Over the Christmas break we routinely shut our microscopes down to run only on ion pumps as our cooling water can be a little "temperamental".
This year we had a power glitch which turned the microscopes completely off. To top it off our cooling water is out of action for maintenance until next week!! I was hoping to get all the ion pumps running on the microscopes to keep the vacuums at leat a bit clean and I have managed to get this done on all but our JEOL 2010. Does anyone know if it is possible to get just the ion pumps running without needing cooling water on a 2010?
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==============================Original Headers============================== 17, 28 -- From Colin.Veitch-at-csiro.au Mon Jan 2 18:38:05 2006 17, 28 -- Received: from vic-ironport-ext-out1.csiro.au (vic-ironport-ext-out1.csiro.au [150.229.64.37]) 17, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k030c3V4024434 17, 28 -- for {microscopy-at-msa.microscopy.com} ; Mon, 2 Jan 2006 18:38:04 -0600 17, 28 -- DomainKey-Signature: s=email; d=csiro.au; c=nofws; q=dns; b=xU3RCN8AXAmWEvu85jV1wrWTuUHnFiSlBXAaGGnTue1S/WJESDcqcwHjBKBfIWsOg4hoNydsUlK2UaXg0lctHoNuIRakZsSu0pTgT/UHQM1uofzGf4egwYNOnYE3LgVO; 17, 28 -- Received: from exgw1-mel.nexus.csiro.au ([138.194.3.56]) 17, 28 -- by vic-ironport-ext-out1.csiro.au with ESMTP; 03 Jan 2006 11:38:02 +1100 17, 28 -- X-IronPort-AV: i="3.99,323,1131282000"; 17, 28 -- d="scan'208"; a="63973513:sNHT23878160" 17, 28 -- Received: from exvic5-gex.nexus.csiro.au ([138.194.194.6]) by exgw1-mel.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 17, 28 -- Tue, 3 Jan 2006 11:38:02 +1100 17, 28 -- content-class: urn:content-classes:message 17, 28 -- MIME-Version: 1.0 17, 28 -- Content-Type: text/plain; 17, 28 -- charset="us-ascii" 17, 28 -- Subject: Restarting a JEOL 2010 on ion pumps 17, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 17, 28 -- Date: Tue, 3 Jan 2006 11:38:02 +1100 17, 28 -- Message-ID: {32CDDDAA7161394599F0025494915749051DD3-at-exvic5-gex.nexus.csiro.au} 17, 28 -- X-MS-Has-Attach: 17, 28 -- X-MS-TNEF-Correlator: 17, 28 -- Thread-Topic: Restarting a JEOL 2010 on ion pumps 17, 28 -- Thread-Index: AcYP/fQ3/LdcT+y2Rj221MWzoKvg+g== 17, 28 -- From: {Colin.Veitch-at-csiro.au} 17, 28 -- To: {microscopy-at-msa.microscopy.com} 17, 28 -- X-OriginalArrivalTime: 03 Jan 2006 00:38:02.0959 (UTC) FILETIME=[F47CA5F0:01C60FFD] 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k030c3V4024434 ==============================End of - Headers==============================
FOCUS ON MICROSCOPY 2006, Perth, Australia, April 9-12, 2006 19th International Conference on 3D Image Processing in Microscopy 18th International Conference on Confocal Microscopy
Dear Colleagues
January 9, 2006, the deadline for abstract submission for the Perth conference is nearing.
Please submit your abstract by that date.
The program for the conference will be finalized and available on our website on Jan 23, 2006. Authors will also be informed individually by E-mail on the placement of their contribution in the program. Abstracts for oral and poster presentations are sollicited. Submission preferably through the conference website: http://focusonmicroscopy.org/ where also the conference registration is open and hotel booking information is available.
The earlier than usual deadline of 9 Jan. was chosen to give delegates sufficient time -after the program has been announced- to arrange their travel and acccommodation before the conference starting date of Sunday 9 April, 2006.
Please note that the conference will take place in the Esplanade Hotel in Fremantle, the historic waterfront suburb of Perth, See further our website.
The program will start on Sunday April 9, around 18 hours with an opening symposium in the Esplanade hotel followed by a welcome reception.
The conference Focuson Microscopy 2006 will take place as the next in a series of unique interdisciplinary meetings on advanced multidimensional light microscopy and image processing. The conference will be hosted by the University of Western Australia in Perth.
Focus on Microscopy 2006 is the continuation of a conference series presenting the latest innovations in optical microscopy and its applications in biology, medicine, material science, and information storage. 3D optical imaging and related theory are traditional subjects for the conference. The conference series is also known for covering the rapid development of advanced fluorescence labeling techniques for the confocal and multi-photon 3D imaging of -live- biological specimens. This year, in addition, special attention will be given to imaging in thick tissues.
Abstracts for contributions are invited and can now be submitted through the website:
www.FocusOnMicroscopy.org
where further information on the present and previous FOM conferences can be found.
Important dates:
Deadline for the submission of abstracts: January 9, 2006 Draft program available on the web: January 23, 2006 at website www.FocusOnMicroscopy.org Deadline for early registration: February 20, 2006
Welcoming you to beautiful Perth for the FOM2006 conference and exhibition.
With the best wishes for the New Year and on behalf of the organizing committee,
David Sampson, University of Western Australia, Perth, Australia
Fred Brakenhoff Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands
E-mail: info2006-at-FocusOnMicroscopy.org
Web: www.FocusOnMicroscopy.org
==============================Original Headers============================== 39, 30 -- From brakenho-at-science.uva.nl Tue Jan 3 02:18:50 2006 39, 30 -- Received: from imap.science.uva.nl (imap.science.uva.nl [146.50.4.51]) 39, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k038IluR006028 39, 30 -- for {microscopy-at-msa.microscopy.com} ; Tue, 3 Jan 2006 02:18:48 -0600 39, 30 -- Received: from webmail.science.uva.nl [146.50.4.91] 39, 30 -- by imap.science.uva.nl with ESMTP (sendmail 8.11.6p2/config 11.36). 39, 30 -- id k038Ij231650; Tue, 3 Jan 2006 09:18:46 +0100 39, 30 -- Received: from localhost 39, 30 -- by webmail.science.uva.nl (sendmail 8.11.6/config 11.35). 39, 30 -- id k038Ij232296; Tue, 3 Jan 2006 09:18:45 +0100 39, 30 -- X-Organisation: Faculty of Science, University of Amsterdam, The Netherlands 39, 30 -- X-URL: http://www.science.uva.nl/ 39, 30 -- Received: from localhost ([127.0.0.1]) 39, 30 -- (SquirrelMail authenticated user brakenho); 39, 30 -- by webmail.science.uva.nl with HTTP; 39, 30 -- Tue, 3 Jan 2006 09:18:45 +0100 (CET) 39, 30 -- Message-ID: {51496.84.254.54.91.1136276325.squirrel-at-84.254.54.91} 39, 30 -- Date: Tue, 3 Jan 2006 09:18:45 +0100 (CET) 39, 30 -- Subject: FOM2006 abstract deadline 9 Jan. near! FocusOnMicroscopy, Perth, 39, 30 -- April 9-14 2006 39, 30 -- From: "Fred Brakenhoff" {brakenho-at-science.uva.nl} 39, 30 -- To: microscopy-at-msa.microscopy.com 39, 30 -- User-Agent: SquirrelMail/1.4.3a 39, 30 -- X-Mailer: SquirrelMail/1.4.3a 39, 30 -- MIME-Version: 1.0 39, 30 -- Content-Type: text/plain;charset=iso-8859-1 39, 30 -- Content-Transfer-Encoding: 8bit 39, 30 -- X-Priority: 3 (Normal) 39, 30 -- Importance: Normal 39, 30 -- X-Virus-Scanned: by amavisd-new ==============================End of - Headers==============================
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Email: fisiltd-at-gmail.com Name: Howard Freeman
Organization: FISI Ltd
Title-Subject: [Filtered] O'Brien & McCully, Study of Plant Structure
Question: I have a copy of The study of Plant Structure, O'Brien & McCully, first printing, 1981 in very good condition. I used this for my teaching but have moved away from histology. If anyone is interested please send email to fisiltd-at-gmail.com
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Organization: pos-doc at INSA - Institut National des Sciences AppliquČes de Lyon
Title-Subject: [Filtered] LM on 304L grey superficial layer and black lines
Question: I have shot peening 304L steel specimens, thickness about 5mm. Objective to visualise martensitic phase. I used tradional mechanical polishing, till 1200 grade, different etching based on water, HCl and potassium dissulfite (Berahas's tint). I did not clearly observe a martensitic layer but some insland, there is a dark grey to black layer on treated surface of about 10 microns, in case of highest resolution 200x I see it bright grey. What you believe it to be? I observe black flowing lines, variable lengths from 5 to 200 microns , paralel to surface through all specimen thickness and all its length. What it cab be? Some mention they are mechanical polishing effect others ferrite phase.
Thanks for whom took time reading or answering! Best new year wishes! Soraia Pirfo
Hi Sue, You give me an occasion to offer via the listserver some lore on MnO4 staining and epoxy resin adaptations to MnO4 staining that I provided off-listserver earlier this year in 2 other messages. I'll offer my message in three lengths.
SHORT VERSION is: Using epoxy embedding that excludes MNA (MNA?), MnO4 section staining followed by a lead stain (Sato's) can give fantastic contrast to everything --- not just membranes --- including any dirt/oil collected from the trough when picking up the sections, or rinsed off the pipette tip when dispensing drops of stain. The contrast is even greater if tannic acid has been used in the fix. Section staining of Epon-DDSA or Spurr embeddings is OK, but Araldite gives distinctly finer-grain higher-resolution staining.
MEDIUM VERSION: NMA renders use of KMnO4 as a section stain impossible; MnO4 reacts with the cured resin. Reedy, J Cell Biol 1965 26:309-11
We prefer the Mn-Pb sequence for higher contrast generally, and especially in the thinnest sections - 15-30 nm. So we gave up NMA about 30 years ago, and just heretically fiddled the resin-DDSA proportions until we got a hardness we liked; it worked just fine. With Epon that was that. We prefer Araldite because it is less even reactive than Epon or Spurr with MnO4. With Araldite-DDSA, we use Araldite 506. We found the best mixture still a bit too brittle, so added a bit of DER 736 as a "plasticizer", have used that for over 30 years. Araldite 506 50g 10g DDSA 75g 15g DER 736 10g 2g 1.8% v/v DMP-30 (range 1.5% to 2.0%). Reference Reedy et al (1983) J. Muscle Res. Cell Motil. 4:25-53.
LONG LONG VERSION (data dump): (This comes mostly from my off-listserver reply to some contributors to NetNotes in the May or June 2005 Microscopy today, regarding a query by Ursula J Potter about MnO4 use as a section stain.)
I have used 1-2% MnO4 section staining since 1964, almost exclusively since 1970, always followed by Pal's bleach (see below) to eliminate surface "pepper" of MnO2 ppt, followed then by a lead stain, of which Sato's appeals to us as the best and most storage-stable (Hanaichi et al, 1986, Proc 11th Internat'l EM Congress (Kyoto), p 2181). After publishing my brief note on the MNA problem (J Cell Biol, 1965, 26:309), I soon switched completely to Araldite-DDSA embedding, because it turned out that cured Araldite was much less reactive with MnO4 than any other epoxy resin mix I ever tested, including Spurr's when it later appeared. I used an extreme TEST, exposing cured blocks of various embedding resin mixtures to HOT KMnO4 solution (test tube immersed in boiling water) for 1 hr. In that test, the Epon-DDSA resin "preferred" in my Brief Note developed a blackened micro-fissured tree-bark surface (like that of MNA-Epon in unheated KMnO4, while the Araldite-DDSA resin was scarcely discolored at all. The Epon-DDSA sections typically presented a more nano-granular staining than did Araldite sections.
I think I gave up using barium MnO4 because it seemed to give blotchy staining more often than KMnO4. I also guessed that barium ion might capture carbonate as Pb did; Pb carbonate was the ubiquitous contaminant from hell with all the early lead stains that preceded lead citrate.
We wanted the maximum staining contrast that is provided by the Mn-Pb sequence because we mainly use 15-30 nm sections. Adding in UrAc to the sequence helps not at all. We do use UrAc as a block-stain, or as a secondary post-fixative (preferred to OsO4 in my lab) when our primary fixative is tannic acid with or (usually) without glutaraldehyde (TA-URAC works beautifully on myofilaments and on residual membranes in our permeabilized demembranated insect muscle, both in aqueous buffers at room temperature and as freeze-subst'n chemistry in acetone at -80 to -90°C). Recently, we have even found that the actin monomer substructure of thin myofilaments can be seen-- it appears to be relatively negatively stained, coated with metal derived from the TAURAC and Mn-Pb sequences (Fig 3, blue outlined: Fig 5 B; Liu et al, J Struct Biol, 2004, 147:268-282).
We had to experiment to find the optimum staining times for KMnO4, Pal's bleach washing, and lead stain needed to saturate contrast without "digesting" or extracting structures out of the sections. We learned by deliberately over-treating sections with each step. 60-90 min will remove Z-bands and (maybe) myofilaments from 100-150 nm Araldite sections, leaving a negative image, actually a cavity in the resin. Staining saturatees slower in Araldite than in Epon sections. 100 nm sections become stain-saturated slower than 25 nm sections. Cross-sections of muscle saturate much faster than longitudinal sections. 2-10 min in MnO4, 10-30s washing in Pal's bleach 1:100, and 1-5 min on Sato's Pb stain works for 25 nm longitudinal sections of muscle.
Stain apparently penetrates the section only where the pure embedding medium is modified by the presence of stained structures that intercept the section surface. The stains percolate only through the embedded structures, NOT through blank empty resin regions, as we found when sections had piled up or folded two-deep on one another; the covered section (sandwiched between carbon support film and an overlying surface section) would appear stained only within 0.1-0.2 um laterally from wherever the overlying section contained embedded structures such as filaments or membranes to conduct stain from the solution to the underlying structures in a covered section.
PAL's BLEACH, from the J. D. Robertson 1963 paper cited in my 1965 brief note: Distilled water 100 ml Potassium sulphite 0.5 g Oxalic acid 0.5 g dilute 1:100 for use (3-4 drops of above stock in 10 ml water), and run 10-20 drops over the grid after MnO4 staining. Water wash immediately after MnO4 and after Pal's.
All our staining is done by floating grids on small spherical drops of stain on fresh-washed Parafilm. (I'm not familiar with the vertical-insertion immersion staining of grids mentioned by Dr W. Muss in his helpful reply to you Dec 30.) Keys to clean staining: draw up the stains from under the surface in the stock bottles, discard the first 3-6 drops before putting the drop to be used down on a freshly washed Parafilm surface. Mistrust paranoically all unwashed surfaces on forceps and parafilm and pipette barrels as likely to have finger grease that will get on your sections or support films and become intensely stained contaminants. Wash everything you mistrust that will contact sections and grids with lavish flows and jets of deionized or distilled water. Stock lead stain needs replacing when it starts to "fails" in producing contrast, as often as every 4 weeks. Stock 2% KMnO4 lasts "forever"; just remember to respect and go below its MnO2-dirty surface when filling your pipette.
Around 1970 I switched to a lower-viscosity Araldite 506 mix (with DDSA, DER 732, DMP-30: see MEDIUM VERSION above for recipe and reference) that we have used ever since. It becomes as water-thin in viscosity as Spurr's when heated to 60°C, so we rotate vials on a small tilted platform in the oven for 20' x 3 changes to optimize infiltration, bypassing the 50:50 (etc) intermediate solvent-resin mixtures to go directly from solvent into 100% accelerated resin mix. We soon abandoned some of the other old-time religion as well, giving up propylene oxide completely to go directly from acetone or ethanol, after we tried deliberately curing 10-15 ml vials of accelerated resin with 10-20% solvent thoroughly blended in; the PO additive produced a much "cheesier" final cured resin than equivalent percentage of acetone or EtOH. (However, see my Dec 6 2005 on-listserver message RE: LR White contrast, calling attention to SW Brorson's use of propylene oxide and/or high [accelerator] to improve exposure of antigens in epoxy sections to immunolabeling). We also explored the epoxy-anhydride ratio over a wide and quite heretical range (30-70% v/v anhydride) to finally settle on a "nicer" final cured resin (less brittle during block-trimming than the gospel ratio) because we found acceptable cutting, staining, and structural preservation over the whole range we tried.
Some issues I consider still unfinished: 1) Can shorter or colder (etc) staining of Epon(replacement)-DDSA or Spurr sections eliminate the nano-granularity to approach the finer-grained stain we routinely get in Araldite? 2) Does our Mn-Pb section stain perhaps "clog" or "fill in" some fine structures in the 5 nm and smaller range that are more delicately and clearly brought out by the Ur-Pb staining sequence? I've yet to re-check my impression that the 4.8 nm axial repeat on thick filaments routinely showed up 30 years ago on optical diffraction of insect muscle section EMs, before we started using the MnPb exclusively; and that it never shows up now that we no longer use the weaker contrasting UrPb sequence.
-mike reedy-
At 9:28 PM -0600 12/29/05, susan.vanhorn-at-stonybrook.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
The easiest way to get a transmitted light image on the 510 is to just click on ChD, which is a transmitted light detector. This will collect all the light that passes through your sample AT THE SAME TIME you are collecting confocal fluorescent images. So no need to double-expose your sample. You will still need to set the condenser and neutral density filters, etc., for Koehler.
I am not sure if it is a bug that the HAL light goes off when scanning - I think it is probably a protection (safety) so the PMTs do not get blasted.
-Holly __________________ Holly L. Aaron CRL Molecular Imaging Center http://imaging.berkeley.edu
-----Original Message----- X-from: lubo-at-berkeley.edu [mailto:lubo-at-berkeley.edu] Sent: Wednesday, December 28, 2005 6:46 AM To: hollya-at-berkeley.edu
Thanks guys, I really apreciate it. I will try some of the mentioned methods and I will update you with the results and I will tell you which method(s) worked with me.
Best Regards
Hany
On 12/30/05, ramadanhany-at-gmail.com {ramadanhany-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning, } } I grow tantalum oxide electrochemically on tantalum substrates, the } issue is that using SEM I can say that the surface of tantalum oxide } is full of defects "pin holes". I just use mechanical polishing of } tantalum before growing oxide, so I think that these defects result } from impurities of polishing sand papers. Is there any other way I can } polish tantalum to the finest grade avoiding these impurities? } } I appreciate your responses. } } Thanks } -- } ********************************************************** } Hany Ramadan } Graduate student } Chemistry department } McMaster university, Hamilton, Ontario, Canada } 905-525-9140 x: 26322 } elsayeh-at-mcmaster.ca } ********************************************************** } } } ==============================Original Headers============================== } 5, 23 -- From ramadanhany-at-gmail.com Fri Dec 30 10:10:44 2005 } 5, 23 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.201]) } 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id jBUGAiMs015284 } 5, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Dec 2005 10:10:44 -0600 } 5, 23 -- Received: by zproxy.gmail.com with SMTP id o37so2448119nzf } 5, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Dec 2005 08:10:44 -0800 (PST) } 5, 23 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 5, 23 -- s=beta; d=gmail.com; } 5, 23 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; } 5, 23 -- b=PYN5psgGfgI0WLNifAGGkP00f9GUSHXDkWrIi+bDVwtHrgVUiPHtTSNzH8/qCj4iWo1PnzfQMBQZCsCEzGDhrrwV0seSVvIrg57GrNfpfp4NrW/hMtWkQ+jXcNmfpDmh6jp5Cvjb4kwfB1DUnbR47y1HRX3kvEdsyqmEtn0yvqE= } 5, 23 -- Received: by 10.65.231.8 with SMTP id i8mr4207763qbr; } 5, 23 -- Fri, 30 Dec 2005 08:10:44 -0800 (PST) } 5, 23 -- Received: by 10.65.156.18 with HTTP; Fri, 30 Dec 2005 08:10:44 -0800 (PST) } 5, 23 -- Message-ID: {8d8ce5a30512300810o3ce7e534tc4e1ac19820b4cd4-at-mail.gmail.com} } 5, 23 -- Date: Fri, 30 Dec 2005 11:10:44 -0500 } 5, 23 -- From: Hany Ramadan {ramadanhany-at-gmail.com} } 5, 23 -- To: Microscopy-at-microscopy.com } 5, 23 -- Subject: Tantalum polishing } 5, 23 -- MIME-Version: 1.0 } 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1 } 5, 23 -- Content-Disposition: inline } 5, 23 -- Content-Transfer-Encoding: 8bit } 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id jBUGAiMs015284 } ==============================End of - Headers============================== }
-- ********************************************************** Hany Ramadan Graduate student Chemistry department McMaster university, Hamilton, Ontario, Canada 905-525-9140 x: 26322 elsayeh-at-mcmaster.ca **********************************************************
==============================Original Headers============================== 7, 25 -- From ramadanhany-at-gmail.com Tue Jan 3 18:28:12 2006 7, 25 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.193]) 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k040SCVw032542 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Jan 2006 18:28:12 -0600 7, 25 -- Received: by zproxy.gmail.com with SMTP id n29so4190029nzf 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 03 Jan 2006 16:28:12 -0800 (PST) 7, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 25 -- s=beta; d=gmail.com; 7, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 7, 25 -- b=jqvEITCtrHYGz22VW5v2TzDwTUPG6E7u8wxIz49Ct4T5ydsjuRU2DFVPLQemUrhx6KfdA71OlVgOpsJXvgrRMzHZ4Kd65rnXBquoj10M2tR4eawDB8YJdyC2xyIerEuZ1tzCcVsmxF6E6l/77PdQGmNx/sh55aJ32NRuNqyfplM= 7, 25 -- Received: by 10.65.177.14 with SMTP id e14mr4536714qbp; 7, 25 -- Tue, 03 Jan 2006 16:28:11 -0800 (PST) 7, 25 -- Received: by 10.65.156.18 with HTTP; Tue, 3 Jan 2006 16:28:11 -0800 (PST) 7, 25 -- Message-ID: {8d8ce5a30601031628o1cb70a8bre850d04cfd3b18d0-at-mail.gmail.com} 7, 25 -- Date: Tue, 3 Jan 2006 19:28:11 -0500 7, 25 -- From: Hany Ramadan {ramadanhany-at-gmail.com} 7, 25 -- To: Microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] Tantalum polishing 7, 25 -- In-Reply-To: {200512301648.jBUGmvLq022871-at-ns.microscopy.com} 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1 7, 25 -- Content-Disposition: inline 7, 25 -- References: {200512301648.jBUGmvLq022871-at-ns.microscopy.com} 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k040SCVw032542 ==============================End of - Headers==============================
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Title-Subject: [Filtered] SEMS annual meeting for 2006
Question: This year the annual meeting for the Southeastern Microscopy Society (SEMS) will be held jointly with the Association of Southeastern Biologists (ASB). The meeting will be March 29-April 1 in Gatlinburg, Tennessee at the Gatlinburg Convention Center. This event brings together approximately 800 biologists from across the southeastern United States. Interests are diverse, but range from genetics and molecular biology, to physiology and population biology, to community and ecosystem ecology.
Registration deadline is January 17, 2006 and information is available at the SEMS website:
http://www.semicroscopy.org/index.html or http://www.semicroscopy.org/meetings/call-for-papers.html
Cynthia S. Goldsmith Secretary, Southeastern Microscopy Society (SEMS) Mailstop G32 Centers for Disease Control and Prevention (CDC) Atlanta, GA 30333
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Title-Subject: [Filtered] Comparing cryo- and cryo-immuno diamond knives
Question: I would appreciate an advice from people who use cryo-immuno diamond knife from Diatome for the Tokuyasu technique. It is a newer model than their cryo diamond knife. Both can be seen at the following web site. http://www.emsdiasum.com/diatome/knife/immunocytochemistry.htm Is the newer model indeed better than the older one? Is there somebody in San Diego area who uses the cryo immuno knife? Thank you very much, Halina
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Email: rra-at-stowers-institute.org Name: Rhonda Allen
Organization: Stowers Institute
Title-Subject: [Filtered] glue to make ribbon's stick together
Question: Hello, I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. Thanks in adance. Rhonda Allen rra-at-stowers-institute.org
I seem to recall that diluted rubber cement can be used
rra-at-stowers-institute.org wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rra-at-stowers-institute.org as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 6, 12 -- From zaluzec-at-microscopy.com Tue Jan 3 20:11:11 2006 } 6, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 6, 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k042BADB010462 } 6, 12 -- for {microscopy-at-microscopy.com} ; Tue, 3 Jan 2006 20:11:10 -0600 } 6, 12 -- Mime-Version: 1.0 } 6, 12 -- X-Sender: (Unverified) } 6, 12 -- Message-Id: {p06110402bfe0df2a3482-at-[206.69.208.22]} } 6, 12 -- Date: Tue, 3 Jan 2006 20:11:08 -0600 } 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: rra-at-stowers-institute.org (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: glue to make ribbon's stick together } 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
-- Greg Erdos 5410 SE 185th Ave. Micanopy, FL 32667
==============================Original Headers============================== 3, 24 -- From gwe-at-ufl.edu Wed Jan 4 16:03:58 2006 3, 24 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 3, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k04M3v0Z007902 3, 24 -- for {microscopy-at-microscopy.com} ; Wed, 4 Jan 2006 16:03:58 -0600 3, 24 -- Received: from [192.168.1.97] (adsl-152-56-36.gnv.bellsouth.net [70.152.56.36]) 3, 24 -- (authenticated bits=0) 3, 24 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id k04M3tuh1663198; 3, 24 -- Wed, 4 Jan 2006 17:03:56 -0500 3, 24 -- Message-ID: {43BC4650.1010103-at-ufl.edu} 3, 24 -- Date: Wed, 04 Jan 2006 17:04:00 -0500 3, 24 -- From: greg erdos {gwe-at-ufl.edu} 3, 24 -- Reply-To: gwe-at-ufl.edu 3, 24 -- User-Agent: Thunderbird 1.5 (Windows/20051201) 3, 24 -- MIME-Version: 1.0 3, 24 -- To: rra-at-stowers-institute.org, microscopy-at-microscopy.com 3, 24 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 3, 24 -- References: {200601040223.k042NquA014469-at-ns.microscopy.com} 3, 24 -- In-Reply-To: {200601040223.k042NquA014469-at-ns.microscopy.com} 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 3, 24 -- X-UFL-Spam-Status: hits=-1.524, required=5, tests=BAYES_01 3, 24 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 24 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tsoumt-at-ilan.tpg.gov.tw as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tsoumt-at-ilan.tpg.gov.tw Name: Jack Tsou
Organization: I-lan Hospital, Taiwan
Title-Subject: [Filtered] Has anyone successfully mounted Canon pro1 onto trinocular port of Olympus BX51?
Question: I'm quite interested in the digital camera Canon pro1 and would like to know the detail if anybody had ever successfully mounted it onto the trinocular port of BX51. Thanks in advance!
If you haven't committed to the Canon Powershot Pro1 yet, I strongly recommend you move up to a camera with a removable lens like the Canon EOS 350D or The Nikon D50 - and use the Microscope as the lens.
On 5 Jan 2006, at 19:57, tsoumt-at-ilan.tpg.gov.tw wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying please copy both tsoumt-at-ilan.tpg.gov.tw as well as } the MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: tsoumt-at-ilan.tpg.gov.tw } Name: Jack Tsou } } Organization: I-lan Hospital, Taiwan } } Title-Subject: [Filtered] Has anyone successfully mounted Canon pro1 } onto trinocular port of Olympus BX51? } } Question: I'm quite interested in the digital camera Canon pro1 and } would like to know the detail if anybody had ever successfully mounted } it onto the trinocular port of BX51. Thanks in advance! } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 6, 12 -- From } zaluzec-at-microscopy.com Thu Jan 5 17:20:05 2006 6, 12 -- Received: } from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 12 -- by } ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k05NJrdL005222 6, 12 } -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2006 17:19:53 -0600 6, } 12 -- Mime-Version: 1.0 6, 12 -- X-Sender: (Unverified) 6, 12 -- } Message-Id: {p06110400bfe35a0001a2-at-[206.69.208.22]} 6, 12 -- Date: } Thu, 5 Jan 2006 17:19:47 -0600 6, 12 -- To: microscopy-at-microscopy.com } 6, 12 -- From: tsoumt-at-ilan.tpg.gov.tw (by way of MicroscopyListserver) } 6, 12 -- Subject: viaWWW: Canon pro1 onto trinocular port of Olympus } BX51 6, 12 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 6 08:33:25 2006 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06EXIIR006521 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 08:33:19 -0600 10, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k06EWWHB001896; 10, 25 -- Fri, 6 Jan 2006 09:32:32 -0500 10, 25 -- Received: from emf03 ([134.53.14.97]) 10, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k06EWWSt016030; 10, 25 -- Fri, 6 Jan 2006 09:32:32 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: tsoumt-at-ilan.tpg.gov.tw 10, 25 -- Date: Fri, 6 Jan 2006 09:33:29 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Subject: Re: [Microscopy] viaWWW: Canon pro1 onto trinocular port of Olympus BX51 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {43BE3969.5908.ECA3945-at-localhost} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200601060157.k061vebc022853-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 10, 25 -- X-Real-ConnectIP: 134.53.14.97 10, 25 -- X-Scanned-By: MIMEDefang 2.45 10, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 ==============================End of - Headers==============================
Hello everybody, One of our EM lab users wants to buy triangular tungsten carbide knives for serial sectioning. Is there any advantage in buying that kind of knife? What is a life span (how many blocks/sections can be cut before it becomes dull)? Can they be resharpen? Are they good for EM resins (Epon, Spurrs ect.)? Thanks Dorota
==============================Original Headers============================== 1, 22 -- From wadowska-at-avcn1.novell.upei.ca Fri Jan 6 11:00:05 2006 1, 22 -- Received: from mx.upei.ca (humboldt.cs.upei.ca [137.149.3.10]) 1, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H014x010343 1, 22 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 11:00:02 -0600 1, 22 -- Received: from [137.149.64.250] (helo=acad1.cs.upei.ca) 1, 22 -- by mx.upei.ca with esmtp (Exim 3.35 #1 (Debian)) 1, 22 -- id 1EuuwX-00070Q-01 1, 22 -- for {microscopy-at-microscopy.com} ; Fri, 06 Jan 2006 12:59:57 -0400 1, 22 -- Received: from AVCN1/SpoolDir by acad1.cs.upei.ca (Mercury 1.48); 1, 22 -- 6 Jan 06 12:59:57 -0400 1, 22 -- Received: from SpoolDir by AVCN1 (Mercury 1.48); 6 Jan 06 12:59:36 -0400 1, 22 -- From: "Dorota Wadowska" {wadowska-at-upei.ca} 1, 22 -- Organization: University of P.E.I. 1, 22 -- To: microscopy-at-microscopy.com 1, 22 -- Date: Fri, 6 Jan 2006 12:10:46 -0400 1, 22 -- MIME-Version: 1.0 1, 22 -- Content-type: text/plain; charset=US-ASCII 1, 22 -- Content-transfer-encoding: 7BIT 1, 22 -- Subject: LM tungsten carbide knives 1, 22 -- Message-ID: {43BE5E46.13266.E4BB6B-at-localhost} 1, 22 -- Priority: normal 1, 22 -- X-mailer: Pegasus Mail for Win32 (v3.12c) ==============================End of - Headers==============================
For glue to get section ribbons to stick together, I like to use Weldwood Contact Cement (avialable at your local hardware store) diluted 1:1 in xylene. I picked up this tip somewhere a few years ago, but don't remember where. Maybe it was from Microscopy!
I assume you have trimed the block to be a 4 sided pyramid with a flat top being the blockface from which sections will be cut. To apply glue, use a small end of a toothpick, dip in diluted glue, and dab onto one of the 4 sides of the block face, either top or bottom relative to orientation of block during sectioning. I usually tilt the block face so that the side I choose is nearly horizontal so the glue doesn't run down the block side, or over the actual face of the block. Be careful so that glue does not get onto the blockface itself. Also, work quickly as the glue will dry fast. Then reorient the block for sectioning.
There is also a product out there called Tacky Wax, available from EM supply companies, which you dab onto the side of the block face. You may or may not need to apply gentle heat near it to get it to spread evenly over the side of the blockface. Perhaps others may wish to comment on the use of Tacky Wax.
Hope this helps you!
Gib ---- Gib Ahlstrand Imaging Center University of Minbnesota St. Paul, MN 55108 ahlst007-at-umn.eud http://www.cbs.umn.edu/ic
rra-at-stowers-institute.org wrote: } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org
==============================Original Headers============================== 7, 20 -- From ahlst007-at-umn.edu Fri Jan 6 11:02:56 2006 7, 20 -- Received: from mtaout-m.tc.umn.edu (mtaout-m.tc.umn.edu [160.94.23.21]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H2oln010411 7, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 11:02:55 -0600 7, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-m.tc.umn.edu with ESMTP; Fri, 6 Jan 2006 11:02:44 -0600 (CST) 7, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 7, 20 -- Message-ID: {43BEA2AF.7030808-at-umn.edu} 7, 20 -- Date: Fri, 06 Jan 2006 11:02:39 -0600 7, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 7, 20 -- Reply-To: ahlst007-at-umn.edu 7, 20 -- Organization: Imaging Center UM 7, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: rra-at-stowers-institute.org, Microscopy-at-Microscopy.com 7, 20 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 7, 20 -- References: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Job Opening #B005608; Lab Technician/Engineer at IBM
There is an opening at the Lab Technician/Engineer level within the Materials Characterization and Analysis Group in the Research Division of IBM at the Almaden Research Center in San Jose, California. The job is a long term (three years) supplemental position with benefits.This position involves technical support in an advanced electron microscopy laboratory which emphasizes transmission electron microscopy. The technician works as a team member with professional (Ph.D.) microscopists and other scientists in a research laboratory. Duties involve primarily sample preparation methods including the conventional grinding, polishing, and ion milling techniques, focused ion beam (FIB ) method, as well as Microtome, etc. In addition, the candidate must be able to operate and perform routine maintenance on specimen preparation equipment, interact with customers (professional scientists), and prepare reports. The candidate must be able to work independently within set priorities, to keep abreast of new advances, and to interact smoothly within a team.
Experience with sample preparation and basic TEM imaging is a plus.
IBM is an equal opportunity employer. Women and Minorities are encouraged to apply. Information on the Almaden Research Center can be found at www.almaden.ibm.com
Interested parties may call or send resumes to me at the following address:
Dr. Philip M. Rice IBM Research Division Almaden Research Center 650 Harry Road, K19B/D1 tel: (408) 927-1442 fax: (408) 927-2100 email: pmrice-at-almaden.ibm.com
==============================Original Headers============================== 10, 26 -- From pmrice-at-almaden.ibm.com Fri Jan 6 11:23:44 2006 10, 26 -- Received: from e2.ny.us.ibm.com (e2.ny.us.ibm.com [32.97.182.142]) 10, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06HMhYt011005 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 11:23:10 -0600 10, 26 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 10, 26 -- by e2.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id k06HMdHj029131 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:39 -0500 10, 26 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 10, 26 -- by d01relay02.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id k06HMd87122936 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:39 -0500 10, 26 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 10, 26 -- by d01av01.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k06HMdBn004984 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:39 -0500 10, 26 -- Received: from d01ml605.pok.ibm.com (d01ml605.pok.ibm.com [9.56.227.91]) 10, 26 -- by d01av01.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k06HMck9004981 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 6 Jan 2006 12:22:38 -0500 10, 26 -- Subject: TEM - Job Opening for TEM Specimen Preparation Lab Technician 10, 26 -- To: microscopy-at-microscopy.com 10, 26 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 10, 26 -- Message-ID: {OFBC79C14C.36D1DACC-ON882570EE.005F03ED-882570EE.005F72CD-at-us.ibm.com} 10, 26 -- From: Philip Rice {pmrice-at-almaden.ibm.com} 10, 26 -- Date: Fri, 6 Jan 2006 09:22:33 -0800 10, 26 -- X-MIMETrack: Serialize by Router on D01ML605/01/M/IBM(Release 7.0HF90 | November 16, 2005) at 10, 26 -- 01/06/2006 12:22:38 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gsosinsky-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gsosinsky-at-ucsd.edu Name: Gina Sosinsky
Organization: University of California, San Diego
Title-Subject: [Filtered] 4th International Congress for Electron Tomography 2006
Question: **************** First Announcement *************** The Fourth International Congress on Electron Tomography, (4ICET), will be held at Paradise Point, San Diego November 5-8, 2006.
This congress brings together biologists, biophysicists, computer scientists, mathematicians, materials scientists and electron optical instrumentation specialists for an interdisciplinary exchange of ideas focusing on advancing methods of electron tomography (ET) in biology. ET has moved from a specialized experimental technique practiced by a few laboratories to one delivering critical information to a broad community of cell biologists, structural biologists, and neuroscientists. For students, this conference presents a unique opportunity to learn about cutting-edge advances in ET applications and methodologies.
Sessions will include:
ļ Imaging of dynamic structures and correlative microscopy Co-Chairs: O. Medalia (Ben-Gurion Univ.); Jack Johnson (The Scripps Research Institute)
ļ Advances in instrumentation Co-chairs: M. Ellisman (UCSD); Bram Koster (Univ. ofUltrect, Netherlands)
ļ 3D reconstruction algorithms Co-chairs: N. Volkmann (Burnham Institute); TBA
ļ Visualization & quantitative analysis Co-chairs: R. Whitaker (Univ. of Utah); D. Mastronarde (Univ. of Colorado)
ļ Moving tomography to the mainstream: data sharing, data integration, & model building Co-chairs: M. Martone (UCSD); J-M. Carazo (Univ. of Madrid)
ļ Emerging technologies for the multiscale: cell to tissue and molecule to cell Co-chairs: D. Hanein (Burnham Institute); C. Larabell (UCSF)
Sessions will include both invited speakers and talks selected from submitted abstracts.
For further information please check out our web site: http://www.4icet.org
or contact Mark H. Ellisman, Lead Congress Organizer (mark-at-ncmir.ucsd.edu) or Grace Osborne, Congress Coordinator (gosborne-at-ncmir,ucsd.edu)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gsosinsky-at-ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gsosinsky-at-ucsd.edu Name: Gina Sosinsky
Organization: University of California, San Diego
Title-Subject: [Filtered] 4th International Congress for Electron Tomography 2006
Question: **************** First Announcement *************** The Fourth International Congress on Electron Tomography, (4ICET), will be held at Paradise Point, San Diego November 5-8, 2006.
This congress brings together biologists, biophysicists, computer scientists, mathematicians, materials scientists and electron optical instrumentation specialists for an interdisciplinary exchange of ideas focusing on advancing methods of electron tomography (ET) in biology. ET has moved from a specialized experimental technique practiced by a few laboratories to one delivering critical information to a broad community of cell biologists, structural biologists, and neuroscientists. For students, this conference presents a unique opportunity to learn about cutting-edge advances in ET applications and methodologies.
Sessions will include:
ļ Imaging of dynamic structures and correlative microscopy Co-Chairs: O. Medalia (Ben-Gurion Univ.); Jack Johnson (The Scripps Research Institute)
ļ Advances in instrumentation Co-chairs: M. Ellisman (UCSD); Bram Koster (Univ. ofUltrect, Netherlands)
ļ 3D reconstruction algorithms Co-chairs: N. Volkmann (Burnham Institute); TBA
ļ Visualization & quantitative analysis Co-chairs: R. Whitaker (Univ. of Utah); D. Mastronarde (Univ. of Colorado)
ļ Moving tomography to the mainstream: data sharing, data integration, & model building Co-chairs: M. Martone (UCSD); J-M. Carazo (Univ. of Madrid)
ļ Emerging technologies for the multiscale: cell to tissue and molecule to cell Co-chairs: D. Hanein (Burnham Institute); C. Larabell (UCSF)
Sessions will include both invited speakers and talks selected from submitted abstracts.
For further information please check out our web site: http://www.4icet.org
or contact Mark H. Ellisman, Lead Congress Organizer (mark-at-ncmir.ucsd.edu) or Grace Osborne, Congress Coordinator (gosborne-at-ncmir,ucsd.edu)
Year 10 was last year: I hope you can join us as the UBC Course enters its second decade.
Speaking for myself, and I am sure for our faculty, it is a source of great satisfaction to see how many of the contributors to this list are UBC Course Alumnae.
Thank you all.
Jim P.
Eleventh Annual INTERNATIONAL 12-Day Short Course on 3D Microscopy of Living Cells, June 10 - 22, 2006 (Pre-course: June 10)
Tenth, Post-course Workshop on 3D Image Processing, June 24 -26, 2006
Organized by Prof. James Pawley, (University of Wisconsin-Madison)
in association with the Departments of Pharmacology and Physiology and the Brain Research Centre, University of British Columbia, Vancouver, BC, Canada
DATES
Applications must be received by Tuesday, March 15, 2006 Deposit due Friday, April 15, 2006 Registration 5:00 - 7:00 PM Saturday, June 10, 2006 First Lecture 7:30 PM Saturday, June 10, 2006 Live-cell Course ends, noon Thursday, June 22, 2006 3D Image Processing Course, Saturday, June 24 - Monday, 26, 2006
APPLICATIONS DUE BY MARCH 15, 2006
APPLICATIONS Applicants must complete a questionnaire on the web to assess knowledge level, field of interest and proposed personal, live-cell, project. But don't let this put you off: if you plan to use 3D microscopy on living cells, we can usually find a way to make it work.
Enrollment will be limited to about 32 participants (exact number depends on number of 3D Systems available). Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with access to basic texts to read before the course begins. Application forms may be down-loaded from the WWW site at
http://www.3dcourse.ubc.ca/application.htm , or obtained from:
Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-265-5315 250 N. Mills Street, Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
Additional information is available from: http://www.3dcourse.ubc.ca/brochure.htm and links.
We expect to have at least 11, 3D microscope workstations for student use and there will be an international faculty of 20.
Application deadlines:
Application forms should be received for screening by March 15, 2006. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2006. In general, refunds of the deposit will only be possible if someone on the waiting list can take the place but this has not been a problem in previous years. The remaining balance is due before Registration.
Pre-Course Tuition (1/2 day Basic Optical principles) $125 (US) 3D Live-cell Course Tuition (includes lunches, snacks, 3 dinners, incl. the NEW Third Edition of the Handbook of Biological Confocal Microscopy): $2,750 (US) Workshop Tuition (includes lunches and snacks): $1,100 (US)
Room/board about $40/day (US) depending on room type.
I hope that this includes all of the information that you need, but if not, please get back to me.
Jim Pawley
-- **************************************** Prof. James B. Pawley, Ph. 608-263-3147 Room 223, Zoology Research Building, FAX 608-262-9083 250 N. Mills St., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU "A scientist is not one who can answer questions but one who can question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39
==============================Original Headers============================== 27, 24 -- From jbpawley-at-wisc.edu Fri Jan 6 12:54:20 2006 27, 24 -- Received: from smtp4.wiscmail.wisc.edu (hermes.doit.wisc.edu [144.92.197.190]) 27, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06IrMoO013779 27, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 6 Jan 2006 12:53:44 -0600 27, 24 -- Received: from avs-daemon.smtp4.wiscmail.wisc.edu by smtp4.wiscmail.wisc.edu 27, 24 -- (iPlanet Messaging Server 5.2 HotFix 2.08 (built Sep 22 2005)) 27, 24 -- id {0ISO0090SPSXW6-at-smtp4.wiscmail.wisc.edu} for Microscopy-at-Microscopy.Com; 27, 24 -- Fri, 06 Jan 2006 12:53:21 -0600 (CST) 27, 24 -- Received: from [144.92.238.207] by smtp4.wiscmail.wisc.edu 27, 24 -- (iPlanet Messaging Server 5.2 HotFix 2.08 (built Sep 22 2005)) 27, 24 -- with ESMTPSA id {0ISO00LB4PSRMA-at-smtp4.wiscmail.wisc.edu} for 27, 24 -- Microscopy-at-Microscopy.Com; Fri, 06 Jan 2006 12:53:16 -0600 (CST) 27, 24 -- Date: Fri, 06 Jan 2006 12:53:14 -0600 27, 24 -- From: James Pawley {jbpawley-at-wisc.edu} 27, 24 -- Subject: First announcement: Eleventh International UBC Live-cell Course. 27, 24 -- X-Sender: jbpawley-at-wiscmail.wisc.edu 27, 24 -- To: "ListServer-at-MSA.Microscopy.Com" {Microscopy-at-Microscopy.Com} 27, 24 -- Message-id: {p06110415bfe46d18adc1-at-[144.92.238.207]} 27, 24 -- MIME-version: 1.0 27, 24 -- Content-type: text/plain; format=flowed; charset=us-ascii 27, 24 -- Content-transfer-encoding: 7BIT 27, 24 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=0.0.0.0 27, 24 -- X-Spam-PmxInfo: Server=avs-8, Version=5.1.1.222179, Antispam-Engine: 2.1.0.0, 27, 24 -- Antispam-Data: 2006.1.6.19, SenderIP=0.0.0.0 ==============================End of - Headers==============================
In our lab, there was a person using a thin coat of dental wax successfully on bottom side of a block. Melt dental in a beaker and apply a tiny bit to a shaped block with a warm spatula. You may have to trim out excess wax.
I have Tackiwax also. It came in a tiny bottle. It seems to me that it is repackaged dental wax without color. On the other hand, I may be wrong this material.
Ann Fook Yang EM Unit/ Unite EM AAFC/AAC 960 Carling Ave, Ottawa,Ontario Canada K1A 0C6 yanga-at-agr.gc.ca Telephone/Téléphone: 613-759-1638 Facsimile/Télécopieur: 613-759-1701
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: ahlst007-at-umn.edu [mailto:ahlst007-at-umn.edu] Sent: Friday, January 06, 2006 5:35 PM To: Yang, Ann-Fook
Rhonda,
For glue to get section ribbons to stick together, I like to use Weldwood Contact Cement (avialable at your local hardware store) diluted 1:1 in xylene. I picked up this tip somewhere a few years ago, but don't remember where. Maybe it was from Microscopy!
I assume you have trimed the block to be a 4 sided pyramid with a flat top being the blockface from which sections will be cut. To apply glue, use a small end of a toothpick, dip in diluted glue, and dab onto one of the 4 sides of the block face, either top or bottom relative to orientation of block during sectioning. I usually tilt the block face so that the side I choose is nearly horizontal so the glue doesn't run down the block side, or over the actual face of the block. Be careful so that glue does not get onto the blockface itself. Also, work quickly as the glue will dry fast. Then reorient the block for sectioning.
There is also a product out there called Tacky Wax, available from EM supply companies, which you dab onto the side of the block face. You may or may not need to apply gentle heat near it to get it to spread evenly over the side of the blockface. Perhaps others may wish to comment on the use of Tacky Wax.
Hope this helps you!
Gib ---- Gib Ahlstrand Imaging Center University of Minbnesota St. Paul, MN 55108 ahlst007-at-umn.eud http://www.cbs.umn.edu/ic
rra-at-stowers-institute.org wrote: } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org
==============================Original Headers============================== 7, 20 -- From ahlst007-at-umn.edu Fri Jan 6 11:02:56 2006 7, 20 -- Received: from mtaout-m.tc.umn.edu (mtaout-m.tc.umn.edu [160.94.23.21]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H2oln010411 7, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 11:02:55 -0600 7, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-m.tc.umn.edu with ESMTP; Fri, 6 Jan 2006 11:02:44 -0600 (CST) 7, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 7, 20 -- Message-ID: {43BEA2AF.7030808-at-umn.edu} 7, 20 -- Date: Fri, 06 Jan 2006 11:02:39 -0600 7, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 7, 20 -- Reply-To: ahlst007-at-umn.edu 7, 20 -- Organization: Imaging Center UM 7, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: rra-at-stowers-institute.org, Microscopy-at-Microscopy.com 7, 20 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 7, 20 -- References: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From YANGA-at-AGR.GC.CA Mon Jan 9 09:25:19 2006 16, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 16, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k09FPHkv029897 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 09:25:18 -0600 16, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 16, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FPDHp017974 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:25:13 -0500 16, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 16, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FQiUY023252 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:26:45 -0500 16, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 16, 29 -- Mon, 9 Jan 2006 10:25:08 -0500 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 16, 29 -- content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="iso-8859-1" 16, 29 -- Subject: RE: [Microscopy] glue to make ribbon's stick together 16, 29 -- Date: Mon, 9 Jan 2006 10:25:06 -0500 16, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB10243345A-at-onncrxms3.agr.gc.ca} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] glue to make ribbon's stick together 16, 29 -- Thread-Index: AcYT2iOC0AiPsPOzQuGBu5lpBYTxtgBUcMiw 16, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 16, 29 -- To: {microscopy-at-microscopy.com} 16, 29 -- X-OriginalArrivalTime: 09 Jan 2006 15:25:08.0115 (UTC) FILETIME=[DFA16E30:01C61530] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k09FPHkv029897 ==============================End of - Headers==============================
Does anyone know if the various glues suggested vaporise in the TEM? If so does this cause any problems?
Dave
-----Original Message----- X-from: YANGA-at-AGR.GC.CA [mailto:YANGA-at-AGR.GC.CA] Sent: 09 January 2006 15:28 To: David Patton
In our lab, there was a person using a thin coat of dental wax successfully on bottom side of a block. Melt dental in a beaker and apply a tiny bit to a shaped block with a warm spatula. You may have to trim out excess wax.
I have Tackiwax also. It came in a tiny bottle. It seems to me that it is repackaged dental wax without color. On the other hand, I may be wrong this material.
Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire Canada
-----Original Message----- X-from: ahlst007-at-umn.edu [mailto:ahlst007-at-umn.edu] Sent: Friday, January 06, 2006 5:35 PM To: Yang, Ann-Fook
Rhonda,
For glue to get section ribbons to stick together, I like to use Weldwood Contact Cement (avialable at your local hardware store) diluted 1:1 in xylene. I picked up this tip somewhere a few years ago, but don't remember where. Maybe it was from Microscopy!
I assume you have trimed the block to be a 4 sided pyramid with a flat top being the blockface from which sections will be cut. To apply glue, use a small end of a toothpick, dip in diluted glue, and dab onto one of the 4 sides of the block face, either top or bottom relative to orientation of block during sectioning. I usually tilt the block face so that the side I choose is nearly horizontal so the glue doesn't run down the block side, or over the actual face of the block. Be careful so that glue does not get onto the blockface itself. Also, work quickly as the glue will dry fast. Then reorient the block for sectioning.
There is also a product out there called Tacky Wax, available from EM supply companies, which you dab onto the side of the block face. You may or may not need to apply gentle heat near it to get it to spread evenly over the side of the blockface. Perhaps others may wish to comment on the use of Tacky Wax.
Hope this helps you!
Gib ---- Gib Ahlstrand Imaging Center University of Minbnesota St. Paul, MN 55108 ahlst007-at-umn.eud http://www.cbs.umn.edu/ic
rra-at-stowers-institute.org wrote: } Email: rra-at-stowers-institute.org } Name: Rhonda Allen } } Organization: Stowers Institute } } Title-Subject: [Filtered] glue to make ribbon's stick together } } Question: Hello, } I was wondering if any of you could tell me what kind of glue is used to make spurr's ribbon's stick together. I need to do serial sections and collect them all. Since I am new to this I am open for any and all suggestions. } Thanks in adance. } Rhonda Allen } rra-at-stowers-institute.org
==============================Original Headers============================== 7, 20 -- From ahlst007-at-umn.edu Fri Jan 6 11:02:56 2006 7, 20 -- Received: from mtaout-m.tc.umn.edu (mtaout-m.tc.umn.edu [160.94.23.21]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k06H2oln010411 7, 20 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 Jan 2006 11:02:55 -0600 7, 20 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) by mtaout-m.tc.umn.edu with ESMTP; Fri, 6 Jan 2006 11:02:44 -0600 (CST) 7, 20 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 7, 20 -- Message-ID: {43BEA2AF.7030808-at-umn.edu} 7, 20 -- Date: Fri, 06 Jan 2006 11:02:39 -0600 7, 20 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 7, 20 -- Reply-To: ahlst007-at-umn.edu 7, 20 -- Organization: Imaging Center UM 7, 20 -- User-Agent: Mozilla Thunderbird 1.0.7 (Macintosh/20050923) 7, 20 -- X-Accept-Language: en-us, en 7, 20 -- MIME-Version: 1.0 7, 20 -- To: rra-at-stowers-institute.org, Microscopy-at-Microscopy.com 7, 20 -- Subject: Re: [Microscopy] viaWWW: glue to make ribbon's stick together 7, 20 -- References: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- In-Reply-To: {200601040322.k043ML3t002256-at-ns.microscopy.com} 7, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From YANGA-at-AGR.GC.CA Mon Jan 9 09:25:19 2006 16, 29 -- Received: from agroutsmtp1.agr.gc.ca (agroutsmtp1.agr.gc.ca [192.197.71.175]) 16, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k09FPHkv029897 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 09:25:18 -0600 16, 29 -- Received: from agrin1-old.agr.gc.ca (agrgate.agr.ca [192.197.71.189]) 16, 29 -- by agroutsmtp1.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FPDHp017974 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:25:13 -0500 16, 29 -- Received: from onncrxcn1.AGR.GC.CA ([10.117.15.130]) 16, 29 -- by agrin1-old.agr.gc.ca (8.12.8/8.12.8) with ESMTP id k09FQiUY023252 16, 29 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:26:45 -0500 16, 29 -- Received: from onncrxms3.AGR.GC.CA ([10.117.15.30]) by onncrxcn1.AGR.GC.CA with Microsoft SMTPSVC(5.0.2195.6713); 16, 29 -- Mon, 9 Jan 2006 10:25:08 -0500 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 16, 29 -- content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="iso-8859-1" 16, 29 -- Subject: RE: [Microscopy] glue to make ribbon's stick together 16, 29 -- Date: Mon, 9 Jan 2006 10:25:06 -0500 16, 29 -- Message-ID: {E035A9C87303AE4AB9BA10FD8324DFB10243345A-at-onncrxms3.agr.gc.ca} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] glue to make ribbon's stick together 16, 29 -- Thread-Index: AcYT2iOC0AiPsPOzQuGBu5lpBYTxtgBUcMiw 16, 29 -- From: "Yang, Ann-Fook" {YANGA-at-AGR.GC.CA} 16, 29 -- To: {microscopy-at-microscopy.com} 16, 29 -- X-OriginalArrivalTime: 09 Jan 2006 15:25:08.0115 (UTC) FILETIME=[DFA16E30:01C61530] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k09FPHkv029897 ==============================End of - Headers==============================
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==============================Original Headers============================== 29, 33 -- From David.Patton-at-uwe.ac.uk Mon Jan 9 09:32:45 2006 29, 33 -- Received: from mailapp02.uwe.ac.uk (mailapp02.uwe.ac.uk [164.11.132.63]) 29, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k09FWhRa002940 29, 33 -- for {Microscopy-at-Microscopy.com} ; Mon, 9 Jan 2006 09:32:44 -0600 29, 33 -- Received: from (164.11.132.62) by mailapp02.uwe.ac.uk via smtp 29, 33 -- id 0461_8549066c_8125_11da_8b14_0002b3c90020; 29, 33 -- Mon, 09 Jan 2006 15:35:11 +0000 29, 33 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 29, 33 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 29, 33 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 29, 33 -- 2005)) with ESMTP id {0ISU00H0T0IHDB-at-mta02.uwe.ac.uk} for 29, 33 -- Microscopy-at-Microscopy.com; Mon, 09 Jan 2006 15:32:41 +0000 (GMT) 29, 33 -- Date: Mon, 09 Jan 2006 15:32:40 +0000 29, 33 -- From: David Patton {David.Patton-at-uwe.ac.uk} 29, 33 -- Subject: RE: [Microscopy] RE: glue to make ribbon's stick together 29, 33 -- To: Microscopy-at-Microscopy.com 29, 33 -- Message-id: {F247F674896BE243AD8263C5280E2BDBF8507D-at-egen-uwe01} 29, 33 -- MIME-version: 1.0 29, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 29, 33 -- Content-type: text/plain; 29, 33 -- charset="utf-8" 29, 33 -- Content-class: urn:content-classes:message 29, 33 -- Thread-topic: [Microscopy] RE: glue to make ribbon's stick together 29, 33 -- Thread-index: AcYVMVNhlhbSoF3kQxeNI9avIx8RPAAAF3QQ 29, 33 -- X-MS-Has-Attach: 29, 33 -- X-MS-TNEF-Correlator: 29, 33 -- X-NAI-Spam-Score: -0.3 29, 33 -- X-NAI-Spam-Rules: 1 Rules triggered 29, 33 -- BAYES_30=-0.3 29, 33 -- X-NAIMIME-Disclaimer: 1 29, 33 -- X-NAIMIME-Modified: 1 29, 33 -- Content-Transfer-Encoding: 8bit 29, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k09FWhRa002940 ==============================End of - Headers==============================
I posted this question a couple of weeks ago, but probably the timing was wrong (just before the holidays), no answers at all. So I am posting again:
I would need a tip on a good (commercially available) antibody against His tag for EM - Tokuyasu technique. It should tolerate at least light (0.1-0.2%) glutaraldehyde fixation. A rabbit polyclonal would be preferable, but a working monoclonal would do as well.
Another unrelated question - I would need to label macrophages in sections (again, Tokuyasu). Any idea for a good macrophage marker?
Thanks,
Michal
==============================Original Headers============================== 7, 19 -- From M_Jarnik-at-fccc.edu Mon Jan 9 10:03:32 2006 7, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 7, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k09G3VhJ016139 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 10:03:32 -0600 7, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 7, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k09G3USd021508 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 9 Jan 2006 11:03:30 -0500 (EST) 7, 19 -- Message-ID: {43C28952.4040809-at-fccc.edu} 7, 19 -- Date: Mon, 09 Jan 2006 11:03:30 -0500 7, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 7, 19 -- Reply-To: M_Jarnik-at-fccc.edu 7, 19 -- Organization: Fox Chase Cancer Center 7, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 7, 19 -- X-Accept-Language: en,cs 7, 19 -- MIME-Version: 1.0 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- Subject: His Tag antibody for EM 7, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 7, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjo10-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mjo10-at-psu.edu Name: Matthew Olszta
Organization: Penn State University
Title-Subject: [Filtered] Vanadium oxide thin films
Question: I wanted to know if anyone has had any experience with TEM sample preparation and analysis of vanadium oxide thin films. The reason I ask is that certain vanadium oxide crystal structures (VO2 and V2O5) are soluble or slightly soluble in water, but using water in polishing I have not yet seen any appreciable dissolution in my cross-sections.
If others have worked with these materials, or other water sensitive materials, do you have any suggestions or comments on sample preparation in order to avoid artifacts?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjo10-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mjo10-at-psu.edu Name: Matthew Olszta
Organization: Penn State University
Title-Subject: [Filtered] Vanadium oxide thin films
Question: I wanted to know if anyone has had any experience with TEM sample preparation and analysis of vanadium oxide thin films. The reason I ask is that certain vanadium oxide crystal structures (VO2 and V2O5) are soluble or slightly soluble in water, but using water in polishing I have not yet seen any appreciable dissolution in my cross-sections.
If others have worked with these materials, or other water sensitive materials, do you have any suggestions or comments on sample preparation in order to avoid artifacts?
I am not sure about vaporization issues, but I have used another glue. Using a sharpened toothpick dipped in acetone, I have dissolved glue from 3M adhesive tape ("Magic Tape") to apply thin amounts of glue to the top surface of the block when trying to obtain serial sections. I have not noted any contamination problems, but clearly you want to refrain from getting any of this solvent on the cutting face.
However, when collecting serial sections, glue should only be your last resort. When properly trimmed with a fresh razor blade (I like the chromium steel blades from "Wilkinson Sword Blades") so that both top edge and bottom edge are parallel to each other and to the diamond knife edge, most Epon-like resins allow serial sections to stick together quite well. I don't know how well this applies to Spurr's in particular, but I expect the same results. The cleaner those edges are, and the more parallel they are to each other and to the diamond, the better the result. Acrylic resins like LR White or LR Gold tend to be much more brittle, and those would be more difficult to handle in series.
Dave Hall
For a more exhaustive discussion of serial sectioning, see Hall (1995) Methods in Cell Biology, C. elegans: Modern Biological Analysis of an Organism. Epstein and Shakes (eds) Academic Press. pp. 395-436. -- David H. Hall, Ph.D. Center for C. elegans Anatomy Department of Neuroscience Albert Einstein College of Medicine 1410 Pelham Parkway Bronx, NY 10461
www.wormatlas.org www.aecom.yu.edu/wormem
phone 718 430-2195 fax 718 430-2514
==============================Original Headers============================== 6, 21 -- From hall-at-aecom.yu.edu Tue Jan 10 10:06:01 2006 6, 21 -- Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0AG61X7031584 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 10:06:01 -0600 6, 21 -- Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) 6, 21 -- by mailgw.aecom.yu.edu (8.12.11/8.12.11) with SMTP id k0AG60vS020160 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 11:06:00 -0500 6, 21 -- Received: from post.aecom.yu.edu ([129.98.1.100]) 6, 21 -- by mailvx.aecom.yu.edu (SAVSMTP 3.1.1.32) with SMTP id M2006011011062608439 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 11:06:26 -0500 6, 21 -- Received: from [129.98.90.160] (worm.aecom.yu.edu [129.98.90.160]) 6, 21 -- by post.aecom.yu.edu (Postfix) with ESMTP id 340A12FC0 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 11:06:00 -0500 (EST) 6, 21 -- Mime-Version: 1.0 6, 21 -- X-Sender: hall-at-mailserver.aecom.yu.edu 6, 21 -- Message-Id: {a05100316bfe98b41a356-at-[129.98.90.160]} 6, 21 -- Date: Tue, 10 Jan 2006 11:05:56 -0500 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- From: David Hall {hall-at-aecom.yu.edu} 6, 21 -- Subject: glue to make ribbon's stick together 6, 21 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Matt, I don't have experience to work with vanadium oxide, but some superconductive materials which were not working well with water. If you use water only for your grinding procedure for your case, now you can try dry-grinding, at least for the final stage. or you can try leaving thicker for the final ion mill (like 45 micron) if it is not hard to be ion-milled under Ar+ ion beam, but this may cause some beam damage or contamination if there is not cold stage and the milling time is too long. again to try higher rate for the early milling stage and to reduce it at the final may help with this. or you could try methanol or ethanol for your grinding if the "wet" procedure is necessary. also you can try FIB. it is dry, but may consider its beam damage if your material is sensitive to this. Hope it can help
Sincerely Long Li _________________________________________________________ Materials Science and Engineering Department University of Pittsburgh 3700 O'Hara St., 848 Benedum Hall Pittsburgh, PA 15261
----- Original Message ----- X-from: {mjo10-at-psu.edu} To: {Longli_tem-at-hotmail.com} Sent: Monday, January 09, 2006 7:39 PM
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Email: mkamrath-at-nalco.com Name: Mike Kamrath
Organization: Nalco Co., Naperville IL
Title-Subject: [Filtered] Colloidal Silica Particle Size Distribution by TEM
Question: I was looking for help with a problem we have related to particle size distributions (PSD) with colloidal silicas as measured by TEM. We normally dilute a colloidal silica solution in an aqueous solution containing a few drops of nonionic surfactant to help disperse the particles (5-100 nm). After placing a drop on a copper grid (Formvar) we dry it at 100 oC for 10 minutes and place in the TEM for PSD. We are trying to automate the process with counting software but the software has problems identifying individual particles when they are touching one another. I have two questions related to this: 1) Does anyone know of software capable of easily separating touching particles and counting individually? We are currently using Image-Pro Plus and I am aware of free software from NIH (Scion) which seems to have similar problems. 2) Does anybody have suggestions for a better solvent system or surfactant that might separate the particles better? Or is the drying process forcing the particles together and would a vacuum drying or other method work better?
A couple of months ago, I repumped in situ my chronically leaky PGT Be-window EDS detector, and ran 50 deg C air into the dewar while re-evacuating using the chamber vacuum via a hose from the sample introduction port of the JEOL 840 on which the detector is mounted.
The re-evacuation worked well, in that the LN2 consumption is now back to normal, but after recooling and switching on the bias again, there was no appreciable low-energy response -- no visible Cu L peak at all!
I realised that grease from the O-ring sliding seal through which the tube of the detector enters the chamber had melted and run down onto the Be window. Cleaning of the window with Freon restored the Cu L intensity, and all was well, until it became obvious that the window was re-oiling again at a much faster rate than ever before.
Because we do quantitative mineral analysis, I keep a good handle on the window condition, evidenced by both Cu L and Na K responses. I have been using this detector for about five years and not found it neccesary to clean the window before, but now the Na response halves in a few weeks. Recleaning with Freon restores the response again.
In an effort to fix this, I have:-
- changed the rotary pump oil - installed an alumina-pellet foreline trap - changed the DP oil (Santovac, but the old charge was still light-colored)
but still the darned window is oiling up.
I'm running out of ideas. It seems that either the chamber atmosphere has become markedly oilier than before, or the detector window is now running colder than before.
My money is on the latter, as it seems too much of a coincidence for some change in the back-streaming performance of the 840 to have occurred at the same time as the repumping of the detector, but I can't see what might have happened.
Anyone got any suggestions?
Happy New Year
rtch
-- Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 Microanalyst Fax : 64 9 3737435 Department of Geology email : r.sims-at-auckland.ac.nz The University of Auckland Private Bag 92019 Auckland New Zealand
==============================Original Headers============================== 16, 26 -- From r.sims-at-auckland.ac.nz Tue Jan 10 19:00:56 2006 16, 26 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) 16, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0B10td7001103 16, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 19:00:56 -0600 16, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id F369034958 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:00:53 +1300 (NZDT) 16, 26 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) 16, 26 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 16, 26 -- with ESMTP id 31308-14 for {microscopy-at-microscopy.com} ; 16, 26 -- Wed, 11 Jan 2006 14:00:46 +1300 (NZDT) 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 29EF235637 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:59:05 +1300 (NZDT) 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 16, 26 -- To: microscopy-at-microscopy.com 16, 26 -- Date: Wed, 11 Jan 2006 13:59:04 +1300 16, 26 -- MIME-Version: 1.0 16, 26 -- Subject: Oil on detector window 16, 26 -- Message-ID: {43C50F28.11970.13D9DFF-at-localhost} 16, 26 -- Priority: normal 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) 16, 26 -- Content-type: text/plain; charset=US-ASCII 16, 26 -- Content-transfer-encoding: 7BIT 16, 26 -- Content-description: Mail message body 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
First, may I ask what you have done to ensure that your support films are hydrophilic? Carbon coated polyvinylformal supports become increasingly hydrophobic with age. We get good results by treating with spectro-grade acetone for 30 min prior to use. We have also used a short plasma-ash in air to improve wettability. Such treatments improves dispersion and reduces drying artifacts.
We use the analySIS Five software from Soft Imaging System for image analysis. This program has a useful separator based upon a watershed algorithm. I have also written a custom watershed module for some applications. We also use a guard frame to eliminate bias from the higher probability that larger particles touch the image boundaries compared to smaller particles. We prefer this approach to the Miles-Lantejoul algorithm. Using an extension to the analySIS Automater we can queue up images from several samples from any of our microscopes and let them run unattended.
We have compared results obtained by simply deleting agglomerates with the results obtained by separation. Even if we use a separator, we typically measure the maximum intrusion distance [this is a custom module we wrote] for each particle and flag those which are not convex as agglomerates. We always keep track of the area fraction of agglomerates and reject analyses where this is too high. Usually we can find preparation conditions to keep the area fraction of agglomerates below 10%.
One of the strengths of the analySIS program is that the macro language is almost a full subset of C and if one needs a computationally-intensive function (like the maximum intrusion distance) it is easy to import a C function from a custom DLL compiled with a good optimizing compiler, like Visual C++.
Disclaimer: I have no financial interest in Soft Imaging System, writing only as a satisfied user.
John R. Minter Eastman Kodak Co. Research Laboratories Foundation Science and Technology Center john.r.minter-at-kodak.com (work) jrminter-at-rochester.rr.com (home)
-----Original Message----- X-from: mkamrath-at-nalco.com [mailto:mkamrath-at-nalco.com] Sent: Tuesday, January 10, 2006 7:28 PM To: jrminter-at-rochester.rr.com
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please copy both mkamrath-at-nalco.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mkamrath-at-nalco.com Name: Mike Kamrath
Organization: Nalco Co., Naperville IL
Title-Subject: [Filtered] Colloidal Silica Particle Size Distribution by TEM
Question: I was looking for help with a problem we have related to particle size distributions (PSD) with colloidal silicas as measured by TEM. We normally dilute a colloidal silica solution in an aqueous solution containing a few drops of nonionic surfactant to help disperse the particles (5-100 nm). After placing a drop on a copper grid (Formvar) we dry it at 100 oC for 10 minutes and place in the TEM for PSD. We are trying to automate the process with counting software but the software has problems identifying individual particles when they are touching one another. I have two questions related to this: 1) Does anyone know of software capable of easily separating touching particles and counting individually? We are currently using Image-Pro Plus and I am aware of free software from NIH (Scion) which seems to have similar problems. 2) Does anybody have suggestions for a better solvent system or surfactant that might separate the particles better? Or is the drying p! rocess forcing the particles together and would a vacuum drying or other method work better?
I am looking for a simple probe-type sonicator that will be used to make holey films.
We have used an old one in the past that is no longer available. It is used to aerate a formvar/glycerol solution sufficiently to make very small bubbles. When a slide is dipped into the solution, the resulting film has holes of a size dependent on the length of time used for the sonication.
Any recommendations welcomed but hopefully I can find one on the less expensive side.
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 6, 21 -- From dsherman-at-purdue.edu Wed Jan 11 08:11:13 2006 6, 21 -- Received: from exchange.purdue.edu (1061exfe03.adpc.purdue.edu [128.210.63.225]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BEBDF0028321 6, 21 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 08:11:13 -0600 6, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.235]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 6, 21 -- Wed, 11 Jan 2006 09:10:23 -0500 6, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ; 6, 21 -- Wed, 11 Jan 2006 14:10:15 +0000 6, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004 6, 21 -- Date: Wed, 11 Jan 2006 09:11:05 -0500 6, 21 -- Subject: Sonicator 6, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 6, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 21 -- Message-ID: {BFEA7C29.C52D%dsherman-at-purdue.edu} 6, 21 -- Thread-Topic: Sonicator 6, 21 -- Thread-Index: AcYWuNwnGsctLIKsEdqMVgARJN08Mg== 6, 21 -- Mime-version: 1.0 6, 21 -- Content-type: text/plain; 6, 21 -- charset="US-ASCII" 6, 21 -- Content-transfer-encoding: 7bit 6, 21 -- X-OriginalArrivalTime: 11 Jan 2006 14:10:23.0149 (UTC) FILETIME=[C3356DD0:01C616B8] ==============================End of - Headers==============================
sue- For what it's worth, and to get in synch with anyone out there trying tests such as i described, last week I repeated my 40 year old MnO4 tests in water on all the liquid resin components, adding 1-2 small drops to 2.5 ml dilute transparent purple MnO4 sol'n (2.5 ml water, 2 drops 2% KMnO4). I found that NMA instantly turns the sol'n to clear brown (guess I'd misremembered that it made a precipitate before), and DMP-30 does so almost as fast. All the other components, including Araldite 506, do the same thing over periods of 5-200 minutes, Araldite 506 clearly slower than Epon 812. Some make a precipitate, some don't. By next day, nothing retains any purple, all test sol'ns are brown. So the lesson is, MnO4 can act eventually destructively on any sections or their components, given time enough. -mike-
} hi mike...thanks for all your help!!! } sue
-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I am looking into acquiring a new field emission SEM which will be used mainly for failure analysis of metals, but also plastics, paper, an occasional drop of grease, and anything else that wanders through the lab (but rarely biological samples). The field emission SEMs that I am considering are the JEOL JSM-7000F, the ZEISS Supra 40 (VP), the Hitachi S-4300SE/N, and the FEI Quanta 600 FEG. For microanalysis systems, I am considering the newest versions from Thermoelectron, Oxford and EDAX. I welcome any comments that you can offer as to the function (or malfunction) of the above instruments and anaylsis systems, as well as possible electro-magnetic interference issues, user interface issues, interface problems between the SEM and microanalysis systems, and the reliability/response time of the service representatives from the above companies.
Thank you very much for your time!
Sincerely,
Robin Moresi General Dynamics - Advanced Information Systems 100 Plastics Ave. Pittsfield, MA 01201
Tel. (413) 662-2889
==============================Original Headers============================== 8, 26 -- From Robin.Moresi-at-gd-ais.com Wed Jan 11 11:21:00 2006 8, 26 -- Received: from mnblin01.mnb.gd-ais.com (mnblin01.mnb.gd-ais.com [206.11.149.28]) 8, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BHKxPT012197 8, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 11:20:59 -0600 8, 26 -- Received: from mnbm01-fes01.ad.gd-ais.com (mnbm01-fes01.mnb.gd-ais.com [160.207.224.15]) 8, 26 -- by mnblin01.mnb.gd-ais.com (8.12.11/8.12.11) with SMTP id k0BHMC0b006944 8, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 11:22:12 -0600 8, 26 -- Received: from MAPF01-MAIL01.ad.gd-ais.com ([166.16.220.104]) by mnbm01-fes01.ad.gd-ais.com with Microsoft SMTPSVC(6.0.3790.1830); 8, 26 -- Wed, 11 Jan 2006 11:21:06 -0600 8, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 26 -- Content-class: urn:content-classes:message 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: text/plain; 8, 26 -- charset="us-ascii" 8, 26 -- Subject: Field Emission SEM question 8, 26 -- Date: Wed, 11 Jan 2006 12:20:47 -0500 8, 26 -- Message-ID: {5DC7DC21AC968A46AEF8265C6D623C15957A70-at-MAPF01-MAIL01.ad.gd-ais.com} 8, 26 -- X-MS-Has-Attach: 8, 26 -- X-MS-TNEF-Correlator: 8, 26 -- Thread-Topic: Field Emission SEM question 8, 26 -- Thread-Index: AcYWzfPqVEoK4BtUSPGMgcimUwgWZgAADOoAAAEzsbA= 8, 26 -- From: "Moresi, Robin T." {Robin.Moresi-at-gd-ais.com} 8, 26 -- To: {microscopy-at-microscopy.com} 8, 26 -- X-OriginalArrivalTime: 11 Jan 2006 17:21:06.0964 (UTC) FILETIME=[68410540:01C616D3] 8, 26 -- Content-Transfer-Encoding: 8bit 8, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0BHKxPT012197 ==============================End of - Headers==============================
I am looking for a manual for a SORVALL MT2-B ultra microtome. I will be happy to pay for copying and shipping costs, but would greatly appreciate if someone would be willing to copy their manual for me.
Thanks, Elke
Elke Buschbeck Biological Sciences University of Cincinnati Cincinnati, OH elke.buschbeck-at-uc.edu
==============================Original Headers============================== 6, 17 -- From elke.buschbeck-at-uc.edu Wed Jan 11 13:22:43 2006 6, 17 -- Received: from ms-smtp-02-eri0.ohiordc.rr.com (ms-smtp-02-smtplb.ohiordc.rr.com [65.24.5.136]) 6, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BJMeEv026815 6, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:22:42 -0600 6, 17 -- Received: from Mainbrain.uc.edu (cpe-65-27-235-87.cinci.res.rr.com [65.27.235.87]) 6, 17 -- by ms-smtp-02-eri0.ohiordc.rr.com (8.12.10/8.12.7) with ESMTP id k0BJMZg6002796 6, 17 -- for {Microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:22:37 -0500 (EST) 6, 17 -- Message-Id: {6.1.2.0.2.20060111141640.08deb160-at-ucmail8.uc.edu} 6, 17 -- X-Sender: buschbek-at-ucmail8.uc.edu 6, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 17 -- Date: Wed, 11 Jan 2006 14:22:35 -0500 6, 17 -- To: Microscopy-at-microscopy.com 6, 17 -- From: Elke Buschbeck {elke.buschbeck-at-uc.edu} 6, 17 -- Subject: Need manual for Sorvall MT2-B ultra microtome 6, 17 -- Mime-Version: 1.0 6, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 17 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
What has happened to you has happened to many before you!
Once you reach the point of contaminating your window with RP fluid it will have contaminated the complete vacuum system. Two solutions. EITHER take everything apart and clean it, yes ALL the vacuum lines and column liners. OR use a hair drier to bake the pumping lines to drive off the contamination as best as possible, as well as cleaning the parts you are easily able to reach.
Many times in my service technician career I have been forced to take this route. It puts you out of action for a day or so whilst the system recovers but in the main it seems to be a cure. BUT if you do not tackle the fore line trap idea it will happen all over again - our chemical test said it was RP fluid by the way.
Good luck
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7802 966067 Web www.emcourses.com
----- Original Message ----- X-from: {r.sims-at-auckland.ac.nz} To: {protrain-at-emcourses.com} Sent: Wednesday, January 11, 2006 1:02 AM
Will the person that wanted the MT2-B manual please contact me. I found the one I was looking for after I deleted your post.
Mannie Steglich msteglic-at-mdanderson.org
==============================Original Headers============================== 2, 15 -- From msteglic-at-mdanderson.org Wed Jan 11 15:43:11 2006 2, 15 -- Received: from UTM-MAIL04A.mdacc.tmc.edu (utmlnmail04nt.mdacc.tmc.edu [143.111.84.152]) 2, 15 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BLhB5n014618 2, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 11 Jan 2006 15:43:11 -0600 2, 15 -- To: Microscopy-at-MSA.Microscopy.Com 2, 15 -- Subject: MT2-B Manual 2, 15 -- MIME-Version: 1.0 2, 15 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 2, 15 -- Message-ID: {OF72D353B1.75A3682D-ON862570F3.007713B0-862570F3.00774DE6-at-mdacc.tmc.edu} 2, 15 -- From: msteglic-at-mdanderson.org 2, 15 -- Date: Wed, 11 Jan 2006 15:42:54 -0600 2, 15 -- X-MIMETrack: Serialize by Router on UTM-MAIL04A/HOU/UTMDACC(Release 5.0.11 |July 24, 2002) at 2, 15 -- 01/11/2006 03:42:58 PM, 2, 15 -- Serialize complete at 01/11/2006 03:42:58 PM 2, 15 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Many thanks for the replies and suggestions so far, however, please note that this is a new condition.
For the past five years the oiling has not been a problem, and it has started to occur only since the repumping operation described below in tedious detail.
There have been no changes to the system apart from the three steps taken, one at a time, in an attempt to fix the problem (changing the RP oil; installing the foreline trap; and changing the DP oil), and they have made no difference.
I think it would be too much of a coincidence for the cause of the oiling to be unrelated to the repumping operation, so I think that complex cures such as installing LN2 traps, plasma cleaning, complete stripping and cleaning, etc, do not address the question, which is "what has changed to accelerate the detector window oiling so greatly, and how can I get back to the previous state?"
I had thought that perhaps a drop of melted grease might have dripped into the DP, and be decomposing in the Santovac, which propelled me to change the Santovac, but I now realise that this is very unlikely to be the case, as the DP is not directly beneath the sample chamber, and even if it were, such a drop of molten grease would not make it through the water-cooled baffle above the DP. Plus, of course, the fact that very thoroughly cleaning both of the DPs and both of their water-cooled baffles has made no difference at all to the problem.
Incidentally, the Santovac, after five years' continuous operation, was only a pale straw color, boy, that stuff certainly is stable, isn't it?
I feel fairly sure that there is a simple solution to my problem, waiting out there to be noticed, so please keep the suggestions coming.
cheers
rtch
On 10 Jan 2006 at 19:02, r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Here's a problem for the New Year: } } A couple of months ago, I repumped in situ my chronically leaky PGT Be-window EDS } detector, and ran 50 deg C air into the dewar while re-evacuating using the chamber } vacuum via a hose from the sample introduction port of the JEOL 840 on which the } detector is mounted. } } The re-evacuation worked well, in that the LN2 consumption is now back to normal, but } after recooling and switching on the bias again, there was no appreciable low-energy } response -- no visible Cu L peak at all! } } I realised that grease from the O-ring sliding seal through which the tube of the detector } enters the chamber had melted and run down onto the Be window. Cleaning of the } window with Freon restored the Cu L intensity, and all was well, until it became obvious } that the window was re-oiling again at a much faster rate than ever before. } } Because we do quantitative mineral analysis, I keep a good handle on the window } condition, evidenced by both Cu L and Na K responses. I have been using this detector } for about five years and not found it neccesary to clean the window before, but now the } Na response halves in a few weeks. Recleaning with Freon restores the response } again. } } In an effort to fix this, I have:- } } - changed the rotary pump oil } - installed an alumina-pellet foreline trap } - changed the DP oil (Santovac, but the old charge was still light-colored) } } but still the darned window is oiling up. } } I'm running out of ideas. It seems that either the chamber atmosphere has become } markedly oilier than before, or the detector window is now running colder than before. } } My money is on the latter, as it seems too much of a coincidence for some change in } the back-streaming performance of the 840 to have occurred at the same time as the } repumping of the detector, but I can't see what might have happened. } } Anyone got any suggestions? } } Happy New Year } } rtch } } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 16, 26 -- From r.sims-at-auckland.ac.nz Tue Jan 10 19:00:56 2006 } 16, 26 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) } 16, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0B10td7001103 } 16, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 19:00:56 -0600 } 16, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id F369034958 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:00:53 +1300 (NZDT) } 16, 26 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) } 16, 26 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) } 16, 26 -- with ESMTP id 31308-14 for {microscopy-at-microscopy.com} ; } 16, 26 -- Wed, 11 Jan 2006 14:00:46 +1300 (NZDT) } 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 29EF235637 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:59:05 +1300 (NZDT) } 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 16, 26 -- To: microscopy-at-microscopy.com } 16, 26 -- Date: Wed, 11 Jan 2006 13:59:04 +1300 } 16, 26 -- MIME-Version: 1.0 } 16, 26 -- Subject: Oil on detector window } 16, 26 -- Message-ID: {43C50F28.11970.13D9DFF-at-localhost} } 16, 26 -- Priority: normal } 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) } 16, 26 -- Content-type: text/plain; charset=US-ASCII } 16, 26 -- Content-transfer-encoding: 7BIT } 16, 26 -- Content-description: Mail message body } 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers==============================
==============================Original Headers============================== 15, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 11 17:09:46 2006 15, 27 -- Received: from groucho.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11]) 15, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BN9jBt024828 15, 27 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 17:09:46 -0600 15, 27 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 3333B352E7 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from groucho.itss.auckland.ac.nz ([127.0.0.1]) 15, 27 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 27 -- with ESMTP id 13091-10 for {microscopy-at-microscopy.com} ; 15, 27 -- Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 0E039343ED 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 27 -- To: microscopy-at-microscopy.com 15, 27 -- Date: Thu, 12 Jan 2006 12:12:02 +1300 15, 27 -- MIME-Version: 1.0 15, 27 -- Subject: More about oil on detector window 15, 27 -- Message-ID: {43C64792.29976.BC74A1-at-localhost} 15, 27 -- Priority: normal 15, 27 -- In-reply-to: {200601110102.k0B12645002985-at-ns.microscopy.com} 15, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 15, 27 -- Content-type: text/plain; charset=US-ASCII 15, 27 -- Content-transfer-encoding: 7BIT 15, 27 -- Content-description: Mail message body 15, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
Dear Ritch, Does the snout of the EDS detector feel cool to the touch, when you vent the chamber? (Use the back of your hand to test it.) I had one detector that oiled very badly and it felt cool to the touch, so it acted as an oil trap for the chamber. I sent that detector to be completely rebuilt as a light element, high resolution detector and that problem has vanished, so it was a condition of the detector, not the SEM, since the SEM was not touched. It may be that the insulation between the liquid nitrogen or the cooled components and the snout of the detector has changed with your warm-up and re-pump. I am wondering if the copper braid that conducts the cool from the dewar to the nose of the detector has moved or is touching something. None of this helps you in any way. You can clean the Be-window detector with a trickle of pure ethanol or iso-propanol in the SEM, if you can get at it. ----- Original Message ----- X-from: {r.sims-at-auckland.ac.nz} To: {mager-at-interchange.ubc.ca} Sent: Wednesday, January 11, 2006 3:13 PM
Ritch, This is a long shot and you probably would have noticed the symptoms, but here goes:
I had one 840 that kept being "burped" first thing in the morning, but not after that. The key to finding the problem was that the LEDs on the vacuum control all cycled correctly, but it took me a while (since it only happened once a day) to notice that the actual valve operation for V2 (at the base of the second DP) was delayed just long enough to burp the DPs. Turns out the solenoid valve that operated V2 had Apiezon in it (a lot) and after sitting overnight would stick for about 2 seconds, only milliseconds longer than the delay for V5 to rough the load lock. This problem had been intermittent for years before I took on the service contract, but things work fine, now.
Perhaps you have a similar timing problem. Of course, the most obvious thing was that the DPs dumped and you don't seem to have had anything that severe happening.
Another thought: when you cleaned the DPs, did you also remove and clean the ballast tank located between them? Perhaps it has become too contaminated and is now backstreaming since there is only one DP between it and the chamber.
Do you have any additional gauging to monitor the actual pressure in the chamber? There might be some clues there, also. I'm thinking along the lines of a slightly leaky V4.
One last thought: Is the water flowing in the correct direction? An accidental reversal puts warm water running through the water baffles and virtually always results in oil on the EDS detector.
Best of luck, Ken Converse
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: Wednesday, January 11, 2006 6:12 PM To: kenconverse-at-qualityimages.biz
Many thanks for the replies and suggestions so far, however, please note that this is a new condition.
For the past five years the oiling has not been a problem, and it has started to occur only since the repumping operation described below in tedious detail.
There have been no changes to the system apart from the three steps taken, one at a time, in an attempt to fix the problem (changing the RP oil; installing the foreline trap; and changing the DP oil), and they have made no difference.
I think it would be too much of a coincidence for the cause of the oiling to be unrelated to the repumping operation, so I think that complex cures such as installing LN2 traps, plasma cleaning, complete stripping and cleaning, etc, do not address the question, which is "what has changed to accelerate the detector window oiling so greatly, and how can I get back to the previous state?"
I had thought that perhaps a drop of melted grease might have dripped into the DP, and be decomposing in the Santovac, which propelled me to change the Santovac, but I now realise that this is very unlikely to be the case, as the DP is not directly beneath the sample chamber, and even if it were, such a drop of molten grease would not make it through the water-cooled baffle above the DP. Plus, of course, the fact that very thoroughly cleaning both of the DPs and both of their water-cooled baffles has made no difference at all to the problem.
Incidentally, the Santovac, after five years' continuous operation, was only a pale straw color, boy, that stuff certainly is stable, isn't it?
I feel fairly sure that there is a simple solution to my problem, waiting out there to be noticed, so please keep the suggestions coming.
cheers
rtch
On 10 Jan 2006 at 19:02, r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Here's a problem for the New Year: } } A couple of months ago, I repumped in situ my chronically leaky PGT Be-window EDS } detector, and ran 50 deg C air into the dewar while re-evacuating using the chamber } vacuum via a hose from the sample introduction port of the JEOL 840 on which the } detector is mounted. } } The re-evacuation worked well, in that the LN2 consumption is now back to normal, but } after recooling and switching on the bias again, there was no appreciable low-energy } response -- no visible Cu L peak at all! } } I realised that grease from the O-ring sliding seal through which the tube of the detector } enters the chamber had melted and run down onto the Be window. Cleaning of the } window with Freon restored the Cu L intensity, and all was well, until it became obvious } that the window was re-oiling again at a much faster rate than ever before. } } Because we do quantitative mineral analysis, I keep a good handle on the window } condition, evidenced by both Cu L and Na K responses. I have been using this detector } for about five years and not found it neccesary to clean the window before, but now the } Na response halves in a few weeks. Recleaning with Freon restores the response } again. } } In an effort to fix this, I have:- } } - changed the rotary pump oil } - installed an alumina-pellet foreline trap } - changed the DP oil (Santovac, but the old charge was still light-colored) } } but still the darned window is oiling up. } } I'm running out of ideas. It seems that either the chamber atmosphere has become } markedly oilier than before, or the detector window is now running colder than before. } } My money is on the latter, as it seems too much of a coincidence for some change in } the back-streaming performance of the 840 to have occurred at the same time as the } repumping of the detector, but I can't see what might have happened. } } Anyone got any suggestions? } } Happy New Year } } rtch } } } -- } Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713 } Microanalyst Fax : 64 9 3737435 } Department of Geology email : r.sims-at-auckland.ac.nz } The University of Auckland } Private Bag 92019 } Auckland } New Zealand } } } ==============================Original Headers============================== } 16, 26 -- From r.sims-at-auckland.ac.nz Tue Jan 10 19:00:56 2006 } 16, 26 -- Received: from chico.itss.auckland.ac.nz (chico.itss.auckland.ac.nz [130.216.190.12]) } 16, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0B10td7001103 } 16, 26 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2006 19:00:56 -0600 } 16, 26 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id F369034958 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 14:00:53 +1300 (NZDT) } 16, 26 -- Received: from chico.itss.auckland.ac.nz ([127.0.0.1]) } 16, 26 -- by localhost (smtpb.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) } 16, 26 -- with ESMTP id 31308-14 for {microscopy-at-microscopy.com} ; } 16, 26 -- Wed, 11 Jan 2006 14:00:46 +1300 (NZDT) } 16, 26 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) } 16, 26 -- by chico.itss.auckland.ac.nz (Postfix) with ESMTP id 29EF235637 } 16, 26 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 13:59:05 +1300 (NZDT) } 16, 26 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} } 16, 26 -- To: microscopy-at-microscopy.com } 16, 26 -- Date: Wed, 11 Jan 2006 13:59:04 +1300 } 16, 26 -- MIME-Version: 1.0 } 16, 26 -- Subject: Oil on detector window } 16, 26 -- Message-ID: {43C50F28.11970.13D9DFF-at-localhost} } 16, 26 -- Priority: normal } 16, 26 -- X-mailer: Pegasus Mail for Windows (4.21c) } 16, 26 -- Content-type: text/plain; charset=US-ASCII } 16, 26 -- Content-transfer-encoding: 7BIT } 16, 26 -- Content-description: Mail message body } 16, 26 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz } ==============================End of - Headers==============================
==============================Original Headers============================== 15, 27 -- From r.sims-at-auckland.ac.nz Wed Jan 11 17:09:46 2006 15, 27 -- Received: from groucho.itss.auckland.ac.nz (groucho.itss.auckland.ac.nz [130.216.190.11]) 15, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0BN9jBt024828 15, 27 -- for {microscopy-at-microscopy.com} ; Wed, 11 Jan 2006 17:09:46 -0600 15, 27 -- Received: from localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 3333B352E7 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from groucho.itss.auckland.ac.nz ([127.0.0.1]) 15, 27 -- by localhost (smtpa.itss.auckland.ac.nz [127.0.0.1]) (amavisd-new, port 10024) 15, 27 -- with ESMTP id 13091-10 for {microscopy-at-microscopy.com} ; 15, 27 -- Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- Received: from rs (r.sims.glg.auckland.ac.nz [130.216.59.18]) 15, 27 -- by groucho.itss.auckland.ac.nz (Postfix) with ESMTP id 0E039343ED 15, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 12:09:44 +1300 (NZDT) 15, 27 -- From: "Ritchie Sims" {r.sims-at-auckland.ac.nz} 15, 27 -- To: microscopy-at-microscopy.com 15, 27 -- Date: Thu, 12 Jan 2006 12:12:02 +1300 15, 27 -- MIME-Version: 1.0 15, 27 -- Subject: More about oil on detector window 15, 27 -- Message-ID: {43C64792.29976.BC74A1-at-localhost} 15, 27 -- Priority: normal 15, 27 -- In-reply-to: {200601110102.k0B12645002985-at-ns.microscopy.com} 15, 27 -- X-mailer: Pegasus Mail for Windows (4.21c) 15, 27 -- Content-type: text/plain; charset=US-ASCII 15, 27 -- Content-transfer-encoding: 7BIT 15, 27 -- Content-description: Mail message body 15, 27 -- X-Virus-Scanned: by amavisd-new at mailhost.auckland.ac.nz ==============================End of - Headers==============================
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==============================Original Headers============================== 36, 23 -- From kenconverse-at-qualityimages.biz Thu Jan 12 09:30:50 2006 36, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 36, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CFUnmn022474 36, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 12 Jan 2006 09:30:49 -0600 36, 23 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 36, 23 -- (SMTPD32-8.05) id A61691000C4; Thu, 12 Jan 2006 07:30:30 -0800 36, 23 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 36, 23 -- To: {r.sims-at-auckland.ac.nz} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 36, 23 -- Subject: RE: [Microscopy] More about oil on detector window 36, 23 -- Date: Thu, 12 Jan 2006 10:30:26 -0500 36, 23 -- Message-ID: {003701c6178d$2273cd80$6501a8c0-at-Ken} 36, 23 -- MIME-Version: 1.0 36, 23 -- Content-Type: text/plain; 36, 23 -- charset="us-ascii" 36, 23 -- X-Priority: 3 (Normal) 36, 23 -- X-MSMail-Priority: Normal 36, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 36, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 36, 23 -- In-Reply-To: {200601112312.k0BNCQVD030081-at-ns.microscopy.com} 36, 23 -- Importance: Normal 36, 23 -- X-IMSTrailer: __IMail_7__ 36, 23 -- Content-Transfer-Encoding: 8bit 36, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0CFUnmn022474 ==============================End of - Headers==============================
Transmission Electron Microscopy (http://www.collegeofmicroscopy.com/courses/17.asp)
Both are hands-on, introductory level courses taught using the latest equipment available. Please follow the links provided for further details and registration information.
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Thu Jan 12 14:13:16 2006 11, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 11, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CKDGdt003847 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 12 Jan 2006 14:13:16 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 4FC601A800B 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 12 Jan 2006 14:13:17 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Thu, 12 Jan 2006 14:13:17 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 11, 27 -- Subject: Short Course Announcement: Scientific Imaging, Transmission Electron Microscopy 11, 27 -- Date: Thu, 12 Jan 2006 14:13:06 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C30F7-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Short Course Announcement: Scientific Imaging, Transmission Electron Microscopy 11, 27 -- Thread-Index: AcYXtJk/0FDZxb4QTf2pOACZsYbNJQ== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0CKDGdt003847 ==============================End of - Headers==============================
Does anyone know of a reasonable alternative to the venerable Zero Stat static taming guns? We use them often but there's just got to be a better way. I used to smuggle squirt guns onto the school bus that were made better (the good old days), and they didn't cost over $100 for about $1 worth of components.
Thanks and Happy New Year to all.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Thu Jan 12 16:59:22 2006 9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CMxMkH015190 9, 23 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 16:59:22 -0600 9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Thu, 12 Jan 2006 16:59:22 -0600 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: Static guns? 9, 23 -- Date: Thu, 12 Jan 2006 16:59:21 -0600 9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849CB7-at-UM-EMAIL09.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: Static guns? 9, 23 -- Thread-Index: AcYXy9ND13Q0Mk2eRQC7DwCisMtGXA== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 12 Jan 2006 22:59:22.0766 (UTC) FILETIME=[D3E862E0:01C617CB] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0CMxMkH015190 ==============================End of - Headers==============================
Job Opening #B005608; Lab Technician/Engineer at IBM
There is an opening at the Lab Technician/Engineer level within the Materials Characterization and Analysis Group in the Research Division of IBM at the Almaden Research Center in San Jose, California. The job is a long term (three years) supplemental position with benefits. A B.S. in material science or equivalent fields, or successful completion of a two year college program plus experience, is required.
This position involves technical support in an advanced electron microscopy laboratory which emphasizes transmission electron microscopy. The technician works as a team member with professional (Ph.D.) microscopists and other scientists in a research laboratory. Duties involve primarily sample preparation methods including the conventional grinding, polishing, and ion milling techniques, focused ion beam (FIB ) method, as well as Microtome, etc. In addition, the candidate must be able to operate and perform routine maintenance on specimen preparation equipment, interact with customers (professional scientists), and prepare reports. The candidate must be able to work independently within set priorities, to keep abreast of new advances, and to interact smoothly within a team.
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Dr. Philip M. Rice IBM Research Division Almaden Research Center 650 Harry Road, K19B/D1 tel: (408) 927-1442 fax: (408) 927-2100 email: pmrice-at-almaden.ibm.com
Dr. Philip M. Rice IBM Research Division Almaden Research Center 650 Harry Road, K19B/D1 San Jose, CA 95120-6099 tel: (408) 927-1442 fax: (408) 927-2100 e-mail: pmrice-at-almaden.ibm.com
==============================Original Headers============================== 10, 26 -- From pmrice-at-almaden.ibm.com Thu Jan 12 17:19:37 2006 10, 26 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 10, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0CNJaZ0024520 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 17:19:36 -0600 10, 26 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 10, 26 -- by e6.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id k0CNJZhi023925 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 10, 26 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id k0CNJZBB128016 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 10, 26 -- by d01av03.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k0CNJZ9b030875 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Received: from d01ml605.pok.ibm.com (d01ml605.pok.ibm.com [9.56.227.91]) 10, 26 -- by d01av03.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k0CNJZ8m030872 10, 26 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2006 18:19:35 -0500 10, 26 -- Subject: TEM - Job opening for a TEM Specimen Preparation Lab Technician 10, 26 -- To: microscopy-at-microscopy.com 10, 26 -- X-Mailer: Lotus Notes Release 6.0.2CF1 June 9, 2003 10, 26 -- Message-ID: {OF50DE3E5B.2E1B3545-ON882570F4.007FA844-882570F4.008020F8-at-us.ibm.com} 10, 26 -- From: Philip Rice {pmrice-at-almaden.ibm.com} 10, 26 -- Date: Thu, 12 Jan 2006 15:19:30 -0800 10, 26 -- X-MIMETrack: Serialize by Router on D01ML605/01/M/IBM(Release 7.0HF90 | November 16, 2005) at 10, 26 -- 01/12/2006 18:19:34 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
I am trying to permeabilize some Haemophilus influenzae fixed in 2% paraformaldehyde + 0.2% glutaraldehyde so I can immunostain an intracellular protein. My first attempts to permeabilize with Triton X-100 were surprising unsuccessful. I tried both literature searches and googling this topic but a search of "bacteria" + "permeabilization" pulls up thousands on non-germane citations. I am interested in comments from anybody with expertise in permeabilization, especially in regard to the bacterial target. Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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Email: aetmicro-at-optonline.net Name: Andrew Thelian
Title-Subject: [Filtered] Philips EM 300 beam issue
Question: Hi All,
I have hit yet another bump in the road...
I was having a brightness issue last week that i thought might be resolved with cleaning the wehnelt assembly...
Now when i energize the filament i get a very small circle on the view screen...it doesnt appear green it looks like a dim reddish color...it also seems to respond to turning the filament knob...
the condenser and deflection knobs dont cause any change...
I have removed all apertures... to see if there was a beam any where ... but no luck...
This Question was submitted to Ask-A-Microscopist by (dustin.adams-at-xerox.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 12, 2006 at 17:11:24 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both dustin.adams-at-xerox.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Question: Hello, I am going to be training soon to become a SEM microscopist for my company, and was curious to see what the salary amounts are for a typical person in my position. I have tried to look for this info online, but wasn't really able to find anything. If someone could provide that information or point me in the right direction; I would greatly appreciate it.
What you see is the light from filament, not e-beam. Whenever electron gun was disturbed, in your case for Wehnelt cleaning, gun tilt must be re-aligned, and perhaps gun shift too. Both these alignments are mechanical on Phil. EM300. Users manual will help if TEM is functioning properly.
Green light or not, you must be able to tell whether HT is present by watching emission current, and emission meter behavior: a) when HT turned on/off; b) emission setting changed while HT is on; c) and HV setting changed while HT is on.
If HT is present, if filament glows, and if emission current behaves normally, do the following: a) select lowest magnification - I believe it is SC (scan) position; b) move all apertures from the beam pass; c) if no beam yet, turn lens currents off one by one and you must see the beam. Be careful not to burn the screen (keep filament under-saturated). Just turning off C1 and C2 will likely bring the beam back, and then work from that point on.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {aetmicro-at-optonline.net} To: {vitalylazar-at-att.net} Sent: Thursday, January 12, 2006 9:23 PM
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Email: secr-at-eurmicsoc.org Name: Nick Schryvers
Organization: EMS
Title-Subject: [Filtered] EMS scholarships for IMC16 Msg 2006004
Question: The European Microscopy Society is pleased to be able to offer 4 scholarships of 500 Euro each to young researchers in support of attending the 16th International Microscopy Congress (http://www.imc16.jp/) in Sapporo, Japan, from Sep. 3 till Sep. 8, 2006. Applications should be sent to the EMS secretary (secr-at-eurmicsoc.org) and include a CV and list of publications and copies of the submitted abstract(s) at IMC16. Proof of acceptance of the abstract(s) should follow as soon as these are available.
Deadline of the applications is February 1, 2006 (same date as abstract submission for IMC16)
Only EMS members are eligible and priority will be given to people under the age of 35.
This support was made possible through a generous offer from JEOL Europe (http://www.jeoleuro.com/).
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both secr-at-eurmicsoc.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: secr-at-eurmicsoc.org Name: Nick Schryvers
Organization: EMS
Title-Subject: [Filtered] Royal Microscopical Society ( RMS) Spring School in Electron Microscopy
Royal Microscopical Society ( RMS) Spring School in Electron Microscopy 27-31 March 2006 University of Leeds, UK. Course Organiser: Prof. Rik Brydson
The RMS School consists of a number of parallel courses covering most aspects of electron microscopy in both the physical and life sciences. Students can attend either 4 or 5 days - the 5 day course includes a day of principles of EM lectures for beginners or as a refresher. During the week, students will have the opportunity to choose to attend lectures and workshops from either course, to tailor it to their specific needs.
'All those involved in the organisation of this event are to be congratulated. The complexity of running a school of electron microscopy covering both life and materials sciences has been surpassed with remarkable success with participants, speakers and organisers equally satisfied with the end results.'
Pedro Costa - Participant Spring School in EM 2005
For more information or to book for this course please visit the RMS website: {http://www.rms.org.uk/event_em_school.shtml} http://www.rms.org.uk/event_em_school.shtml
Victoria Lee Conference Manager Royal Microscopical Society 37/38 St Clements, Oxford OX4 1AJ
I always used a sealed Polonium-210 beta source for static problems - Nuclepore Corp used to make a Polonium-210 (sealed in beads) metal strip that had an open ladder arrangement at the top that you simply placed the Nuclepore filters on. It really reduced static. The only downside was the half-life of a few months that meant after a year or so you had to buy another. We also had Zero-Stat gun, but that wasn't used as it simply didn't work. The Nuclepore device seems to have been dropped now, but you can buy a brush that has Polonium-210 trapped within the bristles that should work on similar lines (NRD - StaticMaster Anti-Static Brush - 1" or 3") :
I know that photographers use them to reduce static on film. I've never tried this specific brush though as I have now moved out of this field. Our histologist used something similar on microtome surfaces when sectioning I remember.
Also have a look at ionisers like : http://www.etherton.co.uk/index.html http://www.2spi.com/catalog/photo/antistat.html
Alternatively you can spray a conducting coating onto the plastic to eliminate static (e.g. the Duron anti-static spray sold by Agar Scientific that "even allows uncoated samples to be viewed under 'low resolution' in an SEM").
There's also the anti-static wrist-strap (or just touching the metal earth of a plugged in PC case or wall socket screw head). Plus breathing on the plastic really removes the static (or earthing the material if it conducts), but that suits plastic record player Perspex covers more than scientific specimens. Keeping the room %RH constant and not going too low will also help. Some labs have a full antistatic grounding system with furniture, mats etc.. all 'grounded' with controlled %RH and temperatures, but we got by with antistatic mats, higher humidity and the polonium-210. We avoided using gloves as much as possible, using fine metal forceps for handling filters (I don't think we bothered with 'antistatic' forceps). Antistatic gloves weren't much use as we needed disposable ones. We used very thin sensitive clear plastic gloves that were probably something like Polyethylene, although they probably did have some static problems. Our Nuclepore filter samples were generally glassfibres recovered from lungs (fibre durability + toxicology studies) or aerosol particles, and obviously when using aqueous samples static was only a problem with the clean filter prior to filtration or after drying.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {TindallR-at-missouri.edu} To: {keith.morris-at-ucl.ac.uk} Sent: Thursday, January 12, 2006 11:04 PM
I'll send you a PDF of the salary survey article that appeared in Microscopy Today last year. Also, if you are going to be a microscopist (congratulations!) you should sign up for a free subscription to Microscopy Today at http://www.microscopy-today.com.
Ron Anderson, Editor
dustin.adams-at-xerox.com wrote:
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==============================Original Headers============================== 6, 20 -- From microscopytoday-at-tampabay.rr.com Fri Jan 13 08:32:19 2006 6, 20 -- Received: from ms-smtp-03.tampabay.rr.com (ms-smtp-03-smtplb.tampabay.rr.com [65.32.5.133]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DEWIuY013660 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jan 2006 08:32:18 -0600 6, 20 -- Received: from [127.0.0.1] (rrcs-24-73-73-214.se.biz.rr.com [24.73.73.214]) 6, 20 -- by ms-smtp-03.tampabay.rr.com (8.13.4/8.13.4) with ESMTP id k0DEWDul022696; 6, 20 -- Fri, 13 Jan 2006 09:32:17 -0500 (EST) 6, 20 -- Message-ID: {43C7B9EC.8030201-at-tampabay.rr.com} 6, 20 -- Date: Fri, 13 Jan 2006 09:32:12 -0500 6, 20 -- From: Microscopy Today {microscopytoday-at-tampabay.rr.com} 6, 20 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 20 -- X-Accept-Language: en-us, en 6, 20 -- MIME-Version: 1.0 6, 20 -- To: dustin.adams-at-xerox.com, Listserver {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM microscopist salaries 6, 20 -- References: {200601130224.k0D2Oc6V017874-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200601130224.k0D2Oc6V017874-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Virus-Scanned: Symantec AntiVirus Scan Engine ==============================End of - Headers==============================
Thanks for all the suggestions on static control! I learned about some new things to try for general use. I had forgotten about the polonium strip StaticMaster brushes from my days locked in a photo darkroom, but this may be a good alternative to these outrageously overpriced Zero Stat guns that don't work nearly as well as they did a few years ago.
Those StaticMaster brushes used to strike fear into us when we read the disposal instructions, which were something like "seal this strip inside a deep, deep mine and don't go anywhere near it for 5000 years", although we could use them in the darkroom with no special protection. They did work, though, except you couldn't spark your co-workers with them and make them squeal.
By the way, our most common use for these guns is somewhat trivial: getting grids down off the lid of the petri dish, where static electricity glues them, especially in winter. (Tamara, I agree with you about the static in New Mexico---I still have scars from getting zapped every time I'd get out of my car in Las Cruces!). We use the Diatome de-ionizer for microtomy, which works well.
Thanks again!
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
==============================Original Headers============================== 11, 23 -- From TindallR-at-missouri.edu Fri Jan 13 08:50:02 2006 11, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 11, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DEo1kl023115 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 08:50:01 -0600 11, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 11, 23 -- Fri, 13 Jan 2006 08:49:59 -0600 11, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 23 -- Content-class: urn:content-classes:message 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- charset="us-ascii" 11, 23 -- Subject: Static! 11, 23 -- Date: Fri, 13 Jan 2006 08:49:58 -0600 11, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849CB9-at-UM-EMAIL09.um.umsystem.edu} 11, 23 -- X-MS-Has-Attach: 11, 23 -- X-MS-TNEF-Correlator: 11, 23 -- Thread-Topic: Static! 11, 23 -- Thread-Index: AcYYUKGubaVRxOz9TI+TEu+84a1Tdg== 11, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- X-OriginalArrivalTime: 13 Jan 2006 14:49:59.0341 (UTC) FILETIME=[A05B0DD0:01C61850] 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DEo1kl023115 ==============================End of - Headers==============================
You want static??!!! Try Manitoba in February. When it's -30C there's no such thing as moisture in the air. But then, not even Phil was willing to try Manitoba in June/July, even for real beer ;-) .
Great time to make formvar grids, though.
paul
} Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } e-mail: paul_hazelton-at-umanitoba.ca } Phone:204-789-3313 } Pager:204-931-9354 } Cell:204-781-1502 } Fax:204-789-3926
==============================Original Headers============================== 12, 20 -- From paul_hazelton-at-umanitoba.ca Fri Jan 13 09:59:12 2006 12, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 12, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DFwxMP001111 12, 20 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 09:59:01 -0600 12, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 12, 20 -- (authenticated bits=0) 12, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k0DFwtYb015625; 12, 20 -- Fri, 13 Jan 2006 09:58:56 -0600 (CST) 12, 20 -- Message-ID: {43C7CE3B.4080709-at-umanitoba.ca} 12, 20 -- Date: Fri, 13 Jan 2006 09:58:51 -0600 12, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 12, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 12, 20 -- X-Accept-Language: en-us, en 12, 20 -- MIME-Version: 1.0 12, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 12, 20 -- Subject: Re: [Microscopy] Static! 12, 20 -- References: {200601131453.k0DErNj8026940-at-ns.microscopy.com} 12, 20 -- In-Reply-To: {200601131453.k0DErNj8026940-at-ns.microscopy.com} 12, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 12, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Definition of milli-second is the amount of time between hitting send and realizing there is an error, da? Of course I meant phospholipid, not lipoprotein when mentioning the outer and cytoplasmic membranes. Mark it up to trying to do e-mail when the brain is turned off. Should Ron Anderson decide that this is a train worth following for Microscopy Today and want to use my response to you, perhaps he could make the correction, please??!!
Paul
} Paul R. Hazelton, PhD } Electron Microscope Unit } University of Manitoba } Department of Medical Microbiology } 531 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 0W3 } e-mail: paul_hazelton-at-umanitoba.ca } Phone:204-789-3313 } Pager:204-931-9354 } Cell:204-781-1502 } Fax:204-789-3926
==============================Original Headers============================== 10, 20 -- From paul_hazelton-at-umanitoba.ca Fri Jan 13 09:59:41 2006 10, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 10, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DFxdMb001278 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 09:59:40 -0600 10, 20 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 10, 20 -- (authenticated bits=0) 10, 20 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k0DFxcsx015957 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 09:59:38 -0600 (CST) 10, 20 -- Message-ID: {43C7CE65.7000006-at-umanitoba.ca} 10, 20 -- Date: Fri, 13 Jan 2006 09:59:33 -0600 10, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 10, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 10, 20 -- X-Accept-Language: en-us, en 10, 20 -- MIME-Version: 1.0 10, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 10, 20 -- Subject: Re: [Microscopy] bacterial permeabilization - further 10, 20 -- References: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 10, 20 -- In-Reply-To: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 10, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 10, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Very interesting question. Made more interesting to me by the fact that I was discussing this same topic this afternoon, only more generically, and certainly not in reference to H. influenza. The problem you are faced with is bacterial structure. H. influenza is a Gram negative microorganism. Gram negative cells have two lipoprotein layers - the outer and cytoplasmic membranes. In addition, you have a layer of peptidoglycan between the outer and cytoplasmic membranes. To make it even more fun, there is frequently a lypopolysaccharide layer around the whole thing. You have to penetrate the LPS, permeabilize the outer membrane, traverse the periplasmic space (the volume between the outer and cytoplasmic membranes), cross the peptidoglycan layer and then penetrate the inner membrane, all with gentle treatment to allow the membranes to reorganize after removal of the detergent you use for permeabilization. What it adds up to is that permeabilization will be difficult at best, and most likely impossible. Probably the only way to get something reliably into the cytoplasm would be by active transport.
Having said that, knowing what I do about the structure of prokaryotic cells, I confess to having tried to permeabilize and do pre-embedding labeling on E. coli expressing a protein of interest (another gram negative bacterium). Didn't expect it, but tried anyway. Hey, dogma schmogma, give it a try, right? That's how the serendipitous findings occur. Anyway, used triton X, used saponin. Ugly. Did not look good. No luck either way. So logic and dogma were right this time.
At the same time I did LR white embedding on cells from the same broth culture. Included osmium fixation. Went back with metaperiodate followed by hydrogen peroxide etching and then did indirect IEM with 12nm gold for the label. Beautiful preservation. Got highly significant labeling of the expressed protein in the E. coli and in the wild type bacterium from which the protein of interest had been cloned. In fact, the protein of interest was hypothesized to be membrane inserted on the basis of amino acid sequence and the labeling of the wild type cells was primarily associated with the cytoplasmic membrane. In short, I did minor modification to the standard LR white embedding protocol and it worked great. I would give that a try.
I would like to give a reference for the work, but we are still waiting for the student to finish his thesis, not to mention the paper. And he has already left for his post-doc. If you have any questions, call. I would be glad to discuss the procedure I used further.
Paul
==============================Original Headers============================== 9, 21 -- From paul_hazelton-at-umanitoba.ca Fri Jan 13 10:12:07 2006 9, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DGC78e019513 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 10:12:07 -0600 9, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 9, 21 -- (authenticated bits=0) 9, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k0DGC684025968; 9, 21 -- Fri, 13 Jan 2006 10:12:06 -0600 (CST) 9, 21 -- Message-ID: {43C7D151.2010503-at-umanitoba.ca} 9, 21 -- Date: Fri, 13 Jan 2006 10:12:01 -0600 9, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 9, 21 -- X-Accept-Language: en-us, en 9, 21 -- MIME-Version: 1.0 9, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 9, 21 -- Subject: Re: [Microscopy] bacterial permeabilization 9, 21 -- References: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200601130008.k0D08WwZ005845-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DGC78e019513 ==============================End of - Headers==============================
Hey! I would've been happy to go to Manitoba, even for real beer. Just kind of hard when I was busy fending off predatory administraitors. Gave up on that and just moved.
Phil
Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Hi, Dustin
We did a salary survey for the society in conjunction with Microscopy Today late last year. Contact Ron Anderson for details. (see email above).
Hope this is helpful, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 08:25 PM 1/12/2006, dustin.adams-at-xerox.com wrote:
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==============================Original Headers============================== 13, 18 -- From bfoster-at-mme1.com Fri Jan 13 10:57:55 2006 13, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 13, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DGvtFJ005902 13, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 10:57:55 -0600 13, 18 -- Received: (qmail 27399 invoked from network); 13 Jan 2006 11:59:33 -0500 13, 18 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 13, 18 -- by enterprise.5starpro.com with SMTP; 13 Jan 2006 11:59:33 -0500 13, 18 -- Message-Id: {6.1.2.0.0.20060113105646.0273a880-at-mail.mme1.com} 13, 18 -- X-Sender: bfoster-at-mail.mme1.com 13, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 13, 18 -- Date: Fri, 13 Jan 2006 10:57:56 -0600 13, 18 -- To: dustin.adams-at-xerox.com, microscopy-at-microscopy.com 13, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 13, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM microscopist salaries 13, 18 -- In-Reply-To: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 13, 18 -- References: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 13, 18 -- Mime-Version: 1.0 13, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
At 08:25 PM 1/12/2006, dustin.adams-at-xerox.com wrote:
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==============================Original Headers============================== 6, 18 -- From bfoster-at-mme1.com Fri Jan 13 11:03:21 2006 6, 18 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DH3KI6015095 6, 18 -- for {microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 11:03:20 -0600 6, 18 -- Received: (qmail 27830 invoked from network); 13 Jan 2006 12:04:58 -0500 6, 18 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 6, 18 -- by enterprise.5starpro.com with SMTP; 13 Jan 2006 12:04:58 -0500 6, 18 -- Message-Id: {6.1.2.0.0.20060113105606.026f6358-at-mail.mme1.com} 6, 18 -- X-Sender: bfoster-at-mail.mme1.com 6, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 6.1.2.0 6, 18 -- Date: Fri, 13 Jan 2006 11:03:21 -0600 6, 18 -- To: dustin.adams-at-xerox.com, microscopy-at-microscopy.com 6, 18 -- From: Barbara Foster {bfoster-at-mme1.com} 6, 18 -- Subject: Re: [Microscopy] AskAMicroscopist: SEM microscopist salaries 6, 18 -- In-Reply-To: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 6, 18 -- References: {200601130225.k0D2PxKo021933-at-ns.microscopy.com} 6, 18 -- Mime-Version: 1.0 6, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I am looking at getting a deeply cooled (-30 C) high sensitivity, high resolution digital camera. I am considering the following cameras : Hamamatsu Orca-AG Retiga SRV Photometrics CoolSnap HQ or K4
Any comments (public or private) on your experiences would be appreciated.
One source has told me that they see little practical difference in the Orca (cooled to -30) vs the Retiga EXi (cooled to 25 below ambient). Comments about this statement are also welcome.
Thanks, Tom
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
McCrone Associates, Inc., an innovative modern analytical and research laboratory, seeks a highly motivated electron microscopy technician with training in ultramicrotomy for materials science. The successful candidate will support our Electron Optics Group in sample preparation for SEM and TEM, as well as instrument maintenance. Ability to carry out routine SEM and TEM imaging and x-ray analysis is also desired. For more information on The McCrone Group, please visit www.mccrone.com.
The ideal candidate will have a B.S. degree with coursework in electron microscopy and microtomy, or comparable experience. Compensation is commensurate with education, experience and responsibilities.
Applicant selected must be a U.S. Citizen, will be subject to a government security investigation, and must meet eligibility requirements for access to classified information. McCrone is an Equal Opportunity Employer.
If you have microtomy experience and are looking for an interesting and unique career opportunity in materials analysis, send your resume with a letter describing your experience and interests to:
Careers McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, Illinois 60559
FAX: 630-887-7417 - Attn: Human Resources E-mail: careers-at-mccrone.com (e-mail is preferred)
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Fri Jan 13 12:14:59 2006 11, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 11, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DIEwln001747 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jan 2006 12:14:59 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id DA9E61A800B 11, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 13 Jan 2006 12:14:58 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Fri, 13 Jan 2006 12:14:58 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 11, 27 -- Subject: Open Position: Electron Microscopy Technician 11, 27 -- Date: Fri, 13 Jan 2006 12:14:48 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C3103-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Open Position: Electron Microscopy Technician 11, 27 -- Thread-Index: AcYYbT0QYMn4lwyUQBa7kWOedjOuMA== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DIEwln001747 ==============================End of - Headers==============================
What you are seeing is the projected image of the hot filament (light). This means that although you have a hole down the column the electron gun is out of alignment. May I suggest the following.
At no more than 60kV switch off all the lenses and look for a very bright electron beam by adjusting the gun alignment controls. Once you find the beam switch each lens on one at a time, stepping back if the beam disappears.
Good luck
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7802 966067 Web www.emcourses.com
----- Original Message ----- X-from: {aetmicro-at-optonline.net} To: {protrain-at-emcourses.com} Sent: Friday, January 13, 2006 2:22 AM
Group,
Our University in an effort of conservation of energy is making changes as to the HVAC conditions they can provide us. Requests as to equipment shuts downs during non use hours, shuting off computers every time not in use, and increasing lab temperatures beyound 78F are being asked for. Looking for documented case studies pro or con and anyones experience they would be willing to share offline on this issue. Would also like to hear from vendors as to desired environments for SEM and related equipment as well as stored chemicals.
Thanks
Roy Beavers
Southern Methodist University Department of Geological Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 7, 20 -- From rbeavers-at-mail.smu.edu Fri Jan 13 15:12:52 2006 7, 20 -- Received: from s31xe5.systems.smu.edu (s31xe5.systems.smu.edu [129.119.70.74]) 7, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0DLCpEo022529 7, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 13 Jan 2006 15:12:51 -0600 7, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 7, 20 -- Content-class: urn:content-classes:message 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; 7, 20 -- charset="iso-8859-1" 7, 20 -- Subject: Lab Facilities Temperature 7, 20 -- Date: Fri, 13 Jan 2006 15:12:51 -0600 7, 20 -- Message-ID: {3A9F6E299461A44483961A57175598A703402D0F-at-s31xe5.systems.smu.edu} 7, 20 -- X-MS-Has-Attach: 7, 20 -- X-MS-TNEF-Correlator: 7, 20 -- Thread-Topic: Lab Facilities Temperature 7, 20 -- Thread-Index: AcYYhhysHdUwLzATSNSFiQlctfoZlA== 7, 20 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 7, 20 -- To: "Microscopy Listserver" {Microscopy-at-microscopy.com} 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0DLCpEo022529 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (mswift-at-bunham.org) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, January 13, 2006 at 18:18:43 ---------------------------------------------------------------------------
Email: mswift-at-bunham.org Name: Mark Swift
Organization: Burnham Institute
Education: Graduate College
Location: San Diego, CA
Question: We have a Tecnai 12 from FEI, and we would like to know: How do we ensure that in Low Dose our Focus Substates 1 and 2 are on the tilt axis when Alpha angle is not 0 degrees?
I am seeking advice on obtaining a low cost polisher for SEM sample preparation. The samples will be coated thin metal cross-sections embedded in epoxy. The cross-sections will be analyzed by SEM to determine coating thickness. I have a diamond wet saw but I need a polisher but have very little funds available. I found a used Buehler Minimet polisher in my price range ~$1K. I am not familiar with this unit. I have used bigger units like the Buehler Powerpro and the Leco SS-2000. Is the difference mainly a matter of speed and sample size? I plan on doing only about one sample a week. Any advice will be appreciated. Thanks,
Scott LeMaster PPG Industries Inc. lemaster-at-ppg.com
==============================Original Headers============================== 4, 23 -- From lemaster-at-ppg.com Mon Jan 16 09:31:25 2006 4, 23 -- Received: from SGOFSMTP02.nac.ppg.com (smtpout.ppg.com [141.189.251.4]) 4, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GFVMxY018188 4, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 09:31:24 -0600 4, 23 -- Received: from SGOFUSR24.nac.ppg.com ([10.49.120.133]) by SGOFSMTP02.nac.ppg.com with Microsoft SMTPSVC(5.0.2195.6713); 4, 23 -- Mon, 16 Jan 2006 10:31:20 -0500 4, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.0.6603.0 4, 23 -- Content-Class: urn:content-classes:message 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: text/plain; 4, 23 -- charset="us-ascii" 4, 23 -- Subject: SEM advice on low cost polisher 4, 23 -- Date: Mon, 16 Jan 2006 10:31:20 -0500 4, 23 -- Message-ID: {B92A132E126C4E45B3AAC00B5623D3E7015AF8CA-at-sgofusr24.nac.ppg.com} 4, 23 -- X-MS-Has-Attach: 4, 23 -- X-MS-TNEF-Correlator: 4, 23 -- Thread-Topic: SEM advice on low cost polisher 4, 23 -- thread-index: AcYaseX2g8/b/IiKQ069aQDRzXKGcA== 4, 23 -- From: "LeMaster, J. Scott" {lemaster-at-ppg.com} 4, 23 -- To: {Microscopy-at-microscopy.com} 4, 23 -- X-OriginalArrivalTime: 16 Jan 2006 15:31:20.0666 (UTC) FILETIME=[E69473A0:01C61AB1] 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0GFVMxY018188 ==============================End of - Headers==============================
Scott: on the MiniMet, the sample moves and the polishing media stays still. This tool can take up to a 2 inch potted sample and uses 4-inch PSA grinding/polishing media on glass discs. It was designed to do rough and fine polish and it does that pretty well. On this tool, you drill a small blind hole in the top of your sample. This is where a spring-loaded arm fits to move the sample in an random orbital motion. You have some control over the pressure of the sample into the media and the speed of rotation. It can run unattended. This tool might be just what you need for one sample a week, if you can cut almost to the area of interest and then polish the rest of the way. On used tools, the spring force is usually the first thing to go due to rust, so be aware.
lemaster-at-ppg.com wrote:
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-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 5, 23 -- From r-holdford-at-ti.com Mon Jan 16 10:43:49 2006 5, 23 -- Received: from reloaded.ext.ti.com (reloaded.ext.ti.com [192.94.94.6]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GGhmBh028324 5, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jan 2006 10:43:48 -0600 5, 23 -- Received: from dlep30.itg.ti.com ([157.170.170.32]) 5, 23 -- by reloaded.ext.ti.com (8.13.4/8.13.4) with ESMTP id k0GGh3mi021873; 5, 23 -- Mon, 16 Jan 2006 10:43:32 -0600 (CST) 5, 23 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 5, 23 -- by dlep30.itg.ti.com (8.12.11/8.12.11) with ESMTP id k0GGf6pY012416; 5, 23 -- Mon, 16 Jan 2006 10:41:07 -0600 (CST) 5, 23 -- Message-ID: {43CBCCA2.2060902-at-ti.com} 5, 23 -- Date: Mon, 16 Jan 2006 10:41:06 -0600 5, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 5, 23 -- Organization: SC Packaging Development -- FA Development 5, 23 -- User-Agent: Mozilla Thunderbird 0.8 (Windows/20040913) 5, 23 -- X-Accept-Language: en-us, en 5, 23 -- MIME-Version: 1.0 5, 23 -- To: lemaster-at-ppg.com, MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 23 -- Subject: Re: [Microscopy] SEM advice on low cost polisher 5, 23 -- References: {200601161533.k0GFXXfg018559-at-ns.microscopy.com} 5, 23 -- In-Reply-To: {200601161533.k0GFXXfg018559-at-ns.microscopy.com} 5, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The College of Microscopy will be offering a short course, Scanning Electron Microscopy, March 27-31, 2006, at our Westmont Facility. In addition to lectures, the course emphasizes hands-on training using five scanning electron microscopes and electron microprobe analyzers, and gives students the opportunity to work on their own samples. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 8, 27 -- From eschumacher-at-mccrone.com Mon Jan 16 11:56:32 2006 8, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122]) 8, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GHuW0p005961 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jan 2006 11:56:32 -0600 8, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 8, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 797C11A800B 8, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 16 Jan 2006 11:56:34 -0600 (CST) 8, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 8, 27 -- by pgp.mccrone.com (PGP Universal service); 8, 27 -- Mon, 16 Jan 2006 11:56:34 -0600 8, 27 -- X-PGP-Universal: processed 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="US-ASCII" 8, 27 -- Content-class: urn:content-classes:message 8, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 8, 27 -- Subject: Short Course Announcement: Scanning Electron Microscopy 8, 27 -- Date: Mon, 16 Jan 2006 11:56:21 -0600 8, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A74C310A-at-MCCRONEMSG.tmg.mccrone.com} 8, 27 -- X-MS-Has-Attach: 8, 27 -- X-MS-TNEF-Correlator: 8, 27 -- Thread-Topic: Short Course Announcement: Scanning Electron Microscopy 8, 27 -- thread-index: AcYaxijJDPWYg/gFR2u07q3UCQ75Vw== 8, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 8, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0GHuW0p005961 ==============================End of - Headers==============================
DISCLAIMER: South Bay Technology produces equipment and supplies as described below and, therefore, has a vested interest in promoting their use.
If you are mounting the sample in AcryliMet cold mount or something similar and can hold your sample by hand, then something like our Model 900 Grinder/Polisher would be ideal. A new Model 900 falls into about the same price range as the used Minimet you referred to. This system is often used for low volume polishing requirements - although usually for higher volume than just 1 sample per week. If you have unencapsulated samples or simply want more control over the polishing process, you can use the Model 900 together with something like our Tripod Polisher® Cross Section Polisher, our BiPod Cross Section Polisher or one of our other lapping and polishing fixtures. You can find information on the Model 900 Polisher at http://southbaytech.com/products_index.cfm?main_action=product_detail&ProductID=42.
If you do end up with the Minimet Polisher, I do have a large lot of Minimet Consumables available at a huge discount. If you have an interest in those, please let me know and I will send you the listing.
Best regards-
David
lemaster-at-ppg.com wrote:
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==============================Original Headers============================== 20, 21 -- From henriks-at-southbaytech.com Mon Jan 16 13:06:35 2006 20, 21 -- Received: from ylpvm25.prodigy.net (ylpvm25-ext.prodigy.net [207.115.57.56]) 20, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GJ6ZDe016034 20, 21 -- for {microscopy-at-msa.microscopy.com} ; Mon, 16 Jan 2006 13:06:35 -0600 20, 21 -- Received: from southbaytech.com (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 20, 21 -- (authenticated bits=0) 20, 21 -- by ylpvm25.prodigy.net (smtpauth/dk/flock 8.13.4/8.13.4) with ESMTP id k0GJ68Ik027256; 20, 21 -- Mon, 16 Jan 2006 14:06:10 -0500 20, 21 -- Message-ID: {43CBEEC8.3020505-at-southbaytech.com} 20, 21 -- Date: Mon, 16 Jan 2006 11:06:48 -0800 20, 21 -- From: David Henriks {henriks-at-southbaytech.com} 20, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 20, 21 -- X-Accept-Language: en-us, en 20, 21 -- MIME-Version: 1.0 20, 21 -- To: lemaster-at-ppg.com 20, 21 -- CC: Microscopy Listerver {microscopy-at-msa.microscopy.com} 20, 21 -- Subject: Re: [Microscopy] SEM advice on low cost polisher 20, 21 -- References: {200601161548.k0GFmtuG021248-at-ns.microscopy.com} 20, 21 -- In-Reply-To: {200601161548.k0GFmtuG021248-at-ns.microscopy.com} 20, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20, 21 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I just returned from a trip out of the country, and found an inquiry about starting up ion pumps in among 1200 other assorted e-mail messages (mostly spam), and so I apologize for being so delayed in answering.
I don't know the exact details of the arrangement of the vacuum system on a JEOL 2010, but in general there should be no requirement for having cooling water running while starting up or running a sputter ion pump. In fact, one of the principle advantages of these pumps is the fact that they do not need cooling of any sort for normal operation.
What you would need to do is to set the valves in the vacuum system so that the backing pump evacuates the region of the vacuum system served by the ion pumps, then evacuate this region to a vacuum in the low 10-3 torr range. Then, follow the start-up procedure recommended for your ion pumps. You want to minimize the time the backing pumps are operated in the low end of this pressure range to reduce the possibility of contaminating the system by back streaming of oil from them, otherwise, there should be no problem starting up the ion pumps. As soon as they do start you want to close whatever valves are available to isolate the part of the system they serve from the part evacuated by the other pumps. Once the supply of cooling water is restored, use the diffusion pump to evacuate the rest of the system to an operating level, and then open the valves to the ion-pumped region and resume normal operation.
If you can get hold of a copy of my book on Vacuum Methods in Electron Microscopy (ISBN 1 85578 053 4) you can find the matter of backstreaming from rotary vane pumps discussed in Section 4.1.5 on p. 144. The general operating characteristics of sputter ion pumps is discussed in Sections 7.1.6, p 290 and 7.5, p. 322. I believe the operating procedure for the vacuum system described in Section 9.3 on p. 392 is very similar to that for the 2010.
Good luck, Wil Bigelow
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-662-5237 Address mail to: 1136 Mixtwood Rd. Ann Arbor, MI 48103-3035
==============================Original Headers============================== 7, 14 -- From bigelow-at-engin.umich.edu Mon Jan 16 14:34:37 2006 7, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 7, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0GKYZh8026672 7, 14 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 14:34:36 -0600 7, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 7, 14 -- by srvr22.engin.umich.edu (8.12.10/8.12.10) with ESMTP id k0GKYYGU013312 7, 14 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 15:34:34 -0500 (EST) 7, 14 -- Mime-Version: 1.0 7, 14 -- Message-Id: {p06210201bff1b09494cf-at-[141.212.131.221]} 7, 14 -- Date: Mon, 16 Jan 2006 15:34:33 -0500 7, 14 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 7, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 7, 14 -- Subject: RE:[Microscopy] Starting Ion Pumps without cooling water 7, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
I too am not aware of any water cooling for ion pumps. when first pumping, they do get warm. But their controller will shut them off for a cool down.
The main problem I have had in the past is getting them to start pumping. Initially, no pump current. This is fixed by heating the pump with a hair dryer. Then they fire and that is that. This is typical of the little 1-5L/m gun chamber pumps for LaB6. The larger 30L/m pumps don't seem to have this problem. This was with Varian pumps.
gary g.
At 12:53 PM 1/16/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Mon Jan 16 15:40:09 2006 11, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0GLe9od004433 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 15:40:09 -0600 11, 20 -- Received: (qmail 5325 invoked from network); 16 Jan 2006 13:40:08 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 5322, pid: 5323, t: 0.1787s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp2 with SMTP; 16 Jan 2006 13:40:08 -0800 11, 20 -- Message-Id: {6.2.3.4.2.20060116133653.0206f088-at-mail.calweb.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 20 -- Date: Mon, 16 Jan 2006 13:40:08 -0800 11, 20 -- To: bigelow-at-engin.umich.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps without cooling water 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- References: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I have a multi-function gauge on my T300 that reads from 0-1. It is used with a rotating switch to measure all the voltages etc in the system. When its at full vacuum it reads 0.42 and the manual says it should be 6v which I assume is 0.6. I was wondering if anyone out there knows what this translates to in terms of Torr? Please reply off list if this is not of general interest.
Thanks in advance!
Tom Kaye
==============================Original Headers============================== 5, 20 -- From tom-at-tomkaye.com Mon Jan 16 20:44:11 2006 5, 20 -- Received: from tomkaye.com (mxgateway.techpro.com [64.4.207.8]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0H2iAoG017269 5, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 20:44:10 -0600 5, 20 -- Received: from tkdell [67.165.188.91] by tomkaye.com with ESMTP 5, 20 -- (SMTPD32-8.14) id A9F8638023E; Mon, 16 Jan 2006 20:44:08 -0600 5, 20 -- From: "Tom" {tom-at-tomkaye.com} 5, 20 -- To: {Microscopy-at-microscopy.com} 5, 20 -- Subject: Question about vacuum gauge on JEOL T300 5, 20 -- Date: Mon, 16 Jan 2006 20:44:09 -0600 5, 20 -- Message-ID: {021901c61b0f$e4b081b0$030a1aac-at-tkdell} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- X-Priority: 3 (Normal) 5, 20 -- X-MSMail-Priority: Normal 5, 20 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2910.0) 5, 20 -- Importance: Normal 5, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
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Email: cumings-at-umd.edu Name: John Cumings
Organization: University of Maryland
Title-Subject: [Filtered] Job Opening
Question: Dear Microscopy Community,
I would like to bring your attention to an open position in electron microscopy as the lab manager of a newly-created facility here at the University of Maryland. The position is part of the growing nanoscience center on campus, the Maryland Center for Integrated Nano Science and Engineering (M-CINSE).
More information can be found at http://mse.umd.edu/dept/positions/LabManagerUniversityMicroscopyFacility.htm
and applications can be submitted at https://apra.umd.edu/search.jsp?ID=ENMA000003
Please note that the "best consideration" deadline of Jan. 15th has just passed, so please apply soon if you are interested.
Best regards,
-John Cumings
-- John Cumings cumings-at-umd.edu Assistant Professor Department of Materials Science and Engineering University of Maryland College Park, MD 20742-2115
office (301) 405-0789 (1246 Kim Building) fax (301) 314-8164 --
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Email: nrvrctr-at-adelphia.net Name: Ed Beckett
Organization: MRI Virginia
Title-Subject: [Filtered] SEM
Question: I am searching for a Test engineer in Chicago with experience in SEM as well as other electrical and test abilities. Salary is about $60K. Contact me if you are aware of anyone or self interested. Thanks Ed Beckett 540-980-3100
I am interested to study magnetic structure (type II magnetic contrast) by SEM. Our SEM equipped with a Four Quadrant Backscattered Electron Detector. I will appreciate if you have experience using this type of detector for magnetic domain structure to guide us with the recommended working parameters as combinations of quadrant setting, working distance, tilt and etc.
Thank you in advanced,
Yossi.
==============================Original Headers============================== 5, 30 -- From yezer-at-cc.hut.fi Tue Jan 17 02:22:24 2006 5, 30 -- Received: from smtp-3.hut.fi (smtp-3.hut.fi [130.233.228.93]) 5, 30 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0H8MN4R016682 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jan 2006 02:22:24 -0600 5, 30 -- Received: from localhost (putosiko.hut.fi [130.233.228.114]) 5, 30 -- by smtp-3.hut.fi (8.12.10/8.12.10) with ESMTP id k0H8MM6h013963 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jan 2006 10:22:22 +0200 5, 30 -- Received: from smtp-3.hut.fi ([130.233.228.93]) 5, 30 -- by localhost (putosiko.hut.fi [130.233.228.114]) (amavisd-new, port 10024) 5, 30 -- with LMTP id 22195-17-9 for {Microscopy-at-Microscopy.Com} ; 5, 30 -- Tue, 17 Jan 2006 10:22:22 +0200 (EET) 5, 30 -- Received: from baresi.hut.fi (baresi-m.hut.fi [130.233.228.121]) 5, 30 -- by smtp-3.hut.fi (8.12.10/8.12.10) with ESMTP id k0H8LuRu013837 5, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 17 Jan 2006 10:21:56 +0200 5, 30 -- Received: (from apache-at-localhost) 5, 30 -- by baresi.hut.fi (8.12.10/8.12.6/Submit) id k0H8LtkJ029142 5, 30 -- for Microscopy-at-Microscopy.Com; Tue, 17 Jan 2006 10:21:55 +0200 5, 30 -- To: Microscopy-at-Microscopy.Com 5, 30 -- Subject: Magnetic contrast with 4QBSED 5, 30 -- Message-ID: {1137486115.43cca9239de7c-at-webmail1.hut.fi} 5, 30 -- Date: Tue, 17 Jan 2006 10:21:55 +0200 (EET) 5, 30 -- From: yezer-at-cc.hut.fi 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- User-Agent: HUT webmail, IMP 2.2.6 5, 30 -- X-Authenticated-Sender: yezer-at-cc.hut.fi 5, 30 -- X-Originating-IP: 130.233.108.169 5, 30 -- X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on putosiko.hut.fi 5, 30 -- X-TKK-Virus-Scanned: by amavisd-new-2.1.2-hutcc at putosiko.hut.fi ==============================End of - Headers==============================
The Naval Research Laboratory has a current opening for a Postdoctoral Fellow in the Joining and Transformations Section. The ideal candidate would have a strong background in both electron microscopy and materials science. The successful candidate will study the transformations that occur during joining processes and relate the microstructural features to observed mechanical properties. Current research interests center on the precipitation, dynamic recrystallization, and texture evolution that occur during the friction stir welding process.
The Naval Research Laboratory has an excellent array of microscopy facilities available. There are three transmission electron microscopes (a Phillips CM-30 analytical TEM, a Hitachi H-9000 high-resolution TEM, and a new, state-of-the-art JEOL 2200 energy filtered STEM/TEM), two scanning electron microscopes (a Leo 1550 SEM with electron backscattered diffraction analysis and energy dispersive spectrometry and a Hitachi FEG-SEM), and a dual-beam focused ion beam with EBSD that are available for use by the successful candidate. Additional facilities that may be used include a quenching and deformation dilatometer, a Gleeble thermomechanical simulator, x-ray diffractometers, and an automated microhardness tester.
Please note that this position is only open to US citizens or Green Card holders.
Please contact me by e-mail (Fonda-at-anvil.nrl.navy.mil) or phone (202-767-2622) for further details about this research program.
Sinceerely,
Richard Fonda -- ________________________________________________________________ Richard W. Fonda Naval Research Laboratory (202) 767-2622 Code 6324 (202) 767-2623 fax Washington DC 20375 ________________________________________________________________
==============================Original Headers============================== 6, 14 -- From fonda-at-anvil.nrl.navy.mil Tue Jan 17 06:21:09 2006 6, 14 -- Received: from anvil.nrl.navy.mil (anvil.nrl.navy.mil [132.250.127.188]) 6, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HCL8kO029509 6, 14 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 06:21:09 -0600 6, 14 -- Received: from [132.250.127.172] (rw-fonda.nrl.navy.mil [132.250.127.172]) 6, 14 -- by anvil.nrl.navy.mil (8.12.11/8.12.11) with ESMTP id k0HCL5eT022874 6, 14 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 07:21:06 -0500 (EST) 6, 14 -- Mime-Version: 1.0 6, 14 -- Message-Id: {p06230901bff2920cbcd0-at-[132.250.127.172]} 6, 14 -- Date: Tue, 17 Jan 2006 07:22:41 -0500 6, 14 -- To: Microscopy-at-microscopy.com 6, 14 -- From: "Richard W. Fonda" {fonda-at-anvil.nrl.navy.mil} 6, 14 -- Subject: Postdoctoral Fellowship opening at NRL 6, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: lleroux-at-csir.co.za Name: Lukas le Roux
Organization: CSIR
Title-Subject: [Filtered] Edwards Sputter Coater
Question: I am trying to find an instruction manual for a model S150B Sputter Coater. Is there a website from where I can download it?
Is there a company that can provide room survey services in the Dallas, Texas area? Environmental testing for mechanical vibration and stray EMF parameters are desired.
Please respond directly off list.
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 6, 23 -- From emlabservices-at-cox.net Tue Jan 17 07:35:50 2006 6, 23 -- Received: from centrmmtao06.cox.net (centrmmtao06.cox.net [70.168.83.78]) 6, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HDZm75007409 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 07:35:49 -0600 6, 23 -- Received: from EMLabServices ([24.255.213.54]) by centrmmtao06.cox.net 6, 23 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP 6, 23 -- id {20060117133551.RBDG4002.centrmmtao06.cox.net-at-EMLabServices} 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 08:35:51 -0500 6, 23 -- Message-ID: {008601c61b6a$f0ad2440$6400a8c0-at-EMLabServices} 6, 23 -- From: "EM Lab Services" {emlabservices-at-cox.net} 6, 23 -- To: {Microscopy-at-microscopy.com} 6, 23 -- Subject: TEM Room Survey Company 6, 23 -- Date: Tue, 17 Jan 2006 06:35:53 -0700 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Type: text/plain; 6, 23 -- format=flowed; 6, 23 -- charset="iso-8859-1"; 6, 23 -- reply-type=original 6, 23 -- Content-Transfer-Encoding: 7bit 6, 23 -- X-Priority: 3 6, 23 -- X-MSMail-Priority: Normal 6, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
"I am discussing proposals to install an imaging system here. It is one that can capture both FISH and brown/ H&E stains and then allow an operator, using selected software, to analyse them. The favoured system at present is the Applied Imaging one as it is now undergoing a major facelift.
I am just wondering if there are any other systems on the market right now that can load up 50 slides automatically overnight, scan & store so that an operator can then analyse the images captured the following morning. None of the other major suppliers here in the UK, such as ChromaVision, Meta-systems & Aperio can offer both overnight loading and scanning of both FISH and brown/ blue & pink stains at present."
Gareth Morgan MPhil MSc FIBMS, Department of Histo/cytopathology, Laboratory Medicine (Labmed), Karolinska Institute, Karolinska University Hospital at Huddinge, F46 SE 141 86 Stockholm Sweden
OBS! Besöksadress: F-Huset, Forskningsgatan 2 F52, Rum 2.10. Laboratoriet för klinisk patologi och cytologi.
NB! Visiting address: Building F, Research Corridor 2 F52, Room 2.10. Clinical Histo- and Cytopathology Laboratory.
==============================Original Headers============================== 14, 18 -- From Gareth.Morgan-at-ki.se Tue Jan 17 10:10:41 2006 14, 18 -- Received: from humle.it.ki.se (humle.it.ki.se [130.237.101.252]) 14, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HGAeFM027877 14, 18 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 10:10:41 -0600 14, 18 -- Received: from lgtestdator.ki.se (hsg01.hs.se [193.10.76.5]) 14, 18 -- by humle.it.ki.se (8.13.1/8.13.1) with ESMTP id k0HGAeo4003655 14, 18 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 17:10:40 +0100 (MET) 14, 18 -- Message-Id: {7.0.0.16.0.20060117171215.020160a8-at-ki.se} 14, 18 -- Message-Id: {7.0.0.16.0.20060116170224.020ac968-at-ki.se} 14, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.0.16 14, 18 -- Date: Tue, 17 Jan 2006 17:12:17 +0100 14, 18 -- To: microscopy-at-microscopy.com 14, 18 -- From: Gareth Morgan {Gareth.Morgan-at-ki.se} 14, 18 -- Subject: Image analysis 14, 18 -- Mime-Version: 1.0 14, 18 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 14, 18 -- Content-Transfer-Encoding: 8bit 14, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0HGAeFM027877 ==============================End of - Headers==============================
We're having trouble with bright spots appearing on our tissue when viewing our grids in our new TEM. These spots may be described as bleaching, etching, burning or clearing. The bright spots appears after using high magnification to view an area or to focus. When we go back to a low magnification, we see the bright area.
This started happening with the installation of a new TEM. We haven't changed processing procedures and we are experienced instrument operators. The service engineer has determined that the TEM is working properly. The problem is not due to prolonged exposure to the electron beam. The spots occur within seconds. The filament is positioned correctly. Often there is variability in the intensity of the spotting even on the same grid. In other words, sometime the spot is very apparent and distinctive and other times it's more diffuse. We've tried using liquid nitrogen to improve the vacuum and adding a second 100% resin infiltration step to ensure removal of water from the specimen. The problem occurs with cells and tissues embedded in epoxy. The vendor has suggested working at 120kV and we're starting to do that now. It doesn't entirely correct the problem, but the spots are less focal.
Has anyone worked with a particular epoxy that stands up well or better than others under the electron beam? You can reply off list. Has anyone had this problem and been able find a solution?
Thank you in advance for your time
Marcia
Marcia Pitzenberger Pathology Laboratories Merck & Co., Inc. P.O. Box 4, WP45-104 West Point, PA 19486-0004 Tel 215 652-9767 Fax 215 652-7758 marcia_pitzenberger-at-merck.com
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==============================Original Headers============================== 13, 24 -- From marcia_pitzenberger-at-merck.com Tue Jan 17 11:12:37 2006 13, 24 -- Received: from usryim09.merck.com (taz.merck.com [155.91.6.20]) 13, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HHCaxL008406 13, 24 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 11:12:36 -0600 13, 24 -- Received: from 155.91.2.6 by usryim09.merck.com with ESMTP (SMTP Relay); 13, 24 -- Tue, 17 Jan 2006 12:12:27 -0500 13, 24 -- X-Server-Uuid: 078441B8-8B94-4128-83AA-0F28411313D9 13, 24 -- Received: from 54.3.102.166 by USRYTW32.merck.com with ESMTP (Tumbleweed 13, 24 -- Email Firewall SMTP Relay (Email Firewall v6.1.1)); Tue, 17 Jan 2006 13, 24 -- 12:12:16 -0500 13, 24 -- X-Server-Uuid: 05354929-4888-4C60-B5F7-73C306CFF21D 13, 24 -- Received: by usrygw30.merck.com with Internet Mail Service (5.5.2658.27) 13, 24 -- id {C9J1FVY2} ; Tue, 17 Jan 2006 12:12:16 -0500 13, 24 -- Message-ID: {4F1089C79438304CB5A97E982A11AC1F05AF89-at-usctmx1118.merck.com} 13, 24 -- From: "Pitzenberger, Marcia H." {marcia_pitzenberger-at-merck.com} 13, 24 -- To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-msa.microscopy.com} 13, 24 -- Subject: TEM - Electron Beam Clearing 13, 24 -- Date: Tue, 17 Jan 2006 12:12:07 -0500 13, 24 -- MIME-Version: 1.0 13, 24 -- X-Mailer: Internet Mail Service (5.5.2658.27) 13, 24 -- X-WSS-ID: 6FD3FAFA1X466977-01-01 13, 24 -- X-WSS-ID: 6FD3FAF12C419349-01-01 13, 24 -- Content-Type: text/plain 13, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Jan 14, 2006, at 5:35 AM, mswift-at-bunham.org wrote:
} Question: We have a Tecnai 12 from FEI, and we would like to know: How } do we ensure that in Low Dose our Focus Substates 1 and 2 are on the } tilt axis when Alpha angle is not 0 degrees? } Dear Mark, If you set the focus angles for the substates to 0 and 180 degrees, the displacements will be along the tilt axis. You can verify this by burning a small hole in the ice on a cryogrid in each of the focus substates, then going to a lower mag, where the holes are both visible in the exposure state, and tilting the grid. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Jan 17 12:14:06 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HIE5Ii019810 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 12:14:05 -0600 4, 22 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 9B83B109E08 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 10:14:03 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id B68A633C9D 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 10:14:02 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200601141335.k0EDZ0gB029586-at-ns.microscopy.com} 4, 22 -- References: {200601141335.k0EDZ0gB029586-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {b7eeb9658bb9479e350b822656f74027-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] AskAMicroscopist: Tecnai 12 Focus Substates 4, 22 -- Date: Tue, 17 Jan 2006 10:20:58 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
I speak from a position of ignorance of your type of specimen, however I have picked up on 'This started happening with the installation of a new TEM'.
Are you using a much higher beam current at high mags than previously? Modern TEMs have much better condenser optics than the older ranges available in the 70s and 80s with less use of apertures to reduce spot size and better use of lenses. Have you changed from tungsten to LaB6 or FEG?
These changes can result in more electrons getting to the specimen. Can you compare the beam current that you use at high mag on the two TEMs? If you know typical exposure times of the two instruments do you have to spread the beam more on the new instument to get the same exposure time? Try using a smaller spot size or smaller condenser aperture and see if the effect improves.
Good luck, Ron
In message {200601171752.k0HHqH72018071-at-ns.microscopy.com} marcia_pitzenberger-at-merck.com writes: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } TEM User's - } } } We're having trouble with bright spots appearing on our tissue when viewing } our grids in our new TEM. These spots may be described as bleaching, } etching, burning or clearing. The bright spots appears after using high } magnification to view an area or to focus. When we go back to a low } magnification, we see the bright area. } } This started happening with the installation of a new TEM. We haven't } changed processing procedures and we are experienced instrument operators. } The service engineer has determined that the TEM is working properly. The } problem is not due to prolonged exposure to the electron beam. The spots } occur within seconds. The filament is positioned correctly. Often there is } variability in the intensity of the spotting even on the same grid. In } other words, sometime the spot is very apparent and distinctive and other } times it's more diffuse. We've tried using liquid nitrogen to improve the } vacuum and adding a second 100% resin infiltration step to ensure removal of } water from the specimen. The problem occurs with cells and tissues } embedded in epoxy. The vendor has suggested working at 120kV and we're } starting to do that now. It doesn't entirely correct the problem, but the } spots are less focal. } } Has anyone worked with a particular epoxy that stands up well or better than } others under the electron beam? You can reply off list. Has anyone had } this problem and been able find a solution? } } Thank you in advance for your time } } Marcia } } } } Marcia Pitzenberger } Pathology Laboratories } Merck & Co., Inc. } P.O. Box 4, WP45-104 } West Point, PA 19486-0004 } Tel 215 652-9767 } Fax 215 652-7758 } marcia_pitzenberger-at-merck.com } } } } } } ----------------------------------------------------------------------------- - } Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. } ----------------------------------------------------------------------------- - } } ==============================Original Headers============================== } 13, 24 -- From marcia_pitzenberger-at-merck.com Tue Jan 17 11:12:37 2006 } 13, 24 -- Received: from usryim09.merck.com (taz.merck.com [155.91.6.20]) } 13, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HHCaxL008406 } 13, 24 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 11:12:36 -0600 } 13, 24 -- Received: from 155.91.2.6 by usryim09.merck.com with ESMTP (SMTP Relay); } 13, 24 -- Tue, 17 Jan 2006 12:12:27 -0500 } 13, 24 -- X-Server-Uuid: 078441B8-8B94-4128-83AA-0F28411313D9 } 13, 24 -- Received: from 54.3.102.166 by USRYTW32.merck.com with ESMTP (Tumbleweed } 13, 24 -- Email Firewall SMTP Relay (Email Firewall v6.1.1)); Tue, 17 Jan 2006 } 13, 24 -- 12:12:16 -0500 } 13, 24 -- X-Server-Uuid: 05354929-4888-4C60-B5F7-73C306CFF21D } 13, 24 -- Received: by usrygw30.merck.com with Internet Mail Service (5.5.2658.27) } 13, 24 -- id {C9J1FVY2} ; Tue, 17 Jan 2006 12:12:16 -0500 } 13, 24 -- Message-ID: {4F1089C79438304CB5A97E982A11AC1F05AF89-at-usctmx1118.merck.com} } 13, 24 -- From: "Pitzenberger, Marcia H." {marcia_pitzenberger-at-merck.com} } 13, 24 -- To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-msa.microscopy.com} } 13, 24 -- Subject: TEM - Electron Beam Clearing } 13, 24 -- Date: Tue, 17 Jan 2006 12:12:07 -0500 } 13, 24 -- MIME-Version: 1.0 } 13, 24 -- X-Mailer: Internet Mail Service (5.5.2658.27) } 13, 24 -- X-WSS-ID: 6FD3FAFA1X466977-01-01 } 13, 24 -- X-WSS-ID: 6FD3FAF12C419349-01-01 } 13, 24 -- Content-Type: text/plain } 13, 24 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
==============================Original Headers============================== 7, 27 -- From ron.doole-at-materials.ox.ac.uk Tue Jan 17 12:26:00 2006 7, 27 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 7, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HIPxqX022315 7, 27 -- for {microscopy-at-msa.microscopy.com} ; Tue, 17 Jan 2006 12:25:59 -0600 7, 27 -- Received: from smtp1.herald.ox.ac.uk ([163.1.0.247]) 7, 27 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.54) 7, 27 -- id 1EyvWo-0007nk-WD 7, 27 -- for microscopy-at-msa.microscopy.com; Tue, 17 Jan 2006 18:25:59 +0000 7, 27 -- Received: from webmail222.herald.ox.ac.uk ([163.1.0.222]) 7, 27 -- by smtp1.herald.ox.ac.uk with esmtp (Exim 3.36 #1) 7, 27 -- id 1EyvWo-0004Bf-01 7, 27 -- for microscopy-at-msa.microscopy.com; Tue, 17 Jan 2006 18:25:58 +0000 7, 27 -- Received: by webmail222.herald.ox.ac.uk (Postfix, from userid 101) 7, 27 -- id AD78A2A0B8; Tue, 17 Jan 2006 18:25:58 +0000 (GMT) 7, 27 -- Content-Type: text/plain 7, 27 -- Content-Disposition: inline 7, 27 -- Content-Transfer-Encoding: binary 7, 27 -- MIME-Version: 1.0 7, 27 -- X-Mailer: MIME-tools 5.411 (Entity 5.404) 7, 27 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk} 7, 27 -- Date: Tue, 17 Jan 2006 18:25:58 +0000 (GMT) 7, 27 -- In-Reply-To: {200601171752.k0HHqH72018071-at-ns.microscopy.com} 7, 27 -- To: microscopy-at-msa.microscopy.com 7, 27 -- Subject: Re: [Microscopy] TEM - Electron Beam Clearing 7, 27 -- X-Webmail-Originating-Ip: 192.76.27.49 7, 27 -- X-Webmail-Sender: rdoole 7, 27 -- Message-Id: {20060117182558.AD78A2A0B8-at-webmail222.herald.ox.ac.uk} ==============================End of - Headers==============================
Gary; Hitting ion pumps with a hammer also gets them started. The hammer is also very good at knocking loose whiskers that form in the ion pump.
John Mardinly Intel
The opinion of this author does not necessarily represent the opinion of Intel Corporation.
-----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: Monday, January 16, 2006 1:41 PM To: Mardinly, John
I too am not aware of any water cooling for ion pumps. when first pumping, they do get warm. But their controller will shut them off for a cool down.
The main problem I have had in the past is getting them to start pumping. Initially, no pump current. This is fixed by heating the pump with a hair dryer. Then they fire and that is that. This is typical of the little 1-5L/m gun chamber pumps for LaB6. The larger 30L/m pumps don't seem to have this problem. This was with Varian pumps.
gary g.
At 12:53 PM 1/16/2006, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Mon Jan 16 15:40:09 2006 11, 20 -- Received: from qsmtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0GLe9od004433 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2006 15:40:09 -0600 11, 20 -- Received: (qmail 5325 invoked from network); 16 Jan 2006 13:40:08 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 5322, pid: 5323, t: 0.1787s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp2 with SMTP; 16 Jan 2006 13:40:08 -0800 11, 20 -- Message-Id: {6.2.3.4.2.20060116133653.0206f088-at-mail.calweb.com} 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 11, 20 -- Date: Mon, 16 Jan 2006 13:40:08 -0800 11, 20 -- To: bigelow-at-engin.umich.edu 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps without cooling water 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- References: {200601162053.k0GKrXr6030172-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 20, 34 -- From john.mardinly-at-intel.com Tue Jan 17 15:16:13 2006 20, 34 -- Received: from scsfmr003.sc.intel.com (fmr23.intel.com [143.183.121.15]) 20, 34 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HLGCFX009840 20, 34 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 17 Jan 2006 15:16:13 -0600 20, 34 -- Received: from scsfmr101.sc.intel.com (scsfmr101.sc.intel.com [10.3.253.10]) 20, 34 -- by scsfmr003.sc.intel.com (8.12.10/8.12.10/d: major-outer.mc,v 1.1 2004/09/17 17:50:56 root Exp $) with ESMTP id k0HLGCK7006008; 20, 34 -- Tue, 17 Jan 2006 21:16:12 GMT 20, 34 -- Received: from scsmsxvs041.sc.intel.com (scsmsxvs041.sc.intel.com [10.3.90.10]) 20, 34 -- by scsfmr101.sc.intel.com (8.12.10/8.12.10/d: major-inner.mc,v 1.2 2004/09/17 18:05:01 root Exp $) with SMTP id k0HLC3jR001619; 20, 34 -- Tue, 17 Jan 2006 21:12:06 GMT 20, 34 -- Received: from scsmsx331.amr.corp.intel.com ([10.3.90.4]) 20, 34 -- by scsmsxvs041.sc.intel.com (SAVSMTP 3.1.7.47) with SMTP id M2006011713160707553 20, 34 -- ; Tue, 17 Jan 2006 13:16:07 -0800 20, 34 -- Received: from scsmsx403.amr.corp.intel.com ([10.3.90.18]) by scsmsx331.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.211); 20, 34 -- Tue, 17 Jan 2006 13:16:07 -0800 20, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 20, 34 -- Content-class: urn:content-classes:message 20, 34 -- MIME-Version: 1.0 20, 34 -- Content-Type: text/plain; 20, 34 -- charset="us-ascii" 20, 34 -- Subject: RE: [Microscopy] Starting Ion Pumps without cooling water 20, 34 -- Date: Tue, 17 Jan 2006 13:16:03 -0800 20, 34 -- Message-ID: {9E1ED6A623D8CB44B4866ACF20ECDB2B08CC4267-at-scsmsx403.amr.corp.intel.com} 20, 34 -- X-MS-Has-Attach: 20, 34 -- X-MS-TNEF-Correlator: 20, 34 -- Thread-Topic: [Microscopy] Starting Ion Pumps without cooling water 20, 34 -- Thread-Index: AcYa5dJSmizC6M7cSkqL66kBhLViXgAxSKoQ 20, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 20, 34 -- To: {gary-at-gaugler.com} 20, 34 -- Cc: {Microscopy-at-MSA.Microscopy.Com} 20, 34 -- X-OriginalArrivalTime: 17 Jan 2006 21:16:07.0634 (UTC) FILETIME=[3B630320:01C61BAB] 20, 34 -- X-Scanned-By: MIMEDefang 2.52 on 10.3.253.10 20, 34 -- Content-Transfer-Encoding: 8bit 20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0HLGCFX009840 ==============================End of - Headers==============================
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Several glass plates ~5 mm thick and 15 cm square. Polishing clothes stuck to the glass plates. Glass plates inside large tray. Water spray to lubricate. Cost is almost nothing apart from the time. Depends how large the sample is and what finish you are working from. Fine grinding can be done by using aumina directly onto the glass. It's the way I used to prepare 3 mm steel discs for TEM, before a final electropolish. A 3 mm stainless steel disc takes ~15 mins to polish by hand from the alumina grinding.
-- Larry Stoter
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
==============================Original Headers============================== 4, 16 -- From larry-at-cymru.freewire.co.uk Tue Jan 17 15:45:45 2006 4, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250]) 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0HLjhPu015336 4, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 17 Jan 2006 15:45:44 -0600 4, 16 -- Received: from [217.154.248.196] (th1dc-217-154-248-196.dial.mistral.co.uk [217.154.248.196]) 4, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id k0HLjdC13695; 4, 16 -- Tue, 17 Jan 2006 21:45:40 GMT 4, 16 -- Mime-Version: 1.0 4, 16 -- Message-Id: {p06210200bff31079bb79-at-[217.154.248.193]} 4, 16 -- In-Reply-To: {200601161546.k0GFkBfX020754-at-ns.microscopy.com} 4, 16 -- References: {200601161546.k0GFkBfX020754-at-ns.microscopy.com} 4, 16 -- Date: Tue, 17 Jan 2006 21:33:35 +0000 4, 16 -- To: lemaster-at-ppg.com, Microscopy-at-MSA.Microscopy.Com 4, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 4, 16 -- Subject: Re: [Microscopy] SEM advice on low cost polisher 4, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Oh yeah. I forgot to mention that trick too. I usually use the handle end of a screwdriver. This also works for cold cathode sensors too.
gary g.
At 01:33 PM 1/17/2006, you wrote:
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==============================Original Headers============================== 8, 20 -- From gary-at-gaugler.com Tue Jan 17 17:13:09 2006 8, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 8, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0HND8OF000462 8, 20 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2006 17:13:08 -0600 8, 20 -- Received: (qmail 2929 invoked from network); 17 Jan 2006 15:13:06 -0800 8, 20 -- Received: by simscan 1.1.0 ppid: 2926, pid: 2927, t: 0.2584s 8, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 8, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 8, 20 -- by qsmtp1 with SMTP; 17 Jan 2006 15:13:06 -0800 8, 20 -- Message-Id: {6.2.3.4.2.20060117151146.01febf50-at-mail.calweb.com} 8, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 8, 20 -- Date: Tue, 17 Jan 2006 15:13:07 -0800 8, 20 -- To: john.mardinly-at-intel.com 8, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 8, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps without cooling water 8, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 8, 20 -- In-Reply-To: {200601172133.k0HLXwjI013245-at-ns.microscopy.com} 8, 20 -- References: {200601172133.k0HLXwjI013245-at-ns.microscopy.com} 8, 20 -- Mime-Version: 1.0 8, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
With some degree of simplification- low signal electronic imaging is a race between noise accumulation and signal accumulation.
Some of the noise sources in the CCD are:
a) thermal noise (reduced by factor of 2 for every 6 to 7 degrees C temperature drop for silicone CCD), mostly correctable by dark frame subtraction; b) readout (pixel clock switching) noise also know as bias noise, somewhat reduced by cooling, correctable by bias frame subtraction; c) random (shot) noise - a random part of thermal noise (a) which can not be corrected by dark frame subtraction- this noise gets worse with pixels size shrinking (the larger the pixels the less random this value is)- a crude comparison is a mechanical vibration dumping by increasing weight (anti-vibration platform).
Assuming your signal source is set (optics, specimen, illumination):
a) the colder the sensor- the better S/N ratio is- noise accumulation slows down, while signal stays the same; b) with 30 deg. C difference noise will be different by factor of 32 (30deg. = 6deg.*5; noise drops twice per every 6 degrees, thus 2 power 5 = 32 which is a dramatic noise reduction, but noticeable only if light is dim and exposures are long. c) the larger the pixels- the better S/N ratio is- at the expense of spatial resolution- it could pay to have more smaller pixels and use binning instead when needed.
Type of sensor:
a) definitely CCD over CMOS; b) probably full frame CCD over interline CCD, (full frame has much better S/N ratio for a given pixel size), but interline CCD could be more convenient due to potentially faster frame rate, exposure time allowing; c) if exposure time must be long, interline CCD looses it's potential speed advantage, and it is always noisier than full frame one; d) interline CCD has typically 50% of it's light receiving area occupied by transfer gates, meaning that only half of it is light sensitive- this can be improved by micro lens array - most interline CCDs are available in such configuration), but sensitive area of the pixel is still half of what it could be in full frame CCD with same pixel size, which means higher noise .
Interline CCDs can use frame integration with less or no cooling and achieve decent noise reduction, but this is always inferior to deeply cooled full frame CCD in single frame (long exposure) integration mode, with respect to S/N.
I deliberately used S/N (signal to noise) ratio instead just "noise", because it is precisely ratio that counts. If signal is strong (bright light), then it accumulates much faster than noise. Then exposures are short, and no CCD cooling needed. There is no straight answer without knowing the numbers for a given application.
A specific application could be best served by either a CMOS, or a full frame CCD, or interline CCD sensor, cooled or not- many more variables must be taken into account.
Vitaly Feingold SIA 2773 Heath Line Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com
----- Original Message ----- X-from: {phillipst-at-missouri.edu} To: {vitalylazar-at-att.net} Sent: Friday, January 13, 2006 12:54 PM
Overview: Schafer Vallecitos Laboratory is seeking a Senior Engineer /Scientist to add to our mass spectrometry group. SVL performs materials characterization and related analytical services on commercial and government contracts. The activities of the group include chemical and elemental analysis of materials on a production basis, maintenance of several mass spectrometers and ancillary equipment, development of new or improved MS analysis techniques, and quality assurance of analytical data. Schafer is a technically strong firm with a reputation for quality and integrity. Schafer's reputation is a direct result of our dedicated, motivated, talented, and creative staff that is responsible for developing our outstanding business relationships with our customers. Our technical capabilities are vast and growing to provide innovations for the future.
Responsibilities: This position requires an individual who has proven technical abilities to function as an instrument designer, with emphasis on ion optics, electronics, and computer automation. Commercial design experience would be a plus, as the instrument should be maintainable and replicable, not a one-of-a-kind that requires a large staff to maintain. The ideal candidate must have a strong background in materials science or engineering, or a related field of physics, geology, chemistry, or metrology. Experience in design and operation of mass spectrometers, particularly TIMS, and other analytical instruments, is highly recommended. The candidate should have competence in vacuum technology, electronics, mechanical design, ion optics, and project administration.
Qualifications: Experience with analytical instrument control computer hardware and software is required. The candidate must be able to operate independently with occasional supervision.
Other qualifications include:
. Bachelor's degree in physical science or engineering. . Minimum 10 years of technical experience. . Demonstrated ability to solve technical problems. . Expertise in data evaluation and quality control. . Proven ability to write clear scientific reports and proposals. . Must be a US citizen with the ability to obtain government security clearance.
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There are two systems I know of that he may have overlooked:
One is the (dot)Scan, by Soft Imaging Systems The second is the Mirax system, by Carl Zeiss
I know they can physcially do what you require (overnight, automated loading and unloading), but I don't know their abilities in terms of stains.
Hope that helps a little bit! -Chris
--------------------------------- Christopher Hayden SPA Novartis Pharmaceuticals
Hi
Can anyone out there help a colleague?
"I am discussing proposals to install an imaging=20 system here. It is one that can capture both FISH=20 and brown/ H&E stains and then allow an operator,=20 using selected software, to analyse them. The=20 favoured system at present is the Applied Imaging=20 one as it is now undergoing a major facelift.
I am just wondering if there are any other=20 systems on the market right now that can load=20 up 50 slides automatically overnight, scan &=20 store so that an operator can then analyse the=20 images captured the following morning. None of=20 the other major suppliers here in the UK, such as=20 ChromaVision, Meta-systems & Aperio can offer=20 both overnight loading and scanning of both FISH=20 and brown/ blue & pink stains at present."
Dear Yossi, I have not seen a reply to your inquiry, so I will tell you what I know, which isn't much. Of all of the mechanisms for contrast in the back-scattered-electron (BSE) detector, the magnetic domain is the lowest. First is atomic number, second is channeling contrast and third is magnetic domain. This means you must eliminate all others to see the magnetic domain. The sample must be single phase, preferably single crystal and flat, parallel to the BSE detector, well-polished and, perhaps, electropolished. I have not done this, only seen talks about it. It takes a lot of beam current, an optimum working distance for the BSE (about 20 to 15 mm), all quadrants on and contrast on the BSE amplifier as high as practical. And a lot of patience. Good luck. Regards, Mary Mager Electron Microscopist Department of Materials Engineering University of British Columbia 6350 Stores Road Vancouver, B.C. V6T 1Z4 CANADA Tel: 604-822-5648 Fax: 604-822-3619 e-mail: mager-at-interchange.ubc.ca ----- Original Message ----- X-from: {yezer-at-cc.hut.fi} To: {mager-at-interchange.ubc.ca} Sent: Tuesday, January 17, 2006 1:01 AM
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Email: bucana-at-audumla.mdacc.tmc.edu Name: Corazon D. Bucana
Organization: UT MD Anderson Cancer Center
Title-Subject: [Filtered] Separation of resin from glass tissue culture chamber slides
Question: We have emdedded tissue culture cells grown on tissue culture glass chambered slides using Polybed Epon 812, polymerized at 60C for 2 days. We tried to separate the glass by using liquid nitrogen followed by warm water bath but the glass did not separate from the polymerized resin. Can anyone suggest an alternative procedure to remove the glass from the block? Thanks.
We grow the cells on a coverslip (round coverslips are better and they will fit into the chamber) and remove them by hydrofluoric acid afterwards. Works very well, although the HF is not very pleasant to work with.
Good luck,
M.
-- Michael Jarnik, Ph.D. Electron Microscope Facility Fox Chase Cancer Center 7701 Burholme Ave. Philadelphia, PA 19111 Tel. 215-728-5675 Fax 215-728-2412
Name: Corazon D. Bucana
Organization: UT MD Anderson Cancer Center
Title-Subject: Separation of resin from glass tissue culture chamber slides
Question: We have emdedded tissue culture cells grown on tissue culture glass chambered slides using Polybed Epon 812, polymerized at 60C for 2 days. We tried to separate the glass by using liquid nitrogen followed by warm water bath but the glass did not separate from the polymerized resin. Can anyone suggest an alternative procedure to remove the glass from the block? Thanks.
==============================Original Headers============================== 13, 19 -- From M_Jarnik-at-fccc.edu Thu Jan 19 19:23:54 2006 13, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 13, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0K1Nr1r023799 13, 19 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2006 19:23:53 -0600 13, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 13, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k0K1Nrxr008126 13, 19 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2006 20:23:53 -0500 (EST) 13, 19 -- Message-ID: {43D03BA9.5060405-at-fccc.edu} 13, 19 -- Date: Thu, 19 Jan 2006 20:23:53 -0500 13, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 13, 19 -- Reply-To: M_Jarnik-at-fccc.edu 13, 19 -- Organization: Fox Chase Cancer Center 13, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 13, 19 -- X-Accept-Language: en,cs 13, 19 -- MIME-Version: 1.0 13, 19 -- To: microscopy-at-microscopy.com 13, 19 -- Subject: RE: Separation of resin from glass tissue culture chamber slides 13, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 13, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi. We too use concentrated hydrofluoric acid to dissolve glass coverslips. If you use plastic tripour beakers, plastic forceps, and plenty of rinse water, it doesn't cause problems. Since slides are thicker than coverslips, they may need may need longer time in the acid. Coverslips usually dissolve in 20 mins if all the edges are free of resin. To help the acid get at the free edges, file down the edges of the embedded slides using a metal file. Then immerse in acid under the fume hood in a plastic beaker. Check periodically by dipping in water and looking at the surface( wear nitrile gloves and work under the hood) The surface should be smooth and shiny with no patches of undissolved glass( looks like an iceberg melting). Rinse well under running water and dry in the embedding oven. Good luck!
JoAnn Buchanan Stanford University school of Medicine Stanford, CA 94305
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Title-Subject: [Filtered] Immunocytochemistry for TEM
Question: Greetings ListServers,
Does anyone have a general protocol for labelling anti-lucifer yellow using protein-A gold as a bridging antibody in the Locust (Orthopteran) brain? Should I try to immunogold pre-embedding, using a detergent such as Saponin? Should I dissect out the brain before fixation? I am hoping to use the immunogold labelling to help me visualize neuronal connections. Any advice is greatly appreciated.
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Title-Subject: [Filtered] liposomes analyzed by Freeze fracture SEM
Question: Hello,
I work for a company that is interested in getting some liposomes analyzed by Freeze fracture SEM or another SEM type technique. If there is any way you could put me into contact with some of your members who do this type of work or to post my request on a list serve, etc. it would be extremely helpful.
Thank you,
Michael Beverly Sirna Therapeutics, Inc. 303-546-8190
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Title-Subject: [Filtered] NANOBIO summer school in Cargese France
Question: Please find enclosed a flyer on the NANOBIO summer school in Cargese, Corsica, France from July 17th till 29th, 2006.
Watching single biological molecules at work is now possible and the unravelled features of the molecular machinery at the nanoscale are currently changing our view of the cell. Though, bridging the gap between nano- and micro-world is not easy: how to relate single molecule events to biological functions at the cell level? how do molecular structures assemble, coordinate and integrate in the wholeÖ? Thanks to physicists and biologists, we will address these questions and try to give some answers. Speakers will present recent concepts and methods in physics and biology dedicated to single molecule observation and description of the cell at the sub-micron level. Particular emphasis will be given to the optical methods, which allow the observation of live cells in physiological conditions.
This school is part of the Nanosciencestech Marie Curie Series of Events: http://www.france-optique.org/Nanosciencestech.html
The students, including PhDs, post-docs, and young researchers, will be encouraged to present their own work (also in adjacent fields) during poster sessions.
A European and more generally an international audience is targeted, and all lectures in the summer school will be given in English.
Important dates: Pre-registration: Application deadline 28 february 2006 Selected participant will be informed before the end of march
Registration and paying attendees deadline: 30 April 2006
All correspondence should be sent to
SociČtČ FranĮaise d'Optique Nanobio summer school c/o FranĮoise CHAVEL Centre Scientifique d'Orsay - Bt. 503 91403 ORSAY cedex France
next time, you should only polymerize for about 7-8 hours before popping the cells off. I then usually re-embed the pieces that come off and polymerize using standard times.
At 06:43 PM 01/19/06, you wrote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Greetings ListServers, does anybody have any experience using TUNEL assay on plants? I use the kit from Roche, nucleotides dyed with TMR, and have a problem that all the seccions (free-hand,fixed,penetrated with Triton and pectinase/cellulase solution), even those treated just with labeling solution without enzymes, are TUNEL positive. Do you know any reasonable explanation how could the nucleotides bind on the nucleus (and slightly on plasmatic membrane) without enzymes added?
Best regards,
Zuzana Lenochova Charles University Prague
==============================Original Headers============================== 7, 21 -- From lenoska1-at-seznam.cz Fri Jan 20 09:10:21 2006 7, 21 -- Received: from mx1.seznam.cz (mx1.seznam.cz [212.80.76.26]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0KFAIIa017250 7, 21 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 Jan 2006 09:10:19 -0600 7, 21 -- Received: (qmail 21368 invoked by uid 0); 20 Jan 2006 15:10:18 -0000 7, 21 -- To: Microscopy-at-Microscopy.Com 7, 21 -- Date: Fri, 20 Jan 2006 16:10:16 +0100 (CET) 7, 21 -- Reply-To: =?iso-8859-2?Q?Zuzana=20Lenochov=E1?= {lenoska1-at-seznam.cz} 7, 21 -- From: =?iso-8859-2?Q?Zuzana=20Lenochov=E1?= {lenoska1-at-seznam.cz} 7, 21 -- Received: from [195.113.47.73] 7, 21 -- by email.seznam.cz with HTTP 7, 21 -- for lenoska1-at-seznam.cz; 7, 21 -- Fri, 20 Jan 2006 16:02:18 +0100 (CET) 7, 21 -- Subject: =?us-ascii?Q?fluorescence=20microscopy?= 7, 21 -- Mime-Version: 1.0 7, 21 -- Message-Id: {4290.7421-27152-1432019944-1137769816-at-seznam.cz} 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="us-ascii" 7, 21 -- X-Abuse: helpdesk-at-seznam.cz 7, 21 -- X-Seznam-User: lenoska1-at-seznam.cz ==============================End of - Headers==============================
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I've never done this and I'm trying to remember an illustration from an old JEOL SEM brochure, which described an attachment for magnetic domain imaging.
From what I recall, the experimental setup was similar to that used now for EBSD. Sample at a high tilt with a detector to one side. In this case, a BSE detector. The idea was that the weak effect of the magnetic domains caused a small divergence of BS electrons. By positioning the detector a long way from the sample and at a high angle from the incident beam, the divergence was amplified. -- Larry Stoter
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Even though I am very experienced in Electron Microscopy, there are times when I cut LM sections that are extremely wrinkled, and they look like cracked glass. I normally don't experience this, but when it happens, even considering every possible variable, I'm still not exactly sure what might be at work here to give this sort of result.
I'm looking for ideas here as to what might be causing this effect. What do people think?
Garry Burgess Charge Technologist - Electron Microscopy Health Sciences Centre Winnipeg, Canada.
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==============================Original Headers============================== 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Jan 20 16:10:02 2006 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0KMA1mn017769 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2006 16:10:01 -0600 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 6, 20 -- {B0017584293-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Fri, 6, 20 -- 20 Jan 2006 16:09:53 -0600 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 6, 20 -- (5.5.2653.19)id {T7CLC1WX} ; Fri, 20 Jan 2006 16:10:27 -0600 6, 20 -- Message-ID: 6, 20 -- {00A937989100304A83A058F6C45873FF32A36C-at-hscxntmx0005.hsc.mb.ca} 6, 20 -- Date: Fri, 20 Jan 2006 16:06:48 -0600 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6, 20 -- Subject: Wrinkled LM Sections 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
in my experience (I am familiar with large semithin sections = up to 5 x 5 mm from human diagnostic samples for some 20 years now) your question cannot be answered with one sentence. There are several parameters to be included in a thoroughly checking of causes, starting from the type of tissue, via fixation, (full) dehydration, intermedium (full evaporation/complete exchange by resin), type, quality [polymerisation, hardness] of resin, quality of knife edge, sectioning parameters (knife, cutting angle, speed) and type of transfer of sections from the water trough to the slide, and last but not least, "special treatment" of the floating section on a water drop (i.e., for example, trying to spread sections by xylene vapor [other - more healthier - vapors?] or gentle - longer - warming up by means of a light bulb positioned above the section/slide). So IMO, you have to discriminate the problem(s) from the whole processing schedule.......(;-(.....
You tell us that you face problems not everytime, but sometimes..... Could you give us some examples (tissue type, some hints on chemicals /resin you use?) where you get such results? (not to forget the dimensions of the tissue blocks and type of knife used)....
Perhaps there is a simple solution......who knows,
best regards and have a nice weekend,
Wolfgang Muss
Salzburg, Austria
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
Mobile-phone private: ++43+676+5 369-456 E-Mail private: wij.Muss-at-aon.at ------------------------------------------------------------------------ --------------------------------Information on behalf of Society for Cutaneous Ultrastructure Research (SCUR) PLEASE VISIT THE UPDATED WEBSITE of SCUR at } http://www.scur.org { ------------------------------------------------------------------------- Forthcoming Meetings: 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland WEBSITE, containing all FORMS: http://www.scur.org.pl Additional informations: send an E-Mail kwoznia-at-amwaw.edu.pl ---------------------- 34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE Czech Republic ---------------------- 35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan Joint Meeting with the JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology
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I'm looking for ideas here as to what might be causing this effect. What do people think?
Garry Burgess Charge Technologist - Electron Microscopy Health Sciences Centre Winnipeg, Canada.
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==============================Original Headers============================== 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Jan 20 16:10:02 2006 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0KMA1mn017769 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2006 16:10:01 -0600 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 6, 20 -- {B0017584293-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Fri, 6, 20 -- 20 Jan 2006 16:09:53 -0600 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 6, 20 -- (5.5.2653.19)id {T7CLC1WX} ; Fri, 20 Jan 2006 16:10:27 -0600 6, 20 -- Message-ID: 6, 20 -- {00A937989100304A83A058F6C45873FF32A36C-at-hscxntmx0005.hsc.mb.ca} 6, 20 -- Date: Fri, 20 Jan 2006 16:06:48 -0600 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 6, 20 -- Subject: Wrinkled LM Sections 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
==============================Original Headers============================== 25, 28 -- From W.Muss-at-salk.at Sat Jan 21 04:43:58 2006 25, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) 25, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LAhv9E003479 25, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 04:43:57 -0600 25, 28 -- Received: from localhost (localhost [127.0.0.1]) 25, 28 -- by hermes.lks.at (Postfix) with ESMTP id B185A5A9046; 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) 25, 28 -- Received: from hermes.lks.at ([127.0.0.1]) 25, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) 25, 28 -- with ESMTP id 42900-09; Sat, 21 Jan 2006 11:43:51 +0100 (CET) 25, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) 25, 28 -- by hermes.lks.at (Postfix) with SMTP id 754D65A9044; 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) 25, 28 -- Received: by localhost with Microsoft MAPI; Sat, 21 Jan 2006 11:43:49 +0100 25, 28 -- Message-ID: {01C61E7F.F216D3E0.W.Muss-at-salk.at} 25, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} 25, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} 25, 28 -- To: "'GBurgess-at-exchange.hsc.mb.ca'" {GBurgess-at-exchange.hsc.mb.ca} , 25, 28 -- "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 25, 28 -- Subject: [Microscopy] Re: Wrinkled LM Sections 25, 28 -- Date: Sat, 21 Jan 2006 11:43:48 +0100 25, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} 25, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor 25, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 25, 28 -- MIME-Version: 1.0 25, 28 -- Content-Type: text/plain; charset="us-ascii" 25, 28 -- Content-Transfer-Encoding: 7bit 25, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at ==============================End of - Headers==============================
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Email: wong-at-msg.ucsf.edu Name: Mei Lie Wong
Title-Subject: [Filtered] formvar
Question: When using formvar in ethylene dichloride, is the any way to make the films slightly thicker. I know when using formvar in chloroform, you can just drain slower or faster to make films thinner or thicker. Is there something like this for the ethylene dichloride formula? I have tried several different times but so far haven't hit on anything totally satisfactory.
Mei Lie Wong wrote: ======================================================== Question: When using formvar in ethylene dichloride, is the any way to make the films slightly thicker. I know when using formvar in chloroform, you can just drain slower or faster to make films thinner or thicker. Is there something like this for the ethylene dichloride formula? I have tried several different times but so far haven't hit on anything totally satisfactory. ======================================================= This is discussed on http://www.2spi.com/catalog/submat/sup_film6.html
Disclaimer: SPI Supplies manufactures Formvar coated grids for customers and we disclose some of the tricks we use for making thicker or thinner films when using ethylene dichloride.
Chuck
============================================
Charles A. Garber, Ph. D. Ph: 1-610-436-5400 President SPI SUPPLIES FAX: 1-610-436-5755 PO BOX 656 e-mail:cgarber-at-2spi.com West Chester, PA 19381-0656 USA Cust.Service: spi2spi-at-2spi.com
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==============================Original Headers============================== 10, 25 -- From cgarber-at-2spi.com Sat Jan 21 10:41:36 2006 10, 25 -- Received: from diskless5.axs2000.net (mail.axs2000.net [209.120.196.43]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LGfaP8025961 10, 25 -- for {microscopy-at-msa.microscopy.com} ; Sat, 21 Jan 2006 10:41:36 -0600 10, 25 -- Received: from ibm1x23g2abfyg ([71.225.72.4]) 10, 25 -- by diskless5.axs2000.net (8.12.11/8.12.11) with SMTP id k0LGfYOZ012677; 10, 25 -- Sat, 21 Jan 2006 11:41:35 -0500 10, 25 -- X-IDV-FirstRcvd: [71.225.72.4] 10, 25 -- X-IDV-HELO: ibm1x23g2abfyg 10, 25 -- Message-ID: {01e401c61ea9$883ec840$6401a8c0-at-ibm1x23g2abfyg} 10, 25 -- Reply-To: "Garber, Charles A." {cgarber-at-2spi.com} 10, 25 -- From: "Garber, Charles A." {cgarber-at-2spi.com} 10, 25 -- To: "Microscopy Listserver" {microscopy-at-msa.microscopy.com} 10, 25 -- Subject: Formvar filmed grids: controlling thickness 10, 25 -- Date: Sat, 21 Jan 2006 11:41:30 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Type: text/plain; 10, 25 -- format=flowed; 10, 25 -- charset="Windows-1252"; 10, 25 -- reply-type=original 10, 25 -- Content-Transfer-Encoding: 7bit 10, 25 -- X-Priority: 3 10, 25 -- X-MSMail-Priority: Normal 10, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 10, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
I was interested in the comments about banging on the sides of a sputter ion pump with a hammer or screw driver to overcome the startup problem that arises when a whisker or flake of titanium has formed and shorted out the path between the cathode and anode of the pump. Another way to treat this problem is discussed on p. 295 of my book 'Vacuum Methods in Electron Microscopy' (available from SPI, Ladd, M.E. Taylor, etc. For a description see: www.2spi.com/catalog/books/book48.html). This involves allowing the pressure in the pump to rise slightly above 1 Pa (10-2 Torr) and then turning on the high voltage power for a few seconds for three or four times. Be careful not to do this long enough to damage the power supply. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-engin.umich.edu; Fx:734-763-4788; Ph:734-662-5237 Address mail to: 1136 Mixtwood Rd. Ann Arbor, MI 48103-3035
==============================Original Headers============================== 1, 14 -- From bigelow-at-engin.umich.edu Sat Jan 21 11:29:43 2006 1, 14 -- Received: from srvr22.engin.umich.edu (srvr22.engin.umich.edu [141.213.75.21]) 1, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LHTgo2002820 1, 14 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 11:29:42 -0600 1, 14 -- Received: from [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- by srvr22.engin.umich.edu (8.13.5/8.13.5) with ESMTP id k0LHTfRg008116 1, 14 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 12:29:42 -0500 (EST) 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06210202bff81ae0e465-at-[141.212.131.221]} 1, 14 -- Date: Sat, 21 Jan 2006 12:29:41 -0500 1, 14 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-engin.umich.edu} 1, 14 -- Subject: [Microscopy] RE: Starting Ion Pumps 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Yes, that might also work. However, if I just got through with an 8 hour bakeout, why would I want to degrade vacuum in the gun chamber? I would either never get the chamber pumped down or it would take a very long time to reach terminal vacuum.
The idea is to get vacuum as good as possible and then isolate the gun chamber before turning on the pump. Even so, startup current would be high.
I've used the hair dryer most of the time. Then, sometimes whack the pump with the screwdriver handle. This is only for the small pumps. For some reason, they don't start as readily as the larger ones.
gary g.
At 09:48 AM 1/21/2006, you wrote:
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==============================Original Headers============================== 10, 20 -- From gary-at-gaugler.com Sat Jan 21 12:09:22 2006 10, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 10, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0LI9KuQ010814 10, 20 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 12:09:21 -0600 10, 20 -- Received: (qmail 22469 invoked from network); 21 Jan 2006 10:09:20 -0800 10, 20 -- Received: by simscan 1.1.0 ppid: 22466, pid: 22467, t: 0.1656s 10, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 10, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 10, 20 -- by qsmtp1 with SMTP; 21 Jan 2006 10:09:20 -0800 10, 20 -- Message-Id: {6.2.3.4.2.20060121100351.020356f8-at-mail.calweb.com} 10, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 10, 20 -- Date: Sat, 21 Jan 2006 10:09:22 -0800 10, 20 -- To: bigelow-at-engin.umich.edu 10, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 10, 20 -- Subject: Re: [Microscopy] RE: Starting Ion Pumps 10, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 10, 20 -- In-Reply-To: {200601211748.k0LHmBJV006678-at-ns.microscopy.com} 10, 20 -- References: {200601211748.k0LHmBJV006678-at-ns.microscopy.com} 10, 20 -- Mime-Version: 1.0 10, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Celebration of life for Delbert E. Philpott September 24, 1923 - December 11, 2005
Yesterday at the Liberty Lodge Masonic Center in Santa Clara, CA, we honored the life and friendship of Dr. Del Philpott, a lifelong microscopist and soldier who truly believed in the perpetuation of peace and worked for that goal his entire life.
His wife, Donna, agreed to our reprinting of a few of his many achievements which she listed in the memorial pamphlet.
Del was born and raised in Omro, Wisconsin. Dr. Philpott was a World War II Veteran who served with the Fighting 69th Infantry Division as they raced across Europe to link up with the Russian 58th Guards at the Elbe River in Germany in April of 1945. The famous link-up photo, by photographer Allan Jackson of 3 Americans (including Del), and 3 Russians shaking hands was printed in newspapers across the United States. The soldiers however didn't see the photo because the war hadn't yet ended. Fellow Infantryman Sam Popkins, who had helped Allan recruit soldiers for the photo, later told Del about it and confirmed that Del was one the soldiers in the photo. Del was awarded the Bronze Star and Purple Heart Medals for his service.
After the war, Del completed his Bachelor's Degree in chemistry at Indiana University in 1948 and his Master's Degree in 1949. As a Research Associate at the University of Illinois Medical School in Chicago from 1949 to 1952, he established the first electron microscope facility for the medical school. He was Head of Electron Microscopy, Marine Biological Lab and Institute for Muscle Research, Woods Hole, MA from 1952 to 1963, where he established the electron microscope facility for the 2 Institutes.
A pioneer in the field of Electron Microscopy, he directed and ran research projects under Nobel Laureate Dr. Albert Szent-Gyorgyi in the winter and for the Marine Biological Lab at Woods Hole during the summer. He built his own ultramicrotome for ultra-thin sectioning.
He published the first paper on ultrastructure of the human heart with Dr. Bruno Kish and published the first electron micrographs of a protein crystal isolated from muscle. He demonstrated that ribosomes travel from the nucleus to the cytoplasm via the nuclear pores. Del got his PhD in cytology in 1963 at Boston University.
While Professor of Biochemistry at the University of Colorado from 1963 to 1965, he established and ran the electron microscope laboratory at the medical school. In 1966, he was Head of the Department of Electron Microscopy and Co-Director of the Institute for Biomedical Research at Mercy Hospital in Denver, CO.
Del came to California in the 60's to work as a Research Scientist as the Head of the Ultrastructure Laboratory at the Moffett Field NASA Ames Research Center. He inspected lunar soil for signs of life and had experiments flown on both American and Russian spacecraft including Apollo 17, Cosmos 736, Cosmos 936, and SL-3. He developed a fixative for space flight that preserves the ultrastructure of tissues for over 4 years without the need for embedding. Del retired from NASA in 1990, but worked under contract with the Lockheed Martin Company at NASA Ames as a Research Associate whose duties included assisting visiting Russian scientists with space related experiments.
During his professional career, Del authored over 230 scientific papers and wrote articles for several books and magazines.
In 1995, Del and his wife Donna co-edited the book "Hands Across The Elbe", stories by American and Russian veterans about the link up that cut Fascist Germany in half in April 1945.
Del was one of 10 veterans chosen to be guests at the Russian government's May 9, 2005, 60th Anniversary Celebration of the end of World War II. He was selected to be seated at the table with the President of China, President Putin, and President and Mrs. Bush at the Kremlin Reception following the Parade e in Red Square.
Del's interests included flying, raising dogs, ham radio, ceramics, spinning, photography, woodcarving, magic, and travel. He held offices in many organizations including several scientific societies, veterans' organizations, and The Unique Boutique at the Sunnyvale Senior Center. He was a member of Liberty Lodge $299, Valley Star Chapter #250 O.E.S., and the San Jose Scottish Rite.
He wanted to be remembered for his part in developing interaction between the American and Russian scientists in space research. He believed that interactions among the citizens of all nations are necessary for the perpetuation of peace and felt that the "Spirit of the Elbe" encourages this vision.
Thank you Donna for putting together such an information-packed bibliography.
I knew Del from the late 60's from the microscopy society. He was a wonderful friend and had an uncanny sense of humor. I would like to share 2 of Del's favorite stories. And for those of you who knew Del, you know he had LOTS of stories.
I can no longer remember the exact year, but it was in the 70's. Someone sent in a bogus abstract for the EMSA meeting with a crazy title and crazy names of authors. Unfortunately I no longer remember those either. However the following year, as you can imagine, EMSA was trying to review the abstracts or at least look more carefully at the titles and authors. That year Del sent in an abstract about research on lunar samples. Well, whoever reviewed his abstract decided that no one could possibly be named Delbert Philpott and certainly no one was looking at moon samples, SO, his paper was rejected!!
Del's sense of humor was ever present. Before he knew Donna, again in the 70's sometime, Del was at the EMSA meeting telling us about a Valentine's present he got for his girlfriend. He bought some dog biscuits (yes dog biscuits), had them chocolate coated, put them in those fancy white wrappers, and put them in a standard Valentine's Candy Box. He gave them to his girlfriend. The next year I saw Del, I asked him how his girlfriend liked her Valentine's gift. He said "Well, I actually haven't seen her since then. I guess she didn't like that brand dog biscuits"!!!!
Del is gone, but his loving sense of humor, and desire for the promotion of peace through interactions among citizens of all nations will always be with us. We love you Del, and will miss you always.
God Bless Us All, Judy Murphy
==============================Original Headers============================== 21, 17 -- From murphyjudy-at-comcast.net Sun Jan 22 17:23:21 2006 21, 17 -- Received: from sccrmhc11.comcast.net (sccrmhc11.comcast.net [63.240.77.81]) 21, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0MNNK1W018752 21, 17 -- for {Microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 17:23:21 -0600 21, 17 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 21, 17 -- by comcast.net (sccrmhc11) with ESMTP 21, 17 -- id {2006012223231901100qjb1ie} ; Sun, 22 Jan 2006 23:23:19 +0000 21, 17 -- Message-ID: {43D413E6.6010102-at-comcast.net} 21, 17 -- Date: Sun, 22 Jan 2006 15:23:18 -0800 21, 17 -- From: Judy Murphy {murphyjudy-at-comcast.net} 21, 17 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 21, 17 -- X-Accept-Language: en-us, en 21, 17 -- MIME-Version: 1.0 21, 17 -- To: Microscopy {Microscopy-at-microscopy.com} 21, 17 -- Subject: We Remember Del Philpott 21, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 21, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Gary, I have been working with LR white and have been facing similar problems. They are more so only when I immuno label those sections. Then again my sections are more than 2 mm in size and they are thin sections. I have managed to reduce the wrinkles by changing the washing techniques between immunolabelling steps but that hasnt completely eliminated the wrinkles. I have observed that LR white section around 1mm wide didnt have so many wrinkles. Unfortunately for me I cant have thicker sections or smaller sections to avoid this problem. I cannot use a different resin since it interfers with my immunolabeling. SO the bottom line being, I have learnt to live with it unfortunately. But I have a feeling that smaller sections do better and the wrinsing the grids in a drop of water/ buffer on a petridish does help reduce the wrinkles greatly if not eliminate it.
Any advice on photoshop micracles of ironing out the wrinkles would be appreciated. I hope facelifting TEM pics will not be frowned upon by the microscopy community.
Looking forward for comments and suggestion.
Regards, Vinod Nair Graduate Student Dept. of Biology New Mexico State University
On 1/21/06, W.Muss-at-salk.at {W.Muss-at-salk.at} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning, all, } } Dear Garry, } } in my experience (I am familiar with large semithin sections = up to 5 x 5 } mm from human diagnostic samples for some 20 years now) your question } cannot be answered with one sentence. } There are several parameters to be included in a thoroughly checking of } causes, starting from the type of tissue, via fixation, (full) dehydration, } intermedium (full evaporation/complete exchange by resin), type, quality } [polymerisation, hardness] of resin, quality of knife edge, sectioning } parameters (knife, cutting angle, speed) and type of transfer of sections } from the water trough to the slide, and last but not least, "special } treatment" of the floating section on a water drop (i.e., for example, } trying to spread sections by xylene vapor [other - more healthier - } vapors?] or gentle - longer - warming up by means of a light bulb } positioned above the section/slide). } So IMO, you have to discriminate the problem(s) from the whole processing } schedule.......(;-(..... } } You tell us that you face problems not everytime, but sometimes..... } Could you give us some examples (tissue type, some hints on chemicals } /resin you use?) where you get such results? (not to forget the dimensions } of the tissue blocks and type of knife used).... } } Perhaps there is a simple solution......who knows, } } } best regards and have a nice weekend, } } Wolfgang Muss } } Salzburg, Austria } } Paracelsus Medical Private University (PMU) } Institute of Pathology } Electron Microscopy Lab } Muellner Hauptstrasse 48 } A-5020 SALZBURG, Austria/Europe } Phone work: +43+662+4482+4720 } Mobile phone work:+43+662+4482-57704 } Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o } W.Muss") } E-Mail work: W.Muss-at-SALK.at } } Mobile-phone private: ++43+676+5 369-456 } E-Mail private: wij.Muss-at-aon.at } ------------------------------------------------------------------------ } --------------------------------Information on behalf of } Society for Cutaneous Ultrastructure Research (SCUR) } PLEASE VISIT THE UPDATED WEBSITE of SCUR at } } http://www.scur.org { } ------------------------------------------------------------------------- } Forthcoming Meetings: } 33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland } WEBSITE, containing all FORMS: http://www.scur.org.pl } Additional informations: send an E-Mail } kwoznia-at-amwaw.edu.pl } ---------------------- } 34th Annual Meeting of the SCUR, 11th-12th May, 2007, PRAGUE } Czech Republic } ---------------------- } 35th Annual Meeting of the SCUR, 11th -12th May, 2008, NARA or KYOTO, Japan } Joint Meeting with the } JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology } } } } } } } ---------- } Von: GBurgess-at-exchange.hsc.mb.ca[SMTP:GBurgess-at-exchange.hsc.mb.ca] } Antwort an: GBurgess-at-exchange.hsc.mb.ca } Gesendet: Freitag, 20. Janner 2006 23:52 } An: W.Muss-at-salk.at } Betreff: [Microscopy] Wrinkled LM Sections } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } Even though I am very experienced in Electron Microscopy, there are times } when I cut LM sections that are extremely wrinkled, and they look like } cracked glass. I normally don't experience this, but when it happens, even } considering every possible variable, I'm still not exactly sure what might } be at work here to give this sort of result. } } I'm looking for ideas here as to what might be causing this effect. What } do } people think? } } } Garry Burgess } Charge Technologist - Electron Microscopy } Health Sciences Centre } Winnipeg, Canada. } } This e-mail and/or any documents in this transmission is intended for the } address(s) only and may contain legally privileged or confidential } information. Any unauthorized use, disclosure, distribution, copying or } dissemination is strictly prohibited. If you receive this transmission in } error, please notify the sender immediately and return the original. } } ==============================Original } Headers============================== } 6, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Jan 20 16:10:02 2006 } 6, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca } [142.233.100.122]) } 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k0KMA1mn017769 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Jan 2006 16:10:01 -0600 } 6, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) } by } 6, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id } 6, 20 -- {B0017584293-at-hscxntmx0003.hsc.mb.ca} for } {Microscopy-at-microscopy.com} ;Fri, } 6, 20 -- 20 Jan 2006 16:09:53 -0600 } 6, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service } 6, 20 -- (5.5.2653.19)id {T7CLC1WX} ; Fri, 20 Jan 2006 16:10:27 -0600 } 6, 20 -- Message-ID: } 6, 20 -- {00A937989100304A83A058F6C45873FF32A36C-at-hscxntmx0005.hsc.mb.ca} } 6, 20 -- Date: Fri, 20 Jan 2006 16:06:48 -0600 } 6, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} } 6, 20 -- Subject: Wrinkled LM Sections } 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 6, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- Content-Type: text/plain; } 6, 20 -- charset="iso-8859-1" } ==============================End of - } Headers============================== } } } } ==============================Original Headers============================== } 25, 28 -- From W.Muss-at-salk.at Sat Jan 21 04:43:58 2006 } 25, 28 -- Received: from Hermes.salk.at (hermes.lks.at [193.170.167.9]) } 25, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0LAhv9E003479 } 25, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 21 Jan 2006 04:43:57 -0600 } 25, 28 -- Received: from localhost (localhost [127.0.0.1]) } 25, 28 -- by hermes.lks.at (Postfix) with ESMTP id B185A5A9046; } 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) } 25, 28 -- Received: from hermes.lks.at ([127.0.0.1]) } 25, 28 -- by localhost (hermes.lks.local [127.0.0.1]) (amavisd-new, port 10024) } 25, 28 -- with ESMTP id 42900-09; Sat, 21 Jan 2006 11:43:51 +0100 (CET) } 25, 28 -- Received: from c1pa003 (unknown [192.168.42.3]) } 25, 28 -- by hermes.lks.at (Postfix) with SMTP id 754D65A9044; } 25, 28 -- Sat, 21 Jan 2006 11:43:51 +0100 (CET) } 25, 28 -- Received: by localhost with Microsoft MAPI; Sat, 21 Jan 2006 11:43:49 +0100 } 25, 28 -- Message-ID: {01C61E7F.F216D3E0.W.Muss-at-salk.at} } 25, 28 -- From: MUSS Wolfgang PhD {W.Muss-at-salk.at} } 25, 28 -- Reply-To: "W.Muss-at-salk.at" {W.Muss-at-salk.at} } 25, 28 -- To: "'GBurgess-at-exchange.hsc.mb.ca'" {GBurgess-at-exchange.hsc.mb.ca} , } 25, 28 -- "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 25, 28 -- Subject: [Microscopy] Re: Wrinkled LM Sections } 25, 28 -- Date: Sat, 21 Jan 2006 11:43:48 +0100 } 25, 28 -- Return-Receipt-To: MUSS Wolfgang PhD {W.Muss-at-salk.at} } 25, 28 -- Organization: SALK,Salzburger Landeskliniken, Pathologie, EM-Labor } 25, 28 -- X-Mailer: Microsoft Internet E-Mail/MAPI - 8.0.0.4211 } 25, 28 -- MIME-Version: 1.0 } 25, 28 -- Content-Type: text/plain; charset="us-ascii" } 25, 28 -- Content-Transfer-Encoding: 7bit } 25, 28 -- X-Virii-Scanned: by Sophos/ClamAV at salk.at } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 25 -- From nairvinods-at-gmail.com Sun Jan 22 17:45:05 2006 6, 25 -- Received: from zproxy.gmail.com (zproxy.gmail.com [64.233.162.195]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0MNj4D3022636 6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 17:45:04 -0600 6, 25 -- Received: by zproxy.gmail.com with SMTP id 9so805621nzo 6, 25 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 15:45:03 -0800 (PST) 6, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 25 -- s=beta; d=gmail.com; 6, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 6, 25 -- b=iwLI/yOhyn8RBFdLsd4GrjjADMj8yG6x8a+cZRddAPXP313KWFTH8mhP3WJuqdwmBf56pLTJ6QHi6KG7BMIsRtxOEYY4fvwY5oFvcx+g2Qe17GhH6Q6gSFUGuaU44HZYNriuBxtZcmHsPNQ9H4Cu/ddrt5xiiMyNw1HrJ33u1+I= 6, 25 -- Received: by 10.64.251.4 with SMTP id y4mr2436900qbh; 6, 25 -- Sun, 22 Jan 2006 15:38:52 -0800 (PST) 6, 25 -- Received: by 10.64.201.14 with HTTP; Sun, 22 Jan 2006 15:38:52 -0800 (PST) 6, 25 -- Message-ID: {ea42a3900601221538n63c2c777pab547602772aa678-at-mail.gmail.com} 6, 25 -- Date: Sun, 22 Jan 2006 16:38:52 -0700 6, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 6, 25 -- To: microscopy-at-microscopy.com 6, 25 -- Subject: Re: [Microscopy] Re: Wrinkled LM Sections 6, 25 -- In-Reply-To: {200601211126.k0LBQBcT011510-at-ns.microscopy.com} 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=UTF-8 6, 25 -- Content-Disposition: inline 6, 25 -- References: {200601211126.k0LBQBcT011510-at-ns.microscopy.com} 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k0MNj4D3022636 ==============================End of - Headers==============================
Mei Lie, Making formvar films in ethylene dichloride We used these films for 30 yrs and controlled the thickness mostly by the percentage of formvar to ethylene dichloride. What percentage are you using?
Judy
Judy Murphy, PhD Microscopy Training, Imaging, and Lab Design Stockton, CA 95219 murphyjudy-at-comcast.net
wong-at-msg.ucsf.edu wrote:
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==============================Original Headers============================== 12, 19 -- From murphyjudy-at-comcast.net Sun Jan 22 22:53:43 2006 12, 19 -- Received: from sccrmhc12.comcast.net (sccrmhc12.comcast.net [63.240.77.82]) 12, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0N4rhWv009022 12, 19 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 22:53:43 -0600 12, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 12, 19 -- by comcast.net (sccrmhc12) with ESMTP 12, 19 -- id {20060123045340012005vqqbe} ; Mon, 23 Jan 2006 04:53:41 +0000 12, 19 -- Message-ID: {43D46116.2070908-at-comcast.net} 12, 19 -- Date: Sun, 22 Jan 2006 20:52:38 -0800 12, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 12, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 12, 19 -- X-Accept-Language: en-us, en 12, 19 -- MIME-Version: 1.0 12, 19 -- To: Microscopy List Server {microscopy-at-microscopy.com} 12, 19 -- Subject: Re: [Microscopy] viaWWW: Making formvar thicker 12, 19 -- References: {200601211425.k0LEP0O9019151-at-ns.microscopy.com} 12, 19 -- In-Reply-To: {200601211425.k0LEP0O9019151-at-ns.microscopy.com} 12, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
A web site was brought to my attention that is a collection of photographs by him (and several of him) during Del's time with Albert Szent-Gyorgyi. I found it very interesting and rich in the history of science.
Thank you Peter for sharing the information AND hold on to that signed copy of Crossing the Elbe!
murphyjudy-at-comcast.net wrote:
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==============================Original Headers============================== 8, 19 -- From murphyjudy-at-comcast.net Sun Jan 22 23:08:21 2006 8, 19 -- Received: from sccrmhc14.comcast.net (sccrmhc14.comcast.net [63.240.77.84]) 8, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0N58KXw011747 8, 19 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 23:08:20 -0600 8, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 8, 19 -- by comcast.net (sccrmhc14) with ESMTP 8, 19 -- id {2006012305081301400kjrige} ; Mon, 23 Jan 2006 05:08:14 +0000 8, 19 -- Message-ID: {43D464BD.4010206-at-comcast.net} 8, 19 -- Date: Sun, 22 Jan 2006 21:08:13 -0800 8, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 8, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 8, 19 -- X-Accept-Language: en-us, en 8, 19 -- MIME-Version: 1.0 8, 19 -- To: Microscopy List Server {microscopy-at-microscopy.com} 8, 19 -- Subject: Re: [Microscopy] We Remember Del Philpott 8, 19 -- References: {200601222350.k0MNoOo1024081-at-ns.microscopy.com} 8, 19 -- In-Reply-To: {200601222350.k0MNoOo1024081-at-ns.microscopy.com} 8, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi: I am restoring a Zeiss 109 TEM where the Leybold IZ-80 iongetter pump has been removed, however they left the magnets. The pump does not have to be in working condition. If you have on lying around and are willing to part with it or sell it please let me know. Peter Jordan/EMSI
==============================Original Headers============================== 2, 22 -- From pjordan-at-dslextreme.com Sun Jan 22 23:53:27 2006 2, 22 -- Received: from mail5.dslextreme.com (mail5.dslextreme.com [66.51.199.81]) 2, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0N5rQiS023856 2, 22 -- for {microscopy-at-msa.microscopy.com} ; Sun, 22 Jan 2006 23:53:26 -0600 2, 22 -- Received: (qmail 30488 invoked from network); 23 Jan 2006 05:52:12 -0000 2, 22 -- Received: from unknown (HELO DCPZ5N31) (68.183.94.207) 2, 22 -- by mail5.dslextreme.com with SMTP; Sun, 22 Jan 2006 21:52:12 -0800 2, 22 -- Message-ID: {005401c61fe1$5218af90$cf5eb744-at-DCPZ5N31} 2, 22 -- From: "Peter Jordan" {pjordan-at-dslextreme.com} 2, 22 -- To: "Electron Microscopy" {Microscopy-at-msa.microscopy.com} 2, 22 -- Subject: Iongetter pump for Zeiss 109 2, 22 -- Date: Sun, 22 Jan 2006 21:53:22 -0800 2, 22 -- MIME-Version: 1.0 2, 22 -- Content-Type: text/plain; 2, 22 -- format=flowed; 2, 22 -- charset="iso-8859-1"; 2, 22 -- reply-type=original 2, 22 -- Content-Transfer-Encoding: 7bit 2, 22 -- X-Priority: 3 2, 22 -- X-MSMail-Priority: Normal 2, 22 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 2, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Dear Gary, Let me begin with apologising for not reading the email correctly. You can see what I have been pondering over from my reply with regards to LR white resin:( Guess it was desperation that made me misread LM as LR and link it to the wrinkeling problems I have been facing. Having said that I feel very foolish now for being hasty and replying to your email query. My sincere apologies.
But then again I am open for suggestions,comments or insight into the problem I am facing. regards, Vinod
==============================Original Headers============================== 3, 25 -- From nairvinods-at-gmail.com Mon Jan 23 00:03:21 2006 3, 25 -- Received: from wproxy.gmail.com (wproxy.gmail.com [64.233.184.196]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0N63JfS026967 3, 25 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2006 00:03:20 -0600 3, 25 -- Received: by wproxy.gmail.com with SMTP id i30so857046wra 3, 25 -- for {microscopy-at-microscopy.com} ; Sun, 22 Jan 2006 22:03:19 -0800 (PST) 3, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 25 -- s=beta; d=gmail.com; 3, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 25 -- b=qLDCg7ZEROXSfD8aXh3sQ7gV08fkTVY98jzE998c6AOreIritxcbW8wfosf9yDu9vRIfZK7mOZoljeb0to6Wf+TmtvFGfObfREqnJAWvRbc9PTSIm6Ow+fqo0HEEIcqZzzbUyMrn61hqiKideomsMmSKQsDqs0bm8+Hne14//ZM= 3, 25 -- Received: by 10.64.143.19 with SMTP id q19mr271269qbd; 3, 25 -- Sun, 22 Jan 2006 21:57:15 -0800 (PST) 3, 25 -- Received: by 10.64.201.14 with HTTP; Sun, 22 Jan 2006 21:57:15 -0800 (PST) 3, 25 -- Message-ID: {ea42a3900601222157v6fe56138p3a4d08b1e4b0777a-at-mail.gmail.com} 3, 25 -- Date: Sun, 22 Jan 2006 22:57:15 -0700 3, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 3, 25 -- To: microscopy-at-microscopy.com 3, 25 -- Subject: Re: [Microscopy] Wrinkled LM Sections 3, 25 -- In-Reply-To: {200601230040.k0N0elR9004151-at-ns.microscopy.com} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; charset=UTF-8 3, 25 -- Content-Disposition: inline 3, 25 -- References: {200601230040.k0N0elR9004151-at-ns.microscopy.com} 3, 25 -- Content-Transfer-Encoding: 8bit 3, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k0N63JfS026967 ==============================End of - Headers==============================
On Jan 21, 2006, at 6:03 AM, wong-at-msg.ucsf.edu wrote:
} Question: When using formvar in ethylene dichloride, is the any way to } make the films slightly thicker. I know when using formvar in } chloroform, you can just drain slower or faster to make films thinner } or thicker. Is there something like this for the ethylene dichloride } formula? I have tried several different times but so far haven't hit } on anything totally satisfactory. } Dear Mei Lie, Using a more concentrated formvar solution should do it, and if you only have the pre-disolved formvar, you could try either letting some of the solvent evaporate or double dipping. For finer variations you might try letting the formvar drain at a shallower angle, which will drain it slightly slower. Good luck. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Mon Jan 23 11:37:40 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NHbemg016835 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 23 Jan 2006 11:37:40 -0600 4, 22 -- Received: from localhost (fire-dog [192.168.1.4]) 4, 22 -- by earth-ox-postvirus (Postfix) with ESMTP id 7071C109F3B 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 23 Jan 2006 09:37:39 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by earth-ox.its.caltech.edu (Postfix) with ESMTP id E6173109E19 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 23 Jan 2006 09:37:37 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200601211403.k0LE3NrZ014761-at-ns.microscopy.com} 4, 22 -- References: {200601211403.k0LE3NrZ014761-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {6675a89478c5c6f5c36fc2d5e8dcd7a0-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: Making formvar thicker 4, 22 -- Date: Mon, 23 Jan 2006 09:44:45 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Dear All, We have an older Zeiss Opni-1 surgical microscope and we'd like to replace its broken tungsten illuminator with a fiber optic illuminator. this would preserve the large illuminated field diameter and be adjustable in angle. the usual web searches have shown that retrofits were made for this scope, but haven't provided any useful information on performance or options. It is already used in conjunction with bifurcated light guides, but we have users who need the stock style of illumination.
Thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-u.washington.edu
************************************************************************ ****** The box said "Requires Windows 95 or better", so I bought a Macintosh. ************************************************************************ ******
==============================Original Headers============================== 7, 23 -- From glenmac-at-u.washington.edu Mon Jan 23 11:39:38 2006 7, 23 -- Received: from mxout2.cac.washington.edu (mxout2.cac.washington.edu [140.142.33.4]) 7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NHdbYl017204 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 11:39:37 -0600 7, 23 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.139]) 7, 23 -- by mxout2.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NHdaNw018071 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 09:39:37 -0800 7, 23 -- X-Auth-Received: from [128.95.178.71] ([128.95.178.71]) 7, 23 -- (authenticated authid=glenmac) 7, 23 -- by smtp.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NHdac2004853 7, 23 -- (version=TLSv1/SSLv3 cipher=RC4-SHA bits=128 verify=NOT) 7, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 09:39:36 -0800 7, 23 -- Mime-Version: 1.0 (Apple Message framework v746.2) 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- Message-Id: {E3E326B4-892B-4DAE-BA4A-8D7CA81A632C-at-u.washington.edu} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 23 -- To: MSA {Microscopy-at-MSA.Microscopy.Com} 7, 23 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 7, 23 -- Subject: fiber illuminator for Opni-1 7, 23 -- Date: Mon, 23 Jan 2006 09:39:32 -0800 7, 23 -- X-Mailer: Apple Mail (2.746.2) 7, 23 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0, __STOCK_PHRASE_6 0' ==============================End of - Headers==============================
I need to measure thermal conductivity of thin, 50-200 nm film. I would appreciate any suggestions regarding to equipment, techniques, and literature references.
Thank you,
Victoria Fink vfink-at-shaw.ca
==============================Original Headers============================== 7, 28 -- From vfink-at-shaw.ca Mon Jan 23 12:04:15 2006 7, 28 -- Received: from pd5mo1so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10]) 7, 28 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NI4FmR023679 7, 28 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 12:04:15 -0600 7, 28 -- Received: from pd2mr7so.prod.shaw.ca (pd2mr7so-qfe3.prod.shaw.ca [10.0.141.10]) 7, 28 -- by l-daemon (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 7, 28 -- with ESMTP id {0ITK00H9N4UXI290-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 7, 28 -- Mon, 23 Jan 2006 11:04:09 -0700 (MST) 7, 28 -- Received: from pn2ml1so.prod.shaw.ca ([10.0.121.145]) 7, 28 -- by pd2mr7so.prod.shaw.ca (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 7, 28 -- 15 2004)) with ESMTP id {0ITK00JKF4UX7300-at-pd2mr7so.prod.shaw.ca} for 7, 28 -- Microscopy-at-MSA.Microscopy.Com; Mon, 23 Jan 2006 11:04:09 -0700 (MST) 7, 28 -- Received: from homep5whcwsdwx ([70.68.254.15]) 7, 28 -- by l-daemon (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 7, 28 -- with SMTP id {0ITK001EW4UVGR10-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 7, 28 -- Mon, 23 Jan 2006 11:04:09 -0700 (MST) 7, 28 -- Date: Mon, 23 Jan 2006 10:04:11 -0800 7, 28 -- From: Victoria Fink {vfink-at-shaw.ca} 7, 28 -- Subject: thermal conductivity of thin film measurement techniques 7, 28 -- To: MSA listserver {Microscopy-at-MSA.Microscopy.Com} 7, 28 -- Message-id: {042001c62047$69d10270$0ffe4446-at-homep5whcwsdwx} 7, 28 -- MIME-version: 1.0 7, 28 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 7, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 7, 28 -- Content-type: text/plain; format=flowed; charset=iso-8859-1; reply-type=response 7, 28 -- Content-transfer-encoding: 7bit 7, 28 -- X-Priority: 3 7, 28 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
Inked to measure thermal conductivity of thin, 50-200 nm film. I would appreciate any suggestions regarding to equipment, techniques, and literature references. Thank you,
Victoria Fink vfink-at-shaw.ca
==============================Original Headers============================== 6, 29 -- From vfink-at-shaw.ca Mon Jan 23 12:04:23 2006 6, 29 -- Received: from pd4mo1so.prod.shaw.ca (shawidc-mo1.cg.shawcable.net [24.71.223.10]) 6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NI4L6j023721 6, 29 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 12:04:22 -0600 6, 29 -- Received: from pd3mr2so.prod.shaw.ca 6, 29 -- (pd3mr2so-qfe3.prod.shaw.ca [10.0.141.178]) by l-daemon 6, 29 -- (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 6, 29 -- with ESMTP id {0ITK00MFH4TCBF80-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 6, 29 -- Mon, 23 Jan 2006 11:03:12 -0700 (MST) 6, 29 -- Received: from pn2ml1so.prod.shaw.ca ([10.0.121.145]) 6, 29 -- by pd3mr2so.prod.shaw.ca (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 6, 29 -- 15 2004)) with ESMTP id {0ITK00BYS4TCXT10-at-pd3mr2so.prod.shaw.ca} for 6, 29 -- Microscopy-at-MSA.Microscopy.Com; Mon, 23 Jan 2006 11:03:12 -0700 (MST) 6, 29 -- Received: from homep5whcwsdwx ([70.68.254.15]) 6, 29 -- by l-daemon (Sun ONE Messaging Server 6.0 HotFix 1.01 (built Mar 15 2004)) 6, 29 -- with SMTP id {0ITK00FRN4TAXA50-at-l-daemon} for Microscopy-at-MSA.Microscopy.Com; 6, 29 -- Mon, 23 Jan 2006 11:03:12 -0700 (MST) 6, 29 -- Date: Mon, 23 Jan 2006 10:03:14 -0800 6, 29 -- From: Victoria Fink {vfink-at-shaw.ca} 6, 29 -- Subject: thermal conductivity of thin film measurement techniques 6, 29 -- To: MSA listserver {Microscopy-at-MSA.Microscopy.Com} 6, 29 -- Message-id: {041a01c62047$47e848d0$0ffe4446-at-homep5whcwsdwx} 6, 29 -- MIME-version: 1.0 6, 29 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 6, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 6, 29 -- Content-type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original 6, 29 -- Content-transfer-encoding: 7bit 6, 29 -- X-Priority: 3 6, 29 -- X-MSMail-priority: Normal ==============================End of - Headers==============================
A number of our customers for coated grids request a variety of thicknesses. What we do for thicker coated grids is increase the percentage of formvar in ethylene dichloride.
Mike Bouchard
Disclaimer: Ladd Research sells Formvar coated grids and the essentials to make your own.
At 09:46 AM 1/21/2006, wong-at-msg.ucsf.edu wrote:
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I am curious about the possible skinkage artifact that may or may not occur when cryoprotecting lightly fixed (4% para / 0.1% glut) watery tissues (embryonic skin) in 20% PVP / 1.7M sucrose for ultrathin cryomicrotomy? Does the cryoprotectant replace the water in a controlled way and keep the tissue fat and happy or does it draw the water out and collapse the tissue? I would love any information on this subject or a possible reference.
Robert Underwood University of Washington Dermatology
==============================Original Headers============================== 4, 20 -- From underwoo-at-u.washington.edu Mon Jan 23 13:08:13 2006 4, 20 -- Received: from mxout7.cac.washington.edu (mxout7.cac.washington.edu [140.142.32.178]) 4, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NJ8Anx013006 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 13:08:11 -0600 4, 20 -- Received: from hymn02.u.washington.edu (hymn02.u.washington.edu [140.142.13.239]) 4, 20 -- by mxout7.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NJ89DN018919 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 11:08:09 -0800 4, 20 -- Received: from localhost (localhost [127.0.0.1]) 4, 20 -- by hymn02.u.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k0NJ894u023894 4, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Mon, 23 Jan 2006 11:08:09 -0800 4, 20 -- X-Auth-Received: from [128.208.106.163] by hymn02.u.washington.edu via HTTP; Mon, 23 Jan 2006 11:08:09 PST 4, 20 -- Date: Mon, 23 Jan 2006 11:08:09 -0800 (PST) 4, 20 -- From: Robert A Underwood {underwoo-at-u.washington.edu} 4, 20 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} 4, 20 -- Subject: [Microscopy] Cryoprotection causing shrinkage artifact 4, 20 -- Message-ID: {Pine.LNX.4.43.0601231108090.3305-at-hymn02.u.washington.edu} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 4, 20 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' ==============================End of - Headers==============================
Postdoctoral Position, Department of Materials Science and Engineering Lehigh University,
Available March 2006. Opening for post-doc to work on project involving processing of patterned sapphire substrates via oxidation and solid state conversion of a metallic Al coating. Person would be responsible for processing and EBSD/TEM characterization of both the substrates, together with TEM of MOCVD grown GaN layers to determine the defect density. Expertise in materials processing and electron microscopy required, experience with thin film processing a plus. Please forward resume by e-mail to Prof. Helen Chan (Helen.Chan-at-lehigh.edu) and / or Prof. Richard Vinci (rpv2-at-lehigh.edu).
...........
Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195
==============================Original Headers============================== 5, 22 -- From jae5-at-lehigh.edu Mon Jan 23 13:25:44 2006 5, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0NJPhoS019690 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2006 13:25:44 -0600 5, 22 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 5, 22 -- (authenticated bits=0) 5, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k0NJPhEc031735 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 5, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2006 14:25:43 -0500 5, 22 -- Message-ID: {43D52DB7.9050707-at-lehigh.edu} 5, 22 -- Date: Mon, 23 Jan 2006 14:25:43 -0500 5, 22 -- From: Alwyn Eades {jae5-at-lehigh.edu} 5, 22 -- Organization: Lehigh University 5, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 5, 22 -- X-Accept-Language: en-us, en 5, 22 -- MIME-Version: 1.0 5, 22 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 5, 22 -- Subject: Post doctoral position at Lehigh 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 5, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I have a colleague who recently experienced some down time on his SEM. The system went through repeated cycles of pump down, and venting and was eventually left powered down while waiting on a computer replacement.
After getting everything operating again, an oil film was found that had built up on the x-ray detector thin window. This was initially causing an unacceptable amount of background in the 0-3kV range of the x-ray signal as well as an overall low count rate. The collimator was removed and cleaned, which corrected the noise and count rate. They are in the process of replacing the roughing lines and cleaning the chamber to prevent further contamination. Now, when calibrating with a copper standard, the ratio of the L line signal versus the K lines is dramatically favoring the L. This was not the case before.
Any ideas why this might be happening and how to correct it?
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Tue Jan 24 09:02:19 2006 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OF2JlW001861 5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 09:02:19 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Tue, 24 Jan 2006 10:02:19 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: SEM x-ray peak ratios 5, 23 -- Date: Tue, 24 Jan 2006 10:02:18 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3585FA-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: SEM x-ray peak ratios 5, 23 -- Thread-Index: AcYg9yuMm942rGb1QfKCevfjxyMD/g== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 24 Jan 2006 15:02:19.0222 (UTC) FILETIME=[2BE72F60:01C620F7] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0OF2JlW001861 ==============================End of - Headers==============================
Does anyone remember a quick and easy way to calibrate - it has what 25 taps - so if anyone remembers how to do the calculations, please let me know ASAP. Thanks Barbara
==============================Original Headers============================== 1, 20 -- From maloneyb-at-fiu.edu Tue Jan 24 13:40:30 2006 1, 20 -- Received: from imappopsmtp.fiu.edu (imappopsmtp.fiu.edu [131.94.74.203]) 1, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OJeTel015388 1, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Tue, 24 Jan 2006 13:40:30 -0600 1, 20 -- Received: from [131.94.95.156] by imappopsmtp.fiu.edu 1, 20 -- (InterMail vK.4.04.00.00 201-232-137 license dcade68804da15b88628cbdb48d26a54) 1, 20 -- with SMTP 1, 20 -- id {20060124194058.VXOU22222.imappopsmtp-at-[131.94.95.156]} 1, 20 -- for {microscopy-at-sparc5.microscopy.com} ; 1, 20 -- Tue, 24 Jan 2006 14:40:58 -0500 1, 20 -- Message-ID: {43D68175.1080106-at-fiu.edu} 1, 20 -- Date: Tue, 24 Jan 2006 14:35:17 -0500 1, 20 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 1, 20 -- X-Accept-Language: en-us, en 1, 20 -- MIME-Version: 1.0 1, 20 -- To: "'microscopy-at-msa.microscopy.com'" {microscopy-at-ns.microscopy.com} 1, 20 -- Subject: Phillips 300 TEM calibration 1, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed 1, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have had a request from a user looking for a cryo- ultramicrotomy workshop. Is anyone offering one in the next year or know of one? Thanks!
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 5, 23 -- From edelmare-at-muohio.edu Tue Jan 24 14:11:13 2006 5, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OKBBkX020625 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 14:11:12 -0600 5, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 5, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0OKB14j016596 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 15:11:01 -0500 5, 23 -- Received: from emf03 ([134.53.14.97]) 5, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0OKB002022369 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 15:11:00 -0500 5, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 5, 23 -- To: microscopy-at-Microscopy.com 5, 23 -- Date: Tue, 24 Jan 2006 15:11:49 -0500 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-type: text/plain; charset=US-ASCII 5, 23 -- Content-transfer-encoding: 7BIT 5, 23 -- Subject: Cryo-ultramicrotomy workshop? 5, 23 -- Message-ID: {43D643B5.14764.643E209-at-localhost} 5, 23 -- Priority: normal 5, 23 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 5, 23 -- X-Real-ConnectIP: 134.53.14.97 5, 23 -- X-Scanned-By: MIMEDefang 2.45 5, 23 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 ==============================End of - Headers==============================
Several years ago, Emispec Systems was purchased by FEI Company of Hillsboro, OR. Recently I've been contacted by several customers who in the past had purchased these data acquisition systems for their EM's and stated they are having difficulty making contact with the responsible person or department at FEI for upgrades and maintenance support.
I'm wondering if parties who are present owners of Emispec ES Vision systems have had success in seeking this support and can provide contact information or advise of appropriate channels to go through to obtain system support.
Please respond off list if you can shed some light on this.
Regards,
Bob Roberts EM Lab Services, Inc. 449 NW 62nd St. Topeka, Kansas 66617-1780 785.246.1232 voice 785.246.0168 fax www.emlabservices.com
==============================Original Headers============================== 8, 23 -- From emlabservices-at-cox.net Tue Jan 24 16:34:05 2006 8, 23 -- Received: from centrmmtao05.cox.net (centrmmtao05.cox.net [70.168.83.79]) 8, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OMY4Mt004289 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 16:34:05 -0600 8, 23 -- Received: from EMLabServices ([24.255.213.54]) by centrmmtao05.cox.net 8, 23 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP 8, 23 -- id {20060124223407.SQDC5868.centrmmtao05.cox.net-at-EMLabServices} 8, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 17:34:07 -0500 8, 23 -- Message-ID: {012701c62136$49ad42c0$6400a8c0-at-EMLabServices} 8, 23 -- From: "EM Lab Services" {emlabservices-at-cox.net} 8, 23 -- To: {Microscopy-at-microscopy.com} 8, 23 -- Subject: Emispec Systems/FEI Support? 8, 23 -- Date: Tue, 24 Jan 2006 15:34:06 -0700 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- format=flowed; 8, 23 -- charset="iso-8859-1"; 8, 23 -- reply-type=original 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- X-Priority: 3 8, 23 -- X-MSMail-Priority: Normal 8, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.2180 8, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (fmalherbe-at-swin.edu.au) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 24, 2006 at 18:23:45 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sallwein-at-ncifcrf.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Employment Opportunity to work in Support of the National Cancer Institute at SAIC-Frederick, Inc.
Question: SAIC-Frederick, Inc., working in support of the National Cancer Institute at Frederick, seeks an experienced Research Associate to be responsible for independent imaging TEM and SEM samples in a core facility, processing samples and coordinating GLP samples in the lab, processing, sectioning and imaging core samples, training, and conduct collaborative projects with the National Cancer Institute and the National Institutes of Health. Position requires a BS degree and at least four years of experience with TEM and/or SEM sample processing and imaging.
Please visit our web site at http://saic.ncifcrf.gov and refer to opportunity KMB134306 to apply on line.
==============================Original Headers============================== 7, 13 -- From zaluzec-at-microscopy.com Wed Jan 25 07:34:03 2006 7, 13 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 13 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PDY1FL024339 7, 13 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 07:34:03 -0600 7, 13 -- Mime-Version: 1.0 7, 13 -- X-Sender: (Unverified) 7, 13 -- Message-Id: {p06110406bffd2eaa5f0c-at-[206.69.208.22]} 7, 13 -- Date: Wed, 25 Jan 2006 07:34:00 -0600 7, 13 -- To: microscopy-at-microscopy.com 7, 13 -- From: sallwein-at-ncifcrf.gov (by way of MicroscopyListserver) 7, 13 -- Subject: [Filtered] MicroscopyListserverviaWWW: Employment Opportunity to 7, 13 -- work in Support of the National Cancer Institute at SAIC-Frederick 7, 13 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
The 5th International Cryo-EM Course is I believe June 6-17, 2006 in Vancouver, Canada at University of British Columbia by Dr. Elaine Humphrey from UBC and Dr. Kent McDonald, from UC, Berkeley with international speakers. This is an excellent course and caters to the needs of the particular students. Be sure to check with Elaine about dates. For more info email Dr. Elaine Humphrey ech-at-interchange.ubc.ca
Information from the previous course is listed at http://www.emlab.ubc.ca/CryoEM2005/index.html
Judy
Judy Murphy, PhD Microscopy Training, Imaging, and Lab Design Stockton, CA 95219 murphyjudy-at-comcast.net
edelmare-at-muohio.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 19 -- From murphyjudy-at-comcast.net Wed Jan 25 09:51:50 2006 14, 19 -- Received: from sccrmhc14.comcast.net (sccrmhc14.comcast.net [63.240.77.84]) 14, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PFpnJP012033 14, 19 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 09:51:50 -0600 14, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 14, 19 -- by comcast.net (sccrmhc14) with ESMTP 14, 19 -- id {2006012515514401400eiimne} ; Wed, 25 Jan 2006 15:51:44 +0000 14, 19 -- Message-ID: {43D79E99.2010905-at-comcast.net} 14, 19 -- Date: Wed, 25 Jan 2006 07:51:53 -0800 14, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 14, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 14, 19 -- X-Accept-Language: en-us, en 14, 19 -- MIME-Version: 1.0 14, 19 -- To: edelmare-at-muohio.edu, Microscopy List Server {microscopy-at-microscopy.com} 14, 19 -- Subject: Re: [Microscopy] Cryo-ultramicrotomy workshop? 14, 19 -- References: {200601242046.k0OKklJE029987-at-ns.microscopy.com} 14, 19 -- In-Reply-To: {200601242046.k0OKklJE029987-at-ns.microscopy.com} 14, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Link to current cryocourse June 6-15, 2006 at University of British Columbia, Vancouver, BC Here is the link to the current cryo course in 2006. http://www.emlab.ubc.ca/CryoEM2006/index.html
Judy Murphy wrote:
} The 5th International Cryo-EM Course is I believe June 6-17, 2006 in } Vancouver, Canada at University of British Columbia by Dr. Elaine } Humphrey from UBC and Dr. Kent McDonald, from UC, Berkeley with } international speakers. } This is an excellent course and caters to the needs of the particular } students. Be sure to check with Elaine about dates. } For more info email } Dr. Elaine Humphrey } ech-at-interchange.ubc.ca } } Information from the previous course is listed at } http://www.emlab.ubc.ca/CryoEM2005/index.html } } Judy } } } Judy Murphy, PhD } Microscopy Training, Imaging, and Lab Design } Stockton, CA 95219 } murphyjudy-at-comcast.net } } } } } } } } edelmare-at-muohio.edu wrote: } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } I have had a request from a user looking for a cryo- } } ultramicrotomy workshop. Is anyone offering one in the next year or } } know of one? Thanks! } } } } } } } } Richard E. Edelmann, Ph.D. } } Electron Microscopy Facility Director } } 364 Pearson Hall } } Miami University, Oxford, OH 45056 } } Ph: 513.529.5712 Fax: 513.529.4243 } } E-mail: edelmare-at-muohio.edu } } http://www.emf.muohio.edu } } } } "WE ARE MICROSOFT. } } RESISTANCE IS FUTILE. } } YOU WILL BE ASSIMILATED." } } } } ==============================Original } } Headers============================== } } 5, 23 -- From edelmare-at-muohio.edu Tue Jan 24 14:11:13 2006 } } 5, 23 -- Received: from mulnx12.mcs.muohio.edu } } (mulnx12.mcs.muohio.edu [134.53.6.67]) } } 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } k0OKBBkX020625 } } 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 } } 14:11:12 -0600 } } 5, 23 -- Received: from mulnx24.mcs.muohio.edu } } (mulnx24.mcs.muohio.edu [134.53.6.11]) } } 5, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) } } with ESMTP id k0OKB14j016596 } } 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 } } 15:11:01 -0500 } } 5, 23 -- Received: from emf03 ([134.53.14.97]) } } 5, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) } } with ESMTP id k0OKB002022369 } } 5, 23 -- for {microscopy-at-Microscopy.com} ; Tue, 24 Jan 2006 } } 15:11:00 -0500 } } 5, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} } } 5, 23 -- To: microscopy-at-Microscopy.com } } 5, 23 -- Date: Tue, 24 Jan 2006 15:11:49 -0500 } } 5, 23 -- MIME-Version: 1.0 } } 5, 23 -- Content-type: text/plain; charset=US-ASCII } } 5, 23 -- Content-transfer-encoding: 7BIT } } 5, 23 -- Subject: Cryo-ultramicrotomy workshop? } } 5, 23 -- Message-ID: {43D643B5.14764.643E209-at-localhost} } } 5, 23 -- Priority: normal } } 5, 23 -- X-mailer: Pegasus Mail for Win32 (v3.12c) } } 5, 23 -- X-Real-ConnectIP: 134.53.14.97 } } 5, 23 -- X-Scanned-By: MIMEDefang 2.45 } } 5, 23 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.11 } } ==============================End of - } } Headers============================== } } } } } } } }
==============================Original Headers============================== 5, 19 -- From murphyjudy-at-comcast.net Wed Jan 25 09:57:45 2006 5, 19 -- Received: from sccrmhc14.comcast.net (sccrmhc14.comcast.net [204.127.202.59]) 5, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PFviAM012953 5, 19 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 09:57:44 -0600 5, 19 -- Received: from [192.168.1.102] (c-67-181-78-13.hsd1.ca.comcast.net[67.181.78.13]) 5, 19 -- by comcast.net (sccrmhc14) with ESMTP 5, 19 -- id {2006012515574101400ejlo9e} ; Wed, 25 Jan 2006 15:57:42 +0000 5, 19 -- Message-ID: {43D79FFF.4030800-at-comcast.net} 5, 19 -- Date: Wed, 25 Jan 2006 07:57:51 -0800 5, 19 -- From: Judy Murphy {murphyjudy-at-comcast.net} 5, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 5, 19 -- X-Accept-Language: en-us, en 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy List Server {microscopy-at-microscopy.com} 5, 19 -- Subject: Re: [Microscopy] Cryo-ultramicrotomy workshop? 5, 19 -- References: {200601242046.k0OKklJE029987-at-ns.microscopy.com} {43D79E99.2010905-at-comcast.net} 5, 19 -- In-Reply-To: {43D79E99.2010905-at-comcast.net} 5, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am in need of a manual for the Olympus BX41 microscope, particularly the epifluorescence unit, U-L100HGAPO. If anybody knows of on-line instructions, or how I could obtain a copy of the manuals, I would be very grateful. I will be happy to pay any copying and mailing charges, of course.
Ralph Common Dept. of Physiology Michigan State University
==============================Original Headers============================== 4, 24 -- From rcommon-at-msu.edu Wed Jan 25 10:07:41 2006 4, 24 -- Received: from sys18.mail.msu.edu (sys18.mail.msu.edu [35.9.75.118]) 4, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0PG7dLa015524 4, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2006 10:07:39 -0600 4, 24 -- Received: from [35.9.122.125] (helo=emlab) 4, 24 -- by sys18.mail.msu.edu with esmtpsa (Exim 4.52 #1) 4, 24 -- (TLSv1:RC4-MD5:128) 4, 24 -- id 1F1nBK-0005iX-UD 4, 24 -- for Microscopy-at-microscopy.com; Wed, 25 Jan 2006 11:07:39 -0500 4, 24 -- From: "Ralph Common" {rcommon-at-msu.edu} 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- Subject: Olympus BX41 Manual 4, 24 -- Date: Wed, 25 Jan 2006 11:08:14 -0500 4, 24 -- Message-ID: {001201c621c9$8c9bed50$7d7a0923-at-msu.edu} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- charset="iso-8859-1" 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 (Normal) 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 4, 24 -- Importance: Normal 4, 24 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Hello Everyone, Is there anyone out there who has experience etching Cu and Ni sulfides, in order to see compositional zoning ?
The phases I am interested in are: Cu2S and Ni3-XS2. In both these compounds Ni and Cu substitute for each other. It is these substitutions which I suspect change the etching behaviour of the compounds. Etching could thus be a quick and dirty alternative to excessive X-ray mapping/analysis.
I don't have the answer, but you may want to post this question on Metallography.com for wider exposure.
Stu Smalinskas, P.E. Metallurgist SKF Plymouth, Michigan
--- shem-at-laurentian.ca wrote: } } Hello Everyone, } Is there anyone out there who has experience etching } Cu and Ni sulfides, in order } to see compositional zoning ? } } The phases I am interested in are: Cu2S and Ni3-XS2. } In both these compounds Ni and Cu substitute } for each other. It is these substitutions which I } suspect change the etching behaviour of the } compounds. } Etching could thus be a quick and dirty alternative } to excessive X-ray mapping/analysis. } } Any input is welcome ! } } Thanks, } } Skage Hem } } } } _______________________________________________________________ } } Skage Hem } Research Scientist, Ph.D. } CAF, Department of Earth Sciences } Laurentian University } Ramsey Lake Road } Sudbury, ON } Canada } P3E 2C6 } } ph. 705-675-1151 x4040 } cell. 705-562-7321 } fax 705-675-4898 } } http://laurentian.ca/geology/Research/hem.html } }
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 5, 20 -- From smalinskas-at-yahoo.com Wed Jan 25 15:39:12 2006 5, 20 -- Received: from web34105.mail.mud.yahoo.com (web34105.mail.mud.yahoo.com [66.163.178.103]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k0PLdBtD025507 5, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Wed, 25 Jan 2006 15:39:11 -0600 5, 20 -- Received: (qmail 12361 invoked by uid 60001); 25 Jan 2006 21:39:10 -0000 5, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 20 -- s=s1024; d=yahoo.com; 5, 20 -- h=Message-ID:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 20 -- b=1qmKDIUrud/YHnASQmqF6Vva92fi6hEC/jh1UNtRTvJH3OA4jU3Nb6FtrnQAiUR9lMgapzEfGIyQL+xNL6m1zL3xcbR+TJbBeW+komO12e06Smi5V2d6mx9x/L1/xO6qD4YjBOOzBjB4Ck7WEav53M6zgyoDWv+e+oZh+r9g318= ; 5, 20 -- Message-ID: {20060125213910.12359.qmail-at-web34105.mail.mud.yahoo.com} 5, 20 -- Received: from [141.151.33.213] by web34105.mail.mud.yahoo.com via HTTP; Wed, 25 Jan 2006 13:39:10 PST 5, 20 -- Date: Wed, 25 Jan 2006 13:39:10 -0800 (PST) 5, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 5, 20 -- Subject: Re: [Microscopy] Etching of Cu-Ni matte for reflected light microscopy 5, 20 -- To: shem-at-laurentian.ca, 5, 20 -- "microscopy-at-sparc5.microscopy.com" {microscopy-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200601252114.k0PLE5YS017833-at-ns.microscopy.com} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=iso-8859-1 5, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
THE TEXAS SOCIETY FOR MICROSCOPY invites you to our Spring 2006 meeting on April 20-22, 2006 at Alcon Research Labs, 6201 South Freeway, Ft. Worth, TX 76134
All registration forms and lodging details are available on our web site: http://www.texasmicroscopy.org/
ABSTRACTS MUST BE RECEIVED BY: March 20, 2006 Advanced Registration Deadline: April 7, 2006. Advanced registration is strongly suggested to afford TSM an accurate participant count for event organization.
**Workshops Thursday, April 20, 2006 (held at Alcon Labs, Ft. Worth)
Microwave Immunology 101 Sponsored by Ted Pella, Inc. Speaker: Rick Giberson, Sr. Applications Engineer
ESEM: not just for Biology Anymore Sponsored by FEI Company Speaker: Daniel Phifer, Sr. Applications Engineer
**Guest Speaker Friday, April 21, 2006
Materials Science in Museums Dr. Pamela Vandiver, Professor of Materials Science and Engineering and Archeology, Co-Director of Program in Heritage Conservation Science at the University of Arizona, and former Senior Research Scientist at the Smithsonian Institutions Center for Materials Research and Education.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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Invitation and Call for Papers Contamination Control in Electron and Ion Microscopy Microscopy and Microanalysis 2006, Chicago, IL Organizers: Ronald Vane (XEI Scientific) and Andrįs E. Vladįr, Ph.D. (NIST) Session A17 (POSTERS ONLY) ABSTRACTS DUE February 15, 2006
This session is being organized to present the latest research on contamination sources and control techniques in charged beam microscopy. Herein we invite papers on all aspects of contamination control inside electron microscopes or focused ion beam tools including sources of contamination, the effects of contamination, contamination artifacts and interference on microscopy results, and contamination control methods and techniques. Contamination includes hydrocarbons, particulates, and other foreign matter that may interfere with imaging and analysis.
Traditionally, contamination control in SEMs has focused on pump oils, fingerprints, dirty specimens, and good vacuum practice in manufacturing and service. The use of dry pumps at all stages of the vacuum system of new FESEMs (Field Emission SEMs) and the use of better vacuum practices on the part on users and manufacturers have reduced contamination, but not eliminated problems. Most commonly, tools in the field begin to exhibit contamination artifacts after several months of use due to trace amounts of contaminants brought in with specimens. The Semiconductor and nano-sciences industries have demanded tools that can image structures {2 nm in size at { 2kV. Instrument manufacturers have responded with Field Emission tools that easily produce better than 400 kX magnification at high contrast with low kV beams. Control of contamination has assumed greater significance as semiconductor companies move to ever smaller dimensions. It is now common to observe features with {2 kV and {10 nm in size, close to these tools resolution limits. In such cases, the slightest amount of hydrocarbon (HC) contamination in the chamber can cause loss of resolution and contrast. The electron beam reacts with any stray HC in the beam path or on the surface creating HC ions that condense and form hydrocarbon deposits on the area being scanned. Even with baking, dry pumps and LN traps, artifacts and contamination haze remains.
What are the sources and what can be done? Some Research Topic Ideas:
* Do Hydrocarbons adsorbed on materials migrate on the surface to interact with charged beams to form deposits or is vapor phase transport more important? The effectiveness of cold fingers and Evactron(R) chamber cleaning suggests that vapor phase transport of contaminants is an important alternative mechanism to surface transport.
* Is the gas phase interaction of the electron beam and contaminant molecules important in SEM contamination buildup? Electron impact ionization is a common technique in mass spectrometry of organics. What is the importance of electron impact ionization on vapor phase organics in the SEM in causing contamination build up in scanned areas?
* What gives better results: plasma cleaning every specimen before introduction to the SEM chamber, Evactron chamber cleaning, or Evactron cleaning specimens in the chamber?
* Study Hydrocarbon removal rates for different decontamination methods, for different types of hydrocarbons and concentrations, under different Evactron De-Contaminator power and pressure settings, and for different pump speeds and flow rates during Evactron cleaning.
* How fast does AMC (atmospheric molecular contamination) accumulate on specimens in different environments after they are cleaned? Freshly prepared and cleaned specimens are known to be freer from contamination than specimens that have sat in room air for several days. Quantify this effect.
* Establish a standard procedure for depositing repeatable amounts of contamination for removal technique studies.
* Establish a protocol for measuring tool contamination that can be transferred between tools and used to compare tools or used to establish contamination standards or limits.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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Email: kjl226-at-vt.edu Name: Kathy
Organization: Virginia Tech
Title-Subject: [Filtered] Embedding spores
Question: What is the best way to process and embed spores?
Why not just ask Olympus, I'm pretty sure they will have one as its still a current microscope. If you are happy with a photocopy expect to get it free of charge from your local rep (and I'm sure they will email you the pdf version as well).
Zeiss and Leica now have all the recent microscope manuals on-line, but I have obtained photocopied manuals for 20+ year old microscopes and fittings, and once even a BASIC program hardware/software guide for a very odd programmable HP+Zeiss motorised XY stage discontinued in the 1970's (in the 1990's). The most interesting bit was the BASIC 'Trace on' debugging command: 'TRON', but to be honest it worked better when Disney did it.
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
The session organizers for the Pharmaceutical Symposium of M&M 2006 are looking for additional speakers. Presentations should discuss biological or material science applications of significance to the pharmaceutical industry. The meeting will be held in Chicago, July 30 - Aug. 3. The abstract submission deadline is Feb. 15, 2006. If you are interested in submitting a platform or poster presentation, instructions for abstract submission are available at the meeting web site at http://mm2006.microscopy.org/ . For additional questions please contact the session chairs at the addresses below. Thanks.
Joe Neilly Abbott Laboratories 200 Abbott Park Rd. Bldg. AP31, Dept. R4R9 Abbott Park, IL 60064-6202 email: joe.neilly-at-abbott.com voice: 847-938-5024
Jim DiOreo Baxter Healthcare RL WG3-2S Route 120 and Wilson Road Round Lake, IL 60002 email: jim_dioreo-at-baxter.com voice: 847-270-4676
==============================Original Headers============================== 3, 18 -- From joe.p.neilly-at-abbott.com Thu Jan 26 07:59:45 2006 3, 18 -- Received: from abtmx5.abbott.com (abtmx5.abbott.com [130.36.44.95]) 3, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QDxiEW028526 3, 18 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 07:59:45 -0600 3, 18 -- Received: from abtapn561.northamerica.intra.abbott.com (abtapn561.northamerica.intra.abbott.com [10.236.188.103]) 3, 18 -- by abtmx5.abbott.com (Switch-3.1.7/Switch-3.1.7) with ESMTP id k0QDxeU7010209 3, 18 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 07:59:40 -0600 (CST) 3, 18 -- To: microscopy-at-microscopy.com 3, 18 -- Subject: Request for Speakers at Pharmaceutical Symposium , M&M 2006 3, 18 -- X-Mailer: Lotus Notes Release 5.0.5 September 22, 2000 3, 18 -- Message-ID: {OFE0F856E6.F3ED62DE-ON86257102.004CC6F7-at-abbott.com} 3, 18 -- From: Joseph P Neilly {joe.p.neilly-at-abbott.com} 3, 18 -- Date: Thu, 26 Jan 2006 07:59:12 -0600 3, 18 -- X-MIMETrack: Serialize by Router on ABTAPN561/ESVR/ABBOTT(Release 6.5.4FP2|September 12, 2005) at 3, 18 -- 01/26/2006 07:59:42, 3, 18 -- Serialize complete at 01/26/2006 07:59:42 3, 18 -- MIME-Version: 1.0 3, 18 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
We have a networked color laser printer in our lab which we use to print routine image / working print from our SEM's and otehr microscopes. We print images and sectra, EBDS, etc. What we have found is that fewer and fewer folks actually want hardcopies. They want and use electronic versions, since even for publication nearly all of the journals these days want electronic only. My printer supplies are about 20% or lower of what they were 2-3 years ago (i.e. over an 80% decrease in printing - at the same time I've seen a 28% increase in user numbers).
For high quality and archive prints we use our Epson 9600 wide format printer - we generally use if for posters. I would not hesitate to get another Epson Pro series, perhaps in a smaller format for more routine size prints. Or look at the HP highend printers.
But remember the inks will dry in the printers if they are not regularly used. Some of the manufacturers have inks which are designed to last longer in the printer - but they are not the low- to medium end printers. Dry inks like laser printers and wax/polymer "phaser" printers have an advantage here.
FYI: We have a Lexmark C752 color laser printer. And whereas we've been very very happy with the durablity and quality of the Lexmark Black / Greyscale laser printers, we have not been thrilled with the image quality of the C752 prints, and yes we use the high quality color laser paper.
On 25 Jan 2006, at 7:44, fmalherbe-at-swin.edu.au wrote:
} } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Below is the result of your feedback form (NJZFM-ultra-55). It was } submitted by (fmalherbe-at-swin.edu.au) from } http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html } on Tuesday, January 24, 2006 at 18:23:45 } ---------------------------------------------------------------------- } ----- } } Email: fmalherbe-at-swin.edu.au } Name: Francois Malherbe } } Organization: Swinburne University of Technology } } Education: Graduate College } } Location: Melbourne, Victoria, Australia } } Question: We are purchasing a new SEM, what are the basic specs you } would recommend for the printer: } } - inkjet, laser or other? } - USB, serial? } - dpi? } } Thank you for your help. } } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== 9, 12 -- From } zaluzec-at-ultra5.microscopy.com Wed Jan 25 07:33:17 2006 9, 12 -- } Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, } 12 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k0PDXFin024204 9, 12 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan } 2006 07:33:16 -0600 9, 12 -- Mime-Version: 1.0 9, 12 -- X-Sender: } zaluzec-at-ultra5.microscopy.com (Unverified) 9, 12 -- Message-Id: } {p06110405bffd2e8756cc-at-[206.69.208.22]} 9, 12 -- Date: Wed, 25 Jan } 2006 07:33:15 -0600 9, 12 -- To: microscopy-at-microscopy.com 9, 12 -- } From: fmalherbe-at-swin.edu.au (by way of Ask-A-Microscopist) 9, 12 -- } Subject: [Filtered] AskAMicroscopist: Printer for an SEM 9, 12 -- } Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
"WE ARE MICROSOFT. RESISTANCE IS FUTILE. YOU WILL BE ASSIMILATED."
==============================Original Headers============================== 12, 25 -- From edelmare-at-muohio.edu Thu Jan 26 09:13:08 2006 12, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 12, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QFD7wO013454 12, 25 -- for {microscopy-at-Microscopy.com} ; Thu, 26 Jan 2006 09:13:07 -0600 12, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 12, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0QFD5ac030704; 12, 25 -- Thu, 26 Jan 2006 10:13:05 -0500 12, 25 -- Received: from emf03 ([134.53.14.97]) 12, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k0QFD4fg029547; 12, 25 -- Thu, 26 Jan 2006 10:13:04 -0500 12, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 25 -- To: fmalherbe-at-swin.edu.au 12, 25 -- Date: Thu, 26 Jan 2006 10:13:59 -0500 12, 25 -- MIME-Version: 1.0 12, 25 -- Content-type: text/plain; charset=US-ASCII 12, 25 -- Content-transfer-encoding: 7BIT 12, 25 -- Subject: Re: [Microscopy] [Filtered] AskAMicroscopist: Printer for an SEM 12, 25 -- CC: microscopy-at-Microscopy.com 12, 25 -- Message-ID: {43D8A0E7.2878.F7FC50B-at-localhost} 12, 25 -- Priority: normal 12, 25 -- In-reply-to: {200601251344.k0PDiMAx027101-at-ns.microscopy.com} 12, 25 -- X-mailer: Pegasus Mail for Win32 (v3.12c) 12, 25 -- X-Real-ConnectIP: 134.53.14.97 12, 25 -- X-Scanned-By: MIMEDefang 2.45 12, 25 -- X-Scanned-By: MIMEDefang 2.52 on 134.53.6.10 ==============================End of - Headers==============================
Do you mean using glass plates instead of film? If so ..... Plates are much thicker and heavier, these factors contribute to storage problems. Plates break when dropped, film does not. I know people who insist that plates give a better image but I have seen no evidence of that. The boxes that Kodak plates came in were great for storing lantern slides.
Geoff
Ken.Blight-at-cancer.org.uk wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 32 -- From mcauliff-at-umdnj.edu Thu Jan 26 09:19:07 2006 8, 32 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 8, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QFJ71O014621 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Thu, 26 Jan 2006 09:19:07 -0600 8, 32 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 8, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 263D14BE18 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Thu, 26 Jan 2006 10:19:07 -0500 (EST) 8, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 8, 32 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 785834BDF9 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Thu, 26 Jan 2006 10:19:06 -0500 (EST) 8, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 8, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 8, 32 -- id {0ITP00L01GB01W-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 8, 32 -- for microscopy-at-msa.microscopy.com; Thu, 26 Jan 2006 10:19:06 -0500 (EST) 8, 32 -- Received: from [127.0.0.1] ([10.138.2.240]) 8, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 8, 32 -- 2004)) with ESMTP id {0ITP00JRVGQKBF-at-Polaris.umdnj.edu} ; Thu, 8, 32 -- 26 Jan 2006 10:08:58 -0500 (EST) 8, 32 -- Date: Thu, 26 Jan 2006 10:08:10 -0500 8, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 32 -- Subject: Re: plates 8, 32 -- In-reply-to: {200601261358.k0QDwKjM028252-at-ns.microscopy.com} 8, 32 -- To: Ken.Blight-at-cancer.org.uk, 8, 32 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 8, 32 -- Message-id: {43D8E5DA.6030702-at-umdnj.edu} 8, 32 -- MIME-version: 1.0 8, 32 -- Content-type: text/plain; format=flowed; charset=us-ascii 8, 32 -- Content-transfer-encoding: 7BIT 8, 32 -- X-Accept-Language: en-us, en 8, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 8, 32 -- Gecko/20040804 Netscape/7.2 (ax) 8, 32 -- References: {200601261358.k0QDwKjM028252-at-ns.microscopy.com} ==============================End of - Headers==============================
Sorry for the confusion caused -The imaging plates I am interested in are electron detection plates for high quality digital images. I have no other details otherwise I would not be asking the question!
ken
Ken Blight Senior Scientific Officer Electron Microscopy Cancer Research UK London England.
==============================Original Headers============================== 4, 14 -- From Ken.Blight-at-cancer.org.uk Thu Jan 26 09:29:15 2006 4, 14 -- Received: from magic.lif.icnet.uk (magic.lif.icnet.uk [143.65.100.6]) 4, 14 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0QFTEVw017335 4, 14 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 09:29:14 -0600 4, 14 -- Received: from [143.65.59.12] ([143.65.59.12]) 4, 14 -- by magic.lif.icnet.uk (8.9.3p2/8.9.3) with ESMTP id PAA27526 4, 14 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2006 15:29:13 GMT 4, 14 -- Mime-Version: 1.0 4, 14 -- Message-Id: {a06200702bffe98674b18-at-[143.65.59.12]} 4, 14 -- Date: Thu, 26 Jan 2006 15:25:01 +0000 4, 14 -- To: microscopy-at-microscopy.com 4, 14 -- From: Ken Blight {Ken.Blight-at-cancer.org.uk} 4, 14 -- Subject: Imaging plates 4, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
What you want is described at http://www.berthold.com.au/imaging_pages/FDL-5000.html
and at http://www.ditabis.de/
I have no experience with use of these in EM. An impressive 3D cryo-EM paper based on use of the imaging plates was published by Holmes et al in Nature (2003), 425:423-427. Use of imaging plates requires drying the media before loading into the camera, and requires breaking camera vacuum and accessing the camera in order to remove media to "develop" (scan & digitize) the recorded images and regenerate the plate. Both steps are avoided when using CCD display and recording of EM images, which provide the other route for directly digitizing EM images without processing and scanning photographic negatives.
-mike reedy-
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-- -mike reedy-
************************ Michael K. Reedy, M.D. Duke Univ. Med. Center Dept. Cell Biology, Box 3011 (for U.S. Mail) 458 Alex Sands Bldg, Research Dr (courier) Durham, NC 27710
I hope you all will forgive this detour from microscopy. If you have a x-ray film processor in your lab and would care to comment on X-ray film processors I would love to hear from you off-list. We have an AFP Medical-Mini X-ray film processing system but we are looking into replacing it with a Konica SRX-101A desktop processor. Advice/ opinions on this equipment would be greatly appreciated. Thanks, Beth
********************************************************************** Beth Richardson EM Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
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Title-Subject: [Filtered] TEM - Seeking to acquire JEOL jem100c or equivalent.
Question: We are a small French company based in the Paris region, currently looking to replace our JEOL jem100c transmission electron microscope, which unfortunately, has broken down.
We need to acquire a second-hand microscope similar to the model we actually have but as we wish to use it mainly as an electron generator, to test the performance of the customized cameras we manufacture, we would accept a tem microscope, which does not offer all the requirements necessary for good quality imagery.
If you have such equipment and are willing to give us the opportunity of acquiring it, please contact us via our e-mail address: info-at-lheritier-sa.com.
It looks like we are losing the +24V section of the power supply on our Oxford ISIS EDS. Does anyone have a spare power supply, or even an entire ISIS unit they would be willing to part with? It probably should be a model 200 or 300.
Please contact me off-line and we can discuss the details.
Warren Straszheim Materials Analysis and Research Lab Iowa State University 515-294-8187
==============================Original Headers============================== 4, 29 -- From wesaia-at-iastate.edu Fri Jan 27 13:37:34 2006 4, 29 -- Received: from mailhub-5.iastate.edu (mailhub-5.iastate.edu [129.186.140.15]) 4, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0RJbXOC001093 4, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Jan 2006 13:37:34 -0600 4, 29 -- Received: from mailout-2.iastate.edu (mailout-2.iastate.edu [129.186.140.2]) 4, 29 -- by mailhub-5.iastate.edu (8.12.11/8.12.10) with SMTP id k0RJbWHI009451 4, 29 -- for {Microscopy-at-Microscopy.Com} ; Fri, 27 Jan 2006 13:37:32 -0600 4, 29 -- Received: from owa.eng.iastate.edu(129.186.23.85) by mailout-2.iastate.edu via smtp 4, 29 -- id 7bca_5f49d450_8f6c_11da_9c60_003048290bef; 4, 29 -- Fri, 27 Jan 2006 13:37:37 -0600 4, 29 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.1830); 4, 29 -- Fri, 27 Jan 2006 13:37:32 -0600 4, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 4, 29 -- Content-class: urn:content-classes:message 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; 4, 29 -- charset="us-ascii" 4, 29 -- Subject: Need ISIS power supply 4, 29 -- Date: Fri, 27 Jan 2006 13:38:47 -0600 4, 29 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7275F1FFC-at-maire.eng.iastate.edu} 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- Thread-Topic: Need ISIS power supply 4, 29 -- Thread-Index: AcYjeUgRcQ/G34hJQ8agDRbdnqwxXA== 4, 29 -- From: "Straszheim, Warren E [CCE E]" {wesaia-at-iastate.edu} 4, 29 -- To: {Microscopy-at-Microscopy.Com} 4, 29 -- X-OriginalArrivalTime: 27 Jan 2006 19:37:33.0133 (UTC) FILETIME=[1E3347D0:01C62379] 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0RJbXOC001093 ==============================End of - Headers==============================
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Question: Title: Advanced Techniques in Microscopy for Materials Characterization
Lecturers: David Joy, University of Tennessee Eric Lifshin, State University of New York George Vander Voort, Buehler Ltd. Raynald Gauvin, McGill University Brendan Griffin, University of Western Australia Rocco Cerchiara, Fischione Instruments Pierre Hovington, Hydro-Quebec Research Institute Tom Kelly, Imago Scientific Instruments Scott Sitzman, HKL Technology Marin Lagace, Hydro-Quebec Research Institute
When: May 8-12, 2006
Where: McGill University, Department of Mining, Metals and Materials Engineering M.H.Wong Building 3610 University Street Montreal, Quebec, Canada H3A 2B2
The worlds largest and most comprehensive microscopy and microanalysis meeting is coming to Chicago July 30-Aug 3, 2006. This is the perfect opportunity for both your and as well as undergraduate/graduate students to gain valuable insights to recent work by interacting with the worlds most prominent researchers, as well as learn about the latest techniques in the scientific symposia and in instrumentation.
In addition to more than 50 scientific sessions and symposia there will be a number of short courses/tutorials starting on the weekend and extending through the week.
The meeting also hosts the largest commerical exhibition of microscopy instrumentation at any venue.
This will be the perfect opportunity for you to take advantage of the premier international meeting happening in the USA as well as make valuable contacts for the future.
Of particular importance to students is the availability of scholarship, and other funding opportunities for students which will allow you to attend the meeting at reduced costs.
*Student receive reduced registration rates * Scholarships and Awards are available from the sponsoring societies * Poster awards are presented for the best student contributions * Student Bursaries (i.e. work part time during the meeting) can be used to help defray your expenses
A limited number of awards are also available for professional technical staff.
To be eligible for these opportunities you must submit a paper for either platform or poster presentation at the meeting. The deadline for submitting a paper to MM2006 is Feb. 15, 2006 (there are no extensions), which is only a few weeks away.
If you have any question please feel free to contact any of the Organizers, for detailed information please visit the meeting WWW site (http://mm2006.microscopy.org).
Again the paper submission deadline is Feb 15, 2006. I will also point out that the submission process is now entirely electronic so you still have plenty of time to prepare your work and submit it for review and consideration. All papers for accepted for the meeting are included in the proceedings which is published as supplement to the Journal of the Microscopy Society of America - Microscopy & Microanalysis.
Disclaimer: In additions to being your Friendly Neighborhood SysOp, I have the "honor" (cough) of being the Co-chair of the local arrangements committee for this meeting, thus I have a vested interest in getting you to come to Chicago!
Cheers...
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 16, 11 -- From zaluzec-at-microscopy.com Mon Jan 30 08:31:29 2006 16, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 16, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0UEVSk1010268 16, 11 -- for {microscopy-at-microscopy.com} ; Mon, 30 Jan 2006 08:31:29 -0600 16, 11 -- Mime-Version: 1.0 16, 11 -- Message-Id: {p06110401c003ccb17092-at-[206.69.208.22]} 16, 11 -- Date: Mon, 30 Jan 2006 08:31:28 -0600 16, 11 -- To: microscopy-at-microscopy.com 16, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 16, 11 -- Subject: MM 2006 Paper Submission Deadline - Feb 15, 2006 16, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Hi Everyone I am looking for a EHT X-regulator for our old Phillips EM 300 TEM. Anybody knows a good place to buy this part or have such parts on sale please contact me at pjoshi-at-miami.edu Thank you
Pratik P. Joshi, Ph.D.
Assistant Scientist / Lab Manager Center for Advanced Microscopy Department of Chemistry, University of Miami 1301 Memorial Drive, Room 315 Miami, FL- 33146 Ph: (305)284-4736, Fax: (305)284-1880
==============================Original Headers============================== 2, 20 -- From pjoshi-at-miami.edu Mon Jan 30 08:58:40 2006 2, 20 -- Received: from EXCHANGE2.cgcent.miami.edu (ntexchsrv2.ir.miami.edu [129.171.32.59]) 2, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0UEwd88014815 2, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2006 08:58:39 -0600 2, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 20 -- Content-class: urn:content-classes:message 2, 20 -- MIME-Version: 1.0 2, 20 -- Content-Type: text/plain; 2, 20 -- charset="iso-8859-1" 2, 20 -- Subject: Re: Looking for EHT X Regulator unit for Phillips EM 300 TEM 2, 20 -- Date: Mon, 30 Jan 2006 09:58:34 -0500 2, 20 -- Message-ID: {B2BA593DA3EBE143A856ABA856D01F5D02DCFE55-at-EXCHANGE2.cgcent.miami.edu} 2, 20 -- X-MS-Has-Attach: 2, 20 -- X-MS-TNEF-Correlator: 2, 20 -- Thread-Topic: Looking for EHT X Regulator unit for Phillips EM 300 TEM 2, 20 -- Thread-Index: AcYlraSDK/Vfxr9oTDWLGaysH9W/UA== 2, 20 -- From: "Joshi, Pratik P" {pjoshi-at-miami.edu} 2, 20 -- To: {Microscopy-at-microscopy.com} 2, 20 -- Content-Transfer-Encoding: 8bit 2, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0UEwd88014815 ==============================End of - Headers==============================
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Email: brad.huggins-at-bp.com Name: Brad Huggins
Organization: BP Chemicals, Naperville, IL
Title-Subject: [Filtered] LM, Anyone with "Digital Microscope" familiarity/experience?
Question: (trouble with MS Outlook and spam errors, so I'm using this format)
For several remote, light microscope applications, I have been looking at the Keyence VHX-100, Digital Microscope. It looks to be a very capable and versatile tool. Aside from its portability, 3D imaging capability appears quite impressive to me. It looks capable of adding - very significantly - to the limited depth-of-focus imaging condition of our existing light microscope systems.
Does anyone on the ListServer have any experience (good, bad or otherwise) with this microscope? Or similar digital microscopes? If you would rather not respond online, please share with me off-line. I will share a summary of "anonymous responses" as appropriate.
Thanks in advance,
Brad Huggins
BP Chemicals Naperville, IL brad.huggins-at-bp.com 630 420-3668
I would like hear the comment on various sample preparation technique for confocal microscopy (indirect Immunofluorescence) which gives 1) best presarvation 2) minimum background noise
Is it advisible to dry the slide before switching over to 1 Ab and 2Ab. I mean to ask is this drying in between lead to some form of precipitate???
thanks in advance
Regards, Shrunali Kulkarni Scientist Institute of Microbial Technology India
__________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com
==============================Original Headers============================== 6, 18 -- From aarti_harle-at-yahoo.co.in Tue Jan 31 22:35:20 2006 6, 18 -- Received: from web8303.mail.in.yahoo.com (web8303.mail.in.yahoo.com [202.43.219.124]) 6, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k114ZGAT025969 6, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Jan 2006 22:35:17 -0600 6, 18 -- Received: (qmail 86863 invoked by uid 60001); 1 Feb 2006 04:35:18 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.co.in; 6, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 18 -- b=AkdqGUkec0jNq9kRo7Ys1VDUDL9lmWiPYTrbZimG2x1gKtvKw56/ZRooXkIC5egeuXa/ocAJv2H7KBo8JPcd2YKl7to7s51Bgw1hKsKk03kglfqBuZIch4AbZnTxASV5UcSCJ1oiDR9TfNDpAWfoQmdp+Ov+/Y2ZET4xGUPfwjE= ; 6, 18 -- Message-ID: {20060201043518.86861.qmail-at-web8303.mail.in.yahoo.com} 6, 18 -- Received: from [203.197.210.215] by web8303.mail.in.yahoo.com via HTTP; Tue, 31 Jan 2006 20:35:18 PST 6, 18 -- Date: Tue, 31 Jan 2006 20:35:18 -0800 (PST) 6, 18 -- From: Aarti Harle {aarti_harle-at-yahoo.co.in} 6, 18 -- Subject: Sample preparation technique for confocal microscopy 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=iso-8859-1 6, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Yes it works fine (as do many anti-fadents e.g Citifluor http://www.citifluor.co.uk ), although we rarely get problems with DAPI bleaching - I won't mention Hoescht as I can never spell it. Normally the likes of Cy5 or TRITC type fluorochromes bleach first, and anyway DAPI is normally only there in our case to identify the animal cells more easily (and it really makes specimen focussing a cinch with our 'low contrast' samples). Vectashield antifadents stop samples fading really quickly rather than eliminating fading. So you still have to minimise laser (and Hg lamp) exposure times (e.g. increase scan speed, reduce image size & image averaging, increase gain, and, normally in the last resort, open up the confocal iris), particularly if you are doing Z stacks (naturally time-lapse won't be a problem with fixed 'Vectashield' samples).
Being Cell Biology, most of our specimens are derived from cell cultures and so have little contrast, making cell visual separation and location more difficult even with DIC. We only have a little 5 Mw 'violet' 405nm laser with our Leica SP2 AOBS confocal, but it works very well with nuclear stains despite being at the very edge of the DAPI/Hoescht excitation spectrum. We mostly use Mattek dishes (Petri dishes with a hole at the base and little cover slips glued on - see http://www.glass-bottom-dishes.com/ ) as all our microscopes are inverted and we only use high power oil objectives. They have the advantage that we can simply drip the Vectashield into the Petri dish on top of the fixed cells and replenish if it dries out (samples often keep for days or longer in a darkened fridge). With glass slides, the coverslip needs sealing on over the Vectashield with nail varnish, but the nail varnish (and marker pen ink) often dissolve into the immersion oil making a bit of a mess, particularly with the inverted microscopes we use. The nail varnish also sticks to the stage slide holder (as the slide has to be placed upside down in inverted systems) making a sticky mess and knocking the sample out of level.
With regard to preparation, we don't deal with microbiological specimens, just mammalian cells - so I won't offer any advise. There are plenty of recipes on the internet and in books though, and you can try contacting staff at another microbiological institutes via on-line searches or traditional 'old boy' networks. This list server tends to be biased a little towards electron microscopy. You reduce confocal image 'noise' in samples by lowering the scan speed, increasing image averaging and upping laser power on the confocal - the exact opposite of that required to minimise beaching. So there is always a compromise between image quality and fluorochrome bleaching. If the sample preparation has worked well your sample would be expected to be quite bright initially (prior to bleaching) - often problems with dark images are due to experimental failure rather than the confocal microscope and it's software.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {aarti_harle-at-yahoo.co.in} To: {keith.morris-at-ucl.ac.uk} Sent: Tuesday, January 31, 2006 1:02 PM
Dear Shrunali,
I forgot to add it (although I expect you have the link already) : The full details on Vectorshield products [e.g. Hardset and Mounting Medium versions] can be found on the manufacturers website :
It naturally has the 'test on an inconspicuous area before using' disclaimer. Anti-fadents have been reported to occasionally cause a leaching of stain and loss of fluorescence in some instances.
Regards
Keith
---------------------------------------------------------- Dr Keith J Morris Imaging Facilities Manager Cell Biology Division Institute of Ophthalmology University College London 11-43 Bath Street London EC1V 9EL
----- Original Message ----- X-from: {aarti_harle-at-yahoo.co.in} To: {keith.morris-at-ucl.ac.uk} Sent: Tuesday, January 31, 2006 1:02 PM
I am interested in hearing what type of gases people are using in their ESEM applications. We recently learned our silicon drift detector is incompatible with water vapor, and we apparently cannot complete any x-ray microanalysis while working in standard ESEM or VP modes (I was pretty surprised to learn this). We are thinking that using a dry gas such as nitrogen or helium will allow us to work in ESEM modes and use our x-ray system as well, as there is no potential for moisture condensing on the crystal surface.
Our ESEM is a Quanta 200, and it is not equipped with the peltier stage, so we mainly use the environmental modes to alleviate charging in uncoated samples. The x-ray system was purchased with the microscope about four years ago. I would rather not discuss the name of the x-ray vendor on the list, as we have a significant investment in this detector and do not foresee finding the money to purchase a new one soon.
Our first concern is the impact of the gas on spectra. We can coat samples and run them in high vacuum mode, but customers like spectra that represent the sample and not the coating material. We periodically have samples that cannot be coated, so ESEM becomes critical for our imaging needs. If the gas is going to have a dramatic impact on signal, are we better off coating and running high vacuum?
Is there a best choice for chamber gas? Our service engineer recommends nitrogen as having benefits for keeping the system clean. We can set it up fairly quickly, and I have a spare tank and regulator. I have heard of people using helium and believe that I read somewhere that there was some advantage to using helium.
I am still trying to get information from the vendor on whether this will allow us to use the x-ray and ESEM simultaneously, or whether we have options with different collimators or windows. Their responses have been pretty slow coming.
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238
==============================Original Headers============================== 9, 23 -- From hagglundk1-at-nku.edu Wed Feb 1 08:09:02 2006 9, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11E92h6029071 9, 23 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 08:09:02 -0600 9, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 9, 23 -- Wed, 1 Feb 2006 09:09:02 -0500 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="us-ascii" 9, 23 -- Subject: ESEM chamber gas choices 9, 23 -- Date: Wed, 1 Feb 2006 09:09:01 -0500 9, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F35861A-at-mailfac1.hh.nku.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: ESEM chamber gas choices 9, 23 -- Thread-Index: AcYnOQywNZQdWqyLTzaT/PcS/myvmA== 9, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 01 Feb 2006 14:09:02.0302 (UTC) FILETIME=[0DB1E7E0:01C62739] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k11E92h6029071 ==============================End of - Headers==============================
I am looking for information about staffing, equipment and general practices in microscopy facilities at other institutions. If anyone else is interested in the same sort of information, please reply to me directly. Thank you.
Lesley Bechtold
Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322
==============================Original Headers============================== 7, 21 -- From lesley.bechtold-at-jax.org Wed Feb 1 09:23:28 2006 7, 21 -- Received: from carmen.jax.org (carmen.jax.org [192.43.249.16]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11FNO0c004460 7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 09:23:26 -0600 7, 21 -- Received: from jcs-mid-prod.jax.org (jcs-mid-prod.jax.org [192.43.249.134]) 7, 21 -- by carmen.jax.org (8.12.11/8.12.11) with ESMTP id k11FLEmh001364 7, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 10:23:14 -0500 (EST) 7, 21 -- (envelope-from lesley.bechtold-at-jax.org) 7, 21 -- Received: from spikey.jax.org by jcs-mid-prod.jax.org 7, 21 -- with ESMTP id 65124051138807281; Wed, 01 Feb 2006 10:21:21 -0500 7, 21 -- From: "Lesley Bechtold" {lesley.bechtold-at-jax.org} 7, 21 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 21 -- Subject: Seeking information 7, 21 -- Date: Wed, 1 Feb 2006 10:20:25 -0500 7, 21 -- Message-ID: {20060201102025314.00000001972-at-spikey} 7, 21 -- X-Mailer: Oracle Connector for Outlook 10.1.1.0.5 71011 (11.0.6568) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; charset=us-ascii 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k11FNO0c004460 ==============================End of - Headers==============================
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Email: ptomic-at-ciclonsemi.com Name: Peter Tomic
Organization: Ciclon Semiconductor Device Corp.
Title-Subject: [Filtered] ELYMA
Question: I would like to hear from people in the semiconductor industry on their experience with ELYMAT [Electrolytical Metal Analysis Tool]. This is an imaging system for the analysis of metal and oxygen contamination in Si wafer technology.
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Email: twigg-at-estd.nrl.navy.mil Name: Mark Twigg
Organization: Naval Research Laboratory
Title-Subject: [Filtered] Postdoctural Position
Question: Postdoctoral Research Position Electronics Science and Technology Division Naval Research Laboratory, Washington, DC
We are seeking a Postdoctoral fellow with a strong transmission electron microscopy and extended-defects/nanostructures background to join our electronic materials program at NRL. You will utilize your skills to characterize SiC and GaN epitaxial layers, as well as nanoelectronic materials based on colloidal gold. We have our own Hitachi H9000UHR HRTEM and sample preparation laboratory. We also have access to a Philips CM30, a JEOL 2200 FETEM with Omega energy filter and Z-contrast STEM, and an FEI Nova 600 Dual Beam FIB. The qualified candidate should have a Ph.D. in Materials Science, Physics, Chemistry, or a related physical science or engineering discipline. Experience in a variety of electron microscopy techniques including HRTEM, CBED, and EDXS, and EELS is important. Experience with FIB, XTEM, and mechanical lapping sample preparation techniques is also desirable. Participants must be citizens or permanent residents of the United States. Two postdoctoral study programs are available at the Naval Research Laboratory: NRC and ASEE. NRC postdoctoral positions are administered by the National Research Council, a division of the National Academy of Sciences. The ASEE Postdoctoral Fellowship Program is administered by the American Society for Engineering Education. Further information about NRL's NRC post-doc program is found on the NRC web site (http://hroffice.nrl.navy.mil/jobs/postdoc.htm). Fellowships are awarded for one year and may be extended for a second year. The base annual stipend for both programs the first year is $62,886.
Point of Contact: Dr. Mark E. Twigg Naval Research Laboratory Code 6812 4555 Overlook Avenue, S.W. Washington, DC 20375-5320
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Has anyone used this buffer before? I plan on using this to make up a fixative to perfuse fix a rat. I will be looking at myelin of the sciatic nerve. Why does this buffer have such a high osmolarity? Any thoughts?
Chip, I've never used Tyrode's in conjunction with cacodylate. Years ago, when the lab I was in was collaborating with an electrophysiologist looking at structure-function relationships in heart muscle, we used Tyrode's because it is a bicarbonate-buffered solution and he could keep the isolated papillary muscles happy in it for hours as long as he bubbled oxygen-CO2 through it. Lovely structure (see various papers by Robinson, TR, etal during the 1980's). Lee -- Leona Cohen-Gould, M.S. Sr. Staff Associate Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Joan & Sanford I. Weill Medical College of Cornell University voice (212)746-6146 fax (212)746-8175 http://www.cornellcelldevbiology.org
==============================Original Headers============================== 1, 21 -- From lcgould-at-med.cornell.edu Wed Feb 1 12:54:15 2006 1, 21 -- Received: from smtp-gw2.med.cornell.edu (smtp-gw2.med.cornell.edu [157.139.3.45]) 1, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11IsEXQ002540 1, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 12:54:15 -0600 1, 21 -- Received: from mpx1.med.cornell.edu (biglb-vlan511vip.med.cornell.edu [140.251.11.120]) 1, 21 -- by smtp-gw2.med.cornell.edu (Switch-3.1.7/Switch-3.1.7) with ESMTP id k11IsBig011013 1, 21 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 13:54:11 -0500 (EST) 1, 21 -- Received: from [140.251.48.23] by mpx1.med.cornell.edu 1, 21 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 2005)) 1, 21 -- with ESMTP id {0IU0000UJV6ABKC0-at-mpx1.med.cornell.edu} for 1, 21 -- microscopy-at-microscopy.com; Wed, 01 Feb 2006 13:54:11 -0500 (EST) 1, 21 -- Date: Wed, 01 Feb 2006 13:50:59 -0500 1, 21 -- From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} 1, 21 -- Subject: Re: [Microscopy] viaWWW: Tyrodes-Cacodylate Buffer 1, 21 -- In-reply-to: {200602011727.k11HRkBD005210-at-ns.microscopy.com} 1, 21 -- To: dyel-at-mail.nih.gov, microscopy-at-microscopy.com 1, 21 -- Message-id: {p06200705c006b2a8a260-at-[140.251.48.23]} 1, 21 -- MIME-version: 1.0 1, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 1, 21 -- References: {200602011727.k11HRkBD005210-at-ns.microscopy.com} 1, 21 -- X-PMX-Version: 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2006.02.01.100606 ==============================End of - Headers==============================
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Has anyone used this buffer before? I plan on using this to make up a fixative to perfuse fix a rat. I will be looking at myelin of the sciatic nerve. Why does this buffer have such a high osmolarity? Any thoughts?
Pan American Advanced Studies Institute on Transmission Electron Microscopy in Materials Science
July 9 to July 22, 2006
Expenses paid Further information: http://www.pasi-tem2006.cl
At the University of Chile - in Santiago, Chile - a new field-emission TEM is being installed. This microscope will form the basis of a Pan American Advanced Studies Institute which will cover principles and applications of TEM to topics in materials and geological sciences. The Institute is funded by the National Science Foundation of the United States.
We invite applications from students and young researchers who wish to participate in this two-week intensive workshop. APPLICANTS WHO ARE ACCEPTED WILL HAVE THEIR EXPENSES PAID. The number of participants in the workshop will be limited to 48.
The lectures and laboratories (presented by a distinguished group of lecturers) will treat a wide range of topics: principles and applications of transmission electron microscopy; microanalysis; latest results and advances; and sample preparation.
The Institute is open to participants from any country in the Americas (including the U.S.A.). Participation by women and members of minority groups is greatly encouraged. Details of how to apply are given on the web site: http://www.pasi-tem2006.cl The closing date for applications is March 13, 2006
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 10, 22 -- From jae5-at-lehigh.edu Wed Feb 1 15:13:05 2006 10, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 10, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k11LD4QL023398 10, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 15:13:04 -0600 10, 22 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 10, 22 -- (authenticated bits=0) 10, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k11LD3Wn022378 10, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Feb 2006 16:13:03 -0500 10, 22 -- Message-ID: {43E1245F.1070609-at-lehigh.edu} 10, 22 -- Date: Wed, 01 Feb 2006 16:13:03 -0500 10, 22 -- From: Alwyn Eades {jae5-at-lehigh.edu} 10, 22 -- Organization: Lehigh University 10, 22 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 10, 22 -- X-Accept-Language: en-us, en 10, 22 -- MIME-Version: 1.0 10, 22 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 10, 22 -- Subject: Free TEM Workshop Materials and Geology 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit 10, 22 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 10, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
The Quanta 200 uses a W filament. Unless it is a SFEG. If there are no ion pumps, you should be able to use Nitrogen or He. If there is an ion pump, do not use He. It will kill the pump.
I found that for VP (20-120Pa) that N2 works fine and is better than air (less vacuum issues). So, for ESEM, I would think that N2 ought to be the gas of choice as well.
For VP mode, I do quant with EDAX Genesis using collected values at two pressure/vacuum values. This is their VIP option. I find good correlation between it and high vacuum mode. Which EDS are you using? Does it have an ESEM option mode?
gary g.
At 06:45 AM 2/1/2006, you wrote:
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it is a long time ago I have used a Tyrode-GA-mixture as a perfusion fixative for rat brain (selective hypothalamic target preparation containing ventricle III as well as magnocellular nuclear regions, providing "open" blood vessels/capillaries in that region; followed by a 3-dim reconstruction of those nuclear regions)....
Later on I had to use also such perfusion mixtures for retrograde perfusion of pig (renal) arteries...... I don't know or have at hand now the original receipt of } Tyrode {'s Mammalian Ringer solution, but have found in my methods/techniques collection } old { descriptions and protocols of the respective solutions and measurement data.
I have seen that your mixing formula varies only in the amount of Glucose......you indicate: 1 gm, in my descriptions I do have listed 10 grms.
On the other hand, when working with Tyrode's, initially I haven't mixed the buffer with sodium cacodylate, but instead with a very small amount of sodium-dihydrogenphosphate as follows: NaCl: 6.0 gm KCl: 0.2 gm CaCl2 (as CaCl2.2H2O) 0.2 (0.25g) gm MgCl2 (as MgCl2.6H2O) 0.1 (0.2. g) gm sodium-dihydrogenphosphate 0.05 gm Sodium-hydrogencarbonate(NaHCO3) 1.0 gm Glucose 10.0 gm ------------------------------------------------------------------------ ---- ad 1000 ml A.bidest (DD A.dest.) Phosphate(s) must be dissolved extra and should finally be added when all other } powder-substances { (! especially CaCl2 and MgCl2) are readily dissolved.
I have noted in the protocol: initial pH of the resulting solution: 8.4, approx. 275-277 mosmol (measuring also pH 8.14, mosmol: 310, 290, 285, 282, 295, 295).
pH-correction with approx. 2.95 ml 1 N HCl to pH. 7.2
The fixative for perfusion consisted of: 160 ml 25% glutaraldehyde (which means a final GA-concentration of 4% in the fixative) to be mixed with the above mentioned Tyrode stock solution to an endvolume of 1000 ml. If there formed a pale/ milky precipitate (which sometimes occured, perhaps due to a poor GA-quality with a high amount of aldehyde polymers - we had at that time in the late 1970ies - but most probably due to the Na2HPO4/NaH2PO4 !) the solution should be / has to be filtrated.
After doing so, I noted: pH ==} 8.4, 545-550 mosmol
Since the pH of that fix-mixture increased (????) to 8.4 with time again, it was necessary to adjust again with some ml of 1 N HCl to a pH of at least 7.7 (the solution now seemed to act quite well as a } buffer { system ! ) and finally, having adjusted the solution to pH 7.2 again, I then measured 1160 /1190 / 1200 mosmol.
As the following washing buffer I used at that time a Tyrode solution as given above, but added Glucose 25 g ad 1000 ml solution (which perhaps was wrong), and measured (after pH correction with approx. 1.3 ml of 1 N HCl to pH 7.2) 360 / 370 / 375 mosmol.
The 4% glutaraldehyde perhaps will contribute approx. 280 mosmol to the total osmolarity (if calculating from some own measurements and some tables found in the literaturefor 1, 1.5, 2.0 and 5 % GA-Solutions, respectively), the rest up to 1200 mosmol therefore must originate from the (more complete) dissociation of total ions dissolved in the tyrode's solution.
You state that the Tyrode solution you measured had 400/401 mosmol and is thought to be unusually high.
I assume that there is a contribution by the sodium-cacodylate you added (which is, if you use 10.7 g / 1000 ml, a final molarity of 0.05 mol) which could be in the range of 100 mosmol.
Consider also that due to more effective dissociation of ionic components in the solution the more diluted your fluid is, osmolarity will increase - not in a 1:1 mode (eg. Na-phosphate ions in Na2HPO4 and NaH2PO4 0,1 M will not have double the osmol amount of 0.05 M, which in fact will be higher due to more effective dissociation).
In my files I have other/additional data concerning the use of Tyrode's ringer solution, I you like, I could share those with you on request offline.
Best regards,
Wolfgang Muss OR Dr. Wolfgang Muss EM-Lab ====} with pride: 25 years in operation by 2nd of Feb. 2006 {==== Institute of Pathology, SALK (Salzburger Landeskliniken gemeinnuetzige GesmbH) Muellner Hauptstrasse 48 A-5020 SALZBURG AUSTRIA/EUROPE
and/or/alternatively
Paracelsus Medical Private University (PMU) Institute of Pathology Electron Microscopy Lab Muellner Hauptstrasse 48 A-5020 SALZBURG, Austria/Europe Phone work: +43+662+4482+4720 Mobile phone work:+43+662+4482-57704 Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o W.Muss") E-Mail work: W.Muss-at-SALK.at
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dyel-at-mail.nih.gov as well as the MIcroscopy Listserver --------------------------------------------------------------------------- Email: dyel-at-mail.nih.gov Name: Chip Dye Organization: NIH-NICHD Title-Subject: [Filtered] Tyrodes-Cacodylate Buffer
Question: Dear ListServer:
Has anyone used this buffer before? I plan on using this to make up a fixative to perfuse fix a rat. I will be looking at myelin of the sciatic nerve. Why does this buffer have such a high osmolarity? Any thoughts?
There are some things that are not clear about this problem. The question was written as if the use of water in an ESEM is a problem specifically for silicon drift detectors. Surely the problem is the same for all types of detector; the problem is not with the detector but with the window. In a system working correctly, the vacuum of the detector is quite separate from the vacuum of the chamber. So it does not matter what gas you use in the ESEM as long as the window is intact.
Does water in an ESEM cause the window of the detector to develop holes more quickly than, say, nitrogen in the ESEM? This could be the case, depending on the window design. We did have window failures in our ESEM, but we are told that this was a temporary problem and that the windows being sold now are just fine in an ESEM using water. Does anyone have specific knowledge on this?
Alwyn Eades
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
==============================Original Headers============================== 6, 25 -- From jae5-at-lehigh.edu Thu Feb 2 09:36:46 2006 6, 25 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12FakDr007269 6, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 09:36:46 -0600 6, 25 -- Received: from [128.180.55.141] (Dyn055141.mat.Lehigh.EDU [128.180.55.141]) 6, 25 -- (authenticated bits=0) 6, 25 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k12Fakid004491 6, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 6, 25 -- Thu, 2 Feb 2006 10:36:46 -0500 6, 25 -- Message-ID: {43E2270E.4050600-at-lehigh.edu} 6, 25 -- Date: Thu, 02 Feb 2006 10:36:46 -0500 6, 25 -- From: Alwyn Eades {jae5-at-lehigh.edu} 6, 25 -- Organization: Lehigh University 6, 25 -- User-Agent: Mozilla Thunderbird 1.0.2 (Windows/20050317) 6, 25 -- X-Accept-Language: en-us, en 6, 25 -- MIME-Version: 1.0 6, 25 -- To: hagglundk1-at-nku.edu, 6, 25 -- "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 6, 25 -- Subject: Re: [Microscopy] ESEM chamber gas choices 6, 25 -- References: {200602011533.k11FXYuO006193-at-ns.microscopy.com} 6, 25 -- In-Reply-To: {200602011533.k11FXYuO006193-at-ns.microscopy.com} 6, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 25 -- Content-Transfer-Encoding: 7bit 6, 25 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 6, 25 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Does anyone have experience with the Cytoviva imaging system. It looks to me (from their website at www.cytovita.com like it is a retrofit condenser. They claim a resolution better than 50 nm. In fact, their website says "The typical optical microscope provides a maximum theoretical resolution limit of 240nm. With resolution below 100nm and detection below 50nm, CytoViva allows you to see details never before possible with traditional microscopy. " Can someone explain to me how one can improve on the maximum theoretical resolution?
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
Dear Prof Phillips, perhaps the instrument of Cytoviva ( http://www.cytoviva.com ) is a further development of a technique I found in PNAS 2002 (if you want I can provide you with a pdf of this article). Unfortunately I have not followed that thread.....
FYI: Title of work:
Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast Alexander Egner, Stefan Jakobs, and Stefan W. Hell*
3370-3375 PNAS March 19, 2002 vol. 99 no. 6 By introducing beam-scanning multifocal multiphoton 4Pi-confocal microscopy, we have attained fast fluorescence imaging of live cells with axial super resolution. Rapid scanning of up to 64 pairs of interfering high-angle fields and subsequent confocal detection enabled us to perform three to five times finer optical sectioning than confocal microscopy. In conjunction with nonlinear image restoration, we demonstrate, to our knowledge for the first time, three-dimensional imaging of live eukaryotic cells at an equilateral resolution of ~ 100 nm. This imaging mode allowed us to reveal the morphology and size of the green fluorescent protein-labeled mitochondrial compartment of live Saccharomyces cerevisiae (bakers' yeast) growing on different carbon sources. Our studies show that mitochondria of cells grown on medium containing glycerol as the only carbon source, as opposed to glucose-grown cells, exhibit a strongly branched tubular reticulum. We determine the average tubular diameter and find that it increases from 339 +/- 5 nm to 360 +/- 4 nm when changing from glucose to glycerol, that is, from a fermentable to a nonfermentable carbon source. Moreover, this change is associated with a 2.8-fold increase of the surface of the reticulum, resulting in an average increase in volume of the mitochondrial compartment by a factor of 3.0 +/- 0.2. Best regards
Wolfgang Muss Salzburg
---------- Von: phillipst-at-missouri.edu[SMTP:phillipst-at-missouri.edu] Antwort an: phillipst-at-missouri.edu Gesendet: Donnerstag, 02. Februar 2006 18:44 An: W.Muss-at-salk.at Betreff: [Microscopy] Cytoviva system
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Does anyone have experience with the Cytoviva imaging system. It looks to me (from their website at www.cytovita.com like it is a retrofit condenser. They claim a resolution better than 50 nm. In fact, their website says "The typical optical microscope provides a maximum theoretical resolution limit of 240nm. With resolution below 100nm and detection below 50nm, CytoViva allows you to see details never before possible with traditional microscopy. " Can someone explain to me how one can improve on the maximum theoretical resolution?
Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
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Hi Alwyn,
My information on this is not definite but, my understanding is that different EDS manufacturers use different process for producing their detector windows. Consequently, there are different consequences between detectors depending on the gases used in the chamber. I can't see that there would be a difference between Si(Li) and SDD but I can understand a difference between manufacturers.
Best regards, -- Larry Stoter JEOL (UK) Ltd tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com
PLEASE NOTE 1. Any mail other than plain text will be automatically deleted. 2. Any mail, legitimate or not, apparently or actually from hotmail, netscape, yahoo or excite will automatically be deleted. 3. Mail with no subject or without a clear subject will be ignored :-)
==============================Original Headers============================== 5, 16 -- From larry-at-cymru.freewire.co.uk Thu Feb 2 14:51:22 2006 5, 16 -- Received: from get.freewire.net (get.freewire.net [195.184.229.250]) 5, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k12KpLst006559 5, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 2 Feb 2006 14:51:22 -0600 5, 16 -- Received: from [217.154.254.216] ([217.154.254.216]) 5, 16 -- by get.freewire.net (8.11.6/8.11.6) with ESMTP id k12KpH004925; 5, 16 -- Thu, 2 Feb 2006 20:51:17 GMT 5, 16 -- Mime-Version: 1.0 5, 16 -- Message-Id: {p06210200c0081f8a361d-at-[217.154.254.125]} 5, 16 -- In-Reply-To: {200602021537.k12FbpsN009683-at-ns.microscopy.com} 5, 16 -- References: {200602021537.k12FbpsN009683-at-ns.microscopy.com} 5, 16 -- Date: Thu, 2 Feb 2006 20:48:41 +0000 5, 16 -- To: jae5-at-lehigh.edu, Microscopy-at-MSA.Microscopy.Com 5, 16 -- From: Larry Stoter {larry-at-cymru.freewire.co.uk} 5, 16 -- Subject: [Microscopy] Re: ESEM chamber gas choices 5, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
} My information on this is not definite but, my understanding is that } different EDS manufacturers use different process for producing their } detector windows. Consequently, there are different consequences } between detectors depending on the gases used in the chamber. I can't } see that there would be a difference between Si(Li) and SDD but I can } understand a difference between manufacturers.
To bring this back to SDD, my understanding is that all SDD detector chips are manufactured by KETEK ... While individual SDD detector manufacturers will choose to employ different windows. I am still waiting to find out the source of information regarding a general query about SDDs' incompatibility with water vapor.
Genuinely, Michael Shaffer :o)
SEM/MLA Laboratory Coordinator http://www.mun.ca/creait/maf/ Inco Innovation Centre Memorial University St. John's, Newfoundland
==============================Original Headers============================== 7, 21 -- From michael-at-shaffer.net Thu Feb 2 16:31:55 2006 7, 21 -- Received: from ws6-5.us4.outblaze.com (ws6-5.us4.outblaze.com [205.158.62.152]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k12MVtbM017649 7, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2006 16:31:55 -0600 7, 21 -- Received: (qmail 9845 invoked from network); 2 Feb 2006 22:31:55 -0000 7, 21 -- Received: from unknown (HELO rarewolf1) (michael-at-shaffer.net-at-205.251.84.119) 7, 21 -- by ws6-5.us4.outblaze.com with SMTP; 2 Feb 2006 22:31:54 -0000 7, 21 -- From: "michael shaffer" {michael-at-shaffer.net} 7, 21 -- To: {larry-at-cymru.freewire.co.uk} , 7, 21 -- "MSA Microscopy list" {Microscopy-at-microscopy.com} 7, 21 -- Subject: RE: [Microscopy] Re: ESEM chamber gas choices 7, 21 -- Date: Thu, 2 Feb 2006 19:01:39 -0330 7, 21 -- Message-ID: {000201c62848$706e48f0$4f01a8c0-at-rarewolf1} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="us-ascii" 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Mailer: Microsoft Office Outlook 11 7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 7, 21 -- Thread-Index: AcYoOqzcXnO24mvMRVios5iM13rJLgADMGFA 7, 21 -- In-Reply-To: {200602022052.k12KqIrL007363-at-ns.microscopy.com} ==============================End of - Headers==============================
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Question: For those who do lab service for hire, how much is the going rate for SEM/EDX work? I only do work within our corporation but we're putting together a cost of what our work would cost if it was done outside.
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Email: mmstuason-at-yahoo.ca Name: Lizette Tuason
Title-Subject: [Filtered] Type of CO2 for CPD
Question: Hi everyone,
What type of CO2 do you use for CPD? The CO2 we currently have hooked up to our Denton DCP-1 is supercritical fluid extraction grade but it costs more than CAD$750. I looked up some online sites where people mentioned that they use just standard CO2 (in the US). Iķm not sure what standard CO2 is and what the equivalent would be here. Iķm buying from Praxair (Canada) and there are several categories that Iķm not sure which one I should be choosing.
Could anyone whoķs doing CPD tell me what CO2 you buy ń company and catalog number?
$200/hour....$150/hour.... $125/hour...$100/hour....pick a number.
This is a nice set of numbers but there is more to outsourcing than just the hourly rate...IMO. If a specimen is prepared in-house and then sent out-house, what is the out-house person to look for? Well, you will have to spend x hours writing a detailed procedural instruction for what you want to be analyzed. If the specimen deviates from this, then what? What is your writing time worth? Nothing?
The point is that there is great value in having the requester sitting by the SEM operator. Look here, look there, what is this, what is that? If you outsource, you will get exactly or less than what you ask for since the operator does not know what to do outside of your written request. If the job is so mundane (how many are?) then this is not a problem. The SEM is a very valuable tool for many areas of interest. But given a 12mm diameter specimen stub, which 2u are of the most interest? "I'll know it when I see it." Right. but they threw the specimen over the wall to the SEM folks. The results thrown back may not match up with what was needed. The variability of specimens means that there is no simple approach to evaluation. Plus, the requester is not at the SEM to know that they saw what they needed the first time.
gary g.
Disclaimer: I do not do this sort of ambiguous work. However, I do perform SEM analysis of unknown specimens and known specimens but I know what to look for.
At 05:44 PM 2/2/2006, you wrote:
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You are partially right about CytoViva: it is a retrofittable condenser fitted with special optics and an illuminator system.
Re: Resolution: I worked with Aetos at some length on the launch of CytoViva and have seen better than 90nm resolution (as measured with a Richardson test slide).
Re: how it works Dr. Vitaly Vodyanoy, the inventor currently has a paper in progress which will explain the physics. My personal observations lead me to believe that there is some sort of resonance effect rather than the traditional scattering or diffraction we normally use for imaging. The result looks a lot like fluorescence (bright object/dark background), without the need for staining. It also has an interesting ability to optically section (much like DIC or confocal). We were able to watch spirochetes actually invading cells as well as see more detail in bacteria and some special marine cells.
I encourage you to visit their website, if only to see some interesting images. Their technical applications specialist, Dr. Tom Hasling, is usually quite happy to run samples and Byron Cheatham, their sales manager, can probably arrange for a demo in your lab, if you are serious about potential purchase.
The system compares extremely well to other systems on the market in the $150K range, at about 10% the cost. ... and there's no cost for looking!
Hope this was helpful, Best regards, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 11:42 AM 2/2/2006, phillipst-at-missouri.edu wrote:
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==============================Original Headers============================== 22, 17 -- From bfoster-at-mme1.com Fri Feb 3 03:05:42 2006 22, 17 -- Received: from smtp109.sbc.mail.mud.yahoo.com (smtp109.sbc.mail.mud.yahoo.com [68.142.198.208]) 22, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1395gKj030224 22, 17 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 03:05:42 -0600 22, 17 -- Received: (qmail 98384 invoked from network); 3 Feb 2006 09:05:41 -0000 22, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.37.39 with login) 22, 17 -- by smtp109.sbc.mail.mud.yahoo.com with SMTP; 3 Feb 2006 09:05:41 -0000 22, 17 -- Message-Id: {7.0.1.0.0.20060203025348.0204e158-at-mme1.com} 22, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 22, 17 -- Date: Fri, 03 Feb 2006 03:05:43 -0600 22, 17 -- To: phillipst-at-missouri.edu, Microscopy Listserver {microscopy-at-microscopy.com} 22, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 22, 17 -- Subject: Re: [Microscopy] Cytoviva system 22, 17 -- In-Reply-To: {200602021742.k12HggDM022539-at-ns.microscopy.com} 22, 17 -- References: {200602021742.k12HggDM022539-at-ns.microscopy.com} 22, 17 -- Mime-Version: 1.0 22, 17 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
I'd recommend you also look into the Hi-Scope from Hirox-USA. They provide some really interesting imaging modes and have been well accepted by a number of major customers here in the US.
Caveat: MME has no financial interest in this product.
Hope this helpful,
Best regards, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 08:32 PM 1/30/2006, brad.huggins-at-bp.com wrote:
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==============================Original Headers============================== 15, 17 -- From bfoster-at-mme1.com Fri Feb 3 03:15:06 2006 15, 17 -- Received: from smtp107.sbc.mail.mud.yahoo.com (smtp107.sbc.mail.mud.yahoo.com [68.142.198.206]) 15, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k139F6LN007226 15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 03:15:06 -0600 15, 17 -- Received: (qmail 40367 invoked from network); 3 Feb 2006 09:15:06 -0000 15, 17 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.37.39 with login) 15, 17 -- by smtp107.sbc.mail.mud.yahoo.com with SMTP; 3 Feb 2006 09:15:06 -0000 15, 17 -- Message-Id: {7.0.1.0.0.20060201092440.01ff2348-at-mme1.com} 15, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 15, 17 -- Date: Fri, 03 Feb 2006 03:15:08 -0600 15, 17 -- To: brad.huggins-at-bp.com, Microscopy Listserver {microscopy-at-microscopy.com} 15, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 15, 17 -- Subject: Re: [Microscopy] viaWWW: LM, Anyone with "Digital Microscope" 15, 17 -- In-Reply-To: {200601310232.k0V2WUBj013521-at-ns.microscopy.com} 15, 17 -- References: {200601310232.k0V2WUBj013521-at-ns.microscopy.com} 15, 17 -- Mime-Version: 1.0 15, 17 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
You don't need the really pure stuff. We use food-grade, and in one trial found it cleaner (less water, oil, and particulates) than the expensive, "pure" siphon CO2. Just be sure to use a siphon-CO2 (comes in a cylinder with a siphon tube, so you get liquid, not gas, coming out), and spend the money for filters and dehydrating sieves.
Phil
We have the same Denton you do and use something what Airgas calls "bone dry", cat. No. CDBD200S with siphon tube. In addition, we use the Tousimis liquid CO2 filter (cat. No. 8784, I believe it helps). We pay about US$ 65 for a cylinder, if I remember well. This seems to be giving good results for CPD of biological material (mostly mouse embryos) for SEM.
Hope this helps,
Michal
mmstuason-at-yahoo.ca wrote:
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==============================Original Headers============================== 7, 21 -- From M_Jarnik-at-fccc.edu Fri Feb 3 08:51:46 2006 7, 21 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13EpjM8030588 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 08:51:46 -0600 7, 21 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 7, 21 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k13Epj1t020965 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 09:51:45 -0500 (EST) 7, 21 -- Message-ID: {43E36E02.2040108-at-fccc.edu} 7, 21 -- Date: Fri, 03 Feb 2006 09:51:46 -0500 7, 21 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 7, 21 -- Reply-To: M_Jarnik-at-fccc.edu 7, 21 -- Organization: Fox Chase Cancer Center 7, 21 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 7, 21 -- X-Accept-Language: en,cs 7, 21 -- MIME-Version: 1.0 7, 21 -- To: microscopy-at-microscopy.com 7, 21 -- Subject: Re: [Microscopy] viaWWW: type of CO2 do you use for CPD 7, 21 -- References: {200602030142.k131gpqb032061-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {200602030142.k131gpqb032061-at-ns.microscopy.com} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: Jones_e1269-at-yahoo.com Name: Jason Jones
Organization: The University of Texas Health Science Center at San Antonio
Title-Subject: [Filtered] Immuno EM
Question:
I am looking for a facility to do immuno-EM on the yeast Candida albicans for us. We're interested in intracellular localization of a soluble secretory protein, secreted aspartyl protease (Sap2p) in wild-type and a secretory mutant strain we have generated.
We have polyclonal antibodies to Sap2p, which have worked quite well in the published literature for immunoEM, and we have also 6X-His tagged this protein in our wild-type and mutant strains.
IWe do not have immunoEM available as a core facility here.
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Email: df-at-donnforbes.com Name: Donn Forbes
Organization: Arasil, Inc.
Title-Subject: [Filtered] SEM for BWA Detection
Question: Why isn't SEM a standard technology for detecting and identifying viruses and bacteria used as biological warfare agents?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bcarrington-at-slfc.org) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, February 3, 2006 at 09:15:17 ---------------------------------------------------------------------------
Email: bcarrington-at-slfc.org Name: Brenda Carrington
Organization: The Learning Center
Education: K-8 Grade Grammar School
Location: Chesterfeild MO (St. Louis area)
Question: We are haing a microscope day on Feb. 27 from 10:00-3:00. I don't know how to contact a local person to assist us. We have over 100 kids grades 2-9. We will be studying microbes and microscopes and using the GEMS materials.
We have inherited lots of copper TEM grids that are decades old. Many of them have a dark patina on them, and, on those grids, we have been losing our sections during alcohol-based uranyl acetate staining. The sections remain when we use water-based uranyl acetate, however. We have tried sonicating the grids in ethanol, acetone, or chloroform, none of which has solved our problem.
Does anyone have any suggestions for cleaning them so that we will not lose our sections during staining, or should we pitch them? We really do prefer to stain with alcohol- based uranyl acetate, since it provides a more intense staining.
As always, thanks for any suggestions you might have.
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School 4643 Medical Science Building II 1301 Catherine Ann Arbor, MI 48109-0616 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006 7, 17 -- Received: from pushingtin.mr.itd.umich.edu (pushingtin.mr.itd.umich.edu [141.211.14.78]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LmwV1020156 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 15:48:58 -0600 7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu [141.214.170.47]) 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id k13LmvJZ004383; 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2) 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu} 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu} 7, 17 -- Content-Transfer-Encoding: 7bit 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 7, 17 -- Subject: Cleaning TEM grids 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500 7, 17 -- To: microscopy-at-msa.microscopy.com 7, 17 -- X-Mailer: Apple Mail (2.746.2) ==============================End of - Headers==============================
Hi Dotty, I would try putting your grids into a drying oven for at least 5 minutes after you cut your ultrathin sections. After they are totally dried down, you can take them out. If you forget and leave your grids in the oven overnight, it doesn't seem to hurt anything at all. The heat seems to bond the sections to the grids very well, such that I've never had an experience of sections coming off the grids, regardless of the state of the grids.
To clean my grids, I just dip them into concentrated sodium hydroxide for a few seconds, and then rinse them by dipping them a few times in distilled water just before I pick up the sections. It sort of etches the grids.
Garry Burgess Charge Technologist - Electron Microscopy Health Science Centre Winnipeg, Canada
Dear listers,
We have inherited lots of copper TEM grids that are decades old. Many of them have a dark patina on them, and, on those grids, we have been losing our sections during alcohol-based uranyl acetate staining. The sections remain when we use water-based uranyl acetate, however. We have tried sonicating the grids in ethanol, acetone, or chloroform, none of which has solved our problem.
Does anyone have any suggestions for cleaning them so that we will not lose our sections during staining, or should we pitch them? We really do prefer to stain with alcohol- based uranyl acetate, since it provides a more intense staining.
As always, thanks for any suggestions you might have.
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School 4643 Medical Science Building II 1301 Catherine Ann Arbor, MI 48109-0616 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006 7, 17 -- Received: from pushingtin.mr.itd.umich.edu (pushingtin.mr.itd.umich.edu [141.211.14.78]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LmwV1020156 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 15:48:58 -0600 7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu [141.214.170.47]) 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id k13LmvJZ004383; 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2) 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu} 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu} 7, 17 -- Content-Transfer-Encoding: 7bit 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 7, 17 -- Subject: Cleaning TEM grids 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500 7, 17 -- To: microscopy-at-msa.microscopy.com 7, 17 -- X-Mailer: Apple Mail (2.746.2) ==============================End of - Headers==============================
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==============================Original Headers============================== 14, 20 -- From GBurgess-at-exchange.hsc.mb.ca Fri Feb 3 16:08:11 2006 14, 20 -- Received: from hscxntmx0003.hsc.mb.ca (hscxntmx0003.hsc.mb.ca [142.233.100.122]) 14, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13M8A8U017896 14, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 16:08:11 -0600 14, 20 -- Received: from hscxntmx0007.hsc.mb.ca (unverified [172.16.6.179]) by 14, 20 -- hscxntmx0003.hsc.mb.ca(Vircom SMTPRS 4.0.346.0) with ESMTP id 14, 20 -- {B0017955251-at-hscxntmx0003.hsc.mb.ca} for {Microscopy-at-microscopy.com} ;Fri, 14, 20 -- 3 Feb 2006 16:08:04 -0600 14, 20 -- Received: by hscxntmx0007.hsc.mb.ca with Internet Mail Service 14, 20 -- (5.5.2653.19)id {DXXMZXB1} ; Fri, 3 Feb 2006 16:08:43 -0600 14, 20 -- Message-ID: 14, 20 -- {00A937989100304A83A058F6C45873FF32A393-at-hscxntmx0005.hsc.mb.ca} 14, 20 -- Date: Fri, 3 Feb 2006 16:04:40 -0600 14, 20 -- From: Garry Burgess {GBurgess-at-exchange.hsc.mb.ca} 14, 20 -- Subject: RE: [Microscopy] Cleaning TEM grids 14, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 14, 20 -- X-Mailer: Internet Mail Service (5.5.2653.19) 14, 20 -- MIME-Version: 1.0 14, 20 -- Content-Type: text/plain; 14, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
We clean our grids in acid alcohol, a mixture of 3% HCL in 95% ethanol. Cover the grids in a small shell vial with the acid alcohol, swirl intermittantly for 30 to 40 seconds or so and rinse well with 95% ethanol. If the grids have a very dark patina then clean for a longer time. You'll know when they are done because they'll have a shiny, bright copper finish. We usually clean many grids at a time, but you could clean them one by one by dipping the grid in the acid alcohol and then rinsing in a stream of 95% ETOH just before you use them. This works better on grids that have been cleaned, but have set around for a while, long enough to have developed a small oxidized layer. Dark patinas like the grids you have will probably need to be put into a vial and cleaned as described above.
dsoren-at-umich.edu wrote:
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-- -- ================================================================== Greg Strout Electron Microscopist, University of Oklahoma WWW Virtual Library for Microscopy: http://www.ou.edu/research/electron/www-vl/ e-mail: gstrout-at-ou.edu Opinions expressed herein are mine and not necessarily those of the University of Oklahoma ==================================================================
==============================Original Headers============================== 6, 21 -- From gstrout-at-ou.edu Fri Feb 3 17:33:43 2006 6, 21 -- Received: from c3p0.ou.edu (mail.zero.ou.edu [129.15.0.75]) 6, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13NXhBV028012 6, 21 -- for {Microscopy-at-Sparc5.Microscopy.Com} ; Fri, 3 Feb 2006 17:33:43 -0600 6, 21 -- Received: from [127.0.0.1] ([129.15.38.202]) 6, 21 -- by c3p0.ou.edu (Sun Java System Messaging Server 6.1 HotFix 0.11 (built Jan 28 6, 21 -- 2005)) with ESMTP id {0IU400EHDXG5Q5A0-at-c3p0.ou.edu} for 6, 21 -- Microscopy-at-Sparc5.Microscopy.Com; Fri, 03 Feb 2006 17:33:41 -0600 (CST) 6, 21 -- Date: Fri, 03 Feb 2006 17:33:40 -0600 6, 21 -- From: Greg Strout {gstrout-at-ou.edu} 6, 21 -- Subject: Re: [Microscopy] Cleaning TEM grids 6, 21 -- In-reply-to: {200602032150.k13LoAHd025474-at-ns.microscopy.com} 6, 21 -- To: Microscopy List Server {Microscopy-at-ns.Microscopy.Com} , dsoren-at-umich.edu 6, 21 -- Message-id: {43E3E854.7040107-at-ou.edu} 6, 21 -- MIME-version: 1.0 6, 21 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-transfer-encoding: 7BIT 6, 21 -- X-Accept-Language: en-us, en 6, 21 -- References: {200602032150.k13LoAHd025474-at-ns.microscopy.com} 6, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 21 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
Try soaking a few grids in store grade ammonia for about 1/2 hr to 1 hr to see if the patina is removed at all. Rinse well in distilled water and see what happens.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: dsoren-at-umich.edu [mailto:dsoren-at-umich.edu] Sent: Friday, February 03, 2006 1:53 PM To: Walck-at-SouthBayTech.com
Dear listers,
We have inherited lots of copper TEM grids that are decades old. Many of them have a dark patina on them, and, on those grids, we have been losing our sections during alcohol-based uranyl acetate staining. The sections remain when we use water-based uranyl acetate, however. We have tried sonicating the grids in ethanol, acetone, or chloroform, none of which has solved our problem.
Does anyone have any suggestions for cleaning them so that we will not lose our sections during staining, or should we pitch them? We really do prefer to stain with alcohol- based uranyl acetate, since it provides a more intense staining.
As always, thanks for any suggestions you might have.
Dotty Sorenson
Dorothy Sorenson Microscopy and Image-analysis Laboratory Department of Cell and Developmental Biology University Of Michigan Medical School 4643 Medical Science Building II 1301 Catherine Ann Arbor, MI 48109-0616 (734)763-1170 FAX (734)763-1166
==============================Original Headers============================== 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006 7, 17 -- Received: from pushingtin.mr.itd.umich.edu (pushingtin.mr.itd.umich.edu [141.211.14.78]) 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k13LmwV1020156 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 15:48:58 -0600 7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu [141.214.170.47]) 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id k13LmvJZ004383; 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2) 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu} 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu} 7, 17 -- Content-Transfer-Encoding: 7bit 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu} 7, 17 -- Subject: Cleaning TEM grids 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500 7, 17 -- To: microscopy-at-msa.microscopy.com 7, 17 -- X-Mailer: Apple Mail (2.746.2) ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From walck-at-southbaytech.com Fri Feb 3 20:16:08 2006 17, 27 -- Received: from ylpvm15.prodigy.net (ylpvm15-ext.prodigy.net [207.115.57.46]) 17, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k142G7Bg007081 17, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 20:16:08 -0600 17, 27 -- Received: from pimout7-ext.prodigy.net (pimout7-int.prodigy.net [207.115.4.147]) 17, 27 -- by ylpvm15.prodigy.net (8.12.10 outbound/8.12.10) with ESMTP id k142GKEV029956 17, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Feb 2006 21:16:20 -0500 17, 27 -- X-ORBL: [64.169.193.90] 17, 27 -- Received: from dynamicbl8uno3 (adsl-64-169-193-90.dsl.lsan03.pacbell.net [64.169.193.90]) 17, 27 -- by pimout7-ext.prodigy.net (8.13.4 outbound domainkey aix/8.13.4) with ESMTP id k142Fvmf095186; 17, 27 -- Fri, 3 Feb 2006 21:16:02 -0500 17, 27 -- From: "Scott Walck" {walck-at-southbaytech.com} 17, 27 -- To: {dsoren-at-umich.edu} 17, 27 -- Cc: {Microscopy-at-microscopy.com} 17, 27 -- Subject: RE: [Microscopy] Cleaning TEM grids 17, 27 -- Date: Fri, 3 Feb 2006 18:16:01 -0800 17, 27 -- Message-ID: {007801c62930$f81347e0$7801a8c0-at-dynamicbl8uno3} 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="us-ascii" 17, 27 -- Content-Transfer-Encoding: 7bit 17, 27 -- X-Priority: 3 (Normal) 17, 27 -- X-MSMail-Priority: Normal 17, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 17, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 17, 27 -- In-Reply-To: {200602032153.k13LrLc5007111-at-ns.microscopy.com} 17, 27 -- Importance: Normal ==============================End of - Headers==============================
I've always cleaned grids by quickly passing through the flame of an alcohol burner. Quick and easy. Works on my old grids, though they aren't decades old!
Diana van Driel Dept Ophthalmology Sydney University GPO Box 4337 Sydney, NSW AUSTRALIA 2001
Phone 61 2 93827278 Mobile 0423 151614 FAX 61 2 93827318 On 4 Feb 2006, at 8:50 AM, dsoren-at-umich.edu wrote:
} } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear listers, } } We have inherited lots of copper TEM grids that are decades old. } Many of them have a dark patina on them, and, on those grids, we have } been losing our sections during alcohol-based uranyl acetate } staining. The sections remain when we use water-based uranyl } acetate, however. We have tried sonicating the grids in ethanol, } acetone, or chloroform, none of which has solved our problem. } } Does anyone have any suggestions for cleaning them so that we will } not lose our sections during staining, or should we pitch them? We } really do prefer to stain with alcohol- based uranyl acetate, since } it provides a more intense staining. } } As always, thanks for any suggestions you might have. } } Dotty Sorenson } } Dorothy Sorenson } Microscopy and Image-analysis Laboratory } Department of Cell and Developmental Biology } University Of Michigan Medical School } 4643 Medical Science Building II } 1301 Catherine } Ann Arbor, MI 48109-0616 } (734)763-1170 } FAX (734)763-1166 } } } ==============================Original } Headers============================== } 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006 } 7, 17 -- Received: from pushingtin.mr.itd.umich.edu } (pushingtin.mr.itd.umich.edu [141.211.14.78]) } 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k13LmwV1020156 } 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 } 15:48:58 -0600 } 7, 17 -- Received: from [141.214.170.47] } (host-47.subnet-170.med.umich.edu [141.214.170.47]) } 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id } k13LmvJZ004383; } 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500 } 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2) } 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu} } 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu} } 7, 17 -- Content-Transfer-Encoding: 7bit } 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu} } 7, 17 -- Subject: Cleaning TEM grids } 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500 } 7, 17 -- To: microscopy-at-msa.microscopy.com } 7, 17 -- X-Mailer: Apple Mail (2.746.2) } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 20 -- From dianavd-at-eye.usyd.edu.au Sun Feb 5 22:06:54 2006 6, 20 -- Received: from galen.med.usyd.edu.au (machaon.med.usyd.edu.au [129.78.36.30]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1646r3u022150 6, 20 -- for {Microscopy-at-microscopy.com} ; Sun, 5 Feb 2006 22:06:53 -0600 6, 20 -- Received: from [129.78.203.144] (helo=[129.78.203.144]) 6, 20 -- by galen.med.usyd.edu.au with esmtp (Exim 4.50) 6, 20 -- id 1F5xYY-0004NY-9a 6, 20 -- for Microscopy-at-microscopy.com; Mon, 06 Feb 2006 15:00:52 +1100 6, 20 -- Mime-Version: 1.0 (Apple Message framework v623) 6, 20 -- In-Reply-To: {200602032150.k13Loevb027732-at-ns.microscopy.com} 6, 20 -- References: {200602032150.k13Loevb027732-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 20 -- Message-Id: {25a1f04e5a26d06c447521476709da97-at-eye.usyd.edu.au} 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- From: Diana van Driel {dianavd-at-eye.usyd.edu.au} 6, 20 -- Subject: Re: [Microscopy] Cleaning TEM grids 6, 20 -- Date: Mon, 6 Feb 2006 15:06:32 +1100 6, 20 -- To: Microscopy {Microscopy-at-microscopy.com} 6, 20 -- X-Mailer: Apple Mail (2.623) 6, 20 -- X-Spam-Score: -5.9 (-----) ==============================End of - Headers==============================
Dear Dotty, We were in the same situation some years ago. We cleaned the grids in the following way: - put the grids into small beaker (25ml) filled with 5% - 10% hydrochloric acid - let the grids to clean for some minutes, gently shake several times (at the end of cleaning the grids should be shiny gold) - remove the cleaning hydrochloric acid solution and wash the grids several times with distilled water - remove the distilled water as much as possible from the beaker and replace it with acetone and let stand for some time - pour the acetone with the grids into clean glass Petri dish filled with filter paper - remove the filter paper with the grids and put it into another glass Petri dish and let dry out the rest of acetone - that's all
The troubles with losing the sections during the staining could be overcome by making the grids sticky: - put 10 ml of chloroform into 25 ml glass beaker - cut about 5 cm of 3M Magic Scotch tape and wash it in the chloroform - remove the rest of the tape from the beaker - put the grids onto filter paper and drop the "sticky solution" on them using Pasteur pipette - let the grids dry and use them for collecting the sections
Best regards from Prague Oldrich ---------------------------------------- Oldrich Benada Institute of Microbiology Acad. Sci. CR Videnska 1083 CZ - 142 20 Prague 4 Czech Republic ---------------------------------------
} } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } } We have inherited lots of copper TEM grids that are decades old. } Many of them have a dark patina on them, and, on those grids, we have } been losing our sections during alcohol-based uranyl acetate } staining. The sections remain when we use water-based uranyl } acetate, however. We have tried sonicating the grids in ethanol, } acetone, or chloroform, none of which has solved our problem. } } Does anyone have any suggestions for cleaning them so that we will } not lose our sections during staining, or should we pitch them? We } really do prefer to stain with alcohol- based uranyl acetate, since } it provides a more intense staining. } } As always, thanks for any suggestions you might have. } } Dotty Sorenson } } Dorothy Sorenson } Microscopy and Image-analysis Laboratory } Department of Cell and Developmental Biology } University Of Michigan Medical School } 4643 Medical Science Building II } 1301 Catherine } Ann Arbor, MI 48109-0616 } (734)763-1170 } FAX (734)763-1166 } } } ==============================Original } Headers============================== } 7, 17 -- From dsoren-at-umich.edu Fri Feb 3 15:48:58 2006 } 7, 17 -- Received: from pushingtin.mr.itd.umich.edu } (pushingtin.mr.itd.umich.edu [141.211.14.78]) } 7, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k13LmwV1020156 } 7, 17 -- for {microscopy-at-msa.microscopy.com} ; Fri, 3 Feb 2006 15:48:58 } -0600 } 7, 17 -- Received: from [141.214.170.47] (host-47.subnet-170.med.umich.edu } [141.214.170.47]) } 7, 17 -- by pushingtin.mr.itd.umich.edu (smtp) with ESMTP id } k13LmvJZ004383; } 7, 17 -- Fri, 3 Feb 2006 16:48:57 -0500 } 7, 17 -- Mime-Version: 1.0 (Apple Message framework v746.2) } 7, 17 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; } format=flowed } 7, 17 -- Message-Id: {F847072A-3C48-4995-B594-5900360673CB-at-umich.edu} } 7, 17 -- Cc: Sasha Meshinchi {meshin-at-umich.edu} } 7, 17 -- Content-Transfer-Encoding: 7bit } 7, 17 -- From: Dorothy Sorenson {dsoren-at-umich.edu} } 7, 17 -- Subject: Cleaning TEM grids } 7, 17 -- Date: Fri, 3 Feb 2006 16:47:15 -0500 } 7, 17 -- To: microscopy-at-msa.microscopy.com } 7, 17 -- X-Mailer: Apple Mail (2.746.2) } ==============================End of - } Headers============================== }
==============================Original Headers============================== 9, 25 -- From benada-at-biomed.cas.cz Mon Feb 6 03:33:26 2006 9, 25 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 9, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k169XPV1003261 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 03:33:26 -0600 9, 25 -- Received: from mail2.biomed.cas.cz (localhost [127.0.0.1]) 9, 25 -- by biomed.cas.cz (8.13.3+Sun/8.13.3) with ESMTP id k169V0cA006059; 9, 25 -- Mon, 6 Feb 2006 10:31:00 +0100 (CET) 9, 25 -- Received: from 147.231.44.104 9, 25 -- (SquirrelMail authenticated user benada) 9, 25 -- by mail2.biomed.cas.cz with HTTP; 9, 25 -- Mon, 6 Feb 2006 10:31:01 +0100 (CET) 9, 25 -- Message-ID: {1280.147.231.44.104.1139218261.squirrel-at-mail2.biomed.cas.cz} 9, 25 -- In-Reply-To: {200602032152.k13LqKBS002784-at-ns.microscopy.com} 9, 25 -- References: {200602032152.k13LqKBS002784-at-ns.microscopy.com} 9, 25 -- Date: Mon, 6 Feb 2006 10:31:01 +0100 (CET) 9, 25 -- Subject: Re: [Microscopy] Cleaning TEM grids 9, 25 -- From: =?iso-8859-2?Q?Old=F8ich_Benada?= {benada-at-biomed.cas.cz} 9, 25 -- To: dsoren-at-umich.edu 9, 25 -- Cc: microscopy-at-microscopy.com 9, 25 -- User-Agent: SquirrelMail/1.4.4 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Type: text/plain;charset=iso-8859-2 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-Priority: 3 (Normal) 9, 25 -- Importance: Normal ==============================End of - Headers==============================
Overview: Schafer Corporation, Sunol, California, is seeking a skilled and innovative individual to add to our mass spectrometry group. SVL performs materials characterization and related analytical services on commercial and government contracts. The activities of the group include chemical and elemental analysis of materials on a production basis, maintenance of several mass spectrometers and ancillary equipment, development of new or improved MS analysis techniques, and quality assurance of analytical data.
Schafer is a technically strong firm with a reputation for quality and integrity. Schafer's reputation is a direct result of our dedicated, motivated, talented, and creative staff that is responsible for developing our outstanding business relationships with our customers. Our technical capabilities are vast and growing to provide innovations for the future.
The successful candidate will work as part of a team responsible for processing and analyzing small samples using laboratory instrumentation, primarily Thermal Ionization and/or Secondary Ion Mass Spectrometers.
Responsibilities: Duties include instrument operation and data interpretation; filament construction and preparation; standards loading; as well as high vacuum, high voltage, and cryogenic system maintenance. The successful candidate should have or be able to develop skills to process samples, maintain equipment, and assist in developing and optimizing analytical techniques.
Qualifications: The ideal candidate will have technical training in an appropriate physical sciences field and related experience in a scientific laboratory, preferably operating mass spectrometers or other analytical equipment. Ability to work independently as well as part of a team is required. Good customer service focus and commitment to quality are required. Experience in the use of a light microscope, and sufficient physical dexterity to perform micromanipulation tasks is highly desired, as is knowledge of high vacuum and cryogenic principles. Schafer is looking for people that have established safe laboratory skills and exceptional aptitude for details including accurate record keeping. Experience in laboratory data management (word processing, spread sheets and databases) is also desired. Experience with mechanical systems or machining skills would be helpful. Other qualifications include: . AA degree plus 10 years experience or Bachelor's degree in physical science or engineering plus two years of technical experience. . Demonstrated ability to solve technical problems. . Experience in data evaluation and quality control. . Must be a US citizen with the ability to obtain government security clearance. Apply at: http://www.schafercorp.com/Careers/open.htm http://jobs-schafer.icims.com/schafer_jobs/jobs/candidate/job.jsp?jobid=1106 &mode=view
==============================Original Headers============================== 6, 20 -- From dsams-at-schaferlabs.com Mon Feb 6 19:16:53 2006 6, 20 -- Received: from mail.schaferlabs.com (mail.schaferlabs.com [64.168.91.154]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k171GkxV006745 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 19:16:52 -0600 6, 20 -- Received: from dsamsws ([10.10.10.1]) 6, 20 -- by mail.schaferlabs.com (8.12.11/8.12.11) with ESMTP id k171ANrk020002 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 17:10:34 -0800 6, 20 -- Message-Id: {200602070110.k171ANrk020002-at-mail.schaferlabs.com} 6, 20 -- From: "David Sams" {dsams-at-schaferlabs.com} 6, 20 -- To: {Microscopy-at-microscopy.com} 6, 20 -- Subject: Mass Spectrometry Scientist / Senior Engineer at Schafer Corporation 6, 20 -- Date: Mon, 6 Feb 2006 17:16:18 -0800 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="US-ASCII" 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 6, 20 -- In-reply-to: 6, 20 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.2670 6, 20 -- Thread-Index: AcVrdaWs9qR/q27tSgm/q8Axr3R/miwGADdQAAH4L3AAM6jdwAPH8fGQ ==============================End of - Headers==============================
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Email: RANGETS-at-AOL.COM Name: BEN GHAFFAARI
Organization: RANGE TECHNICAL
Title-Subject: [Filtered] NEED SCHEMATICS
Question: DOES ANYONE HAVE AN EXTRA SET OF "LEO" 440 SCHEMATICS, WE CAN BUY OR PURCHASE, WHEN WE TRY TO CONTACT THE MFR, WE DO NOT GET A RESPONSE, SO MAYBE WE ARE USING AN INCORRECT CONTACT. IF SOMEONE KNOWS WHO WE CONTACT FOR "LEO" PARTS ANY SUGGESTIONS WILL BE APPRECIATED
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mcmahojt-at-ccf.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mcmahojt-at-ccf.org Name: Jim McMahon
Organization: Cleveland Clinic Foundation
Title-Subject: [Filtered] Spurr Resin
Question: We have been having great difficulty with the newly formulated Spurr Resin and in particular the ERL component. Not only is Spurr no longer low viscosity but we find that its penetration and polymerization to be far inferior to the original. We are prepared to abandon the resin in favor of another such as PolyBed or Araldite. But first I would like to know if anyone else has had similar problems and was able to solve them.
for what it is worth, i've recently re-tried a commercial version of Epon 812 on a cell suspension. i found the cells would not settle through the resin mix, and could not be pelleted. a parallel embedment in the new Spurr with ERL behaved very well. in short, whatever is said about low viscosity editions of Epon, neither they, nor the original were ever thin enough to allow embedments of free cells and i personally found them more than a little difficult to cut. but then i've always found Epon, in whatever formulation, prone to static and difficult to section.
on the other hand, the problem may really be an issue of density of the medium, causing the the cells to be bouyant in the medium. does anyone want to contribute knowledge on that?
having said that, i do find the new ERL component to be a little brittle, but very sectionable.
paul
==============================Original Headers============================== 7, 21 -- From paul_hazelton-at-umanitoba.ca Mon Feb 6 23:49:49 2006 7, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k175niDC005523 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2006 23:49:48 -0600 7, 21 -- Received: from [130.179.152.145] (cvx-082.cc.umanitoba.ca [130.179.152.145]) 7, 21 -- (authenticated bits=0) 7, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k175ndFk026699; 7, 21 -- Mon, 6 Feb 2006 23:49:40 -0600 (CST) 7, 21 -- Message-ID: {43E9865F.3010305-at-umanitoba.ca} 7, 21 -- Date: Tue, 07 Feb 2006 23:49:19 -0600 7, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 7, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- To: mcmahojt-at-ccf.org 7, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] viaWWW: Spurr Resin 7, 21 -- References: {200602070432.k174Wx2a027920-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {200602070432.k174Wx2a027920-at-ns.microscopy.com} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We would need some to improve cell attachment for a time course experiment. Surprisingly, we were not able to locate any coverslips (unlike slides). Does anybody have recommendations? (Of course, we can always make our own.)
Thanks,
Michael
==============================Original Headers============================== 6, 19 -- From M_Jarnik-at-fccc.edu Tue Feb 7 08:35:31 2006 6, 19 -- Received: from azah.fccc.edu (azah.fccc.edu [131.249.4.237]) 6, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17EZVJJ022275 6, 19 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:35:31 -0600 6, 19 -- Received: from fccc.edu (emf4.fccc.edu [131.249.9.170]) 6, 19 -- by azah.fccc.edu (8.12.11/8.12.11) with ESMTP id k17EZU5d016976 6, 19 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 09:35:31 -0500 (EST) 6, 19 -- Message-ID: {43E8B032.7060002-at-fccc.edu} 6, 19 -- Date: Tue, 07 Feb 2006 09:35:30 -0500 6, 19 -- From: Michal Jarnik {M_Jarnik-at-fccc.edu} 6, 19 -- Reply-To: M_Jarnik-at-fccc.edu 6, 19 -- Organization: Fox Chase Cancer Center 6, 19 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 6, 19 -- X-Accept-Language: en,cs 6, 19 -- MIME-Version: 1.0 6, 19 -- To: microscopy-at-msa.microscopy.com 6, 19 -- Subject: Poly-L-lysine coated coverslips 6, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have recently encountered problems with Spurr as well. I embed 100 micron thick Vibratome sections of brain so penetration should not be a problem. I am using the same processing proceedure I have used for many, many years on much larger tissue blocks. The center of the sections seems to be poorly infiltrated and the block is very brittle. Cutting intact 1 micron sections is nearly impossible. The viscosity seems 'normal', in other words, like I am used to with Spurr and I am not familiar with any 'new' or 'improved' version of the mix or its components. I will cut some more blocks and talk to my supplier (a very reputable EM supplier I have used for 30 years) to see if I can shed some light on this problem.
Geoff
mcmahojt-at-ccf.org wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 8, 32 -- From mcauliff-at-umdnj.edu Tue Feb 7 08:43:50 2006 8, 32 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 8, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17Ehnhp031730 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:43:49 -0600 8, 32 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 8, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 9D1FD4BE3B 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:43:49 -0600 (CST) 8, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 8, 32 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 59FA44BE34 8, 32 -- for {microscopy-at-msa.microscopy.com} ; Tue, 7 Feb 2006 08:43:48 -0600 (CST) 8, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 8, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 8, 32 -- id {0IUB00D01MP310-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 8, 32 -- for microscopy-at-msa.microscopy.com; Tue, 07 Feb 2006 09:43:44 -0500 (EST) 8, 32 -- Received: from [127.0.0.1] ([10.138.2.240]) 8, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 8, 32 -- 2004)) with ESMTP id {0IUB00BK4NAVAK-at-Polaris.umdnj.edu} ; Tue, 8, 32 -- 07 Feb 2006 09:37:43 -0500 (EST) 8, 32 -- Date: Tue, 07 Feb 2006 09:37:06 -0500 8, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 8, 32 -- Subject: Re: [Microscopy] Spurr Resin 8, 32 -- In-reply-to: {200602070432.k174W3M3025365-at-ns.microscopy.com} 8, 32 -- To: mcmahojt-at-ccf.org, MicroscopyListserver {microscopy-at-msa.microscopy.com} , 8, 32 -- paul_hazelton-at-umanitoba.ca 8, 32 -- Message-id: {43E8B092.8030903-at-umdnj.edu} 8, 32 -- MIME-version: 1.0 8, 32 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 8, 32 -- Content-transfer-encoding: 7BIT 8, 32 -- X-Accept-Language: en-us, en 8, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 8, 32 -- Gecko/20040804 Netscape/7.2 (ax) 8, 32 -- References: {200602070432.k174W3M3025365-at-ns.microscopy.com} ==============================End of - Headers==============================
Depends on what you want to do. If it is for EM you can use the Thermonox coverslips. I think all EM suppliers sell them, along with the general suppliers.
However, if you want to do IF or confocal work the thermonox will quench the reactions somewhat so you will have to do glass. I've used straight glass, but many cell lines will not stick well to glass. Undoubtably someone will provide the name of a supplier of pre-treated slips if they are out there somewhere.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 9, 21 -- From paul_hazelton-at-umanitoba.ca Tue Feb 7 08:53:50 2006 9, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17ErnB5008760 9, 21 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 08:53:49 -0600 9, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 9, 21 -- (authenticated bits=0) 9, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k17ErhaH011897; 9, 21 -- Tue, 7 Feb 2006 08:53:44 -0600 (CST) 9, 21 -- Message-ID: {43E8B474.8040005-at-umanitoba.ca} 9, 21 -- Date: Tue, 07 Feb 2006 08:53:40 -0600 9, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 9, 21 -- X-Accept-Language: en-us, en 9, 21 -- MIME-Version: 1.0 9, 21 -- To: M_Jarnik-at-fccc.edu 9, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 9, 21 -- Subject: Re: [Microscopy] Poly-L-lysine coated coverslips 9, 21 -- References: {200602071438.k17EcWLj026119-at-ns.microscopy.com} 9, 21 -- In-Reply-To: {200602071438.k17EcWLj026119-at-ns.microscopy.com} 9, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Regarding the brittle nature of Spurrs resin due to the new ERL component, we have solved our problems by combining the components according to the "soft" recipe:
ERL 10g DER 7g NSA 26g DMAE 0.4ml
This mixture results in blocks about as hard as the old "hard" formulation using VCD. We have not noticed a lack of tissue penetration during infiltration, but we may be less sensitive to this issue. Polymerization is still at 70 deg C for 17 hours.
Best wishes,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3102 S.W. Sam Jackson Park Road Portland, Oregon 97239 503-221-3434 drk-at-shcc.org
-----Original Message----- X-from: mcmahojt-at-ccf.org [mailto:mcmahojt-at-ccf.org] Sent: Monday, February 06, 2006 8:38 PM To: drk-at-SHCC.org
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Email: mcmahojt-at-ccf.org Name: Jim McMahon
Organization: Cleveland Clinic Foundation
Title-Subject: [Filtered] Spurr Resin
Question: We have been having great difficulty with the newly formulated Spurr Resin and in particular the ERL component. Not only is Spurr no longer low viscosity but we find that its penetration and polymerization to be far inferior to the original. We are prepared to abandon the resin in favor of another such as PolyBed or Araldite. But first I would like to know if anyone else has had similar problems and was able to solve them.
I think that they are responding in their own way. Their answer is "No."
gary g.
At 08:30 PM 2/6/2006, you wrote:
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==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Tue Feb 7 10:06:45 2006 9, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k17G6jtp028532 9, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 10:06:45 -0600 9, 20 -- Received: (qmail 12166 invoked from network); 7 Feb 2006 08:06:43 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 12163, pid: 12164, t: 0.1441s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 20 -- by qsmtp1 with SMTP; 7 Feb 2006 08:06:43 -0800 9, 20 -- Message-Id: {6.2.3.4.2.20060207080513.02057d00-at-mail.calweb.com} 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 20 -- Date: Tue, 07 Feb 2006 08:06:43 -0800 9, 20 -- To: RANGETS-at-AOL.COM 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] viaWWW: NEED SCHEMATICS LEO 440 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200602070430.k174UXNn021696-at-ns.microscopy.com} 9, 20 -- References: {200602070430.k174UXNn021696-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
what you recommend in your note is the logical solution. actually, i had meant to try the same change for my last embedment of monolayers to correct for the hardness/brittleness. unfortunately, i got distracted and forgot. i suspect that to get the medium hardness you may need 8-9 ml of the ERL component.
also, i used to use 0.4gm DMAE but changed that to .3gm to give a longer pot life. also, higher catalyst concentration could have an effect on viscosity and penetration.
paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 10, 21 -- From paul_hazelton-at-umanitoba.ca Tue Feb 7 15:19:25 2006 10, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 10, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k17LJPwp019538 10, 21 -- for {microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 15:19:25 -0600 10, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 10, 21 -- (authenticated bits=0) 10, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id k17LJFkD028260; 10, 21 -- Tue, 7 Feb 2006 15:19:24 -0600 (CST) 10, 21 -- Message-ID: {43E90ED0.7030901-at-umanitoba.ca} 10, 21 -- Date: Tue, 07 Feb 2006 15:19:12 -0600 10, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 10, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 10, 21 -- X-Accept-Language: en-us, en 10, 21 -- MIME-Version: 1.0 10, 21 -- To: DRK-at-SHCC.org 10, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 10, 21 -- Subject: Re: [Microscopy] RE: viaWWW: Spurr Resin 10, 21 -- References: {200602071558.k17Fw6YT022905-at-ns.microscopy.com} 10, 21 -- In-Reply-To: {200602071558.k17Fw6YT022905-at-ns.microscopy.com} 10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
An immediate opening exists for a skilled microscopist in Dow's Global Analytical Sciences Laboratory part of Dow's Corporate R&D function. We are seeking an individual with a passion for characterization science and a desire to both apply this science to complex industrial problems and to advance the technology. A Ph.D. in science is preferred, but an individual with a Masters degree and relevant work experience will also be considered. Working experience in electron microscopy is essential with skills in scanning and transmission electron microscopy, focused ion beam methods, energy dispersive x-ray analysis, electron energy loss spectroscopy, electron diffraction and microtomy preferred. A background in catalysis is a plus. Excellent written and oral communication skills (English) are essential with an ability to work in a globally diverse team environment. The position supports new product development activities working from a laboratory with a FEG TEM-STEM, 2 TEMs, a FIBSEM, EPMA, FEGSEM with cryotransfer system, a full complement of scanned probe and light microscopes, SAXS, XPS and TOF-SIMS. The position is located in Midland, Michigan. Applicants must have the ability to work in the USA.
Dow is a leader in science and technology, providing innovative chemical, plastic and agricultural products and services to many essential consumer markets. With annual sales of $40 billion, Dow serves customers in 175 countries and a wide range of markets that are vital to human progress: food, transportation, health and medicine, personal and home care, and building and construction, among others. Committed to the principles of sustainable development, Dow and its 43,000 employees seek to balance economic, environmental and social responsibilities. References to "Dow" or the "Company" mean The Dow Chemical Company and its consolidated subsidiaries unless otherwise expressly noted.
Please submit curriculum vitae and a list of references to Dr. John Blackson, Building 1897, The Dow Chemical Company, Midland, MI 48667.
Best Regards, Bill William A. Heeschen, Ph.D. Microscopy, Digital Imaging The Dow Chemical Company Midland, MI 48674 waheeschen-at-dow.com
==============================Original Headers============================== 7, 23 -- From WAHeeschen-at-dow.com Tue Feb 7 16:05:53 2006 7, 23 -- Received: from mail86.messagelabs.com (mail86.messagelabs.com [216.82.244.115]) 7, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k17M5q3V029468 7, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Feb 2006 16:05:52 -0600 7, 23 -- X-VirusChecked: Checked 7, 23 -- X-Env-Sender: WAHeeschen-at-dow.com 7, 23 -- X-Msg-Ref: server-4.tower-86.messagelabs.com!1139349950!29545829!1 7, 23 -- X-StarScan-Version: 5.5.9.1; banners=-,-,- 7, 23 -- X-Originating-IP: [204.136.184.23] 7, 23 -- Received: (qmail 26574 invoked from network); 7 Feb 2006 22:05:51 -0000 7, 23 -- Received: from mail6.dow.com (HELO txnte41.nam.dow.com) (204.136.184.23) 7, 23 -- by server-4.tower-86.messagelabs.com with SMTP; 7 Feb 2006 22:05:51 -0000 7, 23 -- Received: by TXNTE41.nam.dow.com with Internet Mail Service (5.5.2658.3) 7, 23 -- id {1A02KCZD} ; Tue, 7 Feb 2006 16:05:50 -0600 7, 23 -- Message-ID: {CC55EF96AD3142438EF779F71571702084A20F-at-USMDLMDOWX004.dow.com} 7, 23 -- From: "Heeschen, Bill (WA)" {WAHeeschen-at-dow.com} 7, 23 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 7, 23 -- Subject: Job Posting from The Dow Chemical Company 7, 23 -- Date: Tue, 7 Feb 2006 16:05:31 -0600 7, 23 -- Expiry-Date: Tue, 6 Feb 2007 23:00:00 -0600 7, 23 -- MIME-Version: 1.0 7, 23 -- X-Mailer: Internet Mail Service (5.5.2658.3) 7, 23 -- Content-Type: text/plain ==============================End of - Headers==============================
I just got off the phone with Stacy (the owner) at Electron Microscopy Sciences. I discussed my recent problems with Spurr's resin with her. She told me that one component, ERL 4206, is no longer maunfactured due to its high toxicity. It has been replaced with ERL 4221. She thinks that this is the cause of recent problems with Spurr embedments. She has discussed this with others (I did not ask who) and the consensus recommendations are to prolong dehydration in graded ethanols to 20 minutes each step, make the 1:1 prop. oxide:Spurr step 4 hours and make the first change of pure resin 6 hours or longer. Since longer times in solvents are known to extract cytoplasmic components (and I cannot imaging that a 100 micron Vibratome section of CNS needs 20 min. per change of graded ethanol) I am going back to Epon substitutes. Also, my tissues are in a second change of fresh resin on a rotator overnight and then spend at least 1 hour under vacuum, I can't see how infiltration could be a problem. I have used both the soft and firm mixtures with different tissues and had inconsistant block textures and infiltration problems with both. I don't have these problems with an Araldite kit I bought at the same time. I can't risk knock-out mice in long-term treatment/recovery experiments to reagents I cannot be sure of.
Geoff
-- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 6, 29 -- From mcauliff-at-umdnj.edu Wed Feb 8 15:25:23 2006 6, 29 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k18LPNGG000454 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Feb 2006 15:25:23 -0600 6, 29 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id BC9CB4BE54 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Feb 2006 16:25:22 -0500 (EST) 6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 6, 29 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 063404BE5C 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Wed, 8 Feb 2006 16:25:22 -0500 (EST) 6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 6, 29 -- id {0IUD00E01ZO0X6-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 6, 29 -- for microscopy-at-msa.microscopy.com; Wed, 08 Feb 2006 16:25:21 -0500 (EST) 6, 29 -- Received: from [127.0.0.1] ([10.138.2.240]) 6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 6, 29 -- 2004)) with ESMTP id {0IUD00DAGZSNRH-at-Polaris.umdnj.edu} for 6, 29 -- microscopy-at-msa.microscopy.com; Wed, 08 Feb 2006 16:02:47 -0500 (EST) 6, 29 -- Date: Wed, 08 Feb 2006 16:02:08 -0500 6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 6, 29 -- Subject: Spurr's resin update 6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com} 6, 29 -- Message-id: {43EA5C50.6070607-at-umdnj.edu} 6, 29 -- MIME-version: 1.0 6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 6, 29 -- Content-transfer-encoding: 7BIT 6, 29 -- X-Accept-Language: en-us, en 6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 29 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
I will be experimenting with alternative scintillators for our Hitachi S4800 STEM unit. As I was advised to no touch the scintillator in the microscope, I hope someone will be able to offer measurements for the original S4800 scintillator.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
We are just starting an experiment with en-bloc staining of tissue in uranyl acetate in 70% ethanol. It seems to me that since the blocks are larger than thin sections, they probably take up more radioactive material- so should we take any precautions when handling the blocks after the resin has cured? (i.e. store the blocks in separate special containers, wear gloves when sectioning or are there any special cleaning procedures for the knife?).
thank you for sharing your knowledge
Gerd
Dr. Gerd Leitinger
Institut für Zellbiologie, Histologie und Embryologie Medizinische Universität Graz Harrachgasse 21 A-8010 Graz Austria
I am seeking to buy NanoScope AFM equipment, such as MultiMode or Dimension, to use with my Nanoscope IIIA controller. If you have anything to sell, please contact me offline.
regards, Don Chernoff ================================== Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.]
==============================Original Headers============================== 3, 21 -- From donc-at-asmicro.com Thu Feb 9 06:56:12 2006 3, 21 -- Received: from smtp112.sbc.mail.re2.yahoo.com (smtp112.sbc.mail.re2.yahoo.com [68.142.229.93]) 3, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k19CuBAg016578 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 06:56:11 -0600 3, 21 -- Received: (qmail 34978 invoked from network); 9 Feb 2006 12:56:11 -0000 3, 21 -- Received: from unknown (HELO ASM11) (asmicro-at-sbcglobal.net-at-68.57.205.80 with login) 3, 21 -- by smtp112.sbc.mail.re2.yahoo.com with SMTP; 9 Feb 2006 12:56:11 -0000 3, 21 -- Message-ID: {006801c62d77$ebd28b80$c901a8c0-at-ASM11} 3, 21 -- Reply-To: "Don Chernoff at ASM" {donc-at-asmicro.com} 3, 21 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 3, 21 -- To: "Microscopy List" {microscopy-at-microscopy.com} 3, 21 -- Subject: AFM equipment wanted 3, 21 -- Date: Thu, 9 Feb 2006 07:54:03 -0500 3, 21 -- MIME-Version: 1.0 3, 21 -- Content-Type: text/plain; 3, 21 -- charset="iso-8859-1" 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-Priority: 3 3, 21 -- X-MSMail-Priority: Normal 3, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1506 3, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 ==============================End of - Headers==============================
We are looking to purchase a second-hand Gatan 622SC camera (200kV-compatible) in decent working condition to install on our JEOL 2100 TEM. We are hoping to find one with a working intensifier and an intact scintillator, although any problems are possibly acceptable. We would be able to pay for it using a University of Maryland PO. The amount of money might allow you to upgrade your TEM to a digital CCD camera from AMT.
Please do not reply to the listserver. Direct any inquiries or offers directly to me at the email address below.
Many thanks,
-John
-- John Cumings cumings-at-umd.edu Assistant Professor Department of Materials Science and Engineering University of Maryland College Park, MD 20742-2115
office (301) 405-0789 (1246 Kim Building) fax (301) 314-8164 --
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We have a few users study bacterial pili using negative staining with PTA (pH 6.5). We have got some pretty nice images. But often time, we could not find pili even on the positive control. When we did see pili, they were not on all bacteria in the same sample. Is this a common phenomenon? Do bacteria lose their pili easily when the external condition is not favorable? if that is the case, what should be done to minimize the loss.
Thank you in advance.
Hong Emory EM
==============================Original Headers============================== 5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600 5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1]) 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k19G6qIx024009 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu} 5, 22 -- Content-Type: text/plain; 5, 22 -- charset=ISO-8859-1; 5, 22 -- format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Hong Yi {hyi-at-emory.edu} 5, 22 -- Subject: Bacterial Pili 5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500 5, 22 -- X-Mailer: Apple Mail (2.622) 5, 22 -- X-imss-version: 2.037 5, 22 -- X-imss-result: Passed 5, 22 -- X-imss-approveListMatch: *-at-emory.edu 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19G6qLb005145 ==============================End of - Headers==============================
We often have this same problem. At least in our lab, flagella and pili seem to hide when we're trying to find them and show up when we don't care if we see them or not.
As a rule, they seem to be easily detached by rough handling, including centrifuging, and maybe by the staining process itself, since we often find them isolated from the bacteria on the grid.
One group of researchers gets around this problem by growing the bacteria on carbon coated grids and staining them in situ. Check JOURNAL OF BACTERIOLOGY, Feb. 2005, p. 1173-1181 for images. For preparation details this article references Brown et al, 2001. Immunocytochemical localization of HrpA and HrpZ supports a role for the Hrp pilus in the transfer of effector proteins from Pseudomonassyringae pv. tomato across the host plant cell wall. Mol. Plant-Microbe Interact. 14:394-404. I have not yet read the last article, so I can't comment on it.
Good luck.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
-----Original Message----- X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu] Sent: Thursday, February 09, 2006 10:08 AM To: Tindall, Randy D.
Dear Microscopists:
We have a few users study bacterial pili using negative staining with PTA (pH 6.5). We have got some pretty nice images. But often time, we could not find pili even on the positive control. When we did see pili, they were not on all bacteria in the same sample. Is this a common phenomenon? Do bacteria lose their pili easily when the external condition is not favorable? if that is the case, what should be done to minimize the loss.
Thank you in advance.
Hong Emory EM
==============================Original Headers============================== 5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600 5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1]) 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k19G6qIx024009 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu} 5, 22 -- Content-Type: text/plain; 5, 22 -- charset=ISO-8859-1; 5, 22 -- format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Hong Yi {hyi-at-emory.edu} 5, 22 -- Subject: Bacterial Pili 5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500 5, 22 -- X-Mailer: Apple Mail (2.622) 5, 22 -- X-imss-version: 2.037 5, 22 -- X-imss-result: Passed 5, 22 -- X-imss-approveListMatch: *-at-emory.edu 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19G6qLb005145 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 24 -- From TindallR-at-missouri.edu Thu Feb 9 10:24:23 2006 20, 24 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 20, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19GOMnC014637 20, 24 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:24:22 -0600 20, 24 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 20, 24 -- Thu, 9 Feb 2006 10:24:22 -0600 20, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 24 -- Content-class: urn:content-classes:message 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="iso-8859-1" 20, 24 -- Subject: RE: [Microscopy] Bacterial Pili 20, 24 -- Date: Thu, 9 Feb 2006 10:24:21 -0600 20, 24 -- Message-ID: {BA876152E8653240BE8572E897083EE701849D33-at-UM-EMAIL09.um.umsystem.edu} 20, 24 -- X-MS-Has-Attach: 20, 24 -- X-MS-TNEF-Correlator: 20, 24 -- Thread-Topic: [Microscopy] Bacterial Pili 20, 24 -- thread-index: AcYtkwuBClshBOD6QsCkENhoqgFICQAAJ20Q 20, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 20, 24 -- To: {hyi-at-emory.edu} 20, 24 -- Cc: {microscopy-at-microscopy.com} 20, 24 -- X-OriginalArrivalTime: 09 Feb 2006 16:24:22.0502 (UTC) FILETIME=[49041860:01C62D95] 20, 24 -- Content-Transfer-Encoding: 8bit 20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19GOMnC014637 ==============================End of - Headers==============================
Rough handling may contribute to loss of pili, however the PTA itself is probably the culprit. Try changing the pH either up or down. Way back when, I did a study with rotavirus and various negative stains. Bottom line is that the length of time of staining, pH and concentration all contributed to the destruction (preservation) of the viral particles. If I remember correctly, the length of time was the most important factor. Also try using either uranyl acetate or ammonium molybdate as the negative stain.
Best of luck,
Ed
Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu -----Original Message----- X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu] Sent: Thursday, February 09, 2006 11:29 AM To: Edward Calomeni
Hong,
We often have this same problem. At least in our lab, flagella and pili seem to hide when we're trying to find them and show up when we don't care if we see them or not.
As a rule, they seem to be easily detached by rough handling, including centrifuging, and maybe by the staining process itself, since we often find them isolated from the bacteria on the grid.
One group of researchers gets around this problem by growing the bacteria on carbon coated grids and staining them in situ. Check JOURNAL OF BACTERIOLOGY, Feb. 2005, p. 1173-1181 for images. For preparation details this article references Brown et al, 2001. Immunocytochemical localization of HrpA and HrpZ supports a role for the Hrp pilus in the transfer of effector proteins from Pseudomonassyringae pv. tomato across the host plant cell wall. Mol. Plant-Microbe Interact. 14:394-404. I have not yet read the last article, so I can't comment on it.
Good luck.
Randy
Randy Tindall EM Specialist Electron Microscopy Core Facility---We Do Small Well! W122 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu
-----Original Message----- X-from: hyi-at-emory.edu [mailto:hyi-at-emory.edu] Sent: Thursday, February 09, 2006 10:08 AM To: Tindall, Randy D.
Dear Microscopists:
We have a few users study bacterial pili using negative staining with PTA (pH 6.5). We have got some pretty nice images. But often time, we could not find pili even on the positive control. When we did see pili, they were not on all bacteria in the same sample. Is this a common phenomenon? Do bacteria lose their pili easily when the external condition is not favorable? if that is the case, what should be done to minimize the loss.
Thank you in advance.
Hong Emory EM
==============================Original Headers============================== 5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600 5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1]) 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k19G6qIx024009 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu} 5, 22 -- Content-Type: text/plain; 5, 22 -- charset=ISO-8859-1; 5, 22 -- format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Hong Yi {hyi-at-emory.edu} 5, 22 -- Subject: Bacterial Pili 5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500 5, 22 -- X-Mailer: Apple Mail (2.622) 5, 22 -- X-imss-version: 2.037 5, 22 -- X-imss-result: Passed 5, 22 -- X-imss-approveListMatch: *-at-emory.edu 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19G6qLb005145 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 24 -- From TindallR-at-missouri.edu Thu Feb 9 10:24:23 2006 20, 24 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 20, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19GOMnC014637 20, 24 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:24:22 -0600 20, 24 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 20, 24 -- Thu, 9 Feb 2006 10:24:22 -0600 20, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 24 -- Content-class: urn:content-classes:message 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-Type: text/plain; 20, 24 -- charset="iso-8859-1" 20, 24 -- Subject: RE: [Microscopy] Bacterial Pili 20, 24 -- Date: Thu, 9 Feb 2006 10:24:21 -0600 20, 24 -- Message-ID: {BA876152E8653240BE8572E897083EE701849D33-at-UM-EMAIL09.um.umsystem.edu} 20, 24 -- X-MS-Has-Attach: 20, 24 -- X-MS-TNEF-Correlator: 20, 24 -- Thread-Topic: [Microscopy] Bacterial Pili 20, 24 -- thread-index: AcYtkwuBClshBOD6QsCkENhoqgFICQAAJ20Q 20, 24 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 20, 24 -- To: {hyi-at-emory.edu} 20, 24 -- Cc: {microscopy-at-microscopy.com} 20, 24 -- X-OriginalArrivalTime: 09 Feb 2006 16:24:22.0502 (UTC) FILETIME=[49041860:01C62D95] 20, 24 -- Content-Transfer-Encoding: 8bit 20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19GOMnC014637 ==============================End of - Headers==============================
==============================Original Headers============================== 31, 31 -- From Edward.Calomeni-at-osumc.edu Thu Feb 9 11:18:04 2006 31, 31 -- Received: from pluto.osumc.edu (pluto.osumc.edu [140.254.120.27]) 31, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19HI3nX024586 31, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:18:03 -0600 31, 31 -- Received: from localhost (unknown [127.0.0.1]) 31, 31 -- by pfeg02.osumc.edu (Postfix) with ESMTP id 01EE2E22B 31, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 12:18:04 -0500 (EST) 31, 31 -- Received: from pfeg02.osumc.edu ([127.0.0.1]) 31, 31 -- by localhost (pfeg02 [127.0.0.1]) (amavisd-new, port 10024) with LMTP 31, 31 -- id 04255-01-25 for {Microscopy-at-microscopy.com} ; 31, 31 -- Thu, 9 Feb 2006 17:18:03 +0000 (GMT) 31, 31 -- Received: from msxc01.OSUMC.EDU (unknown [10.127.29.33]) 31, 31 -- by pfeg02.osumc.edu (Postfix) with ESMTP id D2A8BE03F 31, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 12:18:03 -0500 (EST) 31, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 31, 31 -- Content-class: urn:content-classes:message 31, 31 -- MIME-Version: 1.0 31, 31 -- Content-Type: text/plain; 31, 31 -- charset="iso-8859-1" 31, 31 -- Subject: RE: Bacterial Pili 31, 31 -- Date: Thu, 9 Feb 2006 12:18:03 -0500 31, 31 -- Message-ID: {7A541592F9A53A4E86E7A3D5C4C7F68C27F9F4-at-MSXC01} 31, 31 -- X-MS-Has-Attach: 31, 31 -- X-MS-TNEF-Correlator: 31, 31 -- Thread-Topic: RE: Bacterial Pili 31, 31 -- Thread-Index: AcYtleiHrKzoLX0fRmSx0dkDmP6AHwABibyw 31, 31 -- From: "Edward Calomeni" {Edward.Calomeni-at-osumc.edu} 31, 31 -- To: {Microscopy-at-microscopy.com} 31, 31 -- X-Virus-Scanned: amavisd-new at osumc.edu 31, 31 -- Content-Transfer-Encoding: 8bit 31, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k19HI3nX024586 ==============================End of - Headers==============================
On Feb 9, 2006, at 4:45 AM, gerd.leitinger-at-meduni-graz.at wrote:
} We are just starting an experiment with en-bloc staining of tissue in } uranyl } acetate in 70% ethanol. } It seems to me that since the blocks are larger than thin sections, } they } probably take up more radioactive material- so should we take any } precautions when handling the blocks after the resin has cured? (i.e. } store } the blocks in separate special containers, wear gloves when sectioning } or } are there any special cleaning procedures for the knife?). } } thank you for sharing your knowledge } Dear Gerd, There is still a very small amount of radioactive material in the block; furthermore, uranium has a very long half-life, thus very small activity, and the alpha particles it emits have a short range, so any decays except those nearer the surface of the block than the thickness of the dead layer of your skin will not result in much radiation leaving the block. You can easily measure this with an ionization chamber (to detect the x- and gamma-radiation that make up most of the escaping radiation). That said, the laws regarding the handling of radioactive materials--even those with very little activity--vary from country to country, locale to locale, and sometimes for different institutions within a particular locale, so your safety office may mandate special procedures for storage and handling. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Thu Feb 9 12:48:19 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19ImIdP002791 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Feb 2006 12:48:19 -0600 4, 22 -- Received: from localhost (water-dog [192.168.1.26]) 4, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 33AB23422E 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Feb 2006 10:48:18 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 6844F33A1E 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Thu, 9 Feb 2006 10:48:17 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200602091245.k19Cj91Q007324-at-ns.microscopy.com} 4, 22 -- References: {200602091245.k19Cj91Q007324-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {9fac5de4ab7271490d0c8faa23e1db2b-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] EM: precautions when en bloc staining 4, 22 -- Date: Thu, 9 Feb 2006 10:55:56 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
Dear Hong, I have been using 2% Uranyl acetate to visualize bacterial pili.
I have been using 2% Uranyl acetate to visualize pili. I have had to play with the time of staining and rinsing in order to get a good contrast to visualize the pili on different bacterial genera. I have noticed that not all bacteria show the same distribution of pili. I have also noticed that the way you grow the bacteria also affect visualization of pili. For our bacteria I have noticed that I get far better results when I grow them up in stationary cultures without shaking them.
What I have been seeing it the bacteria behave differently when in aggregation versus solitude. This is more so owing to the several differnt kinds of pili each bacterial strain is capable of forming. If the bacteria you are working with produces different types of pili then you will see differences between the bacterial cells visualized on the same grid.
I have had the same strain of bacteria producing really small pili which were visualized only at a very high mag. Hope that was helpful regards, Vinod Graduate Student Dept Of Biology New Mexico State University On 2/9/06, hyi-at-emory.edu {hyi-at-emory.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Microscopists: } } } We have a few users study bacterial pili using negative staining with } PTA (pH 6.5). We have got some pretty nice images. But often time, we } could not find pili even on the positive control. When we did see } pili, they were not on all bacteria in the same sample. Is this a } common phenomenon? Do bacteria lose their pili easily when the external } condition is not favorable? if that is the case, what should be done to } minimize the loss. } } Thank you in advance. } } Hong } Emory EM } } } ==============================Original Headers============================== } 5, 22 -- From hyi-at-emory.edu Thu Feb 9 10:06:53 2006 } 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu } [170.140.8.222]) } 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19G6qLb005145 } 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 10:06:53 -0600 } 5, 22 -- Received: from [170.140.232.204] (localhost [127.0.0.1]) } 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id } k19G6qIx024009 } 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 11:06:52 -0500 } (EST) } 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) } 5, 22 -- Message-Id: {56be70af163d5629d409dc9e17804801-at-emory.edu} } 5, 22 -- Content-Type: text/plain; } 5, 22 -- charset=ISO-8859-1; } 5, 22 -- format=flowed } 5, 22 -- To: Microscopy-at-microscopy.com } 5, 22 -- From: Hong Yi {hyi-at-emory.edu} } 5, 22 -- Subject: Bacterial Pili } 5, 22 -- Date: Thu, 9 Feb 2006 11:06:51 -0500 } 5, 22 -- X-Mailer: Apple Mail (2.622) } 5, 22 -- X-imss-version: 2.037 } 5, 22 -- X-imss-result: Passed } 5, 22 -- X-imss-approveListMatch: *-at-emory.edu } 5, 22 -- Content-Transfer-Encoding: 8bit } 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id k19G6qLb005145 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 25 -- From nairvinods-at-gmail.com Thu Feb 9 13:08:22 2006 5, 25 -- Received: from wproxy.gmail.com (wproxy.gmail.com [64.233.184.202]) 5, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19J8Mrd012527 5, 25 -- for {microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 13:08:22 -0600 5, 25 -- Received: by wproxy.gmail.com with SMTP id i6so371281wra 5, 25 -- for {microscopy-at-microscopy.com} ; Thu, 09 Feb 2006 11:08:22 -0800 (PST) 5, 25 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 25 -- s=beta; d=gmail.com; 5, 25 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 5, 25 -- b=EaYibLaG48bwxRHn2D1rKg1YmKGyCmDnLAqNF+/f9UuSlcSOnBwQBCRPkBMXnmuuxJwTWlUo0r2HcibDDb78jLOk2qjdWIZ4b+Fwteoxowx/jA8BP9NFOLKwkpPzMMYrvgo4Ba23mGe3Ujr2MYVW72FvurUNj517iGySHrdgce8= 5, 25 -- Received: by 10.65.180.18 with SMTP id h18mr161301qbp; 5, 25 -- Thu, 09 Feb 2006 11:08:21 -0800 (PST) 5, 25 -- Received: by 10.64.203.13 with HTTP; Thu, 9 Feb 2006 11:08:21 -0800 (PST) 5, 25 -- Message-ID: {ea42a3900602091108occ4907aw88fdbc39652af5a0-at-mail.gmail.com} 5, 25 -- Date: Thu, 9 Feb 2006 12:08:21 -0700 5, 25 -- From: Vinod Nair {nairvinods-at-gmail.com} 5, 25 -- To: microscopy-at-microscopy.com 5, 25 -- Subject: Re: Bacterial Pili 5, 25 -- In-Reply-To: {200602091612.k19GC6Bt012799-at-ns.microscopy.com} 5, 25 -- MIME-Version: 1.0 5, 25 -- Content-Type: text/plain; charset=UTF-8 5, 25 -- Content-Disposition: inline 5, 25 -- References: {200602091612.k19GC6Bt012799-at-ns.microscopy.com} 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id k19J8Mrd012527 ==============================End of - Headers==============================
I'm compiling a list of chemical stains/etches for delineating N+ and P+ source/drain implants/diffusions on semiconductor die cross sections. Any suggestions?
==============================Original Headers============================== 3, 22 -- From icmicroanalysis-at-cox.net Thu Feb 9 13:09:32 2006 3, 22 -- Received: from fed1rmmtao03.cox.net (fed1rmmtao03.cox.net [68.230.241.36]) 3, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k19J9WI4014727 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 13:09:32 -0600 3, 22 -- Received: from officehp1 ([68.98.17.129]) by fed1rmmtao03.cox.net 3, 22 -- (InterMail vM.6.01.05.02 201-2131-123-102-20050715) with SMTP 3, 22 -- id {20060209190811.LEDO20875.fed1rmmtao03.cox.net-at-officehp1} 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2006 14:08:11 -0500 3, 22 -- From: "IC Microanalysis" {icmicroanalysis-at-cox.net} 3, 22 -- To: {Microscopy-at-microscopy.com} 3, 22 -- Subject: Stains/etches for delineating source/drains diffusions on semiconductor die cross sections 3, 22 -- Date: Thu, 9 Feb 2006 12:09:30 -0700 3, 22 -- Message-ID: {IKEHJCMBLNGNPJBGCPGJIEKACCAA.icmicroanalysis-at-cox.net} 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: text/plain; 3, 22 -- charset="iso-8859-1" 3, 22 -- Content-Transfer-Encoding: 7bit 3, 22 -- X-Priority: 3 (Normal) 3, 22 -- X-MSMail-Priority: Normal 3, 22 -- X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2910.0) 3, 22 -- Importance: Normal 3, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 ==============================End of - Headers==============================
I put together a summary of the responses to my query on ESEM gas choices. People responded that they had experimented with Nitrogen, Nitrous Oxide, Oxygen, and Helium. Opinions were mixed on the performance of various gases (see below), but in general I feel comfortable trying Nitrogen in the near future. I received warnings that Helium can damage photomultipliers and Argon can damage ion pumps. Because of safety issues, we will most likely avoid oxygen and nitrous oxide for the time being.
FEI also contacted me and reminded that before experimenting with various gases in the ESEM, I should check with them to make sure that warranties are not voided by using any particular gas. If a gas could damage your system or components, the manufacturer should be able to tell you before you damage your system. Always check with the manufacturer before changing major system parameters.
My detector issue drew a number of separate questions and queries, and we are working separately with the manufacturers on this. We did learn that we can collect spectra with the SDD crystal at room temperature. This eliminates the potential for vapor condensation on the crystal surface. There is some peak broadening and a slightly higher background when we do this, but it does provide us with bulk data and may prove to be our final solution.
I also learned that many bulk gases also contain amounts of moisture that might condense under variable pressure conditions. Ultra high purity, low moisture gas, is sometimes considerably more expensive than bulk lab gas. I have to check with my vendor to find out what kind of moisture my tank might contain before hooking it to the ESEM.
Thanks again for everyone's help on this. The list has proven itself invaluable again.
Karl
----- "I have used water vapor, N2 and Argon. None of them gave as good a signal (image) as the water vapor did.... The Ionization of the other gasses is apparently not that good and give no nice images" ----- "I have been using dry nitrogen with my variable pressure instrument. We vent the system with the same gas. My EDS system seems to work well but have not run extensive tests to determine what effects the gas has other than the beam scattering issues." ----- " A disadvantage to using helium is your PMT's could be damaged, if it is released into the room. Also, another gas you could be careful with is argon, as it is a ion-pump poison (if released into a ion-pumped system). Nitrogen sounds friendly to me" ----- "In my experience, if you can't use oxygen the next best gas to use is argon - easily obtainable and provides good imaging conditions. Air and nitrogen should be avoided, since the nitrogen breaks down at too low a voltage and you can't get much signal. Some European groups looked at a wider range of imaging gases and nitrous oxide I believe did very well, although I have not tried it. I have tried oxygen and it works well, but tends to ruin the pump oil fairly quickly." ----- "The Quanta 200 uses a W filament. Unless it is a SFEG. If there are no ion pumps, you should be able to use Nitrogen or He. If there is an ion pump, do not use He. It will kill the pump.
I found that for VP (20-120Pa) that N2 works fine and is better than air (less vacuum issues). So, for ESEM, I would think that N2 ought to be the gas of choice as well." ----- " We routinely use He in our Hitachi VP-SEM. It has a tungsten gun and no ion pumps, so there is no problem with fouling those pumps as Gary Gaugler mentioned.
If you consider that He is a monoatomic species with a weight of 4 and that nitrogen is diatomic with a weight of 28, your gut can tell you that He will scatter less than N2 or room air. That is our experience and we can see the effect in iamges.
There is no effect on the x-ray signals from He that we have ever seen. He x-rays are below our low-energy discriminator. I suppose one could see N or O x-rays due to the gas, but I suppose their contribution is much less than from the sample. You could arrange a test to evaluate that, say you collected spectra from a beryllium or boron sample using high vacuum, helium, and nitrogen." ----- Original Post: --- ----
I am interested in hearing what type of gases people are using in their ESEM applications. We recently learned our silicon drift detector is incompatible with water vapor, and we apparently cannot complete any x-ray microanalysis while working in standard ESEM or VP modes (I was pretty surprised to learn this). We are thinking that using a dry gas such as nitrogen or helium will allow us to work in ESEM modes and use our x-ray system as well, as there is no potential for moisture condensing on the crystal surface.
Our ESEM is a Quanta 200, and it is not equipped with the peltier stage, so we mainly use the environmental modes to alleviate charging in uncoated samples. The x-ray system was purchased with the microscope about four years ago. I would rather not discuss the name of the x-ray vendor on the list, as we have a significant investment in this detector and do not foresee finding the money to purchase a new one soon.
Our first concern is the impact of the gas on spectra. We can coat samples and run them in high vacuum mode, but customers like spectra that represent the sample and not the coating material. We periodically have samples that cannot be coated, so ESEM becomes critical for our imaging needs. If the gas is going to have a dramatic impact on signal, are we better off coating and running high vacuum?
Is there a best choice for chamber gas? Our service engineer recommends nitrogen as having benefits for keeping the system clean. We can set it up fairly quickly, and I have a spare tank and regulator. I have heard of people using helium and believe that I read somewhere that there was some advantage to using helium.
I am still trying to get information from the vendor on whether this will allow us to use the x-ray and ESEM simultaneously, or whether we have options with different collimators or windows. Their responses have been pretty slow coming. _____
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 18, 23 -- From hagglundk1-at-nku.edu Fri Feb 10 10:10:59 2006 18, 23 -- Received: from mailfe2.nku.edu (exchange.nku.edu [192.122.237.68]) 18, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AGAxYh024896 18, 23 -- for {microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 10:10:59 -0600 18, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 18, 23 -- Fri, 10 Feb 2006 11:10:59 -0500 18, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 18, 23 -- Content-class: urn:content-classes:message 18, 23 -- MIME-Version: 1.0 18, 23 -- Content-Type: text/plain; 18, 23 -- charset="us-ascii" 18, 23 -- Subject: Follow up ESEM gas choices 18, 23 -- Date: Fri, 10 Feb 2006 11:10:56 -0500 18, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F358647-at-mailfac1.hh.nku.edu} 18, 23 -- X-MS-Has-Attach: 18, 23 -- X-MS-TNEF-Correlator: 18, 23 -- Thread-Topic: Follow up ESEM gas choices 18, 23 -- Thread-Index: AcYuXJL+d7H9dmpuRdS+OZyc5Rw51w== 18, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 18, 23 -- To: {microscopy-at-microscopy.com} 18, 23 -- X-OriginalArrivalTime: 10 Feb 2006 16:10:59.0208 (UTC) FILETIME=[94A0E880:01C62E5C] 18, 23 -- Content-Transfer-Encoding: 8bit 18, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1AGAxYh024896 ==============================End of - Headers==============================
Daniel, If you contact Gene Taylor at M. E. Taylor Engineering
Phone 301-774-6246
Fax 301-774-6711
www.semsupplies.com
I'm sure he can give you what you need. Scintillators have been his specialty for decades. Also, many of the other EM supply companies should be able to provide what you're looking for.
Disclaimer: A number of years ago when my business was larger, I was a distributor of Taylor supplies and have many happy customers.
Ken Converse Owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Daniel.Salamon-at-nrc-cnrc.gc.ca [mailto:Daniel.Salamon-at-nrc-cnrc.gc.ca] Sent: Wednesday, February 08, 2006 4:35 PM To: kenconverse-at-qualityimages.biz
Hi everyone,
I will be experimenting with alternative scintillators for our Hitachi S4800 STEM unit. As I was advised to no touch the scintillator in the microscope, I hope someone will be able to offer measurements for the original S4800 scintillator.
Thanks, Daniel Salamon Technical Officer, Electron Microscopy
National Institute for Nanotechnology, NRC W6-017A ECERF Bldg, 9107-116 Street Edmonton, AB. T6G 2V4
==============================Original Headers============================== 5, 23 -- From Daniel.Salamon-at-nrc-cnrc.gc.ca Wed Feb 8 15:33:09 2006 5, 23 -- Received: from nrccenexf2.nrc.ca (nrccenexf2.nrc.ca [132.246.15.83]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k18LX8mG009977 5, 23 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2006 15:33:08 -0600 5, 23 -- Received: from nrccenexb2.nrc.ca ([132.246.15.98]) by nrccenexf2.nrc.ca with Microsoft SMTPSVC(6.0.3790.1830); 5, 23 -- Wed, 8 Feb 2006 16:33:07 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: S4800 STEM scintillator size 5, 23 -- Date: Wed, 8 Feb 2006 16:32:19 -0500 5, 23 -- Message-ID: {923687253229634E96E808B8D3124C83557517-at-nrccenexb2.nrc.ca} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: S4800 STEM scintillator size 5, 23 -- Thread-Index: AcYs9yOtLSo8zGW/Rh2wOcOIwp9aqA== 5, 23 -- From: "Salamon, Daniel" {Daniel.Salamon-at-nrc-cnrc.gc.ca} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 08 Feb 2006 21:33:07.0447 (UTC) FILETIME=[4054A070:01C62CF7] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k18LX8mG009977 ==============================End of - Headers==============================
___________________________________________________________ $0 Web Hosting with up to 200MB web space, 1000 MB Transfer 10 Personalized POP and Web E-mail Accounts, and much more. Signup at www.doteasy.com
==============================Original Headers============================== 26, 24 -- From kenconverse-at-qualityimages.biz Fri Feb 10 10:20:24 2006 26, 24 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 26, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AGKLGN000476 26, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Fri, 10 Feb 2006 10:20:22 -0600 26, 24 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 26, 24 -- (SMTPD32-8.05) id ADB831560154; Fri, 10 Feb 2006 08:22:16 -0800 26, 24 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 26, 24 -- To: {Daniel.Salamon-at-nrc-cnrc.gc.ca} , 26, 24 -- "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 26, 24 -- Subject: RE: [Microscopy] S4800 STEM scintillator size 26, 24 -- Date: Fri, 10 Feb 2006 07:20:44 -0500 26, 24 -- Message-ID: {003b01c62e3c$746b43a0$6501a8c0-at-Ken} 26, 24 -- MIME-Version: 1.0 26, 24 -- Content-Type: text/plain; 26, 24 -- charset="us-ascii" 26, 24 -- X-Priority: 3 (Normal) 26, 24 -- X-MSMail-Priority: Normal 26, 24 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 26, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 26, 24 -- Importance: Normal 26, 24 -- In-Reply-To: {200602082135.k18LZD0D015228-at-ns.microscopy.com} 26, 24 -- X-IMSTrailer: __IMail_7__ 26, 24 -- Content-Transfer-Encoding: 8bit 26, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1AGKLGN000476 ==============================End of - Headers==============================
I am posting the following ad for Dr. Jingyue Liu at Monsanto. Please contact Dr. Liu for further information, email address at the bottom of the request.
Lou Ross
Two Contract Researchers Are Needed in the Catalyst Characterization Group of Monsanto Company
Job Location: Creve Coeur Campus, St. Louis, Missouri 63167
The following two openings (one year contract researchers) are immediately available:
1) Research associate for TEM sample preparation. Required skills: extensive experience and expertise in ultramicrotoming thin sections of catalyst powders or other nanophase materials for transmission electron microscopy observation. This job requires strong hands-on skills and new method development for unique samples. Experience with sample preparation equipments such as high vacuum carbon coating, embedding, fixing and staining biological tissues, and maintenance of sample preparation facility is highly desirable. Experience in operating SEM instruments is a plus.
2) Research associate for catalyst preparation, treatment and characterization. Required skills: extensive experience in preparing model and practical catalysts or nanoparticles and TEM characterization of such samples. Experience in catalyst treatment and testing is a plus. Strong hands-on skills and demonstrated capability of designing complex experiments are required for this job.
Both jobs require extensive hands-on experiences in research labs. The following competencies are required: innovation in solving challenging problems; good communication and interpersonal skills; teamwork skills and results orientation.
Since Monsanto does not directly hire contract researchers, the selected candidates will work for a contract agency. Interested parties please send your resume and application letter to: Jingyue.liu-at-monsanto.com.
-- Senior Electron Microscope Specialist Electron Microscopy Core Facility W136 Veterinary Medicine University of Missouri Columbia, MO 65211-5120 (573) 882-4777, fax 884=2227 email: rosslm-at-missouri.edu http://www.emc.missouri.edu
==============================Original Headers============================== 11, 17 -- From RossLM-at-missouri.edu Fri Feb 10 11:55:40 2006 11, 17 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 11, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AHtd3Q012471 11, 17 -- for {microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 11:55:39 -0600 11, 17 -- Received: from um-exproto9.um.umsystem.edu ([207.160.151.148]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 11, 17 -- Fri, 10 Feb 2006 11:55:39 -0600 11, 17 -- Received: from [128.206.78.231] ([128.206.78.231]) by um-exproto9.um.umsystem.edu over TLS secured channel with Microsoft SMTPSVC(6.0.3790.1830); 11, 17 -- Fri, 10 Feb 2006 11:55:39 -0600 11, 17 -- Mime-Version: 1.0 11, 17 -- X-Sender: RossLM-at-pop.missouri.edu 11, 17 -- Message-Id: {p05200f30c0128247bb75-at-[128.206.78.231]} 11, 17 -- Date: Fri, 10 Feb 2006 11:55:37 -0600 11, 17 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 11, 17 -- From: Lou Ross {RossLM-at-missouri.edu} 11, 17 -- Subject: 2 contract positions at Monsanto in St. Louis 11, 17 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 11, 17 -- X-OriginalArrivalTime: 10 Feb 2006 17:55:39.0395 (UTC) FILETIME=[33E96530:01C62E6B] ==============================End of - Headers==============================
I am comtemplating broadening a mainly TEM undergrad course (with a lab) to include some scanning probe techniques. Does anyone know of reasonable texts which have some coverage?
----------------------------------------------- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60201, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L - marks -at- northwestern . edu http://www.numis.northwestern.edu -----------------------------------------------
==============================Original Headers============================== 4, 16 -- From ldmm-at-risc4.numis.northwestern.edu Fri Feb 10 12:25:19 2006 4, 16 -- Received: from risc4.numis.northwestern.edu (risc4.numis.northwestern.edu [129.105.122.70]) 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1AIPJKL022849 4, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 12:25:19 -0600 4, 16 -- Received: from localhost (ldmm-at-localhost) 4, 16 -- by risc4.numis.northwestern.edu (8.9.3 (PHNE_28760_binary)/8.9.3) with ESMTP id MAA24082 4, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Feb 2006 12:25:14 -0600 (CST) 4, 16 -- Date: Fri, 10 Feb 2006 12:25:14 -0600 (CST) 4, 16 -- From: LDM Microscopy {ldmm-at-risc4.numis.northwestern.edu} 4, 16 -- To: MSA listserver {Microscopy-at-microscopy.com} 4, 16 -- Subject: Texts that combine TEM + scanning probe techniques 4, 16 -- In-Reply-To: {000001c494e3$2098d330$b99bd280-at-paklabpgrover} 4, 16 -- Message-ID: {Pine.GHP.4.63.0602101223340.24076-at-risc4.numis.northwestern.edu} 4, 16 -- References: {000001c494e3$2098d330$b99bd280-at-paklabpgrover} 4, 16 -- MIME-Version: 1.0 4, 16 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (anna8261-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, February 10, 2006 at 02:52:59 ---------------------------------------------------------------------------
Email: anna8261-at-yahoo.com Name: anna naghshegar
Organization: pooysh
Education: Graduate College
Location: tehran, iran
Question: what is stain solution for double heterostructure InP/InGaAsP?
Hello listservers, I realize that this might be a silly question but I was wondering if there was anyone here who knows what the structure of colloidal gold is.
Thanks, Carlo
-- Carlo Franco Bolivar Balane
Box 4058, 222 Church Street, or Wolfe Laboratory Wesleyan University Station Rm. 157, HA Laboratories Middletown, CT, 06459-4058 Wesleyan University
==============================Original Headers============================== 7, 32 -- From cbalane-at-wesleyan.edu Sun Feb 12 01:38:59 2006 7, 32 -- Received: from post1.wesleyan.edu (post1.wesleyan.edu [129.133.6.131]) 7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1C7cxSn031796 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 01:38:59 -0600 7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [129.133.6.192]) 7, 32 -- by post1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7dAPY015816 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:10 -0500 7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [127.0.0.1]) 7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7d045011036 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:00 -0500 7, 32 -- Received: (from apache-at-localhost) 7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11/Submit) id k1C7d0xP011034; 7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500 7, 32 -- Received: from 129.133.127.145 7, 32 -- (SquirrelMail authenticated user cbalane); 7, 32 -- by webmail.wesleyan.edu with HTTP; 7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500 (EST) 7, 32 -- Message-ID: {2201.129.133.127.145.1139729940.squirrel-at-129.133.127.145} 7, 32 -- Date: Sun, 12 Feb 2006 02:39:00 -0500 (EST) 7, 32 -- Subject: colloidal gold structure 7, 32 -- From: "Carlo Franco Balane-Bolivar" {cbalane-at-wesleyan.edu} 7, 32 -- To: Microscopy-at-microscopy.com 7, 32 -- User-Agent: SquirrelMail/1.4.3a-0.e3.1 7, 32 -- X-Mailer: SquirrelMail/1.4.3a-0.e3.1 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Type: text/plain;charset=iso-8859-1 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-Priority: 3 (Normal) 7, 32 -- Importance: Normal 7, 32 -- X-Wesleyan-MailScanner-Information: Please contact the ISP for more information 7, 32 -- X-Wesleyan-MailScanner: Found to be clean 7, 32 -- X-MailScanner-From: cbalane-at-wesleyan.edu ==============================End of - Headers==============================
In general multiply-twinned fcc domains I would have thought. Although depending on particle size, very small colloids can have structure forbidden by conventional bulk crystallography such as a 5-fold icosahedral or decahedral arrangements, plenty of literature around, both experimental and simulations. I seem to remember passivation of the gold 'cluster' can force the resulting colloid into certain structures. Generally Au over 3-4nm in diameter is mt-fcc though.
Neil Young Cluster Physics / Electron Microscopy Nanoscale Physics Research Laboratory School of Physics and Astronomy University of Birmingham UK
} Message Received: Feb 12 2006, 07:42 AM } From: cbalane-at-wesleyan.edu } To: neil-at-young8696.freeserve.co.uk } Cc: } Subject: [Microscopy] colloidal gold structure } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello listservers, } I realize that this might be a silly question but I was wondering if there } was anyone here who knows what the structure of colloidal gold is. } } Thanks, } Carlo } } } -- } Carlo Franco Bolivar Balane } } Box 4058, 222 Church Street, or Wolfe Laboratory } Wesleyan University Station Rm. 157, HA Laboratories } Middletown, CT, 06459-4058 Wesleyan University } } } } ==============================Original Headers============================== } 7, 32 -- From cbalane-at-wesleyan.edu Sun Feb 12 01:38:59 2006 } 7, 32 -- Received: from post1.wesleyan.edu (post1.wesleyan.edu [129.133.6.131]) } 7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1C7cxSn031796 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 01:38:59 -0600 } 7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [129.133.6.192]) } 7, 32 -- by post1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7dAPY015816 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:10 -0500 } 7, 32 -- Received: from pony1.wesleyan.edu (pony1.wesleyan.edu [127.0.0.1]) } 7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11) with ESMTP id k1C7d045011036 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 02:39:00 -0500 } 7, 32 -- Received: (from apache-at-localhost) } 7, 32 -- by pony1.wesleyan.edu (8.12.11/8.12.11/Submit) id k1C7d0xP011034; } 7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500 } 7, 32 -- Received: from 129.133.127.145 } 7, 32 -- (SquirrelMail authenticated user cbalane); } 7, 32 -- by webmail.wesleyan.edu with HTTP; } 7, 32 -- Sun, 12 Feb 2006 02:39:00 -0500 (EST) } 7, 32 -- Message-ID: {2201.129.133.127.145.1139729940.squirrel-at-129.133.127.145} } 7, 32 -- Date: Sun, 12 Feb 2006 02:39:00 -0500 (EST) } 7, 32 -- Subject: colloidal gold structure } 7, 32 -- From: "Carlo Franco Balane-Bolivar" {cbalane-at-wesleyan.edu} } 7, 32 -- To: Microscopy-at-microscopy.com } 7, 32 -- User-Agent: SquirrelMail/1.4.3a-0.e3.1 } 7, 32 -- X-Mailer: SquirrelMail/1.4.3a-0.e3.1 } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Type: text/plain;charset=iso-8859-1 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-Priority: 3 (Normal) } 7, 32 -- Importance: Normal } 7, 32 -- X-Wesleyan-MailScanner-Information: Please contact the ISP for more information } 7, 32 -- X-Wesleyan-MailScanner: Found to be clean } 7, 32 -- X-MailScanner-From: cbalane-at-wesleyan.edu } ==============================End of - Headers============================== } }
==============================Original Headers============================== 6, 25 -- From neil-at-young8696.freeserve.co.uk Sun Feb 12 06:20:41 2006 6, 25 -- Received: from smtp2.freeserve.com (smtp2.wanadoo.co.uk [193.252.22.157]) 6, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1CCKd7F018464 6, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 06:20:40 -0600 6, 25 -- Received: from me-wanadoo.net (localhost [127.0.0.1]) 6, 25 -- by mwinf3106.me.freeserve.com (SMTP Server) with ESMTP id 266D41C00085 6, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 13:20:37 +0100 (CET) 6, 25 -- Received: from wwinf3103 (wwinf3103 [172.22.158.30]) 6, 25 -- by mwinf3106.me.freeserve.com (SMTP Server) with ESMTP id 205A51C00083 6, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 13:20:37 +0100 (CET) 6, 25 -- X-ME-UUID: 20060212122037132.205A51C00083-at-mwinf3106.me.freeserve.com 6, 25 -- Message-ID: {30650493.1139746837118.JavaMail.www-at-wwinf3103} 6, 25 -- From: "Neil P. Young" {neil-at-young8696.freeserve.co.uk} 6, 25 -- Reply-To: neil-at-young8696.freeserve.co.uk 6, 25 -- To: Microscopy-at-microscopy.com 6, 25 -- Subject: RE: [Microscopy] colloidal gold structure 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=UTF-8 6, 25 -- Content-Transfer-Encoding: 7bit 6, 25 -- X-Originating-IP: [195.92.168.168] 6, 25 -- X-Wum-Nature: EMAIL-NATURE 6, 25 -- X-WUM-FROM: |~| 6, 25 -- X-WUM-TO: |~| 6, 25 -- X-WUM-REPLYTO: |~| 6, 25 -- Date: Sun, 12 Feb 2006 13:20:37 +0100 (CET) ==============================End of - Headers==============================
Karl, I hadn't seen any answer to your question so I'll give it a shot. For those of us who grew up with Be windows, K bigger than L seems normal. However, the norm for light element detectors is the opposite, at least if you normally operate at 20kV or so. I'm wondering if you've had a long-standing contamination problem with your light element window. Now that it's clean, you may be seeing the correct ratio and may find that your light element detection is also better.
The other thing to be certain of is that you are operating at the same kV as you were earlier. Lower kV will favor lower energy peaks and higher kV will favor higher energy peaks. It can be quite dramatic. Put a little graphite on a piece of Cu tape and collect a spectrum from an area containing both. Maintain a constant dead-time while collecting a spectrum at 30 kV and another at about 2 kV. You'd never know it's the same sample.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: hagglundk1-at-nku.edu [mailto:hagglundk1-at-nku.edu] Sent: Tuesday, January 24, 2006 10:33 AM To: kenconverse-at-qualityimages.biz
I have a colleague who recently experienced some down time on his SEM. The system went through repeated cycles of pump down, and venting and was eventually left powered down while waiting on a computer replacement.
After getting everything operating again, an oil film was found that had built up on the x-ray detector thin window. This was initially causing an unacceptable amount of background in the 0-3kV range of the x-ray signal as well as an overall low count rate. The collimator was removed and cleaned, which corrected the noise and count rate. They are in the process of replacing the roughing lines and cleaning the chamber to prevent further contamination. Now, when calibrating with a copper standard, the ratio of the L line signal versus the K lines is dramatically favoring the L. This was not the case before.
Any ideas why this might be happening and how to correct it?
Karl Hagglund Biological Sciences, SC-102B Northern Kentucky University, Nunn Drive Highland Heights, KY 41099 859-572-5238 http://semlab.nku.edu
==============================Original Headers============================== 5, 23 -- From hagglundk1-at-nku.edu Tue Jan 24 09:02:19 2006 5, 23 -- Received: from mailfe2.nku.edu (mailfe2.nku.edu [192.122.237.68]) 5, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k0OF2JlW001861 5, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2006 09:02:19 -0600 5, 23 -- Received: from mailfac1.nku.edu ([172.28.102.15]) by mailfe2.nku.edu with Microsoft SMTPSVC(6.0.3790.211); 5, 23 -- Tue, 24 Jan 2006 10:02:19 -0500 5, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 5, 23 -- Content-class: urn:content-classes:message 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="us-ascii" 5, 23 -- Subject: SEM x-ray peak ratios 5, 23 -- Date: Tue, 24 Jan 2006 10:02:18 -0500 5, 23 -- Message-ID: {F01E4499C4EC5842A8AB7198141AC72F3585FA-at-mailfac1.hh.nku.edu} 5, 23 -- X-MS-Has-Attach: 5, 23 -- X-MS-TNEF-Correlator: 5, 23 -- Thread-Topic: SEM x-ray peak ratios 5, 23 -- Thread-Index: AcYg9yuMm942rGb1QfKCevfjxyMD/g== 5, 23 -- From: "Karl Hagglund" {hagglundk1-at-nku.edu} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- X-OriginalArrivalTime: 24 Jan 2006 15:02:19.0222 (UTC) FILETIME=[2BE72F60:01C620F7] 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k0OF2JlW001861 ==============================End of - Headers==============================
___________________________________________________________ $0 Web Hosting with up to 200MB web space, 1000 MB Transfer 10 Personalized POP and Web E-mail Accounts, and much more. Signup at www.doteasy.com
==============================Original Headers============================== 22, 23 -- From kenconverse-at-qualityimages.biz Sun Feb 12 14:39:26 2006 22, 23 -- Received: from qualityimages.biz (dpmail16.doteasy.com [65.61.209.16]) 22, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1CKdPhn012116 22, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Sun, 12 Feb 2006 14:39:26 -0600 22, 23 -- Received: from Ken [68.64.242.101] by qualityimages.biz with ESMTP 22, 23 -- (SMTPD32-8.05) id AD6DA2CE00B8; Sun, 12 Feb 2006 12:41:17 -0800 22, 23 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 22, 23 -- To: {hagglundk1-at-nku.edu} , "MSA Listserver" {Microscopy-at-MSA.Microscopy.Com} 22, 23 -- Subject: RE: [Microscopy] SEM x-ray peak ratios 22, 23 -- Date: Sun, 12 Feb 2006 15:38:54 -0500 22, 23 -- Message-ID: {001701c63014$5fed1550$6501a8c0-at-Ken} 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; 22, 23 -- charset="us-ascii" 22, 23 -- X-Priority: 3 (Normal) 22, 23 -- X-MSMail-Priority: Normal 22, 23 -- X-Mailer: Microsoft Outlook, Build 10.0.6626 22, 23 -- Importance: Normal 22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 22, 23 -- In-Reply-To: {200601241533.k0OFX9f9007423-at-ns.microscopy.com} 22, 23 -- X-IMSTrailer: __IMail_7__ 22, 23 -- Content-Transfer-Encoding: 8bit 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1CKdPhn012116 ==============================End of - Headers==============================
A colleague has a Nikon SMZ-2T Microscope that he is trying to rescue. He is after a copy of the manual. If anyone can help pls contact him at hbeh-at-hotmail.com or via myself.
Thank you for your help
Regards George
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==============================Original Headers============================== 8, 27 -- From George.Theodossiou-at-amcor.com.au Sun Feb 12 18:58:09 2006 8, 27 -- Received: from aiti251.amcor.com.au (mail.amcor.com.au [202.14.180.248] (may be forged)) 8, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1D0w78V024534 8, 27 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Feb 2006 18:58:08 -0600 8, 27 -- Received: from aadcex0002.amcor.net (unverified) by aiti251.amcor.com.au 8, 27 -- (Content Technologies SMTPRS 4.3.19) with ESMTP id 8, 27 -- {T766fb3d9f0a0de98c8538-at-aiti251.amcor.com.au} for 8, 27 -- {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 12:03:18 +1100 8, 27 -- Received: from aadcex0004.amcor.net ([160.222.80.235]) by 8, 27 -- aadcex0002.amcor.net with Microsoft SMTPSVC (6.0.3790.211); Mon, 13 Feb 8, 27 -- 2006 11:58:05 +1100 8, 27 -- Received: from 160.222.214.35 ([160.222.214.35]) by aadcex0004.amcor.net 8, 27 -- ([160.222.80.235]) with Microsoft Exchange Server HTTP-DAV; Mon, 13 Feb 8, 27 -- 2006 00:58:05 +0000 8, 27 -- User-Agent: Microsoft-Entourage/11.2.1.051004 8, 27 -- Date: Mon, 13 Feb 2006 11:57:31 +1100 8, 27 -- Subject: Nikon Microscope 8, 27 -- From: "George.Theodossiou" {George.Theodossiou-at-amcor.com.au} 8, 27 -- To: {Microscopy-at-microscopy.com} 8, 27 -- Message-ID: {C01624AB.8E6%George.Theodossiou-at-Amcor.com.au} 8, 27 -- Thread-Topic: Nikon Microscope 8, 27 -- Thread-Index: AcYwOHehtgRappwrEdqDLgANkzYUMg== 8, 27 -- Mime-version: 1.0 8, 27 -- Content-type: text/plain; charset="US-ASCII" 8, 27 -- Content-transfer-encoding: 7bit 8, 27 -- X-OriginalArrivalTime: 13 Feb 2006 00:58:06.0019 (UTC) 8, 27 -- FILETIME=[8C80C930:01C63038] ==============================End of - Headers==============================
Thank you everybody who replied to my question regarding safety measures when working with uranyl acetate stained blocks.
To sum up the answers: The binding capacity of tissue to uranyl acetate is apparently low and therefore very little (if any) radioactivity can escape the blocks. However, when trimming I have been advised to wear gloves and a mask to prevent myself from inhaling chips from the specimen, and it seems necessary that we collect the chips and dispose of them as radioactive waste.
thank you
Gerd
Dr. Gerd Leitinger
Institut für Zellbiologie, Histologie und Embryologie Medizinische Universität Graz Harrachgasse 21 A-8010 Graz Austria
We are trying to viusalize infected erythrocytes (malarial parasite) on confocal microscope by indirect fluorescence without any fixation and washing is carried out with PBS
The immages are not satisfactory because fading/bleaching is fast though we are using % DABCO in 50% glycerol and secondly lot of background noise.
Most of the literature shows the fixation with ethanol/methenol but I would like to carry out the fixation with paraformaldehyde for the obvious reason and washing with MSM-PIPES with tris.
any suggestion???? or any body can describle the sample preparation for infected erythrocytes using paraformaldehy as a fixative and MSM PIPES as a washing buffer...
Does non fixation of specimen play any role in fast bleaching???
Regards Shrunali Scientist IMTECH, India
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==============================Original Headers============================== 10, 18 -- From aarti_harle-at-yahoo.co.in Mon Feb 13 04:53:08 2006 10, 18 -- Received: from web8308.mail.in.yahoo.com (web8308.mail.in.yahoo.com [202.43.219.220]) 10, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1DAr74m018713 10, 18 -- for {Microscopy-at-Microscopy.com} ; Mon, 13 Feb 2006 04:53:07 -0600 10, 18 -- Received: (qmail 90502 invoked by uid 60001); 13 Feb 2006 10:53:05 -0000 10, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 18 -- s=s1024; d=yahoo.co.in; 10, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 10, 18 -- b=XV1GTCKSYckbhHBodcI+9aRMHIyL3k7aSk/fx0siVLqY2GubzN4t3ETtsQ5SvUNHdvo0HZCbwY0LNwqIEVo5wG0A2w5hFGhRK2sdU4Sw5T+v7Wv8JD5Q0rRO14w39oy6TAwfWGnMr0IXewk2yCdWRCswRSZSwMNHWt722M1sryM= ; 10, 18 -- Message-ID: {20060213105305.90500.qmail-at-web8308.mail.in.yahoo.com} 10, 18 -- Received: from [203.197.210.215] by web8308.mail.in.yahoo.com via HTTP; Mon, 13 Feb 2006 02:53:05 PST 10, 18 -- Date: Mon, 13 Feb 2006 02:53:05 -0800 (PST) 10, 18 -- From: Aarti Harle {aarti_harle-at-yahoo.co.in} 10, 18 -- Subject: confocal microscopy 10, 18 -- To: Microscopy-at-Microscopy.com 10, 18 -- MIME-Version: 1.0 10, 18 -- Content-Type: text/plain; charset=iso-8859-1 10, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
You don't specify which fluorochrome you are using. If you are using FITC or Rhodamine, that is one of the reasons you are getting rapid fading. The Alexa488 and Alexa568 fluorochromes from Molecular Probes (Invitrogen) are far superior in this regard - especially for confocal. Molecular Probes also has a new anti-fade mounting medium called Prolong Gold but I don't have enough experience at the moment to discuss its efficacy. Fixation will not have any effect on bleaching but will contribute to background noise (especially glutaraldehyde). Generally 2% PF is okay in regards to background fluorescence and sometimes it is safe to add 0.1 - 02% glutaraldehyde. Aldehyde fixatives may, however, interfere with your antibody recognizing its epitope. good luck.
rote:
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Thomas E. Phillips, PhD Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400
I'm working on getting a Zeiss 940A up and running for my local high school. We are working on a shoe-string budget as one may expect from a small public high school.
I am looking for technical documentation for this instrument so that I can try and get it functioning. If anyone has schematics or any other technical documentation for instrument this I would greatly appreciate getting copies in some way.
If anyone has a similar instrument that would be able to donate it to us for a spare parts machine, we would come to pick it up. We are located in the Hudson Valley.
I am willing to pay for copies if need be, as well as transportation costs.
Thank you in advance,
dj
==============================Original Headers============================== 7, 22 -- From dljones-at-bestweb.net Mon Feb 13 09:22:44 2006 7, 22 -- Received: from smtp2.bestweb.net (smtp2.bestweb.net [209.94.103.44]) 7, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1DFMigH008985 7, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 09:22:44 -0600 7, 22 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1]) 7, 22 -- by smtp2.bestweb.net (Postfix) with ESMTP id 0659D1CD06 7, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 10:22:42 -0500 (EST) 7, 22 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96]) 7, 22 -- by smtp2.bestweb.net (Postfix) with ESMTP id 9164D1CCCB 7, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Feb 2006 10:22:41 -0500 (EST) 7, 22 -- Date: Mon, 13 Feb 2006 10:31:56 -0500 (Eastern Standard Time) 7, 22 -- From: "David L. Jones" {dljones-at-bestweb.net} 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- Subject: Zeiss 940A 7, 22 -- Message-ID: {Pine.WNT.4.62.0602131022110.1512-at-dlj} 7, 22 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 7, 22 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net 7, 22 -- X-Spam-Level: 7, 22 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed 7, 22 -- version=3.0.2 ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (roncastellano-at-adelphia.net) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, February 13, 2006 at 13:21:29 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both roncastellano-at-adelphia.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: roncastellano-at-adelphia.net Name: Ron Castellano
Organization: Wolfram Analytical Labs
Education: Undergraduate College
Location: 924 Fords Corner Road, Nanty Glo, PA 15943
Title: Assistance to setup a JOEL JSM-35C SEM
Question: I'm a retired assay technician(precious metal analysis) I have purchased an old SEM for private use in a small lab I am building here. I need someone to set up and test unit at my site. The unit is said to be operational when de-installed, it appears to be in good shape. Are there any technicians, perhaps a graduate student, who can assist me. Of course I am willing to pay any reasonable fee for this service. Ron Castellano
I am in need of a working video card for a Gatan 673 Wide TV Camera, vintage 1987. This camera system is used on a Philips CM12 for teaching purposes. The video card model is Schlumberger/Fairchild FAB-2-1-28 rev. F.
I will consider buying the controller if you do not wish to sell the card separately.
If you wish you can contact me offline.
Thanks in advance,
Fred Pearson
******************************************* Fred Pearson McMaster University Brockhouse Institute for Materials Research ABB-B145 1280 Main Street West Hamilton ON. Canada L8S 4M1
Lehigh University, Center for Advanced Materials and Nanotechnology, Bethlehem, PA is offering for sale a JEOL-6300F FEGSEM which will be decommissioned in mid-March 2006. The Microscope includes an Oxford/ISIS thin window EDS system, JEOL dry airlock and ARC64 digital imaging system, solid state backscatter detector, IR chamberview camera. Asking price: $60K Interested parties can contact Dr. Chris Kiely at chk5-at-lehigh.edu.
For technical questions regarding the microscope please contact Bill Mushock wim5-at-lehigh.edu
--
==============================Original Headers============================== 6, 22 -- From wim5-at-lehigh.edu Wed Feb 15 12:43:09 2006 6, 22 -- Received: from rain.CC.Lehigh.EDU (rain.CC.Lehigh.EDU [128.180.39.20]) 6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1FIh93u029868 6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 12:43:09 -0600 6, 22 -- Received: from lehigh.edu (Dyn055133.mat.Lehigh.EDU [128.180.55.133]) 6, 22 -- (authenticated bits=0) 6, 22 -- by rain.CC.Lehigh.EDU (8.13.5/8.13.5) with ESMTP id k1FIh9Uv018394 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Feb 2006 13:43:09 -0500 6, 22 -- Message-ID: {43F37517.5020702-at-lehigh.edu} 6, 22 -- Date: Wed, 15 Feb 2006 13:38:15 -0500 6, 22 -- From: William J Mushock {wim5-at-lehigh.edu} 6, 22 -- Organization: Lehigh University 6, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 6, 22 -- X-Accept-Language: en,en-US 6, 22 -- MIME-Version: 1.0 6, 22 -- To: Microscopy-at-microscopy.com 6, 22 -- Subject: JEOL JSM-6300f FEGSEM for sale 6, 22 -- Content-Type: text/plain; charset=us-ascii; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-Virus-Scanned: ClamAV version 0.88, clamav-milter version 0.87 on rain.CC.Lehigh.EDU 6, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I'm writing this as a response to Chris Kiely's post, but it is a very general point that probably bears repeating from time to time.
If a piece of equipment has been purchased by US Government funds (regardless of agency), then it is not permissible to use other US Government funds to re-purchase the same piece of equipment later. This is true even if title to the equipment has been passed to the holding instrumentation.
To use the specific example of the Lehigh SEM, if it was purchased through a government grant, I would not be able to buy it from them, regardless of how much use it would be to me, because the only funds I have available (at least for the purposes of my illustration!) are NSF funds. This is a limitation enforced on me, as the spender of Government funds, rather than on the seller (for they are - presumably - selling their own property), but it does mean that I need to know up front whether the instrument was purchased with any US Government funds (even if other funds were used as well) before I can decide whether I might be interested in it. I, and my institution, would be in major hot water should an auditor discover I had used government funds in this way (I suspect that here at MIT our internal systems would catch this before the sale went through, but that may not be true everywhere).
Tony Garratt-Reed.
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*********************************************** Anthony J. Garratt-Reed, M.A., D.Phil. MIT Room 13-1027 77 Massachusetts Avenue Cambridge, MA 02139-4307 USA
Because it's abstract time for both SCANNING 2006 and M&M, we know there is time pressure on all contributors. We are therefore extending the abstract deadline for SCANNING 2006 to March 1. Abstracts will appear in the Program Issue of SCANNING (March-April Issue) if received by March 1. Thank you.
For current program information and to download the meeting and hotel registration forms, please visit www.scanning.org.
This Question was submitted to Ask-A-Microscopist by (grmitch-at-netzero.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, February 15, 2006 at 20:08:01 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both grmitch-at-netzero.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: grmitch-at-netzero.com Name: Linda Mitchell
Organization: BPS Environmental Center
Education: K-8 Grade Grammar School
Location: Birmingham, Michigan USA
Title: Microorganisms!
Question: I will be teaching a unit on microscopic life to 5th graders next month, March (quite a cold month here in Michigan). My question for you: What is the best way to keep my microorganisms alive throughout the program? We obtain our samples directly from the 10 acres of the property here at the nature center. One of the samples is from our pond area and the second from the swamp woods area. We have no difficulty in finding a healthy sample, yet the problem that we often encounter is keeping them alive. We have supplies - lab pans, microscopes, even an aquarium that is our "indoor pond" when the water ices over. Do you have any feeding, climate tips that would help in these areas? This is a program that is enjoyed by both the staff and students - they are so amazed by what they find. Thanks for your input.
I'm out of the country right now, but I have been getting multiple questions about problems with the MM2006 server for submitting papers.
Please note that there was a HUGE spike in the paper submissions and the server is overloaded. As a consequence the MM2006 Executive committee has extended the deadline for paper submissions.
A note to this effect is appended below.
Nestor Your Friendly Neighborhood SysOp (in Australia for the moment).
The paper submission deadline for M&M 2006 in Chicago has been extended two days until 5:00pm Pacific Standard Time on Friday February 17, 2006. Due to an ongoing problem with the server today we have decided to extend the deadline to allow everyone the opportunity to submit their papers. Please try to access the paper submission site {http://bono.cup.org/} http://bono.cup.org/ later to register and submit your paper. Our sincerest apologies for any problems this may have caused.
Thanks, Paul Kotula Microscopy &Microanalysis 2006 Program Chair
Paul G. Kotula, Ph.D. Principal Member of Technical Staff Materials Characterization Department Sandia National Laboratories PO Box 5800, MS 0886 Albuquerque, NM 87185-0886
ph:(505) 844-8947
==============================Original Headers============================== 12, 11 -- From zaluzec-at-microscopy.com Thu Feb 16 14:52:48 2006 12, 11 -- Received: from [203.33.121.155] (msdvpn28.msd.anl.gov [130.202.238.92]) 12, 11 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GKqh4n008778 12, 11 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 14:52:45 -0600 12, 11 -- Mime-Version: 1.0 12, 11 -- Message-Id: {p06020404c01a95b6217e-at-[203.33.121.155]} 12, 11 -- Date: Fri, 17 Feb 2006 07:52:41 +1100 12, 11 -- To: microscopy-at-microscopy.com 12, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 12, 11 -- Subject: MM2006 Server OverLoad - Deadline extended 12, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sue.tyler-at-noaa.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sue.tyler-at-noaa.gov Name: Sue Tyler
Organization: NOAA
Title-Subject: [Filtered] Glass knife breaker
Question: I read the recent string on the glass knife makers and I was interested in the conclusion. We are interested in purchasing either the GKM or the Leica KMR. Any pros or cons would be appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both diller-at-stefan-diller.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: diller-at-stefan-diller.com Name: Stefan Diller
Organization: Scientific Photography
Title-Subject: [Filtered] Tracor Northern TN2000 EDS system
Question: Dear All, is anybody out there knowing some details or having some images available of an 1991 Tracor Northern TN2000 EDS system?
Is there still a possiblity to get service in Germany? Is there any possibility to get the EDS data out of the analyzer into a PC for saving, printing, emailing? What is the latest version of software for the TN2000?
Please reply offline, if convenient... I will do a summarizing for the list...
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both soleimanij-at-tbzmed.ac.ir as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: soleimanij-at-tbzmed.ac.ir Name: Jafar Soleimani Rad
Organization: University
Title-Subject: [Filtered] cutting a polymer
Question: We are having problem with cutting a polymer, Acrylonitril Butadien Styrene (ABS. After embedding in resin it dose not stick to it and becomes separated when trying to cut with ultramicrotom. It is also impossible to cut paraffin blocks. I would appreciate if you guide me with your experiences in this matter.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both b.farwell-at-unf.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: We are using a Molecular Imaging AFM and controller and are using "MI Metrology Series 2000 AFM System" program to interface to the AFM controller. The computer is having connection issues with the controller. Cables have been checked, and the setup disk has run on the controller, it gives 4 beeps, however the computer still can't connect. Ping gives no response. Anybody have any ideas? Thanks very much Brenton
I recently acquired a used Balzers MED 010 of unknown vintage. I am in need of the User Manual, or any such literature. I would like to get this unit back to operating condition.
Thanks,
John Crum NCMIR UCSD
==============================Original Headers============================== 4, 18 -- From jcrum-at-ncmir.ucsd.edu Thu Feb 16 15:52:19 2006 4, 18 -- Received: from ncmir.ucsd.edu (ncmir.ucsd.edu [132.239.16.23]) 4, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GLqIWF027723 4, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 15:52:19 -0600 4, 18 -- Received: from [192.168.10.237] (capisce [132.239.16.109]) 4, 18 -- by ncmir.ucsd.edu (8.11.7p1+Sun/8.11.7) with ESMTP id k1GLqIL04880 4, 18 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Feb 2006 13:52:18 -0800 (PST) 4, 18 -- Message-ID: {43F4F412.1060004-at-ncmir.ucsd.edu} 4, 18 -- Date: Thu, 16 Feb 2006 13:52:18 -0800 4, 18 -- From: John Crum {jcrum-at-ncmir.ucsd.edu} 4, 18 -- Organization: University of California San Diego CRBS/NCMIR 4, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Macintosh/20050317) 4, 18 -- X-Accept-Language: en-us, en 4, 18 -- MIME-Version: 1.0 4, 18 -- To: Microscopy-at-microscopy.com 4, 18 -- Subject: Wanted: Balzers MED 010 Manual 4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am looking for a used PSEM (or parts) to purchase. Preferably this system would be the original model manufactured by RJ Lee Instruments in the mid to late 90s. Anyone that can help me out, please email directly to andy-at-psemdoctor.com. Thank you,
Andrew L. Zobel
PSEMDOCTOR, LLC 117 Bryant Dr. Pittsburgh, PA 15235 Tel. 412-215-8906 Fax 412-241-3598 WWW.PSEMDOCTOR.COM
==============================Original Headers============================== 6, 22 -- From andy-at-psemdoctor.com Thu Feb 16 17:20:44 2006 6, 22 -- Received: from rwcrmhc14.comcast.net (rwcrmhc14.comcast.net [204.127.192.84]) 6, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1GNKinN025744 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 17:20:44 -0600 6, 22 -- Received: from azdesktop (c-24-3-48-75.hsd1.pa.comcast.net[24.3.48.75]) 6, 22 -- by comcast.net (rwcrmhc14) with SMTP 6, 22 -- id {20060216232043m1400qhsbqe} ; Thu, 16 Feb 2006 23:20:43 +0000 6, 22 -- Reply-To: {andy-at-psemdoctor.com} 6, 22 -- From: "Andrew L. Zobel" {andy-at-psemdoctor.com} 6, 22 -- To: {Microscopy-at-microscopy.com} 6, 22 -- Subject: [Microscopy] Looking for used PSEM 6, 22 -- Date: Thu, 16 Feb 2006 18:20:43 -0500 6, 22 -- Organization: PSEMDOCTOR, LLC 6, 22 -- Message-ID: {000701c6334f$9c923ba0$6501a8c0-at-azdesktop} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: text/plain; 6, 22 -- charset="iso-8859-1" 6, 22 -- X-Mailer: Microsoft Office Outlook 11 6, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 6, 22 -- Thread-Index: AcYzTv46OmN0PyDUSIGoUkBwqvLP/AAAFumw 6, 22 -- Content-Transfer-Encoding: 8bit 6, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1GNKinN025744 ==============================End of - Headers==============================
Hi Sue We have the Leica glass knife breaker since 2001. We are very happy with it. Reliable, good knives! Our users like it in preference to our old workhorse LKB knife breakers. Elaine
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-- Dr. Elaine Humphrey Director, BioImaging Facility President, Microscopy Society of Canada (2003-2005) University of British Columbia 6270 University Blvd, mail-stop Botany Vancouver, BC CANADA, V6T 1Z4 Phone: 604-822-3354 FAX: 604-822-6089 e-mail: ech-at-interchange.ubc.ca website: www.emlab.ubc.ca
==============================Original Headers============================== 4, 29 -- From ech-at-interchange.ubc.ca Thu Feb 16 19:56:45 2006 4, 29 -- Received: from mta1.mail-relay.ubc.ca (mta1.mail-relay.ubc.ca [137.82.45.2]) 4, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1H1uiLA004198 4, 29 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 19:56:44 -0600 4, 29 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 4, 29 -- by mta1.mail-relay.ubc.ca (8.12.11/8.12.11) with ESMTP id k1H1ufaE023680 4, 29 -- for {microscopy-at-microscopy.com} ; Thu, 16 Feb 2006 17:56:41 -0800 (PST) 4, 29 -- (envelope-from ech-at-interchange.ubc.ca) 4, 29 -- Received: from [137.82.85.193] (echpowerbook.emlab.ubc.ca [137.82.85.193]) 4, 29 -- by smtp.interchange.ubc.ca 4, 29 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 4, 29 -- with ESMTPA id {0IUT00CU86QGQU-at-smtp.interchange.ubc.ca} for 4, 29 -- microscopy-at-microscopy.com; Thu, 16 Feb 2006 17:56:41 -0800 (PST) 4, 29 -- Date: Thu, 16 Feb 2006 17:56:38 -0800 4, 29 -- From: Elaine Humphrey {ech-at-interchange.ubc.ca} 4, 29 -- Subject: Re: [Microscopy] viaWWW: Glass Knife Breaker 4, 29 -- In-reply-to: {200602162058.k1GKw9iM025479-at-ns.microscopy.com} 4, 29 -- X-Sender: ech-at-mail.interchange.ubc.ca 4, 29 -- To: microscopy-at-microscopy.com 4, 29 -- Cc: sue.tyler-at-noaa.gov 4, 29 -- Message-id: {a06100504c01adcc79da1-at-[137.82.85.193]} 4, 29 -- MIME-version: 1.0 4, 29 -- Content-type: text/plain; format=flowed; charset=us-ascii 4, 29 -- References: {200602162058.k1GKw9iM025479-at-ns.microscopy.com} 4, 29 -- X-UBC-Scanned: Sophos PureMessage 4.7.1.128075, Antispam-Engine: 2.1.0.0, Antispam-Data: 2006.02.16.164605 4, 29 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 4, 29 -- X-PerlMx-Spam: Probability=7%, Report=IP_HTTP_ADDR 0, __C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_CRUFT 0 4, 29 -- X-Spam-Level: 4, 29 -- X-Spam-Flag: No ==============================End of - Headers==============================
A colleague is looking for the instruction manual for a Bausch & Lomb Research I metallograph. If anybody can help him in this quest please respond directly to him, Gregory Dexter at greg-at-met-sol.com .
Thanks,
Jeff Stewart Metallographic Laboratory Manager Stern-Leach Company 49 Pearl Street Attleboro, MA 02703 508-222-7400 x1329
==============================Original Headers============================== 5, 21 -- From jeff-at-metallography.com Fri Feb 17 08:43:23 2006 5, 21 -- Received: from Q1-ABI-TX.sanimail.com (mail6.mailsystem.us [67.97.234.238]) 5, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HEhM02018323 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 08:43:23 -0600 5, 21 -- Received: (qmail 68083 invoked by uid 1010); 17 Feb 2006 15:00:57 -0000 5, 21 -- Received: from unknown (HELO jstewart) (4.154.213.130) 5, 21 -- by mail6.mailsystem.us with SMTP; 17 Feb 2006 15:00:57 -0000 5, 21 -- Message-ID: {001801c633d0$9895b500$959610ac-at-sternleach.com} 5, 21 -- Reply-To: "Jeff Stewart" {jeff-at-metallography.com} 5, 21 -- From: "Jeff Stewart" {jeff-at-metallography.com} 5, 21 -- To: {Microscopy-at-microscopy.com} 5, 21 -- Subject: Seeking manual for B&L Research I metallograph 5, 21 -- Date: Fri, 17 Feb 2006 09:41:06 -0500 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: text/plain; 5, 21 -- charset="iso-8859-1" 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-Priority: 3 5, 21 -- X-MSMail-Priority: Normal 5, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1409 5, 21 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2800.1409 ==============================End of - Headers==============================
For years we have been wondering where the dark material was coming from that accumulated in our water filters. These are filters in the closed circuit lines between our microscopes and water recirculating units. The lines are mainly in the ceiling or totally insulated as they run down the walls in the scope rooms. Imaging our surprise when we went to do a minor repair on one and watched as the plumber removed a regulator and inserted a piece of galvanized pipe.
Apparently when the building was built (20 years ago), the contractor used galvanized pipe when copper had been specified. As it was hidden, we did not know about the switch. All visible lines hooking up the chiller compressor cooling with building water were copper.
Well now these galvanized lines are really breaking down and clogging the water pumps. We intend to replace all but were questioning whether it would be best to replace with copper or PVC piping. Any suggestions?
One concern was whether there would be the need to acid clean these lines in the future (this is done routinely to the compressor lines to remove mineral build-up). Since it is closed circuit, we should not accumulate large amounts of minerals even though tap or deionized water will be used for the system. We also can control algae growth with chemicals. Any suggestions on this and should this dictate which material is used for the pipes?
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 8, 21 -- From dsherman-at-purdue.edu Fri Feb 17 11:51:22 2006 8, 21 -- Received: from exchange.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223]) 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HHpMgE030390 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 11:51:22 -0600 8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 8, 21 -- Fri, 17 Feb 2006 12:51:21 -0500 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ; 8, 21 -- Fri, 17 Feb 2006 17:51:21 +0000 8, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004 8, 21 -- Date: Fri, 17 Feb 2006 12:51:20 -0500 8, 21 -- Subject: Microscope cooling lines 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 21 -- Message-ID: {C01B7748.D5AB%dsherman-at-purdue.edu} 8, 21 -- Thread-Topic: Microscope cooling lines 8, 21 -- Thread-Index: AcYz6sIxAPacp5/eEdqwBAARJN08Mg== 8, 21 -- Mime-version: 1.0 8, 21 -- Content-type: text/plain; 8, 21 -- charset="US-ASCII" 8, 21 -- Content-transfer-encoding: 7bit 8, 21 -- X-OriginalArrivalTime: 17 Feb 2006 17:51:21.0666 (UTC) FILETIME=[C32F5220:01C633EA] ==============================End of - Headers==============================
I wonder if anyone has a manual for the Denton DV-502A vacuum evaporator that I can borrow. Ours was lost during moving to storage and now I have to set it up again. It would be nice to make sure I set it up correctly. Denton can supply the manual for $250 so I am looking at other less expensive ways first. Thanks a lot!
-- Pang (Wai Pang Chan, wpchan-at-u.washington.edu, PAB A087, 206-685-1519) The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
==============================Original Headers============================== 3, 19 -- From wpchan-at-u.washington.edu Fri Feb 17 12:15:44 2006 3, 19 -- Received: from mxout5.cac.washington.edu (mxout5.cac.washington.edu [140.142.32.135]) 3, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HIFhWL007641 3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 12:15:44 -0600 3, 19 -- Received: from homer24.u.washington.edu (homer24.u.washington.edu [140.142.15.10]) 3, 19 -- by mxout5.cac.washington.edu (8.13.5+UW05.10/8.13.5+UW05.09) with ESMTP id k1HIFg5m011897 3, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 10:15:43 -0800 3, 19 -- Received: from localhost (wpchan-at-localhost) 3, 19 -- by homer24.u.washington.edu (8.13.5+UW05.10/8.13.5+Submit) with ESMTP id k1HIFgup027346 3, 19 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 10:15:42 -0800 3, 19 -- Date: Fri, 17 Feb 2006 10:15:42 -0800 (PST) 3, 19 -- From: "W. Chan" {wpchan-at-u.washington.edu} 3, 19 -- To: microscopy-at-microscopy.com 3, 19 -- Subject: manual for Denton DV-502A 3, 19 -- Message-ID: {Pine.LNX.4.64.0602171008220.7757-at-homer24.u.washington.edu} 3, 19 -- MIME-Version: 1.0 3, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 3, 19 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, Report='__C230066_P5 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_7 0' ==============================End of - Headers==============================
For our new IMAGE building, I specified PVC piping for the closed circ loop between the water chillers and scopes. We still notice a greenish sludge building up in the water filters (takes about 6-8 months to become significant) but I am certain that this is coming from the EM (copper cooling coils and iron connections---} electrolytic reaction). The EM service people told us that if we ever used acid to clean the lines that they would no longer warranty the microscope. The PVC lines are perfectly clean.
JB
} For years we have been wondering where the dark material was coming from } that accumulated in our water filters. These are filters in the closed } circuit lines between our microscopes and water recirculating units. The } lines are mainly in the ceiling or totally insulated as they run down the } walls in the scope rooms. Imaging our surprise when we went to do a minor } repair on one and watched as the plumber removed a regulator and inserted a } piece of galvanized pipe. } } Apparently when the building was built (20 years ago), the contractor } used galvanized pipe when copper had been specified. As it was hidden, we } did not know about the switch. All visible lines hooking up the chiller } compressor cooling with building water were copper. } } Well now these galvanized lines are really breaking down and clogging the } water pumps. We intend to replace all but were questioning whether it would } be best to replace with copper or PVC piping. Any suggestions? } } One concern was whether there would be the need to acid clean these lines in } the future (this is done routinely to the compressor lines to remove mineral } build-up). Since it is closed circuit, we should not accumulate large } amounts of minerals even though tap or deionized water will be used for the } system. We also can control algae growth with chemicals. Any suggestions on } this and should this dictate which material is used for the pipes? } } Debby } } Debby Sherman, Manager Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www3.agriculture.purdue.edu/microscopy } } } ==============================Original Headers============================== } 8, 21 -- From dsherman-at-purdue.edu Fri Feb 17 11:51:22 2006 } 8, 21 -- Received: from exchange.purdue.edu } (1061exfe02.adpc.purdue.edu [128.210.63.223]) } 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k1HHpMgE030390 } 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 } 11:51:22 -0600 } 8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by } exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); } 8, 21 -- Fri, 17 Feb 2006 12:51:21 -0500 } 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by } EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server } exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange } Server HTTP-DAV ; } 8, 21 -- Fri, 17 Feb 2006 17:51:21 +0000 } 8, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004 } 8, 21 -- Date: Fri, 17 Feb 2006 12:51:20 -0500 } 8, 21 -- Subject: Microscope cooling lines } 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} } 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 8, 21 -- Message-ID: {C01B7748.D5AB%dsherman-at-purdue.edu} } 8, 21 -- Thread-Topic: Microscope cooling lines } 8, 21 -- Thread-Index: AcYz6sIxAPacp5/eEdqwBAARJN08Mg== } 8, 21 -- Mime-version: 1.0 } 8, 21 -- Content-type: text/plain; } 8, 21 -- charset="US-ASCII" } 8, 21 -- Content-transfer-encoding: 7bit } 8, 21 -- X-OriginalArrivalTime: 17 Feb 2006 17:51:21.0666 (UTC) } FILETIME=[C32F5220:01C633EA] } ==============================End of - Headers==============================
-- ############################################################## John J. Bozzola, Ph.D., Director I.M.A.G.E. (Integrated Microscopy & Graphics Expertise) 750 Communications Drive - MC 4402 Southern Illinois University Carbondale, IL 62901 U.S.A. Phone: 618-453-3730 Email: bozzola-at-siu.edu Web: http://www.siu.edu/~image/ ##############################################################
==============================Original Headers============================== 9, 18 -- From bozzola-at-siu.edu Fri Feb 17 12:57:04 2006 9, 18 -- Received: from abbmta2.siu.edu (abbmta2.siu.edu [131.230.254.206]) 9, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HIv3LI017656 9, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 12:57:04 -0600 9, 18 -- Received: from [131.230.177.142] (ws177142.microscope.siu.edu [131.230.177.142]) 9, 18 -- by abbmta2.siu.edu (Switch-3.1.6/Switch-3.1.6) with ESMTP id k1HIv252005395 9, 18 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 12:57:03 -0600 (CST) 9, 18 -- Mime-Version: 1.0 9, 18 -- X-Sender: bozzola-at-saluki-mail.siu.edu 9, 18 -- Message-Id: {p0611040bc01bcb431cdd-at-[131.230.177.142]} 9, 18 -- In-Reply-To: {200602171754.k1HHsAVw032278-at-ns.microscopy.com} 9, 18 -- References: {200602171754.k1HHsAVw032278-at-ns.microscopy.com} 9, 18 -- Date: Fri, 17 Feb 2006 12:57:00 -0600 9, 18 -- To: Microscopy-at-msa.microscopy.com 9, 18 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 9, 18 -- Subject: Re: [Microscopy] Microscope cooling lines 9, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 9, 18 -- X-MASF: 0.00% ==============================End of - Headers==============================
Another material you may wish to consider is called PEX, which is a cross-linked polyethylene. I don't know too much about its characteristics, except that it's very smooth inside, which should retard crud accumulation and it's more opaque than white pvc. It may be worth checking out.
Paul
-------------------------------------------------------------------------- Famous Last Words Department: "I did not get my Spaghetti-O's, I got spaghetti. I want the press to know this." ~~ Thomas J. Grasso, d. March 20, 1995 Executed by injection, Oklahoma.
==============================Original Headers============================== 7, 21 -- From pgrover-at-bilbo.bio.purdue.edu Fri Feb 17 13:19:13 2006 7, 21 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 7, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJJCk8027344 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:19:12 -0600 7, 21 -- Received: from paklabpgrover (dhcp155-122.bio.purdue.edu [128.210.155.122]) 7, 21 -- by mailhub128.itcs.purdue.edu (8.13.4/8.13.4/internal-smtp) with ESMTP id k1HJJCLp010612 7, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 14:19:12 -0500 7, 21 -- From: "pgrover" {pgrover-at-bilbo.bio.purdue.edu} 7, 21 -- To: {microscopy-at-microscopy.com} 7, 21 -- Subject: re: microscope cooling lines 7, 21 -- Date: Fri, 17 Feb 2006 14:19:17 -0500 7, 21 -- Message-ID: {000301c633f7$0bb24910$7a9bd280-at-paklabpgrover} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset="us-ascii" 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Mailer: Microsoft Office Outlook 11 7, 21 -- Thread-Index: AcYz9wt9iKTkG2SVRtGWvOlC3m8itw== 7, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.2180 7, 21 -- X-PMX-Version: 4.7.1.128075 7, 21 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
It would be good to know what is the operating temperature, pressure, flow rate and the characteristics of the water feed for make-up.
There is no material in-compatibility between copper piping and PVC piping. They can be used in the same water curcuit.
If you want to know if there is a problem using copper, you can take some of the water out of the system and see how much copper is present. You need to have a base line of copper from the water source, so you should take a water sample from the source also. There is likely not a problem, it depends upon your water chemistry. Knowing your water chemistry is fundamental to knowing what piping is preferred.
You may have building code restrictions regarding PVC piping that is hidden, which may be the reason the orginal contractor put in galvanized piping. You will have to look at what codes apply to where you are.
Regarding acid cleaning, you should know what your deposits are in your piping before deciding how to clean them. As an FYI, copper will generally corrode at pH's below about 6.3 or so. There are low pH cleaners that can be used with copper, but they contain corrosion inhibitors.
dj
On Fri, 17 Feb 2006 dsherman-at-purdue.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Listers, } } For years we have been wondering where the dark material was coming from } that accumulated in our water filters. These are filters in the closed } circuit lines between our microscopes and water recirculating units. The } lines are mainly in the ceiling or totally insulated as they run down the } walls in the scope rooms. Imaging our surprise when we went to do a minor } repair on one and watched as the plumber removed a regulator and inserted a } piece of galvanized pipe. } } Apparently when the building was built (20 years ago), the contractor } used galvanized pipe when copper had been specified. As it was hidden, we } did not know about the switch. All visible lines hooking up the chiller } compressor cooling with building water were copper. } } Well now these galvanized lines are really breaking down and clogging the } water pumps. We intend to replace all but were questioning whether it would } be best to replace with copper or PVC piping. Any suggestions? } } One concern was whether there would be the need to acid clean these lines in } the future (this is done routinely to the compressor lines to remove mineral } build-up). Since it is closed circuit, we should not accumulate large } amounts of minerals even though tap or deionized water will be used for the } system. We also can control algae growth with chemicals. Any suggestions on } this and should this dictate which material is used for the pipes? } } Debby } } Debby Sherman, Manager Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www3.agriculture.purdue.edu/microscopy } } } ==============================Original Headers============================== } 8, 21 -- From dsherman-at-purdue.edu Fri Feb 17 11:51:22 2006 } 8, 21 -- Received: from exchange.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223]) } 8, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HHpMgE030390 } 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 11:51:22 -0600 } 8, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); } 8, 21 -- Fri, 17 Feb 2006 12:51:21 -0500 } 8, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ; } 8, 21 -- Fri, 17 Feb 2006 17:51:21 +0000 } 8, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004 } 8, 21 -- Date: Fri, 17 Feb 2006 12:51:20 -0500 } 8, 21 -- Subject: Microscope cooling lines } 8, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} } 8, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 8, 21 -- Message-ID: {C01B7748.D5AB%dsherman-at-purdue.edu} } 8, 21 -- Thread-Topic: Microscope cooling lines } 8, 21 -- Thread-Index: AcYz6sIxAPacp5/eEdqwBAARJN08Mg== } 8, 21 -- Mime-version: 1.0 } 8, 21 -- Content-type: text/plain; } 8, 21 -- charset="US-ASCII" } 8, 21 -- Content-transfer-encoding: 7bit } 8, 21 -- X-OriginalArrivalTime: 17 Feb 2006 17:51:21.0666 (UTC) FILETIME=[C32F5220:01C633EA] } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 25 -- From dljones-at-bestweb.net Fri Feb 17 13:24:26 2006 10, 25 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net [209.94.103.46]) 10, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJOOlm003358 10, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:24:26 -0600 10, 25 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1]) 10, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id 747541CD06; 10, 25 -- Fri, 17 Feb 2006 14:24:23 -0500 (EST) 10, 25 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96]) 10, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id CAD7C1CCC2; 10, 25 -- Fri, 17 Feb 2006 14:24:21 -0500 (EST) 10, 25 -- Date: Fri, 17 Feb 2006 14:33:39 -0500 (Eastern Standard Time) 10, 25 -- From: "David L. Jones" {dljones-at-bestweb.net} 10, 25 -- To: dsherman-at-purdue.edu 10, 25 -- cc: Microscopy-at-microscopy.com 10, 25 -- Subject: Re: [Microscopy] Microscope cooling lines 10, 25 -- In-Reply-To: {200602171802.k1HI2DeL005131-at-ns.microscopy.com} 10, 25 -- Message-ID: {Pine.WNT.4.62.0602171405030.1080-at-dlj} 10, 25 -- References: {200602171802.k1HI2DeL005131-at-ns.microscopy.com} 10, 25 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 10, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net 10, 25 -- X-Spam-Level: 10, 25 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed 10, 25 -- version=3.0.2 ==============================End of - Headers==============================
On Feb 17, 2006, at 9:51 AM, dsherman-at-purdue.edu wrote:
} For years we have been wondering where the dark material was coming } from } that accumulated in our water filters. These are filters in the closed } circuit lines between our microscopes and water recirculating units. } The } lines are mainly in the ceiling or totally insulated as they run down } the } walls in the scope rooms. Imaging our surprise when we went to do a } minor } repair on one and watched as the plumber removed a regulator and } inserted a } piece of galvanized pipe. } } Apparently when the building was built (20 years ago), the } contractor } used galvanized pipe when copper had been specified. As it was } hidden, we } did not know about the switch. All visible lines hooking up the } chiller } compressor cooling with building water were copper. } } Well now these galvanized lines are really breaking down and clogging } the } water pumps. We intend to replace all but were questioning whether it } would } be best to replace with copper or PVC piping. Any suggestions? } } One concern was whether there would be the need to acid clean these } lines in } the future (this is done routinely to the compressor lines to remove } mineral } build-up). Since it is closed circuit, we should not accumulate large } amounts of minerals even though tap or deionized water will be used } for the } system. We also can control algae growth with chemicals. Any } suggestions on } this and should this dictate which material is used for the pipes? } Dear Debby, If you intend to clean the lines with acid, I suggest PVC, since Cu can be etched at low pH. In addition to floating some dichlorophene for algal control, we add a corrosion inhibitor. We have been using a Mo-based formula, which was available from Aqua Labs on the East coast and from Skasol on the West coast, so find a distributor in your area. I think that either Aqua or Skasol would be able to give you that info. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Feb 17 13:36:46 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJakQG013933 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 13:36:46 -0600 4, 22 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 040B535C5A 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:36:46 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id AC201340D7 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:36:43 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200602171751.k1HHpVq9030559-at-ns.microscopy.com} 4, 22 -- References: {200602171751.k1HHpVq9030559-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {6f18d2977f3ac75cf6e6159ba16304e9-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] Microscope cooling lines 4, 22 -- Date: Fri, 17 Feb 2006 11:44:38 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
On Feb 17, 2006, at 11:19 AM, pgrover-at-bilbo.bio.purdue.edu wrote:
} Another material you may wish to consider is called PEX, which is a } cross-linked polyethylene. I don't know too much about its } characteristics, } except that it's very smooth inside, which should retard crud } accumulation } and it's more opaque than white pvc. It may be worth checking out. } Dear Paul, PEX sounds like a better material than PVC. It should be essentially inert--I expect that it will withstand concentrated bases and acids and would be unaffected by organic solvents (not that one would want to run those through the scope lines). Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Fri Feb 17 13:42:57 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJgvr6022901 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 13:42:57 -0600 4, 22 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 28C8235764 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:42:57 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 4558E340D7 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Fri, 17 Feb 2006 11:42:56 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200602171919.k1HJJIaZ027514-at-ns.microscopy.com} 4, 22 -- References: {200602171919.k1HJJIaZ027514-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {929b43e7c910fd8036e0032d59e72528-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] re: microscope cooling lines 4, 22 -- Date: Fri, 17 Feb 2006 11:50:51 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
As an FYI, if you have copper based and iron based materials mixed in the same system, the iron will corrode preferentially through galvanic coupling, not the copper. In fact, the iron becomes a sacrifical anode protecting the copper from corrosion. Any time you have those two materials in the same system, you should have dielectric couplings between the two or you will actively corrode the ferrous based material.
If you are having a copper corrosion problem, you should be looking elsewhere for the cause...
dj
On Fri, 17 Feb 2006 bozzola-at-siu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Debby, } } For our new IMAGE building, I specified PVC piping for the closed } circ loop between the water chillers and scopes. We still notice a } greenish sludge building up in the water filters (takes about 6-8 } months to become significant) but I am certain that this is coming } from the EM (copper cooling coils and iron } connections---} electrolytic reaction). The EM service people told us } that if we ever used acid to clean the lines that they would no } longer warranty the microscope. The PVC lines are perfectly clean. } } JB }
==============================Original Headers============================== 7, 25 -- From dljones-at-bestweb.net Fri Feb 17 13:43:51 2006 7, 25 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net [209.94.103.46]) 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HJhoE0024591 7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:43:51 -0600 7, 25 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1]) 7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id EDFB81CD04; 7, 25 -- Fri, 17 Feb 2006 14:43:49 -0500 (EST) 7, 25 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96]) 7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id 7E4831CCC2; 7, 25 -- Fri, 17 Feb 2006 14:43:48 -0500 (EST) 7, 25 -- Date: Fri, 17 Feb 2006 14:53:06 -0500 (Eastern Standard Time) 7, 25 -- From: "David L. Jones" {dljones-at-bestweb.net} 7, 25 -- To: bozzola-at-siu.edu 7, 25 -- cc: Microscopy-at-microscopy.com 7, 25 -- Subject: Re: [Microscopy] Re: Microscope cooling lines 7, 25 -- In-Reply-To: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com} 7, 25 -- Message-ID: {Pine.WNT.4.62.0602171446420.1080-at-dlj} 7, 25 -- References: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com} 7, 25 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 7, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net 7, 25 -- X-Spam-Level: 7, 25 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed 7, 25 -- version=3.0.2 ==============================End of - Headers==============================
Along these lines, I noticed a lot more copper leaching into the lines when I used deionized water than regular or filtered tap water.
Secondly, Why hasn't anyone suggested using antifreeze? Antifreeze intended for cars should have corrosion inhibitors and should be suitable for this purpose.
I have still gotten green particles regardless of whether antifreeze was used or not and have regularly made it a practice to clean the filters in the lines once a semester. Jerry Calvin
At 1:46 PM -0600 2/17/06, dljones-at-bestweb.net wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- **************************** Jerry G. Calvin Science Support Technician Box 0731 Biology Department Vassar College 124 Raymond Avenue Poughkeepsie, NY 12604-0731
} PEX sounds like a better material than PVC. It should be essentially
Beware that PEX will degrade over time in sunlight (ultraviolet). That said, I have PEX in my house instead of Cu, works well and is easy to run, however all transitions through the walls are Cu fittings so the PEX stays in the dark.
Scott -- ----------------------------------------------------------------------- Scott D. Davilla Phone: 919 489-1757 ext 13 (tel) 4pi Analysis, Inc. Fax: 919 489-1487 (fax) 3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com Durham, North Carolina 27707-2534 web: http://www.4pi.com
==============================Original Headers============================== 4, 16 -- From davilla-at-4pi.com Fri Feb 17 15:17:27 2006 4, 16 -- Received: from mx.4pi.com (mx.4pi.com [24.172.19.59]) 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1HLHQjh021101 4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 15:17:27 -0600 4, 16 -- Received: from [24.172.19.62] (HELO [192.168.9.10]) 4, 16 -- by mx.4pi.com (Stalker SMTP Server 1.8b9d14) 4, 16 -- with ESMTP id S.0000850373 for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:17:26 -0500 4, 16 -- Mime-Version: 1.0 4, 16 -- Message-Id: {p06230957c01bece6d048-at-[192.168.9.10]} 4, 16 -- In-Reply-To: {200602171943.k1HJh1eC023022-at-ns.microscopy.com} 4, 16 -- References: {200602171943.k1HJh1eC023022-at-ns.microscopy.com} 4, 16 -- Date: Fri, 17 Feb 2006 16:17:38 -0500 4, 16 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 4, 16 -- From: "Scott D. Davilla" {davilla-at-4pi.com} 4, 16 -- Subject: Re: [Microscopy] microscope cooling lines 4, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Yes, it is not unusual to find more copper leaching into the lines with deionized water than tap water. It does depend upon the tap water chemistry.
I would not recommend antifreeze for cars, however, I would recommend to use HVAC grade propylene glycol. That would include inhibitors to protect the metals in the system and it includes various stablizers for the glycol. I guess if there are glycols for cars that are propylene glycol based with inhibitors, those may be OK. I most certainly would not use ethylene glycol based antifreeze.
I would ask you what kind of antifreeze you are using and have you done an EDS spectrum of your green deposit from you filters? I would think further discussion of your specifics may best be addressed off the list...
dj
On Fri, 17 Feb 2006, Jerry Calvin wrote:
} Along these lines, I noticed a lot more copper leaching into the lines when } I used deionized water than regular or filtered tap water. } } Secondly, Why hasn't anyone suggested using antifreeze? Antifreeze intended } for cars should have corrosion inhibitors and should be suitable for this } purpose. } } I have still gotten green particles regardless of whether antifreeze was used } or not and have regularly made it a practice to clean the filters in the } lines once a semester. Jerry Calvin } } } } At 1:46 PM -0600 2/17/06, dljones-at-bestweb.net wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } JB, } } } } As an FYI, if you have copper based and iron based materials mixed in the } } same } } system, the iron will corrode preferentially through galvanic coupling, not } } the } } copper. In fact, the iron becomes a sacrifical anode protecting the copper } } from } } corrosion. Any time you have those two materials in the same system, you } } should } } have dielectric couplings between the two or you will actively corrode the } } ferrous based material. } } } } If you are having a copper corrosion problem, you should be looking } } elsewhere } } for the cause... } } } } dj } } } } On Fri, 17 Feb 2006 bozzola-at-siu.edu wrote: } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Debby, } } } } } } For our new IMAGE building, I specified PVC piping for the closed } } } circ loop between the water chillers and scopes. We still notice a } } } greenish sludge building up in the water filters (takes about 6-8 } } } months to become significant) but I am certain that this is coming } } } from the EM (copper cooling coils and iron } } } connections---} electrolytic reaction). The EM service people told us } } } that if we ever used acid to clean the lines that they would no } } } longer warranty the microscope. The PVC lines are perfectly clean. } } } } } } JB } } } } } } } } } ==============================Original } } Headers============================== } } 7, 25 -- From dljones-at-bestweb.net Fri Feb 17 13:43:51 2006 } } 7, 25 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net } } [209.94.103.46]) } } 7, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } k1HJhoE0024591 } } 7, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 13:43:51 } } -0600 } } 7, 25 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1]) } } 7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id EDFB81CD04; } } 7, 25 -- Fri, 17 Feb 2006 14:43:49 -0500 (EST) } } 7, 25 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net } } [71.249.9.96]) } } 7, 25 -- by smtp2.bestweb.net (Postfix) with ESMTP id 7E4831CCC2; } } 7, 25 -- Fri, 17 Feb 2006 14:43:48 -0500 (EST) } } 7, 25 -- Date: Fri, 17 Feb 2006 14:53:06 -0500 (Eastern Standard Time) } } 7, 25 -- From: "David L. Jones" {dljones-at-bestweb.net} } } 7, 25 -- To: bozzola-at-siu.edu } } 7, 25 -- cc: Microscopy-at-microscopy.com } } 7, 25 -- Subject: Re: [Microscopy] Re: Microscope cooling lines } } 7, 25 -- In-Reply-To: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com} } } 7, 25 -- Message-ID: {Pine.WNT.4.62.0602171446420.1080-at-dlj} } } 7, 25 -- References: {200602171902.k1HJ2UNa024787-at-ns.microscopy.com} } } 7, 25 -- X-X-Sender: dljones-at-pop-croton.bestweb.net } } 7, 25 -- MIME-Version: 1.0 } } 7, 25 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } } 7, 25 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on } } smtp2-1.bestweb.net } } 7, 25 -- X-Spam-Level: } } 7, 25 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED } } autolearn=failed } } 7, 25 -- version=3.0.2 } } ==============================End of - } } Headers============================== } } } -- } **************************** } Jerry G. Calvin } Science Support Technician } Box 0731 Biology Department } Vassar College } 124 Raymond Avenue } Poughkeepsie, NY 12604-0731 } } (845) 437-7423 - Office } (845) 437-7424 - Confocal Room } FAX: (845) 437-7315 } E-Mail: jecalvin-at-vassar.edu }
==============================Original Headers============================== 8, 26 -- From dljones-at-bestweb.net Fri Feb 17 15:21:32 2006 8, 26 -- Received: from smtp2.bestweb.net (smtp2-2.bestweb.net [209.94.103.46]) 8, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HLLVkH026422 8, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 15:21:31 -0600 8, 26 -- Received: from smtp2.bestweb.net (localhost [127.0.0.1]) 8, 26 -- by smtp2.bestweb.net (Postfix) with ESMTP id C09831CCF1; 8, 26 -- Fri, 17 Feb 2006 16:21:30 -0500 (EST) 8, 26 -- Received: from dlj (pool-71-249-9-96.nycmny.east.verizon.net [71.249.9.96]) 8, 26 -- by smtp2.bestweb.net (Postfix) with ESMTP id 27CFB1CCE0; 8, 26 -- Fri, 17 Feb 2006 16:21:30 -0500 (EST) 8, 26 -- Date: Fri, 17 Feb 2006 16:30:47 -0500 (Eastern Standard Time) 8, 26 -- From: "David L. Jones" {dljones-at-bestweb.net} 8, 26 -- To: Jerry Calvin {jecalvin-at-vassar.edu} 8, 26 -- cc: Microscopy-at-microscopy.com 8, 26 -- Subject: Re: [Microscopy] Microscope cooling lines 8, 26 -- In-Reply-To: {a06001203c01bde1ab81a-at-[143.229.41.193]} 8, 26 -- Message-ID: {Pine.WNT.4.62.0602171608170.1080-at-dlj} 8, 26 -- References: {200602171946.k1HJkq7E000347-at-ns.microscopy.com} 8, 26 -- {a06001203c01bde1ab81a-at-[143.229.41.193]} 8, 26 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 8, 26 -- MIME-Version: 1.0 8, 26 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed 8, 26 -- X-Spam-Checker-Version: SpamAssassin 3.0.2 (2004-11-16) on smtp2-1.bestweb.net 8, 26 -- X-Spam-Level: 8, 26 -- X-Spam-Status: No, score=-2.8 required=5.0 tests=ALL_TRUSTED autolearn=failed 8, 26 -- version=3.0.2 ==============================End of - Headers==============================
A colleague asked me why she is so much more aware of the floaters in her eyes when she is using the microscope (or the telescope) than at other times. I assured her that it wasn't just her, it happens to a lot of us. But why floaters are so much more noticeable when looking in the microscope I do not know? Any suggestions?
Bob -- C. Robert Bagnell, Jr., Ph.D. Professor and Director, Microscopy Services Laboratory Department of Pathology and Laboratory Medicine University of North Carolina at Chapel Hill Chapel Hill, NC 27599 phone 919-966-2413 fax 919-966-6718 e-mail bagnell-at-med.unc.edu web http://www.med.unc.edu/microscopy
==============================Original Headers============================== 2, 21 -- From bagnell-at-med.unc.edu Fri Feb 17 16:01:56 2006 2, 21 -- Received: from smtp-mx.med.unc.edu (fletcher.med.unc.edu [152.19.4.46]) 2, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HM1u7m008604 2, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:01:56 -0600 2, 21 -- Received: from castlehayne.med.unc.edu (castlehayne.med.unc.edu [152.19.4.29]) 2, 21 -- by smtp-mx.med.unc.edu (8.13.4+Sun/8.13.4) with ESMTP id k1HM1qc1025249 2, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 17:01:56 -0500 (EST) 2, 21 -- Received: from [152.19.80.19] by msrv0.med.unc.edu 2, 21 -- (Sun Java System Messaging Server 6.2-5.02 (built Dec 1 2005)) 2, 21 -- with ESMTPA id {0IUU00C9DQJ236B0-at-msrv0.med.unc.edu} for 2, 21 -- microscopy-at-microscopy.com; Fri, 17 Feb 2006 17:01:51 -0500 (EST) 2, 21 -- Date: Fri, 17 Feb 2006 17:01:49 -0500 2, 21 -- From: Robert Bagnell {bagnell-at-med.unc.edu} 2, 21 -- Subject: Floaters 2, 21 -- X-Sender: rml-at-imap-ns.med.unc.edu 2, 21 -- To: microscopy-at-microscopy.com 2, 21 -- Message-id: {p06110400c01bdabc3b81-at-[152.19.80.19]} 2, 21 -- MIME-version: 1.0 2, 21 -- Content-type: text/plain; charset=us-ascii; format=flowed 2, 21 -- Content-transfer-encoding: 7BIT 2, 21 -- X-Scanned-By: UNC/SoM/OIS/Mail_Filter on 152.19.4.46 ==============================End of - Headers==============================
My service engineer recommends neither tap nor distilled water, but rather bottled spring water. Has anyone yet mentioned the possibility that green sludge in the filter might be algae growing in the cooling water or the cooling unit? --Jan Factor
--------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology --------------------------------------- Natural Sciences Purchase College, State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA --------------------------------------- Office Tel: 914-251-6659 Office Fax: 914-251-6635 E-mail: jfactor-at-ns.purchase.edu or- jan.factor-at-purchase.edu ---------------------------------------
==============================Original Headers============================== 4, 16 -- From jfactor-at-ns.purchase.edu Fri Feb 17 16:02:30 2006 4, 16 -- Received: from zephyr.ns.purchase.edu (zephyr.ns.purchase.edu [199.79.168.193]) 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HM2PAx009529 4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:02:25 -0600 4, 16 -- Received: from [10.52.0.79] ([10.52.0.79]) 4, 16 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) with ESMTP id k1HM5lr17594; 4, 16 -- Fri, 17 Feb 2006 17:05:48 -0500 4, 16 -- Message-ID: {43F647F0.1070409-at-ns.purchase.edu} 4, 16 -- Date: Fri, 17 Feb 2006 17:02:24 -0500 4, 16 -- From: Jan Factor {jfactor-at-ns.purchase.edu} 4, 16 -- User-Agent: Thunderbird 1.5 (Windows/20051201) 4, 16 -- MIME-Version: 1.0 4, 16 -- To: microscopy-at-microscopy.com 4, 16 -- Subject: Re: Microscope cooling lines 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Question: Can anybody provide an explanation concerning the refractive index -dependency of the interference colours seen in charts for estimating the thickness of ultrathin sections? The small print in such charts usually reads "valid for refractive index of ca. 1.5", which is fine for methacrylates and similar embedding media, but what type of shift (if significant) would be observed for a lower refractive index, say 1.3? I am not sure whether the usual charts are valid for the interference colours given by cryosections.
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Title-Subject: [Filtered] NTA-nanogold for His tagged protein in EM?
Question: Has anynone had success using NTA-nanogold to disclose the location of His-tagged proteins by electron microscopy? I would like to attach the nanogold this way before injecting the protein into a cell. An alternative would be to apply gold labelled primary anti-His after fixation and permeabilization.
4 Student Scholarships ($500) Available for support for attending and presenting a paper/poster
PARTICLE WORKSHOP 2006
A Joint NIST / Microbeam Analysis Society Special Topics Workshop
Dates: April 24th - 26th 2006
Location: NIST, Gaithersburg, MD
The analysis of microscopic particles represents a measurement challenge in a diverse range of scientific, technological and environmental disciplines. Examples of areas of interest include nanoscale particles as building blocks for nanotechnology, contamination in high technology manufacturing, trace forensic detection for homeland security, and environmental sampling and monitoring. Characterization of particle specimens is complicated by mass, volume and morphological effects that cause well established bulk techniques to break down. In addition, while the measurement types under consideration at this workshop are performed on a particle-by-particle basis, the properties of interest are often statistical measures of populations of similar particles.
This workshop will focus on the challenges that face industrial and governmental application of microscopic particle measurement techniques. The practical tone of the meeting will set by speakers representing various industries and governmental organizations who will provide insight into their particle measurement requirements. These speakers will be followed by sessions that focus on sample preparation and statistical experimental design and established & emerging measurement techniques. Instrumentation to be discussed include SEM/EDS, AEM, TOF/SIMS, optical, FIB and scanned probe. Each of these sessions will be concluded by a round table discussion in which attendees are encouraged to contribute.
Registration Deadline: March 31, 2006
Students interested in applying for one of the 4 student scholarships should immediately contact
Nicholas W. M. Ritchie NIST Microanalysis Research Group 100 Bureau Drive STOP 8371 Gaithersburg, MD 20899-8371 301-975-3929 nicholas.ritchie-at-nist.gov
There is no registration fee: the workshop is free to all attendees. However, space is limited and pre-registration is required by NIST Security for entry onto the NIST campus. This rule is strictly enforced.
Full information at www.cstl.nist.gov/div837/Division/meetings/particleworkshop/particle.htm
==============================Original Headers============================== 9, 24 -- From johnf-at-geology.wisc.edu Fri Feb 17 16:21:54 2006 9, 24 -- Received: from ice.geology.wisc.edu (ice.geology.wisc.edu [144.92.206.14]) 9, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HMLs1o013872 9, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 17 Feb 2006 16:21:54 -0600 9, 24 -- Received: from localhost (localhost [127.0.0.1]) 9, 24 -- by localhost (Postfix) with ESMTP id F0BEE20D01 9, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 17 Feb 2006 16:21:53 -0600 (CST) 9, 24 -- Received: from ice.geology.wisc.edu ([127.0.0.1]) 9, 24 -- by localhost (ice [127.0.0.1]) (amavisd-new, port 10024) with ESMTP 9, 24 -- id 07362-03 for {Microscopy-at-Microscopy.Com} ; 9, 24 -- Fri, 17 Feb 2006 16:21:49 -0600 (CST) 9, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 9, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 24 -- (No client certificate requested) 9, 24 -- by ice.geology.wisc.edu (Postfix) with ESMTP id 9E1EA20D16 9, 24 -- for {Microscopy-at-Microscopy.Com} ; Fri, 17 Feb 2006 16:21:49 -0600 (CST) 9, 24 -- Mime-Version: 1.0 9, 24 -- Message-Id: {p0623091dc01bfc64a0eb-at-[144.92.206.57]} 9, 24 -- Date: Fri, 17 Feb 2006 16:19:32 -0600 9, 24 -- To: Microscopy-at-Microscopy.Com 9, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 9, 24 -- Subject: student scholarships: NIST/MAS Particle Workshop April 2006 9, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 9, 24 -- X-Virus-Scanned: by amavisd-new at geology.wisc.edu ==============================End of - Headers==============================
It has to do with the physics of simple pinhole optics. Essentially, when you are just in the right focal plane, you are doing an "entopic exam" of your eye. You can also reproduce the experiment by putting a small hole (about 1/8") into a piece of cardboard (1/2 of a file folder) and staring through it at a neutral surface (blank wall; sky, if not too bright). Adjust the distance between the card and your eye and, at the right distance, you will see the internal structure.
I've had an interesting array of tiny cataracts for over 25 years and keep track of their position and size using this method.
Hope this is helpful.
Best regards, Barbara Foster
Microscopy/Microscopy Education 313 S Jupiter Rd, Suite 100 Allen, TX 75002 P: 972-954-8011 W: www.MicroscopyEducation.com
P. S. Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.
At 04:05 PM 2/17/2006, bagnell-at-med.unc.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 15, 17 -- From bfoster-at-mme1.com Fri Feb 17 16:31:50 2006 15, 17 -- Received: from 5starpro.com (enterprise.5starpro.com [207.44.136.95]) 15, 17 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1HMVnJe023336 15, 17 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:31:49 -0600 15, 17 -- Received: (qmail 19789 invoked from network); 17 Feb 2006 17:33:30 -0500 15, 17 -- Received: from host57-99.rancor.birch.net (HELO barbsd505.mme1.com) (65.17.57.99) 15, 17 -- by enterprise.5starpro.com with SMTP; 17 Feb 2006 17:33:30 -0500 15, 17 -- Message-Id: {7.0.1.0.0.20060217162833.01fc9ec0-at-mme1.com} 15, 17 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 15, 17 -- Date: Fri, 17 Feb 2006 16:31:54 -0600 15, 17 -- To: bagnell-at-med.unc.edu, Microscopy Listserver {microscopy-at-microscopy.com} 15, 17 -- From: Barbara Foster {bfoster-at-mme1.com} 15, 17 -- Subject: Re: [Microscopy] Floaters 15, 17 -- In-Reply-To: {200602172205.k1HM5BbH017110-at-ns.microscopy.com} 15, 17 -- References: {200602172205.k1HM5BbH017110-at-ns.microscopy.com} 15, 17 -- Mime-Version: 1.0 15, 17 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I had the same sludge problem about 1.5 years ago in a new Haskris chiller. Haskris recommends using only distilled water. That lasted about three months and the slime appeared. It was a combination of algae and small particles. Dumped the water and replaced with new distilled water and Skasol to flush. Then, new distilled water and one half liter of Hexid A4 from Applied Thermal Control Ltd. UK as supplied by SEM service tech. Chiller seized after about three months. Post mortem indicated that the impeller blades failed. Most likely due to misalignment of motor and pump.
Haskris replaced motor and pump assembly. Fluid was drained and replaced with distilled water and ethylene glycol (.5G to 4.5G distilled water). Filters were changed and no problems for about eight months. Liquid is not starting to become darker and small build up of stuff in chiller main filter. It is time to change liquid and filters. Main filter is in the water tank and is spec'd at about 50u. External toilet paper style filter is spec'd at 2u. So both get changed at the same time.
The Haskris unit specifically says to not use automotive antifreeze since it will deteriorate the BUNA N material in the chiller. Some anti freeze contains ethylene glycol. So I'm puzzled by the successful use of distilled water and EG. Perhaps they meant to say not to use 100% antifreeze rather than a diluted mix.
The other factor is that the SEM came with basically transparent water hoses. This is not good since the light gets into them and advances the algae. So this upcoming liquid and filter replacement will include replacing the hoses with opaque ones.
Overall, there are three aspects to be concerned about:
1. chiller guts and pump 2. hoses 3. SEM items that get chilled water (TMP, coils, etc.)
I don't think that there is a single simple answer to this problem since SEMs are different and chillers are different.
gary g.
At 02:04 PM 2/17/2006, you wrote:
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==============================Original Headers============================== 15, 20 -- From gary-at-gaugler.com Fri Feb 17 16:58:51 2006 15, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 15, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1HMwoD3002918 15, 20 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:58:51 -0600 15, 20 -- Received: (qmail 24885 invoked from network); 17 Feb 2006 14:58:50 -0800 15, 20 -- Received: by simscan 1.1.0 ppid: 24881, pid: 24883, t: 0.0890s 15, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 15, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 15, 20 -- by qsmtp3 with SMTP; 17 Feb 2006 14:58:50 -0800 15, 20 -- Message-Id: {6.2.3.4.2.20060217143542.0205ed40-at-mail.calweb.com} 15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 15, 20 -- Date: Fri, 17 Feb 2006 14:58:51 -0800 15, 20 -- To: jfactor-at-ns.purchase.edu 15, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 15, 20 -- Subject: Re: [Microscopy] Re: Microscope cooling lines 15, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 15, 20 -- In-Reply-To: {200602172204.k1HM4d7s015164-at-ns.microscopy.com} 15, 20 -- References: {200602172204.k1HM4d7s015164-at-ns.microscopy.com} 15, 20 -- Mime-Version: 1.0 15, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Just a couple of points I'd like to make w.r.t. your post.
I would not recommend using ethylene glycol as it is toxic. Propylene glycol is non-toxic.
The problem with using "antifreeze" mixtures not intended for use in these kinds of systems is that they do not usually contain the proper additives. Glycol solutions that are not HVAC grade will deteriorate over time through a type of polymerization that will plug things up and render the system inoperable. The resulting deposits thus formed are very inert and to my knowledge no one has ever found a way to clean them so you basically have to replace the chiller system.
HVAC grade polypropylene contains additives to avoid that problem plus inhibitors that stop corrosion of most commercially available materials in piping. That also includes seal materials, but I'm not sure about specifically N-buena seals. I'd have to look that up, but I would think it also compatible being such a commonly used seal material.
Biofouling is quite common in closed loop systems. Using a biocide is usually used in these systems to eliminate this problem.
The original poster to this thread likely is in a location where they have a professional water treatment company taking care of large HVAC systems. Perhaps they should talk with the representative of that company and find out what is being done for chemical treatment of chilled water systems there. They may be able to just get some of the proper chemicals that likely exist on-site already.
I would also like to point out, there is really no reason to go through the expense of using a glycol based system unless there is danger of freezing the coolant for some reason. There are numerous other water treatments that are much less expensive and work very well to keep a closed loop system running well. If there is little to no make up water needed for the closed system, once set-up properly, there is little more to do other than enjoy a clean running system...
dj
On Fri, 17 Feb 2006, gary-at-gaugler.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I had the same sludge problem about 1.5 years ago in } a new Haskris chiller. Haskris recommends using only } distilled water. That lasted about three months and } the slime appeared. It was a combination of algae and } small particles. Dumped the water and replaced with } new distilled water and Skasol to flush. Then, new } distilled water and one half liter of Hexid A4 from } Applied Thermal Control Ltd. UK as supplied by SEM } service tech. Chiller seized after about three months. } Post mortem indicated that the impeller blades failed. } Most likely due to misalignment of motor and pump. } } Haskris replaced motor and pump assembly. Fluid was } drained and replaced with distilled water and ethylene } glycol (.5G to 4.5G distilled water). Filters were } changed and no problems for about eight months. Liquid } is not starting to become darker and small build up of } stuff in chiller main filter. It is time to change liquid and } filters. Main filter is in the water tank and is spec'd } at about 50u. External toilet paper style filter is spec'd } at 2u. So both get changed at the same time. } } The Haskris unit specifically says to not use automotive } antifreeze since it will deteriorate the BUNA N material in } the chiller. Some anti freeze contains ethylene glycol. } So I'm puzzled by the successful use of distilled water and } EG. Perhaps they meant to say not to use 100% antifreeze } rather than a diluted mix. } } The other factor is that the SEM came with basically transparent } water hoses. This is not good since the light gets into them } and advances the algae. So this upcoming liquid and filter } replacement will include replacing the hoses with opaque ones. } } Overall, there are three aspects to be concerned about: } } 1. chiller guts and pump } 2. hoses } 3. SEM items that get chilled water (TMP, coils, etc.) } } I don't think that there is a single simple answer to this } problem since SEMs are different and chillers are different. } } gary g. } } } } At 02:04 PM 2/17/2006, you wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } My service engineer recommends neither tap nor distilled water, but } } rather bottled spring water. Has anyone yet mentioned the possibility } } that green sludge in the filter might be algae growing in the cooling } } water or the cooling unit? } } --Jan Factor } } } } --------------------------------------- } } Jan Robert Factor, Ph.D. } } Professor of Biology } } --------------------------------------- } } Natural Sciences } } Purchase College, State University of New York } } 735 Anderson Hill Rd. } } Purchase, NY 10577 } } USA } } --------------------------------------- } } Office Tel: 914-251-6659 } } Office Fax: 914-251-6635 } } E-mail: jfactor-at-ns.purchase.edu } } or- jan.factor-at-purchase.edu } } --------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 4, 16 -- From jfactor-at-ns.purchase.edu Fri Feb 17 16:02:30 2006 } } 4, 16 -- Received: from zephyr.ns.purchase.edu } } (zephyr.ns.purchase.edu [199.79.168.193]) } } 4, 16 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } } k1HM2PAx009529 } } 4, 16 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 } } 16:02:25 -0600 } } 4, 16 -- Received: from [10.52.0.79] ([10.52.0.79]) } } 4, 16 -- by zephyr.ns.purchase.edu (AIX5.1/8.11.6p2/8.11.0) } } with ESMTP id k1HM5lr17594; } } 4, 16 -- Fri, 17 Feb 2006 17:05:48 -0500 } } 4, 16 -- Message-ID: {43F647F0.1070409-at-ns.purchase.edu} } } 4, 16 -- Date: Fri, 17 Feb 2006 17:02:24 -0500 } } 4, 16 -- From: Jan Factor {jfactor-at-ns.purchase.edu} } } 4, 16 -- User-Agent: Thunderbird 1.5 (Windows/20051201) } } 4, 16 -- MIME-Version: 1.0 } } 4, 16 -- To: microscopy-at-microscopy.com } } 4, 16 -- Subject: Re: Microscope cooling lines } } 4, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 4, 16 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 15, 20 -- From gary-at-gaugler.com Fri Feb 17 16:58:51 2006 } 15, 20 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) } 15, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1HMwoD3002918 } 15, 20 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 16:58:51 -0600 } 15, 20 -- Received: (qmail 24885 invoked from network); 17 Feb 2006 14:58:50 -0800 } 15, 20 -- Received: by simscan 1.1.0 ppid: 24881, pid: 24883, t: 0.0890s } 15, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 } 15, 20 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) } 15, 20 -- by qsmtp3 with SMTP; 17 Feb 2006 14:58:50 -0800 } 15, 20 -- Message-Id: {6.2.3.4.2.20060217143542.0205ed40-at-mail.calweb.com} } 15, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 } 15, 20 -- Date: Fri, 17 Feb 2006 14:58:51 -0800 } 15, 20 -- To: jfactor-at-ns.purchase.edu } 15, 20 -- From: Gary Gaugler {gary-at-gaugler.com} } 15, 20 -- Subject: Re: [Microscopy] Re: Microscope cooling lines } 15, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} } 15, 20 -- In-Reply-To: {200602172204.k1HM4d7s015164-at-ns.microscopy.com} } 15, 20 -- References: {200602172204.k1HM4d7s015164-at-ns.microscopy.com} } 15, 20 -- Mime-Version: 1.0 } 15, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 21 -- From "dljones-at-bestweb.net"-at-bestweb.net Fri Feb 17 20:43:13 2006 12, 21 -- Received: from mta4.srv.hcvlny.cv.net (mta4.srv.hcvlny.cv.net [167.206.4.199]) 12, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1I2hDlf008914 12, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 20:43:13 -0600 12, 21 -- Received: from localhost (ool-44c62883.dyn.optonline.net [68.198.40.131]) 12, 21 -- by mta4.srv.hcvlny.cv.net 12, 21 -- (Sun Java System Messaging Server 6.2-4.03 (built Sep 22 2005)) 12, 21 -- with ESMTP id {0IUV00D2N3JYUD00-at-mta4.srv.hcvlny.cv.net} for 12, 21 -- microscopy-at-microscopy.com; Fri, 17 Feb 2006 21:43:13 -0500 (EST) 12, 21 -- Date: Fri, 17 Feb 2006 21:43:12 -0500 (Eastern Standard Time) 12, 21 -- From: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net} 12, 21 -- Subject: Re: [Microscopy] Microscope cooling lines 12, 21 -- In-reply-to: {200602172305.k1HN5bhk012207-at-ns.microscopy.com} 12, 21 -- X-X-Sender: dljones-at-pop-croton.bestweb.net 12, 21 -- To: gary-at-gaugler.com 12, 21 -- Cc: microscopy-at-microscopy.com 12, 21 -- Message-id: {Pine.WNT.4.64.0602172109370.408-at-dljtoshiba} 12, 21 -- MIME-version: 1.0 12, 21 -- Content-type: TEXT/PLAIN; charset=US-ASCII; format=flowed 12, 21 -- Content-transfer-encoding: 7BIT 12, 21 -- References: {200602172305.k1HN5bhk012207-at-ns.microscopy.com} ==============================End of - Headers==============================
If EG is toxic and PG is not, that is a factor. However, I do not care if one or the other is toxic. I do not swim in the 5 gallons of fluid. So, between the two glycols, which is best for SEM? I do not know. What do you think?
So there are toxic issues, corrosive issues, etc., etc. Well, then just dump the SEM, eh? No. I do not think so. One must work with the materials at hand. So, what do you think are the best materials for chiller fluid?
gary g.
At 06:43 PM 2/17/2006, you wrote: } Gary, } } Just a couple of points I'd like to make w.r.t. your post. } } I would not recommend using ethylene glycol as it is toxic. } Propylene glycol is non-toxic. } } The problem with using "antifreeze" mixtures not intended for use in } these kinds of systems is that they do not usually contain the } proper additives. Glycol solutions that are not HVAC grade will } deteriorate over time through a type of polymerization that will } plug things up and render the system inoperable. The resulting } deposits thus formed are very inert and to my knowledge no one has } ever found a way to clean them so you basically have to replace the } chiller system. } } HVAC grade polypropylene contains additives to avoid that problem } plus inhibitors that stop corrosion of most commercially available } materials in piping. That also includes seal materials, but I'm not } sure about specifically N-buena seals. I'd have to look that up, but } I would think it also compatible being such a commonly used seal material. } } Biofouling is quite common in closed loop systems. Using a biocide } is usually used in these systems to eliminate this problem. } } The original poster to this thread likely is in a location where } they have a professional water treatment company taking care of } large HVAC systems. Perhaps they should talk with the representative } of that company and find out what is being done for chemical } treatment of chilled water systems there. They may be able to just } get some of the proper chemicals that likely exist on-site already. } } I would also like to point out, there is really no reason to go } through the expense of using a glycol based system unless there is } danger of freezing the coolant for some reason. There are numerous } other water treatments that are much less expensive and work very } well to keep a closed loop system running well. If there is little } to no make up water needed for the closed system, once set-up } properly, there is little more to do other than enjoy a clean running system... } } dj } } On Fri, 17 Feb 2006, gary-at-gaugler.com wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 21 -- From gary-at-gaugler.com Fri Feb 17 20:51:47 2006 9, 21 -- Received: from qsmtp3.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1I2pkQd018305 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 17 Feb 2006 20:51:47 -0600 9, 21 -- Received: (qmail 22693 invoked from network); 17 Feb 2006 18:51:46 -0800 9, 21 -- Received: by simscan 1.1.0 ppid: 22690, pid: 22691, t: 0.2511s 9, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 21 -- Received: from unknown (HELO ivan.gaugler.com) (66.60.171.211) 9, 21 -- by qsmtp3 with SMTP; 17 Feb 2006 18:51:46 -0800 9, 21 -- Message-Id: {6.2.3.4.2.20060217184632.02068418-at-mail.calweb.com} 9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 6.2.3.4 9, 21 -- Date: Fri, 17 Feb 2006 18:51:46 -0800 9, 21 -- To: "David L. Jones" {"dljones-at-bestweb.net"-at-bestweb.net} 9, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 21 -- Subject: Re: [Microscopy] Microscope cooling lines 9, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 21 -- In-Reply-To: {Pine.WNT.4.64.0602172109370.408-at-dljtoshiba} 9, 21 -- References: {200602172305.k1HN5bhk012207-at-ns.microscopy.com} 9, 21 -- {Pine.WNT.4.64.0602172109370.408-at-dljtoshiba} 9, 21 -- Mime-Version: 1.0 9, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This is a perennial problem, I know, but does anyone have any effective "pet" solutions to the problem of charging and subsequent electrostatic arcing of TEM film in the extremely dry air we get in New England typically in the winter, and some other people get at other seasons of the year?
The discharges can occur at almost any stage of the handling, from getting the film out of the vendor's packing, loading and unloading in the microscope carriers and loading in the developing racks (we use Lucite ones). We have considered a humidifier in the darkroom, but that would involve blocking off the ventilation (we have make-up air positively blown into to room and exhaust actively sucked out) to prevent our moisture from being simply blown away.
Any hints would be welcome, but switching to digital imaging is one that is not going to happen.
Tony.
************************************* Anthony J. Garratt-Reed M.A. D.Phil. MIT Room #13-1027 77 Massachusetts Avenue Cambridge, Massachusetts 02139-4307 USA
Below are responses (abbreviated) to my question about which to use, copper or PVC, to replace water lines running from cooling units to microscopes. I will run any suggestions by my service engineers just to make sure there are no concerns regarding specific equipment. I do know that water pH is important and that may play a role in determining what additives to use:
I am a service engineer who works on electron microscopes for the last 17 years and my point of view: I would go with copper this way no matter what may or may not happen to the lines (cleaning out - whatever) you have no worries. (Ray Spengler)
I do not think that there will be a problem using copper or PVC. But you chould check with the eq maker to see if they have any issues. We use Copper and have not had any problems. We do get build up over time due to the hoses "rotting" over time and general scale buildup. (Karl Weiss)
USE PVC and make the final connection to the equipment with two coils of rubber hose for vibration control. ( Fran Laabs)
For our new IMAGE building, I specified PVC piping for the closed circ loop between the water chillers and scopes. We still notice a greenish sludge building up in the water filters (takes about 6-8 months to become significant) but I am certain that this is coming from the EM (copper cooling coils and iron connections---} electrolytic reaction). The EM service people told us that if we ever used acid to clean the lines that they would no longer warranty the microscope. The PVC lines are perfectly clean. (J. Bozzola)
Another material you may wish to consider is called PEX, which is a cross-linked polyethylene. I don't know too much about its characteristics, except that it's very smooth inside, which should retard crud accumulation and it's more opaque than white pvc. It may be worth checking out. (P. Grover)
As an FYI, if you have copper based and iron based materials mixed in the same system, the iron will corrode preferentially through galvanic coupling, not the copper. In fact, the iron becomes a sacrificial anode protecting the copper from corrosion. Any time you have those two materials in the same system, you should have dielectric couplings between the two or you will actively corrode the ferrous based material. Regarding acid cleaning, you should know what your deposits are in your piping before deciding how to clean them. As an FYI, copper will generally corrode at pH's below about 6.3 or so. There are low pH cleaners that can be used with copper, but they contain corrosion inhibitors. (D. Jones)
If you intend to clean the lines with acid, I suggest PVC, since Cu can be etched at low pH. In addition to floating some dichlorophene for algal control, we add a corrosion inhibitor. We have been using a Mo-based formula, which was available from Aqua Labs on the East coast and from Skasol on the West coast, so find a distributor in your area. I think that either Aqua or Skasol would be able to give you that info.(B. Tivol)
Along these lines, I noticed a lot more copper leaching into the lines when I used deionized water than regular or filtered tap water. Secondly, Why hasn't anyone suggested using antifreeze? Antifreeze intended for cars should have corrosion inhibitors and should be suitable for this purpose. I have still gotten green particles regardless of whether antifreeze was used or not and have regularly made it a practice to clean the filters in the lines once a semester. (Jerry Calvin)
I have used the ethylene/glycol/water mix through clear PVC piping between my circulator and TEM and vacuum coating unit for six years now. There is a long run of the piping in daylight. Absolutely no algae Problem. Use 50/50 ethylene glycol*/water and PVC pipes and you will never have a problem again. Make sure that the chiller/recirculator manufacturer OKs the use of ethylene glycol but I wouldn't anticipate any problem. Even 20/80 EG/Water will work well. (Ted Dunn)
Debby
Debby Sherman, Manager Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www3.agriculture.purdue.edu/microscopy
==============================Original Headers============================== 17, 21 -- From dsherman-at-purdue.edu Sat Feb 18 10:40:08 2006 17, 21 -- Received: from exchange.purdue.edu (1061exfe02.adpc.purdue.edu [128.210.63.223]) 17, 21 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1IGe8dl014778 17, 21 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 10:40:08 -0600 17, 21 -- Received: from EXCH02.purdue.lcl ([128.210.63.234]) by exchange.purdue.edu with Microsoft SMTPSVC(6.0.3790.211); 17, 21 -- Sat, 18 Feb 2006 11:40:08 -0500 17, 21 -- Received: from 128.210.161.94 ([128.210.161.94]) by EXCH02.purdue.lcl ([128.210.63.237]) via Exchange Front-End Server exch.itap.purdue.edu ([128.210.63.239]) with Microsoft Exchange Server HTTP-DAV ; 17, 21 -- Sat, 18 Feb 2006 16:40:08 +0000 17, 21 -- User-Agent: Microsoft-Entourage/11.2.1.051004 17, 21 -- Date: Sat, 18 Feb 2006 11:40:07 -0500 17, 21 -- Subject: Cooling lines-responses 17, 21 -- From: Debby Sherman {dsherman-at-purdue.edu} 17, 21 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 17, 21 -- Message-ID: {C01CB817.D64C%dsherman-at-purdue.edu} 17, 21 -- Thread-Topic: Cooling lines-responses 17, 21 -- Thread-Index: AcY0qfmyOEGiJKCdEdqwBAARJN08Mg== 17, 21 -- Mime-version: 1.0 17, 21 -- Content-type: text/plain; 17, 21 -- charset="US-ASCII" 17, 21 -- Content-transfer-encoding: 7bit 17, 21 -- X-OriginalArrivalTime: 18 Feb 2006 16:40:08.0197 (UTC) FILETIME=[FA697350:01C634A9] ==============================End of - Headers==============================
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Email: soleimanij-at-tbzmed.ac.ir Name: Jafar Soleimani Rad
Organization: University
Title-Subject: [Filtered] scale bar
Question: Hi we are using A LEO 906 TEM, scale bar is not registered in the microfilms. We are having problem to calculating the real sizes. any help in this matter is appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rpowell-at-nanoprobes.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rpowell-at-nanoprobes.com Name: Rick Powell
Organization: Nanoprobes, Incorporated
Title-Subject: [Filtered] Re: [Microscopy]: NTA-nanogold for His tagged protein in EM?
Question: [Commercial disclaimer - I work for Nanoprobes, and we make NTA-Ni(II)-NanogoldĘ]
Hello Milton:
Here are a few recent references for EM using NTA-Ni(II)-Nanogold, with links to articles on our online newsletter that describe them:
(1) Wolfe, C. L.; Warrington, J. A.; Treadwell, L., and Norcum, M. T.: A three-dimensional working model of the multienzyme complex of aminoacyl-tRNA synthetases based on electron microscopic placements of tRNA and proteins. J. Biol. Chem., 280, 38870-38878 (2005).
(2) Bumba, L.; Tichy, M.; Dobakova, M.; Komenda, J., and Vacha, F.: Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged NińNTA Nanogold labeling. J. Struct. Biol., 152, 28-35 (2005).
There are two more recent references in which the reagent was used to label polyhistidine-tagged components of viral capsids:
(3) Chatterji, A.; Ochoa, W. F.; Ueno, T.; Lin T., and Johnson, J. E.: A virus-based nanoblock with tunable electrostatic properties. Nano Lett., 5, 597-602 (2005).
(4) Collins, R. F.; Frye, S. A.; Balasingham, S.; Ford, R. C.; Tonjum, T., and Derrick, J. P.: Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ. J. Biol. Chem., 280, 18923-18930 (2005).
Details about the reagent are available on our web site:
Catalog and general info: http://www.nanoprobes.com/NTAgold.html
Product info and instructions: http://www.nanoprobes.com/Inf2080.html
We have found that the complexes of NTA-Ni(II)-Nanogold bound to polyhis-tagged proteins hold together well during chromtographic separations (gel filtration), implying that they should be injectable.
Hope some of these are helpful,
Rick Powell Nanoprobes, Inc. www.nanoprobes.com
At 05:09 PM 2/17/2006, you wrote: Question: Has anynone had success using NTA-nanogold to disclose the location of His-tagged proteins by electron microscopy? I would like to attach the nanogold this way before injecting the protein into a cell. An alternative would be to apply gold labelled primary anti-His after fixation and permeabilization.
This Question was submitted to Ask-A-Microscopist by (grmitch-at-netzero.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, February 15, 2006 at 20:08:01 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both grmitch-at-netzero.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: grmitch-at-netzero.com Name: Linda Mitchell
Organization: BPS Environmental Center
Education: K-8 Grade Grammar School
Location: Birmingham, Michigan USA
Title: Microorganisms!
Question: I will be teaching a unit on microscopic life to 5th graders next month, March (quite a cold month here in Michigan). My question for you: What is the best way to keep my microorganisms alive throughout the program? We obtain our samples directly from the 10 acres of the property here at the nature center. One of the samples is from our pond area and the second from the swamp woods area. We have no difficulty in finding a healthy sample, yet the problem that we often encounter is keeping them alive. We have supplies - lab pans, microscopes, even an aquarium that is our "indoor pond" when the water ices over. Do you have any feeding, climate tips that would help in these areas? This is a program that is enjoyed by both the staff and students - they are so amazed by what they find. Thanks for your input.
Millennium Chemicals (a Lyondell Company), the world's second largest producer of Titanium Dioxide, is seeking a microscopist with experience in the areas of Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM) and X-ray Diffraction (XRD) to support R&D, Commercial and Manufacturing activities at its Baltimore Research Center.
EDUCATIONAL REQUIREMENTS
Minimum M.S. in chemistry, mineralogy, material science or similar field, with 7-10 years experience in an industrial R&D environment preferred.
DESCRIPTION:
The primary responsibility of the position is the application of advanced microscopic techniques to investigate properties, mineralogy, phase distribution, morphology and structure/function relationships of pigmentary and catalytic TiO2 particles, catalysts and polymers. In addition to conducting research and and support activities, the candidate will be responsible for oversight and maintenance of a state-of-the-art microscopy laboratory that includes:
*Olympus SZX7 stereoscope *Olympus BX51 Optical Light Microscope *Amray 1930 SEM with EDS (scheduled for replacement in 2006) *Jeol 2000 FX2 with EDS and STEM *Veeco Nanoscope 3A Dimension 3100 AFM *Panalytical X'pert Pro XRD *RMC PT-X Ultramicrotome with CR-X Cryosectioning system *Gatan Model 691 Precision Ion Polishing System *SPE Plasma Prep II Plasma Etcher/Asher *Denton sputterer/coater
SALARY
Salary is commensurate with education and experience. Millennium Chemicals (A Lyondell Company) offers a competitive benefits package including relocation.
For more information and to submit a resume online, please visit our website:
Millennium Chemicals (a Lyondell Company) is an Equal Opportunity Employer M/F/D/V
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==============================Original Headers============================== 20, 24 -- From Laurine.Ottmar-at-millenniumchem.com Mon Feb 20 06:02:30 2006 20, 24 -- Received: from edcpap01.lyo.com (mail3.lyondell.com [161.16.0.77]) 20, 24 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KC2Ucr017418 20, 24 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 06:02:30 -0600 20, 24 -- Received: from edcexp02.lyondell.com ([161.16.33.40]) 20, 24 -- by edcpap01.lyo.com (8.13.4/8.13.4) with ESMTP id k1KC2Nb9003328 20, 24 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 06:02:23 -0600 20, 24 -- Received: from amnhhtv01.millenniumchem.com ([172.19.0.46]) by edcexp02.lyondell.com with Microsoft SMTPSVC(5.0.2195.6713); 20, 24 -- Mon, 20 Feb 2006 06:02:24 -0600 20, 24 -- Subject: Employment Opportunity 20, 24 -- To: microscopy-at-microscopy.com 20, 24 -- X-Mailer: Lotus Notes Release 5.0.11 July 24, 2002 20, 24 -- Message-ID: {OF1550363E.97FA6230-ON8525711A.006B77CF-8525711B.0042227A-at-millenniumchem.com} 20, 24 -- From: Laurine.Ottmar-at-millenniumchem.com 20, 24 -- Date: Mon, 20 Feb 2006 07:02:22 -0500 20, 24 -- X-MIMETrack: Serialize by Router on AMNHHTV01/HUB/SRV/MIC(Release 5.0.11 |July 24, 2002) at 20, 24 -- 02/20/2006 07:02:24 AM 20, 24 -- MIME-Version: 1.0 20, 24 -- Content-type: text/plain; charset=iso-8859-1 20, 24 -- X-OriginalArrivalTime: 20 Feb 2006 12:02:24.0991 (UTC) FILETIME=[83317EF0:01C63615] 20, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 mlx=0 adultscore=0 adjust=0 reason=mlx engine=3.1.0-06021401 definitions=3.0.0-06022000 20, 24 -- X-Proofpoint-OriginalSender: Laurine.Ottmar-at-millenniumchem.com 20, 24 -- Content-Transfer-Encoding: 8bit 20, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KC2Ucr017418 ==============================End of - Headers==============================
one thing that occurs to me is that you will probably have vinyl flooring in your darkroom. This may be a major source of static and it may be possible to get some advice on low static flooring. I know we had some installed in one of our microscope cubicles and it didn't seem to be tremendously expensive at the time.
If you think that the floor is part of the problem then it might be worth checking whether the soles of your shoes are man made (create static) or leather.
If you want to go for a tried and tested electronics industry route look for ionisers (rather than de-ionisers) I did a simple Google search on ioniser and static and came up with a few using keywords "ionisers static" such as http://www.buystatic.com/static_eliminators.htm?ioniser.htm
I was hoping that you could get away with a domestic ioniser - the sort they sell to simulate sea or mountain air and supposedly gets rid of headaches. But apparently they aren't much good at eliminating static whereas the industrial ones are.
Good luck
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK
e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: tonygr-at-MIT.EDU
I have a friend who seems particularly prone to this even when the humidity is not very low - she can produce amazing patterns of sparks and spirals on a negative simply by waving her hands over them!
The simple way of getting rid of electrostatic problems in a semiconductor company like mine is to 'borrow' an earth strap and mat from the test area. It solved the problem completely. I guess you may have to buy them..
} ------------------------------------------------------------------- } --------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } AmericaTo Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserverOn-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html------------ } ---------------------------------------------------------------- } } This is a perennial problem, I know, but does anyone have any } effective "pet" solutions to the problem of charging and } subsequent electrostatic arcing of TEM film in the extremely dry } air we get in New England typically in the winter, and some other } people get at other seasons of the year? } } The discharges can occur at almost any stage of the handling, from } getting the film out of the vendor's packing, loading and } unloading in the microscope carriers and loading in the developing } racks (we use Lucite ones). We have considered a humidifier in } the darkroom, but that would involve blocking off the ventilation } (we have make-up air positively blown into to room and exhaust } actively sucked out) to prevent our moisture from being simply } blown away. } } Any hints would be welcome, but switching to digital imaging is } one that is not going to happen. } } Tony. } } ************************************* } Anthony J. Garratt-Reed M.A. D.Phil. } MIT Room #13-1027 } 77 Massachusetts Avenue } Cambridge, Massachusetts 02139-4307 } USA } } Tel: (617) 253-4622 } Fax: (617) 258-6478 } ************************************* } } } ==============================Original } Headers==============================7, 26 -- From tonygr-at-MIT.EDU } Sat Feb 18 07:54:11 2006 } 7, 26 -- Received: from biscayne-one-station.mit.edu (BISCAYNE-ONE- } STATION.MIT.EDU [18.7.7.80]) } 7, 26 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id } k1IDsB7m0033107, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 } Feb 2006 07:54:11 -0600 } 7, 26 -- Received: from outgoing.mit.edu (OUTGOING-AUTH.MIT.EDU } [18.7.22.103])7, 26 -- by biscayne-one-station.mit.edu } (8.12.4/8.9.2) with ESMTP id k1IDp3gq004012 } 7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 } 08:51:03 -0500 (EST) } 7, 26 -- Received: from [192.168.1.47] (pool-72-70-109- } 28.bstnma.east.verizon.net [72.70.109.28]) } 7, 26 -- (authenticated bits=0) } 7, 26 -- (User authenticated as tonygr-at-ATHENA.MIT.EDU) } 7, 26 -- by outgoing.mit.edu (8.13.1/8.12.4) with ESMTP id } k1IDosBT0052947, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA- AES256- } SHA bits=256 verify=NOT) } 7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2006 } 08:51:01 -0500 (EST) } 7, 26 -- Message-ID: {43F7263D.9010808-at-mit.edu} } 7, 26 -- Date: Sat, 18 Feb 2006 08:50:53 -0500 } 7, 26 -- From: Tony Garratt-Reed {tonygr-at-MIT.EDU} } 7, 26 -- User-Agent: Thunderbird 1.5 (Windows/20051201) } 7, 26 -- MIME-Version: 1.0 } 7, 26 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} } 7, 26 -- Subject: TEM film electrostatic discharging } 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 26 -- Content-Transfer-Encoding: 7bit } 7, 26 -- X-Spam-Score: 1.217 } 7, 26 -- X-Spam-Level: * (1.217) } 7, 26 -- X-Spam-Flag: NO } 7, 26 -- X-Scanned-By: MIMEDefang 2.42 } ==============================End of - } Headers==============================
==============================Original Headers============================== 10, 35 -- From malcolm.haswell-at-sunderland.ac.uk Mon Feb 20 09:22:15 2006 10, 35 -- Received: from max1.sunderland.ac.uk (max1.sunderland.ac.uk [157.228.29.83]) 10, 35 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1KFMEwG029654 10, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 09:22:15 -0600 10, 35 -- Received: (qmail 10493 invoked from network); 20 Feb 2006 15:22:10 -0000 10, 35 -- Received: from localhost (127.0.0.1) 10, 35 -- by max1.sunderland.ac.uk with SMTP; 20 Feb 2006 15:22:10 -0000 10, 35 -- Received: from max1.sunderland.ac.uk ([127.0.0.1]) 10, 35 -- by localhost (max1.sunderland.ac.uk [127.0.0.1]) (amavisd-new, port 10024) 10, 35 -- with SMTP id 09230-09 for {Microscopy-at-microscopy.com} ; 10, 35 -- Mon, 20 Feb 2006 15:22:06 +0000 (GMT) 10, 35 -- Received: (qmail 10462 invoked by uid 516); 20 Feb 2006 15:22:06 -0000 10, 35 -- Received: from [157.228.37.117] (HELO hermes.sunderland.ac.uk) (157.228.37.117) 10, 35 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Mon, 20 Feb 2006 15:22:06 +0000 10, 35 -- Received: from sunderland.ac.uk (localhost [127.0.0.1]) 10, 35 -- by hermes.sunderland.ac.uk 10, 35 -- (iPlanet Messaging Server 5.2 Patch 2 (built Jul 14 2004)) 10, 35 -- with ESMTP id {0IUZ00MBGS0VEP-at-hermes.sunderland.ac.uk} for 10, 35 -- Microscopy-at-microscopy.com; Mon, 20 Feb 2006 15:22:07 +0000 (GMT) 10, 35 -- Received: from [157.228.15.253] by hermes.sunderland.ac.uk (mshttpd); Mon, 10, 35 -- 20 Feb 2006 15:22:07 +0000 (GMT) 10, 35 -- Date: Mon, 20 Feb 2006 15:22:07 +0000 (GMT) 10, 35 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} 10, 35 -- Subject: Re: [Microscopy] TEM film electrostatic discharging 10, 35 -- To: tonygr-at-MIT.EDU, Microscopy MSA {Microscopy-at-microscopy.com} 10, 35 -- Message-id: {b19b04b19e96.b19e96b19b04-at-sunderland.ac.uk} 10, 35 -- MIME-version: 1.0 10, 35 -- X-Mailer: iPlanet Messenger Express 5.2 Patch 2 (built Jul 14 2004) 10, 35 -- Content-type: text/plain; charset=us-ascii 10, 35 -- Content-language: en 10, 35 -- Content-transfer-encoding: 7BIT 10, 35 -- Content-disposition: inline 10, 35 -- X-Accept-Language: en 10, 35 -- Priority: normal 10, 35 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk ==============================End of - Headers==============================
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==============================Original Headers============================== 8, 31 -- From richard.beanland-at-bookham.com Mon Feb 20 10:38:12 2006 8, 31 -- Received: from mail78.messagelabs.com (mail78.messagelabs.com [195.245.230.131]) 8, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1KGcBCj008019 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 10:38:11 -0600 8, 31 -- X-VirusChecked: Checked 8, 31 -- X-Env-Sender: richard.beanland-at-bookham.com 8, 31 -- X-Msg-Ref: server-4.tower-78.messagelabs.com!1140453489!7816629!1 8, 31 -- X-StarScan-Version: 5.5.9.1; banners=bookham.com,-,- 8, 31 -- X-Originating-IP: [213.249.209.179] 8, 31 -- Received: (qmail 28862 invoked from network); 20 Feb 2006 16:38:09 -0000 8, 31 -- Received: from unknown (HELO cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI) (213.249.209.179) 8, 31 -- by server-4.tower-78.messagelabs.com with SMTP; 20 Feb 2006 16:38:09 -0000 8, 31 -- Received: from cas-smx-02.BOOKHAM.ENTERPRISE.PRI ([10.4.0.223]) by cas-smx-brdg-01.BOOKHAM.ENTERPRISE.PRI with Microsoft SMTPSVC(6.0.3790.211); 8, 31 -- Mon, 20 Feb 2006 16:38:08 +0000 8, 31 -- X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 8, 31 -- Content-class: urn:content-classes:message 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; 8, 31 -- charset="us-ascii" 8, 31 -- Subject: Re: TEM film electrostatic discharging 8, 31 -- Date: Mon, 20 Feb 2006 16:38:08 -0000 8, 31 -- Message-ID: {9645D3E33E4C6548B12A7B25F611533E028190-at-cas-smx-02.BOOKHAM.ENTERPRISE.PRI} 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- Thread-Topic: TEM film electrostatic discharging 8, 31 -- Thread-Index: AcY2MdESLSSjQkN8S4i066gcnb8TQQACVscg 8, 31 -- From: "Richard Beanland" {richard.beanland-at-bookham.com} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- X-OriginalArrivalTime: 20 Feb 2006 16:38:08.0664 (UTC) FILETIME=[07FDA980:01C6363C] 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KGcBCj008019 ==============================End of - Headers==============================
Advice needed on TEM sample prep for cultured cells.
We are growing skin fibroblasts in culture and would like to examine the cells with transmission electron microscopy. To date, our results have been disappointing. We grew the cells on Thermanox coverslips, fixed for 1 hr at room temp in cacodylate-buffered 1.5% glutaraldehyde, 1.5% paraformaldehyde. After buffer washes, the cells were post-fixed with 1% osmium tetroxide, then washed, dehydrated in acetone and embedded in Epon/Araldite.
The cells look OK in general, but the membranes (both plasma membrane and internal membranes) are all rather fuzzy and indistinct. The quality of the ultrastructure is much poorer that we obtain with tissues prepared using almost identical proceures. One would think that since the cells are only a monolayer, preservation would be excellent.
If you have experience with TEM of cultured cells and have obtained good results, I would appreciate any advice you can give me to get better quality ultrastructure.
Please respond to me directly at katzm-at-health.missouri.edu
Thanks,
Martin L. Katz, Ph.D. Professor Ophthalmology, Pathobiology, Neurosciences, Genetics Mason Eye Institute University of Missouri School of Medicine Columbia, Missouri 65212 Phone (573) 882-8480 FAX (573) 884-4100 katzm-at-health.missouri.edu http://www.muhealth.org/~ophthalmology/Katz.htm
==============================Original Headers============================== 9, 23 -- From TindallR-at-missouri.edu Mon Feb 20 13:19:57 2006 9, 23 -- Received: from um-exproto9.um.umsystem.edu (um-exproto9.um.umsystem.edu [207.160.151.49]) 9, 23 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KJJv9B019566 9, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 13:19:57 -0600 9, 23 -- Received: from UM-EMAIL09.um.umsystem.edu ([207.160.151.31]) by um-exproto9.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.1830); 9, 23 -- Mon, 20 Feb 2006 13:19:57 -0600 9, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 23 -- Content-class: urn:content-classes:message 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: text/plain; 9, 23 -- charset="iso-8859-1" 9, 23 -- Subject: TEM: Cell culture preparation 9, 23 -- Date: Mon, 20 Feb 2006 13:19:56 -0600 9, 23 -- Message-ID: {BA876152E8653240BE8572E897083EE701849D7E-at-UM-EMAIL09.um.umsystem.edu} 9, 23 -- X-MS-Has-Attach: 9, 23 -- X-MS-TNEF-Correlator: 9, 23 -- Thread-Topic: TEM: Cell culture preparation 9, 23 -- Thread-Index: AcY2UqHzjBChwr/YROWHPWjAaQBpkg== 9, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 9, 23 -- To: {microscopy-at-microscopy.com} 9, 23 -- X-OriginalArrivalTime: 20 Feb 2006 19:19:57.0367 (UTC) FILETIME=[A2D42C70:01C63652] 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KJJv9B019566 ==============================End of - Headers==============================
You should be able to dispense with the formalin (not "paraformaldehyde" -- that's the solid from which fresh formalin is made). Monolayers of cells generally do not need formalin. Add 1% tannic acid to both the glut and osmium. Mallinckrodt 1764; the monomeric form seems to work better. You may only need the tannic acid in one of the fixes, probably the glut, but putting it in both the fixes won't hurt. You may need to fix for 2 hours, but one hour should be enough. Note: this is for high-resolution SEM, where any holes in the plasma membrane are blatant, and it works well up to mags } 100kX (and higher). Also, you could try growing the cells on glass coverslips, and digesting off the coverslip with HF after fixation, but that's probably not relevant to your problem. Good luck.
Phil
} Advice needed on TEM sample prep for cultured cells. } } We are growing skin fibroblasts in culture and } would like to examine the cells with } transmission electron microscopy. To date, our } results have been disappointing. We grew the } cells on Thermanox coverslips, fixed for 1 hr at } room temp in cacodylate-buffered 1.5% } glutaraldehyde, 1.5% paraformaldehyde. After } buffer washes, the cells were post-fixed with 1% } osmium tetroxide, then washed, dehydrated in } acetone and embedded in Epon/Araldite. } } The cells look OK in general, but the membranes } (both plasma membrane and internal membranes) } are all rather fuzzy and indistinct. The } quality of the ultrastructure is much poorer } that we obtain with tissues prepared using } almost identical proceures. One would think } that since the cells are only a monolayer, } preservation would be excellent. } } If you have experience with TEM of cultured } cells and have obtained good results, I would } appreciate any advice you can give me to get } better quality ultrastructure. } } Please respond to me directly at katzm-at-health.missouri.edu } } Thanks, } } Martin L. Katz, Ph.D. } Professor } Ophthalmology, Pathobiology, Neurosciences, Genetics } Mason Eye Institute } University of Missouri School of Medicine } Columbia, MissouriŻ 65212 } Phone (573) 882-8480 } FAX (573) 884-4100 } katzm-at-health.missouri.edu } Żhttp://www.muhealth.org/~ophthalmology/Katz.htm -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 25 -- From oshel1pe-at-cmich.edu Mon Feb 20 13:57:20 2006 5, 25 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 5, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KJvK7J029432 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 13:57:20 -0600 5, 25 -- Received: from egateb.central.cmich.local ([141.209.15.85]) 5, 25 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id k1KKaO4l026727; 5, 25 -- Mon, 20 Feb 2006 15:36:24 -0500 5, 25 -- Received: from [141.209.160.132] ([141.209.160.132]) by egateb.central.cmich.local with Microsoft SMTPSVC(5.0.2195.6713); 5, 25 -- Mon, 20 Feb 2006 14:57:16 -0500 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {f06230903c01fcdfc338c-at-[141.209.160.132]} 5, 25 -- In-Reply-To: {200602201925.k1KJPv8q027648-at-ns.microscopy.com} 5, 25 -- References: {200602201925.k1KJPv8q027648-at-ns.microscopy.com} 5, 25 -- Date: Mon, 20 Feb 2006 14:57:16 -0500 5, 25 -- To: TindallR-at-missouri.edu 5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 25 -- Subject: Re: [Microscopy] TEM: Cell culture preparation 5, 25 -- Cc: Microscopy-at-microscopy.com 5, 25 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 5, 25 -- X-OriginalArrivalTime: 20 Feb 2006 19:57:17.0011 (UTC) FILETIME=[D9C2BA30:01C63657] 5, 25 -- X-CanItPRO-Stream: default 5, 25 -- X-Spam-Score: -4 () L_EXCH_MF 5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1KJvK7J029432 ==============================End of - Headers==============================
Many (and I do mean many) years ago I fixed cultures of retinal pigment epithelial cells for TEM. I used a routine cacodylate-buffered glut. fix, no formaldehyde although that should not matter. I did put 1% tannic acid in the fix, both glut and osmium and it helped show the ECM but I don' think it should be essential. I grew the cells in ordinary culture dishes, no thermanox c'slips and embedded in Epon substitute. My guess is that your membranes look fuzzy due to less than optimal fixation of lipids (old glut and/or osmium?) or extraction of lipids (too long in dehydration?). Why are you using acetone and not ethanol? It is my understanding that acetone removes lipids faster than EtOH but I have also heard that the converse it true. Certainly too long in dehydration can degrade ultrastructure. Also, add 2mM Ca ions to the fix to help stabilize the lipids.
Geoff
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 7, 32 -- From mcauliff-at-umdnj.edu Mon Feb 20 14:25:41 2006 7, 32 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) 7, 32 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KKPfHB006954 7, 32 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 14:25:41 -0600 7, 32 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) 7, 32 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 058694BDF9 7, 32 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:25:41 -0500 (EST) 7, 32 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 7, 32 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 60A724BE2D 7, 32 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:25:40 -0500 (EST) 7, 32 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 7, 32 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 7, 32 -- id {0IV0009015TECC-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 7, 32 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 15:25:40 -0500 (EST) 7, 32 -- Received: from [127.0.0.1] ([10.138.2.240]) 7, 32 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 7, 32 -- 2004)) with ESMTP id {0IV0001KL62R6J-at-Polaris.umdnj.edu} ; Mon, 7, 32 -- 20 Feb 2006 15:25:39 -0500 (EST) 7, 32 -- Date: Mon, 20 Feb 2006 15:24:54 -0500 7, 32 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 7, 32 -- Subject: Re: [Microscopy] TEM: Cell culture preparation 7, 32 -- In-reply-to: {200602201921.k1KJLIov021601-at-ns.microscopy.com} 7, 32 -- To: TindallR-at-missouri.edu, 7, 32 -- MicroscopyListserver {microscopy-at-msa.microscopy.com} 7, 32 -- Message-id: {43FA2596.8050405-at-umdnj.edu} 7, 32 -- MIME-version: 1.0 7, 32 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 7, 32 -- Content-transfer-encoding: 7BIT 7, 32 -- X-Accept-Language: en-us, en 7, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 7, 32 -- Gecko/20040804 Netscape/7.2 (ax) 7, 32 -- References: {200602201921.k1KJLIov021601-at-ns.microscopy.com} ==============================End of - Headers==============================
I see that several people are using spam filters on this list. I have just received e-mail from 2 people supposedly on this list asking me to click on a link to verify that I am really sending them a non-spam e-mail. No way am I going to click on a link from someone I don't know! What ARE these people thinking?
Geoff
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 6, 29 -- From mcauliff-at-umdnj.edu Mon Feb 20 15:10:53 2006 6, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KLArhW017219 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:10:53 -0600 6, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 1F1151C327F 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:10:53 -0500 (EST) 6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 6, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id BEE40A7B41 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:10:51 -0500 (EST) 6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 6, 29 -- id {0IV000H017WH9Y-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 6, 29 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:10:51 -0500 (EST) 6, 29 -- Received: from [127.0.0.1] ([10.138.2.240]) 6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 6, 29 -- 2004)) with ESMTP id {0IV0001G880C6J-at-Polaris.umdnj.edu} for 6, 29 -- microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:07:24 -0500 (EST) 6, 29 -- Date: Mon, 20 Feb 2006 16:06:39 -0500 6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 6, 29 -- Subject: spam filters or ?? 6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com} 6, 29 -- Message-id: {43FA2F5F.7050807-at-umdnj.edu} 6, 29 -- MIME-version: 1.0 6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 6, 29 -- Content-transfer-encoding: 7BIT 6, 29 -- X-Accept-Language: en-us, en 6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 29 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
We recently saw a TIRF system demonstrated, and like other laser based TIRF systems, it seems to use a small peripheral arc aperture to allow the laser light through to part of the outside periphery of the 1.45NA objective. Why would you not use a complete ring annulus instead of an arc to illuminate the entire outside periphery of the objective? If you simply put the correct sized annulus into the field stop in the fluorescence path, would you get TIRF? Also, what is the theoretical advantage of using a laser as opposed to an arc illuminator. Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 3, 19 -- From david.knecht-at-uconn.edu Mon Feb 20 15:32:18 2006 3, 19 -- Received: from mail2.uits.uconn.edu (mail2.uits.uconn.edu [137.99.25.204]) 3, 19 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KLWITb026790 3, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:32:18 -0600 3, 19 -- Received: from [137.99.47.163] (d47h163.public.uconn.edu [137.99.47.163]) 3, 19 -- by mail2.uits.uconn.edu (8.11.6/8.11.6) with ESMTP id k1KLW0m26333 3, 19 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:32:00 -0500 3, 19 -- Mime-Version: 1.0 (Apple Message framework v746.2) 3, 19 -- Content-Transfer-Encoding: 7bit 3, 19 -- Message-Id: {8E45F94A-D08D-4926-9B2F-ECE8BF9D3920-at-uconn.edu} 3, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 3, 19 -- To: Microscopy-at-msa.microscopy.com 3, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 3, 19 -- Subject: TIRF setup 3, 19 -- Date: Mon, 20 Feb 2006 16:32:02 -0500 3, 19 -- X-Mailer: Apple Mail (2.746.2) 3, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 3, 19 -- X-UConn-MailScanner: Found to be clean 3, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
For the floor, there is a spray that can be put on it to temporarily stop electrostatic buildup. I'm sorry, but I can't remember the name of it, but I have used it in the past.
For electronic use, I found that taking my shoes off solves static discharge problems. There is enough moisture on my feet to stop any discharges.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: Monday, February 20, 2006 7:28 AM To: Walck-at-SouthBayTech.com
Tony
one thing that occurs to me is that you will probably have vinyl flooring in your darkroom. This may be a major source of static and it may be possible to get some advice on low static flooring. I know we had some installed in one of our microscope cubicles and it didn't seem to be tremendously expensive at the time.
If you think that the floor is part of the problem then it might be worth checking whether the soles of your shoes are man made (create static) or leather.
If you want to go for a tried and tested electronics industry route look for ionisers (rather than de-ionisers) I did a simple Google search on ioniser and static and came up with a few using keywords "ionisers static" such as http://www.buystatic.com/static_eliminators.htm?ioniser.htm
I was hoping that you could get away with a domestic ioniser - the sort they sell to simulate sea or mountain air and supposedly gets rid of headaches. But apparently they aren't much good at eliminating static whereas the industrial ones are.
Good luck
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK
e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: tonygr-at-MIT.EDU
Some of the solutions suggested seem to be based on the idea that the operator is picking up the static charge. I think the problem is much more likely to be caused by static on the film being discharged while unloading the exposed negatives. I now unload film wearing rubber gloves and rarely get a static discharge pattern on my film.
Ralph Common Dept. of Physiology Michigan State University
==============================Original Headers============================== 3, 25 -- From rcommon-at-msu.edu Mon Feb 20 16:01:59 2006 3, 25 -- Received: from sys15.mail.msu.edu (sys15.mail.msu.edu [35.9.75.115]) 3, 25 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KM1xUa013444 3, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Feb 2006 16:01:59 -0600 3, 25 -- Received: from [35.9.122.125] (helo=emlab) 3, 25 -- by sys15.mail.msu.edu with esmtpsa (Exim 4.52 #1) 3, 25 -- (TLSv1:RC4-MD5:128) 3, 25 -- id 1FBJ6U-0001zU-V8 3, 25 -- for Microscopy-at-microscopy.com; Mon, 20 Feb 2006 17:01:59 -0500 3, 25 -- From: "Ralph Common" {rcommon-at-msu.edu} 3, 25 -- To: {Microscopy-at-microscopy.com} 3, 25 -- Subject: TEM film electrostatic discharging 3, 25 -- Date: Mon, 20 Feb 2006 17:02:37 -0500 3, 25 -- Message-ID: {001401c63669$5dc89410$7d7a0923-at-msu.edu} 3, 25 -- MIME-Version: 1.0 3, 25 -- Content-Type: text/plain; 3, 25 -- charset="iso-8859-1" 3, 25 -- Content-Transfer-Encoding: 7bit 3, 25 -- X-Priority: 3 (Normal) 3, 25 -- X-MSMail-Priority: Normal 3, 25 -- X-Mailer: Microsoft Outlook CWS, Build 9.0.2416 (9.0.2911.0) 3, 25 -- In-Reply-To: {200602201528.k1KFSxtR005488-at-ns.microscopy.com} 3, 25 -- Importance: Normal 3, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1506 3, 25 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
Interesting question.... As I understand it the colors seen in thin sections are caused by destructive interference. The equation: Wavelength = 2nt/m describes the color seen. n = refractive index of film t is thickness (nm) M is any integer.
If so it seems that as refractive index decreases, wavelength will also decrease for the same thickness film.
I hope this help and thank you for giving me a chance to thumb through my old reference books, it brought back memories....
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==============================Original Headers============================== 9, 18 -- From frank.karl-at-degussa.com Tue Feb 21 07:09:25 2006 9, 18 -- Received: from mailout1.degussa.com (mailout1.degussa.com [193.100.56.173]) 9, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LD9OXV002077 9, 18 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 07:09:25 -0600 9, 18 -- Received: from mobuscomm01.mail.degussa.com (uss1026.applications.degussanet.com [172.20.6.74]) 9, 18 -- by mailout1.degussa.com (8.13.3/8.13.3/Debian-6) with SMTP id k1LD9B0P024169; 9, 18 -- Tue, 21 Feb 2006 14:09:17 +0100 9, 18 -- In-Reply-To: {200602172210.k1HMA6nj031675-at-ns.microscopy.com} 9, 18 -- Subject: Re: [Microscopy] viaWWW: microtome section thickness 9, 18 -- To: leis-at-biochem.mpg.de, microscopy-at-msa.microscopy.com 9, 18 -- X-Mailer: Lotus Notes Release 6.5.2 June 01, 2004 9, 18 -- Message-ID: {OFC1F9625B.D0797714-ON8525711C.004779CF-8525711C.004839F5-at-degussa.com} 9, 18 -- From: frank.karl-at-degussa.com 9, 18 -- Date: Tue, 21 Feb 2006 08:08:54 -0500 9, 18 -- X-MIMETrack: Serialize by Router on MOBUSComm01/DHexternal/US(Release 6.5.1|January 21, 2004) at 9, 18 -- 02/21/2006 07:09:18 AM 9, 18 -- MIME-Version: 1.0 9, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
I ignore these suspicious requests as well. I guess those folk don't get much mail now.
Dave
-----Original Message----- X-from: mcauliff-at-umdnj.edu [mailto:mcauliff-at-umdnj.edu] Sent: 20 February 2006 21:15 To: David Patton
Dear List;
I see that several people are using spam filters on this list. I have just received e-mail from 2 people supposedly on this list asking me to click on a link to verify that I am really sending them a non-spam
e-mail. No way am I going to click on a link from someone I don't know! What ARE these people thinking?
Geoff
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
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==============================Original Headers============================== 20, 33 -- From David.Patton-at-uwe.ac.uk Tue Feb 21 08:55:34 2006 20, 33 -- Received: from mailapp01.uwe.ac.uk (mailapp01.uwe.ac.uk [164.11.132.61]) 20, 33 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1LEtVGe013689 20, 33 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 08:55:33 -0600 20, 33 -- Received: from (164.11.132.60) by mailapp01.uwe.ac.uk via smtp 20, 33 -- id 4a43_94e119d4_a2ea_11da_89e4_0002b3c946e4; 20, 33 -- Tue, 21 Feb 2006 14:58:56 +0000 20, 33 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 20, 33 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 20, 33 -- by mta01.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 20, 33 -- 2005)) with ESMTP id {0IV1006AELGIO1-at-mta01.uwe.ac.uk} for 20, 33 -- microscopy-at-msa.microscopy.com; Tue, 21 Feb 2006 14:55:30 +0000 (GMT) 20, 33 -- Date: Tue, 21 Feb 2006 14:55:29 +0000 20, 33 -- From: David Patton {David.Patton-at-uwe.ac.uk} 20, 33 -- Subject: RE: [Microscopy] spam filters or ?? 20, 33 -- To: microscopy-at-msa.microscopy.com 20, 33 -- Message-id: {F247F674896BE243AD8263C5280E2BDB022BB804-at-egen-uwe01} 20, 33 -- MIME-version: 1.0 20, 33 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5.6944.0 20, 33 -- Content-type: text/plain; charset=us-ascii 20, 33 -- Content-class: urn:content-classes:message 20, 33 -- Thread-topic: [Microscopy] spam filters or ?? 20, 33 -- Thread-index: AcY2YzRIJ0lBMgRET9a4rhY9wkWYYgAk2+uQ 20, 33 -- X-MS-Has-Attach: 20, 33 -- X-MS-TNEF-Correlator: 20, 33 -- X-NAI-Spam-Level: * 20, 33 -- X-NAI-Spam-Score: 1.5 20, 33 -- X-NAI-Spam-Rules: 2 Rules triggered 20, 33 -- NAI_ZIP_CODE=4, BAYES_00=-2.5 20, 33 -- X-NAIMIME-Disclaimer: 1 20, 33 -- X-NAIMIME-Modified: 1 20, 33 -- Content-Transfer-Encoding: 8bit 20, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1LEtVGe013689 ==============================End of - Headers==============================
I have heard other people report situation like this and I, too, have experienced the same thing. Often time (not always) monolayer cells showed very little membrane contrast, even though the tissue processed same way had no problem. The problem with monolayer cells seemed random regardless what types of cells were being processed. More interestingly, I have not seen this problem with cell suspensions. In the past, I tried to use freshly made OsO4 once when I had low membrane contrast problem with monolayer cells, and that helped. But I still do not understand why the problem only occurs in monolayer cells. I do not think it is a reagent penetration issue, nor a problem of inadequate processing protocol. Is it possible that some kind of coating material used in culture makes it harder for OsO4 to react with lipid molecules? Thank you.
Hong Emory EM
==============================Original Headers============================== 5, 22 -- From hyi-at-emory.edu Tue Feb 21 10:06:29 2006 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222]) 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LG6S2K024016 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 10:06:28 -0600 5, 22 -- Received: from [170.140.232.202] (localhost [127.0.0.1]) 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k1LG6OIf028249 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 11:06:28 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) 5, 22 -- Message-Id: {5a5232b7e407dc1cfb2b84946528c088-at-emory.edu} 5, 22 -- Content-Type: text/plain; 5, 22 -- charset=ISO-8859-1; 5, 22 -- format=flowed 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- From: Hong Yi {hyi-at-emory.edu} 5, 22 -- Subject: Re: [Microscopy] TEM: Cell culture preparation 5, 22 -- Date: Tue, 21 Feb 2006 11:06:21 -0500 5, 22 -- X-Mailer: Apple Mail (2.622) 5, 22 -- X-imss-version: 2.037 5, 22 -- X-imss-result: Passed 5, 22 -- X-imss-approveListMatch: *-at-emory.edu 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1LG6S2K024016 ==============================End of - Headers==============================
I process monolayers of cells frequently for TEM. The problem of low membrane contrast is due to the lipids being extracted away during dehydration and embedding. Even membranes "stabilized" with OsO4 can be extracted with the long processing times used during "standard fixations". I typically dehydrate in EtOH for 1-2 minutes per EtOH grade, with my samples dehydrated from 100% water to 100% EtOH in 15-20 minutes. I also keep the time in liquid resin to a minimum...my whole embedding protocol takes less than 3 hours. After I shortened my times considerably, my membranes started looking very nice and crisp! Good Luck -Eugene
Eugene W. A. Krueger Sr. Research Technologist in Cell Biology II Center for Basic Research in Digestive Diseases Mayo Clinic and Foundation (507)284-0580 (lab) (507)284-0762 (fax) krueger.eugene-at-mayo.edu
} Hi, Randy: } } I have heard other people report situation like this and I, } too, have experienced the same thing. Often time (not always) monolayer } cells showed very little membrane contrast, even though the tissue } processed same way had no problem. The problem with monolayer cells } seemed random regardless what types of cells were being processed. More } interestingly, I have not seen this problem with cell suspensions. In } the past, I tried to use freshly made OsO4 once when I had low membrane } contrast problem with monolayer cells, and that helped. But I still do } not understand why the problem only occurs in monolayer cells. I do not } think it is a reagent penetration issue, nor a problem of inadequate } processing protocol. Is it possible that some kind of coating material } used in culture makes it harder for OsO4 to react with lipid } molecules? } Thank you. } } Hong } Emory EM } } } } } ==============================Original Headers============================== } 5, 22 -- From hyi-at-emory.edu Tue Feb 21 10:06:29 2006 } 5, 22 -- Received: from virginia.cc.emory.edu (virginia.cc.emory.edu [170.140.8.222]) } 5, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LG6S2K024016 } 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 10:06:28 -0600 } 5, 22 -- Received: from [170.140.232.202] (localhost [127.0.0.1]) } 5, 22 -- by virginia.cc.emory.edu (8.13.4/8.13.4) with ESMTP id k1LG6OIf028249 } 5, 22 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 11:06:28 -0500 (EST) } 5, 22 -- Mime-Version: 1.0 (Apple Message framework v622) } 5, 22 -- Message-Id: {5a5232b7e407dc1cfb2b84946528c088-at-emory.edu} } 5, 22 -- Content-Type: text/plain; } 5, 22 -- charset=ISO-8859-1; } 5, 22 -- format=flowed } 5, 22 -- To: microscopy-at-microscopy.com } 5, 22 -- From: Hong Yi {hyi-at-emory.edu} } 5, 22 -- Subject: Re: [Microscopy] TEM: Cell culture preparation } 5, 22 -- Date: Tue, 21 Feb 2006 11:06:21 -0500 } 5, 22 -- X-Mailer: Apple Mail (2.622) } 5, 22 -- X-imss-version: 2.037 } 5, 22 -- X-imss-result: Passed } 5, 22 -- X-imss-approveListMatch: *-at-emory.edu } 5, 22 -- Content-Transfer-Encoding: 8bit } 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id k1LG6S2K024016 } ==============================End of - Headers============================== } }
==============================Original Headers============================== 6, 20 -- From krueger.eugene-at-mayo.edu Tue Feb 21 10:56:47 2006 6, 20 -- Received: from mail1.mayo.edu (mail1.mayo.edu [129.176.212.12]) 6, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LGuloa001993 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 10:56:47 -0600 6, 20 -- Received: from mhro1a.mayo.edu ([129.176.212.53]) 6, 20 -- by mail1.mayo.edu with ESMTP; 21 Feb 2006 10:56:46 -0600 6, 20 -- X-BrightmailFiltered: true 6, 20 -- X-Brightmail-Tracker: AAAAAA== 6, 20 -- Received: from excsrv01.mayo.edu (excsrv01.mayo.edu [129.176.235.101]) by mhro1a.mayo.edu with ESMTP for Microscopy-at-microscopy.com; Tue, 21 Feb 2006 10:56:46 -0600 6, 20 -- Received: by excsrv01.mayo.edu with Internet Mail Service (5.5.2657.72) 6, 20 -- id {FMHXTMZG} ; Tue, 21 Feb 2006 10:53:21 -0600 6, 20 -- Message-Id: {FC1B895EA707A940A6BD5F74106FAA9A269497-at-excsrv80.mayo.edu} 6, 20 -- From: "Krueger, Eugene W." {krueger.eugene-at-mayo.edu} 6, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 6, 20 -- Subject: Re: TEM: Cell culture preparation 6, 20 -- Date: Tue, 21 Feb 2006 10:56:42 -0600 6, 20 -- MIME-Version: 1.0 6, 20 -- X-Mailer: Internet Mail Service (5.5.2657.72) 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
On Feb 18, 2006, at 5:54 AM, tonygr-at-MIT.EDU wrote:
} This is a perennial problem, I know, but does anyone have any } effective "pet" solutions to the problem of charging and subsequent } electrostatic arcing of TEM film in the extremely dry air we get in } New England typically in the winter, and some other people get at } other seasons of the year? } } The discharges can occur at almost any stage of the handling, from } getting the film out of the vendor's packing, loading and unloading in } the microscope carriers and loading in the developing racks (we use } Lucite ones). We have considered a humidifier in the darkroom, but } that would involve blocking off the ventilation (we have make-up air } positively blown into to room and exhaust actively sucked out) to } prevent our moisture from being simply blown away. } } Any hints would be welcome, but switching to digital imaging is one } that is not going to happen. } Dear Tony, When removing the film from the envelopes, keep it in a pack, so none of the films rubs along any other, then lift the cardboard without rubbing. If you can maintain contact with a grounded metal surface--the water pipes are good--this will help a lot. Try not to let your lab coat (or other clothing for that matter) to move along your body or other items of clothing. Always separate films by bending the top one away from the others, then lifting. Trade your lucite racks for metal ones. These procedures reduced, but did not completely eliminate, the problem when I was in Albany. When using LoDose film in total darkness, I could at least see the discharges when they occurred. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Feb 21 13:32:32 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LJWVHu013187 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 13:32:32 -0600 4, 22 -- Received: from localhost (earth-dog [192.168.1.3]) 4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 5719E35BB9 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 11:32:31 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 2D62435A54 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 11:32:30 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200602181354.k1IDsSQp003552-at-ns.microscopy.com} 4, 22 -- References: {200602181354.k1IDsSQp003552-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {8eab6d7bd81e0fcbc6fc662ef0c1c9fa-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] TEM film electrostatic discharging 4, 22 -- Date: Tue, 21 Feb 2006 11:40:34 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.3.3 ==============================End of - Headers==============================
We have a vintage Sorvall MT-1 ultramicrotome available for the cost of shipping. Manual included. Has no stereomicroscope with it. Great for semithin plastic sections. All manual operation. It is headed for the junk pile if no one adopts it.
-- Gregory W. Erdos, Ph.D, Assistant Director, Biotechnology Program Scientific Director, EM Core Lab P.O. Box 118525 University of Florida Gainesville, FL 32611 gwe-at-ufl.edu Phone: 352-392-1295 Fax: 352-846-0251
==============================Original Headers============================== 3, 22 -- From gwe-at-ufl.edu Tue Feb 21 14:18:40 2006 3, 22 -- Received: from smtp.ufl.edu (smtp01.osg.ufl.edu [128.227.74.149]) 3, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LKIeL1023349 3, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 14:18:40 -0600 3, 22 -- Received: from [10.227.60.63] (empc14419.dhcp.clas.ufl.edu [10.227.60.63]) 3, 22 -- (authenticated bits=0) 3, 22 -- by smtp.ufl.edu (8.13.4/8.13.4/2.5.1) with ESMTP id k1LKIacU4337810 3, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 15:18:37 -0500 3, 22 -- Message-ID: {43FB759D.6050002-at-ufl.edu} 3, 22 -- Date: Tue, 21 Feb 2006 15:18:37 -0500 3, 22 -- From: Greg Erdos {gwe-at-ufl.edu} 3, 22 -- User-Agent: Mozilla Thunderbird 1.0.7 (Windows/20050923) 3, 22 -- X-Accept-Language: en-us, en 3, 22 -- MIME-Version: 1.0 3, 22 -- To: "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-MSA.Microscopy.Com} 3, 22 -- Subject: MT-1 for free 3, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 22 -- Content-Transfer-Encoding: 7bit 3, 22 -- X-Spam-Status: hits=-0.904, required=5, tests=BAYES_30 3, 22 -- X-UFL-Spam-Status: hits=-0.904, required=5, tests=BAYES_30 3, 22 -- X-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) 3, 22 -- X-UFL-Scanned-By: CNS Open Systems Group (http://open-systems.ufl.edu/services/smtp-relay/) ==============================End of - Headers==============================
I was asked to post my embedding schedule, so here it is:
When embedding monolayers (~60 - 90% confluent) in epoxy resin (I prefer Quetol 651) I follow this schedule:
graded series of EtOH, 25%, 50%, 70%, 95%, 100%, 100%, total dehydration time 15-20' 100%EtOH:Quetol 651 (1:1), ~25' 100% Quetol 651, ~50' 100% Quetol 651, ~100' fresh 100% Quetol 651, then into 60C oven
I do my processing in a 6 or 12 well plate that sits on a shelf in the hood (NOT on a rotator), and I change to a new 6 or 12 well plate between changes of 100% EtOH. I also use minimal volumes of resin to decrease extraction of lipid components. If you use a more viscous resin, times may need to be adjusted longer. Good Luck!
-Eugene Eugene W. A. Krueger Sr. Research Technologist in Cell Biology II Center for Basic Research in Digestive Diseases Mayo Clinic and Foundation (507)284-0580 (lab) (507)284-0762 (fax) krueger.eugene-at-mayo.edu
==============================Original Headers============================== 5, 20 -- From krueger.eugene-at-mayo.edu Tue Feb 21 14:47:11 2006 5, 20 -- Received: from mail2.mayo.edu (mail2.mayo.edu [129.176.212.15]) 5, 20 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1LKlBv7003576 5, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 21 Feb 2006 14:47:11 -0600 5, 20 -- Received: from mhro1a.mayo.edu ([129.176.212.53]) 5, 20 -- by mail2.mayo.edu with ESMTP; 21 Feb 2006 14:47:10 -0600 5, 20 -- X-BrightmailFiltered: true 5, 20 -- X-Brightmail-Tracker: AAAAAA== 5, 20 -- Received: from excsrv01.mayo.edu (excsrv01.mayo.edu [129.176.235.101]) by mhro1a.mayo.edu with ESMTP for Microscopy-at-microscopy.com; Tue, 21 Feb 2006 14:47:10 -0600 5, 20 -- Received: by excsrv01.mayo.edu with Internet Mail Service (5.5.2657.72) 5, 20 -- id {FMHXW34R} ; Tue, 21 Feb 2006 14:43:45 -0600 5, 20 -- Message-Id: {FC1B895EA707A940A6BD5F74106FAA9A269498-at-excsrv80.mayo.edu} 5, 20 -- From: "Krueger, Eugene W." {krueger.eugene-at-mayo.edu} 5, 20 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 5, 20 -- Subject: embedding schedule for monolayers 5, 20 -- Date: Tue, 21 Feb 2006 14:47:07 -0600 5, 20 -- MIME-Version: 1.0 5, 20 -- X-Mailer: Internet Mail Service (5.5.2657.72) 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" ==============================End of - Headers==============================
On Feb 18, 2006, at 2:32 PM, soleimanij-at-tbzmed.ac.ir wrote:
} we are using A LEO 906 TEM, scale bar is not registered in the } microfilms. We are having problem to calculating the real sizes. any } help in this matter is appreciated. } Dear Jafar, I would calibrate the magnification(s) of interest either with a cross-grating replica or, preferably, a Mag*I*Cal, both of which are available from many supply houses. If you need sizes on prints, there is a calibration slide that can be photographed in an enlarger, with which you can determine the ratio of print sizes to negative sizes; I don't know where to get this, but it must be widely available. Yours, Bill Tivol, PhD EM Scientist and Manager Cryo-Electron Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 22 -- From tivol-at-caltech.edu Tue Feb 21 18:39:28 2006 4, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 22 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1M0dSPK023528 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 18:39:28 -0600 4, 22 -- Received: from localhost (water-dog [192.168.1.26]) 4, 22 -- by fire-ox-postvirus (Postfix) with ESMTP id 63DDE359F6 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 16:39:28 -0800 (PST) 4, 22 -- Received: from [192.168.157.234] (pix-1.caltech.edu [131.215.2.21]) 4, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id AF21035C10 4, 22 -- for {microscopy-at-msa.microscopy.com} ; Tue, 21 Feb 2006 16:39:16 -0800 (PST) 4, 22 -- Mime-Version: 1.0 (Apple Message framework v623) 4, 22 -- In-Reply-To: {200602182232.k1IMW6Zs027921-at-ns.microscopy.com} 4, 22 -- References: {200602182232.k1IMW6Zs027921-at-ns.microscopy.com} 4, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 4, 22 -- Message-Id: {7bf40a7674c34bd31ebb3e601deaaae1-at-caltech.edu} 4, 22 -- Content-Transfer-Encoding: 7bit 4, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 22 -- Subject: Re: [Microscopy] viaWWW: scale bar 4, 22 -- Date: Tue, 21 Feb 2006 16:47:18 -0800 4, 22 -- To: microscopy-at-msa.microscopy.com 4, 22 -- X-Mailer: Apple Mail (2.623) 4, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.3.3 ==============================End of - Headers==============================
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Dear List;
I see that several people are using spam filters on this list. I have just received e-mail from 2 people supposedly on this list asking me to click on a link to verify that I am really sending them a non-spam e-mail. No way am I going to click on a link from someone I don't know! What ARE these people thinking?
Geoff
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 6, 29 -- From mcauliff-at-umdnj.edu Mon Feb 20 15:10:53 2006 6, 29 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 6, 29 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1KLArhW017219 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 15:10:53 -0600 6, 29 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 6, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 1F1151C327F 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:10:53 -0500 (EST) 6, 29 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 6, 29 -- by zix01.umdnj.edu (Proprietary) with ESMTP id BEE40A7B41 6, 29 -- for {microscopy-at-msa.microscopy.com} ; Mon, 20 Feb 2006 16:10:51 -0500 (EST) 6, 29 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 6, 29 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 6, 29 -- id {0IV000H017WH9Y-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 6, 29 -- for microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:10:51 -0500 (EST) 6, 29 -- Received: from [127.0.0.1] ([10.138.2.240]) 6, 29 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 6, 29 -- 2004)) with ESMTP id {0IV0001G880C6J-at-Polaris.umdnj.edu} for 6, 29 -- microscopy-at-msa.microscopy.com; Mon, 20 Feb 2006 16:07:24 -0500 (EST) 6, 29 -- Date: Mon, 20 Feb 2006 16:06:39 -0500 6, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 6, 29 -- Subject: spam filters or ?? 6, 29 -- To: MicroscopyListserver {microscopy-at-msa.microscopy.com} 6, 29 -- Message-id: {43FA2F5F.7050807-at-umdnj.edu} 6, 29 -- MIME-version: 1.0 6, 29 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 6, 29 -- Content-transfer-encoding: 7BIT 6, 29 -- X-Accept-Language: en-us, en 6, 29 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 6, 29 -- Gecko/20040804 Netscape/7.2 (ax) ==============================End of - Headers==============================
==============================Original Headers============================== 23, 27 -- From milesd-at-us.ibm.com Tue Feb 21 20:05:07 2006 23, 27 -- Received: from e3.ny.us.ibm.com (e3.ny.us.ibm.com [32.97.182.143]) 23, 27 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1M257Q0001451 23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 20:05:07 -0600 23, 27 -- Received: from d01relay04.pok.ibm.com (d01relay04.pok.ibm.com [9.56.227.236]) 23, 27 -- by e3.ny.us.ibm.com (8.12.11/8.12.11) with ESMTP id k1M255Vc016162 23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:05 -0500 23, 27 -- Received: from d01av01.pok.ibm.com (d01av01.pok.ibm.com [9.56.224.215]) 23, 27 -- by d01relay04.pok.ibm.com (8.12.10/NCO/VERS6.8) with ESMTP id k1M2562i220526 23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:06 -0500 23, 27 -- Received: from d01av01.pok.ibm.com (loopback [127.0.0.1]) 23, 27 -- by d01av01.pok.ibm.com (8.12.11/8.13.3) with ESMTP id k1M255LU008116 23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:05 -0500 23, 27 -- Received: from d01ml076.pok.ibm.com (d01ml076.pok.ibm.com [9.56.229.50]) 23, 27 -- by d01av01.pok.ibm.com (8.12.11/8.12.11) with ESMTP id k1M2540P008094 23, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 21:05:04 -0500 23, 27 -- In-Reply-To: {200602202111.k1KLBUjW018639-at-ns.microscopy.com} 23, 27 -- Subject: Re: [Microscopy] spam filters or ?? 23, 27 -- To: Microscopy-at-MSA.Microscopy.Com 23, 27 -- X-Mailer: Lotus Notes Release 7.0 HF85 November 04, 2005 23, 27 -- Message-ID: {OF22CCB88F.52878652-ON8525711D.000B000E-8525711D.000B7274-at-us.ibm.com} 23, 27 -- From: Darrell Miles {milesd-at-us.ibm.com} 23, 27 -- Date: Tue, 21 Feb 2006 21:05:03 -0500 23, 27 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 6.5.4FP2 HF4|December 20, 2005) at 23, 27 -- 02/21/2006 21:05:04 23, 27 -- MIME-Version: 1.0 23, 27 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
I'm looking for a reliable scanner for TEM Micrographs. For the past 4 years I used an old HP scanner that was very good with 35mm and TEM micrographs, unfortunate this scanner is no longer working, now I need a scanner that can capture a reliable image for routine everyday micrographs; in order to shorten darkroom time. Any subjection?
Thanks in advance
Omayra Velez Life Cell Branchburg, NJ
==============================Original Headers============================== 5, 18 -- From mayas003-at-yahoo.com Tue Feb 21 22:58:21 2006 5, 18 -- Received: from web84109.mail.dcn.yahoo.com (web84109.mail.dcn.yahoo.com [209.73.179.120]) 5, 18 -- by ns.microscopy.com (8.12.11/8.12.8) with SMTP id k1M4wK2N012775 5, 18 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 21 Feb 2006 22:58:20 -0600 5, 18 -- Received: (qmail 63646 invoked by uid 60001); 22 Feb 2006 04:58:20 -0000 5, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 18 -- s=s1024; d=yahoo.com; 5, 18 -- h=Message-ID:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 18 -- b=l3I3qltvjW7C7MFLKskZiUVjJbPQ1cydee1f6xuI3x0YEOtyb1XLN+xZX7pO4ne8HOWyjClZfM4jfomwRvPZ1aIimFvPffyyv5mgzkS65fZVHYy/qLK30Kq3Ft6gNcfeVuPoHIlCg5oBNKSj3juJH9BgGJ/Hx0brg5OPd0fZ8gY= ; 5, 18 -- Message-ID: {20060222045820.63644.qmail-at-web84109.mail.dcn.yahoo.com} 5, 18 -- Received: from [68.239.240.252] by web84109.mail.dcn.yahoo.com via HTTP; Tue, 21 Feb 2006 20:58:20 PST 5, 18 -- Date: Tue, 21 Feb 2006 20:58:20 -0800 (PST) 5, 18 -- From: Omayra Velez {mayas003-at-yahoo.com} 5, 18 -- Subject: Scanner for TEM Micrographs 5, 18 -- To: Microscopy-at-MSA.Microscopy.Com 5, 18 -- MIME-Version: 1.0 5, 18 -- Content-Type: text/plain; charset=iso-8859-1 5, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Like microscopes, it really is the more you pay the better the result (even my kids £60 DX5 microscope has 250x magnification and a CMOS 'camera').
However, from my experience even going to £2,000 for a scanner, the result of a negative or slide is sometimes not as good as the original if you really start magnifying it up (although I haven't tried the latest 4000 to 5000 dpi top of the range Nikon slide scanners). This is mainly due to film grain size optical effects that even Digital GEM doesn't seem to wholly eliminate (it is just a software image processing system even if embedded into the scanner hardware & optimised for the scanner). Grain size optical effects are such that a 400ASA negative colour scan at 2,700dpi can produce an appallingly unusable image that image processing can't really save - garbage in garbage out (whereas a reflective scan of the 6x4" print produces a very reasonable A4 image). Higher resolutions approaching or exceeding the grain size of the file (e.g. 4000 dpi and above) are supposed to reduce this problem considerably but Nikon type slide scanners are expensive and very limited in negative size options. In practice many problems in image quality are as much due to ease of magnifying a digital image compared to the negative - a few clicks and you have a 'print' the size of a wall.
However the scanned slide image from lower ASA 50-100 slides at 4000dpi is probably better on the VDU than most standard (not enlargements) prints made from the negative/slide and it can easily go up to A3 in output to a printer, which is probably all that really matters (and you still have the negative in the likely event the next generation scanners will get even better for the price). However enlargement prints from the negative may be better than a magnified scanned image. Naturally if you want to zoom in on a negative, it would have been better to do this on the TEM in the first place and take another picture instead (wise after the event).
In practice it might also be that our eyes prefer a fuzzy analogue gradation of colour rather than the very defined little squares of a pixellated image at the same resolution. We are also good at discerning contrast. Also remember that digital camera's often do some image processing during capture (e.g. noise reduction, colour correction and sometimes even sharpen) so you have to work on the image after scanning to get the same result. Top of the range scanner software and hardware can do this 'automatically' using calibration targets and so forth (e.g. Digital GEM, SHO, ICE and Silverfast Ai). This scan processing can triple scan times to 10 minutes or more, but can save far more than that on manual Photoshop type processing times afterwards (and make a better job of it). Note that we can only discern 191 grey levels so 8-bit (256 grey levels) B&W scans be fine for most uses except perhaps image analysis - and this really reduced image file size.
So the scanned images can look very good at A3 and on a 22" monitor, and if your negative archive is going the way of my collection of 1950's 35mm home slides and literally disintegrating into brown mush, anything is an improvement. Surprisingly the twain software can also have an effect on scanned quality in some cases (cheaper slide scanners often benefit from using Silverfast SE over the cheaper bundled software).
To scan many large negatives at high resolution via a 5000dpi Creo type hi-resolution flatbed scanner would need an investment of £12k to £45 and the massive files would be hard to manage and archive without image size reduction and compression which renders the whole procedure rather pointless. Given the cost it would seem to be preferable to pay someone to scan the odd few negatives you need scanned at this resolution with their Creo. Hi-resolution drum scanners up to 1500dpi cost nearer £70k but if your collection is something like NASA's archive that price can be well worth paying (see their results at http://grin.hq.nasa.gov). A 2k x 2k or greater pixel image digital camera system for a TEM costs around £20k to £70k.
You can get pretty good results from the new breed of sub-£1,000 flatbed scanners though, particularly with large format negatives (i.e. TEM). Have a look at the reviews of these semi-pro flatbed scanners on the net (around £300 to 800 to buy). Below is an excellent link for the Canon 9950F that could be used for 4x5" or 9 x 6cm film. It also compares the results with the similar Epson 4990 Photo.
http://www.photo-i.co.uk
I use the Canon 9950F (£260) at home for 35mm negatives and slides. The Canon's main weakness is that it's twain interface can only scan to the frame sizes (i.e. not to my square Kodak 125 slides, where it chops on the top and bottom of the slides to fit it to 35mm - a real pain). With Silverfast SE [twain] also provided, you can scan any film size. However Canon won't share the FARE dust removal technology with Silverfast so FARE isn't supported - not a problem with B&W negatives as FARE and ICE only work with colour - although you can scan B&W negatives in 'colour' with FARE/ICE and convert to B&W in Photoshop - but 'results may vary'. I have upgraded to Silverfast Ai for $115 for my Canon 9950F which adds in auto multi-slide scans (but not FARE). Canon no longer manufacture dedicated slide scanners as they feel this flatbed is as good.
At work I use the Epson 4990 (£300). It has a better twain interface and has an A4 negative scan feature that can scan any smaller negatives in multiples if they don't fit the standard frames provided. Silverfast SE also provided supports ICE infra-red dust removal - but dust removal can reduce image quality a little if the negative isn't that dusty. I use a standard rubber bulb to blow dust off before scanning - air jets canisters are gone in a day and can squirt propellant over the film. It's image quality is identical to Canon 9950F for 35mm slides, although the Epson can only scan 8 slides at one go to the Canon's 12. Scan time is similar for both (very slow with FARE/ICE - about 10 minutes a 35mm slide). However both these flatbeds are really 2,400 dpi not 4800 dpi as claimed - if you scan 35mm at 4800 dpi the image quality is virtually identical the 2,400 dpi scan. However you can't regularly scan full TEM at 4,800 dpi and above as the image size would be near 1 Gb - we scan at 800 to 1,200 dpi mainly - although you can of course scan small areas at 2,400 dpi resolution for 'enlargements'. All uses say they are happy with the TEM scan quality.
If you keep the negative anyway, I would have thought being able to print to A3 size is adequate for most purposes. The cheap Canon 9950F flatbed has replaced my 2,700 dpi SCSI Scanwit 2740s dedicated slide scanner at home (largely as it's so much faster and easier to use). I have to say the image quality of these flatbeds is a little out of focus (or 'soft' as we call it) at full magnification, but the careful use of USM (unsharp mask) in Photoshop can improve this a lot. But they are fine up to A3 at least (from a 35mm slide). Flatbed scans always need a little more Twain and post scan tweeking than dedicated film scanners. Leave things like USM and colour balance to Photoshop but use the twain interface to set things like brightness in dark negatives and dust ICE/FARE removal.
These scanners can come with expensive Silverfast Ai and targets though (sometimes as a Pro version) or you can upgrade at silverfast.com. But a lot of this is for accurate colour (not TEM's strong point) - although Silverfast is a powerful & complicated Twain interface.
When going from 2,700 dpi of the old slide/negative scanners up to the 4,000 dpi of modern Nikon type slide/negative scanners many users report far better image quality, and put it down to reduced effects from grain aliasing e.g. http://hardware.mcse.ms/message144915.html and presumably the same will be true of TEM negatives and the latest 4,000 dpi semi-pro flatbed scanners (that can take 4"x5") as well. At these resolutions film grain is also very apparent though, as 35mm film grain has a lower dpi.
There's also dedicated & cheap large film scanners like the Epson F3200 going to 4x5" film but again the resolution of the F3200 is quoted at 'only' 3200dpi, plus it got a duff review : http://www.photo-i.co.uk/Reviews/interactive/Scanners/Epson_F3200/page-1.htm
In fact it is probably a little better for image quality (prior to USM) than the flatbeds for large B&W negatives, but you may be limited to the frame sizes so check if you have 6x9cm negatives rather than proper ¼ plate. It seems that it can scan any size smaller than the largest frame - but certainly there's no A4 negative or reflective scanning.
Have a look at: http://www.photoscientia.co.uk/Grain.htm for discussions of grain size.
Have a look at http://www.datamind.co.uk/merchant/resolution.htm for some chat on pixel and image file sizes.
Hope this is of some use.
Regards
Keith
PS. This a bit large to post on the listerver, but it gave me something to do while travelling for 4 hours on the train to & from work each day. This follows on from similar threads about scanner a few months ago.
----- Original Message ----- X-from: {mayas003-at-yahoo.com} To: {keith.morris-at-ucl.ac.uk} Sent: Wednesday, February 22, 2006 5:02 AM
I put my EM negatives on a light box, mask off all other areas with black paper (so 'extra' light does not mislead the light meter), copy with my digital camera, then invert the image to a positive and convert to grayscale in PhotoShop. Fast, easy and excellent results.
Geoff
mayas003-at-yahoo.com wrote:
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-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 7, 31 -- From mcauliff-at-umdnj.edu Wed Feb 22 09:02:53 2006 7, 31 -- Received: from zix02.umdnj.edu (zix02.UMDNJ.EDU [130.219.34.125]) 7, 31 -- by ns.microscopy.com (8.12.11/8.12.8) with ESMTP id k1MF2qZ5007257 7, 31 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Feb 2006 09:02:52 -0600 7, 31 -- Received: from zix02.umdnj.edu (ZixVPM [127.0.0.1]) 7, 31 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 586FC4BE3C 7, 31 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Feb 2006 09:02:52 -0600 (CST) 7, 31 -- Received: from polaris.umdnj.edu (polarisa.UMDNJ.EDU [130.219.34.131]) 7, 31 -- by zix02.umdnj.edu (Proprietary) with ESMTP id 2EB6F4BE34 7, 31 -- for {microscopy-at-msa.microscopy.com} ; Wed, 22 Feb 2006 09:02:51 -0600 (CST) 7, 31 -- Received: from conversion-daemon.Polaris.umdnj.edu by Polaris.umdnj.edu 7, 31 -- (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 2004)) 7, 31 -- id {0IV300E01GF9ER-at-Polaris.umdnj.edu} (original mail from mcauliff-at-umdnj.edu) 7, 31 -- for microscopy-at-msa.microscopy.com; Wed, 22 Feb 2006 10:02:50 -0500 (EST) 7, 31 -- Received: from [127.0.0.1] ([10.138.2.240]) 7, 31 -- by Polaris.umdnj.edu (iPlanet Messaging Server 5.2 HotFix 2.02 (built Oct 21 7, 31 -- 2004)) with ESMTP id {0IV300H8HGBMQ5-at-Polaris.umdnj.edu} ; Wed, 7, 31 -- 22 Feb 2006 09:59:46 -0500 (EST) 7, 31 -- Date: Wed, 22 Feb 2006 09:59:01 -0500 7, 31 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 7, 31 -- Subject: Re: [Microscopy] Scanner for TEM Micrographs 7, 31 -- In-reply-to: {200602220459.k1M4xdY3014788-at-ns.microscopy.com} 7, 31 -- To: mayas003-at-yahoo.com, MicroscopyListserver {microscopy-at-msa.microscopy.com} 7, 31 -- Message-id: {43FC7C35.5050601-at-umdnj.edu} 7, 31 -- MIME-version: 1.0 7, 31 -- Content-type: text/plain; format=flowed; charset=us-ascii 7, 31 -- Content-transfer-encoding: 7BIT 7, 31 -- X-Accept-Language: en-us, en 7, 31 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) 7, 31 -- Gecko/20040804 Netscape/7.2 (ax) 7, 31 -- References: {200602220459.k1M4xdY3014788-at-ns.microscopy.com} ==============================End of - Headers==============================
I am not sure why these would not be legitimate. I have gotten such requests that cited my message of some date and some subject, so they already have my e-mail address. I can easily envision an anti-spam service where a person must respond to such a challenge to get added to a white-list of senders. I have replied to a couple of those posts as instructed and have not been asked to re-register.
However, I also sent them a note that such a service is not very compatible with membership in a list server like this. Everyone who ever posts to the list will get challenged to register their e-mail address. I doubt that many will. And if I declined to register and continued to get these registration requests, I would probably add that person to my junk mailers list.
If someone halfway around the world doesn't want to accept the knowledge that gets shared via this list then that is their problem. They should not use such a challenge system on the e-mail address they use for this or other listservers.
from previous replies on the list it seems to come down to about 3-4 basic types of film scanner: 1. Very expensive dedicated drum scanners 2. Standard dedicated film scanners like the Nikon 3. A combined flatbed and glassless scanner (Agfa used to make the Duo- scan) 4. Standard flatbed scanner with glass.
I think that Keith has covered everything but the 3. combined scanner. I use a Microtek Scanmaker 8700 and find it useful because there is no glass between the optics and the film which should reduce Newton's Rings, dust and finger marks. The new Microtek is cheaper and more powerful than mine was. It handles true 3200 x 6400 dpi scans, 4.2 Dmax negatives, 48 bit colour and sells for under 700 UK pounds or about 600 US dollars. I think it still comes with Silversoft scanning software and Digital ICE (scratc