PhillipsT-at-missouri.edu Mon Oct 20 08:07:46 2008 41, 25 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 41, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9KD7jua019797 41, 25 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 08:07:45 -0500 41, 25 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 41, 25 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 20 Oct 2008 08:07:45 -0500 41, 25 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 41, 25 -- Mon, 20 Oct 2008 08:07:45 -0500 41, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 41, 25 -- Content-class: urn:content-classes:message 41, 25 -- MIME-Version: 1.0 41, 25 -- Content-Type: text/plain; 41, 25 -- charset="iso-8859-1" 41, 25 -- Subject: [Microscopy] viaWWW: Post-embedding immunogold staining on brain tissue for 41, 25 -- Date: Mon, 20 Oct 2008 08:07:43 -0500 41, 25 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD052427E2-at-UM-XMAIL06.um.umsystem.edu} 41, 25 -- X-MS-Has-Attach: 41, 25 -- X-MS-TNEF-Correlator: 41, 25 -- Thread-Topic: [Microscopy] viaWWW: Post-embedding immunogold staining on brain tissue for 41, 25 -- Thread-Index: Ackya4iLophQh+PTTJuT+2A5I2zBfgAP3lYwAAJwanA= 41, 25 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 41, 25 -- To: {microscopy-at-microscopy.com} 41, 25 -- X-OriginalArrivalTime: 20 Oct 2008 13:07:45.0386 (UTC) FILETIME=[D7DD0CA0:01C932B4] 41, 25 -- Content-Transfer-Encoding: 8bit 41, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9KD7jua019797 ==============================End of - Headers==============================
Have you tried adding 1% sodium tetraborate (borax) to the toluidine blue? You also need to heat the section while staining. This works for me, although I mainly work on plant tissue and I cannot vouch for its efficacy with the new Spurr formulations. Apologies if these suggestions are too obvious.
Regards
Carol
-----Original Message----- X-from: susan.vanhorn-at-sunysb.edu [mailto:susan.vanhorn-at-sunysb.edu] Sent: 19 October 2008 09:27 To: carol.evered-at-warwick.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both susan.vanhorn-at-sunysb.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: susan.vanhorn-at-sunysb.edu Name: Susan C. Van Horn
Organization: SUNY-at-StonyBrook
Title-Subject: [Filtered] Spur resin
Question: I have been using toluidine blue to stain "thick" sections(1um)of embedded brain tissue in spurrs resin....am not happy with the quality of the histology/stain.....can someone suggest a better stain/method for material embedded in spurrs???? thanks sue
There are a great many papers published on staining "thick" sections. The ones that give good results, at least in the hands of the authors, are long and very complicated. While slides stained with such methods look very nice, they still are not as pretty as a proper hematoxylin and eosin. Thus, the popularity of toluidine blue in sodium metaborate, simple and effective. If you feel that you must try something better I will send you a partial listing of such "improved" methods.
Geoff
susan.vanhorn-at-sunysb.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both susan.vanhorn-at-sunysb.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: susan.vanhorn-at-sunysb.edu } Name: Susan C. Van Horn } } Organization: SUNY-at-StonyBrook } } Title-Subject: [Filtered] Spur resin } } Question: I have been using toluidine blue to stain "thick" } sections(1um)of embedded brain tissue in spurrs resin....am not happy } with the quality of the histology/stain.....can someone suggest a } better stain/method for material embedded in spurrs???? } thanks } sue } } Login Host: 24.45.178.10 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Sun Oct 19 03:18:44 2008 } 6, 11 -- Received: from [172.28.162.230] (ppp-82-85-24-66.b2b.tiscali.it [82.85.24.66] (may be forged)) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9J8Ig43023358 } 6, 11 -- for {microscopy-at-microscopy.com} ; Sun, 19 Oct 2008 03:18:43 -0500 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240801c5209dd525e3-at-[172.28.162.230]} } 6, 11 -- Date: Sun, 19 Oct 2008 03:18:43 -0500 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: susan.vanhorn-at-sunysb.edu (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Spur resin } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 28 -- From mcauliff-at-umdnj.edu Mon Oct 20 09:18:23 2008 9, 28 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9KEINuN016920 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 09:18:23 -0500 9, 28 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 9, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id C12104BE3C 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 10:18:22 -0400 (EDT) 9, 28 -- Received: from umdnj.edu (imail.UMDNJ.EDU [130.219.34.129]) 9, 28 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 6282D4BE66 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 10:18:21 -0400 (EDT) 9, 28 -- Received: from ([10.32.15.167]) 9, 28 -- by imail.umdnj.edu with ESMTP id 5502050.29227572; 9, 28 -- Mon, 20 Oct 2008 10:18:01 -0400 9, 28 -- MIME-version: 1.0 9, 28 -- Content-transfer-encoding: 7BIT 9, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 9, 28 -- by umduwc01.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 9, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0K9100BW4JQ1IKF0-at-umduwc01.umdnj.edu} for 9, 28 -- microscopy-at-microscopy.com; Mon, 20 Oct 2008 10:18:01 -0400 (EDT) 9, 28 -- Message-id: {48FC9355.8090206-at-umdnj.edu} 9, 28 -- Date: Mon, 20 Oct 2008 10:19:01 -0400 9, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 28 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 9, 28 -- To: susan.vanhorn-at-sunysb.edu, microscopy-at-microscopy.com 9, 28 -- Subject: Re: [Microscopy] viaWWW: Spur resin 9, 28 -- References: {200810190819.m9J8Jv0I026333-at-ns.microscopy.com} 9, 28 -- In-reply-to: {200810190819.m9J8Jv0I026333-at-ns.microscopy.com} ==============================End of - Headers==============================
I have a client who is using Z-fix that is having problems with autofluoresence. Do this stuff glow by itself?
If anybody has experience with this?
Thanks in advance,
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 9, 25 -- From vapatpxs-at-yahoo.com Mon Oct 20 15:43:44 2008 9, 25 -- Received: from n20.bullet.mail.mud.yahoo.com (n20.bullet.mail.mud.yahoo.com [68.142.206.147]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9KKhiqM028858 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 15:43:44 -0500 9, 25 -- Received: from [209.191.108.96] by n20.bullet.mail.mud.yahoo.com with NNFMP; 20 Oct 2008 20:43:43 -0000 9, 25 -- Received: from [68.142.201.70] by t3.bullet.mud.yahoo.com with NNFMP; 20 Oct 2008 20:43:43 -0000 9, 25 -- Received: from [127.0.0.1] by omp422.mail.mud.yahoo.com with NNFMP; 20 Oct 2008 20:43:43 -0000 9, 25 -- X-Yahoo-Newman-Property: ymail-3 9, 25 -- X-Yahoo-Newman-Id: 363853.5892.bm-at-omp422.mail.mud.yahoo.com 9, 25 -- Received: (qmail 74411 invoked by uid 60001); 20 Oct 2008 20:43:42 -0000 9, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 25 -- s=s1024; d=yahoo.com; 9, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 9, 25 -- b=Z3E2r/C3k8tlJJNkCu1YAOZ2yJCg+o1tJOFf4P6tMb+6+LLLDcmyYilZBLfh7+abicf/hG3B9yKF9mh8B6SmSyHqHcj45pPcdcl+2Nt2hG+RDJm70U9wWKgiHZFft5kZUBk6S0lGn3P+QcoLhAqVoRbjXuQiDAzAtO/Hae0kW3k=; 9, 25 -- X-YMail-OSG: 4rF0N2cVM1kqTL18TerXjisGgPy5DNkKTagvOL30BR73BaR40mQfgwGo4DWEvZXYJbgpW1mpjO5DUJZN.oy_8V9qyXPBxHr7kBlp0khJeBcQJQr0N2vqreVC5gCp4mEmyvamLkjfBUSXJ7LVYW7JffCXDTY- 9, 25 -- Received: from [132.239.85.200] by web46109.mail.sp1.yahoo.com via HTTP; Mon, 20 Oct 2008 13:43:42 PDT 9, 25 -- X-Mailer: YahooMailWebService/0.7.218.2 9, 25 -- Date: Mon, 20 Oct 2008 13:43:42 -0700 (PDT) 9, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 9, 25 -- Reply-To: vapatpxs-at-yahoo.com 9, 25 -- Subject: Does Z-fix autofluoresce 9, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; charset=us-ascii 9, 25 -- Message-ID: {798310.72833.qm-at-web46109.mail.sp1.yahoo.com} ==============================End of - Headers==============================
On Oct 16, 2008, at 6:10 PM, mmcheath-at-syr.edu wrote:
} You are correct, I am asking about gunpowder, not GSR. A little } additional } info...I don't have the sample myself. I have been asked by a PhD } student } in Anthropology if our department can determine if a substance she } has is } gunpowder or something else. I don't have any details, but I am } assuming } the sample is old. } } TIA } Mike } } } } Isn't there a bit of difference between gunpowder, and gunshot } residue? I } got the impression the concern was whether localized heating caused } by the } beam could ignite the gunpowder. That is what peaked my interest in } replies to the query. Did I misunderstand? } } Darrell } } } } } } } Is it possible to safely analyze a substance suspected to be } } } gunpowder with } } } EDS in an SEM? } } } } } } Dear Mike, } } A former colleague analyzed gunshot residue with a police } } department } } for many years, so it should be safe. Gunpowder does, however, } } contain some volatile elements, such as sulfur, which may end up } } depositing on the column. } Dear Mike, I had not considered the possibility of beam heating causing a chemical reaction due to both the small amount of heat produced by the beam, the upper limit of which can be estimated from the energy loss, and the small amount of irradiated specimen, which would not produce a significant amount of pressure even if the entire specimen were to be reacted instantaneously--assuming that only a small amount of powder was loaded into the scope. Since the GSR consists of many of the same elements as the unfired gunpowder, I did not distinguish between the two. Although I do not have experience with SEM, I do not think you would be risking damage to your instrument by attempting to analyze a substance that might be gunpowder, but I will defer to those who know more. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Mon Oct 20 18:50:08 2008 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9KNo8TN020326 4, 22 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 18:50:08 -0500 4, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 4, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 00C2166E0A86 4, 22 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 16:50:07 -0700 (PDT) 4, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 4, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 4, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id B926766E09D8 4, 22 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 16:50:06 -0700 (PDT) 4, 22 -- Message-Id: {174FF67F-9BA2-4675-8DA5-C5C5D3AFD1EE-at-caltech.edu} 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- In-Reply-To: {200810170110.m9H1AoFD002683-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 4, 22 -- Subject: Re: [Microscopy] FW: Identification of gunpowder 4, 22 -- Date: Mon, 20 Oct 2008 16:50:05 -0700 4, 22 -- References: {200810170110.m9H1AoFD002683-at-ns.microscopy.com} 4, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
tivol-at-caltech.edu wrote on 10/20/2008 07:50:54 PM:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } On Oct 16, 2008, at 6:10 PM, mmcheath-at-syr.edu wrote: } } } You are correct, I am asking about gunpowder, not GSR. A little } } additional } } info...I don't have the sample myself. I have been asked by a PhD } } student } } in Anthropology if our department can determine if a substance she } } has is } } gunpowder or something else. I don't have any details, but I am } } assuming } } the sample is old. } } } } TIA } } Mike } } } } } } } } Isn't there a bit of difference between gunpowder, and gunshot } } residue? I } } got the impression the concern was whether localized heating caused } } by the } } beam could ignite the gunpowder. That is what peaked my interest in } } replies to the query. Did I misunderstand? } } } } Darrell } } } } } } } } } } } Is it possible to safely analyze a substance suspected to be } } } } gunpowder with } } } } EDS in an SEM? } } } } } } } } } Dear Mike, } } } A former colleague analyzed gunshot residue with a police } } } department } } } for many years, so it should be safe. Gunpowder does, however, } } } contain some volatile elements, such as sulfur, which may end up } } } depositing on the column. } } } Dear Mike, } I had not considered the possibility of beam heating causing a } chemical reaction due to both the small amount of heat produced by the } beam, the upper limit of which can be estimated from the energy loss, } and the small amount of irradiated specimen, which would not produce a } significant amount of pressure even if the entire specimen were to be } reacted instantaneously--assuming that only a small amount of powder } was loaded into the scope. Since the GSR consists of many of the same } elements as the unfired gunpowder, I did not distinguish between the } two. Although I do not have experience with SEM, I do not think you } would be risking damage to your instrument by attempting to analyze a } substance that might be gunpowder, but I will defer to those who know } more. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu }
While I had not considered the relative lack of oxygen to sustain the reaction, it was the mess that would have been made inside the SEM, not any dangerous rise in pressure from a small sample. I have seen a SEM basically rendered useless, due to the mess that coated the insides of the vacuum system. I have also seen the heat from the beam, especially a strong beam for EDS, damage small samples quit a bit.
It has been mentioned earlier, but I have seen chemical analysis (identification) done on small samples, on the stage of a Polarized Light Microscope. I was in a class on PLM here at work, taught by Walter McCrone. Demonstrating some of the capabilities of PLM, he did the chemical analysis. It was over my head, but he demonstrated what could be done for material identification.
Good luck in your quest. Darrell
==============================Original Headers============================== 8, 28 -- From milesd-at-us.ibm.com Mon Oct 20 19:32:58 2008 8, 28 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9L0WwlB004898 8, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 20 Oct 2008 19:32:58 -0500 8, 28 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 8, 28 -- by e2.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id m9L0WtJM016865 8, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 20 Oct 2008 20:32:55 -0400 8, 28 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 8, 28 -- by d01relay02.pok.ibm.com (8.13.8/8.13.8/NCO v9.1) with ESMTP id m9L0WtdH103200 8, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 20 Oct 2008 20:32:55 -0400 8, 28 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 8, 28 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id m9L0WtF8018531 8, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 20 Oct 2008 20:32:55 -0400 8, 28 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 8, 28 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id m9L0WtYk018525 8, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 20 Oct 2008 20:32:55 -0400 8, 28 -- In-Reply-To: {200810202350.m9KNost2021369-at-ns.microscopy.com} 8, 28 -- To: Microscopy-at-Microscopy.Com 8, 28 -- MIME-Version: 1.0 8, 28 -- Subject: Re: [Microscopy] Re: FW: Identification of gunpowder 8, 28 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 8, 28 -- Message-ID: {OFA698C093.EC2A09B8-ON852574E9.0000C7EB-852574E9.00030277-at-us.ibm.com} 8, 28 -- From: Darrell Miles {milesd-at-us.ibm.com} 8, 28 -- Date: Mon, 20 Oct 2008 20:32:55 -0400 8, 28 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1|February 07, 2008) at 8, 28 -- 10/20/2008 20:32:57, 8, 28 -- Serialize complete at 10/20/2008 20:32:57 8, 28 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
Forgive my ignorance, but doesn't gunpowder contain its own oxidizer? Depending on how intimately mixed the phases are, it may not matter if it is in a vacuum or not.
Based on a demo I saw some time ago, I believe that gunpowder will generally just burn rapidly if not confined. To detonate, it needs to be kept in a confined volume. If I remember correctly the optimal grain size was some 10s of microns. If so, you may wind up heating only one of the phases at a time with an SEM beam and no reaction will take place. However even if it doesn't burn, decomposition products (e.g. sulfur) may still crud up the SEM chamber.
Cheers, Henk
At 08:34 PM 10/20/2008, milesd-at-us.ibm.com wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility (614) 292-0674 040 Fontana Labs, 116 W. 19th Ave www.ceof.ohio-state.edu
==============================Original Headers============================== 11, 26 -- From colijn.1-at-osu.edu Mon Oct 20 20:05:24 2008 11, 26 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9L15O2t024186 11, 26 -- for {microscopy-at-microscopy.com} ; Mon, 20 Oct 2008 20:05:24 -0500 11, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 11, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31224) 11, 26 -- id {01N0YO09QWY88WW4I1-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 11, 26 -- Mon, 20 Oct 2008 21:05:23 -0400 (EDT) 11, 26 -- Received: from HOC3.osu.edu 11, 26 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 11, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31224) 11, 26 -- with ESMTPA id {01N0YO074R868X8OBW-at-ecr6.ohio-state.edu} ; Mon, 11, 26 -- 20 Oct 2008 21:05:20 -0400 (EDT) 11, 26 -- Date: Mon, 20 Oct 2008 21:05:18 -0400 11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 26 -- Subject: Re: [Microscopy] FW: Identification of gunpowder 11, 26 -- In-reply-to: {200810210034.m9L0Y6J6007365-at-ns.microscopy.com} 11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 26 -- To: milesd-at-us.ibm.com 11, 26 -- Cc: microscopy-at-microscopy.com, mmcheath-at-syr.edu 11, 26 -- Message-id: {7.0.1.0.2.20081020204946.0447c208-at-osu.edu} 11, 26 -- MIME-version: 1.0 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 26 -- References: {200810210034.m9L0Y6J6007365-at-ns.microscopy.com} ==============================End of - Headers==============================
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Email: anna.elsukova-at-uni-due.de Name: Anna Elsukova
Organization: University Duisburg-Essen
Title-Subject: [Filtered] TEM alignment in nanoprobe mode
Question: Could someone recommend some good manual or any source on nanoprobe alignment? Actually, I don't find microscope's manuals satisfactory. What I need is the description of the whole procedure plus (in the best case) rays diagrams (something like on www.rodenburg.org).
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Our lab has American Optical Model 925 and 935 Microtome knife sharpners and couple of microtome knives we want to sharpen. We need the fine and coarse abrasive solution that you need to sharpen the knives, does anybody know good source for these solution or an alternative?
Vendors please feel free to contact me offline!
Thanks,
Raj.
Neeraj V. Gohad, PhD Postdoctoral Fellow, Okeanos Research Group Department of Biological Sciences 132 Long Hall,Clemson University Clemson, SC-29634 864-656-3597 neerajg-at-clemson.edu
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Hi all,
Forgive my ignorance, but doesn't gunpowder contain its own oxidizer? Depending on how intimately mixed the phases are, it may not matter if it is in a vacuum or not.
Based on a demo I saw some time ago, I believe that gunpowder will generally just burn rapidly if not confined. To detonate, it needs to be kept in a confined volume. If I remember correctly the optimal grain size was some 10s of microns. If so, you may wind up heating only one of the phases at a time with an SEM beam and no reaction will take place. However even if it doesn't burn, decomposition products (e.g. sulfur) may still crud up the SEM chamber.
Don't know if there is enough energy available to initiate rapid decomposition, but agree that gunpowder does not require an external oxygen source. Also agree that as a "primary" explosive compound, unless confined, will simply burn rapidly, not detonate. Now, something like ammonium tri-iodide would be a different matter! That would really wreck an SEM...
Woody
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==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Tue Oct 21 07:06:36 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9LC6ZT8022108 2, 28 -- for {microscopy-at-microscopy.com} ; Tue, 21 Oct 2008 07:06:36 -0500 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.11604186; 2, 28 -- Tue, 21 Oct 2008 08:06:15 -0400 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Tue, 21 Oct 2008 08:06:14 -0400 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Subject: RE: [Microscopy] Re: FW: Identification of gunpowder 2, 28 -- Date: Tue, 21 Oct 2008 08:06:14 -0400 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7033F9760-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200810210112.m9L1CX3w004004-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] Re: FW: Identification of gunpowder 2, 28 -- thread-index: AckzGiHoj0bN7OlRSvu1eIDk/d8IuwAWfSmA 2, 28 -- References: {200810210112.m9L1CX3w004004-at-ns.microscopy.com} 2, 28 -- To: {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 21 Oct 2008 12:06:14.0852 (UTC) FILETIME=[6A8C0040:01C93375] 2, 28 -- From: "White, N.W. (Woody)" {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="us-ascii" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9LC6ZT8022108 ==============================End of - Headers==============================
Check with your Geology department or if Clemson's Art department has a jewelry program, with the stone people there. You want the medium and fine polishing compounds used for polishing minerals and gemstones. It would be cheaper to order these from a lapidary supply house, or a hardware store that sells polishing compounds. Get the diamond grit stuff. Any of the histology supply houses sell this also, but usually for higher prices.
Phil
} Email: neerajg-at-clemson.edu } Name: Neeraj } } Organization: Clemson University } } Title-Subject: [Filtered] Microtome Knife Sharpner } } Question: Dear List, } } Our lab has American Optical Model 925 and 935 Microtome knife } sharpners and couple of microtome knives we want to sharpen. We need } the fine and coarse abrasive solution that you need to sharpen the } knives, does anybody know good source for these solution or an } alternative? } } Vendors please feel free to contact me offline! } } Thanks, } } Raj. } } Neeraj V. Gohad, PhD } Postdoctoral Fellow, } Okeanos Research Group } Department of Biological Sciences } 132 Long Hall,Clemson University } Clemson, SC-29634 } 864-656-3597 } neerajg-at-clemson.edu -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 26 -- From oshel1pe-at-cmich.edu Tue Oct 21 08:00:31 2008 4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9LD0VSr006468 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Oct 2008 08:00:31 -0500 4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9LD0Kuf000996; 4, 26 -- Tue, 21 Oct 2008 09:00:23 -0400 4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 26 -- Tue, 21 Oct 2008 08:59:24 -0400 4, 26 -- Mime-Version: 1.0 4, 26 -- Message-Id: {f06240801c52381dc2b43-at-[141.209.160.249]} 4, 26 -- In-Reply-To: {200810211136.m9LBaUPf005772-at-ns.microscopy.com} 4, 26 -- References: {200810211136.m9LBaUPf005772-at-ns.microscopy.com} 4, 26 -- Date: Tue, 21 Oct 2008 08:59:21 -0400 4, 26 -- To: neerajg-at-clemson.edu 4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 26 -- Subject: Re: [Microscopy] viaWWW: Microtome Knife Sharpner 4, 26 -- Cc: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 26 -- X-OriginalArrivalTime: 21 Oct 2008 12:59:24.0541 (UTC) FILETIME=[D7BFEAD0:01C9337C] 4, 26 -- X-Canit-CHI2: 0.00 4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 26 -- X-CanItPRO-Stream: default 4, 26 -- X-Canit-Stats-ID: 4207805 - 48570343ce4c 4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Dear Neeraj, Any Geology or Metallurgy or Materials Engineering department in your university will have diamond polishing compounds in a variety of grit sizes that you can get/borrow/buy. They all do a lot of polishing. Leco, Allied Hi-tech, Struers are some of the vendors that sell polishing supplies. Regards,
-----Original Message----- X-from: neerajg-at-clemson.edu [mailto:neerajg-at-clemson.edu] Sent: October 21, 2008 4:40 AM To: maryflet-at-interchange.ubc.ca
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Our lab has American Optical Model 925 and 935 Microtome knife sharpners and couple of microtome knives we want to sharpen. We need the fine and coarse abrasive solution that you need to sharpen the knives, does anybody know good source for these solution or an alternative?
Vendors please feel free to contact me offline!
Thanks,
Raj.
Neeraj V. Gohad, PhD Postdoctoral Fellow, Okeanos Research Group Department of Biological Sciences 132 Long Hall,Clemson University Clemson, SC-29634 864-656-3597 neerajg-at-clemson.edu
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You order from Thermo Electron, the 6 micron paste called Shandon MicroSharp Diamond Compound 10g/unit. It has 2 Catalog numbers on it so I will give you both. Cat #448533 and Cat# 99920004. This is the course.
The 1 micron Diamond paste has Cat # 448533 (same as above) on it, and also the other Cat # is 99920006, which is different. The phone number on the bottle is 1-800-547-7429.
Hope this helps. I would not go find some other stuff to sharpen with just cause it is cheaper. I would use the tried and true stuff, cause those knives aren't cheap.
Rhonda Trimble HT(ASCP)HTL, QIHC Histology Specialist II Stowers Institute for Medical Research 1000 E 50th Street Kansas City, Missouri 64110 816-926-4346 rra-at-stowers-institute.org
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Email: sarahe-at-smmcape.co.za Name: Sarah Eggleton
Organization: SMM Instruments
Title-Subject: [Filtered] Reichert Jung Polyvar MET
Question: Hi there, I am on the search for a bright field lens for a Reichert Jung Polyvar MET. Anyone have an old unused Polyvar for which they no longer need the bright field lens? Due to the age of the microscope I am unable to source a replacement. As there is nothing else wrong with the microscope, its a waste that we can't use it. Thanks in advance fro any help.. Cheers, Sarah
Dear All, a customer wants to clean pigments (Al, Au, Fe), which are milled to a grain-size of ca. 5 micrometer or smaller. Procedure would be washing with different solutions (water, pet ether).
Problem to be solved: Pigment particles surely tend to stick together during washing and drying, the smaller the particles, the more they stick, I suppose...
On the other hand the customer needs to get as good as possible a separation of the particles to determine the overall area of the particles.
One idea to dry the particles had been to use critical point drying, but I am afraid to contaminate the dryer because I know of no confinement to use in CPD with pores smaller than one micrometer. Spreading the solution out for drying will not be enough because the customer seems to need a certain amount of mass (ca. 1 gramm) for counting...
I would be most grateful for any ideas how to proceed.
When I have to observe fine stone powders in TEM, I resuspend them in Ethanol (methanol), put a drop on grid and slowly blow on it for fast-drying. The result is usually pretty good. If you plan to do it in SEM or light microscopy, you may even heat the support and put the drop on it, it will evaporate almost instantly. Another idea for SEM: filter the suspension, then dry and observe the filter. You should have no problem with the usual 0,2 µm filter. Advantages are that you can wash the particles extensively AND the filter does not contain a metal which may interfere with the analysis of your particles (like alu-holders for SEM). Be careful with the beam though, because the filters are not very resistant to the bem energy. But your particles are :-D
Best regards,
Stephane
----- Original Message ---- X-from: "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} To: nizets2-at-yahoo.com Sent: Thursday, October 23, 2008 12:53:31 PM
Dear All, a customer wants to clean pigments (Al, Au, Fe), which are milled to a grain-size of ca. 5 micrometer or smaller. Procedure would be washing with different solutions (water, pet ether).
Problem to be solved: Pigment particles surely tend to stick together during washing and drying, the smaller the particles, the more they stick, I suppose...
On the other hand the customer needs to get as good as possible a separation of the particles to determine the overall area of the particles.
One idea to dry the particles had been to use critical point drying, but I am afraid to contaminate the dryer because I know of no confinement to use in CPD with pores smaller than one micrometer. Spreading the solution out for drying will not be enough because the customer seems to need a certain amount of mass (ca. 1 gramm) for counting...
I would be most grateful for any ideas how to proceed.
Hello Stefan, I used to prep carbon black samples in a similar manner to Stephane, but I used chloroform. I just them air dry, takes a few minutes and you're ready for TEM analysis. For SEM, I just dust double sticky conductive tape and blow off the extra.
Stay Safe..... Frank Karl.... Linked-in Group Manager
nizets2-at-yahoo.com
10/23/2008 03:45 To PM frank_karl-at-lincolnelectric.com cc
Please respond to Subject nizets2-at-yahoo.com [Microscopy] Re: Cleaning and drying of pigments
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hallo Stefan!
When I have to observe fine stone powders in TEM, I resuspend them in Ethanol (methanol), put a drop on grid and slowly blow on it for fast-drying. The result is usually pretty good. If you plan to do it in SEM or light microscopy, you may even heat the support and put the drop on it, it will evaporate almost instantly. Another idea for SEM: filter the suspension, then dry and observe the filter. You should have no problem with the usual 0,2 µm filter. Advantages are that you can wash the particles extensively AND the filter does not contain a metal which may interfere with the analysis of your particles (like alu-holders for SEM). Be careful with the beam though, because the filters are not very resistant to the bem energy. But your particles are :-D
Best regards,
Stephane
----- Original Message ---- X-from: "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} To: nizets2-at-yahoo.com Sent: Thursday, October 23, 2008 12:53:31 PM
Dear All, a customer wants to clean pigments (Al, Au, Fe), which are milled to a grain-size of ca. 5 micrometer or smaller. Procedure would be washing with different solutions (water, pet ether).
Problem to be solved: Pigment particles surely tend to stick together during washing and drying, the smaller the particles, the more they stick, I suppose...
On the other hand the customer needs to get as good as possible a separation of the particles to determine the overall area of the particles.
One idea to dry the particles had been to use critical point drying, but I am afraid to contaminate the dryer because I know of no confinement to use in CPD with pores smaller than one micrometer. Spreading the solution out for drying will not be enough because the customer seems to need a certain amount of mass (ca. 1 gramm) for counting...
I would be most grateful for any ideas how to proceed.
26, 22 -- Received: from [80.121.18.89] by web37405.mail.mud.yahoo.com via HTTP; Thu, 23 Oct 2008 12:35:33 PDT 26, 22 -- X-Mailer: YahooMailRC/1096.40 YahooMailWebService/0.7.247.3 26, 22 -- Date: Thu, 23 Oct 2008 12:35:33 -0700 (PDT) 26, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 26, 22 -- Subject: Re: [Microscopy] Cleaning and drying of pigments 26, 22 -- To: stefan.diller-at-t-online.de 26, 22 -- Cc: microscopy-at-microscopy.com 26, 22 -- MIME-Version: 1.0 26, 22 -- Content-Type: text/plain; charset=iso-8859-1 26, 22 -- Message-ID: {727336.98515.qm-at-web37405.mail.mud.yahoo.com} 26, 22 -- Content-Transfer-Encoding: 8bit 26, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9NJZXvq021099 ==============================End of - Headers==============================
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==============================Original Headers============================== 3, 24 -- From frank_karl-at-lincolnelectric.com Thu Oct 23 14:54:47 2008 3, 24 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9NJskns002937 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 23 Oct 2008 14:54:46 -0500 3, 24 -- In-Reply-To: {200810231945.m9NJjm6P002286-at-ns.microscopy.com} 3, 24 -- Subject: Re: [Microscopy] Re: Cleaning and drying of pigments 3, 24 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com 3, 24 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 3, 24 -- Message-ID: {OF54526AA8.238C2BD9-ON852574EB.006CB78E-852574EB.006D4CCB-at-lincolnelectric.com} 3, 24 -- Date: Thu, 23 Oct 2008 15:54:37 -0400 3, 24 -- From: Frank_Karl-at-lincolnelectric.com 3, 24 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 3, 24 -- 07, 2008) at 10/23/2008 03:54:29 PM, 3, 24 -- CD-MIME complete at 10/23/2008 03:54:29 PM, 3, 24 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 3, 24 -- 07, 2008) at 10/23/2008 03:54:29 PM, 3, 24 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 3, 24 -- 07, 2008) at 10/23/2008 03:54:29 PM, 3, 24 -- Serialize complete at 10/23/2008 03:54:29 PM 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="ISO-8859-1" 3, 24 -- Content-Transfer-Encoding: 8bit 3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9NJskns002937 ==============================End of - Headers==============================
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, October 23, 2008 3:45 PM To: White, N.W. (Woody)
Hallo Stefan!
When I have to observe fine stone powders in TEM, I resuspend them in Ethanol (methanol), put a drop on grid and slowly blow on it for fast-drying. The result is usually pretty good. If you plan to do it in SEM or light microscopy, you may even heat the support and put the drop on it, it will evaporate almost instantly. Another idea for SEM: filter the suspension, then dry and observe the filter. You should have no problem with the usual 0,2 µm filter. Advantages are that you can wash the particles extensively AND the filter does not contain a metal which may interfere with the analysis of your particles (like alu-holders for SEM). Be careful with the beam though, because the filters are not very resistant to the bem energy. But your particles are :-D
Best regards,
Stephane
----- Original Message ---- X-from: "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} To: nizets2-at-yahoo.com Sent: Thursday, October 23, 2008 12:53:31 PM
Dear All, a customer wants to clean pigments (Al, Au, Fe), which are milled to a grain-size of ca. 5 micrometer or smaller. Procedure would be washing with different solutions (water, pet ether).
Problem to be solved: Pigment particles surely tend to stick together during washing and drying, the smaller the particles, the more they stick, I suppose...
On the other hand the customer needs to get as good as possible a separation of the particles to determine the overall area of the particles.
One idea to dry the particles had been to use critical point drying, but I am afraid to contaminate the dryer because I know of no confinement to use in CPD with pores smaller than one micrometer. Spreading the solution out for drying will not be enough because the customer seems to need a certain amount of mass (ca. 1 gramm) for counting...
I would be most grateful for any ideas how to proceed.
Best regards, Stefan Diller ---------------------------------------------- ----------------------------------------------
My .02 on filtering...
If you consider filtering, and the materials are compatible, check-out Nuclepore Filters (or equal). I really like these filters for SEM work since they are non-fiberous and the filtrate does not get buried inside the filter media. They are not cheap, but worth it to me. A vacuum assisted filtering apparatus should be employed to expedite the job. An example of these filters can be found at: http://www.2spi.com/catalog/spec_prep/filter6.shtml I have no interest in the above, FYI only.
Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Thu Oct 23 15:17:49 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9NKHm0P016739 2, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Oct 2008 15:17:48 -0500 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.11659758; 2, 28 -- Thu, 23 Oct 2008 16:17:30 -0400 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Thu, 23 Oct 2008 16:17:30 -0400 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- Subject: RE: [Microscopy] Re: Cleaning and drying of pigments 2, 28 -- Date: Thu, 23 Oct 2008 16:17:30 -0400 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7033F976D-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200810231944.m9NJiYtn032483-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] Re: Cleaning and drying of pigments 2, 28 -- thread-index: Ack1R8yxWZgRWghCQSqANrNiJBT+jAAAzyLg 2, 28 -- References: {200810231944.m9NJiYtn032483-at-ns.microscopy.com} 2, 28 -- To: {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 23 Oct 2008 20:17:30.0627 (UTC) FILETIME=[604DD530:01C9354C] 2, 28 -- From: "White, N.W. (Woody)" {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="iso-8859-1" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9NKHm0P016739 ==============================End of - Headers==============================
Okay, I need a simple explanation of the way a backscattering detector produces Compositional vs topographical images. I know it is a subtractive process where, if you had a 2-section solid state detector you would have A+B for COMP and A-B for TOPO. But what is actually happening to subtract the elemental information leaving only the topographical information?
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 3, 29 -- From dsherman-at-purdue.edu Fri Oct 24 07:58:09 2008 3, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OCw7Bs018794 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 07:58:08 -0500 3, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 3, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m9OCw5dp012960 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 08:58:05 -0400 3, 29 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 3, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m9OCw27g006479 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 08:58:04 -0400 3, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 3, 29 -- Fri, 24 Oct 2008 08:57:04 -0400 3, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ; 3, 29 -- Fri, 24 Oct 2008 12:56:39 +0000 3, 29 -- User-Agent: Microsoft-Entourage/12.12.0.080729 3, 29 -- Date: Fri, 24 Oct 2008 08:56:38 -0400 3, 29 -- Subject: BSI-topo-comp 3, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 3, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {C5273E46.3540B%dsherman-at-exchange.purdue.edu} 3, 29 -- Thread-Topic: BSI-topo-comp 3, 29 -- Thread-Index: Ack11/O5NfKm1qt8QjeD3MlrTp+42g== 3, 29 -- Mime-version: 1.0 3, 29 -- Content-type: text/plain; 3, 29 -- charset="US-ASCII" 3, 29 -- Content-transfer-encoding: 7bit 3, 29 -- X-OriginalArrivalTime: 24 Oct 2008 12:57:04.0437 (UTC) FILETIME=[037AFE50:01C935D8] 3, 29 -- X-PMX-Version: 5.4.0.320885 3, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
The elemental information isn't really subtracted from the signal. Topographic information results from the line-of-sight nature of backscattered imaging. Since BSEs are not drawn to the detector as SEs are, only those areas of a specimen directly "visible" to the detector are sensed.
If the entire detector surface is used to receive the incoming BSEs, the surface will look more-or-less "evenly illuminated". This is because if BSEs from region A of the sample reach segment 1 of the detector in greater number than they reach segment 2 of the detector, the resulting signal is still the same (or nearly same) strength as if the BSEs from region A reached both segments equally. This is because the output of the segments is read as if they were coming from one detector segment and not 2 (or 4 or ... ). The output signal is summed from the entire detector area.
So, by dividing the detector surface into 2 or 4 (or ... ) segments, the signal from region A reaching segment 1 can be read out separately from the signal from region A reaching segment 2. Subtract the signal of segment 1 from the signal of segment 2, and the difference in signal due to greater or fewer BSEs "seeing" the detector can be determined and presented as a topographic image. A corollary of this is that the topographic image can be changed by dividing the detector into smaller segments (e.g. 4), allowing different pairings of segments. So, if there are segment 1=} 4, then (1+2) - (3+4) will give a different image than (1+3) - (2+4).
The compositional information is still there, it's just lost in the topographic information. Set the detector to read out as a single segment, and the topographic information is now lost and the compositional information can be seen. With the caveat that if there is much topography, the compostional image can be compromised or lost in the signal fluctuations caused by the topography, with with a detector set to "compositional" imaging. So, BSE samples for compositional imaging are generally polished. (Although I do get good compositional imaging from unpolished, low-relief samples.)
Phil P.S. This gives me a chance to plug one of my favorite SEM books: "Scanning Electron Microscopy A Student's Handbook" by Postek, et al., Published & sold by Ladd Research, $39. Yes, it's old and yes it really needs to be updated and I keep hammering at the Ladd people to get on Mike Postek about doing that and encourage everyone else to chime in. Goldstein, et al. is an excellent book, and where I go for the really in-depth stuff, but Postek is the best basic reference and a good teaching book.
} Okay, I need a simple explanation of the way a backscattering detector } produces Compositional vs topographical images. I know it is a subtractive } process where, if you had a 2-section solid state detector you would have } A+B for COMP and A-B for TOPO. But what is actually happening to subtract } the elemental information leaving only the topographical information? } } Debby } -- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.agriculture.purdue.edu/microscopy/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 26 -- From oshel1pe-at-cmich.edu Fri Oct 24 08:48:51 2008 7, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9ODmo8F006447 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 08:48:50 -0500 7, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9ODmn5M005157; 7, 26 -- Fri, 24 Oct 2008 09:48:50 -0400 7, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 26 -- Fri, 24 Oct 2008 09:48:14 -0400 7, 26 -- Mime-Version: 1.0 7, 26 -- Message-Id: {f0624081cc5277b9f03b0-at-[141.209.160.249]} 7, 26 -- In-Reply-To: {200810241304.m9OD4ktL025119-at-ns.microscopy.com} 7, 26 -- References: {200810241304.m9OD4ktL025119-at-ns.microscopy.com} 7, 26 -- Date: Fri, 24 Oct 2008 09:48:11 -0400 7, 26 -- To: dsherman-at-purdue.edu 7, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 26 -- Subject: Re: [Microscopy] BSI-topo-comp 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 26 -- X-OriginalArrivalTime: 24 Oct 2008 13:48:14.0691 (UTC) FILETIME=[297E9330:01C935DF] 7, 26 -- X-Canit-CHI2: 0.00 7, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 26 -- X-Spam-Score: -4.30 () [Hold at 5.00] L_EXCH_MF,L_USDD,RDNS_NONE,Bayes(0.0001,-0.5) 7, 26 -- X-CanItPRO-Stream: default 7, 26 -- X-Canit-Stats-ID: 4375548 - bd1ebdd0ff96 7, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Phil has given a pretty comprehensive explanation of backscattered imaging but one point worth making is the way the backscattered electron detector imaging relates to our eye's view.
Everhart-Thornley detectors receive an image as if the detector itself was lighting the structure i.e. surfaces pointing towards the detector are brighter than those pointing away.
Backscattered electron detectors of the scintillator type "light" the specimen from overhead with a "lighting" bias towards the photomultiplier due to an off balance detection.
Solid state backscattered electron detectors also "light" the image as if from overhead, producing an image with some topography even if you take all of the signal sections. But one big advantage of the detector is that it produces an image similar to the specimen being viewed in a light microscope; also lit from overhead! Those who do not have a multi segment detector (are there any left) may, as in an Everhart-Thornley detector, obtain higher topographic contrast by simply tilting the specimen.
It is interesting that in the more the recent past everyone has been asking about how we measure surface roughness, this too is better viewed in backscatter. Why do people ask this question, well to claim a human face is made smoother by their method. The things we do for science!
One more point, whilst teaching in Australia one of the lecturers commented "We do science, not tradition!" what a superb saying as it seems to me most people go for tradition? I have a similar saying "Microscopists are scientists too, so experiment!"
Its nice to see Debby is clearly experimenting but how many others follow?
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: 24 October 2008 14:00 To: protrain-at-emcourses.com
Okay, I need a simple explanation of the way a backscattering detector produces Compositional vs topographical images. I know it is a subtractive process where, if you had a 2-section solid state detector you would have A+B for COMP and A-B for TOPO. But what is actually happening to A+subtract the elemental information leaving only the topographical information?
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 3, 29 -- From dsherman-at-purdue.edu Fri Oct 24 07:58:09 2008 3, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OCw7Bs018794 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 07:58:08 -0500 3, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 3, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id m9OCw5dp012960 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 08:58:05 -0400 3, 29 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 3, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id m9OCw27g006479 3, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 08:58:04 -0400 3, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 3, 29 -- Fri, 24 Oct 2008 08:57:04 -0400 3, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.10]) with Microsoft Exchange Server HTTP-DAV ; 3, 29 -- Fri, 24 Oct 2008 12:56:39 +0000 3, 29 -- User-Agent: Microsoft-Entourage/12.12.0.080729 3, 29 -- Date: Fri, 24 Oct 2008 08:56:38 -0400 3, 29 -- Subject: BSI-topo-comp 3, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 3, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {C5273E46.3540B%dsherman-at-exchange.purdue.edu} 3, 29 -- Thread-Topic: BSI-topo-comp 3, 29 -- Thread-Index: Ack11/O5NfKm1qt8QjeD3MlrTp+42g== 3, 29 -- Mime-version: 1.0 3, 29 -- Content-type: text/plain; 3, 29 -- charset="US-ASCII" 3, 29 -- Content-transfer-encoding: 7bit 3, 29 -- X-OriginalArrivalTime: 24 Oct 2008 12:57:04.0437 (UTC) FILETIME=[037AFE50:01C935D8] 3, 29 -- X-PMX-Version: 5.4.0.320885 3, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
==============================Original Headers============================== 19, 27 -- From protrain-at-emcourses.com Fri Oct 24 10:11:49 2008 19, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OFBl6g025194 19, 27 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 10:11:48 -0500 19, 27 -- Received: from 5ac8bf4d.bb.sky.com ([90.200.191.77]:3237 helo=HP6220) 19, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 19, 27 -- (envelope-from {protrain-at-emcourses.com} ) 19, 27 -- id 1KtOKH-000JFJ-Ko by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 24 Oct 2008 17:11:46 +0200 19, 27 -- Reply-To: {protrain-at-emcourses.com} 19, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 19, 27 -- To: {dsherman-at-purdue.edu} 19, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 19, 27 -- References: {200810241259.m9OCxYke020335-at-ns.microscopy.com} 19, 27 -- Subject: RE: [Microscopy] BSI-topo-comp 19, 27 -- Date: Fri, 24 Oct 2008 16:11:49 +0100 19, 27 -- Organization: Protrain 19, 27 -- Message-ID: {000801c935ea$d8360c90$0200a8c0-at-HP6220} 19, 27 -- MIME-Version: 1.0 19, 27 -- Content-Type: text/plain; 19, 27 -- charset="us-ascii" 19, 27 -- Content-Transfer-Encoding: 7bit 19, 27 -- X-Mailer: Microsoft Office Outlook 11 19, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 19, 27 -- Thread-Index: Ack12Grumkla+ujQS8CdP0WSE5JktwADz74g 19, 27 -- In-Reply-To: {200810241259.m9OCxYke020335-at-ns.microscopy.com} 19, 27 -- X-Scan-Signature: 894d90fafaaebf91f0e06465a8a56d3f{115}} 19, 27 -- X-Trace: smtp01.dial-up.net 1KtOKH-000JFJ-Ko 044a65dd7683aa01c676f1084c60cc1d ==============================End of - Headers==============================
So I get this email followed by a phone call regarding glass plates. I have some developed ones but I am not old timer enough to have had to use them. My response to this student was that I would contact the microscopy group, but not to hold out hope for a case of unused glass negatives. I also suggested making them herself because there are recipes out there because this was a lab exercise at one time. So, any suggestions or recipes? Don't astronomers use glass plates too?
jg
Leslie Koptcho, lkoptcho wrote: } Greetings, } I am writing to see if anyone can help me with locating Kodak (glass) } projector slide plates 3 1/2” x 4” --- Kodak cat # 140 6909. I have } tried Kodak and other photo related vendors, but with no luck. I was } wondering if there might be some of these “vintage” plates and/or } projectors somewhere within your department, within a colleagues } department or a lead to find the plates or similar product. I would } appreciate any assistance you might be able to provide. } Leslie Koptcho } Louisiana State University
==============================Original Headers============================== 6, 20 -- From grazul-at-ccmr.cornell.edu Fri Oct 24 10:12:56 2008 6, 20 -- Received: from mercury.ccmr.cornell.edu (mercury.ccmr.cornell.edu [128.84.231.97]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OFCsQv026735 6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 10:12:56 -0500 6, 20 -- Received: from [128.253.63.133] (bob2.ccmr.cornell.edu [128.253.63.133]) 6, 20 -- by mercury.ccmr.cornell.edu (8.13.6/8.13.6) with ESMTP id m9OFCs2F023997 6, 20 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 11:12:54 -0400 6, 20 -- Message-ID: {4901E5F4.5080500-at-ccmr.cornell.edu} 6, 20 -- Date: Fri, 24 Oct 2008 11:12:52 -0400 6, 20 -- From: John Grazul {grazul-at-ccmr.cornell.edu} 6, 20 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: microscopy-at-microscopy.com 6, 20 -- Subject: Query glass slide plates..can you help this poor soul? 6, 20 -- References: {C526595A.2768%lkoptcho-at-lsu.edu} 6, 20 -- In-Reply-To: {C526595A.2768%lkoptcho-at-lsu.edu} 6, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-Virus-Scanned: ClamAV 0.93.1/8485/Fri Oct 24 09:38:51 2008 on mercury.ccmr.cornell.edu 6, 20 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I take the oportunity to ask a question more about two sectors BSE detector imaging.
As far I understand the way it works, the topo (A-B or B-A) image is not a true rendering of the topography of the sample, but more the derivative of the topography. If one look at a sample with flat steps, only the border of the steps will apear as a dark or bright contrast. The steps themself will be all more or less the same gray, flattening the topographical aspect of the surface. One don't see the topography, but more it variations.
Since more then twenty years I work on SEM's, I cannot remember a sample/situation where this "topo" image was THE good way to answer a question (in contrast with the compo/A+B mode, whose interest is very evident).
So, I would be interested in some exemples where this type of images brings something more than the Everhart-Thornley detector.
Tanks and have a good WE (it's 5:30 PM here !). I'll find the answers on monday !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
oshel1pe-at-cmich.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Debby, } } The elemental information isn't really subtracted from the signal. } Topographic information results from the line-of-sight nature of } backscattered imaging. Since BSEs are not drawn to the detector as } SEs are, only those areas of a specimen directly "visible" to the } detector are sensed. } } If the entire detector surface is used to receive the incoming BSEs, } the surface will look more-or-less "evenly illuminated". This is } because if BSEs from region A of the sample reach segment 1 of the } detector in greater number than they reach segment 2 of the detector, } the resulting signal is still the same (or nearly same) strength as } if the BSEs from region A reached both segments equally. This is } because the output of the segments is read as if they were coming } from one detector segment and not 2 (or 4 or ... ). The output signal } is summed from the entire detector area. } } So, by dividing the detector surface into 2 or 4 (or ... ) segments, } the signal from region A reaching segment 1 can be read out } separately from the signal from region A reaching segment 2. Subtract } the signal of segment 1 from the signal of segment 2, and the } difference in signal due to greater or fewer BSEs "seeing" the } detector can be determined and presented as a topographic image. } A corollary of this is that the topographic image can be changed by } dividing the detector into smaller segments (e.g. 4), allowing } different pairings of segments. So, if there are segment 1=} 4, then } (1+2) - (3+4) will give a different image than (1+3) - (2+4). } } The compositional information is still there, it's just lost in the } topographic information. Set the detector to read out as a single } segment, and the topographic information is now lost and the } compositional information can be seen. } With the caveat that if there is much topography, the compostional } image can be compromised or lost in the signal fluctuations caused by } the topography, with with a detector set to "compositional" imaging. } So, BSE samples for compositional imaging are generally polished. } (Although I do get good compositional imaging from unpolished, } low-relief samples.) } } Phil } P.S. This gives me a chance to plug one of my favorite SEM books: } "Scanning Electron Microscopy A Student's Handbook" by Postek, et } al., Published & sold by Ladd Research, $39. Yes, it's old and yes } it really needs to be updated and I keep hammering at the Ladd people } to get on Mike Postek about doing that and encourage everyone else to } chime in. Goldstein, et al. is an excellent book, and where I go for } the really in-depth stuff, but Postek is the best basic reference and } a good teaching book. } } } } Okay, I need a simple explanation of the way a backscattering detector } } produces Compositional vs topographical images. I know it is a subtractive } } process where, if you had a 2-section solid state detector you would have } } A+B for COMP and A-B for TOPO. But what is actually happening to subtract } } the elemental information leaving only the topographical information? } } } } Debby } } -- } } Debby Sherman, Director Phone: 765-494-6666 } } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: dsherman-at-purdue.edu } } S-052 Whistler Building } } 170 S. University Street } } West Lafayette, IN 47907 } } http://www.agriculture.purdue.edu/microscopy/ } }
==============================Original Headers============================== 11, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Oct 24 10:49:24 2008 11, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OFnMpZ021438 11, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 10:49:23 -0500 11, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 11, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m9OFnKLG066784 11, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 17:49:20 +0200 (CEST) 11, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 13BBF40403C 11, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Oct 2008 17:46:52 +0200 (CEST) 11, 29 -- Message-ID: {4901EDEC.508-at-ipcms.u-strasbg.fr} 11, 29 -- Date: Fri, 24 Oct 2008 17:46:52 +0200 11, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 29 -- User-Agent: Thunderbird 2.0.0.17 (X11/20080925) 11, 29 -- MIME-Version: 1.0 11, 29 -- To: Microscopy-at-microscopy.com 11, 29 -- Subject: Re: BSI-topo-comp : a quest. more 11, 29 -- References: {200810241352.m9ODqdMU014363-at-ns.microscopy.com} 11, 29 -- In-Reply-To: {200810241352.m9ODqdMU014363-at-ns.microscopy.com} 11, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-IPCMS-MailScanner: Found to be clean 11, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 11, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.154]); Fri, 24 Oct 2008 17:49:20 +0200 (CEST) 11, 29 -- X-Virus-Scanned: ClamAV 0.94/8485/Fri Oct 24 15:38:51 2008 on mr4.u-strasbg.fr 11, 29 -- X-Virus-Status: Clean 11, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=disabled 11, 29 -- version=3.2.5 11, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr ==============================End of - Headers==============================
BSE signals are the result of the intensity due to composition modified by the geometry of the location. For small tilts around horizontal, you might say that the overall intensity pattern of the BSE emission is the same, but it is shifted off center toward one portion or another of the detector. Thus the various segments would no longer give identical signals. The difference would be due to topography.
So is a TOPO-mode signal void of composition information? I doubt it. I would expect the fluctuation in signal due to topography in copper to be much larger than the fluctuation due to the same topography in silicon. Proportionally, the changes in signal may be similar, but the absolute changes in signal would be less for Si than for Cu. If similar topographies were present for both phases in the same image, I would expect the Cu side to show more contrast than the Si side. The average brightness should be the same for both sides.
So how is it that a COMP-mode signal still shows topography? You might say that the angles are no longer small and the BSE pattern has not simply shifted from one segment of the detector to another, but has actually moved off the detector so that overall intensity decreases. Also, the intensity of the entire BSE signal may be altered by the change in geometry. Now, the topographic information may not be very evident or easy to interpret in COMP mode. It is like a coaxial lighting arrangement. I can tell that my intensity is down, but I cannot tell which way it scattered. To do that, I need to invoke some measure or other of topographic mode. I may not need to go all the way to A-B, maybe just A would be enough. Now our older JEOL does not allow total freedom of selecting the quadrants, but it does allow us to combine signals. Thus, COMP+TOPO = (A+B)+(A-B) = 2A.
I hope this helps. Warren Straszheim Iowa State University
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Friday, October 24, 2008 8:50 AM To: wesaia-at-iastate.edu
Hi Jacques
Well we are both on the same wave length here! In my 40+ years with SEM I too have not seen a "topographic" imaged, gained by subtracting signals, that solved a problem and I am working with people's problems almost all of the time.
In many consultancy tasks, relating to failure analysis, we are very often using the BSE image in its so called compo mode to solve problems, as the best information about a failure is not always on the surface. We are also using BSE detection, as I said earlier, because it reduces the high contrast differences sometimes dominant in rough fractures; even at what I would call the correct kV! This also ties together the light microscopy with the SEM.
Hope this helps?
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: 24 October 2008 16:51 To: protrain-at-emcourses.com
Hi
I take the oportunity to ask a question more about two sectors BSE detector imaging.
As far I understand the way it works, the topo (A-B or B-A) image is not a true rendering of the topography of the sample, but more the derivative of the topography. If one look at a sample with flat steps, only the border of the steps will apear as a dark or bright contrast. The steps themself will be all more or less the same gray, flattening the topographical aspect of the surface. One don't see the topography, but more it variations.
Since more then twenty years I work on SEM's, I cannot remember a sample/situation where this "topo" image was THE good way to answer a question (in contrast with the compo/A+B mode, whose interest is very evident).
So, I would be interested in some exemples where this type of images brings something more than the Everhart-Thornley detector.
Tanks and have a good WE (it's 5:30 PM here !). I'll find the answers on monday !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
oshel1pe-at-cmich.edu a écrit : } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Debby, } } The elemental information isn't really subtracted from the signal. } Topographic information results from the line-of-sight nature of } backscattered imaging. Since BSEs are not drawn to the detector as SEs } are, only those areas of a specimen directly "visible" to the detector } are sensed. } } If the entire detector surface is used to receive the incoming BSEs, } the surface will look more-or-less "evenly illuminated". This is } because if BSEs from region A of the sample reach segment 1 of the } detector in greater number than they reach segment 2 of the detector, } the resulting signal is still the same (or nearly same) strength as if } the BSEs from region A reached both segments equally. This is because } the output of the segments is read as if they were coming from one } detector segment and not 2 (or 4 or ... ). The output signal is summed } from the entire detector area. } } So, by dividing the detector surface into 2 or 4 (or ... ) segments, } the signal from region A reaching segment 1 can be read out separately } from the signal from region A reaching segment 2. Subtract the signal } of segment 1 from the signal of segment 2, and the difference in } signal due to greater or fewer BSEs "seeing" the detector can be } determined and presented as a topographic image. } A corollary of this is that the topographic image can be changed by } dividing the detector into smaller segments (e.g. 4), allowing } different pairings of segments. So, if there are segment 1=} 4, then } (1+2) - (3+4) will give a different image than (1+3) - (2+4). } } The compositional information is still there, it's just lost in the } topographic information. Set the detector to read out as a single } segment, and the topographic information is now lost and the } compositional information can be seen. } With the caveat that if there is much topography, the compostional } image can be compromised or lost in the signal fluctuations caused by } the topography, with with a detector set to "compositional" imaging. } So, BSE samples for compositional imaging are generally polished. } (Although I do get good compositional imaging from unpolished, } low-relief samples.) } } Phil } P.S. This gives me a chance to plug one of my favorite SEM books: } "Scanning Electron Microscopy A Student's Handbook" by Postek, et al., } Published & sold by Ladd Research, $39. Yes, it's old and yes it } really needs to be updated and I keep hammering at the Ladd people to } get on Mike Postek about doing that and encourage everyone else to } chime in. Goldstein, et al. is an excellent book, and where I go for } the really in-depth stuff, but Postek is the best basic reference and } a good teaching book. } } } } Okay, I need a simple explanation of the way a backscattering } } detector produces Compositional vs topographical images. I know it } } is a subtractive process where, if you had a 2-section solid state } } detector you would have } } A+B for COMP and A-B for TOPO. But what is actually happening to } } A+subtract } } the elemental information leaving only the topographical information? } } } } Debby } } -- } } Debby Sherman, Director Phone: 765-494-6666 } } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: dsherman-at-purdue.edu } } S-052 Whistler Building } } 170 S. University Street } } West Lafayette, IN 47907 } } http://www.agriculture.purdue.edu/microscopy/ } }
==============================Original Headers============================== 11, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Oct 24 10:49:24 2008 11, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.154]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OFnMpZ021438 11, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 10:49:23 -0500 11, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 11, 29 -- by mailhost.u-strasbg.fr (8.14.2/jtpda-5.5pre1) with ESMTP id m9OFnKLG066784 11, 29 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 17:49:20 +0200 (CEST) 11, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 11, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 13BBF40403C 11, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Oct 2008 17:46:52 +0200 (CEST) 11, 29 -- Message-ID: {4901EDEC.508-at-ipcms.u-strasbg.fr} 11, 29 -- Date: Fri, 24 Oct 2008 17:46:52 +0200 11, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 11, 29 -- User-Agent: Thunderbird 2.0.0.17 (X11/20080925) 11, 29 -- MIME-Version: 1.0 11, 29 -- To: Microscopy-at-microscopy.com 11, 29 -- Subject: Re: BSI-topo-comp : a quest. more 11, 29 -- References: {200810241352.m9ODqdMU014363-at-ns.microscopy.com} 11, 29 -- In-Reply-To: {200810241352.m9ODqdMU014363-at-ns.microscopy.com} 11, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-IPCMS-MailScanner: Found to be clean 11, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 11, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.0 (mailhost.u-strasbg.fr [130.79.200.154]); Fri, 24 Oct 2008 17:49:20 +0200 (CEST) 11, 29 -- X-Virus-Scanned: ClamAV 0.94/8485/Fri Oct 24 15:38:51 2008 on mr4.u-strasbg.fr 11, 29 -- X-Virus-Status: Clean 11, 29 -- X-Spam-Status: No, score=0.0 required=5.0 tests=none autolearn=disabled 11, 29 -- version=3.2.5 11, 29 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on mr4.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 23, 28 -- From protrain-at-emcourses.com Fri Oct 24 11:36:22 2008 23, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 23, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OGaKFI011186 23, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 11:36:21 -0500 23, 28 -- Received: from 5ac8bf4d.bb.sky.com ([90.200.191.77]:4115 helo=HP6220) 23, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 23, 28 -- (envelope-from {protrain-at-emcourses.com} ) 23, 28 -- id 1KtPe7-000LSf-0h by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 24 Oct 2008 18:36:19 +0200 23, 28 -- Reply-To: {protrain-at-emcourses.com} 23, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 23, 28 -- To: {jacques.faerber-at-ipcms.u-strasbg.fr} 23, 28 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 23, 28 -- References: {200810241550.m9OFooIM022961-at-ns.microscopy.com} 23, 28 -- Subject: RE: [Microscopy] Re: BSI-topo-comp : a quest. more 23, 28 -- Date: Fri, 24 Oct 2008 17:36:01 +0100 23, 28 -- Organization: Protrain 23, 28 -- Message-ID: {005f01c935f6$a82da380$0200a8c0-at-HP6220} 23, 28 -- MIME-Version: 1.0 23, 28 -- Content-Type: text/plain; 23, 28 -- charset="iso-8859-1" 23, 28 -- X-Mailer: Microsoft Office Outlook 11 23, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 23, 28 -- Thread-Index: Ack18FdPCc1rGmHbSe2a5OLsZDmvUwAAxmgg 23, 28 -- In-Reply-To: {200810241550.m9OFooIM022961-at-ns.microscopy.com} 23, 28 -- X-Scan-Signature: 67cad4ebc52b7006c6a4458ffc8be92f{238}} 23, 28 -- X-Trace: smtp01.dial-up.net 1KtPe7-000LSf-0h ce1ede4b0ca09fa7938eec077ae9e02a 23, 28 -- Content-Transfer-Encoding: 8bit 23, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OGaKFI011186 ==============================End of - Headers==============================
I too will be interested in the answers. The only advantages I know of for using topographic BSE imaging are: 1) It can be a good way around charging problems. The imaged BSEs have nearly the same energy as the beam electrons, so they will be less affected by specimen charging. In the Old Days before environmental and variable-vacuum SEMs and gas in the chamber to bleed off charge, BSE was the only hope for imaging chargey samples that could not be coated (like museum specimens). 2) TOPO imaging can be used to get a "sort of SE-like" image of a specimen on which BSE imaging or E/WDS is being done. Usually it's difficult to get SE images of such subjects, since the specimens are either uncoated or carbon-coated and so chargey, and the BSE topographic image can be collected without changing any of the operating parameters -- spot size, aperture, working distance, etc. and so on. Just flip the detector switches from COMPO to TOPO and maybe rebalance the contrast and brightness.
Otherwise, I'm in your camp, SE imaging is better. Mind, it's really cool when you can do both at once, which can be done with the right BSE detector (like an Autrata).
Phil
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-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 28 -- From oshel1pe-at-cmich.edu Fri Oct 24 11:47:39 2008 7, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OGldua000994 7, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 11:47:39 -0500 7, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 28 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OGlYTn015285; 7, 28 -- Fri, 24 Oct 2008 12:47:34 -0400 7, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 28 -- Fri, 24 Oct 2008 12:47:29 -0400 7, 28 -- Mime-Version: 1.0 7, 28 -- Message-Id: {f06240827c527a7ad56b6-at-[141.209.160.249]} 7, 28 -- In-Reply-To: {200810241553.m9OFrwvh027769-at-ns.microscopy.com} 7, 28 -- References: {200810241553.m9OFrwvh027769-at-ns.microscopy.com} 7, 28 -- Date: Fri, 24 Oct 2008 12:47:27 -0400 7, 28 -- To: jacques.faerber-at-ipcms.u-strasbg.fr 7, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 28 -- Subject: [Microscopy] Re: BSI-topo-comp : a quest. more 7, 28 -- Cc: Microscopy-at-microscopy.com 7, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 28 -- X-OriginalArrivalTime: 24 Oct 2008 16:47:29.0968 (UTC) FILETIME=[34239B00:01C935F8] 7, 28 -- X-Canit-CHI2: 0.00 7, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 28 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 28 -- X-CanItPRO-Stream: default 7, 28 -- X-Canit-Stats-ID: 4384972 - 88640de707d5 7, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OGldua000994 ==============================End of - Headers==============================
I do not agree that SE images are better but I am sure you would modify that to say there is a technique for a problem?
Most modern systems offer dual display so we can all enjoy comparing the Everhart-Thornley image with the BSE image without complicated image mixing boxes; progress?
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: 24 October 2008 17:48 To: protrain-at-emcourses.com
Jacques,
I too will be interested in the answers. The only advantages I know of for using topographic BSE imaging are: 1) It can be a good way around charging problems. The imaged BSEs have nearly the same energy as the beam electrons, so they will be less affected by specimen charging. In the Old Days before environmental and variable-vacuum SEMs and gas in the chamber to bleed off charge, BSE was the only hope for imaging chargey samples that could not be coated (like museum specimens). 2) TOPO imaging can be used to get a "sort of SE-like" image of a specimen on which BSE imaging or E/WDS is being done. Usually it's difficult to get SE images of such subjects, since the specimens are either uncoated or carbon-coated and so chargey, and the BSE topographic image can be collected without changing any of the operating parameters -- spot size, aperture, working distance, etc. and so on. Just flip the detector switches from COMPO to TOPO and maybe rebalance the contrast and brightness.
Otherwise, I'm in your camp, SE imaging is better. Mind, it's really cool when you can do both at once, which can be done with the right BSE detector (like an Autrata).
Phil
} ----------------------------------------------------------------------- } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 28 -- From oshel1pe-at-cmich.edu Fri Oct 24 11:47:39 2008 7, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OGldua000994 7, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 11:47:39 -0500 7, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 28 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OGlYTn015285; 7, 28 -- Fri, 24 Oct 2008 12:47:34 -0400 7, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 28 -- Fri, 24 Oct 2008 12:47:29 -0400 7, 28 -- Mime-Version: 1.0 7, 28 -- Message-Id: {f06240827c527a7ad56b6-at-[141.209.160.249]} 7, 28 -- In-Reply-To: {200810241553.m9OFrwvh027769-at-ns.microscopy.com} 7, 28 -- References: {200810241553.m9OFrwvh027769-at-ns.microscopy.com} 7, 28 -- Date: Fri, 24 Oct 2008 12:47:27 -0400 7, 28 -- To: jacques.faerber-at-ipcms.u-strasbg.fr 7, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 28 -- Subject: [Microscopy] Re: BSI-topo-comp : a quest. more 7, 28 -- Cc: Microscopy-at-microscopy.com 7, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 28 -- X-OriginalArrivalTime: 24 Oct 2008 16:47:29.0968 (UTC) FILETIME=[34239B00:01C935F8] 7, 28 -- X-Canit-CHI2: 0.00 7, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 28 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 28 -- X-CanItPRO-Stream: default 7, 28 -- X-Canit-Stats-ID: 4384972 - 88640de707d5 7, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OGldua000994 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From protrain-at-emcourses.com Fri Oct 24 12:03:04 2008 19, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OH32Yc015256 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 12:03:03 -0500 19, 28 -- Received: from 5ac8bf4d.bb.sky.com ([90.200.191.77]:1853 helo=HP6220) 19, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 19, 28 -- (envelope-from {protrain-at-emcourses.com} ) 19, 28 -- id 1KtQ3u-000M21-C3 by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 24 Oct 2008 19:02:59 +0200 19, 28 -- Reply-To: {protrain-at-emcourses.com} 19, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 19, 28 -- To: {oshel1pe-at-cmich.edu} 19, 28 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 19, 28 -- References: {200810241648.m9OGmRsM002475-at-ns.microscopy.com} 19, 28 -- Subject: RE: [Microscopy] Re: BSI-topo-comp : a quest. more 19, 28 -- Date: Fri, 24 Oct 2008 18:03:02 +0100 19, 28 -- Organization: Protrain 19, 28 -- Message-ID: {006401c935fa$61762a80$0200a8c0-at-HP6220} 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="iso-8859-1" 19, 28 -- X-Mailer: Microsoft Office Outlook 11 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 19, 28 -- Thread-Index: Ack1+GKmqta7P6lfQ+iDRaACWBgcEgAAZJFw 19, 28 -- In-Reply-To: {200810241648.m9OGmRsM002475-at-ns.microscopy.com} 19, 28 -- X-Scan-Signature: 835db587ac8434bdf5b3b6b27df527e1{263}} 19, 28 -- X-Trace: smtp01.dial-up.net 1KtQ3u-000M21-C3 52ad158ca9be8f64f648f925d2c06db8 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OH32Yc015256 ==============================End of - Headers==============================
I'm not sure I follow you. I thought I was agreeing with you. I do think SE gives higher resolution, and resolution being the sine qua non of microscopy, tney are "better". See the SEMs on: http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Programs/microscopy/gallery.html These were taken with an Autrata BSE, which produces near-SE quality images at 5kV. Or even 3kV. Good as they are, the SEs are still better. Keep in mind, the BSEs are also coming from a larger analyzed volume, and so have lower spatial resolution that do SEs. Even with the best BSE detector.
There is indeed a technique for a problem. That's essentially my point with using BSEs for imaging chargey samples, or for TOPO imaging of samples primarill used for COMPO BSE or x-ray.
As far as I know *all* SEMs offer dual display. X-from the JEOL 35 I learned on mumblety years ago to now. Only SEM I've ever used that didn't have dual display was a Cambridge Mk IIa.
Phil
} Hi Phil } } I do not agree that SE images are better but I am sure you would modify that } to say there is a technique for a problem? } } Most modern systems offer dual display so we can all enjoy comparing the } Everhart-Thornley image with the BSE image without complicated image mixing } boxes; progress? } } } Steve Chapman } Protrain } For training and consultancy in electron microscopy world wide } Tel +44 1280 816512 Fax +44 1280 814007 } Cell +44 7711 606967 www.emcourses.com } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: 24 October 2008 17:48 } To: protrain-at-emcourses.com } Subject: [Microscopy] Re: BSI-topo-comp : a quest. more } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 27 -- From oshel1pe-at-cmich.edu Fri Oct 24 12:38:26 2008 7, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OHcP4w030466 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 12:38:25 -0500 7, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OHcHB0003544 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:38:21 -0400 7, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 24 Oct 2008 13:38:19 -0400 7, 27 -- Mime-Version: 1.0 7, 27 -- Message-Id: {f0624082ac527b86d43e0-at-[141.209.160.249]} 7, 27 -- In-Reply-To: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- References: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- Date: Fri, 24 Oct 2008 13:38:16 -0400 7, 27 -- To: Microscopy-at-microscopy.com 7, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 27 -- Subject: [Microscopy] RE: Re: BSI-topo-comp : a quest. more 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 27 -- X-OriginalArrivalTime: 24 Oct 2008 17:38:19.0211 (UTC) FILETIME=[4DA129B0:01C935FF] 7, 27 -- X-Canit-CHI2: 0.00 7, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 27 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 27 -- X-CanItPRO-Stream: default 7, 27 -- X-Canit-Stats-ID: 4387496 - 6ceb9297fe71 7, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OHcP4w030466 ==============================End of - Headers==============================
Dear Phil, One other advantage of the "topo" BSE is that the new "table Top" SEMs are fixed variable-pressure, BSE imaging only, so the "topo" option may get a better image of the surface topography than the "compo" BSE image. Regards,
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: October 24, 2008 10:45 AM To: maryflet-at-interchange.ubc.ca
Steve,
I'm not sure I follow you. I thought I was agreeing with you. I do think SE gives higher resolution, and resolution being the sine qua non of microscopy, tney are "better". See the SEMs on: http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Progr ams/microscopy/gallery.html These were taken with an Autrata BSE, which produces near-SE quality images at 5kV. Or even 3kV. Good as they are, the SEs are still better. Keep in mind, the BSEs are also coming from a larger analyzed volume, and so have lower spatial resolution that do SEs. Even with the best BSE detector.
There is indeed a technique for a problem. That's essentially my point with using BSEs for imaging chargey samples, or for TOPO imaging of samples primarill used for COMPO BSE or x-ray.
As far as I know *all* SEMs offer dual display. X-from the JEOL 35 I learned on mumblety years ago to now. Only SEM I've ever used that didn't have dual display was a Cambridge Mk IIa.
Phil
} Hi Phil } } I do not agree that SE images are better but I am sure you would modify that } to say there is a technique for a problem? } } Most modern systems offer dual display so we can all enjoy comparing the } Everhart-Thornley image with the BSE image without complicated image mixing } boxes; progress? } } } Steve Chapman } Protrain } For training and consultancy in electron microscopy world wide } Tel +44 1280 816512 Fax +44 1280 814007 } Cell +44 7711 606967 www.emcourses.com } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: 24 October 2008 17:48 } To: protrain-at-emcourses.com } Subject: [Microscopy] Re: BSI-topo-comp : a quest. more } } } } } --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 27 -- From oshel1pe-at-cmich.edu Fri Oct 24 12:38:26 2008 7, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OHcP4w030466 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 12:38:25 -0500 7, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OHcHB0003544 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:38:21 -0400 7, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 24 Oct 2008 13:38:19 -0400 7, 27 -- Mime-Version: 1.0 7, 27 -- Message-Id: {f0624082ac527b86d43e0-at-[141.209.160.249]} 7, 27 -- In-Reply-To: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- References: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- Date: Fri, 24 Oct 2008 13:38:16 -0400 7, 27 -- To: Microscopy-at-microscopy.com 7, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 27 -- Subject: [Microscopy] RE: Re: BSI-topo-comp : a quest. more 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 27 -- X-OriginalArrivalTime: 24 Oct 2008 17:38:19.0211 (UTC) FILETIME=[4DA129B0:01C935FF] 7, 27 -- X-Canit-CHI2: 0.00 7, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 27 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 27 -- X-CanItPRO-Stream: default 7, 27 -- X-Canit-Stats-ID: 4387496 - 6ceb9297fe71 7, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OHcP4w030466 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 32 -- From maryflet-at-interchange.ubc.ca Fri Oct 24 13:12:12 2008 16, 32 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) 16, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OICBOP013364 16, 32 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:12:12 -0500 16, 32 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 16, 32 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP id 890C81EEB5 16, 32 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 11:12:09 -0700 (PDT) 16, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 16, 32 -- by smtp.interchange.ubc.ca 16, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 16, 32 -- with ESMTP id {0K9900GFZ9889Q-at-smtp.interchange.ubc.ca} for 16, 32 -- microscopy-at-microscopy.com; Fri, 24 Oct 2008 11:12:09 -0700 (PDT) 16, 32 -- Date: Fri, 24 Oct 2008 11:12:15 -0700 16, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 16, 32 -- Subject: RE: [Microscopy] BSI-topo-comp : a quest. more 16, 32 -- In-reply-to: {200810241744.m9OHibcb007813-at-ns.microscopy.com} 16, 32 -- To: oshel1pe-at-cmich.edu 16, 32 -- Cc: microscopy-at-microscopy.com 16, 32 -- Reply-to: maryflet-at-interchange.ubc.ca 16, 32 -- Message-id: {0K9900GG09889Q-at-smtp.interchange.ubc.ca} 16, 32 -- Organization: Materials Eng. 16, 32 -- MIME-version: 1.0 16, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 16, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 32 -- Content-type: text/plain; charset=us-ascii 16, 32 -- Content-transfer-encoding: 7bit 16, 32 -- Thread-index: Ack2ADAAljEjYkrITKiIjqLn8zjOxQAA428w 16, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.10.24.175224 16, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 16, 32 -- X-PerlMx-Spam: Probability=8%, Report=BODY_SIZE_10000_PLUS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 16, 32 -- X-Spam-Level: 16, 32 -- X-Spam-Flag: No ==============================End of - Headers==============================
Yes I too use 5kV BSE with whatever the client has as a detector, but I think my real point is resolution or information?
On many courses the clients think that they want higher resolution but 7 times out of 10 they are really talking about information. I believe, in many of the cases that I investigate on behalf of clients, it is actually the combination of Everhart-Thornley data and BSE data that finally solves the problem. My personal belief is that you should look at any sample with as many views as possible, SE, BSE and kV variations all of which build towards the final understanding of the specimen. Even doing crazy things like "it still charges at 2kV" and going up to 15kV in BSE often brings success.
With Field Emission the problem of resolution is fading away and searching for the best presentation of the critical information is becoming the main goal.
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: 24 October 2008 18:40 To: protrain-at-emcourses.com
Steve,
I'm not sure I follow you. I thought I was agreeing with you. I do think SE gives higher resolution, and resolution being the sine qua non of microscopy, tney are "better". See the SEMs on: http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Progr ams/microscopy/gallery.html These were taken with an Autrata BSE, which produces near-SE quality images at 5kV. Or even 3kV. Good as they are, the SEs are still better. Keep in mind, the BSEs are also coming from a larger analyzed volume, and so have lower spatial resolution that do SEs. Even with the best BSE detector.
There is indeed a technique for a problem. That's essentially my point with using BSEs for imaging chargey samples, or for TOPO imaging of samples primarill used for COMPO BSE or x-ray.
As far as I know *all* SEMs offer dual display. X-from the JEOL 35 I learned on mumblety years ago to now. Only SEM I've ever used that didn't have dual display was a Cambridge Mk IIa.
Phil
} Hi Phil } } I do not agree that SE images are better but I am sure you would modify } that to say there is a technique for a problem? } } Most modern systems offer dual display so we can all enjoy comparing } the Everhart-Thornley image with the BSE image without complicated } image mixing boxes; progress? } } } Steve Chapman } Protrain } For training and consultancy in electron microscopy world wide Tel +44 } 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: 24 October 2008 17:48 } To: protrain-at-emcourses.com } Subject: [Microscopy] Re: BSI-topo-comp : a quest. more } } } } } ----------------------------------------------------------------------- } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
==============================Original Headers============================== 7, 27 -- From oshel1pe-at-cmich.edu Fri Oct 24 12:38:26 2008 7, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OHcP4w030466 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 12:38:25 -0500 7, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OHcHB0003544 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:38:21 -0400 7, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 24 Oct 2008 13:38:19 -0400 7, 27 -- Mime-Version: 1.0 7, 27 -- Message-Id: {f0624082ac527b86d43e0-at-[141.209.160.249]} 7, 27 -- In-Reply-To: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- References: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- Date: Fri, 24 Oct 2008 13:38:16 -0400 7, 27 -- To: Microscopy-at-microscopy.com 7, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 27 -- Subject: [Microscopy] RE: Re: BSI-topo-comp : a quest. more 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 27 -- X-OriginalArrivalTime: 24 Oct 2008 17:38:19.0211 (UTC) FILETIME=[4DA129B0:01C935FF] 7, 27 -- X-Canit-CHI2: 0.00 7, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 27 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 27 -- X-CanItPRO-Stream: default 7, 27 -- X-Canit-Stats-ID: 4387496 - 6ceb9297fe71 7, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OHcP4w030466 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 27 -- From protrain-at-emcourses.com Fri Oct 24 13:14:17 2008 19, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OIEGFd017130 19, 27 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:14:17 -0500 19, 27 -- Received: from 5ac8bf4d.bb.sky.com ([90.200.191.77]:2800 helo=HP6220) 19, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 19, 27 -- (envelope-from {protrain-at-emcourses.com} ) 19, 27 -- id 1KtRAr-000NeA-I3 by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 24 Oct 2008 20:14:14 +0200 19, 27 -- Reply-To: {protrain-at-emcourses.com} 19, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 19, 27 -- To: {oshel1pe-at-cmich.edu} 19, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 19, 27 -- References: {200810241739.m9OHdbIf031955-at-ns.microscopy.com} 19, 27 -- Subject: RE: [Microscopy] BSI-topo-comp : a quest. more 19, 27 -- Date: Fri, 24 Oct 2008 19:14:17 +0100 19, 27 -- Organization: Protrain 19, 27 -- Message-ID: {006b01c93604$55a97db0$0200a8c0-at-HP6220} 19, 27 -- MIME-Version: 1.0 19, 27 -- Content-Type: text/plain; 19, 27 -- charset="US-ASCII" 19, 27 -- Content-Transfer-Encoding: 7bit 19, 27 -- X-Mailer: Microsoft Office Outlook 11 19, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 19, 27 -- Thread-Index: Ack1/4iDVDGnBUtKRmmTT0d7mH6PrQAAqXMA 19, 27 -- In-Reply-To: {200810241739.m9OHdbIf031955-at-ns.microscopy.com} 19, 27 -- X-Scan-Signature: 69743494267edaeebc707614cd36e3e7{307}} 19, 27 -- X-Trace: smtp01.dial-up.net 1KtRAr-000NeA-I3 460a0de4a04b92ce3c9e0617918c3a81 ==============================End of - Headers==============================
Don't you think the topo option is too dramatic, I never like to throw away electrons? Would it be better to use a little tilt and control the loss of signal to enhance topography that way?
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: maryflet-at-interchange.ubc.ca [mailto:maryflet-at-interchange.ubc.ca] Sent: 24 October 2008 19:13 To: protrain-at-emcourses.com
Dear Phil, One other advantage of the "topo" BSE is that the new "table Top" SEMs are fixed variable-pressure, BSE imaging only, so the "topo" option may get a better image of the surface topography than the "compo" BSE image. Regards,
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: October 24, 2008 10:45 AM To: maryflet-at-interchange.ubc.ca
Steve,
I'm not sure I follow you. I thought I was agreeing with you. I do think SE gives higher resolution, and resolution being the sine qua non of microscopy, tney are "better". See the SEMs on: http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Progr ams/microscopy/gallery.html These were taken with an Autrata BSE, which produces near-SE quality images at 5kV. Or even 3kV. Good as they are, the SEs are still better. Keep in mind, the BSEs are also coming from a larger analyzed volume, and so have lower spatial resolution that do SEs. Even with the best BSE detector.
There is indeed a technique for a problem. That's essentially my point with using BSEs for imaging chargey samples, or for TOPO imaging of samples primarill used for COMPO BSE or x-ray.
As far as I know *all* SEMs offer dual display. X-from the JEOL 35 I learned on mumblety years ago to now. Only SEM I've ever used that didn't have dual display was a Cambridge Mk IIa.
Phil
} Hi Phil } } I do not agree that SE images are better but I am sure you would modify that } to say there is a technique for a problem? } } Most modern systems offer dual display so we can all enjoy comparing the } Everhart-Thornley image with the BSE image without complicated image mixing } boxes; progress? } } } Steve Chapman } Protrain } For training and consultancy in electron microscopy world wide } Tel +44 1280 816512 Fax +44 1280 814007 } Cell +44 7711 606967 www.emcourses.com } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: 24 October 2008 17:48 } To: protrain-at-emcourses.com } Subject: [Microscopy] Re: BSI-topo-comp : a quest. more } } } } } --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 27 -- From oshel1pe-at-cmich.edu Fri Oct 24 12:38:26 2008 7, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OHcP4w030466 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 12:38:25 -0500 7, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OHcHB0003544 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:38:21 -0400 7, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 24 Oct 2008 13:38:19 -0400 7, 27 -- Mime-Version: 1.0 7, 27 -- Message-Id: {f0624082ac527b86d43e0-at-[141.209.160.249]} 7, 27 -- In-Reply-To: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- References: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- Date: Fri, 24 Oct 2008 13:38:16 -0400 7, 27 -- To: Microscopy-at-microscopy.com 7, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 27 -- Subject: [Microscopy] RE: Re: BSI-topo-comp : a quest. more 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 27 -- X-OriginalArrivalTime: 24 Oct 2008 17:38:19.0211 (UTC) FILETIME=[4DA129B0:01C935FF] 7, 27 -- X-Canit-CHI2: 0.00 7, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 27 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 27 -- X-CanItPRO-Stream: default 7, 27 -- X-Canit-Stats-ID: 4387496 - 6ceb9297fe71 7, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OHcP4w030466 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 32 -- From maryflet-at-interchange.ubc.ca Fri Oct 24 13:12:12 2008 16, 32 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) 16, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OICBOP013364 16, 32 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:12:12 -0500 16, 32 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 16, 32 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP id 890C81EEB5 16, 32 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 11:12:09 -0700 (PDT) 16, 32 -- Received: from Mary (desk.staff.mmat.ubc.ca [137.82.16.178]) 16, 32 -- by smtp.interchange.ubc.ca 16, 32 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 16, 32 -- with ESMTP id {0K9900GFZ9889Q-at-smtp.interchange.ubc.ca} for 16, 32 -- microscopy-at-microscopy.com; Fri, 24 Oct 2008 11:12:09 -0700 (PDT) 16, 32 -- Date: Fri, 24 Oct 2008 11:12:15 -0700 16, 32 -- From: Mary Fletcher {maryflet-at-interchange.ubc.ca} 16, 32 -- Subject: RE: [Microscopy] BSI-topo-comp : a quest. more 16, 32 -- In-reply-to: {200810241744.m9OHibcb007813-at-ns.microscopy.com} 16, 32 -- To: oshel1pe-at-cmich.edu 16, 32 -- Cc: microscopy-at-microscopy.com 16, 32 -- Reply-to: maryflet-at-interchange.ubc.ca 16, 32 -- Message-id: {0K9900GG09889Q-at-smtp.interchange.ubc.ca} 16, 32 -- Organization: Materials Eng. 16, 32 -- MIME-version: 1.0 16, 32 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 16, 32 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 32 -- Content-type: text/plain; charset=us-ascii 16, 32 -- Content-transfer-encoding: 7bit 16, 32 -- Thread-index: Ack2ADAAljEjYkrITKiIjqLn8zjOxQAA428w 16, 32 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.10.24.175224 16, 32 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 16, 32 -- X-PerlMx-Spam: Probability=8%, Report=BODY_SIZE_10000_PLUS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_PRICE 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0, __USER_AGENT_MS_GENERIC 0 16, 32 -- X-Spam-Level: 16, 32 -- X-Spam-Flag: No ==============================End of - Headers==============================
==============================Original Headers============================== 27, 27 -- From protrain-at-emcourses.com Fri Oct 24 13:17:30 2008 27, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 27, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OIHTbb027193 27, 27 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:17:29 -0500 27, 27 -- Received: from 5ac8bf4d.bb.sky.com ([90.200.191.77]:3958 helo=HP6220) 27, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 27, 27 -- (envelope-from {protrain-at-emcourses.com} ) 27, 27 -- id 1KtRDx-000NfE-VY by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 24 Oct 2008 20:17:27 +0200 27, 27 -- Reply-To: {protrain-at-emcourses.com} 27, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 27, 27 -- To: {maryflet-at-interchange.ubc.ca} 27, 27 -- Cc: "Microscopy Soc America" {microscopy-at-microscopy.com} 27, 27 -- References: {200810241813.m9OID73m014836-at-ns.microscopy.com} 27, 27 -- Subject: RE: [Microscopy] RE: BSI-topo-comp : a quest. more 27, 27 -- Date: Fri, 24 Oct 2008 19:17:30 +0100 27, 27 -- Organization: Protrain 27, 27 -- Message-ID: {006e01c93604$c891b090$0200a8c0-at-HP6220} 27, 27 -- MIME-Version: 1.0 27, 27 -- Content-Type: text/plain; 27, 27 -- charset="US-ASCII" 27, 27 -- Content-Transfer-Encoding: 7bit 27, 27 -- X-Mailer: Microsoft Office Outlook 11 27, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 27, 27 -- Thread-Index: Ack2BDcpJg7kwLKXT1y+5IXkX8RJMwAAD9KQ 27, 27 -- In-Reply-To: {200810241813.m9OID73m014836-at-ns.microscopy.com} 27, 27 -- X-Scan-Signature: 07e15ca5f8d3083dd0e7bbef24b9ea4c{375}} 27, 27 -- X-Trace: smtp01.dial-up.net 1KtRDx-000NfE-VY 0807583404a872ade0dd75b96a64052c ==============================End of - Headers==============================
True, I'd forgotten about the new table-top. I suspect the original ISI table-top SEM (mid-70s, I think) also used BSE imaging, but I don't remember. Anyone recall that?
Phil
} Dear Phil, } One other advantage of the "topo" BSE is that the new "table Top" SEMs are } fixed variable-pressure, BSE imaging only, so the "topo" option may get a } better image of the surface topography than the "compo" BSE image. } Regards, } } Mary Fletcher } Electron Microscopist } Materials Eng. UBC } #309 - 6350 Stores Road } Vancouver, B.C. V6T 1Z4 } Canada } Tel: 604-822-5648 } Fax: 604-822-3619 } email: maryflet-at-interchange.ubc.ca } } } -----Original Message----- } From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: October 24, 2008 10:45 AM } To: maryflet-at-interchange.ubc.ca } Subject: [Microscopy] BSI-topo-comp : a quest. more } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 25 -- From oshel1pe-at-cmich.edu Fri Oct 24 13:39:54 2008 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OIds1p021787 4, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:39:54 -0500 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OIdltS028452 4, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 14:39:49 -0400 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 25 -- Fri, 24 Oct 2008 14:38:46 -0400 4, 25 -- Mime-Version: 1.0 4, 25 -- Message-Id: {f0624082bc527c65e8844-at-[141.209.160.249]} 4, 25 -- In-Reply-To: {0K9900GG09889Q-at-smtp.interchange.ubc.ca} 4, 25 -- References: {0K9900GG09889Q-at-smtp.interchange.ubc.ca} 4, 25 -- Date: Fri, 24 Oct 2008 14:38:44 -0400 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 25 -- Subject: RE: [Microscopy] BSI-topo-comp : a quest. more 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 25 -- X-OriginalArrivalTime: 24 Oct 2008 18:38:46.0328 (UTC) FILETIME=[BF8F4B80:01C93607] 4, 25 -- X-Canit-CHI2: 0.00 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 25 -- X-Spam-Score: -4.10 () [Hold at 5.00] L_EXCH_MF,L_USD,L_USDD,RDNS_NONE,Bayes(0.0001,-0.5) 4, 25 -- X-CanItPRO-Stream: default 4, 25 -- X-Canit-Stats-ID: 4390534 - 52338e7b5141 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Although only on rare occasions, I have used topo-bse to good effect. I have found it useful for imaging certain fracture surfaces. In some cases, it is not so much a matter of what and how much information is present, but how the image is perceived. Fine detail can be suppressed, highlighting larger fracture features. This, in concert with the suppression of light charging effects from corroded surfaces, makes some macro fracture features more apparent.
Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Fri Oct 24 13:42:14 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OIgDs9025136 2, 28 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:42:13 -0500 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.11675585; 2, 28 -- Fri, 24 Oct 2008 14:41:55 -0400 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.30]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Fri, 24 Oct 2008 14:41:55 -0400 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- Subject: RE: [Microscopy] Re: BSI-topo-comp : a quest. more 2, 28 -- Date: Fri, 24 Oct 2008 14:41:54 -0400 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7033F9772-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200810241556.m9OFu9ka032730-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] Re: BSI-topo-comp : a quest. more 2, 28 -- Thread-Index: Ack18RUHoX42Y7b+QIy/9SkfS6ZNrgAFRnkg 2, 28 -- References: {200810241556.m9OFu9ka032730-at-ns.microscopy.com} 2, 28 -- To: {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 24 Oct 2008 18:41:55.0235 (UTC) FILETIME=[30283730:01C93608] 2, 28 -- From: "White, N.W. (Woody)" {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="us-ascii" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OIgDs9025136 ==============================End of - Headers==============================
I "bought" (stole?) an ISI Mini Sem from Sherry Labs in IN back in about 1988 when they bought a new JEOL SEM and wanted to dispose of the old ISI.
I was the TRACOR service engineer at the time and I bid $100 for the unit, sans the rough pump....about two weeks later they called me and I went and picked up my new $100 toy.
A colleague and I got the unit up the next week and it worked very well up to about 15Kx and I loved it. At the time I was rebuilding a home, had twins born, and the furnace went out, so I had to sell it. It went to a furniture salesman in Toledo for $1,000 with me installing it and showing him how to use it. I believe it employed a SEI detector only, back then ISI used Robinson in the USA for any BSE and it did not have a Robinson. I could be wrong, I really only worked with EDS at the time as an engineer, and the ISI, Amray, JOEL and Hitachi engineers taught me so much in the 1984 to 1990 timeframe.
The furniture salesman from Toledo had GO V surplus items everywhere, I counted 27 "O" scopes, 4 - 16 track recording machines, and a old JOEL 100 TEM, that he thought was going to allow him to do S EM work with to play with his grandchildren. He LOVED the IS I and I wish I did not have to sell it, I lost contact with him a few years later and hope that he passed it on to a current Microcopies or generated interest in science with his grandchildren with it and all the other "toys" that he had in his HUGE play garage.
Thanks for bringing back the memory! -- Joe Ullmer
JoeXray LLC 7958 Dubois Road Carlisle, OHIO 45005 OFFICE / FAX: 937 550-9224 Cell: 937 520-3811 joexray-at-cinci.rr.com Webiste: www. Joexray.com should be up by November 10th......
==============================Original Headers============================== 7, 19 -- From joexray-at-cinci.rr.com Fri Oct 24 15:11:03 2008 7, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OKB3Kp021175 7, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Oct 2008 15:11:03 -0500 7, 19 -- Received: from hrndva-web15-z02 ([10.128.132.166]) 7, 19 -- by hrndva-smta02.mail.rr.com with ESMTP 7, 19 -- id {20081024201102.UIHC23519.hrndva-smta02.mail.rr.com-at-hrndva-web15-z02} 7, 19 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Oct 2008 20:11:02 +0000 7, 19 -- Message-ID: {23741815.518081224879063093.JavaMail.root-at-hrndva-web15-z02} 7, 19 -- Date: Fri, 24 Oct 2008 16:11:02 -0400 7, 19 -- From: {joexray-at-cinci.rr.com} 7, 19 -- To: Microscopy-at-Microscopy.Com 7, 19 -- Subject: ISI Mini Sem from 1975 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=utf-8 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- X-Priority: 3 (Normal) 7, 19 -- Sensitivity: Normal 7, 19 -- X-Originating-IP: ==============================End of - Headers==============================
In the near future I will be setting up an offsite imaging facility for which my users will be responsible for operation including the coating of their SEM specimens. Experienced staff will not be present most of the time. I am soliciting advice and your collective experiences for an end user operated sputter coater. Almost exclusively biologic materials, gold/gold:palladium preferred. Ridiculously easy/ bullet proof is the primary concern here. Most of these folks are very short term visitors and extensive training will just not be possible. Stuff in the sample, poke the button and presto ready to image is the ideal.
Thanks,
Scott Whittaker Head NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
==============================Original Headers============================== 7, 26 -- From WHITTAKS-at-si.edu Fri Oct 24 15:12:44 2008 7, 26 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OKChAp023784 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 15:12:44 -0500 7, 26 -- Received: from SI-MSESMTPO-02.US.SINET.SI.EDU (SI-MSESMTP-N2.us.sinet.si.edu [160.111.49.76]) 7, 26 -- by si-mailout03.si.edu (Postfix) with ESMTP id CA0286D16 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 16:12:43 -0400 (EDT) 7, 26 -- Received: from SI-ECL02.US.SINET.SI.EDU ([160.111.49.70]) by SI-MSESMTPO-02.US.SINET.SI.EDU with Microsoft SMTPSVC(6.0.3790.3959); 7, 26 -- Fri, 24 Oct 2008 16:12:43 -0400 7, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 26 -- Content-class: urn:content-classes:message 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; 7, 26 -- charset="US-ASCII" 7, 26 -- Subject: SEM- sputter coater recommendations 7, 26 -- Date: Fri, 24 Oct 2008 16:12:42 -0400 7, 26 -- Message-ID: {20CDD1CED2E76541A4FA119C6C806BA3030338D6-at-SI-ECL02.US.SINET.SI.EDU} 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- Thread-Topic: SEM- sputter coater recommendations 7, 26 -- Thread-Index: Ack2FN6eEf9+KkmfSDiCsTRuu27N5Q== 7, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 7, 26 -- To: {microscopy-at-microscopy.com} 7, 26 -- X-OriginalArrivalTime: 24 Oct 2008 20:12:43.0759 (UTC) FILETIME=[DFBAFFF0:01C93614] 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OKChAp023784 ==============================End of - Headers==============================
The ISI table top SEM used a conventional ET secondary detector. In fact, I still have some of the components (old scintillators, light guide, filaments, gun) that we use for teaching.
JB
oshel1pe-at-cmich.edu stated: } True, I'd forgotten about the new table-top. I suspect the original } ISI table-top SEM (mid-70s, I think) also used BSE imaging, but I } don't remember. Anyone recall that?
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I thought I would share one more method that might works for you which I used to employ for analysis of asbestos containing materials for TEM. I may be forgetting a few of the specifics, but this is the general method as I remember it.
If the client wants some type of quantitative analysis, you can suspend a known amount of the sample in a known amount of distilled water, (i.e. 0.15g in 100mL of water.) and this will allow you to keep track of how much sample makes it through the process and on to the TEM.
Get some 47mm .2um MCE (Mixed Cellulose Ester) filters from your lab supply company, and weigh each filter prior to placing them in the filtration apparatus. Use a vacuum filtration apparatus to filter various aliquots (10, 25, 50mL for example) of the original 100mL. If you need to filter small amounts of sample, add distilled water to the filtration cup and then add the sample to maximize even distribution. We used some Nalgene disposable plastic filtration cups that came pre-fitted with a course cellulose backing pad and a 5um backing filter. For best results, wet the backing pad, place the backing filter on the pad, and then the .2um filter on the backing filter. The water should soak through and all the filters should be sandwiched together with no bubbles in between. When you have finished filtering, dry each filter in a low temp oven. When the filters are completely dry, weigh the filters again, and subtract the original weight of the filter from the weight you have now to give you the amount of sample deposited on the filter.
To prepare the samples for TEM, cut out a small piece of filter (.5cmx2cm) with a scalpel or razor blade and use an acetone vaporizer to "clear" the sample on to a glass slide. You should then carbon coat the samples with a fairly thick layer of carbon to create a stable film that will retain your particles and be conductive in the electron beam. To dissolve the filter away from the carbon film and get the sample on a grid, set up a Jaffe wick consisting of a piece of fine mesh screen bent so as to form a raised platform maybe 5mm high. Place the screen in a glass petri dish, and fill it with acetone until the level of acetone is high enough that the screen wicks the acetone up into the mesh, but not so high that it is over the top of the platform. Cut out a piece of kimwipe the same shape as the platform and drape it over the mesh, and then place your grids on the kimwipe. Cut out a piece of your carbon coated sample with a razor blade and drop it on top of your grid(s) to dissolve and put the lid on the petri dish. After at least an hour, pull the kimwipe out of the petri dish and allow it to dry before picking your grids off and inspecting them in the TEM.
Everything involving acetone should of course be done in a fume hood wearing appropriate safety gear.
This is where it gets fuzzy for me; you can now use the weight of the sample, the effective area of the filter, and the area of your grids (dependent on mesh size) to come up with an amount of sample per grid opening or something like that which will allow you to figure out how many grid openings you need to look at to come up with a statistically relevant measurement. Again, I forget exactly what the goal was here, but at least you should end up with a nice even distribution of particles if nothing else. I believe this all is based on either an ISO or ASTM standard for asbestos/particle analysis, the number or reference of which I can't remember.
Hope this helps!
----- Original Message ---- X-from: "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} To: nizets2-at-yahoo.com Sent: Thursday, October 23, 2008 12:53:31 PM
Dear All, a customer wants to clean pigments (Al, Au, Fe), which are milled to a grain-size of ca. 5 micrometer or smaller. Procedure would be washing with different solutions (water, pet ether).
Problem to be solved: Pigment particles surely tend to stick together during washing and drying, the smaller the particles, the more they stick, I suppose...
On the other hand the customer needs to get as good as possible a separation of the particles to determine the overall area of the particles.
One idea to dry the particles had been to use critical point drying, but I am afraid to contaminate the dryer because I know of no confinement to use in CPD with pores smaller than one micrometer. Spreading the solution out for drying will not be enough because the customer seems to need a certain amount of mass (ca. 1 gramm) for counting...
I would be most grateful for any ideas how to proceed.
Best regards, Stefan Diller ---------------------------------------------- ---------------------------------------------- -- Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 25, 27 -- From bnross-at-interchange.ubc.ca Fri Oct 24 16:23:36 2008 25, 27 -- Received: from mr5.mail-relay.ubc.ca (mr5.mail-relay.ubc.ca [137.82.45.9]) 25, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OLNaSd002462 25, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 16:23:36 -0500 25, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 25, 27 -- by mr5.mail-relay.ubc.ca (Postfix) with ESMTP id 525181EE39 25, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 14:23:35 -0700 (PDT) 25, 27 -- Received: from handel.my.ubc.ca (handel.my.ubc.ca [137.82.115.14]) 25, 27 -- by smtp.interchange.ubc.ca 25, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 25, 27 -- with ESMTP id {0K990031FI3AUS-at-smtp.interchange.ubc.ca} for 25, 27 -- Microscopy-at-microscopy.com; Fri, 24 Oct 2008 14:23:35 -0700 (PDT) 25, 27 -- Date: Fri, 24 Oct 2008 14:23:34 -0700 (PDT) 25, 27 -- From: Bradford Ross {bnross-at-interchange.ubc.ca} 25, 27 -- Subject: Cleaning and drying of pigments. 25, 27 -- To: Microscopy-at-microscopy.com 25, 27 -- Message-id: {9447525.9481224883414298.JavaMail.myubc2-at-handel.my.ubc.ca} 25, 27 -- MIME-version: 1.0 25, 27 -- X-Mailer: uPortal WEB email client 3.0 25, 27 -- Content-type: text/plain; charset=us-ascii 25, 27 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.10.24.210138 25, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 25, 27 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_5000_5999 0, ECARD_KNOWN_DOMAINS 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_GREETING 0, __SANE_MSGID 0 25, 27 -- X-Spam-Level: 25, 27 -- X-Spam-Flag: No 25, 27 -- Content-Transfer-Encoding: 8bit 25, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OLNaSd002462 ==============================End of - Headers==============================
Thought some folks might be interested in a recent paper that Novelx presented at MNE 2008 describing a technique for using TOPO mode on its low-voltage benchtop FESEM for resolving nm steps on the surface of a crystalline substance. I will include the abstract here and should anyone be interested in a pre-print of the paper they can contact me directly and I would be glad to email it to them.
Jim Rynne Novelx, Inc. www.novelx.com rynne-at-novelx.com +1 925.962.0889 x304 (office)
Resolving sub-nm steps with a low-voltage miniature scanning electron microscope Lawrence Muray a, James Spallas a, Charles Silver a, Scott Indermuehle a Nicola Ferralis b, Carlo Carraro b, Roya Maboudian b
a Novelx, 3746 Mt. Diablo Blvd., Suite 100, Lafayette, California USA b Department of Chemical Engineering, University of California, Berkeley, California USA
Abstract Miniature scanning electron beam columns based on silicon microfabricated components and Schottky field-emission sources have been recently developed and deployed in commercial field emission scanning electron microscopes (FESEM). A second generation column, optimized for low-voltage imaging, was used to image defects in 6H-SiC using topographic mode. By comparing specific regions with AFM, steps of ~0.8nm could be identified. The images obtained with this technique are consistent with electron channeling contrast imaging (ECCI) but at significantly lower voltage, from 500eV to 1.2keV. The geometry of the detector, the collection angle, the beam convergence angle, and the source characteristics are consistent with optimum conditions for normal incidence ECCI.
-----Original Message----- X-from: maryflet-at-interchange.ubc.ca [mailto:maryflet-at-interchange.ubc.ca] Sent: Friday, October 24, 2008 11:23 AM To: rynne-at-novelx.com
Steve,
I'm not sure I follow you. I thought I was agreeing with you. I do think SE gives higher resolution, and resolution being the sine qua non of microscopy, tney are "better". See the SEMs on: http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Progr ams/microscopy/gallery.html These were taken with an Autrata BSE, which produces near-SE quality images at 5kV. Or even 3kV. Good as they are, the SEs are still better. Keep in mind, the BSEs are also coming from a larger analyzed volume, and so have lower spatial resolution that do SEs. Even with the best BSE detector.
There is indeed a technique for a problem. That's essentially my point with using BSEs for imaging chargey samples, or for TOPO imaging of samples primarill used for COMPO BSE or x-ray.
As far as I know *all* SEMs offer dual display. X-from the JEOL 35 I learned on mumblety years ago to now. Only SEM I've ever used that didn't have dual display was a Cambridge Mk IIa.
Phil
} Hi Phil } } I do not agree that SE images are better but I am sure you would modify that } to say there is a technique for a problem? } } Most modern systems offer dual display so we can all enjoy comparing the } Everhart-Thornley image with the BSE image without complicated image mixing } boxes; progress? } } } Steve Chapman } Protrain } For training and consultancy in electron microscopy world wide } Tel +44 1280 816512 Fax +44 1280 814007 } Cell +44 7711 606967 www.emcourses.com } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: 24 October 2008 17:48 } To: protrain-at-emcourses.com } Subject: [Microscopy] Re: BSI-topo-comp : a quest. more } } } } } --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 27 -- From oshel1pe-at-cmich.edu Fri Oct 24 12:38:26 2008 7, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9OHcP4w030466 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 12:38:25 -0500 7, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9OHcHB0003544 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Oct 2008 13:38:21 -0400 7, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 24 Oct 2008 13:38:19 -0400 7, 27 -- Mime-Version: 1.0 7, 27 -- Message-Id: {f0624082ac527b86d43e0-at-[141.209.160.249]} 7, 27 -- In-Reply-To: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- References: {200810241706.m9OH62Wd021283-at-ns.microscopy.com} 7, 27 -- Date: Fri, 24 Oct 2008 13:38:16 -0400 7, 27 -- To: Microscopy-at-microscopy.com 7, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 27 -- Subject: [Microscopy] RE: Re: BSI-topo-comp : a quest. more 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 27 -- X-OriginalArrivalTime: 24 Oct 2008 17:38:19.0211 (UTC) FILETIME=[4DA129B0:01C935FF] 7, 27 -- X-Canit-CHI2: 0.00 7, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 27 -- X-Spam-Score: -2.50 () [Hold at 5.00] L_EXCH_MF,L_USDD,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001,-0.5) 7, 27 -- X-CanItPRO-Stream: default 7, 27 -- X-Canit-Stats-ID: 4387496 - 6ceb9297fe71 7, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9OHcP4w030466 ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 23 -- From rynne-at-novelx.com Sat Oct 25 10:27:46 2008 25, 23 -- Received: from smtp102.biz.mail.mud.yahoo.com (smtp102.biz.mail.mud.yahoo.com [68.142.200.237]) 25, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9PFRkur024947 25, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 25 Oct 2008 10:27:46 -0500 25, 23 -- Received: (qmail 20762 invoked from network); 25 Oct 2008 15:27:45 -0000 25, 23 -- Received: from unknown (HELO Lambrusco) (rynne-at-24.4.121.7 with login) 25, 23 -- by smtp102.biz.mail.mud.yahoo.com with SMTP; 25 Oct 2008 15:27:45 -0000 25, 23 -- X-YMail-OSG: qDaXapAVM1nWKjzuddUFqRSJNWS0d.O6ccI_h9X135dxlBNFlwX7XyU.A.tDcZCsc0B6cBrBczITow64vOxaFCWfPozzJ_TC_mw8imapTh50.NYaubzICw35Y436SYK6NeBfzCWAV.pi98W6mz.G8GLnxxqEnJR1q3yxMBy3MTQ.NWPIBbTDUTIpCIc7F4X9TyngCeTul4p1UO3aCdXBkhb.26st935cqi4IdGWzJ8X6JxnsBhNrcBJbyC.r 25, 23 -- X-Yahoo-Newman-Property: ymail-3 25, 23 -- From: "Jim Rynne" {rynne-at-novelx.com} 25, 23 -- To: {Microscopy-at-microscopy.com} 25, 23 -- References: {200810241823.m9OINQ0e011460-at-ns.microscopy.com} 25, 23 -- Subject: [Microscopy] RE: Low-voltage topo: BSI-topo-comp : a quest. more 25, 23 -- Date: Sat, 25 Oct 2008 08:27:44 -0700 25, 23 -- Message-ID: {008b01c936b6$3b3e3490$0c01a8c0-at-Lambrusco} 25, 23 -- MIME-Version: 1.0 25, 23 -- Content-Type: text/plain; 25, 23 -- charset="US-ASCII" 25, 23 -- Content-Transfer-Encoding: 7bit 25, 23 -- X-Mailer: Microsoft Office Outlook 11 25, 23 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 25, 23 -- In-Reply-To: {200810241823.m9OINQ0e011460-at-ns.microscopy.com} 25, 23 -- Thread-Index: Ack2DQOffFbC9dXdQFC7dji3TL0R1AAppEWQ ==============================End of - Headers==============================
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Email: mvanbrocklin-at-stlawu.edu Name: Matt Van Brocklin
Organization: St. Lawrence University
Title-Subject: [Filtered] searching for CITL 8200 MK3 cold cathodoluminescence unit.
Question: Hello all,
I am the technician for the Geology Department at St. Lawrence University located in Canton, New York. I am searching for vendors for a CITL 8200 MK 3A cold cathodoluminescence unit that one of our faculty members is interested in pricing and potentially purchasing. Have had very little luck thus far in my search. Any suggestions as to where I might direct my search? Any information would be appreciated. Thank you for your time.
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Email: charlesping-at-hotmail.com Name: Charles
Organization: University of York
Title-Subject: [Filtered] circuit diagram for LH ion gun power supply PS-IQE 12/38
Question: Dear all
Does anyone have the circuit diagram for LH ion gun power supply PS-IQE 12/38 (867918)? The specimen current I measured is limited to around 1nA and will even drop down if I increase the emission current of the ion gun. I guess some problem with the emission current regulation of the power supply but it is difficult to tackle the problem without circuit diagram.
Many thanks
Charles Department of Electronics University of York
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Email: mvanbrocklin-at-stlawu.edu Name: Matt Van Brocklin
Organization: St. Lawrence University
Title-Subject: [Filtered] searching for CITL 8200 MK3 cold cathodoluminescence unit.
Question: Hello all,
I am the technician for the Geology Department at St. Lawrence University located in Canton, New York. I am searching for vendors for a CITL 8200 MK 3A cold cathodoluminescence unit that one of our faculty members is interested in pricing and potentially purchasing. Have had very little luck thus far in my search. Any suggestions as to where I might direct my search? Any information would be appreciated. Thank you for your time.
This is a very old power supply and no longer supported by Leybold. However you should contact them and ask for Herr Janser, he should be able to help you with a circuit diagram:
For your information: we have a new power supply for the Leybold IQE 12/38, that replaces the 867918 and the scan and deflection unit. Here is the manual for the new unit:
http://www.specs.com/products/XPS/ISC500.doc
Please feel free to contact me with any further questions. Best regards. Dietrich
} Organization: University of York } } Title-Subject: [Filtered] circuit diagram for LH ion gun power supply } PS-IQE 12/38 } } Question: Dear all } } Does anyone have the circuit diagram for LH ion gun power supply } PS-IQE 12/38 (867918)? The specimen current I measured is limited to } around 1nA and will even drop down if I increase the emission current } of the ion gun. I guess some problem with the emission current } regulation of the power supply but it is difficult to tackle the } problem without circuit diagram. } } Many thanks } } Charles } Department of Electronics } University of York
I believe that would be Cambridge Image Technology Limited, though current CL8200 model seem to be MK5:
http://www.xes84.dial.pipex.com/prod03.htm
Cheers, Valery Ray
============================ www.partbeamsystech.com www.freudlabs.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: mvanbrocklin-at-stlawu.edu [mailto:mvanbrocklin-at-stlawu.edu] Sent: Saturday, October 25, 2008 3:58 PM To: vray-at-partbeamsystech.com
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Email: mvanbrocklin-at-stlawu.edu Name: Matt Van Brocklin
Organization: St. Lawrence University
Title-Subject: [Filtered] searching for CITL 8200 MK3 cold cathodoluminescence unit.
Question: Hello all,
I am the technician for the Geology Department at St. Lawrence University located in Canton, New York. I am searching for vendors for a CITL 8200 MK 3A cold cathodoluminescence unit that one of our faculty members is interested in pricing and potentially purchasing. Have had very little luck thus far in my search. Any suggestions as to where I might direct my search? Any information would be appreciated. Thank you for your time.
I did not have any success with TUNEL labeling of EM embedded specimens several years ago (around 2000?). I spend a highly significant amount of time on this, and tried epoxy as well as cryo, UV-cured LR White embedded samples. I tried the two leading TUNEL kits available at that time. They worked on paraffin, but not on my EM samples. There was only one publication showing successful TUNEL labeling in EM at the time.
If you want specifics about what I tried, send me a personal message and I'll see what I can dig up.
~Gregg
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Friday, October 17, 2008 3:35 PM To: Sobocinski, Gregg
A colleague would like to TUNEL-label ultra-thin Epon-Araldite sections for EM. Does anyone have, or know of, any successful protocols that I may relay to her? I believe her tissue may have been fixed in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.4 (no potassium), post-fixed with osmium and en bloc-stained with uranyl acetate.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 St. Louis, MO 63110
Chee-Yeun, You've mentioned that you can't see if primary label exists due to the background staining. Now that you have staining product, reduce the concentration of your primary and secondary antibodies until you can verify that no primary staining exists behind your background staining product.
In my experience, 1:20 for a secondary is very high, so I recommend starting there. Try 1:100-1:500 first, then try diluting your primary if the background remains. Do you have any published, successful staining results to use as a starting point?
Good luck, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: cychung-at-mclean.harvard.edu [mailto:cychung-at-mclean.harvard.edu] Sent: Sunday, October 19, 2008 4:31 AM To: Sobocinski, Gregg
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both cychung-at-mclean.harvard.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Organization: McLean Hospital/Harvard Medical School
Title-Subject: [Filtered] Post-embedding immunogold staining on brain tissue for TEM: High background
Question: Post-embedding immunogold staining on brain tissue for TEM
I am working on post-embedding immunogold staining to recognize synaptic vesicles from brain tissue using TEM. EM technician in our facility have processed the tissue in following way: 1. wash with PBS 2. 1% Osmium 1 hour 3. wash with H2O 4. Dehydration 5. Embedding 6. Sectioning and placed on the nickel grids.
I have tried immunogold staining using following method: 1. 1% Sodium metaperiodate treatment 2. blocking 1 hour in 10% BSA, 3% goat serum, 0.1% fish gelatin, 0.02% triton-X 3. primary antibody (10 times higher concentration than usual) in 2% goat serum, 0.1% fish gelatin 4. wash with PBS, 0.5% BSA, 0.5% tween 20 5. secondary Ab (5 nm colloidal gold conjugate, Ted Pella) 1:20, 1 hour 30 min 6. wash with PBS, 0.5M NaCl, 0.5 % BSA. 0.5% tween 20 7. 1% glutaraldehyde fixation for 5 min. 8. wash with H2O 9. Uranyl-lead staining
Results are following: 1. Ultrastructure was well preserved 2. Nonspecific gold particles were observed in the area where there was no tissue pieces. 3. There seem to be nonspecific gold particle within the tissue as well.
As of now, due to the high background, I am not certain that I have any specific staining. If any of you have a good way to stain post-embedded tissue without background, I would greatly appreciate your advice...
Thank you so much!
Chee-Yeun
Chee-Yeun Chung, Ph.D. Instructor in Psychiatry McLean Hosptial/Harvard Medical School
Thank you to all who replied on and off list. We are lucky to have something of an expert in our department on firearms, has a background in forensic microscopy and knows his way around an SEM. He examined the sample optically. He was resonably confident to look at it our JEOL 8600. The jury is still out, but the material may well be black power. It was found in a shipwreck off the coast of Africa - a very old ship!
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 8, 31 -- From mmcheath-at-syr.edu Mon Oct 27 13:15:12 2008 8, 31 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9RIFBZh003142 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 13:15:12 -0500 8, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 31 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9RIF1bV027409 8, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 14:15:11 -0400 8, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 8, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 8, 31 -- 8.1.291.1; Mon, 27 Oct 2008 14:15:01 -0400 8, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 8, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Mon, 27 Oct 2008 14:15:01 -0400 8, 31 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu 8, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 8, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Mon, 27 Oct 8, 31 -- 2008 18:15:01 +0000 8, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 8, 31 -- Date: Mon, 27 Oct 2008 14:14:59 -0400 8, 31 -- Subject: Follow-up: Identification of gunpowder 8, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {C52B7D63.29B97%mmcheath-at-syr.edu} 8, 31 -- Thread-Topic: Follow-up: Identification of gunpowder 8, 31 -- Thread-Index: Ack4X+wL8T3dy+FZ/UajmMOK8UpSLA== 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit 8, 31 -- X-OriginalArrivalTime: 27 Oct 2008 18:15:01.0599 (UTC) FILETIME=[ED9826F0:01C9385F] 8, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-10-27_05:2008-10-10,2008-10-27,2008-10-27 signatures=0 8, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
Do any of you have experience using wire saws for very fine, precise, and controlled cut in delicate materials. We are looking at Logitechs Model 15 saw. Are there other manufacturers of this type of saw, but significantly less expensive?
Cheers Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 8, 31 -- From mmcheath-at-syr.edu Mon Oct 27 13:17:25 2008 8, 31 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9RIHPMa006910 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 13:17:25 -0500 8, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 31 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9RIHO1Y030272 8, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 14:17:24 -0400 8, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 8, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 8, 31 -- 8.1.291.1; Mon, 27 Oct 2008 14:17:24 -0400 8, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 8, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Mon, 27 Oct 2008 14:17:24 -0400 8, 31 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu 8, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 8, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Mon, 27 Oct 8, 31 -- 2008 18:17:24 +0000 8, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 8, 31 -- Date: Mon, 27 Oct 2008 14:17:23 -0400 8, 31 -- Subject: Wire saws 8, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {C52B7DF3.29B98%mmcheath-at-syr.edu} 8, 31 -- Thread-Topic: Wire saws 8, 31 -- Thread-Index: Ack4YEHg7NdCPKJff02yL36PPMVsfQ== 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit 8, 31 -- X-OriginalArrivalTime: 27 Oct 2008 18:17:24.0861 (UTC) FILETIME=[42FC32D0:01C93860] 8, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-10-27_05:2008-10-10,2008-10-27,2008-10-27 signatures=0 8, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
It is not clear what is your application and if it really requires 0.2mm cutting wire as you can use on Logitech saw, thus difficult to guess if suggestions below would be suitable for you or not, but:
1) I am using regular scroll saw with diamond-plated wire (1/32" or about 0.8mm) to cut ceramic packages of integrated circuits. Purchased from Harbor Freight Tools for under US$100 and works just fine for me; requires steady hand and patience if the package is large, head-mounted loupe is helpful if part is small or if very precise cut is needed.
2) There are many models of Diamond Band Saw available on the market, priced form just above US$200 (new) on e-bay and amazon.com up to US$1200 elsewhere, some work with diamond-plated wire and some with diamond-plated blade. I did not try these, but recall a paper presented by IBM on ISTFA a couple of years ago on using diamond band saw for the same application as I use the scroll saw - opening ceramic packages of ICs.
Cheers, Valery Ray
============================ www.partbeamsystech.com www.freudlabs.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: mmcheath-at-syr.edu [mailto:mmcheath-at-syr.edu] Sent: Monday, October 27, 2008 2:18 PM To: vray-at-partbeamsystech.com
Hi,
Do any of you have experience using wire saws for very fine, precise, and controlled cut in delicate materials. We are looking at Logitechs Model 15 saw. Are there other manufacturers of this type of saw, but significantly less expensive?
Cheers Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 8, 31 -- From mmcheath-at-syr.edu Mon Oct 27 13:17:25 2008 8, 31 -- Received: from mx5.syr.edu (mx5.syr.edu [128.230.18.67]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9RIHPMa006910 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 13:17:25 -0500 8, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 8, 31 -- by mx5.syr.edu (8.13.7/8.13.7) with ESMTP id m9RIHO1Y030272 8, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 14:17:24 -0400 8, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 8, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 8, 31 -- 8.1.291.1; Mon, 27 Oct 2008 14:17:24 -0400 8, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 8, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Mon, 27 Oct 2008 14:17:24 -0400 8, 31 -- Received: from 128.230.24.156 ([128.230.24.156]) by SUEXCL-02.ad.syr.edu 8, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 8, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Mon, 27 Oct 8, 31 -- 2008 18:17:24 +0000 8, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 8, 31 -- Date: Mon, 27 Oct 2008 14:17:23 -0400 8, 31 -- Subject: Wire saws 8, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {C52B7DF3.29B98%mmcheath-at-syr.edu} 8, 31 -- Thread-Topic: Wire saws 8, 31 -- Thread-Index: Ack4YEHg7NdCPKJff02yL36PPMVsfQ== 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit 8, 31 -- X-OriginalArrivalTime: 27 Oct 2008 18:17:24.0861 (UTC) FILETIME=[42FC32D0:01C93860] 8, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-10-27_05:2008-10-10,2008-10-27,2008-10-27 signatures=0 8, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From vray-at-partbeamsystech.com Mon Oct 27 14:28:03 2008 21, 25 -- Received: from smtp105.biz.mail.re2.yahoo.com (smtp105.biz.mail.re2.yahoo.com [206.190.52.174]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9RJS2oZ007059 21, 25 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 14:28:02 -0500 21, 25 -- Message-Id: {200810271928.m9RJS2oZ007059-at-ns.microscopy.com} 21, 25 -- Received: (qmail 29176 invoked from network); 27 Oct 2008 19:28:02 -0000 21, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) 21, 25 -- by smtp105.biz.mail.re2.yahoo.com with SMTP; 27 Oct 2008 19:28:01 -0000 21, 25 -- X-YMail-OSG: 9z.RAjcVM1k89TBksyCFjMiGcdYOC3F99Sy_fbOzH6OjCEtrqxlUNUXy27_BvNYl8O25N.JvNEMH0q.V9vOi28_r3DyRkftzsvhQxkhaYoDIUElrwb7RmUtuZRHtWHLggb53bOAlbRIr.Nn09CGURDL4ue8n4aRRqHsUljwbaxwiCS0rq2_qRoBioO7tOwF5CIM0sZx6uhIw3YovukkfRWt0DE9Vk5SJKJoRRdIzpyEDvSCAX7RJG2xLPDGfnBlaY1W.pkDF5AP4fgHZBUxN 21, 25 -- X-Yahoo-Newman-Property: ymail-3 21, 25 -- Reply-To: {vray-at-partbeamsystech.com} 21, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 21, 25 -- To: {mmcheath-at-syr.edu} 21, 25 -- Cc: {microscopy-at-microscopy.com} 21, 25 -- Subject: RE: [Microscopy] Wire saws 21, 25 -- Date: Mon, 27 Oct 2008 15:30:37 -0400 21, 25 -- Organization: PBST / MEO Engineering 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Type: text/plain; 21, 25 -- charset="us-ascii" 21, 25 -- Content-Transfer-Encoding: 7bit 21, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 21, 25 -- In-Reply-To: {200810271818.m9RIIFqq009667-at-ns.microscopy.com} 21, 25 -- Thread-Index: Ack4YGF32tcQr5tlR3SQrnwB3Vqc0gAB0Jkw 21, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
We are considering the benefits of osmium plasma coating (OPC) for non conductive low atomic weight materials with low Z height (10-100nm) using a SFEG SEM and would like to hear from other OPC users about their success vs conventional coating (carbon, platinum, gold palladium, gold, chromium)
-- Fred A. Hayes Manager, Central Facilities Department of Chemical Engineering and Material Sciences 3118 Bainer Hall Bainer Hall Drive UC Davis Davis, CA 95616 530-752-0284 office 530-754-6350 fax 707-761-9045 cell fahayes-at-ucdavis.edu http://www.matscicf.ucdavis.edu/
==============================Original Headers============================== 11, 19 -- From fahayes-at-ucdavis.edu Mon Oct 27 14:42:05 2008 11, 19 -- Received: from mx1.ucdavis.edu (mx1.ucdavis.edu [128.120.32.31]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9RJg52Z020710 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 27 Oct 2008 14:42:05 -0500 11, 19 -- Received: from [169.237.230.156] ([169.237.230.156]) 11, 19 -- by mx1.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id m9RJg3Id016173 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 27 Oct 2008 12:42:04 -0700 (PDT) 11, 19 -- Message-ID: {49061988.5000801-at-ucdavis.edu} 11, 19 -- Date: Mon, 27 Oct 2008 12:42:00 -0700 11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 11, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 11, 19 -- MIME-Version: 1.0 11, 19 -- To: Microscopy-at-Microscopy.Com 11, 19 -- Subject: FEGSEM Osmium Plasma coating 11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av7 11, 19 -- X-Virus-Status: Clean 11, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.31 ==============================End of - Headers==============================
Can some one recommend an independant SEM service engineer for the SF Bay area to service a Hitach S510 SEM?
-- Fred A. Hayes Manager, Central Facilities Department of Chemical Engineering and Material Sciences 3118 Bainer Hall Bainer Hall Drive UC Davis Davis, CA 95616 530-752-0284 office 530-754-6350 fax 707-761-9045 cell fahayes-at-ucdavis.edu http://www.matscicf.ucdavis.edu/
==============================Original Headers============================== 10, 19 -- From fahayes-at-ucdavis.edu Mon Oct 27 19:19:44 2008 10, 19 -- Received: from mx3.ucdavis.edu (mx3.ucdavis.edu [128.120.32.33]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9S0Ji8F011391 10, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 27 Oct 2008 19:19:44 -0500 10, 19 -- Received: from [169.237.230.156] ([169.237.230.156]) 10, 19 -- by mx3.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id m9S0Jhjl024467 10, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 27 Oct 2008 17:19:43 -0700 (PDT) 10, 19 -- Message-ID: {49065A9F.8090603-at-ucdavis.edu} 10, 19 -- Date: Mon, 27 Oct 2008 17:19:43 -0700 10, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 10, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Microscopy-at-Microscopy.Com 10, 19 -- Subject: looking for an independant SEM service engineer in SF Bay Area 10, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit 10, 19 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av4 10, 19 -- X-Virus-Status: Clean 10, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.33 ==============================End of - Headers==============================
I am wondering if there is anybody out there who still using Seiko 8800 dual beam FIB/SEM system and would be willing to share manuals for it. Service manuals are of the most interest, but even operator's manual would be of great help.
Thanks beforehand,
Valery Ray
============================ www.partbeamsystech.com www.freudlabs.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
==============================Original Headers============================== 7, 24 -- From vray-at-partbeamsystech.com Mon Oct 27 19:27:20 2008 7, 24 -- Received: from smtp102.biz.mail.re2.yahoo.com (smtp102.biz.mail.re2.yahoo.com [68.142.229.216]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9S0RKfg024216 7, 24 -- for {microscopy-at-microscopy.com} ; Mon, 27 Oct 2008 19:27:20 -0500 7, 24 -- Message-Id: {200810280027.m9S0RKfg024216-at-ns.microscopy.com} 7, 24 -- Received: (qmail 5453 invoked from network); 28 Oct 2008 00:27:19 -0000 7, 24 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) 7, 24 -- by smtp102.biz.mail.re2.yahoo.com with SMTP; 28 Oct 2008 00:27:19 -0000 7, 24 -- X-YMail-OSG: ZYEJTYQVM1nXqQZp0eV4V3pVDU.nXoF_JWfHrMVfJbh.XzLgFksysfJC087C4v8YzvYAP3KAKzce3nPGvOHllNvadutx0s1HzEjBLC7rpmHvA_0u7CpOPfWoWok4LajcbqAQs0LtkR8aUMP2gTAW7Gc.sGHGstiBCNOCQfFzMIrfIgDJvuhFUlPbGVEW 7, 24 -- X-Yahoo-Newman-Property: ymail-3 7, 24 -- Reply-To: {vray-at-partbeamsystech.com} 7, 24 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 7, 24 -- To: {microscopy-at-microscopy.com} 7, 24 -- Subject: RE: Seiko 8800 SEM/FIB dual-beam documentation 7, 24 -- Date: Mon, 27 Oct 2008 20:29:54 -0400 7, 24 -- Organization: PBST / MEO Engineering 7, 24 -- MIME-Version: 1.0 7, 24 -- Content-Type: text/plain; 7, 24 -- charset="us-ascii" 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 7, 24 -- Thread-Index: Ack4fCB3cHsNAYLUSqqsu1n14tOSUQAE9VlwAAENtvA= 7, 24 -- In-Reply-To: 7, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
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Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veerinary College/UPEI
Title-Subject: [Filtered] TEM image distortion
Question: Hello, I've noticed for some time now that image in our H7500 is distorted. When I look at the grid bar it has an "S" shape. The center is stright but the ends are slightly bend in oposite directions. It is visible at low to mid range magnifications. I am looking for advice what is the cause of that problem and how to correct it. TIA Dorota
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Email: gw265-at-cam.ac.uk Name: Giselle Walker
Organization: Univerrsity of Cambridge
Title-Subject: [Filtered] TEM sectioning: chloroform alternatives?
Question: In biological specimen preparation for TEM, one uses chloroform to straighten out sections (on the surface of the water bath in front of the knife). Chloroform is also used as the solvent for pioloform, one of the plastic films used to coat slot grids.
Does anyone know of something that would work like chloroform but which wouldn't be as poisonous to humans?
Does anyone have a solution for how not to end up poisoned by chloroform? Open windows, gas masks, ventilation etc don't work - try cutting 50nm serial sections in a breeze and see what happens.
Every time I come into contact with chloroform I end up with a full-blown migraine. As these tend to last about 5 days, it's a bit of a problem when I'm sectioning every day or every other day, and when my career largely depends on my skills as an electron microscopist...
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Email: john.brealey-at-imvs.sa.gov.au Name: John Brealey
Organization: SA Pathology
Title-Subject: [Filtered] Biological TEM Recommendations
Question: Hi,
Due to forces beyond our control, four diagnostic EM labs in South Australia are being rationalised into one. One good thing to come out of this is an opportunity to purchase a new TEM.
Our needs are for routine diagnostic pathology, eg, kidneys, tumours, cilia studies, fine-needle aspirate biopsies. We're not sure if it's worth incorporating an EDX function.
Any recommendations, comments, etc?
Any traps with digital cameras these days? Do new TEMs still have the capacity to take EM film? Is tilt facility standard on new TEMs?
Regards,
John Brealey Queen Elizabeth Hospital EM Unit South Australia
you really shouldn't be using chloroform in the open any more, it's just too hazardous. A risk and COSHH assessment should have been carried out in any case.
Flattening of sections is easy to answer - you just use a small portable heat pen and gently waft it just above your sections. These should be available from companies such as Agar Scientific and have been a standard piece of kit for many years in my labs.
I still use chloroform as a solvent for plastic films but I simply segregate all of the solvent handling procedures into the fume hood. I dip glass slides in a measuring cylinder containing plastic in chloroform and store them in a petri dish standing up against a small beaker and covered by a larger glass beaker while the chloroform evaporates. This can all be done in the fume hood. After about 5 minutes I remove the petri dish and beakers and cast my dry films on water in the open lab.
I hope this helps. But you must not expose yourself to chloroform it carries a fair list of hazards including: R20 Harmful by inhalation R22 Harmful if swallowed R38 Irritating to skin R40 Possible risk of irreversible effects R48 Danger of serious damage to health by prolonged exposure It is also regarded as a potential carcinogen.
You must have a fume hood somewhere nearby to handle glutaraldehyde, osmium and harmful resins anyway.
I hope this helps, but please stop using chloroform in the open and you should seek advice from Occupational Health about your past exposure.
Malcolm
Malcolm Haswell e.m. unit University of Sunderland Sunderland UK
email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: gw265-at-cam.ac.uk
We have an Amray 1645 with a four diode backscatter detector. Currently there is an issue with it and we are thinking about getting it fixed. One of the uses would be the examination of geologic thin sections, with the hopes of seeing things like zoning in minerals... If someone has a similar set up, do you get good results with it, is the system good enough (assuming it works) to get good results on thin sections...? would anyone have some example photos? I'm trying to make a case that its worth fixing (th the people with money) . Thanks, David
David A. Waugh Kent State University Department of Geology Kent, Ohio 44242 dwaugh-at-kent.edu Http://www.personal.kent.edu/~dwaugh/
==============================Original Headers============================== 6, 18 -- From dwaugh-at-kent.edu Tue Oct 28 10:30:16 2008 6, 18 -- Received: from PP02-BIGIP.uis.kent.edu (vscan.kent.edu [131.123.246.3]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SFUGM4009970 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Oct 2008 10:30:16 -0500 6, 18 -- Received: from [131.123.229.223] (bryozoansaresweet-349.geology.kent.edu [131.123.229.223]) 6, 18 -- by PP02-BIGIP.uis.kent.edu (8.14.1/8.14.1) with ESMTP id m9SFUFNJ009850 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 28 Oct 2008 11:30:15 -0400 6, 18 -- Mime-Version: 1.0 (Apple Message framework v753.1) 6, 18 -- Content-Transfer-Encoding: 7bit 6, 18 -- Message-Id: {D7044B5A-17D0-4FEA-850B-F11A24DCC5C4-at-kent.edu} 6, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 18 -- To: Microscopy-at-Microscopy.Com 6, 18 -- From: David Waugh {dwaugh-at-kent.edu} 6, 18 -- Subject: Amray backscatter 6, 18 -- Date: Tue, 28 Oct 2008 11:31:00 -0400 6, 18 -- X-Mailer: Apple Mail (2.753.1) 6, 18 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-10-28_06:2008-10-10,2008-10-28,2008-10-28 signatures=0 6, 18 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0810130000 definitions=main-0810280072 ==============================End of - Headers==============================
This is a good question to get the controversies stirred up because if the biological specimen is properly infiltrated with resin, and the resin has been correctly mixed, then you should not need to spread the sections after they have been cut - ever!
I have not used chloroform for years, purchased a battery-operated heat pen that lost its heating ability within the first weeks of use, and then had a long conversation with Hildy Crowley about embedding. She was correct in stating that full infiltration stops section compression sufficiently to make flattening unnecessary.
For really good infiltration I leave specimens for up to two weeks in uncatalyzed resin (the really difficult cells are yeast with cell walls still attached), before transferring them to resin with catalyst. Works for me.
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {gw265-at-cam.ac.uk} } Reply-To: {gw265-at-cam.ac.uk} } Date: Tue, 28 Oct 2008 03:30:45 -0500 } To: Paul Webster {PWebster-at-hei.org} } Subject: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: gw265-at-cam.ac.uk } Name: Giselle Walker } } Organization: Univerrsity of Cambridge } } Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } Question: In biological specimen preparation for TEM, one uses } chloroform to straighten out sections (on the surface of the water } bath in front of the knife). Chloroform is also used as the solvent } for pioloform, one of the plastic films used to coat slot grids. } } Does anyone know of something that would work like chloroform but } which wouldn't be as poisonous to humans? } } Does anyone have a solution for how not to end up poisoned by } chloroform? Open windows, gas masks, ventilation etc don't work - try } cutting 50nm serial sections in a breeze and see what happens. } } Every time I come into contact with chloroform I end up with a } full-blown migraine. As these tend to last about 5 days, it's a bit } of a problem when I'm sectioning every day or every other day, and } when my career largely depends on my skills as an electron } microscopist... } } Login Host: 154.20.3.125 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 18 -- From zaluzec-at-microscopy.com Tue Oct 28 03:24:57 2008 } 9, 18 -- Received: from safetwo.sceur.ch (mail-out-01.swisscom-eurospot.com } [83.97.120.90]) } 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m9S8Ouq2021692 } 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 03:24:56 -0500 } 9, 18 -- Received: from safetwo.sceur.ch (localhost.localdomain [127.0.0.1]) } 9, 18 -- by safetwo.sceur.ch (Postfix) with ESMTP id 060F33F50591 } 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:22:44 +0000 } (UTC) } 9, 18 -- Received: from [192.168.48.81] (swissco010190-2-2.clients.easynet.fr } [195.114.94.82]) } 9, 18 -- by safetwo.sceur.ch (Postfix) with ESMTP } 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:22:43 +0000 } (UTC) } 9, 18 -- Mime-Version: 1.0 } 9, 18 -- Message-Id: {p06240809c52c7cc839c4-at-[192.168.48.81]} } 9, 18 -- Date: Tue, 28 Oct 2008 03:24:58 -0500 } 9, 18 -- To: microscopy-at-microscopy.com } 9, 18 -- From: gw265-at-cam.ac.uk (by way of MicroscopyListserver) } 9, 18 -- Subject: viaWWW: TEM sectioning: chloroform alternatives? } 9, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 9, 18 -- X-DCC--Metrics: snail 1260; Body=1 Fuz1=1 Fuz2=1 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 20 -- From PWebster-at-hei.org Tue Oct 28 11:18:11 2008 10, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SGIB90026588 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 11:18:11 -0500 10, 20 -- Received: from 10.10.42.76 ([10.10.42.76]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 10, 20 -- Tue, 28 Oct 2008 16:18:09 +0000 10, 20 -- User-Agent: Microsoft-Entourage/12.13.0.080930 10, 20 -- Date: Tue, 28 Oct 2008 09:18:05 -0700 10, 20 -- Subject: Re: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? 10, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 10, 20 -- To: {gw265-at-cam.ac.uk} 10, 20 -- CC: {microscopy-at-microscopy.com} 10, 20 -- Message-ID: {C52C894D.1D57F%PWebster-at-hei.org} 10, 20 -- Thread-Topic: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? 10, 20 -- Thread-Index: Ack5GMHJyDK677emRGisXhKU/kRZtg== 10, 20 -- In-Reply-To: {200810280830.m9S8UjfI005998-at-ns.microscopy.com} 10, 20 -- Mime-version: 1.0 10, 20 -- Content-type: text/plain; 10, 20 -- charset="US-ASCII" 10, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
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Email: adler.jeremy-at-cellbio.su.se Name: Jeremy Adler
Organization: Stockholm U
Title-Subject: [Filtered] Attaching cells to aluminium
Question: We want to examine cells in a dual beam SEM. One approach would be to grow cells on aluminium stubs. Any tips about the practicalities would be appreciated.
Uncatalized resin for 2 weeks for yeast???????????????????????? Very interesting Paul. Please send me your protocol, because my HPF yeast could always be better. Do you use BDMA or DMP-30 as an accelorator. ???
Hope all is well with you. Did you get that diamond for your wife that you won at M&M?
BTW, I have changed my name from Rhonda Allen to Rhonda Trimble.
Rhonda Trimble Stowers Institute rra-at-stowers-institute.org 816-926-4346
-----Original Message----- X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org] Sent: Tuesday, October 28, 2008 11:23 AM To: Trimble, Rhonda
Hi Giselle,
This is a good question to get the controversies stirred up because if the biological specimen is properly infiltrated with resin, and the resin has been correctly mixed, then you should not need to spread the sections after they have been cut - ever!
I have not used chloroform for years, purchased a battery-operated heat pen that lost its heating ability within the first weeks of use, and then had a long conversation with Hildy Crowley about embedding. She was correct in stating that full infiltration stops section compression sufficiently to make flattening unnecessary.
For really good infiltration I leave specimens for up to two weeks in uncatalyzed resin (the really difficult cells are yeast with cell walls still attached), before transferring them to resin with catalyst. Works for me.
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {gw265-at-cam.ac.uk} } Reply-To: {gw265-at-cam.ac.uk} } Date: Tue, 28 Oct 2008 03:30:45 -0500 } To: Paul Webster {PWebster-at-hei.org} } Subject: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: gw265-at-cam.ac.uk } Name: Giselle Walker } } Organization: Univerrsity of Cambridge } } Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } Question: In biological specimen preparation for TEM, one uses } chloroform to straighten out sections (on the surface of the water } bath in front of the knife). Chloroform is also used as the solvent } for pioloform, one of the plastic films used to coat slot grids. } } Does anyone know of something that would work like chloroform but } which wouldn't be as poisonous to humans? } } Does anyone have a solution for how not to end up poisoned by } chloroform? Open windows, gas masks, ventilation etc don't work - try } cutting 50nm serial sections in a breeze and see what happens. } } Every time I come into contact with chloroform I end up with a } full-blown migraine. As these tend to last about 5 days, it's a bit of } a problem when I'm sectioning every day or every other day, and when } my career largely depends on my skills as an electron microscopist... } } Login Host: 154.20.3.125 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 9, 18 -- From zaluzec-at-microscopy.com Tue Oct 28 03:24:57 2008 } 9, 18 -- Received: from safetwo.sceur.ch (mail-out-01.swisscom-eurospot.com } [83.97.120.90]) } 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m9S8Ouq2021692 } 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 03:24:56 -0500 } 9, 18 -- Received: from safetwo.sceur.ch (localhost.localdomain [127.0.0.1]) } 9, 18 -- by safetwo.sceur.ch (Postfix) with ESMTP id 060F33F50591 } 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:22:44 +0000 } (UTC) } 9, 18 -- Received: from [192.168.48.81] (swissco010190-2-2.clients.easynet.fr } [195.114.94.82]) } 9, 18 -- by safetwo.sceur.ch (Postfix) with ESMTP } 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:22:43 +0000 } (UTC) } 9, 18 -- Mime-Version: 1.0 } 9, 18 -- Message-Id: {p06240809c52c7cc839c4-at-[192.168.48.81]} } 9, 18 -- Date: Tue, 28 Oct 2008 03:24:58 -0500 } 9, 18 -- To: microscopy-at-microscopy.com } 9, 18 -- From: gw265-at-cam.ac.uk (by way of MicroscopyListserver) } 9, 18 -- Subject: viaWWW: TEM sectioning: chloroform alternatives? } 9, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 9, 18 -- X-DCC--Metrics: snail 1260; Body=1 Fuz1=1 Fuz2=1 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 20 -- From PWebster-at-hei.org Tue Oct 28 11:18:11 2008 10, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SGIB90026588 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 11:18:11 -0500 10, 20 -- Received: from 10.10.42.76 ([10.10.42.76]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 10, 20 -- Tue, 28 Oct 2008 16:18:09 +0000 10, 20 -- User-Agent: Microsoft-Entourage/12.13.0.080930 10, 20 -- Date: Tue, 28 Oct 2008 09:18:05 -0700 10, 20 -- Subject: Re: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? 10, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 10, 20 -- To: {gw265-at-cam.ac.uk} 10, 20 -- CC: {microscopy-at-microscopy.com} 10, 20 -- Message-ID: {C52C894D.1D57F%PWebster-at-hei.org} 10, 20 -- Thread-Topic: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? 10, 20 -- Thread-Index: Ack5GMHJyDK677emRGisXhKU/kRZtg== 10, 20 -- In-Reply-To: {200810280830.m9S8UjfI005998-at-ns.microscopy.com} 10, 20 -- Mime-version: 1.0 10, 20 -- Content-type: text/plain; 10, 20 -- charset="US-ASCII" 10, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 23, 24 -- From rra-at-stowers-institute.org Tue Oct 28 12:02:25 2008 23, 24 -- Received: from stowers-institute.org (mail.stowers-institute.org [204.56.6.8]) 23, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SH2Hpo012035 23, 24 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 12:02:22 -0500 23, 24 -- From: "Trimble, Rhonda" {RRA-at-stowers-institute.org} 23, 24 -- To: "'PWebster-at-hei.org'" {PWebster-at-hei.org} 23, 24 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 23, 24 -- Date: Tue, 28 Oct 2008 12:01:52 -0500 23, 24 -- Subject: RE: [Microscopy] Re: viaWWW: TEM sectioning: chloroform 23, 24 -- alternatives? 23, 24 -- Thread-Topic: [Microscopy] Re: viaWWW: TEM sectioning: chloroform 23, 24 -- alternatives? 23, 24 -- Thread-Index: Ack5GW9IIh3SozSxSCGbl/+UfBtNdAABNBmA 23, 24 -- Message-ID: {BD62CBAC4395B94096109020651BE2EC12A148A49F-at-exchmb-02.stowers-institute.org} 23, 24 -- In-Reply-To: {200810281622.m9SGMZdR002623-at-ns.microscopy.com} 23, 24 -- Accept-Language: en-US 23, 24 -- Content-Language: en-US 23, 24 -- X-MS-Has-Attach: 23, 24 -- X-MS-TNEF-Correlator: 23, 24 -- acceptlanguage: en-US 23, 24 -- Content-Type: text/plain; charset="us-ascii" 23, 24 -- MIME-Version: 1.0 23, 24 -- Content-Transfer-Encoding: 8bit 23, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9SH2Hpo012035 ==============================End of - Headers==============================
On Oct 28, 2008, at 1:24 AM, wadowska-at-upei.ca wrote:
} I've noticed for some time now that image in our H7500 is distorted. } When I look at the grid bar it has an "S" shape. The center is } stright but the ends are slightly bend in oposite directions. It is } visible at low to mid range magnifications. I am looking for advice } what is the cause of that problem and how to correct it. } TIA
Dear Dorota, Rick Lawrence at SDSC has investigated this in detail, and he has written a program, TxBR, to correct for it and other distortions. The one you describe is called spiral distortion, and it is more bothersome at low mag and especially in very large fields of view. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Tue Oct 28 12:19:46 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SHJkxU004121 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 12:19:46 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 3E09F328A8E 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:19:46 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 27558328A83 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:19:45 -0700 (PDT) 6, 22 -- Message-Id: {354CF17D-F4A7-44F9-BD34-3E1480DBD1A3-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200810280824.m9S8Oh09021439-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] viaWWW: TEM image distortion 6, 22 -- Date: Tue, 28 Oct 2008 10:19:45 -0700 6, 22 -- References: {200810280824.m9S8Oh09021439-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
On Oct 28, 2008, at 1:25 AM, gw265-at-cam.ac.uk wrote:
} Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } Question: In biological specimen preparation for TEM, one uses } chloroform to straighten out sections (on the surface of the water } bath in front of the knife). Chloroform is also used as the solvent } for pioloform, one of the plastic films used to coat slot grids. } } Does anyone know of something that would work like chloroform but } which wouldn't be as poisonous to humans? } } Does anyone have a solution for how not to end up poisoned by } chloroform? Open windows, gas masks, ventilation etc don't work - try } cutting 50nm serial sections in a breeze and see what happens. } } Every time I come into contact with chloroform I end up with a } full-blown migraine. As these tend to last about 5 days, it's a bit } of a problem when I'm sectioning every day or every other day, and } when my career largely depends on my skills as an electron } microscopist...
Dear Giselle, I agree with Malcolm and Paul that a heat pen, or complete infiltration, is the best way to flatten sections. I will also add that all the chlorinated hydrocarbons, such as CH2Cl2, CCl4, C2H2Cl4, etc., have very similar effects on humans--especially very dangerous effects on the liver--so searching for a substitute for CHCl3 is not likely to turn up a more benign solvent. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Tue Oct 28 12:51:53 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SHprip018980 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 12:51:53 -0500 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 43A182E508C3 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:51:53 -0700 (PDT) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 376762E50BAB 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 10:51:52 -0700 (PDT) 6, 22 -- Message-Id: {F0F2D112-DA74-4FCA-882E-796FE652A5B3-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200810280825.m9S8P3fm021871-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] viaWWW: TEM sectioning: chloroform alternatives? 6, 22 -- Date: Tue, 28 Oct 2008 10:51:51 -0700 6, 22 -- References: {200810280825.m9S8P3fm021871-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Giselle, The previously mentioned heat pens work okay, although they burn through batteries pretty fast. (Pun intended)
I've previously used acetone to flatten my sections. I had a wooden applicator stick that was attached to the cap of a scintillation vial, and the stick soaked in the acetone. I waved that over my sections and they flattened pretty well. I've never used chloroform for flattening, so I can't say whether it works as well. My hands didn't need to touch the acetone, and the jar screwed tight when not in use.
Good luck, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: gw265-at-cam.ac.uk [mailto:gw265-at-cam.ac.uk] Sent: Tuesday, October 28, 2008 4:37 AM To: Sobocinski, Gregg
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: gw265-at-cam.ac.uk Name: Giselle Walker
Organization: Univerrsity of Cambridge
Title-Subject: [Filtered] TEM sectioning: chloroform alternatives?
Question: In biological specimen preparation for TEM, one uses chloroform to straighten out sections (on the surface of the water bath in front of the knife). Chloroform is also used as the solvent for pioloform, one of the plastic films used to coat slot grids.
Does anyone know of something that would work like chloroform but which wouldn't be as poisonous to humans?
Does anyone have a solution for how not to end up poisoned by chloroform? Open windows, gas masks, ventilation etc don't work - try cutting 50nm serial sections in a breeze and see what happens.
Every time I come into contact with chloroform I end up with a full-blown migraine. As these tend to last about 5 days, it's a bit of a problem when I'm sectioning every day or every other day, and when my career largely depends on my skills as an electron microscopist...
I wonder what effect block hardness might have on wrinkling or compression of sections? I would think that a harder resin formulation would result in sections with less compression vs. a softer block - harder decreases deformation? Not sure. Did Hildy Crowley say anything on this in your discussions with her? Does your resin formulation produce a harder block?
As for proper infiltration, I judge that by how the block sections, that is, smoothly, no shattering, no gross distortion, no holes - not only to the naked eye but as seen in the TEM - but heck, some compression is tolerated. If the sections show no holes or deformations at the ultrastructural level, well, to me that means proper infiltration was achieved. I do prefer a slightly softer block and so often need to use XYLENE vapors (contains no chlorine!) (applied with a toothpick attached to cork which fits into scintillation vial with few mls of xylene in it) to relax the sections (I hold my breath! after application, I wave my hand around to disperse the gas). But I guess I should try a heat pen instead. Sounds like you had a defective one?
To echo Rhonda's comment: Uncatalized resin for 2 weeks [for yeast]???????????????????????? I would add who's got THAT kind of time? Of course, one would do other things during those two weeks, but customers usually want TEM images sooner than ASAP.
Also, how long do you leave on the CATALYZED resin, to make sure that the catalyst diffuses from it into the already infiltrated uncatalyzed resin to get a desired uniform concentration of catalyst, say, 2.5% BDMA throughout the specimen? Meanwhile, the polymerization is beginning, even before you incubate at 50-60C, and so reducing catalyst diffusion rates as time goes on.
Sorry, I got a bit off the "chloroform alternative" thread here, but just want to understand why section wrinkle prevention comes down to only "proper infiltration".
Thanks, all! Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
PWebster-at-hei.org wrote: } Hi Giselle, } } This is a good question to get the controversies stirred up because if the } biological specimen is properly infiltrated with resin, and the resin has } been correctly mixed, then you should not need to spread the sections after } they have been cut - ever! } } I have not used chloroform for years, purchased a battery-operated heat pen } that lost its heating ability within the first weeks of use, and then had a } long conversation with Hildy Crowley about embedding. She was correct in } stating that full infiltration stops section compression sufficiently to } make flattening unnecessary. } } For really good infiltration I leave specimens for up to two weeks in } uncatalyzed resin (the really difficult cells are yeast with cell walls } still attached), before transferring them to resin with catalyst. Works for } me. } } Paul. } } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } } Email: gw265-at-cam.ac.uk } } Name: Giselle Walker } } } } Organization: Univerrsity of Cambridge } } } } Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } } } Question: In biological specimen preparation for TEM, one uses } } chloroform to straighten out sections (on the surface of the water } } bath in front of the knife). Chloroform is also used as the solvent } } for pioloform, one of the plastic films used to coat slot grids. } } } } Does anyone know of something that would work like chloroform but } } which wouldn't be as poisonous to humans? } } } } Does anyone have a solution for how not to end up poisoned by } } chloroform? Open windows, gas masks, ventilation etc don't work - try } } cutting 50nm serial sections in a breeze and see what happens. } } } } Every time I come into contact with chloroform I end up with a } } full-blown migraine. As these tend to last about 5 days, it's a bit } } of a problem when I'm sectioning every day or every other day, and } } when my career largely depends on my skills as an electron } } microscopist...
==============================Original Headers============================== 10, 22 -- From ahlst007-at-umn.edu Tue Oct 28 14:13:56 2008 10, 22 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SJDt2N016283 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 -0500 10, 22 -- Received: from x-160-94-173-221.cbs.umn.edu (x-160-94-173-221.cbs.umn.edu [160.94.173.221]) 10, 22 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 -0500 (CDT) 10, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-221.cbs.umn.edu [160.94.173.221] #+LO+TS+AU 10, 22 -- X-Umn-Classification: local 10, 22 -- Message-ID: {49076393.9010709-at-umn.edu} 10, 22 -- Date: Tue, 28 Oct 2008 14:10:11 -0500 10, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 10, 22 -- Reply-To: ahlst007-at-umn.edu 10, 22 -- Organization: Imaging Center UM 10, 22 -- User-Agent: Thunderbird 2.0.0.17 (Macintosh/20080914) 10, 22 -- MIME-Version: 1.0 10, 22 -- To: Microscopy-at-Microscopy.com 10, 22 -- Subject: Re: TEM sectioning: chloroform alternatives? 10, 22 -- References: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I deliberately described an extreme case to get a discussion going. Unfortunately I don't have much time to keep it going. I do need to address some of Gib's questions though.
My resin blocks are all very hard, especially compared with some of the old specimens I found in the drawers when I arrived here. I know that soft blocks work best for glass knives but we have the luxury of being able to use diamonds for almost all our sectioning (even semi-thins).
With the yeast, we have really big problems processing and embedding the unspheroplasted cells (not taking off the cell wall) even using the methods used by the yeast experts. We devised a way of embedding that worked for us and has been reproducible, However, we now have a high pressure freezer that is an idea way of "fixing" yeast, so we don't use this method any more.
Basically, the yeast are chemically fixed (formaldehyde and glutaraldehyde). Usually the cells are mailed to me after fixation, so they arrive as a suspension. They are then washed with buffer containing lysine to quench the aldehyde and embedded in either 2% gelatin or agarose. I prefer the gelatin because it is easier to step back if the cells don't centrifuge well.
The blocks are re-fixed in aldehyde to fix the gelatin (agarose) and sliced into really thin pieces. Too many yeast cells aggregating together will never infiltrat, so the thinner the slices are, the better the embedding.
The gelatin fixation and subsequent osmication, dehydration through ethanol or acetone, and first infiltration steps are performed in a microwave processor, and rushed through. Typically we get to 1:1 resin:solvent in less than 3hr. The blocks are then transferred to 1:3 (more resin, still no catalyst) and left overnight. They are then transferred to 100% resin (no catalyst) and left on a rocking machine for many days. After about a week, some blocks are taken out, mixed with fresh resin, with catalyst (BDMA), left for about 30 min, transferred to fresh, catalysed resin and baked in a 60 degree oven.
If the blocks are not well infiltrated, we go back to the specimens in the resin and embed a few more, until we get sections that can be used in the TEM. Usually we can get edges of some blocks to section sufficiently well after a few days infiltration. However, the better blocks appear after a couple of weeks.
This has all been worked out by trial and error using the very plentiful supply of yeast cultures sent to me by other people. Sometimes they did have to wait a very long time to get a result, but I did warn them in advance that they could get a more rapid result if they sent someone to do the work. I don't charge for this work so can decide to do the work on not, so the end result is on my time, not that of a paying customer.
I did some preliminary experiments with the specimens infiltrated with uncatalysed resin, because, like Gib, I was curious how the catalyst could affect tissues already filled with uncatalysed resin. Even with no pre-soak in fresh catalysed resin, much of the blocks become very hard after baking, suggesting that the catalyst is able to affect the resin already in the cells. Remember, my tip about cutting the blocks into very thin slices. I think that is the secret to how this works.
The morphology of these cells is never as good as those that have had the cell wall removed, and are not nearly as good as the high pressure frozen, freeze substituted cells.
Now, back to my manuscript submission.
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {ahlst007-at-umn.edu} } Reply-To: {ahlst007-at-umn.edu} } Date: Tue, 28 Oct 2008 14:17:12 -0500 } To: Paul Webster {PWebster-at-hei.org} } Subject: [Microscopy] Re: TEM sectioning: chloroform alternatives? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Paul, and Microsopy Listers, } } I wonder what effect block hardness might have } on wrinkling or compression of sections? I would } think that a harder resin formulation would } result in sections with less compression vs. a } softer block - harder decreases deformation? Not } sure. Did Hildy Crowley say anything on this } in your discussions with her? Does your resin } formulation produce a harder block? } } As for proper infiltration, I judge that by how } the block sections, that is, smoothly, no } shattering, no gross distortion, no holes - not } only to the naked eye but as seen in the TEM - } but heck, some compression is tolerated. If the } sections show no holes or deformations at the } ultrastructural level, well, to me that means } proper infiltration was achieved. I do prefer a } slightly softer block and so often need to use } XYLENE vapors (contains no chlorine!) (applied } with a toothpick attached to cork which fits } into scintillation vial with few mls of xylene } in it) to relax the sections (I hold my breath! } after application, I wave my hand around to } disperse the gas). But I guess I should try a } heat pen instead. Sounds like you had a } defective one? } } To echo Rhonda's comment: Uncatalized resin for } 2 weeks [for yeast]???????????????????????? I } would add who's got THAT kind of time? Of } course, one would do other things during those } two weeks, but customers usually want TEM images } sooner than ASAP. } } Also, how long do you leave on the CATALYZED } resin, to make sure that the catalyst diffuses } from it into the already infiltrated uncatalyzed } resin to get a desired uniform concentration of } catalyst, say, 2.5% BDMA throughout the } specimen? Meanwhile, the polymerization is } beginning, even before you incubate at 50-60C, } and so reducing catalyst diffusion rates as time } goes on. } } Sorry, I got a bit off the "chloroform } alternative" thread here, but just want to } understand why section wrinkle prevention comes } down to only "proper infiltration". } } Thanks, all! Gib } -- } Gilbert (Gib) Ahlstrand, Electron Microscopist, } Imaging Center, University of Minnesota } 123 Snyder Hall } St. Paul, MN 55108 } http://www.cbs.umn.edu/ic } } } PWebster-at-hei.org wrote: } } Hi Giselle, } } } } This is a good question to get the controversies stirred up because if the } } biological specimen is properly infiltrated with resin, and the resin has } } been correctly mixed, then you should not need to spread the sections after } } they have been cut - ever! } } } } I have not used chloroform for years, purchased a battery-operated heat pen } } that lost its heating ability within the first weeks of use, and then had a } } long conversation with Hildy Crowley about embedding. She was correct in } } stating that full infiltration stops section compression sufficiently to } } make flattening unnecessary. } } } } For really good infiltration I leave specimens for up to two weeks in } } uncatalyzed resin (the really difficult cells are yeast with cell walls } } still attached), before transferring them to resin with catalyst. Works for } } me. } } } } Paul. } } } } } } Paul Webster, Ph.D } } House Ear Institute } } 2100 West Third Street } } Los Angeles, CA 90057 } } (213) 273 8026 } } pwebster-at-hei.org } } } } Email: gw265-at-cam.ac.uk } } } Name: Giselle Walker } } } } } } Organization: Univerrsity of Cambridge } } } } } } Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } } } } } Question: In biological specimen preparation for TEM, one uses } } } chloroform to straighten out sections (on the surface of the water } } } bath in front of the knife). Chloroform is also used as the solvent } } } for pioloform, one of the plastic films used to coat slot grids. } } } } } } Does anyone know of something that would work like chloroform but } } } which wouldn't be as poisonous to humans? } } } } } } Does anyone have a solution for how not to end up poisoned by } } } chloroform? Open windows, gas masks, ventilation etc don't work - try } } } cutting 50nm serial sections in a breeze and see what happens. } } } } } } Every time I come into contact with chloroform I end up with a } } } full-blown migraine. As these tend to last about 5 days, it's a bit } } } of a problem when I'm sectioning every day or every other day, and } } } when my career largely depends on my skills as an electron } } } microscopist... } } } ==============================Original Headers============================== } 10, 22 -- From ahlst007-at-umn.edu Tue Oct 28 14:13:56 2008 } 10, 22 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu } [134.84.119.232]) } 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m9SJDt2N016283 } 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 -0500 } 10, 22 -- Received: from x-160-94-173-221.cbs.umn.edu } (x-160-94-173-221.cbs.umn.edu [160.94.173.221]) } 10, 22 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP } 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 -0500 } (CDT) } 10, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-221.cbs.umn.edu [160.94.173.221] } #+LO+TS+AU } 10, 22 -- X-Umn-Classification: local } 10, 22 -- Message-ID: {49076393.9010709-at-umn.edu} } 10, 22 -- Date: Tue, 28 Oct 2008 14:10:11 -0500 } 10, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} } 10, 22 -- Reply-To: ahlst007-at-umn.edu } 10, 22 -- Organization: Imaging Center UM } 10, 22 -- User-Agent: Thunderbird 2.0.0.17 (Macintosh/20080914) } 10, 22 -- MIME-Version: 1.0 } 10, 22 -- To: Microscopy-at-Microscopy.com } 10, 22 -- Subject: Re: TEM sectioning: chloroform alternatives? } 10, 22 -- References: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} } 10, 22 -- In-Reply-To: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} } 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 22 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 20 -- From PWebster-at-hei.org Tue Oct 28 14:51:58 2008 17, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SJpvVs030782 17, 20 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:51:57 -0500 17, 20 -- Received: from 10.10.42.76 ([10.10.42.76]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 17, 20 -- Tue, 28 Oct 2008 19:51:57 +0000 17, 20 -- User-Agent: Microsoft-Entourage/12.13.0.080930 17, 20 -- Date: Tue, 28 Oct 2008 12:51:53 -0700 17, 20 -- Subject: Re: [Microscopy] Re: TEM sectioning: chloroform alternatives? 17, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 17, 20 -- To: {ahlst007-at-umn.edu} 17, 20 -- CC: {Microscopy-at-Microscopy.com} 17, 20 -- Message-ID: {C52CBB69.1D5D4%PWebster-at-hei.org} 17, 20 -- Thread-Topic: [Microscopy] Re: TEM sectioning: chloroform alternatives? 17, 20 -- Thread-Index: Ack5Np/f+BcIGYN6SVOjHjp8r+Ve5w== 17, 20 -- In-Reply-To: {200810281917.m9SJHCoJ022032-at-ns.microscopy.com} 17, 20 -- Mime-version: 1.0 17, 20 -- Content-type: text/plain; 17, 20 -- charset="US-ASCII" 17, 20 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
Well, had to respond to this one. Anyone who works with plant material with even one cell layer with impermeable cell walls is used to long infiltration times. Once had a student working on resurrection plants, and infiltration of the dry material was up to 8 weeks, and even longer was better. If you want to avoid artefacts like membrane and cell wall breakage, long thought to be part of the drying down process in these plants and often discussed at length, but really just an artefact of poor fixation and infiltration, ya gotta do it.
We routinely take 2 months to process dry seeds, for example. Lots of other artefacts from too fast infiltration as well, - even in tiny Arabidopsis roots, the mature endodermis can block infiltration and result in cytorrhysis. Once had someone say to me that "roots always look like this".... well, no they don't, not if you take the time to do it properly!
These days, we try to avoid this by working on fresh or at least non-embedded material, but sometimes have to go to embedding.
cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334 ________________________________________ X-from: ahlst007-at-umn.edu [ahlst007-at-umn.edu] Sent: Wednesday, 29 October 2008 6:19 a.m. To: White, Rosemary (PI, Black Mountain)
Hi Paul, and Microsopy Listers,
I wonder what effect block hardness might have on wrinkling or compression of sections? I would think that a harder resin formulation would result in sections with less compression vs. a softer block - harder decreases deformation? Not sure. Did Hildy Crowley say anything on this in your discussions with her? Does your resin formulation produce a harder block?
As for proper infiltration, I judge that by how the block sections, that is, smoothly, no shattering, no gross distortion, no holes - not only to the naked eye but as seen in the TEM - but heck, some compression is tolerated. If the sections show no holes or deformations at the ultrastructural level, well, to me that means proper infiltration was achieved. I do prefer a slightly softer block and so often need to use XYLENE vapors (contains no chlorine!) (applied with a toothpick attached to cork which fits into scintillation vial with few mls of xylene in it) to relax the sections (I hold my breath! after application, I wave my hand around to disperse the gas). But I guess I should try a heat pen instead. Sounds like you had a defective one?
To echo Rhonda's comment: Uncatalized resin for 2 weeks [for yeast]???????????????????????? I would add who's got THAT kind of time? Of course, one would do other things during those two weeks, but customers usually want TEM images sooner than ASAP.
Also, how long do you leave on the CATALYZED resin, to make sure that the catalyst diffuses from it into the already infiltrated uncatalyzed resin to get a desired uniform concentration of catalyst, say, 2.5% BDMA throughout the specimen? Meanwhile, the polymerization is beginning, even before you incubate at 50-60C, and so reducing catalyst diffusion rates as time goes on.
Sorry, I got a bit off the "chloroform alternative" thread here, but just want to understand why section wrinkle prevention comes down to only "proper infiltration".
Thanks, all! Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
PWebster-at-hei.org wrote: } Hi Giselle, } } This is a good question to get the controversies stirred up because if the } biological specimen is properly infiltrated with resin, and the resin has } been correctly mixed, then you should not need to spread the sections after } they have been cut - ever! } } I have not used chloroform for years, purchased a battery-operated heat pen } that lost its heating ability within the first weeks of use, and then had a } long conversation with Hildy Crowley about embedding. She was correct in } stating that full infiltration stops section compression sufficiently to } make flattening unnecessary. } } For really good infiltration I leave specimens for up to two weeks in } uncatalyzed resin (the really difficult cells are yeast with cell walls } still attached), before transferring them to resin with catalyst. Works for } me. } } Paul. } } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } } Email: gw265-at-cam.ac.uk } } Name: Giselle Walker } } } } Organization: Univerrsity of Cambridge } } } } Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } } } Question: In biological specimen preparation for TEM, one uses } } chloroform to straighten out sections (on the surface of the water } } bath in front of the knife). Chloroform is also used as the solvent } } for pioloform, one of the plastic films used to coat slot grids. } } } } Does anyone know of something that would work like chloroform but } } which wouldn't be as poisonous to humans? } } } } Does anyone have a solution for how not to end up poisoned by } } chloroform? Open windows, gas masks, ventilation etc don't work - try } } cutting 50nm serial sections in a breeze and see what happens. } } } } Every time I come into contact with chloroform I end up with a } } full-blown migraine. As these tend to last about 5 days, it's a bit } } of a problem when I'm sectioning every day or every other day, and } } when my career largely depends on my skills as an electron } } microscopist...
==============================Original Headers============================== 10, 22 -- From ahlst007-at-umn.edu Tue Oct 28 14:13:56 2008 10, 22 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SJDt2N016283 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 -0500 10, 22 -- Received: from x-160-94-173-221.cbs.umn.edu (x-160-94-173-221.cbs.umn.edu [160.94.173.221]) 10, 22 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 -0500 (CDT) 10, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-221.cbs.umn.edu [160.94.173.221] #+LO+TS+AU 10, 22 -- X-Umn-Classification: local 10, 22 -- Message-ID: {49076393.9010709-at-umn.edu} 10, 22 -- Date: Tue, 28 Oct 2008 14:10:11 -0500 10, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 10, 22 -- Reply-To: ahlst007-at-umn.edu 10, 22 -- Organization: Imaging Center UM 10, 22 -- User-Agent: Thunderbird 2.0.0.17 (Macintosh/20080914) 10, 22 -- MIME-Version: 1.0 10, 22 -- To: Microscopy-at-Microscopy.com 10, 22 -- Subject: Re: TEM sectioning: chloroform alternatives? 10, 22 -- References: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have had similar comments about yeast (...always looking like this). In my case, it was that yeast are too osmiophilic to embed in epoxy resin. The reason for this conclusion is, I guess, that un-embedded yeast will take up large amounts of uranyl acetate and lead citrate, so look too dark in the EM.
Embed the cells well and they take up less contrasting agent. By the way, I don't think I ever tried embedding yeast in Spurr's resin. This may work even better.
One thing I do know, the yeast cytoplasm is packed full of stuff so it is difficult to get the contrast expected of mammalian cells.
Regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {Rosemary.White-at-csiro.au} } Reply-To: {Rosemary.White-at-csiro.au} } Date: Tue, 28 Oct 2008 16:19:02 -0500 } To: Paul Webster {PWebster-at-hei.org} } Subject: [Microscopy] Re: TEM sectioning: infiltration thread } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Well, had to respond to this one. Anyone who works with plant } material with even one cell layer with impermeable cell walls } is used to long infiltration times. Once had a student working } on resurrection plants, and infiltration of the dry material was } up to 8 weeks, and even longer was better. If you want to avoid } artefacts like membrane and cell wall breakage, long thought to } be part of the drying down process in these plants and often } discussed at length, but really just an artefact of poor fixation } and infiltration, ya gotta do it. } } We routinely take 2 months to process dry seeds, for example. } Lots of other artefacts from too fast infiltration as well, - even } in tiny Arabidopsis roots, the mature endodermis can block } infiltration and result in cytorrhysis. Once had someone say to } me that "roots always look like this".... well, no they don't, not } if you take the time to do it properly! } } These days, we try to avoid this by working on fresh or at least } non-embedded material, but sometimes have to go to embedding. } } cheers, } Rosemary } } Rosemary White } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } ph 61 2 6246 5475 } fx 61 2 6246 5334 } ________________________________________ } X-from: ahlst007-at-umn.edu [ahlst007-at-umn.edu] } Sent: Wednesday, 29 October 2008 6:19 a.m. } To: White, Rosemary (PI, Black Mountain) } Subject: [Microscopy] Re: TEM sectioning: chloroform alternatives? } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Paul, and Microsopy Listers, } } I wonder what effect block hardness might have } on wrinkling or compression of sections? I would } think that a harder resin formulation would } result in sections with less compression vs. a } softer block - harder decreases deformation? Not } sure. Did Hildy Crowley say anything on this } in your discussions with her? Does your resin } formulation produce a harder block? } } As for proper infiltration, I judge that by how } the block sections, that is, smoothly, no } shattering, no gross distortion, no holes - not } only to the naked eye but as seen in the TEM - } but heck, some compression is tolerated. If the } sections show no holes or deformations at the } ultrastructural level, well, to me that means } proper infiltration was achieved. I do prefer a } slightly softer block and so often need to use } XYLENE vapors (contains no chlorine!) (applied } with a toothpick attached to cork which fits } into scintillation vial with few mls of xylene } in it) to relax the sections (I hold my breath! } after application, I wave my hand around to } disperse the gas). But I guess I should try a } heat pen instead. Sounds like you had a } defective one? } } To echo Rhonda's comment: Uncatalized resin for } 2 weeks [for yeast]???????????????????????? I } would add who's got THAT kind of time? Of } course, one would do other things during those } two weeks, but customers usually want TEM images } sooner than ASAP. } } Also, how long do you leave on the CATALYZED } resin, to make sure that the catalyst diffuses } from it into the already infiltrated uncatalyzed } resin to get a desired uniform concentration of } catalyst, say, 2.5% BDMA throughout the } specimen? Meanwhile, the polymerization is } beginning, even before you incubate at 50-60C, } and so reducing catalyst diffusion rates as time } goes on. } } Sorry, I got a bit off the "chloroform } alternative" thread here, but just want to } understand why section wrinkle prevention comes } down to only "proper infiltration". } } Thanks, all! Gib } -- } Gilbert (Gib) Ahlstrand, Electron Microscopist, } Imaging Center, University of Minnesota } 123 Snyder Hall } St. Paul, MN 55108 } http://www.cbs.umn.edu/ic } } } PWebster-at-hei.org wrote: } } Hi Giselle, } } } } This is a good question to get the controversies stirred up because if the } } biological specimen is properly infiltrated with resin, and the resin has } } been correctly mixed, then you should not need to spread the sections after } } they have been cut - ever! } } } } I have not used chloroform for years, purchased a battery-operated heat pen } } that lost its heating ability within the first weeks of use, and then had a } } long conversation with Hildy Crowley about embedding. She was correct in } } stating that full infiltration stops section compression sufficiently to } } make flattening unnecessary. } } } } For really good infiltration I leave specimens for up to two weeks in } } uncatalyzed resin (the really difficult cells are yeast with cell walls } } still attached), before transferring them to resin with catalyst. Works for } } me. } } } } Paul. } } } } } } Paul Webster, Ph.D } } House Ear Institute } } 2100 West Third Street } } Los Angeles, CA 90057 } } (213) 273 8026 } } pwebster-at-hei.org } } } } Email: gw265-at-cam.ac.uk } } } Name: Giselle Walker } } } } } } Organization: Univerrsity of Cambridge } } } } } } Title-Subject: [Filtered] TEM sectioning: chloroform alternatives? } } } } } } Question: In biological specimen preparation for TEM, one uses } } } chloroform to straighten out sections (on the surface of the water } } } bath in front of the knife). Chloroform is also used as the solvent } } } for pioloform, one of the plastic films used to coat slot grids. } } } } } } Does anyone know of something that would work like chloroform but } } } which wouldn't be as poisonous to humans? } } } } } } Does anyone have a solution for how not to end up poisoned by } } } chloroform? Open windows, gas masks, ventilation etc don't work - try } } } cutting 50nm serial sections in a breeze and see what happens. } } } } } } Every time I come into contact with chloroform I end up with a } } } full-blown migraine. As these tend to last about 5 days, it's a bit } } } of a problem when I'm sectioning every day or every other day, and } } } when my career largely depends on my skills as an electron } } } microscopist... } } } ==============================Original Headers============================== } 10, 22 -- From ahlst007-at-umn.edu Tue Oct 28 14:13:56 2008 } 10, 22 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu } [134.84.119.232]) } 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m9SJDt2N016283 } 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 } -0500 } 10, 22 -- Received: from x-160-94-173-221.cbs.umn.edu } (x-160-94-173-221.cbs.umn.edu [160.94.173.221]) } 10, 22 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP } 10, 22 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 14:13:55 } -0500 (CDT) } 10, 22 -- X-Umn-Remote-Mta: [N] x-160-94-173-221.cbs.umn.edu [160.94.173.221] } #+LO+TS+AU } 10, 22 -- X-Umn-Classification: local } 10, 22 -- Message-ID: {49076393.9010709-at-umn.edu} } 10, 22 -- Date: Tue, 28 Oct 2008 14:10:11 -0500 } 10, 22 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} } 10, 22 -- Reply-To: ahlst007-at-umn.edu } 10, 22 -- Organization: Imaging Center UM } 10, 22 -- User-Agent: Thunderbird 2.0.0.17 (Macintosh/20080914) } 10, 22 -- MIME-Version: 1.0 } 10, 22 -- To: Microscopy-at-Microscopy.com } 10, 22 -- Subject: Re: TEM sectioning: chloroform alternatives? } 10, 22 -- References: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} } 10, 22 -- In-Reply-To: {200810281621.m9SGL8eG031543-at-ns.microscopy.com} } 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 22 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 19, 46 -- From prvs=Rosemary.White=18033fcad-at-csiro.au Tue Oct 28 16:15:38 2008 } 19, 46 -- Received: from vic-MTAout4.csiro.au (vic-MTAout4.csiro.au } [150.229.64.41]) } 19, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m9SLFaPK014378 } 19, 46 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 16:15:37 -0500 } 19, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 19, 46 -- d=csiro.au; i=Rosemary.White-at-csiro.au; q=dns/txt; } 19, 46 -- s=email; t=1225228537; x=1256764537; } 19, 46 -- h=from:sender:reply-to:subject:date:message-id:to:cc: } 19, 46 -- mime-version:content-transfer-encoding:content-id: } 19, 46 -- content-description:resent-date:resent-from:resent-sender: } 19, 46 -- resent-to:resent-cc:resent-message-id:in-reply-to: } 19, 46 -- references:list-id:list-help:list-unsubscribe: } 19, 46 -- list-subscribe:list-post:list-owner:list-archive; } 19, 46 -- z=From:=20 {Rosemary.White-at-csiro.au} |Subject:=20Re:=20TEM } 19, 46 -- =20sectioning:=20infiltration=20thread|Date:=20Wed,=2029 } 19, 46 -- =20Oct=202008=2008:15:32=20+1100|Message-ID:=20 {0A5E41F72 } 19, 46 -- 813C247889F7974C8D764955BF03041FE-at-exvic-mbx04.nexus.csiro } 19, 46 -- .au} |To:=20 {Microscopy-at-Microscopy.com} |MIME-Version:=201. } 19, 46 -- 0|Content-Transfer-Encoding:=20quoted-printable; } 19, 46 -- bh=gRfZwx6p0PUwxHga56/a76owL35XS7+1uOgekzbd8Po=; } 19, 46 -- b=JY0pMP7pmHmPEXfKIjQ4diuVt7wxsDa1remjO6r+knjQtAwSDGG0LxwY } 19, 46 -- Fc9iW/0ZMGj8lwKPSVc1If6EZaRdmZnlA9gnxQ5bJ862eM+Pn+8jlVHOn } 19, 46 -- O6yvNjJt4lrO3Bs; } 19, 46 -- X-IronPort-AV: E=Sophos;i="4.33,501,1220191200"; } 19, 46 -- d="scan'208";a="2244361" } 19, 46 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127]) } 19, 46 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 29 Oct 2008 } 08:15:34 +1100 } 19, 46 -- Received: from exvic-mbx04.nexus.csiro.au ([138.194.81.124]) by } 19, 46 -- exvic-htca02.nexus.csiro.au ([138.194.81.127]) with mapi; Wed, 29 } Oct 2008 } 19, 46 -- 08:15:33 +1100 } 19, 46 -- From: {Rosemary.White-at-csiro.au} } 19, 46 -- To: {Microscopy-at-microscopy.com} } 19, 46 -- Date: Wed, 29 Oct 2008 08:15:32 +1100 } 19, 46 -- Subject: Re: TEM sectioning: infiltration thread } 19, 46 -- Thread-Topic: TEM sectioning: infiltration thread } 19, 46 -- Thread-Index: AQHJOUJPhf7qr92lckC3TJ/BzSFBUA== } 19, 46 -- Message-ID: } {0A5E41F72813C247889F7974C8D764955BF03041FE-at-exvic-mbx04.nexus.csiro.au} } 19, 46 -- Accept-Language: en-US, en-AU } 19, 46 -- Content-Language: en-NZ } 19, 46 -- X-MS-Has-Attach: } 19, 46 -- X-MS-TNEF-Correlator: } 19, 46 -- acceptlanguage: en-US, en-AU } 19, 46 -- Content-Type: text/plain; charset="us-ascii" } 19, 46 -- MIME-Version: 1.0 } 19, 46 -- Content-Transfer-Encoding: 8bit } 19, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m9SLFaPK014378 } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 19 -- From PWebster-at-hei.org Tue Oct 28 16:45:01 2008 10, 19 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9SLj19R028554 10, 19 -- for {Microscopy-at-Microscopy.com} ; Tue, 28 Oct 2008 16:45:01 -0500 10, 19 -- Received: from 10.10.42.76 ([10.10.42.76]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 10, 19 -- Tue, 28 Oct 2008 21:45:00 +0000 10, 19 -- User-Agent: Microsoft-Entourage/12.13.0.080930 10, 19 -- Date: Tue, 28 Oct 2008 14:44:51 -0700 10, 19 -- Subject: Re: [Microscopy] Re: TEM sectioning: infiltration thread 10, 19 -- From: "Webster, Paul" {PWebster-at-hei.org} 10, 19 -- To: {Rosemary.White-at-csiro.au} , {Microscopy-at-Microscopy.com} 10, 19 -- Message-ID: {C52CD5E3.1D5E8%PWebster-at-hei.org} 10, 19 -- Thread-Topic: [Microscopy] Re: TEM sectioning: infiltration thread 10, 19 -- Thread-Index: Ack5Rmfggql0LBeOTRWalZti7WKR/g== 10, 19 -- In-Reply-To: {200810282119.m9SLJ2Ia020190-at-ns.microscopy.com} 10, 19 -- Mime-version: 1.0 10, 19 -- Content-type: text/plain; 10, 19 -- charset="US-ASCII" 10, 19 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] Problem with Robinson BSE Detector
Question: Hello,
I have a Robinson Backscatter Detector attached to a Hitachi S-3500N SEM and I'm having a little problem with the images it's giving me. I'm analyzing diesel soot filter cross-sections and the channels of the filter in the images are lighter on one side than on the other. Even the pores in the filter are "illuminated" on the right side and tops of the pores. I've tried aligning the aperature and that wasn't the problem. I've tried increasing the beam current, as the Hitachi technician suggested, but that didn't completely solve the problem. He seemed to suggest that this may be normal with the Robinson detector, but I'm having trouble with the analysis with the illuminated sides. I know that it isn't the actual sample that has some elemental distribution on the sides because when I turn the image it's always the right side that is illuminated. Has anyone else had this kind of problem, or does anyone know the cause and solution to this? Thanks.
Yes, your engineer is right. That is normal behavior for a Robinson detector.
We have a Robinson on our Hitachi S-2460N. It is mounted on the left side of the chamber. The left side of the detector seems to have a bit more area. Therefore, the image appears as if illuminated from the left and the right sides of the holes appear a bit brighter. Similarly the left sides of bumps appear brighter.
Those comments are for the case where the raster is rotated so that things on the left side of the chamber appear on the left side of the screen. If you were to rotate the raster, the bright and dark areas should rotate around. If you rotate the sample (while leaving the raster set), the shading should maintain its orientation and the right side of the holes should remain light.
Even in cases where there is no topography, we still see some offset in the brightness of the signal towards the left. I don't know if that is due to the center of the detector being shifted or the detector being a bit thicker and closer to the sample on that side.
For that reason, we purchased an after-market solid-state detector. It mounts coaxially with the beam and avoids shading. It is also more sensitive at low voltages. However, it is a bit slower. The Robinson is a good detector and useful at faster scanning rates, but we rarely use it.
I hope this helps. Warren S.
-----Original Message----- X-from: kristi.majni-at-basf.com [mailto:kristi.majni-at-basf.com] Sent: Tuesday, October 28, 2008 4:52 PM To: wesaia-at-iastate.edu
That's an interesting situation. I don't see any difference in brightness from side to side unless there is some topology effect via the specimen and where the beam hits. The scintillator collector relies on the plastic light pipe to transport photons to the PMT. In its geometry, the furthest side of the scintillator is further from the light pipe feed to the PMT. But the difference is rather small it would seem to be an issue.
If the scintillator is unevenly dirty, that will of course affect brightness across the scan.
The detector ought to always be mounted on the side that supports the highest amount of specimen tilt. If the specimen is tilted, what happens to the image? Also, what does the SE image look like relative to BSE? If a scan is made of Au on C, does it also have uneven brightness? Strange.
gary g.
At 03:33 PM 10/28/2008, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Tue Oct 28 18:09:47 2008 10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9SN9kPw007579 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 18:09:47 -0500 10, 20 -- Message-Id: {200810282309.m9SN9kPw007579-at-ns.microscopy.com} 10, 20 -- Received: (qmail 23280 invoked from network); 28 Oct 2008 16:01:33 -0700 10, 20 -- Received: by simscan 1.1.0 ppid: 23277, pid: 23278, t: 0.1240s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 20 -- by smtp2 with SMTP; 28 Oct 2008 16:01:33 -0700 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Tue, 28 Oct 2008 16:09:42 -0700 10, 20 -- To: wesaia-at-iastate.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] RE: viaWWW: Problem with Robinson BSE Detector 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} , kristi.majni-at-basf.com 10, 20 -- In-Reply-To: {200810282233.m9SMX3IC026627-at-ns.microscopy.com} 10, 20 -- References: {200810282233.m9SMX3IC026627-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I am in the midst of teaching a bunch of beginning EM students a course that goes by the name of 'Digital Imaging'.
I have been covering the usual, digital images, bit depth, resolution, yada yada yada. We did some Photoshop, but I would like to make this more than just a Photoshop class. I need advice about what you as an employer or PI might expect to see in a job applicant. What would you expect someone to know if you saw a transcript with a digital imaging class listed? I learned digital imaging on a 'learn as you go' basis, so having to fill up a whole class is a challenge. Any ideas for projects or other assignments? This is a work in progress, so there will be time for it to evolve.
Part two has to do with Image J. I used to be pretty slick with NIH Image, but feel not so slick with Image J. Any hints on how to make the transition less painful? Right now I am trying to get the 'shading correction' plug-in to work and having no luck. What is the secret?
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 36 -- From jkrupp-at-deltacollege.edu Tue Oct 28 18:38:53 2008 9, 36 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m9SNcqRT021428 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 18:38:53 -0500 9, 36 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 9, 36 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 9, 36 -- with ESMTP id 43249019 for microscopy-at-microscopy.com; Tue, 28 Oct 2008 16:38:52 -0700 9, 36 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 9, 36 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 9, 36 -- ESMTP id K9H2D300.KWS for {microscopy-at-microscopy.com} ; Tue, 28 9, 36 -- Oct 2008 16:24:39 -0700 9, 36 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id F12488F74EA4 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 16:38:51 -0700 (PDT) 9, 36 -- X-Virus-Scanned: amavisd-new at 9, 36 -- X-Spam-Flag: NO 9, 36 -- X-Spam-Score: -2.499 9, 36 -- X-Spam-Level: 9, 36 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 9, 36 -- BAYES_00=-2.599, RDNS_NONE=0.1] 9, 36 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 36 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 9, 36 -- with ESMTP id VGY8eK7pGP59 for {microscopy-at-microscopy.com} ; 9, 36 -- Tue, 28 Oct 2008 16:38:47 -0700 (PDT) 9, 36 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) 9, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id AA0A48F74E80 9, 36 -- for {microscopy-at-microscopy.com} ; Tue, 28 Oct 2008 16:38:47 -0700 (PDT) 9, 36 -- Message-Id: {99798C0E-3C49-43FD-997A-DEF768DA7799-at-deltacollege.edu} 9, 36 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 9, 36 -- To: microscopy-at-microscopy.com 9, 36 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 36 -- Content-Transfer-Encoding: 7bit 9, 36 -- Mime-Version: 1.0 (Apple Message framework v929.2) 9, 36 -- Subject: Digital imaging and shading correction 9, 36 -- Date: Tue, 28 Oct 2008 16:38:47 -0700 9, 36 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I just want to thank those who replied to my question about biological TEMs. You've given me some really good info that I can share with my colleagues. Everyone seems to agree about retaining a film capability (if even only for back-up). To Linda, we currently have a Hitachi H-600 and the filament life is very good, as you say.
Regards,
John Brealey EM Unit Queen Elizabeth Hospital Adelaide South Australia
==============================Original Headers============================== 5, 35 -- From john.brealey-at-imvs.sa.gov.au Wed Oct 29 01:03:48 2008 5, 35 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 5, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9T63lE7026384 5, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 01:03:48 -0500 5, 35 -- X-IronPort-AV: E=Sophos;i="4.33,505,1220193000"; 5, 35 -- d="scan'208";a="4362440" 5, 35 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88]) 5, 35 -- by mailgate9.sa.gov.au with ESMTP; 29 Oct 2008 16:33:46 +1030 5, 35 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 5, 35 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id 5, 35 -- 8.1.263.0; Wed, 29 Oct 2008 16:33:52 +1030 5, 35 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) by 5, 35 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id A3B2A34007 for 5, 35 -- {Microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 16:33:38 +1030 (CST) 5, 35 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 5, 35 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) by localhost 5, 35 -- (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) with LMTP id 5, 35 -- oErzfBEdtADP for {Microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 16:33:32 +1030 5, 35 -- (CST) 5, 35 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 5, 35 -- ablett.imvs.sa.gov.au (Postfix) with SMTP id 9E28034044 for 5, 35 -- {Microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 16:33:32 +1030 (CST) 5, 35 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 5, 35 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 5, 35 -- To: {Microscopy-at-microscopy.com} 5, 35 -- Subject: Biological TEM - Thanks 5, 35 -- Date: Wed, 29 Oct 2008 16:33:31 +1030 5, 35 -- Organization: IMVS 5, 35 -- Message-ID: {000901c9398c$12948de0$7d8a140a-at-41347i} 5, 35 -- MIME-Version: 1.0 5, 35 -- Content-Type: text/plain; charset="us-ascii" 5, 35 -- Content-Transfer-Encoding: 7bit 5, 35 -- X-Mailer: Microsoft Office Outlook 11 5, 35 -- Thread-Index: Ack5jBHr6xHO8NxYQh+kgDU3y1RQHA== 5, 35 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
The Robinson Backscatter Detector will show directional preference due to the geometry of the detector itself. Physically it is shaped like a fork with one end open and can not detect electrons in the open portion of the detector. Hence; the overall detection efficiency is skewed toward the base and features in that direction will be more intense. Unless you block an equal portion of detector shape and area on the base side it will continue to exhibit these tendencies. Fran Laabs Ames Laboratory, ISU
At 05:32 PM 10/28/2008, you wrote:
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==============================Original Headers============================== 8, 25 -- From fclaabs-at-iastate.edu Wed Oct 29 08:55:57 2008 8, 25 -- Received: from mailhub-4.iastate.edu (mailhub-4.iastate.edu [129.186.140.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9TDtvIp026750 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 08:55:57 -0500 8, 25 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 8, 25 -- by mailhub-4.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m9TDtoJb021430; 8, 25 -- Wed, 29 Oct 2008 08:55:51 -0500 8, 25 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 8, 25 -- id 4542_65b5e708_a5c0_11dd_97ea_00137253420a; 8, 25 -- Wed, 29 Oct 2008 08:49:24 -0500 8, 25 -- Received: from oldtsl.iastate.edu (mach-147-155-184-124.pub.ameslab.gov [147.155.184.124]) 8, 25 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id m9TDtnfw030129; 8, 25 -- Wed, 29 Oct 2008 08:55:49 -0500 8, 25 -- Message-Id: {200810291355.m9TDtnfw030129-at-despam-10.iastate.edu} 8, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 25 -- Date: Wed, 29 Oct 2008 08:55:50 -0500 8, 25 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} , kristi.majni-at-basf.com 8, 25 -- From: Francis Laabs {fclaabs-at-iastate.edu} 8, 25 -- Subject: Re: [Microscopy] RE: viaWWW: Problem with Robinson BSE Detector 8, 25 -- In-Reply-To: {200810282232.m9SMWq2M026299-at-ns.microscopy.com} 8, 25 -- References: {200810282232.m9SMWq2M026299-at-ns.microscopy.com} 8, 25 -- Mime-Version: 1.0 8, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 25 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.10.29.133712 8, 25 -- X-ISUMailhub-test: Gauge=X, Probability=11%, Report='LINES_OF_YELLING_3 0.671, X_MAILER_EUDORA_7109 0.05, BODY_SIZE_7000_7999 0, RDNS_GENERIC_POOLED 0, RDNS_SUSP 0, RDNS_SUSP_GENERIC 0, __ANY_QUALCOMM_MUA 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __FRAUD_419_CONTACT_NAME 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RU_812 0, __LINES_OF_YELLING 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __STOCK_PHRASE_24 0' ==============================End of - Headers==============================
John, I think one of the primary goals is to instruct the students on use of the camera software. Nowadays the software out there is so dynamic that it can do tons of things, (i.e. montage etc). You might check with the manufacturers as they may have an educational edition so you can allow the students to use a modified version at their computer stations as a group. Also, since manipulation of digital images is limited, you might instruct them in what is acceptable for scientific images and what is not. Another item might be how to calculate and "burn in" micron markers so they are accurate for images which are cropped, enlarged etc. They may someday need to create images for a paper or poster. They need to know what are the limitations of resolutions for a poster? Paper? How to create insets? Students also need to learn how to make figure legends and how to label structures on their images neatly and accurately. A suggestion for a project might be to make a poster of their already saved images from other classes or taking one of their images, labeling the structures and putting the images on the same page as the figure legend--no cutting etc. Hope this helps! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {jkrupp-at-deltacollege.edu} To: {pekysar-at-ucdavis.edu} Sent: Tuesday, October 28, 2008 3:44 PM
TEM Researchers,
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This position will help train the researchers at U of O on Titan. They will be the tool "expert" onsite and will directly help in some of U of O's major research efforts. This position will last for about 1 year and will then move to support another US based research facility (yet to be named). This is a full time permanent position and we can help with relocation. Please note that this position will reside in Eugene, Oregon for about the first year and then move to another location within the US. Must be open to travel.
If you are interested, please go to
http://careers.fei.com/DetailFEI.asp?fei1374-HBO
and review the full job description. We are requiring an extensive background doing research with TEMs along with EELS, and corrector experience.
Best Regards,
Joe Williamson Sr. Technical Recruiter FEI Company 5350 NE Dawson Creek Drive Hillsboro, OR 97124 (Ph) 503.726.2639 | 800.334.1403 Joe.Williamson-at-fei.com
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==============================Original Headers============================== 11, 28 -- From Resume-at-fei.com Wed Oct 29 12:51:49 2008 11, 28 -- Received: from smtp.feico.com (smtp.feico.com [207.170.206.83]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9THpmCq003797 11, 28 -- for {Microscopy-at-Microscopy.Com} ; Wed, 29 Oct 2008 12:51:49 -0500 11, 28 -- X-WSS-ID: 0K9IHMD-01-K9D-01 11, 28 -- Received: from hlexc05.w2k.feico.com (unknown [10.150.40.134]) 11, 28 -- by smtp.feico.com (Tumbleweed MailGate 3.5.1) with ESMTP id 24B9A8CEE68 11, 28 -- for {Microscopy-at-Microscopy.Com} ; Wed, 29 Oct 2008 10:51:49 -0700 (PDT) 11, 28 -- Received: from hlexc03.w2k.feico.com ([10.150.40.39]) by hlexc05.w2k.feico.com with Microsoft SMTPSVC(6.0.3790.3959); 11, 28 -- Wed, 29 Oct 2008 10:51:47 -0700 11, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 28 -- Content-class: urn:content-classes:message 11, 28 -- MIME-Version: 1.0 11, 28 -- Content-Type: text/plain; 11, 28 -- charset="us-ascii" 11, 28 -- Subject: Job at FEI Company supporting Titan TEM at University of Oregon 11, 28 -- Date: Wed, 29 Oct 2008 10:51:30 -0700 11, 28 -- Message-ID: {00948E5E66F1374FB9284A7B78138EB814C7E80C-at-hlexc03.w2k.feico.com} 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- Thread-Topic: Job at FEI Company supporting Titan TEM at University of Oregon 11, 28 -- Thread-Index: Ack57vll+v3dVM2kRQS7yXQ8swUh2w== 11, 28 -- From: "Resume" {Resume-at-fei.com} 11, 28 -- To: {Microscopy-at-Microscopy.Com} 11, 28 -- Cc: "Williamson, Joe" {Joe.Williamson-at-fei.com} 11, 28 -- X-OriginalArrivalTime: 29 Oct 2008 17:51:47.0842 (UTC) FILETIME=[03AD3620:01C939EF] 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9THpmCq003797 ==============================End of - Headers==============================
I've travel led to South Africa on an archaeological project with a view to study some material with my microscope. Unfortunately my microscope has been severely delayed and I am without a setup here.
I'm hoping that there may be somebody that has a lab in South Africa (South Coast near Cape Town) or has a contact in South Africa that would be willing to assist. I only need a simple reflected light microscope setup (200x) with digital imaging and/or an eyepiece reticule for measurement. Something like an Olympus BH2 would work wonders. I'm looking for help between now and my return to the UK at the end of November.
Any help would be appreciated
best
Adrian
-- ----------------------- Adrian A. Evans
Division of Archaeology, Geography, & Enivronmental Sciences School of Life Sciences University of Bradford BD7 1DP A.A.Evans-at-Bradford.ac.uk
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==============================Original Headers============================== 10, 24 -- From A.A.Evans-at-Bradford.ac.uk Wed Oct 29 13:35:12 2008 10, 24 -- Received: from hydrogen.cen.brad.ac.uk (hydrogen.cen.brad.ac.uk [143.53.238.3]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9TIZBbr019479 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 13:35:12 -0500 10, 24 -- Received: from radon.cen.brad.ac.uk (radon.cen.brad.ac.uk [143.53.238.18]) 10, 24 -- by hydrogen.cen.brad.ac.uk (8.13.6/8.13.4) with ESMTP id m9TIZAqW018714 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 18:35:11 GMT 10, 24 -- Received: from bradford.ac.uk (bismuth.cen.brad.ac.uk [143.53.238.79]) 10, 24 -- by radon.cen.brad.ac.uk (8.13.6/8.13.4) with ESMTP id m9TIZAVS007804 10, 24 -- for {microscopy-at-microscopy.com} ; Wed, 29 Oct 2008 18:35:10 GMT 10, 24 -- Received: from 41.247.17.115 ([41.247.17.115]) 10, 24 -- by webmail.brad.ac.uk (IMP) with HTTP 10, 24 -- for {aaevans-at-localhost} ; Wed, 29 Oct 2008 18:35:10 +0000 10, 24 -- Message-ID: {1225305310.4908acde2d5d9-at-webmail.brad.ac.uk} 10, 24 -- Date: Wed, 29 Oct 2008 18:35:10 +0000 10, 24 -- From: "Adrian A. Evans" {A.A.Evans-at-Bradford.ac.uk} 10, 24 -- To: microscopy-at-microscopy.com 10, 24 -- Subject: Urgent help required 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=ISO-8859-1 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 10, 24 -- X-UOBWebMail-Version: IMP3.2.3/TURBA1.2/HORDE2.2.5 10, 24 -- X-Originating-IP: 41.247.17.115 ==============================End of - Headers==============================
My take on this is that people need to collect the image that contains the data in the form they need. This doesn't always mean collecting the most "realistic" looking image, which is obvious if you're doing elemental mapping or hyperspectral imaging, etc, but not so obvious if you're looking at a stained section in brightfield or a whole specimen under a dissector. And doesn't necessarily mean collecting the most pixels with the most bit depth either. In my opinion, 95% of image processing/analysis is collecting the image data appropriately in the first place. This may need some trial/error at first, of course, figuring out what you and your analysis software need to discriminate treatment effects, distinguish the labelled component, etc.
(I'm of the generation that put a green filter in the light path of red-stained sections to enhance contrast on the black and white film....)
And you can always collect a separate image for the cover photo....
My 2c (or now only about 1c, esp. in Aussie money....) worth. cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334 ________________________________________ X-from: pekysar-at-ucdavis.edu [pekysar-at-ucdavis.edu] Sent: Thursday, 30 October 2008 2:01 a.m. To: White, Rosemary (PI, Black Mountain)
John, I think one of the primary goals is to instruct the students on use of the camera software. Nowadays the software out there is so dynamic that it can do tons of things, (i.e. montage etc). You might check with the manufacturers as they may have an educational edition so you can allow the students to use a modified version at their computer stations as a group. Also, since manipulation of digital images is limited, you might instruct them in what is acceptable for scientific images and what is not. Another item might be how to calculate and "burn in" micron markers so they are accurate for images which are cropped, enlarged etc. They may someday need to create images for a paper or poster. They need to know what are the limitations of resolutions for a poster? Paper? How to create insets? Students also need to learn how to make figure legends and how to label structures on their images neatly and accurately. A suggestion for a project might be to make a poster of their already saved images from other classes or taking one of their images, labeling the structures and putting the images on the same page as the figure legend--no cutting etc. Hope this helps! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {jkrupp-at-deltacollege.edu} To: {pekysar-at-ucdavis.edu} Sent: Tuesday, October 28, 2008 3:44 PM
Dear all,
We have got recently Baltec HPM 010 high-pressure freezer from the other lab. Unfortunately, after Baltec acquisition by Leica there is no service provided for old machines (Leica is pushing the new ones HPM100 and EMPACT2). Our machine has some faulty valve and we could not sort it out for some time. Does anybody know independent engineer/company in UK that can repair and service Baltec HPM 010?
Sincerely,
Dr. Aleksandr Mironov MD, PhD Experimental Officer D.1527, M.Smith Building EM Core Facility, Faculty of Life Sciences University of Manchester Oxford Road Manchester M13 9PT UK
We have a "fun" side project where we are trying to prepare thin sections through paper to examine the fibers/coatings and possible contaminants.
Does anyone have a good method for preparing thin sections? We have a microtome here as well as paraffin, epoxies, etc. Currently we have a problem with the fibers separating once the paraffin is treated with xylene. We presume the fibers may have to be fixed prior to embedding in paraffin.
Thanks.
Alan Stone
==============================Original Headers============================== 7, 19 -- From as-at-astonmet.com Thu Oct 30 13:07:17 2008 7, 19 -- Received: from outbound3.mail.tds.net (outbound3.mail.tds.net [216.170.230.93]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9UI7GK4005761 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 30 Oct 2008 13:07:17 -0500 7, 19 -- Received: from outaamta02.mail.tds.net (outaamta02.mail.tds.net [216.170.230.32]) 7, 19 -- by outbound3.mail.tds.net (8.13.6/8.13.4) with ESMTP id m9UI7GNM027260 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 30 Oct 2008 13:07:16 -0500 7, 19 -- Received: from mozart.astonmet.com ([69.11.219.4]) 7, 19 -- by outaamta02.mail.tds.net with ESMTP 7, 19 -- id {20081030180711.NOYF4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 30 Oct 2008 13:07:11 -0500 7, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 19 -- Date: Thu, 30 Oct 2008 13:07:10 -0500 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- From: Alan Stone {as-at-astonmet.com} 7, 19 -- Subject: Preparing Thin Sections of Paper 7, 19 -- Mime-Version: 1.0 7, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 19 -- Message-Id: {20081030180711.NOYF4416.outaamta02.mail.tds.net-at-mozart.astonmet.com} ==============================End of - Headers==============================
Dear Dr. Mironov, RMC is selling new high pressure freezers and as far as I know is doing service for the old Baltec ones.
Feel free to contact the nearest RMC dealer / service point to get more information: ISS Group Pellowe House, Francis Road Withington, Manchester M20 4XP, UK
Aleksandr.Mironov-at-manchester.ac.uk schrieb: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } We have got recently Baltec HPM 010 high-pressure freezer from the other } lab. Unfortunately, after Baltec acquisition by Leica there is no } service provided for old machines (Leica is pushing the new ones HPM100 } and EMPACT2). Our machine has some faulty valve and we could not sort it } out for some time. Does anybody know independent engineer/company in UK } that can repair and service Baltec HPM 010? } } Sincerely, } } Dr. Aleksandr Mironov MD, PhD } Experimental Officer } D.1527, M.Smith Building } EM Core Facility, Faculty of Life Sciences } University of Manchester } Oxford Road } Manchester } M13 9PT } UK } } Tel. +44-(0)161-275-5645 } Fax. +44-(0)161-275-5171 } E-mail: Aleksandr.Mironov-at-manchester.ac.uk } http://www.ls.manchester.ac.uk/research/facilities/electronmicroscopy/ } } } ==============================Original Headers============================== } 6, 28 -- From Aleksandr.Mironov-at-manchester.ac.uk Thu Oct 30 11:18:23 2008 } 6, 28 -- Received: from serenity.mcc.ac.uk (serenity.mcc.ac.uk [130.88.200.93]) } 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9UGINbS015200 } 6, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Oct 2008 11:18:23 -0500 } 6, 28 -- Received: from gomwe.mcc.ac.uk ([10.2.18.2]) } 6, 28 -- by serenity.mcc.ac.uk with esmtps (TLSv1:AES256-SHA:256) } 6, 28 -- (Exim 4.69 (FreeBSD)) } 6, 28 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) } 6, 28 -- id 1KvaE2-0000US-Et } 6, 28 -- for Microscopy-at-microscopy.com; Thu, 30 Oct 2008 16:18:22 +0000 } 6, 28 -- Received: from mi-amironov.smith.man.ac.uk ([130.88.210.224]:1027) } 6, 28 -- by gomwe.mcc.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) } 6, 28 -- (Exim 4.69 (FreeBSD)) } 6, 28 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) } 6, 28 -- id 1KvaE2-000ES5-Be } 6, 28 -- for Microscopy-at-microscopy.com; Thu, 30 Oct 2008 16:18:22 +0000 } 6, 28 -- Message-ID: {4909DE4F.2010302-at-manchester.ac.uk} } 6, 28 -- Date: Thu, 30 Oct 2008 16:18:23 +0000 } 6, 28 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} } 6, 28 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) } 6, 28 -- MIME-Version: 1.0 } 6, 28 -- To: Microscopy-at-microscopy.com } 6, 28 -- Subject: TEM: Baltec HPM 010 service in UK } 6, 28 -- Content-Type: text/plain; charset=windows-1251; format=flowed } 6, 28 -- Content-Transfer-Encoding: 7bit } 6, 28 -- X-Authenticated-Sender: Aleksandr Mironov from mi-amironov.smith.man.ac.uk [130.88.210.224]:1027 } 6, 28 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk } 6, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. } ==============================End of - Headers============================== }
As usual, I use a very straightforward way. Perhaps not the best, but it is so simple that it would be sad not at least to try it. I just grow cells on glass coverslips and glue the coverslips on alu stubs. You may buy special coverslips sizes, but I just break the glass. On a standard 18x18 you can grow thousands of cells! I doubt that anyone would take the time to analyse several thousand cells by SEM, so they are in excessive number anyway. Just make sure that you have a good contact with the alu stubs during the coating. ("flat" coating should work, but to make sure I usually coat with 3x120 degrees turning). Et voilà! Good luck.
Stephane
----- Original Message ---- X-from: "adler.jeremy-at-cellbio.su.se" {adler.jeremy-at-cellbio.su.se} To: nizets2-at-yahoo.com Sent: Tuesday, October 28, 2008 6:05:52 PM
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Email: adler.jeremy-at-cellbio.su.se Name: Jeremy Adler
Organization: Stockholm U
Title-Subject: [Filtered] Attaching cells to aluminium
Question: We want to examine cells in a dual beam SEM. One approach would be to grow cells on aluminium stubs. Any tips about the practicalities would be appreciated.
1) Advising to contact RMC representative in UK - ISS group 2) Bibst Labs are doing servicing for these machines in US and UK 3) Leica representative have contacted me saying that they are still servicing HPM010
Concerning the first advice I could say that ISS group was actually dealing and responsible for HPM010 movement from Paterson Cancer Institute to the University of Manchester and for reparation and recommissioning. However, they got some difficulties because of mentioned merger and they are still waiting for the engineers to be trained.
Thank you for all your replies!
Sincerely, Alex
==============================Original Headers============================== 6, 28 -- From Aleksandr.Mironov-at-manchester.ac.uk Fri Oct 31 11:07:39 2008 6, 28 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9VG7ce5032177 6, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 11:07:39 -0500 6, 28 -- Received: from gomwe.mcc.ac.uk ([10.2.18.2]) 6, 28 -- by probity.mcc.ac.uk with esmtps (TLSv1:AES256-SHA:256) 6, 28 -- (Exim 4.69 (FreeBSD)) 6, 28 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 6, 28 -- id 1KvwXC-0001RF-Cv 6, 28 -- for Microscopy-at-microscopy.com; Fri, 31 Oct 2008 16:07:38 +0000 6, 28 -- Received: from mi-amironov.smith.man.ac.uk ([130.88.210.224]:3668) 6, 28 -- by gomwe.mcc.ac.uk with esmtpsa (TLSv1:AES256-SHA:256) 6, 28 -- (Exim 4.69 (FreeBSD)) 6, 28 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 6, 28 -- id 1KvwXC-0004uq-83 6, 28 -- for Microscopy-at-microscopy.com; Fri, 31 Oct 2008 16:07:38 +0000 6, 28 -- Message-ID: {490B2D4C.6030703-at-manchester.ac.uk} 6, 28 -- Date: Fri, 31 Oct 2008 16:07:40 +0000 6, 28 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} 6, 28 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 6, 28 -- MIME-Version: 1.0 6, 28 -- To: Microscopy-at-microscopy.com 6, 28 -- Subject: Feed back (TEM: Baltec HPM 010 service in UK) 6, 28 -- Content-Type: text/plain; charset=windows-1251; format=flowed 6, 28 -- Content-Transfer-Encoding: 7bit 6, 28 -- X-Authenticated-Sender: Aleksandr Mironov from mi-amironov.smith.man.ac.uk [130.88.210.224]:3668 6, 28 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk 6, 28 -- X-UoM: Scanned by the University Mail System. See http://www.itservices.manchester.ac.uk/email/filtering/information/ for details. ==============================End of - Headers==============================
Referring back to the waving-chloroform-over-thin-sections thread, there were comments about using heat pens (mea culpa) and how they eat batteries. There is a simple solution to this problem: a 6V microscopy-lamp power supply. The kind used with separate lights on stereoscopes. Usually there are a few extras lurking in drawers and cabinets from old microscopes. Take about a meter (+/- by need) of lamp cord. On one end wire on the appropriate plu for the power supply, then at the other end, strip bare and solder the wires to the battery connectors in the heat pen. No more batteries needed, and you now have a varible-temperature (sort of) heat pen. We usually don't need more than 4V on the power supply selector, that's plenty enough to burn fingers or spread sections.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 2, 23 -- From oshel1pe-at-cmich.edu Fri Oct 31 12:18:33 2008 2, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9VHIXhQ017316 2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 12:18:33 -0500 2, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 2, 23 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id m9VHIUgB023581 2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 13:18:32 -0400 2, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 2, 23 -- Fri, 31 Oct 2008 13:17:49 -0400 2, 23 -- Mime-Version: 1.0 2, 23 -- Message-Id: {f06240805c530eca43763-at-[141.209.160.249]} 2, 23 -- Date: Fri, 31 Oct 2008 13:17:47 -0400 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 23 -- Subject: heat-pen redux 2, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 23 -- X-OriginalArrivalTime: 31 Oct 2008 17:17:49.0800 (UTC) FILETIME=[99BC2E80:01C93B7C] 2, 23 -- X-Canit-CHI2: 0.00 2, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 23 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 2, 23 -- X-CanItPRO-Stream: default 2, 23 -- X-Canit-Stats-ID: 4697953 - 6f6a8fe71747 2, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I have posted a couple images at http://www.emc.missouri.edu/lookatthis.htm showing some spherical fellows where they shouldn't be. We suspect some type of yeast, but if anyone has other thoughts on what they might be, a client would be ever so grateful to hear them.
Happy Halloween (and no, this is not a prank).
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 27 -- From TindallR-at-missouri.edu Fri Oct 31 12:36:41 2008 7, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9VHafex032279 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 12:36:41 -0500 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: ApoEAPneCknRauUo/2dsb2JhbADERgEJhX6EB4JWew 7, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 7, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 31 Oct 2008 12:36:40 -0500 7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 31 Oct 2008 12:36:40 -0500 7, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Subject: SEM: Odd beasties in bacterial culture 7, 27 -- Date: Fri, 31 Oct 2008 12:36:40 -0500 7, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7B02-at-UM-XMAIL08.um.umsystem.edu} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: SEM: Odd beasties in bacterial culture 7, 27 -- Thread-Index: Ack7fzsQygOfxW7KS/Ko6KLyRvBq9g== 7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 31 Oct 2008 17:36:40.0939 (UTC) FILETIME=[3BF24BB0:01C93B7F] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9VHafex032279 ==============================End of - Headers==============================
My apologies, but I forgot to indicate in my previous post that the images are of a bacterial culture. Long, thin things good. Roundish things bad.
Many thanks to those who have replied with ideas so far.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 27 -- From TindallR-at-missouri.edu Fri Oct 31 13:33:01 2008 7, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9VIX0jW015591 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 13:33:00 -0500 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: ApoEAMPrCknRauUo/2dsb2JhbADEZQEJhXiEB4JWew 7, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 7, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 31 Oct 2008 13:33:00 -0500 7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 31 Oct 2008 13:32:58 -0500 7, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Subject: SEM beasties 7, 27 -- Date: Fri, 31 Oct 2008 13:32:58 -0500 7, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7B09-at-UM-XMAIL08.um.umsystem.edu} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: SEM beasties 7, 27 -- Thread-Index: Ack7hxjuJbtuTnhpSOuBs29DUJ3lLw== 7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 31 Oct 2008 18:32:59.0132 (UTC) FILETIME=[1981A3C0:01C93B87] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9VIX0jW015591 ==============================End of - Headers==============================
Randy, Are the round things for sure critters? I've made similar looking round, clumpy stuff by precipitating inorganic materials to make fine powders. Is there anything in the medium that might precipitate for any reason?
Rob Bowen
Robert C. Bowen Research Scientist Caddock Electronics, Inc rob.bowen-at-caddock.com http://www.caddock.com
} From: {TindallR-at-missouri.edu} } Reply-To: {TindallR-at-missouri.edu} } Date: Fri, 31 Oct 2008 13:41:05 -0500 } To: {rob.bowen-at-caddock.com} } Subject: [Microscopy] SEM beasties } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Me, again. } } My apologies, but I forgot to indicate in my previous post that the } images are of a bacterial culture. Long, thin things good. Roundish } things bad. } } Many thanks to those who have replied with ideas so far. } } Randy } } Randy Tindall } Senior EM Specialist } Electron Microscopy Core Facility---We Do Small Well! } W125 Veterinary Medicine } University of Missouri } Columbia, MO 65211 } Tel: (573) 882-8304 } Fax: (573) 884-2227 } Email: tindallr-at-missouri.edu } Web: http://www.emc.missouri.edu } On-line calendar: } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= } Week&NavType=Both&Type=TimePlan } } } } ==============================Original Headers============================== } 7, 27 -- From TindallR-at-missouri.edu Fri Oct 31 13:33:01 2008 } 7, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu } (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) } 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m9VIX0jW015591 } 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 13:33:00 -0500 } 7, 27 -- X-IronPort-Anti-Spam-Filtered: true } 7, 27 -- X-IronPort-Anti-Spam-Result: } ApoEAMPrCknRauUo/2dsb2JhbADEZQEJhXiEB4JWew } 7, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) } ([209.106.229.40]) } 7, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 31 Oct 2008 } 13:33:00 -0500 } 7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by } um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 7, 27 -- Fri, 31 Oct 2008 13:32:58 -0500 } 7, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 7, 27 -- Content-class: urn:content-classes:message } 7, 27 -- MIME-Version: 1.0 } 7, 27 -- Content-Type: text/plain; } 7, 27 -- charset="us-ascii" } 7, 27 -- Subject: SEM beasties } 7, 27 -- Date: Fri, 31 Oct 2008 13:32:58 -0500 } 7, 27 -- Message-ID: } {91108EF9255B394CBF8B7E3789814A4103CD7B09-at-UM-XMAIL08.um.umsystem.edu} } 7, 27 -- X-MS-Has-Attach: } 7, 27 -- X-MS-TNEF-Correlator: } 7, 27 -- Thread-Topic: SEM beasties } 7, 27 -- Thread-Index: Ack7hxjuJbtuTnhpSOuBs29DUJ3lLw== } 7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} } 7, 27 -- To: {microscopy-at-microscopy.com} } 7, 27 -- X-OriginalArrivalTime: 31 Oct 2008 18:32:59.0132 (UTC) } FILETIME=[1981A3C0:01C93B87] } 7, 27 -- Content-Transfer-Encoding: 8bit } 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m9VIX0jW015591 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 21 -- From Rob.Bowen-at-caddock.com Fri Oct 31 15:22:44 2008 8, 21 -- Received: from msg.caddock.com (69-29-3-221.stat.centurytel.net [69.29.3.221]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9VKMhDM000981 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 15:22:43 -0500 8, 21 -- Received: from [10.1.2.107] ([10.1.2.107]) 8, 21 -- by msg.caddock.com (IceWarp 9.3.2) with ASMTP id LEB46644; 8, 21 -- Fri, 31 Oct 2008 13:22:44 -0700 8, 21 -- User-Agent: Microsoft-Entourage/11.3.3.061214 8, 21 -- Date: Fri, 31 Oct 2008 13:22:41 -0700 8, 21 -- Subject: Re: [Microscopy] SEM beasties 8, 21 -- From: Rob Bowen {Rob.Bowen-at-caddock.com} 8, 21 -- To: {TindallR-at-missouri.edu} 8, 21 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 21 -- Message-ID: {C530B721.86EF%Rob.Bowen-at-caddock.com} 8, 21 -- Thread-Topic: [Microscopy] SEM beasties 8, 21 -- Thread-Index: Ack7lmyaqzfsSKeJEd2IlQARJDSgFA== 8, 21 -- In-Reply-To: {200810311841.m9VIf5Nr025729-at-ns.microscopy.com} 8, 21 -- Mime-version: 1.0 8, 21 -- Content-type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
I want to make clear that the heat-pen/power supply kludge was originally done by my predecessor here, Geoff Williams. I just really like the idea and looked it over to see what he'd done. Something that's easy to duplicate. I would have named him originally but forgot in my enthusiasm. Sorry to Geoff and the list.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 23 -- From oshel1pe-at-cmich.edu Mon Nov 3 08:44:58 2008 3, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3Eiw0J019045 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 08:44:58 -0600 3, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 23 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mA3Eivtk004988 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 09:44:58 -0500 3, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 23 -- Mon, 3 Nov 2008 09:44:15 -0500 3, 23 -- Mime-Version: 1.0 3, 23 -- Message-Id: {f06240813c534bdfecb2c-at-[141.209.160.249]} 3, 23 -- Date: Mon, 3 Nov 2008 09:44:12 -0500 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 23 -- Subject: heat pen follow-up 3, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 23 -- X-OriginalArrivalTime: 03 Nov 2008 14:44:15.0595 (UTC) FILETIME=[A4E153B0:01C93DC2] 3, 23 -- X-Canit-CHI2: 0.00 3, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 23 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 3, 23 -- X-CanItPRO-Stream: default 3, 23 -- X-Canit-Stats-ID: 4767205 - 8b7b04511363 3, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I think it depends on how hot the pen is -- an advantage of using a variable power supply -- and simply that no one method works for spreading all resins. We have the best luck with Spurr's, which resin are you using?
Phil
} I would like to weigh in on this issue. I bought a heat pen and did not have } much success with spreading out my thick (one micron) samples. I found it } tedious to get the sections to flatten out. I have to admit, I } resorted back to } my Q-tip dipped in chloroform. Can anyone elaborate on my problems? } (I bought } the PELCO model and it was not cheap!) } } Thanks! } Peggy } } -----Original Message----- } From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: Monday, November 03, 2008 9:56 AM } To: Sherwood, Margaret } Subject: [Microscopy] heat pen follow-up } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 3, 27 -- From oshel1pe-at-cmich.edu Mon Nov 3 10:31:48 2008 3, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3GVmlv018044 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 10:31:48 -0600 3, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mA3GVbMF018117 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 11:31:47 -0500 3, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 27 -- Mon, 3 Nov 2008 11:31:40 -0500 3, 27 -- Mime-Version: 1.0 3, 27 -- Message-Id: {f06240817c534d76bc0bf-at-[141.209.160.249]} 3, 27 -- In-Reply-To: 3, 27 -- {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C919-at-PHSXMB30.partners.org} 3, 27 -- References: {200811031455.mA3Etxq2000627-at-ns.microscopy.com} 3, 27 -- {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C919-at-PHSXMB30.partners.org} 3, 27 -- Date: Mon, 3 Nov 2008 11:31:37 -0500 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 27 -- Subject: RE: [Microscopy] heat pen follow-up 3, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 27 -- X-OriginalArrivalTime: 03 Nov 2008 16:31:40.0282 (UTC) FILETIME=[A63685A0:01C93DD1] 3, 27 -- X-Canit-CHI2: 0.00 3, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 27 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 3, 27 -- X-CanItPRO-Stream: default 3, 27 -- X-Canit-Stats-ID: 4774822 - 7ab23abdb347 3, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
We use the Pelco pen with great luck on Epon thin sections but had only modest success with 0.5-1.0 micron thick sections. It does slightly better for butyl, methyl-methacrylate sections. I have had no luck with the battery powered versions. I probably will try to the design of Geoff Williams that Phil described just for kicks. I knew I saved all those all lamps for something.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Monday, November 03, 2008 10:33 AM To: Phillips, Thomas E.
I think it depends on how hot the pen is -- an advantage of using a variable power supply -- and simply that no one method works for spreading all resins. We have the best luck with Spurr's, which resin are you using?
Phil
} I would like to weigh in on this issue. I bought a heat pen and did not have } much success with spreading out my thick (one micron) samples. I found it } tedious to get the sections to flatten out. I have to admit, I } resorted back to } my Q-tip dipped in chloroform. Can anyone elaborate on my problems? } (I bought } the PELCO model and it was not cheap!) } } Thanks! } Peggy } } -----Original Message----- } From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: Monday, November 03, 2008 9:56 AM } To: Sherwood, Margaret } Subject: [Microscopy] heat pen follow-up } } } } } ----------------------------------------------------------------------- ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 3, 27 -- From oshel1pe-at-cmich.edu Mon Nov 3 10:31:48 2008 3, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3GVmlv018044 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 10:31:48 -0600 3, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mA3GVbMF018117 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 11:31:47 -0500 3, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 27 -- Mon, 3 Nov 2008 11:31:40 -0500 3, 27 -- Mime-Version: 1.0 3, 27 -- Message-Id: {f06240817c534d76bc0bf-at-[141.209.160.249]} 3, 27 -- In-Reply-To: 3, 27 -- {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C919-at-PHSXMB30.partners.org} 3, 27 -- References: {200811031455.mA3Etxq2000627-at-ns.microscopy.com} 3, 27 -- {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C919-at-PHSXMB30.partners.org} 3, 27 -- Date: Mon, 3 Nov 2008 11:31:37 -0500 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 27 -- Subject: RE: [Microscopy] heat pen follow-up 3, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 27 -- X-OriginalArrivalTime: 03 Nov 2008 16:31:40.0282 (UTC) FILETIME=[A63685A0:01C93DD1] 3, 27 -- X-Canit-CHI2: 0.00 3, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 27 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 3, 27 -- X-CanItPRO-Stream: default 3, 27 -- X-Canit-Stats-ID: 4774822 - 7ab23abdb347 3, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 29 -- From PhillipsT-at-missouri.edu Mon Nov 3 11:11:43 2008 13, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3HBhIZ005947 13, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 11:11:43 -0600 13, 29 -- X-IronPort-Anti-Spam-Filtered: true 13, 29 -- X-IronPort-Anti-Spam-Result: ApoEAHW/DknRauUo/2dsb2JhbADBKgEJhQ+EHwGCVns 13, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 13, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 03 Nov 2008 11:11:42 -0600 13, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 13, 29 -- Mon, 3 Nov 2008 11:11:41 -0600 13, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 29 -- Content-class: urn:content-classes:message 13, 29 -- MIME-Version: 1.0 13, 29 -- Content-Type: text/plain; 13, 29 -- charset="US-ASCII" 13, 29 -- Subject: RE: [Microscopy] RE: heat pen follow-up 13, 29 -- Date: Mon, 3 Nov 2008 11:11:39 -0600 13, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05376A26-at-UM-XMAIL06.um.umsystem.edu} 13, 29 -- In-Reply-To: {200811031632.mA3GWZbT019273-at-ns.microscopy.com} 13, 29 -- X-MS-Has-Attach: 13, 29 -- X-MS-TNEF-Correlator: 13, 29 -- Thread-Topic: [Microscopy] RE: heat pen follow-up 13, 29 -- thread-index: Ack90cekCyQPb7ihQUGo4Xn2lFicCwAA0weA 13, 29 -- References: {200811031632.mA3GWZbT019273-at-ns.microscopy.com} 13, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 13, 29 -- To: {Microscopy-at-microscopy.com} 13, 29 -- X-OriginalArrivalTime: 03 Nov 2008 17:11:41.0488 (UTC) FILETIME=[3D717B00:01C93DD7] 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3HBhIZ005947 ==============================End of - Headers==============================
The Digital Micrograph (DM) we use for our microscope has stopped communication with the digital camera on our TEM. In depth details below if you are able to help.
We have a Gatan multiscan camera which communicates with a FasTEM control on a JEOL JEM-2011. The Gatan camera is connected to the DM computer, which runs on Windows XP, through a firewire card.
The problem started with an error for when clicking 'start view' on the camera floating window. It read something like "image could not be collected as being viewed by another process", so I tried a restart of the program. The DM program now has a greyed out camera menu, as well as a greyed out floating window for the camera and digiscan. I am using DM ver 1.8.
I have tried reinstalling DM 1.8, an earlier version of DM (1.7), reinstalling the drivers of the firewire card, reinstalling the driver for the FasTEM communication as well as system restores (no problems detected in device manager for drivers and there is power from the firewire card), all without success. I have also tried disconnecting the firewire cable of the digiscan incase there was a power distribution problem.
Does anyone have any suggestions about anything else I could try, or if anyone has encountered problem similar before?
Any help gratefully received,
Calum
==============================Original Headers============================== 9, 25 -- From Calum.Dickinson-at-staffmail.ul.ie Mon Nov 3 11:58:43 2008 9, 25 -- Received: from Marshal4.ul.ie (marshal4.ul.ie [193.1.100.137]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3Hwekb026459 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 11:58:43 -0600 9, 25 -- Received: from staffexchange1.ul.campus (Not Verified[193.1.101.31]) by Marshal4.ul.ie with MailMarshal (v6,4,5,5695) 9, 25 -- id {B490f3bcf0000} ; Mon, 03 Nov 2008 17:58:39 +0000 9, 25 -- Received: from staffexchange5.ul.campus ([10.220.1.55]) by staffexchange1.ul.campus with Microsoft SMTPSVC(6.0.3790.3959); 9, 25 -- Mon, 3 Nov 2008 17:58:39 +0000 9, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 25 -- Content-class: urn:content-classes:message 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; 9, 25 -- charset="iso-8859-1" 9, 25 -- Subject: TEM: Digital Micrograph camera communication problem 9, 25 -- Date: Mon, 3 Nov 2008 17:58:39 -0000 9, 25 -- Message-ID: {27E2EA9EA725D6438C8F9B6F0B57A82D05366E56-at-staffexchange5.ul.campus} 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- Thread-Topic: TEM: Digital Micrograph camera communication problem 9, 25 -- Thread-Index: Ack93cz0YcsqaEA+QrutpaZv87FyJQ== 9, 25 -- From: "Calum.Dickinson" {calum.dickinson-at-ul.ie} 9, 25 -- To: {microscopy-at-microscopy.com} 9, 25 -- X-OriginalArrivalTime: 03 Nov 2008 17:58:39.0112 (UTC) FILETIME=[CCE0C880:01C93DDD] 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3Hwekb026459 ==============================End of - Headers==============================
I would normally warm up thick or semi-thick sections (~1um and thicker) on a drop of water on a glass slide using a hot plate or slide warming plate. You can leave the sections for much longer to spread out. Perhaps not so easy if the sections are on a grid - but possible I'm sure.
Malcolm
Malcolm Haswell Electron Microscope Unit The Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: oshel1pe-at-cmich.edu
Greetings all, Sara asked me to post the following for her:
Dear microscopy students and advisors,
I would like to tell you about upcoming educational opportunities for faculty and students. There are two very exciting meetings coming up soon that not only are exceptional scientific presentations, but also sponsor student participation.
First, the Microscopy and Microanalysis conference (July 26-30, 2009, Richmond, VA) is the largest microscopy meeting with the largest trade show floor in the world. The exhibits occupy over 100,000 sq ft with instrumentation including light, laser, transmission electron and scanning electron microscopes, as well as ancillary equipment, and book vendors.
Furthermore, the Microscopy Society of America has thirteen $1000-fellowships for students (various ones for undergraduate, graduate, medical resident, postdoctoral) to present their work. The awardees are chosen based on use of any kind of microscopy (e.g., light, fluorescence, confocal, electron, ion, Xray, etc.) and scientific merit. Our sister society the Microbeam Analysis Society also gives six student awards of $600 each. Other scientific and service awards are also presented. Abstracts for M&M 2009 are due February 15, 2009. More information is available at the MSA web site: http://www.microscopy.org (click on Meeting/Conferences then Microscopy & Microanalysis 2009, then Award Competitions) or go directly to the M&M 2009 web site http://microscopy.org/MMMeetings/MM09 . MAS awards are described at http://www.microbeam analysis.org/index.htm (click Awards).
Richmond is centrally located within 350 miles of half the population of the US-reasonably easy to reach without an air ticket. Also, the city is serviced by 7 major airlines, and the airport is only about 8 miles away from downtown. Hotel accommodations are reasonable, and student housing will be available at the university for a greatly reduced rate. Additional assistance is available for students who help staff the meeting (contact Amanda Lawrence alawrence-at-emcenter.msstate.edu).
Secondly, the Society for Ultrastructural Pathology, an international organization of doctors and scientists who study disease at the fine structural level will hold its meeting in the same city in July, 2010. Student competition is also encouraged for those who have investigated ultrastructure, either in the research or clinical setting. Attendees do not have to be pathologists to participate. A Pathologist-in Training Award ($1000) is made to the resident with the best presentation. More information will follow as details become available.
I urge you to make these meetings known to your faculty and students so that they can be preparing talks. We welcome their participation and student competition. The student does not have to be a member of either society to compete, but will be encouraged to join at a reduced rate if interested.
Feel free to call or email me if you have any questions.
Very sincerely yours, Sara Miller, a past president of MSA
Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710
Apparently some people have not received the newsletter / meeting announcement that was mailed on October 15th. The purpose of that newsletter was to announce our fall meeting to be held Tuesday, November 18, 2008 at the Doubletree Hotel in Carson, CA.
If you have not received that newsletter, please accept our apologies. Details of the meeting can be found on the SCSMM website http://www.scsmm.org/NextMeet.htm
Sincerely,
Your SCSMM Board
==============================Original Headers============================== 8, 19 -- From bozhilov-at-ucr.edu Mon Nov 3 13:00:32 2008 8, 19 -- Received: from sentoku.ucr.edu (sentoku.ucr.edu [138.23.226.244]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3J0WPc003175 8, 19 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 13:00:32 -0600 8, 19 -- Received: from igppb116g.ucr.edu (igppb116g.ucr.edu [138.23.185.162]) 8, 19 -- by sentoku.ucr.edu (MOS 3.10.3-GA) 8, 19 -- with ESMTP id DIO60915 (AUTH bozhilov-at-ucr.edu) 8, 19 -- for {microscopy-at-microscopy.com} ; 8, 19 -- Mon, 3 Nov 2008 11:00:31 -0800 (PST) 8, 19 -- Message-Id: {A64B0701-8BDA-4ECF-8EF4-FBFD577601B0-at-ucr.edu} 8, 19 -- From: "K.N. Bozhilov" {bozhilov-at-ucr.edu} 8, 19 -- To: microscopy-at-microscopy.com 8, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit 8, 19 -- Mime-Version: 1.0 (Apple Message framework v929.2) 8, 19 -- Subject: Nov. 18, 2008 meeting Carson, CA 8, 19 -- Date: Mon, 3 Nov 2008 11:00:31 -0800 8, 19 -- X-Mailer: Apple Mail (2.929.2) 8, 19 -- X-Junkmail-Status: score=0/50, host= ==============================End of - Headers==============================
Try swapping the Firewire controller for the camera and your Digiscan. We have had Firewire controllers just go bad from age and need replacement. Swapping them will help diagnose if you have a bad one.
John Mardinly, Numonyx
-----Original Message----- X-from: calum.dickinson-at-ul.ie [mailto:calum.dickinson-at-ul.ie] Sent: Monday, November 03, 2008 10:06 AM To: MARDINLY, A
Dear anyone that can help,
The Digital Micrograph (DM) we use for our microscope has stopped communication with the digital camera on our TEM. In depth details below if you are able to help.
We have a Gatan multiscan camera which communicates with a FasTEM control on a JEOL JEM-2011. The Gatan camera is connected to the DM computer, which runs on Windows XP, through a firewire card.
The problem started with an error for when clicking 'start view' on the camera floating window. It read something like "image could not be collected as being viewed by another process", so I tried a restart of the program. The DM program now has a greyed out camera menu, as well as a greyed out floating window for the camera and digiscan. I am using DM ver 1.8.
I have tried reinstalling DM 1.8, an earlier version of DM (1.7), reinstalling the drivers of the firewire card, reinstalling the driver for the FasTEM communication as well as system restores (no problems detected in device manager for drivers and there is power from the firewire card), all without success. I have also tried disconnecting the firewire cable of the digiscan incase there was a power distribution problem.
Does anyone have any suggestions about anything else I could try, or if anyone has encountered problem similar before?
Any help gratefully received,
Calum
==============================Original Headers============================== 9, 25 -- From Calum.Dickinson-at-staffmail.ul.ie Mon Nov 3 11:58:43 2008 9, 25 -- Received: from Marshal4.ul.ie (marshal4.ul.ie [193.1.100.137]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3Hwekb026459 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 11:58:43 -0600 9, 25 -- Received: from staffexchange1.ul.campus (Not Verified[193.1.101.31]) by Marshal4.ul.ie with MailMarshal (v6,4,5,5695) 9, 25 -- id {B490f3bcf0000} ; Mon, 03 Nov 2008 17:58:39 +0000 9, 25 -- Received: from staffexchange5.ul.campus ([10.220.1.55]) by staffexchange1.ul.campus with Microsoft SMTPSVC(6.0.3790.3959); 9, 25 -- Mon, 3 Nov 2008 17:58:39 +0000 9, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 25 -- Content-class: urn:content-classes:message 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; 9, 25 -- charset="iso-8859-1" 9, 25 -- Subject: TEM: Digital Micrograph camera communication problem 9, 25 -- Date: Mon, 3 Nov 2008 17:58:39 -0000 9, 25 -- Message-ID: {27E2EA9EA725D6438C8F9B6F0B57A82D05366E56-at-staffexchange5.ul.campus} 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- Thread-Topic: TEM: Digital Micrograph camera communication problem 9, 25 -- Thread-Index: Ack93cz0YcsqaEA+QrutpaZv87FyJQ== 9, 25 -- From: "Calum.Dickinson" {calum.dickinson-at-ul.ie} 9, 25 -- To: {microscopy-at-microscopy.com} 9, 25 -- X-OriginalArrivalTime: 03 Nov 2008 17:58:39.0112 (UTC) FILETIME=[CCE0C880:01C93DDD] 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3Hwekb026459 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 29 -- From A.MARDINLY-at-numonyx.com Mon Nov 3 14:01:20 2008 18, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3K1KJB019133 18, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 3 Nov 2008 14:01:20 -0600 18, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 18, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 194684143A6; 18, 29 -- Mon, 3 Nov 2008 14:43:56 -0500 (EST) 18, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 18, 29 -- Mon, 3 Nov 2008 15:01:19 -0500 18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 29 -- Content-class: urn:content-classes:message 18, 29 -- MIME-Version: 1.0 18, 29 -- Content-Type: text/plain; 18, 29 -- charset="us-ascii" 18, 29 -- Subject: RE: [Microscopy] TEM: Digital Micrograph camera communication problem 18, 29 -- Date: Mon, 3 Nov 2008 15:01:16 -0500 18, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9A8A73E-at-EXDRESBENMX012.numonyx.local} 18, 29 -- In-Reply-To: {200811031805.mA3I5kge005946-at-ns.microscopy.com} 18, 29 -- X-MS-Has-Attach: 18, 29 -- X-MS-TNEF-Correlator: 18, 29 -- Thread-Topic: [Microscopy] TEM: Digital Micrograph camera communication problem 18, 29 -- Thread-Index: Ack93uIzUppaDVmBTsOGk8D3GVhwnAAD6eLw 18, 29 -- References: {200811031805.mA3I5kge005946-at-ns.microscopy.com} 18, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 18, 29 -- To: {calum.dickinson-at-ul.ie} 18, 29 -- Cc: {Microscopy-at-Microscopy.com} 18, 29 -- X-OriginalArrivalTime: 03 Nov 2008 20:01:19.0936 (UTC) FILETIME=[F0456400:01C93DEE] 18, 29 -- Content-Transfer-Encoding: 8bit 18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3K1KJB019133 ==============================End of - Headers==============================
Did you install Service Pack 3 for Windows XP? We found that we had to uninstall this feature for our firewire cameras to work. This was a fix for a different vendor's camera and may not be the solution.
Mike
------------------------------------------------------------------------ ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear anyone that can help,
The Digital Micrograph (DM) we use for our microscope has stopped communication with the digital camera on our TEM. In depth details below if you are able to help.
We have a Gatan multiscan camera which communicates with a FasTEM control on a JEOL JEM-2011. The Gatan camera is connected to the DM computer, which runs on Windows XP, through a firewire card.
The problem started with an error for when clicking 'start view' on the camera floating window. It read something like "image could not be collected as being viewed by another process", so I tried a restart of the program. The DM program now has a greyed out camera menu, as well as a greyed out floating window for the camera and digiscan. I am using DM ver 1.8.
I have tried reinstalling DM 1.8, an earlier version of DM (1.7), reinstalling the drivers of the firewire card, reinstalling the driver for the FasTEM communication as well as system restores (no problems detected in device manager for drivers and there is power from the firewire card), all without success. I have also tried disconnecting the firewire cable of the digiscan incase there was a power distribution problem.
Does anyone have any suggestions about anything else I could try, or if anyone has encountered problem similar before?
Any help gratefully received,
Calum
==============================Original Headers============================== 9, 25 -- From Calum.Dickinson-at-staffmail.ul.ie Mon Nov 3 11:58:43 2008 9, 25 -- Received: from Marshal4.ul.ie (marshal4.ul.ie [193.1.100.137]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3Hwekb026459 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 11:58:43 -0600 9, 25 -- Received: from staffexchange1.ul.campus (Not Verified[193.1.101.31]) by Marshal4.ul.ie with MailMarshal (v6,4,5,5695) 9, 25 -- id {B490f3bcf0000} ; Mon, 03 Nov 2008 17:58:39 +0000 9, 25 -- Received: from staffexchange5.ul.campus ([10.220.1.55]) by staffexchange1.ul.campus with Microsoft SMTPSVC(6.0.3790.3959); 9, 25 -- Mon, 3 Nov 2008 17:58:39 +0000 9, 25 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 25 -- Content-class: urn:content-classes:message 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain; 9, 25 -- charset="iso-8859-1" 9, 25 -- Subject: TEM: Digital Micrograph camera communication problem 9, 25 -- Date: Mon, 3 Nov 2008 17:58:39 -0000 9, 25 -- Message-ID: {27E2EA9EA725D6438C8F9B6F0B57A82D05366E56-at-staffexchange5.ul.campus} 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- Thread-Topic: TEM: Digital Micrograph camera communication problem 9, 25 -- Thread-Index: Ack93cz0YcsqaEA+QrutpaZv87FyJQ== 9, 25 -- From: "Calum.Dickinson" {calum.dickinson-at-ul.ie} 9, 25 -- To: {microscopy-at-microscopy.com} 9, 25 -- X-OriginalArrivalTime: 03 Nov 2008 17:58:39.0112 (UTC) FILETIME=[CCE0C880:01C93DDD] 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3Hwekb026459 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 29 -- From A.MARDINLY-at-numonyx.com Mon Nov 3 14:01:20 2008 18, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3K1KJB019133 18, 29 -- for {Microscopy-at-Microscopy.com} ; Mon, 3 Nov 2008 14:01:20 -0600 18, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 18, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 194684143A6; 18, 29 -- Mon, 3 Nov 2008 14:43:56 -0500 (EST) 18, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 18, 29 -- Mon, 3 Nov 2008 15:01:19 -0500 18, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 29 -- Content-class: urn:content-classes:message 18, 29 -- MIME-Version: 1.0 18, 29 -- Content-Type: text/plain; 18, 29 -- charset="us-ascii" 18, 29 -- Subject: RE: [Microscopy] TEM: Digital Micrograph camera communication problem 18, 29 -- Date: Mon, 3 Nov 2008 15:01:16 -0500 18, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9A8A73E-at-EXDRESBENMX012.numonyx.local} 18, 29 -- In-Reply-To: {200811031805.mA3I5kge005946-at-ns.microscopy.com} 18, 29 -- X-MS-Has-Attach: 18, 29 -- X-MS-TNEF-Correlator: 18, 29 -- Thread-Topic: [Microscopy] TEM: Digital Micrograph camera communication problem 18, 29 -- Thread-Index: Ack93uIzUppaDVmBTsOGk8D3GVhwnAAD6eLw 18, 29 -- References: {200811031805.mA3I5kge005946-at-ns.microscopy.com} 18, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 18, 29 -- To: {calum.dickinson-at-ul.ie} 18, 29 -- Cc: {Microscopy-at-Microscopy.com} 18, 29 -- X-OriginalArrivalTime: 03 Nov 2008 20:01:19.0936 (UTC) FILETIME=[F0456400:01C93DEE] 18, 29 -- Content-Transfer-Encoding: 8bit 18, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3K1KJB019133 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 23 -- From mdunlap-at-ucmerced.edu Mon Nov 3 15:40:03 2008 17, 23 -- Received: from mario.ucmerced.edu (mario.ucmerced.edu [169.236.10.223]) 17, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA3Le27C003568 17, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 15:40:03 -0600 17, 23 -- Received: from mdunlap-laptop ([169.236.142.218]) 17, 23 -- (authenticated bits=0) 17, 23 -- by mario.ucmerced.edu (8.12.10/8.12.9) with ESMTP id mA3Le1Mw011745 17, 23 -- (version=TLSv1/SSLv3 cipher=EDH-RSA-DES-CBC3-SHA bits=168 verify=NOT) 17, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Nov 2008 13:40:02 -0800 (PST) 17, 23 -- From: "Michael Dunlap" {mdunlap-at-ucmerced.edu} 17, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 23 -- Subject: RE: TEM: Digital Micrograph camera communication problem 17, 23 -- Date: Mon, 3 Nov 2008 13:39:52 -0800 17, 23 -- Thread-Index: Ack9/LCo3in6VFsYRb+VQnVeHKtcTg== 17, 23 -- Message-ID: {20081103133952916.00000001336-at-mdunlap-laptop} 17, 23 -- In-Reply-To: {200811032003.mA3K3xrG024190-at-ns.microscopy.com} 17, 23 -- References: {200811032003.mA3K3xrG024190-at-ns.microscopy.com} 17, 23 -- X-Mailer: Oracle Connector for Outlook 10.1.3.0.10 100801 (11.0.8217) 17, 23 -- X-Accept-Language: en-us, en 17, 23 -- MIME-Version: 1.0 17, 23 -- Content-Type: text/plain; charset=us-ascii 17, 23 -- Content-Transfer-Encoding: 8bit 17, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA3Le27C003568 ==============================End of - Headers==============================
=} life expectancy of cathode (standard tungsten cathode) ? trouble due to steel Wehnelt-cylinder ? =} ZEISS EM109 transmission electron microscope with transfiber optics and external roll film camera
Colleagues, due intermittent spark over at 80 kV, I switched before one year from my old Wehnelt cylinder (= Wehnelt-head) made from ALUMINIUM (but massively chromated) to a new one made from stainless steel. in the steel Wehnelt cylinder (no more spark over as with the aluminium one at 80 kV) the first cathode worked for 14 hours, the second one for 39 hours, but the next three cathodes (all bought from Zeiss) all died already during careful heating up, i.e they died within 10-15 SECONDS. All parts of the Wehnelt-cylinder were cleaned before inserting the new cathode; position & heighth of cathode-tip within Wehnelt aperture seemed to me "as ever". In my desperation I put back a new cathode into the old (uncleaned) Aluminium-Wehnelt cylinder and its running smoothely for hours now.
Has anybody made similar experiences and can give some advice? thanks, peter heimann
Are you really meaning that Bacillus anthracis is good whereas Saccharomyces cerevisiae is bad? We don't have the same values for these tiny things! ;-)
BTW, I would be glad if you could share your preparation method with me.
Regards,
Stephane
----- Original Message ---- X-from: "TindallR-at-missouri.edu" {TindallR-at-missouri.edu} To: nizets2-at-yahoo.com Sent: Friday, October 31, 2008 7:38:35 PM
Me, again.
My apologies, but I forgot to indicate in my previous post that the images are of a bacterial culture. Long, thin things good. Roundish things bad.
Many thanks to those who have replied with ideas so far.
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
==============================Original Headers============================== 7, 27 -- From TindallR-at-missouri.edu Fri Oct 31 13:33:01 2008 7, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m9VIX0jW015591 7, 27 -- for {microscopy-at-microscopy.com} ; Fri, 31 Oct 2008 13:33:00 -0500 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: ApoEAMPrCknRauUo/2dsb2JhbADEZQEJhXiEB4JWew 7, 27 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 7, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 31 Oct 2008 13:33:00 -0500 7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Fri, 31 Oct 2008 13:32:58 -0500 7, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="us-ascii" 7, 27 -- Subject: SEM beasties 7, 27 -- Date: Fri, 31 Oct 2008 13:32:58 -0500 7, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7B09-at-UM-XMAIL08.um.umsystem.edu} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: SEM beasties 7, 27 -- Thread-Index: Ack7hxjuJbtuTnhpSOuBs29DUJ3lLw== 7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 31 Oct 2008 18:32:59.0132 (UTC) FILETIME=[1981A3C0:01C93B87] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m9VIX0jW015591 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 22 -- From nizets2-at-yahoo.com Tue Nov 4 06:00:31 2008 23, 22 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 23, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mA4C0VrG003695 23, 22 -- for {microscopy-at-microscopy.com} ; Tue, 4 Nov 2008 06:00:31 -0600 23, 22 -- Received: (qmail 71619 invoked by uid 60001); 4 Nov 2008 12:00:31 -0000 23, 22 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 22 -- s=s1024; d=yahoo.com; 23, 22 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 23, 22 -- b=NIvAgpWeB8t58cxs2wzz8j02yzY6yiRAHtOBl8tPc29iUhluupcmlVyfZ0/GAj8OMS4d1SMnP0XfM4Mc53Y+MTUbrW1Ifx9ig0hm+AIp7HNNVJFXtlzb1aLj2LbUJpAcfB31qiw5a3Kkz5EDIihnBmdy5IgcIsvnrnMQhyWJmDw=; 23, 22 -- X-YMail-OSG: 4gHanCMVM1lAR_W2FNlC9IT.NL1_O_9ZAGCLlmKUvrZDPrsO8_H0Ggp6WV8cl6yDDeYjerjwdXD2EbPmhe0imzlmaN_EogVJR1e6J0XZwropFdvUMWhcSXgXPg3xL.i4JP4oHM9AXWbLwyJreJUb9q_blO_.2C3DqJsihrZos6EaS4kF9eukducXrofxdqOrnpeQ5WKItY4lTk84w0Zv26sK24U32rlAbdxy6ZVMZdBs2MpFnD0vyfw1tQgoC2bQrmdR837zbE0qfA-- 23, 22 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Tue, 04 Nov 2008 04:00:30 PST 23, 22 -- X-Mailer: YahooMailRC/1155.20 YahooMailWebService/0.7.247.3 23, 22 -- Date: Tue, 4 Nov 2008 04:00:30 -0800 (PST) 23, 22 -- From: Stephane Nizet {nizets2-at-yahoo.com} 23, 22 -- Subject: Re: [Microscopy] SEM beasties 23, 22 -- To: TindallR-at-missouri.edu 23, 22 -- Cc: microscopy-at-microscopy.com 23, 22 -- MIME-Version: 1.0 23, 22 -- Content-Type: text/plain; charset=iso-8859-1 23, 22 -- Message-ID: {968945.71541.qm-at-web37402.mail.mud.yahoo.com} 23, 22 -- Content-Transfer-Encoding: 8bit 23, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA4C0VrG003695 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both therese_ellefsen-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: student, Oslo National Academy Of The Arts
Title-Subject: [Filtered] micro-chemical visualisation of lethal drugs
Question: What substanses can be used with these chemicals: Potassium Chloride Pancuronium Bromide and Sodium Thiopenthal, to make a micro-chemical grafic/visualisation?. And what kind of photo equipment can be used in this prosess?
I am a master student at Oslo National Academy Of The Arts, in Norway and i am working on project about the death penalty. The reason why i need those images is because i intend to make several oil paintings, using the photographs as models.
I am in contact with a professor at the Norwegian School of veterinary Science, Adrian Smith, that has these chemicals. But he does not seem to know how to make these photo micrographs. He is supposed to find a chemist that can make these pictures for me, but from the mails i have reseived it seems like he has not talked to a chemist since he doesnt understand how or what to do. So, since i have to start work with the artistic side of my project, i cant put my time in other peoples hands and wait. Thats why i am posting these questions, so that i know what can be used in making these images and give that information to the veterinary school and see if that takes me further.
I might have to rethink the visual side of my project, but i realy think that the visual side of these images would be interesting for my project. So, thank you again for answering my mail, and hope someone can answer my question.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bill.mcmanus-at-usu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bill.mcmanus-at-usu.edu Name: Bill McManus
Organization: Western Dairy Center, Utah State University
Title-Subject: [Filtered] what's new in immuno-labeling?
Question: I am getting back into doing immuno-labeling at the SEm and Confocal level. I was wondering if there have been any signicant advances in the last six years?
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Email: susan.vanhorn-at-sunysb.edu Name: Susan C. Van Horn
Organization: StonyBrook Univ
Title-Subject: [Filtered] myelin artifact
Question: I am embedding perfused (2-4% Para/2.5% glut in either 0.1M PBS or sod. cacodylate buffer pH7.4) cerrebellum, corpus callossum and spinal cord. I am using 2% osmium tet. and am embedding in either Spurr or Durcupan resin. I am seeing within the layers of the myelin sheath, sparatic accumulation of some type of electron dense material....any ideas on what is causing this not so pretty artifact??? - thanks so much!! Sue
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Question: Hi Fellow Listers, I'm trying to get our first SEM approved through budget this year. My application is drug particles, excipients and the like. (All non-conducting materials.) I've been told I should also get a sputter coater to help make life easier and was wondering if there are any recommendations for ones you like in particular. Ease of use is a priority, but low cost is also a good thing. Thanks in advance, Heather
Here is the November 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Friday November 7, 2008. Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor
===========================
STORM Offers Super-Resolution in 3D! Stephen W. Carmichael, Mayo Clinic
Imaging Multilayers of Ice with Scanning Tunneling Microscopy Konrad Thürmer and Norman C. Bartelt, Sandia National Laboratories, Livermore, CA
Atom-Probe Tomography – Different Analysis Tools for Three-Dimensional Atomic-Resolution Data Robert M. Ulfig, David J. Larson, David A. Reinhard, Thomas F. Kelly, Imago Scientific Instruments Corporation, Madison WI
Application of the Gatan X-ray Ultramicroscope (XuM) to the Investigation of Material and Biological Samples Paul Mainwaring, Gatan, Inc., Pleasanton, CA
Characterization of Surface and Sub-Surface Defects on Devices using Complimentary Techniques Vincent S. Smentkowski, Sara G. Ostrowski, Lauraine Denault, Charles G. Woychik, GE-Global Research, Niskayuna NY
Novel Life Science Tensile Stage Integration with Cryo Dual-Beam FIB Technology Debra M. Sherman, Life Science Microscopy Facility, Purdue Univ., W. Lafayette, IN
Ultrafast Confocal Raman Imaging H. Fischer, T. Dieing, O. Hollricher, WITec GmbH, Ulm, Germany
Breaking the Resolution Barrier in the Scanning Electron Microscope W. Vanderlinde, Lab. for Physical Sciences, College Park, MD
Improving Image Quality and Reducing Drift Problems via Automated Data Acquisition and Averaging in a Cs-Corrected TEM E. Voelkl,1 B. Jiang,1,2 Z.R. Dai,2 and J.P Bradley,2;1FEI Company, Hillsboro, OR, 2 Livermore Natl. Lab., Livermore, CA
Single Cell Force Measurements and Cell Adhesion T. Mueller and T. Neumann, JPK Instruments AG, Berlin Germany
The McCrone Atlas of Microscopic Particles: The Modern Dynamic Particle Reference Resource David A. Wiley, McCrone Associates, Westmont, IL
A cryoSEM Method for Preservation and Visualization of Calcified Shark Cartilage M. N. Dean1, S. N. Gorb2 & A. P. Summers1; 1U. California, Irvine, CA, 2Max Planck Inst., for Metals Res., Stuttgart, Germany
The Use of Poly-L-lysine as an Adhesive in Scanning Electron Microscopy Jeannette Taylor, Emory University, Atlanta, GA
MSA Local Affiliate Soc., New England Soc. for Microscopy
Preparing Micrographs and Applying Scale Bars Using Adobe Photoshop Elements™ Susan A. Lancelle, Mount Holyoke College, South Hadley, MA
Nannobacteria, Organic Matter, and Precipitation in Hot Springs, Viterbo, Italy: Distinctions and Relevance B. L. Kirkland, F. L. Lynch, R. L. Folk*, A.M. Lawrence, and M.E. Corley, Miss. State U., Mississippi State, MS, U. of Texas, Austin, TX
Industry News
NetNotes SPECIMEN PREPARATION - Negative staining cyanobacteria SPECIMEN PREPARTION - Aclar film FREEZE-SUBSTITION - water in acetone MICROTOMY- vibration dampening TEM - high resolution mode TEM - Increasing contrast EM – water chillers EM - pH in recirculators ESEM validation EDX – how does it work? EM - Imaging Tantalum particles MICROSCOPY – resolution limits INSTRUMENTATION – sputter coater troubleshooting SPECIMEN PREPARATION – room temperature embedding SPECIMEN PREPARATION – mineralized bone SPECIMEN PREPARTION – tray for small items Dear Abbe
Advertiser's Index
==============================Original Headers============================== 27, 17 -- From randerson20-at-tampabay.rr.com Tue Nov 4 08:27:15 2008 27, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.124]) 27, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA4ERE7f032415 27, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Nov 2008 08:27:15 -0600 27, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 27, 17 -- by hrndva-omta01.mail.rr.com with ESMTP 27, 17 -- id {20081104142713.ZCA27474.hrndva-omta01.mail.rr.com-at-[127.0.0.1]} 27, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 4 Nov 2008 14:27:13 +0000 27, 17 -- Message-ID: {49105BB7.3090105-at-tampabay.rr.com} 27, 17 -- Date: Tue, 04 Nov 2008 09:27:03 -0500 27, 17 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 27, 17 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 27, 17 -- MIME-Version: 1.0 27, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 27, 17 -- Subject: [Fwd: November 2008 Microscopy Today Table of Contents] 27, 17 -- Content-Type: text/plain; charset=windows-1252; format=flowed 27, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
X-from memory and things I've heard, my vote is a thumbs up to Denton models and a thumbs down to Hummer models.
Stu Smalinskas, P.E. SKF Plymouth, Michigan
} } Email: heather.eberhardt-at-frx.com } Name: Heather Eberhardt } } Organization: Forest Research Institute } } Title-Subject: [Filtered] Sputter Coaters } } Question: Hi Fellow Listers, } I'm trying to get our first SEM approved through budget } this year. } My application is drug particles, excipients and the like. } (All } non-conducting materials.) I've been told I should } also get a } sputter coater to help make life easier and was wondering } if there } are any recommendations for ones you like in particular. } Ease of use } is a priority, but low cost is also a good thing. } Thanks in advance, } Heather }
==============================Original Headers============================== 6, 21 -- From smalinskas-at-yahoo.com Tue Nov 4 09:03:11 2008 6, 21 -- Received: from web34405.mail.mud.yahoo.com (web34405.mail.mud.yahoo.com [66.163.178.154]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mA4F3AT5025903 6, 21 -- for {microscopy-at-sparc5.microscopy.com} ; Tue, 4 Nov 2008 09:03:10 -0600 6, 21 -- Received: (qmail 16277 invoked by uid 60001); 4 Nov 2008 15:03:10 -0000 6, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 21 -- s=s1024; d=yahoo.com; 6, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Message-ID; 6, 21 -- b=JS1LJjwwoVpEGTp+nJMBGdTxvYS6m72k6rcHEJoJCp70KeN1/RVNsFwSDfThfBS1qMaBtdNu5p5p6oEzrDylRndBVp+kJ0yKfL6qE1kWSwRKL1ikOIJuxXCCOMtm1Ru7IVzwNPTOqTV+ubMpWZNly8O2Xj6ac4CQw/bbaGHwfWE=; 6, 21 -- X-YMail-OSG: 0IjLTA8VM1nnc.gQoWp2jJVG2cBOafrq74MfuBQPS7F30B5vIp_vI_c7N8uw7TNvsJFZp_QoEzzv.VNaLt7hpPvSkpruOd4ERXBpOW4BrnNnnMvAbxxQg0HAcU2j9GlsuTmJMpouOoVWQhW0AwoiIPtm4PBCY8WLVcMM5pZWno_fI8kCHDdvYnJ87yg- 6, 21 -- Received: from [141.151.33.213] by web34405.mail.mud.yahoo.com via HTTP; Tue, 04 Nov 2008 07:03:10 PST 6, 21 -- X-Mailer: YahooMailWebService/0.7.260.1 6, 21 -- Date: Tue, 4 Nov 2008 07:03:10 -0800 (PST) 6, 21 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 6, 21 -- Reply-To: smalinskas-at-yahoo.com 6, 21 -- Subject: Re: [Microscopy] viaWWW: Sputter Coaters 6, 21 -- To: heather.eberhardt-at-frx.com, microscopy-at-ns.microscopy.com 6, 21 -- In-Reply-To: {200811041427.mA4ERL3w032574-at-ns.microscopy.com} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; charset=us-ascii 6, 21 -- Message-ID: {381455.15743.qm-at-web34405.mail.mud.yahoo.com} ==============================End of - Headers==============================
Heather, If you can get an FE-SEM approved for your use you will not need a sputter coater or any sort. Purchasing anything but an FE-SEM would be a waste of time and money. A conventional tungsten source, even with an environmental mode, would not only require a sputter coater, and the additional cost of the proper sputtering source, but countless hours of experimenting with the correct settings with which to LIGHTLY coat your samples. If your coating becomes too thick, the morphology of your sample is lost. If you are going to get x-ray added to your SEM system, that is even more reason to not coat. The silicon-drift detectors get great signal even from a 10-microamp beam on an FE-SEM. In addition, the newest version of Hitachi's S-4800-II FE-SEM has CSS or Charge Suppression Scan which is amazing. I saw the charging, an hence image distortion, simply disappear while imaging an Al2O3 nodule. I am not sure if any other manufacturers have this feature.
Good luck,
Bob Passeri
-----Original Message----- X-from: heather.eberhardt-at-frx.com [mailto:heather.eberhardt-at-frx.com] Sent: Tuesday, November 04, 2008 8:32 AM To: bobpasseri-at-ameritech.net
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both heather.eberhardt-at-frx.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hi Fellow Listers, I'm trying to get our first SEM approved through budget this year. My application is drug particles, excipients and the like. (All non-conducting materials.) I've been told I should also get a sputter coater to help make life easier and was wondering if there are any recommendations for ones you like in particular. Ease of use is a priority, but low cost is also a good thing. Thanks in advance, Heather
I like FE-SEMs. In fact, we are in the market for one for our lab. However, I take exception with Bob's opinion that anything beside an FE-SEM would be a waste of money.
We have two tungsten-gun SEMs that we have used to produce a lot of results over the years. They have been real workhorses. One is a conventional high-vacuum SEM. The other is a variable-pressure model that is used in VP-mode about 90% of the time on uncoated samples. But now it is time for a new scope and we are pushing the resolution limits on our existing scopes. We think we need to make the jump to a field-emission gun.
However, there is a reason that not every microscope sold has a field-emission gun. There is a substantial cost premium over the cost of an SEM with a tungsten-gun. We could probably find the funds to purchase another conventional SEM, but we need to find someone with much deeper pockets if we are going to purchase a FE-SEM.
I hope you have already done some soul searching to determine what kind of scope you really need. Many samples in our lab do not require the brightness or resolution of an FE-gun. Sure it would be nice, but is it justifiable? Maybe there is no important microstructure above 5000x. Low voltage examination is good, but not always essential. Our older EDS detector is overwhelmed with more than 5000 cps. So why do I really need an FE-gun?
Maybe you have money to throw away in this economy. We sure don't.
So back to sputter coaters... We have used Polaron and Denton coaters with success for many years. Our models could use some more automation, but most of our students can figure them out well enough. If they can't run a coater, I don't want them touching an SEM.
I would look at options for rotating and tilting the samples to get a good coating on all sides. That is our biggest concern about our present equipment.
You might also look at options for metals beside Au or Pt or Pd. I understand Cr is often used for high resolution work, but it takes a different setup than the other metals. It is probably only needed if you are getting an FE-SEM, and if you are, I concur with Bob that you may be able to use other techniques such as low voltage or VP-mode to eliminate the need for coating. But then again, if you have the budget for an FE-SEM, you probably have the budget for a Cr coater.
I hope this helps.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: bobpasseri-at-ameritech.net [mailto:bobpasseri-at-ameritech.net] Sent: Tuesday, November 04, 2008 9:52 AM To: wesaia-at-iastate.edu
Heather, If you can get an FE-SEM approved for your use you will not need a sputter coater or any sort. Purchasing anything but an FE-SEM would be a waste of time and money. A conventional tungsten source, even with an environmental mode, would not only require a sputter coater, and the additional cost of the proper sputtering source, but countless hours of experimenting with the correct settings with which to LIGHTLY coat your samples. If your coating becomes too thick, the morphology of your sample is lost. If you are going to get x-ray added to your SEM system, that is even more reason to not coat. The silicon-drift detectors get great signal even from a 10-microamp beam on an FE-SEM. In addition, the newest version of Hitachi's S-4800-II FE-SEM has CSS or Charge Suppression Scan which is amazing. I saw the charging, an hence image distortion, simply disappear while imaging an Al2O3 nodule. I am not sure if any other manufacturers have this feature.
Good luck,
Bob Passeri
-----Original Message----- X-from: heather.eberhardt-at-frx.com [mailto:heather.eberhardt-at-frx.com] Sent: Tuesday, November 04, 2008 8:32 AM To: bobpasseri-at-ameritech.net
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both heather.eberhardt-at-frx.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Question: Hi Fellow Listers, I'm trying to get our first SEM approved through budget this year. My application is drug particles, excipients and the like. (All non-conducting materials.) I've been told I should also get a sputter coater to help make life easier and was wondering if there are any recommendations for ones you like in particular. Ease of use is a priority, but low cost is also a good thing. Thanks in advance, Heather
Heather, While I share Bob Passeri's enthusiasm for the Field Emission gun, I think you will benefit from a sputter coater nevertheless. In my experience, sputter coating is useful not only to solve charging problems but to gain contrast. I have samples that can be imaged without charging on the new generation FESEMs but without anywhere near the contrast that we get by adding a light coat of platinum. Of course, working without a coat is great when you can, but there are still samples where you cannot.
In terms of coaters, I don't have a good feel for pros and cons of different makers. I will add that I have had nice results over the years with platinum coats. We had a chromium target and it was a disaster -- the Cr coat oxidizes within in minutes to an insulating oxide. In my understanding, the only way to take advantage of the finer grain with Cr is to have a transfer device so you can coat and load the sample under vacuum the whole time.
Good luck in in your search,
Tobias
} ------------------------------------ } } Heather, } If you can get an FE-SEM approved for your use you will not need a } sputter coater or any sort. Purchasing anything but an FE-SEM would be a } waste of time and money. A conventional tungsten source, even with an } environmental mode, would not only require a sputter coater, and the } additional cost of the proper sputtering source, but countless hours of } experimenting with the correct settings with which to LIGHTLY coat your } samples. If your coating becomes too thick, the morphology of your sample } is lost. If you are going to get x-ray added to your SEM system, that is } even more reason to not coat. The silicon-drift detectors get great signal } even from a 10-microamp beam on an FE-SEM. } In addition, the newest version of Hitachi's S-4800-II FE-SEM has } CSS or Charge Suppression Scan which is amazing. I saw the charging, an } hence image distortion, simply disappear while imaging an Al2O3 nodule. I } am not sure if any other manufacturers have this feature. } } Good luck, } } Bob Passeri } } -----Original Message----- } X-from: heather.eberhardt-at-frx.com [mailto:heather.eberhardt-at-frx.com] } Sent: Tuesday, November 04, 2008 8:32 AM } To: bobpasseri-at-ameritech.net } Subject: [Microscopy] viaWWW: Sputter Coaters } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
There is some interaction of a decision based on what type of SEM you would get relative to the coater. But it seems that if you are looking at pharmaceutical materials and particles, a W or preferably LaB6 SEM ought to be satisfactory. Probably you will be in the {30KX mag range. A LaB6 should give plenty of brightness and be able to support EDS, which you will likely need as well. A 10mm^2 SDD ought to fit nicely, last a long time and require minimal maintenance.
So, for coating... If {30KX mag, one does not need exotic coating techniques. The challenge IMO is to use the right coating material. You will have big challenges at light element values ( {5KeV) and the coating should not get in the way of other elements that are of interest for EDS. Depending on what your specimens are, I have found that there is not one material for all cases. The best IMO are Pd and Ir. Au is too coarse even for low mag. Au/Pd would work but puts low eV peaks where they are not welcome. Pd is good since it is generally out of the way and is easily discernable with its characteristic twin peaks.
To coat, I'd suggest looking at the Denton Desk IV. It has two flavors. One is basic with an oil diaphragm pump. The other is TSC with molecular drag pump. For low mag work ( {80KX sort of), the turbo is not necessary, IMO. Even so, if a turbo is used, the oil pump should be replaced with an Edwards dry scroll pump. The real hassle of hydrocarbon polymerization is exacerbated by any oil-based pumps--those in the SEM as well. So, when looking at SEMs, see if you can get one with a specimen interchange load lock. This keeps the chamber always under vacuum and greatly reduces contamination of the chamber. A turbo pump versus oil diffusion pump is also a good option along with a scroll roughing pump. This is not a good idea if specimens are changed through the chamber door since the pump down time will be compromised...depending of course on the size/volume of the chamber. the goal is to reduce contamination without resorting to any LN2.
Finally, since you are not looking at ultra small particles, coating can be easily done at about 30mT, 50% power and 20% tilt. Time will vary depending on the specimen and which target material you use.
Hope this helps...if not too much confusing. Take specimens to vendor demo sites, for sure.
gary g.
At 06:27 AM 11/4/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Tue Nov 4 18:54:50 2008 9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mA50snjw001610 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 4 Nov 2008 18:54:49 -0600 9, 20 -- Message-Id: {200811050054.mA50snjw001610-at-ns.microscopy.com} 9, 20 -- Received: (qmail 24558 invoked from network); 4 Nov 2008 16:45:34 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 24554, pid: 24556, t: 0.1332s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by smtp2 with SMTP; 4 Nov 2008 16:45:34 -0800 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Tue, 04 Nov 2008 16:54:45 -0800 9, 20 -- To: heather.eberhardt-at-frx.com 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: Sputter Coaters 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200811041427.mA4ERYRb000929-at-ns.microscopy.com} 9, 20 -- References: {200811041427.mA4ERYRb000929-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I think that most SEM microscopists know that SBT manufacturers the IBS/e which is a high end sputter coater, but I'm not addressing that. I would like to address the comment made by Tobias with respect to Cr. Yes Cr does oxidize rapidly, but not in a matter of minutes. It takes somewhat longer than that, but it is a problem that needs to be addressed when using Cr. When ion sputtered, Cr is a very good and arguably the best coating for high resolution SEM coating. There are some considerations when using it. You have to first get rid of the oxide coating on the target by sputtering it first in vacuum immediately before coating your sample, otherwise you will introduce an oxide coating on your sample immediately. Once that is gone, you can sputter coat your sample. When you remove the sample from the coater, as Tobias says, it is best to image the sample right away. If you can't do that or you want to save the sample for further examination, we have introduced the SampleSaver(TM) storage container that stores and transports the sample in a purged inert atmosphere such as N2 or Ar. Cr coated samples was one of the reasons for the development of this product. The positive pressure inert gas is better than a vacuum pumped container for several reasons. The first is that you can not pump a small container to a sufficiently high vacuum through small tubing to get rid of oxidants, primarily water vapor. The second is that most inexpensive vacuum containers will allow some atmospheric components through the container or seals by diffusion. Another very good reason is that a suitable vacuum pump is not always available when you are transporting a sample because they can be kind of heavy to carry along in your sample basket. The SampleSaver(TM) storage containers have successfully stored samples of Ag without oxidation for as long as a month without problems. They work very well with Cr and Cu samples too. Ir and W coatings have been used for high resolution coatings to substitute for Cr because they don't oxidize as readily. Over time, there will still be an oxide layer on these coatings as well.
In short, if the proper precautions are taken Cr and other coatings that are susceptible to oxidation can be used as a coating and the samples saved even after viewing for a very long time.
Disclaimer: SBT manufactures and sells the IBS/e ion beam sputter coater system and the SampleSaver(TM) storage container.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Tuesday, November 04, 2008 3:38 PM To: Walck-at-SouthBayTech.com
Heather, While I share Bob Passeri's enthusiasm for the Field Emission gun, I think you will benefit from a sputter coater nevertheless. In my experience, sputter coating is useful not only to solve charging problems but to gain contrast. I have samples that can be imaged without charging on the new generation FESEMs but without anywhere near the contrast that we get by adding a light coat of platinum. Of course, working without a coat is great when you can, but there are still samples where you cannot.
In terms of coaters, I don't have a good feel for pros and cons of different makers. I will add that I have had nice results over the years with platinum coats. We had a chromium target and it was a disaster -- the Cr coat oxidizes within in minutes to an insulating oxide. In my understanding, the only way to take advantage of the finer grain with Cr is to have a transfer device so you can coat and load the sample under vacuum the whole time.
Good luck in in your search,
Tobias
} ------------------------------------ } } Heather, } If you can get an FE-SEM approved for your use you will not need a } sputter coater or any sort. Purchasing anything but an FE-SEM would be } a waste of time and money. A conventional tungsten source, even with } an environmental mode, would not only require a sputter coater, and the } additional cost of the proper sputtering source, but countless hours of } experimenting with the correct settings with which to LIGHTLY coat your } samples. If your coating becomes too thick, the morphology of your } sample is lost. If you are going to get x-ray added to your SEM } system, that is even more reason to not coat. The silicon-drift } detectors get great signal even from a 10-microamp beam on an FE-SEM. } In addition, the newest version of Hitachi's S-4800-II FE-SEM has CSS } or Charge Suppression Scan which is amazing. I saw the charging, an } hence image distortion, simply disappear while imaging an Al2O3 nodule. } I am not sure if any other manufacturers have this feature. } } Good luck, } } Bob Passeri } } -----Original Message----- } X-from: heather.eberhardt-at-frx.com [mailto:heather.eberhardt-at-frx.com] } Sent: Tuesday, November 04, 2008 8:32 AM } To: bobpasseri-at-ameritech.net } Subject: [Microscopy] viaWWW: Sputter Coaters } } ----------------------------------------------------------------------- } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
But you mention only Saccharomyces cerevisiae. Some of us would argue that Saccharomyces bayanus is more important. O:-)
paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 6, 23 -- From paul_hazelton-at-umanitoba.ca Tue Nov 4 23:01:48 2008 6, 23 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA551mi3001124 6, 23 -- for {microscopy-at-microscopy.com} ; Tue, 4 Nov 2008 23:01:48 -0600 6, 23 -- Received: from [192.168.100.102] (wnpgmb014uw-ad04-37-236.dynamic.mts.net [207.161.37.236]) 6, 23 -- (authenticated bits=0) 6, 23 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id mA551WDe025534 6, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 23 -- Tue, 4 Nov 2008 23:01:46 -0600 (CST) 6, 23 -- Message-ID: {491128AF.8090503-at-umanitoba.ca} 6, 23 -- Date: Tue, 04 Nov 2008 23:01:35 -0600 6, 23 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 6, 23 -- Organization: University of Manitoba 6, 23 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 6, 23 -- MIME-Version: 1.0 6, 23 -- To: nizets2-at-yahoo.com 6, 23 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 6, 23 -- Subject: Re: [Microscopy] Re: SEM beasties 6, 23 -- References: {200811041201.mA4C1jI4006575-at-ns.microscopy.com} 6, 23 -- In-Reply-To: {200811041201.mA4C1jI4006575-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
} I would just like to thank everyone that emailed me with suggestions on how I could fix this problem. } } Fortunately it is now back up and running after I switched the cables around from the multiscan and digiscan. Don't ask me how, although one is a gold coated cable, or why but it has seemed to do the trick at least for the short term. Whether this was purely coincidence, but it might be the firewire card that is playing up that some people suggested so I may buy another to replace just in case. } } Anyway, thanks again for the level of response. } } Calum
==============================Original Headers============================== 3, 26 -- From Calum.Dickinson-at-staffmail.ul.ie Wed Nov 5 03:02:43 2008 3, 26 -- Received: from Marshal4.ul.ie (marshal4.ul.ie [193.1.100.137]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA592gvU022144 3, 26 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 03:02:42 -0600 3, 26 -- Received: from staffexchange1.ul.campus (Not Verified[193.1.101.31]) by Marshal4.ul.ie with MailMarshal (v6,4,5,5695) 3, 26 -- id {B4911612f0000} ; Wed, 05 Nov 2008 09:02:39 +0000 3, 26 -- Received: from staffexchange5.ul.campus ([10.220.1.55]) by staffexchange1.ul.campus with Microsoft SMTPSVC(6.0.3790.3959); 3, 26 -- Wed, 5 Nov 2008 09:02:39 +0000 3, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 3, 26 -- Content-class: urn:content-classes:message 3, 26 -- MIME-Version: 1.0 3, 26 -- Content-Type: text/plain; 3, 26 -- charset="iso-8859-1" 3, 26 -- Subject: TEM: Digital Micrograph camera problem-solved 3, 26 -- Date: Wed, 5 Nov 2008 09:02:39 -0000 3, 26 -- Message-ID: {27E2EA9EA725D6438C8F9B6F0B57A82D05366E7D-at-staffexchange5.ul.campus} 3, 26 -- In-Reply-To: {27E2EA9EA725D6438C8F9B6F0B57A82D05366E72-at-staffexchange5.ul.campus} 3, 26 -- X-MS-Has-Attach: 3, 26 -- X-MS-TNEF-Correlator: 3, 26 -- Thread-Topic: TEM: Digital Micrograph camera problem-solved?!?! 3, 26 -- Thread-Index: Ack+mC7//UZQklFCT92UMzUtZmqkEwAjPIMA 3, 26 -- From: "Calum.Dickinson" {calum.dickinson-at-ul.ie} 3, 26 -- To: {microscopy-at-microscopy.com} 3, 26 -- X-OriginalArrivalTime: 05 Nov 2008 09:02:39.0550 (UTC) FILETIME=[411C8DE0:01C93F25] 3, 26 -- Content-Transfer-Encoding: 8bit 3, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA592gvU022144 ==============================End of - Headers==============================
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Email: p.lemoine-at-ulster.ac.uk Name: patrick lemoine
Organization: university of ulster
Title-Subject: [Filtered] stem vesrus tem
Question: As I have no direct experience with either stm or tem,I would apreciate comments on the following;
1/ Resolution is better with TEM but atomic res is still easily obtainable with STEM, as long it is not 1 angstrom res you are looking for (structure of carbon nanotube or graphite for instance is easily seen with STEM)
2/ STEM is much more user friendly than TEM, especially for people who have experience in using SEM
3/ STEM does not require as extensive sample thinning as TEM, TEM needs wafer always thinner than 100nm, STEM wafers can be 1micron (but not for ato. Res, obviously)
4/ STEM allows more analysis to be done because you don't have the lens stage below the sample, so it is easier to do EELS, electron diffraction, EDX, ect.
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Email: pekysar-at-ucdavis.edu Name: Pat Kysar
Organization: University of California, Davis
Title-Subject: [Filtered] Processing Arabidopsis Seeds for TEM
Question: Hello Listers, I have a researcher who wants to look at thin sections of Arabidopsis seeds in the TEM. He is particularly interested in the embryos and the lipid layer. The problem is that I have not been able to process them adequately. There are holes in the sections and the middle of the seeds are not fixed. I have been fixing them in 2% paraformaldehyde + 2.5% Glutaraldehyde; post fixed in reduced osmium; dehydrated with acetone and infiltrated and embedded in Epon/Araldite mixture. I use vacuum during fixation and infiltration and have extended the infiltration steps considerably (24 hours each step). From the white material I seen in some (not all) of the seeds, my guess is that the osmium is not getting past the seed coat. Does anyone have some tips I can try on these little buggers? I know there have been some threads on processing seeds but I have not been successful in locating them in the archives. Any help would be greatly appreciated.
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Email: mike.flaws-at-canterbury.ac.nz Name: Mike Flaws
Organization: University of Canterbury
Title-Subject: [Filtered] SF6 Detector necessary?
Question: Hi, Ý I am about to install a TEM that has SF6 in the HT tank. I have read that an SF6 detector should be used to continuously monitor the air in the room for leaks. Is this commonly done or is the likelihood of a leak so remote that it is unnecessary? Ý There is a big difference in the price of a detector that monitors continuously vs one that is used for occasional use - such as when the filament is replaced. Ý Thanks in advance for your help. Ý Mike
Energy Beam Sciences Inc. is a distributor of the Polaron, and Emitech lines of SEM preparation equipment, including sputter coaters.
Coaters come in a wide range of sizes and functions, from mini coaters that utilize noble metals for basic SEM coating, to large chamber, high vacuum systems that work with oxidizing and other specific metals.
You may contact EBS, with specific questions, on the technical, and functional aspects on sputter coaters, and good luck on your search for a coater, as there are many good options you have to choose from.
Mike Dufraine EM-Product Manager 800-992-9037 X340
heather.eberhardt-at-frx.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both heather.eberhardt-at-frx.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: heather.eberhardt-at-frx.com } Name: Heather Eberhardt } } Organization: Forest Research Institute } } Title-Subject: [Filtered] Sputter Coaters } } Question: Hi Fellow Listers, } I'm trying to get our first SEM approved through budget this year. } My application is drug particles, excipients and the like. (All } non-conducting materials.) I've been told I should also get a } sputter coater to help make life easier and was wondering if there } are any recommendations for ones you like in particular. Ease of use } is a priority, but low cost is also a good thing. } Thanks in advance, } Heather } } Login Host: 69.27.233.254 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 11 -- From zaluzec-at-microscopy.com Tue Nov 4 08:26:03 2008 } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA4EQ2jk030293 } 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 4 Nov 2008 08:26:03 -0600 } 6, 11 -- Mime-Version: 1.0 } 6, 11 -- Message-Id: {p06240809c5360be08c50-at-[206.69.208.22]} } 6, 11 -- Date: Tue, 4 Nov 2008 08:26:02 -0600 } 6, 11 -- To: microscopy-at-microscopy.com } 6, 11 -- From: heather.eberhardt-at-frx.com (by way of MicroscopyListserver) } 6, 11 -- Subject: viaWWW: Sputter Coaters } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 9, 21 -- From mdufraine-at-ebsciences.com Wed Nov 5 08:47:40 2008 9, 21 -- Received: from mail.ebsciences.com (mail.ebsciences.com [65.115.7.18]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA5EldmL031203 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 08:47:39 -0600 9, 21 -- Received: from mdufraine.ebsciences.private ([10.10.0.112]) 9, 21 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 9, 21 -- (Exim 4.69) 9, 21 -- (envelope-from {mdufraine-at-ebsciences.com} ) 9, 21 -- id 1KxjfW-0000UR-Um; Wed, 05 Nov 2008 09:47:38 -0500 9, 21 -- Message-ID: {4911B20B.6040706-at-ebsciences.com} 9, 21 -- Date: Wed, 05 Nov 2008 09:47:39 -0500 9, 21 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 9, 21 -- Organization: Energy Beam Sciences 9, 21 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 9, 21 -- MIME-Version: 1.0 9, 21 -- To: heather.eberhardt-at-frx.com, microscopy-at-microscopy.com 9, 21 -- Subject: Re: [Microscopy] viaWWW: Sputter Coaters 9, 21 -- References: {200811041426.mA4EQcMH031301-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200811041426.mA4EQcMH031301-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would like to get some suggestions and comments in choosing a digital camera between Olympus Morada and Gatan 832 SC1000 for our Tecnai-12 biotwin TEM. The TEM is for both biological and material imaging needs. Maximum magnification is 300K though most users won't need more than 200K.
Thank you for all you suggestions and comments. Ping
==============================Original Headers============================== 5, 22 -- From pli-at-dal.ca Wed Nov 5 12:21:01 2008 5, 22 -- Received: from sxt-sm-1.ucis.dal.ca (smtp.Dal.Ca [129.173.5.72]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA5IL0D6023161 5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 12:21:01 -0600 5, 22 -- Received: from SISPL (PLi-7.Biology.Dal.Ca [129.173.34.142]) 5, 22 -- by sxt-sm-1.ucis.dal.ca (8.14.2+Sun/8.14.2) with SMTP id mA5IKKvf026543 5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 14:20:21 -0400 (AST) 5, 22 -- Message-ID: {EEBA38C4CC314EABBE260CB9D0C0F700-at-SISPL} 5, 22 -- From: "Ping Li" {pli-at-dal.ca} 5, 22 -- To: {microscopy-at-microscopy.com} 5, 22 -- Subject: TEM camera: Olympus Morada vs. Gatan 832 SC1000 5, 22 -- Date: Wed, 5 Nov 2008 14:20:28 -0400 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; 5, 22 -- format=flowed; 5, 22 -- charset="gb2312"; 5, 22 -- reply-type=response 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Priority: 3 5, 22 -- X-MSMail-Priority: Normal 5, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 5, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Mike; A lot depends on the size of the room and the air circulation that you have and local regulations. SF6 is more of an asphyxiant than a toxin, and since it is quite heavy, any leaks would accumulate from the floor and not be breathed until the room filled up to the height of your head. You need to find out the volume of the SF6 in your tank and do a simple calculation to see how far it would rise in your room. We have two HV systems, one of which developed a slow leak after ~12 years (the only effect was that we could not turn on the HT until it was re-charged) and the other that has not leaked in 11 years. We have an oxygen sensor in one lab that our safety people insisted on for nitrogen from the EDX system, but it is ~5 feet off the ground and would not detect an SF6 leak. The old system once required service, and the SF6 was removed by venting into trash bags and carrying the trash bags outside. It only took a few bags to complete the task.
John Mardinly, Numonyx
-----Original Message----- X-from: mike.flaws-at-canterbury.ac.nz [mailto:mike.flaws-at-canterbury.ac.nz] Sent: Wednesday, November 05, 2008 5:45 AM To: MARDINLY, A
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Email: mike.flaws-at-canterbury.ac.nz Name: Mike Flaws
Organization: University of Canterbury
Title-Subject: [Filtered] SF6 Detector necessary?
Question: Hi, Ý I am about to install a TEM that has SF6 in the HT tank. I have read that an SF6 detector should be used to continuously monitor the air in the room for leaks. Is this commonly done or is the likelihood of a leak so remote that it is unnecessary? Ý There is a big difference in the price of a detector that monitors continuously vs one that is used for occasional use - such as when the filament is replaced. Ý Thanks in advance for your help. Ý Mike
On Nov 5, 2008, at 5:34 AM, pekysar-at-ucdavis.edu wrote:
} Does anyone have some tips I can try on these little buggers? I know } there have been some threads on processing seeds but I have not been } successful in locating them in the archives. Any help would be } greatly appreciated.
Dear Pat, I have had only very limited experience examining seeds, but preparation is definitely the key. The person who embedded and sectioned the seeds also found that the knife would often pull the seeds out of the resin, leaving holes. We did have some success by altering the composition of the resin until there was a good match of the hardness of the resin with that of the seeds (germinating pine, in this case). You will have to experiment with compositions until you find what works for your specimen, and, since I was doing microanalysis, I didn't want to stain the seeds, so the composition that worked for us is not necessarily going to work for you (even if I could remember what it was). Good luck. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 23 -- From tivol-at-caltech.edu Wed Nov 5 13:44:00 2008 6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA5Ji0DC020395 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 13:44:00 -0600 6, 23 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 23 -- by fire-doxen-postvirus (Postfix) with ESMTP id 05B32328BFD; 6, 23 -- Wed, 5 Nov 2008 11:43:59 -0800 (PST) 6, 23 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 23 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 23 -- by fire-doxen-ssl (Postfix) with ESMTP id 98B78328BF1; 6, 23 -- Wed, 5 Nov 2008 11:43:54 -0800 (PST) 6, 23 -- Cc: microscopy-at-microscopy.com 6, 23 -- Message-Id: {B5597E04-E6FC-452F-9FC9-E7269A513DC3-at-caltech.edu} 6, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 23 -- To: pekysar-at-ucdavis.edu 6, 23 -- In-Reply-To: {200811051334.mA5DYGxH021483-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 23 -- Subject: Re: [Microscopy] viaWWW: Processing Arabidopsis Seeds for TEM 6, 23 -- Date: Wed, 5 Nov 2008 11:43:54 -0800 6, 23 -- References: {200811051334.mA5DYGxH021483-at-ns.microscopy.com} 6, 23 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
On Nov 5, 2008, at 5:33 AM, p.lemoine-at-ulster.ac.uk wrote:
} Question: As I have no direct experience with either stm or tem,I } would apreciate comments on the following; } } 1/ Resolution is better with TEM but atomic res is still easily } obtainable with STEM, as long it is not 1 angstrom res you are } looking for (structure of carbon nanotube or graphite for instance is } easily seen with STEM) } } } 2/ STEM is much more user friendly than TEM, especially for people } who have experience in using SEM } } } 3/ STEM does not require as extensive sample thinning as TEM, TEM } needs wafer always thinner than 100nm, STEM wafers can be 1micron } (but not for ato. Res, obviously) } } } 4/ STEM allows more analysis to be done because you don't have the } lens stage below the sample, so it is easier to do EELS, electron } diffraction, EDX, ect. } } many Thanks
Dear Patrick, 1/ Since resolution depends on both the initial size of the incident beam and the extent to which it spreads as it passes through the specimen, a small beam, say 0.2 nm, and a thin specimen will allow high resolution STEM, and that resolution can be as good as TEM for a thickish specimen. 2/ I agree that STEM would likely be more user friendly for those with SEM experience, which I do not have. I do not know, however, whether there are subtleties in STEM that would be missed if one treated STEM imaging in the same way one did SEM imaging--I also have no STEM experience. 3/ Again, I'm not sure that just using thin enough STEM specimens will give good results; that depends on what you want and the nature of your specimen. For thick specimens, scattered electrons will generate signals from parts of the specimen that are outside of the area of the incident beam, which might not be relevant to your work, but which could easily have adverse effects on resolution. One may find that one needs to thin a STEM specimen to the same extent as a TEM specimen in order to get good results. 4/ Absolutely, more and different kinds of signals are usually collected in STEM than are in TEM, although a TEM/STEM machine will be capable of getting all the info available to STEM. A dedicated STEM usually is capable of better STEM performance than a TEM/STEM. It is, however, pretty easy to get EELS, ED, and EDX info from a TEM, as well as bright-field and dark-field imaging. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 23 -- From tivol-at-caltech.edu Wed Nov 5 14:19:36 2008 6, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA5KJav5002473 6, 23 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 14:19:36 -0600 6, 23 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 23 -- by fire-doxen-postvirus (Postfix) with ESMTP id 5B90C328A1A; 6, 23 -- Wed, 5 Nov 2008 12:19:36 -0800 (PST) 6, 23 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 23 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 23 -- by fire-doxen-ssl (Postfix) with ESMTP id 02D2F328BF1; 6, 23 -- Wed, 5 Nov 2008 12:19:34 -0800 (PST) 6, 23 -- Cc: microscopy-at-microscopy.com 6, 23 -- Message-Id: {F78859E8-7A9E-4FDD-9316-413F5F5B4EC9-at-caltech.edu} 6, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 23 -- To: p.lemoine-at-ulster.ac.uk 6, 23 -- In-Reply-To: {200811051333.mA5DXsiS021053-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 23 -- Subject: Re: [Microscopy] viaWWW: stem vesrus tem 6, 23 -- Date: Wed, 5 Nov 2008 12:19:34 -0800 6, 23 -- References: {200811051333.mA5DXsiS021053-at-ns.microscopy.com} 6, 23 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
First, even though you've extended processing times, try extending them some more. Especially try infiltrating in smaller increments of resin. As you've noted, the impermeable internal cell/cell wall layers are a substantial barrier to the resin, and when you have a small solvent escaping out but a large resin molecule going in, if you're not careful you get cytorrhysis or tissue collapse as well as everything else. The tannin layer is particularly painful.
The second thing we have done is to prick the seed coat to allow easier solution and resin entry - you just have to note where you've damaged the embryo.
good luck, cheers, rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334 ________________________________________ X-from: pekysar-at-ucdavis.edu [pekysar-at-ucdavis.edu] Sent: Thursday, 6 November 2008 12:42 a.m. To: White, Rosemary (PI, Black Mountain)
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Email: pekysar-at-ucdavis.edu Name: Pat Kysar
Organization: University of California, Davis
Title-Subject: [Filtered] Processing Arabidopsis Seeds for TEM
Question: Hello Listers, I have a researcher who wants to look at thin sections of Arabidopsis seeds in the TEM. He is particularly interested in the embryos and the lipid layer. The problem is that I have not been able to process them adequately. There are holes in the sections and the middle of the seeds are not fixed. I have been fixing them in 2% paraformaldehyde + 2.5% Glutaraldehyde; post fixed in reduced osmium; dehydrated with acetone and infiltrated and embedded in Epon/Araldite mixture. I use vacuum during fixation and infiltration and have extended the infiltration steps considerably (24 hours each step). From the white material I seen in some (not all) of the seeds, my guess is that the osmium is not getting past the seed coat. Does anyone have some tips I can try on these little buggers? I know there have been some threads on processing seeds but I have not been successful in locating them in the archives. Any help would be greatly appreciated.
Another thing that might work is to imbibe the seeds overnight at 4 degrees (under damp filter paper) before fixing them. This hydrated tissue then is more amenable to fixation/dehydration and embedding.
Cheers, Howard
R. Howard Berg, Ph.D. Director, Integrated Microscopy Facility Danforth Plant Science Center 975 N. Warson Rd. St. Louis, MO 63132
On Nov 5, 2008, at 3:55 PM, Rosemary.White-at-csiro.au wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Pat, } } You could try a couple of things. } } First, even though you've extended processing times, try extending } them } some more. Especially try infiltrating in smaller increments of } resin. As } you've noted, the impermeable internal cell/cell wall layers are a } substantial } barrier to the resin, and when you have a small solvent escaping out } but a } large resin molecule going in, if you're not careful you get } cytorrhysis or tissue } collapse as well as everything else. The tannin layer is } particularly painful. } } The second thing we have done is to prick the seed coat to allow } easier } solution and resin entry - you just have to note where you've } damaged the } embryo. } } good luck, } cheers, } rosemary } } Rosemary White } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } ph 61 2 6246 5475 } fx 61 2 6246 5334 } ________________________________________ } X-from: pekysar-at-ucdavis.edu [pekysar-at-ucdavis.edu] } Sent: Thursday, 6 November 2008 12:42 a.m. } To: White, Rosemary (PI, Black Mountain) } Subject: [Microscopy] viaWWW: Processing Arabidopsis Seeds for TEM } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both pekysar-at-ucdavis.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: pekysar-at-ucdavis.edu } Name: Pat Kysar } } Organization: University of California, Davis } } Title-Subject: [Filtered] Processing Arabidopsis Seeds for TEM } } Question: Hello Listers, } I have a researcher who wants to look at thin sections of Arabidopsis } seeds in the TEM. He is particularly interested in the embryos and } the lipid layer. The problem is that I have not been able to process } them adequately. There are holes in the sections and the middle of } the seeds are not fixed. } I have been fixing them in 2% paraformaldehyde + 2.5% Glutaraldehyde; } post fixed in reduced osmium; dehydrated with acetone and infiltrated } and embedded in Epon/Araldite mixture. I use vacuum during fixation } and infiltration and have extended the infiltration steps } considerably (24 hours each step). From the white material I seen in } some (not all) of the seeds, my guess is that the osmium is not } getting past the seed coat. } Does anyone have some tips I can try on these little buggers? I know } there have been some threads on processing seeds but I have not been } successful in locating them in the archives. Any help would be } greatly appreciated. } } } Login Host: 169.237.197.34 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Wed Nov 5 07:34:09 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id mA5DY9Bx021302 } 7, 11 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 } 07:34:09 -0600 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c53751410893-at-[206.69.208.22]} } 7, 11 -- Date: Wed, 5 Nov 2008 07:34:08 -0600 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: pekysar-at-ucdavis.edu (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Processing Arabidopsis Seeds for TEM } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 17, 52 -- From prvs=Rosemary.White=188f5a994-at-csiro.au Wed Nov 5 } 15:53:56 2008 } 17, 52 -- Received: from vic-MTAout3.csiro.au (vic-MTAout3.csiro.au } [150.229.64.39]) } 17, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mA5Lrsc0018988 } 17, 52 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 15:53:55 } -0600 } 17, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 17, 52 -- d=csiro.au; i=Rosemary.White-at-csiro.au; q=dns/txt; } 17, 52 -- s=email; t=1225922036; x=1257458036; } 17, 52 -- h=from:sender:reply-to:subject:date:message-id:to:cc: } 17, 52 -- mime-version:content-transfer-encoding:content-id: } 17, 52 -- content-description:resent-date:resent-from:resent- } sender: } 17, 52 -- resent-to:resent-cc:resent-message-id:in-reply-to: } 17, 52 -- references:list-id:list-help:list-unsubscribe: } 17, 52 -- list-subscribe:list-post:list-owner:list-archive; } 17, 52 -- z=From:=20 {Rosemary.White-at-csiro.au} |Subject:=20RE:=20[Micr } 17, 52 -- oscopy]=20viaWWW:=20Processing=20Arabidopsis=20Seeds=20fo } 17, 52 -- r=20TEM|Date:=20Thu,=206=20Nov=202008=2008:53:53=20+1100 } 17, 52 -- |Message-ID:=20 {0A5E41F72813C247889F7974C8D764955BF22F6A6 } 17, 52 -- F-at-exvic-mbx04.nexus.csiro.au} |To:=20 {pekysar-at-ucdavis.edu} } 17, 52 -- ,=20 {microscopy-at-microscopy.com} |MIME-Version:=201.0 } 17, 52 -- |Content-Transfer-Encoding:=20quoted-printable } 17, 52 -- |In-Reply-To:=20 {200811051342.mA5Dgmbq016276-at-ns.microscop } 17, 52 -- y.com} |References:=20 {200811051342.mA5Dgmbq016276-at-ns.micr } 17, 52 -- oscopy.com} ; } 17, 52 -- bh=zw3bFjBbI4Wu7Kr/ZzFt1fE+7MgpP/fjzJ3hV3EAcOg=; } 17, 52 -- b=ciTtfA8ANRHAiHw6V3v7nPpyKgeIsr30dnS5fsMzROnGv4eH3Da5uOh3 } 17, 52 -- qexwdzd3+F1RxTDXzK7VDQkQNiH+XOTaxycuJBjCq2a34pSZqkdAce9UY } 17, 52 -- a7H7Tmww83nUMW1; } 17, 52 -- X-IronPort-AV: E=Sophos;i="4.33,552,1220191200"; } 17, 52 -- d="scan'208";a="2712074" } 17, 52 -- Received: from exvic-htca01.nexus.csiro.au } ([138.194.81.126]) } 17, 52 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 06 } Nov 2008 08:53:53 +1100 } 17, 52 -- Received: from exvic-mbx04.nexus.csiro.au } ([138.194.81.124]) by } 17, 52 -- exvic-htca01.nexus.csiro.au ([138.194.81.126]) with mapi; } Thu, 6 Nov 2008 } 17, 52 -- 08:53:53 +1100 } 17, 52 -- From: {Rosemary.White-at-csiro.au} } 17, 52 -- To: {pekysar-at-ucdavis.edu} , {microscopy-at-microscopy.com} } 17, 52 -- Date: Thu, 6 Nov 2008 08:53:53 +1100 } 17, 52 -- Subject: RE: [Microscopy] viaWWW: Processing Arabidopsis } Seeds for TEM } 17, 52 -- Thread-Topic: [Microscopy] viaWWW: Processing Arabidopsis } Seeds for TEM } 17, 52 -- Thread-Index: Ack/TGn9v/G9jXKtQyasL/wqVU/PZQAQzcdm } 17, 52 -- Message-ID: {0A5E41F72813C247889F7974C8D764955BF22F6A6F-at-exvic-mbx04.nexus.csiro.au } } } 17, 52 -- References: {200811051342.mA5Dgmbq016276-at-ns.microscopy.com} } 17, 52 -- In-Reply-To: {200811051342.mA5Dgmbq016276-at-ns.microscopy.com} } 17, 52 -- Accept-Language: en-US, en-AU } 17, 52 -- Content-Language: en-NZ } 17, 52 -- X-MS-Has-Attach: } 17, 52 -- X-MS-TNEF-Correlator: } 17, 52 -- acceptlanguage: en-US, en-AU } 17, 52 -- Content-Type: text/plain; charset="us-ascii" } 17, 52 -- MIME-Version: 1.0 } 17, 52 -- Content-Transfer-Encoding: 8bit } 17, 52 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mA5Lrsc0018988 } ==============================End of - } Headers==============================
==============================Original Headers============================== 13, 23 -- From RHBerg-at-danforthcenter.org Wed Nov 5 17:40:24 2008 13, 23 -- Received: from spm1.ddpsc.org (spm1.danforthcenter.org [65.254.111.26]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA5NeOqg003134 13, 23 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 5 Nov 2008 17:40:24 -0600 13, 23 -- Received: from spm1.ddpsc.org (127.0.0.1) by spm1.ddpsc.org (MlfMTA v3.2r9) id h28neg0171s9 for {microscopy-at-sparc5.microscopy.com} ; Wed, 5 Nov 2008 17:40:23 -0600 (envelope-from {RHBerg-at-danforthcenter.org} ) 13, 23 -- Received: from mail2.ddpsc.org ([10.101.0.44]) 13, 23 -- by spm1.ddpsc.org (SonicWALL 6.2.3.1217) 13, 23 -- with ESMTP; Wed, 05 Nov 2008 17:40:23 -0600 13, 23 -- Received: from [10.14.0.126] (10.14.0.126) by mail2.ddpsc.org (10.101.0.44) 13, 23 -- with Microsoft SMTP Server id 8.1.311.2; Wed, 5 Nov 2008 17:40:23 -0600 13, 23 -- Message-ID: {8BFE7402-A3E9-44D6-9DCE-861CBAEF2F11-at-danforthcenter.org} 13, 23 -- From: Howard Berg {RHBerg-at-danforthcenter.org} 13, 23 -- To: {microscopy-at-ns.microscopy.com} 13, 23 -- In-Reply-To: {200811052155.mA5LtTw7021224-at-ns.microscopy.com} 13, 23 -- Content-Type: text/plain; charset="US-ASCII"; format=flowed; delsp=yes 13, 23 -- Content-Transfer-Encoding: 7bit 13, 23 -- MIME-Version: 1.0 (Apple Message framework v929.2) 13, 23 -- Subject: Re: [Junk released by Policy action] [Microscopy] RE: viaWWW: Processing Arabidopsis Seeds for TEM 13, 23 -- Date: Wed, 5 Nov 2008 17:40:23 -0600 13, 23 -- References: {200811052155.mA5LtTw7021224-at-ns.microscopy.com} 13, 23 -- X-Mailer: Apple Mail (2.929.2) 13, 23 -- X-Mlf-Version: 6.2.3.1217 13, 23 -- X-Mlf-UniqueId: o200811052340230087819 ==============================End of - Headers==============================
I have learned that when I tried to section hard things I could get good sections if I bottom trimmed with a glass knife INTO the specimen about 0.5 micron at a time THEN faced the block the same way. When the sectioning begins, the specimen hits the knife edge at the bottom of the section. I got good sections whereas I got nothing useable if I had the material completely surrounded with embedding material. The surrounding embedding material is capable of holding the specimen in place for sectioning. The specimen may still separate from the epon or Spurr's in the knife boat but that usually does not matter.
Sorry but I do not remember the particular protocols that were mentioned for fixation and dehydration of seeds.
Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {tivol-at-caltech.edu} } Reply-To: {tivol-at-caltech.edu} } Date: Wed, 5 Nov 2008 13:48:12 -0600 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] Re: viaWWW: Processing Arabidopsis Seeds for TEM } } On Nov 5, 2008, at 5:34 AM, pekysar-at-ucdavis.edu wrote: } } } Does anyone have some tips I can try on these little buggers? I know } } there have been some threads on processing seeds but I have not been } } successful in locating them in the archives. Any help would be } } greatly appreciated. } } } Dear Pat, } I have had only very limited experience examining seeds, but } preparation is definitely the key. The person who embedded and } sectioned the seeds also found that the knife would often pull the } seeds out of the resin, leaving holes. We did have some success by } altering the composition of the resin until there was a good match of } the hardness of the resin with that of the seeds (germinating pine, in } this case). You will have to experiment with compositions until you } find what works for your specimen, and, since I was doing } microanalysis, I didn't want to stain the seeds, so the composition } that worked for us is not necessarily going to work for you (even if I } could remember what it was). Good luck. } Yours, } Bill Tivol, PhD } EM Scientist } Ultrafast EM Facility } Noyes Laboratory, MC 127-72 } California Institute of Technology } Pasadena CA 91125 } (626) 395-8833 } tivol-at-caltech.edu
} ==============================End of - Headers==============================
==============================Original Headers============================== 10, 26 -- From connellyps-at-nhlbi.nih.gov Wed Nov 5 19:24:13 2008 10, 26 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA61OD7O018979 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 5 Nov 2008 19:24:13 -0600 10, 26 -- X-IronPortListener: Outbound_SMTP 10, 26 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 10, 26 -- by nihxway2out.hub.nih.gov with ESMTP; 05 Nov 2008 20:24:13 -0500 10, 26 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 10, 26 -- Wed, 5 Nov 2008 20:24:09 -0500 10, 26 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.161]) with Microsoft Exchange Server HTTP-DAV ; 10, 26 -- Thu, 6 Nov 2008 01:24:08 +0000 10, 26 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 26 -- Date: Wed, 05 Nov 2008 20:23:50 -0500 10, 26 -- Subject: Re: [Microscopy] Re: viaWWW: Processing Arabidopsis Seeds for TEM 10, 26 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 26 -- Message-ID: {C537B156.2893%connellyps-at-nhlbi.nih.gov} 10, 26 -- Thread-Topic: [Microscopy] Re: viaWWW: Processing Arabidopsis Seeds for TEM 10, 26 -- Thread-Index: Ack/rlKikWzInquhEd2UlwANk2Yv1A== 10, 26 -- In-Reply-To: {200811051948.mA5JmCgk028034-at-ns.microscopy.com} 10, 26 -- Mime-version: 1.0 10, 26 -- Content-type: text/plain; 10, 26 -- charset="ISO-8859-1" 10, 26 -- X-OriginalArrivalTime: 06 Nov 2008 01:24:09.0371 (UTC) FILETIME=[5E2E1EB0:01C93FAE] 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA61OD7O018979 ==============================End of - Headers==============================
There should be a manometer (or is it called a pressure gauge?) continuously displaying the pression in the tank. I consider it enough to indicate a leak and take action if necessary. Just check the pressure each time you enter the room.
A far as I know, a TEM room should be air conditionned at least to avoid temperature fluctuations. Just make sure that the air is pumped from the floor level and not from the ceiling and the risks should approach zero. I must also say that the volume of this chamber is pretty limited too.
Best regards, Stephane
----- Original Message ---- X-from: "mike.flaws-at-canterbury.ac.nz" {mike.flaws-at-canterbury.ac.nz} To: nizets2-at-yahoo.com Sent: Wednesday, November 5, 2008 2:40:12 PM
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Email: mike.flaws-at-canterbury.ac.nz Name: Mike Flaws
Organization: University of Canterbury
Title-Subject: [Filtered] SF6 Detector necessary?
Question: Hi, Ý I am about to install a TEM that has SF6 in the HT tank. I have read that an SF6 detector should be used to continuously monitor the air in the room for leaks. Is this commonly done or is the likelihood of a leak so remote that it is unnecessary? Ý There is a big difference in the price of a detector that monitors continuously vs one that is used for occasional use - such as when the filament is replaced. Ý Thanks in advance for your help. Ý Mike
The Midwest Microscopy and Microanalysis Society will hold its final meeting of the year, a Symposium on Microscopy, on Friday, 21 November, 2008. The workshop will take place at Baxter Corporate Headquarters in Deerfield, IL. Program and registration information can be found on our website:
www.midwestmicroscopy.org
Please follow the link to the Meetings page. Inquiries and RSVPs can be directed to mierzwa-at-jeol.com, or call 920-803-8945.
Regards,
Elaine
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==============================Original Headers============================== 10, 23 -- From eschumacher-at-mccrone.com Thu Nov 6 08:36:02 2008 10, 23 -- Received: from oma.mccrone.com (mail.mccrone.com [12.54.22.114]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA6Ea1TR017379 10, 23 -- for {microscopy-at-microscopy.com} ; Thu, 6 Nov 2008 08:36:01 -0600 10, 23 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) by oma.mccrone.com with Microsoft SMTPSVC(6.0.3790.3959); 10, 23 -- Thu, 6 Nov 2008 08:36:01 -0600 10, 23 -- Content-class: urn:content-classes:message 10, 23 -- MIME-Version: 1.0 10, 23 -- Content-Type: text/plain; 10, 23 -- charset="iso-8859-1" 10, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 23 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society 10, 23 -- Date: Thu, 6 Nov 2008 08:35:25 -0600 10, 23 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150E1D0-at-MCCRONEMSG.tmg.mccrone.com} 10, 23 -- X-MS-Has-Attach: 10, 23 -- X-MS-TNEF-Correlator: 10, 23 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society 10, 23 -- Thread-Index: AclAHOfDQvi70jehQNeKHkCFDGOlMg== 10, 23 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 10, 23 -- To: {microscopy-at-microscopy.com} 10, 23 -- X-OriginalArrivalTime: 06 Nov 2008 14:36:01.0820 (UTC) FILETIME=[FDCE7DC0:01C9401C] 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA6Ea1TR017379 ==============================End of - Headers==============================
Image Core Facility, at Skirball Institute of New York University School of Medicine is seeking a full-time staff member. The Image core facility provides state-of-the-art imaging and visualization resources for the School of Medicine and local scientific community.
We seek a dynamic, motivated individual with expertise in current techniques for fluorescence imaging of cells and tissues. Candidates must have demonstrated expertise in confocal microscopy in fixed and live cells (tissues). Experience with software for image analysis and data presentation is essential. Minimal requirements include a bachelor degree in scientific field, and 2-5years experience in advanced fluorescence microscopy imaging techniques. Masters degree in biology is preferred, and specific experience in electron microscopy is highly desirable. The successful candidate will coordinate the operation of the light microscopy resources of the image core facility, train users in microscope operation and advanced imaging techniques, and assist sample preparation of electron microscopy.
To apply, a curriculum vita, a cover letter including statement of interests, salary requirements and contact information for three references should be e-mailed to: Fengxia.Liang-at-med.nyu.edu.
Alice
-- Alice F. Liang, Ph.D. Director, Image Core Facility Skirball Institute of Biomolecular Medicine Skirball 2nd floor, EM Suite New York University School of Medicine New York, NY 10016 Tel: 212-263-7644 (o); 212-263-7099 (Lab) Fax: 212-263-7643 E-mail: Fengxia.Liang-at-med.nyu.edu http://saturn.med.nyu.edu/facilities/imagecore/
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==============================Original Headers============================== 8, 24 -- From Fengxia.Liang-at-med.nyu.edu Thu Nov 6 09:07:50 2008 8, 24 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA6F7nbf031890 8, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Nov 2008 09:07:49 -0600 8, 24 -- X-IronPort-AV: E=Sophos;i="4.33,557,1220241600"; 8, 24 -- d="scan'208";a="40166920" 8, 24 -- Received: from msgwsdcpbh61.nyumc.org ([10.147.36.107]) 8, 24 -- by mail4.nyumc.org with ESMTP; 06 Nov 2008 10:07:46 -0500 8, 24 -- Received: from MSGWSDCPMB02.nyumc.org ([10.147.37.225]) by msgwsdcpbh61.nyumc.org with Microsoft SMTPSVC(6.0.3790.3959); 8, 24 -- Thu, 6 Nov 2008 10:07:45 -0500 8, 24 -- Received: from 128.122.135.9 ([128.122.135.9]) by MSGWSDCPMB02.nyumc.org ([10.147.37.24]) via Exchange Front-End Server mail.nyumc.org ([10.134.254.78]) with Microsoft Exchange Server HTTP-DAV ; 8, 24 -- Thu, 6 Nov 2008 15:07:45 +0000 8, 24 -- User-Agent: Microsoft-Entourage/12.11.0.080522 8, 24 -- Date: Thu, 06 Nov 2008 10:03:20 -0500 8, 24 -- Subject: Position opening 8, 24 -- From: Alice Liang {Fengxia.Liang-at-med.nyu.edu} 8, 24 -- To: {Microscopy-at-microscopy.com} 8, 24 -- Message-ID: {C5387168.2EA61%Fengxia.Liang-at-med.nyu.edu} 8, 24 -- Thread-Topic: Position opening 8, 24 -- Thread-Index: AclAIM48g5IKsJpaRYGD32gpQ92sVQ== 8, 24 -- Mime-version: 1.0 8, 24 -- X-OriginalArrivalTime: 06 Nov 2008 15:07:45.0881 (UTC) FILETIME=[6CB72490:01C94021] 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] part for Technics sputter coater
Question: we are in need of a replacement glass chamber for a Technics Hummer-II sputter coater. It is approx 25-30 years old. I have not been able to locate a source for parts--not sure if parts are even still available for the old Hummers. Perhaps it would be a better approach to explore having a replacement chamber fabricated? Any advice or suggestions are appreciated.
International Conference on PROCESSING & MANUFACTURING OF ADVANCED MATERIALS Processing, Fabrication, Properties, Applications August 25-29, 2009, Technical University-Berlin, Germany
http://thermec.uow.edu.au/index.html
best regards
Chris Hübner
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==============================Original Headers============================== 9, 28 -- From hubner-at-iod.krakow.pl Fri Nov 7 01:04:19 2008 9, 28 -- Received: from sowa.iod.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA774IpO006643 9, 28 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 01:04:19 -0600 9, 28 -- Received: from SERWER_SQL (serwer_sql [149.156.29.202]) 9, 28 -- by sowa.iod.krakow.pl (8.13.6+Sun/8.13.6) with SMTP id mA76i8HH020342; 9, 28 -- Fri, 7 Nov 2008 07:44:08 +0100 (CET) 9, 28 -- Received: from [149.156.29.4] (helo=iodkh007) 9, 28 -- by SERWER_SQL with AVK MailGateway; 9, 28 -- Fri, 07 Nov 2008 08:04:04 +0100 9, 28 -- Date: Fri, 7 Nov 2008 08:04:16 +0100 9, 28 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-iod.krakow.pl} 9, 28 -- To: "Materials" {MATERIALS-at-liverpool.ac.uk} , 9, 28 -- "Microscopy list" {microscopy-at-microscopy.com} 9, 28 -- MIME-Version: 1.0 9, 28 -- Message-ID: {A290DD891D30483DB43B9E0F28EB470B-at-iodkh007} 9, 28 -- Subject: THERMEC' 2009 9, 28 -- Content-Type: text/plain; 9, 28 -- charset="iso-8859-2"; 9, 28 -- format="flowed"; 9, 28 -- reply-type="original" 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-Priority: 3 9, 28 -- X-MSMail-Priority: Normal 9, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 9, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 9, 28 -- X-AVK-Virus-Check: AVF 19.135;F1206 9, 28 -- X-AVK-Virus-Check: AVF 19.135;06.11.2008 ==============================End of - Headers==============================
DISCLAIMER: Ladd Research sells microscopy supplies including Hummer Sputter coaters.
At 07:54 PM 11/6/2008, you wrote:
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==============================Original Headers============================== 15, 27 -- From jd-at-laddresearch.com Fri Nov 7 07:39:29 2008 15, 27 -- Received: from worden.electric.net (worden.electric.net [72.35.23.25]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA7DdSsx003187 15, 27 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 07:39:29 -0600 15, 27 -- Received: from 1KyRYd-0002F1-W3 by worden.electric.net with emc1-ok (Exim 4.69) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1KyRYe-0002FM-Tj; Fri, 07 Nov 2008 05:39:28 -0800 15, 27 -- Received: by emcmailer; Fri, 07 Nov 2008 05:39:28 -0800 15, 27 -- Received: from [216.204.198.170] (helo=NewServer.laddresearch.com) 15, 27 -- by worden.electric.net with esmtps (TLSv1:AES256-SHA:256) 15, 27 -- (Exim 4.69) 15, 27 -- (envelope-from {jd-at-laddresearch.com} ) 15, 27 -- id 1KyRYd-0002F1-W3; Fri, 07 Nov 2008 05:39:28 -0800 15, 27 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 15, 27 -- Date: Fri, 07 Nov 2008 08:39:24 -0500 15, 27 -- To: dlowry-at-asu.edu 15, 27 -- From: jd {jd-at-laddresearch.com} 15, 27 -- Subject: Re: [Microscopy] viaWWW: part for Technics sputter coater 15, 27 -- Cc: Microscopy listserver {microscopy-at-microscopy.com} 15, 27 -- In-Reply-To: {200811070054.mA70sQhp024204-at-ns.microscopy.com} 15, 27 -- References: {200811070054.mA70sQhp024204-at-ns.microscopy.com} 15, 27 -- Mime-Version: 1.0 15, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 15, 27 -- X-Outbound-IP: 216.204.198.170 15, 27 -- X-Env-From: jd-at-laddresearch.com 15, 27 -- X-Virus-Status: Scanned by VirusSMART (c) 15, 27 -- Message-Id: {E1KyRYe-0002FM-Tj-at-worden.electric.net} ==============================End of - Headers==============================
Does ASU have a glass shop in the Chemistry department (or Chem. E.)? If yes, you can likely get a chamber from them. It's just a section of glass tubing, really. Give them the dimensions (including wall thickness), and they'll be able to cut a piece to size and fire-polish the ends for a good vacuum seal. Just stress that the ends have to be perfectly square. I had the same problem in the past (at UWisc.), and this fix worked well, and was significantly cheaper than a commercial part. The chamber can also be made from Lexan or Plexiglas tubing The caveat is that tubing in this diameter can be hard to find, so when you do find it, get 2 (or 3, if glass) made. Stick the spare somewhere safe.
Phil
} Email: dlowry-at-asu.edu } Name: David Lowry } } Organization: Arizona State University } } Title-Subject: [Filtered] part for Technics sputter coater } } Question: we are in need of a replacement glass chamber for a } Technics Hummer-II sputter coater. It is approx 25-30 years old. I } have not been able to locate a source for parts--not sure if parts } are even still available for the old Hummers. Perhaps it would be a } better approach to explore having a replacement chamber fabricated? } Any advice or suggestions are appreciated. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 26 -- From oshel1pe-at-cmich.edu Fri Nov 7 08:00:54 2008 4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA7E0rwK019064 4, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 08:00:54 -0600 4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mA7E0FOu009287; 4, 26 -- Fri, 7 Nov 2008 09:00:42 -0500 4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 26 -- Fri, 7 Nov 2008 09:00:39 -0500 4, 26 -- Mime-Version: 1.0 4, 26 -- Message-Id: {f06240801c539f96483f7-at-[141.209.160.249]} 4, 26 -- In-Reply-To: {200811070052.mA70qVTv021343-at-ns.microscopy.com} 4, 26 -- References: {200811070052.mA70qVTv021343-at-ns.microscopy.com} 4, 26 -- Date: Fri, 7 Nov 2008 09:00:38 -0500 4, 26 -- To: dlowry-at-asu.edu 4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 26 -- Subject: Re: [Microscopy] viaWWW: part for Technics sputter coater 4, 26 -- Cc: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 26 -- X-OriginalArrivalTime: 07 Nov 2008 14:00:39.0896 (UTC) FILETIME=[37745580:01C940E1] 4, 26 -- X-Canit-CHI2: 0.00 4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 26 -- X-CanItPRO-Stream: default 4, 26 -- X-Canit-Stats-ID: 5000035 - 485bfdbce698 4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
On Nov 6, 2008, at 1:49 AM, nizets2-at-yahoo.com wrote:
} A far as I know, a TEM room should be air conditionned at least to } avoid temperature fluctuations. Just make sure that the air is } pumped from the floor level and not from the ceiling and the risks } should approach zero. I must also say that the volume of this } chamber is pretty limited too.
Dear Stephane, When the room for our scopes were "designed" by the building architects, they decided that the floor-level SF6 exhaust ports would make swell A/C return ducts as well. There were only two serious flaws with this. First, the A/C inlet dropped cold air through a special laminar flow duct, and the air continued to the floor, under the curtains, then out the SF6 duct, never coming close to the column, which sat in warm dead air. Second, the quantity of the exhaust caused a high level of acoustic vibrations in the room. The Provost was very generous, spending } $100k to retrofit the room, which the engineers determined needed 60 ft^2 (~6 m^2) of return duct area to meet both the temperature stability and air flow velocity specs. The SF6 tanks for our TF30 and T20 are ~2 m tall by ~0.7 m diameter at a pressure of a few bar, which is the equivalent of ~3 m^3. The smaller room with SF6 is ~36 m^3, so the total SF6 volume is a significant fraction, but, as was pointed out, SF6 is an asphyxiant, not a toxin, so it would take a very high concentration to lower the oxygen content to a dangerous level. As long as there is enough air flow to mix the SF6, there should be no danger, and if any did exist, there would be no problem unless one was constrained to breath near the floor, so it would be dangerous either to be working near the floor or to have lost consciousness when the SF6 tank emptied. That being said, however, I am strongly in favor of making the workplace as safe as is humanly possible, so I am glad we have the SF6 exhaust ports near the floor when needed. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Nov 7 13:47:16 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA7JlG1A019947 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 13:47:16 -0600 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id A924566E0A3E 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 11:47:15 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 9FD8A66E0811 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 11:47:14 -0800 (PST) 6, 22 -- Message-Id: {9E118AF6-9F0E-46E5-92A7-2C02AED50A05-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200811060949.mA69nlc6022855-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] Re: viaWWW: SF6 Detector necessary 6, 22 -- Date: Fri, 7 Nov 2008 11:47:13 -0800 6, 22 -- References: {200811060949.mA69nlc6022855-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
We also recently went thru a TEM suite remodel in which we placed a FEI Tecnai G2 TEM which uses SF6 gas in the emission chamber and also within the HV tank. The installation guide clearly states the need for an SF6 detector in the room, which caused us a great deal of worry and potential expenditure (an SF6 detector can be very expensive). We also had issue with Nitrogen in the space, as we work with lots of LN2 in the room while using the cryostage. SF6 is not toxic in itself, but like nitrogen can displace oxygen in the room from the floor upwards, as suggested by others. So really what is most important is to monitor Oxygen levels, also to exhaust from the floor when low levels of O2 are detected. Our solution was to mount a comparatively inexpensive Oxygen sensor at about 16 inches from the floor and hook up an exhaust fan which is tripped when low oxygen levels in the room are detected. This exhaust goes directly outside.
Best,
Doug Keene Micro-Imaging Center Shriners Hospital, Portland, Or.
-----Original Message----- X-from: tivol-at-caltech.edu [mailto:tivol-at-caltech.edu] Sent: Friday, November 07, 2008 11:53 AM To: Doug Keene
On Nov 6, 2008, at 1:49 AM, nizets2-at-yahoo.com wrote:
} A far as I know, a TEM room should be air conditionned at least to } avoid temperature fluctuations. Just make sure that the air is } pumped from the floor level and not from the ceiling and the risks } should approach zero. I must also say that the volume of this } chamber is pretty limited too.
Dear Stephane, When the room for our scopes were "designed" by the building architects, they decided that the floor-level SF6 exhaust ports would make swell A/C return ducts as well. There were only two serious flaws with this. First, the A/C inlet dropped cold air through a special laminar flow duct, and the air continued to the floor, under the curtains, then out the SF6 duct, never coming close to the column, which sat in warm dead air. Second, the quantity of the exhaust caused a high level of acoustic vibrations in the room. The Provost was very generous, spending } $100k to retrofit the room, which the engineers determined needed 60 ft^2 (~6 m^2) of return duct area to meet both the temperature stability and air flow velocity specs. The SF6 tanks for our TF30 and T20 are ~2 m tall by ~0.7 m diameter at a pressure of a few bar, which is the equivalent of ~3 m^3. The smaller room with SF6 is ~36 m^3, so the total SF6 volume is a significant fraction, but, as was pointed out, SF6 is an asphyxiant, not a toxin, so it would take a very high concentration to lower the oxygen content to a dangerous level. As long as there is enough air flow to mix the SF6, there should be no danger, and if any did exist, there would be no problem unless one was constrained to breath near the floor, so it would be dangerous either to be working near the floor or to have lost consciousness when the SF6 tank emptied. That being said, however, I am strongly in favor of making the workplace as safe as is humanly possible, so I am glad we have the SF6 exhaust ports near the floor when needed. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Nov 7 13:47:16 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA7JlG1A019947 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 13:47:16 -0600 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id A924566E0A3E 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 11:47:15 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 9FD8A66E0811 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 11:47:14 -0800 (PST) 6, 22 -- Message-Id: {9E118AF6-9F0E-46E5-92A7-2C02AED50A05-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200811060949.mA69nlc6022855-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] Re: viaWWW: SF6 Detector necessary 6, 22 -- Date: Fri, 7 Nov 2008 11:47:13 -0800 6, 22 -- References: {200811060949.mA69nlc6022855-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 27 -- From drk-at-shcc.org Fri Nov 7 15:36:32 2008 16, 27 -- Received: from mail.shcc.org (shcc.org [64.213.211.200]) 16, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA7LaWIk004656 16, 27 -- for {Microscopy-at-Microscopy.Com} ; Fri, 7 Nov 2008 15:36:32 -0600 16, 27 -- Received: from por-ex-ch01.research.shcc.org (64.213.211.212) by mail.shcc.org 16, 27 -- (64.213.211.200) with Microsoft SMTP Server (TLS) id 8.0.813.0; Fri, 7 Nov 16, 27 -- 2008 13:36:29 -0800 16, 27 -- Received: from por-ex-mb01.research.shcc.org ([64.213.211.102]) by 16, 27 -- por-ex-ch01.research.shcc.org ([64.213.211.212]) with mapi; Fri, 7 Nov 2008 16, 27 -- 13:41:45 -0800 16, 27 -- From: Doug Keene {drk-at-shcc.org} 16, 27 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 16, 27 -- Date: Fri, 7 Nov 2008 13:42:40 -0800 16, 27 -- Subject: RE: [Microscopy] viaWWW: SF6 Detector necessary 16, 27 -- Thread-Topic: [Microscopy] viaWWW: SF6 Detector necessary 16, 27 -- Thread-Index: AclBEsblsfZTN9WXT7Kb92K82C6TuwADYzwg 16, 27 -- Message-ID: {145D57516E39804A832D96C2EC1C553F174BB513CE-at-por-ex-mb01.research.shcc.org} 16, 27 -- In-Reply-To: {200811071952.mA7JqnF1026353-at-ns.microscopy.com} 16, 27 -- Accept-Language: en-US 16, 27 -- Content-Language: en-US 16, 27 -- X-MS-Has-Attach: 16, 27 -- X-MS-TNEF-Correlator: 16, 27 -- acceptlanguage: en-US 16, 27 -- Content-Type: text/plain; charset="us-ascii" 16, 27 -- MIME-Version: 1.0 16, 27 -- Content-Transfer-Encoding: 8bit 16, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mA7LaWIk004656 ==============================End of - Headers==============================
On Nov 7, 2008, at 1:36 PM, drk-at-shcc.org wrote:
} Our solution was to mount a comparatively inexpensive Oxygen sensor } at about 16 inches from the floor and hook up an exhaust fan which } is tripped when low oxygen levels in the room are detected.
Dear Doug, Good solution, but I'd suggest moving the sensor closer to the floor, since the truly dangerous situation is if someone--working on the HT tank, say--has an accident resulting in loss of consciousness. In that case, the person's head will be lower than 16", and there could be sufficient SF6 concentration to cause asphyxiation. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Nov 7 16:15:04 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA7MF4XA019419 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 16:15:04 -0600 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 6E06C66E0816 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 14:15:03 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id 561B066E016B 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 14:15:02 -0800 (PST) 6, 22 -- Message-Id: {C8A653D8-5090-4EAA-909F-F1745E6335ED-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200811072136.mA7LadxU004785-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] RE: viaWWW: SF6 Detector necessary 6, 22 -- Date: Fri, 7 Nov 2008 14:15:01 -0800 6, 22 -- References: {200811072136.mA7LadxU004785-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
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Email: gw265-at-cam.ac.uk Name: Giselle Walker
Organization: University of Cambridge
Title-Subject: [Filtered] How much is a Reichert Ultracut S worth?
Question: Does anyone know roughly how much a Reichert Ultracut S, ca. 1993 vintage, is worth at the moment?
I'm interested in putting in a bid for a bit of university -owned equipment which has sat unused for the last 8 years. It's a lovely Reichert Ultracut S ultramicrotome, in excellent working order as of 8 years ago. However, in the interim, the ultramicrotome has been moved twice (not necessarily particularly carefully), and the cable joining the control pad to the ultramicrotome has been lost.
I am planning to bid whatever an ultracut S is worth these days, minus the cost of a new cable ($650 AUD!) and minus the cost of a thorough service (if anyone knows of any good service people in Sydney, Australia or in the southeast of the UK, i'd be delighted to hear the details).
In my humble opinion, the Ultracut S is likely to be a problem for owners in the future. While it IS a nice microtome if working, it's electronic technology is that awkward stage of advancement in which the fine new features may be difficult to support in the not so distant future. The A-O Ultracut and Reichert Ultracut-E are models that I am familiar with and these are mechanically robust and the electronics are simple enough that they can run another few decades or more. However, these models have been dropped from official support so owners are dependent on the excellent aftermarket care that some vendors provide. The "S" is still on the Leica support list last I knew, but this model has a "main board" with 3 microcontrollers to manage the optical encoder, display, and other functions and these boards are not stocked but made as needed at a price of } $2000 USD, and the optical encoder board was another $1000+; my experience was that they replace the the full boards, 30da delivery (since they are made to order in Austria, probably from diminishing stocks of parts...). I suspect that supporting this vintage of electronics is getting to be an albatross for Leica and wouldn't be surprised to see if fall off the "supported" list soon.
So my advice is to inquire about service, prices, and time of commitment to future of support for this model (from Leica) before you buy. If you are interested in using and maintaining this model, proceed with caution. One of the older models may be a better way to go for an individual looking for economical long-term use.
In answer to your question, I wouldn't pay much for it for the reasons above, so I hope you can get it cheaply, in spite of the once-high cost, if you do choose to buy it.
I have a dead Ultracut-S, 2 Ultracut-E's and an A-O Ultracut.
Dale Callaham University of Massachusetts -at- Amherst
gw265-at-cam.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: gw265-at-cam.ac.uk } Name: Giselle Walker } } Organization: University of Cambridge } } Title-Subject: [Filtered] How much is a Reichert Ultracut S worth? } } Question: Does anyone know roughly how much a Reichert Ultracut S, } ca. 1993 vintage, is worth at the moment? } } I'm interested in putting in a bid for a bit of university -owned } equipment which has sat unused for the last 8 years. It's a lovely } Reichert Ultracut S ultramicrotome, in excellent working order as of } 8 years ago. However, in the interim, the ultramicrotome has been } moved twice (not necessarily particularly carefully), and the cable } joining the control pad to the ultramicrotome has been lost. } } I am planning to bid whatever an ultracut S is worth these days, } minus the cost of a new cable ($650 AUD!) and minus the cost of a } thorough service (if anyone knows of any good service people in } Sydney, Australia or in the southeast of the UK, i'd be delighted to } hear the details). } } Thanks, } } Giselle } } Dr. Giselle Walker } Electron-microscopist-at-large } } } Login Host: 137.82.136.197 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 12, 11 -- From zaluzec-at-microscopy.com Fri Nov 7 18:14:15 2008 } 12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA80EDn9007943 } 12, 11 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 18:14:14 -0600 } 12, 11 -- Mime-Version: 1.0 } 12, 11 -- Message-Id: {p06240800c53a8a4368b6-at-[206.69.208.22]} } 12, 11 -- Date: Fri, 7 Nov 2008 18:14:12 -0600 } 12, 11 -- To: microscopy-at-microscopy.com } 12, 11 -- From: gw265-at-cam.ac.uk (by way of MicroscopyListserver) } 12, 11 -- Subject: viaWWW: How much is a Reichert Ultracut S worth? } 12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 20 -- From dac-at-research.umass.edu Fri Nov 7 18:59:53 2008 8, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mA80xqKk022015 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 7 Nov 2008 18:59:53 -0600 8, 20 -- Received: from [192.168.1.101] ([96.39.6.64]) 8, 20 -- (authenticated bits=0) 8, 20 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id mA80xmgD001374 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 20 -- Fri, 7 Nov 2008 19:59:49 -0500 8, 20 -- Message-ID: {4914E48C.10004-at-research.umass.edu} 8, 20 -- Date: Fri, 07 Nov 2008 19:59:56 -0500 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.17) Gecko/20080829 SeaMonkey/1.1.12 8, 20 -- MIME-Version: 1.0 8, 20 -- To: gw265-at-cam.ac.uk, Microscopy List {microscopy-at-microscopy.com} 8, 20 -- Subject: Re: [Microscopy] viaWWW: How much is a Reichert Ultracut S worth? 8, 20 -- References: {200811080019.mA80JfQg016120-at-ns.microscopy.com} 8, 20 -- In-Reply-To: {200811080019.mA80JfQg016120-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear friends, this e-mail is to announce the Intensive Practical School on Confocal and Multiphoton MIcroscopy at LAMBS - www.lambs.it under the auspices of the Italian Society of Microscopical Sciences (www.sism.it). You can find the brochure at www.lambs.it, www.sism.it or request it via e- mail. Lecturers will be: Bizzarri Ranieri (NEST-INFM, SNS, Pisa, I) Borlinghaus Rolf (Leica Microsystems, D) Bunt Gertrude (University Gottingen,D) Collini Maddalena (Università Milano-Bicocca,I) Diaspro Alberto (Università di Genova, I) Faretta Mario (IFOM-IEO, Milano, I) Ratto Gimmi (CNR, Pisa, I) Usai Cesare (CNR, Genova, I) Wilson Tony (University of Oxford,Oxford, U.K.) Wouters Fred (University Gottingen, D)
Practical sessions will be realized by Lecturers and LAMBS members using the newest Conofcal set-up, Multiphoton Workstations and Timelapse workstations.
SInce there is a limited number of students that will be acceted please book ASAP! All the best Alby
----------------------------------------------------- Resistere, Resistere, Resistere! Hold out, Hold out, Hold out! ----------------------------------------------------- Alberto Diaspro Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy fax +39-010314218 - tel +39 0103536426/309; email: diaspro-at-fisica.unige.it - URL: www.lambs.it;
Dear Microscopists, We are in need of service for our MT-5000 ultramicrotome and would appreciate any leads for qualified service engineers in the Boston area. Many Thanks. Vachik Hacopian
==============================Original Headers============================== 3, 18 -- From vhacopia-at-firstclass.wellesley.edu Mon Nov 10 10:56:15 2008 3, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAAGuDqZ003784 3, 18 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2008 10:56:14 -0600 3, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) 3, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id mAAGu8Rh029824 3, 18 -- for {microscopy-at-microscopy.com} ; Mon, 10 Nov 2008 11:56:08 -0500 (EST) 3, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) 3, 18 -- Message-id: {fc.006640d81ae251d1006640d81ae251d1.1ae8823f-at-firstclass.wellesley.edu} 3, 18 -- Date: Mon, 10 Nov 2008 11:51:31 -0500 3, 18 -- Subject: microtome repair service 3, 18 -- X-Mailer: FirstClass 9.1 (build 9.207) 3, 18 -- X-FC-SERVER-TZ: 15729388 3, 18 -- To: microscopy-at-microscopy.com 3, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} 3, 18 -- MIME-Version: 1.0 3, 18 -- Content-Type: text/plain; charset=UTF-8 3, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Membrane thickness can be determined using the the inverse harmonic mean. It is not necessary to find places where the membrane is normal to the section. The inverse harmonic mean method determine the mean membrane thickness even when the membrane thickness is not constant.
Quoting gerd.leitinger-at-meduni-graz.at:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear List, } } I have been asked whether it is possible to determine the thickness of } biomembranes in TEM samples. } Any suggestions as to how to proceed? } } Cryo-methods are not available at the moment, although we might get access to } a high pressure cryo fixer in the future. } } thank you } } Gerd } } } } } -- } Dr. Gerd Leitinger } } Laboratory Coordinator } Core Facility Ultrastructure Analysis } Center for Medical Research (ZMF) } Medical University of Graz } } Postal address: } Institute of Cell Biology, Histology and Embryology } Harrachgasse 21 } 8010 Graz } Austria } Tel. +43 316 380 4237 } Fax. +43 316 380 9625 } Mailto: Gerd.Leitinger-at-meduni-graz.at } } } } } ==============================Original Headers============================== } 12, 22 -- From gerd.leitinger-at-meduni-graz.at Thu Nov 6 09:31:57 2008 } 12, 22 -- Received: from si069.meduni-graz.at (si082.meduni-graz.at } [193.170.105.82]) } 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mA6FVuAQ013751 } 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Nov 2008 09:31:57 -0600 } 12, 22 -- X-AuditID: 0ac80c45-a8239bb000003404-9a-49130dec0018 } 12, 22 -- Received: from si062.meduni-graz.at (si062.meduni-graz.at } [10.200.12.62]) } 12, 22 -- by si069.meduni-graz.at (Symantec Mail Security) with ESMTP id } 837F04DC002 } 12, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Nov 2008 16:31:56 +0100 } (CET) } 12, 22 -- Received: from connect_62-MTA by si062.meduni-graz.at } 12, 22 -- with Novell_GroupWise; Thu, 06 Nov 2008 16:31:56 +0100 } 12, 22 -- Message-Id: {49131B9E.E434.00B0.0-at-meduni-graz.at} } 12, 22 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 } 12, 22 -- Date: Thu, 06 Nov 2008 16:31:24 +0100 } 12, 22 -- From: "Gerd Leitinger" {gerd.leitinger-at-meduni-graz.at} } 12, 22 -- To: {Microscopy-at-microscopy.com} } 12, 22 -- Subject: EM: determining relative membrane thickness } 12, 22 -- Mime-Version: 1.0 } 12, 22 -- Content-Type: text/plain; charset=US-ASCII } 12, 22 -- Content-Disposition: inline } 12, 22 -- X-Brightmail-Tracker: AAAAAA== } 12, 22 -- Content-Transfer-Encoding: 8bit } 12, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mA6FVuAQ013751 } ==============================End of - Headers============================== }
Robert Boehringer MS student Virginia Tech
==============================Original Headers============================== 5, 30 -- From rboehrin-at-vt.edu Mon Nov 10 22:53:09 2008 5, 30 -- Received: from lennier.cc.vt.edu (lennier.cc.vt.edu [198.82.162.213]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAB4r8BZ016505 5, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Nov 2008 22:53:09 -0600 5, 30 -- Received: from vivi.cc.vt.edu (IDENT:mirapoint-at-evil-vivi.cc.vt.edu [10.1.1.12]) 5, 30 -- by lennier.cc.vt.edu (8.12.11.20060308/8.12.11) with ESMTP id mAB4r7th026765 5, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Nov 2008 23:53:07 -0500 5, 30 -- Received: from imp-b08.cc.vt.edu (imp.cc.vt.edu [198.82.161.55]) 5, 30 -- by vivi.cc.vt.edu (MOS 3.10.2-GA) 5, 30 -- with ESMTP id KEO79905; 5, 30 -- Mon, 10 Nov 2008 23:53:03 -0500 (EST) 5, 30 -- Received: from imp-b08.cc.vt.edu (localhost.localdomain [127.0.0.1]) 5, 30 -- by imp-b08.cc.vt.edu (8.13.1/8.13.1) with ESMTP id mAB4r08i025703 5, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Nov 2008 23:53:00 -0500 5, 30 -- Received: (from apache-at-localhost) 5, 30 -- by imp-b08.cc.vt.edu (8.13.1/8.13.1/Submit) id mAB4qxY9025702 5, 30 -- for Microscopy-at-microscopy.com; Mon, 10 Nov 2008 23:52:59 -0500 5, 30 -- Received: from pool-71-254-68-3.ronkva.east.verizon.net (pool-71-254-68-3.ronkva.east.verizon.net [71.254.68.3]) 5, 30 -- by webmail.vt.edu (IMP) with HTTP; Mon, 10 Nov 2008 23:52:59 -0500 5, 30 -- Message-ID: {1226379179.49190fab8e32c-at-webmail.vt.edu} 5, 30 -- Date: Mon, 10 Nov 2008 23:52:59 -0500 5, 30 -- From: Robert Boehringer {rboehrin-at-vt.edu} 5, 30 -- To: Microscopy-at-microscopy.com 5, 30 -- Subject: Re: [Microscopy] EM: determining relative membrane thickness 5, 30 -- References: {200811061537.mA6FbOXq023298-at-ns.microscopy.com} 5, 30 -- In-Reply-To: {200811061537.mA6FbOXq023298-at-ns.microscopy.com} 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- User-Agent: Internet Messaging Program (IMP) 3.2.8 ==============================End of - Headers==============================
We are looking for a few used parts for our Leica UltraCut S microtomes (trimming block, trimming holder, .......). If anyone out there is trying to get rid of an old unit and would not mind to give away parts, please contact me off-line. Thank you very much in advance.
Hong Emory EM (404) 712-8491
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==============================Original Headers============================== 7, 32 -- From hyi-at-emory.edu Mon Nov 10 23:37:38 2008 7, 32 -- Received: from mr3.cc.emory.edu (mr3.cc.emory.edu [170.140.52.92]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAB5bbZX030751 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Nov 2008 23:37:38 -0600 7, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 32 -- by mr3.cc.emory.edu (8.13.1/8.13.1) with ESMTP id mAB5bVlq021325 7, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Nov 2008 00:37:32 -0500 7, 32 -- Received: from EXCHHUB1.Enterprise.emory.net (170.140.30.53) by 7, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server 7, 32 -- (TLS) id 8.1.311.2; Tue, 11 Nov 2008 00:37:24 -0500 7, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 7, 32 -- EXCHHUB1.Enterprise.emory.net ([170.140.30.53]) with mapi; Tue, 11 Nov 2008 7, 32 -- 00:37:31 -0500 7, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Date: Tue, 11 Nov 2008 00:37:30 -0500 7, 32 -- Subject: Microtome parts 7, 32 -- Thread-Topic: Microtome parts 7, 32 -- Thread-Index: AQHJQ7+WbiyXFrLmQkq5e19LThY5Hw== 7, 32 -- Message-ID: {3940C1839888A54DAE85AE20F294FB69010CBA1C6906-at-EXCHANGE12.Enterprise.emory.net} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- acceptlanguage: en-US 7, 32 -- Content-Type: text/plain; charset="us-ascii" 7, 32 -- MIME-Version: 1.0 7, 32 -- X-emory.edu-MailScanner: Found to be clean 7, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu 7, 32 -- X-Spam-Status: No 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAB5bbZX030751 ==============================End of - Headers==============================
I am desperately trying to wet my diamond knife (Diatome) both soaking it in detergent or wiping with styropor and alcohol. To no avail. Would you have a miraculous method to wet your knife? Any suggestion?
Best regards,
Stephane
==============================Original Headers============================== 7, 20 -- From nizets2-at-yahoo.com Tue Nov 11 07:57:53 2008 7, 20 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mABDvr7e003649 7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2008 07:57:53 -0600 7, 20 -- Received: (qmail 84931 invoked by uid 60001); 11 Nov 2008 13:57:52 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 20 -- b=Ic7mZ8U3K/x2CfgKg6IncTvvuyz73+aiaPm3+PJ0i04UkLDY2SWP1DxL2Y+XGg1Be4X7rn97Rt5skPE3SLNR07f8U6MlWCaUNTRh9O1Bkp9syri6xw+WUaUMTwZxnFLlfALZCBkMF/62YVkSEYJvTMfNVpnNnfQjCYLTTCnJkiw=; 7, 20 -- X-YMail-OSG: oy2u0S0VM1l7kGD.xGzznUHHqpm2GguDZ5ms1JXPC759XsmFCIqR9EtrUuYTsgC8hEfbKlr5FN0yO34hsp5B.fUrvAWqt1tIqlWP3au8dQtAMducAveqA9Q5XouOTcdr6Ljo 7, 20 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Tue, 11 Nov 2008 05:57:52 PST 7, 20 -- X-Mailer: YahooMailRC/1155.20 YahooMailWebService/0.7.260.1 7, 20 -- References: {200811071953.mA7Jr6Mb026745-at-ns.microscopy.com} 7, 20 -- Date: Tue, 11 Nov 2008 05:57:52 -0800 (PST) 7, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 20 -- Subject: Wetting the knife 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Message-ID: {806933.84813.qm-at-web37402.mail.mud.yahoo.com} ==============================End of - Headers==============================
Here is my most elegant solution: Spit on your finger. Take your eyelash stick (i.e., an eyelash attached to a thin wooden stick) and drag it through the protein-rich saliva. Gently wipe this on the downslope of the diamond knife edge. Viola!
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, November 11, 2008 7:59 AM To: Phillips, Thomas E.
Dear colleagues,
I am desperately trying to wet my diamond knife (Diatome) both soaking it in detergent or wiping with styropor and alcohol. To no avail. Would you have a miraculous method to wet your knife? Any suggestion?
Best regards,
Stephane
==============================Original Headers============================== 7, 20 -- From nizets2-at-yahoo.com Tue Nov 11 07:57:53 2008 7, 20 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mABDvr7e003649 7, 20 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2008 07:57:53 -0600 7, 20 -- Received: (qmail 84931 invoked by uid 60001); 11 Nov 2008 13:57:52 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:MIME-Ver sion:Content-Type:Message-ID; 7, 20 -- b=Ic7mZ8U3K/x2CfgKg6IncTvvuyz73+aiaPm3+PJ0i04UkLDY2SWP1DxL2Y+XGg1Be4X7rn 97Rt5skPE3SLNR07f8U6MlWCaUNTRh9O1Bkp9syri6xw+WUaUMTwZxnFLlfALZCBkMF/62YV kSEYJvTMfNVpnNnfQjCYLTTCnJkiw=; 7, 20 -- X-YMail-OSG: oy2u0S0VM1l7kGD.xGzznUHHqpm2GguDZ5ms1JXPC759XsmFCIqR9EtrUuYTsgC8hEfbKlr5 FN0yO34hsp5B.fUrvAWqt1tIqlWP3au8dQtAMducAveqA9Q5XouOTcdr6Ljo 7, 20 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Tue, 11 Nov 2008 05:57:52 PST 7, 20 -- X-Mailer: YahooMailRC/1155.20 YahooMailWebService/0.7.260.1 7, 20 -- References: {200811071953.mA7Jr6Mb026745-at-ns.microscopy.com} 7, 20 -- Date: Tue, 11 Nov 2008 05:57:52 -0800 (PST) 7, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 20 -- Subject: Wetting the knife 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Message-ID: {806933.84813.qm-at-web37402.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 20, 27 -- From PhillipsT-at-missouri.edu Tue Nov 11 08:24:06 2008 20, 27 -- Received: from mxtip01-missouri-out.um.umsystem.edu (mxtip01-missouri-out.um.umsystem.edu [209.106.229.45]) 20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mABEO51Y017991 20, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Nov 2008 08:24:06 -0600 20, 27 -- X-IronPort-Anti-Spam-Filtered: true 20, 27 -- X-IronPort-Anti-Spam-Result: ApoEABgkGUnRauUp/2dsb2JhbADCAQEJhm6ERgGCWX0 20, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 20, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 11 Nov 2008 08:24:05 -0600 20, 27 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 20, 27 -- Tue, 11 Nov 2008 08:24:05 -0600 20, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 27 -- Content-class: urn:content-classes:message 20, 27 -- MIME-Version: 1.0 20, 27 -- Content-Type: text/plain; 20, 27 -- charset="US-ASCII" 20, 27 -- Subject: [Microscopy] Wetting the knife 20, 27 -- Date: Tue, 11 Nov 2008 08:23:34 -0600 20, 27 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD054F82CC-at-UM-XMAIL06.um.umsystem.edu} 20, 27 -- X-MS-Has-Attach: 20, 27 -- X-MS-TNEF-Correlator: 20, 27 -- Thread-Topic: [Microscopy] Wetting the knife 20, 27 -- thread-index: AclEBZ9f18efiyBJQvCBh09zmQukzwAAiw2wAABQjPA= 20, 27 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 20, 27 -- To: {Microscopy-at-microscopy.com} 20, 27 -- X-OriginalArrivalTime: 11 Nov 2008 14:24:05.0402 (UTC) FILETIME=[26DA87A0:01C94409] 20, 27 -- Content-Transfer-Encoding: 8bit 20, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mABEO51Y017991 ==============================End of - Headers==============================
Stephane, Put it in a plasma, glow discharge. Should make it nicely hydrophillic. Tobias } } Dear colleagues, } } I am desperately trying to wet my diamond knife (Diatome) both } soaking it in detergent or wiping with styropor and alcohol. To no } avail. } Would you have a miraculous method to wet your knife? Any suggestion? } } Best regards, } } Stephane } }
A very weak electron lens causes pincushion distortion, viewed on the low side of the diffraction point. Moving slightly to the high side of the diffraction point you will see barrel distortion. Manufacturers balance these two distortions to make the image within the old fashioned "photo area" truly square. The result of balancing one lens with pincushion with another lens with barrel distortion is known as "S" distortion or "anisotropic" distortion. The image at the very edge of a large diameter viewing screen will display this distortion, its normal.
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com -----Original Message----- X-from: wadowska-at-upei.ca [mailto:wadowska-at-upei.ca] Sent: 28 October 2008 08:26 To: protrain-at-emcourses.com
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Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veerinary College/UPEI
Title-Subject: [Filtered] TEM image distortion
Question: Hello, I've noticed for some time now that image in our H7500 is distorted. When I look at the grid bar it has an "S" shape. The center is stright but the ends are slightly bend in oposite directions. It is visible at low to mid range magnifications. I am looking for advice what is the cause of that problem and how to correct it. TIA Dorota
That's a trick that I've been using for years....suggested to me by the wizards at Diatome. It does work beautifully, even if its got a high "yuck" factor. Lee
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-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Thank you for your question, always good to hear that the older Polaron models are still working. As for coating parameters, for gold and palladium, it is best to use voltage in the range of 2KV, with a current of around 20mA. For Pt, a finer grain metal, lower settings seem to help decrease the grain size, and 1 KV at 10 or 12mA can be ideally used.
For a manual, I'll send the Quorum Technologies/Polaron Manual to you under separate cover.
Regards,
Mike Dufraine EM-Product Manager Energy Beam Sciences, Inc.
Fred Hayes wrote: } Mike, } } I have a biorad/polaron sputter coater which I inherited and it still } works. Currently using it with a gold target. Model number is E5100 } Serial number 88107. } } Are you familiar with this model? I have no owners manual for it and } also have a Platinum and Palladium/Gold targets. } } Would the coat parameters be the same for all 3 targets? } } mdufraine-at-ebsciences.com wrote: } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Heather- } } } } Energy Beam Sciences Inc. is a distributor of the Polaron, and } } Emitech lines of SEM preparation equipment, including sputter coaters. } } } } Coaters come in a wide range of sizes and functions, from mini } } coaters that utilize noble metals for basic SEM coating, to large } } chamber, high vacuum } } systems that work with oxidizing and other specific metals. } } } } You may contact EBS, with specific questions, on the technical, and } } functional aspects on sputter coaters, and good luck on your search } } for a coater, } } as there are many good options you have to choose from. } } } } Mike Dufraine } } EM-Product Manager } } 800-992-9037 X340 } } } } } } heather.eberhardt-at-frx.com wrote: } } } } } ---------------------------------------------------------------------------- } } } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } } --------------------------------------------------------------------------- } } } } } } Remember this posting is most likely not from a Subscriber, so when } } } replying } } } please copy both heather.eberhardt-at-frx.com as well as the } } } MIcroscopy Listserver } } } --------------------------------------------------------------------------- } } } } } } } } } Email: heather.eberhardt-at-frx.com } } } Name: Heather Eberhardt } } } } } } Organization: Forest Research Institute } } } } } } Title-Subject: [Filtered] Sputter Coaters } } } } } } Question: Hi Fellow Listers, } } } I'm trying to get our first SEM approved through budget this year. } } } My application is drug particles, excipients and the like. (All } } } non-conducting materials.) I've been told I should also get a } } } sputter coater to help make life easier and was wondering if there } } } are any recommendations for ones you like in particular. Ease of } } } use is a priority, but low cost is also a good thing. } } } Thanks in advance, } } } Heather } } } } } } Login Host: 69.27.233.254 } } } --------------------------------------------------------------------------- } } } } } } } } } ==============================Original } } } Headers============================== } } } 6, 11 -- From zaluzec-at-microscopy.com Tue Nov 4 08:26:03 2008 } } } 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } } [206.69.208.22]) } } } 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id mA4EQ2jk030293 } } } 6, 11 -- for {microscopy-at-microscopy.com} ; Tue, 4 Nov 2008 } } } 08:26:03 -0600 } } } 6, 11 -- Mime-Version: 1.0 } } } 6, 11 -- Message-Id: {p06240809c5360be08c50-at-[206.69.208.22]} } } } 6, 11 -- Date: Tue, 4 Nov 2008 08:26:02 -0600 } } } 6, 11 -- To: microscopy-at-microscopy.com } } } 6, 11 -- From: heather.eberhardt-at-frx.com (by way of } } } MicroscopyListserver) } } } 6, 11 -- Subject: viaWWW: Sputter Coaters } } } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } } ==============================End of - } } } Headers============================== } } } } } } } }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 8, 21 -- From mdufraine-at-ebsciences.com Tue Nov 11 15:47:09 2008 8, 21 -- Received: from mail.ebsciences.com (mail.ebsciences.com [65.115.7.18]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mABLl8Px010296 8, 21 -- for {microscopy-at-microscopy.com} ; Tue, 11 Nov 2008 15:47:08 -0600 8, 21 -- Received: from [10.10.0.145] 8, 21 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 8, 21 -- (Exim 4.69) 8, 21 -- (envelope-from {mdufraine-at-ebsciences.com} ) 8, 21 -- id 1L014m-0002AL-CM; Tue, 11 Nov 2008 16:47:08 -0500 8, 21 -- Message-ID: {4919FD5C.3090504-at-ebsciences.com} 8, 21 -- Date: Tue, 11 Nov 2008 16:47:08 -0500 8, 21 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 8, 21 -- Organization: Energy Beam Sciences 8, 21 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 8, 21 -- MIME-Version: 1.0 8, 21 -- To: Fred Hayes {fahayes-at-ucdavis.edu} , microscopy-at-microscopy.com 8, 21 -- Subject: Re: [Microscopy] Re: viaWWW: Sputter Coaters 8, 21 -- References: {200811051455.mA5EtQ4i011941-at-ns.microscopy.com} {4918E294.7050401-at-ucdavis.edu} 8, 21 -- In-Reply-To: {4918E294.7050401-at-ucdavis.edu} 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am sure there is a plug-in for this, but I have been unable to locate it. I find lots of plug-ins dealing with stacks, but none seem right, unless I misunderstand.
I often have two images of the same object that I would like to overlay, merge, or whatever the proper term would be (I'm not sure). The images are close in magnification, and have slight differences in theta, or rotation. I am imagining a plug-in that perhaps allows three points to be selected in each image, representing identical points, and then one image placed over the other with the three points registered to each other, which would adjust the mag and theta to match. Being able to control the transparency of each would be nice, too. The images are totally different in appearance, so I don't think the stack plug-ins that align slices would work.
Does such a plug-in exist, or one that is close to doing this?
Thank you for your attention.
Darrell
==============================Original Headers============================== 6, 27 -- From milesd-at-us.ibm.com Tue Nov 11 17:43:06 2008 6, 27 -- Received: from e5.ny.us.ibm.com (e5.ny.us.ibm.com [32.97.182.145]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mABNh3l0003983 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Nov 2008 17:43:05 -0600 6, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 6, 27 -- by e5.ny.us.ibm.com (8.13.8/8.13.8) with ESMTP id mABNh3CU031606 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Nov 2008 18:43:03 -0500 6, 27 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 6, 27 -- by d01relay04.pok.ibm.com (8.13.8/8.13.8/NCO v9.1) with ESMTP id mABNh3Dr161312 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Nov 2008 18:43:03 -0500 6, 27 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 6, 27 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.13.3) with ESMTP id mABNh2hg021195 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Nov 2008 18:43:02 -0500 6, 27 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 6, 27 -- by d01av01.pok.ibm.com (8.12.11.20060308/8.12.11) with ESMTP id mABNh2xl021187 6, 27 -- for {Microscopy-at-Microscopy.Com} ; Tue, 11 Nov 2008 18:43:02 -0500 6, 27 -- To: Microscopy-at-Microscopy.Com 6, 27 -- MIME-Version: 1.0 6, 27 -- Subject: ImageJ query 6, 27 -- X-Mailer: Lotus Notes Release 7.0 HF277 June 21, 2006 6, 27 -- Message-ID: {OFBDAFDE18.65220F13-ON852574FE.00801C43-852574FE.0082472F-at-us.ibm.com} 6, 27 -- From: Darrell Miles {milesd-at-us.ibm.com} 6, 27 -- Date: Tue, 11 Nov 2008 18:43:03 -0500 6, 27 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.0.1|February 07, 2008) at 6, 27 -- 11/11/2008 18:43:05, 6, 27 -- Serialize complete at 11/11/2008 18:43:05 6, 27 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
I have a user who is using Image Pro Plus but can use another program, ImageJ, if need be.
She wants to distinguish between two size ranges of axons, let's say 50um and under and 51um and up, in a thick section of spinal cord (stained with toluidine blue). She's tried using Image Pro Plus for this but cannot find a function that can do this automatically and she must do several calculations by hand.
Is there any program out there that can count these axons and distinguish between the 2 sizes?
She's at her nerves end ;) and would like some fancy computer help.
Thanks in advance for any help you can send our way.
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 12, 25 -- From vapatpxs-at-yahoo.com Wed Nov 12 12:54:11 2008 12, 25 -- Received: from n76.bullet.mail.sp1.yahoo.com (n76.bullet.mail.sp1.yahoo.com [98.136.44.48]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mACIsA4G005261 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 12:54:10 -0600 12, 25 -- Received: from [216.252.122.217] by n76.bullet.mail.sp1.yahoo.com with NNFMP; 12 Nov 2008 18:54:10 -0000 12, 25 -- Received: from [69.147.84.88] by t2.bullet.sp1.yahoo.com with NNFMP; 12 Nov 2008 18:54:10 -0000 12, 25 -- Received: from [127.0.0.1] by omp204.mail.sp1.yahoo.com with NNFMP; 12 Nov 2008 18:54:10 -0000 12, 25 -- X-Yahoo-Newman-Property: ymail-5 12, 25 -- X-Yahoo-Newman-Id: 410358.3643.bm-at-omp204.mail.sp1.yahoo.com 12, 25 -- Received: (qmail 16223 invoked by uid 60001); 12 Nov 2008 18:54:10 -0000 12, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 25 -- s=s1024; d=yahoo.com; 12, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 12, 25 -- b=Tg5smNF/MgFRVgq0GTY0IhI2Xc73KOsrQ9ZfEZJl0lE19ZENVuP1hi6Q9w+rKzXJzg+RBI5hqND/v8rJpHSUlmA0BDgCQ9vPxiJZUoFe0xhDJRewgzGxBy6dW8luf8F+U0CxKxrhAVVUNzoBibUcV10zyyh0OxnoQGag+f3RCgk=; 12, 25 -- X-YMail-OSG: Js2AiUwVM1ng0A_YZ9CGYhNQxyv8YitAZ_3QJ3VDEV3ATr7M.OexdbwcakIZl0WR.dBal0WaUhJNSUtL502089c3KYgfvDDcW4Ow_ZlolWRU5ilcZtVizoQj6Z241MP06tK5ew-- 12, 25 -- Received: from [132.239.85.200] by web46106.mail.sp1.yahoo.com via HTTP; Wed, 12 Nov 2008 10:54:09 PST 12, 25 -- X-Mailer: YahooMailWebService/0.7.260.1 12, 25 -- Date: Wed, 12 Nov 2008 10:54:09 -0800 (PST) 12, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 12, 25 -- Reply-To: vapatpxs-at-yahoo.com 12, 25 -- Subject: Counting shapes 12, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 12, 25 -- MIME-Version: 1.0 12, 25 -- Content-Type: text/plain; charset=us-ascii 12, 25 -- Message-ID: {135165.15057.qm-at-web46106.mail.sp1.yahoo.com} ==============================End of - Headers==============================
The Astromaterials Research and Exploration Science Directorate (ARES) at NASA's Johnson Space Center (JSC) is seeking a team member with expertise in Scanning Electron Microscopy (SEM) to support ongoing research and analysis projects. ARES is responsible for the curation and analysis of a wide range of solar system materials including the Apollo lunar samples, a large meteorite collection, comet dust samples (Stardust mission samples), samples from the Sun (Genesis sample return mission), and space-exposed hardware. Ongoing research includes a wide range of planetary, meteoritical, and space exploration topics. ARES staff member backgrounds include geology, chemistry, astronomy, physics, plus biology, mathematics, computer science, and engineering.
We are searching for an individual with broad experience in scanning electron microscopy to assist and train researchers and students in the use of a field-emission SEM and a low-vacuum SEM instrument. Typical analyses include secondary electron imaging, backscattered electron imaging, low voltage work, and X-ray analysis and mapping using energy-dispersive x-ray spectrometry. Responsibilities include, but are not limited to: maintaining and operating all aspects of these microscopes, coordinating service for the instruments, and supporting peer-review research through high quality analyses of astromaterials. Secondary responsibilities may include development of new analytical techniques. In addition to the SEMs, the facility also houses state-of-the art transmission electron microscopes, and electron microprobe, and supporting instrumentation.
This is a team ESCG position with Jacobs Technology. A bachelor's degree from an accredited university is a minimum requirement; the degree should be in an applicable geoscience, materials science, or engineering field. An advanced degree with strong experience in one of these fields is highly preferred. Five years of relevant experience is preferred. The candidate should have a strong grasp of the theory and practice of scanning electron microscopy analysis and data reduction. Experience with field emission sources is a plus. Knowledge of geological and planetary mineralogy is a plus, as is the ability to interpret analyses in a geologically meaningful way. Good computer skills are essential. Because the employee will often be working with students, and others who may be unfamiliar with electron beam instruments, good people skills are required. Must meet eligibility requirements to receive and maintain a DoD security clearance (i.e., almost certainly needs to be a US citizen).
Houston is the fourth largest city in the United States. Its downtown skyline has been ranked one of the top ten in the world. Houston's economy has a broad industrial base in the energy, manufacturing, aeronautics, transportation, and health care sectors; only New York City is home to more Fortune 500 headquarters. The Port of Houston ranks first in the United States in international waterborne tonnage. Strong performance in energy sectors has fuelled the Houston economy in recent years and low housing costs have kept the ongoing housing slump largely at bay. The city has a multicultural population and hosts a museum district, a year-round theater district, the internationally-renowned Texas Medical Center, 337 parks, four public and three private universities, and a number of professional sports teams. It enjoys a subtropical climate and the nearby coastline is a haven for bird watching, sailing, and fishing.
To apply for this position, please go to: https://www.cytiva.com/jacobs/ext/detail.asp?jacobs4818
==============================Original Headers============================== 8, 30 -- From lindsay.p.keller-at-nasa.gov Wed Nov 12 15:02:40 2008 8, 30 -- Received: from ndjsnpf01.ndc.nasa.gov (ndjsnpf01.ndc.nasa.gov [198.117.1.121]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mACL2e84026310 8, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 15:02:40 -0600 8, 30 -- Received: from ndjsppt02.ndc.nasa.gov (ndjsppt02.ndc.nasa.gov [198.117.1.101]) 8, 30 -- by ndjsnpf01.ndc.nasa.gov (Postfix) with ESMTP id 69F2632814E 8, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 15:02:39 -0600 (CST) 8, 30 -- Received: from ndjsxgw04.ndc.nasa.gov (ndjsxgw04.ndc.nasa.gov [129.166.32.112]) 8, 30 -- by ndjsppt02.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id mACL2eOU014988 8, 30 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 15:02:40 -0600 8, 30 -- Received: from NDJSEVS21A.ndc.nasa.gov ([129.166.32.213]) by ndjsxgw04.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 8, 30 -- Wed, 12 Nov 2008 15:02:39 -0600 8, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 30 -- Content-class: urn:content-classes:message 8, 30 -- MIME-Version: 1.0 8, 30 -- Content-Type: text/plain; 8, 30 -- charset="US-ASCII" 8, 30 -- Disposition-Notification-To: "Keller, Lindsay P. (JSC-KR)" {Lindsay.P.Keller-at-nasa.gov} 8, 30 -- Subject: Position Available - Scanning Electron Microscopy 8, 30 -- Date: Wed, 12 Nov 2008 15:02:38 -0600 8, 30 -- Message-ID: {48550B57777F6F498C94B5D9FD11B11C01B4FFA4-at-NDJSEVS21A.ndc.nasa.gov} 8, 30 -- X-MS-Has-Attach: 8, 30 -- X-MS-TNEF-Correlator: 8, 30 -- Thread-Topic: Position Available - Scanning Electron Microscopy 8, 30 -- Thread-Index: AclFCf7QSuONyCt1S1aHOi7Y7BJJ5g== 8, 30 -- From: "Keller, Lindsay P. (JSC-KR)" {Lindsay.P.Keller-at-nasa.gov} 8, 30 -- To: {microscopy-at-microscopy.com} 8, 30 -- X-OriginalArrivalTime: 12 Nov 2008 21:02:39.0365 (UTC) FILETIME=[FF197350:01C94509] 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mACL2e84026310 ==============================End of - Headers==============================
Dear All- What image acquisition and anlysis packages are you guys using (that you are happy with)? A large number of us here have been MetaMorph users for many years, but the level of support from the new owners leaves much to be desired. It was always expensive to purchase, but now the costs of upgrading to the next level of software (even within the same release) have gotten astronomical. We need something that can drive the microscopes as well as handle intensive image analysis including 3D-4D reconstructions & 3D deconvolution as well as the usual things like thresholding, counting objects, etc. Please respond to me (off list, if you'd like) about the packages you are using. This info is for my Core as well as my Chariman's whole lab and will probably trickle down to other labs here. I've attended the short courses at M&M, so I have an idea where to start, but I am interested in feedback from "the gang" thanks, Lee -- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
For the purposes that you describe, I feel that one of the strongest software available is SlideBook from Intelligent Imaging Innovations. Extremely flexible for complex hardware control and acquisition as well as decon, visualization and analysis. Their team is also quite responsive for hardware and software support. I've been a customer for about 5 years and have returned to them for new instrumentation purchases a handful of times.
Feel free to contact me directly if you'd like to talk a bit more about the fit with your needs.
-- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office (901) 495-2536 Cell (901) 603-3162 samuel.connell-at-stjude.org
-----Original Message----- X-from: lcgould-at-med.cornell.edu [mailto:lcgould-at-med.cornell.edu] Sent: Wednesday, November 12, 2008 3:08 PM To: Connell, Samuel
Dear All- What image acquisition and anlysis packages are you guys using (that you are happy with)? A large number of us here have been MetaMorph users for many years, but the level of support from the new owners leaves much to be desired. It was always expensive to purchase, but now the costs of upgrading to the next level of software (even within the same release) have gotten astronomical. We need something that can drive the microscopes as well as handle intensive image analysis including 3D-4D reconstructions & 3D deconvolution as well as the usual things like thresholding, counting objects, etc. Please respond to me (off list, if you'd like) about the packages you are using. This info is for my Core as well as my Chariman's whole lab and will probably trickle down to other labs here. I've attended the short courses at M&M, so I have an idea where to start, but I am interested in feedback from "the gang" thanks, Lee -- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Dear List, When I make a FFT in ImageJ, I typically get both horizontal and vertical bright streaks crossing at the center. I know that these arise from intensity gradients across the image, and that filtering the image with a Hanning window--a cosine-weighted function that is 1 at the center and 0 around the edges--before taking the FFT will eliminate these streaks. Is there a function in ImageJ to apply to the image that will accomplish the same thing, or an operation on the FFT that I can use to remove the streaks after the FFT is produced? TIA. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Wed Nov 12 16:39:46 2008 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mACMdjjn004297 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 16:39:45 -0600 2, 20 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by fire-doxen-postvirus (Postfix) with ESMTP id 69D17328A6A 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 14:39:45 -0800 (PST) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 2, 20 -- by fire-doxen-ssl (Postfix) with ESMTP id 8F4B3328A61 2, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 14:39:44 -0800 (PST) 2, 20 -- Message-Id: {0D98C7D3-7B8B-4E30-B6B3-E1501251ADD2-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 2, 20 -- Subject: ImageJ question 2, 20 -- Date: Wed, 12 Nov 2008 14:39:44 -0800 2, 20 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
On Nov 12, 2008, at 2:56 PM, Ruben Diaz-Avalos wrote:
} I am quite interested in knowing if people have carried out } measurements of contamination rates for different microscopes, and } what are the results of their measurements, to find out how the } different instruments fare in this test. At the NYSBC we have both } JEOL and FEI instruments, and we measure significantly different } contamination rates between the two brands, therefore I would like } to know if we are observing a common trend. } } By contamination rate in this case, I mean the rate of accumulation } of water on the grid's surface per hour (in nanometers per hour). A } low contamination rate obviously is very desirable, since only then } a sample can be used for a long session of data collection.
Dear Ruben, When our Tecnai scopes were installed, the contamination rates were measured as a part of the acceptance tests. These rates were slower than the specified rates for both instruments, but I don't have access to the actual numbers at present. I have not seen significant build- up of contamination on the T12 during an entire day of observations-- about 8 to 10 hours--and I have been able to collect data for a few days in a row on the Polara at LN2 temperature, but there is observable contamination from N2 at LHe temperature within several hours. The N2 contamination can be removed by withdrawing the specimen into the insertion rod and leaving it at LN2 temperature for a few minutes. If the specimen is left in the column over the weekend at LN2 temperature, there will be noticeable contamination, but specimens left in the multi-specimen holder during that interval or longer and maintained at LN2 temperature look as good as fresh specimens when they are introduced into the column. I don't remember any of the users of the Polara finding that their specimens have become contaminated while sitting in the multi-specimen holder, so I cold not say what the maximum time until noticeable contamination might be. I also have not seen any observable contamination on the UEM (a TF20), where I am now working, but I have not had any sessions longer than about 8 hours. I was not working at the UEM when it was installed, so I don't know what the contamination rate was found to be, although I am pretty sure that it was measured during installation. I have no experience with JEOL instruments, so I cannot make any comparisons. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Nov 12 17:39:30 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mACNdUIa018839 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 17:39:30 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id E200F3289DE 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 15:39:29 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id CE423328A64 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 15:39:28 -0800 (PST) 6, 22 -- Message-Id: {354A5168-44BB-4498-A0E4-30F3C48C8AB5-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {20081112225656.691940bf-at-nysbc.org} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [3dem] Contamination rates 6, 22 -- Date: Wed, 12 Nov 2008 15:39:28 -0800 6, 22 -- References: {20081112225656.691940bf-at-nysbc.org} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I recently purchased a used Pelco 3450 laboratory microwave with the Pelco 3420 load cooler. I have the operation manuals, but no protocols for specific TEM applications. Does anyone know of on-line resources for the use of this model? Or procedures you have written up and would be willing to share?
Ralph Common Michigan State University Dept. of Physiology
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Email: bchan-at-microassembly.com Name: Brian Chan
Organization: microassembly
Title-Subject: [Filtered] JEOL 35C
Question: To All:
I was wondering if anyone knew the specs for this SEM? Like what was the resolution and capability of this unit? Thanks.
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Title-Subject: [Filtered] Reference image subtraction in photoshop
Question: Dear All,
I have some "dirt" in my images that I assume is due to a dirty camera chip. I've cleaned everything I can in the optical path, Kohlered the illumination, and I still have these "spots" (they're more like regions of darker shading) in every single image. I know there has to be a way to "Subtract" a reference image of this stuff (taken away from my sample) from my sample images. I have Photoshop CS. Can anyone tell me how to do this??
Normal disclaimers aside, try Photoshop rubber stamp. If the anomalies are small, this should do the trick. For more industrial strength applications, I'd use Fovea Pro.
gary g.
At 05:08 PM 11/12/2008, you wrote:
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==============================Original Headers============================== 8, 21 -- From gary-at-gaugler.com Wed Nov 12 19:17:21 2008 8, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAD1HLf3013685 8, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 19:17:21 -0600 8, 21 -- Message-Id: {200811130117.mAD1HLf3013685-at-ns.microscopy.com} 8, 21 -- Received: (qmail 526 invoked from network); 12 Nov 2008 17:01:03 -0800 8, 21 -- Received: by simscan 1.1.0 ppid: 520, pid: 521, t: 0.1150s 8, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 8, 21 -- by smtp2 with SMTP; 12 Nov 2008 17:01:03 -0800 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Wed, 12 Nov 2008 17:17:17 -0800 8, 21 -- To: dcrippen-at-buckinstitute.org 8, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 21 -- Subject: Re: [Microscopy] viaWWW: Reference image subtraction in 8, 21 -- photoshop 8, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 21 -- In-Reply-To: {200811130108.mAD18F5U023423-at-ns.microscopy.com} 8, 21 -- References: {200811130108.mAD18F5U023423-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Whatever you do, remember that you need to report it as part of your image acquisition or post-acquisition manipulation! Reference image subtraciton is preferable to using any Photoshop filter or tool (except brightness, contrast, and histogram stretching). The rubber stamp is especially nasty as it fundamentally alters your data.
Aloha, Tina
} Email: dcrippen-at-buckinstitute.org } Name: Danielle Crippen } } Organization: Buck Institute for Age Research } } Title-Subject: [Filtered] Reference image subtraction in photoshop } } Question: Dear All, } } I have some "dirt" in my images that I assume is due to a dirty } camera chip. I've cleaned everything I can in the optical path, } Kohlered the illumination, and I still have these "spots" (they're } more like regions of darker shading) in every single image. I know } there has to be a way to "Subtract" a reference image of this stuff } (taken away from my sample) from my sample images. I have Photoshop } CS. Can anyone tell me how to do this?? } } A thousand thanks!! } } danielle } } Login Host: 206.176.234.82 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Wed Nov 12 19:06:33 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAD16WHl016546 } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 19:06:32 -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c5412e091e12-at-[206.69.208.22]} } 9, 11 -- Date: Wed, 12 Nov 2008 19:06:31 -0600 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: dcrippen-at-buckinstitute.org (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Reference image subtraction in photoshop } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 21 -- From tina-at-pbrc.hawaii.edu Wed Nov 12 19:27:53 2008 6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAD1Rr1m027297 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 19:27:53 -0600 6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id mAD1RmGJ009771 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 6, 21 -- Wed, 12 Nov 2008 15:27:48 -1000 (HST) 6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id mAD1Rk6w009768; 6, 21 -- Wed, 12 Nov 2008 15:27:47 -1000 (HST) 6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 6, 21 -- Date: Wed, 12 Nov 2008 15:27:46 -1000 (HST) 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 21 -- X-Sender: tina-at-halia 6, 21 -- To: dcrippen-at-buckinstitute.org 6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] viaWWW: Reference image subtraction in photoshop 6, 21 -- In-Reply-To: {200811130107.mAD17aMQ020649-at-ns.microscopy.com} 6, 21 -- Message-ID: {Pine.GSO.4.21.0811121524480.8712-100000-at-halia} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
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==============================Original Headers============================== 9, 21 -- From gary-at-gaugler.com Wed Nov 12 20:30:40 2008 9, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAD2UeYR009325 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 20:30:40 -0600 9, 21 -- Message-Id: {200811130230.mAD2UeYR009325-at-ns.microscopy.com} 9, 21 -- Received: (qmail 5235 invoked from network); 12 Nov 2008 18:14:23 -0800 9, 21 -- Received: by simscan 1.1.0 ppid: 5231, pid: 5233, t: 0.1294s 9, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 21 -- by smtp2 with SMTP; 12 Nov 2008 18:14:23 -0800 9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 21 -- Date: Wed, 12 Nov 2008 18:30:36 -0800 9, 21 -- To: tina-at-pbrc.hawaii.edu 9, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 21 -- Subject: Re: [Microscopy] Re: viaWWW: Reference image subtraction in 9, 21 -- photoshop 9, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 21 -- In-Reply-To: {200811130128.mAD1SuOY029694-at-ns.microscopy.com} 9, 21 -- References: {200811130128.mAD1SuOY029694-at-ns.microscopy.com} 9, 21 -- Mime-Version: 1.0 9, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
The rubber stamp (clone tool) works by replacing the image pixels with other pixels taken frowm elsewhere in the image.
If we consider each pixel of a micrograph (or other picture) taken for scientific purposes as a data point, replacing pixels with pixels from other pixels replaces data with (wrong) data.
Presumably getting rid of a constant set of pixels (a blob on the camera, for example) from an image by subtracting a background image will let any pixel value not subtracted by this method show through. Any pixel of a value that is subtracted as part of this manipulation will not show; hence the requirement that it be reported as an image manipulation.
This is still preferable to replacing "unwanted" pixels with pixels from somewhere else!
Get ready for the requirement that the "original" of any image that is published be available for inspection, and that all conditions of the acquisiiton of that image be stored with that image. It's coming.
I'm tired. If this doesn't make sense, I'll try again tomorrow.
Aloha, Tina
} How does rubber stamp pervert the data? } } Define this. } } gary g. } } } At 05:28 PM 11/12/2008, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Whatever you do, remember that you need to report it as part of your image } } acquisition or post-acquisition manipulation! Reference image subtraciton } } is preferable to using any Photoshop filter or tool (except brightness, } } contrast, and histogram stretching). The rubber stamp is especially nasty } } as it fundamentally alters your data. } } } } Aloha, } } Tina } } } } } } } Email: dcrippen-at-buckinstitute.org } } } Name: Danielle Crippen } } } } } } Organization: Buck Institute for Age Research } } } } } } Title-Subject: [Filtered] Reference image subtraction in photoshop } } } } } } Question: Dear All, } } } } } } I have some "dirt" in my images that I assume is due to a dirty } } } camera chip. I've cleaned everything I can in the optical path, } } } Kohlered the illumination, and I still have these "spots" (they're } } } more like regions of darker shading) in every single image. I know } } } there has to be a way to "Subtract" a reference image of this stuff } } } (taken away from my sample) from my sample images. I have Photoshop } } } CS. Can anyone tell me how to do this?? } } } } } } A thousand thanks!! } } } } } } danielle } } } } } } Login Host: 206.176.234.82 } } } --------------------------------------------------------------------------- } } } } } } ==============================Original } } Headers============================== } } } 9, 11 -- From zaluzec-at-microscopy.com Wed Nov 12 19:06:33 2008 } } } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mAD16WHl016546 } } } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 } } 19:06:32 -0600 } } } 9, 11 -- Mime-Version: 1.0 } } } 9, 11 -- Message-Id: {p06240801c5412e091e12-at-[206.69.208.22]} } } } 9, 11 -- Date: Wed, 12 Nov 2008 19:06:31 -0600 } } } 9, 11 -- To: microscopy-at-microscopy.com } } } 9, 11 -- From: dcrippen-at-buckinstitute.org (by way of MicroscopyListserver) } } } 9, 11 -- Subject: viaWWW: Reference image subtraction in photoshop } } } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } } ==============================End of - } } Headers============================== } } } } } } } **************************************************************************** } } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } } * Biological Electron Microscope Facility * (808) 956-6251 * } } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } } **************************************************************************** } } } } } } ==============================Original Headers============================== } } 6, 21 -- From tina-at-pbrc.hawaii.edu Wed Nov 12 19:27:53 2008 } } 6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu } } [128.171.22.7]) } } 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mAD1Rr1m027297 } } 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 } } 19:27:53 -0600 } } 6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } } 6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with } } ESMTP id mAD1RmGJ009771 } } 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } } bits=256 verify=NO); } } 6, 21 -- Wed, 12 Nov 2008 15:27:48 -1000 (HST) } } 6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu } } (8.12.11/8.12.11/Submit) with ESMTP id mAD1Rk6w009768; } } 6, 21 -- Wed, 12 Nov 2008 15:27:47 -1000 (HST) } } 6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned } } process doing -bs } } 6, 21 -- Date: Wed, 12 Nov 2008 15:27:46 -1000 (HST) } } 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } } 6, 21 -- X-Sender: tina-at-halia } } 6, 21 -- To: dcrippen-at-buckinstitute.org } } 6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} } } 6, 21 -- Subject: Re: [Microscopy] viaWWW: Reference image } } subtraction in photoshop } } 6, 21 -- In-Reply-To: {200811130107.mAD17aMQ020649-at-ns.microscopy.com} } } 6, 21 -- Message-ID: {Pine.GSO.4.21.0811121524480.8712-100000-at-halia} } } 6, 21 -- MIME-Version: 1.0 } } 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 10, 21 -- From tina-at-pbrc.hawaii.edu Wed Nov 12 21:08:57 2008 10, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAD38vCk023258 10, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 21:08:57 -0600 10, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 10, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id mAD38riH010065 10, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 10, 21 -- Wed, 12 Nov 2008 17:08:53 -1000 (HST) 10, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id mAD38pnU010061; 10, 21 -- Wed, 12 Nov 2008 17:08:52 -1000 (HST) 10, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 10, 21 -- Date: Wed, 12 Nov 2008 17:08:51 -1000 (HST) 10, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 10, 21 -- X-Sender: tina-at-halia 10, 21 -- To: Gary Gaugler {gary-at-gaugler.com} 10, 21 -- cc: MSA listserver {microscopy-at-microscopy.com} 10, 21 -- Subject: Re: [Microscopy] Re: viaWWW: Reference image subtraction in photoshop 10, 21 -- In-Reply-To: {200811130230.mAD2UeRt012931-at-sf-v240.pbrc.hawaii.edu} 10, 21 -- Message-ID: {Pine.GSO.4.21.0811121700150.9782-100000-at-halia} 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I've not done it with EM data for standard photo manipulation I convert the ref image to negative and simply overlay - objects that appear in both images then magically vanish.
-----Original Message----- X-from: dcrippen-at-buckinstitute.org [mailto:dcrippen-at-buckinstitute.org] Sent: 13 November 2008 01:14 To: Portman, Ian
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both dcrippen-at-buckinstitute.org as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] Reference image subtraction in photoshop
Question: Dear All,
I have some "dirt" in my images that I assume is due to a dirty camera chip. I've cleaned everything I can in the optical path, Kohlered the illumination, and I still have these "spots" (they're more like regions of darker shading) in every single image. I know there has to be a way to "Subtract" a reference image of this stuff (taken away from my sample) from my sample images. I have Photoshop CS. Can anyone tell me how to do this??
I agree with Tina, The rubber stamp or the cloning tool is great for repairing old photos of your grandparents or removing graffiti from the wall behind your children's vacation snap shots. But it is a replacement of data. Sometimes the removal of data is acceptable and justified, but it needs to be documented.
Of course these types of alteration should only be done on copies of images. The original should be maintained should you need to demonstrate the original and the degree of alteration.
My current employer is terrified of Photoshop because of it's data alteration abilities. Clearly this is more of a trust issue than simple data manipulation.
Frank Karl Linked-In Microscopy Group Manager
tina-at-pbrc.hawaii. edu To 11/12/2008 10:14 frank_karl-at-lincolnelectric.com PM cc
Subject Please respond to [Microscopy] viaWWW: Reference tina-at-pbrc.hawaii. image subtraction in photoshop edu
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
The rubber stamp (clone tool) works by replacing the image pixels with other pixels taken frowm elsewhere in the image.
If we consider each pixel of a micrograph (or other picture) taken for scientific purposes as a data point, replacing pixels with pixels from other pixels replaces data with (wrong) data.
Presumably getting rid of a constant set of pixels (a blob on the camera, for example) from an image by subtracting a background image will let any pixel value not subtracted by this method show through. Any pixel of a value that is subtracted as part of this manipulation will not show; hence the requirement that it be reported as an image manipulation.
This is still preferable to replacing "unwanted" pixels with pixels from somewhere else!
Get ready for the requirement that the "original" of any image that is published be available for inspection, and that all conditions of the acquisiiton of that image be stored with that image. It's coming.
I'm tired. If this doesn't make sense, I'll try again tomorrow.
Aloha, Tina
} How does rubber stamp pervert the data? } } Define this. } } gary g. } } } At 05:28 PM 11/12/2008, you wrote: } } } } } ----------------------------------------------------------------------------
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} } } } Whatever you do, remember that you need to report it as part of your image } } acquisition or post-acquisition manipulation! Reference image subtraciton } } is preferable to using any Photoshop filter or tool (except brightness, } } contrast, and histogram stretching). The rubber stamp is especially nasty } } as it fundamentally alters your data. } } } } Aloha, } } Tina } } } } } } } Email: dcrippen-at-buckinstitute.org } } } Name: Danielle Crippen } } } } } } Organization: Buck Institute for Age Research } } } } } } Title-Subject: [Filtered] Reference image subtraction in photoshop } } } } } } Question: Dear All, } } } } } } I have some "dirt" in my images that I assume is due to a dirty } } } camera chip. I've cleaned everything I can in the optical path, } } } Kohlered the illumination, and I still have these "spots" (they're } } } more like regions of darker shading) in every single image. I know } } } there has to be a way to "Subtract" a reference image of this stuff } } } (taken away from my sample) from my sample images. I have Photoshop } } } CS. Can anyone tell me how to do this?? } } } } } } A thousand thanks!! } } } } } } danielle } } } } } } Login Host: 206.176.234.82 } } } --------------------------------------------------------------------------- } } } } } } ==============================Original } } Headers============================== } } } 9, 11 -- From zaluzec-at-microscopy.com Wed Nov 12 19:06:33 2008 } } } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } } } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mAD16WHl016546 } } } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 } } 19:06:32 -0600 } } } 9, 11 -- Mime-Version: 1.0 } } } 9, 11 -- Message-Id: {p06240801c5412e091e12-at-[206.69.208.22]} } } } 9, 11 -- Date: Wed, 12 Nov 2008 19:06:31 -0600 } } } 9, 11 -- To: microscopy-at-microscopy.com } } } 9, 11 -- From: dcrippen-at-buckinstitute.org (by way of MicroscopyListserver) } } } 9, 11 -- Subject: viaWWW: Reference image subtraction in photoshop } } } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } } ==============================End of - } } Headers============================== } } } } } } } ****************************************************************************
} } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } } * Biological Electron Microscope Facility * (808) 956-6251 * } } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } } ****************************************************************************
} } } } } } ==============================Original Headers============================== } } 6, 21 -- From tina-at-pbrc.hawaii.edu Wed Nov 12 19:27:53 2008 } } 6, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu } } [128.171.22.7]) } } 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mAD1Rr1m027297 } } 6, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 } } 19:27:53 -0600 } } 6, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) } } 6, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with } } ESMTP id mAD1RmGJ009771 } } 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA } } bits=256 verify=NO); } } 6, 21 -- Wed, 12 Nov 2008 15:27:48 -1000 (HST) } } 6, 21 -- Received: from localhost by halia.pbrc.hawaii.edu } } (8.12.11/8.12.11/Submit) with ESMTP id mAD1Rk6w009768; } } 6, 21 -- Wed, 12 Nov 2008 15:27:47 -1000 (HST) } } 6, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned } } process doing -bs } } 6, 21 -- Date: Wed, 12 Nov 2008 15:27:46 -1000 (HST) } } 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } } 6, 21 -- X-Sender: tina-at-halia } } 6, 21 -- To: dcrippen-at-buckinstitute.org } } 6, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} } } 6, 21 -- Subject: Re: [Microscopy] viaWWW: Reference image } } subtraction in photoshop } } 6, 21 -- In-Reply-To: {200811130107.mAD17aMQ020649-at-ns.microscopy.com} } } 6, 21 -- Message-ID: {Pine.GSO.4.21.0811121524480.8712-100000-at-halia} } } 6, 21 -- MIME-Version: 1.0 } } 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII } } ==============================End of - Headers============================== }
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 10, 21 -- From tina-at-pbrc.hawaii.edu Wed Nov 12 21:08:57 2008 10, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAD38vCk023258 10, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 21:08:57 -0600 10, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 10, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id mAD38riH010065 10, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 10, 21 -- Wed, 12 Nov 2008 17:08:53 -1000 (HST) 10, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id mAD38pnU010061; 10, 21 -- Wed, 12 Nov 2008 17:08:52 -1000 (HST) 10, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 10, 21 -- Date: Wed, 12 Nov 2008 17:08:51 -1000 (HST) 10, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 10, 21 -- X-Sender: tina-at-halia 10, 21 -- To: Gary Gaugler {gary-at-gaugler.com} 10, 21 -- cc: MSA listserver {microscopy-at-microscopy.com} 10, 21 -- Subject: Re: [Microscopy] Re: viaWWW: Reference image subtraction in photoshop 10, 21 -- In-Reply-To: {200811130230.mAD2UeRt012931-at-sf-v240.pbrc.hawaii.edu} 10, 21 -- Message-ID: {Pine.GSO.4.21.0811121700150.9782-100000-at-halia} 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
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'Dirt' on a camera chip is normally fairly in focus. I am assuming you can't simply remove the camera and clean the 'chip' [normally a cover-slip thickness piece of glass covering the actual chip on dedicated microscope cameras]. This is a delicate procedure and I use ether and pure cotton wool on a stick and clean in a circular motion so as not to drag dust in from the edge of the fitting. Canister Air jets can be used but I mostly use a large hand puffer after getting nasty propellant sludge all over this delicate glass when using an 'invertible' duster spray many years ago. If it’s a camera that takes a lens [i.e. SLR or compact] take some pictures away from the microscope to check for dust. If the camera's a £12k Hamamatsu get in an engineer if you're really worried about doing it yourself [his insurance can cover it], cost about £300 to £600, full microscope cleaning included. My advice is to rotate any optics that can rotate [like cameras, filters & objectives], and if the dust rotates with it then you have found the problem.
Normally however 'dust shadows on an image' is because the condenser aperture diaphragm iris [the one that isn't the condenser field diaphragm iris that’s adjusted for Koehler illumination] has been closed down which decreases resolution/brightness but increases contrast and depth of field. Increases in the latter naturally brings in all the dust on the microscope internal optics producing an image identical to the one you describe [dusty shadows all over the place]. A dark shadow in the centre could be badly adjusted Koehler illumination, and here the phase rings are the culprit. There's plenty more other possibilities, particularly with oil immersion, but the above is the most likely. Hopefully it's not due to damaged optics.
The photograph
Removing dirt from the image via Photoshop is pointless really as you can't recover the information under the shadows [unless you have another identical picture of the sample with the dust in different place]. All you can do otherwise is copy and paste similar looking areas nearby over the top of the muck. I would do that for photos of the kids faces and for web site images but not for scientific publication as the information you are adding is false. My favourite tools are the lasso and magic wand, with copy, drag and paste over, with a hint of smudge over any obvious borders after the layers are merged. But this is mostly art & aesthetics not science.
As you say, you can 'background subtract' an image of an empty field from the specimen image you have just captured [using the identical optics/light intensity settings]. This markedly reduces the effect of uneven illumination [say if you are using a 1x objective with no condenser] but it will not remove dark black dust shadows if they have obscured the specimen detail. Naturally you have to do this live when you are taking the photos [it can't be done days later unless the empty field 'subtract' image was also saved]. Our image acquisition software does this 'background subtract' automatically for us [with varying degrees of success] so I don't really have to think about how it does it on a pixel to pixel level. With gradual uneven lighting you can often artificially generate your own background subtract image, but this won't work for your 'dust' shadows. The Molecular Expressions website has some info on this:
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: dcrippen-at-buckinstitute.org [mailto:dcrippen-at-buckinstitute.org] Sent: 13 November 2008 01:13 To: kjmorris-at-well.ox.ac.uk
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Title-Subject: [Filtered] Reference image subtraction in photoshop
Question: Dear All,
I have some "dirt" in my images that I assume is due to a dirty camera chip. I've cleaned everything I can in the optical path, Kohlered the illumination, and I still have these "spots" (they're more like regions of darker shading) in every single image. I know there has to be a way to "Subtract" a reference image of this stuff (taken away from my sample) from my sample images. I have Photoshop CS. Can anyone tell me how to do this??
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dstumpf-at-hersheys.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dstumpf-at-hersheys.com Name: Dave Stumpf
Organization: The Hershey Company
Title-Subject: [Filtered] JEOL 35C
Question: I'm looking at the original price quote for the 35CF I bought in 1981, and the guaranteed resolution was 60A.
I believe Ted Pella's website has a whole section of microwave protocols. You can see our generic microwave protocol at http://www.emc.missouri.edu/Pdfs/General%202-ME%20Microwave%20Processing %20Protocol.pdf.
Good luck, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: rcommon-at-msu.edu [mailto:rcommon-at-msu.edu] Sent: Wednesday, November 12, 2008 7:02 PM To: Tindall, Randy D.
I recently purchased a used Pelco 3450 laboratory microwave with the Pelco 3420 load cooler. I have the operation manuals, but no protocols for specific TEM applications. Does anyone know of on-line resources for the use of this model? Or procedures you have written up and would be willing to share?
Ralph Common Michigan State University Dept. of Physiology
==============================Original Headers============================== 3, 24 -- From rcommon-at-msu.edu Wed Nov 12 19:01:01 2008 3, 24 -- Received: from sys14.mail.msu.edu (sys14.mail.msu.edu [35.9.75.114]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAD111EL005463 3, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Nov 2008 19:01:01 -0600 3, 24 -- Received: from [35.9.122.125] (helo=emlab) 3, 24 -- by sys14.mail.msu.edu with esmtpsa (Exim 4.63 #1) 3, 24 -- (TLSv1:RC4-MD5:128) 3, 24 -- id 1L0QZx-0003e0-GW 3, 24 -- for Microscopy-at-microscopy.com; Wed, 12 Nov 2008 20:01:01 -0500 3, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 3, 24 -- To: {Microscopy-at-microscopy.com} 3, 24 -- Subject: Pelco 3450 microwave 3, 24 -- Date: Wed, 12 Nov 2008 20:01:15 -0500 3, 24 -- Message-ID: {000601c9452b$54d78310$7d7a0923-at-msu.edu} 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="iso-8859-1" 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 (Normal) 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1933 3, 24 -- Importance: Normal 3, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
==============================Original Headers============================== 12, 30 -- From TindallR-at-missouri.edu Thu Nov 13 12:18:33 2008 12, 30 -- Received: from mxtip01-missouri-out.um.umsystem.edu (mxtip01-missouri-out.um.umsystem.edu [209.106.229.45]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mADIIWWS028812 12, 30 -- for {microscopy-at-microscopy.com} ; Thu, 13 Nov 2008 12:18:33 -0600 12, 30 -- X-IronPort-Anti-Spam-Filtered: true 12, 30 -- X-IronPort-Anti-Spam-Result: ApoEACf+G0nRauUo/2dsb2JhbADHIQEJhkqEU4JZfoMy 12, 30 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 12, 30 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 13 Nov 2008 12:18:32 -0600 12, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 12, 30 -- Thu, 13 Nov 2008 12:18:32 -0600 12, 30 -- x-mimeole: Produced By Microsoft Exchange V6.5 12, 30 -- Content-class: urn:content-classes:message 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; 12, 30 -- charset="us-ascii" 12, 30 -- Subject: RE: [Microscopy] Pelco 3450 microwave 12, 30 -- Date: Thu, 13 Nov 2008 12:18:28 -0600 12, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7B9E-at-UM-XMAIL08.um.umsystem.edu} 12, 30 -- In-Reply-To: {200811130102.mAD125qA007375-at-ns.microscopy.com} 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- Thread-Topic: [Microscopy] Pelco 3450 microwave 12, 30 -- Thread-Index: AclFK3JHpXo0EHXMTZ2MDXjptQ+W5AAkH8/g 12, 30 -- References: {200811130102.mAD125qA007375-at-ns.microscopy.com} 12, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 12, 30 -- To: {rcommon-at-msu.edu} 12, 30 -- Cc: {microscopy-at-microscopy.com} 12, 30 -- X-OriginalArrivalTime: 13 Nov 2008 18:18:32.0406 (UTC) FILETIME=[3C447F60:01C945BC] 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mADIIWWS028812 ==============================End of - Headers==============================
On Nov 13, 2008, at 5:59 AM, kjmorris-at-well.ox.ac.uk wrote:
} I have some "dirt" in my images that I assume is due to a dirty } camera chip. I've cleaned everything I can in the optical path, } Kohlered the illumination, and I still have these "spots" (they're } more like regions of darker shading) in every single image. I know } there has to be a way to "Subtract" a reference image of this stuff } (taken away from my sample) from my sample images. I have Photoshop } CS. Can anyone tell me how to do this??
} } Removing dirt from the image via Photoshop is pointless really as } } you can't } } recover the information under the shadows [unless you have another } } identical } } picture of the sample with the dust in different place]. } Dear Danielle, Not all "dirt" is 100% opaque, so reference images of a blank field with illumination off for one and on for another can give you both a dark reference and a bright reference that can be used to subtract noise and normalize the response of each pixel. DigitalMicrograph (usual disclaimer; no commercial interest, just a user) provides for this in their software, and I assume that there is an equivalent function is whatever software you use. If the shadows are not too strong, normalizing is acceptable--with appropriate documentation, as mentioned by others--but if the shadows are too dark, the signal/noise ratio may be inadequate in the affected pixels. (In the limit of completely opaque dirt the normalization will multiply the pixel values by a very large number, or will replace the pixel value with a constant, depending on how the normalization program has been written.) Another method, which may not work if you are taking images of a dynamic process, is to take two images, one of which has the specimen offset somewhat with respect to the other, then you should have the correct intensity available in one of the two images, so you can cut and paste the appropriate areas. Again, save the originals and document the processing. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Thu Nov 13 12:28:55 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mADIStNm009962 6, 22 -- for {microscopy-at-microscopy.com} ; Thu, 13 Nov 2008 12:28:55 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 5C6622E50C02; 6, 22 -- Thu, 13 Nov 2008 10:28:55 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-215.caltech.edu (DHCP-19-215.caltech.edu [131.215.19.215]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 4E2032E50BDE; 6, 22 -- Thu, 13 Nov 2008 10:28:54 -0800 (PST) 6, 22 -- Message-Id: {2A762145-B1DF-459B-B2E0-ECC17002BE4E-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: dcrippen-at-buckinstitute.org, microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200811131359.mADDxZkY027599-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] RE: viaWWW: Reference image subtraction in photoshop 6, 22 -- Date: Thu, 13 Nov 2008 10:28:54 -0800 6, 22 -- References: {200811131359.mADDxZkY027599-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Thanks for the feedback everyone. There is a JEOL 35CF for sale online on ebay that looks OK. Does anyone know anything particular details about this system? Does anyone know how does the Hitachi S-510 compare to the JEOL 35CF? The company actually has a S-510 sitting in the lab, but not functional. Jayma, please send those brochure. Thanks. Link to the JEOL is below.
I would like to draw your attention to a symposium being organized at M&M 2009 where the title and description are
Raw Data and Metadata: Comprehensive and Ethical Collection, Storage, Manipulation and Retrieval of Images.
We are in an era where it is becoming essential to record comprehensive meta-data along with our microscope images. Meta-data can be used for documenting experiments to make the data useful and meaningful to others if we are required to make raw data publicly available. We also need to be concerned with the ethics of post acquisition image manipulation - reliable, secure meta-data can help prevent misuse of images. Data mining is attracting attention with the advent of the "semantic web". We seek presentations and posters on all aspects of data collection, storage and retrieval especially in a core facility setting.
I would be delighted if the session was oversubscribed with contributions, and just as delighted if the room assigned to the symposium was overflowing with attendees!
I would like to strongly agree with the point that Tina made yesterday. Think of an image as a set of numbers - for a monochrome image this is simply an x coordinate, a y coordinate and an intensity value. ANYTHING that changes those numbers should be carefully considered and ALWAYS reported.
... but then of course we get into what was done to a signal before we, as users, even get to see it as an image....... hence the symposium and thoughts about metadata and what really is the definition of "raw data"
See you in Richmond for lively discussions.
Regards
Chris
Christopher J Gilpin Ph.D. Assistant Professor Director, Molecular and Cellular Imaging Facility K1.A04 UT Southwestern Medical Center at Dallas 5323 Harry Hines Boulevard Dallas, TX 75390-9039 Phone +1 214 648 2827 Fax +1 214 648 6408
==============================Original Headers============================== 22, 27 -- From christopher.gilpin-at-utsouthwestern.edu Thu Nov 13 14:42:28 2008 22, 27 -- Received: from zixvpm01.utsouthwestern.edu (zixvpm01.utsouthwestern.edu [199.242.236.196]) 22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mADKgSgv009269 22, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Nov 2008 14:42:28 -0600 22, 27 -- Received: from zixvpm01.utsouthwestern.edu (ZixVPM [127.0.0.1]) 22, 27 -- by Outbound.utsouthwestern.edu (Proprietary) with ESMTP id 73C1D2E810B 22, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Nov 2008 14:42:28 -0600 (CST) 22, 27 -- Received: from swlx167.swmed.edu (swlx167.swmed.edu [199.165.152.167]) 22, 27 -- by zixvpm01.utsouthwestern.edu (Proprietary) with ESMTP id A2646200053 22, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Nov 2008 14:42:25 -0600 (CST) 22, 27 -- Received: from [129.112.148.231] (helo=cgdesktop) 22, 27 -- by swlx167.swmed.edu with esmtp (Exim 4.44) 22, 27 -- id 1L0j1F-0003uS-9N 22, 27 -- for Microscopy-at-microscopy.com; Thu, 13 Nov 2008 14:42:25 -0600 22, 27 -- From: "Christopher Gilpin" {christopher.gilpin-at-utsouthwestern.edu} 22, 27 -- To: {Microscopy-at-microscopy.com} 22, 27 -- Date: Thu, 13 Nov 2008 14:42:28 -0600 22, 27 -- Message-ID: {000701c945d0$57afe870$070fb950$-at-gilpin-at-utsouthwestern.edu} 22, 27 -- MIME-Version: 1.0 22, 27 -- Content-Type: text/plain; 22, 27 -- charset="us-ascii" 22, 27 -- Content-Transfer-Encoding: 7bit 22, 27 -- X-Mailer: Microsoft Office Outlook 12.0 22, 27 -- Thread-Index: AclF0FeJKwmcIGybS2WIsQY34z71ig== 22, 27 -- Content-Language: en-us 22, 27 -- X-Scan-Signature: 24ba24bf0e121cdfe7aca8ef5735848a 22, 27 -- Subject: Reference image subtraction in photoshop ==============================End of - Headers==============================
Hi, I was wondering what is good SEM reference book(s) to have on hand? I would like one that talks about the analysis and measurements techniques. I would also like one that talks about the mechanical aspect of a SEM.
Thanks, Brian Chan MicroAssembly
==============================Original Headers============================== 2, 19 -- From bchan-at-microassembly.com Thu Nov 13 19:54:43 2008 2, 19 -- Received: from omr2.networksolutionsemail.com (omr2.networksolutionsemail.com [205.178.146.52]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAE1shb6029020 2, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Nov 2008 19:54:43 -0600 2, 19 -- Received: from mail.networksolutionsemail.com (ns-omr2.mgt.netsol.com [10.49.6.65]) 2, 19 -- by omr2.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id mAE1shWV030055 2, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Nov 2008 20:54:43 -0500 2, 19 -- Received: (qmail 19326 invoked by uid 78); 14 Nov 2008 01:54:43 -0000 2, 19 -- Received: from unknown (HELO ?192.168.1.114?) (bchan-at-microassembly.com-at-76.254.227.222) 2, 19 -- by ns-omr2.lb.hosting.dc2.netsol.com with SMTP; 14 Nov 2008 01:54:43 -0000 2, 19 -- Message-ID: {491CDA5C.90102-at-microassembly.com} 2, 19 -- Date: Thu, 13 Nov 2008 17:54:36 -0800 2, 19 -- From: Brian Chan {bchan-at-microassembly.com} 2, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 2, 19 -- MIME-Version: 1.0 2, 19 -- To: Microscopy-at-Microscopy.Com 2, 19 -- Subject: SEM Reference Book 2, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Brian: the 2 books I like are: 1. "Scanning Electron Microscopy and X-Ray Microanalysis", 2nd edition, by Goldstein, Newbury, Echlin, Joy, Romig, Lyman, Fiori, and Lifshin, published by Kluwer Academic/Plenum Publishers. I also have the 3rd edition but the 2nd edition is my favorite. 2. "Scanning Electron Microscopy--A Student's Handbook" by Postek, Howard, Johnson, and McMichael published by Ladd Research (and available on their website).
bchan-at-microassembly.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, I was wondering what is good SEM reference book(s) to have on hand? } I would like one that talks about the analysis and measurements } techniques. I would also like one that talks about the mechanical } aspect of a SEM. } } Thanks, } Brian Chan } MicroAssembly } } ==============================Original Headers============================== } 2, 19 -- From bchan-at-microassembly.com Thu Nov 13 19:54:43 2008 } 2, 19 -- Received: from omr2.networksolutionsemail.com (omr2.networksolutionsemail.com [205.178.146.52]) } 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAE1shb6029020 } 2, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Nov 2008 19:54:43 -0600 } 2, 19 -- Received: from mail.networksolutionsemail.com (ns-omr2.mgt.netsol.com [10.49.6.65]) } 2, 19 -- by omr2.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id mAE1shWV030055 } 2, 19 -- for {Microscopy-at-Microscopy.Com} ; Thu, 13 Nov 2008 20:54:43 -0500 } 2, 19 -- Received: (qmail 19326 invoked by uid 78); 14 Nov 2008 01:54:43 -0000 } 2, 19 -- Received: from unknown (HELO ?192.168.1.114?) (bchan-at-microassembly.com-at-76.254.227.222) } 2, 19 -- by ns-omr2.lb.hosting.dc2.netsol.com with SMTP; 14 Nov 2008 01:54:43 -0000 } 2, 19 -- Message-ID: {491CDA5C.90102-at-microassembly.com} } 2, 19 -- Date: Thu, 13 Nov 2008 17:54:36 -0800 } 2, 19 -- From: Brian Chan {bchan-at-microassembly.com} } 2, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) } 2, 19 -- MIME-Version: 1.0 } 2, 19 -- To: Microscopy-at-Microscopy.Com } 2, 19 -- Subject: SEM Reference Book } 2, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 2, 19 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } } }
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==============================Original Headers============================== 4, 22 -- From r-holdford-at-ti.com Thu Nov 13 21:45:31 2008 4, 22 -- Received: from devils.ext.ti.com (devils.ext.ti.com [198.47.26.153]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAE3jUYJ012123 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Nov 2008 21:45:30 -0600 4, 22 -- Received: from dlep36.itg.ti.com ([157.170.170.91]) 4, 22 -- by devils.ext.ti.com (8.13.7/8.13.7) with ESMTP id mAE3jMQr002871; 4, 22 -- Thu, 13 Nov 2008 21:45:27 -0600 4, 22 -- Received: from [156.117.248.174] (localhost [127.0.0.1]) 4, 22 -- by dlep36.itg.ti.com (8.13.8/8.13.8) with ESMTP id mAE3jMjr000924; 4, 22 -- Thu, 13 Nov 2008 21:45:22 -0600 (CST) 4, 22 -- Message-ID: {491CF452.5020504-at-ti.com} 4, 22 -- Date: Thu, 13 Nov 2008 21:45:22 -0600 4, 22 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 22 -- Organization: SC Packaging Development -- FA Development 4, 22 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 4, 22 -- MIME-Version: 1.0 4, 22 -- To: bchan-at-microassembly.com, MSA Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] SEM Reference Book 4, 22 -- References: {200811140154.mAE1swZ2029276-at-ns.microscopy.com} 4, 22 -- In-Reply-To: {200811140154.mAE1swZ2029276-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
YOUNGSTOWN STATE UNIVERSITY invites applications for the faculty position described below.
DEPARTMENT: Chem., Mech., or Elec. Engineering or Physics COLLEGE: Science, Technology, Engineering & Mathematics RANK: Assistant Professor (Tenure-track) SALARY: Commensurate with qualifications and experience
MINIMUM QUALIFICATIONS: Ph.D. in Materials Science/Engineering or related field.
DATE AVAILABLE: August 17, 2009
OTHER INFORMATION RELEVANT TO THIS POSITION: Tenure-track position available in chemical, mechanical, or electrical engineering or physics in support of our growing emphasis in materials science and engineering. The successful candidate will have prior experience in the characterization and/or development of advanced materials (ceramics, metals, and ceramic-metallic composites) utilizing TEM and SEM techniques, in support of a major state-funded project that will establish a new electron microscopy facility at YSU including TEM, SEM, and EM sample prep lab. Preference will be given to candidates who are experienced with manufacturing techniques for the production of ceramic and metallic materials, as well as utilizing EM equipment to perform and analyze current research on the relationships between material properties and process parameters. Establishing an externally funded research program involving undergraduate and graduate students is expected, as is collaborations with local industrial companies. Teaching responsibilities may include both upper and lower-division courses in the relevant department. The filling of this position will be contingent on available funding.
CLOSING DATE FOR APPLICATIONS: Review of applications will begin January 1, 2009, and continue until position is filled.
Applicants must send (1) a letter of interest, (2) a current vita with employment history and dates, (3) a copy of your transcript* documenting academic qualifications for this position, and (4) three references which include the names, addresses, phone numbers or e-mail addresses to:
Prof. Tim Wagner Department of Chemistry Youngstown State University One University Plaza Youngstown OH 44555-0001 Phone: (330) 941-1960 e-mail: trwagner-at-ysu.edu
*NOTE: Youngstown State University recognizes only credits and degrees awarded by regionally accredited post-secondary institutions in the United States or by equivalent foreign institutions; accredited institutions can be found at http://www.chea.org. Exceptions may be approved by the Provost. As a term and condition of appointment, an official transcript must be received by Human Resources prior to a contract being issued. Position finalists will be required to complete a formal application and to submit three letters of reference prior to an on-campus interview. The selected candidate will also be required to sign a release for an employment background check and credential verification.
YSU IS AN AFFIRMATIVE ACTION/EQUAL OPPORTUNITY EMPLOYER COMMITTED TO INCREASING THE DIVERSITY OF ITS FACULTY, STAFF AND STUDENTS.
Information regarding campus safety at YSU, mandated by the Clery Act, is available at the following YSU web site: http://www.ysu.edu/righttk.pdf or you may request a copy of "Your Right to Know" from the Office of Human Resources, 3rd Floor, Jones Hall, 330-941-3122.
Youngstown State University is a state-assisted, urban institution of higher education which primarily, but not exclusively, serves the students of northeastern Ohio and western Pennsylvania.
The University has an enrollment of approximately 13,200 (head-count) in a wide variety of programs ranging from 2-year associate degrees to the Doctor of Education degree.
Schools and colleges of Youngstown State University include:
College of Business Administration College of Education College of Fine and Performing Arts College of Health and Human Services College of Liberal Arts and Social Sciences College of Science, Technology, Engineering and Mathematics School of Graduate Studies and Research
The campus, located north of downtown Youngstown, is compact and carefully designed, with most of the major structures being new or recently renovated. The Physical Plant is valued at approximately $320 million.
The city of Youngstown is located between Cleveland and Pittsburgh. The Youngstown-Warren area has a population in excess of 500,000 and offers the usual amenities of urban living but few of the problems of the big city. All types of housing are available within a 30-minute drive of the campus, and housing costs in the Youngstown area are significantly lower than in most metropolitan areas.
_________________________________
Joseph Kulik Materials Research Institute Penn State University Telephone: 814-865-0344 (w) 814-237-1483 (h) e-mail: juk12-at-psu.edu _________________________________
==============================Original Headers============================== 25, 19 -- From juk12-at-psu.edu Fri Nov 14 09:53:28 2008 25, 19 -- Received: from echo.mri.psu.edu (echo.mri.psu.edu [146.186.179.249]) 25, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAEFrS4U019107 25, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 09:53:28 -0600 25, 19 -- X-Ninja-PIM: Scanned by Ninja 25, 19 -- X-Ninja-AttachmentFiltering: (no action) 25, 19 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 25, 19 -- Content-class: urn:content-classes:message 25, 19 -- MIME-Version: 1.0 25, 19 -- Content-Type: text/plain; charset="us-ascii" 25, 19 -- Subject: TEM/SEM Faculty Position Announcement 25, 19 -- Date: Fri, 14 Nov 2008 10:53:26 -0500 25, 19 -- Message-ID: {8C366DE9194F154380A8666A1D90468C0226B144-at-echo.mri.psu.edu} 25, 19 -- Thread-Topic: TEM/SEM Faculty Position Announcement 25, 19 -- Thread-Index: AclGcSHajlc+j+drTLeLWvMo8c/PTQ== 25, 19 -- From: "Joe Kulik" {juk12-at-psu.edu} 25, 19 -- To: {Microscopy-at-microscopy.com} 25, 19 -- Content-Transfer-Encoding: 8bit 25, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAEFrS4U019107 ==============================End of - Headers==============================
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Hello All,
Could any of you provide an user or an instruction manual of a Nikon Diaphot 300 inverted fluorescence microscope preferably as a pdf file? Thanks in advance.
Necat Yilmaz
==============================Original Headers============================== 5, 31 -- From nyilmaz-at-mersin.edu.tr Fri Nov 14 14:23:05 2008 5, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAEKN4n3006669 5, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 14:23:05 -0600 5, 31 -- Received: from localhost (localhost [127.0.0.1]) 5, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 912C42F7108 5, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 22:22:27 +0200 (EET) 5, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 5, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 5, 31 -- by localhost (mail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 5, 31 -- with ESMTP id 9VqmjwaeDpfd for {Microscopy-at-microscopy.com} ; 5, 31 -- Fri, 14 Nov 2008 22:22:23 +0200 (EET) 5, 31 -- Received: from nejat1 (unknown [78.163.52.71]) 5, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 777F32F70FE 5, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 22:22:23 +0200 (EET) 5, 31 -- Message-ID: {001601c94696$c6f00ff0$2101a8c0-at-nejat1} 5, 31 -- From: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 5, 31 -- To: "EM-Mail Group" {Microscopy-at-microscopy.com} 5, 31 -- Subject: Nikon Diaphot 300 5, 31 -- Date: Fri, 14 Nov 2008 22:22:55 +0200 5, 31 -- MIME-Version: 1.0 5, 31 -- Content-Type: text/plain; 5, 31 -- format=flowed; 5, 31 -- charset="iso-8859-9"; 5, 31 -- reply-type=response 5, 31 -- Content-Transfer-Encoding: 7bit 5, 31 -- X-Priority: 3 5, 31 -- X-MSMail-Priority: Normal 5, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 5, 31 -- Disposition-Notification-To: "Nejat Yilmaz" {nyilmaz-at-mersin.edu.tr} 5, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 ==============================End of - Headers==============================
I have a request for information on what if any applications the SEM may have with paper products such as currency, lottery tickets, suspected fraud and counterfeit documents. The basis for the inquiry is whether a law enforcement group might justify obtaining an SEM to assist in investigation of suspected tampered documents and lottery tickets.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 4, 29 -- From dsherman-at-purdue.edu Fri Nov 14 15:17:26 2008 4, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAELHPE3021359 4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 15:17:26 -0600 4, 29 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 4, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id mAELHPaW021853 4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 16:17:25 -0500 4, 29 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 4, 29 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id mAELHPBV031942 4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 16:17:25 -0500 4, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 4, 29 -- Fri, 14 Nov 2008 16:17:23 -0500 4, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 4, 29 -- Fri, 14 Nov 2008 21:17:18 +0000 4, 29 -- User-Agent: Microsoft-Entourage/12.14.0.081024 4, 29 -- Date: Fri, 14 Nov 2008 16:17:17 -0500 4, 29 -- Subject: SEM and paper 4, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 4, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 4, 29 -- Message-ID: {C543550D.36357%dsherman-at-exchange.purdue.edu} 4, 29 -- Thread-Topic: SEM and paper 4, 29 -- Thread-Index: AclGnl8JAEYkmtdDSRWnFJ3glRyEAg== 4, 29 -- Mime-version: 1.0 4, 29 -- Content-type: text/plain; 4, 29 -- charset="US-ASCII" 4, 29 -- Content-transfer-encoding: 7bit 4, 29 -- X-OriginalArrivalTime: 14 Nov 2008 21:17:23.0451 (UTC) FILETIME=[62E1C4B0:01C9469E] 4, 29 -- X-PMX-Version: 5.4.0.320885 4, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: parmiterd-at-mail.nih.gov Name: David Parmiter
Organization: Nanotechnology Characterization Lab
Title-Subject: [Filtered] Position Opening - Senior Scientist
Question: SAIC-Frederick, Inc., a subsidiary of SAIC, develops and applies advanced technologies to meet the most urgent and challenging research and development needs of the National Cancer Institute, other government agencies, and the nation. We are the prime contractor for the National Cancer Institute at Frederick, one of 38 Federally Funded Research and Development Centers and the only such national laboratory devoted exclusively to biomedical research.
We are currently recruiting for a Senior Scientist (128940) at SAIC-Frederick. This scientist will manage the staff and operations of the electron microscopy (EM) laboratory at SAIC-Frederick. Specific duties include (i) managerial oversight of the EM laboratoryís core services (ii) interface with intra- and extramural investigators to provide EM characterization support (iii) methods development and research using techniques such as energy dispersive x-ray spectroscopy (EDS), cryogenics, focused ion beam ablation, 3D tomography on biological samples and (iv) interpretation of results and preparation of written reports. He/she will also work with an interdisciplinary team of scientists to characterize nanomaterials intended for cancer therapeutics and diagnostics.
Possession of a doctoral degree from an accredited college/ university in a field related to Chemistry, Biology, Material Science, Engineering or Physics. Foreign educated candidates who have completed part or all of their education outside of the United States must have their foreign education evaluated by an SAIC-approved accrediting organization to assure that it has met the equivalency of the qualifications of degree work in the United States. In addition to the educational requirements, a minimum of five years of related experience. A minimum of three years using electron microscopy (TEM, SEM) on biological tissues. Experience with energy dispersive x-ray spectroscopy (EDS), cryogenics, focused ion beam ablation, and 3D tomography. This position is subject to obtaining a Public Trust Clearance.
Excellent compensation package accompanies our position. For immediate consideration, please apply online at our website: www.saic.com for position #128940. SAIC is an equal opportunity employer and values cultural diversity in the workplace.
Laura Weddle (Contractor) Senior Employment Specialist SAIC-Frederick, Inc. National Cancer Institute at Frederick Post Office Box B Frederick, MD 21702 weddlel-at-mail.nih.gov
Debbie; The first SEM ever sold was the KCA Smith SEM sold to the Canadian Wood Pulp and Paper Institute, back around 1956. If that is not an endorsement of SEM's for paper analysis, I don't know what is.
John Mardinly, Numonyx
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Friday, November 14, 2008 1:25 PM To: MARDINLY, A
Hi all,
I have a request for information on what if any applications the SEM may have with paper products such as currency, lottery tickets, suspected fraud and counterfeit documents. The basis for the inquiry is whether a law enforcement group might justify obtaining an SEM to assist in investigation of suspected tampered documents and lottery tickets.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 4, 29 -- From dsherman-at-purdue.edu Fri Nov 14 15:17:26 2008 4, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAELHPE3021359 4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 15:17:26 -0600 4, 29 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 4, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id mAELHPaW021853 4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 16:17:25 -0500 4, 29 -- Received: from 1061exfe01a.itap.purdue.edu (1061exfe01a.itap.purdue.edu [128.210.1.8]) 4, 29 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id mAELHPBV031942 4, 29 -- for {microscopy-at-microscopy.com} ; Fri, 14 Nov 2008 16:17:25 -0500 4, 29 -- Received: from exch04.purdue.lcl ([172.21.6.23]) by 1061exfe01a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 4, 29 -- Fri, 14 Nov 2008 16:17:23 -0500 4, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 4, 29 -- Fri, 14 Nov 2008 21:17:18 +0000 4, 29 -- User-Agent: Microsoft-Entourage/12.14.0.081024 4, 29 -- Date: Fri, 14 Nov 2008 16:17:17 -0500 4, 29 -- Subject: SEM and paper 4, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 4, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 4, 29 -- Message-ID: {C543550D.36357%dsherman-at-exchange.purdue.edu} 4, 29 -- Thread-Topic: SEM and paper 4, 29 -- Thread-Index: AclGnl8JAEYkmtdDSRWnFJ3glRyEAg== 4, 29 -- Mime-version: 1.0 4, 29 -- Content-type: text/plain; 4, 29 -- charset="US-ASCII" 4, 29 -- Content-transfer-encoding: 7bit 4, 29 -- X-OriginalArrivalTime: 14 Nov 2008 21:17:23.0451 (UTC) FILETIME=[62E1C4B0:01C9469E] 4, 29 -- X-PMX-Version: 5.4.0.320885 4, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
==============================Original Headers============================== 13, 29 -- From A.MARDINLY-at-numonyx.com Fri Nov 14 15:37:38 2008 13, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAELbcZ8015240 13, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 14 Nov 2008 15:37:38 -0600 13, 29 -- Received: from exdresfenmx02.numonyx.local (unknown [10.96.252.23]) 13, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id F23044143B0; 13, 29 -- Fri, 14 Nov 2008 16:18:06 -0500 (EST) 13, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx02.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 13, 29 -- Fri, 14 Nov 2008 16:37:37 -0500 13, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 29 -- Content-class: urn:content-classes:message 13, 29 -- MIME-Version: 1.0 13, 29 -- Content-Type: text/plain; 13, 29 -- charset="us-ascii" 13, 29 -- Subject: RE: [Microscopy] SEM and paper 13, 29 -- Date: Fri, 14 Nov 2008 16:37:36 -0500 13, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9AD978B-at-EXDRESBENMX012.numonyx.local} 13, 29 -- In-Reply-To: {200811142125.mAELPRmp000723-at-ns.microscopy.com} 13, 29 -- X-MS-Has-Attach: 13, 29 -- X-MS-TNEF-Correlator: 13, 29 -- Thread-Topic: [Microscopy] SEM and paper 13, 29 -- Thread-Index: AclGn5dGqIyinzh/TOe4aekN/U2wjQAAMEEA 13, 29 -- References: {200811142125.mAELPRmp000723-at-ns.microscopy.com} 13, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 13, 29 -- To: {dsherman-at-purdue.edu} 13, 29 -- Cc: {Microscopy-at-Microscopy.com} 13, 29 -- X-OriginalArrivalTime: 14 Nov 2008 21:37:37.0323 (UTC) FILETIME=[3667EFB0:01C946A1] 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAELbcZ8015240 ==============================End of - Headers==============================
Debby Sherman asked whether a law enforcement group might justify obtaining an SEM to assist in investigation of suspected tampered documents and lottery tickets, based on SEM applications in the investigation of paper products such as currency, lottery tickets, suspected fraud and counterfeit documents.
REPLY:
A cardinal principle of comparative materials analysis applies here. One looks at the microstructure of a manufactured product to see what it is (for comparison with a designed or reference structure) and to see what the structure indicates about the process that formed the structure. In the case of the paper products mentioned, one should look at the type of fibers present, including special fibers with microscopic labels, the type and composition of the ink and other coatings, and the way the pattern is printed.
I'm not very familiar with the analysis of banknotes, but I do have a lot of experience in analyzing (with AFM) the structure of holographic decorations, some of which might be used as security or authenticity labels. It turns out that there are a variety of creating these decorations and a knowledge of the different processes and their hallmarks could well be useful in a forensic or intellectual property investigation.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
----- Original Message ----- From: dsherman-at-purdue.edu To: donc-at-asmicro.com Sent: Friday, November 14, 2008 4:21 PM Subject: [a] [Microscopy] SEM and paper
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Hi all,
I have a request for information on what if any applications the SEM may have with paper products such as currency, lottery tickets, suspected fraud and counterfeit documents. The basis for the inquiry is whether a law enforcement group might justify obtaining an SEM to assist in investigation of suspected tampered documents and lottery tickets.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 19, 25 -- From donc-at-asmicro.com Sat Nov 15 12:04:51 2008 19, 25 -- Received: from smtp106.sbc.mail.re2.yahoo.com (smtp106.sbc.mail.re2.yahoo.com [68.142.229.99]) 19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAFI4osj025733 19, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Nov 2008 12:04:50 -0600 19, 25 -- Received: (qmail 26713 invoked from network); 15 Nov 2008 18:04:50 -0000 19, 25 -- Received: from unknown (HELO asm15) (donc-at-68.51.122.238 with login) 19, 25 -- by smtp106.sbc.mail.re2.yahoo.com with SMTP; 15 Nov 2008 18:04:50 -0000 19, 25 -- X-YMail-OSG: LCLcRvIVM1n9Ckr90tOcq46Z_68RZGT8nGWvVg2Z9yr2FzpxlxpxCL7IeLXHenIf_mnebGdwNVGbm6Jg8SD.23545.XdoLWaRAuyxCwuJNkqwJliPf7W7Y25EB0bBRJ871lCmC38doY8rGHP.kdwFhyK6b.6MLCaL27qrDaBeCev5S1EB5G_oCwk62c- 19, 25 -- X-Yahoo-Newman-Property: ymail-3 19, 25 -- Message-ID: {E079C7488FA24901ADEF300A72FA0845-at-asm15} 19, 25 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 19, 25 -- To: {dsherman-at-purdue.edu} , "Microscopy List" {microscopy-at-microscopy.com} 19, 25 -- References: {200811142121.mAELL5Hj025905-at-ns.microscopy.com} 19, 25 -- Subject: Re: [a] [Microscopy] SEM and paper; holographic decorations 19, 25 -- Date: Sat, 15 Nov 2008 12:28:53 -0500 19, 25 -- MIME-Version: 1.0 19, 25 -- Content-Type: text/plain; 19, 25 -- format=flowed; 19, 25 -- charset="iso-8859-1"; 19, 25 -- reply-type=original 19, 25 -- Content-Transfer-Encoding: 7bit 19, 25 -- X-Priority: 3 19, 25 -- X-MSMail-Priority: Normal 19, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 19, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Here is a question I am almost embarrassed to ask. I am going to present an introductory TEM class, including tissue prep and sectioning. I need help finding fresh animal tissue for the students to fix and embed.
Actually, this may be more about the place I am at than anything. This is a community college, no big research labs or animal facility on campus. The campus policy regarding using animals in laboratory exercises is not totally clear to me at this time. I have a feeling that they are not going to be too keen on me whacking a mouse for its guts so the students can section liver etc. When I have mentioned my idea to some here, they look at me like I must be crazy. Some have suggested maybe I could go to the grocery store and pick up some samples. I am skeptical.
Is this a problem for anyone else? I could use some good ideas.
Jon
==============================Original Headers============================== 7, 36 -- From jkrupp-at-deltacollege.edu Sun Nov 16 20:53:01 2008 7, 36 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAH2r1J7029933 7, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Nov 2008 20:53:01 -0600 7, 36 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 7, 36 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 7, 36 -- with ESMTP id 43572943 for microscopy-at-microscopy.com; Sun, 16 Nov 2008 18:53:00 -0800 7, 36 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 7, 36 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 7, 36 -- ESMTP id KAGI0400.2XM for {microscopy-at-microscopy.com} ; Sun, 16 7, 36 -- Nov 2008 18:38:28 -0800 7, 36 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 403528F74BE4 7, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Nov 2008 18:53:00 -0800 (PST) 7, 36 -- X-Virus-Scanned: amavisd-new at 7, 36 -- X-Spam-Flag: NO 7, 36 -- X-Spam-Score: -2.499 7, 36 -- X-Spam-Level: 7, 36 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 7, 36 -- BAYES_00=-2.599, RDNS_NONE=0.1] 7, 36 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 7, 36 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 36 -- with ESMTP id eqD98WFb1U8c for {microscopy-at-microscopy.com} ; 7, 36 -- Sun, 16 Nov 2008 18:52:59 -0800 (PST) 7, 36 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) 7, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id DF2738F74BE3 7, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Nov 2008 18:52:59 -0800 (PST) 7, 36 -- Message-Id: {9DB087AC-810B-4455-80E8-7E74F1C7AF9D-at-deltacollege.edu} 7, 36 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 7, 36 -- To: microscopy-at-microscopy.com 7, 36 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 36 -- Content-Transfer-Encoding: 7bit 7, 36 -- Mime-Version: 1.0 (Apple Message framework v929.2) 7, 36 -- Subject: Animal tissues for students 7, 36 -- Date: Sun, 16 Nov 2008 18:52:59 -0800 7, 36 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
In our lab we a have a JEOL JSM 35-CF and we a have trouble with the CRT, half of the sreen is illuminated (Accelerating voltage is OFF and also the SE detector). Please see the image on the link, http://www.kaker.com/test/sem.jpg. Any idea, where is the error.
Best regards,
Henrik
Dr. Henrik Kaker SEM-EDS Lab Metal Ravne d.o.o. Koroska cesta 14 2390 Ravne Slovenia
==============================Original Headers============================== 6, 26 -- From Henrik.Kaker-at-guest.arnes.si Mon Nov 17 05:34:31 2008 6, 26 -- Received: from avs2.arnes.si (avs2.arnes.si [193.2.1.75]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAHBYUP0021516 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 17 Nov 2008 05:34:31 -0600 6, 26 -- Received: from localhost (unknown [193.2.1.75]) 6, 26 -- by avs2.arnes.si (Postfix) with ESMTP id 435DA4B80B2 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 17 Nov 2008 11:34:30 +0000 (UTC) 6, 26 -- X-Virus-Scanned: amavisd-new at arnes.si 6, 26 -- Received: from avs2.arnes.si ([193.2.1.75]) 6, 26 -- by localhost (avs2.arnes.si [193.2.1.75]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id WxJEfXxT5dZw for {microscopy-at-microscopy.com} ; 6, 26 -- Mon, 17 Nov 2008 12:34:30 +0100 (CET) 6, 26 -- Received: from [172.16.2.2] (unknown [193.189.163.74]) 6, 26 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 26 -- (No client certificate requested) 6, 26 -- by avs2.arnes.si (Postfix) with ESMTPS id 2B8834B80AE 6, 26 -- for {microscopy-at-microscopy.com} ; Mon, 17 Nov 2008 12:34:30 +0100 (CET) 6, 26 -- Message-ID: {4921FFFB.3050200-at-guest.arnes.si} 6, 26 -- Date: Tue, 18 Nov 2008 00:36:27 +0100 6, 26 -- From: Hernik Kaker {Henrik.Kaker-at-guest.arnes.si} 6, 26 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 6, 26 -- MIME-Version: 1.0 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Subject: Trouble with the JEOL JSM 35CF SEM 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
For an additional elementary reference document, see my web page - link just above the SEM images section. ..."Practical Primer" (a PDF file). www.albe24.com
Regards, Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
Hi, I was wondering what is good SEM reference book(s) to have on hand?
I would like one that talks about the analysis and measurements techniques. I would also like one that talks about the mechanical aspect of a SEM.
Thanks, Brian Chan MicroAssembly
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==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Mon Nov 17 07:21:27 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAHDLR53012093 2, 28 -- for {microscopy-at-microscopy.com} ; Mon, 17 Nov 2008 07:21:27 -0600 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.12198010; 2, 28 -- Mon, 17 Nov 2008 08:21:00 -0500 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.30]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Mon, 17 Nov 2008 08:20:59 -0500 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- Subject: RE: [Microscopy] SEM Reference Book 2, 28 -- Date: Mon, 17 Nov 2008 08:20:59 -0500 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7033F97AB-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200811140201.mAE21hOf007402-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] SEM Reference Book 2, 28 -- thread-index: AclF/P5dfmJ3mEN1QBu9R/tKwvjNNwCuZhQg 2, 28 -- References: {200811140201.mAE21hOf007402-at-ns.microscopy.com} 2, 28 -- To: {bchan-at-microassembly.com} , {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 17 Nov 2008 13:20:59.0993 (UTC) FILETIME=[550D7490:01C948B7] 2, 28 -- From: "White, N.W. (Woody)" {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="us-ascii" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAHDLR53012093 ==============================End of - Headers==============================
We use seedlings and young leaves (cotyledons and new growth) for plant tissues -- radish, mostly, but anything easy to sprout is good, and house crickets for animal tissue. From the crickets, you get good muscle preps, gut, Malphigian tubules (they're cool), brain, ventral nerve cord, and other neat things. Insects generally are good for this, as they are abundant, free, and animal care committees don't care about them. Also, pour an agar plate or two, leave it open for bit, and you'll have nice fungal colonies to work with. Or have the students bring in the moldy "what was this?" from their refrigerator.
Phil
} Hi: } } Here is a question I am almost embarrassed to ask. I am going to } present an introductory TEM class, including tissue prep and } sectioning. I need help finding fresh animal tissue for the students } to fix and embed. } } Actually, this may be more about the place I am at than anything. This } is a community college, no big research labs or animal facility on } campus. The campus policy regarding using animals in laboratory } exercises is not totally clear to me at this time. I have a feeling } that they are not going to be too keen on me whacking a mouse for its } guts so the students can section liver etc. When I have mentioned my } idea to some here, they look at me like I must be crazy. Some have } suggested maybe I could go to the grocery store and pick up some } samples. I am skeptical. } } Is this a problem for anyone else? I could use some good ideas. } } Jon -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 26 -- From oshel1pe-at-cmich.edu Mon Nov 17 07:34:18 2008 4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAHDYIAW025617 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Nov 2008 07:34:18 -0600 4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mAHDYACS018722; 4, 26 -- Mon, 17 Nov 2008 08:34:15 -0500 4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 26 -- Mon, 17 Nov 2008 08:33:15 -0500 4, 26 -- Mime-Version: 1.0 4, 26 -- Message-Id: {f06240804c54721ef339e-at-[141.209.160.249]} 4, 26 -- In-Reply-To: {200811170257.mAH2vvHO003488-at-ns.microscopy.com} 4, 26 -- References: {200811170257.mAH2vvHO003488-at-ns.microscopy.com} 4, 26 -- Date: Mon, 17 Nov 2008 08:33:14 -0500 4, 26 -- To: jkrupp-at-deltacollege.edu 4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 26 -- Subject: Re: [Microscopy] Animal tissues for students 4, 26 -- Cc: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 26 -- X-OriginalArrivalTime: 17 Nov 2008 13:33:15.0812 (UTC) FILETIME=[0BA28640:01C948B9] 4, 26 -- X-Canit-CHI2: 0.00 4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 26 -- X-CanItPRO-Stream: default 4, 26 -- X-Canit-Stats-ID: 5386570 - 2e357fa66360 4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
----- Original Message ----- X-from Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} Date Mon, 17 Nov 2008 09:24:37 GMT To jkrupp-at-deltacollege.edu Subject Re: [Microscopy] Animal tissues for students
Jon
one alternative to mammalian tissue would be to use the earthworm, but this would depend on whether it was the technique that you were interested in rather than the exact source of the tissue.
They can be both useful and available for light microscopy, although I don't know what they would be like for electron microscopy.
If you did use Lumbricus, then you would need to consider problems such as a gut content of soil and grit (eg keep them out of soil for a couple of days).
Malcolm
Malcolm Haswell e.m. unit University of Sunderland UK
----- Original Message ----- X-from: jkrupp-at-deltacollege.edu
Pel Freeze in Arkansas sells fresh organs from most parts of the rabbit.
www.pel-freez.com
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Sunday, November 16, 2008 9:03 PM To: Tim Holmes
Hi:
Here is a question I am almost embarrassed to ask. I am going to present an introductory TEM class, including tissue prep and sectioning. I need help finding fresh animal tissue for the students to fix and embed.
Actually, this may be more about the place I am at than anything. This is a community college, no big research labs or animal facility on campus. The campus policy regarding using animals in laboratory exercises is not totally clear to me at this time. I have a feeling that they are not going to be too keen on me whacking a mouse for its guts so the students can section liver etc. When I have mentioned my idea to some here, they look at me like I must be crazy. Some have suggested maybe I could go to the grocery store and pick up some samples. I am skeptical.
Is this a problem for anyone else? I could use some good ideas.
Jon
==============================Original Headers============================== 7, 36 -- From jkrupp-at-deltacollege.edu Sun Nov 16 20:53:01 2008 7, 36 -- Received: from sjdccd.cc.ca.us (smtp.deltacollege.edu [207.62.178.236]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAH2r1J7029933 7, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Nov 2008 20:53:01 -0600 7, 36 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 7, 36 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 7, 36 -- with ESMTP id 43572943 for microscopy-at-microscopy.com; Sun, 16 Nov 2008 18:53:00 -0800 7, 36 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 7, 36 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 7, 36 -- ESMTP id KAGI0400.2XM for {microscopy-at-microscopy.com} ; Sun, 16 7, 36 -- Nov 2008 18:38:28 -0800 7, 36 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 403528F74BE4 7, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Nov 2008 18:53:00 -0800 (PST) 7, 36 -- X-Virus-Scanned: amavisd-new at 7, 36 -- X-Spam-Flag: NO 7, 36 -- X-Spam-Score: -2.499 7, 36 -- X-Spam-Level: 7, 36 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 7, 36 -- BAYES_00=-2.599, RDNS_NONE=0.1] 7, 36 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 7, 36 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 36 -- with ESMTP id eqD98WFb1U8c for {microscopy-at-microscopy.com} ; 7, 36 -- Sun, 16 Nov 2008 18:52:59 -0800 (PST) 7, 36 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) 7, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id DF2738F74BE3 7, 36 -- for {microscopy-at-microscopy.com} ; Sun, 16 Nov 2008 18:52:59 -0800 (PST) 7, 36 -- Message-Id: {9DB087AC-810B-4455-80E8-7E74F1C7AF9D-at-deltacollege.edu} 7, 36 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 7, 36 -- To: microscopy-at-microscopy.com 7, 36 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 36 -- Content-Transfer-Encoding: 7bit 7, 36 -- Mime-Version: 1.0 (Apple Message framework v929.2) 7, 36 -- Subject: Animal tissues for students 7, 36 -- Date: Sun, 16 Nov 2008 18:52:59 -0800 7, 36 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
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==============================Original Headers============================== 20, 20 -- From holmes-at-lickenbrock.com Tue Nov 18 10:10:59 2008 20, 20 -- Received: from dc-2.lickenbrock.com (mail.lickenbrock.com [199.217.240.195]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAIGAxaG003189 20, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Nov 2008 10:10:59 -0600 20, 20 -- Content-class: urn:content-classes:message 20, 20 -- MIME-Version: 1.0 20, 20 -- Content-Type: text/plain; 20, 20 -- charset="us-ascii" 20, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 20 -- Subject: FW: [Microscopy] Animal tissues for students 20, 20 -- Date: Tue, 18 Nov 2008 10:10:52 -0600 20, 20 -- Message-ID: {42DEA6D518D1534DABD35B9CE4D6809610EE10-at-dc-2.lickenbrock.com} 20, 20 -- X-MS-Has-Attach: 20, 20 -- X-MS-TNEF-Correlator: 20, 20 -- Thread-Topic: [Microscopy] Animal tissues for students 20, 20 -- thread-index: AclIYPjHt4ZVgMSBSLOPg47oVrlJNABNsIMw 20, 20 -- From: "Tim Holmes" {holmes-at-lickenbrock.com} 20, 20 -- To: {Microscopy-at-microscopy.com} 20, 20 -- Content-Transfer-Encoding: 8bit 20, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAIGAxaG003189 ==============================End of - Headers==============================
That looks like a problem with had with an old image analysis system vintage 1980. The scan voltage was plus and minus for the different halves of the screen. No voltage in X or Y left the beam at the center of the screen. The scan circuits were similar but used complementary parts. I think the transistors were one digit off from each other. Anyway, the left side failed so the beam would sit at the center of the screen until the scan passed it and then it would scan as usual to the right side of the screen. Instead of flying back to the left edge, it only flew back to the center, and then waited. If you can slow the sweep down, you should be able to perceive the pause with your eyes.
I undertook the repairs myself under consultation with the engineers back at the factory. They guided me to the right board but they may not have been entirely sure which transistor was at fault. They recommended I try cooling one at a time with a cold blast from a duster can. When I found the right one, the scan returned to full left and right sweeps until the transistor warmed up again. Since they were big, discrete components, I was able to replace just the one part and get back to work.
May your problem be as simple.
Warren S.
-----Original Message----- X-from: Henrik.Kaker-at-guest.arnes.si [mailto:Henrik.Kaker-at-guest.arnes.si] Sent: Monday, November 17, 2008 5:35 AM To: wesaia-at-iastate.edu
Hello Everybody,
I have a user here who needs to be able to distinguish between two different sizes of axons, those 50um and under and those 51um and over. I think she measures along the longest side because some are more oval or odd shaped than round.
The images of interest are on the bottom of the page with no description, they are .gif format.
Because these sections are stained with toluidine blue and saved as gray scale, Image Pro Plus is having a hard time distinguishing between the two sizes. It sees the image as one big gray image with no distinct internal features. We haven't tried ImageJ as yet.
I know the images are of poor quality, out of focus and bad resolution but this is what we have to work with. I'm guessing that
If you have any suggestions as to how to make this counting process easier please let us know your suggestions.
Reply to either klbulacan-at-yahoo.com or to me.
Thank you for any help you may send us.
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 16, 25 -- From vapatpxs-at-yahoo.com Tue Nov 18 17:50:23 2008 16, 25 -- Received: from n61.bullet.mail.sp1.yahoo.com (n61.bullet.mail.sp1.yahoo.com [98.136.44.37]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAINoM5k013939 16, 25 -- for {microscopy-at-microscopy.com} ; Tue, 18 Nov 2008 17:50:22 -0600 16, 25 -- Received: from [69.147.65.174] by n61.bullet.mail.sp1.yahoo.com with NNFMP; 18 Nov 2008 23:50:22 -0000 16, 25 -- Received: from [69.147.65.170] by t12.bullet.mail.sp1.yahoo.com with NNFMP; 18 Nov 2008 23:50:22 -0000 16, 25 -- Received: from [127.0.0.1] by omp505.mail.sp1.yahoo.com with NNFMP; 18 Nov 2008 23:50:22 -0000 16, 25 -- X-Yahoo-Newman-Property: ymail-3 16, 25 -- X-Yahoo-Newman-Id: 401717.13007.bm-at-omp505.mail.sp1.yahoo.com 16, 25 -- Received: (qmail 16118 invoked by uid 60001); 18 Nov 2008 23:50:22 -0000 16, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 25 -- s=s1024; d=yahoo.com; 16, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 16, 25 -- b=LS/1VTKdAj9+ataerX3H8WMtdwYQAMZU0DEv0oH0W8/bNk28QltpRXNwNqxGLJX70xMrJ6iXCYa1mCGIGjKtYmJi1u3P2Rl3CB4MBVzlrBtVdQqaVPFAKKW8/LpHxfzEYosYSggesaQfm7wTB61QQ8LFQNOgQfik7F9EB6EAF1Y=; 16, 25 -- X-YMail-OSG: y0xBmGYVM1nspwezjJvW2mlWGHhClOONNIZDttrnEH3iEzsJiK4TZPrbfV8Mnd8Wb2XzvenClLWq7eOom3QebDsEbgygxk8FNgFnrhBcJrjLlkExtxlUxHAxWnDWTGRyH8n9rGCo57JmPbALt7v_mF.VLjba 16, 25 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Tue, 18 Nov 2008 15:50:21 PST 16, 25 -- X-Mailer: YahooMailWebService/0.7.260.1 16, 25 -- Date: Tue, 18 Nov 2008 15:50:21 -0800 (PST) 16, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 16, 25 -- Reply-To: vapatpxs-at-yahoo.com 16, 25 -- Subject: Images of axons 16, 25 -- To: IMAGEJ-at-LIST.NIH.GOV, MSA BB {Microscopy-at-microscopy.com} 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; charset=us-ascii 16, 25 -- Message-ID: {212975.15049.qm-at-web46101.mail.sp1.yahoo.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jmcclin-at-uky.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jmcclin-at-uky.edu Name: Joel McClintock
Organization: U of Kentucky
Title-Subject: [Filtered] SEM EDX Light Element Amplification
Question: We recently had a representative for Parallax Research come in and show us the new LoMAX focused optic product.
The paper presented shows that the product amplifies the light element range of an EDX detector and allows us to speed up mapping of light elements and find trace light elements in samples where the detectors straight from the manufacturer fail to do so in many applications. The price for the unit and installation is pretty reasonable, does anyone have one of these yet and what have your results with the attachment proven for you?
The explanation for the increase was based on a diffraction principle, how does this work?
It does not seem feasible that such an attachment would increase the signal for light elements by as much as 10x if the x-rays for the light elements were already there for the EDX detector to detect to start with. What are the drawbacks of this product?"
If you convert the image to RGB, then threshold the image, (I used gimp, but Photoshop or Image J should work) so that all pixels below 67 are white and all pixels above a value of 68 are black, it should be much easier to count the axons automatically, although some hand separation will be required. The thresholded image also makes it obvious that this is a composite image that wasn't stitched together very well.
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company Shelbyville, TN 37160 Direct: +1 (931) 685-6635
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==============================Original Headers============================== 5, 34 -- From Robert.Zonis-at-Sanford.com Wed Nov 19 10:10:51 2008 5, 34 -- Received: from mail96.messagelabs.com (mail96.messagelabs.com [216.82.254.19]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAJGAmRd031275 5, 34 -- for {Microscopy-at-Microscopy.Com} ; Wed, 19 Nov 2008 10:10:50 -0600 5, 34 -- X-VirusChecked: Checked 5, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 5, 34 -- X-Msg-Ref: server-7.tower-96.messagelabs.com!1227111047!31740187!2 5, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 5, 34 -- X-Originating-IP: [198.176.16.26] 5, 34 -- Received: (qmail 24950 invoked from network); 19 Nov 2008 16:10:48 -0000 5, 34 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) (198.176.16.26) 5, 34 -- by server-7.tower-96.messagelabs.com with SMTP; 19 Nov 2008 16:10:48 -0000 5, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 5, 34 -- Wed, 19 Nov 2008 10:10:47 -0600 5, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 34 -- Content-class: urn:content-classes:message 5, 34 -- MIME-Version: 1.0 5, 34 -- Content-Type: text/plain; 5, 34 -- charset="us-ascii" 5, 34 -- Subject: RE: [Microscopy] Images of axons 5, 34 -- Date: Wed, 19 Nov 2008 10:08:35 -0600 5, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A94A3D976-at-naoaksasebe02.nr.ad.newellco.com} 5, 34 -- In-Reply-To: {200811182356.mAINu6f7023078-at-ns.microscopy.com} 5, 34 -- X-MS-Has-Attach: 5, 34 -- X-MS-TNEF-Correlator: 5, 34 -- Thread-Topic: [Microscopy] Images of axons 5, 34 -- Thread-Index: AclJ2TphqwmG52YESoOfzcyOV/I5EQAhn4hQ 5, 34 -- References: {200811182356.mAINu6f7023078-at-ns.microscopy.com} 5, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 5, 34 -- To: {vapatpxs-at-yahoo.com} 5, 34 -- Cc: {Microscopy-at-Microscopy.Com} 5, 34 -- X-OriginalArrivalTime: 19 Nov 2008 16:10:47.0256 (UTC) FILETIME=[61F5ED80:01C94A61] 5, 34 -- Content-Transfer-Encoding: 8bit 5, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAJGAmRd031275 ==============================End of - Headers==============================
A few years ago I used Image Pro Plus a lot [as Bio-Rad's LaserPix] but for the last 7 years I've been exclusively a MetaMorph user, with occasional outings to the more limited freebie ImageJ. I attach a pdf with a few thoughts on your analysis. Despite using MetaMorph v7.5, much of what I suggest should be possible with Image Pro - their email help-line should be able to help as well [for free I'd hope].
Once you get a counting regime working have a go at getting it into a macro or two for semi-automatic counting [do a series of macros if you have to stop and go completely manual at any point, after which run the 'next' macro, etc..]. The 'Manual counting' option [by MetaMorph] may well be the fastest if your images just aren't up to it for easy [quick] thresholding.
Hope it all makes sense in the pdf as I've shot it off fairly quickly. Like PhotoShop image editing, there's always many ways of doing things.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: vapatpxs-at-yahoo.com [mailto:vapatpxs-at-yahoo.com] Sent: 18 November 2008 23:57 To: kjmorris-at-well.ox.ac.uk
Hello Everybody,
I have a user here who needs to be able to distinguish between two different sizes of axons, those 50um and under and those 51um and over. I think she measures along the longest side because some are more oval or odd shaped than round.
The images of interest are on the bottom of the page with no description, they are .gif format.
Because these sections are stained with toluidine blue and saved as gray scale, Image Pro Plus is having a hard time distinguishing between the two sizes. It sees the image as one big gray image with no distinct internal features. We haven't tried ImageJ as yet.
I know the images are of poor quality, out of focus and bad resolution but this is what we have to work with. I'm guessing that
If you have any suggestions as to how to make this counting process easier please let us know your suggestions.
Reply to either klbulacan-at-yahoo.com or to me.
Thank you for any help you may send us.
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 16, 25 -- From vapatpxs-at-yahoo.com Tue Nov 18 17:50:23 2008 16, 25 -- Received: from n61.bullet.mail.sp1.yahoo.com (n61.bullet.mail.sp1.yahoo.com [98.136.44.37]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAINoM5k013939 16, 25 -- for {microscopy-at-microscopy.com} ; Tue, 18 Nov 2008 17:50:22 -0600 16, 25 -- Received: from [69.147.65.174] by n61.bullet.mail.sp1.yahoo.com with NNFMP; 18 Nov 2008 23:50:22 -0000 16, 25 -- Received: from [69.147.65.170] by t12.bullet.mail.sp1.yahoo.com with NNFMP; 18 Nov 2008 23:50:22 -0000 16, 25 -- Received: from [127.0.0.1] by omp505.mail.sp1.yahoo.com with NNFMP; 18 Nov 2008 23:50:22 -0000 16, 25 -- X-Yahoo-Newman-Property: ymail-3 16, 25 -- X-Yahoo-Newman-Id: 401717.13007.bm-at-omp505.mail.sp1.yahoo.com 16, 25 -- Received: (qmail 16118 invoked by uid 60001); 18 Nov 2008 23:50:22 -0000 16, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 25 -- s=s1024; d=yahoo.com; 16, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:C ontent-Type:Message-ID; 16, 25 -- b=LS/1VTKdAj9+ataerX3H8WMtdwYQAMZU0DEv0oH0W8/bNk28QltpRXNwNqxGLJX70xMrJ6iXCY a1mCGIGjKtYmJi1u3P2Rl3CB4MBVzlrBtVdQqaVPFAKKW8/LpHxfzEYosYSggesaQfm7wTB61QQ8 LFQNOgQfik7F9EB6EAF1Y=; 16, 25 -- X-YMail-OSG: y0xBmGYVM1nspwezjJvW2mlWGHhClOONNIZDttrnEH3iEzsJiK4TZPrbfV8Mnd8Wb2XzvenClLWq 7eOom3QebDsEbgygxk8FNgFnrhBcJrjLlkExtxlUxHAxWnDWTGRyH8n9rGCo57JmPbALt7v_mF.V Ljba 16, 25 -- Received: from [132.239.85.200] by web46101.mail.sp1.yahoo.com via HTTP; Tue, 18 Nov 2008 15:50:21 PST 16, 25 -- X-Mailer: YahooMailWebService/0.7.260.1 16, 25 -- Date: Tue, 18 Nov 2008 15:50:21 -0800 (PST) 16, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 16, 25 -- Reply-To: vapatpxs-at-yahoo.com 16, 25 -- Subject: Images of axons 16, 25 -- To: IMAGEJ-at-LIST.NIH.GOV, MSA BB {Microscopy-at-microscopy.com} 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; charset=us-ascii 16, 25 -- Message-ID: {212975.15049.qm-at-web46101.mail.sp1.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 33, 20 -- From kjmorris-at-well.ox.ac.uk Wed Nov 19 10:25:02 2008 33, 20 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 33, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJGP15B024481 33, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 19 Nov 2008 10:25:01 -0600 33, 20 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 33, 20 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 33, 20 -- id 1L2prQ-0001ai-UB 33, 20 -- for Microscopy-at-Microscopy.Com; Wed, 19 Nov 2008 16:25:00 +0000 33, 20 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 33, 20 -- To: {Microscopy-at-Microscopy.Com} 33, 20 -- Subject: FW: [Microscopy] Images of axons 33, 20 -- Date: Wed, 19 Nov 2008 16:25:01 -0000 33, 20 -- Message-ID: {FA28E7EC0FCF4A23A7DBDEC2961B480D-at-CytoWhizz} 33, 20 -- MIME-Version: 1.0 33, 20 -- Content-Type: text/plain; 33, 20 -- charset="us-ascii" 33, 20 -- Content-Transfer-Encoding: 7bit 33, 20 -- X-Mailer: Microsoft Office Outlook 11 33, 20 -- Thread-Index: AclJ2VWf+tiAD+YHQg6ueSfJ2D9fzgAbp1YgAAC4kiA= 33, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 ==============================End of - Headers==============================
No. The name Pel "FREEZE" is misleading. They have fresh unfrozen samples. They ship them overnight unfrozen. We receive fresh whole eyes prepared special so that the tissue does not come in contact with the ice and this keeps cells from freezing. If you ask them, they will add such things as "antibiotic" and "antimitotic" solutions that keep them fresh for a few days without ruining the tissue properties (in our case we need optically clear corneas and lenses). When you receive them they are 24 hours "old."
-----Original Message----- X-from: Jon Krupp [mailto:jkrupp-at-deltacollege.edu] Sent: Wednesday, November 19, 2008 10:44 AM To: Tim Holmes
Joel, and listers/listees:
The Parallax Research LoMAX that attaches to the EDS detectors from virtually any MFG utilizes a focused optic that is highly reflective and sets close to the sample, much like the optics used by Parallax, Thermo, and EDAX with their parallel beam WDS systems. (Parallax is the source for the optics used by all three with the exception of the hybrid that Thermo uses to get a larger energy range, which is still part Parallax)
This optic does not absorb or allow for the high percentage of losses in the light element range due to the fact that the optic is "tuned" to diffract or reflect the wavelength of the energy range of 100 to about 900 eV. Without the LoMAX the x-rays have to be directly going towards the detector window or they are lost, the lower the energy, the easier they are lost due to direction and/or absorbance.
I have been working with Parallax to get the product ready and insure that it fits on most all 10mm and 30mm SiLi and 10mm SDD detectors since March. The last revision was tested on September 26th at UES and Parallax now offers the LoMAX to attach to the majority of SEM / EDS combinations. The UES report and a pic of the LoMAX is on the JoeXray website.
We also tested the LoMAX on a SEM/EDS combination at Case Western Reserve University last week and tested for artifacts and quant results. Using a Cr-Ni standard the cr and ni did not change when the O from the oxidized surface was not in the setup, when it was the O went from 1.4% to 4.3% using standardless quant on the NORAN System Six, showing that the EDS MFG standardless would not be appropriate for light elements in the mix, standards would be necessary. However the heavier SQ was not affected when the O was not in, which is good. The LoMAX comes with a Parallax provided collimator to allow the user to go back to "normal state" while keeping the calibrated adapter / trap in place to prevent the need for re-calibration when swapping from collimator to the LoMAX optic
Feel free to visit the JoeXray website (www.joexray.net) to see more and you may also go to the Parallax Research website as well at www.parallaxray.com.
---- jmcclin-at-uky.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both jmcclin-at-uky.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: jmcclin-at-uky.edu } Name: Joel McClintock } } Organization: U of Kentucky } } Title-Subject: [Filtered] SEM EDX Light Element Amplification } } Question: We recently had a representative for Parallax Research come } in and show us the new LoMAX focused optic product. } } The paper presented shows that the product amplifies the light } element range of an EDX detector and allows us to speed up mapping of } light elements and find trace light elements in samples where the } detectors straight from the manufacturer fail to do so in many } applications. The price for the unit and installation is pretty } reasonable, does anyone have one of these yet and what have your } results with the attachment proven for you? } } The explanation for the increase was based on a diffraction } principle, how does this work? } } It does not seem feasible that such an attachment would increase the } signal for light elements by as much as 10x if the x-rays for the } light elements were already there for the EDX detector to detect to } start with. What are the drawbacks of this product?" } } } Thank you all. } } } } Joel McClintock } } EM Specialist } } U of Kentucky } } Lexington, Ky } } } } Login Host: 74.140.182.126 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 19, 12 -- From zaluzec-at-microscopy.com Tue Nov 18 20:09:08 2008 } 19, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 19, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJ2955p029816 } 19, 12 -- for {microscopy-at-microscopy.com} ; Tue, 18 Nov 2008 20:09:08 -0600 } 19, 12 -- Mime-Version: 1.0 } 19, 12 -- Message-Id: {p06240806c549259a2b32-at-[206.69.208.22]} } 19, 12 -- Date: Tue, 18 Nov 2008 20:09:05 -0600 } 19, 12 -- To: microscopy-at-microscopy.com } 19, 12 -- From: jmcclin-at-uky.edu (by way of MicroscopyListserver) } 19, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: SEM EDX Light Element } 19, 12 -- Amplification } 19, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
-- Joe Ullmer
==============================Original Headers============================== 16, 21 -- From joexray-at-cinci.rr.com Wed Nov 19 16:09:12 2008 16, 21 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJM9B3U015744 16, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 16:09:12 -0600 16, 21 -- Received: from hrndva-web14-z01 ([10.128.132.105]) 16, 21 -- by hrndva-smta01.mail.rr.com with ESMTP 16, 21 -- id {20081119220911.LFIG24887.hrndva-smta01.mail.rr.com-at-hrndva-web14-z01} ; 16, 21 -- Wed, 19 Nov 2008 22:09:11 +0000 16, 21 -- Message-ID: {20081119220911.ASRWT.73850.root-at-hrndva-web14-z01} 16, 21 -- Date: Wed, 19 Nov 2008 17:09:11 -0500 16, 21 -- From: {joexray-at-cinci.rr.com} 16, 21 -- To: jmcclin-at-uky.edu, "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 21 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: SEM EDX 16, 21 -- Light Element 16, 21 -- In-Reply-To: {200811190215.mAJ2FksP010474-at-ns.microscopy.com} 16, 21 -- MIME-Version: 1.0 16, 21 -- Content-Type: text/plain; charset=utf-8 16, 21 -- Content-Transfer-Encoding: 7bit 16, 21 -- X-Priority: 3 (Normal) 16, 21 -- Sensitivity: Normal 16, 21 -- X-Originating-IP: ==============================End of - Headers==============================
I need advice. A researcher approached me about processing for TEM some (rare) human skin samples have already been formalin fixed, processed and embedded in paraffin. They are looking for specific cells in the stratum granulosum. They thought they could see them by light microscopy.
Has anyone done this before and have a protocol they would share? I have taken samples fixed in formalin and processed them for TEM, but the results were far from optimum. Before I embark on a long, involved procedure to melt the paraffin, etc., is it worth the effort? (They cannot get more tissue).
Thanks in advance. Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
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==============================Original Headers============================== 8, 26 -- From MSHERWOOD-at-PARTNERS.ORG Wed Nov 19 16:18:16 2008 8, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJMIGIB029251 8, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 16:18:16 -0600 8, 26 -- X-IronPort-AV: E=Sophos;i="4.33,634,1220241600"; 8, 26 -- d="scan'208";a="42917646" 8, 26 -- Received: from phsxcon5.mgh.harvard.edu (HELO PHSXCON5.partners.org) ([132.183.130.38]) 8, 26 -- by phsmgmx11.partners.org with ESMTP; 19 Nov 2008 17:18:16 -0500 8, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Wed, 19 Nov 2008 17:18:16 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Subject: [Microscopy] TEM processing of tissue embedded in paraffin 8, 26 -- Date: Wed, 19 Nov 2008 17:18:15 -0500 8, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C9CC-at-PHSXMB30.partners.org} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: [Microscopy] TEM processing of tissue embedded in paraffin 8, 26 -- Thread-Index: AclKlLfTsAxZDqNSRLqCCYAslOTzJg== 8, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 19 Nov 2008 22:18:16.0501 (UTC) FILETIME=[B855B650:01C94A94] 8, 26 -- Content-Type: text/plain; charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAJMIGIB029251 ==============================End of - Headers==============================
Hi Peggy, We process paraffin embedded tissue more often than we would like. You are correct, the results are far from optimum, probably less so than if were left in formalin! Our pathologist can sometimes make a diagnosis from the images but I doubt if they would ever publish them. If that is all the tissue you have, it may be worth a shot to give it a try. We cut out the area of the tissue desired, keeping as little paraffin around the tissue as little as possible then put the tissue piece into xylene overnight to melt/dissolve the paraffin. We then rehydrate from 100% ETOH to buffer, fix for at least 1 hour in Karnovsky's (our routine EM fix) then process as we do our other clinical samples. Years ago I worked for a clinical EM lab where we freshly mixed Osmium tetroxide with toluene and used that for the secondary fix. We didn't rehydrate the tissue just went on to embedding. Sorry, I don't have that protocol but maybe someone else can help you with that one. Good luck. Skin is tough so maybe you can get what your researcher needs. Pat Kysar University of California, Davis Medical School, Pathology EM Lab
----- Original Message ----- X-from: {MSHERWOOD-at-PARTNERS.ORG} To: {pekysar-at-ucdavis.edu} Sent: Wednesday, November 19, 2008 2:23 PM
Dear Claire,
Wouldn't it be nice if there was some real choice and competition for the business of a researcher buying a new ultramicrotome?!.
Unfortunately, as far as I know, there is only Leica and RMC to choose from. (Wait too long, and you'll only have one choice ;) ). I have used both, Leica much more extensively. I have no doubt that Leica's cryo unit is superior, even though the one from RMC does have some interesting features. Performance of the respective microtomes without cryo is very close, IMO. With Leica, you'll get a much larger user base - as well as a really lousy service, unfortunately. The price ought to be less for the RMC product, but I don't know it. The price for Leica's cryo unit seems about right, but the unit only will fit their newest microtome.
I don't think there is a "dedicated cryo ultramicrotome" available these days. There used to be some obscure offering from MicroStar, but I don't see it advertised anymore.
Posting this to all list hoping to be wrong and corrected.
Good luck, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Hello, } } I would like to know: } a.) the price range of cryo-ultramicrotome and } b.) different companies that sell in the US } } I have a Leica Ultracut R model which is a workhorse for doing } routine room temp sectioning. A cryo-attachment unit is available } for $36k. } } c.) what are the advantages/disadvantages of an attached cryo unit } versus a dedicated cryo ultramicrotome? } } Thank you in advance, } } Claire } } } } } ==============================Original } Headers============================== } 8, 20 -- From lukeclaire-at-yahoo.com Wed Nov 19 10:20:22 2008 } 8, 20 -- Received: from web55302.mail.re4.yahoo.com } (web55302.mail.re4.yahoo.com [206.190.58.181]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id mAJGKMMc013375 } 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 10:20:22 } -0600 } 8, 20 -- Received: (qmail 12793 invoked by uid 60001); 19 Nov 2008 } 16:20:21 -0000 } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply- } To:Subject:To:MIME-Version:Content-Type:Message-ID; } 8, 20 -- b=WT5FKvNS/UvOuASaIx1d } +0hRqOzJ8hN2loiLIYLdCqnVXPZQnxd2DzEY9JNDATUiOtvSfbkG67vW } +ghmQuqKOfKSN7Q8IuTAHAdO9M4BHdsX1EABOBpjtZvdlwFqo3t } +FDdIeu7kz4XQBflB5AkPZXn1cz3dqsguRj0C71lyB5E=; } 8, 20 -- X-YMail-OSG: } MX0WnZkVM1mMmVgMqKZmZadg5ab_uWjSvC8LizP7qa5wZHST_Rx4AsJVNl_nyLE3RiEo2DdRKE9kwSh0goMf5yM3qWNlfsO6wd9Xp3Zi5mbZgIAFP3KT9XZINcnvHdr7frKinEolq1lbY3 } .Zj0IuKZyqb2yFzHzjA9qHR1U- } 8, 20 -- Received: from [128.249.119.233] by } web55302.mail.re4.yahoo.com via HTTP; Wed, 19 Nov 2008 08:20:21 PST } 8, 20 -- X-Mailer: YahooMailWebService/0.7.260.1 } 8, 20 -- Date: Wed, 19 Nov 2008 08:20:21 -0800 (PST) } 8, 20 -- From: claire haueter {lukeclaire-at-yahoo.com} } 8, 20 -- Reply-To: lukeclaire-at-yahoo.com } 8, 20 -- Subject: Cryo-ultramicrotome } 8, 20 -- To: Microscopy-at-microscopy.com } 8, 20 -- MIME-Version: 1.0 } 8, 20 -- Content-Type: text/plain; charset=us-ascii } 8, 20 -- Message-ID: {847622.11931.qm-at-web55302.mail.re4.yahoo.com} } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From vladislav_speransky-at-nih.gov Wed Nov 19 17:39:04 2008 8, 23 -- Received: from nihrelayxway.hub.nih.gov (nihrelayxway.hub.nih.gov [128.231.90.106]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJNd3JU025086 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 17:39:04 -0600 8, 23 -- X-IronPortListener: NIH_Relay 8, 23 -- X-SBRS: None 8, 23 -- X-IronPort-AV: E=Sophos;i="4.33,635,1220241600"; 8, 23 -- d="scan'208";a="70402441" 8, 23 -- Received: from helix.nih.gov ([128.231.2.3]) 8, 23 -- by nihrelayxway.hub.nih.gov with ESMTP; 19 Nov 2008 18:39:03 -0500 8, 23 -- Received: from db447.niaid.nih.gov (db447.niaid.nih.gov [128.231.217.47]) 8, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id mAJNd1tU022407 8, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 18:39:03 -0500 8, 23 -- Message-Id: {ADA905BE-9588-4591-8B95-BC9921464017-at-nih.gov} 8, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- Mime-Version: 1.0 (Apple Message framework v929.2) 8, 23 -- Subject: Fwd: [Microscopy] Cryo-ultramicrotome 8, 23 -- Date: Wed, 19 Nov 2008 18:39:01 -0500 8, 23 -- References: {200811191621.mAJGLAcB015771-at-ns.microscopy.com} 8, 23 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I am trying to locate a diamond scriber that is housed in a modified objective lens. Basically, one locates an area of interest in the light microscope, switches the scriber/objective into position and rotates the objective to scribe a small circle around the area of interest. I believe Zeiss made such a device, but I am wondering if other manufacturers make one was well? -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
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I have rather limited experience with RMC, so I can only express my content with the Leica new microtome and cryounit I have been using for some 3 years.
The complaints about the service in the DC area are quite common. We use M.O.C. and are very happy about their service - timely, qualified and (I believe) competitively priced. They probably service the area from New England down to Philadelphia.
No interest in M.O.C., just a happy customer.
Michal
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Dear Claire,
Wouldn't it be nice if there was some real choice and competition for the business of a researcher buying a new ultramicrotome?!.
Unfortunately, as far as I know, there is only Leica and RMC to choose from. (Wait too long, and you'll only have one choice ;) ). I have used both, Leica much more extensively. I have no doubt that Leica's cryo unit is superior, even though the one from RMC does have some interesting features. Performance of the respective microtomes without cryo is very close, IMO. With Leica, you'll get a much larger user base - as well as a really lousy service, unfortunately. The price ought to be less for the RMC product, but I don't know it. The price for Leica's cryo unit seems about right, but the unit only will fit their newest microtome.
I don't think there is a "dedicated cryo ultramicrotome" available these days. There used to be some obscure offering from MicroStar, but I don't see it advertised anymore.
Posting this to all list hoping to be wrong and corrected.
Good luck, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Hello, } } I would like to know: } a.) the price range of cryo-ultramicrotome and } b.) different companies that sell in the US } } I have a Leica Ultracut R model which is a workhorse for doing } routine room temp sectioning. A cryo-attachment unit is available } for $36k. } } c.) what are the advantages/disadvantages of an attached cryo unit } versus a dedicated cryo ultramicrotome? } } Thank you in advance, } } Claire } } } } } ==============================Original } Headers============================== } 8, 20 -- From lukeclaire-at-yahoo.com Wed Nov 19 10:20:22 2008 } 8, 20 -- Received: from web55302.mail.re4.yahoo.com } (web55302.mail.re4.yahoo.com [206.190.58.181]) } 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id mAJGKMMc013375 } 8, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 10:20:22 } -0600 } 8, 20 -- Received: (qmail 12793 invoked by uid 60001); 19 Nov 2008 } 16:20:21 -0000 } 8, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 8, 20 -- s=s1024; d=yahoo.com; } 8, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply- } To:Subject:To:MIME-Version:Content-Type:Message-ID; } 8, 20 -- b=WT5FKvNS/UvOuASaIx1d } +0hRqOzJ8hN2loiLIYLdCqnVXPZQnxd2DzEY9JNDATUiOtvSfbkG67vW } +ghmQuqKOfKSN7Q8IuTAHAdO9M4BHdsX1EABOBpjtZvdlwFqo3t } +FDdIeu7kz4XQBflB5AkPZXn1cz3dqsguRj0C71lyB5E=; } 8, 20 -- X-YMail-OSG: } MX0WnZkVM1mMmVgMqKZmZadg5ab_uWjSvC8LizP7qa5wZHST_Rx4AsJVNl_nyLE3RiEo2DdRKE9kwSh0goMf5yM3qWNlfsO6wd9Xp3Zi5mbZgIAFP3KT9XZINcnvHdr7frKinEolq1lbY3
Before I did this, I would find out what in the cells they are specifically looking for. I often have to deparaffinize tissue for diagnostic TEM and one of the main problems that you are going to encounter is that a lot of your cellular membranes and small organelles are going to get washed away. You are then basing your intereptation more on experience, than on actual data. I think your researcher would be better served trying to find an immunohistochemcial solution before trying EM.
Good luck, Steve
Steven Lee Chief Technologist Electron Microscopy Laboratory Baylor University Medical Center 3500 Gaston Ave Dallas, TX 75246 6 214.820.3302 Ê 214.820.4110 2 stevenle-at-baylorhealth.edu
-----Original Message----- X-from: MSHERWOOD-at-PARTNERS.ORG [mailto:MSHERWOOD-at-PARTNERS.ORG] Sent: Wednesday, November 19, 2008 4:21 PM To: Lee, Steven
To all:
I need advice. A researcher approached me about processing for TEM some (rare) human skin samples have already been formalin fixed, processed and embedded in paraffin. They are looking for specific cells in the stratum granulosum. They thought they could see them by light microscopy.
Has anyone done this before and have a protocol they would share? I have taken samples fixed in formalin and processed them for TEM, but the results were far from optimum. Before I embark on a long, involved procedure to melt the paraffin, etc., is it worth the effort? (They cannot get more tissue).
Thanks in advance. Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information.
==============================Original Headers============================== 8, 26 -- From MSHERWOOD-at-PARTNERS.ORG Wed Nov 19 16:18:16 2008 8, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJMIGIB029251 8, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 16:18:16 -0600 8, 26 -- X-IronPort-AV: E=Sophos;i="4.33,634,1220241600"; 8, 26 -- d="scan'208";a="42917646" 8, 26 -- Received: from phsxcon5.mgh.harvard.edu (HELO PHSXCON5.partners.org) ([132.183.130.38]) 8, 26 -- by phsmgmx11.partners.org with ESMTP; 19 Nov 2008 17:18:16 -0500 8, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Wed, 19 Nov 2008 17:18:16 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Subject: [Microscopy] TEM processing of tissue embedded in paraffin 8, 26 -- Date: Wed, 19 Nov 2008 17:18:15 -0500 8, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C9CC-at-PHSXMB30.partners.org} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: [Microscopy] TEM processing of tissue embedded in paraffin 8, 26 -- Thread-Index: AclKlLfTsAxZDqNSRLqCCYAslOTzJg== 8, 26 -- From: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 19 Nov 2008 22:18:16.0501 (UTC) FILETIME=[B855B650:01C94A94] 8, 26 -- Content-Type: text/plain; charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAJMIGIB029251 ==============================End of - Headers==============================
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==============================Original Headers============================== 26, 32 -- From StevenLe-at-BaylorHealth.edu Thu Nov 20 07:18:53 2008 26, 32 -- Received: from bhdappagnt01.baylorhealth.edu ([4.22.141.163]) 26, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAKDImYS023309 26, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 07:18:53 -0600 26, 32 -- Received: from BHDAEXIMS01.bhcs.pvt ([10.5.12.120]) 26, 32 -- by bhdappagnt01.baylorhealth.edu (8.14.1/8.14.1) with ESMTP id mAKDIhwd019967 26, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 07:18:43 -0600 26, 32 -- X-TM-IMSS-Message-ID: {1261daca00029b92-at-bhcs.pvt} 26, 32 -- Received: from BHDAEXHT01.bhcs.pvt ([10.5.12.193]) by bhcs.pvt ([10.5.12.120]) with ESMTP (TREND IMSS SMTP Service 7.0; TLS: TLSv1/SSLv3,128bits,RC4-MD5) id 1261daca00029b92 for {StevenLe-at-BaylorHealth.edu} ; Thu, 20 Nov 2008 07:18:07 -0600 26, 32 -- Received: from BHDAEXVM32.bhcs.pvt ([10.5.3.122]) by BHDAEXHT01.bhcs.pvt 26, 32 -- ([10.5.12.193]) with mapi; Thu, 20 Nov 2008 07:18:07 -0600 26, 32 -- From: "Lee, Steven" {StevenLe-at-BaylorHealth.edu} 26, 32 -- To: "'MSHERWOOD-at-PARTNERS.ORG'" {MSHERWOOD-at-PARTNERS.ORG} 26, 32 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 26, 32 -- Date: Thu, 20 Nov 2008 07:18:05 -0600 26, 32 -- Subject: RE: [Microscopy] TEM processing of tissue embedded in paraffin 26, 32 -- Thread-Topic: [Microscopy] TEM processing of tissue embedded in paraffin 26, 32 -- Thread-Index: AclKlR+J2yePIqcdQOGgkhroLH5APQAekWHw 26, 32 -- Message-ID: {492DD4D34D5DC5489E59D73B0506681C0CA89A8E07-at-BHDAEXVM32.bhcs.pvt} 26, 32 -- References: {200811192221.mAJML8FY004393-at-ns.microscopy.com} 26, 32 -- In-Reply-To: {200811192221.mAJML8FY004393-at-ns.microscopy.com} 26, 32 -- Accept-Language: en-US 26, 32 -- Content-Language: en-US 26, 32 -- X-MS-Has-Attach: 26, 32 -- X-MS-TNEF-Correlator: 26, 32 -- acceptlanguage: en-US 26, 32 -- Content-Type: text/plain; charset="iso-8859-1" 26, 32 -- MIME-Version: 1.0 26, 32 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-11-20_10:2008-11-19,2008-11-20,2008-11-20 signatures=0 26, 32 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=5.0.0-0810130000 definitions=main-0811200027 26, 32 -- Content-Transfer-Encoding: 8bit 26, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAKDImYS023309 ==============================End of - Headers==============================
Yes the ultrastructure will be terrible, but may be adequate for an answer.
I dissolve OsO4 crystals in toluene (1%), cut out the tissue from the paraffin block, mince into 1mm pieces and place pieces in the osmium/toluene overnight. Rinse with acetone three times to remove osmium and then infiltrate as you normally would with your standard epoxy resin.
The paraffin processing will remove most membranes, all lipids, shrink the tissue about 20%, and numerous other artifacts will appear. Exactly what type of cells are they looking for? Cytoplasmic filaments will be stable. Most protein inclusions will stay put but the ultrastructure may be hard to recognize. Cell junctions will still be present.
The may thing to let the person know is that the results may not yield any valid answer and get them to think of EM to begin with.
Best of luck,
Ed
Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu -----Original Message----- X-from: MSHERWOOD-at-PARTNERS.ORG [mailto:MSHERWOOD-at-PARTNERS.ORG] Sent: Wednesday, November 19, 2008 5:21 PM To: Calomeni, Edward
To all:
I need advice. A researcher approached me about processing for TEM some (rare) human skin samples have already been formalin fixed, processed and embedded in paraffin. They are looking for specific cells in the stratum granulosum. They thought they could see them by light microscopy.
Has anyone done this before and have a protocol they would share? I have taken samples fixed in formalin and processed them for TEM, but the results were far from optimum. Before I embark on a long, involved procedure to melt the paraffin, etc., is it worth the effort? (They cannot get more tissue).
Thanks in advance. Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
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==============================Original Headers============================== 8, 26 -- From MSHERWOOD-at-PARTNERS.ORG Wed Nov 19 16:18:16 2008 8, 26 -- Received: from phsmgmx11.partners.org (phsmgmx11.partners.org [155.52.251.65]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAJMIGIB029251 8, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 16:18:16 -0600 8, 26 -- X-IronPort-AV: E=Sophos;i="4.33,634,1220241600"; 8, 26 -- d="scan'208";a="42917646" 8, 26 -- Received: from phsxcon5.mgh.harvard.edu (HELO PHSXCON5.partners.org) ([132.183.130.38]) 8, 26 -- by phsmgmx11.partners.org with ESMTP; 19 Nov 2008 17:18:16 -0500 8, 26 -- Received: from PHSXMB30.partners.org ([170.223.98.113]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.3959); 8, 26 -- Wed, 19 Nov 2008 17:18:16 -0500 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Subject: [Microscopy] TEM processing of tissue embedded in paraffin 8, 26 -- Date: Wed, 19 Nov 2008 17:18:15 -0500 8, 26 -- Message-ID: {073AE2BEA1C2BA4A8837AB6C4B943D9701A7C9CC-at-PHSXMB30.partners.org} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: [Microscopy] TEM processing of tissue embedded in paraffin 8, 26 -- Thread-Index: AclKlLfTsAxZDqNSRLqCCYAslOTzJg== 8, 26 -- X-from: "Sherwood, Margaret " {MSHERWOOD-at-PARTNERS.ORG} 8, 26 -- To: {Microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 19 Nov 2008 22:18:16.0501 (UTC) FILETIME=[B855B650:01C94A94] 8, 26 -- Content-Type: text/plain; charset="us-ascii" 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAJMIGIB029251 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 36 -- From Edward.Calomeni-at-osumc.edu Thu Nov 20 07:31:35 2008 23, 36 -- Received: from TWAPP-VP01.OSUMC.EDU (pluto.osumc.edu [140.254.120.27]) 23, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAKDVYAI004535 23, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 07:31:34 -0600 23, 36 -- Received: from [140.254.138.19] by TWAPP-VP01.OSUMC.EDU with ESMTP ( 23, 36 -- OSUMC **** SMTP Relay ****); Thu, 20 Nov 2008 08:31:27 -0500 23, 36 -- X-Server-Uuid: B9562FAF-5C56-437C-B754-33221F224476 23, 36 -- Received: from localhost (unknown [127.0.0.1]) by pfeg02.osumc.edu ( 23, 36 -- Postfix) with ESMTP id 7F35C3EB14 for {Microscopy-at-microscopy.com} ; Thu, 23, 36 -- 20 Nov 2008 13:31:27 +0000 (UTC) 23, 36 -- Received: from pfeg02.osumc.edu ([127.0.0.1]) by localhost (pfeg02 23, 36 -- [127.0.0.1]) (amavisd-new, port 10024) with LMTP id 14368-01-22 for 23, 36 -- {Microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 08:31:27 -0500 (EST) 23, 36 -- Received: from msxc01.OSUMC.EDU (msxc01.osumc.edu [10.127.29.33]) by 23, 36 -- pfeg02.osumc.edu (Postfix) with ESMTP id 5BBF23CBBE for 23, 36 -- {Microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 08:31:27 -0500 (EST) 23, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 36 -- Content-class: urn:content-classes:message 23, 36 -- MIME-Version: 1.0 23, 36 -- Subject: RE: [Microscopy] TEM processing of tissue embedded in paraffin 23, 36 -- Date: Thu, 20 Nov 2008 08:31:27 -0500 23, 36 -- Message-ID: {7A541592F9A53A4E86E7A3D5C4C7F68C024940C4-at-msxc01.OSUMC.EDU} 23, 36 -- In-Reply-To: {200811192220.mAJMKUPa002837-at-ns.microscopy.com} 23, 36 -- X-MS-Has-Attach: 23, 36 -- X-MS-TNEF-Correlator: 23, 36 -- Thread-Topic: [Microscopy] TEM processing of tissue embedded in paraffin 23, 36 -- thread-index: AclKlQnLwssEa4Z1RYSIUzK0i+WWPgAfd6Gg 23, 36 -- References: {200811192220.mAJMKUPa002837-at-ns.microscopy.com} 23, 36 -- From: "Calomeni, Edward " {Edward.Calomeni-at-osumc.edu} 23, 36 -- To: Microscopy-at-microscopy.com 23, 36 -- X-Virus-Scanned: amavisd-new at osumc.edu 23, 36 -- X-WSS-ID: 653BB9251T8970259-01-01 23, 36 -- Content-Type: text/plain; 23, 36 -- charset=us-ascii 23, 36 -- Content-Transfer-Encoding: 8bit 23, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAKDVYAI004535 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scott.streiker-at-udri.udayton.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scott.streiker-at-udri.udayton.edu Name: Scott Streiker
Organization: University of Dayton Research Institute
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both E.Miller-at-curtin.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: E.Miller-at-curtin.edu.au Name: Elaine Miller
Organization: Curtin University of Technology, Perth, W Australia
Title-Subject: [Filtered] Coating for FIBSEM
Question: We recently have acquired a Zeiss Neon FIBSEM with FESEM capabilities. I was wondering if anyone could give me any advice on a suitable coating unit. We have to replace our old gold sputter coater so I was hoping to buy something that could do both high resolution (platinum?) coatings and regular gold coats for SEM. I have read about Osmium plasma coating but that is beyond our means at the moment and I am a bit worried about the OHS issues. Is platinum the way to go? Does anyone have any good/bad experiences to point me in any particular direction before I commit to a purchase?
We use several of these in the lab to mark fields of view before moving preps from 'scope to 'scope. After a brief survey of the ones we have, I can tell you that they are manufactured both by Zeiss (under the Winkel imprint in our case) and Leica (as Leitz Wetzlar). Sadly, I don't think they are produced any longer, so you're relegated to ebay to find used ones -- and they're pretty rare there, as well. The Leitz ones are pretty simple instruments, and our machine shop has repaired them in the past. If their measurements are complete, I could send them on to you -- assuming you could source the diamond chip that's in the tip of the scribe, a reasonable machine shop should be able to build you something similar, though you may have to lend them one of your objectives so that they could measure thread pitch and diameter. Let me know if you'd like the additional info, and best of luck finding some of these excellent old scribes.
Shawn
Shawn Galdeen, Ph.D. Post-Doctoral Associate Sluder Lab University of Massachusetts Medical School 508.856.8653
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I am trying to locate a diamond scriber that is housed in a modified objective lens. Basically, one locates an area of interest in the light microscope, switches the scriber/objective into position and rotates the objective to scribe a small circle around the area of interest. I believe Zeiss made such a device, but I am wondering if other manufacturers make one was well? -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
We have recently purchased (but haven't had a chance to try yet) an "Object Marker" from Zeiss that can be screwed into one of the objective ports, and swung into place to put an ink marking on a coverslip on an area that you have predetermined with another objective. The directions say that the markings quickly become smear-resistant in air, but will dissolve slowly when covered with immersion oil. The product # is 1105-072 (can't remember what we paid for it, but I will look it up if anyone wants to know). Can't say how it works yet, but it is possibly an alternative to apparently no longer available diamond scribers.
Cheers
shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1760 Facsimile/Télécopieur: 613-759-1701 Rm 2068 K.W. Neatby Bldg 960 Carling Ave. Central Experimental Farm Ottawa, ON K1A 0C6 millers-at-agr.gc.ca
==============================Original Headers============================== 8, 21 -- From MILLERS-at-AGR.GC.CA Thu Nov 20 09:00:59 2008 8, 21 -- Received: from agrpazsmtp8.agr.gc.ca (agrpazsmtp8.agr.gc.ca [192.197.71.119]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAKF0xr1028281 8, 21 -- for {Microscopy-at-Microscopy.Com} ; Thu, 20 Nov 2008 09:00:59 -0600 8, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 21 -- Content-class: urn:content-classes:message 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain; 8, 21 -- charset="iso-8859-1" 8, 21 -- Subject: diamond scriber in objective 8, 21 -- Date: Thu, 20 Nov 2008 10:00:56 -0500 8, 21 -- Message-ID: {FD493497DF264A4E8DC71F1FFE49129DD576DD-at-ONOTTAXMS2.AGR.GC.CA} 8, 21 -- X-MS-Has-Attach: 8, 21 -- X-MS-TNEF-Correlator: 8, 21 -- Thread-Topic: diamond scriber in objective 8, 21 -- Thread-Index: AclLIMJeBM58TKcqQECV0m51mmifaQ== 8, 21 -- From: "Miller, Shea" {MILLERS-at-AGR.GC.CA} 8, 21 -- To: {Microscopy-at-Microscopy.Com} 8, 21 -- X-OriginalArrivalTime: 20 Nov 2008 15:00:57.0083 (UTC) FILETIME=[CAD604B0:01C94B20] 8, 21 -- Content-Transfer-Encoding: 8bit 8, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAKF0xr1028281 ==============================End of - Headers==============================
We still use Kodak 35mm film #5302 (fine grain positive) in our older TEM but have not been able to find any more stock at the usual supply houses in North America.
So, does
1. Anyone know of existing stock of Kodak #5302? 2. Anyone have unused film left in their lab? (expired OK) 3. Anyone suggest a substitute?
Thanks, Karen Rethoret Microscopy Facility York University Toronto, Canada 416-736-2100 x33289
==============================Original Headers============================== 6, 17 -- From rethoret-at-yorku.ca Thu Nov 20 09:37:28 2008 6, 17 -- Received: from tweety.ccs.yorku.ca (tweety.ccs.yorku.ca [130.63.236.216]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAKFbSfJ010076 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 20 Nov 2008 09:37:28 -0600 6, 17 -- Received: from localhost (emlab.biol.yorku.ca [130.63.242.104]) 6, 17 -- by tweety.ccs.yorku.ca (8.14.1/8.14.1/Debian-8ubuntu1) with ESMTP id mAKFbRg7011432 6, 17 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT) 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Thu, 20 Nov 2008 10:37:27 -0500 6, 17 -- Date: Thu, 20 Nov 2008 10:37:27 -0500 (Eastern Standard Time) 6, 17 -- From: Karen Rethoret {rethoret-at-yorku.ca} 6, 17 -- To: Microscopy-at-Microscopy.Com 6, 17 -- Subject: Kodak 5302 film 6, 17 -- Message-ID: {Pine.WNT.4.64.0811201032300.2408-at-rethoret1} 6, 17 -- Organization: York University 6, 17 -- X-X-Sender: rethoret-at-mypost.yorku.ca 6, 17 -- MIME-Version: 1.0 6, 17 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
It would seem that I spoke a bit soon concerning availability of diamond scribe objectives. Cheryl Rehfield from Meyer was kind enough to contact me and mention that Leica objective scribes are still available, and a quick search of the listserver archives shows that Zeiss ones were also available as of 2002 ( http://www.microscopy.com/cgi-bin/ReadPrintEmailHTML.pl?filename=0205.txt , search for text 'diamond marker'). Here's the info that I now know about each:
Zeiss diamond marker: part #462960, $1,385 (as of 2002).
Leica 'object marker with thread M25': part #11505059, $1,061 (per Cheryl)
As always, I have no commerical interest with Meyer or any other company, just wanted to share this information for those still looking.
Best wishes,
Shawn
Shawn Galdeen, Ph.D. Post-Doctoral Associate Sluder Lab University of Massachusetts Medical School 508.856.8653
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I am trying to locate a diamond scriber that is housed in a modified objective lens. Basically, one locates an area of interest in the light microscope, switches the scriber/objective into position and rotates the objective to scribe a small circle around the area of interest. I believe Zeiss made such a device, but I am wondering if other manufacturers make one was well? -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I received an email yesterday evening from a senior RMC executive pointing out that some of my comments in the earlier post could be interpreted in such a way that I have knowledge that RMC ultramicrotomes might not be available in the future.
I would like to make it very clear that this was just a light-hearted joke (see the wink) coming from someone freshly frustrated by Leica's CS and hinting at Leica's recent acquisition of BalTec. I sincerely apologize for any potential damage this might have caused to list members' perception of RMC.
I would also like to change one of the opinion statements in the post to: "I have no doubt that Leica's cryo unit is superior *for certain applications*. On the other hand, the cryo unit from RMC does have some interesting features, *and I personally know at least one very prominent and technologically advanced EM/Cell Biology lab in Europe who chose to get several RMC cryoultramicrotomes - because they have found them superior*". (Name available off-list.)
Finally: I have been using two Leica's Ultracuts with FCS cryo chamber on a regular basis since 1999 to cut sucrose-protected cryosections. I used an RMC cryo setup for several weeks (2-3 months total) in 1998-99 and later (2004?) went to a local mini cryo workshop held by RMC.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and not his employer. These opinions and experiences do not represent a consensus in the NIH scientific community.
==============================Original Headers============================== 7, 22 -- From vladislav_speransky-at-nih.gov Thu Nov 20 10:51:50 2008 7, 22 -- Received: from out1.smtp.messagingengine.com (out1.smtp.messagingengine.com [66.111.4.25]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAKGpodt011075 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 10:51:50 -0600 7, 22 -- Received: from compute2.internal (compute2.internal [10.202.2.42]) 7, 22 -- by out1.messagingengine.com (Postfix) with ESMTP id CB0F21C5649 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 11:51:49 -0500 (EST) 7, 22 -- Received: from heartbeat1.messagingengine.com ([10.202.2.160]) 7, 22 -- by compute2.internal (MEProxy); Thu, 20 Nov 2008 11:51:49 -0500 7, 22 -- X-Sasl-enc: id3lx8tpdZho+LYa9S7UfEZIDgcqqdGz0chZ0fJ8Q4Sm 1227199909 7, 22 -- Received: from [192.168.0.5] (unknown [71.178.189.167]) 7, 22 -- by mail.messagingengine.com (Postfix) with ESMTPA id 7DD3833C8 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 11:51:49 -0500 (EST) 7, 22 -- Message-Id: {C2A1A8A9-EFE6-4F49-BE45-B8394DF19AC0-at-nih.gov} 7, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 7, 22 -- Subject: [Microscopy] Cryo-ultramicrotome - CORRECTION 7, 22 -- Date: Thu, 20 Nov 2008 11:51:48 -0500 7, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I would like to thank everybody for trying to help with the awful axon image and how to count them. I found out that the person did not coverslip the sections, helped make the images look bad. Plus the stitching is awful, she didn't use on of my microscopes! ;)
She is trying to separate the axons by area size, so I was mistaken on that point.
Her problem is that with the uniform staining (everything is gray/blue) that the axons appear to touch each other when she thresholds the axons and she doesn't want to spend a lot of time manually separating the touching axons.
I think she's going to have to do a lot of steps manually, that stinks but that's science.
I'm going to help her to get better images and see what happens.
So, once again, thank you for all your assistance.
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
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I just want to thank all of the people who responded to my query. The consensus of many was that it is generally not a good idea to reproccess FFPE tissue for TEM. The paraffin processing destroys cellular membranes, many organelles, as well as the nuclear envelope and chromatin.
Many people did offer protocols, which I may try, depending on the desire of the investigator. And some people offered to send me images that they had taken of such processed tissue. One person said I should try and dissuade the investigator from pursuing this! I actually tell people to divide the specimen and fix in both formalin and Karnovskys.
I really value this ListServer. When it is used appropriately, it is a invaluable source of information and help.
Again many thanks to all who offered advice.
Have a wonderful Thanksgiving!
Peggy
Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood-at-partners.org
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Elaine: I have 2 FESEMs and use iridium for everything, including my FIB work. I will sometimes use evaporative carbon for FIB work, especially if I need to image using the ion beam extensively (carbon's low sputter yield means it will stay on the surface longer). If I need to do thickness measurements of thin layers, I put down a layer of carbon before I put down my metal deposition to make the cut. The carbon layer makes it easier to separate the surface metal from the metal I put down. All my sputter coaters are from Emitech and I'm happy with them.
E.Miller-at-curtin.edu.au wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both E.Miller-at-curtin.edu.au as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: E.Miller-at-curtin.edu.au } Name: Elaine Miller } } Organization: Curtin University of Technology, Perth, W Australia } } Title-Subject: [Filtered] Coating for FIBSEM } } Question: We recently have acquired a Zeiss Neon FIBSEM with FESEM } capabilities. I was wondering if anyone could give me any advice on a } suitable coating unit. We have to replace our old gold sputter coater } so I was hoping to buy something that could do both high resolution } (platinum?) coatings and regular gold coats for SEM. I have read } about Osmium plasma coating but that is beyond our means at the } moment and I am a bit worried about the OHS issues. Is platinum the } way to go? Does anyone have any good/bad experiences to point me in } any particular direction before I commit to a purchase? } } } Login Host: 203.59.88.207 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu Nov 20 07:42:26 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAKDgQuL021811 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 20 Nov 2008 07:42:26 -0600 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240802c54b19aaf244-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 20 Nov 2008 07:42:25 -0600 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: E.Miller-at-curtin.edu.au (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Coating for FIBSEM } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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hello to All First, thanks to all of you who responded to my question about image acquisition and analysis software. I apologize for the delayed response on my part...major equipment failures have kept me otherwise occupied....
Here is a summary of sorts: The 12 responses I received from List members (not sales reps) listed a total of 6 other software packages. By far, the 3 that got the most mention were:
Image Pro Plus (Media Cybernetics), [and the Fovea Pro 4 package from Reindeer Graphics] Volocity (Improvision) and SlideBook (Intelligent Imaging Innovations).
Also mentioned were: Nikon NIS Elements ( Nikon), and Open Labs (Improvision).
Packages for analysis alone: Imaris Huygens (Scientific Volume Imaging)
No surprise, most people were happiest with the software they knew best, although there was certainly enough food for thought. I thank you all again, Lee -- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
There is also the 'England Finder' where you replace the slide rather than the objective to give the object of interest location [as an XY co-ordinate]. The slide contains a detailed etched grid on the glass and is more accurate than the red printed circle replacement 'objective' method [never tried the diamond scribe version]. Details of the England Finder are at:
I do have a pdf somewhere discussing methods of re-locating an area of interest on a standard slide or Petri Dish. The major downside with the England finder is that when removing the specimen slide, the stage can slide about [older microscopes used to have a locking screw for the stage, but you can use a plastic clamp and a couple of glass slides to lock the stage].
The printed red ring 'objective' replacement system major downsides are that the ink wipes off easily and it's no use with wet mountants such as vecta-shield as the cover-slips moves so easily, and high power oil immersion dissolves the ink. Plus the England Finder never obscures any area of the slide, and you can locate twenty or more objects of interest on one slide [albeit very very slowly]. There are etched grid cover slip versions that have grids permanently built in for easy & speedy re-location, and these are sold fitted to Petri dishes bottoms [e.g. Mattek].
Using dry air objectives, the switched red-ring printing 'objective' is far far easier and faster to use than the England Finder though, as you screw it into a spare hole on the nose-piece and switch it in and out as required.
Don't forget the Vernier scale on the stage if the microscopes have one, you can easily do a simple conversion between microscopes different X,Y Vernier scales [particularly if you have that England Finder]. We have previously made our own Vernier scales [cut out of engineer's 6" fine metal rulers] and fitted them to the stage when needed - our workshop did it for us.
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: shawn.galdeen-at-umassmed.edu [mailto:shawn.galdeen-at-umassmed.edu] Sent: 20 November 2008 14:41 To: kjmorris-at-well.ox.ac.uk
John --
We use several of these in the lab to mark fields of view before moving preps from 'scope to 'scope. After a brief survey of the ones we have, I can tell you that they are manufactured both by Zeiss (under the Winkel imprint in our case) and Leica (as Leitz Wetzlar). Sadly, I don't think they are produced any longer, so you're relegated to ebay to find used ones -- and they're pretty rare there, as well. The Leitz ones are pretty simple instruments, and our machine shop has repaired them in the past. If their measurements are complete, I could send them on to you -- assuming you could source the diamond chip that's in the tip of the scribe, a reasonable machine shop should be able to build you something similar, though you may have to lend them one of your objectives so that they could measure thread pitch and diameter. Let me know if you'd like the additional info, and best of luck finding some of these excellent old scribes.
Shawn
Shawn Galdeen, Ph.D. Post-Doctoral Associate Sluder Lab University of Massachusetts Medical School 508.856.8653
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I am trying to locate a diamond scriber that is housed in a modified objective lens. Basically, one locates an area of interest in the light microscope, switches the scriber/objective into position and rotates the objective to scribe a small circle around the area of interest. I believe Zeiss made such a device, but I am wondering if other manufacturers make one was well? -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I'm responding to your questions / comments you posted about the LoMAX low energy x-ray optic for EDS:
To date, FBI labs in Quantico and Photometrics Labs in Huntington Beach, CA have evaluated LoMAX and reported similar results in low energy gain. Info is available at: http://www.parallaxray.com/eds.html (scroll to bottom of page).
The large gains of up to 10X (and more) are possible and routine experience. You are right that the low energy x-rays from the sample are already there, but the detector only collects x-rays within the solid angle between it and the sample. What LoMAX does is collect x-rays from a much larger solid angle and reflect them back towards the detector window. Lower energies are more efficiently reflected than higher energies and above 1000eV, there is no gain using LoMAX. So, Be would see a 10X gain and O about a 2X gain.
Some limitations / comments:
If the detector already has a large solid angle, such as 50mm2 or larger, then the relative gain from LoMAX is negligible and not worth it, unless one wants to use it as a low-pass filter for an element, or group of light elements. The best gain is obtained using a 10mm2 detector, but a 30mm2 detector would have about half that gain.
In some geometries, the EDS must be retracted away from the sample to accommodate LoMAX optic. The loss in solid angle for higher energy elements can be compensated for by using higher electron beam current. Energies above 1000eV are not affected by LoMAX. A regular collimator can be inserted when the optic is not being used.
Most standardless quant programs will be affected by the higher gain with LoMAX for sub-1000eV x-rays. Some programs allow the user to adjust these sensitivity / calibration factors. Otherwise, the analysis will need calibration standards to be quantitative. This is the case with light elements with many 'standardless' programs anyway.
For more detailed data go to: http://www.parallaxray.com/images/Microscopy%20Today%20Article%20LoMAX%20EDS %20Optic.pdf.
In short, this takes a $30 -$50K EDS system and opens up the whole world of light elements to where it can be done more quantitatively and with higher sensitivity, or with shorter acquisitions.
I may be reached at email, phone, Skype below.
Regards,
Don
Don Kloos VP Sales, Marketing, Business Development
-----Original Message----- X-from: jmcclin-at-uky.edu [jmcclin-at-uky.edu] Sent: Tuesday, November 18, 2008 6:16 PM To: joexray-at-cinci.rr.com
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Email: jmcclin-at-uky.edu Name: Joel McClintock
Organization: U of Kentucky
Title-Subject: [Filtered] SEM EDX Light Element Amplification
Question: We recently had a representative for Parallax Research come in and show us the new LoMAX focused optic product.
The paper presented shows that the product amplifies the light element range of an EDX detector and allows us to speed up mapping of light elements and find trace light elements in samples where the detectors straight from the manufacturer fail to do so in many applications. The price for the unit and installation is pretty reasonable, does anyone have one of these yet and what have your results with the attachment proven for you?
The explanation for the increase was based on a diffraction principle, how does this work?
It does not seem feasible that such an attachment would increase the signal for light elements by as much as 10x if the x-rays for the light elements were already there for the EDX detector to detect to start with. What are the drawbacks of this product?"
If this works at low eV, how does it affect high eV results? Does this mean that one must use the attachment for low eV for certain samples and then remove it for higher Z samples? If so, how does ZAF quant fit into this scenario?
gary g.
At 02:11 PM 11/19/2008, you wrote:
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==============================Original Headers============================== 8, 21 -- From gary-at-gaugler.com Fri Nov 21 22:40:03 2008 8, 21 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAM4e3cg016645 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 21 Nov 2008 22:40:03 -0600 8, 21 -- Message-Id: {200811220440.mAM4e3cg016645-at-ns.microscopy.com} 8, 21 -- Received: (qmail 29071 invoked from network); 21 Nov 2008 20:22:40 -0800 8, 21 -- Received: by simscan 1.1.0 ppid: 29067, pid: 29069, t: 0.1420s 8, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 8, 21 -- by smtp1 with SMTP; 21 Nov 2008 20:22:40 -0800 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Fri, 21 Nov 2008 20:39:56 -0800 8, 21 -- To: joexray-at-cinci.rr.com 8, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 21 -- Subject: Re: [Microscopy] Re: [Filtered] MicroscopyListserverviaWWW: 8, 21 -- SEM EDX 8, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 21 -- In-Reply-To: {200811192211.mAJMB2AC018186-at-ns.microscopy.com} 8, 21 -- References: {200811192211.mAJMB2AC018186-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Email: scott.streiker-at-udri.udayton.edu Name: Scott Streiker
Organization: University of Dayton Research Institute
Title-Subject: [Filtered] DV-502 manual request.
Question: Thanks to all who generously responded to recent request for a Denton Vacuum operarting manual. Copy of the manual graciously offed and has been acquired. The required and repairs are now able to proceed, Cheers, Scott Streiker Associate Research Microscopist Nanoscale Enginnering Science and Technology (NEST)Lab University of Dayton Research Institute
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Email: william.oxberry-at-downstate.edu Name: William Oxberry
Organization: SUNY Downstate
Title-Subject: [Filtered] need opinions for new confocal
Question: I oversee a confocal core lab that has a Bio Rad MRC 1024 Kr/Ar CLSM on an Olympus IX70 inverted research microscope. We are considering upgrading to a new system. Most users utilize 3 channels for imaging, a red and green probe, with a nuclear counterstain in the far red(topro3) Most of the samples are fixed cells or tissues. Most users are Neuroscientists and look at brain slices and cultured neurons. We have not done time lapse, but do 3D stacks and projections and analyse for co localization. Question is: Should we consider a laser scanning system or spinning disk? Cost is a factor for both purchase and maintenance. Laser replacement just cost over $4000 (third one) and now power supply needs repair. Ten months ago $12500 for PMT. Parts and support have become limited and expensive.
The spinning disk systems with mercury lamps sound appealing. How are the latest models performing? If you have one, are you happy with it? For labs that have both types, which would you recommend (if you could only get one) for most general use and flexibility? What is the difference in cost of laser scanning vs spinning disk?
Thanks in advance for advice.
William C Oxberry, support specialist Core confocal facility- BSB4-130 SUNY Downstate Medical Center Brooklyn, NY, 11203 Phone 718-270-4472 william.oxberry-at-downstate.edu
The unit does not affect the higher KV spectra or results when properly installed and aligned. A larger portion of x-rays get placed into the light element region causing the deadtime and count rat to rise, dependent on how much light element data is gained form the sample, however the heavier element spectra is not changed in their ratios to one another, and there are no artifacts from the optic than I have seen.
The optic can stay attached 100% of the time, however since the optic is only 4mm from the sample in operation and does suffer in very low mag from having low peripheral "vision" the analyst may wish to change to the supplied collimator, especially for samples with high surface topography.
As far as quantitiative analysis, if the light element range is not included in the analysis there is no change in results or setup, since the light elements are attenuated if they are included in the mix the user must run and use standards from the EDS platform to get good results, actually by doing so will give better results for light element quant than using standards without the LoMAX, as the higher statistics allow for better analysis.
I will be doing demonstrations of the LoMAX at selected sites in the eastern half of the USA over the next two or three months, stay in touch with my website to find one near you. (www.joexray.net)
Don Kloos of Parallax Research is covering the western portion of the USA, and has others overseas to cover the rest of the world. Go to the Parallax website at www.parallaxray.com to get info on areas outside of the eastern USA.
Thank you,
Joe Ullmer ---- Gary Gaugler {gary-at-gaugler.com} wrote: } If this works at low eV, how does it affect high eV } results? Does this mean that one must use the attachment } for low eV for certain samples and then remove it for } higher Z samples? If so, how does ZAF quant fit into } this scenario? } } gary g. } } } At 02:11 PM 11/19/2008, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Joel, and listers/listees: } } } } The Parallax Research LoMAX that attaches to the EDS detectors from } } virtually any MFG utilizes a focused optic that is highly reflective } } and sets close to the sample, much like the optics used by Parallax, } } Thermo, and EDAX with their parallel beam WDS systems. (Parallax is } } the source for the optics used by all three with the exception of } } the hybrid that Thermo uses to get a larger energy range, which is } } still part Parallax) } } } } This optic does not absorb or allow for the high percentage of } } losses in the light element range due to the fact that the optic is } } "tuned" to diffract or reflect the wavelength of the energy range of } } 100 to about 900 eV. Without the LoMAX the x-rays have to be } } directly going towards the detector window or they are lost, the } } lower the energy, the easier they are lost due to direction and/or absorbance. } } } } I have been working with Parallax to get the product ready and } } insure that it fits on most all 10mm and 30mm SiLi and 10mm SDD } } detectors since March. The last revision was tested on September } } 26th at UES and Parallax now offers the LoMAX to attach to the } } majority of SEM / EDS combinations. The UES report and a pic of the } } LoMAX is on the JoeXray website. } } } } We also tested the LoMAX on a SEM/EDS combination at Case Western } } Reserve University last week and tested for artifacts and quant } } results. Using a Cr-Ni standard the cr and ni did not change when } } the O from the oxidized surface was not in the setup, when it was } } the O went from 1.4% to 4.3% using standardless quant on the NORAN } } System Six, showing that the EDS MFG standardless would not be } } appropriate for light elements in the mix, standards would be } } necessary. However the heavier SQ was not affected when the O was } } not in, which is good. The LoMAX comes with a Parallax provided } } collimator to allow the user to go back to "normal state" while } } keeping the calibrated adapter / trap in place to prevent the need } } for re-calibration when swapping from collimator to the LoMAX optic } } } } Feel free to visit the JoeXray website (www.joexray.net) to see more } } and you may also go to the Parallax Research website as well at } } www.parallaxray.com. } } } } Thank you, } } } } Joe Ullmer } } } } JoeXray LLC } } 7958 Dubois Road } } Carlisle, OHIO 45005 } } OFFICE / FAX: 937 550-9224 } } Cell: 937 520-3811 } } joexray-at-cinci.rr.com } } www.joexray.net } } } } } } ---- jmcclin-at-uky.edu wrote: } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so } } when replying } } } please copy both jmcclin-at-uky.edu as well as the MIcroscopy Listserver } } } --------------------------------------------------------------------------- } } } } } } Email: jmcclin-at-uky.edu } } } Name: Joel McClintock } } } } } } Organization: U of Kentucky } } } } } } Title-Subject: [Filtered] SEM EDX Light Element Amplification } } } } } } Question: We recently had a representative for Parallax Research come } } } in and show us the new LoMAX focused optic product. } } } } } } The paper presented shows that the product amplifies the light } } } element range of an EDX detector and allows us to speed up mapping of } } } light elements and find trace light elements in samples where the } } } detectors straight from the manufacturer fail to do so in many } } } applications. The price for the unit and installation is pretty } } } reasonable, does anyone have one of these yet and what have your } } } results with the attachment proven for you? } } } } } } The explanation for the increase was based on a diffraction } } } principle, how does this work? } } } } } } It does not seem feasible that such an attachment would increase the } } } signal for light elements by as much as 10x if the x-rays for the } } } light elements were already there for the EDX detector to detect to } } } start with. What are the drawbacks of this product?" } } } } } } } } } Thank you all. } } } } } } } } } } } } Joel McClintock } } } } } } EM Specialist } } } } } } U of Kentucky } } } } } } Lexington, Ky } } } } } } } } } } } } Login Host: 74.140.182.126 } } } --------------------------------------------------------------------------- } } } } } } ==============================Original } } Headers============================== } } } 19, 12 -- From zaluzec-at-microscopy.com Tue Nov 18 20:09:08 2008 } } } 19, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22]) } } } 19, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mAJ2955p029816 } } } 19, 12 -- for {microscopy-at-microscopy.com} ; Tue, 18 Nov 2008 } } 20:09:08 -0600 } } } 19, 12 -- Mime-Version: 1.0 } } } 19, 12 -- Message-Id: {p06240806c549259a2b32-at-[206.69.208.22]} } } } 19, 12 -- Date: Tue, 18 Nov 2008 20:09:05 -0600 } } } 19, 12 -- To: microscopy-at-microscopy.com } } } 19, 12 -- From: jmcclin-at-uky.edu (by way of MicroscopyListserver) } } } 19, 12 -- Subject: [Filtered] MicroscopyListserverviaWWW: SEM EDX } } Light Element } } } 19, 12 -- Amplification } } } 19, 12 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } } ==============================End of - } } Headers============================== } } } } -- } } Joe Ullmer } } } } } } } } } } } } ==============================Original Headers============================== } } 16, 21 -- From joexray-at-cinci.rr.com Wed Nov 19 16:09:12 2008 } } 16, 21 -- Received: from hrndva-omtalb.mail.rr.com } } (hrndva-omtalb.mail.rr.com [71.74.56.125]) } } 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mAJM9B3U015744 } } 16, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Nov 2008 } } 16:09:12 -0600 } } 16, 21 -- Received: from hrndva-web14-z01 ([10.128.132.105]) } } 16, 21 -- by hrndva-smta01.mail.rr.com with ESMTP } } 16, 21 -- id } } {20081119220911.LFIG24887.hrndva-smta01.mail.rr.com-at-hrndva-web14-z01} ; } } 16, 21 -- Wed, 19 Nov 2008 22:09:11 +0000 } } 16, 21 -- Message-ID: {20081119220911.ASRWT.73850.root-at-hrndva-web14-z01} } } 16, 21 -- Date: Wed, 19 Nov 2008 17:09:11 -0500 } } 16, 21 -- From: {joexray-at-cinci.rr.com} } } 16, 21 -- To: jmcclin-at-uky.edu, "Microscopy-at-microscopy.com" } } {Microscopy-at-microscopy.com} } } 16, 21 -- Subject: Re: [Microscopy] [Filtered] } } MicroscopyListserverviaWWW: SEM EDX } } 16, 21 -- Light Element } } 16, 21 -- In-Reply-To: {200811190215.mAJ2FksP010474-at-ns.microscopy.com} } } 16, 21 -- MIME-Version: 1.0 } } 16, 21 -- Content-Type: text/plain; charset=utf-8 } } 16, 21 -- Content-Transfer-Encoding: 7bit } } 16, 21 -- X-Priority: 3 (Normal) } } 16, 21 -- Sensitivity: Normal } } 16, 21 -- X-Originating-IP: } } ==============================End of - Headers============================== }
As many of you are aware, I edit the NetNotes column for the Microscopy Society of America's Microscopy Today journal. This column includes the most interesting questions and answers posted to the Microscopy Listserver. Recently there has been a proliferation of postings by individuals with legal statements at the bottom of the posting saying things such as "The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited." That is the poster's prerogative but seems silly when posting a comment to a public listserver. The bottom line is that if your posting includes this type of statement, I will not include your question or answer in the NetNotes column. I don't have time to write for permission each time so I will simply ignore those postings. It is unfortunate that some good threads are lost because of this so if you don't intend for that to be happening, consider removing the statement when you post to the listserver.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Legal statements are automatically added by e-mail clients, such as Outlook, to the messages sent from corporate e-mail accounts. Many users, who use there's work e-mail to read and post on listserver, have no control (administrative privileges on there's computers are blocked) and no choice over content and the very fact of addition of these legal statements to outgoing messages.
Use of private e-mail addresses for subscribing to listserver may be a voluntarily alternative, which could also ease barrage of "out of office" responses everyone receives to his/her posting, but I realize that added inconvenience makes this suggestion impractical for many.
Cheers, Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Saturday, November 22, 2008 5:08 PM To: vray-at-partbeamsystech.com
As many of you are aware, I edit the NetNotes column for the Microscopy Society of America's Microscopy Today journal. This column includes the most interesting questions and answers posted to the Microscopy Listserver. Recently there has been a proliferation of postings by individuals with legal statements at the bottom of the posting saying things such as "The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited." That is the poster's prerogative but seems silly when posting a comment to a public listserver. The bottom line is that if your posting includes this type of statement, I will not include your question or answer in the NetNotes column. I don't have time to write for permission each time so I will simply ignore those postings. It is unfortunate that some good threads are lost because of this so if you don't intend for that to be happening, consider removing the statement when you post to the listserver.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vavv2012-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: vavv2012-at-hotmail.com Name: Arnold Villanueva
Organization: none
Title-Subject: [Filtered] academic programs in colleges/universities in Microscopy
Question: To whom it may concern: I am wondering if there is ANY college and/or university and/or technical or vocational school that offers either a certificate, Associate's Degree or Bachelor's degree program in the field of Microscopy. ANY information that you can offer would be very much appreciated :) Thank you and have a nice day.
I think that there are (at least) two parts to this question.
One is the technical aspect of the subject while the other is the personnal facet. These in actuality really merge into one holistic capability and a personal view of the micro world. So, blah, blah, blah. The point is that if you do not get the technical material, you will fail. Secondarily, if you do not get the reasons for doing EM, why bother?
I used to recommend Univ of Pacific at Delta College. This was in Stockton, CA. But with Judy Murphy gone, I am hesitant to recommend this establishment. It appears that the campus whackers have made a very viable and valuable venue next to nothing of value...or so it seems.
Caveat emptor.
gary g.
At 07:09 PM 11/23/2008, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 21 -- From gary-at-gaugler.com Sun Nov 23 23:33:15 2008 11, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAO5XFG7017644 11, 21 -- for {microscopy-at-microscopy.com} ; Sun, 23 Nov 2008 23:33:15 -0600 11, 21 -- Message-Id: {200811240533.mAO5XFG7017644-at-ns.microscopy.com} 11, 21 -- Received: (qmail 20115 invoked from network); 23 Nov 2008 21:32:45 -0800 11, 21 -- Received: by simscan 1.1.0 ppid: 20112, pid: 20113, t: 0.1063s 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 21 -- by smtp2 with SMTP; 23 Nov 2008 21:32:45 -0800 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Sun, 23 Nov 2008 21:33:13 -0800 11, 21 -- To: vavv2012-at-hotmail.com 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 21 -- Subject: Re: [Microscopy] viaWWW: academic programs in 11, 21 -- colleges/universities in Microscopy 11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 21 -- In-Reply-To: {200811240309.mAO39Tjc001086-at-ns.microscopy.com} 11, 21 -- References: {200811240309.mAO39Tjc001086-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
For those of you who are interested in serial section TEM you might be interested in seeing a series of images that were recently acquired by FEI Company using their Helios focussed ion beam microscopes on a resin embedded block of brain tissue that was prepared here at the EPFL. All the images can be downloaded from their site at the following addresses.
The Technical University in Stockholm (KTH) is planning to offer a 2-year masters education in biophysical imaging starting autumn 2009. This will include light and electron microscopy, X-ray and electron crystallography, structural biochemistry and cell biology, mathematics of Fourier-transforms, Radon-transforms etc. plus a few other topics such as entrepreneurship, biomedical ethics, philosophy of science and some basic physics (electrodynamics, optics, quantum mechanics).
The program is aimed at students with a bachelors degree in physics, engineering (electrical, chemical etc.), mathematics or computer science.
All the courses are taught by researchers at the KTH and the Karolinska Institute.
Contact me for more information if your interested.
Philip
-----Original Message----- X-from: vavv2012-at-hotmail.com [mailto:vavv2012-at-hotmail.com] Sent: 24 November 2008 04:19 To: Philip.Koeck-at-ki.se
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vavv2012-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: vavv2012-at-hotmail.com Name: Arnold Villanueva
Organization: none
Title-Subject: [Filtered] academic programs in colleges/universities in Microscopy
Question: To whom it may concern: I am wondering if there is ANY college and/or university and/or technical or vocational school that offers either a certificate, Associate's Degree or Bachelor's degree program in the field of Microscopy. ANY information that you can offer would be very much appreciated :) Thank you and have a nice day.
Sincerely yours, Arnold Villanueva
==============================Original Headers============================== 16, 21 -- From Philip.Koeck-at-ki.se Mon Nov 24 02:48:41 2008 16, 21 -- Received: from smtp6.ki.se (smtp6.ki.se [130.237.98.104]) 16, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAO8mfed015925 16, 21 -- for {microscopy-at-microscopy.com} ; Mon, 24 Nov 2008 02:48:41 -0600 16, 21 -- Received: from Rapana (rapana.csb.ki.se [130.237.109.49]) 16, 21 -- by smtp6.ki.se (Postfix) with ESMTP id 9DBF4D4AAA; 16, 21 -- Mon, 24 Nov 2008 09:48:40 +0100 (CET) 16, 21 -- From: "Philip Koeck" {Philip.Koeck-at-ki.se} 16, 21 -- To: {vavv2012-at-hotmail.com} , {microscopy-at-microscopy.com} 16, 21 -- References: {200811240319.mAO3JHFG011378-at-ns.microscopy.com} 16, 21 -- Subject: RE: [Microscopy] viaWWW: academic programs in colleges/universities in Microscopy 16, 21 -- Date: Mon, 24 Nov 2008 09:48:30 +0100 16, 21 -- Message-ID: {59B667FBE5E348088575F70AA72A2BF4-at-ad.biosci.ki.se} 16, 21 -- MIME-Version: 1.0 16, 21 -- Content-Type: text/plain; 16, 21 -- charset="us-ascii" 16, 21 -- Content-Transfer-Encoding: 7bit 16, 21 -- X-Mailer: Microsoft Office Outlook 11 16, 21 -- Thread-index: AclN43ATVkMn9nDwSmibKNvg90rXhgAK4+Ag 16, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 16, 21 -- In-reply-to: {200811240319.mAO3JHFG011378-at-ns.microscopy.com} ==============================End of - Headers==============================
There are 3 non-graduate-school academic programs that I know of in the U.S. (I'd like to hear about Canada and Mexico): 2 offer Associates degrees from technical colleges, Delta College in Stockton, CA http://www.deltacollege.edu/dept/electmicro/ and Madison Area Technical College in Madison, WI http://programs.matcmadison.edu/programs/electron-microscopy-technician These are both good programs for training EM techs, and have good reputations. Delta College has recently had a retirement and hired new (experienced) faculty.
There is one program that I know of that offers a B.Sc., ours. It's not a major in microscopy as such, but a Biology major with a concentration in microscopy, and that has separate courses in TEM, SEM, light, and confocal light microscopy. More when there are enough students to offers more. We do welcome non-biology majors in these courses. http://microscopy.bio.cmich.edu/class.html
There are various graduate programs and courses, the one I'm fond of (I went through it), is taught at Iowa State U. in the Bessey Microscopy Facility: http://www.biotech.iastate.edu/publications/service_facilities/microscopy.html (Intense.) I'm sure there are others, but as organized microscopy programs? I think Steve Barlow is collecting information on this, hopefully he'll respond with a list.
Other than that, there are several schools that offer survey courses and intense short-course workshops in either general microscopy or specific kinds of microscopy. The latter usually assume some background in microscopy.
Phil
} Email: vavv2012-at-hotmail.com } Name: Arnold Villanueva } } Organization: none } } Title-Subject: [Filtered] academic programs in colleges/universities } in Microscopy } } Question: To whom it may concern: } I am wondering if there is ANY college and/or university and/or } technical or vocational school that offers either a certificate, } Associate's Degree or Bachelor's degree program in the field of } Microscopy. ANY information that you can offer would be very much } appreciated :) Thank you and have a nice day. } } Sincerely yours, } Arnold Villanueva
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 8, 27 -- From oshel1pe-at-cmich.edu Mon Nov 24 07:45:32 2008 8, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAODjWvM005302 8, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Nov 2008 07:45:32 -0600 8, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 8, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mAODjNlk024876; 8, 27 -- Mon, 24 Nov 2008 08:45:27 -0500 8, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 8, 27 -- Mon, 24 Nov 2008 08:45:11 -0500 8, 27 -- Mime-Version: 1.0 8, 27 -- Message-Id: {f06240800c5505d3d7586-at-[141.209.160.249]} 8, 27 -- In-Reply-To: {200811240314.mAO3En3A004862-at-ns.microscopy.com} 8, 27 -- References: {200811240314.mAO3En3A004862-at-ns.microscopy.com} 8, 27 -- Date: Mon, 24 Nov 2008 08:45:08 -0500 8, 27 -- To: vavv2012-at-hotmail.com 8, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 8, 27 -- Subject: Re: [Microscopy] viaWWW: academic programs in 8, 27 -- colleges/universities in Microscopy 8, 27 -- Cc: Microscopy-at-microscopy.com 8, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 8, 27 -- X-OriginalArrivalTime: 24 Nov 2008 13:45:11.0235 (UTC) FILETIME=[DEF3A930:01C94E3A] 8, 27 -- X-Canit-CHI2: 0.00 8, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 8, 27 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 8, 27 -- X-CanItPRO-Stream: default 8, 27 -- X-Canit-Stats-ID: 5713193 - 29ee3cb77016 8, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The MATC (http://matcmadison.edu/electronmicros/) Madison Area Technical College 3550 Anderson St. Madison, WI 53704 608-246-6100
San Joaquin DELTA College (http://www.deltacollege.edu/dept/electmicro/)
San Joaquin Delta College 5151 Pacific Ave Stockton, CA 95207 209-954-5151
Both of which offer degree programs
There is also
The College of Microscopy (http://www.collegeofmicroscopy.com/) The McCrone Group 850 Pasquinelli Drive Westmont, IL 60559
I donot believe the CoM offers a degree program, it does offer speciality training courses in all forms of microscopy. It is IACET accredited to offer continuing education units.
Likely there are others that will pop up in replies to your posting.
Nestor
At 9:07 PM -0600 11/23/08, vavv2012-at-hotmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 19, 14 -- From zaluzec-at-microscopy.com Mon Nov 24 07:48:11 2008 19, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 19, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAODmAkr008231; 19, 14 -- Mon, 24 Nov 2008 07:48:10 -0600 19, 14 -- Mime-Version: 1.0 19, 14 -- Message-Id: {p06240807c5505f0d3bbe-at-[206.69.208.22]} 19, 14 -- In-Reply-To: {200811240307.mAO37N3c030952-at-ns.microscopy.com} 19, 14 -- References: {200811240307.mAO37N3c030952-at-ns.microscopy.com} 19, 14 -- Date: Mon, 24 Nov 2008 07:48:08 -0600 19, 14 -- To: vavv2012-at-hotmail.com, microscopy-at-microscopy.com 19, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 19, 14 -- Subject: Re: viaWWW: academic programs in colleges/universities in 19, 14 -- Microscopy 19, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Regrettably, Valery's comment applies to our corporate email system. The message sent with every email reads in part:
" This message may contain information that is confidential and/or protected by law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or communication of this message is strictly prohibited. If you have received this communication in error, please contact the sender immediately and delete the message."
There is no way for me to remove or alter this message. However, I could attach an additional statement to my signature similar to this when sending a listserv message, if it would help:
"This message is intended for the Microscopy Listserv. Permission is specifically granted to the Microscopy Society of America to publish some or all of this message in the Microscopy Today journal."
How does that sound to everyone?
Thanks,
Robert Zonis Technical Service, LMTC Sanford L.P. - A Newell Rubbermaid Company robert.zonis-at-sanford.com
Legal statements are automatically added by e-mail clients, such as Outlook, to the messages sent from corporate e-mail accounts. Many users, who use there's work e-mail to read and post on listserver, have no control (administrative privileges on there's computers are blocked) and no choice over content and the very fact of addition of these legal statements to outgoing messages.
Use of private e-mail addresses for subscribing to listserver may be a voluntarily alternative, which could also ease barrage of "out of office" responses everyone receives to his/her posting, but I realize that added inconvenience makes this suggestion impractical for many.
Cheers, Valery Ray
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==============================Original Headers============================== 19, 34 -- From Robert.Zonis-at-Sanford.com Mon Nov 24 12:43:45 2008 19, 34 -- Received: from mail192.messagelabs.com (mail192.messagelabs.com [216.82.241.243]) 19, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAOIhe8U020731 19, 34 -- for {Microscopy-at-Microscopy.Com} ; Mon, 24 Nov 2008 12:43:42 -0600 19, 34 -- X-VirusChecked: Checked 19, 34 -- X-Env-Sender: Robert.Zonis-at-Sanford.com 19, 34 -- X-Msg-Ref: server-8.tower-192.messagelabs.com!1227552202!2633570!4 19, 34 -- X-StarScan-Version: 6.0.0; banners=sanford.com,-,- 19, 34 -- X-Originating-IP: [198.176.16.26] 19, 34 -- Received: (qmail 25426 invoked from network); 24 Nov 2008 18:43:24 -0000 19, 34 -- Received: from naehub1.newellco.com (HELO naehub1.newellco.com) (198.176.16.26) 19, 34 -- by server-8.tower-192.messagelabs.com with SMTP; 24 Nov 2008 18:43:24 -0000 19, 34 -- Received: from naoaksasebe02.nr.ad.newellco.com ([10.217.158.64]) by naehub1.newellco.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 34 -- Mon, 24 Nov 2008 12:43:21 -0600 19, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 34 -- Content-class: urn:content-classes:message 19, 34 -- MIME-Version: 1.0 19, 34 -- Content-Type: text/plain; 19, 34 -- charset="us-ascii" 19, 34 -- Subject: RE: [Microscopy] RE: Confidentiality notices on email postings 19, 34 -- Date: Mon, 24 Nov 2008 12:41:03 -0600 19, 34 -- Message-ID: {66260BA266051B4FA0EC9EA3B33E6A94B6E6AE-at-naoaksasebe02.nr.ad.newellco.com} 19, 34 -- In-Reply-To: {200811230944.mAN9iQ1w006839-at-ns.microscopy.com} 19, 34 -- X-MS-Has-Attach: 19, 34 -- X-MS-TNEF-Correlator: 19, 34 -- Thread-Topic: [Microscopy] RE: Confidentiality notices on email postings 19, 34 -- Thread-Index: AclNUBUFId7lbbaoThWyR4LODGCqugBEbWaw 19, 34 -- References: {200811230944.mAN9iQ1w006839-at-ns.microscopy.com} 19, 34 -- From: "Zonis, Robert" {Robert.Zonis-at-Sanford.com} 19, 34 -- To: {vray-at-partbeamsystech.com} , {PhillipsT-at-missouri.edu} , 19, 34 -- {Microscopy-at-Microscopy.Com} 19, 34 -- X-OriginalArrivalTime: 24 Nov 2008 18:43:21.0962 (UTC) FILETIME=[86A7F4A0:01C94E64] 19, 34 -- Content-Transfer-Encoding: 8bit 19, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mAOIhe8U020731 ==============================End of - Headers==============================
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: Zonis, Robert [mailto:Robert.Zonis-at-Sanford.com] Sent: Monday, November 24, 2008 12:41 PM To: vray-at-partbeamsystech.com; Phillips, Thomas E.; Microscopy-at-Microscopy.Com
Sounds good to me.
Phil
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-- Philip Oshel Technical Editor, Microscopy Today Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 25 -- From oshel1pe-at-cmich.edu Mon Nov 24 13:15:06 2008 4, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAOJF4WL007155 4, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Nov 2008 13:15:05 -0600 4, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 25 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mAOJF1bl029160 4, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Nov 2008 14:15:04 -0500 4, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 25 -- Mon, 24 Nov 2008 14:15:01 -0500 4, 25 -- Mime-Version: 1.0 4, 25 -- Message-Id: {f06240803c550ad42501c-at-[141.209.160.249]} 4, 25 -- In-Reply-To: {200811241906.mAOJ67bx000573-at-ns.microscopy.com} 4, 25 -- References: {200811241906.mAOJ67bx000573-at-ns.microscopy.com} 4, 25 -- Date: Mon, 24 Nov 2008 14:14:58 -0500 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 25 -- Subject: Re: [Microscopy] Confidentiality notices on email postings 4, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 25 -- X-OriginalArrivalTime: 24 Nov 2008 19:15:01.0473 (UTC) FILETIME=[F2DA5510:01C94E68] 4, 25 -- X-Canit-CHI2: 0.00 4, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 25 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 25 -- X-CanItPRO-Stream: default 4, 25 -- X-Canit-Stats-ID: 5738240 - 05af8dcfb481 4, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The optical cable on an X-Cite unit in our lab failed today. I noticed this morning that the light seemed much dimmer, and on investigation the cable was ~60 degrees C (it hurt a bit to touch at one spot). Has anyone noticed if this sort of failure an abrupt thing, or is it gradual?
If it is a slow change I am wondering if recent experiments might need to be repeated (yet again).
Mike
Michael J. Herron, U of MN, Dept. of Entomology herro001-at-umn.edu 612-624-3688 (office) 612-625-5299 (FAX)
==============================Original Headers============================== 8, 18 -- From herro001-at-umn.edu Mon Nov 24 13:42:00 2008 8, 18 -- Received: from mta-m3.tc.umn.edu (mta-m3.tc.umn.edu [134.84.135.123]) 8, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAOJfxP4029936 8, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Nov 2008 13:41:59 -0600 8, 18 -- Received: from [134.84.48.158] (x84-48-158.cfans.umn.edu [134.84.48.158]) 8, 18 -- by mta-m3.tc.umn.edu (UMN smtpd) with ESMTP 8, 18 -- Mon, 24 Nov 2008 13:41:58 -0600 (CST) 8, 18 -- X-Umn-Remote-Mta: [N] x84-48-158.cfans.umn.edu [134.84.48.158] #+LO+TS+AU+HN 8, 18 -- X-Umn-Classification: local 8, 18 -- Mime-Version: 1.0 (Apple Message framework v753.1) 8, 18 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 18 -- Message-Id: {9F18D336-E4CF-425D-B403-E5F3B3CDF941-at-umn.edu} 8, 18 -- Content-Transfer-Encoding: 7bit 8, 18 -- From: Michael Herron {herro001-at-umn.edu} 8, 18 -- Subject: X-Cite cable failure 8, 18 -- Date: Mon, 24 Nov 2008 13:41:59 -0600 8, 18 -- To: Microscopy-at-microscopy.com 8, 18 -- X-Mailer: Apple Mail (2.753.1) ==============================End of - Headers==============================
The employers seeking microscopists seem to disagree with this pronouncement out of left field that the Delta EM program has been made into "next to nothing of value." Delta students get well-paying jobs before they graduate and keep them and advance after they graduate. This is value: employers are hiring and paying Delta students to work as microscopists.
After 3 1/2 semesters at Delta studying both materials and biological electron microscopy I feel comfortable and knowledgeable using a TEM, SEMs, light microscopes, a variety of microtomes, materials and biological sample preparation equipment, polishing ICs and metals, mixing chemicals, processing tissues, identifying the resulting ultrastructure, identifying tissue types, calibrating electron and light microscopes for magnification and resolution, blah, blah, blah. I can look at a sample and decide what technique to use to analyze it and why, based upon the question being asked, time, costs, available equipment, my teachers' insights, research into the specimen, expected image information. Or at least I know where to start asking questions. With some training I'll also be able to maintain the equipment I use, and learn how to safely and optimally use new equipment to get the information requested. I'll also be able to learn how to use any new type of microscope, because I've been taught how to go about learning to use unfamiliar instruments.
I am getting both an excellent technical education in many aspects of biological and materials electron microscopy and the theoretical background I need to probe the problem, figure out how to approach an answer, and do any additional research necessary.
Is the program perfect? Well, in my opinion, it fails to fully emphasize Cretaceous angiosperm fossils, so it's not perfect, but I'm learning to extrapolate from RAM and stainless to various sedimentary strata, and from extant basal angiosperms in Spurr's and fossil fish and trilobites to the angiosperm fossils I'd rather be studying.
My experience as a student and the incredible amount of technical and theoretical knowledge I've gained over the past 2 years does not translate into "next to nothing of value."
Kleo Pullin San Joaquin Delta College Electron Microscopy Student!
PS I'm also an artist and, if I get nothing else out of the program, it has certainly made my art work more interesting.
--- On Sun, 11/23/08, gary-at-gaugler.com {gary-at-gaugler.com} wrote:
} From: gary-at-gaugler.com {gary-at-gaugler.com} } Subject: [Microscopy] Re: viaWWW: academic programs in } To: KLeoPullin-at-pacbell.net } Date: Sunday, November 23, 2008, 9:38 PM } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I think that there are (at least) two parts to this } question. } } One is the technical aspect of the subject while } the other is the personnal facet. These in } actuality really merge into one holistic capability } and a personal view of the micro world. So, blah, blah, } blah. } The point is that if you do not get the technical } material, you will fail. Secondarily, if you do not } get the reasons for doing EM, why bother? } } I used to recommend Univ of Pacific at Delta College. } This was in Stockton, CA. But with Judy Murphy gone, } I am hesitant to recommend this establishment. } It appears that the campus whackers have made a very } viable and valuable venue next to nothing of value...or so } it seems. } } Caveat emptor. } } gary g. } } } At 07:09 PM 11/23/2008, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy } Listserver } } using the WWW based Form at } http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a } Subscriber, so when replying } } please copy both vavv2012-at-hotmail.com as well as } the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: vavv2012-at-hotmail.com } } Name: Arnold Villanueva } } } } Organization: none } } } } Title-Subject: [Filtered] academic programs in } colleges/universities } } in Microscopy } } } } Question: To whom it may concern: } } I am wondering if there is ANY college and/or } university and/or } } technical or vocational school that offers either a } certificate, } } Associate's Degree or Bachelor's degree program } in the field of } } Microscopy. ANY information that you can offer would } be very much } } appreciated :) Thank you and have a nice day. } } } } Sincerely yours, } } Arnold Villanueva } } } } Login Host: 68.7.176.201 } } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } } 7, 11 -- From zaluzec-at-microscopy.com Sun Nov 23 } 21:07:22 2008 } } 7, 11 -- Received: from [206.69.208.22] } (mac22.zaluzec.com [206.69.208.22]) } } 7, 11 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with } } ESMTP id mAO37LHF030934 } } 7, 11 -- for {microscopy-at-microscopy.com} ; } Sun, 23 Nov 2008 } } 21:07:22 -0600 } } 7, 11 -- Mime-Version: 1.0 } } 7, 11 -- Message-Id: } {p06240804c54fcad47db3-at-[206.69.208.22]} } } 7, 11 -- Date: Sun, 23 Nov 2008 21:07:20 -0600 } } 7, 11 -- To: microscopy-at-microscopy.com } } 7, 11 -- From: vavv2012-at-hotmail.com (by way of } MicroscopyListserver) } } 7, 11 -- Subject: viaWWW: academic programs in } colleges/universities } } in Microscopy } } 7, 11 -- Content-Type: text/plain; } charset="us-ascii" ; format="flowed" } } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 11, 21 -- From gary-at-gaugler.com Sun Nov 23 23:33:15 2008 } 11, 21 -- Received: from smtp2.mc.surewest.net } (qsmtp.mc.surewest.net [66.60.130.145]) } 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id mAO5XFG7017644 } 11, 21 -- for {microscopy-at-microscopy.com} ; Sun, 23 } Nov 2008 23:33:15 -0600 } 11, 21 -- Message-Id: } {200811240533.mAO5XFG7017644-at-ns.microscopy.com} } 11, 21 -- Received: (qmail 20115 invoked from network); 23 } Nov 2008 21:32:45 -0800 } 11, 21 -- Received: by simscan 1.1.0 ppid: 20112, pid: } 20113, t: 0.1063s } 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 } 11, 21 -- Received: from unknown (HELO thor.gaugler.com) } (66.60.171.211) } 11, 21 -- by smtp2 with SMTP; 23 Nov 2008 21:32:45 -0800 } 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 11, 21 -- Date: Sun, 23 Nov 2008 21:33:13 -0800 } 11, 21 -- To: vavv2012-at-hotmail.com } 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} } 11, 21 -- Subject: Re: [Microscopy] viaWWW: academic } programs in } 11, 21 -- colleges/universities in Microscopy } 11, 21 -- Cc: MSA listserver } {microscopy-at-microscopy.com} } 11, 21 -- In-Reply-To: } {200811240309.mAO39Tjc001086-at-ns.microscopy.com} } 11, 21 -- References: } {200811240309.mAO39Tjc001086-at-ns.microscopy.com} } 11, 21 -- Mime-Version: 1.0 } 11, 21 -- Content-Type: text/plain; } charset="us-ascii"; format=flowed } ==============================End of - } Headers==============================
==============================Original Headers============================== 15, 21 -- From kleopullin-at-pacbell.net Mon Nov 24 23:24:25 2008 15, 21 -- Received: from web83423.mail.sp1.yahoo.com (web83408.mail.sp1.yahoo.com [69.147.64.56]) 15, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAP5OMJZ023303 15, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Nov 2008 23:24:23 -0600 15, 21 -- Received: (qmail 20091 invoked by uid 60001); 25 Nov 2008 05:24:20 -0000 15, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 21 -- s=s1024; d=pacbell.net; 15, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Message-ID; 15, 21 -- b=tBGSAlQAtoNCgQN/ZKtn+6jXJYCv3Xd66FDHIEvw1+VJ+LMR6oByGwSsdTauWU/9+GIioQNFG/lwasQ6m1AzUK2a3ka2OP1JYhxJQ4Sk/TFhMkLlL6mhNClyrsDYZA4IwP41e05BsBPb+Q5eRYqBib9fVyyTdNuGig03GYT3Npc=; 15, 21 -- X-YMail-OSG: wWzKE4AVM1lQ3Ck3dbVyt0YWDJ6Za.AIJiWgq1ErlMl5cl1Wcrfa10k8VoGXRrhwQFxjW2dxLrEbRL7bQF2Zw.1QB4OcZ3rg6ObmWteKi4qpKU_EObk7pgUGd5jRbo1sDiRYDaptahOatdIb1WTkh8NrktoT0pG3NEOo0YemirU7N4Kv5zpfVFkAjP4ZF0YbGk3cWqsDmdm8g6Tif_26lxViH1IEqseK9LjYgyQ3IXN18Jk- 15, 21 -- Received: from [69.225.11.246] by web83408.mail.sp1.yahoo.com via HTTP; Mon, 24 Nov 2008 21:24:20 PST 15, 21 -- X-Mailer: YahooMailWebService/0.7.218.2 15, 21 -- Date: Mon, 24 Nov 2008 21:24:20 -0800 (PST) 15, 21 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 15, 21 -- Reply-To: kleopullin-at-pacbell.net 15, 21 -- Subject: Re: [Microscopy] Re: viaWWW: academic programs in 15, 21 -- To: KLeoPullin-at-pacbell.net, gary-at-gaugler.com, Microscopy-at-microscopy.com 15, 21 -- In-Reply-To: {200811240538.mAO5cKkH026459-at-ns.microscopy.com} 15, 21 -- MIME-Version: 1.0 15, 21 -- Content-Type: text/plain; charset=us-ascii 15, 21 -- Message-ID: {606445.19682.qm-at-web83408.mail.sp1.yahoo.com} ==============================End of - Headers==============================
I may not be really up-to-date but as far as I know, spinning disk microscopy allows the visualization of only the first 400nm of cells. Its main focus is on events taking place at the surface or near the surface of cells (internalization events for example). Its advantage is the very high rate of imaging (ideal for life cell imaging of fast events). The Laser scanning confocal microscope can build 3D images, but at the price of a slower imaging. I would be glad to be correct if needed.
Best regards, Stephane
----- Original Message ---- X-from: "william.oxberry-at-downstate.edu" {william.oxberry-at-downstate.edu} To: nizets2-at-yahoo.com Sent: Saturday, November 22, 2008 3:37:40 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both william.oxberry-at-downstate.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: william.oxberry-at-downstate.edu Name: William Oxberry
Organization: SUNY Downstate
Title-Subject: [Filtered] need opinions for new confocal
Question: I oversee a confocal core lab that has a Bio Rad MRC 1024 Kr/Ar CLSM on an Olympus IX70 inverted research microscope. We are considering upgrading to a new system. Most users utilize 3 channels for imaging, a red and green probe, with a nuclear counterstain in the far red(topro3) Most of the samples are fixed cells or tissues. Most users are Neuroscientists and look at brain slices and cultured neurons. We have not done time lapse, but do 3D stacks and projections and analyse for co localization. Question is: Should we consider a laser scanning system or spinning disk? Cost is a factor for both purchase and maintenance. Laser replacement just cost over $4000 (third one) and now power supply needs repair. Ten months ago $12500 for PMT. Parts and support have become limited and expensive.
The spinning disk systems with mercury lamps sound appealing. How are the latest models performing? If you have one, are you happy with it? For labs that have both types, which would you recommend (if you could only get one) for most general use and flexibility? What is the difference in cost of laser scanning vs spinning disk?
Thanks in advance for advice.
William C Oxberry, support specialist Core confocal facility- BSB4-130 SUNY Downstate Medical Center Brooklyn, NY, 11203 Phone 718-270-4472 william.oxberry-at-downstate.edu
Admittedly, I have old news. What Judy relayed to me on 16 Sept 2004 was that after her arduous work on securing funding for the EM building, the administration wanted to use it for social studies... or some unrelated field from EM. They were not interested in the low volume EM students. After her lengthy fight with the state for funding and finally securing it, a new administration began to tear her plan apart.
There are always two sides to a story. This is the one I got first hand from Judy. If things have changed for the better, please let me know and I will be the first to sing a new tune. A lot could have changed in four years.
gary g.
At 05:15 AM 11/25/2008, you wrote: } Gary, } I am interested in your comment,"It appears that the campus whackers } have made a very viable and valuable venue next to nothing of value...or } so it seems." } Please explain. } I look forward to your input as a student is interested in training at } that school. } Cheers, } Scott Streiker } Associate Research Electron Microscopist } University of Dayton Research Institute } Nanoscale Engineering Science and Technology (NEST) Laboratory } 937-229-5776 } www.nestlaboratory.com } } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Monday, November 24, 2008 12:37 AM } To: Streiker, Scott M. } Subject: [Microscopy] Re: viaWWW: academic programs in } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America
==============================Original Headers============================== 6, 22 -- From gary-at-gaugler.com Tue Nov 25 11:36:06 2008 6, 22 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAPHa6bp005270 6, 22 -- for {microscopy-at-microscopy.com} ; Tue, 25 Nov 2008 11:36:06 -0600 6, 22 -- Message-Id: {200811251736.mAPHa6bp005270-at-ns.microscopy.com} 6, 22 -- Received: (qmail 18872 invoked from network); 25 Nov 2008 09:18:25 -0800 6, 22 -- Received: by simscan 1.1.0 ppid: 18868, pid: 18870, t: 0.1495s 6, 22 -- scanners: regex: 1.1.0 attach: 1.1.0 6, 22 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 6, 22 -- by smtp1 with SMTP; 25 Nov 2008 09:18:25 -0800 6, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 22 -- Date: Tue, 25 Nov 2008 09:36:01 -0800 6, 22 -- To: "Streiker, Scott M." {Scott.Streiker-at-udri.udayton.edu} 6, 22 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 22 -- Subject: RE: [Microscopy] Re: viaWWW: academic programs in 6, 22 -- Cc: MSA listserver {microscopy-at-microscopy.com} 6, 22 -- In-Reply-To: {DD83A71F8A594D418EF56A00E9D816AC05CD2C88-at-RI-ECVS.udri.uday 6, 22 -- ton.edu} 6, 22 -- References: {200811240537.mAO5bJdH024489-at-ns.microscopy.com} 6, 22 -- {DD83A71F8A594D418EF56A00E9D816AC05CD2C88-at-RI-ECVS.udri.udayton.edu} 6, 22 -- Mime-Version: 1.0 6, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Email: bill.mcmanus-at-usu.edu Name: Bill McManus
Organization: Western Dairy Center, Utah State University
Title-Subject: [Filtered] Image J help
Question: We are making gel networks with differing amount of sephadex beads added to the gels. Is there an Image J expert out there who could walk me through the process of taking a Confocal stack and determining the number and volumes of the beads? Also, does anyone know of a method for staining the sephadex beads? At this time we were thinking that we could use their lack of image (black sphere) or auto fluorescence to visualize the beads. Any help would be great.
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Email: osc-at-rti.org Name: Owen Crankshaw
Organization: RTI International
Title-Subject: [Filtered] Image analysis of fibrous mats
Question: We are looking for an image analysis system to automatically measure the sub-micron diameters of fibers in an image of a fibrous mat (several locations on each fiber). Because the fibers in the image are overlapping and randomly oriented, this is a complicated task for an image analyzer. What vendors should I be contacting to inquire about this, and what price range am I looking at for a software system to go on to a pre-existing computer?
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Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: Oxford Laboratory
Title-Subject: [Filtered] used ultramicrotome
Question: I have a co-worker who is interested in purchasing a MT1 ultramicrotome. Does anyone have that they would be willing to sell? Thanks so much
OK Nestor, let me try this again and try to avoid getting it kicked back or the text removed.
McCrone Research Institute, Chicago, IL offers a:
Program for Certification in Applied Chemical Microscopy
The Certification Program provides explicit evaluation of a student's knowledge and capabilities in Applied Chemical Microscopy. There are three general requirements: (1) successful completion of six McRI courses, (2) passing a comprehensive written examination following these courses, and (3) passing a series of practical proficiency trials.
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
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-----Original Message----- X-from: vavv2012-at-hotmail.com [mailto:vavv2012-at-hotmail.com] Sent: Sunday, November 23, 2008 10:16 PM To: ph2-at-sprynet.com
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Email: vavv2012-at-hotmail.com Name: Arnold Villanueva
Organization: none
Title-Subject: [Filtered] academic programs in colleges/universities in Microscopy
Question: To whom it may concern: I am wondering if there is ANY college and/or university and/or technical or vocational school that offers either a certificate, Associate's Degree or Bachelor's degree program in the field of Microscopy. ANY information that you can offer would be very much appreciated :) Thank you and have a nice day.
I don't mean to throw cold water on this but.... are each of these programs accredited? If so, by which accreditation organization? I'm talking about McCrone, Delta College, UC.
This is a big deal for most clients. Many, if not, will pay for an accredited program. The "buy a degree" schemes do not fit. If the organization and degree program are accredited, I would think that they would make a positive issue of that.
But you did not answer Arnold's question "... certificate, Associate's Degree or Bachelor's degree program in the field of Microscopy." I don't know about Delta College...or any others. So, those in the know, please let us and our new persons know.
Just my $1NZ (good exchange rate there last month).
gary g.
At 06:08 PM 11/25/2008, you wrote:
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==============================Original Headers============================== 11, 21 -- From gary-at-gaugler.com Tue Nov 25 20:50:13 2008 11, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mAQ2oDcv017355 11, 21 -- for {microscopy-at-microscopy.com} ; Tue, 25 Nov 2008 20:50:13 -0600 11, 21 -- Message-Id: {200811260250.mAQ2oDcv017355-at-ns.microscopy.com} 11, 21 -- Received: (qmail 11757 invoked from network); 25 Nov 2008 18:49:34 -0800 11, 21 -- Received: by simscan 1.1.0 ppid: 11754, pid: 11755, t: 0.1445s 11, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 21 -- by smtp2 with SMTP; 25 Nov 2008 18:49:34 -0800 11, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 21 -- Date: Tue, 25 Nov 2008 18:50:09 -0800 11, 21 -- To: ph2-at-sprynet.com 11, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 21 -- Subject: Re: [Microscopy] RE: viaWWW: academic programs in 11, 21 -- colleges/universities in Microscopy 11, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 21 -- In-Reply-To: {200811260208.mAQ28J3d005401-at-ns.microscopy.com} 11, 21 -- References: {200811260208.mAQ28J3d005401-at-ns.microscopy.com} 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Smart Imaging Technologies has a ready-to-run application for our SIMAGIS Smart Imaging Workbench that was originally designed to measure nanotube thickness in a buckypaper. It's like looking at a plate of spaghetti because of the intersections, junctions and random orientations.
This application has been referenced in several papers.
You may learn more about this specific SIMAGIS application at the following link: http://smartimtech.com/analysis/modules/module5n.htm
Please feel free to send us a typical image and if our application is suitable we'll prepare a sample report for you using SIMAGIS.
Hope this helps.
Please accept our best wishes for a safe and satisfying holiday weekend.
Regards,
Ira Bleiweiss Smart Imaging Technologies 713.589.3216 direct 877.280.1100 ext.1004 Toll Free (US) ira-at-simagis.us www.smartimtech.com
Advanced Software Solutions for Science and Industry
-----Original Message----- X-from: osc-at-rti.org [mailto:osc-at-rti.org] Sent: Tuesday, November 25, 2008 7:19 PM To: ira-at-simagis.us
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Email: mkarmarkar-at-gmail.com Name: Mak
Organization: virginia tech.
Title-Subject: [Filtered] academic couses in microscopy
Question: Since, this discussion is currently going in this group, I would like to ask a related question about institutes offering Masters and PhD level microscopy courses. Thank you.
Iowa State University offers PhD level courses GDCB 679/680/681, light, SEM, and TEM thorugh its "microscopy and nanoimaging facility". http://www.microscopy.biotech.iastate.edu/services/instruction.html
I'm sure of others, but this is the one I went through.
Phil
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==============================Original Headers============================== 4, 26 -- From oshel1pe-at-cmich.edu Wed Nov 26 08:50:47 2008 4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAQEolBi012605 4, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Nov 2008 08:50:47 -0600 4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mAQEokKf014691; 4, 26 -- Wed, 26 Nov 2008 09:50:46 -0500 4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 4, 26 -- Wed, 26 Nov 2008 09:49:49 -0500 4, 26 -- Mime-Version: 1.0 4, 26 -- Message-Id: {f06240802c55310fbd6f6-at-[141.209.160.249]} 4, 26 -- In-Reply-To: {200811261427.mAQERCbc004387-at-ns.microscopy.com} 4, 26 -- References: {200811261427.mAQERCbc004387-at-ns.microscopy.com} 4, 26 -- Date: Wed, 26 Nov 2008 09:49:48 -0500 4, 26 -- To: mkarmarkar-at-gmail.com 4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 26 -- Subject: Re: [Microscopy] viaWWW: academic couses in microscopy 4, 26 -- Cc: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 26 -- X-OriginalArrivalTime: 26 Nov 2008 14:49:49.0984 (UTC) FILETIME=[3BB14A00:01C94FD6] 4, 26 -- X-Canit-CHI2: 0.00 4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 26 -- X-Spam-Score: -4.40 () [Hold at 5.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001,-0.5) 4, 26 -- X-CanItPRO-Stream: default 4, 26 -- X-Canit-Stats-ID: 5837918 - 47bb47d623d5 4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
But you did not answer Arnold's question "... certificate, Associate's Degree or Bachelor's degree program in the field of Microscopy."
Response:
Actually, I did in my post only - Certificate.
2. Regarding:
" are each of these programs accredited?"
Response:
First, Excellent question, and the most relevant.
Second, define accredited.
US Dept Ed Accredits to a point (financial support purposes; and does have a degree mill webpage about diploma mills), as does ABET. [Go to http://ope.ed.gov/accreditation/ for Dept Ed]
For instance, San Joaquin Delta College is accredited by US Dept of Ed and through the Western Association of Schools and Colleges, Accrediting Commission for Community and Junior Colleges since 1952 [and National League for Nursing Accrediting Commission since 1969].
I went through an ABET* accredited engineering Program (GA Tech), graduating with honors with a Bach in Mechanical Engineering (and an extra 30 or so hours in Env, Chem, Civil, Electrical Eng, and Math). But it did not provide the background for A LOT of the environmental, health and safety, and commercial-industrial forensic work I do and have done since 1986. This includes the Environmental Engineering and Chemical Engineering and Air Pollution Control coursework, etc. As of last count, I've taken 75 additional 1-5 day professional development courses to fulfill this need (20 of them Microscopy-based), and I don't believe any program covers enough to fulfill the basics these days given the breadth of science potentially required in this era. I have tried to carefully select these based on the provider (ex. US EPA, Am Soc Materials, GA Tech Cont Ed, IN Univ, North Carolina, McCrone, etc.) and subject matter. I've decided they each have their place, but they differ from a formal base education in that they specialize more in application. I have tried to take pieces of this application side and put them in my own teaching (at McCrone, at IUPUI in Indianapolis, and through a couple of Organizations).
(I'm getting there)
Secondly, I have a small amount of alphabet soup after my name for various specialty aspects. Each of these has "approval" by the National Council of Examiners for Engineering and Surveying (NCEES); and the Professional Engineering one requires an ABET accreditation or a lot more review and years of experience. I have several other certificates that I do not display, frankly because they don't merit the extra initials and aren't commercially as useful. Each has a minimum requirement, which provides a MINIMUM. As I like to ask,"What do you call a doctor that graduated last in his/her class?" You still call them Doctor.
(I'm getting there)
I have seen differences in engineering school quality in legal cases (having studied, been challenged on and challenged others on Daubert admissibility of expert testimony; and having reviewed investigations and reports of others), even among ABET accredited schools. And I've had fun sitting in the depositions of other "experts" handing questions to the attorneys to ask said experts that are 3-day certificate wonders or diploma mill wonders in a few cases. I have gone so far as to acquire another expert's thesis to check it and the expert for quality and consistency, much to their chagrin.
So the points are:
1. Degrees are fundamental-based learning system approaches. As such they have gaps and limitations, but fulfill a need.
2. Certifications (and certificates) are a minimum-based approach for specialty areas. As such they have gaps and limitations, but fulfill a need.
3. Degrees and Certifications are driven by a strong need. Apparently there isn't a lot for Microscopy only.
[I'll caveat by saying that the demand in a broad-based arena like microscopy makes it difficult to create a good program]
4. Some level of quality control in the form of accreditation is useful and NEEDED, but difficult to achieve in fair practice without some level of bias.
5. Caveat emptor.
And for the Thanksgiving holiday:
Carpe diem.
(in the original context was intended to mean that one should sit back and enjoy the rewards of life, not go out and take on the world)
Disclaimer:
I have no affiliation with Delta but have and continue to teach for McCrone Research Institute [technically I have only a little financial interest as an adjunct faculty and the fact that I lose money by teaching compared to commercial and legal work seems to negate that bias]. I'm also adjunct faculty at IUPUI in Indianapolis.
* They only accredit programs, not whole universities
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: Gary Gaugler [mailto:gary-at-gaugler.com] Sent: Tuesday, November 25, 2008 9:50 PM To: ph2-at-sprynet.com Cc: MSA listserver
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There is an ImageJ listserver: http://rsbweb.nih.gov/ij/list.html
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} } Email: bill.mcmanus-at-usu.edu } Name: Bill McManus } } Organization: Western Dairy Center, Utah State University } } Title-Subject: [Filtered] Image J help } } Question: We are making gel networks with differing amount of } sephadex beads added to the gels. Is there an Image J expert out } there who could walk me through the process of taking a Confocal } stack and determining the number and volumes of the beads? Also, } does anyone know of a method for staining the sephadex beads? At } this time we were thinking that we could use their lack of image } (black sphere) or auto fluorescence to visualize the beads. Any help } would be great. } } Bill McManus } WDC, Utah state University } } Login Host: 129.123.241.53 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Tue Nov 25 19:09:57 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id mAQ19tFw013271 } 7, 11 -- for {microscopy-at-microscopy.com} ; Tue, 25 Nov 2008 } 19:09:56 -0600 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c5525254a735-at-[206.69.208.22]} } 7, 11 -- Date: Tue, 25 Nov 2008 19:09:54 -0600 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: bill.mcmanus-at-usu.edu (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Image J help } 7, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 23 -- From vladislav_speransky-at-nih.gov Wed Nov 26 11:30:01 2008 4, 23 -- Received: from nihrelayxway.hub.nih.gov (mailfwd.nih.gov [128.231.90.106]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAQHU0Mb011000 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Nov 2008 11:30:01 -0600 4, 23 -- X-IronPortListener: NIH_Relay 4, 23 -- X-SBRS: None 4, 23 -- X-IronPort-AV: E=Sophos;i="4.33,670,1220241600"; 4, 23 -- d="scan'208";a="73422516" 4, 23 -- Received: from helix.nih.gov ([128.231.2.3]) 4, 23 -- by nihrelayxway.hub.nih.gov with ESMTP; 26 Nov 2008 12:27:00 -0500 4, 23 -- Received: from db447.niaid.nih.gov (db447.niaid.nih.gov [128.231.217.47]) 4, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id mAQHQtjP006680 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Nov 2008 12:26:56 -0500 4, 23 -- Message-Id: {46B54044-790B-416F-BBA3-5C1E56EE9E3A-at-nih.gov} 4, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 4, 23 -- To: Microscopy-at-microscopy.com 4, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- Mime-Version: 1.0 (Apple Message framework v929.2) 4, 23 -- Subject: Fwd: [Microscopy] viaWWW: Image J help 4, 23 -- Date: Wed, 26 Nov 2008 12:26:55 -0500 4, 23 -- References: {200811260111.mAQ1BEHh016312-at-ns.microscopy.com} 4, 23 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
-----Original Message----- X-from: vavv2012-at-hotmail.com [mailto:vavv2012-at-hotmail.com] Sent: Sunday, November 23, 2008 7:20 PM To: dkloos-at-parallaxray.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vavv2012-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: vavv2012-at-hotmail.com Name: Arnold Villanueva
Organization: none
Title-Subject: [Filtered] academic programs in colleges/universities in Microscopy
Question: To whom it may concern: I am wondering if there is ANY college and/or university and/or technical or vocational school that offers either a certificate, Associate's Degree or Bachelor's degree program in the field of Microscopy. ANY information that you can offer would be very much appreciated :) Thank you and have a nice day.
Dear All, I am looking for "photogenic" specimen in material science, if possible from the actual "leading edge" of the work in this field. Any help is greatly appreciated and images resulting thereof will be handed out for your own use.
I intend to scan in 5000x7000 pixel size, with up to four detectors in use to finally get "coloured" images. Specimen should need not more than 3000 - 5000 magnification to show up pretty because my SEM is resolution-limited to ca. 7nm point to point ;-(
Please see some examples of earlier work at: www.elektronenmikroskopie.info/material
Concerning sending of specimen, please contact me off-list. Specimen might be send back after doing the scans.
Normally you measure the size distribution of the beads/material before you add them to the gel/lung etc.. It's a lot easier and more accurate [all the sample is in one focus plane], and you simply take an aliquot or two from the original stock suspension. You would only want to measure them after they in the gel if you thought they might deform or dissolve in the gel/lung etc.. with time [them you'd undertake a durability study with specific time points]. To measure them in a gel/lung etc.. I'd recover them from the gel by dissolving the gel and filtering through Millipore/Nuclepore membrane filters of appropriate pore size [for SEM & optical] or work out a way of putting them onto a glass slide for optical microscopy [depends on what the beads are made of]. You can just dry the bead suspension 'dropped' onto the slide [in high humidity], but for mineral fibres etc.. we used to use various methods, including 'clearing' the membrane filter [to make it transparent].
Once you have your particulate matter all in one Z plane you no longer have to worry about large z-stacks through the gel and using LD [long working distance objectives] that have inferior imaging qualities to short working distance high NA objectives. This assumes your beads are greater than 0.5um diameter and suitable for measurement by optical microscopy [otherwise it's bead recovery from the gel and SEM - you can recover the beads separately from different layers of the gel if it's necessary to check for uniform distribution]. You could even freeze and cryostat thick section the gel I suppose.
If you must image the beads in the gel [to say check that the size distribution is similar through the gel] I would have thought the only way would be produce a Z stack via an optical microscope [ideally with a confocal microscope, but that won't be crucial] and measure the diameter of the beads at the point where the diameter of the bead is greatest. It's then a simple matter to calculate the volume of an equivalent sphere. This will be a little laborious, but you won't have to measure many beads [50 or so] per gel, assuming they are all broadly the same size. I don't know of any packages that work in 3D to measure this automatically [we have Volocity], all my work has been in 2D and then re-modelled in 3D later [e.g. lung dosimetry]. Measuring the diameter manually might be best, but from my experience with beads they are always pretty easy to image and threshold so semi-automatic measurement should be possible, with manual editing and perhaps object classifiers to remove ones you don't want to measure at that Z plane [too small]. Try phase contrast, and DIC optics [the twin DIC polarising filters can help with Ph phase]. Fluorescence or opaque doping also works well, but optical effects at the surface of standard beads normally means you can detect the edge of the sphere easily even if they are just polystyrene [with optical contrast enhancement]. We always manufactured our own beads, be they radioisotope doped fused alumina-silicate clay or fluorescent/opaque polystyrene, but those facilities are long gone now. Main problem with imaging will be to ensure you only measure each spherical bead at its fattest point for that crucial diameter measurement.
I'm not a real user of imageJ as we have MetaMorph and Volocity here, but I can offer some advice on scoring/measurement if no-one else comes forward - your beads in gel should be easier to sample & measure than the in-vivo recovery of glass/asbestos fibres I used to work on at Harwell (lung durability studies for mineral fibre [glass/rock/ceramic] manufacturers).
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: bill.mcmanus-at-usu.edu [mailto:bill.mcmanus-at-usu.edu] Sent: 26 November 2008 01:19 To: kjmorris-at-well.ox.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bill.mcmanus-at-usu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bill.mcmanus-at-usu.edu Name: Bill McManus
Organization: Western Dairy Center, Utah State University
Title-Subject: [Filtered] Image J help
Question: We are making gel networks with differing amount of sephadex beads added to the gels. Is there an Image J expert out there who could walk me through the process of taking a Confocal stack and determining the number and volumes of the beads? Also, does anyone know of a method for staining the sephadex beads? At this time we were thinking that we could use their lack of image (black sphere) or auto fluorescence to visualize the beads. Any help would be great.
Forgot to add you can use a Coulter Counter or flow cytometer to size and sort your beads as well, provided you use the original bead suspension or recover them from the gel - so that the particles are in a carrier suspension [water & perhaps Tween etc.. not in the gel]. This is a very rapid and accurate way of finding the size distribution of particles provided the laser/light illumination can pick them out [e.g. easy with auto or added fluorescence labels].
Although my PhD is in atmospheric aerosol science [e.g. integrating nephelometers and cloud condensation nuclei CCN counters], flow cytometry's not my area of expertise [mine's light microscopy & image analysis, with a bit of EM], normally I just pass this on to my counterpart in the flow cytometer lab.
For some flow cytometry theory see Wikipedia and search the web http://en.wikipedia.org/wiki/Flow_cytometry http://biology.berkeley.edu/crl/flow_cytometry_basic.html
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: 26 November 2008 17:37 To: kjmorris-at-well.ox.ac.uk
I have studied polymers in TEM and SEM for several years and I have developed methods for studying two phase latexes, polymer blends, rubber materials, asphalt etc. My experiences are summarized in my doctoral thesis in 2004, where I also give an introduction to EM of polymer materials. I send a link to the abstract here
http://www.lu.se/o.o.i.s?id=12588&postid=467641
I still have some ex available if you are interested, send name and address to:
Helen.Hassander-at-polymat.lth.se
Best regards, Helen
==============================Original Headers============================== 9, 23 -- From Helen.Hassander-at-polymat.lth.se Fri Nov 28 09:52:26 2008 9, 23 -- Received: from cassandra.net.lu.se (cassandra.net.lu.se [130.235.61.20]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mASFqPck015783 9, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Nov 2008 09:52:26 -0600 9, 23 -- Received: from net.lu.se (localhost [127.0.0.1]) 9, 23 -- by cassandra.net.lu.se (iPlanet Messaging Server 5.2 HotFix 1.14 (built Mar 18 9, 23 -- 2003)) with ESMTP id {0KB100EM9W1WW7-at-cassandra.net.lu.se} for 9, 23 -- Microscopy-at-microscopy.com; Fri, 28 Nov 2008 16:51:32 +0100 (MET) 9, 23 -- Received: from [90.229.231.124] by cassandra.net.lu.se (mshttpd); Fri, 9, 23 -- 28 Nov 2008 16:51:32 +0100 9, 23 -- Date: Fri, 28 Nov 2008 16:51:32 +0100 9, 23 -- From: Helen Hassander {Helen.Hassander-at-polymat.lth.se} 9, 23 -- Subject: Polymer microscopy 9, 23 -- To: Microscopy-at-microscopy.com 9, 23 -- Message-id: {36839a3621ab.3621ab36839a-at-net.lu.se} 9, 23 -- MIME-version: 1.0 9, 23 -- X-Mailer: iPlanet Messenger Express 5.2 HotFix 1.14 (built Mar 18 2003) 9, 23 -- Content-type: text/plain; charset=us-ascii 9, 23 -- Content-language: sv 9, 23 -- Content-transfer-encoding: 7BIT 9, 23 -- Content-disposition: inline 9, 23 -- X-Accept-Language: sv 9, 23 -- Priority: normal ==============================End of - Headers==============================
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Question: I work with a scanning electron microscope (Cambridge stereoscan 240. I cant find any Polaroid sheet films. Is there any chance that i could use any other (not instant) 4x5 film? If yes which is the one you will recommended to me? Must the film be only black and white, or i can use and color films? Thank you very much.
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... There might still be a possibility to get the Fuji FP-3000B 4x5" sheet film (3000 ASA, you will need to stop down the lens aperture ca. 3.5 stops). Best would be Polaroid Polapan 400 Typ 72, which is still available at some sellers (google "polapan 400 4x5" price"). For ordering in Greece, you might try Spiros Skiadopoulos at the Leica Acadamy Athens. To have a stock you can put the films in the fridge for some years.
What I would do is: going digital with an USB analog / digital converter and a matching programm, grabbing the videosignal of the slowscan... A friend of mine did so on a Philips 515 scope. Images are very nice :-) and up to 4000x4000 pixel size. Is you are doing more than 300 images a year, the system will be paid for after one year... For setup you might need some knowledge finding the appropriate signals on the boards. If you are interested, e-mail me offline.
Best wishes, Stefan Diller
pveril-at-med.uth.gr schrieb: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both pveril-at-med.uth.gr as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: pveril-at-med.uth.gr } Name: Panagiotis Berillis } } Title-Subject: [Filtered] Stereoscan 240 } } Question: I work with a scanning electron microscope (Cambridge } stereoscan 240. I cant find any Polaroid sheet films. Is there any } chance that i could use any other (not instant) 4x5 film? If yes } which is the one you will recommended to me? Must the film be only } black and white, or i can use and color films? } Thank you very much. } } Login Host: 77.49.220.157 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 5, 11 -- From zaluzec-at-microscopy.com Sat Nov 29 23:59:42 2008 } 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mAU5xfon018892 } 5, 11 -- for {microscopy-at-microscopy.com} ; Sat, 29 Nov 2008 23:59:42 -0600 } 5, 11 -- Mime-Version: 1.0 } 5, 11 -- Message-Id: {p06240800c557dc5004dc-at-[206.69.208.22]} } 5, 11 -- Date: Sun, 30 Nov 2008 00:00:08 -0600 } 5, 11 -- To: microscopy-at-microscopy.com } 5, 11 -- From: pveril-at-med.uth.gr (by way of MicroscopyListserver) } 5, 11 -- Subject: viaWWW: Stereoscan 240 } 5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
I was wondering if any of the mineralogist on this list have developed a sample handling and SEM examination protocol for oil shale rock samples. I've only just received an inquery regarding the possibility, but I have never handled such a sample.
Our SEM is capable of environmental chamber pressures (FEI Quanta), but the work will require BEI imaging and EDX spectra. I imagine the vapor pressure from these types of samples can vary from nil to extremes - how would one determine before possibly contaminating the column if any particular sample was going to cause problems?
~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 4, 26 -- From michael-at-shaffer.net Mon Dec 1 09:02:40 2008 4, 26 -- Received: from smtprelay.hostedemail.com (smtprelay0055.hostedemail.com [216.40.44.55]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB1F2bfS016413 4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 09:02:40 -0600 4, 26 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 4, 26 -- by smtprelay01.hostedemail.com (Postfix) with SMTP id 6ED821BCE74E 4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 15:02:36 +0000 (UTC) 4, 26 -- X-SpamScore: 1 4, 26 -- X-Spam-Summary: 50,0,0,13c27218ee358141,0d355e4b7ffc0630,michael-at-shaffer.net,microscopy-at-microscopy.com,RULES_HIT:10:355:379:539:541:542:967:988:989:1155:1160:1260:1277:1311:1313:1314:1345:1437:1515:1516:1518:1534:1539:1593:1594:1711:1730:1747:1766:1792:2378:2393:2525:2560:2563:2682:2685:2693:2857:2859:2933:2937:2939:2942:2945:2947:2951:2954:3022:3352:3636:3740:3865:3866:3867:3868:3869:3871:3876:3877:3934:3936:3938:3941:3944:3947:3950:3953:3956:3959:4250:5007:6114:6261:7679:8501:8526:8985:9010:9025:9388,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 4, 26 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 4, 26 -- (Authenticated sender: michael-at-shaffer.net) 4, 26 -- by omf05.hostedemail.com (Postfix) with ESMTP 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 15:02:35 +0000 (UTC) 4, 26 -- From: "michael shaffer" {michael-at-shaffer.net} 4, 26 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 4, 26 -- Subject: SEM: oil shale sections 4, 26 -- Date: Mon, 1 Dec 2008 11:32:29 -0330 4, 26 -- Message-ID: {B117E0F5690B4EA8863434E19EB5CD00-at-CREAIT.MUN.CA} 4, 26 -- MIME-Version: 1.0 4, 26 -- Content-Type: text/plain; 4, 26 -- charset="us-ascii" 4, 26 -- Content-Transfer-Encoding: 7bit 4, 26 -- X-Mailer: Microsoft Office Outlook 11 4, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 4, 26 -- Thread-Index: AclTxdQu7FaJLHGoRC6lr19bLu8dRg== 4, 26 -- X-session-marker: 6D69636861656C40736861666665722E6E6574 ==============================End of - Headers==============================
Can someone give me the idiot's guide to the differences between active and passive image acquisition solutions for SEM's.
I think I know that passive systems use the scopes controls to steer the beam, active ones add their own scan generator. Maybe someone can flesh out that explanation to include some details (not too complicated) and the advantages and disadvantages of each solution. Can you guess that I have to explain this to a class? My typical arm waving strategy might not work.
Thanks
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 36 -- From jkrupp-at-deltacollege.edu Mon Dec 1 13:12:12 2008 9, 36 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB1JCBBG018025 9, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 13:12:12 -0600 9, 36 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 9, 36 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 9, 36 -- with ESMTP id 43781101 for Microscopy-at-microscopy.com; Mon, 01 Dec 2008 11:12:11 -0800 9, 36 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 9, 36 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 9, 36 -- ESMTP id KB7ONN00.8VS for {Microscopy-at-microscopy.com} ; Mon, 1 9, 36 -- Dec 2008 10:57:23 -0800 9, 36 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C53413D82907 9, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 11:12:10 -0800 (PST) 9, 36 -- X-Virus-Scanned: amavisd-new at 9, 36 -- X-Spam-Flag: NO 9, 36 -- X-Spam-Score: -2.499 9, 36 -- X-Spam-Level: 9, 36 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 9, 36 -- BAYES_00=-2.599, RDNS_NONE=0.1] 9, 36 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 36 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 9, 36 -- with ESMTP id A6m1SP9vD9yG for {Microscopy-at-microscopy.com} ; 9, 36 -- Mon, 1 Dec 2008 11:12:10 -0800 (PST) 9, 36 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) 9, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 77BF83D82906 9, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 11:12:10 -0800 (PST) 9, 36 -- Message-Id: {4E4237E4-5DDE-4957-B0CE-18450F28319B-at-deltacollege.edu} 9, 36 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 9, 36 -- To: Microscopy-at-microscopy.com 9, 36 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 36 -- Content-Transfer-Encoding: 7bit 9, 36 -- Mime-Version: 1.0 (Apple Message framework v929.2) 9, 36 -- Subject: Active vs passive image acquisition 9, 36 -- Date: Mon, 1 Dec 2008 11:12:10 -0800 9, 36 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
The passive system reads the X and Y positions, giving a location, and also reads the Z (video) giving a quantity for the location. Continuing on, it builds the image in memory point by point, line by line. Generally speaking, these are the easiest systems to install because all you do is tap the X, Y and Video signals. This system will also give you any data that your microscope normally writes on the images, so things like mag, kV, WD, etc. are automatically saved in the image.
The active system requires one or more relays to be installed that will connect the SEM (mag, CRTs, etc.) to either the scan generator in the SEM or the scan generator in the active digital imaging system. The active system outputs the X and Y positions to the SEM and reads Z, thereby building an image. Although you lose the data your SEM would normally write on the image, you have more freedom to determine your image size and collection speed. Most SEMs don't scan more than 2000 lines in record mode, but the best systems can take 2000 lines on negative film and do fairly impressive enlargements to 10"x14" or bigger. They can do this because the individual lines are fairly fuzzy and the horizontal portion is usually analog. Going to digital and, say 2kx2k, a 10"x14" is not going to look as good. However, most of the active systems will collect at least up to 4kx4k (some considerably larger) which will match or exceed the best recording systems at 2000 lines.
Of course, an active system also allows an EDS system to look at an area, then go back to specific spots and do further analysis. An active system is also the basis for most EBL (electron beam lithography) systems.
A passive system is a very good (and less expensive) replacement for Polaroid film. An active system will let you go beyond film, but will require more input from the user.
Ken Converse
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Monday, December 01, 2008 2:15 PM To: kenconverse-at-qualityimages.biz
Hi
Can someone give me the idiot's guide to the differences between active and passive image acquisition solutions for SEM's.
I think I know that passive systems use the scopes controls to steer the beam, active ones add their own scan generator. Maybe someone can flesh out that explanation to include some details (not too complicated) and the advantages and disadvantages of each solution. Can you guess that I have to explain this to a class? My typical arm waving strategy might not work.
Thanks
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 36 -- From jkrupp-at-deltacollege.edu Mon Dec 1 13:12:12 2008 9, 36 -- Received: from sjdccd.cc.ca.us (smtp.sjdccd.cc.ca.us [207.62.178.236]) 9, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB1JCBBG018025 9, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 13:12:12 -0600 9, 36 -- Received: from [207.62.178.20] (HELO sunspot.sjdccd.cc.ca.us) 9, 36 -- by sjdccd.cc.ca.us (CommuniGate Pro SMTP 5.0.9) 9, 36 -- with ESMTP id 43781101 for Microscopy-at-microscopy.com; Mon, 01 Dec 2008 11:12:11 -0800 9, 36 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 9, 36 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 9, 36 -- ESMTP id KB7ONN00.8VS for {Microscopy-at-microscopy.com} ; Mon, 1 9, 36 -- Dec 2008 10:57:23 -0800 9, 36 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 9, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id C53413D82907 9, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 11:12:10 -0800 (PST) 9, 36 -- X-Virus-Scanned: amavisd-new at 9, 36 -- X-Spam-Flag: NO 9, 36 -- X-Spam-Score: -2.499 9, 36 -- X-Spam-Level: 9, 36 -- X-Spam-Status: No, score=-2.499 tagged_above=-10 required=6 tests=[AWL=0.000, 9, 36 -- BAYES_00=-2.599, RDNS_NONE=0.1] 9, 36 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 36 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 9, 36 -- with ESMTP id A6m1SP9vD9yG for {Microscopy-at-microscopy.com} ; 9, 36 -- Mon, 1 Dec 2008 11:12:10 -0800 (PST) 9, 36 -- Received: from [172.20.6.52] (unknown [172.20.6.52]) 9, 36 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 77BF83D82906 9, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 11:12:10 -0800 (PST) 9, 36 -- Message-Id: {4E4237E4-5DDE-4957-B0CE-18450F28319B-at-deltacollege.edu} 9, 36 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 9, 36 -- To: Microscopy-at-microscopy.com 9, 36 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 9, 36 -- Content-Transfer-Encoding: 7bit 9, 36 -- Mime-Version: 1.0 (Apple Message framework v929.2) 9, 36 -- Subject: Active vs passive image acquisition 9, 36 -- Date: Mon, 1 Dec 2008 11:12:10 -0800 9, 36 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
==============================Original Headers============================== 25, 25 -- From kenconverse-at-qualityimages.biz Mon Dec 1 14:08:55 2008 25, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 25, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB1K8tq8002126 25, 25 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 14:08:55 -0600 25, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta06.mail.rr.com 25, 25 -- with ESMTP 25, 25 -- id {20081201200854.BCKL20835.cdptpa-omta06.mail.rr.com-at-Ken} ; 25, 25 -- Mon, 1 Dec 2008 20:08:54 +0000 25, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 25 -- To: {jkrupp-at-deltacollege.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 25, 25 -- Subject: RE: [Microscopy] Active vs passive image acquisition 25, 25 -- Date: Mon, 1 Dec 2008 15:08:50 -0500 25, 25 -- Message-ID: {374357541C0244EF98A88684C030924C-at-Ken} 25, 25 -- MIME-Version: 1.0 25, 25 -- Content-Type: text/plain; 25, 25 -- charset="us-ascii" 25, 25 -- X-Priority: 3 (Normal) 25, 25 -- X-MSMail-Priority: Normal 25, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 25, 25 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 25, 25 -- In-Reply-To: {200812011915.mB1JF7X1022255-at-ns.microscopy.com} 25, 25 -- Importance: Normal 25, 25 -- Thread-Index: AclT6R3YNutimx5OSHCXSRfrsD5nlwAA6F4g 25, 25 -- Content-Transfer-Encoding: 8bit 25, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB1K8tq8002126 ==============================End of - Headers==============================
Active capture is like attaching an EDS system to the SEM and having its scan controller move the beam over the desired area. The SE detector signal output is digitized by the capture system as does an EDS imaging system. Normally, the SEM's scan generator drives the beam but in an active system, the separate capture unit drives the beam. The setup procedure must be done one time to calibrate max X and max Y voltages to get the captured aspect ratio to match that of the SEM. The next procedure is to set contrast and brightness in the capture system to match what is seen on TV display. The capture system will allow different x and y pixel dimensions as well as dwell time per pixel. This is like different slow scan speeds on the SEM but not always with allowed filtering amounts.
Passive uses the SEM scan generator to scan as normal. however, the capture system taps into the X signal, Y signal and usually the blanking signal. The active mode blanks the beam by itself to delete retrace lines. Both modes sync to power line to eliminate herringbone interference.
Bit resolution is determined by SEM scan speed in passive mode but by the user selection in active mode. The bit depth is hopefully at least 10 to 12 bits in either mode and is determined by hardware. In either event, the system needs calibration. If the A/D converter and buffer do say 4Kx4K pixels, the record CRT is for 4x5. So the x and y limits need to be adjusted in either mode one time.
Active is way more versatile than passive but also more expensive. Passive can also tap into main TV CRT if it has the NTSC RS-190 signals. Some makers do PAL as well as both modes.
gary g.
At 11:13 AM 12/1/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Mon Dec 1 14:33:40 2008 11, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB1KXeOe016008 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 14:33:40 -0600 11, 20 -- Message-Id: {200812012033.mB1KXeOe016008-at-ns.microscopy.com} 11, 20 -- Received: (qmail 24998 invoked from network); 1 Dec 2008 12:32:34 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 24994, pid: 24996, t: 0.1281s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp2 with SMTP; 1 Dec 2008 12:32:34 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Mon, 01 Dec 2008 12:33:37 -0800 11, 20 -- To: jkrupp-at-deltacollege.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Active vs passive image acquisition 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200812011913.mB1JDxkN020582-at-ns.microscopy.com} 11, 20 -- References: {200812011913.mB1JDxkN020582-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I can't say I have a protocol per se, but I think you should be able to examine the shale without equipment worries.
We purchased a VP-SEM years ago for use with concrete. We have used it with all manner of other materials including oily samples. I should probably point out that we do most of our work with BSE since an SE detector was not available for our Hitachi SEM. The situation might be different for the SE signal.
We do probably 90% of our work in VP-mode since we routinely encounter insulating samples. We use 40-100 Pa of helium as our residual gas to bleed away charge. The helium scatters much less than air or nitrogen at the same pressure. We often sweep the pressure over a range to determine the minimum pressure required to eliminate charging.
Since we are operating at a considerable pressure, we find that hydrocarbons are swept from the system. We have very little trouble with pump oil accumulating on the EDS window. By contrast, we need to clean the detector window on our other SEM (a conventional, high-vacuum scope) every 6 months or so as we see oil accumulating on the detector snout.
My biggest concern would be with the vacuum "pulling" the oil to the surface of the sample. We see such an effect with embedded and polished samples where polishing oil finds its way between the sample and embedding medium. The vacuum pulls it to the surface and the oil runs over the neighboring material. I suppose that could happen with your samples. Maybe a higher pressure would minimize the problem. Maybe you could find areas less affected.
Bottom line: I wouldn't hesitate to try it.
Warren Straszheim Iowa State University
-----Original Message----- X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net] Sent: Monday, December 01, 2008 9:04 AM To: wesaia-at-iastate.edu
I was wondering if any of the mineralogist on this list have developed a sample handling and SEM examination protocol for oil shale rock samples. I've only just received an inquery regarding the possibility, but I have never handled such a sample.
Our SEM is capable of environmental chamber pressures (FEI Quanta), but the work will require BEI imaging and EDX spectra. I imagine the vapor pressure from these types of samples can vary from nil to extremes - how would one determine before possibly contaminating the column if any particular sample was going to cause problems?
~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator INCO Innovation Centre Memorial University St. John's Newfoundland http://www.mun.ca/creait/maf/
==============================Original Headers============================== 4, 26 -- From michael-at-shaffer.net Mon Dec 1 09:02:40 2008 4, 26 -- Received: from smtprelay.hostedemail.com (smtprelay0055.hostedemail.com [216.40.44.55]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB1F2bfS016413 4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 09:02:40 -0600 4, 26 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 4, 26 -- by smtprelay01.hostedemail.com (Postfix) with SMTP id 6ED821BCE74E 4, 26 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 15:02:36 +0000 (UTC) 4, 26 -- X-SpamScore: 1 4, 26 -- X-Spam-Summary: 50,0,0,13c27218ee358141,0d355e4b7ffc0630,michael-at-shaffer.net,microscopy-at- microscopy.com,RULES_HIT:10:355:379:539:541:542:967:988:989:1155:1160:12 60:1277:1311:1313:1314:1345:1437:1515:1516:1518:1534:1539:1593:1594:1711 :1730:1747:1766:1792:2378:2393:2525:2560:2563:2682:2685:2693:2857:2859:2 933:2937:2939:2942:2945:2947:2951:2954:3022:3352:3636:3740:3865:3866:386 7:3868:3869:3871:3876:3877:3934:3936:3938:3941:3944:3947:3950:3953:3956: 3959:4250:5007:6114:6261:7679:8501:8526:8985:9010:9025:9388,0,RBL:none,C acheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 4, 26 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 4, 26 -- (Authenticated sender: michael-at-shaffer.net) 4, 26 -- by omf05.hostedemail.com (Postfix) with ESMTP 4, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 15:02:35 +0000 (UTC) 4, 26 -- From: "michael shaffer" {michael-at-shaffer.net} 4, 26 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 4, 26 -- Subject: SEM: oil shale sections 4, 26 -- Date: Mon, 1 Dec 2008 11:32:29 -0330 4, 26 -- Message-ID: {B117E0F5690B4EA8863434E19EB5CD00-at-CREAIT.MUN.CA} 4, 26 -- MIME-Version: 1.0 4, 26 -- Content-Type: text/plain; 4, 26 -- charset="us-ascii" 4, 26 -- Content-Transfer-Encoding: 7bit 4, 26 -- X-Mailer: Microsoft Office Outlook 11 4, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 4, 26 -- Thread-Index: AclTxdQu7FaJLHGoRC6lr19bLu8dRg== 4, 26 -- X-session-marker: 6D69636861656C40736861666665722E6E6574 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 36 -- From wesaia-at-iastate.edu Mon Dec 1 16:55:18 2008 17, 36 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 17, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB1MtHxx032624 17, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 16:55:17 -0600 17, 36 -- Received: from devirus-11.iastate.edu (devirus-11.iastate.edu [129.186.1.48]) 17, 36 -- by mailhub-5.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id mB1MtGP0028472; 17, 36 -- Mon, 1 Dec 2008 16:55:16 -0600 17, 36 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-11.iastate.edu with smtp 17, 36 -- id 1010_cc7de5c2_bffa_11dd_a184_001372578af6; 17, 36 -- Mon, 01 Dec 2008 16:52:57 -0600 17, 36 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 17, 36 -- by despam-10.iastate.edu (8.14.2/8.12.10) with ESMTP id mB1Mt9XY006049; 17, 36 -- Mon, 1 Dec 2008 16:55:09 -0600 17, 36 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 36 -- Mon, 1 Dec 2008 16:55:15 -0600 17, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 36 -- Content-class: urn:content-classes:message 17, 36 -- MIME-Version: 1.0 17, 36 -- Content-Type: text/plain; 17, 36 -- charset="us-ascii" 17, 36 -- Subject: RE: [Microscopy] SEM: oil shale sections 17, 36 -- Date: Mon, 1 Dec 2008 16:57:02 -0600 17, 36 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270371DE72-at-maire.eng.iastate.edu} 17, 36 -- In-Reply-To: {200812011503.mB1F3qLX017501-at-ns.microscopy.com} 17, 36 -- X-MS-Has-Attach: 17, 36 -- X-MS-TNEF-Correlator: 17, 36 -- Thread-Topic: [Microscopy] SEM: oil shale sections 17, 36 -- Thread-Index: AclTxgy2LAwoZvPlSn+3FlEn0OnyyAAQFSjA 17, 36 -- References: {200812011503.mB1F3qLX017501-at-ns.microscopy.com} 17, 36 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 17, 36 -- To: {michael-at-shaffer.net} , {Microscopy-at-microscopy.com} 17, 36 -- X-OriginalArrivalTime: 01 Dec 2008 22:55:15.0622 (UTC) FILETIME=[DFFDB460:01C95407] 17, 36 -- X-PMX-Version: 5.4.4.348488, Antispam-Engine: 2.6.0.325393, Antispam-Data: 2008.12.1.224329 17, 36 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_5000_5999 0, OEM_SOFTWARE_X1 0, __BOUNCE_CHALLENGE_SUBJ 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_5 0, __RUS_OBFU_PHONE 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' 17, 36 -- Content-Transfer-Encoding: 8bit 17, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB1MtHxx032624 ==============================End of - Headers==============================
I was just sifting through my listserv messages today and noticed your "little or no value" comment about Delta's program and was about to respond in a similar tone to Kleo, but then I also noticed your subsequent replies on the subject regarding your slightly out-of-date information, and I figured I would just set the record straight as far as the status of the program.
Judy Murphy (and probably others) did manage to secure the funding for the program, and it is now housed in its own specially designed building at Delta, the "Center for Microscopy and Allied Sciences." When I graduated from the program in June 2007, they had 3 TEM's, (one with STEM capability) 4 SEM's, (including a brand new Hitachi TM-1000, and a brand new JEOL) an AFM, a FIB, (which was not functional at the time, but may or may not be now) 3 darkrooms for film and paper processing and printing, a sectioning suite with at least 8 microtomes including an Ultracut E and one of the recent offerings from RMC, a new Cressington carbon evaporator and sputter coater, a Pelco lab microwave, two critical point driers, various new and old light microscopes for transmitted and reflected standard and fluorescence microscopy, a large image processing suite with all new computers, and a whole slew of materials-related equipment that I didn't really play around with much. I'm sure there's a lot more I'm forgetting, but that's the major stuff I can remember off the top of my head. This is also to say nothing of the blood, sweat, and tears that Cathy Davis, Frank Villalovos, (If I spelled that wrong, sorry Frank!) and the rest of the teaching staff put in to keep the place running and make sure we actually learned something at the same time.
Sorry for the massive run-on sentence, but hopefully this will clear up any ideas that the program at Delta is anything but going strong. The program primarily offers a 2 year certificate in either biological or materials science microscopy, or a combination of the two, and with a few more courses on top of that it is possible to obtain an Associate of Arts or Associate of Science degree.
Also, there is a bit about the program on the Delta College website at: http://www.deltacollege.edu/dept/electmicro/index.html
Hope this helps, -- Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
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Admittedly, I have old news. What Judy relayed to me on 16 Sept 2004 was that after her arduous work on securing funding for the EM building, the administration wanted to use it for social studies... or some unrelated field from EM. They were not interested in the low volume EM students. After her lengthy fight with the state for funding and finally securing it, a new administration began to tear her plan apart.
There are always two sides to a story. This is the one I got first hand from Judy. If things have changed for the better, please let me know and I will be the first to sing a new tune. A lot could have changed in four years.
gary g.
At 05:15 AM 11/25/2008, you wrote: } Gary, } I am interested in your comment,"It appears that the campus whackers } have made a very viable and valuable venue next to nothing of value...or } so it seems." } Please explain. } I look forward to your input as a student is interested in training at } that school. } Cheers, } Scott Streiker } Associate Research Electron Microscopist } University of Dayton Research Institute } Nanoscale Engineering Science and Technology (NEST) Laboratory } 937-229-5776 } www.nestlaboratory.com } } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Monday, November 24, 2008 12:37 AM } To: Streiker, Scott M. } Subject: [Microscopy] Re: viaWWW: academic programs in } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
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==============================Original Headers============================== 19, 27 -- From bnross-at-interchange.ubc.ca Mon Dec 1 18:48:56 2008 19, 27 -- Received: from mr3.mail-relay.ubc.ca (mr3.mail-relay.ubc.ca [137.82.45.5]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB20mu8J015938 19, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 18:48:56 -0600 19, 27 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 19, 27 -- by mr3.mail-relay.ubc.ca (Postfix) with ESMTP id 857FC1854B 19, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 16:48:53 -0800 (PST) 19, 27 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12]) 19, 27 -- by smtp.interchange.ubc.ca 19, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 19, 27 -- with ESMTP id {0KB800BQQ4XG3W-at-smtp.interchange.ubc.ca} for 19, 27 -- Microscopy-at-microscopy.com; Mon, 01 Dec 2008 16:48:53 -0800 (PST) 19, 27 -- Date: Mon, 01 Dec 2008 16:48:52 -0800 (PST) 19, 27 -- From: Bradford Ross {bnross-at-interchange.ubc.ca} 19, 27 -- Subject: Re: Academic programs in microscopy. 19, 27 -- To: Microscopy-at-microscopy.com 19, 27 -- Message-id: {16248382.15981228178932273.JavaMail.myubc2-at-brahms.my.ubc.ca} 19, 27 -- MIME-version: 1.0 19, 27 -- X-Mailer: uPortal WEB email client 3.0 19, 27 -- Content-type: text/plain; charset=us-ascii 19, 27 -- X-UBC-Scanned: Sophos PureMessage 5.4.6.353000, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.12.2.3121 19, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 19, 27 -- X-PerlMx-Spam: Probability=8%, Report=SUPERLONG_LINE 0.05, BODY_SIZE_7000_7999 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P2 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_NOT_1 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0 19, 27 -- X-Spam-Level: 19, 27 -- X-Spam-Flag: No 19, 27 -- Content-Transfer-Encoding: 8bit 19, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB20mu8J015938 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] eye glasses
Question: All,
It's time to get new eyeglass frames and lens so I'm writing for some advice on the frame size. My wife insists that I need to incorporate the "cool factor" (my choice of words) in this purchase. The "cool" frames are vertically narrow (25 to 30 mm) and the optometrist says it is difficult to fit progressive lens in this dimension. Like many of you, I do a lot of close-up and computer work. I do not have trouble seeing distances over 6 feet. The doctor suggested it may be wise to have 2 sets of glasses, one for work and the other when I need to make a fashion statement. How have you all addresses this issue? Do the narrow lens work for microscopy? Do you have 2 pair? Other ideas or comments. Thanks in advance for your responses.
I've always said "Bah on making a fashion statement!!". I get the best available safety frames (with safety lenses), which accommodate my tri-focal lenses comfortably and give maximum protection to my eyes. I've had my eyesight saved three times by my glasses, and want the best protection and most serviceable system I can get. Safety frames are usually a lot cheaper than the fashionable ones, too.
Wil Bigelow ===================================
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-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 9, 16 -- From bigelow-at-umich.edu Mon Dec 1 20:25:52 2008 9, 16 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB22PqNA012039 9, 16 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 20:25:52 -0600 9, 16 -- Received: FROM [76.232.133.196] (adsl-76-232-133-196.dsl.sfldmi.sbcglobal.net [76.232.133.196]) 9, 16 -- BY skycaptain.mr.itd.umich.edu ID 49349CAF.5B419.28179 ; 9, 16 -- 1 Dec 2008 21:25:51 -0500 9, 16 -- Mime-Version: 1.0 9, 16 -- Message-Id: {p06240800c55a4b6e6ca8-at-[76.234.167.108]} 9, 16 -- In-Reply-To: {200812020147.mB21lfgL007018-at-ns.microscopy.com} 9, 16 -- References: {200812020147.mB21lfgL007018-at-ns.microscopy.com} 9, 16 -- Date: Mon, 1 Dec 2008 21:25:47 -0500 9, 16 -- To: opmills-at-mtu.edu, Microscopy Listserver {microscopy-at-microscopy.com} 9, 16 -- From: Wil Bigelow {bigelow-at-umich.edu} 9, 16 -- Subject: Re: [Microscopy] viaWWW: eye glasses 9, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I managed to get very fashionable vertically narrow glasses (with lightning bolts, no less) with progressive lenses that work for me. The trick was to take an actual box of grids and forceps to the eye doctor, as well as a variety of other items I routinely work with. Then they were able to do all that flipping of lenses manually without the massive thing that hangs in front of your face, so I could look down at the grids and then up to a computer monitor at head angles that were more like real life. Now I have a geat pair of glasses! This made all the difference in the world, and I recommend it.
I do take them off to look through microscope binoculars.
Aloha, Tina
} Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] eye glasses } } Question: All, } } It's time to get new eyeglass frames and lens so I'm writing for some } advice on the frame size. My wife insists that I need to incorporate } the "cool factor" (my choice of words) in this purchase. The "cool" } frames are vertically narrow (25 to 30 mm) and the optometrist says } it is difficult to fit progressive lens in this dimension. Like many } of you, I do a lot of close-up and computer work. I do not have } trouble seeing distances over 6 feet. The doctor suggested it may be } wise to have 2 sets of glasses, one for work and the other when I } need to make a fashion statement. How have you all addresses this } issue? Do the narrow lens work for microscopy? Do you have 2 pair? } Other ideas or comments. Thanks in advance for your responses.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Mon Dec 1 21:22:42 2008 7, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB23Mf6R026845 7, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 21:22:42 -0600 7, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id mB23MbdB028184 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 21 -- Mon, 1 Dec 2008 17:22:38 -1000 (HST) 7, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id mB23MaIU028181; 7, 21 -- Mon, 1 Dec 2008 17:22:37 -1000 (HST) 7, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Mon, 1 Dec 2008 17:22:36 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-Sender: tina-at-halia 7, 21 -- To: opmills-at-mtu.edu 7, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] viaWWW: eye glasses 7, 21 -- In-Reply-To: {200812020144.mB21i87g031533-at-ns.microscopy.com} 7, 21 -- Message-ID: {Pine.GSO.4.21.0812011716390.28035-100000-at-halia} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I use narrow frameless progressive lenses for up close and distance. I don't peer through a microscope, but spend a lot of time in front of a computer screen. They work fine for that, but there is a 'band' in the lens where the computer screen is in focus, so there's some adjustment to be made. I tried using several pair of different glasses but that was really a hassle, so I advise against that. Oh yea, I think they're 'cool'! I'm new at posting messages, so I hope you get this.
Don Kloos VP Sales, Marketing, Business Development
-----Original Message----- X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu] Sent: Monday, December 01, 2008 5:49 PM To: dkloos-at-parallaxray.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] eye glasses
Question: All,
It's time to get new eyeglass frames and lens so I'm writing for some advice on the frame size. My wife insists that I need to incorporate the "cool factor" (my choice of words) in this purchase. The "cool" frames are vertically narrow (25 to 30 mm) and the optometrist says it is difficult to fit progressive lens in this dimension. Like many of you, I do a lot of close-up and computer work. I do not have trouble seeing distances over 6 feet. The doctor suggested it may be wise to have 2 sets of glasses, one for work and the other when I need to make a fashion statement. How have you all addresses this issue? Do the narrow lens work for microscopy? Do you have 2 pair? Other ideas or comments. Thanks in advance for your responses.
Owen: I am very near-sighted but like many of us in a certain age bracket, I need bifocals to read comfortably. My current pair of frames are fashionably narrow and I don't like them much. The bottom frame is right in my field of view looking down to read and it annoys me. I have no problems with the optical scopes, the SEMs, or my computer monitor. My safety glasses for lab use have the old-style bifocals and they are a pain to use in the optical scopes as the top line of the bifocal lens is right in the middle of my field of view. My company pays for my safety glasses, but progressive lenses for them is a slight out-of-pocket expense and I didn't think it would be a big deal. Was I wrong!
opmills-at-mtu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] eye glasses } } Question: All, } } It's time to get new eyeglass frames and lens so I'm writing for some } advice on the frame size. My wife insists that I need to incorporate } the "cool factor" (my choice of words) in this purchase. The "cool" } frames are vertically narrow (25 to 30 mm) and the optometrist says } it is difficult to fit progressive lens in this dimension. Like many } of you, I do a lot of close-up and computer work. I do not have } trouble seeing distances over 6 feet. The doctor suggested it may be } wise to have 2 sets of glasses, one for work and the other when I } need to make a fashion statement. How have you all addresses this } issue? Do the narrow lens work for microscopy? Do you have 2 pair? } Other ideas or comments. Thanks in advance for your responses. } } Regards, } } Owen Mills } } } } Login Host: 141.219.192.45 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Mon Dec 1 19:43:17 2008 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB21hGCe030060 } 11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 19:43:17 -0600 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240802c55a4327222d-at-[206.69.208.22]} } 11, 11 -- Date: Mon, 1 Dec 2008 19:43:15 -0600 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: eye glasses } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } }
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==============================Original Headers============================== 4, 22 -- From r-holdford-at-ti.com Mon Dec 1 23:52:47 2008 4, 22 -- Received: from comal.ext.ti.com (comal.ext.ti.com [198.47.26.152]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB25qleh024268 4, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 23:52:47 -0600 4, 22 -- Received: from dlep34.itg.ti.com ([157.170.170.115]) 4, 22 -- by comal.ext.ti.com (8.13.7/8.13.7) with ESMTP id mB25qgVG027947; 4, 22 -- Mon, 1 Dec 2008 23:52:47 -0600 4, 22 -- Received: from [156.117.248.174] (localhost [127.0.0.1]) 4, 22 -- by dlep34.itg.ti.com (8.13.7/8.13.7) with ESMTP id mB25qfx7013570; 4, 22 -- Mon, 1 Dec 2008 23:52:42 -0600 (CST) 4, 22 -- Message-ID: {4934CD29.4090701-at-ti.com} 4, 22 -- Date: Mon, 01 Dec 2008 23:52:41 -0600 4, 22 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 22 -- Organization: SC Packaging Development -- FA Development 4, 22 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 4, 22 -- MIME-Version: 1.0 4, 22 -- To: opmills-at-mtu.edu, MSA Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] viaWWW: eye glasses 4, 22 -- References: {200812020143.mB21hRi1030314-at-ns.microscopy.com} 4, 22 -- In-Reply-To: {200812020143.mB21hRi1030314-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
} } FOCUS ON MICROSCOPY 2009 - Krakow, Poland, 5-8 April 2009 } } 22nd International Conference on 3D Image Processing in Microscopy } 21st International Conference on Confocal Microscopy } } Dear Colleagues, } } Abstracts for oral and poster contributions can now be submitted } preferably through the conference website: } http://www.FocusOnMicroscopy.org where also the conference registration } has opened and hotel information is available. Deadline for abstract } submission: January 19, 2009. } } After the successful FOM2008 conference held in Osaka in April this year, } the next conference: Focus on Microscopy 2009 will take place in Krakow, } Poland, in the week before Easter from Sunday April 5 to Wednesday April } 8, 2009. } } As the next in a series of unique interdisciplinary meetings on advanced } multi-dimensional light microscopy and image processing, the conference } will be hosted by Jagiellonian University in Krakow at the Auditorium } Maximum building in the center of this historic and beautiful city. } } Focus on Microscopy 2009 is the continuation of a yearly conference series } presenting the latest innovations and new trends in optical microscopy and } their application in biology, medicine and material sciences. } } Key subjects are: } } * the theory and practice of multi-dimensional optical microscopes, } * the high spatial resolution and sophisticated excitation (SHG, Raman, } THG, OCS) and fluorescence imaging modes (FRET,FLIM, FRAP, FCS) coming } available, } * new techniques for functional studies and manipulation of cells and } tissues, } * automated and high-throughput microscopy techniques, } * related image processing and analysis approaches. } } The conference series is, in addition, known for covering the rapid } development of advanced fluorescence labeling techniques for the confocal } and multi-photon 3D imaging of -live- biological specimens. Laser light } based activation and quenching techniques integrated with 3D microscopy } are becoming important tools in cell biology. } } Important dates: } } Deadline for the submission of abstracts: January 19, 2009 } Acceptance of contributions, draft program: February 10, 2009 } Deadline for early registration: February 28, 2009 } } We welcome you to Krakow for the FOM2009 conference and exhibition. To } stay informed about the conference please leave your name and email at: } http://www.FocusOnMicroscopy.org/stayinformed } } On behalf of the organizing committee, } } - Jurek Dobrucki, Jagiellonian University, Krakow, Poland } - Fred Brakenhoff, University of Amsterdam, The Netherlands } -- } E-mail: info2009-at-FocusOnMicroscopy.org } Web: www.FocusOnMicroscopy.org
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==============================Original Headers============================== 4, 26 -- From hubner-at-iod.krakow.pl Tue Dec 2 02:10:31 2008 4, 26 -- Received: from sowa.iod.krakow.pl (sowa.IOd.krakow.pl [149.156.29.201]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB28ATtD008265 4, 26 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 02:10:30 -0600 4, 26 -- Received: from SERWER_SQL (serwer_sql [149.156.29.202]) 4, 26 -- by sowa.iod.krakow.pl (8.13.6+Sun/8.13.6) with SMTP id mB27nttS013029 4, 26 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 08:49:55 +0100 (CET) 4, 26 -- Received: from [149.156.29.4] (helo=iodkh007) 4, 26 -- by SERWER_SQL with AVK MailGateway; 4, 26 -- for {microscopy-at-microscopy.com} ; Tue, 02 Dec 2008 09:11:01 +0100 4, 26 -- Date: Tue, 2 Dec 2008 09:10:34 +0100 4, 26 -- From: =?iso-8859-2?Q?Krzysztof_H=FCbner?= {hubner-at-iod.krakow.pl} 4, 26 -- To: "Microscopy list" {microscopy-at-microscopy.com} 4, 26 -- MIME-Version: 1.0 4, 26 -- Message-ID: {2624FBC472CE49478481A6B361B933F9-at-iodkh007} 4, 26 -- Subject: FOM2009, Abstracts, Registration, Krakow, Poland, 5-8 April 2009 4, 26 -- Content-Type: text/plain; 4, 26 -- charset="iso-8859-2"; 4, 26 -- format="flowed"; 4, 26 -- reply-type="original" 4, 26 -- Content-Transfer-Encoding: 7bit 4, 26 -- X-Priority: 3 4, 26 -- X-MSMail-Priority: Normal 4, 26 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5512 4, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 4, 26 -- X-AVK-Virus-Check: AVF 19.169;01.12.2008 ==============================End of - Headers==============================
Speaking as someone who is optically challenged (hey, my lenses are bullet-proof), I'd go for the 2 sets, but not necessarily near & far. If. First, do you have stigmatism? Second, do the binocs on the (S)TEMs have sufficient diopter adjustment to compensate for your prescription? If "no" and "yes", respectively, quit wearing your glasses at the TEM! They get in the way. If not: Are the oculars on the binocs high eye-point? Or normal? They should have a little eyeglasses icon on the oculars if they are high eye-point. If they're normal, then your eyes (retinas) are too far from the ocular focal plane for the best focus, etc., when you're wearing glasses. If you must wear glasses at the 'scope, get high eye-point oculars.
Have you considered contacts for 'scope work? These can be the best solution, if you don't have stigmatism. There are some contacts that can correct mild or weak stigmatism, but not necessarily. Not mine, for instance. Tried contacts, almost worked, but too much stigmatism.
Second, if you have to wear glasses, don't bother with both near-vision and far-vision. Get the one prescription you need for non-'scope work (sounds like near vision and why bother with anything else, if you don't need it?). The binocs on the scope will have enough adjustment to make up the difference. If you get high eye-point oculars. Get 2 pairs because the oculars will scratch the lenses. Might as well have one pair for circular ocular-scratches and one pair for society balls.
The other secret: get small-lens frames (less curvature, thinner lenses), and get safety-frames, say from (sorry) WalMart. Save about $100 or more doing that. Safety frames are as fashionable -- a big deal in Houghton, I know -- and both sturdier and MUCH cheaper than "normal" frames. Not to mention "cool" frames. And the U. may pay for them, since they're safety glasses. Besides, you don't shop for something based on the "cool factor". Remember the final test novice test of the Brothers of Cool. (When shown an wardrobe full of clothes and asked which are the coolest to wear, the correct answer is "Whatever I put on.")
Phil
} Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] eye glasses } } Question: All, } } It's time to get new eyeglass frames and lens so I'm writing for some } advice on the frame size. My wife insists that I need to incorporate } the "cool factor" (my choice of words) in this purchase. The "cool" } frames are vertically narrow (25 to 30 mm) and the optometrist says } it is difficult to fit progressive lens in this dimension. Like many } of you, I do a lot of close-up and computer work. I do not have } trouble seeing distances over 6 feet. The doctor suggested it may be } wise to have 2 sets of glasses, one for work and the other when I } need to make a fashion statement. How have you all addresses this } issue? Do the narrow lens work for microscopy? Do you have 2 pair? } Other ideas or comments. Thanks in advance for your responses. } } Regards, } } Owen Mills -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 26 -- From oshel1pe-at-cmich.edu Tue Dec 2 07:54:12 2008 7, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2DsCgJ030632 7, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 07:54:12 -0600 7, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 26 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mB2Ds4UG022442; 7, 26 -- Tue, 2 Dec 2008 08:54:11 -0500 7, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 7, 26 -- Tue, 2 Dec 2008 08:53:23 -0500 7, 26 -- Mime-Version: 1.0 7, 26 -- Message-Id: {f06240803c55ae83405be-at-[141.209.160.249]} 7, 26 -- In-Reply-To: {200812020146.mB21kIo3003705-at-ns.microscopy.com} 7, 26 -- References: {200812020146.mB21kIo3003705-at-ns.microscopy.com} 7, 26 -- Date: Tue, 2 Dec 2008 08:53:20 -0500 7, 26 -- To: opmills-at-mtu.edu 7, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 26 -- Subject: Re: [Microscopy] viaWWW: eye glasses 7, 26 -- Cc: Microscopy-at-microscopy.com 7, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 26 -- X-OriginalArrivalTime: 02 Dec 2008 13:53:23.0449 (UTC) FILETIME=[57A38E90:01C95485] 7, 26 -- X-Canit-CHI2: 0.00 7, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 26 -- X-Spam-Score: -4.10 () [Hold at 5.00] L_EXCH_MF,L_USD,L_USDD,RDNS_NONE,Bayes(0.0001,-0.5) 7, 26 -- X-CanItPRO-Stream: default 7, 26 -- X-Canit-Stats-ID: 6021855 - 7421bfa1a0b3 7, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Hi Owen- I couple of years ago, I too went for the "cool" factor and picked out a really great pair of designer frames that were pretty narrow rectangles. The opticians (a big, national chain) assured me that they could fit my full progressive Rx into the frame.. they did, but the transitions from were tight, and I almost killed myself the first time I tried to walk down a staircase and glanced down (and below) the frames and couldn't see where I was going. Luckily, this particular chain of stores has a 30-day trial period for all frames and I was able to go back, pick a pair of less-cool, but more functional frames. I wear multi-focal contact lenses in the lab, since I've always had a hard time forming a merged image when looking through the binocs with glasses on. If you can wear progressive-lens eyeglasses, and can tolerate contacts, you can most likely use the multi-focal contacts with success. I love 'em. Lee
} } Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] eye glasses } } Question: All, } } It's time to get new eyeglass frames and lens so I'm writing for some } advice on the frame size. My wife insists that I need to incorporate } the "cool factor" (my choice of words) in this purchase. The "cool" } frames are vertically narrow (25 to 30 mm) and the optometrist says } it is difficult to fit progressive lens in this dimension. Like many } of you, I do a lot of close-up and computer work. I do not have } trouble seeing distances over 6 feet. The doctor suggested it may be } wise to have 2 sets of glasses, one for work and the other when I } need to make a fashion statement. How have you all addresses this } issue? Do the narrow lens work for microscopy? Do you have 2 pair? } Other ideas or comments. Thanks in advance for your responses. } } Regards, } } Owen Mills } } } } Login Host: 141.219.192.45 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 11 -- From zaluzec-at-microscopy.com Mon Dec 1 19:43:17 2008 } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id mB21hGCe030060 } 11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 19:43:17 -0600 } 11, 11 -- Mime-Version: 1.0 } 11, 11 -- Message-Id: {p06240802c55a4327222d-at-[206.69.208.22]} } 11, 11 -- Date: Mon, 1 Dec 2008 19:43:15 -0600 } 11, 11 -- To: microscopy-at-microscopy.com } 11, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver) } 11, 11 -- Subject: viaWWW: eye glasses } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
-- Lee Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
As a 50-something, I've found that anything to do with vision is a compromise. When using the TEM with binoculars, I had developed a rapid glasses on-glasses off routine that would have made the Karate Kid proud. When using a computer, I either parked myself half a block away from the screen and perched my single visions on the end of my nose OR I used bifocals and got my neck adjusted by a chiropractor as needed. Considered paying him a retainer for unlimited walk (crawl?) ins.
Finally I discovered multifocal contacts. Advantages: I can read near and far, I can use them with binoculars, and I can walk down steps and along a rough path without doing the bifocal boogie (i.e., Stumble! What was That!). Disadvantages: the usual occasional discomfort, expense (insurance may not keep up with demand), vision is not perfect at any distance (but good enough, generally), and ultra-close vision still requires a boost like reading glasses.
Like I said, a compromise, but so far the one that seems to work best for me, at least until they perfect whole body transplants or reverse the aging process.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com/
==============================Original Headers============================== 7, 27 -- From TindallR-at-missouri.edu Tue Dec 2 08:46:48 2008 7, 27 -- Received: from mxtip01-missouri-out.um.umsystem.edu (mxtip01-missouri-out.um.umsystem.edu [209.106.229.45]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2EkmIE026424 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 08:46:48 -0600 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: ApoEALvYNEnRauUp/2dsb2JhbADHFAEJhmmEfYF/gQA 7, 27 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 7, 27 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 02 Dec 2008 08:46:47 -0600 7, 27 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 7, 27 -- Tue, 2 Dec 2008 08:46:46 -0600 7, 27 -- x-mimeole: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- Subject: Eyeglasses 7, 27 -- Date: Tue, 2 Dec 2008 08:46:46 -0600 7, 27 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7C14-at-UM-XMAIL08.um.umsystem.edu} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Eyeglasses 7, 27 -- Thread-Index: AclUjMyINdcp1CB9RQK6tE4pnS8KFA== 7, 27 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 7, 27 -- To: {microscopy-at-microscopy.com} 7, 27 -- X-OriginalArrivalTime: 02 Dec 2008 14:46:46.0546 (UTC) FILETIME=[CCD55F20:01C9548C] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2EkmIE026424 ==============================End of - Headers==============================
I was a safety warden for about 18 years. In our lab(s) safety glasses were required. No contact lenses were allowed because they trapped chemicals behind the contacts. So, all that meant that only Z-87 safety frames were allowed. They had to be a minimum of 3 mm thick and could be either glass or plastic. Every covered pair was checked by the safety department for the Z-87 stamping on the side rails of the frames and the thickness over the entire lens.
I always used glass eyewear and not plastic. Progressive or "blended bifocals" help a lot in microscopy. It takes a new wearer at least three weeks of complaints until they get used to the out of focus areas. After three weeks, you are home free for the rest of your life unless the fitting was not done right. The "cylinder" area must be located and centered correctly. Use a known competent supplier that others recommend. A safety inspector asked me why I was the only one in the microscopy department that didn't take their glasses off when looking into a microscope (on a RMC microtome). This became a BIG safety issue with some inspectors. I relied, "I changed the eyepiece focal length so I wouldn't have to remove them." Bumping the eyepiece with glass is no problem as far as scratches.
As for being cool, you won't look very cool without one of your eyes, maybe even sporting a red and white cane, or a black eye patch. I had molten silver metal strike my glasses and later a piece of concrete that was hit with a hammer. The concrete "starred" the one lens and it had to be replaced.
Here's a bit of perspective on objects hitting lenses and some marketing gimmicks from both sides. Glass lenses will not break in a standard *weighted* ball drop test but they do in an equivalent dart drop test. It should be noted that the lenses were placed on an immovable mount, unlike real life frames that give during a large object impact. Plastic breaks with a ball drop test but not a dart drop test. So plastic would seem to be the better choice because impact objects are not round balls. This marketing scheme ignores the energy absorption of the frames in either case. Plastic still is not for me.
I asked a respected lenses researcher and tester about the scratch resistance and hardness issues. He said, quote, "If plastic has a hardness rating of one, then on that scale glass is 5000. Hardcoats might get the number up to two or three." I always bought glass after that.
I watched one day as a plastic lens fell out my bosses (thinner) wire rim fashion Z-87 frames when walking out into winter weather. Z-87 frames have a deeper groove to hold the lens in place in an impact. However, plastic lenses shrink more when cold and when they are in thinner grooved wire rim frames. Now switch your thinking to thicker plastic frames and glass lenses.
This should give you something to think about from a safety perspective. I still think glass has the edge overall.
Disclaimer: My lab produced CR-39® resin and I watched many coworkers test plastic CR-39® lenses and glass lenses NOT mounted in frames. I never saw plastic or glass lenses break like those in those drop tests on anyone's lab nose but it probably has happened with a high velocity and mass impact.
No rights reserved.
JMO,
Paul
At 08:26 PM 12/1/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Most large companies, government labs and schools usually offer free or discount prescription safety glasses to employees.
I have regular bifocals and a set of computer/reading glasses for normal life, and two sets of safety glasses for work. My son noticed that over a weekend recently that my glasses were larger than normal-- I forgot to change before going home. The choice in safety frames can get you around in a pinch without too much family embarrassment.
Check out: http://www.admin.mtu.edu/urel/ttoday/previous.php?issue=20080923#3 for your local discounts.
Cheers, Roseann
On Dec 1, 2008, at 5:48 PM, opmills-at-mtu.edu wrote:
} } } } Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] eye glasses } } Question: All, } } It's time to get new eyeglass frames and lens so I'm writing for some } advice on the frame size. My wife insists that I need to incorporate } the "cool factor" (my choice of words) in this purchase. The "cool" } frames are vertically narrow (25 to 30 mm) and the optometrist says } it is difficult to fit progressive lens in this dimension. Like many } of you, I do a lot of close-up and computer work. I do not have } trouble seeing distances over 6 feet. The doctor suggested it may be } wise to have 2 sets of glasses, one for work and the other when I } need to make a fashion statement. How have you all addresses this } issue? Do the narrow lens work for microscopy? Do you have 2 pair? } Other ideas or comments. Thanks in advance for your responses. } } Regards, } } Owen Mills
==============================Original Headers============================== 13, 26 -- From RCsencsits-at-lbl.gov Tue Dec 2 10:48:02 2008 13, 26 -- Received: from ironport2.lbl.gov (ironport2.lbl.gov [128.3.41.14]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2Gm1Qp024085 13, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 10:48:02 -0600 13, 26 -- X-Ironport-SBRS: 2.3 13, 26 -- X-IronPort-Anti-Spam-Filtered: true 13, 26 -- X-IronPort-Anti-Spam-Result: AhoCAAf2NEmAAykMe2dsb2JhbACTTQEBFiIFvliCf4Nl 13, 26 -- X-IronPort-AV: E=Sophos;i="4.33,702,1220252400"; 13, 26 -- d="scan'208";a="94034822" 13, 26 -- Received: from mta2.lbl.gov ([128.3.41.12]) 13, 26 -- by ironport2.lbl.gov with ESMTP; 02 Dec 2008 08:48:01 -0800 13, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) 13, 26 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id mB2Gm0nv004731; 13, 26 -- Tue, 2 Dec 2008 08:48:00 -0800 (PST) 13, 26 -- Cc: Microscopy-at-microscopy.com 13, 26 -- Message-Id: {18F5D5DD-C31E-41F8-A4EF-2606621B6DC9-at-lbl.gov} 13, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 13, 26 -- To: opmills-at-mtu.edu 13, 26 -- In-Reply-To: {200812020148.mB21mBIT008378-at-ns.microscopy.com} 13, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 26 -- Content-Transfer-Encoding: 7bit 13, 26 -- Mime-Version: 1.0 (Apple Message framework v929.2) 13, 26 -- Subject: Re: [Microscopy] viaWWW: eye glasses 13, 26 -- Date: Tue, 2 Dec 2008 08:47:53 -0800 13, 26 -- References: {200812020148.mB21mBIT008378-at-ns.microscopy.com} 13, 26 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
We are currently renegotiating our service contract with the manufacturers of our SEMs. In order to ensure a fair appraisal of the proposal (we work for government), I need to find out if there are alternatives other than the original manufacturer.
Are you aware of any third party company specialized in SEM maintenance that would service two SEMs in Edmonton, Alberta, Canada?
Thank you for your help, Daniel
__________________________________________ Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC 11421 Saskatchewan Dr. Edmonton, AB. T6G 2M9
Phone: (780) 641-1663 Fax: (780) 641-1601
==============================Original Headers============================== 9, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Tue Dec 2 11:05:58 2008 9, 23 -- Received: from nrccenexf1.nrc.ca (nrccenexf1.nrc.ca [132.246.15.80]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2H5woi005502 9, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Dec 2008 11:05:58 -0600 9, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf1.nrc.ca with Microsoft SMTPSVC(6.0.3790.3959); 9, 23 -- Tue, 2 Dec 2008 12:05:57 -0500 9, 23 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: SEM third party maintenance 9, 23 -- Date: Tue, 2 Dec 2008 12:05:56 -0500 9, 23 -- Message-ID: {6AF37B759D23CD4FB862A21C1F06CF97613BD9-at-nrccenexb2.nrc.ca} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: SEM third party maintenance 9, 23 -- Thread-Index: AclUoD3jH6ZkONG7SlKC+l1nrFJ7PA== 9, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca} 9, 23 -- To: {Microscopy-at-Microscopy.Com} 9, 23 -- X-OriginalArrivalTime: 02 Dec 2008 17:05:57.0910 (UTC) FILETIME=[3EA26360:01C954A0] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2H5woi005502 ==============================End of - Headers==============================
} } Glass is cool.... } } But some of us have Rx's so strong glass is not feasible! } } I had a pair of glass glasses that the frame broke off my face, } exactly at the point I picked up a Toxilab jar full of concentrated } sulfuric aid ;-) } I've had to use plastic ever since. But I've had a lot less sinus } issues from the weight of the glasses. } } But I agree with the glasses saving one's eyes, and tend to buy the } bigger lenses just for that reason. I do tend to have a lot of } issues changing glasses of even the same Rx, so do tend to stick to } one pair. } } } } Lou Ann } } } } } On Dec 2, 2008, at 10:19 AM, beaurega-at-westol.com wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Owen, } } } } I was a safety warden for about 18 years. In our lab(s) safety } } glasses } } were required. No contact lenses were allowed because they trapped } } chemicals behind the contacts. So, all that meant that only Z-87 } } safety } } frames were allowed. They had to be a minimum of 3 mm thick and } } could be } } either glass or plastic. Every covered pair was checked by the } } safety } } department for the Z-87 stamping on the side rails of the frames } } and the } } thickness over the entire lens. } } } } I always used glass eyewear and not plastic. Progressive or "blended } } bifocals" help a lot in microscopy. It takes a new wearer at least } } three } } weeks of complaints until they get used to the out of focus areas. } } After } } three weeks, you are home free for the rest of your life unless the } } fitting } } was not done right. The "cylinder" area must be located and centered } } correctly. Use a known competent supplier that others recommend. } } A safety } } inspector asked me why I was the only one in the microscopy } } department that } } didn't take their glasses off when looking into a microscope (on a } } RMC } } microtome). This became a BIG safety issue with some inspectors. I } } relied, "I changed the eyepiece focal length so I wouldn't have to } } remove } } them." Bumping the eyepiece with glass is no problem as far as } } scratches. } } } } As for being cool, you won't look very cool without one of your } } eyes, maybe } } even sporting a red and white cane, or a black eye patch. I had } } molten } } silver metal strike my glasses and later a piece of concrete that } } was hit } } with a hammer. The concrete "starred" the one lens and it had to } } be replaced. } } } } Here's a bit of perspective on objects hitting lenses and some } } marketing } } gimmicks from both sides. Glass lenses will not break in a standard } } *weighted* ball drop test but they do in an equivalent dart drop } } test. It } } should be noted that the lenses were placed on an immovable mount, } } unlike } } real life frames that give during a large object impact. Plastic } } breaks } } with a ball drop test but not a dart drop test. So plastic would } } seem to } } be the better choice because impact objects are not round balls. } } This } } marketing scheme ignores the energy absorption of the frames in } } either } } case. Plastic still is not for me. } } } } I asked a respected lenses researcher and tester about the scratch } } resistance and hardness issues. He said, quote, "If plastic has a } } hardness } } rating of one, then on that scale glass is 5000. Hardcoats might } } get the } } number up to two or three." I always bought glass after that. } } } } I watched one day as a plastic lens fell out my bosses (thinner) } } wire rim } } fashion Z-87 frames when walking out into winter weather. Z-87 } } frames have } } a deeper groove to hold the lens in place in an impact. However, } } plastic } } lenses shrink more when cold and when they are in thinner grooved } } wire rim } } frames. Now switch your thinking to thicker plastic frames and } } glass lenses. } } } } This should give you something to think about from a safety } } perspective. I } } still think glass has the edge overall. } } } } Disclaimer: My lab produced CR-39® resin and I watched many } } coworkers test } } plastic CR-39® lenses and glass lenses NOT mounted in frames. I } } never saw } } plastic or glass lenses break like those in those drop tests on } } anyone's } } lab nose but it probably has happened with a high velocity and mass } } impact. } } } } No rights reserved. } } } } JMO, } } } } Paul } } } } At 08:26 PM 12/1/08 -0600, you wrote: } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Owen: } } } } } } I've always said "Bah on making a fashion statement!!". I get the } } } best available safety frames (with safety lenses), which accommodate } } } my tri-focal lenses comfortably and give maximum protection to my } } } eyes. I've had my eyesight saved three times by my glasses, and } } } want } } } the best protection and most serviceable system I can get. Safety } } } frames are usually a lot cheaper than the fashionable ones, too. } } } } } } Wil Bigelow } } } =================================== } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } } of America } } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/ } } } } FAQ.html } } } } ---------------------------------------------------------------------------- } } } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } } using the WWW based Form at } } } } http://www.microscopy.org/MicroscopyListserver/MLFormMail.html } } } } --------------------------------------------------------------------------- } } } } Remember this posting is most likely not from a Subscriber, so } } } } when replying } } } } please copy both opmills-at-mtu.edu as well as the MIcroscopy } } } } Listserver } } } } --------------------------------------------------------------------------- } } } } } } } } Email: opmills-at-mtu.edu } } } } Name: Owen Mills } } } } } } } } Organization: Michigan Tech University } } } } } } } } Title-Subject: [Filtered] eye glasses } } } } } } } } Question: All, } } } } } } } } It's time to get new eyeglass frames and lens so I'm writing for } } } } some } } } } advice on the frame size. My wife insists that I need to } } } } incorporate } } } } the "cool factor" (my choice of words) in this purchase. The } } } } "cool" } } } } frames are vertically narrow (25 to 30 mm) and the optometrist says } } } } it is difficult to fit progressive lens in this dimension. Like } } } } many } } } } of you, I do a lot of close-up and computer work. I do not have } } } } trouble seeing distances over 6 feet. The doctor suggested it } } } } may be } } } } wise to have 2 sets of glasses, one for work and the other when I } } } } need to make a fashion statement. How have you all addresses this } } } } issue? Do the narrow lens work for microscopy? Do you have 2 pair? } } } } Other ideas or comments. Thanks in advance for your responses. } } } } } } } } Regards, } } } } } } } } Owen Mills } } } } } } } } } } } } } } } } Login Host: 141.219.192.45 } } } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original } } } } Headers============================== } } } } 11, 11 -- From zaluzec-at-microscopy.com Mon Dec 1 19:43:17 2008 } } } } 11, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } } } [206.69.208.22]) } } } } 11, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } } ESMTP id mB21hGCe030060 } } } } 11, 11 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 } } } } 19:43:17 -0600 } } } } 11, 11 -- Mime-Version: 1.0 } } } } 11, 11 -- Message-Id: {p06240802c55a4327222d-at-[206.69.208.22]} } } } } 11, 11 -- Date: Mon, 1 Dec 2008 19:43:15 -0600 } } } } 11, 11 -- To: microscopy-at-microscopy.com } } } } 11, 11 -- From: opmills-at-mtu.edu (by way of MicroscopyListserver) } } } } 11, 11 -- Subject: viaWWW: eye glasses } } } } 11, 11 -- Content-Type: text/plain; charset="us-ascii" ; } } } } format="flowed" } } } } ==============================End of - } } } } Headers============================== } } } } } } } } } -- } } } Wilbur C. Bigelow, Professor Emeritus } } } Materials Sci. & Engr., Univ. of Michigan } } } Ann Arbor, Michigan 48109-2136 } } } e-mail: bigelow-at-umich.edu; } } } Fx:734-763-4788; Ph:734-975-0858 } } } Address mail to: 2911 Whittier Court } } } Ann Arbor, MI 48104-6731 } } } } } } ==============================Original } } } Headers============================== } } } 9, 16 -- From bigelow-at-umich.edu Mon Dec 1 20:25:52 2008 } } } 9, 16 -- Received: from skycaptain.mr.itd.umich.edu } } } (smtp.mail.umich.edu } } [141.211.93.160]) } } } 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } } ESMTP id } } mB22PqNA012039 } } } 9, 16 -- for {microscopy-at-microscopy.com} ; Mon, 1 Dec 2008 } } } 20:25:52 -0600 } } } 9, 16 -- Received: FROM [76.232.133.196] } } (adsl-76-232-133-196.dsl.sfldmi.sbcglobal.net [76.232.133.196]) } } } 9, 16 -- BY skycaptain.mr.itd.umich.edu ID 49349CAF.5B419.28179 ; } } } 9, 16 -- 1 Dec 2008 21:25:51 -0500 } } } 9, 16 -- Mime-Version: 1.0 } } } 9, 16 -- Message-Id: {p06240800c55a4b6e6ca8-at-[76.234.167.108]} } } } 9, 16 -- In-Reply-To: } } } {200812020147.mB21lfgL007018-at-ns.microscopy.com} } } } 9, 16 -- References: {200812020147.mB21lfgL007018-at-ns.microscopy.com} } } } 9, 16 -- Date: Mon, 1 Dec 2008 21:25:47 -0500 } } } 9, 16 -- To: opmills-at-mtu.edu, Microscopy Listserver } } {microscopy-at-microscopy.com} } } } 9, 16 -- From: Wil Bigelow {bigelow-at-umich.edu} } } } 9, 16 -- Subject: Re: [Microscopy] viaWWW: eye glasses } } } 9, 16 -- Content-Type: text/plain; charset="us-ascii" ; } } } format="flowed" } } } ==============================End of - } } } Headers============================== } } } } } } } } } } } } } ==============================Original } } Headers============================== } } 12, 29 -- From beaurega-at-westol.com Tue Dec 2 10:10:24 2008 } } 12, 29 -- Received: from smtp-gateway-6.winbeam.com (smtp- } } gateway-6.winbeam.com [64.84.96.16]) } } 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id mB2GANbq009825 } } 12, 29 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 } } 10:10:24 -0600 } } 12, 29 -- Received: from mail.winbeam.com (mail.winbeam.com } } [64.84.96.10]) } } 12, 29 -- by smtp-gateway-6.winbeam.com (8.13.1/8.12.8) with SMTP } } id mB2GAHPE016696 } } 12, 29 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 } } 11:10:18 -0500 } } 12, 29 -- Received: (qmail 7256 invoked by uid 89); 2 Dec 2008 } } 16:10:00 -0000 } } 12, 29 -- Received: from pitts-69-72-13-133.dynamic- } } dialup.coretel.net (HELO running) (69.72.13.133) } } 12, 29 -- by mail.winbeam.com with SMTP; 2 Dec 2008 16:10:00 -0000 } } 12, 29 -- Message-Id: {3.0.6.32.20081202111025.0086aab0-at-pop3.norton.antivirus } } } } } 12, 29 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus } } 12, 29 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) } } 12, 29 -- Date: Tue, 02 Dec 2008 11:10:25 -0500 } } 12, 29 -- To: microscopy-at-microscopy.com } } 12, 29 -- From: Beaurega {beaurega-at-westol.com} } } 12, 29 -- Subject: Re: [Microscopy] Re: viaWWW: eye glasses } } 12, 29 -- In-Reply-To: } } {200812020226.mB22QIti012680-at-ns.microscopy.com} } } 12, 29 -- Mime-Version: 1.0 } } 12, 29 -- Content-Type: text/plain; charset="iso-8859-1" } } 12, 29 -- Content-Transfer-Encoding: 8bit } } 12, 29 -- X-Winbeam-MailScanner-Information: Winbeam - Please } } contact Technical Support for more information } } 12, 29 -- X-Winbeam-MailScanner-ID: mB2GAHPE016696 } } 12, 29 -- X-Winbeam-MailScanner: Found to be clean Winbeam } } (courtesy of MailScanner) } } 12, 29 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), } } 12, 29 -- SpamAssassin (not cached, score=-2, required 4, } } autolearn=not spam, } } 12, 29 -- BAYES_00 -2.00) } } 12, 29 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com } } 12, 29 -- X-Winbeam-MailScanner-Watermark: 1228839018.9997-at-wMITRQMua } } +Qyt7xu+nvSjA } } ==============================End of - } } Headers============================== } } { { { { { { { { {} } } } } } } } } } } } } } } Lou Ann Miller, Service Supervisor } Center for Microscopic Imaging } College of Veterinary Medicine } University of Illinois MC=002 } } Room 1204 VMBSBld } 2001 S Lincoln Ave } Urbana, IL 61821 } } 217-244-1567 } } } }
{ { { { { { { { {} } } } } } } } } } } } } } Lou Ann Miller, Service Supervisor Center for Microscopic Imaging College of Veterinary Medicine University of Illinois MC=002
Room 1204 VMBSBld 2001 S Lincoln Ave Urbana, IL 61821
217-244-1567
==============================Original Headers============================== 10, 18 -- From lamiller-at-illinois.edu Tue Dec 2 11:11:59 2008 10, 18 -- Received: from expredir5.cites.uiuc.edu (expredir5.cites.uiuc.edu [128.174.5.96]) 10, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2HBxgI017701 10, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 11:11:59 -0600 10, 18 -- Received: from beowulf.cvm.uiuc.edu (beowulf.cvm.uiuc.edu [130.126.16.163]) 10, 18 -- by expredir5.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id mB2HBwOg017073 10, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 11:11:58 -0600 (CST) 10, 18 -- Message-Id: {541FAD0E-BB26-411E-8AFA-618770DAF944-at-illinois.edu} 10, 18 -- From: Lou Ann Miller {lamiller-at-illinois.edu} 10, 18 -- To: Microscopy-at-microscopy.com 10, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 10, 18 -- Mime-Version: 1.0 (Apple Message framework v929.2) 10, 18 -- Subject: Fwd: [Microscopy] viaWWW: eye glasses 10, 18 -- Date: Tue, 2 Dec 2008 11:11:58 -0600 10, 18 -- References: {FF6A0A19-8F2D-4BFA-AB23-116757700149-at-illinois.edu} 10, 18 -- X-Mailer: Apple Mail (2.929.2) 10, 18 -- Content-Transfer-Encoding: 8bit 10, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2HBxgI017701 ==============================End of - Headers==============================
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Tuesday, December 02, 2008 9:13 AM To: dkloos-at-parallaxray.com
Hello,
We are currently renegotiating our service contract with the manufacturers of our SEMs. In order to ensure a fair appraisal of the proposal (we work for government), I need to find out if there are alternatives other than the original manufacturer.
Are you aware of any third party company specialized in SEM maintenance that would service two SEMs in Edmonton, Alberta, Canada?
Thank you for your help, Daniel
__________________________________________ Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC 11421 Saskatchewan Dr. Edmonton, AB. T6G 2M9
Phone: (780) 641-1663 Fax: (780) 641-1601
==============================Original Headers============================== 9, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Tue Dec 2 11:05:58 2008 9, 23 -- Received: from nrccenexf1.nrc.ca (nrccenexf1.nrc.ca [132.246.15.80]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2H5woi005502 9, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Dec 2008 11:05:58 -0600 9, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf1.nrc.ca with Microsoft SMTPSVC(6.0.3790.3959); 9, 23 -- Tue, 2 Dec 2008 12:05:57 -0500 9, 23 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: SEM third party maintenance 9, 23 -- Date: Tue, 2 Dec 2008 12:05:56 -0500 9, 23 -- Message-ID: {6AF37B759D23CD4FB862A21C1F06CF97613BD9-at-nrccenexb2.nrc.ca} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: SEM third party maintenance 9, 23 -- Thread-Index: AclUoD3jH6ZkONG7SlKC+l1nrFJ7PA== 9, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca} 9, 23 -- To: {Microscopy-at-Microscopy.Com} 9, 23 -- X-OriginalArrivalTime: 02 Dec 2008 17:05:57.0910 (UTC) FILETIME=[3EA26360:01C954A0] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2H5woi005502 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 28 -- From dkloos-at-parallaxray.com Tue Dec 2 11:43:37 2008 21, 28 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2Hhau2000747 21, 28 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 11:43:36 -0600 21, 28 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) 21, 28 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) 21, 28 -- (envelope-from {dkloos-at-parallaxray.com} ) 21, 28 -- id 1L7ZHf-000GAH-69; Tue, 02 Dec 2008 10:43:39 -0700 21, 28 -- From: "Don Kloos" {dkloos-at-parallaxray.com} 21, 28 -- To: {Daniel.Salamon-at-nrc-cnrc.gc.ca} 21, 28 -- Cc: {microscopy-at-microscopy.com} 21, 28 -- References: {200812021712.mB2HCmQT020163-at-ns.microscopy.com} 21, 28 -- Subject: RE: [Microscopy] SEM third party maintenance 21, 28 -- Date: Tue, 2 Dec 2008 09:43:32 -0800 21, 28 -- Message-ID: {F3B038BCFA4446249D69155E7BC83AFA-at-donl} 21, 28 -- MIME-Version: 1.0 21, 28 -- Content-Type: text/plain; 21, 28 -- charset="us-ascii" 21, 28 -- Content-Transfer-Encoding: 7bit 21, 28 -- X-Mailer: Microsoft Office Outlook 11 21, 28 -- Thread-Index: AclUoTVz0ZDWHTLeTnSS9XFyH/6GXwAA8XxQ 21, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 21, 28 -- In-Reply-To: {200812021712.mB2HCmQT020163-at-ns.microscopy.com} 21, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 21, 28 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com 21, 28 -- X-AntiAbuse: Original Domain - microscopy.com 21, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] 21, 28 -- X-AntiAbuse: Sender Address Domain - parallaxray.com ==============================End of - Headers==============================
Hi all, Has anyone used Luxel's film-coated grids? http://www.luxel.com/ The website says the film was developed with the help of NIH. Are they great? or not so great?
Thanks for your comments,
Beth
==============================Original Headers============================== 9, 19 -- From beth-at-plantbio.uga.edu Tue Dec 2 12:10:05 2008 9, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2IA5wj014652 9, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 12:10:05 -0600 9, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 9, 19 -- (authenticated user beth-at-plantbio.uga.edu) 9, 19 -- by dogwood.plantbio.uga.edu 9, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 9, 19 -- for microscopy-at-microscopy.com; 9, 19 -- Tue, 2 Dec 2008 13:10:00 -0500 9, 19 -- Message-Id: {7B44B7A2-A8D6-401E-AB3B-A04C2EE5C494-at-plantbio.uga.edu} 9, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 9, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 9, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit 9, 19 -- Mime-Version: 1.0 (Apple Message framework v929.2) 9, 19 -- Subject: Luxel film-coated grids 9, 19 -- Date: Tue, 2 Dec 2008 13:10:36 -0500 9, 19 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
They look cool on-line but they are $8.90 a piece at Ted Pella! Yikes.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Tuesday, December 02, 2008 12:11 PM To: Phillips, Thomas E.
Hi all, Has anyone used Luxel's film-coated grids? http://www.luxel.com/ The website says the film was developed with the help of NIH. Are they great? or not so great?
Thanks for your comments,
Beth
==============================Original Headers============================== 9, 19 -- From beth-at-plantbio.uga.edu Tue Dec 2 12:10:05 2008 9, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2IA5wj014652 9, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 12:10:05 -0600 9, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 9, 19 -- (authenticated user beth-at-plantbio.uga.edu) 9, 19 -- by dogwood.plantbio.uga.edu 9, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 9, 19 -- for microscopy-at-microscopy.com; 9, 19 -- Tue, 2 Dec 2008 13:10:00 -0500 9, 19 -- Message-Id: {7B44B7A2-A8D6-401E-AB3B-A04C2EE5C494-at-plantbio.uga.edu} 9, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 9, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 9, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit 9, 19 -- Mime-Version: 1.0 (Apple Message framework v929.2) 9, 19 -- Subject: Luxel film-coated grids 9, 19 -- Date: Tue, 2 Dec 2008 13:10:36 -0500 9, 19 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
==============================Original Headers============================== 19, 29 -- From PhillipsT-at-missouri.edu Tue Dec 2 12:14:36 2008 19, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 19, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2IEaSN024878 19, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 12:14:36 -0600 19, 29 -- X-IronPort-Anti-Spam-Filtered: true 19, 29 -- X-IronPort-Anti-Spam-Result: ApoEAPQJNUnRauUp/2dsb2JhbADGcQEJhnOFBQGBfoEA 19, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 19, 29 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 02 Dec 2008 12:14:35 -0600 19, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 19, 29 -- Tue, 2 Dec 2008 12:14:35 -0600 19, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5 19, 29 -- Content-class: urn:content-classes:message 19, 29 -- MIME-Version: 1.0 19, 29 -- Content-Type: text/plain; 19, 29 -- charset="us-ascii" 19, 29 -- Subject: RE: [Microscopy] Luxel film-coated grids 19, 29 -- Date: Tue, 2 Dec 2008 12:14:34 -0600 19, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD057A31EC-at-UM-XMAIL06.um.umsystem.edu} 19, 29 -- In-Reply-To: {200812021810.mB2IAZhH015760-at-ns.microscopy.com} 19, 29 -- X-MS-Has-Attach: 19, 29 -- X-MS-TNEF-Correlator: 19, 29 -- Thread-Topic: [Microscopy] Luxel film-coated grids 19, 29 -- Thread-Index: AclUqUaC9cMGmcxQStCsgObxbD+yzwAAGz1g 19, 29 -- References: {200812021810.mB2IAZhH015760-at-ns.microscopy.com} 19, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 19, 29 -- To: {Microscopy-at-microscopy.com} 19, 29 -- X-OriginalArrivalTime: 02 Dec 2008 18:14:35.0732 (UTC) FILETIME=[D50C2940:01C954A9] 19, 29 -- Content-Transfer-Encoding: 8bit 19, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2IEaSN024878 ==============================End of - Headers==============================
We were given some to try and they worked very well. Our users liked the increase in open viewing space. One thing we did find was that LR White sections did not adhere to them but epoxy ones were just fine.
Lesley
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Tuesday, December 02, 2008 1:16 PM To: Lesley Bechtold
Hi all, Has anyone used Luxel's film-coated grids? http://www.luxel.com/ The website says the film was developed with the help of NIH. Are they great? or not so great?
Thanks for your comments,
Beth
==============================Original Headers============================== 9, 19 -- From beth-at-plantbio.uga.edu Tue Dec 2 12:10:05 2008 9, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2IA5wj014652 9, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 12:10:05 -0600 9, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 9, 19 -- (authenticated user beth-at-plantbio.uga.edu) 9, 19 -- by dogwood.plantbio.uga.edu 9, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 9, 19 -- for microscopy-at-microscopy.com; 9, 19 -- Tue, 2 Dec 2008 13:10:00 -0500 9, 19 -- Message-Id: {7B44B7A2-A8D6-401E-AB3B-A04C2EE5C494-at-plantbio.uga.edu} 9, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 9, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 9, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit 9, 19 -- Mime-Version: 1.0 (Apple Message framework v929.2) 9, 19 -- Subject: Luxel film-coated grids 9, 19 -- Date: Tue, 2 Dec 2008 13:10:36 -0500 9, 19 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
==============================Original Headers============================== 17, 33 -- From Lesley.Bechtold-at-jax.org Tue Dec 2 12:18:40 2008 17, 33 -- Received: from ms01.jax.org (ms01.jax.org [209.222.206.170]) 17, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2IIedg003997 17, 33 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 12:18:40 -0600 17, 33 -- Received: from ms01.jax.org (localhost.localdomain [127.0.0.1]) 17, 33 -- by localhost (Email Security Appliance) with SMTP id 16F99135B091; 17, 33 -- Tue, 2 Dec 2008 18:18:40 +0000 (GMT) 17, 33 -- Received: from jaxbhex02.jax.org (unknown [10.43.200.45]) 17, 33 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) 17, 33 -- (No client certificate requested) 17, 33 -- by ms01.jax.org (Email Security Appliance) with ESMTP id 0613E135B078; 17, 33 -- Tue, 2 Dec 2008 18:18:38 +0000 (GMT) 17, 33 -- Received: from JAXBHMAIL01.jax.org ([10.43.200.39]) by jaxbhex02.jax.org 17, 33 -- ([10.43.200.45]) with mapi; Tue, 2 Dec 2008 13:18:36 -0500 17, 33 -- From: Lesley Bechtold {Lesley.Bechtold-at-jax.org} 17, 33 -- To: "beth-at-plantbio.uga.edu" {beth-at-plantbio.uga.edu} 17, 33 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 33 -- Date: Tue, 2 Dec 2008 13:18:35 -0500 17, 33 -- Subject: RE: [Microscopy] Luxel film-coated grids 17, 33 -- Thread-Topic: [Microscopy] Luxel film-coated grids 17, 33 -- Thread-Index: AclUqgeIGKM5FOHFTOmZqtWytrsQOwAACawg 17, 33 -- Message-ID: {3BDA51FFD1A83B4E90829F594A5C37172859BEC198-at-JAXBHMAIL01.jax.org} 17, 33 -- References: {200812021815.mB2IFwhV029813-at-ns.microscopy.com} 17, 33 -- In-Reply-To: {200812021815.mB2IFwhV029813-at-ns.microscopy.com} 17, 33 -- Accept-Language: en-US 17, 33 -- Content-Language: en-US 17, 33 -- X-MS-Has-Attach: 17, 33 -- X-MS-TNEF-Correlator: 17, 33 -- acceptlanguage: en-US 17, 33 -- Content-Type: text/plain; charset="us-ascii" 17, 33 -- MIME-Version: 1.0 17, 33 -- Content-Transfer-Encoding: 8bit 17, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2IIedg003997 ==============================End of - Headers==============================
I've worked with him off and on for years assisting with his ETEC Autoscans.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Tuesday, December 02, 2008 12:08 PM To: kenconverse-at-qualityimages.biz
Hello,
We are currently renegotiating our service contract with the manufacturers of our SEMs. In order to ensure a fair appraisal of the proposal (we work for government), I need to find out if there are alternatives other than the original manufacturer.
Are you aware of any third party company specialized in SEM maintenance that would service two SEMs in Edmonton, Alberta, Canada?
Thank you for your help, Daniel
__________________________________________ Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC 11421 Saskatchewan Dr. Edmonton, AB. T6G 2M9
Phone: (780) 641-1663 Fax: (780) 641-1601
==============================Original Headers============================== 9, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Tue Dec 2 11:05:58 2008 9, 23 -- Received: from nrccenexf1.nrc.ca (nrccenexf1.nrc.ca [132.246.15.80]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2H5woi005502 9, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Dec 2008 11:05:58 -0600 9, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf1.nrc.ca with Microsoft SMTPSVC(6.0.3790.3959); 9, 23 -- Tue, 2 Dec 2008 12:05:57 -0500 9, 23 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: SEM third party maintenance 9, 23 -- Date: Tue, 2 Dec 2008 12:05:56 -0500 9, 23 -- Message-ID: {6AF37B759D23CD4FB862A21C1F06CF97613BD9-at-nrccenexb2.nrc.ca} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: SEM third party maintenance 9, 23 -- Thread-Index: AclUoD3jH6ZkONG7SlKC+l1nrFJ7PA== 9, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca} 9, 23 -- To: {Microscopy-at-Microscopy.Com} 9, 23 -- X-OriginalArrivalTime: 02 Dec 2008 17:05:57.0910 (UTC) FILETIME=[3EA26360:01C954A0] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2H5woi005502 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 26 -- From kenconverse-at-qualityimages.biz Tue Dec 2 13:33:24 2008 23, 26 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 23, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2JXOeb024674 23, 26 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 13:33:24 -0600 23, 26 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta03.mail.rr.com 23, 26 -- with ESMTP 23, 26 -- id {20081202193323.HALB25405.cdptpa-omta03.mail.rr.com-at-Ken} ; 23, 26 -- Tue, 2 Dec 2008 19:33:23 +0000 23, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 23, 26 -- To: {Daniel.Salamon-at-nrc-cnrc.gc.ca} , 23, 26 -- "MSA Listserver" {microscopy-at-microscopy.com} 23, 26 -- Subject: RE: [Microscopy] SEM third party maintenance 23, 26 -- Date: Tue, 2 Dec 2008 14:33:17 -0500 23, 26 -- Message-ID: {834217D614C347BEB3C2A6EC74FD7835-at-Ken} 23, 26 -- MIME-Version: 1.0 23, 26 -- Content-Type: text/plain; 23, 26 -- charset="us-ascii" 23, 26 -- X-Priority: 3 (Normal) 23, 26 -- X-MSMail-Priority: Normal 23, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 23, 26 -- Importance: Normal 23, 26 -- Thread-Index: AclUoIbPDBVLp7T2QMSlJ/inHTYOGwAE7C0g 23, 26 -- In-Reply-To: {200812021708.mB2H806C009528-at-ns.microscopy.com} 23, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 23, 26 -- Content-Transfer-Encoding: 8bit 23, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB2JXOeb024674 ==============================End of - Headers==============================
Owen; Get a motorcycle to be cool. Don't let your wife design your glasses unless she is an optician. A lot depends on the nature of the correction you have. If you have severe presbyopia, you should get two prescriptions, one for close up and one for driving. When you try to accommodate too wide a range of focal lengths in progressives or bi/tri focals, the focal length gradients make looking through the lenses very difficult. Interestingly, every eye doctor I have ever been to did not know how to do this-I had to tell him what I wanted. If you have tiny lenses, the focal length gradient will be more severe no matter what. Besides, with the larger computer monitors common today, you will need a wider field of view. I also strongly recommend nitinol frames. Opticians call them titanium frames, but they are a TiNi alloy called nitinol, which is also well known as a shape memory alloy. Not only are these frames lighter and nearly indestructible, but they are extremely corrosion resistant, and are especially immune from salty perspiration running all over them. They do cost twice as much a regular frames, but the ones I am wearing right now are something like 15 years old, look new, and I have no plans on replacing them.
John Mardinly, Numonyx
-----Original Message----- X-from: opmills-at-mtu.edu [mailto:opmills-at-mtu.edu] Sent: Monday, December 01, 2008 5:49 PM To: MARDINLY, A
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both opmills-at-mtu.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] eye glasses
Question: All,
It's time to get new eyeglass frames and lens so I'm writing for some advice on the frame size. My wife insists that I need to incorporate the "cool factor" (my choice of words) in this purchase. The "cool" frames are vertically narrow (25 to 30 mm) and the optometrist says it is difficult to fit progressive lens in this dimension. Like many of you, I do a lot of close-up and computer work. I do not have trouble seeing distances over 6 feet. The doctor suggested it may be wise to have 2 sets of glasses, one for work and the other when I need to make a fashion statement. How have you all addresses this issue? Do the narrow lens work for microscopy? Do you have 2 pair? Other ideas or comments. Thanks in advance for your responses.
Dear List, Does anyone have--or know where to download--a program to calculate CTFs and envelope functions at various values of defocus into which I can input the appropriate instrument parameters? TIA for any help. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 2, 20 -- From tivol-at-caltech.edu Tue Dec 2 15:44:49 2008 2, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2LinwW022219 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 15:44:49 -0600 2, 20 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 2, 20 -- by fire-doxen-postvirus (Postfix) with ESMTP id EC049328A53 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 13:44:48 -0800 (PST) 2, 20 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 2, 20 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 2, 20 -- by fire-doxen-ssl (Postfix) with ESMTP id E8CD932899B 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 13:44:47 -0800 (PST) 2, 20 -- Message-Id: {94D9EE4C-E9D0-476F-8589-1E80FF5D1D93-at-caltech.edu} 2, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 20 -- Content-Transfer-Encoding: 7bit 2, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 2, 20 -- Subject: CTF and envelope functions 2, 20 -- Date: Tue, 2 Dec 2008 13:44:47 -0800 2, 20 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
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Hendrik O. Colijn colijn.1-at-osu.edu Campus Electron Optics Facility Ohio State University (614) 292-0674 http://www.ceof.ohio-state.edu Time is that quality of nature which keeps events from happening all at once. Lately it doesn't seem to be working.
==============================Original Headers============================== 11, 26 -- From colijn.1-at-osu.edu Tue Dec 2 16:25:00 2008 11, 26 -- Received: from er6s1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB2MP0H0003956 11, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 16:25:00 -0600 11, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 11, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x18 #31224) 11, 26 -- id {01N2MITVLPQO8X9VJM-at-ecr6.ohio-state.edu} for Microscopy-at-microscopy.com; 11, 26 -- Tue, 02 Dec 2008 17:24:59 -0500 (EST) 11, 26 -- Received: from HOC1.ecr6.ohio-state.edu 11, 26 -- (hoc1.ceof.ohio-state.edu [164.107.76.179]) by er6s1.ecr6.ohio-state.edu 11, 26 -- (PMDF V6.3-x18 #31224) 11, 26 -- with ESMTPA id {01N2MITVAJKS8WXTPE-at-ecr6.ohio-state.edu} ; Tue, 11, 26 -- 02 Dec 2008 17:24:58 -0500 (EST) 11, 26 -- Date: Tue, 02 Dec 2008 17:25:27 -0500 11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 26 -- Subject: Re: [Microscopy] CTF and envelope functions 11, 26 -- In-reply-to: {200812022146.mB2Lk08X024474-at-ns.microscopy.com} 11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 26 -- To: tivol-at-caltech.edu 11, 26 -- Cc: Microscopy-at-microscopy.com 11, 26 -- Message-id: {01N2MITVBL9A8WXTPE-at-ecr6.ohio-state.edu} 11, 26 -- MIME-version: 1.0 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 26 -- References: {200812022146.mB2Lk08X024474-at-ns.microscopy.com} ==============================End of - Headers==============================
I'm going to take this thread as an opportunity for a brief "public service" announcement.
If you are middle-aged and strongly nearsighted, do not neglect your eye exams! I've just been through surgery for a detached retina, and found out that severely myopic eyes are at greater risk with age. Learn from my mistake. I'm lucky that the repair appears to have been successful, but it ain't fun and full recovery will take a while. If caught early, a straightforward laser procedure with a one-day recovery often suffices.
Rick Mott, PulseTor LLC
==============================Original Headers============================== 9, 19 -- From rmott-at-pulsetor.com Tue Dec 2 16:39:42 2008 9, 19 -- Received: from smtpauth23.prod.mesa1.secureserver.net (smtpauth23.prod.mesa1.secureserver.net [64.202.165.47]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB2Mdf6A017696 9, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 16:39:41 -0600 9, 19 -- Received: (qmail 7547 invoked from network); 2 Dec 2008 22:39:41 -0000 9, 19 -- Received: from unknown (98.221.242.177) 9, 19 -- by smtpauth23.prod.mesa1.secureserver.net (64.202.165.47) with ESMTP; 02 Dec 2008 22:39:41 -0000 9, 19 -- Message-ID: {4935B92B.9020403-at-pulsetor.com} 9, 19 -- Date: Tue, 02 Dec 2008 17:39:39 -0500 9, 19 -- From: Rick Mott {rmott-at-pulsetor.com} 9, 19 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 9, 19 -- X-Accept-Language: en-us, en 9, 19 -- MIME-Version: 1.0 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- Subject: Re: [Microscopy] Re: viaWWW: eye glasses 9, 19 -- References: {200812021402.mB2E22IT009326-at-ns.microscopy.com} 9, 19 -- In-Reply-To: {200812021402.mB2E22IT009326-at-ns.microscopy.com} 9, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You don't say which maker and model SEMs you have. I would include that the contractor supply all parts and perform one or two PMs per year. If the SEMs are FE, then specify at least one tip change per year. Specifically identify consumables...apertures, etc. Make sure these are generally available.
Older SEMs are easier to fix as long as parts and docs are available. Specify response time from trouble call and then from response call to on-site.
Just a few things that come to mind.
gary g.
At 09:07 AM 12/2/2008, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Tue Dec 2 16:48:17 2008 10, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB2MmHKS031223 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 16:48:17 -0600 10, 20 -- Message-Id: {200812022248.mB2MmHKS031223-at-ns.microscopy.com} 10, 20 -- Received: (qmail 8498 invoked from network); 2 Dec 2008 14:47:05 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 8494, pid: 8495, t: 0.0737s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 20 -- by smtp2 with SMTP; 2 Dec 2008 14:47:05 -0800 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Tue, 02 Dec 2008 14:48:12 -0800 10, 20 -- To: Daniel.Salamon-at-nrc-cnrc.gc.ca 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] SEM third party maintenance 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200812021707.mB2H7H1R007959-at-ns.microscopy.com} 10, 20 -- References: {200812021707.mB2H7H1R007959-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Dear List, Thanks to all who replied with suggestions. Here is what I got:
} Try the attached Excel sheet. } } Henning Stahlberg,
I can send it, if Henning agrees, to those who might want it. }
} I know that CTFexplorer is useful: http://www.maxsidorov.com/ctfexplorer/ } } Andrea Nans
and Henk Colijn
} I am a Software Engineer/Researcher for a company called Media } Cybernetics, working on deconvolution software. } } Not sure if it is relevant but I have been researching transfer } functions of 3D aberration corrected STEM in order to deconvolve } images from this instrument. Our product has some routines for the } initial estimate of a CTF and then refines it as part of a blind } deconvolution process. Demo licenses are available. } } Brian Northan
} This one looks like it has all that you want but I've never tried it. } } http://ncmi.bcm.tmc.edu/homes/wen/ctf } } Craig.
from Craig Johnson and Angel Paredes
and, } I recommend Dr. Earl Kirkland's online java code. } } http://people.ccmr.cornell.edu/~kirkland/
from Huolin Xin. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 12, 20 -- From tivol-at-caltech.edu Tue Dec 2 20:04:35 2008 12, 20 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB324YrM018314 12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 20:04:34 -0600 12, 20 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 12, 20 -- by earth-doxen-postvirus (Postfix) with ESMTP id A3EF366E0A8C 12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 18:04:34 -0800 (PST) 12, 20 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 12, 20 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 12, 20 -- by earth-doxen-ssl (Postfix) with ESMTP id 7AD9566E065F 12, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 18:04:33 -0800 (PST) 12, 20 -- Message-Id: {EE8D5212-8894-40AB-844D-7C8C892804DE-at-caltech.edu} 12, 20 -- From: Bill Tivol {tivol-at-caltech.edu} 12, 20 -- To: microscopy-at-microscopy.com 12, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 12, 20 -- Content-Transfer-Encoding: 7bit 12, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 12, 20 -- Subject: Re: [Microscopy] CTF and envelope functions 12, 20 -- Date: Tue, 2 Dec 2008 18:04:33 -0800 12, 20 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Does anyone happen to know how to hook up the power from the transformer to a Hitachi S-510 SEM? We would like to try to turn on the SEM before we call for service of the SEM. We actually bought this unit from an auction and can't figure how to hook up the 3-wires to the unit. I realize it needs a 3.3kva power, which we have.
Thanks, Brian Chan MicroAssembly
==============================Original Headers============================== 3, 19 -- From bchan-at-microassembly.com Tue Dec 2 20:21:31 2008 3, 19 -- Received: from omr13.networksolutionsemail.com (omr13.networksolutionsemail.com [205.178.146.63]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB32LVrk031999 3, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Dec 2008 20:21:31 -0600 3, 19 -- Received: from mail.networksolutionsemail.com (ns-omr13.mgt.hosting.dc2.netsol.com [10.49.6.76]) 3, 19 -- by omr13.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id mB32LVv1016136 3, 19 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Dec 2008 21:21:31 -0500 3, 19 -- Received: (qmail 504 invoked by uid 78); 3 Dec 2008 02:21:31 -0000 3, 19 -- Received: from unknown (HELO ?192.168.1.114?) (bchan-at-microassembly.com-at-76.254.227.222) 3, 19 -- by ns-omr13.lb.hosting.dc2.netsol.com with SMTP; 3 Dec 2008 02:21:31 -0000 3, 19 -- Message-ID: {4935ED11.4010700-at-microassembly.com} 3, 19 -- Date: Tue, 02 Dec 2008 18:21:05 -0800 3, 19 -- From: Brian Chan {bchan-at-microassembly.com} 3, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 3, 19 -- MIME-Version: 1.0 3, 19 -- To: Microscopy-at-Microscopy.Com 3, 19 -- Subject: S-510 Power 3, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If it is set up for 240VAC, then you need one hot L1 and one hot L2 to go to the SEM. The (desirable) ground is the odd third (green) connection. But one hot L goes to one input pin and the other hot L goes to the other input pin.
Check all other appurtenances, EDS, WDS, etc. to make sure that they are being fed the correct voltage.
gary g.
At 06:22 PM 12/2/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Tue Dec 2 20:47:55 2008 11, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB32lsox013481 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 20:47:55 -0600 11, 20 -- Message-Id: {200812030247.mB32lsox013481-at-ns.microscopy.com} 11, 20 -- Received: (qmail 3095 invoked from network); 2 Dec 2008 18:46:43 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 3092, pid: 3093, t: 0.1042s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp2 with SMTP; 2 Dec 2008 18:46:43 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Tue, 02 Dec 2008 18:47:51 -0800 11, 20 -- To: bchan-at-microassembly.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] S-510 Power 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200812030222.mB32MZpP001975-at-ns.microscopy.com} 11, 20 -- References: {200812030222.mB32MZpP001975-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wjiang-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wjiang-at-gmail.com Name: Wen Jiang
Organization: Purdue University
Title-Subject: [Filtered] Electron Microscopist Position at Purdue
Question: An electron microscopist position is immediately available in the Jiang laboratory, Markey Center for Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. Purdue is well equipped with two FEI FEG cryo-microscopes (200kV and 300kV) and will install a new 300kV liquid He microscope in 2009. The applicant should have experience in high resolution TEM imaging. Experience in specimen preparation and low dose cryo-EM imaging of biological samples is preferred. The duties will be mainly on collecting cryo-EM images of viruses, large macromolecular complexes and nano-particles for postdoc and Ph.D. student researchers. The applicant should also be a team player that enjoys working in a collaborative environment. Please send CV, statement of cryo-EM experiences and interests, and references to Dr. Wen Jiang (jiang12-at-purdue.edu).
Wen Jiang, Ph.D. Assistant Professor Department of Biological Sciences Lilly Hall, B-402A Purdue University West Lafayette, IN 47907 Tel: 765-496-8436 (office) 765-496-9317 (lab) Fax: 765-496-1189 URL: http://jiang.bio.purdue.edu
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Email: mbisher-at-princeton.edu Name: Margaret Bisher
Organization: Princeton University
Title-Subject: [Filtered] Neurofilament Question
Question: I have a student who asked if I could post this question to the Histo-list.I told her how all of you seem to be very helpful and perhaps some of you know the answer to her question:
Dear all,
I am looking for a good "catch-all" antibody for neuronal intermediate filaments (NF-L, NF-M and NF-H). Since they're generally expressed together in each neurofilament I'm not interested in any one of them specifically, but want to avoid using a cocktail of antibodies, as this could get expensive. Basically, I just want to show that a subset of axons contains neurofilaments with a reliable antibody, but the particular protein or phosphorylation state doesn't matter. Any suggestions you could offer would be incredibly helpful!
Sincerely, Eve Schneider
Thanks,
Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher-at-princeton.edu
I labeled a substance with an Alexa488 dye. Now I would like to see its internalization in cells in culture. My main issue is that I have no confocal microscope, so I have to find a way to eliminate the extracellular fluorescence and keep only the fluorescence inside the cells. I know that it is possible to quench the fluorescence with Trypan blue, but I don't understand exactly how it works. Does trypan blue stick to the dye and quench it, so that even after washing the fluorescence is still quenched? Or do you have to leave the trypan blue is solution (I wonder how it can influence imaging)? Does someone have instructions to do that? How long, how diluted? Is the pH important/critical? How long is the quenching stable?
Best regards,
Stephane
==============================Original Headers============================== 7, 21 -- From nizets2-at-yahoo.com Wed Dec 3 02:46:47 2008 7, 21 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB38klZg019479 7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 02:46:47 -0600 7, 21 -- Received: (qmail 15706 invoked by uid 60001); 3 Dec 2008 08:46:47 -0000 7, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 21 -- s=s1024; d=yahoo.com; 7, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 21 -- b=dtPanKLeP4IdvCMc+T/hq6WNkwPVlWvIPf+7lyISKtl9N9dcBzJstZBZBeJWzYbQAs5rxTFiF6Ywe1yDn//1iJl/qn3BOEikuhAVONHABGtu0FW7XQwsnKrTO/8BG6S67+v6CCjHXw33Yg5KFWxo8X/koL2+O808LWCW0//Voc4=; 7, 21 -- X-YMail-OSG: 7eXprgMVM1mN1TUJnDpgZwgoA.w0DfERob.LQymXCnQIgnv7v23PxWxIaZXyWvumWNpD2ZwG3qwoAqzGqGQ4BeRPy9m6MWgG0WtkW2C_qkX04tn.dl3D2kO.GUIkmFWlhdl7jlQTPJTIHa5CZfZ4te7yPzIBcR8m8mHdxAgR 7, 21 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Wed, 03 Dec 2008 00:46:46 PST 7, 21 -- X-Mailer: YahooMailRC/1155.32 YahooMailWebService/0.7.260.1 7, 21 -- Date: Wed, 3 Dec 2008 00:46:46 -0800 (PST) 7, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 21 -- Subject: fluorescence quenching 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=iso-8859-1 7, 21 -- Message-ID: {32193.15254.qm-at-web37406.mail.mud.yahoo.com} 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB38klZg019479 ==============================End of - Headers==============================
Haven't seen a reply to your question, so I've quickly modified a few of my replies to similar questions :
For GFP fluorescence microscopy, one would normally choose a Peltier cooled black and white B&W CCD camera, as this are very sensitive and very low noise - the latter also being very important with the relatively low light coming from GFP [epi-fluorescence imaged] samples. Peltier cooling allows for very low noise amplification [image intensifying] of dim light levels. Although say the DAPI, FITC/GFP and TRITC images are captured in greyscale with these sensitive cameras, you can apply the blue, green, red [RGB] emission colour afterwards via 'look-up tables' [LUTs]. Generally you get some software as well to acquire images, apply the LUTs, and possibly do some image analysis [although freebie ImageJ can do this].
These fluorescence cameras are very expensive though, costing many thousands of pounds [not such a big deal with a £30,000+ multi-user Core microscope, but it depends on what type of setup you are thinking off - fluorescence stereo dissection microscopes can be bought, but again they aren't cheap and aren't as commonplace as compound fluorescence microscope versions used for flat specimens]. CMOS sensors are creeping into photomicroscopy, but image noise is still a real problem at these low light levels [but they are cheaper to manufacture and so buy, and are OK with bright-field, e.g. stained sections, when you can just turn up the transmission lamp brightness]. See:
http://www.dalsa.com/markets/ccd_vs_cmos.asp http://www.imaging-resource.com/PRODS/D30/D30A4.HTM The above ignores noise, which fluorescence microscopists cannot do
The uniformity of CCD chips in the camera is important for microscopy imaging, as is the use of plan fluorescence objectives optimised for use with low light fluorescence [Fluar], uV & far red, and these must be uniformly in-focus across the field of view [i.e. have plan optics].
A hi-sensitivity Peltier cooled fluorescence camera [e.g. Hamamatsu Orca ER] is only a megapixel or so, but it scores by being very low noise and very sensitive - hence people paid it's £10k price tag. It can also do things like binning 4 pixels 'into' 1 for 4x higher sensitivity with low noise. Plus it's only B&W so no red/green/ blue pixels and Bayer mosaic processing to worry about. My Olympus SLR might have a massive 10 mega-pixel resolution but that's not what required for rapid low noise picture capture of fluorescence specimens [my SLR's image intensified noise on the photo is a disaster for detail, even when used in a well lit room]. The 1 megapixel is also OK as with microscopes as you simply increase resolution by changing objective and perhaps using an optical zoom as well.
Similarly for colour capture you need a decent microscope colour camera rather than a video camera, and then you have to get into VDU colour correction, transmission lamp bulb spectral response, white balance correction, etc... - all a bit too much to go into on the list-server. These are a lot cheaper than B&W low-noise fluorescence cameras, and have many more pixels (although they are spilt three ways colour wise).
Generally I just call up our local microscope rep for that system, and select one of their recommended cameras [which may not be their own brand], as they are pretty clued up on this area and if you run into problems, one manufacturer can't blame the other for any resulting problems (which can run on for years). If they don't know what works best with their microscopes they would be out of business these days.
If you aren't that bothered about absolute image quality, or quantifying image intensities and areas, then a standard colour camera attached to a microscope can be a cheap alternative for pretty pictures of bright-field samples - again manufacturers have recommended digital cameras and port adapters [Leica and Zeiss even make 'cheap' USB colour camera models]. But this often simply isn't good enough for decent low-light fluorescence image acquisition.
There are many second party microscope camera suppliers that may undercut microscope manufacturers prices, and these can supply decent cameras [although broadly speaking you get what you pay for, although some cameras may offer better value and/or specific features/software you desire]. But given the high cost of microscope imaging and sample preparation, I would make sure that I don't fall at the last hurdle.
Most B&W fluorescence & colour cameras are PC friendly USB2/FireWire these days, even if they have a separate camera power supply. If you haven't got more than £2,000 for a Peltier cooled camera you will have to compromise on image quality, and you should still get a pretty decent image with a very bright DAPI and GFP specimen, provided you don't look too closely at the detail [although that depends on objective type and it's mag as well]. You would look for word like 'low noise' and 'for fluorescence'. I haven't any experience of these cheaper camera models [other list-servers will no doubt be able to offer help, if you are specific to cost, microscope and imaging requirements]
Things are always changing though in the digital camera and microscopy world, and it's been a couple of years since I have actually bought a microscope imaging camera.
Keith
-------------------------------------------------------------------------- Dr Keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: wallacep-at-science.oregonstate.edu [mailto:wallacep-at-science.oregonstate.edu] Sent: 30 November 2008 06:13 To: kjmorris-at-well.ox.ac.uk
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Sorry to bother everyone but it seemed the best way to reach those who might need to be reached.
A phone outage occurred in Vermont last night and this morning. If anyone has been unable to contact Ladd Research via phone, fax, e-mail or our website in the past 12 hours or so please be advised that you can try again now as everything is back up and running
On Dec 3, 2008, at 12:46 AM, nizets2-at-yahoo.com wrote:
} I know that it is possible to quench the fluorescence with Trypan } blue, but I don't understand exactly how it works. } Does trypan blue stick to the dye and quench it, so that even after } washing the fluorescence is still quenched? } Or do you have to leave the trypan blue is solution (I wonder how it } can influence imaging)?
Dear Stephane, There are several possible ways that fluorescence can be quenched, and I do not know how trypan blue works. Some compounds provide a non- radiative pathway for decay of the fluorophor from the excited state to the ground state. This can be either by binding or by being near enough to have a high probability of energy transfer--think FRET without the shifted photon. Other compounds change the environment, especially hydrophobicity or hydrophyllicity, which only work when fluorophors require a specific environment to fluoresce. Some fluorophors, for example, emit only when intercalated into membranes or DNA, etc. The details of how to continue to have Alexa488 continue to fluoresce within the cells, but not have other intracellular fluorescence and how Alexa488 interacts with trypan blue, I'll leave to the experts. Washing out Alexa488 that is not within your cells should be pretty straightforward and will eliminate extracellular fluorescence, but, again, take an expert's opinion over mine. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Dec 3 14:04:54 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3K4s0x015486 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 14:04:54 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 58420328A7D 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 12:04:54 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 3588B328A05 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 12:04:53 -0800 (PST) 6, 22 -- Message-Id: {7E229924-C9B0-466C-852A-4DBFBF13EDD4-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200812030846.mB38kvmC019607-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] fluorescence quenching 6, 22 -- Date: Wed, 3 Dec 2008 12:04:53 -0800 6, 22 -- References: {200812030846.mB38kvmC019607-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
Hi. Here at Merritt College we just started a formal training program in microscopy!!! We were inspired by the excellent program at SJDC, but we focus on optical microscopy (no EM yet). As to microscopy training, I think there's only 3 actual full scale programs in the country/world: SJDC, the similar program in Michigan (started by a grad of SJDC, I think) and us! Lehigh U. has some great courses, mostly short-term, UC Berkeley has a few excellent courses for undergrads, and there's the Pawley 2 week course every June in Canada, plus two Woods Hole summer intensives (excellent training). Barbara Foster does training and wrote a great book and the scope co's have lots of in-house training.
Our program is just one year old. Our first cohort of students is getting ready to graduate in March and they're available for hire! They're amazing people, really smart, motivated, hard working and well trained!
Check out our website: www.merritt.edu/microscopy. It's a little out of date in some of the details, but the jist of it is correct. We have a slew of great microscopes (including 4 motorized widefield systems and 2 confocals) and train our students both in the theory and the practice of transmitted light and fluorescence techniques, along with general lab and business skills. Our students come from a wide variety of backgrounds, but most have had other careers already (including in photography, computers, etc.). We're training folks for careers in imaging cores, research labs, biotech, sales, and so forth.
I'd love to get feedback, ideas, comments, etc. from this listserve, so please email me if you have any ideas, or would like to connect with the program in any way. We have an advisory board that guides us, and I'm always interested in new members and in input from the community, not to mention job leads, supplies, collaborations, curriculum resources, etc.
In fact, I have some openings for part-time instructors this spring (and beyond) for the intro, very basic level course on optical microscopy and a short-term (sundays in feb) course in good lab practices. Please contact me if you're interested!
Also, I have three top students who did international internships, but are looking for local internships right now, if any of you needs an extra pair of hands!
Save the date: we're having an open house on Friday, Jan. 23rd, from 4-7:30. There will be tours, demos, student presentations. All ages and interest levels are welcome! Feel free to bring your own specimen to image.
regards, Dr. Gisele Giorgi Director, MMP ggiorgi-at-peralta.edu tiger3g3-at-yahoo.com www.merritt.edu/microscopy
==============================Original Headers============================== 9, 25 -- From tiger3g3-at-yahoo.com Wed Dec 3 14:11:16 2008 9, 25 -- Received: from n24.bullet.mail.mud.yahoo.com (n24.bullet.mail.mud.yahoo.com [68.142.206.163]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB3KBGoS022560 9, 25 -- for {microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 14:11:16 -0600 9, 25 -- Received: from [209.191.108.96] by n24.bullet.mail.mud.yahoo.com with NNFMP; 03 Dec 2008 20:11:16 -0000 9, 25 -- Received: from [68.142.201.247] by t3.bullet.mud.yahoo.com with NNFMP; 03 Dec 2008 20:11:16 -0000 9, 25 -- Received: from [127.0.0.1] by omp408.mail.mud.yahoo.com with NNFMP; 03 Dec 2008 20:11:16 -0000 9, 25 -- X-Yahoo-Newman-Id: 431750.58195.bm-at-omp408.mail.mud.yahoo.com 9, 25 -- Received: (qmail 44815 invoked from network); 3 Dec 2008 20:11:16 -0000 9, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 25 -- s=s1024; d=yahoo.com; 9, 25 -- h=Received:X-YMail-OSG:X-Yahoo-Newman-Property:Mime-Version:X-Sender:Message-Id:Date:To:From:Subject:Content-Type; 9, 25 -- b=anjzFOvvxlbrywNzvaJZoD7w03yl76rZGqkJayWqAByHJbuVsOjYkoS3j1ssNUFCY/yum5vZS/B1woxhYG2dQpuaOd1IEk6jaW1RMwfpEGlzOgM30ZibQKRNHxn7eLoutgJM4D426FJ6issAE7J/h6OnhjOuRozxSXGlmpnZqrA= ; 9, 25 -- Received: from unknown (HELO ?98.207.93.115?) (tiger3g3-at-98.207.93.115 with plain) 9, 25 -- by smtp108.plus.mail.sp1.yahoo.com with SMTP; 3 Dec 2008 20:11:15 -0000 9, 25 -- X-YMail-OSG: ElaZlwAVM1mYNoQ1i5d4yhiOHQWEDTQavMCKBpjqhLH_3ZdaAcAOxw2pZQDhCwGWPz5ENEzxT8tkY2xZ13APBfFzQhMrUJIcyaDdAsxkaWN.Q1ru2jMEfu2D4rQmEqqB2loq5FL9Ok6h0h432c.rNYDXP_Njj9hGzF3Ly9Zzb8_DAG6EUMdNByDESw-- 9, 25 -- X-Yahoo-Newman-Property: ymail-3 9, 25 -- Mime-Version: 1.0 9, 25 -- X-Sender: tiger3g3-at-pop.mail.yahoo.com 9, 25 -- Message-Id: {a05111b28c55c93532ce9-at-[98.207.93.115]} 9, 25 -- Date: Wed, 3 Dec 2008 12:11:14 -0800 9, 25 -- To: microscopy-at-microscopy.com 9, 25 -- From: Gisele Eliane Giorgi {tiger3g3-at-yahoo.com} 9, 25 -- Subject: Merritt Microscopy Program 9, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
An old friend recently sent me the following question:
"Would three different SEMs, with the very same leak size in the transition range, and same chamber volumes, but each with different TMP pumping speeds, say 100, 300 & 500 l/s, respectively, have the same ultimate pressure?"
And since it is a matter of rather wide interest, I thought you-all might be interested in the answer, which is as follows:
The simple answer to your question is NO! The 500 l/s pump would definitely achieve a lower ultimate pressure.
Justification of this answer is a bit more complicated. If you will refer to my book on Vacuum Methods in Electron Microscopy, there are two equations that are particularly relevant to this question. The first is equation 2.16 on page 48, which shows that the ultimate pressure Pu is given by the ratio of the rate of gas influx q to the speed of evacuation Se (i.e. Pu = q/Se). The second is equation 2.12 on page 40, and the graph on page 41, that show that the speed of evacuation Se is strongly dependent on both the speed of the pump Sp and the conductance C of the line connecting the pump to the chamber; that is: [Se = (Sp x C)/(Sp + C)].
Thus, since speed of evacuation is usually strongly dependent on the speed of the pump, equation 2.16 suggests that, for a fixed leak rate q, and everything else being equal, the ultimate pressure would be lower for the system with the pump having the highest pumping speed Sp; namely, the. 500 l/s pump.
However, it is unlikely that all other things would be equal, because the smaller pumps have smaller diameters, and therefore are usually fitted with tubing leading to the chamber that is smaller in diameter than the larger pumps. This usual practice of using smaller diameter tubes with the smaller pumps would lead to a decrease in conductance C for those systems, and combined with the lower pumping speeds Sp of their pumps would normally cause the systems involving the smaller pumps to have very significantly lower speeds of evacuation Se than the larger ones, further increasing the difference in ultimate pressure attainable.
From an operational point of view it is also interesting to note that most SEMs can be brought into operation at some operating pressure Po that is considerably higher than the ultimate pressure attainable in their vacuum systems. Equation 2.19 indicates that this pumpdown time t is inversely proportional to the speed of evacuation. Therefore, if all other things are equal, this pumpdown time would also be much shorter for the larger pumps. This is a matter of considerable importance to operators of the instruments, because it determines how long they need to wait before turning on the high voltage after specimen exchange, etc.
HAPPY HOLIDAYS, EVERYONE
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 11, 14 -- From bigelow-at-umich.edu Wed Dec 3 14:13:12 2008 11, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 11, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3KD6XQ028025 11, 14 -- for {microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 14:13:07 -0600 11, 14 -- Received: FROM [141.212.131.153] (bigelow-imac-g5.engin.umich.edu [141.212.131.153]) 11, 14 -- BY hellskitchen.mr.itd.umich.edu ID 4936E851.5BAB3.11417 ; 11, 14 -- 3 Dec 2008 15:13:05 -0500 11, 14 -- Mime-Version: 1.0 11, 14 -- Message-Id: {p06240802c55c8ed6452b-at-[141.212.131.153]} 11, 14 -- Date: Wed, 3 Dec 2008 15:13:04 -0500 11, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 11, 14 -- Subject: [Microscopy]Pump speed vs Ult. press 11, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I have been reading the thread on fluorescence quenching with interest, and wonder if anyone can give a protocol for using trypan blue (all the books say it is minimally soluble in water....)
Thanks in advance
shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1760 Facsimile/Télécopieur: 613-759-1701 Rm 2068 K.W. Neatby Bldg 960 Carling Ave. Central Experimental Farm Ottawa, ON K1A 0C6 millers-at-agr.gc.ca
==============================Original Headers============================== 10, 21 -- From MILLERS-at-AGR.GC.CA Wed Dec 3 14:54:12 2008 10, 21 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3KsB0f024327 10, 21 -- for {Microscopy-at-Microscopy.Com} ; Wed, 3 Dec 2008 14:54:11 -0600 10, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 21 -- Content-class: urn:content-classes:message 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: text/plain; 10, 21 -- charset="iso-8859-1" 10, 21 -- Subject: fluorescence quenching 10, 21 -- Date: Wed, 3 Dec 2008 15:54:11 -0500 10, 21 -- Message-ID: {FD493497DF264A4E8DC71F1FFE49129DD57744-at-ONOTTAXMS2.AGR.GC.CA} 10, 21 -- X-MS-Has-Attach: 10, 21 -- X-MS-TNEF-Correlator: 10, 21 -- Thread-Topic: fluorescence quenching 10, 21 -- Thread-Index: AclViUrkG3JRZihUSDmfu5qf8f2PpA== 10, 21 -- From: "Miller, Shea" {MILLERS-at-AGR.GC.CA} 10, 21 -- To: {Microscopy-at-Microscopy.Com} 10, 21 -- X-OriginalArrivalTime: 03 Dec 2008 20:54:11.0570 (UTC) FILETIME=[4B1AD520:01C95589] 10, 21 -- Content-Transfer-Encoding: 8bit 10, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB3KsB0f024327 ==============================End of - Headers==============================
Image Core Facility, at Skirball Institute of New York University School of Medicine is seeking a full-time staff member. The Image core facility provides state-of-the-art imaging and visualization resources for the School of Medicine and local scientific community.
We seek a dynamic, motivated individual with expertise in current techniques for fluorescence imaging of cells and tissues. Candidates must have demonstrated expertise in confocal microscopy in fixed and live cells (tissues). Experience with software for image analysis and data presentation is essential. Minimal requirements include a bachelor degree in scientific field, and 2-5years experience in advanced fluorescence microscopy imaging techniques. Masters degree in biology is preferred, and specific experience in electron microscopy is highly desirable. The successful candidate will coordinate the operation of the light microscopy resources of the image core facility, train users in microscope operation and advanced imaging techniques, and assist sample preparation of electron microscopy.
To apply, a curriculum vita, a cover letter including statement of interests, salary requirements and contact information for three references should be e-mailed to: Fengxia.Liang-at-med.nyu.edu.
Alice
-- Alice F. Liang, Ph.D. Director, Image Core Facility Skirball Institute of Biomolecular Medicine Skirball 2nd floor, EM Suite New York University School of Medicine New York, NY 10016 Tel: 212-263-7644 (o); 212-263-7099 (Lab) Fax: 212-263-7643 E-mail: Fengxia.Liang-at-med.nyu.edu http://saturn.med.nyu.edu/facilities/imagecore/
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==============================Original Headers============================== 7, 24 -- From Fengxia.Liang-at-med.nyu.edu Wed Dec 3 15:24:01 2008 7, 24 -- Received: from mail3.nyumc.org (mail3.nyumc.org [216.165.126.230]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3LO0Y0006046 7, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 15:24:00 -0600 7, 24 -- X-IronPort-AV: E=Sophos;i="4.33,710,1220241600"; 7, 24 -- d="scan'208";a="46234877" 7, 24 -- Received: from msgwsdcpbh61.nyumc.org ([10.147.36.107]) 7, 24 -- by mail3.nyumc.org with ESMTP; 03 Dec 2008 16:24:00 -0500 7, 24 -- Received: from MSGWSDCPMB02.nyumc.org ([10.147.37.225]) by msgwsdcpbh61.nyumc.org with Microsoft SMTPSVC(6.0.3790.3959); 7, 24 -- Wed, 3 Dec 2008 16:23:59 -0500 7, 24 -- Received: from 128.122.135.9 ([128.122.135.9]) by MSGWSDCPMB02.nyumc.org ([10.147.37.24]) via Exchange Front-End Server mail.nyumc.org ([10.134.254.78]) with Microsoft Exchange Server HTTP-DAV ; 7, 24 -- Wed, 3 Dec 2008 21:24:00 +0000 7, 24 -- User-Agent: Microsoft-Entourage/12.11.0.080522 7, 24 -- Date: Wed, 03 Dec 2008 16:22:14 -0500 7, 24 -- Subject: Position opening 7, 24 -- From: Alice Liang {Fengxia.Liang-at-med.nyu.edu} 7, 24 -- To: {Microscopy-at-microscopy.com} 7, 24 -- Message-ID: {C55C62B6.2EE3A%Fengxia.Liang-at-med.nyu.edu} 7, 24 -- Thread-Topic: Position opening 7, 24 -- Thread-Index: AclVjTXpZqOTvJkxT6Soi0jp+AyRKQ== 7, 24 -- Mime-version: 1.0 7, 24 -- X-OriginalArrivalTime: 03 Dec 2008 21:23:59.0530 (UTC) FILETIME=[74CFE0A0:01C9558D] 7, 24 -- Content-Transfer-Encoding: 7bit 7, 24 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Your experience seems to be different from mine. All too often, when a manufacturer decides that a higher pumping speed is needed, they just add a bigger pump. But they do not redesign the pumping line to match. For example, they are unlikely to make a bigger pumping port into the sample chamber. The pumping speed (where you need it) and the ultimate pressure are then controlled by the conductance. The result of this - as your equation would show - is that putting on the bigger pump hardly affects the pumping speed.
Alwyn Eades
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 5, 21 -- From jae5-at-lehigh.edu Wed Dec 3 15:41:12 2008 5, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3LfAoD019746 5, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 15:41:12 -0600 5, 21 -- Received: from [127.0.0.1] (Dyn055140.mat.Lehigh.EDU [128.180.55.140]) 5, 21 -- (authenticated bits=0) 5, 21 -- by rain.cc.lehigh.edu (8.14.3/8.14.3) with ESMTP id mB3Lf8kR001379 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Dec 2008 16:41:08 -0500 5, 21 -- Message-ID: {4936FCF8.4020308-at-lehigh.edu} 5, 21 -- Date: Wed, 03 Dec 2008 16:41:12 -0500 5, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 5, 21 -- Organization: Lehigh University 5, 21 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 5, 21 -- MIME-Version: 1.0 5, 21 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 5, 21 -- Subject: Pump speed vs Ult. press 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Virus-Scanned: ClamAV 0.94.2/8718/Wed Dec 3 08:29:03 2008 on rain.cc.lehigh.edu 5, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Rick; Sorry to hear about your detached retina. There is another occurrence to boomers with over 4 diopters of myopia, which is a PVD (posterior vitreous detachment), which I had last year. This is a detachment of the vitreous humor from the retina which results in the Mother of all floaters. Risk factors, as were related to me by the doctor, were age, myopia and use of steroidal inhalers (like Advair) for asthma.
John Mardinly, Numonyx
-----Original Message----- X-from: rmott-at-pulsetor.com [mailto:rmott-at-pulsetor.com] Sent: Tuesday, December 02, 2008 2:45 PM To: MARDINLY, A
I'm going to take this thread as an opportunity for a brief "public service" announcement.
If you are middle-aged and strongly nearsighted, do not neglect your eye exams! I've just been through surgery for a detached retina, and found out that severely myopic eyes are at greater risk with age. Learn from my mistake. I'm lucky that the repair appears to have been successful, but it ain't fun and full recovery will take a while. If caught early, a straightforward laser procedure with a one-day recovery often suffices.
Rick Mott, PulseTor LLC
==============================Original Headers============================== 9, 19 -- From rmott-at-pulsetor.com Tue Dec 2 16:39:42 2008 9, 19 -- Received: from smtpauth23.prod.mesa1.secureserver.net (smtpauth23.prod.mesa1.secureserver.net [64.202.165.47]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB2Mdf6A017696 9, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Dec 2008 16:39:41 -0600 9, 19 -- Received: (qmail 7547 invoked from network); 2 Dec 2008 22:39:41 -0000 9, 19 -- Received: from unknown (98.221.242.177) 9, 19 -- by smtpauth23.prod.mesa1.secureserver.net (64.202.165.47) with ESMTP; 02 Dec 2008 22:39:41 -0000 9, 19 -- Message-ID: {4935B92B.9020403-at-pulsetor.com} 9, 19 -- Date: Tue, 02 Dec 2008 17:39:39 -0500 9, 19 -- From: Rick Mott {rmott-at-pulsetor.com} 9, 19 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 9, 19 -- X-Accept-Language: en-us, en 9, 19 -- MIME-Version: 1.0 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- Subject: Re: [Microscopy] Re: viaWWW: eye glasses 9, 19 -- References: {200812021402.mB2E22IT009326-at-ns.microscopy.com} 9, 19 -- In-Reply-To: {200812021402.mB2E22IT009326-at-ns.microscopy.com} 9, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 17, 29 -- From A.MARDINLY-at-numonyx.com Wed Dec 3 16:07:44 2008 17, 29 -- Received: from smtp2.whdoakpoyel002.gmessaging.net (mail2.numonyx.com [57.77.12.38]) 17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3M7hEk001225 17, 29 -- for {Microscopy-at-Microscopy.com} ; Wed, 3 Dec 2008 16:07:44 -0600 17, 29 -- Received: from exdresfenmx01.numonyx.local (unknown [10.96.252.22]) 17, 29 -- by smtp2.whdoakpoyel002.gmessaging.net (Postfix) with ESMTP id 662CF24807F; 17, 29 -- Wed, 3 Dec 2008 16:44:35 -0500 (EST) 17, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx01.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 17, 29 -- Wed, 3 Dec 2008 17:07:42 -0500 17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 29 -- Content-class: urn:content-classes:message 17, 29 -- MIME-Version: 1.0 17, 29 -- Content-Type: text/plain; 17, 29 -- charset="us-ascii" 17, 29 -- Subject: RE: [Microscopy] viaWWW: Near Sightedness and Retina Problems 17, 29 -- Date: Wed, 3 Dec 2008 17:07:18 -0500 17, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9BAB8CD-at-EXDRESBENMX012.numonyx.local} 17, 29 -- In-Reply-To: {200812022244.mB2MicgV029412-at-ns.microscopy.com} 17, 29 -- X-MS-Has-Attach: 17, 29 -- X-MS-TNEF-Correlator: 17, 29 -- Thread-Topic: [Microscopy] viaWWW: Near Sightedness and Retina Problems 17, 29 -- Thread-Index: AclUz6QprMjUwu/xQUaiN1RlEfx4rgAwiLsQ 17, 29 -- References: {200812022244.mB2MicgV029412-at-ns.microscopy.com} 17, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 17, 29 -- To: {rmott-at-pulsetor.com} 17, 29 -- Cc: {Microscopy-at-Microscopy.com} 17, 29 -- X-OriginalArrivalTime: 03 Dec 2008 22:07:42.0780 (UTC) FILETIME=[90640FC0:01C95593] 17, 29 -- Content-Transfer-Encoding: 8bit 17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB3M7hEk001225 ==============================End of - Headers==============================
Generally it is auto-fluorescence in the cells [or tissue] that you wish to quench - you normally don't have loads of fluorochrome fluorescing in the culture/mounting media as such [you replace the media prior to imaging, remove it during washes, and say use a clear CO2 independent culture media for short time-lapse of live cells rather than use the standard red tinted culture media stuff that happily autofluoresces a bit].
We used microscope flow cells and micro-injectors back at UCL, and I don't remember getting serious background fluorescence problems [shame I'm not there now as I'm sure my old Cell Biology colleagues could offer loads of advice, but my job was just to make sure the microscope/flow cell/Eppendorf injectors worked perfectly].
I have used the excellent Wright Cell Imaging Facility's article on auto fluorescence many times and it's linked here [along with some other useful auto fluorescence related stuff]. It discusses your problem.
Except the main link doesn't work, so get it direct from: http://www.uhnresearch.ca/facilities/wcif/PDF/Autofluorescence.pdf
And just search 'auto fluorescence', 'autofluorescence', 'auto-fluorescence', and 'reducing', 'quenching', 'eliminating' and so on......
Although it's not 'autofluorescence' you are trying to remove, the info will still be relevant] - some of it might be aimed at quenching autofluorecence inside the cell though.
Regards
Keith
Note: Amongst other things, Trypan blue is used to quench auto-fluorescence - never actually tried it as generally, say with eye sections/cells, we use the autofluorecence to our advantage and actually don't want it to fade.
With a confocal you can just about image anything that is or has lived using autofluorescence, it's just that generally the autofluorescence brightness is well below the fluorochrome you have added, so you never see it. You simply image unstained cells/tissue as well to check whether autofluorecence was a problem at the imaging gain/laser power or camera exposure you use with the fluorochrome sample].
Some cells are easy to identify owing to their bright autofluorescence, e.g. Autofluorescence in our eye's rods and cones can be used to detect the early onset of eye disease etc..
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: MILLERS-at-AGR.GC.CA [mailto:MILLERS-at-AGR.GC.CA] Sent: 03 December 2008 20:59 To: kjmorris-at-well.ox.ac.uk
Hi;
I have been reading the thread on fluorescence quenching with interest, and wonder if anyone can give a protocol for using trypan blue (all the books say it is minimally soluble in water....)
Thanks in advance
shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1760 Facsimile/Télécopieur: 613-759-1701 Rm 2068 K.W. Neatby Bldg 960 Carling Ave. Central Experimental Farm Ottawa, ON K1A 0C6 millers-at-agr.gc.ca
==============================Original Headers============================== 10, 21 -- From MILLERS-at-AGR.GC.CA Wed Dec 3 14:54:12 2008 10, 21 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3KsB0f024327 10, 21 -- for {Microscopy-at-Microscopy.Com} ; Wed, 3 Dec 2008 14:54:11 -0600 10, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 21 -- Content-class: urn:content-classes:message 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: text/plain; 10, 21 -- charset="iso-8859-1" 10, 21 -- Subject: fluorescence quenching 10, 21 -- Date: Wed, 3 Dec 2008 15:54:11 -0500 10, 21 -- Message-ID: {FD493497DF264A4E8DC71F1FFE49129DD57744-at-ONOTTAXMS2.AGR.GC.CA} 10, 21 -- X-MS-Has-Attach: 10, 21 -- X-MS-TNEF-Correlator: 10, 21 -- Thread-Topic: fluorescence quenching 10, 21 -- Thread-Index: AclViUrkG3JRZihUSDmfu5qf8f2PpA== 10, 21 -- From: "Miller, Shea" {MILLERS-at-AGR.GC.CA} 10, 21 -- To: {Microscopy-at-Microscopy.Com} 10, 21 -- X-OriginalArrivalTime: 03 Dec 2008 20:54:11.0570 (UTC) FILETIME=[4B1AD520:01C95589] 10, 21 -- Content-Transfer-Encoding: 8bit 10, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB3KsB0f024327 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 23 -- From kjmorris-at-well.ox.ac.uk Thu Dec 4 05:57:00 2008 33, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 33, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB4Buxma009252 33, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 4 Dec 2008 05:56:59 -0600 33, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 33, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 33, 23 -- id 1L8CpG-0001yq-P9 33, 23 -- for Microscopy-at-Microscopy.Com; Thu, 04 Dec 2008 11:56:58 +0000 33, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 33, 23 -- To: {Microscopy-at-Microscopy.Com} 33, 23 -- References: {200812032058.mB3KwrAX002457-at-ns.microscopy.com} 33, 23 -- Subject: RE: [Microscopy] fluorescence quenching & autofluorescence 33, 23 -- Date: Thu, 4 Dec 2008 11:56:58 -0000 33, 23 -- Message-ID: {D949726B7784432BB052374747F995DA-at-CytoWhizz} 33, 23 -- MIME-Version: 1.0 33, 23 -- Content-Type: text/plain; 33, 23 -- charset="iso-8859-1" 33, 23 -- X-Mailer: Microsoft Office Outlook 11 33, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 33, 23 -- Thread-Index: AclVifQKyOz/Od3yTninFbDZLqQUogAbYOog 33, 23 -- In-Reply-To: {200812032058.mB3KwrAX002457-at-ns.microscopy.com} 33, 23 -- Content-Transfer-Encoding: 8bit 33, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB4Buxma009252 ==============================End of - Headers==============================
Thank you for the links, they are interesting. BUT I don't have to deal with autofluorescence, neither do I have the option to wash my fluorescent material - it would be wayyyyy to simple. My fluorescent material is not soluble and has a tendency to stick to the cell membrane. Hence the importance to quench extracellular fluorescence. In the mean time I got an information saying that Trypan Blue will leak inside the cells (and be toxic) within 10-15 min, which makes it a bad condidate for live cell imaging. Any idea about quenching still welcome :-)
Stephane
----- Original Message ---- X-from: "kjmorris-at-well.ox.ac.uk" {kjmorris-at-well.ox.ac.uk} To: nizets2-at-yahoo.com Sent: Thursday, December 4, 2008 1:02:06 PM
Hi all,
Generally it is auto-fluorescence in the cells [or tissue] that you wish to quench - you normally don't have loads of fluorochrome fluorescing in the culture/mounting media as such [you replace the media prior to imaging, remove it during washes, and say use a clear CO2 independent culture media for short time-lapse of live cells rather than use the standard red tinted culture media stuff that happily autofluoresces a bit].
We used microscope flow cells and micro-injectors back at UCL, and I don't remember getting serious background fluorescence problems [shame I'm not there now as I'm sure my old Cell Biology colleagues could offer loads of advice, but my job was just to make sure the microscope/flow cell/Eppendorf injectors worked perfectly].
I have used the excellent Wright Cell Imaging Facility's article on auto fluorescence many times and it's linked here [along with some other useful auto fluorescence related stuff]. It discusses your problem.
Except the main link doesn't work, so get it direct from: http://www.uhnresearch.ca/facilities/wcif/PDF/Autofluorescence.pdf
And just search 'auto fluorescence', 'autofluorescence', 'auto-fluorescence', and 'reducing', 'quenching', 'eliminating' and so on......
Although it's not 'autofluorescence' you are trying to remove, the info will still be relevant] - some of it might be aimed at quenching autofluorecence inside the cell though.
Regards
Keith
Note: Amongst other things, Trypan blue is used to quench auto-fluorescence - never actually tried it as generally, say with eye sections/cells, we use the autofluorecence to our advantage and actually don't want it to fade.
With a confocal you can just about image anything that is or has lived using autofluorescence, it's just that generally the autofluorescence brightness is well below the fluorochrome you have added, so you never see it. You simply image unstained cells/tissue as well to check whether autofluorecence was a problem at the imaging gain/laser power or camera exposure you use with the fluorochrome sample].
Some cells are easy to identify owing to their bright autofluorescence, e.g. Autofluorescence in our eye's rods and cones can be used to detect the early onset of eye disease etc..
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: MILLERS-at-AGR.GC.CA [mailto:MILLERS-at-AGR.GC.CA] Sent: 03 December 2008 20:59 To: kjmorris-at-well.ox.ac.uk
Hi;
I have been reading the thread on fluorescence quenching with interest, and wonder if anyone can give a protocol for using trypan blue (all the books say it is minimally soluble in water....)
Thanks in advance
shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1760 Facsimile/Télécopieur: 613-759-1701 Rm 2068 K.W. Neatby Bldg 960 Carling Ave. Central Experimental Farm Ottawa, ON K1A 0C6 millers-at-agr.gc.ca
==============================Original Headers============================== 10, 21 -- From MILLERS-at-AGR.GC.CA Wed Dec 3 14:54:12 2008 10, 21 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB3KsB0f024327 10, 21 -- for {Microscopy-at-Microscopy.Com} ; Wed, 3 Dec 2008 14:54:11 -0600 10, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 21 -- Content-class: urn:content-classes:message 10, 21 -- MIME-Version: 1.0 10, 21 -- Content-Type: text/plain; 10, 21 -- charset="iso-8859-1" 10, 21 -- Subject: fluorescence quenching 10, 21 -- Date: Wed, 3 Dec 2008 15:54:11 -0500 10, 21 -- Message-ID: {FD493497DF264A4E8DC71F1FFE49129DD57744-at-ONOTTAXMS2.AGR.GC.CA} 10, 21 -- X-MS-Has-Attach: 10, 21 -- X-MS-TNEF-Correlator: 10, 21 -- Thread-Topic: fluorescence quenching 10, 21 -- Thread-Index: AclViUrkG3JRZihUSDmfu5qf8f2PpA== 10, 21 -- From: "Miller, Shea" {MILLERS-at-AGR.GC.CA} 10, 21 -- To: {Microscopy-at-Microscopy.Com} 10, 21 -- X-OriginalArrivalTime: 03 Dec 2008 20:54:11.0570 (UTC) FILETIME=[4B1AD520:01C95589] 10, 21 -- Content-Transfer-Encoding: 8bit 10, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB3KsB0f024327 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 23 -- From kjmorris-at-well.ox.ac.uk Thu Dec 4 05:57:00 2008 33, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 33, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB4Buxma009252 33, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 4 Dec 2008 05:56:59 -0600 33, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 33, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 33, 23 -- id 1L8CpG-0001yq-P9 33, 23 -- for Microscopy-at-Microscopy.Com; Thu, 04 Dec 2008 11:56:58 +0000 33, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 33, 23 -- To: {Microscopy-at-Microscopy.Com} 33, 23 -- References: {200812032058.mB3KwrAX002457-at-ns.microscopy.com} 33, 23 -- Subject: RE: [Microscopy] fluorescence quenching & autofluorescence 33, 23 -- Date: Thu, 4 Dec 2008 11:56:58 -0000 33, 23 -- Message-ID: {D949726B7784432BB052374747F995DA-at-CytoWhizz} 33, 23 -- MIME-Version: 1.0 33, 23 -- Content-Type: text/plain; 33, 23 -- charset="iso-8859-1" 33, 23 -- X-Mailer: Microsoft Office Outlook 11 33, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 33, 23 -- Thread-Index: AclVifQKyOz/Od3yTninFbDZLqQUogAbYOog 33, 23 -- In-Reply-To: {200812032058.mB3KwrAX002457-at-ns.microscopy.com} 33, 23 -- Content-Transfer-Encoding: 8bit 33, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB4Buxma009252 ==============================End of - Headers==============================
==============================Original Headers============================== 50, 23 -- From nizets2-at-yahoo.com Thu Dec 4 06:55:13 2008 50, 23 -- Received: from web110810.mail.gq1.yahoo.com (web110810.mail.gq1.yahoo.com [67.195.13.233]) 50, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB4CtDFi024486 50, 23 -- for {microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 06:55:13 -0600 50, 23 -- Received: (qmail 55960 invoked by uid 60001); 4 Dec 2008 12:55:12 -0000 50, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 50, 23 -- s=s1024; d=yahoo.com; 50, 23 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 50, 23 -- b=3h2pFmnsbSo8RAhpJsSdEZVA/BNV0renC2JK19ve9NftMXFGXdRGszp52h1r6QF21fhOpoCW/IfnlrXzfrZEv6HRWS1xPPEREVIndZ5wraS16Jq29+6QMPa/quxin4x3QrZEC9gHryalvq4it8hw6V1XoSTqY6VStEOJZecLCjk=; 50, 23 -- X-YMail-OSG: gn_GMPwVM1lA61XuecZZuyxX.2xMnUnpQqZhQdxmi8p685TEdlwSC.XyNxaCe_tS5v_XVfQ8bP.JNu_eAr5AbwKUbO5I.yrIk2IIDw9qFL5juocTzi1tzp8MkgqOU_D.C3N8DTz_zwJXp0AneCfjLsWNej21PqSZV389cixZF1kpS0jHOk1X3gPhx9B_Jdi_RoV61.OTFN0CiLKamF2yg_jmwcoyUmLwNjiTNUhoUgDvuWWM 50, 23 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Thu, 04 Dec 2008 04:55:12 PST 50, 23 -- X-Mailer: YahooMailRC/1155.32 YahooMailWebService/0.7.260.1 50, 23 -- References: {200812041202.mB4C26c1015931-at-ns.microscopy.com} 50, 23 -- Date: Thu, 4 Dec 2008 04:55:12 -0800 (PST) 50, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com} 50, 23 -- Subject: Re: [Microscopy] RE: fluorescence quenching & autofluorescence 50, 23 -- To: kjmorris-at-well.ox.ac.uk 50, 23 -- Cc: microscopy-at-microscopy.com 50, 23 -- MIME-Version: 1.0 50, 23 -- Content-Type: text/plain; charset=iso-8859-1 50, 23 -- Message-ID: {854885.55826.qm-at-web110810.mail.gq1.yahoo.com} 50, 23 -- Content-Transfer-Encoding: 8bit 50, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB4CtDFi024486 ==============================End of - Headers==============================
We're currently investigating new TEMs. So CCD cameras are also an issue.
The TEM we're looking for will be your standard biological TEM used for kidneys and tumours, etc. 60 - 100kV.
Some of the cameras appear to be beyond our specs, ie, they operate above 120kV.
Are there any cameras that are not compatible with particular TEMs? Conversely are there any cameras that perform better with a particular model of TEM?
Any advice or comments on this issue greatly appreciated since it's hard to compare cameras off a brochure without the experience of having used one.
Regards,
John Brealey EM Unit Queen Elizabeth Hospital South Australia
==============================Original Headers============================== 9, 35 -- From john.brealey-at-imvs.sa.gov.au Thu Dec 4 18:00:33 2008 9, 35 -- Received: from mailgate8.sa.gov.au (mailgate8.sa.gov.au [203.26.121.13]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB500VSD025523 9, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 18:00:32 -0600 9, 35 -- X-IronPort-AV: E=Sophos;i="4.33,717,1220193000"; 9, 35 -- d="scan'208";a="6104016" 9, 35 -- Received: from unknown (HELO EMSGM301.sagemsmrd01.sa.gov.au) ([10.9.18.88]) 9, 35 -- by mailgate8.sa.gov.au with ESMTP; 05 Dec 2008 10:30:29 +1030 9, 35 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 9, 35 -- EMSGM301.sagemsmrd01.sa.gov.au (10.9.18.88) with Microsoft SMTP Server id 9, 35 -- 8.1.263.0; Fri, 5 Dec 2008 10:30:29 +1030 9, 35 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) by 9, 35 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id A55EB34D04 for 9, 35 -- {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 10:30:05 +1030 (CST) 9, 35 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 9, 35 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) by localhost 9, 35 -- (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) with LMTP id 9, 35 -- dHfk7tJ7ziwm for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 10:29:53 +1030 9, 35 -- (CST) 9, 35 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 9, 35 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id D651634CF6 for 9, 35 -- {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 10:29:43 +1030 (CST) 9, 35 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 9, 35 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 9, 35 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 35 -- Subject: CCD Cameras 9, 35 -- Date: Fri, 5 Dec 2008 10:29:49 +1030 9, 35 -- Organization: IMVS 9, 35 -- Message-ID: {000001c9566c$64814560$7d8a140a-at-41347i} 9, 35 -- MIME-Version: 1.0 9, 35 -- Content-Type: text/plain; charset="us-ascii" 9, 35 -- Content-Transfer-Encoding: 7bit 9, 35 -- X-Mailer: Microsoft Office Outlook 11 9, 35 -- Thread-Index: AclWbGQTZEoLV7fCQU+LfoKkWda6CQ== 9, 35 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gw265-at-cam.ac.uk Name: Giselle Walker
Organization: University of Cambridge
Title-Subject: [Filtered] Heat-pen vs Chloroform for straightening sections
Question: Some weeks back I asked the list about alternatives to chloroform for straightening out thin resin sections for TEM.
After an overwhelming response of "HEAT PEN!!!!!" I duly got one, sold by Fine Science Tools. The tip supposedly heats up to 1000 degrees, and is certainly hot enough to go white and deliver a good burn to skin. {http://www.finescience.ca/commerce/ccp4151-low-cost-cautery-kit-18010-00m.htm}
However I'm finding that it's nowhere near as effective as chloroform: my sections are still too wrinkled to use, unless I use chloroform as well. The sections are 30, 40 or 50nm thick and of single protistan cells in Spurr's resin.
I've tried holding the heated tip as close as possible to the sections, a few at a time. All that happens is that the ribbon shoots away (as a whole) over the surface of the water bath. There's no discernible difference between holding the tip 5m, 5cm, or 5mm away from the sections.
Clearly I'm doing something wrong. Ideas would be very welcome!
I suppose the next alternative to chloroform is wet-resin embedding for 3 weeks before polymerisation, which I haven't tried yet. Given that this isn't always feasible, it would be good to get the heat-pen technique working.
I have used the heat pen on Epon sections but have not tried it on Spurr's. It worked about the same as Cholorform.
Sorry but I do not remember if there was a specific reason for using Spurr's.
Some ways of reducing section wrinkle problems are to make the sections narrower and make sure that you do not cut through blank resin before hitting the tissue of interest. This does not work all the time but I have found that it does improve my results to the point that I do not need to stretch sections all the time.
Pat Connelly NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {gw265-at-cam.ac.uk} } Reply-To: {gw265-at-cam.ac.uk} } Date: Thu, 4 Dec 2008 18:14:57 -0600 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] viaWWW: Heat-pen vs Chloroform for straightening } sections ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: gw265-at-cam.ac.uk } Name: Giselle Walker } } Organization: University of Cambridge } } Title-Subject: [Filtered] Heat-pen vs Chloroform for straightening sections } } Question: Some weeks back I asked the list about alternatives to } chloroform for straightening out thin resin sections for TEM. } } After an overwhelming response of "HEAT PEN!!!!!" I duly got one, } sold by Fine Science Tools. The tip supposedly heats up to 1000 } degrees, and is certainly hot enough to go white and deliver a good } burn to skin. } {http://www.finescience.ca/commerce/ccp4151-low-cost-cautery-kit-18010-00m.htm} } } } However I'm finding that it's nowhere near as effective as } chloroform: my sections are still too wrinkled to use, unless I use } chloroform as well. The sections are 30, 40 or 50nm thick and of } single protistan cells in Spurr's resin. } } I've tried holding the heated tip as close as possible to the } sections, a few at a time. All that happens is that the ribbon shoots } away (as a whole) over the surface of the water bath. There's no } discernible difference between holding the tip 5m, 5cm, or 5mm away } from the sections. } } Clearly I'm doing something wrong. Ideas would be very welcome! } } I suppose the next alternative to chloroform is wet-resin embedding } for 3 weeks before polymerisation, which I haven't tried yet. Given } that this isn't always feasible, it would be good to get the heat-pen } technique working. } } Thanks, } } Giselle
==============================Original Headers============================== 10, 28 -- From connellyps-at-nhlbi.nih.gov Thu Dec 4 19:22:30 2008 10, 28 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB51MUUS022064 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 19:22:30 -0600 10, 28 -- X-IronPortListener: Outbound_SMTP 10, 28 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 10, 28 -- by nihxwayout.hub.nih.gov with ESMTP; 04 Dec 2008 19:53:20 -0500 10, 28 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 10, 28 -- Thu, 4 Dec 2008 19:53:15 -0500 10, 28 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.160]) with Microsoft Exchange Server HTTP-DAV ; 10, 28 -- Fri, 5 Dec 2008 00:53:15 +0000 10, 28 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 28 -- Date: Thu, 04 Dec 2008 19:52:47 -0500 10, 28 -- Subject: Re: [Microscopy] viaWWW: Heat-pen vs Chloroform for straightening 10, 28 -- sections 10, 28 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 28 -- To: {gw265-at-cam.ac.uk} , "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 28 -- Message-ID: {C55DE58F.2A91%connellyps-at-nhlbi.nih.gov} 10, 28 -- Thread-Topic: [Microscopy] viaWWW: Heat-pen vs Chloroform for 10, 28 -- straightening sections 10, 28 -- Thread-Index: AclWc8otCKylJMJnEd2irQANk2Yv1A== 10, 28 -- In-Reply-To: {200812050014.mB50EvAK014712-at-ns.microscopy.com} 10, 28 -- Mime-version: 1.0 10, 28 -- Content-type: text/plain; 10, 28 -- charset="ISO-8859-1" 10, 28 -- X-OriginalArrivalTime: 05 Dec 2008 00:53:15.0555 (UTC) FILETIME=[DB32FF30:01C95673] 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB51MUUS022064 ==============================End of - Headers==============================
The way I use our heat pen is to pick up the sections (floating on a large water droplet) on naked grids and pass the grid between the heated loops of our heat pen. Our heat pen does not get as hot as yours, so there is no danger of vaporizing the water/sections as with your pen. However, you might try picking up sections so that they are floating on a large droplet on the grid and slowly approach your pen until you see them relax. Observe the relaxation under a stereomicroscope, if possible. Also, do this using expendible sections, until you figure out if this will work reliably for you.
If you try to relax the sections as they float in the water trough of the knife, they will run away from the heat. You need to confine them (either on the grid, or a loop containing the sections). If you have problems with the grid method, then use a wire loop (like the EMS Perfect Loop) to warm the sections and then bring down the loop over your grids so the sections transfer.
Let us know how you make out.
JB
} However I'm finding that it's nowhere near as effective as } chloroform: my sections are still too wrinkled to use, unless I use } chloroform as well. The sections are 30, 40 or 50nm thick and of } single protistan cells in Spurr's resin. } } I've tried holding the heated tip as close as possible to the } sections, a few at a time. All that happens is that the ribbon shoots } away (as a whole) over the surface of the water bath. There's no } discernible difference between holding the tip 5m, 5cm, or 5mm away } from the sections. } } Clearly I'm doing something wrong. Ideas would be very welcome!
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I have had the same experience that you describe. I have a homemade heat pen and don't find it very effective when held over the sections in the knife trough; and I get the same wild section movements and almost no flattening.
But there is a picture of sections in a drop of water held on a grid that is placed through the center of a heater hairpin loop - this is in the Bozzola and Russell book (Electron Microscopy - Principles and Techniques for Biologists (Fig 4-44b of 1992 1st edition)); this is a different style of heater loop than you have purchased; it is longer and more open. I have not yet tried this because I don't pick up sections that way in general, but wonder if it wouldn't be more effective since they get heated from both sides in a much smaller volume of water; I think that the sections on the trough surface are never very hot due to the large volume of water below and all the motion.
Cheers,
Dale
gw265-at-cam.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both gw265-at-cam.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: gw265-at-cam.ac.uk } Name: Giselle Walker } } Organization: University of Cambridge } } Title-Subject: [Filtered] Heat-pen vs Chloroform for straightening sections } } Question: Some weeks back I asked the list about alternatives to } chloroform for straightening out thin resin sections for TEM. } } After an overwhelming response of "HEAT PEN!!!!!" I duly got one, } sold by Fine Science Tools. The tip supposedly heats up to 1000 } degrees, and is certainly hot enough to go white and deliver a good } burn to skin. } {http://www.finescience.ca/commerce/ccp4151-low-cost-cautery-kit-18010-00m.htm} } } However I'm finding that it's nowhere near as effective as } chloroform: my sections are still too wrinkled to use, unless I use } chloroform as well. The sections are 30, 40 or 50nm thick and of } single protistan cells in Spurr's resin. } } I've tried holding the heated tip as close as possible to the } sections, a few at a time. All that happens is that the ribbon shoots } away (as a whole) over the surface of the water bath. There's no } discernible difference between holding the tip 5m, 5cm, or 5mm away } from the sections. } } Clearly I'm doing something wrong. Ideas would be very welcome! } } I suppose the next alternative to chloroform is wet-resin embedding } for 3 weeks before polymerisation, which I haven't tried yet. Given } that this isn't always feasible, it would be good to get the heat-pen } technique working. } } } Thanks, } } Giselle } } Login Host: 131.111.8.96 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 14, 11 -- From zaluzec-at-microscopy.com Thu Dec 4 18:12:03 2008 } 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB50C2hB007160 } 14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 18:12:02 -0600 } 14, 11 -- Mime-Version: 1.0 } 14, 11 -- Message-Id: {p06240800c55e2242c536-at-[206.69.208.22]} } 14, 11 -- Date: Thu, 4 Dec 2008 18:12:01 -0600 } 14, 11 -- To: microscopy-at-microscopy.com } 14, 11 -- From: gw265-at-cam.ac.uk (by way of MicroscopyListserver) } 14, 11 -- Subject: viaWWW: Heat-pen vs Chloroform for straightening sections } 14, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 21 -- From dac-at-research.umass.edu Thu Dec 4 19:42:14 2008 6, 21 -- Received: from race4.oit.umass.edu (race4.oit.umass.edu [128.119.101.40]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB51gEMA016716 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 19:42:14 -0600 6, 21 -- Received: from [192.168.1.101] (static.unknown.charter.com [96.39.6.64] (may be forged)) 6, 21 -- (authenticated bits=0) 6, 21 -- by race4.oit.umass.edu (8.14.3/8.14.3) with ESMTP id mB51g8Dx030092 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 6, 21 -- Thu, 4 Dec 2008 20:42:10 -0500 6, 21 -- Message-ID: {4938870B.6090302-at-research.umass.edu} 6, 21 -- Date: Thu, 04 Dec 2008 20:42:35 -0500 6, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.18) Gecko/20081031 SeaMonkey/1.1.13 6, 21 -- MIME-Version: 1.0 6, 21 -- To: gw265-at-cam.ac.uk, Microscopy List {microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] viaWWW: Heat-pen vs Chloroform for straightening 6, 21 -- sections 6, 21 -- References: {200812050015.mB50FCK2015379-at-ns.microscopy.com} 6, 21 -- In-Reply-To: {200812050015.mB50FCK2015379-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I noticed the same thing, a while ago. Whenever I need to get the sections stretched, Heat Pen does not come close to what chloroform does. Well, I guess, that is to say that chloroform always restores the round shape to what is supposed to be round, while heat pen most often does not, in my clumsy hands.
What is "wet-resin embedding"?..
May I suggest a deeper search of this server's archives - back to late Hildy Crowley's posts? With her experience and knowledge of resin chemistry, she was making a point that if you have to stretch you sections after cutting, than you are not embedding right. Sorry, I know, this is not a particularly constructive remark, but something to keep in mind. There is more constructive (well, instructive at least :)) detail in those posts there.
Spurr seems like the resin that I used to have to stretch more often, although I am not a 100% sure. I vividly remember though those Spurr- embedded yeast, ellipsoid w/o chloroform. The hypothesis of Spurr being more compression-prone sounds reasonable, doesn't it, if you consider that Spurr blocks are the most cross-linked of all resins?
Finally, there is very often just a little compression in freshly cut epoxy sections that goes away if you just leave them float for a few minutes, keeping the light on. There would be a notable color change.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
} Email: gw265-at-cam.ac.uk } Name: Giselle Walker } } Organization: University of Cambridge } } Title-Subject: [Filtered] Heat-pen vs Chloroform for straightening } sections } } Question: Some weeks back I asked the list about alternatives to } chloroform for straightening out thin resin sections for TEM. } } After an overwhelming response of "HEAT PEN!!!!!" I duly got one, } sold by Fine Science Tools. The tip supposedly heats up to 1000 } degrees, and is certainly hot enough to go white and deliver a good } burn to skin. } {http://www.finescience.ca/commerce/ccp4151-low-cost-cautery-kit-18010-00m.htm } } } } However I'm finding that it's nowhere near as effective as } chloroform: my sections are still too wrinkled to use, unless I use } chloroform as well. The sections are 30, 40 or 50nm thick and of } single protistan cells in Spurr's resin. } } I've tried holding the heated tip as close as possible to the } sections, a few at a time. All that happens is that the ribbon shoots } away (as a whole) over the surface of the water bath. There's no } discernible difference between holding the tip 5m, 5cm, or 5mm away } from the sections. } } Clearly I'm doing something wrong. Ideas would be very welcome! } } I suppose the next alternative to chloroform is wet-resin embedding } for 3 weeks before polymerisation, which I haven't tried yet. Given } that this isn't always feasible, it would be good to get the heat-pen } technique working. } } } Thanks, } } Giselle } } Login Host: 131.111.8.96 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 14, 11 -- From zaluzec-at-microscopy.com Thu Dec 4 18:12:03 2008 } 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 14, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id mB50C2hB007160 } 14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 } 18:12:02 -0600 } 14, 11 -- Mime-Version: 1.0 } 14, 11 -- Message-Id: {p06240800c55e2242c536-at-[206.69.208.22]} } 14, 11 -- Date: Thu, 4 Dec 2008 18:12:01 -0600 } 14, 11 -- To: microscopy-at-microscopy.com } 14, 11 -- From: gw265-at-cam.ac.uk (by way of MicroscopyListserver) } 14, 11 -- Subject: viaWWW: Heat-pen vs Chloroform for straightening } sections } 14, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 12, 23 -- From vladislav_speransky-at-nih.gov Thu Dec 4 19:43:49 2008 12, 23 -- Received: from nihrelayxway.hub.nih.gov (mailfwd.nih.gov [128.231.90.106]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB51hmMZ020490 12, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 19:43:49 -0600 12, 23 -- X-IronPortListener: NIH_Relay 12, 23 -- X-SBRS: None 12, 23 -- X-IronPort-AV: E=Sophos;i="4.33,717,1220241600"; 12, 23 -- d="scan'208";a="76862282" 12, 23 -- Received: from helix.nih.gov ([128.231.2.3]) 12, 23 -- by nihrelayxway.hub.nih.gov with ESMTP; 04 Dec 2008 20:43:48 -0500 12, 23 -- Received: from db447.niaid.nih.gov (db447.niaid.nih.gov [128.231.217.47]) 12, 23 -- by helix.nih.gov (8.13.8/8.13.8) with ESMTP id mB51hiQh026253 12, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 20:43:47 -0500 12, 23 -- Message-Id: {22ADA5CD-1861-4BA0-A71E-DEC19897AB76-at-nih.gov} 12, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 12, 23 -- To: Microscopy-at-microscopy.com 12, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- Mime-Version: 1.0 (Apple Message framework v929.2) 12, 23 -- Subject: Fwd: [Microscopy] viaWWW: Heat-pen vs Chloroform for straightening sections 12, 23 -- Date: Thu, 4 Dec 2008 20:43:43 -0500 12, 23 -- References: {200812050012.mB50CepQ008515-at-ns.microscopy.com} 12, 23 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I suppose I should make comment, as I did to your first enquiry.
I have used Spurr's for many years and had few problems with flattening silver/gold (80-100nm) sections on an ultramicrotome cutting at 1-2mm/sec speed. I have always used a medium/hard mix of Spurr's with few problems unless the embedding has been soft.
I can think of several possible sources of the problem: The heat pen that I use is a tungsten wire type which glows red/yellow but certainly not white. Mine uses a fixed mains electric controller, but some used to be available with a variable heat adjustment. Another possible source of problems is the change of formulation of Spurr's resin over the last year or so. It would be worth you trying some much older Spurr's blocks if you are using the new Spurr's. A final issue that sometimes arises in some environments is the build up of static on the surface of sections which tends to make the sections disperse when you approach with a heat pen or tweezers and grid sometimes. Humidifiers or anti-static guns may solve this but it would be important to establish that this is the problem before spending more money.
I hope this is of some help and will keep you away from the dreaded chloroform.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: gw265-at-cam.ac.uk
Microanalysis Applications Engineer - EBSD and EDS
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---------------------------------------------------------------------- Doug Skinner Assistant Vice President, Microanalysis Bruker AXS Inc. 1239 Parkway Ave. Suite 203 Ewing, NJ 08628 USA Tel: +1 (609) 771-4427 Fax: +1 (609) 771-4411 doug.skinner-at-bruker-axs.com www.bruker-axs.com ----------------------------------------------------------------------
==============================Original Headers============================== 14, 28 -- From Doug.Skinner-at-bruker-axs.com Fri Dec 5 14:24:38 2008 14, 28 -- Received: from mail1.bruker-axs.com (mail1.bruker-axs.com [68.22.68.158]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB5KOb3V025863 14, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 14:24:38 -0600 14, 28 -- Received: from mail1.bruker-axs.com ([172.16.0.32]) by mail1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.3959); 14, 28 -- Fri, 5 Dec 2008 14:27:50 -0600 14, 28 -- X-Ninja-PIM: Scanned by Ninja 14, 28 -- X-Ninja-AttachmentFiltering: (no action) 14, 28 -- Received: from msnmail1.bruker-axs.com ([172.16.0.53]) by mail1.bruker-axs.com with Microsoft SMTPSVC(6.0.3790.3959); 14, 28 -- Fri, 5 Dec 2008 14:24:36 -0600 14, 28 -- Received: from msnmail1.bruker-axs.com ([172.16.0.53]) by 14, 28 -- msnmail1.bruker-axs.com ([172.16.0.53]) with mapi; Fri, 5 Dec 2008 14:24:36 14, 28 -- -0600 14, 28 -- From: "Skinner, Doug" {Doug.Skinner-at-bruker-axs.com} 14, 28 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 14, 28 -- Date: Fri, 5 Dec 2008 14:24:36 -0600 14, 28 -- Subject: Job Opportunity at Bruker AXS Microanalysis 14, 28 -- Thread-Topic: Job Opportunity at Bruker AXS Microanalysis 14, 28 -- Thread-Index: AclXF311l4sQPtmfSm25Q7od+rKZ3Q== 14, 28 -- Message-ID: {6CBB09E530A398488E07976781F692030EC1E4B0F2-at-msnmail1.bruker-axs.com} 14, 28 -- Accept-Language: en-US 14, 28 -- Content-Language: en-US 14, 28 -- acceptlanguage: en-US 14, 28 -- Content-Type: text/plain; charset="us-ascii" 14, 28 -- MIME-Version: 1.0 14, 28 -- X-OriginalArrivalTime: 05 Dec 2008 20:24:36.0614 (UTC) FILETIME=[7DF98A60:01C95717] 14, 28 -- Content-Transfer-Encoding: 8bit 14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB5KOb3V025863 ==============================End of - Headers==============================
Many years ago, I discovered a style of frames called "Focal Change".
They had a cleverly designed nosepiece that could be shifted up or down, raising or lowering the lenses. As a result, I can adjust my trifocal glasses for looking at the monitor, or for reading paper. They are also wonderful for allowing me to look at small etchings or drawings in the museum, where I have to get close to see details.
The downsides: They are no longer made. I was able to find one pair on e-bay earlier this year. Also, they are designed for large lenses, so I have a continuing debate with my wife --no good solution, except that I have a pair of "social event" glasses as well as my normal ones.
Ah the price we pay for style!!
Joel
Previously,.......... } Owen; } Get a motorcycle to be cool. Don't let your wife design your } glasses unless she is an optician. A lot depends on the nature of the } correction you have. If you have severe presbyopia, you should get two } prescriptions, one for close up and one for driving. When you try to } accommodate too wide a range of focal lengths in progressives or bi/tri } focals, the focal length gradients make looking through the lenses very } difficult. Interestingly, every eye doctor I have ever been to did not } know how to do this-I had to tell him what I wanted. If you have tiny } lenses, the focal length gradient will be more severe no matter what. } Besides, with the larger computer monitors common today, you will need a } wider field of view. I also strongly recommend nitinol frames. Opticians } call them titanium frames, but they are a TiNi alloy called nitinol, } which is also well known as a shape memory alloy. Not only are these } frames lighter and nearly indestructible, but they are extremely } corrosion resistant, and are especially immune from salty perspiration } running all over them. They do cost twice as much a regular frames, but } the ones I am wearing right now are something like 15 years old, look } new, and I have no plans on replacing them. } } John Mardinly, } Numonyx } -- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 14, 40 -- From joelsheffield-at-gmail.com Fri Dec 5 16:13:19 2008 14, 40 -- Received: from qw-out-1920.google.com (qw-out-1920.google.com [74.125.92.145]) 14, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB5MDJNF011995 14, 40 -- for {microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 16:13:19 -0600 14, 40 -- Received: by qw-out-1920.google.com with SMTP id 4so68192qwk.54 14, 40 -- for {microscopy-at-microscopy.com} ; Fri, 05 Dec 2008 14:13:19 -0800 (PST) 14, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 40 -- d=gmail.com; s=gamma; 14, 40 -- h=domainkey-signature:received:received:from:to:date:mime-version 14, 40 -- :subject:message-id:priority:x-mailer:content-type 14, 40 -- :content-transfer-encoding:content-description; 14, 40 -- bh=Ml6BcTOSYsX33LvgDqs4qY0WKctRb8KKtGtL5VY76mA=; 14, 40 -- b=PfmmbiitIWpxkdSZYm5xMAjbj6xvO17q3I9qceWKoKrHztrByYT5i/pPmQ0ToaTkSl 14, 40 -- xagpWvMlmXjBmGIhiA1Bpx8Yhhibpi8uRLLqh//SXaryNkNVlXAchMMiRQ26PtG8BkQ8 14, 40 -- CDxokGjAmnjovmanptBMJgdbJAEzXwsiMNqj8= 14, 40 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 14, 40 -- d=gmail.com; s=gamma; 14, 40 -- h=from:to:date:mime-version:subject:message-id:priority:x-mailer 14, 40 -- :content-type:content-transfer-encoding:content-description; 14, 40 -- b=WYS9x9+afv+XxD/yjtZOsGnxrmCfPoeWR60p0MxBVBk7SwUvxw2sqc6BF7QyE0jCYo 14, 40 -- UPiEVs27egJBNpoeOtr9Gt8CV4Pnnj9qbcC3OFA76RSDfrF6w4duL+vrcptfiVqO8oJP 14, 40 -- m3Bi3jiy0nvZocHTwuamrYCFt3PLJvctCXEcE= 14, 40 -- Received: by 10.214.184.3 with SMTP id h3mr849414qaf.179.1228515199115; 14, 40 -- Fri, 05 Dec 2008 14:13:19 -0800 (PST) 14, 40 -- Received: from ?155.247.98.40? (jbs.bio.temple.edu [155.247.98.40]) 14, 40 -- by mx.google.com with ESMTPS id 4sm11437954yxq.9.2008.12.05.14.13.18 14, 40 -- (version=SSLv3 cipher=RC4-MD5); 14, 40 -- Fri, 05 Dec 2008 14:13:18 -0800 (PST) 14, 40 -- From: joelsheffield-at-gmail.com 14, 40 -- To: microscopy-at-microscopy.com 14, 40 -- Date: Fri, 05 Dec 2008 17:13:20 -0500 14, 40 -- MIME-Version: 1.0 14, 40 -- Subject: re: eye glasses 14, 40 -- Message-ID: {49396130.26178.305A7689-at-joelsheffield.gmail.com} 14, 40 -- Priority: normal 14, 40 -- X-mailer: Pegasus Mail for Windows (4.41) 14, 40 -- Content-type: text/plain; charset=ISO-8859-1 14, 40 -- Content-description: Mail message body 14, 40 -- Content-Transfer-Encoding: 8bit 14, 40 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id mB5MDJNF011995 ==============================End of - Headers==============================
We are starting a new FIB Lab and having some acoustic issues. I would like to get recommendations on what kind of acoustic material/product is the best for a FIB or a TEM Lab? Would someone like to share personal experiences, good or bad?? And/or recommend particular product/s and vendors??
Thanks.
Zia ur Rahman FIB Laboratory Manager, NASA Johnson Space Center, Houston, TX Email: Zia.rahman-1-at-nasa.gov
==============================Original Headers============================== 4, 29 -- From Zia.Rahman-1-at-nasa.gov Fri Dec 5 18:32:02 2008 4, 29 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov [198.117.0.123]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB60W2Ls028620 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:02 -0600 4, 29 -- Received: from ndjsppt03.ndc.nasa.gov (ndjsppt03.ndc.nasa.gov [198.117.1.102]) 4, 29 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id 797182D801C 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 -0600 (CST) 4, 29 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) 4, 29 -- by ndjsppt03.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id mB60W1n9016251 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 -0600 4, 29 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 4, 29 -- Fri, 5 Dec 2008 18:32:01 -0600 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 4, 29 -- Content-class: urn:content-classes:message 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; 4, 29 -- charset="us-ascii" 4, 29 -- Subject: FIB/TEM Acoustic Damping in Labs. 4, 29 -- Date: Fri, 5 Dec 2008 18:32:00 -0600 4, 29 -- Message-ID: {B385F8289FD0B144A75B3A8C3E434E2F2651B1-at-NDJSEVS25A.ndc.nasa.gov} 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- Thread-Topic: FIB/TEM Acoustic Damping in Labs. 4, 29 -- Thread-Index: AclXOg2EjoVzYyhPQrag5AMgoVOGug== 4, 29 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- X-OriginalArrivalTime: 06 Dec 2008 00:32:01.0303 (UTC) FILETIME=[0E195270:01C9573A] 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB60W2Ls028620 ==============================End of - Headers==============================
You a lucky if this only an acoustic problem and is not accompanied by floor vibrations.
As to noise insulation - I can't be sure if my DIY solution will be suitable for your situation, but little more then two years ago I was setting a SEM/FIB lab in industrial location and had to deal with excessive noise - woodworking shop on other side of the building, but separated by only a drywall from me. Specialized acoustic foams were out of question for cost reasons, so out of necessity I devised noise-dampening panels, made from two layers of heat-insulating foam from Home Depot and.... sandwiched between the layers of foam 12" x 12" egg trays made from recycled pulp (paper), which were obtained free of charge from one of local businesses. "Liquid Nails" construction glue was used to hold everything together, and the result was... well, deafening :) I did not conduct any acoustic measurements, but when the offending wall was covered by these home-made panels (same "Liquid Nails" glue for mounting to drywall) previously loud disk saws running across the wall could be barely heard, and that is only when all my switched off; fans of three PCs were about as loud as the outside noise - good enough for my purposes.
If anyone wants more details I can look up which foam was used and send detailed explanation on how panels were sandwiched together and finished. I believe that for compliance with building codes paper egg cartons must be sprayed with fire retardant before they allowed in construction, though I did not worry about it at the time.
Cheers, Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: Zia.Rahman-1-at-nasa.gov [mailto:Zia.Rahman-1-at-nasa.gov] Sent: Friday, December 05, 2008 7:34 PM To: vray-at-partbeamsystech.com
We are starting a new FIB Lab and having some acoustic issues. I would like to get recommendations on what kind of acoustic material/product is the best for a FIB or a TEM Lab? Would someone like to share personal experiences, good or bad?? And/or recommend particular product/s and vendors??
Thanks.
Zia ur Rahman FIB Laboratory Manager, NASA Johnson Space Center, Houston, TX Email: Zia.rahman-1-at-nasa.gov
==============================Original Headers============================== 4, 29 -- From Zia.Rahman-1-at-nasa.gov Fri Dec 5 18:32:02 2008 4, 29 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov [198.117.0.123]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB60W2Ls028620 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:02 -0600 4, 29 -- Received: from ndjsppt03.ndc.nasa.gov (ndjsppt03.ndc.nasa.gov [198.117.1.102]) 4, 29 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id 797182D801C 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 -0600 (CST) 4, 29 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov [129.166.32.111]) 4, 29 -- by ndjsppt03.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id mB60W1n9016251 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 -0600 4, 29 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 4, 29 -- Fri, 5 Dec 2008 18:32:01 -0600 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 4, 29 -- Content-class: urn:content-classes:message 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; 4, 29 -- charset="us-ascii" 4, 29 -- Subject: FIB/TEM Acoustic Damping in Labs. 4, 29 -- Date: Fri, 5 Dec 2008 18:32:00 -0600 4, 29 -- Message-ID: {B385F8289FD0B144A75B3A8C3E434E2F2651B1-at-NDJSEVS25A.ndc.nasa.gov} 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- Thread-Topic: FIB/TEM Acoustic Damping in Labs. 4, 29 -- Thread-Index: AclXOg2EjoVzYyhPQrag5AMgoVOGug== 4, 29 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- X-OriginalArrivalTime: 06 Dec 2008 00:32:01.0303 (UTC) FILETIME=[0E195270:01C9573A] 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB60W2Ls028620 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 25 -- From vray-at-partbeamsystech.com Sat Dec 6 00:17:38 2008 16, 25 -- Received: from smtp100.biz.mail.re2.yahoo.com (smtp100.biz.mail.re2.yahoo.com [206.190.52.46]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB66HbAZ015447 16, 25 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 00:17:38 -0600 16, 25 -- Message-Id: {200812060617.mB66HbAZ015447-at-ns.microscopy.com} 16, 25 -- Received: (qmail 40967 invoked from network); 6 Dec 2008 06:17:37 -0000 16, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) 16, 25 -- by smtp100.biz.mail.re2.yahoo.com with SMTP; 6 Dec 2008 06:17:37 -0000 16, 25 -- X-YMail-OSG: g5J6aRgVM1lF8lJ1.Zsv.MxUmfZBk2i79eug8g2G.wWT4bBOFWrnh1ytcOMTdqVd.xnxKftupnUg29l66hlkq4mVFabVR3fsqZE.LpEiOrB6EcBsx6gZALjTkQtL_NtVvB8DI7zyM8xQEa3LFBEg2q9Axzg8_RVKrRBmyUo1kx.4T4XfjFpHPjvqvvjYH_u9j7_I5qhXX5VQYfSBicrSBuS1AQLeaWnEmkzI5cnLubdrgUa9ylh6PDhYvkIa 16, 25 -- X-Yahoo-Newman-Property: ymail-3 16, 25 -- Reply-To: {vray-at-partbeamsystech.com} 16, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 16, 25 -- To: {Zia.Rahman-1-at-nasa.gov} 16, 25 -- Cc: {microscopy-at-microscopy.com} 16, 25 -- Subject: RE: [Microscopy] FIB/TEM Acoustic Damping in Labs. 16, 25 -- Date: Sat, 6 Dec 2008 01:20:15 -0500 16, 25 -- Organization: PBST / MEO Engineering 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Type: text/plain; 16, 25 -- charset="us-ascii" 16, 25 -- Content-Transfer-Encoding: 7bit 16, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 16, 25 -- Thread-Index: AclXOk7jU8z6mnFFQYmIC0r4z8rfUgAH4Xag 16, 25 -- In-Reply-To: {200812060033.mB60XlOs030190-at-ns.microscopy.com} 16, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
We've had a similar problem with an imminent TEM installation and so we've explored what is possible to ameliorate for bad acoustics. Above 100Hz things like heavy matting and panels work reasonably well, with better attenuation in the high frequency range (about 5-10dB per one-third octave) for 200-400Hz.
Below 100Hz, things get tricky. You need solid walls with some high mass behind them for the lowest frequencies. If you have space, build a solid brick wall a 'room within a room', but this is expensive. It may be better to get an acoustic survey to see if you have a particular frequency band that is problematic and find the source and dampen that.
David Muller's Ultramicroscopy paper "Room design for high performance microscopes" (correct me if I am wrong), has been useful and is worth consulting.
Yours, Jon
} On Dec 6 2008, Zia.Rahman-1-at-nasa.gov wrote: } } We are starting a new FIB Lab and having some acoustic issues. I would } like to get recommendations on what kind of acoustic material/product is } the best for a FIB or a TEM Lab? Would someone like to share personal } experiences, good or bad?? And/or recommend particular product/s and } vendors?? } } Thanks. } } Zia ur Rahman } FIB Laboratory Manager, } NASA Johnson Space Center, } Houston, TX } Email: Zia.rahman-1-at-nasa.gov
==============================Original Headers============================== 6, 28 -- From jsb43-at-hermes.cam.ac.uk Sat Dec 6 05:20:48 2008 6, 28 -- Received: from ppsw-6.csi.cam.ac.uk (ppsw-6.csi.cam.ac.uk [131.111.8.136]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB6BKlPa002894 6, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 05:20:48 -0600 6, 28 -- X-Cam-AntiVirus: no malware found 6, 28 -- X-Cam-SpamDetails: not scanned 6, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 6, 28 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:55548) 6, 28 -- by ppsw-6.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25) 6, 28 -- with esmtpa (EXTERNAL:jsb43) id 1L8vDL-0002uc-K1 (Exim 4.70) 6, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Sat, 06 Dec 2008 11:20:47 +0000 6, 28 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 6, 28 -- with local (PRAYER:jsb43) id 1L8vDL-0001A9-67 (Exim 4.67) 6, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Sat, 06 Dec 2008 11:20:47 +0000 6, 28 -- Received: from [86.134.143.58] by webmail.hermes.cam.ac.uk 6, 28 -- with HTTP (Prayer-1.3.1); 06 Dec 2008 11:20:47 +0000 6, 28 -- Date: 06 Dec 2008 11:20:47 +0000 6, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 6, 28 -- To: Zia.Rahman-1-at-nasa.gov 6, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 6, 28 -- Subject: Re: [Microscopy] FIB/TEM Acoustic Damping in Labs. 6, 28 -- Message-ID: {Prayer.1.3.1.0812061120470.26766-at-hermes-1.csi.cam.ac.uk} 6, 28 -- In-Reply-To: {200812060038.mB60cIuF003504-at-ns.microscopy.com} 6, 28 -- References: {200812060038.mB60cIuF003504-at-ns.microscopy.com} 6, 28 -- X-Mailer: Prayer v1.3.1 6, 28 -- Mime-Version: 1.0 6, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 6, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
vray-at-partbeamsystech.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Zia, } } You a lucky if this only an acoustic problem and is not accompanied by floor } vibrations. } } As to noise insulation - I can't be sure if my DIY solution will be suitable } for your situation, but little more then two years ago I was setting a } SEM/FIB lab in industrial location and had to deal with excessive noise - } woodworking shop on other side of the building, but separated by only a } drywall from me. Specialized acoustic foams were out of question for cost } reasons, so out of necessity I devised noise-dampening panels, made from two } layers of heat-insulating foam from Home Depot and.... sandwiched between } the layers of foam 12" x 12" egg trays made from recycled pulp (paper), } which were obtained free of charge from one of local businesses. "Liquid } Nails" construction glue was used to hold everything together, and the } result was... well, deafening :) I did not conduct any acoustic } measurements, but when the offending wall was covered by these home-made } panels (same "Liquid Nails" glue for mounting to drywall) previously loud } disk saws running across the wall could be barely heard, and that is only } when all my switched off; fans of three PCs were about as loud as the } outside noise - good enough for my purposes. } } If anyone wants more details I can look up which foam was used and send } detailed explanation on how panels were sandwiched together and finished. I } believe that for compliance with building codes paper egg cartons must be } sprayed with fire retardant before they allowed in construction, though I } did not worry about it at the time. } } Cheers, } Valery Ray } } ============================ } www.partbeamsystech.com } PBS&T, MEO Engineering Co, Inc. } 290 Broadway St., Suite 298 } Methuen, MA 01844 } Phone: (978) 296-5063 } } -----Original Message----- } X-from: Zia.Rahman-1-at-nasa.gov [mailto:Zia.Rahman-1-at-nasa.gov] } Sent: Friday, December 05, 2008 7:34 PM } To: vray-at-partbeamsystech.com } Subject: [Microscopy] FIB/TEM Acoustic Damping in Labs. } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We are starting a new FIB Lab and having some acoustic issues. I would } like to get recommendations on what kind of acoustic material/product is } the best for a FIB or a TEM Lab? Would someone like to share personal } experiences, good or bad?? And/or recommend particular product/s and } vendors?? } } Thanks. } } Zia ur Rahman } FIB Laboratory Manager, } NASA Johnson Space Center, } Houston, TX } Email: Zia.rahman-1-at-nasa.gov } } } ==============================Original Headers============================== } 4, 29 -- From Zia.Rahman-1-at-nasa.gov Fri Dec 5 18:32:02 2008 } 4, 29 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov } [198.117.0.123]) } 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mB60W2Ls028620 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:02 } -0600 } 4, 29 -- Received: from ndjsppt03.ndc.nasa.gov (ndjsppt03.ndc.nasa.gov } [198.117.1.102]) } 4, 29 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id } 797182D801C } 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 } -0600 (CST) } 4, 29 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov } [129.166.32.111]) } 4, 29 -- by ndjsppt03.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id } mB60W1n9016251 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 } -0600 } 4, 29 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by } ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); } 4, 29 -- Fri, 5 Dec 2008 18:32:01 -0600 } 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 4, 29 -- Content-class: urn:content-classes:message } 4, 29 -- MIME-Version: 1.0 } 4, 29 -- Content-Type: text/plain; } 4, 29 -- charset="us-ascii" } 4, 29 -- Subject: FIB/TEM Acoustic Damping in Labs. } 4, 29 -- Date: Fri, 5 Dec 2008 18:32:00 -0600 } 4, 29 -- Message-ID: } {B385F8289FD0B144A75B3A8C3E434E2F2651B1-at-NDJSEVS25A.ndc.nasa.gov} } 4, 29 -- X-MS-Has-Attach: } 4, 29 -- X-MS-TNEF-Correlator: } 4, 29 -- Thread-Topic: FIB/TEM Acoustic Damping in Labs. } 4, 29 -- Thread-Index: AclXOg2EjoVzYyhPQrag5AMgoVOGug== } 4, 29 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} } 4, 29 -- To: {Microscopy-at-microscopy.com} } 4, 29 -- X-OriginalArrivalTime: 06 Dec 2008 00:32:01.0303 (UTC) } FILETIME=[0E195270:01C9573A] } 4, 29 -- Content-Transfer-Encoding: 8bit } 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mB60W2Ls028620 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 16, 25 -- From vray-at-partbeamsystech.com Sat Dec 6 00:17:38 2008 } 16, 25 -- Received: from smtp100.biz.mail.re2.yahoo.com (smtp100.biz.mail.re2.yahoo.com [206.190.52.46]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB66HbAZ015447 } 16, 25 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 00:17:38 -0600 } 16, 25 -- Message-Id: {200812060617.mB66HbAZ015447-at-ns.microscopy.com} } 16, 25 -- Received: (qmail 40967 invoked from network); 6 Dec 2008 06:17:37 -0000 } 16, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) } 16, 25 -- by smtp100.biz.mail.re2.yahoo.com with SMTP; 6 Dec 2008 06:17:37 -0000 } 16, 25 -- X-YMail-OSG: g5J6aRgVM1lF8lJ1.Zsv.MxUmfZBk2i79eug8g2G.wWT4bBOFWrnh1ytcOMTdqVd.xnxKftupnUg29l66hlkq4mVFabVR3fsqZE.LpEiOrB6EcBsx6gZALjTkQtL_NtVvB8DI7zyM8xQEa3LFBEg2q9Axzg8_RVKrRBmyUo1kx.4T4XfjFpHPjvqvvjYH_u9j7_I5qhXX5VQYfSBicrSBuS1AQLeaWnEmkzI5cnLubdrgUa9ylh6PDhYvkIa } 16, 25 -- X-Yahoo-Newman-Property: ymail-3 } 16, 25 -- Reply-To: {vray-at-partbeamsystech.com} } 16, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} } 16, 25 -- To: {Zia.Rahman-1-at-nasa.gov} } 16, 25 -- Cc: {microscopy-at-microscopy.com} } 16, 25 -- Subject: RE: [Microscopy] FIB/TEM Acoustic Damping in Labs. } 16, 25 -- Date: Sat, 6 Dec 2008 01:20:15 -0500 } 16, 25 -- Organization: PBST / MEO Engineering } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- Content-Type: text/plain; } 16, 25 -- charset="us-ascii" } 16, 25 -- Content-Transfer-Encoding: 7bit } 16, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 } 16, 25 -- Thread-Index: AclXOk7jU8z6mnFFQYmIC0r4z8rfUgAH4Xag } 16, 25 -- In-Reply-To: {200812060033.mB60XlOs030190-at-ns.microscopy.com} } 16, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 21 -- From dac-at-research.umass.edu Sat Dec 6 07:28:43 2008 5, 21 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB6DShhL018922 5, 21 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 07:28:43 -0600 5, 21 -- Received: from [192.168.1.100] (static.unknown.charter.com [96.39.6.64] (may be forged)) 5, 21 -- (authenticated bits=0) 5, 21 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id mB6DSgYq017594 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 21 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 08:28:42 -0500 5, 21 -- Message-ID: {493A7E25.2030001-at-research.umass.edu} 5, 21 -- Date: Sat, 06 Dec 2008 08:29:09 -0500 5, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.18) Gecko/20081031 SeaMonkey/1.1.13 5, 21 -- MIME-Version: 1.0 5, 21 -- To: Microscopy List {microscopy-at-microscopy.com} 5, 21 -- Subject: Re: [Microscopy] RE: FIB/TEM Acoustic Damping in Labs - The Station 5, 21 -- Fire? 5, 21 -- References: {200812060622.mB66MkQU023591-at-ns.microscopy.com} 5, 21 -- In-Reply-To: {200812060622.mB66MkQU023591-at-ns.microscopy.com} 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I seek comments from the subscribers on the merits of JEOL's 5000 series (5800 and 5900) vs 6000 series (6300 and 6400) SEM's. I am particularly interested in the variable pressure instruments. I would also like to know if the 6300 xv is a variable pressure model? There is limited information on the web.
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 7, 31 -- From mmcheath-at-syr.edu Sat Dec 6 09:41:10 2008 7, 31 -- Received: from smtp1.syr.edu (smtp1.syr.edu [128.230.18.82]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB6FfAnH003052 7, 31 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 09:41:10 -0600 7, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 7, 31 -- by smtp1.syr.edu (8.14.3/8.14.3) with ESMTP id mB6Ff9pQ025465 7, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 7, 31 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 10:41:10 -0500 7, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 7, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 7, 31 -- 8.1.291.1; Sat, 6 Dec 2008 10:41:09 -0500 7, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 7, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Sat, 6 Dec 2008 10:41:09 -0500 7, 31 -- Received: from 67.241.11.193 ([67.241.11.193]) by SUEXCL-02.ad.syr.edu 7, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 7, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Sat, 6 Dec 7, 31 -- 2008 15:41:09 +0000 7, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 7, 31 -- Date: Sat, 6 Dec 2008 10:41:08 -0500 7, 31 -- Subject: JEOL SEM's: comments on 5000 series vs 6000 series 7, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 7, 31 -- To: {microscopy-at-microscopy.com} 7, 31 -- Message-ID: {C5600744.2A96C%mmcheath-at-syr.edu} 7, 31 -- Thread-Topic: JEOL SEM's: comments on 5000 series vs 6000 series 7, 31 -- Thread-Index: AclXuQ52dDDGm9yhXEKcdZeqk4c+4Q== 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; charset="US-ASCII" 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- X-OriginalArrivalTime: 06 Dec 2008 15:41:09.0684 (UTC) FILETIME=[0F77B740:01C957B9] 7, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-12-05_10:2008-12-05,2008-12-05,2008-12-05 signatures=0 7, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
Dale, keep rock bands with pyrotechnics out of the lab, would ya? :))))))))
Flammability is a valid concern, "Poly Shield" foam (the softest and most dampening I found at about $10/sheet) allowed to make lightweight and low-cost panels (came to about $25 in total material costs for 4' x 8' panel), but it is very flammable and can't be left exposed; building code requires fire and thermal barrier. Check with local building inspector, but I believe that 5/8" drywall would be needed to get back into full compliance (adds about $7 material costs for 4' x 8'), then finish of your choice.
In my case the offending wall did not have electrical wiring and nothing but shelves with tools and parts adjacent to it, so I forgone the drywall and later went with acrylic melamine tile boards (smooth and easy to clean, never need painting, just keep them dry) right above the foam, which is a calculated risk. Drywall installation in the room where SEM column was worked on did not seem like a good idea at the time, but in institutional building liability concerns will take priority.
Cheers, Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: dac-at-research.umass.edu [mailto:dac-at-research.umass.edu] Sent: Saturday, December 06, 2008 8:30 AM To: vray-at-partbeamsystech.com
Foam insulation from Home Depot? Ooooh... that sounds like a potential liability...
vray-at-partbeamsystech.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Zia, } } You a lucky if this only an acoustic problem and is not accompanied by floor } vibrations. } } As to noise insulation - I can't be sure if my DIY solution will be suitable } for your situation, but little more then two years ago I was setting a } SEM/FIB lab in industrial location and had to deal with excessive noise - } woodworking shop on other side of the building, but separated by only a } drywall from me. Specialized acoustic foams were out of question for cost } reasons, so out of necessity I devised noise-dampening panels, made from two } layers of heat-insulating foam from Home Depot and.... sandwiched between } the layers of foam 12" x 12" egg trays made from recycled pulp (paper), } which were obtained free of charge from one of local businesses. "Liquid } Nails" construction glue was used to hold everything together, and the } result was... well, deafening :) I did not conduct any acoustic } measurements, but when the offending wall was covered by these home-made } panels (same "Liquid Nails" glue for mounting to drywall) previously loud } disk saws running across the wall could be barely heard, and that is only } when all my switched off; fans of three PCs were about as loud as the } outside noise - good enough for my purposes. } } If anyone wants more details I can look up which foam was used and send } detailed explanation on how panels were sandwiched together and finished. I } believe that for compliance with building codes paper egg cartons must be } sprayed with fire retardant before they allowed in construction, though I } did not worry about it at the time. } } Cheers, } Valery Ray } } ============================ } www.partbeamsystech.com } PBS&T, MEO Engineering Co, Inc. } 290 Broadway St., Suite 298 } Methuen, MA 01844 } Phone: (978) 296-5063 } } -----Original Message----- } X-from: Zia.Rahman-1-at-nasa.gov [mailto:Zia.Rahman-1-at-nasa.gov] } Sent: Friday, December 05, 2008 7:34 PM } To: vray-at-partbeamsystech.com } Subject: [Microscopy] FIB/TEM Acoustic Damping in Labs. } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We are starting a new FIB Lab and having some acoustic issues. I would } like to get recommendations on what kind of acoustic material/product is } the best for a FIB or a TEM Lab? Would someone like to share personal } experiences, good or bad?? And/or recommend particular product/s and } vendors?? } } Thanks. } } Zia ur Rahman } FIB Laboratory Manager, } NASA Johnson Space Center, } Houston, TX } Email: Zia.rahman-1-at-nasa.gov } } } ==============================Original Headers============================== } 4, 29 -- From Zia.Rahman-1-at-nasa.gov Fri Dec 5 18:32:02 2008 } 4, 29 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov } [198.117.0.123]) } 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mB60W2Ls028620 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:02 } -0600 } 4, 29 -- Received: from ndjsppt03.ndc.nasa.gov (ndjsppt03.ndc.nasa.gov } [198.117.1.102]) } 4, 29 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id } 797182D801C } 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 } -0600 (CST) } 4, 29 -- Received: from ndjsxgw03.ndc.nasa.gov (ndjsxgw03.ndc.nasa.gov } [129.166.32.111]) } 4, 29 -- by ndjsppt03.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id } mB60W1n9016251 } 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Dec 2008 18:32:01 } -0600 } 4, 29 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by } ndjsxgw03.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); } 4, 29 -- Fri, 5 Dec 2008 18:32:01 -0600 } 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 4, 29 -- Content-class: urn:content-classes:message } 4, 29 -- MIME-Version: 1.0 } 4, 29 -- Content-Type: text/plain; } 4, 29 -- charset="us-ascii" } 4, 29 -- Subject: FIB/TEM Acoustic Damping in Labs. } 4, 29 -- Date: Fri, 5 Dec 2008 18:32:00 -0600 } 4, 29 -- Message-ID: } {B385F8289FD0B144A75B3A8C3E434E2F2651B1-at-NDJSEVS25A.ndc.nasa.gov} } 4, 29 -- X-MS-Has-Attach: } 4, 29 -- X-MS-TNEF-Correlator: } 4, 29 -- Thread-Topic: FIB/TEM Acoustic Damping in Labs. } 4, 29 -- Thread-Index: AclXOg2EjoVzYyhPQrag5AMgoVOGug== } 4, 29 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} } 4, 29 -- To: {Microscopy-at-microscopy.com} } 4, 29 -- X-OriginalArrivalTime: 06 Dec 2008 00:32:01.0303 (UTC) } FILETIME=[0E195270:01C9573A] } 4, 29 -- Content-Transfer-Encoding: 8bit } 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mB60W2Ls028620 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 16, 25 -- From vray-at-partbeamsystech.com Sat Dec 6 00:17:38 2008 } 16, 25 -- Received: from smtp100.biz.mail.re2.yahoo.com (smtp100.biz.mail.re2.yahoo.com [206.190.52.46]) } 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB66HbAZ015447 } 16, 25 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 00:17:38 -0600 } 16, 25 -- Message-Id: {200812060617.mB66HbAZ015447-at-ns.microscopy.com} } 16, 25 -- Received: (qmail 40967 invoked from network); 6 Dec 2008 06:17:37 -0000 } 16, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) } 16, 25 -- by smtp100.biz.mail.re2.yahoo.com with SMTP; 6 Dec 2008 06:17:37 -0000 } 16, 25 -- X-YMail-OSG: g5J6aRgVM1lF8lJ1.Zsv.MxUmfZBk2i79eug8g2G.wWT4bBOFWrnh1ytcOMTdqVd.xnxKftupnUg 29l66hlkq4mVFabVR3fsqZE.LpEiOrB6EcBsx6gZALjTkQtL_NtVvB8DI7zyM8xQEa3LFBEg2q9A xzg8_RVKrRBmyUo1kx.4T4XfjFpHPjvqvvjYH_u9j7_I5qhXX5VQYfSBicrSBuS1AQLeaWnEmkzI 5cnLubdrgUa9ylh6PDhYvkIa } 16, 25 -- X-Yahoo-Newman-Property: ymail-3 } 16, 25 -- Reply-To: {vray-at-partbeamsystech.com} } 16, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} } 16, 25 -- To: {Zia.Rahman-1-at-nasa.gov} } 16, 25 -- Cc: {microscopy-at-microscopy.com} } 16, 25 -- Subject: RE: [Microscopy] FIB/TEM Acoustic Damping in Labs. } 16, 25 -- Date: Sat, 6 Dec 2008 01:20:15 -0500 } 16, 25 -- Organization: PBST / MEO Engineering } 16, 25 -- MIME-Version: 1.0 } 16, 25 -- Content-Type: text/plain; } 16, 25 -- charset="us-ascii" } 16, 25 -- Content-Transfer-Encoding: 7bit } 16, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 } 16, 25 -- Thread-Index: AclXOk7jU8z6mnFFQYmIC0r4z8rfUgAH4Xag } 16, 25 -- In-Reply-To: {200812060033.mB60XlOs030190-at-ns.microscopy.com} } 16, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 21 -- From dac-at-research.umass.edu Sat Dec 6 07:28:43 2008 5, 21 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB6DShhL018922 5, 21 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 07:28:43 -0600 5, 21 -- Received: from [192.168.1.100] (static.unknown.charter.com [96.39.6.64] (may be forged)) 5, 21 -- (authenticated bits=0) 5, 21 -- by race2.oit.umass.edu (8.14.3/8.14.3) with ESMTP id mB6DSgYq017594 5, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 21 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 08:28:42 -0500 5, 21 -- Message-ID: {493A7E25.2030001-at-research.umass.edu} 5, 21 -- Date: Sat, 06 Dec 2008 08:29:09 -0500 5, 21 -- From: Dale Callaham {dac-at-research.umass.edu} 5, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.18) Gecko/20081031 SeaMonkey/1.1.13 5, 21 -- MIME-Version: 1.0 5, 21 -- To: Microscopy List {microscopy-at-microscopy.com} 5, 21 -- Subject: Re: [Microscopy] RE: FIB/TEM Acoustic Damping in Labs - The Station 5, 21 -- Fire? 5, 21 -- References: {200812060622.mB66MkQU023591-at-ns.microscopy.com} 5, 21 -- In-Reply-To: {200812060622.mB66MkQU023591-at-ns.microscopy.com} 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 17, 25 -- From vray-at-partbeamsystech.com Sat Dec 6 10:58:14 2008 17, 25 -- Received: from smtp110.biz.mail.re2.yahoo.com (smtp110.biz.mail.re2.yahoo.com [206.190.53.9]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB6GwEDi018844 17, 25 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 10:58:14 -0600 17, 25 -- Message-Id: {200812061658.mB6GwEDi018844-at-ns.microscopy.com} 17, 25 -- Received: (qmail 79870 invoked from network); 6 Dec 2008 16:58:13 -0000 17, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) 17, 25 -- by smtp110.biz.mail.re2.yahoo.com with SMTP; 6 Dec 2008 16:58:13 -0000 17, 25 -- X-YMail-OSG: OCDYjAcVM1nNoCsprwMVyWnyZYd_OmjHI1x23yD7ZJBqE2JlIAb7zRl0iqGqsLyXkepkv1Q_N7GTtocNlvVoKuoBi48AfkOxNDTWuukFQPcaLR5YxsNy9sNMoK18bAQEjFbE6fmzFV1AIJPaSEloamGU_ZBkprLUtY2QY2Ub5rQFIpFLjvpJLanRMckChaXCOfFQhm9BnsThYzgIgvEZ0DenpFGAHfZUkismVHirtcTZjj4ncb1A07eDdMNV 17, 25 -- X-Yahoo-Newman-Property: ymail-3 17, 25 -- Reply-To: {vray-at-partbeamsystech.com} 17, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 17, 25 -- To: {dac-at-research.umass.edu} 17, 25 -- Cc: {microscopy-at-microscopy.com} 17, 25 -- Subject: RE: [Microscopy] FIB/TEM Acoustic Damping in Labs - The Station 17, 25 -- Date: Sat, 6 Dec 2008 12:00:55 -0500 17, 25 -- Organization: PBST / MEO Engineering 17, 25 -- MIME-Version: 1.0 17, 25 -- Content-Type: text/plain; 17, 25 -- charset="us-ascii" 17, 25 -- Content-Transfer-Encoding: 7bit 17, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 17, 25 -- Thread-Index: AclXprgAhqMN15ZqQ0KF1j/XfCP1yAAFRNlg 17, 25 -- In-Reply-To: {200812061329.mB6DTpL3020480-at-ns.microscopy.com} 17, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Mike, I can't help you out on the variable pressure, but, in general, I prefer the 6xxx to the 5xxx (I've only worked on 5200, 5300 and 5400) because the vacuum system, stage and column of the 6xxx seem to me to be far superior. I've also found the electronics on the 6xxx to be easier to work on, and there is generally much more flexibility in the operation of the 6xxx. Of course this is heavily reflected in the pricing. What are you looking to do, specifically?
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: mmcheath-at-syr.edu [mailto:mmcheath-at-syr.edu] Sent: Saturday, December 06, 2008 10:44 AM To: kenconverse-at-qualityimages.biz
Hi,
I seek comments from the subscribers on the merits of JEOL's 5000 series (5800 and 5900) vs 6000 series (6300 and 6400) SEM's. I am particularly interested in the variable pressure instruments. I would also like to know if the 6300 xv is a variable pressure model? There is limited information on the web.
TIA Mike
******************************************************************** Michael M. Cheatham 312 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html owner of SIRIS-L: http://listserv.syr.edu/archives/siris-l.html ********************************************************************
==============================Original Headers============================== 7, 31 -- From mmcheath-at-syr.edu Sat Dec 6 09:41:10 2008 7, 31 -- Received: from smtp1.syr.edu (smtp1.syr.edu [128.230.18.82]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB6FfAnH003052 7, 31 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 09:41:10 -0600 7, 31 -- Received: from suex07-hub-01.ad.syr.edu (suex07-hub-01.ad.syr.edu [128.230.108.195]) 7, 31 -- by smtp1.syr.edu (8.14.3/8.14.3) with ESMTP id mB6Ff9pQ025465 7, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 7, 31 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 10:41:10 -0500 7, 31 -- Received: from suexmx-01.ad.syr.edu (128.230.108.65) by 7, 31 -- suex07-hub-01.ad.syr.edu (128.230.108.195) with Microsoft SMTP Server id 7, 31 -- 8.1.291.1; Sat, 6 Dec 2008 10:41:09 -0500 7, 31 -- Received: from SUEXCL-02.ad.syr.edu ([128.230.108.46]) by suexmx-01.ad.syr.edu 7, 31 -- with Microsoft SMTPSVC(6.0.3790.3959); Sat, 6 Dec 2008 10:41:09 -0500 7, 31 -- Received: from 67.241.11.193 ([67.241.11.193]) by SUEXCL-02.ad.syr.edu 7, 31 -- ([128.230.108.53]) via Exchange Front-End Server exchange.syr.edu 7, 31 -- ([128.230.108.194]) with Microsoft Exchange Server HTTP-DAV ; Sat, 6 Dec 7, 31 -- 2008 15:41:09 +0000 7, 31 -- User-Agent: Microsoft-Entourage/12.12.0.080729 7, 31 -- Date: Sat, 6 Dec 2008 10:41:08 -0500 7, 31 -- Subject: JEOL SEM's: comments on 5000 series vs 6000 series 7, 31 -- From: Michael Cheatham {mmcheath-at-syr.edu} 7, 31 -- To: {microscopy-at-microscopy.com} 7, 31 -- Message-ID: {C5600744.2A96C%mmcheath-at-syr.edu} 7, 31 -- Thread-Topic: JEOL SEM's: comments on 5000 series vs 6000 series 7, 31 -- Thread-Index: AclXuQ52dDDGm9yhXEKcdZeqk4c+4Q== 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; charset="US-ASCII" 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- X-OriginalArrivalTime: 06 Dec 2008 15:41:09.0684 (UTC) FILETIME=[0F77B740:01C957B9] 7, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=1.12.7400:2.4.4,1.2.40,4.0.166 definitions=2008-12-05_10:2008-12-05,2008-12-05,2008-12-05 signatures=0 7, 31 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
==============================Original Headers============================== 19, 25 -- From kenconverse-at-qualityimages.biz Sat Dec 6 14:35:53 2008 19, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB6KZr9w005071 19, 25 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 14:35:53 -0600 19, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta02.mail.rr.com 19, 25 -- with ESMTP 19, 25 -- id {20081206203552.BOCA27302.cdptpa-omta02.mail.rr.com-at-Ken} ; 19, 25 -- Sat, 6 Dec 2008 20:35:52 +0000 19, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 19, 25 -- To: {mmcheath-at-syr.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 19, 25 -- Subject: RE: [Microscopy] JEOL SEM's: comments on 5000 series vs 6000 series 19, 25 -- Date: Sat, 6 Dec 2008 15:35:43 -0500 19, 25 -- Message-ID: {0B6381BFB71D49FDB498D2DE581D2F1C-at-Ken} 19, 25 -- MIME-Version: 1.0 19, 25 -- Content-Type: text/plain; 19, 25 -- charset="us-ascii" 19, 25 -- X-Priority: 3 (Normal) 19, 25 -- X-MSMail-Priority: Normal 19, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 19, 25 -- Importance: Normal 19, 25 -- Thread-Index: AclXuXbFpp4UJ0WHTEa+zlpvFcNdXAAJt2Ug 19, 25 -- In-Reply-To: {200812061544.mB6Fi3gA007087-at-ns.microscopy.com} 19, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 19, 25 -- Content-Transfer-Encoding: 8bit 19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB6KZr9w005071 ==============================End of - Headers==============================
I work as researcher scientist in the Microelectronics Research Center of The University of Texas at Austin.
We recently purchased a Plasma Cleaner (Southbay Technologies, model PC2000) and I am trying to find out what is the best O2/Ar ratio for the plasma composition prior to 200 kV TEM (imaging and EELS analysis) of silicon specimens?
Best regards,
Domingo
Domingo Ferrer, Ph.D. Research Scientist The University of Texas at Austin Microelectronics Research Center, 10100 Burnet Road, MER 1.606J /R9900, Austin, TX 78758-4445 phone number: (office) 512-2325917 / (fax) 512-4718575 / (mobile) 512-7857773 e-mail: domingo-at-mer.utexas.edu
==============================Original Headers============================== 9, 23 -- From dferrerluppi-at-yahoo.com Sat Dec 6 18:54:01 2008 9, 23 -- Received: from web57004.mail.re3.yahoo.com (web57004.mail.re3.yahoo.com [66.196.97.108]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB70s1NE023361 9, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 18:54:01 -0600 9, 23 -- Received: (qmail 82249 invoked by uid 60001); 7 Dec 2008 00:54:00 -0000 9, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 23 -- s=s1024; d=yahoo.com; 9, 23 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 9, 23 -- b=SHBB88/HPkkKMgQkzwUuPPd8mrxpel1tX4YwFWlqiJ6bgvBKago31Jdg98LDTI5aEtBobxBI/3HWCbk1AsQeCmhoH5LkeUquTVDEKAQc65y9T/0lVjKacH7zSdoxIK2WDZW+dbzZWq1jfFaXGTSAU+zeNOasTuwx39HuQF4JLrE=; 9, 23 -- X-YMail-OSG: Ouk.Jr8VM1krLJQLkm6on__9KNUzwb.UZZq.KxProbz44x.6CGGXuuGVp.6OimWcorU9vwctbHmJSx7BmQliSJD.SWirchDOtAHNhoNCycsvHDTi9VQRgmXtYE0.oFLvr_holxn1nmZW4Ahtx5Dy5NLSURyISGpj_Fzu6GzMQdWkPj_Kv8t_aK2SO5E- 9, 23 -- Received: from [129.116.234.216] by web57004.mail.re3.yahoo.com via HTTP; Sat, 06 Dec 2008 16:54:00 PST 9, 23 -- X-Mailer: YahooMailWebService/0.7.260.1 9, 23 -- Date: Sat, 6 Dec 2008 16:54:00 -0800 (PST) 9, 23 -- From: Domingo Ferrer Luppi {dferrerluppi-at-yahoo.com} 9, 23 -- Reply-To: dferrerluppi-at-yahoo.com 9, 23 -- Subject: plasma cleaner O2/Ar ratio 9, 23 -- To: Microscopy-at-microscopy.com 9, 23 -- Cc: dferrer-at-che.utexas.edu 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=utf-8 9, 23 -- Message-ID: {596638.81786.qm-at-web57004.mail.re3.yahoo.com} 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB70s1NE023361 ==============================End of - Headers==============================
I'd suggest that you check out each unit in person and with specific specimens that "require" VP. So far, I find VP to be rather useless above about 10KX. The extra costs of VP (ion pump, bypass valves, special detector, etc.) just don't produce an image worth the cost. Column alignment is also going to be more difficult and less stable probably.
If charging is an issue, check out units that image well at low KV. The older LEO/Zeiss VP SEMs do a poor job of imaging at VP. The Zeiss Supra VP units are better but not a home run. The Zeiss Ultra IIVP is supposed to be much better based on pin point injection of gas rather than making the whole chamber the same Pa. This helps greatly at wide ranges of WD and reduces the complexity of the VP system.
VP is going to add a lot of additional vacuum hoses and interconnections that will probably lead to degraded resolution at high vacuum. All major VP items are removed from my Zeiss Supra 55VP. I miss them not. for charging samples, low KV works great. 1KV is fine. The downside is DOF based on WD. The E-T works over a very wide range of WD but closes in at low KV and long WD. To get a better pix, increase KV and decrease WD...then deal with charging again if it happens.
Depending on your application, perhaps an EVO-type SEM would be a better choice? I have no experience with the JEOLs. It would be interesting to hear your feedback about them versus the Zeiss and others.
gary g.
At 07:42 AM 12/6/2008, you wrote:
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==============================Original Headers============================== 12, 21 -- From gary-at-gaugler.com Sat Dec 6 19:25:16 2008 12, 21 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB71PGwT004772 12, 21 -- for {microscopy-at-microscopy.com} ; Sat, 6 Dec 2008 19:25:16 -0600 12, 21 -- Message-Id: {200812070125.mB71PGwT004772-at-ns.microscopy.com} 12, 21 -- Received: (qmail 25321 invoked from network); 6 Dec 2008 17:24:31 -0800 12, 21 -- Received: by simscan 1.1.0 ppid: 25317, pid: 25319, t: 0.2118s 12, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 12, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 12, 21 -- by smtp1 with SMTP; 6 Dec 2008 17:24:31 -0800 12, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 21 -- Date: Sat, 06 Dec 2008 17:25:14 -0800 12, 21 -- To: mmcheath-at-syr.edu 12, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 12, 21 -- Subject: Re: [Microscopy] JEOL SEM's: comments on 5000 series vs 6000 12, 21 -- series 12, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 12, 21 -- In-Reply-To: {200812061542.mB6FgkWT005492-at-ns.microscopy.com} 12, 21 -- References: {200812061542.mB6FgkWT005492-at-ns.microscopy.com} 12, 21 -- Mime-Version: 1.0 12, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
The chemistry of the plasma and how the potential cocktail of gases used in them cleans a sample varies dramatically between the various commerical units. Every unit that I have used works well using the manufacturers recommended conditions, so you should always start there.
However, you do not HAVE to use both O & Ar in a plasma cleaning system to remove hydrocarbons. In most cases in my lab I start using only pure Ar and only resort to adding O when the contamination is severe. The model of plasma cleaner you are using it is a low/medium power plasma and the cleaning process is slow (which is the mode I prefer). I would recommend starting at 10-15 W, for 10 minutes at about 200 mT of Ar. See how it works on your samples and then adjust the time/power/pressure/composition to optimize for your specific specimens.
I have used a range of conditions for different materials and very importantly you should realize that you will likely need different conditions for different ways your specimen has been prepared. For example for nanoparticles on C films I never use O, as the plasma will attack the support film, however, a short low energy Ar plasma works well to remove any residual organics without completely removing the C film. On the other hand for electropolished samples with lots of organic solvents I frequently mix in Oxygen, or in very severe cases use pure O2.
Remember start with your manufacturers recommendations and then tweek the conditions from there.
Disclaimer: The organization I work for Argonne National Laboratory holds the original patent on plasma cleaning for TEM/SEM instruments, and has licensed this technology to commerical vendors.
Nestor Your Friendly Neighborhood SysOp
At 6:54 PM -0600 12/6/08, dferrerluppi-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 12, 13 -- From zaluzec-at-microscopy.com Sun Dec 7 13:19:02 2008 12, 13 -- Received: from [10.158.144.177] (msdvpn8.msd.anl.gov [130.202.238.72]) 12, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB7JIwoi022962 12, 13 -- for {microscopy-at-microscopy.com} ; Sun, 7 Dec 2008 13:19:00 -0600 12, 13 -- Mime-Version: 1.0 12, 13 -- Message-Id: {p06240802c561ccb8736a-at-[10.158.144.177]} 12, 13 -- In-Reply-To: {200812070054.mB70s1WS023375-at-ns.microscopy.com} 12, 13 -- References: {200812070054.mB70s1WS023375-at-ns.microscopy.com} 12, 13 -- Date: Sun, 7 Dec 2008 13:19:12 -0600 12, 13 -- To: microscopy-at-microscopy.com 12, 13 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 12, 13 -- Subject: Re: [Microscopy] plasma cleaner O2/Ar ratio 12, 13 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We have had a JEOL 5800LV for almost 14 years. It gets a ton of work in the VP mode and works very well. Imaging is limited to the backscattered detector in the VP mode, and as Gary noted, resolution is not great at 10K and greater magnification in VP mode. But this instrument performs very well at high vacuum. I do not think that there is any decrease in performance in high vacuum mode compared to a non-VP instrument. Imaging at 50KX is possible and I have worked at voltages as low as 2 KV. Not bad for a tungsten source.
I have limited experience with the 6000 series instruments. I think that the column and detectors were very similar if not identical to the 5800. Some later instruments had greater Z travel than the 5800. The 5800 had an archaic computer control system, whereas the 6000 series models had a regular PC interface and hardware. (I think the 5900 may have had a PC rather than the proprietary computer.) Our 5800 still works great, but I am concerned about availability of parts as time goes on, especially for the computer electronics. I am hopeful that we can keep our 5800 running for a few more years, but we have had a couple of issues finding components.
We start installation of a new JEOL 6610 model next Monday. This instrument has considerable improvements over previous models in the 6000 series. The user interface is new with more imaging options and better stage navigation. There is a new detector for VP mode that provides imaging similar to secondary electron images. The signal to noise on the BS detector is greatly improved and you can get great backscatter images at very low KV.
Hope this helps.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 6, 24 -- From hanke-at-mee-inc.com Sun Dec 7 13:56:13 2008 6, 24 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB7JuDXt006005 6, 24 -- for {microscopy-at-microscopy.com} ; Sun, 7 Dec 2008 13:56:13 -0600 6, 24 -- Received: (qmail 12244 invoked by uid 508); 7 Dec 2008 13:56:12 -0600 6, 24 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 6, 24 -- (clamdscan: 0.87/8729. spamassassin: 3.0.2. perlscan: 1.24-st-qms. 6, 24 -- Clear:RC:1(216.14.180.82):. 6, 24 -- Processed in 0.061207 secs); 07 Dec 2008 19:56:12 -0000 6, 24 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 6, 24 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.061207 secs Process 12237) 6, 24 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 6, 24 -- by mail.namisolutions.com with SMTP; 7 Dec 2008 13:56:12 -0600 6, 24 -- Message-ID: {493C2A56.1030702-at-mee-inc.com} 6, 24 -- Date: Sun, 07 Dec 2008 13:56:06 -0600 6, 24 -- From: Larry Hanke {hanke-at-mee-inc.com} 6, 24 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 6, 24 -- MIME-Version: 1.0 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- Subject: Re: JEOL SEM's: comments on 5000 series vs 6000 6, 24 -- References: {200812070125.mB71PlhO005804-at-ns.microscopy.com} 6, 24 -- In-Reply-To: {200812070125.mB71PlhO005804-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Larry, I don't know about the newer 6xxx models, but the 6300/6400 are not run by a PC which means I can probably keep them running for a long time. There was a thread about a couple of months ago concerning PC operated SEMs. You may gain flexibility with the PC, but the basic SEM is good to go for 20-40 years, while the PC is, by definition, obsolete before it is installed. Currently, upgrades of PCs (when available) run in the range of $25k. You can't just run down to Best Buy and buy a new computer to replace your 80486 running Win 3.1. It ain't gonna work.
As to the column, the 6xxx series has basically an 840 column and vacuum system which bears no resemblance whatsoever to the 5200, 5300 or 5400 (which are very similar to the T2xx/T3xx series). I can't vouch for the 5800.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: hanke-at-mee-inc.com [mailto:hanke-at-mee-inc.com] Sent: Sunday, December 07, 2008 2:58 PM To: kenconverse-at-qualityimages.biz
We have had a JEOL 5800LV for almost 14 years. It gets a ton of work in the VP mode and works very well. Imaging is limited to the backscattered detector in the VP mode, and as Gary noted, resolution is not great at 10K and greater magnification in VP mode. But this instrument performs very well at high vacuum. I do not think that there is any decrease in performance in high vacuum mode compared to a non-VP instrument. Imaging at 50KX is possible and I have worked at voltages as low as 2 KV. Not bad for a tungsten source.
I have limited experience with the 6000 series instruments. I think that the column and detectors were very similar if not identical to the 5800. Some later instruments had greater Z travel than the 5800. The 5800 had an archaic computer control system, whereas the 6000 series models had a regular PC interface and hardware. (I think the 5900 may have had a PC rather than the proprietary computer.) Our 5800 still works great, but I am concerned about availability of parts as time goes on, especially for the computer electronics. I am hopeful that we can keep our 5800 running for a few more years, but we have had a couple of issues finding components.
We start installation of a new JEOL 6610 model next Monday. This instrument has considerable improvements over previous models in the 6000 series. The user interface is new with more imaging options and better stage navigation. There is a new detector for VP mode that provides imaging similar to secondary electron images. The signal to noise on the BS detector is greatly improved and you can get great backscatter images at very low KV.
Hope this helps.
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 6, 24 -- From hanke-at-mee-inc.com Sun Dec 7 13:56:13 2008 6, 24 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB7JuDXt006005 6, 24 -- for {microscopy-at-microscopy.com} ; Sun, 7 Dec 2008 13:56:13 -0600 6, 24 -- Received: (qmail 12244 invoked by uid 508); 7 Dec 2008 13:56:12 -0600 6, 24 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 6, 24 -- (clamdscan: 0.87/8729. spamassassin: 3.0.2. perlscan: 1.24-st-qms.
6, 24 -- Clear:RC:1(216.14.180.82):. 6, 24 -- Processed in 0.061207 secs); 07 Dec 2008 19:56:12 -0000 6, 24 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 6, 24 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.061207 secs Process 12237) 6, 24 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 6, 24 -- by mail.namisolutions.com with SMTP; 7 Dec 2008 13:56:12 -0600 6, 24 -- Message-ID: {493C2A56.1030702-at-mee-inc.com} 6, 24 -- Date: Sun, 07 Dec 2008 13:56:06 -0600 6, 24 -- From: Larry Hanke {hanke-at-mee-inc.com} 6, 24 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 6, 24 -- MIME-Version: 1.0 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- Subject: Re: JEOL SEM's: comments on 5000 series vs 6000 6, 24 -- References: {200812070125.mB71PlhO005804-at-ns.microscopy.com} 6, 24 -- In-Reply-To: {200812070125.mB71PlhO005804-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 25 -- From kenconverse-at-qualityimages.biz Sun Dec 7 16:09:15 2008 20, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB7M9DwH027068 20, 25 -- for {microscopy-at-microscopy.com} ; Sun, 7 Dec 2008 16:09:14 -0600 20, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com 20, 25 -- with ESMTP 20, 25 -- id {20081207220911.KRRR28564.cdptpa-omta01.mail.rr.com-at-Ken} ; 20, 25 -- Sun, 7 Dec 2008 22:09:11 +0000 20, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 25 -- To: {hanke-at-mee-inc.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 25 -- Subject: RE: [Microscopy] Re: JEOL SEM's: comments on 5000 series vs 6000 20, 25 -- Date: Sun, 7 Dec 2008 17:09:06 -0500 20, 25 -- Message-ID: {C5AC0B27908045D1959285D40AA28DFB-at-Ken} 20, 25 -- MIME-Version: 1.0 20, 25 -- Content-Type: text/plain; 20, 25 -- charset="us-ascii" 20, 25 -- X-Priority: 3 (Normal) 20, 25 -- X-MSMail-Priority: Normal 20, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 20, 25 -- Importance: Normal 20, 25 -- Thread-Index: AclYphu94kLnYlU3TQKTos5ZC5l2/QAELwMg 20, 25 -- In-Reply-To: {200812071958.mB7Jw36t010075-at-ns.microscopy.com} 20, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 20, 25 -- Content-Transfer-Encoding: 8bit 20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB7M9DwH027068 ==============================End of - Headers==============================
Hi, Thankyou to everyone who replied to my request. Lots of good info for me to pass on to my colleagues.
For anyone interested, I've summarised the thread below.
Regards,
John Brealey EM Unit Queen Elizabeth Hospital Adelaide South Australia
Hi,
We're currently investigating new TEMs. So CCD cameras are also an issue.
The TEM we're looking for will be your standard biological TEM used for kidneys and tumours, etc. 60 - 100kV.
Some of the cameras appear to be beyond our specs, ie, they operate above 120kV.
Are there any cameras that are not compatible with particular TEMs? Conversely are there any cameras that perform better with a particular model of TEM?
Any advice or comments on this issue greatly appreciated since it's hard to compare cameras off a brochure without the experience of having used one.
Regards,
John Brealey EM Unit Queen Elizabeth Hospital South Australia
Replies so far...
1. Hi John CCD camera work better at lower kV so you are in even better shape the the high kv people. less beam spreading when electrons hit the detector. I am
partial to Gatan cameras but I grew up with them and I am comfortable with the software--as much as I would like to see it improved.
Roseann Csencsits, PhD Donner TEM Facility Manager Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
2. do yourself a favor
stick with Gatan and stay away from SIS
unless of course you want reverse logic and methodical overkill
SIS software is not fast nor user friendly
If you have a multi user facility, dont waste your time
someone will come in and reconfig the software/camera (very easily done) and then your stuck calling some software engineer in colorado who has to email some
guy in germany just to bail you out
if its just you, thats different
I still would choose Gatan in a heartbeat
Gatan is fast, friendly, awesome clarity, easy to use and even has decent analytical functions
the SIS software is like working with Photoshop
overkill
this is just my opinion
Fred
3. Hi John,
There are cameras that are targeted for the Bio market and operate at less than 200kV. Gatan has a couple of models for gen. bio. See their site for what
they call Erlangshen models and also there is a SC1000 model that is fiber optic.
What TEM do you have?
4. John,
We had two JEOL TEM's that were using film until say 2 years ago. Both were converted to digital several months apart and we have AMT. There is one other
scope in an adjoining department and it also has an AMT. The main users are quite happy with the cameras. I do not know the brands of cameras on other
scopes here.
I have not used another digital TEM camera so I am not able to compare. I have used my camera with both 60 and 80 kV - all biological samples: tissue sections, negative stains for virus, and carbon/platinum replicas.
One question I would have is about the service over there. We have good service but the tech who usually comes is only a few states away. One problem that
we had was some dirt particles landed on the top of the camera. (We can not figure out how that happened but I think it was when we had service to the column and something settled down when the scope's viewing screen was
up.) My mind has just gone blank as to the real name of the thing...the part that senses the electrons. Anyway the tech needed to come in and take several
parts apart to get at the screen to dust it off - very gently of course. We have had a few softwear problems that were fixed in a jiffy.
We also moved the TEM across campus. This was a minor problem in that the scope company would not touch the scope to move it until the camera was removed
from the scope (not theirs) and then after the scope was put together in the new location we had to wait until the camera was installed, then scope started
up ...OK'ed by the scope people then get the camera working again....
I know that there was a second camera company which supposedly had as good a camera and cost less but when we compared the two, the AMT gave a nicer image
(personal opinion of the director who was doing the comparison). From this I'd say not to take the opinion of a salesperson but see for your self.
Great to hear of someone getting a new scope! Pat Connelly
5. John - see the cameras from SIA - good prices for cameras that use EXACTLY the same sensors as all the more expensive cameras ( and several sensors
that no one else has) and offer a large field of view even when bottom mounted. They have prices, full specs and full resolution, uncompressed images on
the web site - http://www.sia-cam.com.. There are a few installed in Australia already.
good luck - Bill Miller
6. John-
I would encourage you not to buy a CCD from Olympus SIS. Their cameras are poorly designed and the software is poorly documented and poorly supported. They
seem to know very little about the requirements for TEM. OSIS kept sending us cameras that were defective in some way or didn't fit on our microscope, but they want to charge us $15K to return the thing. We finally
ended up with a Veleta, which has terrible noise, but they won't offer any software to correct it. The first prism for the Veleta had a serious optical
defect, which they refused to fix, until I contacted the Better Business Bureau. Then they acted like they were doing me a big favor. I wish I had gotten a
Gatan CCD, which I used before and liked much better.
Digital Micrograph is vastly superior to iTEM, or whatever they call it. iTEM doesn't support 32-bit images, floating-point, or even negative pixel values, which are necessary to correct the noise. But OSIS couldn't seem to care
less, even though they advertize the camera as having "exceptionally low noise".
-Phil ------------------------------------------ Phil Ahrenkiel, Assistant Professor Nanoscience and Nanoengineering Ph.D. Program South Dakota School of Mines and Technology 501 E. Saint Joseph St. Rapid City, South Dakota 57701 Office: EP 221 Phone: 605-394-5238, Fax: 605-394-2365 Email: Phil.Ahrenkiel-at-sdsmt.edu
7. Hi John,
We have had very good experience with our TVIPS slow scan cameras. We have one 2kX2k Camera on a Tecnai 12 running most of the time at 100 kV and another one
on an FEI Morgagni running at 80-100 kV. The cameras have very good resolution and have not had a problem since we have them (6 and 4 years). We collaborate
with the imaging center here at the University, that also takes em images of medical samples and, once their microscope had problems and they used ours with
the camera, they were very happy with the results. TVIPS produces different scintillators for their cameras and should be able to match it to the voltage you
like to use most. May be this info helps.
Michael Radermacher
University of Vermont Burlington, VT, USA
8. Hi John,
Most of the cameras can be manufactured to work for different voltages. I have just gone through the process of getting a camera for a JEOL 2010. We ended
up with a side mount camera as we have an EELS system underneath. With these, one thing you need to be aware of is what portion of the CCD is exposed to the
beam. In our case, a 1.4 megapixel camera becomes a 1.2 megapixel camera. This is to do with the geometry of the column. For the side mount cameras you
have two options, a direct to CCD image using one 35 mm port or a system which reflects the beam through 90 degrees onto a CCD on the opposite side of the
column. For these you need two 35 mm ports available.
Good luck.
Colin Veitch CSIRO CMSE Geelong Laboratory.
9. For me, it appears best to have a TEM of the mid-range, i.e. max 120 keV, with a side entry CCD with 2k x 2k pixel. Recently, I had the opportunity
to use a Tecnai Spirit / Sphaera G2 BioTWIN. Great for fast high contrast high-through put standard imaging. Sections without section UAc/Lead staining are easily visible, wonderful. Would be my first choice to look at. Add the SIS Veleta side entry. Really large viewing field, excellent images. Nice: High-mag imaging mode starts already a mag = 400 x (not only at 3000 x, as with most older TEMs). Yes, works great and of great value for every day work. If you can afford, add a second CCD slow scan at the bottom (later?). Expensive and sounds crazy, but great for details.
I can imaging that a 120 keV Zeiss or JEOL might be similarly versatile. I have to admit, I like the FEI more ... best regards reinhard
==============================Original Headers============================== 85, 35 -- From john.brealey-at-imvs.sa.gov.au Sun Dec 7 17:13:34 2008 85, 35 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.26.121.14]) 85, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB7NDVqg009355 85, 35 -- for {Microscopy-at-microscopy.com} ; Sun, 7 Dec 2008 17:13:32 -0600 85, 35 -- X-IronPort-AV: E=Sophos;i="4.33,732,1220193000"; 85, 35 -- d="scan'208";a="6259219" 85, 35 -- Received: from unknown (HELO EMSGM302.sagemsmrd01.sa.gov.au) ([10.9.18.85]) 85, 35 -- by mailgate9.sa.gov.au with ESMTP; 08 Dec 2008 09:43:30 +1030 85, 35 -- Received: from ablett.imvs.sa.gov.au (10.20.98.41) by 85, 35 -- EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 85, 35 -- 8.1.263.0; Mon, 8 Dec 2008 09:41:45 +1030 85, 35 -- Received: from localhost (mesh.imvs.sa.gov.au [10.20.98.37]) by 85, 35 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id A4D4A34DEE for 85, 35 -- {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:42:29 +1030 (CST) 85, 35 -- X-Virus-Scanned: by amavisd-new at imvs.sa.gov.au 85, 35 -- Received: from ablett.imvs.sa.gov.au ([10.20.98.41]) by localhost 85, 35 -- (.imvs.sa.gov.au [10.20.98.37]) (amavisd-new, port 10024) with LMTP id 85, 35 -- dsenzncTmxwV for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:41:57 +1030 85, 35 -- (CST) 85, 35 -- Received: from 41347i (iqepc125.imvs.sa.gov.au [10.20.138.125]) by 85, 35 -- ablett.imvs.sa.gov.au (Postfix) with ESMTP id 3215B34E01 for 85, 35 -- {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:41:57 +1030 (CST) 85, 35 -- Reply-To: {john.brealey-at-imvs.sa.gov.au} 85, 35 -- From: John BREALEY {john.brealey-at-imvs.sa.gov.au} 85, 35 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 85, 35 -- Subject: CCD Cameras - Summary of Replies 85, 35 -- Date: Mon, 8 Dec 2008 09:42:02 +1030 85, 35 -- Organization: IMVS 85, 35 -- Message-ID: {000001c958c1$365bd450$7d8a140a-at-41347i} 85, 35 -- MIME-Version: 1.0 85, 35 -- Content-Type: text/plain; charset="US-ASCII" 85, 35 -- Content-Transfer-Encoding: 7bit 85, 35 -- X-Mailer: Microsoft Office Outlook 11 85, 35 -- Thread-Index: AclYwTYz4t98XLtXRwqE9GJEZbtQGw== 85, 35 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I would like to explore whether utilizing energy filtering or EDX mapping would be helpful to definitively identify locations of iron oxide nanoparticles ( also Cd or Se in quantum dot nanoprobes) in cells. These particles are often of similar size to cell components (such as ribosomes) and often density alone is not sufficient to identify them.
My understanding is that energy filtering gives best results with lighter elements while EDX can be superior with heavy elements. These are sort of in between so I do not know which technique would be preferable. We ultimately would like to do tomography to determine distribution of the nanoparticles in relation to cell organelles/components.
I would appreciate hearing from those with personal experience in dealing with like particles or referrals to pertinent articles.
Thanks, Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 6, 29 -- From dsherman-at-purdue.edu Mon Dec 8 08:14:04 2008 6, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8EE4Qe000340 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 08:14:04 -0600 6, 29 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 6, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id mB8EE3vc031862 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:14:04 -0500 6, 29 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 6, 29 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id mB8EE3Bm032288 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:14:03 -0500 6, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Mon, 8 Dec 2008 09:14:03 -0500 6, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 6, 29 -- Mon, 8 Dec 2008 14:14:02 +0000 6, 29 -- User-Agent: Microsoft-Entourage/12.14.0.081024 6, 29 -- Date: Mon, 08 Dec 2008 09:14:02 -0500 6, 29 -- Subject: TEM-Localizing FE-NP using EF or EDX? 6, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 29 -- Message-ID: {C56295DA.36FDB%dsherman-at-exchange.purdue.edu} 6, 29 -- Thread-Topic: TEM-Localizing FE-NP using EF or EDX? 6, 29 -- Thread-Index: AclZPzhZ78eIkKiyQCKgzk401KyKQQ== 6, 29 -- Mime-version: 1.0 6, 29 -- Content-type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-OriginalArrivalTime: 08 Dec 2008 14:14:03.0681 (UTC) FILETIME=[395A7110:01C9593F] 6, 29 -- X-PMX-Version: 5.4.0.320885 6, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
In general Fe and Cd should work due to high intensity white lines and characteristic edges located at reasonable energy losses, so 3 window method will work. In terms of Se, the characteristic edges are located at high loss energies and will have low intensity, perhaps spectral image will work. For energy filtered maps it might be challenging to map Se, however you might want to explore Plasmon loses and low-loss energy spectrum. Often, they will contain useful contrast that will differentiate low-Z elements from the metals.
Disclaimer, my lab provides EELS mapping and EDS mapping services and have long experience with semiconductor and other inorganic materials.
Warm Regards,
Jerzy
*************************** Jerzy Gazda Ph.D. Section Manager - TEM, FIB, SEM Cerium Laboratories LLC 5204 E. Ben White Blvd. MS-512 Austin, TX 78741
(512) 934-5185 vm (512) 622-6600 pgr
www.ceriumlabs.com -----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Monday, December 08, 2008 8:23 AM To: Gazda, Jerzy
I would like to explore whether utilizing energy filtering or EDX mapping would be helpful to definitively identify locations of iron oxide nanoparticles ( also Cd or Se in quantum dot nanoprobes) in cells. These particles are often of similar size to cell components (such as ribosomes) and often density alone is not sufficient to identify them.
My understanding is that energy filtering gives best results with lighter elements while EDX can be superior with heavy elements. These are sort of in between so I do not know which technique would be preferable. We ultimately would like to do tomography to determine distribution of the nanoparticles in relation to cell organelles/components.
I would appreciate hearing from those with personal experience in dealing with like particles or referrals to pertinent articles.
Thanks, Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 6, 29 -- From dsherman-at-purdue.edu Mon Dec 8 08:14:04 2008 6, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8EE4Qe000340 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 08:14:04 -0600 6, 29 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 6, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id mB8EE3vc031862 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:14:04 -0500 6, 29 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 6, 29 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id mB8EE3Bm032288 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:14:03 -0500 6, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Mon, 8 Dec 2008 09:14:03 -0500 6, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 6, 29 -- Mon, 8 Dec 2008 14:14:02 +0000 6, 29 -- User-Agent: Microsoft-Entourage/12.14.0.081024 6, 29 -- Date: Mon, 08 Dec 2008 09:14:02 -0500 6, 29 -- Subject: TEM-Localizing FE-NP using EF or EDX? 6, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 29 -- Message-ID: {C56295DA.36FDB%dsherman-at-exchange.purdue.edu} 6, 29 -- Thread-Topic: TEM-Localizing FE-NP using EF or EDX? 6, 29 -- Thread-Index: AclZPzhZ78eIkKiyQCKgzk401KyKQQ== 6, 29 -- Mime-version: 1.0 6, 29 -- Content-type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-OriginalArrivalTime: 08 Dec 2008 14:14:03.0681 (UTC) FILETIME=[395A7110:01C9593F] 6, 29 -- X-PMX-Version: 5.4.0.320885 6, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
==============================Original Headers============================== 17, 29 -- From Jerzy.Gazda-at-spansion.com Mon Dec 8 09:10:48 2008 17, 29 -- Received: from usausmgw01.spansion.com (usausmgw01.spansion.com [12.110.209.161]) 17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8FAl6n022807 17, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:10:47 -0600 17, 29 -- X-IronPort-AV: E=McAfee;i="5300,2777,5457"; a="2038048" 17, 29 -- Received: from usausexbh1.spansion.com ([10.248.26.58]) 17, 29 -- by usausmgw01.spansion.com with ESMTP; 08 Dec 2008 07:10:47 -0800 17, 29 -- Received: from USAUSEXMBPF1.spansion.com ([10.248.26.54]) by USAUSEXBH1.spansion.com with Microsoft SMTPSVC(6.0.3790.3959); 17, 29 -- Mon, 8 Dec 2008 09:10:47 -0600 17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 29 -- Content-class: urn:content-classes:message 17, 29 -- MIME-Version: 1.0 17, 29 -- Content-Type: text/plain; 17, 29 -- charset="us-ascii" 17, 29 -- Subject: RE: [Microscopy] TEM-Localizing FE-NP using EF or EDX? 17, 29 -- Date: Mon, 8 Dec 2008 09:10:46 -0600 17, 29 -- Message-ID: {B8013C1F41F45F4886A4F71F775DF1582ED93A-at-USAUSEXMBPF1.spansion.com} 17, 29 -- In-Reply-To: {200812081423.mB8ENO2o014033-at-ns.microscopy.com} 17, 29 -- X-MS-Has-Attach: 17, 29 -- X-MS-TNEF-Correlator: 17, 29 -- Thread-Topic: [Microscopy] TEM-Localizing FE-NP using EF or EDX? 17, 29 -- Thread-Index: AclZQIl/cBOtovnVTZyAHzhzLs1+GwABWmVg 17, 29 -- References: {200812081423.mB8ENO2o014033-at-ns.microscopy.com} 17, 29 -- From: "Gazda, Jerzy" {jerzy.gazda-at-ceriumlabs.com} 17, 29 -- To: {dsherman-at-purdue.edu} 17, 29 -- Cc: {microscopy-at-microscopy.com} 17, 29 -- X-OriginalArrivalTime: 08 Dec 2008 15:10:47.0132 (UTC) FILETIME=[25F7D9C0:01C95947] 17, 29 -- Content-Transfer-Encoding: 8bit 17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB8FAl6n022807 ==============================End of - Headers==============================
Lecturers: Tony Wilson, Rolf Borlinghaus, Mario Faretta, Maddalena Collini, Fred Wouters, Gertrude Bunt, Ranieri Bizzarri, Gimmi Ratto, Cesare Usai, Alberto Diaspro, Paolo Bianchini.
Info: email to alberto.diaspro-at-iit.it subject: Genoa School
Please, stay connected with http://www.ebsa2009.org ther will be a plenty of microscopy ... and gnocchi with pesto
All the best and anticipated best wishes for the approaching holidays and more universally for the forthcoming new year...
Alby ----------------------------------------------------- Resistere, Resistere, Resistere! Hold out, Hold out, Hold out! ----------------------------------------------------- Alberto Diaspro Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy fax +39-010314218 - tel +39 0103536426/309; email: diaspro-at-fisica.unige.it - URL: www.lambs.it;
Lecturers: Tony Wilson, Rolf Borlinghaus, Mario Faretta, Maddalena Collini, Fred Wouters, Gertrude Bunt, Ranieri Bizzarri, Gimmi Ratto, Cesare Usai, Alberto Diaspro, Paolo Bianchini.
Info: email to alberto.diaspro-at-iit.it subject: Genoa School
Please, stay connected with http://www.ebsa2009.org ther will be a plenty of microscopy ... and gnocchi with pesto
All the best and anticipated best wishes for the approaching holidays and more universally for the forthcoming new year...
Alby ----------------------------------------------------- Resistere, Resistere, Resistere! Hold out, Hold out, Hold out! ----------------------------------------------------- Alberto Diaspro Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy fax +39-010314218 - tel +39 0103536426/309; email: diaspro-at-fisica.unige.it - URL: www.lambs.it;
It might be important to keep track of JEOL's non-chronological model numbering in this discussion.
As Ken mentioned below, the 6400 is not run by a PC (which is correct), however, the "6xxx models" having an 840 column and vacuum system is a bit inaccurate. The 6490LV I have is not an 840 (or a 6400 for that matter). It is more similar to the 5900. There are significant chronological discontinuities in the JEOL numbering for their tungsten filament SEM models (i.e. the 5900 is a newer model than the 6400). I'm no expert on which model is "newer", but I do know it does get a bit confusing trying to keep track of all the changes.
Hopefully I haven't done anything to add confusion here.
But, to add to the thread, I have a 6490LV and agree with previous comments on it. The PC interface is good, but does have some room for improvement. I have the optional LVSE ("Low Vacuum Secondary Electron") detector and have only had a few opportunities to use it, and with some work it does produce good images. I haven't run any of the earlier JEOL VP instruments so I can't speak about them.
Good Luck! Bryan Bandli
kenconverse-at-qualityimages.biz wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Larry, } I don't know about the newer 6xxx models, but the 6300/6400 are not run by a } PC which means I can probably keep them running for a long time. There was } a thread about a couple of months ago concerning PC operated SEMs. You may } gain flexibility with the PC, but the basic SEM is good to go for 20-40 } years, while the PC is, by definition, obsolete before it is installed. } Currently, upgrades of PCs (when available) run in the range of $25k. You } can't just run down to Best Buy and buy a new computer to replace your 80486 } running Win 3.1. It ain't gonna work. } } As to the column, the 6xxx series has basically an 840 column and vacuum } system which bears no resemblance whatsoever to the 5200, 5300 or 5400 } (which are very similar to the T2xx/T3xx series). I can't vouch for the } 5800. } }
} Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: hanke-at-mee-inc.com [mailto:hanke-at-mee-inc.com] } Sent: Sunday, December 07, 2008 2:58 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Re: JEOL SEM's: comments on 5000 series vs 6000 } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have had a JEOL 5800LV for almost 14 years. It gets a ton of work in } the VP mode and works very well. Imaging is limited to the backscattered } detector in the VP mode, and as Gary noted, resolution is not great at } 10K and greater magnification in VP mode. But this instrument performs } very well at high vacuum. I do not think that there is any decrease in } performance in high vacuum mode compared to a non-VP instrument. Imaging } at 50KX is possible and I have worked at voltages as low as 2 KV. Not } bad for a tungsten source. } } I have limited experience with the 6000 series instruments. I think that } the column and detectors were very similar if not identical to the 5800. } Some later instruments had greater Z travel than the 5800. The 5800 had } an archaic computer control system, whereas the 6000 series models had a } regular PC interface and hardware. (I think the 5900 may have had a PC } rather than the proprietary computer.) Our 5800 still works great, but I } am concerned about availability of parts as time goes on, especially for } the computer electronics. I am hopeful that we can keep our 5800 running } for a few more years, but we have had a couple of issues finding } components. } } We start installation of a new JEOL 6610 model next Monday. This } instrument has considerable improvements over previous models in the } 6000 series. The user interface is new with more imaging options and } better stage navigation. There is a new detector for VP mode that } provides imaging similar to secondary electron images. The signal to } noise on the BS detector is greatly improved and you can get great } backscatter images at very low KV. } } Hope this helps. } }
-- ~~~~~~~~~~~~~~~~~~~~ Bryan Bandli SEM Laboratory Manager University of Minnesota Duluth Life Science 93
Mail: UMD Geological Sciences 229 Heller Hall 1114 Kirby Dr. Duluth, MN 55812-3036
Phone: 218-726-7362 Fax: 218-726-8275
www.d.umn.edu/SEM/
==============================Original Headers============================== 14, 31 -- From bbandli-at-d.umn.edu Mon Dec 8 13:23:33 2008 14, 31 -- Received: from mx0.d.umn.edu (mx0.d.umn.edu [131.212.109.42]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8JNXsc011659 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:33 -0600 14, 31 -- Received: from mail.d.umn.edu (mail.d.umn.edu [131.212.109.2]) 14, 31 -- by mx0.d.umn.edu (8.13.8/8.13.8) with ESMTP id mB8JNXRk025521 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:33 -0600 (CST) 14, 31 -- Received: from mxv2.d.umn.edu (mxv2.d.umn.edu [131.212.109.136]) 14, 31 -- by mail.d.umn.edu (8.12.9/8.12.9) with ESMTP id mB8JNUPV002890 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:30 -0600 (CST) 14, 31 -- Received: from smtp.d.umn.edu (mx3.d.umn.edu [131.212.109.40]) 14, 31 -- by mxv2.d.umn.edu (8.13.8/8.13.8) with ESMTP id mB8JNUuw004913 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:30 -0600 (CST) 14, 31 -- Received: from [131.212.71.113] (nomad71-113.d.umn.edu [131.212.71.113]) 14, 31 -- (authenticated bits=0) 14, 31 -- by smtp.d.umn.edu (8.13.8/8.13.8) with ESMTP id mB8JMx5M011252 14, 31 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:04 -0600 (CST) 14, 31 -- Message-ID: {493D7414.7050300-at-d.umn.edu} 14, 31 -- Date: Mon, 08 Dec 2008 13:23:00 -0600 14, 31 -- From: Bryan Bandli {bbandli-at-d.umn.edu} 14, 31 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 14, 31 -- MIME-Version: 1.0 14, 31 -- To: Microscopy-at-microscopy.com 14, 31 -- Subject: Re: JEOL SEM's: comments on 5000 series vs 6000 14, 31 -- References: {200812072216.mB7MGnOv006169-at-ns.microscopy.com} 14, 31 -- In-Reply-To: {200812072216.mB7MGnOv006169-at-ns.microscopy.com} 14, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 31 -- Content-Transfer-Encoding: 7bit 14, 31 -- X-Virus-Scanned: ClamAV 0.94.2/8731/Sun Dec 7 22:31:15 2008 on mxv2.d.umn.edu 14, 31 -- X-Virus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jan.hejna-at-pwr.wroc.pl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jan.hejna-at-pwr.wroc.pl Name: Jan Hejna
Organization: Wroclaw University of Technology, Wroclaw, Poland
Title-Subject: [Filtered] Hitachi H800 goniometer
Question: Dear colleagues,
We have got the Hitachi H800 TEM from other university and we have some problems with assembling the side-entry goniometer. It was not used in previous location; they used a top-entry one. We have incomplete documentation and cannot find some parts. I would be very grateful when someone sent us copies of goniometer drawings. Drawings will help us in looking for missing parts or in making new ones.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both krekeler-at-uni-bonn.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Anorganische Chemie der Universitaet Bonn
Title-Subject: [Filtered] Passive Picture Acquisition on a JEOL6400F
Question: Hi,
recently our working group got a JEOL-6400F fitted with a Voyager EDX-System but also with a buggy vacuum system virtually for free! After some fiddling with new valves and new pumps the SEM works fine for now.
The last item on our To-Do list is: "Proper digital pictures"
Our Voyager System is only capable of 512*512 in Average or 1024*1024 in Live-Mode. The pictures are OK but lack the micron bar and the full 2000 lines resolution the SEM is capable of in Slowscan or Photoscan. We also have Problems to get the TIFF-Pictures ported to our Windows Systems, because of some network issues. Thus we want to tap the Video-Signal to the (unused) recording CRT for a passive acquisition of a High Resolution Picture via a Windows-System. A quick Search here resulted in one Hit, "Printerface" by GW-Electronics now EBSciences.
Does anyone has experience with that system or knows another cheap ( {2000$) and easy-to-use solution?
I am not familiar with the Voyager system, but I am a little surprised that it does no more than 1024x1024 imaging. Does it give a choice of dwell time per pixel?
We have active capture systems on both of our microscopes and a Quartz PCI passive system on one of them. Both active modes provide for quite a range of pixel densities and acquisition time.
Personally, I am usually happy with 1024-pixel-wide images. I guess you could get a 2048-pixel-wide image to display in its full resolution on some computer monitors. However, any down-scaling will sacrifice detail. I am not generally in favor of sampling at higher densities. Sure, there will be more pixels, but the time spent acquiring each pixel will be less resulting in more noise. I would rather use the microscopes optics to zoom in and take another image than to digitally zoom.
Noise is a critical issue. I try to drive the point home for our new users: there is a trade-off between resolution, signal quality (noise), and speed. Users must lower the beam current to improve resolution, but that will sacrifice signal-to-noise ratio. That can be recovered if the acquisition can be slowed down. If it can't, you don't want your signal spread out over too many pixels. If you seem to have great signal-to-noise ratio, then maybe the beam should be stopped down more to get even better resolution.
Passive and active systems SHOULD give the same performance for the same total acquisition time. However, you should check to be sure the results are equivalent. A lot will depend on the implementation. I am pretty happy with the comparison between the two on our one SEM, but your mileage may vary.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: krekeler-at-uni-bonn.de [mailto:krekeler-at-uni-bonn.de] Sent: Monday, December 08, 2008 3:26 PM To: wesaia-at-iastate.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both krekeler-at-uni-bonn.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Anorganische Chemie der Universitaet Bonn
Title-Subject: [Filtered] Passive Picture Acquisition on a JEOL6400F
Question: Hi,
recently our working group got a JEOL-6400F fitted with a Voyager EDX-System but also with a buggy vacuum system virtually for free! After some fiddling with new valves and new pumps the SEM works fine for now.
The last item on our To-Do list is: "Proper digital pictures"
Our Voyager System is only capable of 512*512 in Average or 1024*1024 in Live-Mode. The pictures are OK but lack the micron bar and the full 2000 lines resolution the SEM is capable of in Slowscan or Photoscan. We also have Problems to get the TIFF-Pictures ported to our Windows Systems, because of some network issues. Thus we want to tap the Video-Signal to the (unused) recording CRT for a passive acquisition of a High Resolution Picture via a Windows-System. A quick Search here resulted in one Hit, "Printerface" by GW-Electronics now EBSciences.
Does anyone has experience with that system or knows another cheap ( {2000$) and easy-to-use solution?
Is a small bottle of chloroform really something so deadly as to make one go so much out of one's way to avoid? I do not think so.
To clarify my position - yes, it is definitely dangerous for those with decreased sense of smell and is perhaps better used sparingly in teaching labs. Too many of young people just assume all those safety warnings are not for them (I did, remember that very well)... For an experienced EM/LM technologist or researcher, however, occasional use of chloroform is not a big deal, and it can save a lot of time and frustration. As long as you don't sniff the chloroform, this will (or at least should) be near the very bottom of the list of toxic stuff you get exposed, especially if you live in a big city and eat food from a supermarket.
OK, I did not realize there is more than one type of heat pen available. It is very possible that some will work better than the one I got. Should I test them all? varying distance, placement, etc? And think about that each time? Chloroform always works, and it gives you a piece of mind - just like propylene oxide does for embedding.
Let's just use common sense and not let safety officers make us paranoid.
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and not his employer. These opinions and experiences do not represent a consensus in the NIH scientific community. On the good side, this message is not confidential and can be freely shared and reproduced.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Gizelle } } I suppose I should make comment, as I did to your first enquiry. } } I have used Spurr's for many years and had few problems with } flattening silver/gold (80-100nm) sections on an ultramicrotome } cutting at 1-2mm/sec speed. I have always used a medium/hard mix of } Spurr's with few problems unless the embedding has been soft. } } I can think of several possible sources of the problem: } The heat pen that I use is a tungsten wire type which glows red/yellow } but certainly not white. Mine uses a fixed mains electric controller, } but some used to be available with a variable heat adjustment. } Another possible source of problems is the change of formulation of } Spurr's resin over the last year or so. It would be worth you trying } some much older Spurr's blocks if you are using the new Spurr's. } A final issue that sometimes arises in some environments is the build } up of static on the surface of sections which tends to make the } sections disperse when you approach with a heat pen or tweezers and } grid sometimes. Humidifiers or anti-static guns may solve this but it } would be important to establish that this is the problem before } spending more money. } } I hope this is of some help and will keep you away from the dreaded } chloroform. } } Malcolm } } Malcolm Haswell } Electron Microscope Unit } Faculty of Applied Sciences } University of Sunderland } SUNDERLAND } SR1 3SD } UK } email: malcolm.haswell-at-sunderland.ac.uk } } } ----- Original Message ----- } X-from: gw265-at-cam.ac.uk } Date: Friday, December 5, 2008 0:16 am } Subject: [Microscopy] viaWWW: Heat-pen vs Chloroform for straightening } sections } } } } } } } } } ------------------------------------------------------------------- } } --------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } AmericaTo Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserverOn-Line Help } } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------ } } ---------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } ------------------------------------------------------------------- } } -------- } } Remember this posting is most likely not from a Subscriber, so } } when replying } } please copy both gw265-at-cam.ac.uk as well as the MIcroscopy } } Listserver--------------------------------------------------------- } } ------------------ } } } } Email: gw265-at-cam.ac.uk } } Name: Giselle Walker } } } } Organization: University of Cambridge } } } } Title-Subject: [Filtered] Heat-pen vs Chloroform for straightening } } sections } } Question: Some weeks back I asked the list about alternatives to } } chloroform for straightening out thin resin sections for TEM. } } } } After an overwhelming response of "HEAT PEN!!!!!" I duly got one, } } sold by Fine Science Tools. The tip supposedly heats up to 1000 } } degrees, and is certainly hot enough to go white and deliver a } } good } } burn to skin. } } {http://www.finescience.ca/commerce/ccp4151-low-cost-cautery-kit- } } 18010-00m.htm} } } } } However I'm finding that it's nowhere near as effective as } } chloroform: my sections are still too wrinkled to use, unless I } } use } } chloroform as well. The sections are 30, 40 or 50nm thick and of } } single protistan cells in Spurr's resin. } } } } I've tried holding the heated tip as close as possible to the } } sections, a few at a time. All that happens is that the ribbon } } shoots } } away (as a whole) over the surface of the water bath. There's no } } discernible difference between holding the tip 5m, 5cm, or 5mm } } away } } from the sections. } } } } Clearly I'm doing something wrong. Ideas would be very welcome! } } } } I suppose the next alternative to chloroform is wet-resin } } embedding } } for 3 weeks before polymerisation, which I haven't tried yet. } } Given } } that this isn't always feasible, it would be good to get the heat- } } pen } } technique working. } } } } } } Thanks, } } } } Giselle } } } } Login Host: 131.111.8.96 } } ------------------------------------------------------------------- } } -------- } } } } ==============================Original } } Headers==============================14, 11 -- From } } zaluzec-at-microscopy.com Thu Dec 4 18:12:03 2008 } } 14, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } } [206.69.208.22])14, 11 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id mB50C2hB007160 } } 14, 11 -- for {microscopy-at-microscopy.com} ; Thu, 4 Dec 2008 } } 18:12:02 -0600 } } 14, 11 -- Mime-Version: 1.0 } } 14, 11 -- Message-Id: {p06240800c55e2242c536-at-[206.69.208.22]} } } 14, 11 -- Date: Thu, 4 Dec 2008 18:12:01 -0600 } } 14, 11 -- To: microscopy-at-microscopy.com } } 14, 11 -- From: gw265-at-cam.ac.uk (by way of MicroscopyListserver) } } 14, 11 -- Subject: viaWWW: Heat-pen vs Chloroform for } } straightening sections } } 14, 11 -- Content-Type: text/plain; charset="us-ascii" ; } } format="flowed"==============================End of - } } Headers============================== } } ==============================Original } Headers============================== } 10, 36 -- From malcolm.haswell-at-sunderland.ac.uk Fri Dec 5 04:01:08 } 2008 } 10, 36 -- Received: from max1.sunderland.ac.uk } (max1.sunderland.ac.uk [157.228.29.83]) } 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
I am currently re-tooling my ImageThief passive image capture system (free, open project) for a USB interface. The original version has the shortcomings that it was written for a DOS environment, used a $300 DMA board, was written entirely in assembly language and for the PC only. Information about that project and the general plan for the USB version is on my web page: http://people.umass.edu/dac/projects/ImageThief/ImageThief.html That page has links to some representative images.
It is designed for the JSM-5400 but the design is flexible and uses a horizontal blanking, vertical blanking and video signal. You can tweek the parameters for a different system. The pixel clock for the JEOL "high-resolution" mode is 18us/pixel and so any system with a similar frame timing should be adaptable; the pixel clock could probably be reduced a little for a 2k line resolution in a 50ms line.
I expect the little circuit boards for the new version will cost about $50 (this is a free, open project; I don't want to sell anything!) and easy to build; will plug into the computer via a USB cable. I'm close to getting the prototype assembled. I want to make it run under ImageJ or Micro-Manager so that it will be platform independent. I'm working alone on this and only know a bit of java, so any collaborations would be welcome and help get this out to the public.
As for how this would be implemented, I expect that many people could do the project themselves, and possibly vendors may be willing to set it up for those who need some help with it - as a "value-added" service, but I hope that the free design and code will keep costs reasonable for the ultimate users.
Let me know if you are interested.
Cheers!
Dale
krekeler-at-uni-bonn.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both krekeler-at-uni-bonn.de as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: krekeler-at-uni-bonn.de } Name: Tobias Krekeler } } Organization: Anorganische Chemie der Universitaet Bonn } } Title-Subject: [Filtered] Passive Picture Acquisition on a JEOL6400F } } Question: Hi, } } recently our working group got a JEOL-6400F } fitted with a Voyager EDX-System but also with a } buggy vacuum system virtually for free! After } some fiddling with new valves and new pumps the } SEM works fine for now. } } The last item on our To-Do list is: } "Proper digital pictures" } } Our Voyager System is only capable of 512*512 in } Average or 1024*1024 in Live-Mode. The pictures } are OK but lack the micron bar and the full 2000 } lines resolution the SEM is capable of in } Slowscan or Photoscan. We also have Problems to } get the TIFF-Pictures ported to our Windows } Systems, because of some network issues. Thus we } want to tap the Video-Signal to the (unused) } recording CRT for a passive acquisition of a High } Resolution Picture via a Windows-System. A quick } Search here resulted in one Hit, "Printerface" by } GW-Electronics now EBSciences. } } Does anyone has experience with that system or } knows another cheap ( {2000$) and easy-to-use } solution? } } Thanks, } } } Tobias } } Tobias Krekeler } Institut f¸r anorganische Chemie der Universitaet Bonn } Roemerstr. 164 } D-53117 Bonn } +49 (0)228 734180 } krekeler-at-uni-bonn.de } } Login Host: 131.220.128.75 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 15, 13 -- From zaluzec-at-microscopy.com Mon Dec 8 15:25:47 2008 } 15, 13 -- Received: from [10.158.144.177] (msdvpn8.msd.anl.gov [130.202.238.72]) } 15, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8LPdnd017599 } 15, 13 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 15:25:42 -0600 } 15, 13 -- Mime-Version: 1.0 } 15, 13 -- Message-Id: {p06240802c5634141af2f-at-[10.158.144.177]} } 15, 13 -- Date: Mon, 8 Dec 2008 22:25:38 +0100 } 15, 13 -- To: microscopy-at-microscopy.com } 15, 13 -- From: krekeler-at-uni-bonn.de (by way of MicroscopyListserver) } 15, 13 -- Subject: viaWWW: Passive Picture Acquisition on a JEOL6400F } 15, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 15, 13 -- Content-Transfer-Encoding: 8bit } 15, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB8LPdnd017599 } ==============================End of - Headers==============================
==============================Original Headers============================== 13, 20 -- From dac-at-research.umass.edu Mon Dec 8 17:27:15 2008 13, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8NRDO0002099 13, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 17:27:14 -0600 13, 20 -- Received: from [192.168.1.100] (static.unknown.charter.com [96.39.6.64] (may be forged)) 13, 20 -- (authenticated bits=0) 13, 20 -- by race1.oit.umass.edu (8.14.3/8.14.3) with ESMTP id mB8NRBZu003617 13, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 13, 20 -- Mon, 8 Dec 2008 18:27:12 -0500 13, 20 -- Message-ID: {493DAD6C.8080902-at-research.umass.edu} 13, 20 -- Date: Mon, 08 Dec 2008 18:27:40 -0500 13, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 13, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.18) Gecko/20081031 SeaMonkey/1.1.13 13, 20 -- MIME-Version: 1.0 13, 20 -- To: krekeler-at-uni-bonn.de, Microscopy List {microscopy-at-microscopy.com} 13, 20 -- Subject: Re: [Microscopy] viaWWW: Passive Picture Acquisition on a JEOL6400F 13, 20 -- References: {200812082130.mB8LUhD9000331-at-ns.microscopy.com} 13, 20 -- In-Reply-To: {200812082130.mB8LUhD9000331-at-ns.microscopy.com} 13, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
On Dec 8, 2008, at 3:22 PM, vladislav_speransky-at-nih.gov wrote:
} Is a small bottle of chloroform really something so deadly as to make } one go so much out of one's way to avoid? I do not think so. } } To clarify my position - yes, it is definitely dangerous for those } with decreased sense of smell and is perhaps better used sparingly in } teaching labs. Too many of young people just assume all those safety } warnings are not for them (I did, remember that very well)... For an } experienced EM/LM technologist or researcher, however, occasional use } of chloroform is not a big deal, and it can save a lot of time and } frustration. As long as you don't sniff the chloroform, this will (or } at least should) be near the very bottom of the list of toxic stuff } you get exposed, especially if you live in a big city and eat food } from a supermarket.
Dear Vlad, Chloroform is toxic to the liver, and it should definitely be handled in a hood. While it is not as toxic as CCl4, and notwithstanding that it was an ingredient in cough syrup at one time, it can cause liver cancer. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Mon Dec 8 17:49:24 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8NnOY8022083 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 17:49:24 -0600 6, 22 -- Received: from fire-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by fire-doxen-postvirus (Postfix) with ESMTP id 21FD8328995 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 15:49:24 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on fire-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by fire-doxen-ssl (Postfix) with ESMTP id 368853289F1 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 15:49:23 -0800 (PST) 6, 22 -- Message-Id: {2C85A72D-EC91-4CBF-8AC5-F06DB11828F5-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200812082322.mB8NM9MB027304-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Re: [Microscopy] Fwd: Re: viaWWW: Heat-pen vs Chloroform for straightening 6, 22 -- Date: Mon, 8 Dec 2008 15:49:22 -0800 6, 22 -- References: {200812082322.mB8NM9MB027304-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
All, We are in the planning stages to build a new MRI and fMRI facility in a new research building next door to our existing nano-characterization facility. I was wondering whether anyone has any data handy that bears on the question of how far away this new MRI lab needs to be located to avoid extraneous EMI fields and acoustic and mechanical vibration from impacting on the various SEM, FIB and TEM equipment already present.
I've done some googling but nothing useful has popped up. Has anyone been in a similar lab design/modification situation? What design factors were considered? What about shielding and/or mitigation?
Your data and/or experiences would be most welcome! john
John Donovan Director- Microanalytical Facility CAMCOR-UofO 541-346-4632 donovan-at-uoregon.edu www.epmalab.uoregon.edu
==============================Original Headers============================== 6, 19 -- From donovan-at-uoregon.edu Mon Dec 8 18:32:33 2008 6, 19 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB90WXlH003955 6, 19 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 18:32:33 -0600 6, 19 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 6, 19 -- (authenticated bits=0) 6, 19 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id mB90WWAj006016 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 19 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 16:32:33 -0800 6, 19 -- Message-Id: {200812090032.mB90WWAj006016-at-smtp.uoregon.edu} 6, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 6, 19 -- Date: Mon, 08 Dec 2008 16:32:47 -0800 6, 19 -- To: microscopy-at-microscopy.com 6, 19 -- From: John Donovan {donovan-at-uoregon.edu} 6, 19 -- Subject: e-beam friendly MRI facilities 6, 19 -- Mime-Version: 1.0 6, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 19 -- X-Virus-Scanned: ClamAV 0.94.2/8731/Sun Dec 7 20:31:15 2008 on mserv1 6, 19 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Well this one is new for us. We are getting some crayfish and fairy shrimp samples. They will be collected on site and sent to us in fixative. Does anyone have experience fixing and embedding them for TEM? I am concerned about osmotic issues and would appreciate any suggestions regarding this. I have checked a few references and am concerned about the quality of the fixation.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 8, 29 -- From dsherman-at-purdue.edu Mon Dec 8 21:56:31 2008 8, 29 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB93uVsV021310 8, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 21:56:31 -0600 8, 29 -- Received: from mailhub126.itcs.purdue.edu (mailhub126.itcs.purdue.edu [128.210.5.126]) 8, 29 -- by mailhub128.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id mB93uUgB021678 8, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 22:56:31 -0500 8, 29 -- Received: from 1061exfe03a.itap.purdue.edu (1061exfe03a.itap.purdue.edu [128.210.1.10]) 8, 29 -- by mailhub126.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id mB93uUYP029195 8, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 22:56:30 -0500 8, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe03a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 8, 29 -- Mon, 8 Dec 2008 22:56:30 -0500 8, 29 -- Received: from 98.228.28.11 ([98.228.28.11]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.104]) with Microsoft Exchange Server HTTP-DAV ; 8, 29 -- Tue, 9 Dec 2008 03:56:29 +0000 8, 29 -- User-Agent: Microsoft-Entourage/12.13.0.080930 8, 29 -- Date: Mon, 08 Dec 2008 22:56:29 -0500 8, 29 -- Subject: Fixing fairy shrimp 8, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 29 -- Message-ID: {C563569D.24817%dsherman-at-purdue.edu} 8, 29 -- Thread-Topic: Fixing fairy shrimp 8, 29 -- Thread-Index: AclZsh10asFZQBTTiUy9rkPctz57oA== 8, 29 -- Mime-version: 1.0 8, 29 -- Content-type: text/plain; 8, 29 -- charset="US-ASCII" 8, 29 -- Content-transfer-encoding: 7bit 8, 29 -- X-OriginalArrivalTime: 09 Dec 2008 03:56:30.0675 (UTC) FILETIME=[1E742A30:01C959B2] 8, 29 -- X-PMX-Version: 5.4.0.320885 8, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
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Title-Subject: [Filtered] electromagnetic Frequencies in water
Question: i would like to know if there is any evidence or tests that can be done that proves that water retains electromagnetic frequencies that have been introduced into the water? Also how does it work that the water retains those frequencies?
Reminds me of homeopathy water "memory" pseudo-science where it is claimed that after a 33X dilution (10^-33) the water somehow "remembers" the active ingredient! john
At 09:43 PM 12/8/2008, you wrote:
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==============================Original Headers============================== 8, 20 -- From donovan-at-uoregon.edu Tue Dec 9 00:11:22 2008 8, 20 -- Received: from QMTA09.emeryville.ca.mail.comcast.net (qmta09.emeryville.ca.mail.comcast.net [76.96.30.96]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB96BLsw018387 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 00:11:22 -0600 8, 20 -- Message-Id: {200812090611.mB96BLsw018387-at-ns.microscopy.com} 8, 20 -- Received: from OMTA05.emeryville.ca.mail.comcast.net ([76.96.30.43]) 8, 20 -- by QMTA09.emeryville.ca.mail.comcast.net with comcast 8, 20 -- id opMJ1a01b0vp7WLA9uBLZY; Tue, 09 Dec 2008 06:11:21 +0000 8, 20 -- Received: from SOURCE.uoregon.edu ([67.169.211.194]) 8, 20 -- by OMTA05.emeryville.ca.mail.comcast.net with comcast 8, 20 -- id ouBB1a0034CCeiB8RuBBTL; Tue, 09 Dec 2008 06:11:20 +0000 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 20 -- Date: Mon, 08 Dec 2008 22:11:11 -0800 8, 20 -- To: microscopy-at-microscopy.com 8, 20 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 8, 20 -- Subject: Re: [Microscopy] viaWWW: electromagnetic Frequencies in water 8, 20 -- In-Reply-To: {200812090543.mB95hP8i012006-at-ns.microscopy.com} 8, 20 -- References: {200812090543.mB95hP8i012006-at-ns.microscopy.com} 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Did I call it or what? Reading a bit further we find this hilarious page:
http://vavawater.com/research.htm
Quoting from it:
"Scientific explanations of Homeopathy are based on the idea that water is capable of "remembering" the frequencies emitted by the molecules that it has been exposed to. This is similar to the physical process of epitaxy used in material science where a substance takes on the same characteristics of a crystalline substrate it has been placed in contact with. However, Vava® does not add any substances but directly charges water with beneficial frequencies which are imprinted in the water and subsequently affect the body. Dr. Wolfgang Ludwig, a very distinguished German researcher, stated that, "Water has the memory of an elephant."
And a bit later on:
"Dr. Smith's work helps explain the ability of water clusters to absorb and emit electromagnetic fields that are comparable to the fields of the potentized molecules whose fingerprints they carry. Again, just as a water cluster can emit an electromagnetic frequency (EMF), so can it absorb frequencies. In other words, there must, according to the laws of physics, be a complementary relationship between the structures present in water and the fields it is exposed to, and vice versa. This explains how the therapeutic energy fields produced by electromagnetic frequencies can become incorporated into the structure of the water clusters in Vava® Water."
john
At 09:43 PM 12/8/2008, you wrote:
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==============================Original Headers============================== 14, 20 -- From donovan-at-uoregon.edu Tue Dec 9 00:32:14 2008 14, 20 -- Received: from QMTA05.emeryville.ca.mail.comcast.net (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB96WDB7001107 14, 20 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 00:32:13 -0600 14, 20 -- Message-Id: {200812090632.mB96WDB7001107-at-ns.microscopy.com} 14, 20 -- Received: from OMTA13.emeryville.ca.mail.comcast.net ([76.96.30.52]) 14, 20 -- by QMTA05.emeryville.ca.mail.comcast.net with comcast 14, 20 -- id ouRt1a00217UAYkA5uYCs1; Tue, 09 Dec 2008 06:32:12 +0000 14, 20 -- Received: from SOURCE.uoregon.edu ([67.169.211.194]) 14, 20 -- by OMTA13.emeryville.ca.mail.comcast.net with comcast 14, 20 -- id ouYB1a00E4CCeiB8ZuYCd0; Tue, 09 Dec 2008 06:32:12 +0000 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 20 -- Date: Mon, 08 Dec 2008 22:32:11 -0800 14, 20 -- To: microscopy-at-microscopy.com 14, 20 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 14, 20 -- Subject: Re: [Microscopy] viaWWW: electromagnetic Frequencies in water 14, 20 -- Mime-Version: 1.0 14, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 14, 20 -- Content-Transfer-Encoding: 8bit 14, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB96WDB7001107 ==============================End of - Headers==============================
Well, the Vava site may be a bit wawa, but water is weirder than you think.
This is a bit off-topic but interesting at the microscale - check out this video of Gerald Pollack, a biophysicist/engineer at U. Washington, giving a lecture about how water becomes structured at hydrophilic surfaces. http://www.researchchannel.org/mov/uw_fac_welife_1300k_qt.mov
Amazing images showing that a large clear zone develops next to such surfaces, from which all particles except water are excluded - this includes particles ranging in size from microns to a few Daltons (the latter = pH indicator dye). It's been looked at before - review published in 1949, but Pollack has brought this back into circulation and extended it.
cheers, Rosemary
On 9/12/08 5:36 PM, "donovan-at-uoregon.edu" {donovan-at-uoregon.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Did I call it or what? Reading a bit further we find this hilarious page: } } http://vavawater.com/research.htm } } Quoting from it: } } "Scientific explanations of Homeopathy are based } on the idea that water is capable of } "remembering" the frequencies emitted by the } molecules that it has been exposed to. This is } similar to the physical process of epitaxy used } in material science where a substance takes on } the same characteristics of a crystalline } substrate it has been placed in contact } with. However, Vava® does not add any substances } but directly charges water with beneficial } frequencies which are imprinted in the water and } subsequently affect the body. Dr. Wolfgang } Ludwig, a very distinguished German researcher, } stated that, "Water has the memory of an elephant." } } And a bit later on: } } "Dr. Smith's work helps explain the ability of } water clusters to absorb and emit electromagnetic } fields that are comparable to the fields of the } potentized molecules whose fingerprints they } carry. Again, just as a water cluster can emit an } electromagnetic frequency (EMF), so can it absorb } frequencies. In other words, there must, } according to the laws of physics, be a } complementary relationship between the structures } present in water and the fields it is exposed to, } and vice versa. This explains how the therapeutic } energy fields produced by electromagnetic } frequencies can become incorporated into the } structure of the water clusters in Vava® Water." } } john } } } At 09:43 PM 12/8/2008, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both lcb-at-vavawater.com as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: lcb-at-vavawater.com } } Name: Lauren Clarke-Bennett } } } } Organization: Vava Now Inc. } } } } Title-Subject: [Filtered] electromagnetic Frequencies in water } } } } Question: i would like to know if there is any evidence or tests } } that can be done that proves that water retains electromagnetic } } frequencies that have been introduced into the water? Also how does } } it work that the water retains those frequencies? } } } } Login Host: 68.161.80.112 } } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } } 6, 11 -- From zaluzec-at-microscopy.com Mon Dec 8 23:39:28 2008 } } 6, 11 -- Received: from [10.158.144.177] } } (msdvpn8.msd.anl.gov [130.202.238.72]) } } 6, 11 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id mB95dPbl004366 } } 6, 11 -- for {microscopy-at-microscopy.com} ; } } Mon, 8 Dec 2008 23:39:27 -0600 } } 6, 11 -- Mime-Version: 1.0 } } 6, 11 -- Message-Id: {p06240806c563b4c3c353-at-[10.158.144.177]} } } 6, 11 -- Date: Tue, 9 Dec 2008 06:39:24 +0100 } } 6, 11 -- To: microscopy-at-microscopy.com } } 6, 11 -- From: lcb-at-vavawater.com (by way of MicroscopyListserver) } } 6, 11 -- Subject: viaWWW: electromagnetic Frequencies in water } } 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 14, 20 -- From donovan-at-uoregon.edu Tue Dec 9 00:32:14 2008 } 14, 20 -- Received: from QMTA05.emeryville.ca.mail.comcast.net } (qmta05.emeryville.ca.mail.comcast.net [76.96.30.48]) } 14, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mB96WDB7001107 } 14, 20 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 00:32:13 -0600 } 14, 20 -- Message-Id: {200812090632.mB96WDB7001107-at-ns.microscopy.com} } 14, 20 -- Received: from OMTA13.emeryville.ca.mail.comcast.net ([76.96.30.52]) } 14, 20 -- by QMTA05.emeryville.ca.mail.comcast.net with comcast } 14, 20 -- id ouRt1a00217UAYkA5uYCs1; Tue, 09 Dec 2008 06:32:12 +0000 } 14, 20 -- Received: from SOURCE.uoregon.edu ([67.169.211.194]) } 14, 20 -- by OMTA13.emeryville.ca.mail.comcast.net with comcast } 14, 20 -- id ouYB1a00E4CCeiB8ZuYCd0; Tue, 09 Dec 2008 06:32:12 +0000 } 14, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 14, 20 -- Date: Mon, 08 Dec 2008 22:32:11 -0800 } 14, 20 -- To: microscopy-at-microscopy.com } 14, 20 -- From: "John J. Donovan" {donovan-at-uoregon.edu} } 14, 20 -- Subject: Re: [Microscopy] viaWWW: electromagnetic Frequencies in } water } 14, 20 -- Mime-Version: 1.0 } 14, 20 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed } 14, 20 -- Content-Transfer-Encoding: 8bit } 14, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mB96WDB7001107 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 45 -- From prvs=Rosemary.White=222545661-at-csiro.au Tue Dec 9 00:56:59 2008 9, 45 -- Received: from act-MTAout5.csiro.au (act-MTAout5.csiro.au [150.229.7.42]) 9, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB96uvOZ018224 9, 45 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 00:56:58 -0600 9, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 9, 45 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 9, 45 -- s=email; t=1228805819; x=1260341819; 9, 45 -- h=from:sender:reply-to:subject:date:message-id:to:cc: 9, 45 -- mime-version:content-transfer-encoding:content-id: 9, 45 -- content-description:resent-date:resent-from:resent-sender: 9, 45 -- resent-to:resent-cc:resent-message-id:in-reply-to: 9, 45 -- references:list-id:list-help:list-unsubscribe: 9, 45 -- list-subscribe:list-post:list-owner:list-archive; 9, 45 -- z=From:=20Rosemary=20White=20 {rosemary.white-at-csiro.au} 9, 45 -- |Subject:=20Re:=20[Microscopy]=20Re:=20viaWWW:=20electrom 9, 45 -- agnetic=20Frequencies=20in=20water|Date:=20Tue,=209=20Dec 9, 45 -- =202008=2017:56:48=20+1100|Message-ID:=20 {C56461E0.1EBF%r 9, 45 -- osemary.white-at-csiro.au} |To:=20 {microscopy-at-microscopy.com} 9, 45 -- |MIME-Version:=201.0|Content-Transfer-Encoding:=20quoted- 9, 45 -- printable|In-Reply-To:=20 {200812090636.mB96aEJC011031-at-ns. 9, 45 -- microscopy.com} ; 9, 45 -- bh=7KczzS3rhfhGdeB4Cwv3bCTgXTX6fo4nZui2F27/MWQ=; 9, 45 -- b=mJHLPgK77542dxccUp7PszSq5Hywrd/5BCBs52aGPW0pr+s09cjHFzVx 9, 45 -- pwCx7/MFX6xuFeHwRBeolNmB6/gzcqRqX0Qnit3UL7XZSUc+GZSpxlw3W 9, 45 -- IpLSFAgsHXpOILC; 9, 45 -- X-IronPort-AV: E=Sophos;i="4.33,739,1220191200"; 9, 45 -- d="scan'208";a="16021280" 9, 45 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 9, 45 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 09 Dec 2008 17:56:56 +1100 9, 45 -- Received: from [152.83.167.123] (152.83.167.123) by 9, 45 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server id 9, 45 -- 8.1.311.2; Tue, 9 Dec 2008 17:56:50 +1100 9, 45 -- User-Agent: Microsoft-Entourage/12.10.0.080409 9, 45 -- Date: Tue, 9 Dec 2008 17:56:48 +1100 9, 45 -- Subject: Re: [Microscopy] Re: viaWWW: electromagnetic Frequencies in water 9, 45 -- From: Rosemary White {rosemary.white-at-csiro.au} 9, 45 -- To: {microscopy-at-microscopy.com} 9, 45 -- Message-ID: {C56461E0.1EBF%rosemary.white-at-csiro.au} 9, 45 -- Thread-Topic: [Microscopy] Re: viaWWW: electromagnetic Frequencies in water 9, 45 -- Thread-Index: AclZyHI8a4bGg6PlSKaSxHzDeaXcdAAAtvbU 9, 45 -- In-Reply-To: {200812090636.mB96aEJC011031-at-ns.microscopy.com} 9, 45 -- MIME-Version: 1.0 9, 45 -- Content-Type: text/plain; charset="ISO-8859-1" 9, 45 -- Content-Transfer-Encoding: 8bit 9, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB96uvOZ018224 ==============================End of - Headers==============================
Very interesting talk! Don't get me wrong- water is truly weird and wonderful stuff. For one thing, you can't make Hollandaise sauce without it!
Unfortunately I expect poor Gerald will soon be quoted "generously" on the Vava wawa site. john
At 11:01 PM 12/8/2008, you wrote:
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==============================Original Headers============================== 8, 22 -- From donovan-at-uoregon.edu Tue Dec 9 01:56:49 2008 8, 22 -- Received: from QMTA01.emeryville.ca.mail.comcast.net (qmta01.emeryville.ca.mail.comcast.net [76.96.30.16]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB97umss000568 8, 22 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 01:56:49 -0600 8, 22 -- Message-Id: {200812090756.mB97umss000568-at-ns.microscopy.com} 8, 22 -- Received: from OMTA14.emeryville.ca.mail.comcast.net ([76.96.30.60]) 8, 22 -- by QMTA01.emeryville.ca.mail.comcast.net with comcast 8, 22 -- id ovvE1a0061HpZEsA1vwnjR; Tue, 09 Dec 2008 07:56:48 +0000 8, 22 -- Received: from SOURCE.uoregon.edu ([67.169.211.194]) 8, 22 -- by OMTA14.emeryville.ca.mail.comcast.net with comcast 8, 22 -- id ovwm1a00A4CCeiB8avwnrp; Tue, 09 Dec 2008 07:56:47 +0000 8, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 22 -- Date: Mon, 08 Dec 2008 23:52:09 -0800 8, 22 -- To: microscopy-at-microscopy.com 8, 22 -- From: "John J. Donovan" {donovan-at-uoregon.edu} 8, 22 -- Subject: Re: [Microscopy] viaWWW: electromagnetic Frequencies in water 8, 22 -- In-Reply-To: {200812090701.mB971kXB025865-at-ns.microscopy.com} 8, 22 -- References: {200812090701.mB971kXB025865-at-ns.microscopy.com} 8, 22 -- Mime-Version: 1.0 8, 22 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 8, 22 -- Content-Transfer-Encoding: 8bit 8, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB97umss000568 ==============================End of - Headers==============================
EDX should work provided that the signal is strong enough. In TEM sensitivity issue is a reality, but given that you can reasonably expect to have agglomerates of particles and that your sections will be pretty thick (for tomography), I think that it should work. BUT: really an artificial particle of high density should be recognizable from a biological material. Not only due to its density, but also due to its regularity in shape and sharpness of the border. Otherwise labeling using colloidal gold particles couldn't have been developed!!! Now if you think that the difference in contrast with your material is not strong enough, you can always use a weaker contrasting, or no contrasting at all! However I am pretty confident that you will quickly learn to recognize your nanoparticles without any other detectors than your eyes.
Best regards, Stephane
----- Original Message ---- X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} To: nizets2-at-yahoo.com Sent: Monday, December 8, 2008 3:19:49 PM
I would like to explore whether utilizing energy filtering or EDX mapping would be helpful to definitively identify locations of iron oxide nanoparticles ( also Cd or Se in quantum dot nanoprobes) in cells. These particles are often of similar size to cell components (such as ribosomes) and often density alone is not sufficient to identify them.
My understanding is that energy filtering gives best results with lighter elements while EDX can be superior with heavy elements. These are sort of in between so I do not know which technique would be preferable. We ultimately would like to do tomography to determine distribution of the nanoparticles in relation to cell organelles/components.
I would appreciate hearing from those with personal experience in dealing with like particles or referrals to pertinent articles.
Thanks, Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.agriculture.purdue.edu/microscopy/
==============================Original Headers============================== 6, 29 -- From dsherman-at-purdue.edu Mon Dec 8 08:14:04 2008 6, 29 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8EE4Qe000340 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 08:14:04 -0600 6, 29 -- Received: from mailhub127.itcs.purdue.edu (mailhub127.itcs.purdue.edu [128.210.5.127]) 6, 29 -- by mailhub131.itcs.purdue.edu (8.14.2/8.14.2/smtp-nopmx) with ESMTP id mB8EE3vc031862 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:14:04 -0500 6, 29 -- Received: from 1061exfe02a.itap.purdue.edu (1061exfe02a.itap.purdue.edu [128.210.1.9]) 6, 29 -- by mailhub127.itcs.purdue.edu (8.14.2/8.14.2/exchange-outbound) with ESMTP id mB8EE3Bm032288 6, 29 -- for {microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 09:14:03 -0500 6, 29 -- Received: from exch04.purdue.lcl ([172.21.6.24]) by 1061exfe02a.itap.purdue.edu with Microsoft SMTPSVC(6.0.3790.3959); 6, 29 -- Mon, 8 Dec 2008 09:14:03 -0500 6, 29 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH04.purdue.lcl ([172.21.6.26]) via Exchange Front-End Server exchange.purdue.edu ([128.210.1.9]) with Microsoft Exchange Server HTTP-DAV ; 6, 29 -- Mon, 8 Dec 2008 14:14:02 +0000 6, 29 -- User-Agent: Microsoft-Entourage/12.14.0.081024 6, 29 -- Date: Mon, 08 Dec 2008 09:14:02 -0500 6, 29 -- Subject: TEM-Localizing FE-NP using EF or EDX? 6, 29 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 29 -- Message-ID: {C56295DA.36FDB%dsherman-at-exchange.purdue.edu} 6, 29 -- Thread-Topic: TEM-Localizing FE-NP using EF or EDX? 6, 29 -- Thread-Index: AclZPzhZ78eIkKiyQCKgzk401KyKQQ== 6, 29 -- Mime-version: 1.0 6, 29 -- Content-type: text/plain; 6, 29 -- charset="US-ASCII" 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-OriginalArrivalTime: 08 Dec 2008 14:14:03.0681 (UTC) FILETIME=[395A7110:01C9593F] 6, 29 -- X-PMX-Version: 5.4.0.320885 6, 29 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
==============================Original Headers============================== 22, 23 -- From nizets2-at-yahoo.com Tue Dec 9 03:42:38 2008 22, 23 -- Received: from web110802.mail.gq1.yahoo.com (web110802.mail.gq1.yahoo.com [67.195.13.225]) 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mB99gcxo022100 22, 23 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 03:42:38 -0600 22, 23 -- Received: (qmail 58148 invoked by uid 60001); 9 Dec 2008 09:42:37 -0000 22, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 22, 23 -- s=s1024; d=yahoo.com; 22, 23 -- h=X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 22, 23 -- b=ZPWrlu8USw5Lg3/OdodcmU2fYPuUAWOekHDOigdM/1Q4Yc2yoztA29JzFA02QRKqzDEWM6ZcIT+3aJ2VIQb/Bm6C+rDiKPNUiC2DkzCqHx8Qs0tNVNkCv6/vLcg4po6uroivSckVsiEKUSjYwAt2JqEL040Yju8AH3RgKkLIA7U=; 22, 23 -- X-YMail-OSG: DkDlYzIVM1n2m4sfijt5OkHntBKFRkDJ_DgF.Pbrq.ZQTqUkFKy.m5d2gHAXgSdHKCwoSFMdXAmTJ4xl9FEULaqYCuqh4ME6ZvTaDjWmB2YEtgrehKJ65bGms3Ih2rOaBpvURxJxFb1VVo4w1LjdKBc8sTmZfgY41xxZA976XnO.68mj7UUQYqFES.6Si4NY9oowo0vSFYNCnnTUl.2p_nN6eKKeBlAo2QlgmIf3vZCXXhtTeRVqq42akkK2BxGDuiO.iRFa 22, 23 -- Received: from [80.122.101.100] by web110802.mail.gq1.yahoo.com via HTTP; Tue, 09 Dec 2008 01:42:37 PST 22, 23 -- X-Mailer: YahooMailRC/1155.32 YahooMailWebService/0.7.260.1 22, 23 -- References: {200812081419.mB8EJnQ5008155-at-ns.microscopy.com} 22, 23 -- Date: Tue, 9 Dec 2008 01:42:37 -0800 (PST) 22, 23 -- From: Stephane Nizet {nizets2-at-yahoo.com} 22, 23 -- Subject: Re: [Microscopy] TEM-Localizing FE-NP using EF or EDX? 22, 23 -- To: dsherman-at-purdue.edu 22, 23 -- Cc: microscopy-at-microscopy.com 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; charset=iso-8859-1 22, 23 -- Message-ID: {797428.57541.qm-at-web110802.mail.gq1.yahoo.com} 22, 23 -- Content-Transfer-Encoding: 8bit 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB99gcxo022100 ==============================End of - Headers==============================
Vlad: } Is a small bottle of chloroform really something so deadly as to make } one go so much out of one's way to avoid? I do not think so.
Bill:} Chloroform is toxic to the liver, and it should definitely be handled
} in a hood. While it is not as toxic as CCl4, and notwithstanding that } it was an ingredient in cough syrup at one time, it can cause liver } cancer.
My 2 cents: There can be little doubt that unnecessary exposure to chemicals can be potentially harmful. As we (scientist) discover the potential danger we must remember to balance that against the potential benefit. We don't want to take unnecessary risks, but I am reminded of the fury over benzene in gasoline. The initial regulation restricted benzene to no detectable amount and when instrumentation became sensitive enough it was found that just about everything seems to contain some incredibly small amount of benzene. I'm not sure the exposure to a part per trillion of benzene for 5 minutes while filling up your car is significant. Nor am I convinced that drop of chloroform evaporating on a microscope slide while re-crystalizing an organic compound is significant. The same applies, I suspect to straightening a section.
We (that's us scientist) look back on the last 400 years of science and it's hard to believe we don't have a understanding of everything. I suspect the science of toxicology will have many interesting new discoveries as genetics unravel why, for example, some people smoke for 60 years and die from non lung related complications and others develop cancer from 10 years of second hand smoke.
Lets not let the concern for reasonable safety become so overwhelming as to hobble science.
I end with my traditional fairwell: Stay safe.....
Frank Karl....
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==============================Original Headers============================== 16, 23 -- From frank_karl-at-lincolnelectric.com Tue Dec 9 06:12:25 2008 16, 23 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 16, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9CCOde007349 16, 23 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 06:12:24 -0600 16, 23 -- In-Reply-To: {200812082354.mB8NsWEk002541-at-ns.microscopy.com} 16, 23 -- Subject: Re: [Microscopy] Re: Fwd: Re: viaWWW: Heat-pen vs Chloroform for 16, 23 -- straightening 16, 23 -- To: Microscopy-at-microscopy.com 16, 23 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 16, 23 -- Message-ID: {OF82533403.8C02CDB5-ON8525751A.0040A5A6-8525751A.00430427-at-lincolnelectric.com} 16, 23 -- Date: Tue, 9 Dec 2008 07:12:03 -0500 16, 23 -- From: Frank_Karl-at-lincolnelectric.com 16, 23 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 16, 23 -- 07, 2008) at 12/09/2008 07:12:03 AM, 16, 23 -- CD-MIME complete at 12/09/2008 07:12:03 AM, 16, 23 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 16, 23 -- 07, 2008) at 12/09/2008 07:12:03 AM, 16, 23 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 16, 23 -- 07, 2008) at 12/09/2008 07:12:03 AM, 16, 23 -- Serialize complete at 12/09/2008 07:12:03 AM 16, 23 -- MIME-Version: 1.0 16, 23 -- Content-Type: text/plain; 16, 23 -- charset="US-ASCII" ==============================End of - Headers==============================
I agree in part with what Frank says, but would rather run the risk of an occasional trace of chloroform left on something removed from a fume-hood than a daily exposure to a known hazard in a small room. I would also be extremely concerned about exposing students or young visitors to such a harmful solvent and the ambiguities of saying something is harmful then using it in front of them ... just think of the potential legal implications.
To continue the rant we are now benefiting from many years of uncertainty about asbestos and cigarette smoke. The death tolls from both are set to rise for at least a decade or two more in the Western world.
Sermon over.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Frank_Karl-at-lincolnelectric.com
Debby,
What sort of limitations do you have in what you can do to the critters? The biggest problem getting good fixation is the cuticle. The simplest solution is to create holes: pull off appendages, cut off the ends of the appendages, and so on. The fixatives themselves are pretty routine, I've used Karnovsky's without issue. The problem is likely to be buffers. For aquatic critters, I generally use 0.2µm-filtered water taken from the collection site as the buffer solution. This may or not not work here, though. The crayfish I suspect are likely hypertonic to the freshwater, but! the physiologists have a Crustacean Ringers saline solution -- I'd use that for the buffer. It was developed initially for crayfish. Sorry, I don't have the recipe to hand. For the Artemia, ... if they're in brackish or sea-water type salt water, I'd filter that for the buffer. If they're from a hypersaline environment, I'd either try the Crustacean Riingers, or delve into the fairy-shrimp physiology literature for a physiological saline. Note: there are some folks on the crust-l mailing list that. You can get to it from the moderator's web page, Jeffrey Shields at Virginia Inst. Mar. Sci. http://www.vims.edu/~jeff/
Phil
} Well this one is new for us. We are getting some crayfish and fairy shrimp } samples. They will be collected on site and sent to us in fixative. Does } anyone have experience fixing and embedding them for TEM? I am concerned } about osmotic issues and would appreciate any suggestions regarding this. I } have checked a few references and am concerned about the quality of the } fixation. } } Debby } } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.agriculture.purdue.edu/microscopy -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 28 -- From oshel1pe-at-cmich.edu Tue Dec 9 07:58:03 2008 5, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9Dw3Sp006954 5, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 07:58:03 -0600 5, 28 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 28 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mB9Dvhgk028343; 5, 28 -- Tue, 9 Dec 2008 08:58:02 -0500 5, 28 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 5, 28 -- Tue, 9 Dec 2008 08:57:46 -0500 5, 28 -- Mime-Version: 1.0 5, 28 -- Message-Id: {f06240804c5642671275c-at-[141.209.160.249]} 5, 28 -- In-Reply-To: {200812090400.mB940dbS027230-at-ns.microscopy.com} 5, 28 -- References: {200812090400.mB940dbS027230-at-ns.microscopy.com} 5, 28 -- Date: Tue, 9 Dec 2008 08:57:39 -0500 5, 28 -- To: dsherman-at-purdue.edu 5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 28 -- Subject: Re: [Microscopy] Fixing fairy shrimp 5, 28 -- Cc: Microscopy-at-microscopy.com 5, 28 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 5, 28 -- X-OriginalArrivalTime: 09 Dec 2008 13:57:46.0783 (UTC) FILETIME=[1D7D62F0:01C95A06] 5, 28 -- X-Canit-CHI2: 0.00 5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 28 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 5, 28 -- X-CanItPRO-Stream: default 5, 28 -- X-Canit-Stats-ID: 6388385 - f5a1c6933d86 5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB9Dw3Sp006954 ==============================End of - Headers==============================
Microscopists, As we think about chloroform, I think it is worth remembering the ol' saw: the dose makes the poison. This seems clearly true with respect to cancer. The classic cancer sufferers are smokers, uranium miners, refinery workers; folks who had wholesale exposure day after day, month after month, year after year. Things are classified as carcinogens when they come up positive in animal tests but here too the agent is administered at a threshold cytotoxic dose (far greater than typical environmental doses) and thus there is a background of induced proliferation (due to repair) along with the massive dose. One third (roughly) of synthetic compounds turn up as carcinogens in animal tests. Natural compounds are rarely tested (no one to pay) but there is no reason to suppose the ratio would be different. We are exposed to natural compounds in our diet, many of them in high concentrations. The idea that exposure to trace chemical residues can give us cancer needs to be taken with more than a grain of salt. Following this logic, if you are a pathology lab where you cut and strech sections every day of the year, get a heat pen. But if your section stretching happens a few days a year, save energy, use the solvent.
My few molecules, Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Biology Department /_ / __ /__ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 / / ___ / \ \__/ \ ____ www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 2, 20 -- From baskin-at-bio.umass.edu Tue Dec 9 08:18:20 2008 2, 20 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 2, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9EIKRs021877 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 08:18:20 -0600 2, 20 -- Received: from [192.168.24.51] (aic22-p141.flets.hi-ho.ne.jp [219.126.167.141]) 2, 20 -- (authenticated bits=0) 2, 20 -- by marlin.bio.umass.edu (8.14.2/8.14.2) with ESMTP id mB9EIGYr014047 2, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 2, 20 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 09:18:19 -0500 (EST) 2, 20 -- Mime-Version: 1.0 2, 20 -- Message-Id: {p0624051ec5642a4f26d0-at-[192.168.24.51]} 2, 20 -- In-Reply-To: {200812091240.mB9Ce1aW022475-at-ns.microscopy.com} 2, 20 -- References: {200812091240.mB9Ce1aW022475-at-ns.microscopy.com} 2, 20 -- Date: Tue, 9 Dec 2008 23:18:14 +0900 2, 20 -- To: microscopy-at-microscopy.com 2, 20 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 2, 20 -- Subject: chloroform and cancer 2, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 20 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.0 (marlin.bio.umass.edu [128.119.55.19]); Tue, 09 Dec 2008 09:18:20 -0500 (EST) 2, 20 -- X-Scanned-By: MIMEDefang 2.54 on 128.119.55.19 ==============================End of - Headers==============================
There was a small animal MRI set up down the hall from my TEM and SEM for several months. I discussed with those installing the machine the concerns that I had about fields affecting my scopes and they assured me that there should be no problems.
Happily I can report that there were none. I do not know about the full sized ones that are your specific concern. Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} All, } We are in the planning stages to build a new MRI and fMRI facility in } a new research building next door to our existing } nano-characterization facility. I was wondering whether anyone has } any data handy that bears on the question of how far away this new } MRI lab needs to be located to avoid extraneous EMI fields and } acoustic and mechanical vibration from impacting on the various SEM, } FIB and TEM equipment already present. } } I've done some googling but nothing useful has popped up. Has anyone } been in a similar lab design/modification situation? What design } factors were considered? What about shielding and/or mitigation? } } Your data and/or experiences would be most welcome! } } John Donovan } Director- Microanalytical Facility } CAMCOR-UofO } 541-346-4632 } donovan-at-uoregon.edu } www.epmalab.uoregon.edu
==============================Original Headers============================== 8, 27 -- From connellyps-at-nhlbi.nih.gov Tue Dec 9 09:39:36 2008 8, 27 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9Fdabm005206 8, 27 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 09:39:36 -0600 8, 27 -- X-IronPortListener: Outbound_SMTP 8, 27 -- Received: from nihcessmtp3.hub.nih.gov ([128.231.90.117]) 8, 27 -- by nihxway2out.hub.nih.gov with ESMTP; 09 Dec 2008 10:38:29 -0500 8, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 8, 27 -- Tue, 9 Dec 2008 10:38:25 -0500 8, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.163]) with Microsoft Exchange Server HTTP-DAV ; 8, 27 -- Tue, 9 Dec 2008 15:38:24 +0000 8, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 8, 27 -- Date: Tue, 09 Dec 2008 10:37:52 -0500 8, 27 -- Subject: Re: [Microscopy] e-beam friendly MRI facilities 8, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 8, 27 -- To: {donovan-at-uoregon.edu} , 8, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 27 -- Message-ID: {C563FB00.2AF2%connellyps-at-nhlbi.nih.gov} 8, 27 -- Thread-Topic: [Microscopy] e-beam friendly MRI facilities 8, 27 -- Thread-Index: AclaFBjgV3DRCsYHEd2mtAANk2Yv1A== 8, 27 -- In-Reply-To: {200812090036.mB90a9c5011404-at-ns.microscopy.com} 8, 27 -- Mime-version: 1.0 8, 27 -- Content-type: text/plain; 8, 27 -- charset="ISO-8859-1" 8, 27 -- X-OriginalArrivalTime: 09 Dec 2008 15:38:25.0201 (UTC) FILETIME=[2CAADA10:01C95A14] 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mB9Fdabm005206 ==============================End of - Headers==============================
Hello Everyone, I need some advise on polishing cross-sections of electrical contacts. I'm looking at a PCB with a copper contact with a plating of nickel followed by a thin plating of gold. The gold plating reminds me of those watches you saw on TV years ago proclaiming "Generously plated with 1 micron of 24 carat gold... "
I'm carbon coating my samples and examining with the SEM
I've mounting the circuit board in a edge retention thermoplastic, but I still think I am smearing out my gold layer. Any ideas on retaining a thin layer of gold while grinding and polishing to 1um would be welcome.
stay safe........ Frank Karl.......
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 8, 21 -- From frank_karl-at-lincolnelectric.com Tue Dec 9 12:09:22 2008 8, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9I9MZk028821 8, 21 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 12:09:22 -0600 8, 21 -- Subject: edge retention of thin gold plating 8, 21 -- To: Microscopy-at-microscopy.com 8, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 8, 21 -- Message-ID: {OF5CF7D493.8FA73670-ON8525751A.0062DD0A-8525751A.0063B09A-at-lincolnelectric.com} 8, 21 -- Date: Tue, 9 Dec 2008 13:08:56 -0500 8, 21 -- From: Frank_Karl-at-lincolnelectric.com 8, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, 8, 21 -- CD-MIME complete at 12/09/2008 01:08:53 PM, 8, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, 8, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, 8, 21 -- Serialize complete at 12/09/2008 01:08:53 PM 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain; 8, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
Have you tried electroless nickel plating, then mounting etc.?
I haven't done that with thin gold plating but I have done it with a number of other thin coatings and it works quite well.
dj
On Tue, 9 Dec 2008, Frank_Karl-at-lincolnelectric.com wrote:
} Hello Everyone, } I need some advise on polishing cross-sections of electrical contacts. I'm } looking at a PCB with a copper contact with a plating of nickel followed by } a thin plating of gold. The gold plating reminds me of those watches you } saw on TV years ago proclaiming "Generously plated with 1 micron of 24 } carat gold... " } } I'm carbon coating my samples and examining with the SEM } } I've mounting the circuit board in a edge retention thermoplastic, but I } still think I am smearing out my gold layer. Any ideas on retaining a thin } layer of gold while grinding and polishing to 1um would be welcome. } } stay safe........ } Frank Karl....... } } } -- } ************************************************************* } Note: } The information contained in this message may be } privileged and confidential and protected from disclosure. If } the reader of this message is not the intended recipient, or } an employee or agent responsible for delivering this message } to the intended recipient, you are hereby notified that any } dissemination, distribution or copying of this communication } is strictly prohibited. If you have received this } communication in error, please notify us immediately by } replying to the message and deleting it from your computer. } Thank you, } The Lincoln Electric Company } ************************************************************** } } } ==============================Original Headers============================== } 8, 21 -- From frank_karl-at-lincolnelectric.com Tue Dec 9 12:09:22 2008 } 8, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9I9MZk028821 } 8, 21 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 12:09:22 -0600 } 8, 21 -- Subject: edge retention of thin gold plating } 8, 21 -- To: Microscopy-at-microscopy.com } 8, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 } 8, 21 -- Message-ID: {OF5CF7D493.8FA73670-ON8525751A.0062DD0A-8525751A.0063B09A-at-lincolnelectric.com} } 8, 21 -- Date: Tue, 9 Dec 2008 13:08:56 -0500 } 8, 21 -- From: Frank_Karl-at-lincolnelectric.com } 8, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February } 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, } 8, 21 -- CD-MIME complete at 12/09/2008 01:08:53 PM, } 8, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February } 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, } 8, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February } 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, } 8, 21 -- Serialize complete at 12/09/2008 01:08:53 PM } 8, 21 -- MIME-Version: 1.0 } 8, 21 -- Content-Type: text/plain; } 8, 21 -- charset="US-ASCII" } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 19 -- From dljones-at-bestweb.net Tue Dec 9 12:19:05 2008 6, 19 -- Received: from smtp3.bestweb.net (smtp3.bestweb.net [209.94.103.43]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9IJ4w1010010 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 12:19:05 -0600 6, 19 -- Received: from monet.bestweb.net (monet.bestweb.net [209.94.121.202]) 6, 19 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 19 -- (No client certificate requested) 6, 19 -- by smtp3.bestweb.net (Postfix) with ESMTPS id 572035C7A; 6, 19 -- Tue, 9 Dec 2008 13:19:02 -0500 (EST) 6, 19 -- Date: Tue, 9 Dec 2008 17:58:39 +0000 (UTC) 6, 19 -- From: dljones {dljones-at-bestweb.net} 6, 19 -- To: Frank_Karl-at-lincolnelectric.com 6, 19 -- cc: microscopy-at-microscopy.com 6, 19 -- Subject: Re: [Microscopy] edge retention of thin gold plating 6, 19 -- In-Reply-To: {200812091812.mB9ICdNl001716-at-ns.microscopy.com} 6, 19 -- Message-ID: {Pine.BSF.4.64.0812091754430.35130-at-monet.bestweb.net} 6, 19 -- References: {200812091812.mB9ICdNl001716-at-ns.microscopy.com} 6, 19 -- MIME-Version: 1.0 6, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
I spent many years in PCB and connector industries with micro-xrf instruments. Cross sectioning connects and PCB fingers for Au/Ni/Cu was very common. To avoid Au smear, the samples were routinely over-plated with Ni then polished. This was the best way to get reasonable cross section values. You might also consider an XRF instrument, too.
Regards,
Don Kloos VP Sales, Marketing, Business Development
Sent: Tuesday, December 09, 2008 10:17 AM To: dkloos-at-parallaxray.com
Hello Everyone, I need some advise on polishing cross-sections of electrical contacts. I'm looking at a PCB with a copper contact with a plating of nickel followed by a thin plating of gold. The gold plating reminds me of those watches you saw on TV years ago proclaiming "Generously plated with 1 micron of 24 carat gold... "
I'm carbon coating my samples and examining with the SEM
I've mounting the circuit board in a edge retention thermoplastic, but I still think I am smearing out my gold layer. Any ideas on retaining a thin layer of gold while grinding and polishing to 1um would be welcome.
stay safe........ Frank Karl.......
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 8, 21 -- From frank_karl-at-lincolnelectric.com Tue Dec 9 12:09:22 2008 8, 21 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9I9MZk028821 8, 21 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 12:09:22 -0600 8, 21 -- Subject: edge retention of thin gold plating 8, 21 -- To: Microscopy-at-microscopy.com 8, 21 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 8, 21 -- Message-ID: {OF5CF7D493.8FA73670-ON8525751A.0062DD0A-8525751A.0063B09A-at-lincolnelectric.c om} 8, 21 -- Date: Tue, 9 Dec 2008 13:08:56 -0500 8, 21 -- From: Frank_Karl-at-lincolnelectric.com 8, 21 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, 8, 21 -- CD-MIME complete at 12/09/2008 01:08:53 PM, 8, 21 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, 8, 21 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 8, 21 -- 07, 2008) at 12/09/2008 01:08:53 PM, 8, 21 -- Serialize complete at 12/09/2008 01:08:53 PM 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain; 8, 21 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From dkloos-at-parallaxray.com Tue Dec 9 13:57:49 2008 19, 28 -- Received: from cp18.heritagewebdesign.com (cp18.heritagewebdesign.com [209.90.77.54]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB9Jvn3W026565 19, 28 -- for {microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 13:57:49 -0600 19, 28 -- Received: from user-0c8gg59.cable.mindspring.com ([24.136.64.169] helo=donl) 19, 28 -- by cp18.heritagewebdesign.com with esmtpa (Exim 4.69 (FreeBSD)) 19, 28 -- (envelope-from {dkloos-at-parallaxray.com} ) 19, 28 -- id 1LA8iK-0004BC-Vl; Tue, 09 Dec 2008 12:57:49 -0700 19, 28 -- From: "Don Kloos" {dkloos-at-parallaxray.com} 19, 28 -- To: {Frank_Karl-at-lincolnelectric.com} 19, 28 -- Cc: {microscopy-at-microscopy.com} 19, 28 -- References: {200812091817.mB9IH2ap009785-at-ns.microscopy.com} 19, 28 -- Subject: RE: [Microscopy] edge retention of thin gold plating 19, 28 -- Date: Tue, 9 Dec 2008 11:57:41 -0800 19, 28 -- Message-ID: {2265E5677A7F4DCF9149C4D069A1A629-at-donl} 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="us-ascii" 19, 28 -- Content-Transfer-Encoding: 7bit 19, 28 -- X-Mailer: Microsoft Office Outlook 11 19, 28 -- Thread-Index: AclaKlboa7ApNLTGRLacn2IQIRNkAgADc9jw 19, 28 -- In-Reply-To: {200812091817.mB9IH2ap009785-at-ns.microscopy.com} 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 19, 28 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 19, 28 -- X-AntiAbuse: Primary Hostname - cp18.heritagewebdesign.com 19, 28 -- X-AntiAbuse: Original Domain - microscopy.com 19, 28 -- X-AntiAbuse: Originator/Caller UID/GID - [26 6] / [26 6] 19, 28 -- X-AntiAbuse: Sender Address Domain - parallaxray.com ==============================End of - Headers==============================
In years past we successfully did this for mil-spec electrical connectors by overplating with hard, electroless nickel. Buehler sold an electroless nickel plating solution for this purpose that worked smoothly for us but one could formulate one's own version. In deciding to adopt this sample prep we compared the plating thickness results obtained with, and without the overplating. Even though we were giving a light etch following the polishing step to bring out the grain structure (and thus removing any surface smearing) without the overplating we were seeing as much as 30% higher thickness, apparently due to compression of the malleable gold during the later stages of preparation.
John Twilley
-----Original Message----- } From: Frank_Karl-at-lincolnelectric.com } Sent: Dec 9, 2008 1:09 PM } To: jtwilley-at-sprynet.com } Subject: [Microscopy] edge retention of thin gold plating } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 5, 26 -- From jtwilley-at-sprynet.com Tue Dec 9 21:28:23 2008 5, 26 -- Received: from elasmtp-curtail.atl.sa.earthlink.net (elasmtp-curtail.atl.sa.earthlink.net [209.86.89.64]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBA3SMil018737 5, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Dec 2008 21:28:23 -0600 5, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 26 -- s=dk20050327; d=sprynet.com; 5, 26 -- b=nYvDtY7gtkHsOYMHpq8lPApaE5NNT+TOupQlEIWTMpFAtGGlgbxC7XY+FhLwWE8L; 5, 26 -- h=Message-ID:Date:From:Reply-To:To:Subject:Cc:Mime-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:X-ELNK-Trace:X-Originating-IP; 5, 26 -- Received: from [209.86.224.51] (helo=mswamui-thinleaf.atl.sa.earthlink.net) 5, 26 -- by elasmtp-curtail.atl.sa.earthlink.net with esmtpa (Exim 4.67) 5, 26 -- (envelope-from {jtwilley-at-sprynet.com} ) 5, 26 -- id 1LAFjs-0002ke-Et; Tue, 09 Dec 2008 22:27:52 -0500 5, 26 -- Received: from 70.23.77.176 by webmail.atl.earthlink.net with HTTP; Tue, 9 Dec 2008 22:27:27 -0500 5, 26 -- Message-ID: {14599384.1228879647320.JavaMail.root-at-mswamui-thinleaf.atl.sa.earthlink.net} 5, 26 -- Date: Tue, 9 Dec 2008 22:27:27 -0500 (GMT-05:00) 5, 26 -- From: jtwilley-at-sprynet.com 5, 26 -- Reply-To: jtwilley-at-sprynet.com 5, 26 -- To: Frank_Karl-at-lincolnelectric.com 5, 26 -- Subject: Re: [Microscopy] edge retention of thin gold plating 5, 26 -- Cc: Microscopy-at-microscopy.com 5, 26 -- Mime-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=UTF-8 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- X-Mailer: EarthLink Zoo Mail 1.0 5, 26 -- X-ELNK-Trace: adbb89a220ca650b5741bb2dafe8270558bc2ddbf51b90e4cc696769d268eef5ea108c17f89ea767350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 5, 26 -- X-Originating-IP: 209.86.224.51 ==============================End of - Headers==============================
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Question: We have some particular questions regarding using a JEOL TEM 2010F with an Analytical pole piece for getting lattice images. I appreciate if any of you having such instrument setting could contact me offline. Thank you much. Amelia
L. Amelia Dempere E-mail: ademp-at-mse.ufl.edu Phone 352-392-6985 Major Analytical Instrumentation Center University of Florida
My address has been successfully recorded, and I am interested in "TEM - Dislocation Loop Analysis and so forth". Thank you all. Daniela.
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==============================Original Headers============================== 6, 36 -- From daniela.nunes-at-ist.utl.pt Wed Dec 10 02:29:28 2008 6, 36 -- Received: from smtp1.ist.utl.pt (smtp1.ist.utl.pt [193.136.128.21]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBA8TR6g018778; 6, 36 -- Wed, 10 Dec 2008 02:29:27 -0600 6, 36 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 6, 36 -- by smtp1.ist.utl.pt (Postfix) with ESMTP id E801C7000046; 6, 36 -- Wed, 10 Dec 2008 08:29:25 +0000 (WET) 6, 36 -- X-Virus-Scanned: by amavisd-new-2.5.3 (20071212) (Debian) at ist.utl.pt 6, 36 -- Received: from smtp1.ist.utl.pt ([127.0.0.1]) 6, 36 -- by localhost (smtp1.ist.utl.pt [127.0.0.1]) (amavisd-new, port 10025) 6, 36 -- with LMTP id 02dtn9WLJc3p; Wed, 10 Dec 2008 08:29:20 +0000 (WET) 6, 36 -- Received: from mail1.ist.utl.pt (mail1.ist.utl.pt [193.136.128.10]) 6, 36 -- by smtp1.ist.utl.pt (Postfix) with ESMTP id E0C4A700043E; 6, 36 -- Wed, 10 Dec 2008 08:29:20 +0000 (WET) 6, 36 -- Received: by mail1.ist.utl.pt (Postfix, from userid 33) 6, 36 -- id 9076C1400532; Wed, 10 Dec 2008 08:29:20 +0000 (WET) 6, 36 -- Received: from 193.137.43.138 (193.137.43.138 [193.137.43.138]) by 6, 36 -- mail.ist.utl.pt (Horde MIME library) with HTTP; Wed, 10 Dec 2008 08:29:20 6, 36 -- +0000 6, 36 -- Message-ID: {20081210082920.vvnfgkva8gkks8kc-at-mail1.ist.utl.pt} 6, 36 -- Date: Wed, 10 Dec 2008 08:29:20 +0000 6, 36 -- From: DANIELA DA SILVA NUNES {daniela.nunes-at-ist.utl.pt} 6, 36 -- To: ListServer-at-microscopy.com 6, 36 -- Cc: microscopy-at-microscopy.com 6, 36 -- Subject: Re: Welcome to the Microscopy ListServer:Info,Rules & FAQ 6, 36 -- References: {200812100636.mBA6a38G003025-at-ns.microscopy.com} 6, 36 -- In-Reply-To: {200812100636.mBA6a38G003025-at-ns.microscopy.com} 6, 36 -- MIME-Version: 1.0 6, 36 -- Content-Type: text/plain; 6, 36 -- charset=ISO-8859-1; 6, 36 -- DelSp="Yes"; 6, 36 -- format="flowed" 6, 36 -- Content-Disposition: inline 6, 36 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.3) 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBA8TR6g018778 ==============================End of - Headers==============================
} Is a small bottle of chloroform really something so deadly as to make } one go so much out of one's way to avoid? I do not think so. ... } Let's just use common sense and not let safety officers make us } paranoid. } } Vlad
I agree.
Let's see MSDS: ---------------------------------------------------------------------- Warning! May cause severe eye irritation and possible injury. Flammable liquid and vapor. May be absorbed through intact skin. May cause skin and respiratory tract irritation. May cause central nervous system depression. May cause liver damage. May cause kidney damage. May cause adverse reproductive effects. Target Organs: Kidneys, central nervous system, liver.
Potential Health Effects Eye: Contact with eyes may cause severe irritation, and possible eye burns. Skin: May cause skin irritation. Prolonged and/or repeated contact may cause defatting of the skin and dermatitis. May be absorbed through the skin. Ingestion: Cannot be made non-poisonous. Causes gastrointestinal irritation with nausea, vomiting and diarrhea. May cause kidney damage. May cause liver damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. May cause respiratory tract irritation. Prolonged exposure may result in dizziness and general weakness. Chronic: Prolonged or repeated eye contact may cause conjunctivitis. Prolonged or repeated exposure may cause adverse reproductive effects. May cause liver and kidney damage.
Section 4 - First Aid Measures Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. Skin: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Remove contaminated clothing and shoes. ... ------------------------------------------------------------------------ --------
Very interesting reading, isn't it? But it is not about chloroform, it is about very pure Ethyl Alcohol-D, 99.5+ Atom % D.
In this MSDS I like especially part about removing clothes in case of contamination. Shouldn't it be posted in all bars? Then, if you splash whiskey on your trousers you should remove them immediately, otherwise you will be transported to physician at your own expense.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Not to throw a whole other monkey wrench into the mix of heat pen vs solvent...
For Spurr's and Moll/Epons, I've only ever used Xylenes to relax the sections. I still have the "wand" I made as an undergraduate, and still use it. A small corner of filter paper attached to the end of a wood applicator stick. I mostly use Spurr's and find the Xylenes work great. That or I just walk away (or get up to answer questions or help someone else) and they are nice and relaxed when I come back to sit down again.
The heat pen mentioned earlier on the list is a jeweler's model, and I always had great success stretching Spurr's sections with it. It does take a touch more time than the Xylenes and it does work better on thinner rather than thicker sections.
Are Xylenes better or worse for you than Chloroform? Honestly off the top of my head they are both in the nasty chemical category and I don't have a least favorite, just habit I guess. I will likely be moving the lab over to heat pens soon, but I will not prevent users of our facility from using their method of choice to relax sections.
I just wanted to chime in on a chloroform alternative, (not to prolong the discussion).
Geoff Williams Leduc Bioimaging Facility Manager Brown University
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Wednesday, December 10, 2008 1:49 PM To: Williams, Geoffrey
} Is a small bottle of chloroform really something so deadly as to make } one go so much out of one's way to avoid? I do not think so. ... } Let's just use common sense and not let safety officers make us } paranoid. } } Vlad
I agree.
Let's see MSDS: ---------------------------------------------------------------------- Warning! May cause severe eye irritation and possible injury. Flammable liquid and vapor. May be absorbed through intact skin. May cause skin and respiratory tract irritation. May cause central nervous system depression. May cause liver damage. May cause kidney damage. May cause adverse reproductive effects. Target Organs: Kidneys, central nervous system, liver.
Potential Health Effects Eye: Contact with eyes may cause severe irritation, and possible eye burns. Skin: May cause skin irritation. Prolonged and/or repeated contact may cause defatting of the skin and dermatitis. May be absorbed through the skin. Ingestion: Cannot be made non-poisonous. Causes gastrointestinal irritation with nausea, vomiting and diarrhea. May cause kidney damage. May cause liver damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. May cause respiratory tract irritation. Prolonged exposure may result in dizziness and general weakness. Chronic: Prolonged or repeated eye contact may cause conjunctivitis. Prolonged or repeated exposure may cause adverse reproductive effects. May cause liver and kidney damage.
Section 4 - First Aid Measures Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. Skin: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Remove contaminated clothing and shoes. ... ------------------------------------------------------------------------ --------
Very interesting reading, isn't it? But it is not about chloroform, it is about very pure Ethyl Alcohol-D, 99.5+ Atom % D.
In this MSDS I like especially part about removing clothes in case of contamination. Shouldn't it be posted in all bars? Then, if you splash whiskey on your trousers you should remove them immediately, otherwise you will be transported to physician at your own expense.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
We have old SEM Philips 525 and TEM JEOL JEM 2000FX which we would like to get rid of. The only my request is that I don't want to be involved in shipping. Someone who offers a fee will definitely have a priority. If you have any interest, please contact me directly.
**************************************************************************************** Dr. Sergey Prikhodko Department of Materials Science and Engineering 2264-D Boelter Hall University of California Los Angeles, CA 90095-1595
Tel: (310) 825-9735 Fax: (310) 206-7353
==============================Original Headers============================== 5, 23 -- From sergey-at-seas.ucla.edu Wed Dec 10 14:51:31 2008 5, 23 -- Received: from smtp-b.seas.ucla.edu (smtp-b.seas.ucla.edu [164.67.100.46]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBAKpURq017675 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Dec 2008 14:51:30 -0600 5, 23 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 5, 23 -- by smtp-b.seas.ucla.edu (8.14.3/8.14.3/MTA-TX) with ESMTP id mBAKpTn8018206 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Dec 2008 12:51:29 -0800 5, 23 -- X-Virus-Scanned: amavisd-new at seas.ucla.edu 5, 23 -- Received: from smtp-b.seas.ucla.edu ([127.0.0.1]) 5, 23 -- by localhost (smtp-b.seas.ucla.edu [127.0.0.1]) (amavisd-new, port 10024) 5, 23 -- with LMTP id xf94xnBX5ljY for {Microscopy-at-microscopy.com} ; 5, 23 -- Wed, 10 Dec 2008 12:51:29 -0800 (PST) 5, 23 -- Received: from Beam.seas.ucla.edu (beam.seas.ucla.edu [128.97.83.27]) 5, 23 -- by smtp-b.seas.ucla.edu (8.14.3/8.14.3/MTA-RX) with ESMTP id mBAKpOUG018199 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Dec 2008 12:51:29 -0800 5, 23 -- Message-Id: {6.2.0.14.2.20081210125053.02341508-at-pop.seas.ucla.edu} 5, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.0.14 5, 23 -- Date: Wed, 10 Dec 2008 12:51:44 -0800 5, 23 -- To: Microscopy-at-microscopy.com 5, 23 -- From: Sergey Prikhodko {sergey-at-seas.ucla.edu} 5, 23 -- Subject: old SEM Philips 525 and TEM JEOL JEM 2000FX 5, 23 -- Mime-Version: 1.0 5, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both swatkins-at-pitt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: swatkins-at-pitt.edu Name: simon watkins
Organization: University of Pittsburgh
Title-Subject: [Filtered] QFM 2k09
Question: Folks, we would like to announce that enrollment for Quantitative Fluorescence Microscopy 2k09 at the Mount Desert Island Marine Laboratory in Maine is now open. The tremendous success of the course over the last several years has encouraged us to run it again next year, and as it is December it is time to start our enrollment. This is an intensive lab/lecture course, focusing on fluorescence microscopy in all its forms, including widefield, Confocal, Multiphoton, Decon, TIRF, FRET etc, though with an emphasis on live cell methods. We also encourage students to provide their own specimens such that the hands on components of the course are as enriching as possible (in fact last year at one point, the technical help were growing 64 different cell lines for our students). Enrollment is highly competitive, so if you think that you or your colleagues would benefit from the course please enroll as soon as possible.
As the course is moving into its second decade, we have decided to make a couple of significant changes to the enrollment process:
1: Perhaps the most important is that Alumni are eligible to return. Because we made sweeping changes in the curriculum and faculty last year, and all aspects of imaging have advanced enormously since we started the course we feel it is time to allow past students to take the course again. So... alumni... you are welcome to return to the course. Please point out that you are an alumnus in the application (it won't affect eligibility).
2: The second big change which has been driven by a common student request that we have rolling admissions. We have decided to act on this. Our first student group will be accepted late this month, then February and the final group in April. There are no particular quotas for each block, rather students should have good projects that suit the flavor and goals of the course. The dates for course are from Saturday May 30th 2009 to the following Friday (June 5th) . The url for the course is http://www.cbi.pitt.edu/qfm/index.html
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both samantha_angel_murray-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] EM - photoconversion of DAB by DiI for EM
Question: I was tracing cell dynamics fluorescently with a Molecular Probes carbocyanine dye DiI. Iím afraid I didnít do my homework properly and thought DiI precipitated to electron dense particles without first processing. I have since read that the ppt is achieved by photoconversion of DAB by DiI. Unfortunately, the samples have since been embedded in Spurr Resin, my question therefore, is there anything I can do to get through the Resin to process the sample with DAB for TEM?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both shum-at-fas.harvard.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: shum-at-fas.harvard.edu Name: Anderson Shum
Title-Subject: [Filtered] Cryo-SEM in New England?
Question: I'm wondering if anybody knows of a cryo-sem system in New England region of the US. I would like to use it for imaging some emulsion systems.
I was just reading a paper from 1966 and the author used ice-cold uranyl acetate for contrasting. Then I thought: "well why not?" Actually lower temperatures should limit the precipitation. This is no big deal with UAc but with lead citrate may offer a significant improvement. Anyone ever tried? Any reason not to do it? Does it make sense? ...
Stephane
==============================Original Headers============================== 6, 21 -- From nizets2-at-yahoo.com Thu Dec 11 03:02:35 2008 6, 21 -- Received: from web110805.mail.gq1.yahoo.com (web110805.mail.gq1.yahoo.com [67.195.13.228]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBB92Zld022365 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 03:02:35 -0600 6, 21 -- Received: (qmail 97460 invoked by uid 60001); 11 Dec 2008 09:02:34 -0000 6, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 21 -- s=s1024; d=yahoo.com; 6, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 21 -- b=auZbrD8qwJ2P+sRd055b5BR2uY4GqGXA1q62xjVZUcn6jKQzionyKliP8yz9yXONTWaN0UO42thPKKmkuQtoQF6k5nHrSSJoey2ZOpDTZ4FKDfsX3Ioi60vsKjtiYU8j8kp2AnsbK+7enu40eiBTrtzfGvwlXnsW6yDOTAXQYF8=; 6, 21 -- X-YMail-OSG: ka7xjUAVM1kZfxiOFx6bYZ_vFUXRPqgDEjuaaK1Q_LppHyBG8ExLDt99k0fd5zVlUtMBOx.EgFQQXb1mKnqwUdD4DijtQ40fe9NYrtnRuPMXKT0xPUqN2x.gV4FVKV8_7IaIW1QozYIKYNJMfAcu_dliEuj1_LS8weSoPNxyD0r7jN2Z6KALanF01EOz_w-- 6, 21 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 11 Dec 2008 01:02:34 PST 6, 21 -- X-Mailer: YahooMailRC/1155.32 YahooMailWebService/0.7.260.1 6, 21 -- Date: Thu, 11 Dec 2008 01:02:34 -0800 (PST) 6, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 21 -- Subject: cold constrasting ? 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; charset=iso-8859-1 6, 21 -- Message-ID: {730741.97446.qm-at-web110805.mail.gq1.yahoo.com} 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBB92Zld022365 ==============================End of - Headers==============================
(1)Cave: dissociation of "salts" lowered (concentration?)==} automatic } Ultrostainers { use "warm solutions" or at least provide staining at elevated temperatures....why this is provided??
(2)Cave: condensation of water vapour from room air: how would you like to handle that? Especially not favoured for Uranylacetate (but there are few exceptions, remembering "old-fashioned" [neuro-]secretory granule staining or special stain(s) where "traces" of water in absolute methanolic/ethanolic uranylacetate are not only allowed but necessary / desirable to get the right staining effect).
(3)Cave: binding properties of staining fluid in the cold perhaps are decreased (see 1) )
(4) most recipes for lead citrate require } heating { or at least "hot" water for dissolution of powders and e. g. conversion from Pb[No3]2 to Pb-citrate (REYNOLDS 1963 as well as VENABLE&COGGESHALL 1965).
EM-staining at room temperature (if ou do it manually) more/less is quite comfortable ("as we have always done"), Perhaps if you have an } ultrostainer { you could try "cold staining"
Best
Wolfgang Salzburg-Austria
} -----Ursprüngliche Nachricht----- } Von: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Gesendet: Donnerstag, 11. Dezember 2008 10:06 } An: Wolfgang Muss } Betreff: [Microscopy] cold constrasting ? } } } } } } -------------------------------------------------------------- } -------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------- } -------------- } } Dear all, } } I was just reading a paper from 1966 and the author used } ice-cold uranyl acetate for contrasting. } Then I thought: "well why not?" } Actually lower temperatures should limit the precipitation. } This is no big deal with UAc but with lead citrate may offer } a significant improvement. } Anyone ever tried? Any reason not to do it? Does it make sense? ... } } Stephane } } } } } } ==============================Original } Headers============================== } 6, 21 -- From nizets2-at-yahoo.com Thu Dec 11 03:02:35 2008 } 6, 21 -- Received: from web110805.mail.gq1.yahoo.com } (web110805.mail.gq1.yahoo.com [67.195.13.228]) } 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with SMTP id mBB92Zld022365 } 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec } 2008 03:02:35 -0600 } 6, 21 -- Received: (qmail 97460 invoked by uid 60001); 11 Dec } 2008 09:02:34 -0000 } 6, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 21 -- s=s1024; d=yahoo.com; } 6, 21 -- } h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Vers ion:Content-Type:Content-Transfer-} Encoding:Message-ID; } 6, 21 -- } b=auZbrD8qwJ2P+sRd055b5BR2uY4GqGXA1q62xjVZUcn6jKQzionyKliP8yz9 } yXONTWaN0UO42thPKKmkuQtoQF6k5nHrSSJoey2ZOpDTZ4FKDfsX3Ioi60vsKj } tiYU8j8kp2AnsbK+7enu40eiBTrtzfGvwlXnsW6yDOTAXQYF8=; } 6, 21 -- X-YMail-OSG: } ka7xjUAVM1kZfxiOFx6bYZ_vFUXRPqgDEjuaaK1Q_LppHyBG8ExLDt99k0fd5z } VlUtMBOx.EgFQQXb1mKnqwUdD4DijtQ40fe9NYrtnRuPMXKT0xPUqN2x.gV4FV } KV8_7IaIW1QozYIKYNJMfAcu_dliEuj1_LS8weSoPNxyD0r7jN2Z6KALanF01EOz_w-- } 6, 21 -- Received: from [80.122.101.100] by } web110805.mail.gq1.yahoo.com via HTTP; Thu, 11 Dec 2008 01:02:34 PST } 6, 21 -- X-Mailer: YahooMailRC/1155.32 YahooMailWebService/0.7.260.1 } 6, 21 -- Date: Thu, 11 Dec 2008 01:02:34 -0800 (PST) } 6, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 21 -- Subject: cold constrasting ? } 6, 21 -- To: microscopy-at-microscopy.com } 6, 21 -- MIME-Version: 1.0 } 6, 21 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 21 -- Message-ID: {730741.97446.qm-at-web110805.mail.gq1.yahoo.com} } 6, 21 -- Content-Transfer-Encoding: 8bit } 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit } by ns.microscopy.com id mBB92Zld022365 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 12, 36 -- From W.Muss-at-salk.at Thu Dec 11 04:18:29 2008 12, 36 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 12, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBAISID015181 12, 36 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 04:18:28 -0600 12, 36 -- Received: from localhost (localhost [127.0.0.1]) 12, 36 -- by hermes.salk.at (Postfix) with ESMTP id D9CA5C3864 12, 36 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 11:18:26 +0100 (CET) 12, 36 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 12, 36 -- Received: from hermes.salk.at ([127.0.0.1]) 12, 36 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 12, 36 -- with ESMTP id w4NAdVT8-nc0 for {microscopy-at-microscopy.com} ; 12, 36 -- Thu, 11 Dec 2008 11:18:26 +0100 (CET) 12, 36 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 12, 36 -- by hermes.salk.at (Postfix) with ESMTP id 70CB4C3863 12, 36 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 11:18:26 +0100 (CET) 12, 36 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.132]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.3959); 12, 36 -- Thu, 11 Dec 2008 11:18:26 +0100 12, 36 -- Content-class: urn:content-classes:message 12, 36 -- MIME-Version: 1.0 12, 36 -- Content-Type: text/plain; 12, 36 -- charset="iso-8859-1" 12, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 36 -- Subject: [Microscopy] Re: cold constrasting ? 12, 36 -- Date: Thu, 11 Dec 2008 11:18:26 +0100 12, 36 -- Message-ID: {06B4ED29F824524E98E8AA5BB64070625D0714-at-N1RZ116.lksdom21.lks.local} 12, 36 -- In-Reply-To: {200812110905.mBB95fjY027944-at-ns.microscopy.com} 12, 36 -- X-MS-Has-Attach: 12, 36 -- X-MS-TNEF-Correlator: 12, 36 -- Thread-Topic: [Microscopy] Re: cold constrasting ? 12, 36 -- Thread-Index: Aclbb6cTvxGonI1BS76ogI0m/pkAfwAB9JUg 12, 36 -- From: "Wolfgang Muss" {W.Muss-at-salk.at} 12, 36 -- To: {microscopy-at-microscopy.com} 12, 36 -- X-OriginalArrivalTime: 11 Dec 2008 10:18:26.0439 (UTC) FILETIME=[CE23C170:01C95B79] 12, 36 -- X-Scanned-By: SALK-Content-Filter 12, 36 -- Content-Transfer-Encoding: 8bit 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBAISID015181 ==============================End of - Headers==============================
I'd be interested in any opinions/suggestions for video recording using current TEM-CCD cameras.
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 2, 34 -- From marksmsa-at-gmail.com Thu Dec 11 08:32:43 2008 2, 34 -- Received: from wa-out-1112.google.com (wa-out-1112.google.com [209.85.146.176]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBEWhi7015013 2, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 08:32:43 -0600 2, 34 -- Received: by wa-out-1112.google.com with SMTP id v33so470498wah.2 2, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 06:32:42 -0800 (PST) 2, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 34 -- d=gmail.com; s=gamma; 2, 34 -- h=domainkey-signature:received:received:message-id:date:from:to 2, 34 -- :subject:mime-version:content-type:content-transfer-encoding 2, 34 -- :content-disposition; 2, 34 -- bh=vi3SwN0PBedTHk25AThAVd1zExkxIZTOwBPY5QzD0to=; 2, 34 -- b=pdnKppIgZAx8SiGku7IOnwYfwJPPu6IYvkH+sSsAFGdJSmE/5PCFdRbRnLKYE2wf7O 2, 34 -- Wfx/3TD87kza8i8tIijkUZTw5ci097kqclQlNFSnQxRuOxVD+umAO8PB8vyKikP5C0kM 2, 34 -- h0eL5k979Sw0FHMmyWhZkAMHLbhE2Y9auF1sQ= 2, 34 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 34 -- d=gmail.com; s=gamma; 2, 34 -- h=message-id:date:from:to:subject:mime-version:content-type 2, 34 -- :content-transfer-encoding:content-disposition; 2, 34 -- b=O7PyN71zHfFl1W9H6K5YhcfxdetzXfvE+wzUYXa93Xw3DOvdFYHV4EwVWj32QrH67l 2, 34 -- IPLZMfZPzYuGIPKGzlHhWr6yjMVmJJq8gCj/U7Eu4GmR0zn4LGDbCKmPzZKCpHtxDxHy 2, 34 -- I2M8xfOBzapo5A8qBCQzt8yYnQhiixTqIkIHM= 2, 34 -- Received: by 10.114.196.1 with SMTP id t1mr1892757waf.107.1229005962266; 2, 34 -- Thu, 11 Dec 2008 06:32:42 -0800 (PST) 2, 34 -- Received: by 10.114.88.5 with HTTP; Thu, 11 Dec 2008 06:32:41 -0800 (PST) 2, 34 -- Message-ID: {e13ba6260812110632q42589b36xfc682c99cd52eccf-at-mail.gmail.com} 2, 34 -- Date: Thu, 11 Dec 2008 08:32:41 -0600 2, 34 -- From: "L Marks" {marksmsa-at-gmail.com} 2, 34 -- To: microscopy {Microscopy-at-microscopy.com} 2, 34 -- Subject: Digital TV recording 2, 34 -- MIME-Version: 1.0 2, 34 -- Content-Type: text/plain; charset=ISO-8859-1 2, 34 -- Content-Transfer-Encoding: 7bit 2, 34 -- Content-Disposition: inline ==============================End of - Headers==============================
When we do EDS on biological samples we use a standard TEM specimen with a drop of sample on a carbon film. This is often helpful if the SEM has a STEM detector for imaging. When trying to detect or particularly "map" the elemental concentrations, the issue is one of signal to noise and possibly very low concentrations in the biological sample.
Roseann
Roseann Csencsits, PhD Scientist in Charge - Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Oct 8, 2008, at 3:39 PM, grace.jang-at-asu.edu wrote:
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==============================Original Headers============================== 13, 26 -- From RCsencsits-at-lbl.gov Thu Dec 11 10:37:09 2008 13, 26 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBGb9KJ031816 13, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 10:37:09 -0600 13, 26 -- X-Ironport-SBRS: 2.3 13, 26 -- X-IronPort-Anti-Spam-Filtered: true 13, 26 -- X-IronPort-Anti-Spam-Result: Ap8CAFHQQEmAAykMe2dsb2JhbACTTQEBFiIFrWkBAQeLeIFzAQGBBINz 13, 26 -- X-IronPort-AV: E=Sophos;i="4.33,753,1220252400"; 13, 26 -- d="scan'208";a="8402851" 13, 26 -- Received: from mta2.lbl.gov ([128.3.41.12]) 13, 26 -- by ironport4.lbl.gov with ESMTP; 11 Dec 2008 08:37:07 -0800 13, 26 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) 13, 26 -- by mta2.lbl.gov (8.13.8/8.13.8) with ESMTP id mBBGb6sF017756; 13, 26 -- Thu, 11 Dec 2008 08:37:07 -0800 (PST) 13, 26 -- Cc: Microscopy-at-microscopy.com 13, 26 -- Message-Id: {86CF042A-5DF5-4DFD-8D8D-B57F12C61470-at-lbl.gov} 13, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 13, 26 -- To: grace.jang-at-asu.edu 13, 26 -- In-Reply-To: {200810082239.m98MdZTj009161-at-ns.microscopy.com} 13, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 13, 26 -- Content-Transfer-Encoding: 7bit 13, 26 -- Mime-Version: 1.0 (Apple Message framework v929.2) 13, 26 -- Subject: Re: [Microscopy] viaWWW: element mapping on E.coli by EDX 13, 26 -- Date: Thu, 11 Dec 2008 07:37:52 -0800 13, 26 -- References: {200810082239.m98MdZTj009161-at-ns.microscopy.com} 13, 26 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
The most rigorous way - and most likely the only credible way - to do this is to use cryo techniques. There are multiple books and papers on this topic going back at least 30 years. Try Googling!
Best of luck,
Peter I
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Dear Samantha, DiI is not fixable by aldehyde fixation, it diffuses into the lipid layer of the plasma membrane. Since epoxy embedding requires dehydration by ethanol and the use of a transition solvent such as propylene oxide or acetone, the lipids and most of the DiI have been stripped from the tissue. The photoconversion must be performed prior to embedding. Maybe you could use a fixable tracer conjugated to a fluorescent dye that also has biotin group for labeling with an avidin-peroxidase method or some similar type of approach
Good Luck, glen . Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
****************************************************************************** The box said "Requires WindowsXP or better", so I bought a Macintosh. ******************************************************************************
On Dec 10, 2008, at 11:38 PM, samantha_angel_murray-at-hotmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both } samantha_angel_murray-at-hotmail.com as well as } the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: samantha_angel_murray-at-hotmail.com } Name: Samantha Murray } } Organization: Portsmouth University, UK } } Title-Subject: [Filtered] EM - photoconversion of DAB by DiI for EM } } Question: I was tracing cell dynamics } fluorescently with a Molecular Probes } carbocyanine dye DiI. Iím afraid I didnít do my } homework properly and thought DiI precipitated to } electron dense particles without first } processing. I have since read that the ppt is } achieved by photoconversion of DAB by DiI. } Unfortunately, the samples have since been } embedded in Spurr Resin, my question therefore, } is there anything I can do to get through the } Resin to process the sample with DAB for TEM? } } Many thanks } } } Login Host: 78.151.149.130 } --------------------------------------------------------------------------- } } } ==============================Original } Headers============================== } 9, 13 -- From zaluzec-at-microscopy.com Thu Dec 11 01:36:11 2008 } 9, 13 -- Received: from [10.158.144.177] (msdvpn8.msd.anl.gov } [130.202.238.72]) } 9, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mBB7a9DW012964 } 9, 13 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 01:36:11 } -0600 } 9, 13 -- Mime-Version: 1.0 } 9, 13 -- Message-Id: {p06240805c5667359ee0c-at-[10.158.144.177]} } 9, 13 -- Date: Thu, 11 Dec 2008 08:36:09 +0100 } 9, 13 -- To: microscopy-at-microscopy.com } 9, 13 -- From: samantha_angel_murray-at-hotmail.com (by way of } MicroscopyListserver) } 9, 13 -- Subject: viaWWW: EM - photoconversion of DAB by DiI for EM } 9, 13 -- Content-Type: text/plain; charset="iso-8859-1" ; } format="flowed" } 9, 13 -- Content-Transfer-Encoding: 8bit } 9, 13 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mBB7a9DW012964 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 28 -- From glenmac-at-u.washington.edu Thu Dec 11 11:20:51 2008 8, 28 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBHKpuv032355 8, 28 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 11:20:51 -0600 8, 28 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 8, 28 -- by mxout7.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id mBBHKoqf012295 8, 28 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK); 8, 28 -- Thu, 11 Dec 2008 09:20:50 -0800 8, 28 -- X-Auth-Received: from D-128-95-178-137.dhcp4.washington.edu (D-128-95-178-137.dhcp4.washington.edu [128.95.178.137]) 8, 28 -- (authenticated authid=glenmac) 8, 28 -- by smtp.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id mBBHKnsT009236 8, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT); 8, 28 -- Thu, 11 Dec 2008 09:20:50 -0800 8, 28 -- Cc: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 8, 28 -- Message-Id: {940DB9CC-8707-4077-83DD-DBE26903C096-at-u.washington.edu} 8, 28 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 8, 28 -- To: samantha_angel_murray-at-hotmail.com 8, 28 -- In-Reply-To: {200812110738.mBB7cDag018784-at-ns.microscopy.com} 8, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 8, 28 -- Mime-Version: 1.0 (Apple Message framework v929.2) 8, 28 -- Subject: Re: [Microscopy] viaWWW: EM - photoconversion of DAB by DiI for EM 8, 28 -- Date: Thu, 11 Dec 2008 09:20:49 -0800 8, 28 -- References: {200812110738.mBB7cDag018784-at-ns.microscopy.com} 8, 28 -- X-Mailer: Apple Mail (2.929.2) 8, 28 -- X-PMX-Version: 5.5.0.356843, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.12.11.171328 8, 28 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=8%, Report='BODY_SIZE_4000_4999 0, BODY_SIZE_5000_LESS 0, __BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_419_BODY_WEBMAIL 0, __FRAUD_419_CONTACT_NAME 0, __FRAUD_419_WEBMAIL 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0' 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBHKpuv032355 ==============================End of - Headers==============================
O.k. just found 20 sealed ampoules of 10ml 50% EM Grade glutaraldehyde. Stored in a refrigerator, purchase from one of the EM supply companies (so not some unknown quality vendor).
BUT they were purchased in 1994. Yes, we could yes go ahead and prep some samples, but rather than waste the time and effort I thought I'd get the feeling from all you folks: (1) Garbage?, (2) Likely to be good?, (3) I don't know have to test?
Thanks!
(I know it reminds me of that crystal OsO4 find a little while ago). Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 6, 22 -- From edelmare-at-muohio.edu Thu Dec 11 13:23:56 2008 6, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.69]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJNuXH021007 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 13:23:56 -0600 6, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBJO375022156 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:24:03 -0500 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 6, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBJNtxf014380 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:23:55 -0500 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 6, 22 -- To: microscopy-at-Microscopy.com 6, 22 -- Date: Thu, 11 Dec 2008 14:23:55 -0500 6, 22 -- MIME-Version: 1.0 6, 22 -- Subject: Shelf life of Glutaraldehyde 6, 22 -- Message-ID: {4941227B.1083.6316340-at-edelmare.muohio.edu} 6, 22 -- Priority: normal 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 22 -- Content-type: text/plain; charset=US-ASCII 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Content-description: Mail message body 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69 ==============================End of - Headers==============================
I don't know the glutaraldehyde shelf life but am interested since I have some 2-3 year old ampoules myself. I am guessing it is good. I can't resist mentioning that two years ago I got a huge stock of over 20 grams of free crystalline osmium tetroxide - the only problem was that it was packaged by Merck in 1940! The ampoules were in individual wooden sleeves. I found no difference in osmication with it compared to recently bought osmium.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Thursday, December 11, 2008 1:25 PM To: Phillips, Thomas E.
O.k. just found 20 sealed ampoules of 10ml 50% EM Grade glutaraldehyde. Stored in a refrigerator, purchase from one of the EM supply companies (so not some unknown quality vendor).
BUT they were purchased in 1994. Yes, we could yes go ahead and prep some samples, but rather than waste the time and effort I thought I'd get the feeling from all you folks: (1) Garbage?, (2) Likely to be good?, (3) I don't know have to test?
Thanks!
(I know it reminds me of that crystal OsO4 find a little while ago). Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 6, 22 -- From edelmare-at-muohio.edu Thu Dec 11 13:23:56 2008 6, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.69]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJNuXH021007 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 13:23:56 -0600 6, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBJO375022156 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:24:03 -0500 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 6, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBJNtxf014380 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:23:55 -0500 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 6, 22 -- To: microscopy-at-Microscopy.com 6, 22 -- Date: Thu, 11 Dec 2008 14:23:55 -0500 6, 22 -- MIME-Version: 1.0 6, 22 -- Subject: Shelf life of Glutaraldehyde 6, 22 -- Message-ID: {4941227B.1083.6316340-at-edelmare.muohio.edu} 6, 22 -- Priority: normal 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 22 -- Content-type: text/plain; charset=US-ASCII 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Content-description: Mail message body 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From PhillipsT-at-missouri.edu Thu Dec 11 13:40:16 2008 16, 29 -- Received: from mxtip01-missouri-out.um.umsystem.edu (mxtip01-missouri-out.um.umsystem.edu [209.106.229.45]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJeFRH002456 16, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 13:40:16 -0600 16, 29 -- X-IronPort-Anti-Spam-Filtered: true 16, 29 -- X-IronPort-Anti-Spam-Result: ApoEAPT6QEnRauUp/2dsb2JhbADDCwEJhkGFMAGBcoEG 16, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 16, 29 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 11 Dec 2008 13:40:15 -0600 16, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 29 -- Thu, 11 Dec 2008 13:40:15 -0600 16, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5 16, 29 -- Content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="us-ascii" 16, 29 -- Subject: RE: [Microscopy] Shelf life of Glutaraldehyde 16, 29 -- Date: Thu, 11 Dec 2008 13:39:53 -0600 16, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD059022C7-at-UM-XMAIL06.um.umsystem.edu} 16, 29 -- In-Reply-To: {200812111925.mBBJP3qN022143-at-ns.microscopy.com} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] Shelf life of Glutaraldehyde 16, 29 -- Thread-Index: AclbxiuZ6TpJRbVRSiGzNzHumWAkfAAAaANg 16, 29 -- References: {200812111925.mBBJP3qN022143-at-ns.microscopy.com} 16, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 29 -- To: {Microscopy-at-microscopy.com} 16, 29 -- X-OriginalArrivalTime: 11 Dec 2008 19:40:15.0492 (UTC) FILETIME=[4A4D0C40:01C95BC8] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBJeFRH002456 ==============================End of - Headers==============================
Dear Richard, This nudged my memory. take a look in Hayat, Principles and Techniques, Vol. 1, pp. 78-81 You might be able to scan it on a UV spectrophotometer. Pure glut absorbs at 280 nm, other peaks would indicate impurities.,
Glen On Dec 11, 2008, at 11:41 AM, PhillipsT-at-missouri.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I don't know the glutaraldehyde shelf life but am interested since I } have some 2-3 year old ampoules myself. I am guessing it is good. I } can't resist mentioning that two years ago I got a huge stock of } over 20 } grams of free crystalline osmium tetroxide - the only problem was that } it was packaged by Merck in 1940! The ampoules were in individual } wooden } sleeves. I found no difference in osmication with it compared to } recently bought osmium. } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Chair, MU Faculty Council } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } -----Original Message----- } X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] } Sent: Thursday, December 11, 2008 1:25 PM } To: Phillips, Thomas E. } Subject: [Microscopy] Shelf life of Glutaraldehyde } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } O.k. just found 20 sealed ampoules of 10ml 50% EM Grade } glutaraldehyde. Stored in a refrigerator, purchase from one of the } EM supply companies (so not some unknown quality vendor). } } BUT they were purchased in 1994. Yes, we could yes go ahead and } prep some samples, but rather than waste the time and effort I } thought I'd get the feeling from all you folks: (1) Garbage?, (2) } Likely to be good?, (3) I don't know have to test? } } Thanks! } } (I know it reminds me of that crystal OsO4 find a little while ago). } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } "RAM disk is NOT an installation procedure." } } } ==============================Original } Headers============================== } 6, 22 -- From edelmare-at-muohio.edu Thu Dec 11 13:23:56 2008 } 6, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu } [134.53.6.69]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id mBBJNuXH021007 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 } 13:23:56 -0600 } 6, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu } [134.53.6.11]) } 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id mBBJO375022156 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 } 14:24:03 -0500 } 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) } 6, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id mBBJNtxf014380 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 } 14:23:55 -0500 } 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } 6, 22 -- To: microscopy-at-Microscopy.com } 6, 22 -- Date: Thu, 11 Dec 2008 14:23:55 -0500 } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- Subject: Shelf life of Glutaraldehyde } 6, 22 -- Message-ID: {4941227B.1083.6316340-at-edelmare.muohio.edu} } 6, 22 -- Priority: normal } 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) } 6, 22 -- Content-type: text/plain; charset=US-ASCII } 6, 22 -- Content-transfer-encoding: 7BIT } 6, 22 -- Content-description: Mail message body } 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69 } ==============================End of - } Headers============================== } } } ==============================Original } Headers============================== } 16, 29 -- From PhillipsT-at-missouri.edu Thu Dec 11 13:40:16 2008 } 16, 29 -- Received: from mxtip01-missouri-out.um.umsystem.edu } (mxtip01-missouri-out.um.umsystem.edu [209.106.229.45]) } 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mBBJeFRH002456 } 16, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 } 13:40:16 -0600 } 16, 29 -- X-IronPort-Anti-Spam-Filtered: true } 16, 29 -- X-IronPort-Anti-Spam-Result: ApoEAPT6QEnRauUp/ } 2dsb2JhbADDCwEJhkGFMAGBcoEG } 16, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) } ([209.106.229.41]) } 16, 29 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 11 } Dec 2008 13:40:15 -0600 } 16, 29 -- Received: from UM-XMAIL06.um.umsystem.edu } ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft } SMTPSVC(6.0.3790.3959); } 16, 29 -- Thu, 11 Dec 2008 13:40:15 -0600 } 16, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 16, 29 -- Content-class: urn:content-classes:message } 16, 29 -- MIME-Version: 1.0 } 16, 29 -- Content-Type: text/plain; } 16, 29 -- charset="us-ascii" } 16, 29 -- Subject: RE: [Microscopy] Shelf life of Glutaraldehyde } 16, 29 -- Date: Thu, 11 Dec 2008 13:39:53 -0600 } 16, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD059022C7-at-UM-XMAIL06.um.umsystem.edu } } } 16, 29 -- In-Reply-To: {200812111925.mBBJP3qN022143-at-ns.microscopy.com} } 16, 29 -- X-MS-Has-Attach: } 16, 29 -- X-MS-TNEF-Correlator: } 16, 29 -- Thread-Topic: [Microscopy] Shelf life of Glutaraldehyde } 16, 29 -- Thread-Index: AclbxiuZ6TpJRbVRSiGzNzHumWAkfAAAaANg } 16, 29 -- References: {200812111925.mBBJP3qN022143-at-ns.microscopy.com} } 16, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 16, 29 -- To: {Microscopy-at-microscopy.com} } 16, 29 -- X-OriginalArrivalTime: 11 Dec 2008 19:40:15.0492 (UTC) } FILETIME=[4A4D0C40:01C95BC8] } 16, 29 -- Content-Transfer-Encoding: 8bit } 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id mBBJeFRH002456 } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 26 -- From glenmac-at-u.washington.edu Thu Dec 11 13:56:06 2008 4, 26 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJu6Ul016299 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 13:56:06 -0600 4, 26 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.33.9] (may be forged)) 4, 26 -- by mxout2.cac.washington.edu (8.13.7+UW06.06/8.13.7+UW07.09) with ESMTP id mBBJu5F7029262 4, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 11:56:05 -0800 4, 26 -- X-Auth-Received: from D-128-95-178-137.dhcp4.washington.edu (D-128-95-178-137.dhcp4.washington.edu [128.95.178.137]) 4, 26 -- (authenticated authid=glenmac) 4, 26 -- by smtp.washington.edu (8.14.3+UW08.09/8.14.3+UW08.11) with ESMTP id mBBJu5qi011922 4, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 4, 26 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 11:56:05 -0800 4, 26 -- Message-Id: {1E00A063-7D91-4313-8DE2-A45BC7F144E8-at-u.washington.edu} 4, 26 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 4, 26 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 4, 26 -- In-Reply-To: {200812111941.mBBJfOA5004513-at-ns.microscopy.com} 4, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 26 -- Content-Transfer-Encoding: 7bit 4, 26 -- Mime-Version: 1.0 (Apple Message framework v929.2) 4, 26 -- Subject: Re: [Microscopy] RE: Shelf life of Glutaraldehyde 4, 26 -- Date: Thu, 11 Dec 2008 11:56:05 -0800 4, 26 -- References: {200812111941.mBBJfOA5004513-at-ns.microscopy.com} 4, 26 -- X-Mailer: Apple Mail (2.929.2) 4, 26 -- X-PMX-Version: 5.5.0.356843, Antispam-Engine: 2.6.1.350677, Antispam-Data: 2008.12.11.194317 4, 26 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=8%, Report='__BOUNCE_CHALLENGE_SUBJ 0, __C230066_P5 0, __CP_AGE_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __KNOWN_PHONE_RUSSIA_COUNTRY_CODE7_PREFIX8 0, __KNOWN_PHONE_RU_812 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_6 0' ==============================End of - Headers==============================
We prefer to use fresh glut. - usually 1 yr old max but sometimes it's a bit older - I always ask the person fixing the tissue if they care or not. Some older faculty actually preferred really old glut for their marine samples - not sure why.
And, I agree, It seems the age of the osmium doesn't ever seem to matter - it's always good.
best, Beth
On Dec 11, 2008, at 2:24 PM, edelmare-at-muohio.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } O.k. just found 20 sealed ampoules of 10ml 50% EM Grade } glutaraldehyde. Stored in a refrigerator, purchase from one of the } EM supply companies (so not some unknown quality vendor). } } BUT they were purchased in 1994. Yes, we could yes go ahead and } prep some samples, but rather than waste the time and effort I } thought I'd get the feeling from all you folks: (1) Garbage?, (2) } Likely to be good?, (3) I don't know have to test? } } Thanks! } } (I know it reminds me of that crystal OsO4 find a little while ago). } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu } } "RAM disk is NOT an installation procedure." } } } ==============================Original } Headers============================== } 6, 22 -- From edelmare-at-muohio.edu Thu Dec 11 13:23:56 2008 } 6, 22 -- Received: from mulnx11.mcs.muohio.edu } (mulnx11.mcs.muohio.edu [134.53.6.69]) } 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mBBJNuXH021007 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 13:23:56 } -0600 } 6, 22 -- Received: from mulnx24.mcs.muohio.edu } (mulnx24.mcs.muohio.edu [134.53.6.11]) } 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with } ESMTP id mBBJO375022156 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:24:03 } -0500 } 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) } 6, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with } ESMTP id mBBJNtxf014380 } 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:23:55 } -0500 } 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } 6, 22 -- To: microscopy-at-Microscopy.com } 6, 22 -- Date: Thu, 11 Dec 2008 14:23:55 -0500 } 6, 22 -- MIME-Version: 1.0 } 6, 22 -- Subject: Shelf life of Glutaraldehyde } 6, 22 -- Message-ID: {4941227B.1083.6316340-at-edelmare.muohio.edu} } 6, 22 -- Priority: normal } 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) } 6, 22 -- Content-type: text/plain; charset=US-ASCII } 6, 22 -- Content-transfer-encoding: 7BIT } 6, 22 -- Content-description: Mail message body } 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69 } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 20 -- From beth-at-plantbio.uga.edu Thu Dec 11 13:57:19 2008 7, 20 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJvJwt018905 7, 20 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 13:57:19 -0600 7, 20 -- Received: from [128.192.26.46] ([128.192.26.46]) 7, 20 -- (authenticated user beth-at-plantbio.uga.edu) 7, 20 -- by dogwood.plantbio.uga.edu 7, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 7, 20 -- Thu, 11 Dec 2008 14:57:12 -0500 7, 20 -- Message-Id: {AAAADAC0-CE20-4A51-9182-4D0F1D16A3AB-at-plantbio.uga.edu} 7, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 7, 20 -- To: edelmare-at-muohio.edu, microscopy microscopy {microscopy-at-microscopy.com} 7, 20 -- In-Reply-To: {200812111924.mBBJOs3e021928-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 7, 20 -- Content-Transfer-Encoding: 7bit 7, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 7, 20 -- Subject: Re: [Microscopy] Shelf life of Glutaraldehyde 7, 20 -- Date: Thu, 11 Dec 2008 14:57:15 -0500 7, 20 -- References: {200812111924.mBBJOs3e021928-at-ns.microscopy.com} 7, 20 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I would not use 3 year-old glut. I had a sad experience of doing perfusion-fixations using fixative containing 3 year-old 50% glut from a respected EM vendor, in sealed amber ampoules, stored at-20C (it does not freeze at this temp). The fixative solution was formulated properly and tested correctly for pH and osmolarity. The animals had been on a 6 or 9 week protocol- thus expensive animals. The perfusion was excellent, judged by the open lumens in the kidneys, but the cellular fixation was terrible. Unusable. The glut had no expiration date on it, but we called the vendor and they told us at the time that the shelf life was approximately 14-16 mos. Now I think they are saying 2 years shelf life.
My advice is: Don't take a chance and don't waste your time and resources testing it. Glut is cheap. Labor, animals, time, other supplies are very expensive.
Best regards,
Jill
Jill Verlander Reed, DVM Associate Scientist Division of Nephrology, Hypertension, and Transplantation and Department of Anatomy and Cell Biology Director, College of Medicine Electron Microscopy Core Facility University of Florida College of Medicine P.O. Box 100224 HSC 1600 SW Archer Road Gainesville, FL 32610-0224 phone (352) 846-0820 fax (352) 392-8996
==============================Original Headers============================== 7, 38 -- From Jill.Verlander-at-medicine.ufl.edu Thu Dec 11 13:59:04 2008 7, 38 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 7, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJx30o024737 7, 38 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 13:59:04 -0600 7, 38 -- Received: from medexchange01.ad.medicine.ufl.edu (medexchange01.medicine.ufl.edu [159.178.41.17]) 7, 38 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id mBBJx2g4024607 7, 38 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT) 7, 38 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 14:59:02 -0500 7, 38 -- Received: from medexchange02.ad.medicine.ufl.edu ([159.178.40.106]) by 7, 38 -- medexchange01.ad.medicine.ufl.edu ([159.178.41.17]) with mapi; Thu, 11 Dec 7, 38 -- 2008 14:59:20 -0500 7, 38 -- From: "Verlander, Jill" {Jill.Verlander-at-medicine.ufl.edu} 7, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 38 -- Date: Thu, 11 Dec 2008 14:59:16 -0500 7, 38 -- Subject: shelf life of glut 7, 38 -- Thread-Topic: shelf life of glut 7, 38 -- Thread-Index: AclbyvJQndAb56xfRnaj7G/OLvIvTQ== 7, 38 -- Message-ID: {093B4ED6F713124284B69C0F525376511AE2FBB9AD-at-medexchange02.ad.medicine.ufl.edu} 7, 38 -- Accept-Language: en-US 7, 38 -- Content-Language: en-US 7, 38 -- X-MS-Has-Attach: 7, 38 -- X-MS-TNEF-Correlator: 7, 38 -- x-cr-hashedpuzzle: yS0= A6MW CLim DBFX DLJa DmVd EUb+ FXBl FgMN GBG7 HNor 7, 38 -- H6ev JTO/ KK4z L9m7 7, 38 -- Md9z;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{C080F5A6-3AAE-4716-9875-3E93CAA7BA99};agBpAGwAbAAuAHYAZQByAGwAYQBuAGQAZQByAEAAbQBlAGQAaQBjAGkAbgBlAC4AdQBmAGwALgBlAGQAdQA=;Thu, 7, 38 -- 11 Dec 2008 19:59:16 GMT;cwBoAGUAbABmACAAbABpAGYAZQAgAG8AZgAgAGcAbAB1AHQA 7, 38 -- x-cr-puzzleid: {C080F5A6-3AAE-4716-9875-3E93CAA7BA99} 7, 38 -- acceptlanguage: en-US 7, 38 -- Content-Type: text/plain; charset="us-ascii" 7, 38 -- MIME-Version: 1.0 7, 38 -- X-Virus-Scanned: ClamAV version 0.92, clamav-milter version 0.92 on mailfilter05.cns.ufl.edu 7, 38 -- X-Virus-Status: Clean 7, 38 -- X-Spam-Status: hits=-3, required=5, tests=ALL_TRUSTED 7, 38 -- X-UFL-Spam-Status: hits=-3, required=5, tests=ALL_TRUSTED 7, 38 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 7, 38 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 7, 38 -- Content-Transfer-Encoding: 8bit 7, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBJx30o024737 ==============================End of - Headers==============================
Purists will hate this reply, but we often delegate questionable chemicals for non-critical SEM work or for re-do-able protocol trials. For critical work (purists would say it's ALL critical), we stay within expiration dates---at least when expiration dates are available.
Another example of this would be using recycled chemicals from our Environmental Health and Safety Department for trial runs, but switching to new stuff for paying customers with Great Expectations. Especially when grant deadlines are near.
My two tambala worth.
Cheers, Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan Sons of Norway: http://www.sofn.com/
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Thursday, December 11, 2008 1:25 PM To: Tindall, Randy D.
O.k. just found 20 sealed ampoules of 10ml 50% EM Grade glutaraldehyde. Stored in a refrigerator, purchase from one of the EM supply companies (so not some unknown quality vendor).
BUT they were purchased in 1994. Yes, we could yes go ahead and prep some samples, but rather than waste the time and effort I thought I'd get the feeling from all you folks: (1) Garbage?, (2) Likely to be good?, (3) I don't know have to test?
Thanks!
(I know it reminds me of that crystal OsO4 find a little while ago). Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 6, 22 -- From edelmare-at-muohio.edu Thu Dec 11 13:23:56 2008 6, 22 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.69]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJNuXH021007 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 13:23:56 -0600 6, 22 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 6, 22 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBJO375022156 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:24:03 -0500 6, 22 -- Received: from [192.168.1.23] ([134.53.14.105]) 6, 22 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBJNtxf014380 6, 22 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:23:55 -0500 6, 22 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 6, 22 -- To: microscopy-at-Microscopy.com 6, 22 -- Date: Thu, 11 Dec 2008 14:23:55 -0500 6, 22 -- MIME-Version: 1.0 6, 22 -- Subject: Shelf life of Glutaraldehyde 6, 22 -- Message-ID: {4941227B.1083.6316340-at-edelmare.muohio.edu} 6, 22 -- Priority: normal 6, 22 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 22 -- Content-type: text/plain; charset=US-ASCII 6, 22 -- Content-transfer-encoding: 7BIT 6, 22 -- Content-description: Mail message body 6, 22 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.69 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 30 -- From TindallR-at-missouri.edu Thu Dec 11 14:11:48 2008 21, 30 -- Received: from mxtip01-missouri-out.um.umsystem.edu (mxtip01-missouri-out.um.umsystem.edu [209.106.229.45]) 21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBKBmDd024325 21, 30 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 14:11:48 -0600 21, 30 -- X-IronPort-Anti-Spam-Filtered: true 21, 30 -- X-IronPort-Anti-Spam-Result: ApoEACEDQUnRauUp/2dsb2JhbADCdgEJhkGFMIFzgQaDcw 21, 30 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 21, 30 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 11 Dec 2008 14:11:48 -0600 21, 30 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 21, 30 -- Thu, 11 Dec 2008 14:11:47 -0600 21, 30 -- x-mimeole: Produced By Microsoft Exchange V6.5 21, 30 -- Content-class: urn:content-classes:message 21, 30 -- MIME-Version: 1.0 21, 30 -- Content-Type: text/plain; 21, 30 -- charset="US-ASCII" 21, 30 -- Subject: RE: [Microscopy] Shelf life of Glutaraldehyde 21, 30 -- Date: Thu, 11 Dec 2008 14:10:57 -0600 21, 30 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7C69-at-UM-XMAIL08.um.umsystem.edu} 21, 30 -- In-Reply-To: {200812111925.mBBJPMaO022635-at-ns.microscopy.com} 21, 30 -- X-MS-Has-Attach: 21, 30 -- X-MS-TNEF-Correlator: 21, 30 -- Thread-Topic: [Microscopy] Shelf life of Glutaraldehyde 21, 30 -- Thread-Index: Aclbxjijx0sR/MdpTDiQBKDuTCNiIgABVh6g 21, 30 -- References: {200812111925.mBBJPMaO022635-at-ns.microscopy.com} 21, 30 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 21, 30 -- To: {edelmare-at-muohio.edu} 21, 30 -- Cc: {microscopy-at-microscopy.com} 21, 30 -- X-OriginalArrivalTime: 11 Dec 2008 20:11:47.0902 (UTC) FILETIME=[B243E5E0:01C95BCC] 21, 30 -- Content-Transfer-Encoding: 8bit 21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBKBmDd024325 ==============================End of - Headers==============================
That was exactly my thoughts too! But looking for some real experiences rather than gut reaction.
Thanks!
On 11 Dec 2008 at 14:59, Jill.Verlander-at-medicine.ufl.e wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I would not use 3 year-old glut. I had a sad experience of doing perfusion-fixations using fixative containing 3 year-old 50% glut from a respected EM vendor, in sealed amber ampoules, stored at-20C (it does not freeze at this temp). The fixative solution was formulated properly and tested correctly for pH and osmolarity. The animals had been on a 6 or 9 week protocol- thus expensive animals. The perfusion was excellent, judged by the open lumens in the kidneys, but the cellular fixation was terrible. Unusable. The glut had no expiration date on it, but we called the vendor and they told us at the time that the shelf life was approximately 14-16 mos. Now I think they are saying 2 years shelf life. } } My advice is: Don't take a chance and don't waste your time and resources testing it. Glut is cheap. Labor, animals, time, other supplies are very expensive. } } Best regards, } } Jill } } Jill Verlander Reed, DVM } Associate Scientist } Division of Nephrology, Hypertension, and Transplantation and } Department of Anatomy and Cell Biology } Director, College of Medicine Electron Microscopy Core Facility } University of Florida College of Medicine } P.O. Box 100224 HSC } 1600 SW Archer Road } Gainesville, FL 32610-0224 } phone (352) 846-0820 } fax (352) 392-8996 } } } } ==============================Original Headers============================== } 7, 38 -- From Jill.Verlander-at-medicine.ufl.edu Thu Dec 11 13:59:04 2008 } 7, 38 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) } 7, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBJx30o024737 } 7, 38 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 13:59:04 -0600 } 7, 38 -- Received: from medexchange01.ad.medicine.ufl.edu (medexchange01.medicine.ufl.edu [159.178.41.17]) } 7, 38 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id mBBJx2g4024607 } 7, 38 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT) } 7, 38 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 14:59:02 -0500 } 7, 38 -- Received: from medexchange02.ad.medicine.ufl.edu ([159.178.40.106]) by } 7, 38 -- medexchange01.ad.medicine.ufl.edu ([159.178.41.17]) with mapi; Thu, 11 Dec } 7, 38 -- 2008 14:59:20 -0500 } 7, 38 -- From: "Verlander, Jill" {Jill.Verlander-at-medicine.ufl.edu} } 7, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 7, 38 -- Date: Thu, 11 Dec 2008 14:59:16 -0500 } 7, 38 -- Subject: shelf life of glut } 7, 38 -- Thread-Topic: shelf life of glut } 7, 38 -- Thread-Index: AclbyvJQndAb56xfRnaj7G/OLvIvTQ== } 7, 38 -- Message-ID: {093B4ED6F713124284B69C0F525376511AE2FBB9AD-at-medexchange02.ad.medicine.ufl.edu} } 7, 38 -- Accept-Language: en-US } 7, 38 -- Content-Language: en-US } 7, 38 -- X-MS-Has-Attach: } 7, 38 -- X-MS-TNEF-Correlator: } 7, 38 -- x-cr-hashedpuzzle: yS0= A6MW CLim DBFX DLJa DmVd EUb+ FXBl FgMN GBG7 HNor } 7, 38 -- H6ev JTO/ KK4z L9m7 } 7, 38 -- Md9z;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{C080F5A6-3AAE-4716-9875-3E93CAA7BA99};agBpAGwAbAAuAHYAZQByAGwAYQBuAGQAZQByAEAAbQBlAGQAaQBjAGkAbgBlAC4AdQBmAGwALgBlAGQAdQA=;Thu, } 7, 38 -- 11 Dec 2008 19:59:16 GMT;cwBoAGUAbABmACAAbABpAGYAZQAgAG8AZgAgAGcAbAB1AHQA } 7, 38 -- x-cr-puzzleid: {C080F5A6-3AAE-4716-9875-3E93CAA7BA99} } 7, 38 -- acceptlanguage: en-US } 7, 38 -- Content-Type: text/plain; charset="us-ascii" } 7, 38 -- MIME-Version: 1.0 } 7, 38 -- X-Virus-Scanned: ClamAV version 0.92, clamav-milter version 0.92 on mailfilter05.cns.ufl.edu } 7, 38 -- X-Virus-Status: Clean } 7, 38 -- X-Spam-Status: hits=-3, required=5, tests=ALL_TRUSTED } 7, 38 -- X-UFL-Spam-Status: hits=-3, required=5, tests=ALL_TRUSTED } 7, 38 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) } 7, 38 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) } 7, 38 -- Content-Transfer-Encoding: 8bit } 7, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBJx30o024737 } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"RAM disk is NOT an installation procedure."
==============================Original Headers============================== 9, 25 -- From edelmare-at-muohio.edu Thu Dec 11 14:47:03 2008 9, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.70]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBKl2lf006366 9, 25 -- for {microscopy-at-Microscopy.com} ; Thu, 11 Dec 2008 14:47:02 -0600 9, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 9, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBKl0DB029263; 9, 25 -- Thu, 11 Dec 2008 15:47:00 -0500 9, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 9, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id mBBKl0Xg011038; 9, 25 -- Thu, 11 Dec 2008 15:47:00 -0500 9, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 9, 25 -- To: "Jill.Verlander-at-medicine.ufl.edu" {Jill.Verlander-at-medicine.ufl.edu} 9, 25 -- Date: Thu, 11 Dec 2008 15:46:59 -0500 9, 25 -- MIME-Version: 1.0 9, 25 -- Subject: Re: [Microscopy] shelf life of glut 9, 25 -- CC: microscopy-at-Microscopy.com 9, 25 -- Message-ID: {494135F3.7983.67D6FE1-at-edelmare.muohio.edu} 9, 25 -- Priority: normal 9, 25 -- In-reply-to: {200812111959.mBBJxvmg027728-at-ns.microscopy.com} 9, 25 -- References: {200812111959.mBBJxvmg027728-at-ns.microscopy.com} 9, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 25 -- Content-type: text/plain; charset=US-ASCII 9, 25 -- Content-transfer-encoding: 7BIT 9, 25 -- Content-description: Mail message body 9, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.70 ==============================End of - Headers==============================
Looking back it only seems like I trained with Methuselah. I guess the late '60s were really not the dark ages. While we were rather careful, clearly our opinion of acceptability is less than today's. We used buy our glut in 1-4L containers and purify it ourselves. Treat with activated charcoal or Barium Carbonate, which adsorbed the glutaric acid and gave us a relatively clean sample as shown by measuring absorbance. Always worked, never had a problem. If there were problems Jill, we could always find a far more reasonable explanation. Sometimes we didn't like the explanations because they said we did it wrong, but they were always more reasonable. In this case, we means I, since I was (and still am) responsible for the our small lab, and ultimately for all work that comes through.
Studies on purity of glutaraldehyde at the time were also very discordant. Some said it had to be fresh distilled, others showed the presence of a little impurity (as shown by the presence of additional absorbance peaks [other than max at wavelength 280]) actually improved fixation (probably why some of Beth's users in Georgia want old glut - not because it makes a difference, but that is their version of dogma). Among the things which did come out of the old studies were that high concentration, storage at low temperature, and storage under nitrogen gas all were positive factors for stability over very long periods of time. When you think about it, this is actually very logical. Reducing the temperature slows the rate of reaction, and removing oxygen removes one of the essential ingredients for the aldehyde to acid reaction.
About 15 years ago, as fixing, embedding and thin sectioning became less and less of an issue for me, I went to purchasing 5 and 10ml 50% brown glass ampules of EM grade glutaraldehyde stored under nitrogen. I store it at -20, and have yet to see any problem. Of course, it is still used up by the end of 4-5 years max. I would not be too concerned if it was older. So I would go for it Tom.
Anyway, Richard, if you want to be safe, do as Randy said - use what you found in the fridge on materials of lesser importance, and purchase small lots of 25-50% EM grade ampules under nitrogen every 12-18 months and store at in the freezer.purified material.
Paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 William Avenue Winnipeg, Manitoba, Canada, R3E 0J9 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 9, 20 -- From paul_hazelton-at-umanitoba.ca Thu Dec 11 15:15:59 2008 9, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBLFxqm020510 9, 20 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 15:15:59 -0600 9, 20 -- Received: from [140.193.25.69] (basic069.medmb.umanitoba.ca [140.193.25.69]) 9, 20 -- (authenticated bits=0) 9, 20 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id mBBLFthO004581; 9, 20 -- Thu, 11 Dec 2008 15:15:57 -0600 (CST) 9, 20 -- Message-ID: {4941830B.1080403-at-umanitoba.ca} 9, 20 -- Date: Thu, 11 Dec 2008 15:15:55 -0600 9, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 9, 20 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 9, 20 -- MIME-Version: 1.0 9, 20 -- To: edelmare-at-muohio.edu, Microscopy Listserver {microscopy-at-microscopy.com} 9, 20 -- Subject: Re: [Microscopy] Shelf life of Glutaraldehyde 9, 20 -- References: {200812111926.mBBJQQ6u023927-at-ns.microscopy.com} 9, 20 -- In-Reply-To: {200812111926.mBBJQQ6u023927-at-ns.microscopy.com} 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 20 -- Content-Transfer-Encoding: 7bit 9, 20 -- X-DCC-UofM-Metrics: taygeta; whitelist ==============================End of - Headers==============================
Realistically I'd use it for a non critical study to check it out. #2 Likely good. I have used some old glut. but not that old. When I started in TEM I was told to use a bottle of 50% from Fisher- pint size! It had been in use for at least a year and I used it for another year if I remember right. It worked, although it gives me shivers now when I think of doing it.
I agree that if the glut. is not perfectly pure it gives better results than the purest type. Sort of like using a combination of Paraformaldehyde and Glutaraldehyde to fix at different speeds. I learned that the fastest fixatives are the ones that do not fix the best but the best ones take a long time to start working. I'm sure someone will enlighten me if this is not correct.
You might also contact the vendor who's name is on the supply and ask their opinion.
Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} From: {edelmare-at-muohio.edu} } Reply-To: {edelmare-at-muohio.edu} } Date: Thu, 11 Dec 2008 13:30:24 -0600 } To: {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] Shelf life of Glutaraldehyde } } O.k. just found 20 sealed ampoules of 10ml 50% EM Grade } glutaraldehyde. Stored in a refrigerator, purchase from one of the } EM supply companies (so not some unknown quality vendor). } } BUT they were purchased in 1994. Yes, we could yes go ahead and } prep some samples, but rather than waste the time and effort I } thought I'd get the feeling from all you folks: (1) Garbage?, (2) } Likely to be good?, (3) I don't know have to test? } } Thanks! } } (I know it reminds me of that crystal OsO4 find a little while ago). } Richard E. Edelmann, Ph.D. } Electron Microscopy Facility Director } 364 Pearson Hall } Miami University, Oxford, OH 45056 } Ph: 513.529.5712 Fax: 513.529.4243 } E-mail: edelmare-at-muohio.edu } http://www.emf.muohio.edu
==============================Original Headers============================== 12, 27 -- From connellyps-at-nhlbi.nih.gov Thu Dec 11 17:25:34 2008 12, 27 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBBNPY4U005151 12, 27 -- for {microscopy-at-microscopy.com} ; Thu, 11 Dec 2008 17:25:34 -0600 12, 27 -- X-IronPortListener: Outbound_SMTP 12, 27 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) 12, 27 -- by nihxway4out.hub.nih.gov with ESMTP; 11 Dec 2008 17:01:42 -0500 12, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); 12, 27 -- Thu, 11 Dec 2008 17:01:38 -0500 12, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.161]) with Microsoft Exchange Server HTTP-DAV ; 12, 27 -- Thu, 11 Dec 2008 22:01:37 +0000 12, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 12, 27 -- Date: Thu, 11 Dec 2008 17:01:03 -0500 12, 27 -- Subject: Re: [Microscopy] Shelf life of Glutaraldehyde 12, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 12, 27 -- To: {edelmare-at-muohio.edu} , 12, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 27 -- Message-ID: {C566F7CF.2B54%connellyps-at-nhlbi.nih.gov} 12, 27 -- Thread-Topic: [Microscopy] Shelf life of Glutaraldehyde 12, 27 -- Thread-Index: Aclb2/VoNCnIrsfPEd2euAANk2Yv1A== 12, 27 -- In-Reply-To: {200812111930.mBBJUODU028638-at-ns.microscopy.com} 12, 27 -- Mime-version: 1.0 12, 27 -- Content-type: text/plain; 12, 27 -- charset="ISO-8859-1" 12, 27 -- X-OriginalArrivalTime: 11 Dec 2008 22:01:38.0038 (UTC) FILETIME=[0A4AD960:01C95BDC] 12, 27 -- Content-Transfer-Encoding: 8bit 12, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBBNPY4U005151 ==============================End of - Headers==============================
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I'm looking for "ergonomic" tables for our light microscopes. We don't need vibration-isolation, just something comfortable to sit at, maybe with armrests, adjustable height or surface angle, etc. Do any of you have a table that you like and could recommend?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT Dublin
Title-Subject: [Filtered] Info for disposal of JEOL T200 SEM
Question: Hello listers.
I am about to dispose of a JEOL T200 SEM. In order to do so, I need to know the quantities of heavy metals and any other hazardous substances etc associated with it. The waste disposal folk are requesting MSDS for same. As long as I have been in this institute, this instrument has sat in a corner, unused. There is no manual, nothing etc for it. I will endeavour to get the required information from JEOL, of course, but if anyone has any advice or information, I'd be grateful. Regards Emer.
If you get any definitive or procedural info on this subject in general, I for one would really appreciate knowing about it and the details.
I think this is a very tricky subject based on the technology foundation of the SEM at its time of manufacture. Certainly there are other variables. But either way, this could be a big problem for someone wanting to dispose of a SEM or a TEM.
gary g.
At 01:42 PM 12/12/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Sat Dec 13 01:23:09 2008 11, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBD7N8Ko002998 11, 20 -- for {microscopy-at-microscopy.com} ; Sat, 13 Dec 2008 01:23:09 -0600 11, 20 -- Message-Id: {200812130723.mBD7N8Ko002998-at-ns.microscopy.com} 11, 20 -- Received: (qmail 23054 invoked from network); 12 Dec 2008 23:21:09 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 23051, pid: 23052, t: 0.0696s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp2 with SMTP; 12 Dec 2008 23:21:09 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Fri, 12 Dec 2008 23:23:02 -0800 11, 20 -- To: emer.ryan-at-dit.ie 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] viaWWW: Info for disposal of JEOL T200 SEM 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200812122142.mBCLgNNQ003163-at-ns.microscopy.com} 11, 20 -- References: {200812122142.mBCLgNNQ003163-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
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Email: swtkeller-at-yahoo.com Name: Sandra Keller
Organization: TAS-SICCO
Title-Subject: [Filtered] TEM- Looking for a used Gatan Dimple Grinder (complete)
Question: Hi All: I am looking for a later generation used Gatan Dimple Grinder (ie: the generation that has the ability to light from both bottom and top) and would also have a microscope to center the specimen. Perhaps someone has one lying around and would like to part with it for a reasonable price? Please let me know, Sandra
I am looking for someone who has a Cell Robotics laser micro-dissection system, or who knows how one is set up.
We have a system that needs setting up and someone "borrowed" the instruction manual. If anyone who sees this message thinks they could assist us, please contact me off-line and we can work something out. At the very least, a copy of the instructions would be very helpful.
Many thanks,
Paul Webster
2100 W 3rd St Los Angeles, CA 90057.
==============================Original Headers============================== 8, 20 -- From PWebster-at-hei.org Sat Dec 13 01:51:55 2008 8, 20 -- Received: from hi0sml1.hei.org (heimail.hei.org [12.88.48.54] (may be forged)) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBD7psKm030201 8, 20 -- for {microscopy-at-Microscopy.com} ; Sat, 13 Dec 2008 01:51:55 -0600 8, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 20 -- Content-class: urn:content-classes:message 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- Subject: Cell Robotics 8, 20 -- Date: Fri, 12 Dec 2008 23:51:54 -0800 8, 20 -- Message-ID: {87449E4A2B01DA47B29424CE5D6E0F830814CC3D-at-hi0sml1.hei.org} 8, 20 -- X-MS-Has-Attach: 8, 20 -- X-MS-TNEF-Correlator: 8, 20 -- Thread-Topic: Cell Robotics 8, 20 -- Thread-Index: Aclc96o8rnTX/i+wTMu5ppXCHu63Iw== 8, 20 -- From: "Webster, Paul" {PWebster-at-hei.org} 8, 20 -- To: {microscopy-at-Microscopy.com} 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBD7psKm030201 ==============================End of - Headers==============================
If you recycle it, it is exempt from the waste regulations (RCRA in 40 CFR 260-265).
You could send it to a metals recycler or circuit board/computer recycler. The latter of these two extract the higher end metals whereas the former will lump it together.
In either case, the recycling shipping papers should be maintained for 5 years in the event of a question. Typically, the recycler's regulatory history should be checked so you don't end up on a hazwaste site list as a potentially responsible party (PRP).
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon, IN 46123 www.ph2llc.com
(317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
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-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Saturday, December 13, 2008 2:28 AM To: ph2-at-sprynet.com
If you get any definitive or procedural info on this subject in general, I for one would really appreciate knowing about it and the details.
I think this is a very tricky subject based on the technology foundation of the SEM at its time of manufacture. Certainly there are other variables. But either way, this could be a big problem for someone wanting to dispose of a SEM or a TEM.
gary g.
At 01:42 PM 12/12/2008, you wrote:
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==============================Original Headers============================== 26, 28 -- From ph2-at-sprynet.com Sat Dec 13 08:04:00 2008 26, 28 -- Received: from elasmtp-spurfowl.atl.sa.earthlink.net (elasmtp-spurfowl.atl.sa.earthlink.net [209.86.89.66]) 26, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBDE3xpK021630 26, 28 -- for {microscopy-at-microscopy.com} ; Sat, 13 Dec 2008 08:04:00 -0600 26, 28 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 26, 28 -- s=dk20050327; d=sprynet.com; 26, 28 -- b=Vlpx0GXhwjS+Fv651HWGgnjrWt+fzMPn2YMXjLaVvyIaNSzQfX3bMIshGa5uXbJa; 26, 28 -- h=Received:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:In-Reply-To:X-MimeOLE:Message-ID:X-ELNK-Trace:X-Originating-IP; 26, 28 -- Received: from [68.21.95.86] (helo=user915fa8f284) 26, 28 -- by elasmtp-spurfowl.atl.sa.earthlink.net with esmtpa (Exim 4.67) 26, 28 -- (envelope-from {ph2-at-sprynet.com} ) 26, 28 -- id 1LBV67-0001t3-04 26, 28 -- for microscopy-at-microscopy.com; Sat, 13 Dec 2008 09:03:59 -0500 26, 28 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 26, 28 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 26, 28 -- Subject: RE: [Microscopy] Re: viaWWW: Info for disposal of JEOL T200 SEM 26, 28 -- Date: Sat, 13 Dec 2008 09:03:35 -0500 26, 28 -- MIME-Version: 1.0 26, 28 -- Content-Type: text/plain; 26, 28 -- charset="us-ascii" 26, 28 -- Content-Transfer-Encoding: 7bit 26, 28 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 26, 28 -- Thread-Index: Aclc9FS5I30qY3zYR7SDet6V6SnJzQANlmow 26, 28 -- In-Reply-To: {200812130728.mBD7S2UY010245-at-ns.microscopy.com} 26, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3350 26, 28 -- Message-ID: {E1LBV67-0001t3-04-at-elasmtp-spurfowl.atl.sa.earthlink.net} 26, 28 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9a7748dea729f3fd9596d8ce4b75e80e0350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 26, 28 -- X-Originating-IP: 68.21.95.86 ==============================End of - Headers==============================
Bryan, Thanks for pointing that out. Basically, I'm not at all familiar with any of the PC operated models, although I'm a little surprised that they're mixing and matching columns now.
Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: bbandli-at-d.umn.edu [mailto:bbandli-at-d.umn.edu] Sent: Monday, December 08, 2008 2:26 PM To: kenconverse-at-qualityimages.biz
Hi All,
It might be important to keep track of JEOL's non-chronological model numbering in this discussion.
As Ken mentioned below, the 6400 is not run by a PC (which is correct), however, the "6xxx models" having an 840 column and vacuum system is a bit inaccurate. The 6490LV I have is not an 840 (or a 6400 for that matter). It is more similar to the 5900. There are significant chronological discontinuities in the JEOL numbering for their tungsten filament SEM models (i.e. the 5900 is a newer model than the 6400). I'm no expert on which model is "newer", but I do know it does get a bit confusing trying to keep track of all the changes.
Hopefully I haven't done anything to add confusion here.
But, to add to the thread, I have a 6490LV and agree with previous comments on it. The PC interface is good, but does have some room for improvement. I have the optional LVSE ("Low Vacuum Secondary Electron") detector and have only had a few opportunities to use it, and with some work it does produce good images. I haven't run any of the earlier JEOL VP instruments so I can't speak about them.
Good Luck! Bryan Bandli
kenconverse-at-qualityimages.biz wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Larry, } I don't know about the newer 6xxx models, but the 6300/6400 are not run by a } PC which means I can probably keep them running for a long time. There was } a thread about a couple of months ago concerning PC operated SEMs. You may } gain flexibility with the PC, but the basic SEM is good to go for 20-40 } years, while the PC is, by definition, obsolete before it is installed. } Currently, upgrades of PCs (when available) run in the range of $25k. You } can't just run down to Best Buy and buy a new computer to replace your 80486 } running Win 3.1. It ain't gonna work. } } As to the column, the 6xxx series has basically an 840 column and vacuum } system which bears no resemblance whatsoever to the 5200, 5300 or 5400 } (which are very similar to the T2xx/T3xx series). I can't vouch for the } 5800. } }
} Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: hanke-at-mee-inc.com [mailto:hanke-at-mee-inc.com] } Sent: Sunday, December 07, 2008 2:58 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Re: JEOL SEM's: comments on 5000 series vs 6000 } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have had a JEOL 5800LV for almost 14 years. It gets a ton of work in } the VP mode and works very well. Imaging is limited to the backscattered } detector in the VP mode, and as Gary noted, resolution is not great at } 10K and greater magnification in VP mode. But this instrument performs } very well at high vacuum. I do not think that there is any decrease in } performance in high vacuum mode compared to a non-VP instrument. Imaging } at 50KX is possible and I have worked at voltages as low as 2 KV. Not } bad for a tungsten source. } } I have limited experience with the 6000 series instruments. I think that } the column and detectors were very similar if not identical to the 5800. } Some later instruments had greater Z travel than the 5800. The 5800 had } an archaic computer control system, whereas the 6000 series models had a } regular PC interface and hardware. (I think the 5900 may have had a PC } rather than the proprietary computer.) Our 5800 still works great, but I } am concerned about availability of parts as time goes on, especially for } the computer electronics. I am hopeful that we can keep our 5800 running } for a few more years, but we have had a couple of issues finding } components. } } We start installation of a new JEOL 6610 model next Monday. This } instrument has considerable improvements over previous models in the } 6000 series. The user interface is new with more imaging options and } better stage navigation. There is a new detector for VP mode that } provides imaging similar to secondary electron images. The signal to } noise on the BS detector is greatly improved and you can get great } backscatter images at very low KV. } } Hope this helps. } }
-- ~~~~~~~~~~~~~~~~~~~~ Bryan Bandli SEM Laboratory Manager University of Minnesota Duluth Life Science 93
Mail: UMD Geological Sciences 229 Heller Hall 1114 Kirby Dr. Duluth, MN 55812-3036
Phone: 218-726-7362 Fax: 218-726-8275
www.d.umn.edu/SEM/
==============================Original Headers============================== 14, 31 -- From bbandli-at-d.umn.edu Mon Dec 8 13:23:33 2008 14, 31 -- Received: from mx0.d.umn.edu (mx0.d.umn.edu [131.212.109.42]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mB8JNXsc011659 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:33 -0600 14, 31 -- Received: from mail.d.umn.edu (mail.d.umn.edu [131.212.109.2]) 14, 31 -- by mx0.d.umn.edu (8.13.8/8.13.8) with ESMTP id mB8JNXRk025521 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:33 -0600 (CST) 14, 31 -- Received: from mxv2.d.umn.edu (mxv2.d.umn.edu [131.212.109.136]) 14, 31 -- by mail.d.umn.edu (8.12.9/8.12.9) with ESMTP id mB8JNUPV002890 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:30 -0600 (CST) 14, 31 -- Received: from smtp.d.umn.edu (mx3.d.umn.edu [131.212.109.40]) 14, 31 -- by mxv2.d.umn.edu (8.13.8/8.13.8) with ESMTP id mB8JNUuw004913 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:30 -0600 (CST) 14, 31 -- Received: from [131.212.71.113] (nomad71-113.d.umn.edu [131.212.71.113]) 14, 31 -- (authenticated bits=0) 14, 31 -- by smtp.d.umn.edu (8.13.8/8.13.8) with ESMTP id mB8JMx5M011252 14, 31 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 14, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Dec 2008 13:23:04 -0600 (CST) 14, 31 -- Message-ID: {493D7414.7050300-at-d.umn.edu} 14, 31 -- Date: Mon, 08 Dec 2008 13:23:00 -0600 14, 31 -- From: Bryan Bandli {bbandli-at-d.umn.edu} 14, 31 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 14, 31 -- MIME-Version: 1.0 14, 31 -- To: Microscopy-at-microscopy.com 14, 31 -- Subject: Re: JEOL SEM's: comments on 5000 series vs 6000 14, 31 -- References: {200812072216.mB7MGnOv006169-at-ns.microscopy.com} 14, 31 -- In-Reply-To: {200812072216.mB7MGnOv006169-at-ns.microscopy.com} 14, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 31 -- Content-Transfer-Encoding: 7bit 14, 31 -- X-Virus-Scanned: ClamAV 0.94.2/8731/Sun Dec 7 22:31:15 2008 on mxv2.d.umn.edu 14, 31 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 26, 25 -- From kenconverse-at-qualityimages.biz Sat Dec 13 14:57:07 2008 26, 25 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 26, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBDKv7u8024220 26, 25 -- for {microscopy-at-microscopy.com} ; Sat, 13 Dec 2008 14:57:07 -0600 26, 25 -- Received: from Ken ([72.227.111.133]) by cdptpa-omta01.mail.rr.com 26, 25 -- with ESMTP 26, 25 -- id {20081213205706.OZWT18921.cdptpa-omta01.mail.rr.com-at-Ken} ; 26, 25 -- Sat, 13 Dec 2008 20:57:06 +0000 26, 25 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 26, 25 -- To: {bbandli-at-d.umn.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 26, 25 -- Subject: RE: [Microscopy] Re: JEOL SEM's: comments on 5000 series vs 6000 26, 25 -- Date: Sat, 13 Dec 2008 15:56:55 -0500 26, 25 -- Message-ID: {C2A966DAF744444AB6B055C3F7E1379C-at-Ken} 26, 25 -- MIME-Version: 1.0 26, 25 -- Content-Type: text/plain; 26, 25 -- charset="us-ascii" 26, 25 -- X-Priority: 3 (Normal) 26, 25 -- X-MSMail-Priority: Normal 26, 25 -- X-Mailer: Microsoft Outlook, Build 10.0.6838 26, 25 -- Importance: Normal 26, 25 -- Thread-Index: AclZartEncJM09C6TfeENTqw+gB1MwAIqjgg 26, 25 -- In-Reply-To: {200812081925.mB8JPWHs015923-at-ns.microscopy.com} 26, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 26, 25 -- Content-Transfer-Encoding: 8bit 26, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBDKv7u8024220 ==============================End of - Headers==============================
Can someone recommend a couple of anti FLAG antibodies that have produced published results of EM immunogold labeling? Thank you in advance and have a wonderful holiday season.
Hong Emory Univ. (404) 712-8491
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==============================Original Headers============================== 6, 32 -- From hyi-at-emory.edu Sun Dec 14 20:41:10 2008 6, 32 -- Received: from mr4.cc.emory.edu (mr4.cc.emory.edu [170.140.52.93]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBF2fAl3001772 6, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Dec 2008 20:41:10 -0600 6, 32 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 6, 32 -- by mr4.cc.emory.edu (8.13.1/8.13.1) with ESMTP id mBF2f54P025558 6, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Dec 2008 21:41:05 -0500 6, 32 -- Received: from EXCHHUB2.Enterprise.emory.net (170.140.30.54) by 6, 32 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server 6, 32 -- (TLS) id 8.1.336.0; Sun, 14 Dec 2008 21:41:15 -0500 6, 32 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 6, 32 -- EXCHHUB2.Enterprise.emory.net ([170.140.30.54]) with mapi; Sun, 14 Dec 2008 6, 32 -- 21:41:04 -0500 6, 32 -- From: "Yi, Hong" {hyi-at-emory.edu} 6, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 32 -- Date: Sun, 14 Dec 2008 21:38:08 -0500 6, 32 -- Thread-Topic: Anti FLAG antibodies 6, 32 -- Thread-Index: AQHJXl6Srsgzqf4v5UGktxPmGLiE0A== 6, 32 -- Message-ID: {3940C1839888A54DAE85AE20F294FB69010CBB9C542C-at-EXCHANGE12.Enterprise.emory.net} 6, 32 -- Accept-Language: en-US 6, 32 -- Content-Language: en-US 6, 32 -- X-MS-Has-Attach: 6, 32 -- X-MS-TNEF-Correlator: 6, 32 -- acceptlanguage: en-US 6, 32 -- Content-Type: text/plain; charset="us-ascii" 6, 32 -- MIME-Version: 1.0 6, 32 -- X-emory.edu-MailScanner: Found to be clean 6, 32 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu 6, 32 -- Subject: Re: Anti FLAG antibodies 6, 32 -- X-Spam-Status: No 6, 32 -- Content-Transfer-Encoding: 8bit 6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBF2fAl3001772 ==============================End of - Headers==============================
Can someone recommend an electron dense stain for polyacrylonitrile nano fibers for TEM morphology?
thank you
-- Fred A. Hayes Manager, Central Facilities Department of Chemical Engineering and Material Sciences 3118 Bainer Hall Bainer Hall Drive UC Davis Davis, CA 95616 530-752-0284 office 530-754-6350 fax 707-761-9045 cell fahayes-at-ucdavis.edu http://www.matscicf.ucdavis.edu/
==============================Original Headers============================== 11, 19 -- From fahayes-at-ucdavis.edu Mon Dec 15 13:56:57 2008 11, 19 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBFJuslQ016476 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 15 Dec 2008 13:56:56 -0600 11, 19 -- Received: from [169.237.230.156] ([169.237.230.156]) 11, 19 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with ESMTP id mBFJuqmK020135 11, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 15 Dec 2008 11:56:52 -0800 (PST) 11, 19 -- Message-ID: {4946B683.7020906-at-ucdavis.edu} 11, 19 -- Date: Mon, 15 Dec 2008 11:56:51 -0800 11, 19 -- From: Fred Hayes {fahayes-at-ucdavis.edu} 11, 19 -- User-Agent: Thunderbird 2.0.0.17 (Windows/20080914) 11, 19 -- MIME-Version: 1.0 11, 19 -- To: Microscopy-at-Microscopy.Com 11, 19 -- Subject: staining polyacrylonitrile fibers 11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- X-Virus-Scanned: ClamAV version 0.93.3, clamav-milter version 0.93.3 on av4 11, 19 -- X-Virus-Status: Clean 11, 19 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nabil.bassim-at-nrl.navy.mil as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] 2nd Annual Washington DC Focused Ion Beam User Group Meeting/Workshop - Feb 23, 2009
Question: Announcing the
2nd Annual Washington DC Focused Ion Beam User Group Meeting/Workshop
February 23rd , 2009
Location: Friedman Room, Building 226, U.S. Naval Research Laboratory, Washington DC
*Co-sponsored by MAMAS (Mid-Atlantic Microbeam Analysis Society)
In February 2008, the Washington DC Area Focused Ion Beam User Group assembled for the first time to discuss the idea of getting together to discuss the latest work in the DC Area using Focused Ion Beams on a regular basis. We had a very enthusiastic opening response! As a result, we are inviting you to the 2nd Annual Washington DC Focused Ion Beam User Group Meeting/Workshop. During the day, we will have presentations by FIB users discussing interesting new FIB applications and a tour of the NRL Nanoscience Institute. There will also be opportunities for informal discussion of new techniques and applications as well as the opportunity to share /tricks of the trade/.
If you would like to attend the workshop, please contact Nabil Bassim (nabil.bassim-at-nrl.navy.mil), Keana Scott (keana.scott-at-nist.gov), or Ken Livi (klivi-at-jhu.edu) by January 26th , 2008. If you would like to contribute a talk about the latest FIB applications or recent research in your laboratory using FIB, please send us a title for the talk by *January 26^th , 2009*. Please let us know if you are a foreign national so that we can arrange for security passes at NRL. Also, please forward this message to anyone in the DC area who may be interested and we may have missed.
We look forward to seeing you at NRL this February!!
Nabil Bassim (Naval Research Laboratory)
Keana Scott (National Institute of Standards and Technology)
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Email: cindy.smith-at-nephropath.com Name: Cindy Hastings Smith
Organization: Nephropath
Title-Subject: [Filtered] Schematics for Coolwell Chiller
Question: We are in DESPERATE need of the schematics for one of our Coolwell chillers! We have a Zeiss EM109 (that we happily acquired from Debby Sherman!) and the chiller is having problems--we are blowing fuses and burning up relays. Please Help if you can...You may contact me off-line by email or phone.
Thanks in advance-- Cindy Hastings Smith Nephropath 501-604-2695 cindy.smith-at-nephropath.com
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Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: University of Saskatchewan
Title-Subject: [Filtered] No beam for Philips SEM 505
Question: Hello,
I got a problem with our "old" Philips SEM 505: after pressing HT button, the emission current reached at the maximum reading (} 200) and kept there forever; and no beam was available. I changed the filament but no help.
Any clues or suggestions would be greatly appreciated!
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Email: robert.clark-at-sharp.com Name: Robert Clark
Organization: Sharp HealthCare
Title-Subject: [Filtered] Staining thick sections for Congo Red (amyloid)
Question: Dear all,
I am wondering if anyone has performed a Congo Red Stain for amyloid on thick sections from Eponate12 embedded cells; and if so, would they kindly share their protocol?
The sample was originally a cell block embedded in paraffin (vitreous fluid), and Iíve since deparaffinized it and used this material for EM studies which shows possible amyloid fibrils. There is no remaining tissue left in paraffin so the resin embedded cells are my only option.
I know there is a way to perform an H&E stain from resin but I am not sure about using unstained sections for special stains. Any knowledge shared would be greatly appreciated.
Best regards,
Robert
_________________________ Robert C Clark Electron Microscopist Sharp HealthCare San Diego, CA Lab-619.295.0824 Mobile-858.245.2892
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Organization: GATAN, INC.
Title-Subject: [Filtered] Magnesium TEM Prep
Question: Hello Listers,
I have a colleague interested in preparing Magnesium powder for TEM analysis. What methods, other than electropolishing, are safe to use without altering the low-density metal.
Is the column and apertures aligned? How about filament alignment?
One large diameter aperture helps get a beam and then center it and work down to smaller apertures for final alignment. Is this the first time you've had this problem?
gary g.
At 05:54 PM 12/15/2008, you wrote:
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==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Mon Dec 15 20:07:17 2008 9, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBG27HSB016813 9, 20 -- for {microscopy-at-microscopy.com} ; Mon, 15 Dec 2008 20:07:17 -0600 9, 20 -- Message-Id: {200812160207.mBG27HSB016813-at-ns.microscopy.com} 9, 20 -- Received: (qmail 18654 invoked from network); 15 Dec 2008 18:05:03 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 18651, pid: 18652, t: 0.1055s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by smtp2 with SMTP; 15 Dec 2008 18:05:03 -0800 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Mon, 15 Dec 2008 18:07:13 -0800 9, 20 -- To: gul417-at-mail.usask.ca 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: No beam for Philips SEM 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200812160154.mBG1ssxI032037-at-ns.microscopy.com} 9, 20 -- References: {200812160154.mBG1ssxI032037-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I'm not familiar with the Coolwell but Zeiss tends to use only (or favorably) turbine chillers. With these, the pump runs all the time and the turbine eliminates cavitation interference. Does the chiller have a hot gas bypass? Zeiss generally does not want the chiller to short cycle. The HGB will keep the compressor running and maintain a rather constant tank water temperature.
Short cycling can burn out the start current relay on the compressor.
Also check that you are running the right flow rate and all filters are clean. Check hoses for crud.
Schematics for a chiller are not all that complicated.
gary g.
At 05:54 PM 12/15/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Mon Dec 15 20:13:15 2008 11, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBG2DEaW002239 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 15 Dec 2008 20:13:14 -0600 11, 20 -- Message-Id: {200812160213.mBG2DEaW002239-at-ns.microscopy.com} 11, 20 -- Received: (qmail 20642 invoked from network); 15 Dec 2008 18:11:01 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 20639, pid: 20640, t: 0.1040s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by smtp2 with SMTP; 15 Dec 2008 18:11:01 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Mon, 15 Dec 2008 18:13:10 -0800 11, 20 -- To: cindy.smith-at-nephropath.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] viaWWW: Schematics for Coolwell Chiller 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200812160154.mBG1sPAO029984-at-ns.microscopy.com} 11, 20 -- References: {200812160154.mBG1sPAO029984-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
- set HT to lowest value, emission to lowest value (both on control panel and on the gun), see if emission current goes down;
- remove wehnelt assembly, close gun, pump down, turn ON the HT - if emission current stays near zero, then filament was touching the cup, but if not -
- remove HT cable from HT generator unit (under the SEM, on the floor) and turn ON the HT- if emission current stays near zero, then problem is likely in the gun connections/circuitry/cable, but if current still maxed out, then problem is most likely in HT generator unit. Check fuses in it, just in case, but I doubt fuse will do this.
Component level troubleshooting may be still required no matter what. These symptoms could be caused by faulty HT/gun control circuits and power supply circuits - not necessarily by HT generator and emission units.
We have complete set of all units and assemblies for this SEM, just in case.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {gul417-at-mail.usask.ca} To: {vitalylazar-at-att.net} Sent: Monday, December 15, 2008 8:54 PM
I guess why bother commenting on a specific SEM problem if I don't have that SEM?
No matter, they are all complex tools. The fixes for problems for different SEMs are indeed different.
I am glad that you are there to point this out.
gary g.
At 07:04 PM 12/15/2008, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Mon Dec 15 22:47:57 2008 10, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBG4lvtR004198 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 15 Dec 2008 22:47:57 -0600 10, 20 -- Message-Id: {200812160447.mBG4lvtR004198-at-ns.microscopy.com} 10, 20 -- Received: (qmail 7333 invoked from network); 15 Dec 2008 20:47:36 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 7330, pid: 7331, t: 0.1460s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 10, 20 -- by smtp1 with SMTP; 15 Dec 2008 20:47:36 -0800 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 20 -- Date: Mon, 15 Dec 2008 20:47:54 -0800 10, 20 -- To: vitalylazar-at-att.net 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] Re: viaWWW: No beam for Philips SEM 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200812160304.mBG34dGf002060-at-ns.microscopy.com} 10, 20 -- References: {200812160304.mBG34dGf002060-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I scrapped a JEOL JEM 100CX TEM this summer and although not the same machine I suspect it is still the same technology. My microscope was from 1979 and had oil diffusion pumps. In it I found transformer oil in the high tension unit and the current regulators for the lenses. Two mercury batteries were used as voltage references. Then there was the oil in the pumps and there was a small lead cover over a port in the column.
After that point it was more or less cables, steel, aluminium, brass, copper and electronics scrap. I sorted it all and sold most of it as scrap. I kept the circuit boards, pumps, valves and other components as spare parts for my other TEM and to sell if I could find a buyer.
Regards, Göran
gary-at-gaugler.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } If you get any definitive or procedural info on this } subject in general, I for one would really appreciate } knowing about it and the details. } } I think this is a very tricky subject based on the } technology foundation of the SEM at its time of manufacture. } Certainly there are other variables. But either way, this } could be a big problem for someone wanting to dispose of a } SEM or a TEM. } } gary g. } } } } } At 01:42 PM 12/12/2008, you wrote: } } } } } } Email: emer.ryan-at-dit.ie } } Name: Emer Ryan } } } } Organization: CREST DIT Dublin } } } } Title-Subject: [Filtered] Info for disposal of JEOL T200 SEM } } } } Question: Hello listers. } } } } I am about to dispose of a JEOL T200 SEM. In order to do so, I need } } to know the quantities of heavy metals and any other hazardous } } substances etc associated with it. The waste disposal folk are } } requesting MSDS for same. As long as I have been in this institute, } } this instrument has sat in a corner, unused. There is no manual, } } nothing etc for it. I will endeavour to get the required information } } } } from JEOL, of course, but if anyone has any advice or information, } } } I'd be grateful. } } Regards } } Emer. } } } } } } Login Host: 147.252.66.48 } }
==============================Original Headers============================== 7, 24 -- From axelsson-at-acc.umu.se Tue Dec 16 03:15:45 2008 7, 24 -- Received: from mail.acc.umu.se (mail.acc.umu.se [130.239.18.156]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBG9FiNO012681 7, 24 -- for {microscopy-at-microscopy.com} ; Tue, 16 Dec 2008 03:15:45 -0600 7, 24 -- Received: from localhost (localhost [127.0.0.1]) 7, 24 -- by amavisd-new (Postfix) with ESMTP id C299E44; 7, 24 -- Tue, 16 Dec 2008 10:15:43 +0100 (MET) 7, 24 -- X-Virus-Scanned: amavisd-new at acc.umu.se 7, 24 -- Received: from [192.168.1.4] (1-1-10-48a.um.um.bostream.se [82.182.239.55]) 7, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 24 -- (No client certificate requested) 7, 24 -- by mail.acc.umu.se (Postfix) with ESMTPS id 1857C43; 7, 24 -- Tue, 16 Dec 2008 10:15:40 +0100 (MET) 7, 24 -- Message-ID: {494771BC.8090403-at-acc.umu.se} 7, 24 -- Date: Tue, 16 Dec 2008 10:15:40 +0100 7, 24 -- From: =?ISO-8859-1?Q?G=F6ran_Axelsson?= {axelsson-at-acc.umu.se} 7, 24 -- User-Agent: Thunderbird 2.0.0.18 (X11/20081125) 7, 24 -- MIME-Version: 1.0 7, 24 -- To: gary-at-gaugler.com, emer.ryan-at-dit.ie, microscopy-at-microscopy.com 7, 24 -- Subject: Re: [Microscopy] Re: viaWWW: Info for disposal of JEOL T200 SEM 7, 24 -- References: {200812130723.mBD7NFHc003069-at-ns.microscopy.com} 7, 24 -- In-Reply-To: {200812130723.mBD7NFHc003069-at-ns.microscopy.com} 7, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have a client that has successfully used an acrolein fixative for difficult fungal specimens. His supply of sealed 10 ml ampoules of acrolein diluted in 0.1 M cacodylate has nearly run out. It seems our usual favorite EM chemicals suppliers no longer sell it.
Sigma sells the pure stuff } 99% in 10 ml ampoules. They cost about $50 each, plus shipping is almost TWICE that. As we only need to use small volumes of fix at a time, we would like to dilute the 10 ml of pure acrolein to 3% in buffer, divide it into small aliquots, and store it somehow so it remain stable and viable for months to a few years.
The question is how to store it. We think storage at -20 C, in glass vials with screw caps, stored inside cans with tight lids (like osmium solutions are shipped in) might be a good way to go.
We would like to hear your ideas on storage of dilute, buffered acrolein, and how long we could expect it to remain viable as a fixative.
We are quite aware of how nasty acrolein is. Any advice you can give us on safe storage for the long term will be appreciated.
Thanks in advance,
Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
==============================Original Headers============================== 8, 20 -- From ahlst007-at-umn.edu Wed Dec 17 11:07:02 2008 8, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBHH72DG030817 8, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 17 Dec 2008 11:07:02 -0600 8, 20 -- Received: from x-160-94-173-219.cbs.umn.edu (x-160-94-173-219.cbs.umn.edu [160.94.173.219]) 8, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP 8, 20 -- Wed, 17 Dec 2008 11:07:02 -0600 (CST) 8, 20 -- X-Umn-Remote-Mta: [N] x-160-94-173-219.cbs.umn.edu [160.94.173.219] #+LO+TS+AU 8, 20 -- X-Umn-Classification: local 8, 20 -- Message-ID: {494930AE.608-at-umn.edu} 8, 20 -- Date: Wed, 17 Dec 2008 11:02:38 -0600 8, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 8, 20 -- Reply-To: ahlst007-at-umn.edu 8, 20 -- Organization: Imaging Center UM 8, 20 -- User-Agent: Thunderbird 2.0.0.18 (Macintosh/20081105) 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Microscopy-at-Microscopy.com 8, 20 -- Subject: Acrolein fixative storage 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Can't you buy unsealed, empty ampoules that can be flamed to close? I would think this is essential for a volatile substance like acrolein. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: ahlst007-at-umn.edu [mailto:ahlst007-at-umn.edu] Sent: Wednesday, December 17, 2008 11:08 AM To: Phillips, Thomas E.
Dear Listers,
I have a client that has successfully used an acrolein fixative for difficult fungal specimens. His supply of sealed 10 ml ampoules of acrolein diluted in 0.1 M cacodylate has nearly run out. It seems our usual favorite EM chemicals suppliers no longer sell it.
Sigma sells the pure stuff } 99% in 10 ml ampoules. They cost about $50 each, plus shipping is almost TWICE that. As we only need to use small volumes of fix at a time, we would like to dilute the 10 ml of pure acrolein to 3% in buffer, divide it into small aliquots, and store it somehow so it remain stable and viable for months to a few years.
The question is how to store it. We think storage at -20 C, in glass vials with screw caps, stored inside cans with tight lids (like osmium solutions are shipped in) might be a good way to go.
We would like to hear your ideas on storage of dilute, buffered acrolein, and how long we could expect it to remain viable as a fixative.
We are quite aware of how nasty acrolein is. Any advice you can give us on safe storage for the long term will be appreciated.
Thanks in advance,
Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
==============================Original Headers============================== 8, 20 -- From ahlst007-at-umn.edu Wed Dec 17 11:07:02 2008 8, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBHH72DG030817 8, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 17 Dec 2008 11:07:02 -0600 8, 20 -- Received: from x-160-94-173-219.cbs.umn.edu (x-160-94-173-219.cbs.umn.edu [160.94.173.219]) 8, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP 8, 20 -- Wed, 17 Dec 2008 11:07:02 -0600 (CST) 8, 20 -- X-Umn-Remote-Mta: [N] x-160-94-173-219.cbs.umn.edu [160.94.173.219] #+LO+TS+AU 8, 20 -- X-Umn-Classification: local 8, 20 -- Message-ID: {494930AE.608-at-umn.edu} 8, 20 -- Date: Wed, 17 Dec 2008 11:02:38 -0600 8, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 8, 20 -- Reply-To: ahlst007-at-umn.edu 8, 20 -- Organization: Imaging Center UM 8, 20 -- User-Agent: Thunderbird 2.0.0.18 (Macintosh/20081105) 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Microscopy-at-Microscopy.com 8, 20 -- Subject: Acrolein fixative storage 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 29 -- From PhillipsT-at-missouri.edu Wed Dec 17 11:17:49 2008 21, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBHHHniU010858 21, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Dec 2008 11:17:49 -0600 21, 29 -- X-IronPort-Anti-Spam-Filtered: true 21, 29 -- X-IronPort-Anti-Spam-Result: AsAEANvCSEnRauUo/2dsb2JhbACSH6xIWIR6DQEJhnKFMAGBe4EK 21, 29 -- Received: from unknown (HELO um-tsmtpout1.um.umsystem.edu) ([209.106.229.40]) 21, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 17 Dec 2008 11:17:48 -0600 21, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 21, 29 -- Wed, 17 Dec 2008 11:17:48 -0600 21, 29 -- x-mimeole: Produced By Microsoft Exchange V6.5 21, 29 -- Content-class: urn:content-classes:message 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Type: text/plain; 21, 29 -- charset="us-ascii" 21, 29 -- Subject: RE: [Microscopy] Acrolein fixative storage 21, 29 -- Date: Wed, 17 Dec 2008 11:17:27 -0600 21, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05902AD9-at-UM-XMAIL06.um.umsystem.edu} 21, 29 -- In-Reply-To: {200812171708.mBHH8AGk031981-at-ns.microscopy.com} 21, 29 -- X-MS-Has-Attach: 21, 29 -- X-MS-TNEF-Correlator: 21, 29 -- Thread-Topic: [Microscopy] Acrolein fixative storage 21, 29 -- Thread-Index: Aclgagpdfh6Zqq1RRJSSVg5c9CvrxQAASv9g 21, 29 -- References: {200812171708.mBHH8AGk031981-at-ns.microscopy.com} 21, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 21, 29 -- To: {Microscopy-at-microscopy.com} 21, 29 -- X-OriginalArrivalTime: 17 Dec 2008 17:17:48.0717 (UTC) FILETIME=[62812DD0:01C9606B] 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBHHHniU010858 ==============================End of - Headers==============================
Have you asked the EM Suppliers if they could make a special order for you? I would think that this would be the way to go. Over the years I remember both Electron Microscopy Sciences and SPI have advertised that they can get or make things on request and I suspect that others have also made this statement.
You do not want to handle the pure chemical if possible. The last I had bought was from Kodak around 1973 and I know it works great mixed into a fixative for difficult samples. I remember it well for the 100 ml bottle tipped over when I was recapping it, spilling a bit onto my lab coat. Later, after not being able to breathe for what seemed to be a very long while (literally), I learned that diluted acrolein is what gives humans problems when exposed to tear gas.
Pat
Patricia Stranen Connelly Biologist NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
} Dear Listers, } } I have a client that has successfully used an } acrolein fixative for difficult fungal } specimens. His supply of sealed 10 ml ampoules } of acrolein diluted in 0.1 M cacodylate has } nearly run out. It seems our usual favorite EM } chemicals suppliers no longer sell it. } } Sigma sells the pure stuff } 99% in 10 ml } ampoules. They cost about $50 each, plus } shipping is almost TWICE that. As we only need } to use small volumes of fix at a time, we would } like to dilute the 10 ml of pure acrolein to 3% } in buffer, divide it into small aliquots, and } store it somehow so it remain stable and viable } for months to a few years. } } The question is how to store it. We think } storage at -20 C, in glass vials with screw } caps, stored inside cans with tight lids (like } osmium solutions are shipped in) might be a good } way to go. } } We would like to hear your ideas on storage of } dilute, buffered acrolein, and how long we could } expect it to remain viable as a fixative. } } We are quite aware of how nasty acrolein is. Any } advice you can give us on safe storage for the } long term will be appreciated. } } Thanks in advance, } } Gib } -- } Gilbert (Gib) Ahlstrand, Electron Microscopist, } Imaging Center, University of Minnesota } 123 Snyder Hall } St. Paul, MN 55108 } http://www.cbs.umn.edu/ic
==============================Original Headers============================== 11, 27 -- From connellyps-at-nhlbi.nih.gov Wed Dec 17 11:53:05 2008 11, 27 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBHHr5DQ026692 11, 27 -- for {microscopy-at-microscopy.com} ; Wed, 17 Dec 2008 11:53:05 -0600 11, 27 -- X-IronPortListener: Outbound_SMTP 11, 27 -- Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) 11, 27 -- by nihxway2out.hub.nih.gov with ESMTP; 17 Dec 2008 12:50:01 -0500 11, 27 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); 11, 27 -- Wed, 17 Dec 2008 12:49:56 -0500 11, 27 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.163]) with Microsoft Exchange Server HTTP-DAV ; 11, 27 -- Wed, 17 Dec 2008 17:49:31 +0000 11, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 11, 27 -- Date: Wed, 17 Dec 2008 12:48:56 -0500 11, 27 -- Subject: Re: [Microscopy] Acrolein fixative storage 11, 27 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 11, 27 -- To: {ahlst007-at-umn.edu} , 11, 27 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 27 -- Message-ID: {C56EA5B8.2BA0%connellyps-at-nhlbi.nih.gov} 11, 27 -- Thread-Topic: [Microscopy] Acrolein fixative storage 11, 27 -- Thread-Index: Aclgb7t9+d+uC8xiEd2y2wANk2Yv1A== 11, 27 -- In-Reply-To: {200812171713.mBHHDfFx006067-at-ns.microscopy.com} 11, 27 -- Mime-version: 1.0 11, 27 -- Content-type: text/plain; 11, 27 -- charset="ISO-8859-1" 11, 27 -- X-OriginalArrivalTime: 17 Dec 2008 17:49:56.0929 (UTC) FILETIME=[DFCEFB10:01C9606F] 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBHHr5DQ026692 ==============================End of - Headers==============================
I have a researcher who will be wanting immunoEM on cell pellets. I'm going to embed them into LR White.
What I want to know is what is the best type of gold to buy and from who? The researcher isn't sure what the source of the primary will be (rabbit, mouse, Sasquatch) so I was wondering if Protein A would be the best all around choice.
I haven't done immunoEM in a while so I was wondering what's the latest and best in terms of optimizing the staining. Remember I have to go with a room temp embedding media, I have no acccess to low temp equipment.
Thanks in advance.
Does Rudolph's nose turn black when he has a cold?
Paula :-)
Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397
==============================Original Headers============================== 11, 25 -- From vapatpxs-at-yahoo.com Wed Dec 17 12:05:19 2008 11, 25 -- Received: from n26.bullet.mail.mud.yahoo.com (n26.bullet.mail.mud.yahoo.com [68.142.206.221]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBHI5JpH008298 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 17 Dec 2008 12:05:19 -0600 11, 25 -- Received: from [68.142.194.244] by n26.bullet.mail.mud.yahoo.com with NNFMP; 17 Dec 2008 18:05:19 -0000 11, 25 -- Received: from [68.142.201.71] by t2.bullet.mud.yahoo.com with NNFMP; 17 Dec 2008 18:05:19 -0000 11, 25 -- Received: from [127.0.0.1] by omp423.mail.mud.yahoo.com with NNFMP; 17 Dec 2008 18:05:19 -0000 11, 25 -- X-Yahoo-Newman-Property: ymail-3 11, 25 -- X-Yahoo-Newman-Id: 82318.50856.bm-at-omp423.mail.mud.yahoo.com 11, 25 -- Received: (qmail 89581 invoked by uid 60001); 17 Dec 2008 18:05:18 -0000 11, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 25 -- s=s1024; d=yahoo.com; 11, 25 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 11, 25 -- b=Z85KKvKy3uEhSCNTbRCH8v+vWanCWewUnXzsg01JkQsaEepc9x5tCwS+h6dORwLinDjis9XHUTeYu5gZYo2OEqnoPHaHsMHxzrsMCzbs3m8FqiQ03l7/OV+Qt/Utif5pa6nGxVe23hg61afd3+QMtWZA0mHATcHuXFjT+EjWuHs=; 11, 25 -- X-YMail-OSG: eaejRV0VM1m3P51m4LYeiUah441wHmrq.BhcflkuLIBGEW1wAqM4Ir7cjz8o4BqlKuUv0Wi54n01HoscZbsAVC8p0Oj3bxOcTVjo0gR_yO_aOQfvP1YsIMa.ANeSA9C4gqYJgcgbE5f91m4hQSretwucvOU- 11, 25 -- Received: from [132.239.85.200] by web46109.mail.sp1.yahoo.com via HTTP; Wed, 17 Dec 2008 10:05:17 PST 11, 25 -- X-Mailer: YahooMailWebService/0.7.260.1 11, 25 -- Date: Wed, 17 Dec 2008 10:05:17 -0800 (PST) 11, 25 -- From: Va Paula Sicurello {vapatpxs-at-yahoo.com} 11, 25 -- Reply-To: vapatpxs-at-yahoo.com 11, 25 -- Subject: LR White and gold 11, 25 -- To: MSA BB {Microscopy-at-microscopy.com} 11, 25 -- MIME-Version: 1.0 11, 25 -- Content-Type: text/plain; charset=us-ascii 11, 25 -- Message-ID: {27601.88904.qm-at-web46109.mail.sp1.yahoo.com} ==============================End of - Headers==============================
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Email: dagmorga-at-indiana.edu Name: David Morgan
Organization: Indiana University
Title-Subject: [Filtered] carbon evaporator
Question: Hi,
Someone here is planning to purchase a Denton Desk IV carbon evaporator to use with a new SEM that is being installed in a few days. They are looking a the version of the system that does NOT have a turbo molecular pump and that is marketed as producing a larger grain size than the turbo pumped instrument. Does anyone have experience with using the Desk IV to make carbon foils for EM grids? I suspect that it doesn't work (or doesn't work well) for this purpose, but I'd like to hear from anyone with practical expeience.
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Question: I am just wondering if sombody can advice me the company/organization that could make a custom stage for my digital microscope. I would like to use this microscope to inspect the mill roll microstructure on the plant at high (1000x) magnification. The standard microscope stage has a flat base and is unstable on the roll. I am thinking about tripot type stand with magneting fastening. Any advice or tip? Thank you in advance Zofia
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both srijay-at-stanford.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: srijay-at-stanford.edu Name: Jay
Organization: Stanford University
Title-Subject: [Filtered] removal of glass coverslips
Question: Hi:
Can anybody tell me how to remove glass coverslips from the resin. I have tried plunging it in liquid nitrogen or putting it in boiling water and then immersing in liquid nitrogen and it does not work. I am not comfortable using hydroflouric acid - any other suggestions would be greatly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both allan.mitchell-at-stonebow.otago.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: allan.mitchell-at-stonebow.otago.ac.nz Name: Allan Mitchell
Organization: University of otago
Title-Subject: [Filtered] Human ITO cells
Question: Hi all
I have been asked by one of the teaching staff of our Anatomy Department if I have a good transmission electron microscopy image of an ITO cell, preferably from human. This image is to be used in a teaching handout currently being produced.
I do not have any such images, or human liver specimen blocks, available in my archive. Normal human liver is difficult to get.
Does anyone have TEM images of a normal hepatic stellate cells (preferably human) that they would be willing to be allowed to be used in this way, or know of someone who may .
The teacher I am working with will credit the source and is willing to pay a small fee for a suitable image.
Have a great christmas.
Allan
Allan Mitchell Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ Department: http://anatomy.otago.ac.nz/
You really should know the source of your primary in order to decide which gold conjugate to use. While Protein A-gold is my, and many others', favorite - because it gives closer detection and only one gold (or less ;) ) will bind to each primary - Protein A only binds well to IgGs from rabbit, pig, guinea pig (as well as some from human). So no, it is not an all-around choice. You could, though, use a bridging antibody - e.g., a rabbit-anti-mouse between that mouse monoclonal and Protein A. In this case, PAG will be indeed your universal probe, but you'll need to buy other Abs to bridge.
Not meaning to hijack, but I would be really interested if somebody could comment here on which *primary* is be preferable in those cases when there is a choice? Or, should I say, *which polyclonal*, since we know polyclonal works better for immunoEM? Rabbit, guinea pig?.. Or, perhaps those IgYs from chicken - won't bind Protein A, but I remember hearing somewhere that chicken Abs tend to give "cleaner" labeling, while those from rabbit are among the "dirtiest"? Anybody?..
As for source, I don't think you can go wrong with any. I used conjugates bought from EMS/Aurion, as well as from Ted Pella/BBI, Jackson ImmunoResearch, and even some from Sigma - no problem ever with gold probe. Wanted to blame it on gold a few times, but always turned out to be my fault in the end :)
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and not his employer. These opinions and experiences do not represent a consensus of the NIH scientific community. On the good side, this message is not confidential and can be freely shared and reproduced.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Listers, } } I have a researcher who will be wanting immunoEM on cell pellets. } I'm going to embed them into LR White. } } What I want to know is what is the best type of gold to buy and from } who? The researcher isn't sure what the source of the primary will } be (rabbit, mouse, Sasquatch) so I was wondering if Protein A would } be the best all around choice. } } I haven't done immunoEM in a while so I was wondering what's the } latest and best in terms of optimizing the staining. Remember I } have to go with a room temp embedding media, I have no acccess to } low temp equipment. } } Thanks in advance. } } Does Rudolph's nose turn black when he has a cold? } } Paula :-) } } Paula Sicurello } VA Medical Center San Diego } Veterans Medical Research Foundation (VMRF) } Core Microscope Facility, room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } } } } ==============================Original } Headers============================== } 11, 25 -- From vapatpxs-at-yahoo.com Wed Dec 17 12:05:19 2008 } 11, 25 -- Received: from n26.bullet.mail.mud.yahoo.com } (n26.bullet.mail.mud.yahoo.com [68.142.206.221]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amcgroup2-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: amcgroup2-at-aol.com Name: Dave Sanders
Organization: AMC Group
Title-Subject: [Filtered] FIB/SEM/TEM Prep technician position
Question: AMC Group (Mesa, AZ) currently has an immediate opening for a highly skilled FIB operator (contractor) to prepare specimens for SEM and TEM analysis. 2-5 years of related experience is required and flexibility to work in various shifts is expected.
If qualified and interested, please email your resume and list of references directly to my attention.
Thanks,
David Sanders, Ph.D. AMC Group AMCGroup2-at-aol.com
Why would an organization seek support staff and have an aol TLD? Can you explain this? In history, this is a joke. I'm not applying this to you but it sounds like you are a start-up. If so, that might be useful to disclose to potential employees. Defining the FIB brand and model would also help. Is there a maintenance contract on the FIB?
I think that you should better define what a contractor position is relative to this job, and also, what highly skilled means. Does working on multiple shifts quality for this variable?
I think that you need a better job summary. Others have done a good job doing this. It ought to help you and the applicants.
No...I am not applying for this position. Your HR people ought to have done a better job than what is seen here. That is IMO.
gary g.
At 08:22 PM 12/17/2008, you wrote:
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==============================Original Headers============================== 12, 20 -- From gary-at-gaugler.com Wed Dec 17 22:55:10 2008 12, 20 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 12, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBI4t9Rj020363 12, 20 -- for {microscopy-at-microscopy.com} ; Wed, 17 Dec 2008 22:55:09 -0600 12, 20 -- Message-Id: {200812180455.mBI4t9Rj020363-at-ns.microscopy.com} 12, 20 -- Received: (qmail 10520 invoked from network); 17 Dec 2008 20:51:50 -0800 12, 20 -- Received: by simscan 1.1.0 ppid: 10517, pid: 10518, t: 0.2744s 12, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 12, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 12, 20 -- by smtp2 with SMTP; 17 Dec 2008 20:51:50 -0800 12, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 12, 20 -- Date: Wed, 17 Dec 2008 20:54:06 -0800 12, 20 -- To: amcgroup2-at-aol.com 12, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 12, 20 -- Subject: Re: [Microscopy] viaWWW: FIB/SEM/TEM Prep technician position 12, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 12, 20 -- In-Reply-To: {200812180422.mBI4MO42008731-at-ns.microscopy.com} 12, 20 -- References: {200812180422.mBI4MO42008731-at-ns.microscopy.com} 12, 20 -- Mime-Version: 1.0 12, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I do not have experience with the type of coater that you mention but some work I carried out with Edwards High Vacuum company many many years ago may explain the situation?
We experimented with vacuum level and its relationship to carbon film quality when examined in a TEM. Very simply the better the vacuum the better the coating. We used carbon rods and carbon string and in both cases it was the vacuum that was critical, not the type of coater.
I am sure some of the regular vacuum experts will back this finding?
Whilst on the subject the greatest problem that people face when evaporating carbon is the rod type. Even purchasing from you original supplier you may not receive exactly the type of rod you purchased several years earlier. This means you will need to calibrate the system all over again in order to optimise evaporation. My tip is if you have a system working well, with a recently purchased rod package, immediately order some more! It is the experience of many of my customers that waiting years for your current supply to be used up almost certainly means starting all over again with your new supply!
Happy coating and Seasons Greetings
Steve Chapman Protrain For training and consultancy in electron microscopy world wide Tel +44 1280 816512 Fax +44 1280 814007 Cell +44 7711 606967 www.emcourses.com
-----Original Message----- X-from: dagmorga-at-indiana.edu [mailto:dagmorga-at-indiana.edu] Sent: 17 December 2008 23:01 To: protrain-at-emcourses.com
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Email: dagmorga-at-indiana.edu Name: David Morgan
Organization: Indiana University
Title-Subject: [Filtered] carbon evaporator
Question: Hi,
Someone here is planning to purchase a Denton Desk IV carbon evaporator to use with a new SEM that is being installed in a few days. They are looking a the version of the system that does NOT have a turbo molecular pump and that is marketed as producing a larger grain size than the turbo pumped instrument. Does anyone have experience with using the Desk IV to make carbon foils for EM grids? I suspect that it doesn't work (or doesn't work well) for this purpose, but I'd like to hear from anyone with practical expeience.
Hello Zofia, I'd try making a replica of the surface and examining it back in the lab. I heard Dale Quakenbush make a presentation on using plastic coverslips and a drop of solvent to replicate paper mill rollers. I would try a plastic cover slip and a little heat and pressure to replicate the surface. After it cools, peal the plastic slip off and you should have a nice replica you can examine back in the lab.
As a side note, a 1000x magnification with the light scope strongly implies oil immersion, if you want the resolution to see the fine detail available at 1000X. Remember, the equation for resolution involves NA of the objective, wavelength of light and the lowest refractive index in the sample/optic configuration. Air is 1 oil is around 1.5. Me, I'd try the SEM first.
Stay safe.. Frank Karl.
zofia.niemczura-at-a rcelormittal.com To 12/17/2008 06:13 frank_karl-at-lincolnelectric.com PM cc
Subject Please respond to [Microscopy] viaWWW: microscope zofia.niemczura-at-a stand rcelormittal.com
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Question: I am just wondering if sombody can advice me the company/organization that could make a custom stage for my digital microscope. I would like to use this microscope to inspect the mill roll microstructure on the plant at high (1000x) magnification. The standard microscope stage has a flat base and is unstable on the roll. I am thinking about tripot type stand with magneting fastening. Any advice or tip? Thank you in advance Zofia
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Wed Dec 17 16:59:57 2008 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBHMxuLZ029939 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 17 Dec 2008 16:59:57 -0600 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240802c56f34cc31ae-at-[206.69.208.22]} 6, 11 -- Date: Wed, 17 Dec 2008 16:59:55 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: zofia.niemczura-at-arcelormittal.com (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: microscope stand 6, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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==============================Original Headers============================== 25, 22 -- From frank_karl-at-lincolnelectric.com Thu Dec 18 06:14:42 2008 25, 22 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 25, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBICEgYH025875 25, 22 -- for {microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 06:14:42 -0600 25, 22 -- In-Reply-To: {200812172313.mBHND37r015137-at-ns.microscopy.com} 25, 22 -- Subject: Re: [Microscopy] viaWWW: microscope stand 25, 22 -- To: zofia.niemczura-at-arcelormittal.com, Microscopy-at-microscopy.com 25, 22 -- X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 25, 22 -- Message-ID: {OF282E5069.F229CF4D-ON85257523.00423C00-85257523.00432AC9-at-lincolnelectric.com} 25, 22 -- Date: Thu, 18 Dec 2008 07:14:29 -0500 25, 22 -- From: Frank_Karl-at-lincolnelectric.com 25, 22 -- X-MIMETrack: CD-MIME by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 25, 22 -- 07, 2008) at 12/18/2008 07:14:29 AM, 25, 22 -- CD-MIME complete at 12/18/2008 07:14:29 AM, 25, 22 -- Itemize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 25, 22 -- 07, 2008) at 12/18/2008 07:14:29 AM, 25, 22 -- Serialize by Router on Notescom1/Lincoln Electric/US(Release 8.0.1|February 25, 22 -- 07, 2008) at 12/18/2008 07:14:29 AM, 25, 22 -- Serialize complete at 12/18/2008 07:14:29 AM 25, 22 -- MIME-Version: 1.0 25, 22 -- Content-Type: text/plain; 25, 22 -- charset="US-ASCII" ==============================End of - Headers==============================
Are we talking about a sputter coater here? If this is the case, I can tell you that I already tried to cover formvar grids with carbon using a cressington instrument and it gave no satisfaction (unsurprisingly). It DOES help reduce the charge loading on the grid, but at the expense of having carbon dust dispersed on the grid, which is not of the best effect.
Regards, Stephane
----- Original Message ---- X-from: "dagmorga-at-indiana.edu" {dagmorga-at-indiana.edu} To: nizets2-at-yahoo.com Sent: Thursday, December 18, 2008 12:06:36 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dagmorga-at-indiana.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dagmorga-at-indiana.edu Name: David Morgan
Organization: Indiana University
Title-Subject: [Filtered] carbon evaporator
Question: Hi,
Someone here is planning to purchase a Denton Desk IV carbon evaporator to use with a new SEM that is being installed in a few days. They are looking a the version of the system that does NOT have a turbo molecular pump and that is marketed as producing a larger grain size than the turbo pumped instrument. Does anyone have experience with using the Desk IV to make carbon foils for EM grids? I suspect that it doesn't work (or doesn't work well) for this purpose, but I'd like to hear from anyone with practical expeience.
I think the success of the manipulation depends on the surface you have to detach/separate. Some embed the coverslip upside-down on a resin-filled beem capsule or an eppi with the end cut off and they report a good success with liquid nitrogen. (Shamefully) I must say I never tried this way. I leave the coverslip in a 6W plate for example (or a small petri dish), then I glue an eppendorf with the end cut-off on the coverslip with a thin layer of resin (1 day curing), then I fill the eppendorf with resin. After polymerisation, I just pull the eppendorf off the resin with forceps and the eppendorf separates from the coverslip with a clean flat surface. Sometimes the surface is broken but anyway you have to reduce the surface by trimming to be able to ultracut so it doesn't really matter. Please be aware that you need a very hard resin to do that, otherwise it may not break at the interface with the coverslip but somewhere else. With this technique you can make up to 4 blocks out of 1 coverslip (18x18).
Now I must say something: recently I tried to flat embed directly at the bottom of a petri dish, without coverslip. The plastic of the petridish separates in no time from the resin in liquid nitrogen. The surface of the resin in NOT flat, because the surface of the petri dish is not flat either. So when you start to cut the surface on the ultramicrotome, you will have "dust" coming from the impect surface first. However, it seems that eucarytic cells do not grow in the grooves, so in the end you miss nothing because in this dust is only resin, no biological material. When your block becomes flat-and-shiny, there come the cells! So for me it works really well and it is much more straightforward than using coverslips. Please note that I use only Epon.
I hope my explanations where clear enough. I that can help you, I can better explain in french ;-)
regards,
Stephane
----- Original Message ---- X-from: "srijay-at-stanford.edu" {srijay-at-stanford.edu} To: nizets2-at-yahoo.com Sent: Thursday, December 18, 2008 12:06:36 AM
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Email: srijay-at-stanford.edu Name: Jay
Organization: Stanford University
Title-Subject: [Filtered] removal of glass coverslips
Question: Hi:
Can anybody tell me how to remove glass coverslips from the resin. I have tried plunging it in liquid nitrogen or putting it in boiling water and then immersing in liquid nitrogen and it does not work. I am not comfortable using hydroflouric acid - any other suggestions would be greatly appreciated.
Sorry to diverge from the original subject, but you said "While Protein A-gold is my, and many others', favorite - because it gives closer detection and only one gold (or less ;) ) will bind to each primary"
Please could you tell me why is it an advantage to have only 1 secondary bind a primary Ab? Multiple binding secondaries would increase the sensitivity no? If your fear is that secondaries would "displace" the localisation of the primary, meaning less precision in space, why would several secondaries be less precise than 1?
regards,
Stephane
----- Original Message ---- X-from: "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov} To: nizets2-at-yahoo.com Sent: Thursday, December 18, 2008 12:55:31 AM
Paula,
You really should know the source of your primary in order to decide which gold conjugate to use. While Protein A-gold is my, and many others', favorite - because it gives closer detection and only one gold (or less ;) ) will bind to each primary - Protein A only binds well to IgGs from rabbit, pig, guinea pig (as well as some from human). So no, it is not an all-around choice. You could, though, use a bridging antibody - e.g., a rabbit-anti-mouse between that mouse monoclonal and Protein A. In this case, PAG will be indeed your universal probe, but you'll need to buy other Abs to bridge.
Not meaning to hijack, but I would be really interested if somebody could comment here on which *primary* is be preferable in those cases when there is a choice? Or, should I say, *which polyclonal*, since we know polyclonal works better for immunoEM? Rabbit, guinea pig?.. Or, perhaps those IgYs from chicken - won't bind Protein A, but I remember hearing somewhere that chicken Abs tend to give "cleaner" labeling, while those from rabbit are among the "dirtiest"? Anybody?..
As for source, I don't think you can go wrong with any. I used conjugates bought from EMS/Aurion, as well as from Ted Pella/BBI, Jackson ImmunoResearch, and even some from Sigma - no problem ever with gold probe. Wanted to blame it on gold a few times, but always turned out to be my fault in the end :)
Vlad
________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and not his employer. These opinions and experiences do not represent a consensus of the NIH scientific community. On the good side, this message is not confidential and can be freely shared and reproduced.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Listers, } } I have a researcher who will be wanting immunoEM on cell pellets. } I'm going to embed them into LR White. } } What I want to know is what is the best type of gold to buy and from } who? The researcher isn't sure what the source of the primary will } be (rabbit, mouse, Sasquatch) so I was wondering if Protein A would } be the best all around choice. } } I haven't done immunoEM in a while so I was wondering what's the } latest and best in terms of optimizing the staining. Remember I } have to go with a room temp embedding media, I have no acccess to } low temp equipment. } } Thanks in advance. } } Does Rudolph's nose turn black when he has a cold? } } Paula :-) } } Paula Sicurello } VA Medical Center San Diego } Veterans Medical Research Foundation (VMRF) } Core Microscope Facility, room B141 } 3350 La Jolla Village Dr., MC151 } San Diego, CA 92161 } 858-552-8585 x2397 } } } } } } ==============================Original } Headers============================== } 11, 25 -- From vapatpxs-at-yahoo.com Wed Dec 17 12:05:19 2008 } 11, 25 -- Received: from n26.bullet.mail.mud.yahoo.com } (n26.bullet.mail.mud.yahoo.com [68.142.206.221]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
Hi Stephane and Jay- Don't use PO or acetonitrile .....I also embed a LOT of cell monolayers in multi-well plates, using pretty much the system you've described with coverslips. I go from the last 100% ethanol directly into a thin layer of the Epon-analog resin, let that stand in the hood for a couple of hours then insert tubes made by cutting the pyramidal ends off of BEEM capsules. I put them in the oven to polymerize overnight and in the morning fill up the tubes with more resin (inserting labels) and fully polymerize. Once hardened and cooled, I grasp the embedding tubes with a pair of needle-nosed pliers and snap them off. Sometimes a bit of the dish comes up, but there is always an expanse of very smooth block face. The one time that I got the rough surface you described was when I thought that I'd get better infiltration of the resin if I used a transition from EtOH via acetonitrile. I'd tested the acetonitrile on an empty dish and it didn't eat the plastic the way PO does, but it must have etched it slightly. My separated blocks had ridged faces. Try it. Happy Holidays and a great New Year to everyone out there, Lee
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-- Lee Cohen-Gould Electron & Optical Microscopy Core Facilities Weill Cornell Medical College (212)746-6146 Rms A-105, LC-207
Thanks to all who replied me or posted their suggestions/comments on the list server in response to my inquiry (posted on 12/5/2008) as under:
“We are starting a new FIB Lab and having some acoustic issues. I would like to get recommendations on what kind of acoustic material/product is the best for a FIB or a TEM Lab? Would someone like to share personal experiences, good or bad?? And/or recommend particular product/s and vendors??”
I further need experienced advise on the subject so, let me re-phrase my inquiry in more specific terms. I in fact am more curious in getting some feedback from experienced users on issues like 'lint' or 'particles' and 'rotting' and 'cracking' of the acoustic damping product/s in the long run. Please share your experiences, good or bad and recommend (if possible) some good products. Thanks.
Zia ur Rahman FIB Laboratory Manager, NASA Johnson Space Center, Houston, TX Email: Zia.rahman-1-at-nasa.gov
==============================Original Headers============================== 5, 30 -- From Zia.Rahman-1-at-nasa.gov Thu Dec 18 10:06:27 2008 5, 30 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov [198.117.0.123]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBIG6QJ7003200 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:27 -0600 5, 30 -- Received: from ndjsppt02.ndc.nasa.gov (ndjsppt02.ndc.nasa.gov [198.117.1.101]) 5, 30 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id D957E2D826F 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:25 -0600 (CST) 5, 30 -- Received: from ndjsxgw04.ndc.nasa.gov (ndjsxgw04.ndc.nasa.gov [129.166.32.112]) 5, 30 -- by ndjsppt02.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id mBIG6SjZ019296 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:28 -0600 5, 30 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by ndjsxgw04.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 5, 30 -- Thu, 18 Dec 2008 10:06:25 -0600 5, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 30 -- Content-class: urn:content-classes:message 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; 5, 30 -- charset="iso-8859-1" 5, 30 -- Subject: FIB/SEM/TEM Acoustic Damping in Labs. 5, 30 -- Date: Thu, 18 Dec 2008 10:06:24 -0600 5, 30 -- Message-ID: {B385F8289FD0B144A75B3A8C3E434E2F299572-at-NDJSEVS25A.ndc.nasa.gov} 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- Thread-Topic: FIB/SEM/TEM Acoustic Damping in Labs. 5, 30 -- Thread-Index: Aclg4WtwtNtJQvpKQgG6CfXamqIsMQAACKwZ 5, 30 -- References: {200812180722.mBI7Mg6G003862-at-ns.microscopy.com} 5, 30 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} 5, 30 -- To: {Microscopy-at-microscopy.com} 5, 30 -- X-OriginalArrivalTime: 18 Dec 2008 16:06:25.0486 (UTC) FILETIME=[93E9BEE0:01C9612A] 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBIG6QJ7003200 ==============================End of - Headers==============================
Protein A is the smaller molecule compared to a complete antibody molecule in a secondary antibody gold conjugate. It also binds in a designated area on the primary antibody. This, at least in theory, is advantageous for the resolution of the labeling...but I would like to add that in experiments done over 25 years ago, using a three step labeling with protein A/gold as the final step there was little doubt as to whether the epitope was on the outside or the inside of bacterial membranes, even though the detecting complex was all in all quite substantial and 'flexible'. Using a secondary antibody conjugate or antibody fragment (Fab or Fab2) might result in more gold particles per primary bound. But is this an increase in sensitivity? It certainly results in an increase in detectability, but if both protein A and a secondary antibody would recognize the same number of primary antibodies one still observes the same number of antigens.
To get a more complete image it is also necessary to take the binding forces into account which is likely more favourable with secondary conjugates because of avidity rather than affinity binding. This helps keeping the label in place during washing steps for instance. All this is also related to the size of the gold particles. But that's a different story all together.
Kind regards and a nice holiday season to all of you.
Jan Leunissen Aurion - http://www.aurion.nl
On 19/12/2008, at 3:45 AM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi! } } Sorry to diverge from the original subject, but you said "While } Protein A-gold is my, and many } others', favorite - because it gives closer detection and only one } gold (or less ;) ) will bind to each primary" } } Please could you tell me why is it an advantage to have only 1 } secondary bind a primary Ab? } Multiple binding secondaries would increase the sensitivity no? } If your fear is that secondaries would "displace" the localisation } of the primary, meaning less precision in space, why would several } secondaries be less precise than 1? } } regards, } } Stephane }
Regarding your comment: "Multiple binding secondaries would increase the sensitivity no?". This is not exactly the case.
By increasing the number of secondary antibodies binding to the initial bound antibody you only amplify the signal that is already there. To increase sensitivity, you have to bind more of the first antibody to the antigen. There are specific methods that aim to do this (e.g. antigen retrieval) but which occur before the antibodies (or affinity probes) are applied.
I agree with Vlad that protein A-gold is probably the best way of visualizing bound antibodies at the moment. Not only does PAG bind 1:1 with rabbit polyclonal antibodies, but it can be used with unconjugated secondary antibodies (which are less expensive and easier to store than conjugated antibodies) as a bridge for when non-protein A -binding primary antibodies are used.
Using one reagent for all visualization experiments needs makes it easier to monitor the reactivity of the reagent so that if one user finds the probe to be "not working", then the problem can be easily diagnosed, especially if the probe still works for all the other users (!!!).
Using protein A gold also means that fewer probes are needed. I have only PAG 5nm and PAG 10 nm in storage which I used for all immunocytochemical needs. Our primaries are made in rabbit, mouse, goat, chicken and donkey. If I used secondary antibodies I would need a very large collection of antibodies conjugated to gold, which do not store very well over long periods (5nm and 10 nm gold coupled to anti-goat, mouse, rabbit, etc).
Single gold particles (one gold per antigen), although thought to be insensitive when only a few are detected, can say much about the abundance of the antigen (fewer gold equals less antigen) and the location of the antigen. The small particles offer relatively high-resolution localization of antigens, which is a good thing.
Regards,
Paul.
Paul Webster, Ph.D House Ear Institute 2100 West Third Street Los Angeles, CA 90057 (213) 273 8026 pwebster-at-hei.org
} From: {nizets2-at-yahoo.com} } Reply-To: {nizets2-at-yahoo.com} } Date: Thu, 18 Dec 2008 08:47:40 -0600 } To: Paul Webster {PWebster-at-hei.org} } Subject: [Microscopy] Re: Fwd: LR White and gold } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi! } } Sorry to diverge from the original subject, but you said "While Protein A-gold } is my, and many } others', favorite - because it gives closer detection and only one gold (or } less ;) ) will bind to each primary" } } Please could you tell me why is it an advantage to have only 1 secondary bind } a primary Ab? } Multiple binding secondaries would increase the sensitivity no? } If your fear is that secondaries would "displace" the localisation of the } primary, meaning less precision in space, why would several secondaries be } less precise than 1? } } regards, } } Stephane } } } } ----- Original Message ---- } X-from: "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov} } To: nizets2-at-yahoo.com } Sent: Thursday, December 18, 2008 12:55:31 AM } Subject: [Microscopy] Fwd: LR White and gold } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Paula, } } You really should know the source of your primary in order to decide } which gold conjugate to use. While Protein A-gold is my, and many } others', favorite - because it gives closer detection and only one } gold (or less ;) ) will bind to each primary - Protein A only binds } well to IgGs from rabbit, pig, guinea pig (as well as some from } human). So no, it is not an all-around choice. You could, though, use } a bridging antibody - e.g., a rabbit-anti-mouse between that mouse } monoclonal and Protein A. In this case, PAG will be indeed your } universal probe, but you'll need to buy other Abs to bridge. } } Not meaning to hijack, but I would be really interested if somebody } could comment here on which *primary* is be preferable in those cases } when there is a choice? Or, should I say, *which polyclonal*, since we } know polyclonal works better for immunoEM? Rabbit, guinea pig?.. Or, } perhaps those IgYs from chicken - won't bind Protein A, but I remember } hearing somewhere that chicken Abs tend to give "cleaner" labeling, } while those from rabbit are among the "dirtiest"? } Anybody?.. } } As for source, I don't think you can go wrong with any. I used } conjugates bought from EMS/Aurion, as well as from Ted Pella/BBI, } Jackson ImmunoResearch, and even some from Sigma - no problem ever } with gold probe. Wanted to blame it on gold a few times, but always } turned out to be my fault in the end :) } } Vlad } } ________________________________________________ } Vlad Speransky, Staff Scientist } Supramolecular Structure and Function Resource } National Institute of Biomedical Imaging and Bioengineering, NIH } 13 South Dr, Rm. 3N17 MSC 5766 } Bethesda, MD 20892 } 301 496-3989 } vladislav_speransky-at-nih.gov } } Opinions and experiences related are those of Vlad Speransky and not } his employer. These opinions and experiences do not represent a } consensus of the NIH scientific community. } On the good side, this message is not confidential and can be freely } shared and reproduced. } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hello Listers, } } } } I have a researcher who will be wanting immunoEM on cell pellets. } } I'm going to embed them into LR White. } } } } What I want to know is what is the best type of gold to buy and from } } who? The researcher isn't sure what the source of the primary will } } be (rabbit, mouse, Sasquatch) so I was wondering if Protein A would } } be the best all around choice. } } } } I haven't done immunoEM in a while so I was wondering what's the } } latest and best in terms of optimizing the staining. Remember I } } have to go with a room temp embedding media, I have no acccess to } } low temp equipment. } } } } Thanks in advance. } } } } Does Rudolph's nose turn black when he has a cold? } } } } Paula :-) } } } } Paula Sicurello } } VA Medical Center San Diego } } Veterans Medical Research Foundation (VMRF) } } Core Microscope Facility, room B141 } } 3350 La Jolla Village Dr., MC151 } } San Diego, CA 92161 } } 858-552-8585 x2397 } } } } } } } } } } } } ==============================Original } } Headers============================== } } 11, 25 -- From vapatpxs-at-yahoo.com Wed Dec 17 12:05:19 2008 } } 11, 25 -- Received: from n26.bullet.mail.mud.yahoo.com } } (n26.bullet.mail.mud.yahoo.com [68.142.206.221]) } } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
We put Sonex Super Wedges in our lab, and they were just incredible at killing sound in the room. http://www.illbruck-sonex.com/products_SonexSuperWedges.php
However the stuff is not real durable. The 'peaks' keep breaking off little pieces if you bump the wall and it becomes rather unsightly. I know NCEM used a cloth covered material that is MUCH more durable, probably something like 'fabritec'
but I cannot vouch for the precise identity of their material.
John Mardinly, Numonyx
-----Original Message----- X-from: Zia.Rahman-1-at-nasa.gov [mailto:Zia.Rahman-1-at-nasa.gov] Sent: Thursday, December 18, 2008 8:13 AM To: MARDINLY, A
Thanks to all who replied me or posted their suggestions/comments on the list server in response to my inquiry (posted on 12/5/2008) as under:
"We are starting a new FIB Lab and having some acoustic issues. I would like to get recommendations on what kind of acoustic material/product is the best for a FIB or a TEM Lab? Would someone like to share personal experiences, good or bad?? And/or recommend particular product/s and vendors??"
I further need experienced advise on the subject so, let me re-phrase my inquiry in more specific terms. I in fact am more curious in getting some feedback from experienced users on issues like 'lint' or 'particles' and 'rotting' and 'cracking' of the acoustic damping product/s in the long run. Please share your experiences, good or bad and recommend (if possible) some good products. Thanks.
Zia ur Rahman FIB Laboratory Manager, NASA Johnson Space Center, Houston, TX Email: Zia.rahman-1-at-nasa.gov
==============================Original Headers============================== 5, 30 -- From Zia.Rahman-1-at-nasa.gov Thu Dec 18 10:06:27 2008 5, 30 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov [198.117.0.123]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBIG6QJ7003200 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:27 -0600 5, 30 -- Received: from ndjsppt02.ndc.nasa.gov (ndjsppt02.ndc.nasa.gov [198.117.1.101]) 5, 30 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id D957E2D826F 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:25 -0600 (CST) 5, 30 -- Received: from ndjsxgw04.ndc.nasa.gov (ndjsxgw04.ndc.nasa.gov [129.166.32.112]) 5, 30 -- by ndjsppt02.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id mBIG6SjZ019296 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:28 -0600 5, 30 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by ndjsxgw04.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 5, 30 -- Thu, 18 Dec 2008 10:06:25 -0600 5, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 30 -- Content-class: urn:content-classes:message 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; 5, 30 -- charset="iso-8859-1" 5, 30 -- Subject: FIB/SEM/TEM Acoustic Damping in Labs. 5, 30 -- Date: Thu, 18 Dec 2008 10:06:24 -0600 5, 30 -- Message-ID: {B385F8289FD0B144A75B3A8C3E434E2F299572-at-NDJSEVS25A.ndc.nasa.gov} 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- Thread-Topic: FIB/SEM/TEM Acoustic Damping in Labs. 5, 30 -- Thread-Index: Aclg4WtwtNtJQvpKQgG6CfXamqIsMQAACKwZ 5, 30 -- References: {200812180722.mBI7Mg6G003862-at-ns.microscopy.com} 5, 30 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} 5, 30 -- To: {Microscopy-at-microscopy.com} 5, 30 -- X-OriginalArrivalTime: 18 Dec 2008 16:06:25.0486 (UTC) FILETIME=[93E9BEE0:01C9612A] 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBIG6QJ7003200 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 29 -- From A.MARDINLY-at-numonyx.com Thu Dec 18 16:41:10 2008 17, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBIMfAbs024169 17, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 18 Dec 2008 16:41:10 -0600 17, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 17, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 70CFB1E8295; 17, 29 -- Thu, 18 Dec 2008 16:00:10 -0500 (EST) 17, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 17, 29 -- Thu, 18 Dec 2008 17:41:09 -0500 17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 29 -- Content-class: urn:content-classes:message 17, 29 -- MIME-Version: 1.0 17, 29 -- Content-Type: text/plain; 17, 29 -- charset="us-ascii" 17, 29 -- Subject: RE: [Microscopy] FIB/SEM/TEM Acoustic Damping in Labs. 17, 29 -- Date: Thu, 18 Dec 2008 17:41:07 -0500 17, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9C390DB-at-EXDRESBENMX012.numonyx.local} 17, 29 -- In-Reply-To: {200812181612.mBIGCfXN014940-at-ns.microscopy.com} 17, 29 -- X-MS-Has-Attach: 17, 29 -- X-MS-TNEF-Correlator: 17, 29 -- Thread-Topic: [Microscopy] FIB/SEM/TEM Acoustic Damping in Labs. 17, 29 -- Thread-Index: AclhK4i+SDfwrBvmSsCOrBckAcu7IQANSmcQ 17, 29 -- References: {200812181612.mBIGCfXN014940-at-ns.microscopy.com} 17, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 17, 29 -- To: {Zia.Rahman-1-at-nasa.gov} 17, 29 -- Cc: {Microscopy-at-Microscopy.com} 17, 29 -- X-OriginalArrivalTime: 18 Dec 2008 22:41:09.0485 (UTC) FILETIME=[B8AD31D0:01C96161] 17, 29 -- Content-Transfer-Encoding: 8bit 17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBIMfAbs024169 ==============================End of - Headers==============================
All materials marketed for acoustic noise dampening that I've seen in the past were not cleanroom-compatible and would either accumulate or generate particles and/or fibers. Of cause there is a possibility that something new came out in last couple of years...
Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
-----Original Message----- X-from: A.MARDINLY-at-numonyx.com [mailto:A.MARDINLY-at-numonyx.com] Sent: Thursday, December 18, 2008 5:42 PM To: vray-at-partbeamsystech.com
We put Sonex Super Wedges in our lab, and they were just incredible at killing sound in the room. http://www.illbruck-sonex.com/products_SonexSuperWedges.php
However the stuff is not real durable. The 'peaks' keep breaking off little pieces if you bump the wall and it becomes rather unsightly. I know NCEM used a cloth covered material that is MUCH more durable, probably something like 'fabritec'
but I cannot vouch for the precise identity of their material.
John Mardinly, Numonyx
-----Original Message----- X-from: Zia.Rahman-1-at-nasa.gov [mailto:Zia.Rahman-1-at-nasa.gov] Sent: Thursday, December 18, 2008 8:13 AM To: MARDINLY, A
Thanks to all who replied me or posted their suggestions/comments on the list server in response to my inquiry (posted on 12/5/2008) as under:
"We are starting a new FIB Lab and having some acoustic issues. I would like to get recommendations on what kind of acoustic material/product is the best for a FIB or a TEM Lab? Would someone like to share personal experiences, good or bad?? And/or recommend particular product/s and vendors??"
I further need experienced advise on the subject so, let me re-phrase my inquiry in more specific terms. I in fact am more curious in getting some feedback from experienced users on issues like 'lint' or 'particles' and 'rotting' and 'cracking' of the acoustic damping product/s in the long run. Please share your experiences, good or bad and recommend (if possible) some good products. Thanks.
Zia ur Rahman FIB Laboratory Manager, NASA Johnson Space Center, Houston, TX Email: Zia.rahman-1-at-nasa.gov
==============================Original Headers============================== 5, 30 -- From Zia.Rahman-1-at-nasa.gov Thu Dec 18 10:06:27 2008 5, 30 -- Received: from ndmsnpf03.ndc.nasa.gov (ndmsnpf03.ndc.nasa.gov [198.117.0.123]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBIG6QJ7003200 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:27 -0600 5, 30 -- Received: from ndjsppt02.ndc.nasa.gov (ndjsppt02.ndc.nasa.gov [198.117.1.101]) 5, 30 -- by ndmsnpf03.ndc.nasa.gov (Postfix) with ESMTP id D957E2D826F 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:25 -0600 (CST) 5, 30 -- Received: from ndjsxgw04.ndc.nasa.gov (ndjsxgw04.ndc.nasa.gov [129.166.32.112]) 5, 30 -- by ndjsppt02.ndc.nasa.gov (8.14.1/8.14.1) with ESMTP id mBIG6SjZ019296 5, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 10:06:28 -0600 5, 30 -- Received: from NDJSEVS25A.ndc.nasa.gov ([129.166.32.124]) by ndjsxgw04.ndc.nasa.gov with Microsoft SMTPSVC(6.0.3790.3959); 5, 30 -- Thu, 18 Dec 2008 10:06:25 -0600 5, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 30 -- Content-class: urn:content-classes:message 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; 5, 30 -- charset="iso-8859-1" 5, 30 -- Subject: FIB/SEM/TEM Acoustic Damping in Labs. 5, 30 -- Date: Thu, 18 Dec 2008 10:06:24 -0600 5, 30 -- Message-ID: {B385F8289FD0B144A75B3A8C3E434E2F299572-at-NDJSEVS25A.ndc.nasa.gov} 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- Thread-Topic: FIB/SEM/TEM Acoustic Damping in Labs. 5, 30 -- Thread-Index: Aclg4WtwtNtJQvpKQgG6CfXamqIsMQAACKwZ 5, 30 -- References: {200812180722.mBI7Mg6G003862-at-ns.microscopy.com} 5, 30 -- From: "Rahman, Zia (JSC-KR)[ESCG]" {Zia.Rahman-1-at-nasa.gov} 5, 30 -- To: {Microscopy-at-microscopy.com} 5, 30 -- X-OriginalArrivalTime: 18 Dec 2008 16:06:25.0486 (UTC) FILETIME=[93E9BEE0:01C9612A] 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBIG6QJ7003200 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 29 -- From A.MARDINLY-at-numonyx.com Thu Dec 18 16:41:10 2008 17, 29 -- Received: from smtp1.whdoakpoyel001.gmessaging.net (mail1.numonyx.com [57.77.12.37]) 17, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBIMfAbs024169 17, 29 -- for {Microscopy-at-Microscopy.com} ; Thu, 18 Dec 2008 16:41:10 -0600 17, 29 -- Received: from exdresfenmx03.numonyx.local (unknown [10.96.252.24]) 17, 29 -- by smtp1.whdoakpoyel001.gmessaging.net (Postfix) with ESMTP id 70CFB1E8295; 17, 29 -- Thu, 18 Dec 2008 16:00:10 -0500 (EST) 17, 29 -- Received: from EXDRESBENMX012.numonyx.local ([10.96.252.38]) by exdresfenmx03.numonyx.local with Microsoft SMTPSVC(6.0.3790.3959); 17, 29 -- Thu, 18 Dec 2008 17:41:09 -0500 17, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 29 -- Content-class: urn:content-classes:message 17, 29 -- MIME-Version: 1.0 17, 29 -- Content-Type: text/plain; 17, 29 -- charset="us-ascii" 17, 29 -- Subject: RE: [Microscopy] FIB/SEM/TEM Acoustic Damping in Labs. 17, 29 -- Date: Thu, 18 Dec 2008 17:41:07 -0500 17, 29 -- Message-ID: {21B544109D3D3E4380B776AC7CEA8CF9C390DB-at-EXDRESBENMX012.numonyx.local} 17, 29 -- In-Reply-To: {200812181612.mBIGCfXN014940-at-ns.microscopy.com} 17, 29 -- X-MS-Has-Attach: 17, 29 -- X-MS-TNEF-Correlator: 17, 29 -- Thread-Topic: [Microscopy] FIB/SEM/TEM Acoustic Damping in Labs. 17, 29 -- Thread-Index: AclhK4i+SDfwrBvmSsCOrBckAcu7IQANSmcQ 17, 29 -- References: {200812181612.mBIGCfXN014940-at-ns.microscopy.com} 17, 29 -- From: "MARDINLY, A" {A.MARDINLY-at-numonyx.com} 17, 29 -- To: {Zia.Rahman-1-at-nasa.gov} 17, 29 -- Cc: {Microscopy-at-Microscopy.com} 17, 29 -- X-OriginalArrivalTime: 18 Dec 2008 22:41:09.0485 (UTC) FILETIME=[B8AD31D0:01C96161] 17, 29 -- Content-Transfer-Encoding: 8bit 17, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBIMfAbs024169 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 25 -- From vray-at-partbeamsystech.com Thu Dec 18 18:03:07 2008 27, 25 -- Received: from smtp109.biz.mail.re2.yahoo.com (smtp109.biz.mail.re2.yahoo.com [206.190.53.8]) 27, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBJ036eF006835 27, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Dec 2008 18:03:06 -0600 27, 25 -- Message-Id: {200812190003.mBJ036eF006835-at-ns.microscopy.com} 27, 25 -- Received: (qmail 39399 invoked from network); 19 Dec 2008 00:03:06 -0000 27, 25 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) 27, 25 -- by smtp109.biz.mail.re2.yahoo.com with SMTP; 19 Dec 2008 00:03:05 -0000 27, 25 -- X-YMail-OSG: 8rYKmhwVM1l0lS8kgdDyWkRrR86BjQEF.085Tv.wZPUHXyDQGeblA1zXrDzbzijw4IQ.vSnW9jJzrpbA.MHllEqk4U40mnm03MGOcerRuii60R7fBjzgVlRt271rEoCM_RaaitFHvDczQys3mcNHDOUTWJ.2fQ016MwGoq3Ognm1v12Dtf1V4A4HrO5FktCY5W.eW5BEYS40Ql2W8S3JA8UoY6vgiD2X 27, 25 -- X-Yahoo-Newman-Property: ymail-3 27, 25 -- Reply-To: {vray-at-partbeamsystech.com} 27, 25 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 27, 25 -- To: {A.MARDINLY-at-numonyx.com} 27, 25 -- Cc: {microscopy-at-microscopy.com} 27, 25 -- Subject: RE: [Microscopy] RE: FIB/SEM/TEM Acoustic Damping in Labs. 27, 25 -- Date: Thu, 18 Dec 2008 19:05:49 -0500 27, 25 -- Organization: PBST / MEO Engineering 27, 25 -- MIME-Version: 1.0 27, 25 -- Content-Type: text/plain; 27, 25 -- charset="us-ascii" 27, 25 -- Content-Transfer-Encoding: 7bit 27, 25 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 27, 25 -- Thread-Index: AclhYd8h5TldW5nlQA2aqR04I4mpeQACdw0A 27, 25 -- In-Reply-To: {200812182242.mBIMgDTF025698-at-ns.microscopy.com} 27, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Regarding the sensitivity, I start with the hypothesis that the labeling will be used for quantification. It was my understanding (perhaps not right!?) that the amount of signal plays a role in the validation of quantification. Let's say that the amount of target protein is double in one cellular compartment in comparison with a second comparment.
Comp 1: 5 primaries Comp 2: 10 primaries
CASE 1 - If you have 1:1 secondary:primary this gives you:
Comp 1: 5 secondaries Comp 2: 10 secondaries
CASE 2 - If you have 10:1 secondaries:primary this gives you: Comp 1: 50 secondaries Comp 2: 100 secondaries
Given that all values are accompanied with standard deviations, chances are the SD in case 1 would make the difference not significant, while in case 2 the difference would be significant. Morevover, if you substract the background from case 1 it has a huge impact on the final amount, while in case 2 it has few impact.
I have not been trained for such things and I am the first to be sorry about it. My ideas come from a mixture of gut feeling and reasoning. I would be happy to be corrected if needed.
Regards,
Stephane
----- Original Message ---- X-from: "Webster, Paul" {PWebster-at-hei.org} To: nizets2-at-yahoo.com Cc: microscopy-at-microscopy.com Sent: Thursday, December 18, 2008 10:47:46 PM
Dear ListServers, following the thread by Giselle on straightening ultramicrotomed sections, I'd like to ask you what heat pen (manufacturer and model) you use effectively for this task. It's clear nobody's saying those ones are the best, just that they happened to be chosen among plenty of good heat pens that you can find around. Thanks to everybody
I am using a couple of mains powered heat pens which unfortunately are locked away in another building, at present. So I can't tell you their make/manufacturer.
I originally purchased them from a UK company called Agar Scientific, but when I check their web site they don't have this particular model any more, anyway. However they do something similar which has a variable heat control. I don't know the current price. but the item they now have is: B7962 HEAT PEN
you can see it on: http://www.agarscientific.com/catalogue then select "view on-line catalogue" then select "Specimen Preparation - Biological" then select "heat pen"
I'm not sure if Agar Scientific have a US branch or links with one of the US suppliers but obviously there would need to be some way of setting mains voltage from UK 230/240V to US standard.
The usual disclaimers apply - I have no connection with the company other than as a satisfied customer. Good luck and Merry Christmas.
Malcolm
Malcolm Haswell Electron Microscope Unit Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: dcristofori-at-unive.it
Dear All Has anybody used retipped filaments for a jeol 5600 with satisfactory results and can suggest to me the type of filaments used and company name(s)? Thanks Yorgos
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pgale-at-analytical services.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pgale-at-analytical services.com Name: Peter Gale
Title-Subject: [Filtered] KEVEX Delta Class video display
Question: I need to replace my dead KEVEX (1987) video display:
The KEVEX video display operates at 2 volts with the frequency of the horizontal at 17.153 Khz and the vertical at 57.9 hz. These numbers relate to the Sync Pulse Timing. The signal out is looking for a resistance of 75 ohms. The video signal from white level to black level is 0.7 volts.
The male RGBHV (5 plugs) comes out of the KEVEX box (PDP 11-23?) and plugs in to the back of the KEXEX video display (BNC female).
On Dec 19, 2008, at 1:02 AM, nizets2-at-yahoo.com wrote:
} Regarding the sensitivity, I start with the hypothesis that the } labeling will be used for quantification. } It was my understanding (perhaps not right!?) that the amount of } signal plays a role in the validation of quantification. } Let's say that the amount of target protein is double in one } cellular compartment in comparison with a second comparment. } } Comp 1: 5 primaries } Comp 2: 10 primaries } } CASE 1 - If you have 1:1 secondary:primary this gives you: } } Comp 1: 5 secondaries } Comp 2: 10 secondaries } } CASE 2 - If you have 10:1 secondaries:primary this gives you: } Comp 1: 50 secondaries } Comp 2: 100 secondaries } } Given that all values are accompanied with standard deviations, } chances are the SD in case 1 would make the difference not } significant, while in case 2 the difference would be significant. } Morevover, if you substract the background from case 1 it has a huge } impact on the final amount, while in case 2 it has few impact. } } I have not been trained for such things and I am the first to be } sorry about it. My ideas come from a mixture of gut feeling and } reasoning. } I would be happy to be corrected if needed.
Dear Stephane, Whether one gets an increase in signal/noise depends on a number of things. First, there may be a probability less than 1 for the primary to bind to the target even with a vast excess of primary in the reaction. This can be due to incomplete penetration into the region where the target is located, such as when the region is bounded with a membrane that is not too permeable to the primary. There may also be interactions of the target with other components of the region, such as formation of protein complexes, that compete with binding of the primary. Second, there may be similar issues with the secondary. Third, incomplete washout of either unbound primary or secondary will result in noise, so in either case 1 or 2 the noise could be large and variable. Suppose, for example, that either the unbound primary or secondary is washed out to 90%, but secondary bound to primary is only washed out to 50% (since it is a larger complex). One can imagine conditions where 10:1 secondary:primary will result in a large noise component that cannot be determined from using a non-binding control, e.g., preimmune serum, that does react with the secondary. In your example above with 5 and 10 targets in compartments 1 and 2 respectively, case 1 would give 5 x (probability of primary binding in Comp 1) primaries in Comp 1 and 10 x (probability of primary binding in Comp 2--not necessarily = that for Comp 1) primaries in Comp 2. Then using similar considerations for secondary binding and detection efficiencies, one would see signal(Comp 1) and signal(Comp 2) that are not necessarily 1:2 with either 1:1 or 10:1 secondaries:primary. In a somewhat related case--but not exactly the same--using a CCD to record TEM images one can have two phosphor screens one of which produces 5 photons per primary electron and the other of which produces 10 photons per primary electron. There is a SD for the number of photons per electron for each screen and an overall SD for photons in each pixel (which can be computed from the SDs for photons/ electron and for # of electrons). Although increasing photons/ electron will decrease the SD for that ratio, it will not decrease the SD for electrons, i.e. shot noise, so the increase in S/N will be limited by that shot noise. Increased photons/electron will make CCD readout noise negligible, so there is some gain to be had by increasing that ratio, but after a while that gain is not significant, which translates into the statement that the intensities for two compartments, as visualized by CCD signals, will not be distinguishable if the numbers of primary electrons are not distinguishable, regardless of the ratio of photons per primary electron. Yours, Bill Tivol, PhD EM Scientist Ultrafast EM Facility Noyes Laboratory, MC 127-72 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Fri Dec 19 15:40:02 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBJLe2qj031383 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 15:40:02 -0600 6, 22 -- Received: from earth-doxen.imss.caltech.edu (localhost [127.0.0.1]) 6, 22 -- by earth-doxen-postvirus (Postfix) with ESMTP id 2EB4066E0B2E 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 13:40:02 -0800 (PST) 6, 22 -- X-Spam-Scanned: at Caltech-IMSS on earth-doxen by amavisd-new 6, 22 -- Received: from DHCP-19-146.caltech.edu (DHCP-19-146.caltech.edu [131.215.19.146]) 6, 22 -- by earth-doxen-ssl (Postfix) with ESMTP id EA58466E0C18 6, 22 -- for {microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 13:40:00 -0800 (PST) 6, 22 -- Message-Id: {D8F0DE9D-A1B7-408A-BA72-838E25357512-at-caltech.edu} 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- In-Reply-To: {200812190902.mBJ92a25006650-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- Mime-Version: 1.0 (Apple Message framework v929.2) 6, 22 -- Subject: Sensitivity in antibody labeling 6, 22 -- Date: Fri, 19 Dec 2008 13:40:00 -0800 6, 22 -- References: {200812190902.mBJ92a25006650-at-ns.microscopy.com} 6, 22 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
We have a project that is to detect GFP and HA expression on Golgi apparatus of cultured cells that are transfected with GFP and HA tagged proteins. I have several related questions: 1, what kind of cell is easy to find nice Golgi apparatus? Cos cells and 293T cells are easy to be transfected, but we have hard time to find nice Golgi; we could easily find nice Golgi in MDCK cells, but the transfection efficiency is low in those cells. 2. Does anybody know a good source of anti-HA antibody that will work in preembedding or Tokoyasou method?
Thanks in advance,
Alice
-- Alice F. Liang, Ph.D. Image Core Facility New York University School of Medicine New York, NY 10016 http://saturn.med.nyu.edu/facilities/imagecore/
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==============================Original Headers============================== 9, 24 -- From Fengxia.Liang-at-med.nyu.edu Fri Dec 19 16:18:24 2008 9, 24 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBJMIOX3013258 9, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 16:18:24 -0600 9, 24 -- X-IronPort-AV: E=Sophos;i="4.36,250,1228107600"; 9, 24 -- d="scan'208";a="45217266" 9, 24 -- Received: from msgwsdcpbh61.nyumc.org ([10.147.36.107]) 9, 24 -- by mail4.nyumc.org with ESMTP; 19 Dec 2008 17:17:38 -0500 9, 24 -- Received: from MSGWSDCPMB02.nyumc.org ([10.147.37.225]) by msgwsdcpbh61.nyumc.org with Microsoft SMTPSVC(6.0.3790.3959); 9, 24 -- Fri, 19 Dec 2008 17:17:13 -0500 9, 24 -- Received: from 128.122.135.9 ([128.122.135.9]) by MSGWSDCPMB02.nyumc.org ([10.147.37.24]) via Exchange Front-End Server mail.nyumc.org ([10.134.254.78]) with Microsoft Exchange Server HTTP-DAV ; 9, 24 -- Fri, 19 Dec 2008 22:17:13 +0000 9, 24 -- User-Agent: Microsoft-Entourage/12.11.0.080522 9, 24 -- Date: Fri, 19 Dec 2008 17:17:10 -0500 9, 24 -- Subject: GFP and HA labeling on Golgi 9, 24 -- From: Alice Liang {Fengxia.Liang-at-med.nyu.edu} 9, 24 -- To: {Microscopy-at-microscopy.com} 9, 24 -- Message-ID: {C5718796.2F107%Fengxia.Liang-at-med.nyu.edu} 9, 24 -- Thread-Topic: GFP and HA labeling on Golgi 9, 24 -- Thread-Index: AcliJ4kWWO4x8+FYRF+wKZmfxBPdCw== 9, 24 -- Mime-version: 1.0 9, 24 -- X-OriginalArrivalTime: 19 Dec 2008 22:17:13.0340 (UTC) FILETIME=[8B149BC0:01C96227] 9, 24 -- Content-Transfer-Encoding: 7bit 9, 24 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Sorry I could not promptly respond to you question - and many thanks to Jan and Paul for stepping in!.. It is indeed a great point, the one about the advantage of having only one type of gold conjugate to keep track of.
I've just been meaning to point out what that "more than one gold per each primary" binding really looks like in the microscope. It looks like loose clusters, some kind of rosettes. If the antigen is in a bordered compartment - granules, Golgi cisterns - you'll see your gold "spill over". Not nice, if you are interested in higher resolution localization. For my first immunogold labeling, back in 2000, I used 6 nm GAR. When I saw the clusters, I first thought these were some storage-related aggregates of either primary or secondary Ab - until I tried using PAG instead.
Happy Holidays, peace and prosperity to everyone!
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Opinions and experiences related are those of Vlad Speransky and not his employer. These opinions and experiences do not represent a consensus of the NIH scientific community. On the good side, this message is not confidential and can be freely shared and reproduced.
Begin forwarded message:
} From: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } Date: December 19, 2008 4:03:43 AM EST } To: "Speransky, Vlad (NIH/NIBIB) [E]" {speransv-at-mail.nih.gov} } Subject: [Microscopy] Fwd: LR White and gold } Reply-To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Regarding the sensitivity, I start with the hypothesis that the } labeling will be used for quantification. } It was my understanding (perhaps not right!?) that the amount of } signal plays a role in the validation of quantification. } Let's say that the amount of target protein is double in one } cellular compartment in comparison with a second comparment. } } Comp 1: 5 primaries } Comp 2: 10 primaries } } CASE 1 - If you have 1:1 secondary:primary this gives you: } } Comp 1: 5 secondaries } Comp 2: 10 secondaries } } CASE 2 - If you have 10:1 secondaries:primary this gives you: } Comp 1: 50 secondaries } Comp 2: 100 secondaries } } Given that all values are accompanied with standard deviations, } chances are the SD in case 1 would make the difference not } significant, while in case 2 the difference would be significant. } Morevover, if you substract the background from case 1 it has a huge } impact on the final amount, while in case 2 it has few impact. } } I have not been trained for such things and I am the first to be } sorry about it. My ideas come from a mixture of gut feeling and } reasoning. } I would be happy to be corrected if needed. } } Regards, } } Stephane } } } } } ----- Original Message ---- } X-from: "Webster, Paul" {PWebster-at-hei.org} } To: nizets2-at-yahoo.com } Cc: microscopy-at-microscopy.com } Sent: Thursday, December 18, 2008 10:47:46 PM } Subject: Re: [Microscopy] Re: Fwd: LR White and gold } } Dear Stephane, } } Regarding your comment: "Multiple binding secondaries would increase } the } sensitivity no?". This is not exactly the case. } } By increasing the number of secondary antibodies binding to the } initial } bound antibody you only amplify the signal that is already there. To } increase sensitivity, you have to bind more of the first antibody to } the } antigen. There are specific methods that aim to do this (e.g. antigen } retrieval) but which occur before the antibodies (or affinity } probes) are } applied. } } I agree with Vlad that protein A-gold is probably the best way of } visualizing bound antibodies at the moment. Not only does PAG bind } 1:1 with } rabbit polyclonal antibodies, but it can be used with unconjugated } secondary } antibodies (which are less expensive and easier to store than } conjugated } antibodies) as a bridge for when non-protein A -binding primary } antibodies } are used. } } Using one reagent for all visualization experiments needs makes it } easier to } monitor the reactivity of the reagent so that if one user finds the } probe to } be "not working", then the problem can be easily diagnosed, } especially if } the probe still works for all the other users (!!!). } } Using protein A gold also means that fewer probes are needed. I have } only } PAG 5nm and PAG 10 nm in storage which I used for all } immunocytochemical } needs. Our primaries are made in rabbit, mouse, goat, chicken and } donkey. If } I used secondary antibodies I would need a very large collection of } antibodies conjugated to gold, which do not store very well over long } periods (5nm and 10 nm gold coupled to anti-goat, mouse, rabbit, etc). } } Single gold particles (one gold per antigen), although thought to be } insensitive when only a few are detected, can say much about the } abundance } of the antigen (fewer gold equals less antigen) and the location of } the } antigen. The small particles offer relatively high-resolution } localization } of antigens, which is a good thing. } } Regards, } } Paul. } } } } } Paul Webster, Ph.D } House Ear Institute } 2100 West Third Street } Los Angeles, CA 90057 } (213) 273 8026 } pwebster-at-hei.org } } } } From: {nizets2-at-yahoo.com} } } Reply-To: {nizets2-at-yahoo.com} } } Date: Thu, 18 Dec 2008 08:47:40 -0600 } } To: Paul Webster {PWebster-at-hei.org} } } Subject: [Microscopy] Re: Fwd: LR White and gold } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi! } } } } Sorry to diverge from the original subject, but you said "While } } Protein A-gold } } is my, and many } } others', favorite - because it gives closer detection and only one } } gold (or } } less ;) ) will bind to each primary" } } } } Please could you tell me why is it an advantage to have only 1 } } secondary bind } } a primary Ab? } } Multiple binding secondaries would increase the sensitivity no? } } If your fear is that secondaries would "displace" the localisation } } of the } } primary, meaning less precision in space, why would several } } secondaries be } } less precise than 1? } } } } regards, } } } } Stephane } } } } } } } } ----- Original Message ---- } } X-from: "vladislav_speransky-at-nih.gov" {vladislav_speransky-at-nih.gov} } } To: nizets2-at-yahoo.com } } Sent: Thursday, December 18, 2008 12:55:31 AM } } Subject: [Microscopy] Fwd: LR White and gold } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Paula, } } } } You really should know the source of your primary in order to decide } } which gold conjugate to use. While Protein A-gold is my, and many } } others', favorite - because it gives closer detection and only one } } gold (or less ;) ) will bind to each primary - Protein A only binds } } well to IgGs from rabbit, pig, guinea pig (as well as some from } } human). So no, it is not an all-around choice. You could, though, use } } a bridging antibody - e.g., a rabbit-anti-mouse between that mouse } } monoclonal and Protein A. In this case, PAG will be indeed your } } universal probe, but you'll need to buy other Abs to bridge. } } } } Not meaning to hijack, but I would be really interested if somebody } } could comment here on which *primary* is be preferable in those cases } } when there is a choice? Or, should I say, *which polyclonal*, since } } we } } know polyclonal works better for immunoEM? Rabbit, guinea pig?.. Or, } } perhaps those IgYs from chicken - won't bind Protein A, but I } } remember } } hearing somewhere that chicken Abs tend to give "cleaner" labeling, } } while those from rabbit are among the "dirtiest"? } } Anybody?.. } } } } As for source, I don't think you can go wrong with any. I used } } conjugates bought from EMS/Aurion, as well as from Ted Pella/BBI, } } Jackson ImmunoResearch, and even some from Sigma - no problem ever } } with gold probe. Wanted to blame it on gold a few times, but always } } turned out to be my fault in the end :) } } } } Vlad } } } } ________________________________________________ } } Vlad Speransky, Staff Scientist } } Supramolecular Structure and Function Resource } } National Institute of Biomedical Imaging and Bioengineering, NIH } } 13 South Dr, Rm. 3N17 MSC 5766 } } Bethesda, MD 20892 } } 301 496-3989 } } vladislav_speransky-at-nih.gov } } } } Opinions and experiences related are those of Vlad Speransky and not } } his employer. These opinions and experiences do not represent a } } consensus of the NIH scientific community. } } On the good side, this message is not confidential and can be freely } } shared and reproduced. } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hello Listers, } } } } } } I have a researcher who will be wanting immunoEM on cell pellets. } } } I'm going to embed them into LR White. } } } } } } What I want to know is what is the best type of gold to buy and from } } } who? The researcher isn't sure what the source of the primary will } } } be (rabbit, mouse, Sasquatch) so I was wondering if Protein A would } } } be the best all around choice. } } } } } } I haven't done immunoEM in a while so I was wondering what's the } } } latest and best in terms of optimizing the staining. Remember I } } } have to go with a room temp embedding media, I have no acccess to } } } low temp equipment. } } } } } } Thanks in advance. } } } } } } Does Rudolph's nose turn black when he has a cold? } } } } } } Paula :-) } } } } } } Paula Sicurello } } } VA Medical Center San Diego } } } Veterans Medical Research Foundation (VMRF) } } } Core Microscope Facility, room B141 } } } 3350 La Jolla Village Dr., MC151 } } } San Diego, CA 92161 } } } 858-552-8585 x2397 } } } } } } } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 11, 25 -- From vapatpxs-at-yahoo.com Wed Dec 17 12:05:19 2008 } } } 11, 25 -- Received: from n26.bullet.mail.mud.yahoo.com } } } (n26.bullet.mail.mud.yahoo.com [68.142.206.221]) } } } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
I've cut some hard specimens over the years but never sand. We have a researcher who wishes to look at a section of a sand grain to study the distribution of nanotubes on the surface.
Any suggestions on sectioning a grain of sand? Here's what I am planning:
embedding in hard Spurr resin old, 50 degree diamond knife 2 mm/sec cutting speed
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Oh, yeah, good luck with that! OK, what kind of sand? I immediately think coralline sand, then I have to back up and remember you have different (Si) sand. When I have sectioned plant parts that have contained silica bodies (e.g., pineapple epidermal cells) (don't ever agree to do this!), the particles have torn out of the resin, BUT have often left behind some surface pieces and, importantly for you, anything sort of stuck to the surface of the particle. So I expect you will have holey resin sections floating on your water and a little pile of sand at the bottom of your boat, but you may have some nanotubes left in the resin. Pick up on coated grids. Cross fingers.
Resort to high-resolution SEM. Come here; we are getting a new Hitachi S-4800 in two weeks with EDS and STEM, and it's 80F and NOT snowing!
Aloha, Tina
} I've cut some hard specimens over the years but never sand. We have a } researcher who wishes to look at a section of a sand grain to study } the distribution of nanotubes on the surface. } } Any suggestions on sectioning a grain of sand? Here's what I am planning: } } embedding in hard Spurr resin } old, 50 degree diamond knife } 2 mm/sec cutting speed } } Thanks, } } JB } } -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } Integrated Microscopy & Graphics Expertise (IMAGE) } Southern Illinois University } 750 Communications Drive - MC 4402 } Carbondale, IL 62901 } Telephone: 618-453-3730 } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } ==============================Original Headers============================== } 8, 17 -- From bozzola-at-siu.edu Fri Dec 19 17:19:43 2008 } 8, 17 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3]) } 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBJNJhJX008553 } 8, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 17:19:43 -0600 } 8, 17 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136]) } 8, 17 -- by cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP id mBJNJe5s027174 } 8, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 17:19:42 -0600 (CST) } 8, 17 -- Mime-Version: 1.0 } 8, 17 -- Message-Id: {a06240812c571db4cd407-at-[131.230.177.136]} } 8, 17 -- Date: Fri, 19 Dec 2008 17:19:36 -0600 } 8, 17 -- To: Microscopy-at-microscopy.com } 8, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 8, 17 -- Subject: TEM: sectioning sand } 8, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } 8, 17 -- X-Spam-Score: 0.00% } 8, 17 -- X-MASF: 0.00% } 8, 17 -- X-Whitelist: 0.00% } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Sat Dec 20 11:58:32 2008 7, 21 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBKHwWGj015492 7, 21 -- for {Microscopy-at-microscopy.com} ; Sat, 20 Dec 2008 11:58:32 -0600 7, 21 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id mBKHwRsw013337 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 21 -- Sat, 20 Dec 2008 07:58:28 -1000 (HST) 7, 21 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id mBKHwQXA013334; 7, 21 -- Sat, 20 Dec 2008 07:58:27 -1000 (HST) 7, 21 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Sat, 20 Dec 2008 07:58:26 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-Sender: tina-at-halia 7, 21 -- To: bozzola-at-siu.edu 7, 21 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] TEM: sectioning sand 7, 21 -- In-Reply-To: {200812192320.mBJNKWhL010034-at-ns.microscopy.com} 7, 21 -- Message-ID: {Pine.GSO.4.21.0812200746440.13293-100000-at-halia} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
I am starting to look for a replacement of my (no longer gracefully aging) CM-12. I am doing mostly standard biological TEM work and do not need greater than 100KEV. I think my only requirement is a good digital camera. If people would be willing to share their recent experiences and research into the various options out there, I would be happy to summarize the results for the list.
Thank you for your help and knowledge David
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 8, 20 -- From Elliott-at-arizona.edu Sun Dec 21 15:12:54 2008 8, 20 -- Received: from smtpgate.email.arizona.edu (gandalf.email.arizona.edu [128.196.133.169]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBLLCsHo021204 8, 20 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 21 Dec 2008 15:12:54 -0600 8, 20 -- Received: from gandalfs_amavis (amavis8.email.arizona.edu [10.0.0.236]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 2248189E609 8, 20 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 21 Dec 2008 14:12:54 -0700 (MST) 8, 20 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 0BE789952B7 8, 20 -- for {MICROSCOPY-at-ns.microscopy.com} ; Sun, 21 Dec 2008 14:12:51 -0700 (MST) 8, 20 -- Message-Id: {65C0D984-B12B-496C-89FB-3F032516E243-at-arizona.edu} 8, 20 -- From: David Elliott {Elliott-at-arizona.edu} 8, 20 -- To: Microscopy ListServer {MICROSCOPY-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- Mime-Version: 1.0 (Apple Message framework v929.2) 8, 20 -- Subject: biological TEM info request 8, 20 -- Date: Sun, 21 Dec 2008 14:12:49 -0700 8, 20 -- X-Mailer: Apple Mail (2.929.2) 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
I'm working on EBSD scan data collection and bumping against a road block about whether the data is statistically significant to be used for data analysis.
In my current situation, I am analyzing Al thin films for grains characteristics. The basic values of interest are total grains, edge grains (those not used in analysis) and average grain size. So, how would one converge on a reasonable range of total grains and edge grains given the largest grain size and a good confidence index?
As an example, I call your attention to a recent article in Microscopy Today. "Using EBSD to Map Domain Structures in Ferroelectrics," September 2008, pg 18-19.
This article has maps with huge data points and poorly defined edges. Is this due to poor data collection (scan summary data was not provided) or is it due to analysis in poor vacuum systems?
My work is all high vacuum so I see these reported results as failures if I did them. But is this typical of ESEM results? And regardless of the vacuum, what constitutes statistically significant data?
Any thoughts on this?
gary g.
==============================Original Headers============================== 9, 17 -- From gary-at-gaugler.com Sun Dec 21 16:33:22 2008 9, 17 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBLMXKA8003708 9, 17 -- for {microscopy-at-microscopy.com} ; Sun, 21 Dec 2008 16:33:21 -0600 9, 17 -- Message-Id: {200812212233.mBLMXKA8003708-at-ns.microscopy.com} 9, 17 -- Received: (qmail 17876 invoked from network); 21 Dec 2008 14:32:27 -0800 9, 17 -- Received: by simscan 1.1.0 ppid: 17873, pid: 17874, t: 0.1565s 9, 17 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 17 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 17 -- by smtp1 with SMTP; 21 Dec 2008 14:32:27 -0800 9, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 17 -- Date: Sun, 21 Dec 2008 14:33:07 -0800 9, 17 -- To: MSA listserver {microscopy-at-microscopy.com} 9, 17 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 17 -- Subject: EBSD data-statistically significant 9, 17 -- Mime-Version: 1.0 9, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I am about to bin the photographic unit that used to be fitted to my JSM 840A, I don't know the correct name of it but it's the substantial unit which hinges onto the control console, and has in it a smallish CRT tube and a Polaroid 545 'camera'. It hurts to put it into landfill.
I don't suppose anyone wants the entire unit (you pay shipping, from New Zealand), but I'm prepared to extract whatever part anyone might want, to reduce shipping costs.
all the best for the festive season and beyond
cheers
Ritchie Sims Auckland, NZ
==============================Original Headers============================== 6, 26 -- From r.sims-at-auckland.ac.nz Sun Dec 21 20:19:04 2008 6, 26 -- Received: from mailhost.auckland.ac.nz (larry.its.auckland.ac.nz [130.216.12.34]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBM2IxXd025651 6, 26 -- for {microscopy-at-Microscopy.Com} ; Sun, 21 Dec 2008 20:19:04 -0600 6, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 6, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 759FC192EB 6, 26 -- for {microscopy-at-Microscopy.Com} ; Mon, 22 Dec 2008 15:18:58 +1300 (NZDT) 6, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz 6, 26 -- Received: from mailhost.auckland.ac.nz ([127.0.0.1]) 6, 26 -- by localhost (larry.its.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id 5rnwNmX1SMt5 for {microscopy-at-Microscopy.Com} ; 6, 26 -- Mon, 22 Dec 2008 15:18:58 +1300 (NZDT) 6, 26 -- Received: from [130.216.59.18] (r.sims.glg.auckland.ac.nz [130.216.59.18]) 6, 26 -- by mailhost.auckland.ac.nz (Postfix) with ESMTP id 51D48192DB 6, 26 -- for {microscopy-at-Microscopy.Com} ; Mon, 22 Dec 2008 15:18:58 +1300 (NZDT) 6, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 6, 26 -- To: microscopy-at-Microscopy.Com 6, 26 -- Date: Mon, 22 Dec 2008 15:20:36 +1300 6, 26 -- MIME-Version: 1.0 6, 26 -- Subject: Free JEOL photographic unit 6, 26 -- Message-ID: {494FB044.3583.E91281-at-r.sims.auckland.ac.nz} 6, 26 -- Priority: normal 6, 26 -- X-mailer: Pegasus Mail for Windows (4.41) 6, 26 -- Content-type: text/plain; charset=US-ASCII 6, 26 -- Content-transfer-encoding: 7BIT 6, 26 -- Content-description: Mail message body ==============================End of - Headers==============================
Hi John, For sectioning sand make sure you borrow a diamond knife from the director;) Oops, you are the director! Never mind;) Seriously, ask the researcher to provide you with their diamond knife cause sand/silt is hell on a knife.
I've sectioned forams - they agglutinate sand/silt to form their shell (test). We embedded them with EMS' Araldite Embed 812. I can send you pdf's of the papers if you want to see micrographs. The 'sand' doesn't remain intact when it is sectioned - it fractures extensively.
Have you considered ion beam milling? Maybe that would be the way to go. I've never done that but perhaps others could comment on ion milling.
Happy Holidays! Beth
On Dec 19, 2008, at 6:20 PM, bozzola-at-siu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I've cut some hard specimens over the years but never sand. We have a } researcher who wishes to look at a section of a sand grain to study } the distribution of nanotubes on the surface. } } Any suggestions on sectioning a grain of sand? Here's what I am } planning: } } embedding in hard Spurr resin } old, 50 degree diamond knife } 2 mm/sec cutting speed } } Thanks, } } JB } } -- } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } John J. Bozzola, Ph.D., Director } Integrated Microscopy & Graphics Expertise (IMAGE) } Southern Illinois University } 750 Communications Drive - MC 4402 } Carbondale, IL 62901 } Telephone: 618-453-3730 } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } ==============================Original } Headers============================== } 8, 17 -- From bozzola-at-siu.edu Fri Dec 19 17:19:43 2008 } 8, 17 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu } [131.230.1.3]) } 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mBJNJhJX008553 } 8, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 17:19:43 } -0600 } 8, 17 -- Received: from [131.230.177.136] } (ws177136.microscope.siu.edu [131.230.177.136]) } 8, 17 -- by cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP } id mBJNJe5s027174 } 8, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 17:19:42 } -0600 (CST) } 8, 17 -- Mime-Version: 1.0 } 8, 17 -- Message-Id: {a06240812c571db4cd407-at-[131.230.177.136]} } 8, 17 -- Date: Fri, 19 Dec 2008 17:19:36 -0600 } 8, 17 -- To: Microscopy-at-microscopy.com } 8, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } 8, 17 -- Subject: TEM: sectioning sand } 8, 17 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } 8, 17 -- X-Spam-Score: 0.00% } 8, 17 -- X-MASF: 0.00% } 8, 17 -- X-Whitelist: 0.00% } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 21 -- From beth-at-plantbio.uga.edu Mon Dec 22 09:01:36 2008 8, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBMF1ap4001928 8, 21 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 09:01:36 -0600 8, 21 -- Received: from [128.192.26.46] ([128.192.26.46]) 8, 21 -- (authenticated user beth-at-plantbio.uga.edu) 8, 21 -- by dogwood.plantbio.uga.edu 8, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 8, 21 -- Mon, 22 Dec 2008 10:01:31 -0500 8, 21 -- Message-Id: {040551A0-D1E2-4C3B-BB37-2E92A0AE6DF0-at-plantbio.uga.edu} 8, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 8, 21 -- To: "Bozzola John. J." {bozzola-at-siu.edu} , 8, 21 -- microscopy microscopy {microscopy-at-microscopy.com} 8, 21 -- In-Reply-To: {200812192320.mBJNKG7k009477-at-ns.microscopy.com} 8, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- Mime-Version: 1.0 (Apple Message framework v929.2) 8, 21 -- Subject: Re: [Microscopy] TEM: sectioning sand 8, 21 -- Date: Mon, 22 Dec 2008 10:01:33 -0500 8, 21 -- References: {200812192320.mBJNKG7k009477-at-ns.microscopy.com} 8, 21 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
I'm not sure of the specific brand, but I use ones sold by Ted Pella and EMS. But, I also use surgical pens used to cauterize wounds and incisions. These work fine, and may be cheaper.
Phil
} Dear ListServers, } following the thread by Giselle on straightening ultramicrotomed } sections, I'd like to ask you what heat pen (manufacturer and model) you } use effectively for this task. } It's clear nobody's saying those ones are the best, just that they } happened to be chosen among plenty of good heat pens that you can find } around. } Thanks to everybody } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = +39.041.234.67.26 } e-mail = dcristofori[at]unive.it } } Universita' Ca' Foscari Venezia } Dipartimento di Chimica Fisica } Lab. di Scienza e Tecnologia dei Materiali } Via Torino, 155b } I-30172 Mestre (VE) } Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 27 -- From oshel1pe-at-cmich.edu Mon Dec 22 09:16:39 2008 3, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBMFGd8I015738 3, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 09:16:39 -0600 3, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 27 -- by ob4.cmich.edu (8.13.8/8.13.8/Debian-3) with ESMTP id mBMFGa4A003419; 3, 27 -- Mon, 22 Dec 2008 10:16:38 -0500 3, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.3959); 3, 27 -- Mon, 22 Dec 2008 10:15:52 -0500 3, 27 -- Mime-Version: 1.0 3, 27 -- Message-Id: {f06240806c5755f5c04e2-at-[141.209.160.249]} 3, 27 -- In-Reply-To: {200812191000.mBJA0X2O028794-at-ns.microscopy.com} 3, 27 -- References: {200812191000.mBJA0X2O028794-at-ns.microscopy.com} 3, 27 -- Date: Mon, 22 Dec 2008 10:15:48 -0500 3, 27 -- To: dcristofori-at-unive.it 3, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 27 -- Subject: Re: [Microscopy] TEM & Ultramicrotomy: what heat pen models do 3, 27 -- you use? 3, 27 -- Cc: Microscopy-at-microscopy.com 3, 27 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 27 -- X-OriginalArrivalTime: 22 Dec 2008 15:15:52.0433 (UTC) FILETIME=[2DB99610:01C96448] 3, 27 -- X-Canit-CHI2: 0.00 3, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 27 -- X-Spam-Score: -4.20 () [Hold at 5.00] L_EXCH_MF,L_USD,RDNS_NONE,Bayes(0.0001,-0.5) 3, 27 -- X-CanItPRO-Stream: default 3, 27 -- X-Canit-Stats-ID: 6855403 - b5a4fc76f672 3, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I suspect that microtoming sand (esp. quartz grains) would be as impossible as microtoming Si because of hardness and cleaving concerns.
Look through the ads in Microscopy Today for the vendors that sell instruments for ion prepping specimens. Any of them would be an excellent choice.
Making friends with someone who owns a FIB would be another good idea for either SEM or TEM specimen prep of sand grains.
Contact the instrument vendors and offer to co-author an article on electron microscopy of sand grains for Microscopy Today. I suspect it has never been done as their hasn't been any references to the literature so far. This might get you a foot in some manufacturer's app. lab.
Ron Anderson
beth-at-plantbio.uga.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John, } For sectioning sand make sure you borrow a diamond knife from the } director;) } Oops, you are the director! Never mind;) } Seriously, ask the researcher to provide you with their diamond knife } cause sand/silt is hell on a knife. } } I've sectioned forams - they agglutinate sand/silt to form their shell } (test). We embedded them with EMS' Araldite Embed 812. I can send you } pdf's of the papers if you want to see micrographs. The 'sand' doesn't } remain intact when it is sectioned - it fractures extensively. } } Have you considered ion beam milling? Maybe that would be the way to } go. I've never done that but perhaps others could comment on ion } milling. } } Happy Holidays! } Beth } } On Dec 19, 2008, at 6:20 PM, bozzola-at-siu.edu wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } I've cut some hard specimens over the years but never sand. We have a } } researcher who wishes to look at a section of a sand grain to study } } the distribution of nanotubes on the surface. } } } } Any suggestions on sectioning a grain of sand? Here's what I am } } planning: } } } } embedding in hard Spurr resin } } old, 50 degree diamond knife } } 2 mm/sec cutting speed } } } } Thanks, } } } } JB } } } } -- } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } } } John J. Bozzola, Ph.D., Director } } Integrated Microscopy & Graphics Expertise (IMAGE) } } Southern Illinois University } } 750 Communications Drive - MC 4402 } } Carbondale, IL 62901 } } Telephone: 618-453-3730 } } } } +++++++++++++++++++++++++++++++++++++++++++++++++++++++ } } } } ==============================Original } } Headers============================== } } 8, 17 -- From bozzola-at-siu.edu Fri Dec 19 17:19:43 2008 } } 8, 17 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu } } [131.230.1.3]) } } 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id mBJNJhJX008553 } } 8, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 17:19:43 } } -0600 } } 8, 17 -- Received: from [131.230.177.136] } } (ws177136.microscope.siu.edu [131.230.177.136]) } } 8, 17 -- by cstmta3.siu.edu (Switch-3.3.2/Switch-3.3.2) with ESMTP } } id mBJNJe5s027174 } } 8, 17 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 17:19:42 } } -0600 (CST) } } 8, 17 -- Mime-Version: 1.0 } } 8, 17 -- Message-Id: {a06240812c571db4cd407-at-[131.230.177.136]} } } 8, 17 -- Date: Fri, 19 Dec 2008 17:19:36 -0600 } } 8, 17 -- To: Microscopy-at-microscopy.com } } 8, 17 -- From: "John J. Bozzola" {bozzola-at-siu.edu} } } 8, 17 -- Subject: TEM: sectioning sand } } 8, 17 -- Content-Type: text/plain; charset="us-ascii" ; } } format="flowed" } } 8, 17 -- X-Spam-Score: 0.00% } } 8, 17 -- X-MASF: 0.00% } } 8, 17 -- X-Whitelist: 0.00% } } ==============================End of - } } Headers============================== } } } } } } ==============================Original Headers============================== } 8, 21 -- From beth-at-plantbio.uga.edu Mon Dec 22 09:01:36 2008 } 8, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBMF1ap4001928 } 8, 21 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 09:01:36 -0600 } 8, 21 -- Received: from [128.192.26.46] ([128.192.26.46]) } 8, 21 -- (authenticated user beth-at-plantbio.uga.edu) } 8, 21 -- by dogwood.plantbio.uga.edu } 8, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); } 8, 21 -- Mon, 22 Dec 2008 10:01:31 -0500 } 8, 21 -- Message-Id: {040551A0-D1E2-4C3B-BB37-2E92A0AE6DF0-at-plantbio.uga.edu} } 8, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 8, 21 -- To: "Bozzola John. J." {bozzola-at-siu.edu} , } 8, 21 -- microscopy microscopy {microscopy-at-microscopy.com} } 8, 21 -- In-Reply-To: {200812192320.mBJNKG7k009477-at-ns.microscopy.com} } 8, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes } 8, 21 -- Content-Transfer-Encoding: 7bit } 8, 21 -- Mime-Version: 1.0 (Apple Message framework v929.2) } 8, 21 -- Subject: Re: [Microscopy] TEM: sectioning sand } 8, 21 -- Date: Mon, 22 Dec 2008 10:01:33 -0500 } 8, 21 -- References: {200812192320.mBJNKG7k009477-at-ns.microscopy.com} } 8, 21 -- X-Mailer: Apple Mail (2.929.2) } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 8, 19 -- From randerson20-at-tampabay.rr.com Mon Dec 22 13:36:32 2008 8, 19 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBMJaVl0004737 8, 19 -- for {Microscopy-at-Microscopy.Com} ; Mon, 22 Dec 2008 13:36:32 -0600 8, 19 -- Received: from [127.0.0.1] (really [24.73.73.214]) 8, 19 -- by hrndva-omta01.mail.rr.com with ESMTP 8, 19 -- id {20081222193630.EGMU2065.hrndva-omta01.mail.rr.com-at-[127.0.0.1]} ; 8, 19 -- Mon, 22 Dec 2008 19:36:30 +0000 8, 19 -- Message-ID: {494FEC35.4050308-at-tampabay.rr.com} 8, 19 -- Date: Mon, 22 Dec 2008 14:36:21 -0500 8, 19 -- From: Ron Anderson {randerson20-at-tampabay.rr.com} 8, 19 -- User-Agent: Thunderbird 2.0.0.18 (Windows/20081105) 8, 19 -- MIME-Version: 1.0 8, 19 -- To: beth-at-plantbio.uga.edu, Listserver {Microscopy-at-Microscopy.Com} 8, 19 -- Subject: Re: [Microscopy] Re: TEM: sectioning sand 8, 19 -- References: {200812221501.mBMF1kQg002091-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200812221501.mBMF1kQg002091-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Dr Faerber Thank you very much for your useful comments. I´ll try Agar Scientific and see how they work in my scope. Best wishes for holidays yorgos
El 22/12/08 9:22, "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} escribió:
} I don't remember of a difference in the image quality, between the } retipped and the jeol. I must say that I have much forgotten about these } questions, as I work on FEG since 7 years now ! } The differences I notice were : } -the quality of the initial centering and it evolution during the } filament's life. We needed sometimes to recenter mechanically the } filament, in the middle of its life, being in the limit of the } electronical tilt and shift corrections. } - the difference in the lifetime. Some of then worked only 20 h, } other up to 200 h ! The jeol were always 35-60 h, never less. It was } really a batch questions. I remember from one batch that I sent back, as } the 2 or 3 first of the lot of 12 held only 20 h. But I had once a batch } too where they held 160-200 h ! } } Of coarse, a bad centering may have an incidence on the beam geometry, } wehnelt polarisation, position of the cross-over, etc., all that can } affect the image quality. As at low probe size one have a lower current } and so a bad S/N ratio, all the deffects of the beam will be better seen. } } Hope it helps } - } } J. Faerber } IPCMS-GSI } (Institut de Physique et Chimie des Matériaux de Strasbourg } Groupe Surface et Interfaces) } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } yorgos nikas a écrit : } } Dear Dr Faerber } } Merci a votre information. I have tried K type from energy beam sciences in } } the USA but they give a grainy image at low probe sizes, as compared to the } } Jeol ones. Have you noticed any such difference with the Agar ones? } } Kind regards } } Yorgos } } } } Dr Yorgos Nikas } } Athens Innovative Microscopy } } Skra 36, Voula 16673, Greece } } } } } } } } El 19/12/08 16:01, "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } } escribió: } } } } } } } If they are K-type filament, same type than in the JSM840, I sent them } } } to be retiped to Agar Scientific (in France it through the Oxford } } } Instrument representation). } } } Not always satisfied, somtimes they needed to be a bit recentered, what } } } was never the case with Jeol genuine parts, but much much less } } } expensive. Performences and life time are as good. } } } You schould find them in the Agar catalogue, under the "Jeol K-type" name. } } } } } } Hope it helps } } } } } } J. Faerber } } } IPCMS-GSI } } } (Institut de Physique et Chimie des Matériaux de Strasbourg } } } Groupe Surface et Interfaces) } } } 23, rue de Loess ; BP43 } } } 67034 Strasbourg CEDEX 2 } } } France } } } } } } Tel 00 33(0)3 88 10 71 01 } } } Fax 00 33(0)3 88 10 72 48 } } } E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr } } } } } } } } } } } } eikonika-at-otenet.gr a écrit : } } } } } } } ---------------------------------------------------------------------------} } } } - } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------} } } } - } } } } } } } } Dear All } } } } Has anybody used retipped filaments for a jeol 5600 with satisfactory } } } } results and can suggest to me the type of filaments used and company } } } } name(s)? } } } } Thanks } } } } Yorgos } } } } } } } } Dr Yorgos Nikas } } } } Athens Innovative Microscopy } } } } Skra 36, Voula 16673, Greece } } } } } } } } } } } } } } } } } } } } } } } } } } } } ==============================Original } } } } Headers============================== } } } } 7, 20 -- From eikonika-at-otenet.gr Fri Dec 19 07:19:52 2008 } } } } 7, 20 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr [83.235.67.30]) } } } } 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } } mBJDJoCO023358 } } } } 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 07:19:52 -0600 } } } } 7, 20 -- Received: from [192.168.1.36] } } } } (142.Red-88-10-186.dynamicIP.rima-tde.net [88.10.186.142]) } } } } 7, 20 -- (authenticated bits=0) } } } } 7, 20 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3) with ESMTP id } } } } mBJDJd1l025497 } } } } 7, 20 -- for {microscopy-at-microscopy.com} ; Fri, 19 Dec 2008 15:19:45 +0200 } } } } 7, 20 -- User-Agent: Microsoft-Entourage/12.10.0.080409 } } } } 7, 20 -- Date: Fri, 19 Dec 2008 14:19:37 +0100 } } } } 7, 20 -- Subject: Retipped filaments } } } } 7, 20 -- From: yorgos nikas {eikonika-at-otenet.gr} } } } } 7, 20 -- To: {microscopy-at-microscopy.com} } } } } 7, 20 -- Message-ID: {C5715DF9.2139%eikonika-at-otenet.gr} } } } } 7, 20 -- Thread-Topic: Retipped filaments } } } } 7, 20 -- Thread-Index: Aclh3HDNnNbfZP9VaU2edrSzsF8Ywg== } } } } 7, 20 -- Mime-version: 1.0 } } } } 7, 20 -- Content-type: text/plain; } } } } 7, 20 -- charset="US-ASCII" } } } } 7, 20 -- Content-transfer-encoding: 7bit } } } } ==============================End of - } } } } Headers============================== } } } } } } } } } } } } } } }
==============================Original Headers============================== 7, 23 -- From eikonika-at-otenet.gr Mon Dec 22 13:54:33 2008 7, 23 -- Received: from rosebud.otenet.gr (rosebud.otenet.gr [83.235.67.32]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBMJsV9h018568 7, 23 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 13:54:32 -0600 7, 23 -- Received: from [192.168.1.33] (191.Red-88-4-240.staticIP.rima-tde.net [88.4.240.191]) 7, 23 -- (authenticated bits=0) 7, 23 -- by rosebud.otenet.gr (8.13.8/8.13.8/Debian-3) with ESMTP id mBMJsN9Y019250; 7, 23 -- Mon, 22 Dec 2008 21:54:24 +0200 7, 23 -- User-Agent: Microsoft-Entourage/12.10.0.080409 7, 23 -- Date: Mon, 22 Dec 2008 20:54:22 +0100 7, 23 -- Subject: Re: [Microscopy] Retipped filaments 7, 23 -- From: yorgos nikas {eikonika-at-otenet.gr} 7, 23 -- To: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 7, 23 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 23 -- Message-ID: {C575AEFE.21AD%eikonika-at-otenet.gr} 7, 23 -- Thread-Topic: [Microscopy] Retipped filaments 7, 23 -- Thread-Index: AclkbxVntIjZCNstrku7bVpZD+o0vw== 7, 23 -- In-Reply-To: {494F4E35.8000101-at-ipcms.u-strasbg.fr} 7, 23 -- Mime-version: 1.0 7, 23 -- Content-type: text/plain; 7, 23 -- charset="ISO-8859-1" 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBMJsV9h018568 ==============================End of - Headers==============================
We have an Olympus-SIS CCD camera and their iTEM software on a Philips CM200 TEM and have found that the "on-line" indexing of diffraction patterns option leaves a lot to be desired in terms of consistency and accuracy. Thus, we are curious as to whether anyone in the EM community uses this software successfully for this purpose and, if so, if there are some tricks that we may have not yet discovered.
Thanks,
--John
John Chandler Colorado School of Mines jpchandl-at-mines.edu 303.384.2203 (office)
==============================Original Headers============================== 9, 19 -- From jpchandl-at-mines.edu Mon Dec 22 15:55:55 2008 9, 19 -- Received: from inception.Mines.EDU (inception.Mines.EDU [138.67.130.4]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBMLttPJ003027 9, 19 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 15:55:55 -0600 9, 19 -- Received: from emlab1 (emlab1.Mines.EDU [138.67.28.173]) 9, 19 -- by inception.Mines.EDU (8.13.1/8.13.1) with ESMTP id mBMLtp0t007782 9, 19 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 14:55:54 -0700 9, 19 -- From: "John Chandler" {jpchandl-at-mines.edu} 9, 19 -- To: {microscopy-at-microscopy.com} 9, 19 -- Subject: Question about indexing diffraction patterns with iTEM software 9, 19 -- Date: Mon, 22 Dec 2008 14:55:50 -0700 9, 19 -- Message-ID: {BC3C7B8AFFAB48789F5AD9E0482F8E55-at-mines.edu} 9, 19 -- MIME-Version: 1.0 9, 19 -- Content-Type: text/plain; 9, 19 -- charset="us-ascii" 9, 19 -- Content-Transfer-Encoding: 7bit 9, 19 -- X-Mailer: Microsoft Office Outlook 11 9, 19 -- Thread-Index: AclkgA2++0VHuClTRrOse5KZjCWGpw== 9, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Thanks to everyone for the excellent suggestions on how to section sand--as well as alternative approaches.
We obtained some chips (not really sections) of an embedded sand grain and I will examine it tomorrow. I'm not too optimistic.
Happy Holidays to everyone!!!! -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: parmiterd-at-mail.nih.gov Name: David Parmiter
Organization: SAIC (Contractor)
Title-Subject: [Filtered] Immunolabeling
Question: Hello, I've a question regarding secondary antibodies for immunolabeling.
Are there specific ones which are best used for a sample embedded in LR-white and using a mouse monoclonal primary? I am planning to use a goat anti-mouse secondary for this, and wanted to see if anyone perhaps had some insight or preferences for specific types that work well. Or perhaps it is mostly the proper concentration that determines the outcome?
The choice for a particular secondary antibody is not much dependent on the type of specimen or embedding. It is different for the primary, however. Some seem to work well with one type of specimen and other primaries again with different types. You may want to choose a secondary that matches your primary (sub)class though for optimum results. I.e. an anti-IgG for a primary IgG. There are mixed anti IgG/IgM available too from several manufacturers, but in general "the tighter the fit" the better.
The concentration....that is a much neglected area. There are also many different ways the desired concentration is established, differing from user to user and not seldom even from experiment to experiment. A general guideline would be to use a concentration between 0.1 and 1 µg/ml which is in accordance with the equilibrium constant for antigen-antibody interactions. One would ideally incubate just long enough to reach equilibrium in binding and to get reproducible results from one experiment to the next. Higher concentrations may give faster results, higher intensity by labelling of low affinity epitopes for instance, but also an increased background risks. Lower concentrations resulting in the opposite pattern. Longer incubation times may result in higher background levels, or when specimens are vulnerable (pre-embedding, cryo utramicrotomy sections) in loss of integrity of the specimen's ultrastructure. It is a matter of balance. The concentrations used are in general lower than the ones applied in fluorescence or "DAB" microscopy as even a low particle background is somehow experienced as more annoying than a similar low background with a fluorescent secondary and/or peroxidase labelled antibodies.....The way we perceive things analogue signals seem to be more forgiving than yes/no particle based systems.
Cheers, Merry Christmas from a very sunny and warm Dunedin (NZ), Hard to get used to a Christmas in the summer season!
Jan Leunissen http://www.aurion.nl
On 23/12/2008, at 4:11 PM, parmiterd-at-mail.nih.gov wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: parmiterd-at-mail.nih.gov } Name: David Parmiter } } Organization: SAIC (Contractor) } } Title-Subject: [Filtered] Immunolabeling } } Question: Hello, I've a question regarding secondary antibodies for } immunolabeling. } } Are there specific ones which are best used for a sample embedded in } LR-white and using a mouse monoclonal primary? I am planning to use } a goat anti-mouse secondary for this, and wanted to see if anyone } perhaps had some insight or preferences for specific types that work } well. Or perhaps it is mostly the proper concentration that } determines the outcome? } } Thanks in advance and Happy Holidays! } } David Parmiter } } Login Host: 129.43.16.230 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Mon Dec 22 21:11:08 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mBN3B7NT005103 } 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 21:11:08 } -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c57607398b03-at-[206.69.208.22]} } 9, 11 -- Date: Mon, 22 Dec 2008 21:11:06 -0600 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Immunolabeling } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 21 -- From leunissen-at-aurion.nl Mon Dec 22 22:36:50 2008 9, 21 -- Received: from mta03.xtra.co.nz (mta03.xtra.co.nz [210.54.141.252]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBN4anJO020260 9, 21 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 22:36:49 -0600 9, 21 -- Received: from [192.168.1.50] (really [122.57.246.53]) by mta03.xtra.co.nz 9, 21 -- with ESMTP 9, 21 -- id {20081223043643.OJFZ26672.mta03.xtra.co.nz-at-[192.168.1.50]} ; 9, 21 -- Tue, 23 Dec 2008 17:36:43 +1300 9, 21 -- Cc: parmiterd-at-mail.nih.gov 9, 21 -- Message-Id: {604DE14A-21CD-4452-90DD-1C1A79067F1E-at-aurion.nl} 9, 21 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200812230311.mBN3BRKk005452-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 9, 21 -- Mime-Version: 1.0 (Apple Message framework v930.3) 9, 21 -- Subject: Re: [Microscopy] viaWWW: Immunolabeling 9, 21 -- Date: Tue, 23 Dec 2008 17:36:44 +1300 9, 21 -- References: {200812230311.mBN3BRKk005452-at-ns.microscopy.com} 9, 21 -- X-Mailer: Apple Mail (2.930.3) 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBN4anJO020260 ==============================End of - Headers==============================
I've cut some hard specimens over the years but never sand. We have a researcher who wishes to look at a section of a sand grain to study the distribution of nanotubes on the surface.
Any suggestions on sectioning a grain of sand? Here's what I am planning:
embedding in hard Spurr resin old, 50 degree diamond knife 2 mm/sec cutting speed
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
As usual Jan gives some excellent advice. I want to clarify one point he made since I routinely run into students and clients who fail to understand a bit of basic immunology. Most commercial secondary antibodies are "anti-IgG (H+L)". The H chain is class specific but the L chain isn't. An anti-IgG (H+L) antibody will also stain IgM and IgA. If you want a class specific antibody, you need to get one made against the Fc portion.
I also agree with Jan's advice of keeping the concentration as low as possible. 70% of the time, I use 1 ug/ml. In about 20% of the time, I find using the primary at 10 ug/ml gives better results and sometimes even need to for the secondary but much less frequently.
Merry Christmas to all of you. Tom
Thomas E. Phillips, Ph.D. Professor of Biological Sciences Chair, MU Faculty Council Director, Molecular Cytology Core 2 Tucker Hall Biological Sciences University of Missouri Columbia, MO 65211-7400 573-882-4712 (voice) 573-882-0123 (fax)
-----Original Message----- X-from: leunissen-at-aurion.nl [mailto:leunissen-at-aurion.nl] Sent: Monday, December 22, 2008 10:38 PM To: Phillips, Thomas E.
Dear David
The choice for a particular secondary antibody is not much dependent on the type of specimen or embedding. It is different for the primary, however. Some seem to work well with one type of specimen and other primaries again with different types. You may want to choose a secondary that matches your primary (sub)class though for optimum results. I.e. an anti-IgG for a primary IgG. There are mixed anti IgG/IgM available too from several manufacturers, but in general "the tighter the fit" the better.
The concentration....that is a much neglected area. There are also many different ways the desired concentration is established, differing from user to user and not seldom even from experiment to experiment. A general guideline would be to use a concentration between 0.1 and 1 µg/ml which is in accordance with the equilibrium constant for antigen-antibody interactions. One would ideally incubate just long enough to reach equilibrium in binding and to get reproducible results from one experiment to the next. Higher concentrations may give faster results, higher intensity by labelling of low affinity epitopes for instance, but also an increased background risks. Lower concentrations resulting in the opposite pattern. Longer incubation times may result in higher background levels, or when specimens are vulnerable (pre-embedding, cryo utramicrotomy sections) in loss of integrity of the specimen's ultrastructure. It is a matter of balance. The concentrations used are in general lower than the ones applied in fluorescence or "DAB" microscopy as even a low particle background is somehow experienced as more annoying than a similar low background with a fluorescent secondary and/or peroxidase labelled antibodies.....The way we perceive things analogue signals seem to be more forgiving than yes/no particle based systems.
Cheers, Merry Christmas from a very sunny and warm Dunedin (NZ), Hard to get used to a Christmas in the summer season!
Jan Leunissen http://www.aurion.nl
On 23/12/2008, at 4:11 PM, parmiterd-at-mail.nih.gov wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: parmiterd-at-mail.nih.gov } Name: David Parmiter } } Organization: SAIC (Contractor) } } Title-Subject: [Filtered] Immunolabeling } } Question: Hello, I've a question regarding secondary antibodies for } immunolabeling. } } Are there specific ones which are best used for a sample embedded in } LR-white and using a mouse monoclonal primary? I am planning to use } a goat anti-mouse secondary for this, and wanted to see if anyone } perhaps had some insight or preferences for specific types that work } well. Or perhaps it is mostly the proper concentration that } determines the outcome? } } Thanks in advance and Happy Holidays! } } David Parmiter } } Login Host: 129.43.16.230 } --------------------------------------------------------------------------- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Mon Dec 22 21:11:08 2008 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id mBN3B7NT005103 } 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 21:11:08 } -0600 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c57607398b03-at-[206.69.208.22]} } 9, 11 -- Date: Mon, 22 Dec 2008 21:11:06 -0600 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: parmiterd-at-mail.nih.gov (by way of MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Immunolabeling } 9, 11 -- Content-Type: text/plain; charset="us-ascii" ; } format="flowed" } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 21 -- From leunissen-at-aurion.nl Mon Dec 22 22:36:50 2008 9, 21 -- Received: from mta03.xtra.co.nz (mta03.xtra.co.nz [210.54.141.252]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBN4anJO020260 9, 21 -- for {microscopy-at-microscopy.com} ; Mon, 22 Dec 2008 22:36:49 -0600 9, 21 -- Received: from [192.168.1.50] (really [122.57.246.53]) by mta03.xtra.co.nz 9, 21 -- with ESMTP 9, 21 -- id {20081223043643.OJFZ26672.mta03.xtra.co.nz-at-[192.168.1.50]} ; 9, 21 -- Tue, 23 Dec 2008 17:36:43 +1300 9, 21 -- Cc: parmiterd-at-mail.nih.gov 9, 21 -- Message-Id: {604DE14A-21CD-4452-90DD-1C1A79067F1E-at-aurion.nl} 9, 21 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- In-Reply-To: {200812230311.mBN3BRKk005452-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed; delsp=yes 9, 21 -- Mime-Version: 1.0 (Apple Message framework v930.3) 9, 21 -- Subject: Re: [Microscopy] viaWWW: Immunolabeling 9, 21 -- Date: Tue, 23 Dec 2008 17:36:44 +1300 9, 21 -- References: {200812230311.mBN3BRKk005452-at-ns.microscopy.com} 9, 21 -- X-Mailer: Apple Mail (2.930.3) 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBN4anJO020260 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 29 -- From PhillipsT-at-missouri.edu Tue Dec 23 07:02:34 2008 23, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 23, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBND2Y8S027712 23, 29 -- for {microscopy-at-microscopy.com} ; Tue, 23 Dec 2008 07:02:34 -0600 23, 29 -- X-IronPort-Anti-Spam-Filtered: true 23, 29 -- X-IronPort-Anti-Spam-Result: ApoEABdwUEnRauUp/2dsb2JhbAC/G1iFBgEBAQeGRIVghRIBAYEv 23, 29 -- Received: from unknown (HELO um-nsmtpout1.um.umsystem.edu) ([209.106.229.41]) 23, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 23 Dec 2008 07:02:33 -0600 23, 29 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 23, 29 -- Tue, 23 Dec 2008 07:02:33 -0600 23, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 23, 29 -- Content-class: urn:content-classes:message 23, 29 -- MIME-Version: 1.0 23, 29 -- Content-Type: text/plain; 23, 29 -- charset="iso-8859-1" 23, 29 -- Subject: RE: [Microscopy] Re: viaWWW: Immunolabeling 23, 29 -- Date: Tue, 23 Dec 2008 07:02:34 -0600 23, 29 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD05A437A1-at-UM-XMAIL06.um.umsystem.edu} 23, 29 -- In-Reply-To: {200812230437.mBN4bark021361-at-ns.microscopy.com} 23, 29 -- X-MS-Has-Attach: 23, 29 -- X-MS-TNEF-Correlator: 23, 29 -- Thread-Topic: [Microscopy] Re: viaWWW: Immunolabeling 23, 29 -- thread-index: AclkuC6HySZDWw5jT5ChzYQ71PJ+aQARam6A 23, 29 -- References: {200812230437.mBN4bark021361-at-ns.microscopy.com} 23, 29 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 23, 29 -- To: {microscopy-at-microscopy.com} 23, 29 -- X-OriginalArrivalTime: 23 Dec 2008 13:02:33.0565 (UTC) FILETIME=[B870F0D0:01C964FE] 23, 29 -- Content-Transfer-Encoding: 8bit 23, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id mBND2Y8S027712 ==============================End of - Headers==============================
Has anyone used the Rowiak LMT F14 laser microtome {www.rowiak.de} ? Are there other laser microtomes on the market? I'd like to know if you are happy with the results. My interest/application is for woody plant material. Thanks and Happy Holidays to everyone! Beth
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
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==============================Original Headers============================== 12, 19 -- From beth-at-plantbio.uga.edu Tue Dec 23 09:17:06 2008 12, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBNFH6C4012887 12, 19 -- for {microscopy-at-microscopy.com} ; Tue, 23 Dec 2008 09:17:06 -0600 12, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 12, 19 -- (authenticated user beth-at-plantbio.uga.edu) 12, 19 -- by dogwood.plantbio.uga.edu 12, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 12, 19 -- for microscopy-at-microscopy.com; 12, 19 -- Tue, 23 Dec 2008 10:17:02 -0500 12, 19 -- Message-Id: {3D5998A5-C5CA-49FD-89DF-FDD328D1B9BB-at-plantbio.uga.edu} 12, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 12, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 12, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 12, 19 -- Content-Transfer-Encoding: 7bit 12, 19 -- Mime-Version: 1.0 (Apple Message framework v929.2) 12, 19 -- Subject: laser microtomes? 12, 19 -- Date: Tue, 23 Dec 2008 10:17:04 -0500 12, 19 -- X-Mailer: Apple Mail (2.929.2) ==============================End of - Headers==============================
In a desperate attempt to find this material, I am asking the list because most of you work in a lab and just may have some sitting around.
I am trying to find Shirley Cotton Strip Assay cloth. It's a roll of cotton cloth about three inches wide with three narrow stripes running the length of it. You bury a piece in the ground for about a month and then measure the tensile strength. This tells you how much microbial activity is happening in the soil. There were literally hundreds of thousands of tests done with this cloth all over the world in the last few decades. Unfortunately the supplier in England stopped making it about a year ago and the supply is now gone.
If anyone coincidentally has any of this they would like to sell please contact me off list.
Thanks,
Tom Kaye
==============================Original Headers============================== 9, 21 -- From tom-at-TomKaye.com Tue Dec 23 10:37:41 2008 9, 21 -- Received: from w2k3-exchfe.mmsasp.local (mx1.techpro.com [66.102.127.13]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id mBNGbfUm029173 9, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 23 Dec 2008 10:37:41 -0600 9, 21 -- Received: from TKdual ([67.134.109.68]) by w2k3-exchfe.mmsasp.local with Microsoft SMTPSVC(6.0.3790.3959); 9, 21 -- Tue, 23 Dec 2008 10:37:40 -0600 9, 21 -- From: "Tom Kaye" {tom-at-TomKaye.com} 9, 21 -- To: {Microscopy-at-microscopy.com} 9, 21 -- Subject: Odd request, looking for Shirley Cotton Strip Assay material 9, 21 -- Date: Tue, 23 Dec 2008 09:37:40 -0700 9, 21 -- Message-ID: {GOEAIJOJDGCJBPIFHOBGKEMPAAAB.tom-at-tomkaye.com} 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; 9, 21 -- charset="iso-8859-1" 9, 21 -- Content-Transfer-Encoding: 7bit 9, 21 -- X-Priority: 3 (Normal) 9, 21 -- X-MSMail-Priority: Normal 9, 21 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) 9, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 9, 21 -- Importance: Normal 9, 21 -- X-OriginalArrivalTime: 23 Dec 2008 16:37:40.0959 (UTC) FILETIME=[C5D8F6F0:01C9651C] ==============================End of - Headers==============================
Keith; You got my imagination working overtime-what were plutonium dioxide particles doing in lung tissue? Was it human lung tissue? How did it get in there? Sounds like there must be a story to this.
John Mardinly, Numonyx
-----Original Message----- X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk] Sent: Tuesday, December 23, 2008 4:38 AM To: MARDINLY, A
I've cut some hard specimens over the years but never sand. We have a researcher who wishes to look at a section of a sand grain to study the distribution of nanotubes on the surface.
Any suggestions on sectioning a grain of sand? Here's what I am planning:
embedding in hard Spurr resin old, 50 degree diamond knife 2 mm/sec cutting speed
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
Ever see the movie, "Silkwood"? If not, take a look. It's based on a true story.
John
} Keith; } You got my imagination working } overtime-what were plutonium dioxide particles } doing in lung tissue? Was it human lung tissue? } How did it get in there? Sounds like there must } be a story to this. } } John Mardinly, } Numonyx } } } -----Original Message----- } X-from: kjmorris-at-well.ox.ac.uk [mailto:kjmorris-at-well.ox.ac.uk] } Sent: Tuesday, December 23, 2008 4:38 AM } To: MARDINLY, A } Subject: [Microscopy] FW: TEM: sectioning sand } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abhigroups-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: I work in the area of DIC microscopy. I want to sample DIC point spread function at any sampling rate specified in theoretical calculation of PSF. The ideal sampling being nyquist rate and need to make sure the shear equals even number of pixels. I like to know procedure and mathematical equations to solve this
Thanks for the reply, Tony. I have had other responses about E1382. One respondent cited ASTM E112 for older methods.
I can pretty easily trash all of the ASTM "standards" using EBSD on modern materials. The ASTM stuff was for classical metallurgical samples. LPCVD and CVD Cu and Al are not the same at all. These grains are small (.2u diameter or larger) and distributed quite differently than bulk metallographic specimens. In some cases, it is impossible to quantify the grains (nodules).
My area of interest is the composition and characteristics of microchip interconnects. These can be .2u wide and be one grain wide. Thus, the grain distribution and intergrannular grain strengths are important factors in the quality of metal interconnects in ICs. IMO, EBSD flies in the face of XRD for real final results. XRD can't produce the facts.
Suppose that I have different data sets that are with 145 total number of grains with 47 edge grains--145:47. Then, compare this to 1915:93. I get very similar results.
You quote ASTM E1382. (The older manual methods are discussed in ASTM E112. But these methods are obsolete for the smaller grains that LPCVD, CVD, etc. produce. The gross grains are at about 200X. The finer grains are at 50KX or greater.
Thus, I search and study for for a quantitative method of grain analysis for modern day IC interconnects.
gary g.
At 09:06 PM 12/26/2008, you wrote: } Gary: } } Get a copy of: } } ASTM, E1382-97, Determining Average Grain Size Using Semiautomatic and Auto } Image Anal, 2004. } } and } } ASTM, E112-96, Determining Average Grain Size, 2004 } } } 400-600 grains is reasonably for {=10% precision, same as point counting. } } } } Tony } } } ...................................................................... } Andrew Anthony "Tony" Havics, CHMM, CIH, PE } pH2, LLC } 5250 E US 36, Suite 830 } Avon, IN 46123 } www.ph2llc.com } } (317) 718-7020 off } (317) 718-7038 fax } (317) 409-3238 cell } } 90% of Risk Management is knowing where to place the decimal point...any } consultant can give you the other 10%(SM) } } This message is from pH2. This message and any attachments may contain } legally privileged or confidential information, and are intended only for } the individual or entity identified above as the addressee. If you are not } the addressee, or if this message has been addressed to you in error, you } are not authorized to read, copy, or distribute this message and any } attachments, and we ask that you please delete this message and attachments } (including all copies) and notify the sender by return e-mail or by phone at } 317-718-7020. Delivery of this message and any attachments to any person } other than the intended recipient(s) is not intended in any way to waive } confidentiality or a privilege. All personal messages express views only of } the sender, which are not to be attributed to pH2 and may not be copied or } distributed without this statement. } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Sunday, December 21, 2008 5:38 PM } To: ph2-at-sprynet.com } Subject: [Microscopy] EBSD data-statistically significant } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 21 -- From gary-at-gaugler.com Sat Dec 27 00:10:34 2008 13, 21 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBR6AX6M004263 13, 21 -- for {microscopy-at-microscopy.com} ; Sat, 27 Dec 2008 00:10:34 -0600 13, 21 -- Message-Id: {200812270610.mBR6AX6M004263-at-ns.microscopy.com} 13, 21 -- Received: (qmail 5997 invoked from network); 26 Dec 2008 22:09:19 -0800 13, 21 -- Received: by simscan 1.1.0 ppid: 5991, pid: 5992, t: 0.1459s 13, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 13, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 13, 21 -- by smtp1 with SMTP; 26 Dec 2008 22:09:19 -0800 13, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 21 -- Date: Fri, 26 Dec 2008 22:10:25 -0800 13, 21 -- To: "Tony Havics, CHMM, CIH, PE" {aahavics-at-ph2llc.com} 13, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 13, 21 -- Subject: RE: [Microscopy] EBSD data-statistically significant 13, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 13, 21 -- In-Reply-To: {20081227050728.BB8262E033-at-mx2.calweb.com} 13, 21 -- References: {200812212237.mBLMbeT6010959-at-ns.microscopy.com} 13, 21 -- {20081227050728.BB8262E033-at-mx2.calweb.com} 13, 21 -- Mime-Version: 1.0 13, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Still operating in the film days (not too much longer, we hope!). Anyone out there still selling Kodak 5302 Fine-grain release positive in 100' rolls? I'm down to my last one, and Tmax100 is a bit too fast and too grainy.. Julian
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733
The old ASTM standards are not what I seek. Perhaps when ASTM gets done with what they started in 2003, that might be of help. But I can't wait another five years to have a workable approach to analyzing IC metal.
Quoting from WK3577:
"This guide covers the measurement of ASTM G and other measures of grain size by electron backscatter diffraction (EBSD) mapping. It covers specimen preparation and instrument setup for EBSD analysis. It also provides guidance on the definition of a grain for purposes of analysis by EBSD. EBSD is a direct method of measuring grain size. It will be used on materials that are resistant to the standard method of grain boundary etching and measurement in the light optical microscope."
Since EBSD indexing is based on the Hough transform, and the beam is linearly scanned across and down the specimen, the sampling is not random--other than deciding where on the specimen to set the scan area. If you are not familiar with automated EBSD, see if you can find some background data (there are several Web sources) and see what you think.
Here is a typical scenario:
Bond pad is Al and is either rectangular or square. It could be M1 or M2 or M2 on M1. Grain size (average diameter) might range from 0.15u to 0.3u. For a pad of about 70u x 70u, what area value should be scanned at what step size and what mag? The scan area is a rectangle or square--not a circle. For each scan and associated parameters, one finds that there are a certain number of good points, number of grains and number of edge grains (edge grains are typically excluded from all calculations).
With relatively small grains, the grain boundaries become significant. EBSD allows the operator to essentially define what is a grain. Usually this is the number of degrees of basal plane orientation between adjacent grains. Typical value is five degrees.
For the pads I am currently studying, 4KX mag with scan area of 9u x 12u and step size of 0.05u or 0.06u works well and is repeatable.
gary g.
At 11:06 PM 12/26/2008, you wrote: } 1. For # grains per area } } The stats are that same as in the ASTM methods (transect and pt coint). } This is because they are based point counting stats. Chayes' addressed this } (NIST of the 1960s) in looking at the basis for point counting (grain } counting) [I have his book from that time period addressing the probability } and statistics aspects). Provided the sampling is regionally and locally } random, the data points should follow a poisson distribution with the } variation proportional to the numbers of counts (grains). The higher the } number of grains, the lower the Coefficient of Variation (CV). We use this } same basis for estimating variability in fiber counts on filters looking to } get a CV { 0.15. } } 2. Grain size distribution } } The grain distribution is another aspect and the question is really one of } geo-statistical variability (Geostatistics deals with spatially } autocorrelated data). The best way to assess this would be using a } statistical distribution of nearest like points by distance, called a } semi-variogram (these can also be related to fractal dimensions). One can } also investigate anisotropy using a directionally-based estimator. A second } way to do this is to look at clustering factors, of which there are a few. } } 3. Grain distribution and frequency can also be assessed looking at } power spectrums. } } } } ...................................................................... } Andrew Anthony "Tony" Havics, CHMM, CIH, PE } pH2, LLC } 5250 E US 36, Suite 830 } Avon, IN 46123 } www.ph2llc.com } } (317) 718-7020 off } (317) 718-7038 fax } (317) 409-3238 cell } } 90% of Risk Management is knowing where to place the decimal point...any } consultant can give you the other 10%(SM) } } This message is from pH2. This message and any attachments may contain } legally privileged or confidential information, and are intended only for } the individual or entity identified above as the addressee. If you are not } the addressee, or if this message has been addressed to you in error, you } are not authorized to read, copy, or distribute this message and any } attachments, and we ask that you please delete this message and attachments } (including all copies) and notify the sender by return e-mail or by phone at } 317-718-7020. Delivery of this message and any attachments to any person } other than the intended recipient(s) is not intended in any way to waive } confidentiality or a privilege. All personal messages express views only of } the sender, which are not to be attributed to pH2 and may not be copied or } distributed without this statement. } } } -----Original Message----- } From: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } Sent: Saturday, December 27, 2008 1:15 AM } To: ph2-at-sprynet.com } Subject: [Microscopy] RE: EBSD data-statistically significant } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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I don't have these. If they will help, I can get them. Thanks for the leads.
The point scans are to determine grain boundaries. This is where it gets dicey based on the scan parameters. For your 1), I'm not sure that pixel size has any bearing on the results. The camera binning changes frames per second but not necessarily accuracy. I'm using 8x8 binning and run at between 22fps and 45fps.
As for 2), I don't see the relevance since the data is actually conveyed as photons on the EBSD camera phosphor disc.
This:
} } Yours appears to be more of a probability question of is it real? And with } } what level of confidence? is the real crux of the problem/situation. It is easy to generate numbers...but are they valid? If so or not so, how to know for sure? I think I'm converging on this based on twenty scans of the same area so far. But of course, this is just one portion of one specimen. A lot of variability reduction is needed for me to say a more definitive description of a data collection procedure.
gary g.
At 03:02 PM 12/27/2008, you wrote: } Gary: } } Do you have: } } 1. Day, A comparison of grain imaging and measurement using horizontal } orientation and colour orientation contrast, BSED and optical, J Microscopy, } 195, 3, 186-196, 1999 } } 2. Humphreys, Electron backscatter diffraction of grain and subgrain, } resolution considerations, J Microscopy, 195, 3, 212-216, 1999 } } Especially Humphreys p 214 } } 3. Humphreys, Quantitative metallography by electron backscattered } diffraction, J Microscopy, 195, 3, 170-185, 1999 } } Especially Humphreys p 179-180. } } } } } The basic counting aspects still go back to the original stats of interceot } and point counting. } } Your problem appears to be 1) the relative size of the grain compared to the } pixel size in an image and 2) to the scan rate regarding pickup of electrons } to avoid aliasing or modulated effects; these could be analyzed if you take } a scan sequence isub1 to isubn values and perform a fourier analysis to see } if there is a time delayed effect; another and perhaps better way might be } to do a laplace transform on the values isubn+1 minus isubn as t is now in } an easy domain to see if there is a shift. As the grain size approaches the } pixel size the error will increase significantly (but you knew that } already). } } Yours appears to be more of a probability question of is it real? And with } what level of confidence? } } } } } ...................................................................... } Andrew Anthony "Tony" Havics, CHMM, CIH, PE } pH2, LLC } 5250 E US 36, Suite 830 } Avon, IN 46123 } www.ph2llc.com } } (317) 718-7020 off } (317) 718-7038 fax } (317) 409-3238 cell }
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Question: Is it possible to do optical sectioning of Arabidopsis seeds with confocal microscopy? say, if I remove the seed coat, which I understand would be quiet opaque, would it work then?
You can see through a couple of layers of the intact embryo inside, which I guess is what you want to do. If you fix in paraformaldehyde first, you can dissect out the embryo without influencing the distribution of GFP too much, if that's what you want to do. If you fix, you can then bisect the embryos longitudinally (easiest with a sapphire knife) and also see distribution of GFP-tagged cells/organelles, or do immunolocalisation.
And if you clear the tissue, you can section right through the embryo, shown very beautifully in this paper: http://www.brookes.ac.uk/lifesci/runions/pdfs/Truernit%20et%20al%20Plant%20C ell%202008.pdf! - Truernit et al. 2008 Plant Cell online . cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 30/12/08 12:58 AM, "vivir-at-psb.ugent.be" {vivir-at-psb.ugent.be} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both vivir-at-psb.ugent.be as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: vivir-at-psb.ugent.be } Name: Vikram } } Title-Subject: [Filtered] Seed confocal microscopy } } Question: Is it possible to do optical sectioning of Arabidopsis } seeds with confocal microscopy? say, if I remove the seed coat, which } I understand would be quiet opaque, would it work then? } } Login Host: 157.193.250.13 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 5, 11 -- From zaluzec-at-microscopy.com Mon Dec 29 07:50:39 2008 } 5, 11 -- Received: from [206.69.208.22] (msdvpn8.msd.anl.gov [130.202.238.72]) } 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mBTDobx5017320 } 5, 11 -- for {microscopy-at-microscopy.com} ; Mon, 29 Dec 2008 07:50:38 -0600 } 5, 11 -- Mime-Version: 1.0 } 5, 11 -- Message-Id: {p06240800c57e861d06f5-at-[206.69.208.22]} } 5, 11 -- Date: Mon, 29 Dec 2008 07:50:36 -0600 } 5, 11 -- To: microscopy-at-microscopy.com } 5, 11 -- From: vivir-at-psb.ugent.be (by way of MicroscopyListserver) } 5, 11 -- Subject: viaWWW: Seed confocal microscopy } 5, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
I'd like to tap collective wisdom of those members of listserver who do decapsulation and sample preparation of integrated circuits for emission or IR microscopy.
What is your opinion on CNC sample preparation systems, a.k.a. "Chip-Unzip"? I have pretty good idea of what and how this sample preparation tool is supposed to do, but would be interested to know what are the real-world limitations, hurdles, and which "gotcha" should be expected while trying to decapsulate devices or thin and polish substrates.
I could post a summary of the replies, if there is interest in this subject.
Valery Ray
============================ www.partbeamsystech.com PBS&T, MEO Engineering Co, Inc. 290 Broadway St., Suite 298 Methuen, MA 01844 Phone: (978) 296-5063
==============================Original Headers============================== 8, 23 -- From vray-at-partbeamsystech.com Tue Dec 30 11:36:29 2008 8, 23 -- Received: from smtp103.biz.mail.re2.yahoo.com (smtp103.biz.mail.re2.yahoo.com [68.142.229.217]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id mBUHaSgH022950 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 30 Dec 2008 11:36:28 -0600 8, 23 -- Message-Id: {200812301736.mBUHaSgH022950-at-ns.microscopy.com} 8, 23 -- Received: (qmail 86273 invoked from network); 30 Dec 2008 17:36:28 -0000 8, 23 -- Received: from unknown (HELO cp1198275a) (vray-at-75.68.111.131 with login) 8, 23 -- by smtp103.biz.mail.re2.yahoo.com with SMTP; 30 Dec 2008 17:36:27 -0000 8, 23 -- X-YMail-OSG: c03oGRkVM1k1G.MBPOKt1G8ztty4U0ryR7NrX1KTgOq9C3k6MhbBZOBRJRgjB55kK5s0CKy8fEDKpCaIBLWSDsXVt97YD5jDHHMrS8SYLe5NlPbc7gJIkmF9zEBNKHQuIlnV50YIiU3spsnJ.4TLyS_mJO1Z42gKAC3hbdD.g05u.Ar1.x4yEiZ9z5Xqix8KNINKKPtBozpbxeIph.gI5ngHyBctCBPLPxDI5Th3UoKbHJ6oNADFkeeNRw-- 8, 23 -- X-Yahoo-Newman-Property: ymail-3 8, 23 -- Reply-To: {vray-at-partbeamsystech.com} 8, 23 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- Subject: Decapsualtion and backside sample preparation of semiconductors. 8, 23 -- Date: Tue, 30 Dec 2008 12:39:12 -0500 8, 23 -- Organization: PBST / MEO Engineering 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 8, 23 -- Thread-Index: Aclqo1e5Z9M0+1I6TDqdYvMSCueyjAAAcgVw 8, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] Stains for proteins
Question: I have a new project which may entail staining proteins so that there is more contrast for TEM imaging. One collaborator recommended osmium tetroxide, but I read on the web that it is rather poisonous and requires special handling. I have not done this sort of biological TEM before and I would appreciate any tips or references.
It's been a few years since I've been on the list server - doing those recommended postdocs to "broaden my horizons" beyond microscopy (I'm wondering if I should have followed that advice!).
I'm gearing up to teach an EM class for biologists to undergrads. The class hasn't been taught at my university in eons, so I'm basically starting from scratch with a great set of brand new JEOL scopes and a lab full of virgin equipment. It would be a dream come true if not for the fact that I've got to learn how to use all of this brand new equipment in short order!
I'm sure that I'll be in touch throughout the upcoming semester, but for now, I have a few questions on which I'd like your learned advice:
1. Does anyone have experience with a JEOL JEM1011 TEM? Thoughts on it? Do you have an abbreviated user's protocol that you would be willing to share? No one here has ever used this brand new (though 5 year old scope), and, though JEOL will graciously come and do a training session with me, I'd love any input you may have.
2. Going digital. We are looking at getting a digital image capture system. I'm old enough to have been trained in the darkroom long ago. Should I teach these students darkroom technique or just assume that they're in the digital age and go with either digital capture or digital image manipulation (scanning in EM negatives and printing)? Vote and let me know. And, if you've got a camera system, scanner or printer that you would recommend, that would be great.
3. Lastly, and this may show the old workhorse microscopes I was weaned on - LN2. I've been advised to just forget about using LN2 with this TEM for biological applications. Really? Granted, I'll talk to the JEOL rep about this, but if you've got thoughts on it, please share. I hear that getting LN2 into our lab may be a problem, but my gut tells me that I should make ripples with this one.
4. Okay, one more, but it's an easy one. Does anyone have a recommendation for a quick and easy animal tissue to use for teaching? I'm a die hard plant person (perfusion - ahh!), but I think that not having experience handling and looking at animal tissue has been a handicap for me. I don't want that for my students. Can I just go pick up some chicken livers or something and not have to find something to sacrifice?
That's it for now. I know that there will be more.
Many thanks, Kristen
Kristen A. Lennon, Ph.D. Lecturer, Department of Biology 202 Compton Science Center Frostburg State University 101 Braddock Road Frostburg, MD 21532 k.lennon-at-frostburg.edu
==============================Original Headers============================== 13, 20 -- From kamlennon-at-yahoo.com Wed Dec 31 18:30:31 2008 13, 20 -- Received: from web84007.mail.mud.yahoo.com (web84007.mail.mud.yahoo.com [68.142.206.177]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id n010UVQW011058 13, 20 -- for {Microscopy-at-Microscopy.com} ; Wed, 31 Dec 2008 18:30:31 -0600 13, 20 -- Received: (qmail 23609 invoked by uid 60001); 1 Jan 2009 00:30:30 -0000 13, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 20 -- s=s1024; d=yahoo.com; 13, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Message-ID; 13, 20 -- b=CqDVTUJAqH9OmwKIT05TcFhilN8W5b4TH4VVVw3XGiIuPorbXVxCisHm0it7iPSLaS9sjsY/LhmeJNa3JpJo/lOiIKMqBLserDqmQ1+3yFel3/IfzViKtTyyx4AUo6roq5dZ4iDp7nUrjnWr7aoXqkXozPM4YrBTri8ScUff9eU=; 13, 20 -- X-YMail-OSG: KuV9JQgVM1kqFtyeJQ5xhd81MO9MDtVbiXwLAuz7_j.epu5Np3DdPvW8kKX1n_x9ZA-- 13, 20 -- Received: from [96.239.149.81] by web84007.mail.mud.yahoo.com via HTTP; Wed, 31 Dec 2008 16:30:30 PST 13, 20 -- X-Mailer: YahooMailWebService/0.7.247.3 13, 20 -- Date: Wed, 31 Dec 2008 16:30:30 -0800 (PST) 13, 20 -- From: Kristen Lennon {kamlennon-at-yahoo.com} 13, 20 -- Reply-To: kamlennon-at-yahoo.com 13, 20 -- Subject: Developing EM class for undergrads + going digital advice? 13, 20 -- To: Microscopy-at-Microscopy.com 13, 20 -- MIME-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=us-ascii 13, 20 -- Message-ID: {295847.22686.qm-at-web84007.mail.mud.yahoo.com} ==============================End of - Headers==============================
One stain is tannic acid/ferric chloride, it's a good alternative to osmium. I've usually used this for membranes, but it does stain things like cytoskeletal proteins and ribosomes pretty well too - it probably binds to certain charged surfaces or groups, like most fixatives.
You can do this a number of ways, the two commonest ones are to either fix first in aldehyde (glutaraldehyde or formaldehyde) in appropriate buffer, rinse well, then stain in 1% tannic acid, rinse well, then stain in 1% ferric chloride. The tissue will go black, just as if it has been osmicated. Then continue as usual - dehydrate, embed.
The alternative is to add the tannic acid in with the aldehyde fixative, which is the way I've usually used it for plant tissues.
The ferric chloride is just made up in distilled water, and you can vary the concentration of either TA or FeCl3, and vary the time, etc.
I imagine you are fixing animal/human tissue (I work on plants only), so just go with whatever is the standard TEM protocol and add the TA/FeCl3 as above. It used to be the case that for reliable results you had to use Mallinkrodt tannic acid, but the manufacturing process used by other companies may be better now.
There are a couple of older references to this which I can dig out - the paper we cite them in is pre-web.....
cheers, from 2009 already downunder... Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
ph 61 2 6246 5475 fx 61 2 6246 5334
On 1/01/09 7:46 AM, "twigg-at-estd.nrl.navy.mil" {twigg-at-estd.nrl.navy.mil} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both twigg-at-estd.nrl.navy.mil as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: twigg-at-estd.nrl.navy.mil } Name: Mark Twigg } } Organization: Naval Research Laboratory } } Title-Subject: [Filtered] Stains for proteins } } Question: I have a new project which may entail staining proteins so } that there is more contrast for TEM imaging. One collaborator } recommended osmium tetroxide, but I read on the web that it is rather } poisonous and requires special handling. I have not done this sort } of biological TEM before and I would appreciate any tips or } references. } } Thanks, } } Mark } } Login Host: 132.250.134.121 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Wed Dec 31 14:38:46 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } mBVKcjqv024150 } 8, 11 -- for {microscopy-at-microscopy.com} ; Wed, 31 Dec 2008 14:38:45 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240800c58188c4a985-at-[206.69.208.22]} } 8, 11 -- Date: Wed, 31 Dec 2008 14:38:45 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: twigg-at-estd.nrl.navy.mil (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: Stains for proteins } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================